LOCUS       DANJWC010000001        42338 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.001, whole genome shotgun sequence.
ACCESSION   DANJWC010000001 DANJWC010000000
VERSION     DANJWC010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 42338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42338
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.001"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            151..654
                     /locus_tag="PWX02_000001"
     CDS             151..654
                     /locus_tag="PWX02_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HDL1728066.1"
                     /translation="MNQSYFNLLGNITWLWMNSSLHKEWSCELLARNVIPAIENEQYM
                     LLIDNGIPIAYCSWADLNLETEVKYIKDISSLTPEEWQSGDRRWIIDWVAPFGHSQLL
                     YKKMCQKYADTLVRSIRFQPNQKSVGKIAYFKGGKLDKKTAKKRFDTYQEELATALKN
                     EFNFIKK"
     gene            670..3531
                     /locus_tag="PWX02_000002"
     CDS             670..3531
                     /locus_tag="PWX02_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX family hemolysin"
                     /protein_id="HDL1728067.1"
                     /translation="MGNKLTNISTNLKSSWLTAKSGLNRTGQSLAKAGQSLKTGAKKI
                     ILYIPKDYQYDTDKGNGLQDLVKAAEELGIEVQKEESNDIAKAQTSLGTIHNVLGLTE
                     RGIVLSAPQLDKLLQKTKVGQAIGSTENITKGFSNAKTVLSGIQSILGSVLAGMDLDE
                     ALQNNSNELTLAKAGLELTNSLIENIANSVKTLDAFGDQINQLGSKLQNVKGLSSLGE
                     KLKGLSGFDKTSLGLDIVSGLLSGATAALVLADKNASTSRKVGAGFELANQVVGNITK
                     AVSSYILAQRVAAGLSSTGPVAALIASTVSLAISPLSFAGIADKFNHAKSLESYAERF
                     KKLGYDGDNLLAEYQRGTGTIDASVTAINTALAAIAGGVSAAAAGSVVASPIALLVSG
                     ITGVISTILQYSKQAMFEHVANKIHNKIVEWEKNNPGKNYFENGYDARYLANLQDNMK
                     FLLNLNKELQAERVIAITQQQWDNNIGDLAGISRLGEKVLSGKAYVDAFEEGQHLKAD
                     KLVQLDSAKGIIDVSNTGEAKTQHILFRTPLLTPGTEKRERVQTGKYEYITKLHINRV
                     DSWKITDGAASSTFDLTNVVQRIGIELDNAGNVTKTKETKIIAKLGEGDDNVFVGSGT
                     TEIDGGEGYDRVHYSRGNYGALTIDATKETEQGSYTVNRFVESGKALHEVTSTHTALV
                     GNREEKIEYRHSNNQHHAGYYTKDTLKAVEEIIGTSHNDIFKGSKFNDAFNGGDGVDT
                     IDGNDGNDRLFGGKGDDIIDGGNGDDFIDGGKGNDLLHGGKGDDIFVHRQGDGNDSIT
                     ESEGNDKLSFSDSNLKDLTFEKVNHHLVITNTKQEKVTIQNWFREAEFAKTIQNYVAT
                     RDDKIEEIIGQNGERITSKQVDELIEKGNGKIAQSELTKVVDNYQLLKYSRDASNSLD
                     KLISSASAFTSSNDSRNVLASPTSMLDPSLSSIQFARAA"
     gene            3606..5732
                     /locus_tag="PWX02_000003"
     CDS             3606..5732
                     /locus_tag="PWX02_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_063101322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HDL1728068.1"
                     /translation="MEANHQRNDLGLVALTMLAQYHNISLNPEEIKHKFDLDGKGLSL
                     TSWLLAAKSLALKAKHIKKEISRLHLVNLPALVWQDNGKHFLLVKVDTDNNRYLTYNL
                     EQDAPQILSQDEFEACYQGQLILVTSRASVVGQLAKFDFTWFIPAVIKYRKIFLETLI
                     VSIFLQIFALITPLFFQVVMDKVLVHRGFSTLNIITVALAIVIIFEIVLSGLRTYVFS
                     HSTSRIDVELGAKLFRHLLSLPISYFENRRVGDTVARVRELDQIRNFLTGQALTSVLD
                     LLFSFIFFAVMWYYSPKLTLVILGSLPCYILWSIFISPILRRRLDDKFARSADNQAFL
                     VESVTAINMIKAMAVAPQMTDTWDKQLASYVSSSFRVTVLATIGQQGVQLIQKTVMVI
                     NLWLGAHLVISGDLSIGQLIAFNMLSGQVIAPVIRLAQLWQDFQQVGISVTRLGDVLN
                     SPTEQYQGKLSLPEIKGYISFKNIRFRYKPDAPTILNNVNLEIRQGEVIGIVGRSGSG
                     KSTLTKLLQRFYIPENGQVLIDGHDLALADPNWLRRQIGVVLQDNVLLNRSIRENIAL
                     SDPGMPMERVIYAAKLAGAHDFISELREGYNTIVGEQGAGLSGGQRQRIAIARALVNN
                     PKILIFDEATSALDYESEHIIMQNMQKICQGRTVILIAHRLSTVKNADRIIVMEKGEI
                     VEQGKHHELLQNSNGLYSYLHQLQLN"
     gene            5744..7180
                     /locus_tag="PWX02_000004"
     CDS             5744..7180
                     /locus_tag="PWX02_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_140441352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HDL1728069.1"
                     /translation="MKIWLSGIYEFFLRYKNIWAEVWKIRKELDHPNRKKDESEFLPA
                     HLELIETPVSKKPRLIAYLIMLFLVVAIVLASVSKVEIVATAPGKLTFSGRSKEIKPI
                     ENAIVQEIFVKDGQFVEKGQLLVSLTALGSDADIKKTMASLSLAKLENYRYQTLLTAI
                     EKESLPVIDLSRTEFKDSSEEDRLRIKHLIEEQYTTWQKQKTQKTLAYKRKEAEKQTI
                     FAYVRKYEGATRIEQEKLKDFKALYKQKSLSKHELLAQENKLIEAQNELAVYRSKLNE
                     LENDLLNVKEELELITQFFKSDVLEKLKQHIENERQLRLELEKNNQRRQASMIRAPVS
                     GTVQQLKIHTIGGVVTTAETLMIIVPEDDVLEATALVPNKDIGFVAAGQEVIIKVETF
                     PYTRYGYLTGRIKHISPDAIEQPNVGLVFNATIAIDRKNLTSPDGRKIDLSSGMTITA
                     EIKTGERSVMSYLLSPLEESVTESLRER"
     gene            7375..8166
                     /locus_tag="PWX02_000005"
     CDS             7375..8166
                     /locus_tag="PWX02_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1728070.1"
                     /translation="MSLSITNLSLGYRKNQQTKTILHDVTFNVESGTLTSILGPSGVG
                     KSSLLRVISGLDKPLSGKVALFGEKINQPHPDVGFVFQSATLLPWLSVRNNVAFGLDF
                     QCRLPLSKADIQHRVESALEEVGLREAIDLMPHELSGGMAQRVNLARALARQPKVILL
                     DEPFSALDEVIRSQMQTLLHDIVRHHNAAAIMVTHDIDEALLVSDDVLLLGGDTAHIV
                     GKWHLSAPFPRTDLLSLNPIRVEILHTLRQAQQRAVQEQTVDFVI"
     gene            8177..9343
                     /locus_tag="PWX02_000006"
     CDS             8177..9343
                     /locus_tag="PWX02_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1728071.1"
                     /translation="MDQQRRDFMKLLSLFSLAGSLPLIQACEQVQKNAENRPLVIGYL
                     PITDATPLLVAHAKGLFEKHGVQVEKPVMFRSWAQLVEAFLSGNVNVVHLLSPMSLWA
                     KYGANAPVKAVMWNHLAGSALTVRPEINSIAELSGKTVAIPFWYSIHNIVLQRLLREN
                     GLKITEKEPNSGEVRLSVMAPSDMVAALASEAISGFIVAEPFNALAEAKGVGKVLRFS
                     GDVWKDHACCLTLMHEYDIQHRPDWVQKVVNALTEAQVYTLENRAETAKLLARGSGYT
                     PHDQKVLEKVLAPTQAQWDKYIETGAIQNPQWHQQRIGFQPYPFDSYMEELVKMLKQT
                     HIAGNNAFLQQLDPVEAAKAINEPTFVRKAIEQGGWVETFNLQAGWTRTEQISV"
     gene            9360..10103
                     /locus_tag="PWX02_000007"
     CDS             9360..10103
                     /locus_tag="PWX02_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1728072.1"
                     /translation="MKQKYIYGLAGLLLLLLGWQLGTALLSQRLPMASMLAPAKTWES
                     FTWLISSGEIFPHLWASLQRIAVGLLAALVIGVPLGLLLGFSKHLEMLCTPSFQLLRM
                     ISPLSWMPIIVMLLGVGDLPIYFLLAFATVWSLIISTTSGVKSIDPQWLQLGRVMSAT
                     RKELLCKIVVPAITGHVLTGLRVAVGIAWVVLVPCEMLGVNQGLGYFILDTRDRLAYS
                     ELMAVIIIIGLIGWALDSLIRLALAKGGR"
     gene            10258..11316
                     /locus_tag="PWX02_000008"
     CDS             10258..11316
                     /locus_tag="PWX02_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HDL1728073.1"
                     /translation="MSVLSSSFIQWLQQNAENLDQSNERADLLIPQIAALGLYKIGVP
                     ETLGGSGGTLVDAIKAVTELGNHSLAAAFVSWGHRTFIEHIIASKNPIPRQSYLADLL
                     NGNLTAGTGLSNAMKFLSGIEELNVNIREENGKRYLNGRLPWVTNLRSYRFVASFVAG
                     FQDERKPLVIAVPSTAEGLTRTKELEFMSLQGTNTTALVFDNVELKEEWILSDNAPEF
                     LATTRPAFLGLQFGLPFGLVEKSLSEIEKSLSFRAILTSEWQSQVDALNDIKNRLFNG
                     LNEEGYFVRNPKELFQARIDIVNVVAQTILLELQASGGRGYLKNDTSGFSRRWKEAAF
                     LPVVTPSAVQLRMVLANS"
     gene            complement(11427..11969)
                     /locus_tag="PWX02_000009"
     CDS             complement(11427..11969)
                     /locus_tag="PWX02_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL1728074.1"
                     /translation="MSQFQIHTIESAPETAQEALKAVQQANGFIPNLIGVLANAPTAL
                     ETYRTVGGINSRNSLTAEEREVVQITAAVVNGCGFCVAGHTKIALKALKMPEEVVNAL
                     RATARIDDNKLDTLARFTLAVMLQKAKLTEAQLNEFFAAGYNQQNAIDVILGVSLATL
                     CNYVNNIAETPINPELQPFA"
     gene            complement(12121..13632)
                     /gene="glpK"
                     /locus_tag="PWX02_000010"
     CDS             complement(12121..13632)
                     /gene="glpK"
                     /locus_tag="PWX02_000010"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDL1728075.1"
                     /translation="MEKQYIIALDQGTTSSRAVLLDKNANMVEVAQREFTQIYPQAGW
                     VEHNPMEIWSSQSSTLNEVVAKAGIKPDSIAAIGITNQRETTIVWEKETGKPVYNAIV
                     WQCRRTSDITDKLKADGHEEYIRKTTGLVVDPYFSGTKVKWILDNVEGAREKAERGEL
                     LFGTVDTWLVWKLTQGRVHVTDYTNASRTMLFNIHTKQWDDKMLELLNIPRSMLPEVK
                     NSSEVYGQTNIGGQGGVRIPVAGIAGDQQAALYGHLCVKEGQAKNTYGTGCFMLMHTG
                     NTAIQSENGLLTTIACNAKGEPEYALEGSVFIAGASIQWLRDELKIVHDSFDSEYFAT
                     KEDDSNGVYVVPAFTGLGAPYWDPYARGSIFGLTRGANRNHIVRATLESIAYQTRDVL
                     EAMQSDSKAKLQALRVDGGATANNFLMQFQADILDTKVERPKVKEVTALGAAYLAGIA
                     VGFWKDLEELKDKAEIERTFTPDGDQEKRAKRYKGWKKAVRRSLEWAKEDAEE"
     gene            complement(13636..14421)
                     /locus_tag="PWX02_000011"
     CDS             complement(13636..14421)
                     /locus_tag="PWX02_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL1728076.1"
                     /translation="MSKSLRNACIGEFIGTGFILFFGAGCVAAAQVAGANFGLWEISI
                     VWGLGVSMAIYISAGISGAHLNPAVTIALAAFYGFEKHKILPYVIAQVAGAFCSVALI
                     YFMYSDLFTAAEAAQGITRGETVGFAGVFSTYPNPNITLVTAFIVEFVITVVLMSTIL
                     AIGDDKNGLPNKALAALLIGLLIAVIGGATGPLTGFAMNPARDFGPKLFAYLAGWGEI
                     ALTGGKEIPYFIIPIVAPICGALFGAWGYKNLIHNNLPANTQE"
     gene            14634..14993
                     /locus_tag="PWX02_000012"
     CDS             14634..14993
                     /locus_tag="PWX02_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HDL1728077.1"
                     /translation="MISIGAVLGAMCRWQLAVWLNPILSQFAFGTLAANWLGCLLIGI
                     AMGFNLGESQRLLFISGFLGSFTTFSSFSLELTEKLLAEKWGQFVTVLSLHLIGGLLL
                     TLIGVMLARAVTSGRFC"
     gene            complement(15041..16363)
                     /locus_tag="PWX02_000013"
     CDS             complement(15041..16363)
                     /locus_tag="PWX02_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDL1728078.1"
                     /translation="MSAMFIIQFAIVLLCILVGARVGGIGLGVFGGLGLAILSFGFGL
                     KPAGLPIDVMFMIMAVVSAAAAMQAAGGLDYMIKIATRILRKNPKYITFIAPLVTWTF
                     TVLAGTGHVAYSVLPVIAEVSRHNGIRPERPLSMAVIASQFAIVASPIAAAVVAVVAF
                     LEPQGVTLGNVLSVTIPATLLGLGLACVFVNKMGKELKDDPNYQRLLQDPEYVKENHI
                     TTNPTELPLKPTAKLSVGLFLFGALLVVLMGAIPALRPVFDGKPMGMAHTIEIVMLSI
                     GALIILTCKPDGNEITKGSVFHAGMRAVIAIFGIAWLGDTLMQGHMDEVKSMVSGLVE
                     TAPWAFAFALFVLSVLVNSQGATVATLFPVAIAIGIPAPVLIGVFVAVNGYFFIPNYG
                     PIIASIDFDTTGTTRIGKYIFNHSFMLPGLLSMIFSIGFGLLLSNILL"
     gene            16512..17336
                     /gene="dapF"
                     /locus_tag="PWX02_000014"
     CDS             16512..17336
                     /gene="dapF"
                     /locus_tag="PWX02_000014"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDL1728079.1"
                     /translation="MQFSKMHGLGNDFMVVDGVTQNVYLTEEMIRTLSDRHRGIGFDQ
                     LLLVEPPYDPELDFHYRIFNADGSEVSQCGNGARCFARFVTLKGLTNKKDIHVSTAKG
                     KMVLSLQEDGKVRVNMGEPIWEPSQVPFTANKFEKNYILRTELQTVLCGVVSMGNPHC
                     VLQVDDVKTAPVNELGPLLESHERFPERANISFMQVVNRNHVKLRVYERGAGETQACG
                     SGACGTVAVGIMQGLLDNKVQVDLPGGTLIIEWQGVGHPLYMTGDATHIYDGFIKL"
     gene            complement(17507..18049)
                     /locus_tag="PWX02_000015"
     CDS             complement(17507..18049)
                     /locus_tag="PWX02_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase family protein"
                     /protein_id="HDL1728080.1"
                     /translation="MAQVYIVHGYTANADKHWFPWLEQELAKCGVSCERLNMPDSAKP
                     SLDGWLNHLAQNVELTDQTVFVGHSLGCIAILNFLAKNYAKIKGAVFVSGFYQPVENL
                     PELSAFTNYYAILPSVSSFPSYVVSALDDKVVNHQYSDALAQHLHADYIRLNQGGHFL
                     DREGVTELPVVLELVKKLLE"
     gene            18173..18496
                     /locus_tag="PWX02_000016"
     CDS             18173..18496
                     /locus_tag="PWX02_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
                     /protein_id="HDL1728081.1"
                     /translation="MNVWILLAISICLEIAATNLLKLSNGFTKAIPTIGSLALYGLSF
                     YFLSIIFRTLPVGIVYAIWSGVGIVLTALVAYFAFGQKIDLAGLIGISLIIAGVLVIN
                     LFSKV"
     gene            complement(18559..20004)
                     /gene="xylB"
                     /locus_tag="PWX02_000017"
     CDS             complement(18559..20004)
                     /gene="xylB"
                     /locus_tag="PWX02_000017"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDL1728082.1"
                     /translation="MYIGIDLGTSGVKVVLLDENQHIIAITQKSLPIFRPHPLWSEQN
                     PQDWWNATNEAVLELSATQDLQAVKAISLTGQMHGATLLDNADNVLSPAILWNDGRSF
                     MECEELEQLVPNSREITGNLMMPGFTAPKLRWVDKHQPDIAEKVSKVLLPKDYLRLLM
                     SGEYASDMSDASGTMWLDVGKRNWNKSLLNACGLDIENMPKLFEGNQITGYLRPTLAE
                     QWKMKSVPIVAGGGDNAAGAIGIGLYQTGQAMLSLGTSGVYFVVSDKFQANPQKAVHS
                     FCHALPDRWHLMSVILSAASAVDWAVKSLGLKDVSTLFQQVEASQTASDAIFLPYLSG
                     ERTPHNDPYAKGVFWGLSHNDNQITMAKAVIEGVSFALAEGIEVLHETGVVADNIALI
                     GGGAKSAYWRQLLADISGKTFEYRTSGDVGPALGAAKLAQIALNPNQDIATFCQPLPL
                     EQVYQPTPARYAEYQQKRQKFTELYRRLKGL"
     gene            complement(20157..21476)
                     /gene="xylA"
                     /locus_tag="PWX02_000018"
     CDS             complement(20157..21476)
                     /gene="xylA"
                     /locus_tag="PWX02_000018"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDL1728083.1"
                     /translation="MSNYFDKISKVQYEGANSTNPFAFKHYNPDEVVLGKTMAEHLRL
                     AVCYWHTFCWTGNDMFGVGSLDRSWQKTGDLLQGAKQKAEIAFEFFQKLGIPYYCFHD
                     VDVAPEGNNFKEYQHNFNTIVDILEQKQAETGVKLLWGTANCFTNPRYMSGASTNPNP
                     EVFAWVAAQVFTAMNATKRLGGENYVLWGGREGYETLLNTDLKREREQIGRFMQLVVE
                     HKHKIGFNGTLLIEPKPQEPTKHQYDYDVATVYGFLKQFGLEKEIKVNIEANHATLAG
                     HTFQHEIATATALDIFGSIDANRGDPQLGWDTDQFPNSVEENTLVMYEILKAGGFTTG
                     GFNFDAKIRRQSTDPYDLFHAHIGAIDVLALSLKRAAKMLEDQALQKVVDNRYAGWNQ
                     DLGLQILNGKASLEDLAKIVETQGLDPKPVSGQQEYLENLVNSYIYR"
     gene            21718..22719
                     /gene="xylF"
                     /locus_tag="PWX02_000019"
     CDS             21718..22719
                     /gene="xylF"
                     /locus_tag="PWX02_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005576719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL1728084.1"
                     /translation="MKLKSKLLAVATATLMVLSNASVAKDLKIGMSIDDLRLERWQKD
                     RDIFVNKAESLGAKVFVQSANGDATAQISQIENMLNKDIDVLVIIPFNGEVLSNVIAE
                     AKKEGVKVLAYDRLINNADVDFYVSFDNEKVGELQAKSIIGQKPEGNYFLMGGSPVDN
                     NAKLFRKGQMKVLQPLIDSGKIKVVGDQWVDSWLAEKALQIMENALTANKNNIDAVVA
                     SNDATAGGAIQALSAQGLSGKVAISGQDADLAAIKRIIAGTQTMTVYKPITNLADKAA
                     EIAVALGKDEKVEMNAQLNNGMKEVPAYLLEPVVVTKENIDDTVIKDGFHTKDAVYK"
     gene            22786..24300
                     /gene="xylG"
                     /locus_tag="PWX02_000020"
     CDS             22786..24300
                     /gene="xylG"
                     /locus_tag="PWX02_000020"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HDL1728085.1"
                     /translation="MAKLLEMKNITKKFGEVVALNNISISLETGEILSLCGENGSGKS
                     TLMKVLCGIYPAGDYEGEIYFSGEKLIARNIKDTEEKGISIIHQELTLVKNMSILENM
                     FLGNEITKSGITDDNEMYLRCKTLLEQVQLDIDPNTKVGELGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEKETEILLSLIKDLQAHNIACVYISHKLNEVKAISDKICVIRDG
                     EHIGTRSAEGMSEDDIITMMVGREITSLYPHEPHNIGEEVLRVENLTAWHPTNTHIKR
                     VDNANFVLRQGEILGIAGLVGSGRTEMAQCIFGSYQGKYQGDIFLHKQPIKIKNCAQA
                     IENHIVMVPEDRKKHGIIPIMGVGKNITLSSLPQFCFGKKVINEPLEETIINQSIAKL
                     KVKTSSPELAIGRLSGGNQQKAILAKCLLLNPKILILDEPTRGIDVGAKYEIYKLINQ
                     LAKEGMAIIVISSELPEVLGISDRVLVMHQGKIKADLINQDLTQEKVMEAALKE"
     gene            24304..25431
                     /locus_tag="PWX02_000021"
     CDS             24304..25431
                     /locus_tag="PWX02_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1728086.1"
                     /translation="MNKLKSINLQVYIMLIAIVVIMSFFSIATDGAYLSARNISNLLR
                     QTSITGILAIGMVFVIISAEIDLSVGSLMGLLGGFAAISNVWWGWPLSLTIIVTLILG
                     VMLGAWSGWWVAYQKVPSFIVTLAGMLAFRGVLVGLTNGTTVSPISNEMTTIGQGYLP
                     DMVGMILGTIGIFCFIVWGSYQRKARQKLNLTVPSMSKDTLKYGLVAIIVLGAIYLLN
                     DYRGVPFPVLVLVILAIAGTFLARKTAFGRHIYAIGGNIDAARLSGISVEKVKLAIFA
                     LNGLLVAIAGLILSSRLGAGAPSAGQNAELDAIAACVIGGASLAGGIGSIYGVVIGAF
                     IIALLDNGMSMLDVPTFWQYIVKGGILLLAVWVDTISKKKA"
     gene            complement(25580..27445)
                     /locus_tag="PWX02_000022"
     CDS             complement(25580..27445)
                     /locus_tag="PWX02_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDL1728087.1"
                     /translation="MQSLLSFVPDFLKQELFWVMALLVFAIWQFIQNKLRMDVVALIV
                     MLFFSLTNILSVKEVLAGLSDPNVVLIALLFIVGEGLVRTGVANQVSDWLMRVSGASE
                     TKVLVLLMLCIAGLGSFMSSTGIVAIFIPVVLAICSGMGISPRRLMMPLSVAGLISGM
                     MTLIATPPNLVAHSELVKSGFEGFNFFSFTPIGLIILVMGIGYMLIARRWLDNGEQSN
                     THSQAQKFSMSQLIEEYQLKERAKMVLVEADSDCVGKSIGQLNLRIEQGLNIIAIQRT
                     KRFRKITVSASVNEIFQPKDILLLDTALDDETYQQRCEQLKLRPIELKGEYFSSQYRS
                     VGMAEISVMPEAECIGKTIRELKFRSRYHLSIVGLKRGDEIIQEELLDTPLKFGDILL
                     VMGVWQQIHRMDDDHRDFFLLRDPAESKNAPPALSQAPHALFSVLTMVLLMITGIVPN
                     VMAALIACLMLGKFRCVDMKSAYDSIHMPSIVLIVGMMPFSIALQKTGGVDLTVRLLL
                     DAMQGLDVHFVLIVLFVVTAIISAFISNTATAILVMPIAIAIANQLGYSPAPFVMTVA
                     VSASAAFMTPVSSPVNTMVLAPAGYKFIDFVKVGVPFTVLVMLVSVFVIPMLFPL"
     gene            complement(27585..28100)
                     /locus_tag="PWX02_000023"
     CDS             complement(27585..28100)
                     /locus_tag="PWX02_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltR family transcriptional regulator"
                     /protein_id="HDL1728088.1"
                     /translation="MMDYIDKLSEETTLRGFLSIANQRFAKAVEQLIERVFRKTDFAL
                     KSVVDSLFEHQGPLADIEVRLKVLLGLGVISPKVFEDISLFLEVKQHLNEEEDEFAFS
                     HPAVVKFAKDLHHIDFLPINELLNMKASAANKDSMLFQMQQMRLEKMVRSSLILAVTE
                     IDEQLNVESPL"
     gene            complement(28112..29251)
                     /locus_tag="PWX02_000024"
     CDS             complement(28112..29251)
                     /locus_tag="PWX02_000024"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005557909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDL1728089.1"
                     /translation="MNALHFGAGNIGRGFIGKLLADARIFVTFADINQTQIDQINQNK
                     QYGVKIVGDDSRVEIVKNIAAINSKDENAVIEQVKNTDLITTAVGPNVLGFIAPLFAK
                     ALVARVESGNTQPLNIIACENMVRGTTFFKGKIFEHLTAEQQAEIEKVVGFVDSAVDR
                     IVPPAEPNPADPLEVTVEEFSEWIVDQTQFKGDIPNIKGMELTDNLMAFVERKLFTLN
                     TGHLICAYLGKQAGVKWIKEAIAIEEIKTQVKATMEESGAVLIKRYGFDPQAHSAYIE
                     KILKRFANPYLNDDVNRVGREPIRKLSENDRLIKPLRGTLEYGLPYQNLVKGVVMALQ
                     YRNEEDPQAVELAEFIANHGVAAAVEKYTGLTDQAVIAQVVELYQ"
     gene            complement(29355..31214)
                     /locus_tag="PWX02_000025"
     CDS             complement(29355..31214)
                     /locus_tag="PWX02_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDL1728090.1"
                     /translation="MSNLKVKVQGFGRFLSNMVMPNIGAFIAWGFITALFIPTGWLPN
                     ETFAKLVGPMITYLLPLLIGYSGGKLVGGERGAVVGAITTMGIIVGSEIPMFLGAMIV
                     GPLGGWAIKTFDKAIDGKVKSGFEMLVNNFSVGIIGMILAMLSFAFIGGIVTQISAAL
                     GAGVGALVEAGLLPVTSIIVEPAKILFLNNAINHGVFTPLGTAQAQEVGKSILFLIEA
                     NPGPGFGILLAYMFFGRGTAKQTAGSASIIHFLGGIHEIYFPYVLMSPRLILAVIAGG
                     ATGVLTNSLLGGGLIAPASPGSIFAILAMTAPGAHFAVVASVILSCAVTFAIASILLK
                     TQKGEASLEDAQAQTQAMKAESKGVVNGNINKIYVACDAGMGSSAMGASMLRKKVQAA
                     GLNIEVQNLAINDLPQDAQLVITHKDLTARATQRVATAQHLSLTNFLDSAFYDSLVAK
                     LANHQPQQAVQNTQNFANPVEEPQAEGLKLQVAQVFLGLQANSKEEAIRFAGEQLVKA
                     GFVQPSYVDAMFAREQMVSTYLGEGLAVPHGTSDAKDSVLKTGIVVCQYPNGVRFTDE
                     EDGVAKLVVGIAAKGNDHIQILSAITNALDDEQAMKTLTSTNDVEKVLALLSK"
     gene            31516..32069
                     /locus_tag="PWX02_000026"
                     /pseudo
     CDS             31516..32069
                     /locus_tag="PWX02_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005752768.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(32113..33048)
                     /locus_tag="PWX02_000027"
     CDS             complement(32113..33048)
                     /locus_tag="PWX02_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HDL1728091.1"
                     /translation="MNHLDSAKETLNLYAQQIITLNHRLSEEFNEVVNMILACQGRVV
                     VGGIGKSGLVGKKMVATFASTGTPSFFLHPTEAFHGDLGMLKPIDMVILISNSGETDD
                     VNKLIPSLKNFGNKIIAMTGNPYSTLGRNADVILNIGVEREACLNNLAPTSSTLVTMA
                     LGDALAIALMKARDFRPEDFARYHPGGSLGRKLLNRVRDVMVRKVPTASLDTTFTECL
                     SVMNEGRMGVAVIMQDDKLEGIITDGDIRRTLAKFGAESLNKTADEIMTRNPKTINDT
                     EFLAKAEDLMKELKIHSLIAVNDEGKVTGLMEFSS"
     gene            complement(33059..33856)
                     /locus_tag="PWX02_000028"
     CDS             complement(33059..33856)
                     /locus_tag="PWX02_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor"
                     /protein_id="HDL1728092.1"
                     /translation="MKPFERRQQILAYLAQHGRTEVDVLADYFKLTGATIRKDLTSLE
                     EDKKVLRTYGSVVLIDEEILEREEKINKEDKDDKPLAQKTGINLQQKQAIGQKAAELI
                     NEGNSIIFDAGSTVLQMIPHLKAFDNLSLMTNSLTIFNEILKLNKSYNLLISGGTFRE
                     KSASFHGYFAESAFIGSNFDILFIGTDGLDLDVGLTTFNEVYKVSSLMCDAANKIVLL
                     ADSSKFGRKSPNIVCGLEKIHTIITDEGLSQEMREQIKAKGIELLVV"
     gene            complement(33899..34546)
                     /gene="hxpB"
                     /locus_tag="PWX02_000029"
     CDS             complement(33899..34546)
                     /gene="hxpB"
                     /locus_tag="PWX02_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HDL1728093.1"
                     /translation="MQINSVIFDMDGVMIDSEPQWAEAQIAVLADLGINITEQDCEQL
                     TRGKRIDELSDTWIKKFQLSVSNQALSEAILAHGCAAIEQHGKALTGLYELLDFLKSN
                     QIKLAVATSSPPVVIKAVFDRLKLWDYFPIRCSAMDEEYGKPHPAVYLSAVKKLGIEK
                     QECIVIEDSVTGLIAAKAANLTTFLVNTDYQNEKFSIADKRIASLLDVIEKLKQH"
     gene            complement(34609..34989)
                     /gene="gutM"
                     /locus_tag="PWX02_000030"
     CDS             complement(34609..34989)
                     /gene="gutM"
                     /locus_tag="PWX02_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDL1728094.1"
                     /translation="MDSITLLIILAMIMWLLQAVLGWLQIKAFNQAFMEISKKGKVTV
                     GRNSGRFNPKSVIVLAFDDEKKVVDSLCMKGFSVFARPQKLASVIGLSLDQIVPEKIF
                     PSDKRSQFALKVAISSGYSCSDQK"
     gene            complement(35158..35946)
                     /gene="srlD"
                     /locus_tag="PWX02_000031"
     CDS             complement(35158..35946)
                     /gene="srlD"
                     /locus_tag="PWX02_000031"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDL1728095.1"
                     /translation="MNNEKKVAIVVGGGQTLGAFLSEGLADAGYRVVVADSNGENAQK
                     VAADINAKQGEGNAIGVQVDAADEPSVEALAKATDTVFGRADVLVYSAGTAKASPIWD
                     FNLNDFDLSVKVNLTGYFLTAKHFSRLMIRDGIKGRIIQINSKSGKVGSKYNSGYSAA
                     KFGGVGLTQSLALDLADKGITVHSLMLGNLLKSPMFQSLIPQYAAKLGIPESEVEQVY
                     IDKVPLKRGCDYQDVLNVLKFYASEEAAYCTGQSINITGGQVMF"
     gene            complement(35994..36392)
                     /gene="srlB"
                     /locus_tag="PWX02_000032"
                     /gene_synonym="gutB"
     CDS             complement(35994..36392)
                     /gene="srlB"
                     /locus_tag="PWX02_000032"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL1728096.1"
                     /translation="MHRLSKRENIYMTIIYQTTFTKIGNFAQESLTDDMLITFKQGAP
                     ADLEDYCFIHNPSELSSPLEVGDIAEFDGVAYPITAVGSVASENLSALGHITFRFDGA
                     NDAEFPGSVHVIGTPPQGLTENSTLIIKRD"
     gene            complement(36437..37438)
                     /locus_tag="PWX02_000033"
     CDS             complement(36437..37438)
                     /locus_tag="PWX02_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDL1728097.1"
                     /translation="MSNKAIYIEKGNGGWGGPLTLPVVEGKKVLYMTGGTRPAIVNKL
                     VELTGWEAVDGFKEGEPLAEEIGIAIIDCGGVLRCGLYPKRRIPTINIHSTGKSGPLA
                     EFIVEDIYVSAVKENNIRLIDADGSVPVATEQAKPTSSTTATAKEYDSSKKITEQSDG
                     LLAKIGMGMGSVAALFFQAGRDTIDTVLKTILPFMAFVSALIGIIIASGLGDVIAHGL
                     TPLANSPIGLVTLALICSFPLLSPFLGPGTVIAQVIGVLVGTQIGLGNIPPHLALPAL
                     FAINAQAACDFIPVGLSMAEAKQDTVRVGVPSVLVSRFLTGAPTVLIAWAVSAFIYQ"
     gene            complement(37469..38023)
                     /gene="srlA"
                     /locus_tag="PWX02_000034"
     CDS             complement(37469..38023)
                     /gene="srlA"
                     /locus_tag="PWX02_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725059.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDL1728098.1"
                     /translation="MIEAITHGAEWFIGLFQKGGETLVGMINGILPLLISLLVVMNAL
                     IKFVGQDRIERLAQRSAGNPISRYLILPVIGTFVFCNPMTLSLGRFLPERYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLGLPLGPLAISYLLVGMFTNFFRGWI
                     TDFTTAFFEKRMGITLDREVHLSH"
     gene            complement(38260..38568)
                     /locus_tag="PWX02_000035"
     CDS             complement(38260..38568)
                     /locus_tag="PWX02_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDL1728099.1"
                     /translation="MKKYIATMLLAFGLVHSSYAQEKTAQIETKQAEKTVVLHIPEMY
                     CQLCVYLVNKELRAVEGVISTKASMKERKATVVVKESVDDQALIQAVGNLKYTAKVIE
                     "
     gene            complement(38570..38938)
                     /locus_tag="PWX02_000036"
     CDS             complement(38570..38938)
                     /locus_tag="PWX02_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728100.1"
                     /translation="MNSSLKSYNHKFITMCVTAIVSAVTSTFCCIGPLVYLVFGISSP
                     WLMELNQLEFLQLPMLLISLAAFGYGFWLLNFSNKIICTKYFSRRTLVILYWVVSVVV
                     MFFLTYPYVLPYILEMWRGE"
     gene            complement(38913..40019)
                     /locus_tag="PWX02_000037"
     CDS             complement(38913..40019)
                     /locus_tag="PWX02_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HDL1728101.1"
                     /translation="MNKLIASLVASSCATAFAAPVQQNNFHTTTHTYEFTQSYDLVVP
                     QGSKGTTNLWIPLPSDTDYQTVKSIEFEGSYKNAYITENNAYGAKTLFATWDTDAPKR
                     DLKVKMVIQTQDREPMAQGALANYQMPEKITYSVDVLEYLKPTAHIKTDGIVKEYADK
                     IVGNEQNPLKKAELIHKWIVDNMERDNSVLGCGDGDVEKILTTKVLKGKCTDINSVFV
                     ALARASNIPAREVFGIRLGQAVKMGQYSKTAFGSSKDGVANESGGQHCRAEFYLAGFG
                     WVPVDSADVAKMRLAEKKDVDDADVQAVSKYLFGNWEMNWVGFNHGRDFDLYPQPELT
                     PLNNFGYPYAEVGGDPLNSFEAKEFGYEFISKEL"
     gene            complement(40259..41302)
                     /locus_tag="PWX02_000038"
     CDS             complement(40259..41302)
                     /locus_tag="PWX02_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL1728102.1"
                     /translation="MQPINIAIAGAGFSSRVFHIPFLKQDARFNIVKVYERTSNNAAE
                     WLPNAETVRDFNALLTPEVDLVLITTPNQTHYAMVKESLLAGKHVLVEKPLVATVAEA
                     EELAELAKRQGKVLYVYQNRRWDSHIATAKSLMEQNLLGELVDCEIRFDRYAKEKNVK
                     VWKESGELGTGLVYDLGVHLIDQALYLFGKPEAVYADVRYQHEGALVDDNFDIQLYYA
                     NGFKVVLKASRYAREPSPTFVLHGKLGSYLKQTPDNQEDLLKNGVQPIGKDWNIEEKE
                     NWGILHTEVEGNVVRQPYRNAEGSYQNLFDDLYQAIAHNAEPIIKLEDVIFVLKVIES
                     VFESAKLGQKIYL"
     gene            complement(41406..41690)
                     /locus_tag="PWX02_000039"
     CDS             complement(41406..41690)
                     /locus_tag="PWX02_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728103.1"
                     /translation="MRYWLISLSILLSGCVVPVTPTGQAGIPMPIIIGVSDPLDSKEV
                     HICTLKPFTQEYRSEHSSRGRAKLNVQKQCRANHSEMFCEEKDIVCKTYD"
     gene            complement(41720..41905)
                     /locus_tag="PWX02_000040"
     CDS             complement(41720..41905)
                     /locus_tag="PWX02_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HDL1728104.1"
                     /translation="MKCRQATELISQSCDEKLRLQQHMRLQLHLLVCPKCRNFNKNTK
                     KLGKILRAYCNDKDAPF"
     gene            complement(41902..>42338)
                     /locus_tag="PWX02_000041"
     CDS             complement(41902..>42338)
                     /locus_tag="PWX02_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL1728105.1"
                     /translation="SASFRREAALKTWIFAILKNKILDLLRTRKRHIPVSSLCEEEES
                     PSAFFDRHDHWLEHQDVTEWRGIAQSTYQKEFWAIFDICLNKLPAQQARVFMMREHLE
                     MSSAEICQECEISLSNLNVLLHRARLRLQECLSRNWFREESK"
BASE COUNT        12513 a         8906 c         8719 g        12200 t
ORIGIN      
        1 aacactcctt tttctcttct gattatataa aagacaaaaa atacaattta agctaaaaaa
       61 aacaacaaaa aatacgacaa taagatcgag taatgattat attatgctat aatttttgac
      121 ctaatttaga ataattatcg agtgcaaatt atgaatcaat cttattttaa cttactagga
      181 aacattactt ggctatggat gaactcctcc ctccacaaag aatggagctg tgaactacta
      241 gcacgcaatg tgattcctgc aattgaaaat gaacaatata tgctacttat agataacggt
      301 attccgatcg cttattgtag ttgggcagat ttaaaccttg agactgaggt gaaatatatt
      361 aaggatatta gttcattaac accggaggaa tggcagtctg gcgatagacg ttggattatt
      421 gattgggtag caccattcgg acattctcaa ttactttata aaaaaatgtg tcagaaatat
      481 gctgatacac tcgtcagatc tatacgcttc caaccaaatc aaaaatcagt aggaaaaatt
      541 gcttatttca aaggaggtaa attagataaa aaaacagcaa aaaaacgttt tgatacatat
      601 caagaagagc tggcaacagc acttaaaaat gaatttaatt ttattaaaaa atagaaggag
      661 acatccctta tgggtaataa acttactaat atttcaacaa atttaaaaag ttcctggttg
      721 actgctaaat ccggtttaaa tagaacagga caatcattag ctaaagcagg gcaatcttta
      781 aaaacagggg caaaaaaaat catcctctat atcccaaaag attaccaata tgataccgat
      841 aaaggtaatg gtttacagga tttagttaag gcagctgaag agcttggtat tgaagtgcaa
      901 aaagaagaga gcaatgatat tgcaaaagca caaaccagtt taggcacaat tcacaatgta
      961 ttgggtttaa ctgagcgtgg tattgttttg tcagcccctc agcttgataa attgcttcaa
     1021 aaaaccaaag taggtcaagc gattggctct acagaaaata ttacaaaagg ttttagtaat
     1081 gcaaaaacag tattatctgg aatccaatct attctaggtt ctgtcttagc cggtatggac
     1141 ttagatgaag cattacaaaa taacagcaat gagctaacac ttgctaaagc tggtttagag
     1201 ttaaccaact cgttgatcga aaatattgct aactcagtga aaacattaga cgcctttggt
     1261 gatcaaatta atcaacttgg ttcaaaactg caaaatgtca aaggattaag ctctcttggc
     1321 gaaaaactaa aaggtctgag tggctttgat aaaaccagcc ttggtttaga tattgtatcc
     1381 ggcttgctgt cgggagcaac ggctgcactt gtacttgcag acaaaaacgc ttcaacatca
     1441 agaaaagtgg gtgcaggttt tgaattagca aaccaagttg ttggtaatat taccaaagcc
     1501 gtttcttctt acattttagc ccaacgtgtt gcagccggtt tatcttcaac cggacctgtg
     1561 gcagccttaa ttgcttctac cgtttctctt gcgattagcc cattatcatt tgccggtatt
     1621 gccgataaat ttaatcacgc aaaaagttta gaaagctatg ctgaacgttt taaaaaatta
     1681 ggctatgacg gtgataattt attagcagaa tatcaacgtg gaacagggac tattgatgct
     1741 tcagtaacag caatcaatac agcattggct gctattgctg gtggtgtatc tgccgctgct
     1801 gccggctctg tggttgcttc accaattgcc ctacttgtat caggtattac cggtgtgatt
     1861 tccactattc tacagtactc taaacaagca atgtttgaac acgttgcaaa taaaattcac
     1921 aataaaattg tagaatggga aaaaaataat ccaggtaaga actactttga aaatggttac
     1981 gatgcccgtt atcttgcgaa tttacaagat aatatgaaat tcttactgaa cttaaacaaa
     2041 gagttacagg cagaacgtgt tattgcaatt actcagcagc aatgggataa caacattggt
     2101 gatttagccg gtatcagtcg tctaggtgaa aaagtactta gcgggaaagc ctatgttgat
     2161 gcctttgaag aaggtcaaca tcttaaagcg gataaattag tgcaattaga ttctgcgaaa
     2221 gggattattg atgttagtaa cactggagaa gcgaaaacac agcatatttt attccggaca
     2281 ccgttattaa ctccgggaac agaaaaacgc gagcgagtgc agacgggtaa atatgagtat
     2341 attactaaac ttcatattaa ccgagtagat agctggaaaa ttacagatgg tgcagcaagt
     2401 tctacctttg atttaactaa cgttgttcag cgtattggta ttgaattaga caatgctgga
     2461 aatgtaacta aaaccaaaga aacaaaaatt attgccaaac ttggtgaagg tgatgacaac
     2521 gtatttgttg gttctggtac gacggaaatt gatggcggtg aaggttacga ccgagttcac
     2581 tatagccgcg gaaactatgg tgctttaact attgatgcaa ccaaagagac cgagcaaggt
     2641 agttataccg taaatcgttt cgtagaaagc ggtaaagcat tacacgaagt gacttcaact
     2701 cacaccgcat tagtgggcaa ccgtgaagaa aaaattgaat atcgtcatag caataaccag
     2761 caccatgccg gttattacac caaagatacc ttgaaagctg ttgaagaaat tatcggtaca
     2821 tcacataacg atatttttaa aggtagtaag ttcaatgatg cctttaatgg tggtgatggt
     2881 gtcgatacta ttgacggtaa cgacggcaat gaccgcttat ttggtggtaa aggcgatgat
     2941 attatcgatg gtggaaatgg tgatgacttt atcgatggcg gtaaaggcaa tgacctatta
     3001 cacggcggta aaggtgatga tattttcgtt caccgtcaag gcgatggtaa tgattcaatt
     3061 actgaatcgg aaggtaatga taaattatca ttctctgact caaatctaaa agatttaacc
     3121 tttgagaaag taaatcacca ccttgtgatt actaatacca agcaggaaaa agtcaccatt
     3181 caaaactggt tccgtgaagc agagtttgca aaaacaattc aaaattatgt tgcaacaaga
     3241 gacgataaaa ttgaagagat tatcggtcaa aatggtgaac ggattacctc gaagcaagtg
     3301 gatgaactta tcgaaaaagg taacggtaaa atcgctcaga gtgaactgac aaaagttgtg
     3361 gataactatc aattactcaa atatagccga gatgcatcaa acagtttaga taagttaatc
     3421 tcatctgcaa gtgcatttac ctcgtctaat gattcgagaa atgtattagc gagcccaact
     3481 tcaatgttgg atccgagttt atcttctatt caatttgcta gagcagctta atttttaatg
     3541 attggcaact ctatattgtt tcgcacatta tggagttgcc gttttatttt ataaaaggag
     3601 acaatatgga agctaaccat caaaggaatg atcttggttt agttgccctc actatgttgg
     3661 cacaatacca taatatttcg cttaatccgg aagaaataaa acataaattt gatcttgacg
     3721 gaaaagggct ttctttaacc tcttggcttt tagccgcaaa atcgttagcg ttgaaagcga
     3781 aacacattaa aaaagagatt tctcgcttac acttggtgaa tttacctgca ttagtttggc
     3841 aagataacgg taaacatttt ttattggtaa aagtggatac cgataataac cgctatttaa
     3901 cttacaattt ggaacaagat gccccacaaa ttctgtcaca agacgaattt gaagcctgct
     3961 atcaagggca attaattttg gtcacatcta gagcttccgt agttggtcaa ctagcaaagt
     4021 tcgatttcac ctggtttatt ccggcggtga tcaaataccg aaaaatcttt ctagaaacct
     4081 tgattgtttc gatctttttg caaatttttg ccctaattac accgctattt ttccaagttg
     4141 ttatggataa agtactggtg catcgaggtt tttcaacctt gaatatcatt acggttgcct
     4201 tagctattgt gatcatcttt gaaattgtct taagcggttt acgcacctac gtgttttccc
     4261 atagcactag tcggattgac gtggaattag gcgctaaatt atttaggcat ttattatcgt
     4321 tacctatttc ttattttgag aacagacgtg ttggcgatac agttgcacgg gtcagagaat
     4381 tggatcaaat acgaaatttc cttaccggac aagcattaac ctcggtatta gatttattat
     4441 tctcttttat cttttttgcc gtaatgtggt attacagccc gaaattgacc ttagtcattc
     4501 tcggctcact accctgctat attttatggt caatttttat tagtccgatt ttaagacggc
     4561 gtttagacga taaatttgcc cgaagtgctg acaatcaagc attcttagtt gaatcggtaa
     4621 cagccatcaa tatgattaaa gcgatggcgg ttgctccaca aatgacggat acatgggata
     4681 aacagctggc aagctatgtt tcatcaagct tccgtgtcac cgtattagca accattgggc
     4741 aacaaggtgt acaacttatt caaaaaaccg ttatggtgat caacctttgg ctaggggcac
     4801 acttagttat ttcaggcgat cttagtattg ggcaattaat tgcctttaat atgctatcag
     4861 ggcaagtgat tgcaccggtg attcggctgg ctcagctctg gcaagatttc caacaagttg
     4921 ggatttccgt cactcgctta ggtgatgttt taaactctcc aaccgaacaa tatcaaggca
     4981 aattatcact accagaaata aaaggctata tctcatttaa aaatatccgc tttagatata
     5041 aaccagatgc accaactatt ttaaataatg tgaatttaga aattaggcaa ggagaagtga
     5101 ttgggattgt tggacgttcc ggttcaggca aaagtactct gactaaatta ctgcaacgtt
     5161 tttatattcc tgaaaatggg caggttttga ttgatggaca tgatctagcc ttagctgatc
     5221 caaactggct acgccgtcaa ataggtgtag tgctgcaaga taatgtgtta ttaaaccgca
     5281 gtatccgaga aaatattgcg ctatcagatc caggaatgcc aatggagcga gtaatttatg
     5341 cagcaaaatt agcaggggct cacgatttta tttcagaatt gcgtgaaggt tataacacca
     5401 ttgtgggtga acaaggagcg gggctttcag gcgggcaacg ccaacggatt gcgattgctc
     5461 gagctttggt aaacaacccg aaaatcctga tttttgatga ggcaaccagt gccctcgatt
     5521 acgaatctga gcatattatt atgcaaaata tgcaaaaaat atgccaaggc agaaccgtga
     5581 ttttgattgc acatcgttta tcgaccgtca aaaatgcgga tcgaattatt gtgatggaaa
     5641 agggggaaat tgttgagcaa ggcaagcacc acgaattact gcaaaacagt aacggacttt
     5701 attcctactt acaccaatta caacttaatt aagaaggaaa acaatgaaaa tatggcttag
     5761 tggtatttat gaatttttcc tacgctataa aaacatttgg gcagaagtat ggaaaattcg
     5821 taaagaatta gaccacccaa acagaaaaaa agacgaaagt gaatttttac cggcacattt
     5881 agaactgatt gaaaccccgg tttctaaaaa accacgtcta attgcttatt tgattatgct
     5941 atttttagtt gtggcaattg tgcttgccag tgtaagcaaa gttgaaattg tggcgactgc
     6001 tcccggtaaa ttaactttta gtggcagaag taaagaaatt aaaccgattg aaaacgccat
     6061 tgtacaagaa attttcgtta aagatgggca gtttgtggaa aaagggcaat tattagtcag
     6121 cttaactgca ttgggttctg atgcagatat caaaaagacc atggcttcac tttctttagc
     6181 taaactggag aactatcgct accaaacttt gcttactgcc attgaaaaag agtccttgcc
     6241 ggtgattgat ttatctagaa ccgaatttaa agattcatcg gaagaagatc gactacgtat
     6301 taaacactta attgaggagc aatacaccac ttggcaaaaa caaaaaacac agaaaacttt
     6361 agcgtataag cgtaaagagg ctgaaaaaca aacaatattt gcctatgtcc gtaaatatga
     6421 aggtgcaaca cgtattgaac aagaaaaatt aaaagacttt aaggcacttt ataaacagaa
     6481 gtctttatct aagcacgaac ttcttgcgca agaaaataaa ttaattgagg ctcagaatga
     6541 gctagctgtt tatcgctcaa aattaaatga attagaaaat gatctactca atgtaaaaga
     6601 agaacttgaa ttgatcacgc aattctttaa aagcgatgtg ttggaaaaat taaagcaaca
     6661 tattgaaaat gaacgccaac ttcggctcga gttagaaaaa aataatcaac gcagacaggc
     6721 ctcgatgatc agagcaccgg tttccggtac ggttcagcaa ctgaaaattc acactatagg
     6781 tggtgttgtt acgactgctg aaaccttgat gatcattgtg ccggaagacg atgtgttaga
     6841 ggccaccgct ctggttccaa acaaagatat cggctttgtt gcagcagggc aggaggtgat
     6901 tattaaagtg gaaactttcc cttatacacg ctatggttat ctaactggtc gaattaaaca
     6961 tattagcccg gatgcgattg aacaacctaa tgtaggctta gtttttaatg caactatagc
     7021 tatagatagg aagaatctaa catcgcctga tgggcgaaaa attgatttga gttcaggtat
     7081 gacaataact gctgaaatca aaaccggtga acggagtgta atgagttatt tactcagccc
     7141 attagaagaa tctgtcacag aaagtttaag ggaacgctaa tcgaaccaaa acaaagccat
     7201 aaaagccatt ttgagctttt atggctttat tttttagtcc acaagcggtc aaaaaagccc
     7261 aattttttac acttttataa caaattgttc taactaaaaa ttactaattc ttttctttta
     7321 taagacgaat cctctatttt catttcatta acattgaact agaaaagggg atttatgagc
     7381 ctaagcatta cgaatctttc tcttggctac cgcaaaaatc agcaaaccaa gaccattttg
     7441 cacgatgtca cttttaacgt agaaagcggc actttaacct cgattctcgg gccgagtggg
     7501 gtggggaagt cctcgctgct gcgggtgatc agcgggttag ataaaccctt aagcggaaag
     7561 gtggcgttat tcggggagaa aatcaatcag ccacaccccg atgtgggctt tgtgtttcaa
     7621 tcggcgacct tgttgccttg gctttcggtg cgaaataacg tggcattcgg tttagatttt
     7681 caatgccgct taccactcag caaagccgat attcagcaca gggtggaatc ggctttggag
     7741 gaagtcggct tgcgtgaggc aatcgatctt atgccgcatg aactttccgg cggtatggca
     7801 cagcgggtga atttggctcg ggcattggct cgtcagccga aagtgatttt gctcgatgaa
     7861 cccttttccg cccttgatga agtcattcgc agccaaatgc aaaccttatt acacgatatt
     7921 gtccgccacc ataatgcggc agcgattatg gtaacgcacg atattgatga agccttattg
     7981 gtttcagatg atgtgttatt acttggcgga gataccgccc atattgttgg aaaatggcat
     8041 ttatctgctc cattcccccg tactgattta ttgagtctga accctattcg agtcgagatt
     8101 ttacacacgc ttagacaggc tcagcagcga gcggtgcaag agcagacagt tgattttgtg
     8161 atttaaggag aaagtgatgg atcagcaacg tagagatttt atgaaactgc tcagcctgtt
     8221 ttcccttgca ggtagtttac cgttaattca agcctgcgaa caggtgcaaa aaaatgccga
     8281 aaatcgaccg cttgttatcg gctacttgcc gattaccgac gccacaccgc tgttagttgc
     8341 tcacgccaaa ggcttatttg aaaagcacgg tgtgcaggtg gaaaaaccgg tgatgtttcg
     8401 cagctgggct cagttggtgg aagccttttt aagtggcaat gtgaacgtgg tgcatctgct
     8461 ttcgcctatg agtttgtggg cgaaatatgg agcaaatgct ccggtgaaag cggtaatgtg
     8521 gaatcacttg gcaggttcgg ctttaacggt tcgccctgaa atcaacagta ttgccgaact
     8581 ctccggcaaa acggtagcaa ttccgttttg gtattcaatt cataatatcg ttttgcaacg
     8641 attattacga gaaaatggct tgaaaatcac cgaaaaagag ccgaacagcg gcgaagtgcg
     8701 attaagtgtg atggctccgt cagatatggt agcagccttg gcaagcgagg caatttcagg
     8761 ctttattgtg gcagagccgt tcaatgcgtt ggcggaagcc aaaggtgtgg gcaaagtgct
     8821 gcgttttagt ggcgatgtgt ggaaagatca cgcctgctgt ttaaccctaa tgcacgaata
     8881 tgatattcag catcgccccg actgggtgca aaaagtggta aatgccttaa ccgaagcaca
     8941 ggtttatacg ctggaaaatc gggcagaaac agcaaaattg ttggcacggg gcagcggcta
     9001 cacgccgcac gatcaaaaag tgctggaaaa ggtactggct ccaacccaag ctcagtggga
     9061 taaatatatc gaaacagggg caattcaaaa ccctcagtgg caccaacagc gaatcggttt
     9121 ccaaccgtat ccgttcgatt cctatatgga agagctggtc aaaatgctca aacagaccca
     9181 cattgccggc aataatgcgt ttttacaaca gcttgacccg gtagaggcag cgaaagcgat
     9241 taacgagccg acctttgtgc gaaaagcgat tgagcaaggc ggttgggttg aaacctttaa
     9301 tttacaagcc ggttggacaa gaaccgagca aattagtgta taagcgatag gaacgaacga
     9361 tgaaacagaa atatatttac ggcttagccg gtttattact gctactttta ggttggcagc
     9421 ttggcacagc cttgttaagc caacggttgc cgatggcatc aatgttagct ccggcaaaaa
     9481 cctgggaaag ttttacttgg ctgatttcct ccggcgaaat ttttccgcac ttatgggcga
     9541 gtttgcaacg cattgctgtc gggctactcg ctgcgttagt gattggtgta ccgctgggct
     9601 tattgctcgg cttctccaag caccttgaaa tgctttgcac gccgtcattc caactactac
     9661 gtatgatttc gccactttcg tggatgccga ttatcgtgat gttacttggt gtaggcgatt
     9721 tgccgattta ttttttactg gcgtttgcca ccgtttggtc gttgattatt tccaccacct
     9781 caggggtaaa aagcattgat ccacaatggt tacaactggg tagagtaatg tcggctaccc
     9841 gcaaagaact gctgtgcaaa attgttgtac cggctattac cggacacgtt ctcaccggtt
     9901 tacgtgtggc ggttgggatt gcgtgggtgg tgctggtacc gtgcgaaatg ctaggcgtga
     9961 atcaaggact cggctatttt attttagaca cccgagaccg attagcctat tccgaactga
    10021 tggcggtaat tataattatc ggcttaatcg gctgggcgtt ggatagctta atccgcttgg
    10081 ctttagcgaa aggcggcaga taaattgaca agcggtcgaa aaattgcatt tttttgcaaa
    10141 aaatcgaccg cttgtttatt ccaaaaaagc atattacctt aatttatttt atttctaggg
    10201 cataaaaaag cgatttaaga tagcgacaaa gtaaactaat tatacgaggt aaacacaatg
    10261 tcggttcttt catcttcttt tattcaatgg cttcagcaga acgccgaaaa tttagatcaa
    10321 tctaacgaac gagcggattt actcattcca caaattgccg cactagggct atataaaatc
    10381 ggcgtgccgg aaacgcttgg cggcagcggt ggcactttgg ttgatgcgat caaagccgtt
    10441 accgaactgg gtaatcactc gctggctgcg gcatttgttt cgtggggaca tcgtaccttt
    10501 atcgagcata ttatcgcctc aaaaaatcct attccccgcc aaagctattt ggcggatctg
    10561 ctaaatggca atttaaccgc aggaacaggt ttatcgaatg caatgaaatt tctctccggc
    10621 attgaagagt tgaatgtgaa tattcgtgaa gaaaacggta aacgctattt aaatggccgc
    10681 ctgccttggg taaccaattt acgttcatac cgctttgttg ccagttttgt tgccggcttc
    10741 caagatgaac gcaaaccgct ggtgattgcc gttccctcaa ctgccgaagg cttaacccgc
    10801 actaaagagc tggaatttat gtcgctacaa ggcacgaata ctactgcttt agtgtttgac
    10861 aatgtggaat taaaagagga gtggattttg tccgataatg cccccgaatt tttagccaca
    10921 acccgccctg cgttcttagg cttacagttt ggcttaccct ttggcttggt agaaaaaagt
    10981 ttatcggaaa ttgaaaaatc gctctccttc cgtgcaattt taacgtctga atggcaatcc
    11041 caagttgatg cgttaaacga catcaaaaac cgtttattca atggcttaaa tgaagagggc
    11101 tattttgttc gtaacccgaa agagctgttc caagccagaa tcgatattgt taatgtcgtc
    11161 gcccaaacca ttctgttaga actacaagcg agcggtggac gtggttattt gaaaaacgat
    11221 acttcaggct tcagccgccg ttggaaagaa gccgcattct tacctgtggt tacaccaagt
    11281 gcggtgcagc tgagaatggt attggcaaat agttaaacct gagaggcaaa tattgcctct
    11341 ttttatttgc aaaaaattga ataaaaataa ccgcttgtaa atataaaaaa ggacgcatta
    11401 agcgccctaa attttattat tttcgattac gcaaaaggct gcaattcagg gttgattggc
    11461 gtttcggcaa tgttgttcac atagttacat aaggttgcga ggctcacgcc taaaatcaca
    11521 tcaatcgcat tttgttggtt gtaacctgcg gcaaaaaatt cgtttaattg ggcttcggtt
    11581 aatttggctt tttgtaacat tactgcaagg gtgaagcgag ccagagtgtc taatttgtta
    11641 tcgtctatgc gagccgtggc acgtaaagca tttaccactt cttcaggcat ttttaatgct
    11701 tttaaggcga ttttagtatg acctgccacg cagaaaccac agccgttgac cactgctgcg
    11761 gtgatttgta ccacttcacg ttcttcagcg gttaagctgt tacgactatt aataccaccc
    11821 acagtgcggt aagtttccag tgctgttggg gcgttggcta atacgccgat cagatttggg
    11881 ataaaaccgt ttgcttgttg cactgctttt agggcttctt gtgccgtttc aggagcggat
    11941 tcaatagtgt gaatttgaaa ttgagacata aatgttcctt atcaggttgt ttattggtgt
    12001 ggctcatcat aaagaaaagg ctaatttaga acaaatcaaa atcactattt aatataacta
    12061 aaagatctta aagggagtat agttgcctat actccctttg tttatgcaca ggttgcgttt
    12121 ttactcttct gcgtcttctt ttgcccattc taatgagcgg cgaacggctt ttttccaacc
    12181 tttatagcgt ttcgcccgtt tttcttggtc gccgtccggt gtgaaggtac gttcaatttc
    12241 cgctttgtct ttcagttctt ccaaatcttt ccagaagccg acagcaatac ccgcaagata
    12301 agctgcccct aaagcagtga cttctttcac tttcggacgc tcaactttgg tgtctaaaat
    12361 atcggcttgg aactgcatta agaagttgtt tgcggttgca ccaccgtcca cacgtaacgc
    12421 ttgtagtttc gctttggagt cagactgcat tgcttctaac acatctcggg tttggtaagc
    12481 gatagactct agggtcgcac gcacaatgtg gttacggttt gccccacggg ttaaaccgaa
    12541 aattgagcct cgtgcatacg gatcccaata tggagcacct aagccggtaa aggcaggtac
    12601 aacataaaca ccgttagagt catcttcttt agtggcaaaa tattcgctgt caaagctatc
    12661 gtgaacaatt ttcagctcat cacgcagcca ttgaatagac gcaccggcaa taaatactga
    12721 accttccaac gcatattccg gctcgccttt tgcgttacac gcaatggtcg ttaataaacc
    12781 gttttcagat tgaatcgcag tgttaccggt gtgcatcaac ataaaacagc ctgtaccata
    12841 ggtattcttc gcttgtcctt ctttcacgca taaatgaccg tagagagcgg cttgttggtc
    12901 acctgcaata cccgcgaccg gaatacgcac accgccttga ccgccgatat tagtttgccc
    12961 gtaaacttct gatgaatttt tcacttcagg taacattgag cgtggaatat tgagcaattc
    13021 cagcatttta tcgtcccatt gtttggtgtg aatgttaaac aacatagtac gagaagcgtt
    13081 ggtgtaatcg gtaacgtgta cccgcccttg ggttaatttc cacactaacc aagtatcaac
    13141 tgtaccgaac agcaattcgc ctcgttctgc tttttctctt gccccttcta cgttatctaa
    13201 aatccacttc actttagtgc cggagaagta cggatcgact accaagcctg tcgttttacg
    13261 aatatattcc tcgtgtccgt ctgcttttaa tttatcggta atatccgatg tgcgacgaca
    13321 ttgccacaca atcgcattat aaacaggttt accggtctct ttttcccaca caatcgtggt
    13381 ttcacgttgg tttgtgatcc cgattgcggc aatgctatcc ggcttaatac cggcttttgc
    13441 caccacttca tttaaggtgg aactttggct tgaccaaatt tccatcgggt tatgttctac
    13501 ccagcctgct tgcggataaa tctgggtaaa ttcacgctga gccacttcaa ccatattggc
    13561 atttttatct aataaaaccg cacgagagct ggttgtacct tggtcgagag cgatgatata
    13621 ttgtttttcc atagattact cctgagtatt tgctggaaga ttgttatgaa tgaggttttt
    13681 ataaccccaa gcaccgaata atgcaccgca gattggggcg acaatcggaa tgatgaaata
    13741 cggaatttct tttccgcctg ttaaagcaat ttcaccccaa ccggcaagat aagcaaagag
    13801 tttcggacca aaatcacgag ccgggttcat cgcaaagccg gttaacgggc cggttgctcc
    13861 accaattacc gcaatcaata aaccgatgag taatgcggct aaggctttat tcggcaagcc
    13921 gtttttatca tcgccaatcg ctaaaatagt ggacatcaac accacagtaa tcacaaattc
    13981 aacgatgaag gcggtaacca atgtaatatt agggtttgga taggtggaga acacaccggc
    14041 aaagccaaca gtttcgcccc gagtaatgcc ttgagcggct tcggcggcag taaagagatc
    14101 gctatacata aaataaatca gtgcgacaga gcaaaacgct ccggcaactt gggcgatgac
    14161 atagggcaaa attttatgct tttcaaagcc gtagaatgcg gcaagtgcta tagtgaccgc
    14221 agggttaagg tgagcaccgg aaataccggc agaaatgtag attgccatgg aaacaccaag
    14281 cccccaaacg atggaaattt cccataaacc gaaattcgca ccggctacct gagcagcagc
    14341 aacacaacca gcaccaaaga aaagaataaa acctgtaccg ataaattcgc caatacaggc
    14401 attacgtaag gatttgctca taaagtttct ccttaagatg aaaatgattg gaaagtgatg
    14461 aaaaaatgtt tgaatatgaa catatttcgg agtgatattg ccaaagcggc attttttaag
    14521 caaataggaa gtttcaaatt tgtgaaatcc atcacattat ttttctaaat cactcatttt
    14581 taggtatgat gtgagaaaac gttagatgag gaaaaatgat gtcttcaata cttatgattt
    14641 caatcggggc ggtacttgga gctatgtgcc gctggcaatt agccgtttgg ctaaacccga
    14701 tactcagcca attcgccttt ggtactttag cagcaaattg gcttggttgt ttgttaattg
    14761 gtattgcaat ggggtttaat ttaggcgaga gccagcggtt gctgttcatc agcggttttt
    14821 taggcagttt taccaccttt tccagtttct ctttagaact aacggaaaaa ctattagcag
    14881 aaaaatgggg gcagtttgtg acagtgctga gtttacattt aatcggtggg cttttgctta
    14941 ctttgatagg tgtaatgctt gcaagagccg ttacaagcgg tcgtttttgc tgaaaatttt
    15001 gcaaataaaa aacctcccaa acgggaggtt ttgcgagcga ttaaagtagg atattggaaa
    15061 gtaataaacc aaatccgata ctgaaaatca tactaagtaa gcccggtaac ataaagctat
    15121 ggttgaaaat atatttaccg atacgggttg tgccggtggt gtcgaagtca atcgaggcaa
    15181 taatcgggcc gtagttcgga atgaagaagt agccgtttac cgccacaaat accccgatta
    15241 gcaccggtgc aggaatgccg atagcaatcg ctacagggaa taaggttgca accgttgccc
    15301 cttggctgtt taccaatacg gaaagtacga ataatgcgaa agcaaatgcc caaggtgcgg
    15361 tttcaactaa gcctgacacc attgatttca cttcatccat atgaccttgc attaaggtat
    15421 cgcctaacca cgcaataccg aaaatcgcaa tcactgcacg cataccggcg tggaagacag
    15481 aacctttagt gatttcattg ccgtccggtt tacaggtaag aataatcaac gcaccaatac
    15541 ttaacatcac gatttcaatg gtgtgagcca tccccatcgg cttaccatca aacacagggc
    15601 gaagagctgg aattgcgccc attaatacca ctaataatgc accgaatagg aataagccta
    15661 ctgataattt cgcagtcggt tttaacggta attcggttgg attggtagta atgtgatttt
    15721 ctttcacata ttcagggtct tgcaataagc gttgataatt tgggtcgtct tttaattctt
    15781 tgcccatttt atttacaaac acacaagcta aacctaagcc caataaggtc gcaggaatag
    15841 tcacacttaa cacgttaccc aatgtaacac cttgaggttc aaggaaggca accacagcaa
    15901 ctactgccgc agcaatcggg ctggcgacaa tcgcaaattg tgaggcgatt actgccattg
    15961 aaagtggacg ctcagggcgg ataccgttgt ggcggcttac ctctgcaatt acaggtaata
    16021 cagaataagc aacgtgacct gtaccggcaa gcacggtgaa tgtccaagtt acaagcggtg
    16081 cgataaaggt aatgtatttc gggtttttac gcaaaatgcg agtggcaatt ttgatcatat
    16141 agtccaaacc accggctgct tgcatggctg ctgcagccga cacaaccgcc ataatcataa
    16201 acattacatc aatcggtaaa ccggcaggtt ttagcccaaa cccaaatgaa agaatggcta
    16261 agcccaagcc accgaacacc cctaatccga taccgcctac acgggcgcca accaaaatgc
    16321 atagtaacac tatggcaaat tggataataa acatcgcaga cataattttt tgctcctaaa
    16381 taagtaaatg aaactctgtg tttgaaaaaa tctgcatttt tcatcaaatt aatcggcacg
    16441 tactatatca ataaataaaa ctaatctcta taatctaaat caataaaatt tctaattaat
    16501 taaggaataa tatgcaattt tcaaaaatgc acggcttagg caatgatttt atggtggttg
    16561 atggtgtaac ccaaaacgtt taccttaccg aagagatgat ccgtacgctt tctgatcgcc
    16621 atcgtggtat cggtttcgac caacttttat tggttgaacc gccttacgac cctgaattag
    16681 attttcacta tcgcattttt aatgcagacg gcagtgaagt gtcgcaatgt ggcaatgggg
    16741 cgagatgttt tgcccgtttt gttacgctca aaggcttaac caacaaaaaa gacattcacg
    16801 ttagcaccgc aaaaggcaaa atggtgctaa gtttgcaaga agacggcaaa gtgcgtgtga
    16861 atatgggcga accgatttgg gaaccttcgc aagtgccttt tacagcaaat aaatttgaga
    16921 aaaactatat tttacgcacc gagctacaaa ccgtgttatg tggcgtggtt tcaatgggga
    16981 atccgcactg cgtactgcaa gttgatgatg tgaaaaccgc cccggtaaat gaactcgggc
    17041 cgttactaga aagccacgag cgttttcctg aacgggcgaa catcagcttt atgcaagtag
    17101 tgaatcgcaa tcacgttaaa ttacgggtgt atgagcgtgg agcaggcgaa acccaagcct
    17161 gtggtagcgg tgcgtgcggc acagtggcgg tcggcattat gcaaggcttg ttagataaca
    17221 aagtgcaggt ggatttaccc ggtggaacgc tgattattga atggcaagga gtagggcatc
    17281 ctctgtatat gaccggcgat gccacccaca tttatgatgg atttattaaa ctgtaagttt
    17341 tcccataggg aggcaagcgg tttattttta ccgttttttt gcaaaatagg gttggtttat
    17401 acaaaagtac cagcgtggac gctggcacta tcagactgat gtataacttt tgtttaaatt
    17461 caacttatag atggtgcaag cgtcctcgct tgtaccaaaa caggtttcac tcgagtaact
    17521 tcttcaccaa ctccaacacc acaggtaatt cggtaacacc ttctctatcc aaaaaatgcc
    17581 cgccttgatt taaccgaata taatccgcgt gcaaatgttg ggctaacgca tcactgtatt
    17641 ggtgattcac gaccttatca tctaacgcac ttaccacata gcttggaaag ctcgatacgc
    17701 tcggcaaaat cgcataatag ttggtaaagg cagacagttc gggtaagttt tctaccggtt
    17761 gatagaaccc cgacacaaac accgcccctt taatcttggc gtaatttttg gctaaaaaat
    17821 tcaaaatggc aatgcaacct aaactatgcc cgacaaaaac cgtttgatcg gtcagctcca
    17881 cattttgtgc aagatgattt aaccaaccat ctaatgacgg ctttgccgaa tccggcatat
    17941 tcaaccgctc acaactgaca ccacacttcg ctaattcttg ctccagccaa ggaaaccaat
    18001 gcttatcggc attcgccgta taaccgtgta cgatataaac ctgtgccatt tcacacctcc
    18061 tgattcacca aagattgtac catagcttaa tcaggaaaaa acaagcggtg atttttttaa
    18121 atttttttgc aactcaggta aaatagcacg catttacgca aagaggtcat caatgaacgt
    18181 ttggatttta cttgcgattt ccatctgcct cgaaatcgct gccaccaatt tactcaaatt
    18241 aagcaacggc ttcaccaaag ctataccgac tattggctcg ctcgccttat acggtttatc
    18301 cttttacttt ctctctatta ttttccgcac cctaccggtc ggaatcgtgt atgctatttg
    18361 gtccggcgta gggattgtcc ttaccgccct tgttgcttat tttgctttcg gacagaaaat
    18421 tgacttggca ggcttaatcg gcatcagcct cattattgcc ggtgtattgg tgattaattt
    18481 gttttctaaa gtgtgataca agcggttgat tttacaaaaa aatctgcaaa atcaaccgct
    18541 tgtaatagtt ggcaagaatt ataacccctt taaccgccgg taaagctcag taaatttttg
    18601 gcgtttttgt tgatattctg catagcgggc aggggttggt tggtaaacct gttccagggg
    18661 taacggctga cagaacgtag caatatcctg atttgggttt agcgcaattt gagcgagttt
    18721 tgccgcccct aatgccggac cgacatcacc actggtgcgg tattcaaagg ttttaccgct
    18781 aatatcggca agtagttgtc gccagtacgc actttttgct ccaccgccaa ttaatgcaat
    18841 attgtcagca actacaccag tttcgtggag tacctcaatc ccttcagcca aagcgaaact
    18901 taccccttca atcacggctt ttgccatcgt tatttggttg tcattatggc ttaatcccca
    18961 aaatacgcct ttcgcataag ggtcattatg cggtgtacgt tcacccgata aataaggtaa
    19021 gaaaatagca tcagatgccg tttggcttgc ttcaacctgt tggaataaag tcgatacatc
    19081 ttttagtccc aatgatttta ctgcccaatc taccgcagaa gcggcactta atattactga
    19141 catcagatgc caacgatcag gtaaagcgtg gcaaaaacta tgcactgctt tttgcggatt
    19201 agcctgaaat ttatcgctca ctacaaaata aacccctgaa gtccccaatg agagcatagc
    19261 ttgcccggtt tggtataagc caatgccgat tgcaccggca gcattatcgc caccgcctgc
    19321 cacaatcggc acagatttca ttttccattg ctcggcaagg gttggacgta aataacctgt
    19381 gatttgattg ccttcaaaca gtttcggcat attttcaatg tctaatccgc aagcatttag
    19441 tagcgatttg ttccaattcc gtttacccac atcaagccac attgtgccgg aggcatcaga
    19501 catatcagag gcatattcgc cgctcatcaa caagcggaga taatctttcg gtagcaagac
    19561 tttgctgact ttttcggcaa tatcgggctg gtgtttatca acccaacgca atttcggagc
    19621 ggtaaagccc ggcatcatca ggttgcctgt aatttcacgg ctatttggca caagctgttc
    19681 taactcttcg cattccataa aactacgtcc gtcattccat aaaatcgcag gggaaagtac
    19741 attatctgcg ttatctagta aagtcgctcc gtgcatttgc ccggttaaac taatcgcttt
    19801 tactgcttgt aaatcttgag tagcggaaag ttctaacacc gcttcattgg tggcattcca
    19861 ccaatcttgc ggattttgtt cagaccacaa tgggtgtgga cgaaaaatag gaagtgattt
    19921 ttgtgtgatg gcaataatgt gttgattttc atcgagtaac acgactttca cacccgatgt
    19981 gccgagatcg attccaatgt acataaatat tcctttgtta ttgaatacaa gcggtcattt
    20041 tattcggaac atttgcaatt ttaagttttg gagatctatc acccctcttt agcaaagagg
    20101 gggatcagat cgagcttaac tttgcaaaaa attgataaaa aacaaccgct tgtagattaa
    20161 cggtaaatat agctatttac caaattttcc aaatattctt gctgaccgga aacaggtttt
    20221 ggatctaacc cttgggtttc cactattttt gccaaatctt caaggcttgc tttaccgttt
    20281 agaatttgta agcccaaatc ttggttccag ccagcgtagc ggttatctac tactttctgt
    20341 aatgcttgat cttccagcat ttttgccgca cgttttagcg aaagggctaa cacatcaatt
    20401 gccccgatat gtgcgtggaa taaatcatac ggatcggtac tttggcggcg gattttcgca
    20461 tcaaagttga aaccgcccgt tgtgaaacca cctgctttta agatttcata catcacaagg
    20521 gtgttttctt ccacgctgtt tgggaattgg tcggtatccc aacctaattg cggatcacct
    20581 cggttggcat caatcgagcc aaaaatatct aatgcggtgg cagtcgcaat ttcgtgttgg
    20641 aaagtatgcc ctgcaagagt ggcgtggttt gcttcaatgt tgactttgat ctctttttct
    20701 agaccaaact gttttaagaa accgtaaacg gtggcaacat cgtagtcata ttgatgtttg
    20761 gttggttctt gtggttttgg ctcaattaat aaagtgccgt taaaaccgat tttgtgttta
    20821 tgttccacca ctaactgcat aaagcgaccg atttgttcac gctcacgttt taagtcagtg
    20881 ttgagtaggg tttcataacc ttcacgtccg ccccataata cataattttc gccgcctaag
    20941 cgtttggttg cgttcattgc ggtaaagact tgtgctgcca cccaagcaaa gacttccgga
    21001 ttcggattgg ttgaagcacc cgacatatag cgaggattgg taaagcagtt cgccgtaccc
    21061 cataataatt tcacgcctgt ttcagcttgt ttttgctcta aaatatccac aatagtgttg
    21121 aaattgtgct gatattcttt gaaattgttg ccttccggtg caacatccac atcgtggaag
    21181 cagtagtaag gaatgcctaa tttttggaaa aattcaaagg caatttccgc tttttgcttc
    21241 gcaccttgca gtaaatcccc cgttttttgc cagcttctgt caagcgaacc cacgccaaac
    21301 atatcgttac ccgtccagca gaaggtgtgc caatagcaaa ccgctaaccg cagatgttct
    21361 gccatcgttt tgcctaaaac cacttcatcc gggttgtaat gtttgaatgc aaacgggttg
    21421 gtggaattag ccccttcata ttgtactttt gagattttgt cgaaatagtt agacataata
    21481 atgctcctat gaattggaaa aataatcttt cggtaacatc atcggaaaat tatgcaaaac
    21541 cttcaattac gatatttcgt ttttggattt cgattattgg ttaatgtggc gtagatcaca
    21601 aaagtgaaaa atcataatga tattaataaa tatgataatt gaacttcatt tgaaataagc
    21661 ccagactgct tacgggcgaa aaagatgctc acattttcat aaccaatgag gtgaactatg
    21721 aaacttaagt ctaaattatt agccgttgct acggcgactt taatggtact cagcaatgct
    21781 tctgttgcta aagatctaaa aatcggtatg tcaattgatg atttacgctt agagcgttgg
    21841 caaaaagacc gagatatttt tgtgaataaa gcggaaagct taggagcaaa agtcttcgtt
    21901 caatcggcaa atggcgatgc aaccgcacaa atctcacaaa ttgagaatat gttgaataaa
    21961 gatattgatg tattggtgat tattccgttt aacggcgaag tgttatccaa tgtgattgcc
    22021 gaggcgaaga aagaaggtgt taaggtactt gcttacgacc gcttaatcaa taatgccgat
    22081 gtggattttt atgtttcatt tgataatgaa aaagtaggtg aattgcaggc taaaagcatt
    22141 attgggcaaa agccggaggg gaattatttc ctaatgggtg gttcacctgt agataataac
    22201 gcgaagctgt tccgtaaagg tcaaatgaag gtgctacaac cgttaattga cagtggaaaa
    22261 attaaagtgg taggcgatca atgggtagat tcttggcttg ctgaaaaagc attacagatt
    22321 atggaaaatg cactaactgc taataaaaat aatattgatg cagttgtagc ttcaaacgat
    22381 gcaaccgcag gcggtgcaat tcaagcgtta agtgcccaag ggttatccgg caaggttgca
    22441 atttccggac aagatgcaga tttagccgca attaaacgta ttattgccgg tacgcaaacg
    22501 atgacagtgt ataaaccaat taccaattta gccgacaaag ctgccgaaat tgctgttgca
    22561 ttaggtaaag atgagaaagt agaaatgaat gctcaattaa ataacggtat gaaagaagta
    22621 ccggcatatt tattagagcc ggttgttgta actaaagaaa atattgatga cacagtaatt
    22681 aaagacggct tccataccaa agatgcagta tataaataaa tcactttaat ttttctagat
    22741 ggcgaggctt cctcgccatt tttgaaaata aggggctaac taagtatggc gaaattatta
    22801 gaaatgaaga acatcactaa aaaattcggt gaggttgtgg cattaaataa tatttcaatt
    22861 tcccttgaaa ccggcgaaat actttcttta tgtggtgaaa atggttcagg caaatcaact
    22921 ctgatgaaag tattatgtgg aatttatcct gccggtgatt atgaagggga aatctatttt
    22981 tccggcgaaa aattgattgc cagaaatatc aaagatactg aagaaaaagg catttcgatt
    23041 attcaccaag aattgacctt agtaaaaaat atgtcgattt tggagaatat gtttttaggg
    23101 aatgaaatta ccaaatcagg gattacagat gacaatgaaa tgtatctgcg ttgtaaaacc
    23161 ttacttgaac aagtgcagtt ggatattgac ccaaacacca aagttggaga gctaggttta
    23221 gggcaacaac agttggttga aattgccaaa gcattgaata aacaggtacg gttgttgatt
    23281 ttagacgagc ctaccgcctc gttaaccgaa aaagaaaccg agattctgct gagtttaatt
    23341 aaagatttac aagctcacaa tattgcttgt gtttatattt cacacaaatt gaatgaagtg
    23401 aaagccattt ccgacaaaat ctgtgtcatt cgagacggcg aacatatcgg tactcgcagt
    23461 gcagagggaa tgagtgaaga cgatattatt acgatgatgg ttggacgtga gattacctcg
    23521 ctctacccac acgagccaca caatattggt gaagaagtcc ttcgagttga aaatttaacc
    23581 gcttggcacc caaccaatac acatatcaaa cgggttgata atgctaattt tgtactacgc
    23641 caaggtgaaa ttttaggtat tgctggtttg gtcggttcag ggcgaaccga gatggcacaa
    23701 tgtatttttg gctcttacca aggcaaatat caaggtgata tttttctgca taagcaaccg
    23761 attaaaatca aaaattgtgc acaagcgatt gaaaaccata ttgtgatggt gccggaagat
    23821 cgcaaaaaac acggcatcat tccaattatg ggggtgggta aaaacataac tctctcttca
    23881 ttaccgcaat tttgttttgg caaaaaagta ataaatgaac cgctggaaga aaccattatc
    23941 aaccaatcta ttgcaaaact gaaagtgaaa acttcctcgc ctgaattagc aattggacgg
    24001 ttaagtggcg gtaatcagca aaaagcaatt ttggcaaaat gcctattgct taaccctaaa
    24061 atcctgattt tagatgagcc gacccgtggt atcgacgtgg gagcaaaata tgaaatttat
    24121 aagctcatca accaattagc taaagaaggt atggcaatta tcgtcatttc ctccgaatta
    24181 cctgaagttt taggcattag tgatcgtgta ttggttatgc accaaggcaa aattaaagca
    24241 gatttgatta accaagacct tacccaagaa aaagtaatgg aagcggcact caaggagtaa
    24301 cctatgaata agttaaaatc gattaattta caagtttata ttatgctgat tgcaattgtt
    24361 gtgattatga gctttttctc tattgcaact gacggtgctt atttaagtgc cagaaatatc
    24421 tccaacctgt tacgccaaac ctccattacc ggtattcttg ccatcggtat ggtatttgtg
    24481 attatttccg cagaaattga tttatcggtt ggctcgctaa tgggcttact cggtggattt
    24541 gccgccatca gtaatgtatg gtggggctgg ccgctctcgt taactattat agttacttta
    24601 attttaggcg tcatgctggg tgcttggagc ggttggtggg ttgcttatca aaaagtgccg
    24661 tcctttattg taactcttgc cggtatgctg gcgtttcgtg gtgtattggt cggcttaacc
    24721 aacgggacaa ccgtttcacc gattagcaat gaaatgacaa ccattggtca aggctatttg
    24781 cctgatatgg tgggtatgat tttaggtact atcgggatat tttgctttat tgtttggggt
    24841 agctaccaac gcaaagccag acaaaaactc aatcttactg tgccttctat gtcgaaagac
    24901 acgctgaaat acggactagt tgcaattatc gtattaggtg cgatctacct actcaatgat
    24961 taccgtggtg tgccattccc ggtattggtg ttggtgattc tagccattgc cggcacattc
    25021 ttggccagaa aaaccgcctt cgggcgtcac atttatgcga ttggtggcaa cattgatgcc
    25081 gctcgcctct cggggatttc agttgaaaaa gtcaaacttg cgatttttgc cttaaacggc
    25141 ttattagttg ccattgcagg cttgatttta agctcacgtc ttggagccgg cgcaccttcc
    25201 gccgggcaaa atgccgaatt agatgcgatt gccgcctgcg tgattggtgg agccagttta
    25261 gccggcggta tcggtagcat ttatggcgta gttattggag catttattat tgctttgctg
    25321 gataacggta tgagtatgct tgatgtgcca accttctggc aatatatcgt taaaggtgga
    25381 attctactgc ttgcagtttg ggtagatacc atcagcaaga aaaaagccta attggataac
    25441 aagcggtcaa tctcgactat ccaacgggaa atccggcata atcgggctaa aaatgggaac
    25501 tatgttgcca gacatactca acaattttac cgtcaacgtc agattaccaa aaatgaccgc
    25561 ttgtaattta gaggagtgtt tagagcggaa ataacatcgg aatcacaaac accgacacca
    25621 gcatcaccag tacagtaaac ggcacaccaa ctttaacgaa atcaataaat ttatagcctg
    25681 ccggtgctaa taccatcgta tttaccggtg aagaaaccgg cgtcataaaa gcggcggagg
    25741 ctgaaacagc gaccgtcatc acaaaaggtg ccggtgagta gccaagttgg ttagcaatcg
    25801 caatggctat cggcataacc aaaattgcag tcgctgtgtt ggaaataaaa gcactgataa
    25861 tcgctgttac tacaaataat actattagaa caaaatgtac atctaagcct tgcattgcat
    25921 caagtagaag cctaacggtt aaatccacac caccggtttt ttgtaaggca atagaaaacg
    25981 gcatcatccc cacaatcaac acaatgcttg gcatatgaat cgaatcataa gcacttttca
    26041 tatccacaca gcggaacttg cctaacatta agcaggcaat caacgctgcc atcacgtttg
    26101 gcacaatgcc ggtaatcatc agcaacacca tagtcagcac tgagaacaga gcgtgagggg
    26161 cttggcttaa cgccggaggg gcgtttttac tttctgccgg atctctaagc aagaagaaat
    26221 ctcgatgatc gtcgtccata cggtgaattt gttgccatac gcccatcacc aataaaatat
    26281 cgccgaattt gagtggcgta tcgagcagtt cctcttgtat gatttcatca ccacgtttta
    26341 agccgacaat gcttaaatgg tatcttgagc ggaatttgag ttcccgaata gttttaccga
    26401 tacattctgc ttcaggcata accgaaattt ccgccatacc caccgagcga tattgtgagg
    26461 aaaagtattc gccttttagc tcaatcgggc gaagtttgag ctgttcacag cgttgttggt
    26521 aagtttcatc atcgagggca gtatcaagca gtaaaatatc tttcggctgg aaaatttcgt
    26581 ttaccgaggc actgacggta atttttctaa accgcttagt gcgttgaatt gcaatgatgt
    26641 ttagcccttg ctcaatccgt aaattcagtt gcccgataga tttgccgaca cagtctgaat
    26701 ctgcttccac cagcaccatt ttggctcgct ctttaagttg atactcttca attaactgcg
    26761 acattgaaaa tttttgagcc tgactgtgag tattgctttg ctcaccatta tccaaccaac
    26821 ggcgggcaat cagcatatag ccgatgccca tcactaaaat aattaatcca atcggagtaa
    26881 aactgaagaa attgaagcct tcaaaacctg attttactaa ttcggaatga gcaacaaggt
    26941 tgggtggtgt ggcaattaag gtcatcatac cgctgataag tccggcaaca cttaacggca
    27001 tcattaaccg gcgaggagaa attcccattc ccgagcaaat ggcaagcacc accggaatga
    27061 aaatcgccac aatgccggtg gaactcataa aagatccaag cccggcaata cagagcatta
    27121 gcagcaccag cactttggtt tcgctcgctc ccgacacccg catcaaccaa tcgctgactt
    27181 ggttcgccac gccggttcgc accaaacctt ctccgacaat aaagagcaac gcaatcagca
    27241 ccacgtttgg gtcacttaag cctgccaaca cttctttgac tgataaaata ttagtcaggc
    27301 taaaaaacag cattacaatt aaggcaacca cgtccatacg caacttgttt tggataaatt
    27361 gccaaatcgc aaataccaat aacgccatta cccaaaacag ttcttgctta agaaaatccg
    27421 gcacaaaaga gagcaacgat tgcataacca tatcctccaa aatagttagg gatagattac
    27481 accgctttaa gcaaagaaaa aagccgataa gatcaaaaag aaaggaaaaa gcaatcacgt
    27541 acggcgtgaa tgctcagtac aagcggttag ttttgacgaa atttttacaa tgggctttcc
    27601 acattcagtt gttcgtcaat ttcagttacc gccaaaatca ggcttgaacg taccattttt
    27661 tccaaccgca tttgctgcat ttgaaagagc atcgaatctt tattcgctgc actcgctttc
    27721 atattcagca attcattgat aggcaaaaaa tcaatatggt gcaaatcttt ggcaaatttc
    27781 actaccgccg ggtgagaaaa tgcgaactcg tcttcttcct cattcaaatg ctgtttgact
    27841 tcaaggaaaa gcgagatatc ttcaaacact ttaggtgaga tcacccccaa cccaagcaac
    27901 acttttaaac gtacttcaat gtcagccagc ggtccttgat gttcaaacaa ggaatccacc
    27961 acggatttta acgcaaagtc cgttttgcga aacactcgtt caatcaactg ttcaaccgct
    28021 ttggcaaagc gttgattggc aatgctaagg aaaccacgca gggtggtttc ctcactgagt
    28081 ttgtcgatgt aatccatcat agtgtcaaga attattgata taattccacc acttgagcaa
    28141 tgaccgcttg gtcggttaaa ccggtgtatt tttccaccgc tgccgccaca ccgtgatttg
    28201 caataaattc ggcaagttcg accgcttgtg ggtcttcctc attgcgatat tgcaatgcca
    28261 tcaccacgcc tttgaccaag ttttgatacg gtaagccata ttccaacgtg ccacgcaacg
    28321 gtttgatcaa gcggtcgttt tcgctcagtt tgcggattgg ctcacgccct acacggttta
    28381 cgtcatcgtt taagtacggg ttggcaaaac gtttgaggat tttttcgata tacgctgaat
    28441 gggcttgtgg gtcgaagccg taacgtttaa tcagcacagc accgctctct tccattgtgg
    28501 ctttcacctg agttttgatt tcctcaatcg caatcgcctc tttaatccat ttcacgcccg
    28561 cttgtttgcc caagtaggca cagattaaat gcccggtgtt cagggtgaac agtttgcgtt
    28621 ccacaaacgc catcaggtta tcggttaatt ccattccttt gatgtttgga atgtcacctt
    28681 tgaactgggt ttgatccaca atccactcgc tgaactcttc caccgtgact tccaacggat
    28741 cagcagggtt cggctcggca ggtggtacaa ttctgtccac cgcactatca acaaaaccaa
    28801 ccactttttc aatttctgct tgctgctcgg cagttaaatg ctcaaaaatt ttacctttaa
    28861 agaaagttgt gccacgcacc atattttcac aggcgataat gttcagcggt tgggtgttgc
    28921 cactttccac acgagccact aacgctttgg cgaacagtgg ggcaataaag ccaagcacat
    28981 tcgggccgac tgcggtggta attaaatcgg tgtttttcac ttgctcaatc acggcatttt
    29041 cgtctttcga gttaattgcc gcaatgtttt tgacaatttc cacacggctg tcatcgccca
    29101 cgattttcac gccgtattgc ttgttttggt tgatttggtc gatttgagtt tggttaatat
    29161 cggcaaaagt cacgaaaatg cgagcatcag caagcaattt gccgataaag ccacgcccga
    29221 tattaccggc accaaaatgg agtgcgttca ttgatgattg ttcctctata aaacgtaggg
    29281 gcgggtcctg tgcccgccct aaattttgta ggataatggg cgggcacagg acccgcccct
    29341 acacaacagg gtgattattt agataataat gctaacactt tttccacgtc atttgttgag
    29401 gtcagtgttt tcattgcttg ttcatcgtcc agtgcgttgg taattgcact taaaatttgg
    29461 atatggtcgt tgccttttgc tgcaataccg accaccagtt tcgccacgcc gtcttcttca
    29521 tcggtgaagc gaacgccgtt cgggtattgg cagaccacga taccggtttt cagtacgctg
    29581 tctttggcat cagatgtacc gtgtggcaca gctaagcctt cgcctaagta agttgaaacc
    29641 atttgctcac gggcgaacat tgcatctaca tagcttggtt gaacaaagcc cgctttaact
    29701 aactgctcac cggcaaagcg gatcgcttct tctttactgt tagcttgtaa gcctaaaaag
    29761 acttgggcta cttgcaattt taacccttcg gcttgaggtt cttccaccgg gtttgcaaaa
    29821 ttttgtgtat tttgcaccgc ttgttgtggc tggtggttag ccagttttgc cactaagctg
    29881 tcatagaacg cactgtctaa gaagttagtg agcgataagt gttgagccgt tgccacacgt
    29941 tgtgttgcac gagcggttaa gtctttgtgg gtaatcacca gttgtgcgtc ttgcggtaag
    30001 tcgttgatcg ctaagttttg tacttcaatg tttaaacctg ccgcttgcac ttttttgcgt
    30061 aacatactcg cacccatcgc acttgaaccc ataccggcat cacacgctac atagattttg
    30121 ttgatgttac catttactac gcctttgctt tctgctttca tcgcttgagt ttgtgcttgt
    30181 gcatcttcca atgaggcttc acctttttgg gttttgagta agattgacgc aatcgcaaaa
    30241 gtcacggcac aagaaagaat cacggaggca acaactgcaa agtgagcacc cggtgcggtc
    30301 attgctaaaa tcgcaaaaat agaacccggg gaagccggtg cgattaaacc gccacctaat
    30361 aatgagttgg ttaaaacgcc ggttgcaccg cctgcaatca ctgcaagaat taagcgtggg
    30421 ctcattaaca catacgggaa ataaatttcg tgaataccgc ctaagaagtg aatgattgac
    30481 gcactaccag cggtttgttt tgctgtgcca cgaccaaaga acatataggc aagcaagata
    30541 ccaaagcccg gacccgggtt cgcttcaatc aagaacagaa tagattttcc cacttcttgt
    30601 gcttgtgctg tacccaatgg cgtgaacaca ccgtgattga tcgcattgtt taagaatagg
    30661 attttcgccg gttcaacgat gatagaggtt actggcagaa gaccagcttc aaccaacgcc
    30721 cccacacctg cacctaatgc cgcagagatt tgggtgacga tacctccaat gaaagcaaat
    30781 gaaagcattg ccaaaatcat accgatgatc ccgaccgaga agttgtttac caacatctca
    30841 aaaccgcttt ttactttgcc gtcaatcgct ttatcgaagg ttttgatcgc ccagccgcct
    30901 aatggaccta caatcatcgc acctaagaac atcgggattt cagaacccac gataataccc
    30961 attgtagtaa tcgcacctac aaccgcacca cgctcaccac cgactaattt accaccgctg
    31021 taaccgatta acagtggcag caagtaagta atcatcggac cgaccagttt agcaaaagtc
    31081 tcgttcggca accaaccggt tggaataaac agggcggtga taaagcccca cgcaataaag
    31141 gcaccgatat tcggcatcac catattcgat aagaaacgcc caaaaccttg gactttcact
    31201 tttaaattag acatagaaac ctcaaaaaag gagagaaaat tagggttgaa aaaatcgtgc
    31261 ttaaattagc accactttca aaagttacaa aaatgtttct ctcataaatg tgaagtagat
    31321 cacattaaat ttttttgggc atatttgaaa atgtgatcta tctcacattt ttggagtaaa
    31381 gatggcgtaa gcgtcctcgc ttgtgccaag cataattcta tttttgcgat ctacatcgaa
    31441 aaaataaaat gaattgctaa ccctaactgt tcaccgatat tactatcagc ataaactgca
    31501 ctttagaggt tcataataag tagaaaatat aagttttatg agaagagcgg agcctatttt
    31561 gttagttttg caacagttta ttggattgat ctatttattc gagaagatta ttttcaaacc
    31621 attatccaat cattgaatta ttgtcgaaaa caaaaaggga tgatttttta tatggctact
    31681 gcattatgcc aagccatatt catttattat ttcaagcaaa agaccaaaac ccgtcagatt
    31741 taataagaga ttttaaaaca tttacatcta aaatgttgat tcagcaaatt aaaacaaatc
    31801 cccaagaaag tagaaaagaa tggttattat ggatgtttac tcgggcagct aaacaacgaa
    31861 ataatgtcaa gcaataccaa ctttggcaac atcataacca accgataaca atctattctc
    31921 agaaattttt tgatgaaaag ctaaactata ttcacaacaa ccctatcgta agcggttttg
    31981 tatgcgaggc atttgaatgg aaatatagca gtgcaagaaa ttatgctaac aacttacctg
    32041 ttttattaga tattgatata tgtcagtgaa cggcacaagc gtaaacgctt gcgccatcgt
    32101 tacatctata aattaactag agaactccat caatcccgtt actttaccct catcattcac
    32161 tgcaattaat gagtgaattt tcagctcttt cattaaatct tcggctttgg ctaaaaattc
    32221 ggtatcgtta atggtttttg gattacgggt cataatttcg tcagcggttt tatttaggct
    32281 ctccgccccg aatttcgcta atgtgcggcg gatatcacca tcggtaataa tgccttccaa
    32341 tttatcgtct tgcataatta ccgccacccc catacgccct tcgttcatca cagataaaca
    32401 ttcggtaaag gtggtgtcta agctggcggt tggcacttta cgcaccatca catctcgcac
    32461 tctgttcaat aatttacgac caagactgcc acccgggtgg tagcgggcaa aatcttcagg
    32521 gcggaaatca cgggctttca ttagcgcaat ggctaacgca tcgcctaaag ccatcgttac
    32581 caaagtagag ctggttggag ctagattatt caggcaggct tcacgctcta caccaatatt
    32641 taaaatcaca tcggcatttc gcccaagtgt ggagtaagga ttacctgtca tcgcaataat
    32701 tttattgccg aaatttttca ggcttggaat cagtttattc acatcatcag tttcaccact
    32761 gttagaaatc agaattacca tatcaatcgg tttcagcatt cccaaatcgc cgtgaaaagc
    32821 ctctgtcggg tgcaagaaaa agctcggtgt accggtggag gcaaaagttg ccaccatttt
    32881 tttgccaacc agccccgatt ttccaatacc gccaacaacc actcgccctt gacaagcgag
    32941 tatcatattt accacctcat taaattcttc agaaagtcga tggtttagcg taataatctg
    33001 ctgagcatag aggtttaacg tttcttttgc actgtctaaa tgattcattt ttacgccctt
    33061 atacaactaa cagctcaatc cctttcgctt taatctgttc tctcatttct tgagataatc
    33121 cttcatcggt aatgatagta tgaatttttt ctaacccaca cacaatattc ggacttttac
    33181 gtccaaactt actggagtcg gcaagtaaga cgattttatt tgccgcatca cacattagcg
    33241 aactgacttt atatacttca ttgaaagtcg ttaatccaac gtctaaatct agtccgtctg
    33301 tgccaataaa gagtatatca aaatttgaac caataaaagc ggattcagca aaataaccgt
    33361 gaaatgaagc tgatttctca cgaaatgtac caccggaaat cagtaagtta taggatttat
    33421 tgagtttcaa aatctcattg aaaatagtta aactatttgt cattaaagag aggttatcaa
    33481 atgcttttaa atgggggatc atttgtaaca ctgtgctgcc tgcatcaaaa ataatagagt
    33541 ttccctcatt aattaactct gcagcttttt gcccgatagc ttgcttttgt tgaagattaa
    33601 tgccggtttt ctgtgcaagc ggtttatcgt ctttgtcctc tttattgatt ttctcttccc
    33661 gttcaagaat ttcttcatca attaagacta cactgccata agtacgcaag actttcttat
    33721 cttcttcaag tgaagttaaa tctttacgaa tggttgcacc agttagctta aaatagtctg
    33781 ccagcacgtc cacttcggtt ctaccgtgct gagctaaata ggctaaaatt tgctgtcttc
    33841 tttcaaaagg tttcattgtt cctccaattt agcaaagatt ggagcgagct aatctgtgct
    33901 aatgctgttt taatttttca atgacatcta gtagcgatgc tattctttta tcggcaattg
    33961 aaaatttttc attttgataa tcggtattca ccaaaaatgt agttaaatta gcggctttgg
    34021 cggcaattaa gccggttaca ctgtcctcaa taacaataca ctcttgtttt tcaatgccta
    34081 attttttcac tgcactcaaa tagaccgcag ggtgtggctt gccatattct tcgtccattg
    34141 cactgcatcg aatcgggaaa taatcccaca gttttaagcg atcaaacacc gctttaatca
    34201 ccaccggcgg cgatgaggtt gcaacggcta acttgatctg attagatttt aagaaatcca
    34261 ataattcata taaacccgtt aatgctttgc cgtgttgttc aatcgcagca caaccgtgtg
    34321 ccaaaatggc ttcacttaat gcttggttgg aaacagataa ctgaaatttt ttaatccaag
    34381 tgtcgctcaa ctcatcaatc cgcttacctc tggtgagttg ttcgcaatct tgctcggtga
    34441 tattaatacc taagtcggct aatactgcaa tttgagcctc tgcccattgg ggttctgaat
    34501 caatcattac cccgtccata tcaaagatga cactattgat ctgcatattt attctccatt
    34561 atctcatttt taactaaaaa aatattcaaa atgaaataaa aaatcgcatt atttttgatc
    34621 tgagcaacta tatccggaag atatcgccac ttttaaggca aattgtgaac gtttatcgct
    34681 tggaaagatt ttttccggca caatttgatc aagagacaaa ccaatcacag acgctaattt
    34741 ctgcggtctg gcaaagacgg aaaatccttt catacaaagg ctatctacca cttttttctc
    34801 atcatcaaac gccaacacaa taaccgattt aggattaaaa cgaccactat tacgcccaac
    34861 cgttactttg cctttcttac tgatttccat aaaggcttgg ttaaaggctt taatttgaag
    34921 ccaacccaaa acggcttgta acagccacat tatcattgcc aatataatca gaagtgtaat
    34981 tgaatccatc attgctcctt aataaaaagc cacagagcct aaacgtacct gcgatttcaa
    35041 aacaatacgc tttcccctgt ggcatattaa ccggtgtggc gaattaacaa gcggtttatt
    35101 ttagtcattt tttgcaaatt gttgtaaaaa aatcgacaaa tttgaccgct tgtttgctta
    35161 gaacattacc tgaccaccgg taatattaat agactgtcct gtgcaataag ctgcctcttc
    35221 gctggcatag aattttaaga cattcaacac atcttgataa tcacagccac gttttaaagg
    35281 cactttatca atataaactt gctctacttc gctttccgga atgccgagtt ttgccgcata
    35341 ttgcggaatt aacgattgga acatcggcga tttcaataaa ttgcctaaca ttaatgaatg
    35401 cacggtaatg cctttatctg ccaagtctaa tgctaacgat tgtgttaaac caacaccacc
    35461 aaatttcgct gcactataac cggaattata cttactccct actttacccg atttagaatt
    35521 aatttgaatg atacgccctt taataccgtc acgaatcatt aagcgggaga agtgttttgc
    35581 ggttaagaaa taaccggtta aattcacttt taccgacaaa tcaaaatcgt ttaagttgaa
    35641 atcccaaata ggcgatgctt tcgctgtgcc tgcactgtaa accaatacat cggcacgccc
    35701 gaatacggta tctgttgctt tagctagtgc ttcaacgctt ggttcatcag ccgcatcgac
    35761 ttgcacccca atcgcatttc cttcgccttg tttggcatta atgtcggctg ccactttttg
    35821 tgcattttcg ccgtttgagt cagcaaccac cacacgataa ccggcatctg ccaagccttc
    35881 agataaaaaa gctccaagtg tttgaccgcc gccaacaacg atagcgactt ttttttcatt
    35941 gttcataatg tattcctcta aattttcaat ttgaaattat ttttaatccg tcattaatca
    36001 cgcttgataa tcaaggttga gttctctgtt aagccttgcg gcggtgttcc gataacgtga
    36061 acgcttcccg gaaattccgc atcatttgcc ccgtcaaaac ggaaggtaat gtgtccgagg
    36121 gcggataagt tttcagatgc aacgctacca actgccgtaa tcggataagc tacgccatca
    36181 aattcagcga tatcgcctac ttctaaaggg ctacttaatt cagatggatt atgaatgaag
    36241 cagtaatctt ctaaatctgc cggtgcgcct tgtttaaaag tgattaacat atcgtctgtt
    36301 aatgattcct gagcaaaatt cccgattttg gtaaaggtcg tttgataaat aatcgtcata
    36361 taaatgttct ctcgttttga caagcggtgc attttgcaaa aaatttttca aattccaccg
    36421 cttgtatcgt ggttaattat tggtagatga atgcggaaac tgcccacgca atcagcacgg
    36481 ttggtgcacc ggttaagaaa cggctgacta atactgacgg tacacccacc cgtacggtat
    36541 cttgttttgc ttccgccatt gaaagaccca caggaataaa atcacaagcg gcttgagcat
    36601 taatagcaaa gagtgccggc agtgctaaat gtggcggaat attgcctaaa ccgatttgcg
    36661 taccgactaa cacgccgata acttgtgcaa tcacggttcc cggccctaag aatggggaaa
    36721 ggagagggaa ggaacaaatc aacgccagtg taactaaacc gattgggctg tttgccagag
    36781 gggtcaagcc gtgggcaatc acatcaccta aacctgacgc aatgatgatc ccgattaacg
    36841 cagaaacaaa tgccataaac ggtaaaatgg ttttaagcac cgtatcaata gtgtctcgtc
    36901 cggcttggaa gaataacgcc gccaccgatc ccattcccat accgattttt gctaataaac
    36961 catcactttg ttcggtgatt tttttgctgg agtcatactc ttttgccgtt gctgtcgttg
    37021 atgaagttgg ttttgcttgc tctgttgcca caggcacact tccatcggca tcaattaagc
    37081 gaatattgtt ttctttcacc gctgaaacat aaatatcttc cacaataaat tctgctaaag
    37141 ggccggattt accggtggag tgaatgttaa tggttggaat acggcgtttt gggtaaagcc
    37201 cacagcgtaa taccccaccg caatcaataa tcgcaatccc gatttcttct gccagcggtt
    37261 cgccttcttt aaaaccatca accgcttccc agccggttaa ttccaccaat ttattaacaa
    37321 tcgcagggcg agtgccgccg gtcatataaa gcactttttt gccttccaca acaggtaatg
    37381 ttaatggacc accccaacca ccgttgcctt tttcaatgta aatcgctttg ttgctcataa
    37441 tctattcctc tgtatttcag ttacttggtt agtgggataa atgtacttct ctatctaagg
    37501 tgatccccat tcgtttttca aagaatgcgg tggtgaagtc tgttatccag ccacggaaga
    37561 aattggtaaa cataccgact aataaataac tgatggctaa aggcccaagt ggtaagccta
    37621 aggtggttaa accgctggca atacctaaat aaacgaataa ttcacccggg ttgatgtgag
    37681 ggaataagcc gttcatagag tggcaactat aagaggctgc ggcgtagtag cttggtttgt
    37741 aacgctccgg taagaaacgt cctaagctta atgtcatcgg gttacagaaa acgaaagtgc
    37801 cgatcaccgg taagatcaaa taacgtgaaa tagggtttcc ggcactacgt tgagcaagtc
    37861 gttcaatccg atcttgccct acaaatttaa tcagggcatt cattacaacc aataatgaaa
    37921 ttaagagtgg caaaataccg tttatcatac cgactaaggt ttcaccgcct ttttggaaca
    37981 ggccgataaa ccattctgct ccgtgtgtaa ttgcttcaat cataccgatc tccgtttttc
    38041 aaaataaaat atttttcttt catctctgtt atgagatgtg caaatagtaa attattttta
    38101 ttttaaaaac tgtgacagcg atcacaaaat gagaaacaac tttcaaaata aattattttt
    38161 tcattttaaa tcaaaacaaa agtgaagttt gaatattaga tggggaaaaa aggaaggcta
    38221 aattaaaaaa gctcaaacaa taatgtttga gctttggttt tattcaatga cttttgcagt
    38281 atatttgagg tttccgaccg cttgtatcag ggcttggtca tccacacttt cttttaccac
    38341 cacagtcgct tttcgctctt tcattgaggc tttggtggaa atcacacctt ctactgctcg
    38401 tagttcttta ttgactaaat aaacgcagag ctggcagtac atttccggaa tatggaggac
    38461 gacggttttc tctgcttgtt tcgtttcaat ttgagctgtt ttttcttgtg cgtagcttga
    38521 atgaaccaaa ccaaaagcca acagcatcgt tgcgatatat tttttcattt tattctcctc
    38581 tccacatttc caaaatgtaa ggcaatacat aagggtaagt taaaaagaac atcaccacca
    38641 cagaaactac ccaatacaaa atgaccaggg ttctgcggga gaagtatttg gtgcagatga
    38701 ttttgttgga aaaattcagc aaccagaagc catagccaaa cgcagcgagc gaaatcagca
    38761 acatcggcag ttgtagaaac tccagttggt ttagctccat caaccaaggg gaagagatgc
    38821 caaacactaa ataaaccagt ggtccaatac aacagaatgt ggaggtcacc gccgacacta
    38881 tggcggtcac gcacattgtg ataaatttat ggttataact ctttagagat gaattcatag
    38941 ccaaattctt tcgcctcaaa cgagtttaac gggtcaccac ctacctcagc ataagggtag
    39001 ccaaagttat tcagtggtgt aagttccggt tgtggatata aatcgaaatc acgtccgtga
    39061 ttgaagccta cccagttcat ttcccagttg ccgaataagt atttcgagac cgcttgaaca
    39121 tcagcatcat ccacgtcttt tttctcggct aaacgcattt tagcgacatc ggcagaatct
    39181 acaggcaccc agccgaagcc tgctaaatag aactctgcac ggcagtgctg cccgccgctt
    39241 tcattcgcca cgccatcttt tgagctacca aatgcggttt tggaatattg ccccattttt
    39301 accgcttgcc ctaaacgaat accaaacact tcacgagcag gaatgttaga ggcacgagca
    39361 agtgctacaa acaccgagtt gatgtcggta catttacctt ttagcacttt ggtggtgaga
    39421 attttctcca catcgccatc gccacagcct agcaccgagt tatcacgttc catattatca
    39481 acaatccact tgtggataag ttccgctttt ttcaacggat tttgctcgtt acccacaatt
    39541 ttatcggcat attctttcac aataccgtct gttttaatgt gagccgttgg ttttaaatac
    39601 tccaatacat caacagaata agtgattttc tccggcattt ggtaatttgc caacgcccct
    39661 tgagccatag gctcacgatc ttgggtttgg atcaccattt tcacttttag atcacgcttt
    39721 ggtgcatcag tatcccaagt ggcaaacaag gtttttgcac cataagcgtt attttctgtg
    39781 atataggcgt ttttgtaact tccctcaaac tctatcgatt ttaccgtttg ataatcggta
    39841 tcagacggca acggaatcca caaattagtt gttcctttcg agccttgtgg cacaactaaa
    39901 tcataagatt gcgtaaattc gtaagtgtgg gtagtggtgt ggaaattgtt ctgttgaaca
    39961 ggagcagcaa aagccgtcgc acagcttgaa gccaccaaag aggcaataag tttattcatt
    40021 gaaatgatcc ttattagagt gaaaaaacag ttaaaactta acatattaag tattctaact
    40081 acggattgag gttgcatatt agccaatcga gatgggaaaa gcaatggggg agatgatttt
    40141 ttagatagtg caagcgtcca cgcttgtact agaaacaaag ctaaaataat caataggcac
    40201 aagcgtaaac gcttgcgcca tcagagagaa taacaatcgg tcaaaaatcc tcaaaatttt
    40261 acaaatagat tttttgccct aattttgcac tttcaaatac gctttcgatc acttttagca
    40321 caaaaatgac gtcttcgagt ttgataattg gctcagcatt gtgagcgatg gcttggtaca
    40381 aatcatcaaa caagttttga taactgcctt cggcatttcg atagggctgg cgaaccacat
    40441 taccttccac ttcagtgtgt aaaattcccc agttctcctt ttcttcgata ttccaatctt
    40501 tgccaatagg ttgtacgcca tttttcagca aatcttcttg attatcggga gtttgtttca
    40561 gataagagcc taattttccg tgtaatacaa atgtcgggct tggttcacga gcatatcggc
    40621 tggctttcag caccacttta aaaccgttag cataatagag ctgaatatca aagttatcat
    40681 caaccaatgc cccttcgtgc tgataacgca catcagcata caccgcctcc ggcttcccga
    40741 aaagatacaa agcttggtca attaaatgta cgcctaaatc atacactaaa cctgtaccaa
    40801 gctcgcctga ttctttccat actttaacat ttttctcttt cgcataacgg tcaaaacgaa
    40861 tttcgcaatc taccaactct cccagtaaat tttgctccat tagcgatttt gccgtcgcaa
    40921 tatggctgtc ccaacggcgg ttttggtaca cataaagcac ctttccttgc cgctttgcaa
    40981 gttccgccaa ctcttccgcc tcagccactg ttgccactaa cggtttttca actaaaacgt
    41041 gtttgcctgc aagcaatgac tcttttacca ttgcataatg ggtttgattc ggtgtggtaa
    41101 tcagcactaa atccacttct ggcgttaaaa gagcgttgaa atcccgcact gtttctgcat
    41161 tcggcaacca ttctgccgca ttgttagagg ttcgttcata tacctttaca atattaaaac
    41221 gagcatcttg tttcaaaaac gggatatgaa aaactcggct ggaaaagccg gcacctgcga
    41281 tggcaatatt gatgggttgc atagacaatc ctcataaaat tttgatgtta tctgcttaaa
    41341 taaacaagcg gtcaaatttt aggaaaattt tgcaaaaaac agataaaata cgaccgcttg
    41401 tttaactaat cataggtttt gcaaacaata tctttttctt cacaaaacat ttcactgtgg
    41461 tttgcacggc attgcttttg tacatttaat ttggctctgc ctcggctact atgttcacta
    41521 cgatattcct gagtaaatgg ctttagagta caaatgtgaa cctctttgga atcaagtgga
    41581 tctgagacac caattataat cggcataggg ataccggctt gccccgtagg agtaaccggc
    41641 acgacacagc cacttaatag tatgcttaaa cttattaacc aataacgcat atttttctcc
    41701 ttttaaaatg tataataaat cagaacggag catctttatc gttacagtaa gctcttaata
    41761 tcttgcctaa tttcttggta tttttattaa aatttcggca tttagggcat accaaaagat
    41821 gcaactgcaa acgcatatgt tgttgcaaac gaagtttctc atcacaagac tgtgaaataa
    41881 gttctgttgc ttgcctgcat ttcattttga ttcctctcta aaccaattac gagataaaca
    41941 ctcttgtaaa cgtaaacgag cacgatgaag taatacattt aagttagaga gagaaatttc
    42001 acattcttgg caaatttccg cactactcat ctccaaatgt tcacgcatca taaatactcg
    42061 tgcttgctgg gcgggtaatt tatttaaaca aatatcaaaa attgcccaaa attctttttg
    42121 gtaagttgat tgagctatcc ccctccattc agttacatct tgatgttcca accaatgatc
    42181 gtggcgatcg aaaaaggcac ttggtgattc ttcttcctca catagagaac tgacaggaat
    42241 atgtcgtttt cgtgttctga gtaaatctaa aattttgttc tttaaaatag caaaaatcca
    42301 agttttaagt gcagcttctc gccgaaaaga ggctgaat
//
LOCUS       DANJWC010000002        40924 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.002, whole genome shotgun sequence.
ACCESSION   DANJWC010000002 DANJWC010000000
VERSION     DANJWC010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 40924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40924
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.002"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(76..714)
                     /locus_tag="PWX02_000042"
     CDS             complement(76..714)
                     /locus_tag="PWX02_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDL1728106.1"
                     /translation="MRYTTEQIIEKLRQLKVVPVIALDKAEDILPLAKTLSENGLPVV
                     EITFRSEAAEEAIKLVKANYPEVLIAAGTVLTKEQVLTAKNAGADCIVTPGFNPTIVK
                     YCQVLGLPVTPGVNNPMAIEAALELGIQAVKFFPAEASGGVKMIKALLGPYGNLQIMP
                     TGGISPQNINDYLAIPNVVACGGSWFVEKSLIKEQKWEEIGKLTKEVVELVK"
     gene            complement(802..1711)
                     /locus_tag="PWX02_000043"
                     /pseudo
     CDS             complement(802..1711)
                     /locus_tag="PWX02_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
     gene            complement(1721..3124)
                     /gene="uxaC"
                     /locus_tag="PWX02_000044"
     CDS             complement(1721..3124)
                     /gene="uxaC"
                     /locus_tag="PWX02_000044"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005576058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDL1728107.1"
                     /translation="MKQFLCEDFLLSTPTAQKLYHDYAKDQPIFDYHCHLNPKEIAEN
                     RQFNDLAEIWLEGDHYKWRAMRSAGVEERLITGDADKYSKYLAFAQTVPKCIGNPIYH
                     WTHLELRRPFGITDTLFSPQTAEKIWHQGKALLQQPEFSARGIMQQMNVKLVGTTDDP
                     IDSLEYHKAIAEDSTFDIEVVPSFRPDRAFKIELPLFNDYIEQLGNVADIEINSFDQL
                     KQALLKRIEHFDQQGCKSADHGIEIVRFAAIPDETKLTHILQKRLQNQPLAEEEIAQF
                     STALLVWLGTEYHKRNWVMQMHIGAIRNNNSRMFKLLGADSGFDSIGDRTFAEPLSRL
                     LDSMDQTDQLPKTILYCLNPRDNEMLGSMIGNFQTGGIAGKIQLGSGWWFNDQKDGME
                     RQLQQLSQLGLLSQFVGMLTDSRSFLSYTRHEYFRRILCEMIGGWVERGEAPNDLNLL
                     GNMVKGICYGNAKRYFK"
     gene            complement(3186..4586)
                     /locus_tag="PWX02_000045"
     CDS             complement(3186..4586)
                     /locus_tag="PWX02_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1728108.1"
                     /translation="MSSVQRPFGLKDKLAYMAGDIANDLSFMMSAFFLMLFYTNVLQI
                     EGYVIGILFLVSRIIDAFTDVGMGRLVDTIKPFKEGRFRGLLRRTSPFVCIFGFLMFL
                     HVVKDWAYEAKLIYVCVTYIIWGSFAYTAVNIPYGSMATVISNKPEDRASLSVFRAIG
                     ANIALLFISLCIPLVIYQEVDGKQVIQPEMFTIVMGVFMVIAFLLYQYCWLNTCERVQ
                     LPEKEGRRHNNADCLSDVKSIFTSLFSNRPLMIFILVAIILLLANLLIGTMNPYLYVD
                     YFNNKLGLSIGGMLPVIASFVVAPFAQRIVKRFGKKESAAIALLFTGVVYLALFFLKL
                     ENVWIYLATALIALLGLNYFMVVIWAFITDIIDAQYLKTNKREDGTIYAVYSFARKIG
                     QALAGGLGGFALSYVGYVAKAPSQSQETLNAVYNVATLVPAISCIIIFLLLKFVYPLS
                     KEVVDNNVAELEKRHH"
     gene            complement(4614..6374)
                     /locus_tag="PWX02_000046"
                     /pseudo
     CDS             complement(4614..6374)
                     /locus_tag="PWX02_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609746.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
     gene            complement(6415..7230)
                     /locus_tag="PWX02_000047"
     CDS             complement(6415..7230)
                     /locus_tag="PWX02_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL1728109.1"
                     /translation="MTIFTPKHNFIVCIDSDGCAMDTMNIKHEECFGPSAVKVFDITD
                     RDRFIEIWDRINLYSQTRGINRFKGLVISLEEYGYDKNFSQLKHWVNTTSELSNRSLI
                     QAIQEHPSHDLQLALMWSEKVNIGIKTLHTQDRPFRLVKRSLAIIKEFADIAVVSSAN
                     NEAIIDEWTRHGLLPFVDLVYGQDEGTKTHCLNQLSQYGYQPNHILMVGDSPGDLNSA
                     NEAGVNFFPILCGDEDNSWNQLVLETLGKLVYQKFTLQYQSQLIAKFNANLTA"
     gene            complement(7240..8085)
                     /locus_tag="PWX02_000048"
     CDS             complement(7240..8085)
                     /locus_tag="PWX02_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL1728110.1"
                     /translation="MNVSKNHNFKDKVVVITGAGGVLCAFFAQEIAKTGAKVALLDIN
                     LESAEKYAQAIVQNGGAAKAYKTNVLDLESIQQTRNAINKDFGTCDTLINGAGGNSPK
                     ATTDNEFHEFNLTDSTKSFFELDKSGIEFVFNLNYLGTLLPTQVFAKDMIGKKGASII
                     NVSSMNAYTPLTKIPAYSGAKAAISNFTQWLAVYFSRVGIRCNAIAPGFLVSNQNRGL
                     LFDNEGNPTPRANKILTNTPMGRFGEANELIGGILFLMDENYSSFINGVVLPIDGGFS
                     AYSGV"
     gene            8321..9070
                     /locus_tag="PWX02_000049"
     CDS             8321..9070
                     /locus_tag="PWX02_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HDL1728111.1"
                     /translation="MAAEDLRSYKKIGQELKAELLSGAYKVGDKLPPERDLSNYFNVS
                     RTVIREALIMLELENLIEVRKGSGVYVINLPKSNAEETEELPDVGPFELLQARQLLES
                     SIAEFAAIQATRADVLHLKEILERERRTLEHGDEDYVADEDFHKAIAEITQNEVIIRM
                     QKDLWEYRTNSPMWKGLHSHIPDQSYRTLWLKDHENIIAAIQRKDPVLARKMMWQHLE
                     NVKQKLFELSDLDDPNFDGYLFNVSPLAVNF"
     gene            9089..10273
                     /gene="uxuA"
                     /locus_tag="PWX02_000050"
     CDS             9089..10273
                     /gene="uxuA"
                     /locus_tag="PWX02_000050"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL1728112.1"
                     /translation="MEQAWRWYGPNDPVSLDDVRQAGATGIVTALHHIPNGQVWSVEE
                     INKRKALLAEKGLAWSVVESVPVHEEIKTQTGNYQQWIDNYKQTLRNLASCGIDTVCY
                     NFMPVLDWTRTDLEYEMPDGSKALRFDQVAFAAFELYILKRPGAEATYSSAEQAEAKA
                     YFEQMSDADIAKLTKNIIAGLPGSEEGYTLEEFQAQLDRYQGLSAEKFRTHLAYFLNE
                     IVPVAQEVGIRMAIHPDDPPRPILGLPRIVSTIEDMQWFVETQPLAANGFTFCTGSYG
                     VLAENDLVKMAEQFADRIYFAHLRATQREENPQSFHEADHLAGDVDMFGVVKALLTEE
                     YRRKANGDHRLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLRGLAEFRGLELALKKVYF
                     ND"
     gene            10324..11193
                     /locus_tag="PWX02_000051"
     CDS             10324..11193
                     /locus_tag="PWX02_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL1728113.1"
                     /translation="MFKKYFGEIILFLVTFIAAAGWFFSKHALVGLPPIGFMGGRFIL
                     ASLILLPFSYRELYRLDKVQVKYSVITGLTYGIYTIFWVLGLAFSTVFGGGAFLVSLA
                     MLIAPLLSWLIFKHTPPKIFWLALPIALSGLYFLSAEKGIFHFSLGSTIFLVSSVFGA
                     LYFVLNNQYAKQVSLFPLITIQLAIVGAVCSLYSYFMESWHFPIPTEVWLWFAASVLI
                     ATNLRFLMQTLGQKYCHIGTSALIMLLEPVWTLLLSVMLLGEQLSATKWLGCSLILIA
                     LIIYRLPILRLKK"
     gene            11361..11675
                     /gene="rsfS"
                     /locus_tag="PWX02_000052"
     CDS             11361..11675
                     /gene="rsfS"
                     /locus_tag="PWX02_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDL1728114.1"
                     /translation="MEQNLVEFLTKTLDDLKAQDILAIDVKGKSSITDTMILATGTSS
                     RHVASTAERLITEAKQAGFEVFGDEGKAVADWIVVDFGKAMVHLLQSDSREMYQLEKL
                     WA"
     gene            11752..12219
                     /gene="rlmH"
                     /locus_tag="PWX02_000053"
     CDS             11752..12219
                     /gene="rlmH"
                     /locus_tag="PWX02_000053"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010618862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDL1728115.1"
                     /translation="MKIQLIAVGTKMPAWVTTGFEEYQRRFPKEMPFELVEIPAGKRG
                     KNADTKRILELEGKAMLAAAGKAKIVTLDIPGKAWTTEQLATQLEGWKNDGRDVALLI
                     GGPEGLSPECKAAAEQSWSLSPLTLPHPLVRVVVAESLYRAWSVTINHPYHRE"
     gene            12243..14192
                     /gene="mrdA"
                     /locus_tag="PWX02_000054"
     CDS             12243..14192
                     /gene="mrdA"
                     /locus_tag="PWX02_000054"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDL1728116.1"
                     /translation="MFGKLAKFTASNQREKVRDGQAESNLYARRALVAFLGVLALSGV
                     LLTNLYHLQVVNYEDYQTRSNGNRIKLLPVPPARGLIYDRNGKVLAENLTYFGLFIVP
                     EKTENIEQTLTELKDIVGLTDEDIENYHKEKKRSSRYTPIMLKGNLNEAQMARFAVNQ
                     YRFPSLDVQAYYKRNYPYGELLTHIIGYVAKINENDKKKLQEAGKFGNYAGSHDMGKL
                     GIEKFYEDQLHGVTGFEEVEINNRGKVIRKLRDQAGVAGSSIRLSIDVELQQYVMSLI
                     AKHKGAVVVLDPRDSSILAMVSNPSYDNNLFVGGISGQDYKALLDDPTRPLYSRTTQG
                     TYPPASTIKPFMAVAGLEEGMITPSTTISDPGYWILPGTTQRYRDWKRSGHGATNVNK
                     AITESSDTFFYQLAYKMGIDKMSDWMKKFGFGVKTGIDIFEESAGVMPTREWKQKRYK
                     KPWVQGDTIPVGIGQGYWISTPLQLAKATAVLINNGRVNTPHLMLEVQSSNSVTPYQD
                     PLLYEDIKGVPDVYWNLAKQGMYNVNHAANGTARKVFAGAFYHSAGKTGTAQVFNLNG
                     KNYDKNAIKKELHDHAWFIGYAPYEQPKIVVALILENAGGGGSAAAPVARQIMDYALR
                     KGIGEQPDSQNSEQNKPLVTEEHHE"
     gene            14185..15312
                     /gene="rodA"
                     /locus_tag="PWX02_000055"
     CDS             14185..15312
                     /gene="rodA"
                     /locus_tag="PWX02_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HDL1728117.1"
                     /translation="MNSGFKKLFWKIFSLDLLLLSGLLAITGYGLLVLYSASGANERM
                     FTSRLIQVSLGLGLMLIMAMVPPRFYQKVSPYLYIFCLILLILVDLVGETSKGAQRWL
                     NLGFVRFQPSEIAKLAVPLMVATYLGKRPLPPSFKDTFIALAIIIVPTLLVAIQPDLG
                     TSILVCSAGIFVLFLAGLSWKLIGAGVVFLAGFIPIMWFFLMHDYQKTRVMTLIDPNK
                     DPLGAGYHIIQSKIAIGSGGINGKGWMEGTQSQLEFLPEPHTDFIFAVLSEEHGMIGV
                     LILLAIYLFIIARGLVIGTKSDSAFGRILSGGTALLFFVYVFVNIGMVSGILPVVGVP
                     LPLFSYGGTSYVTLMAAFGLMMSTYVHRKRVGSNNPYDKLK"
     gene            15404..16063
                     /locus_tag="PWX02_000056"
     CDS             15404..16063
                     /locus_tag="PWX02_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HDL1728118.1"
                     /translation="MRLGKIVTLLLAGFLTFSSLNSLAATKNTKAKSSILAKKQATSK
                     AIKKVGISKVKKKEVVAKATKKKTISTVTTKPIKSTKKLARKVVKKTVTAKKKLVKKQ
                     HKPLVKKHFQTGVASYYANYFNGRRTANGETFNNAKMTAAHRTLPFGTLIEVTNLRNG
                     RSVVVRVNDRGPYAHARVLDLSSAAAKKIGMHHSGTARVKIAIVDKSKLGSTNQFYVM
                     N"
     gene            16077..17255
                     /locus_tag="PWX02_000057"
     CDS             16077..17255
                     /locus_tag="PWX02_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDL1728119.1"
                     /translation="MKKTLVKAMSVVGFAVSSIAYADLNTNFNLSAPQINAQTYILMD
                     YNSGEVLVSLNPDQRQYPASLTKMMTSYVVGEALKSGKVQNSDMVIVTENSWAQKFPG
                     SSLMFLDLNSKVSVGELMKGLIIVSGNDASVALAEHVSGSQAAFINEMNKYAQQFGLK
                     NTHFTTVHGLDDPEQYSSARDMAIIGSHIIRDQPEEYKIYAEKEFQYNIKKPQPNRNG
                     LLWDKTIQVDGMKTGHTDKAGYNLVASAINPNTRLISVVMGVPTYKGREVESKKLLQW
                     GFANFETIKSIPAAQSVAEQSVYYGEVNKVQLGSVQDSFVTIPKGRSADLKARYELEK
                     KNLEAPLAKGQVVGKMIYQLDGKDVASTNLQVLQDVPEAGIFGKAWDWIALTVKSLFD
                     "
     gene            17423..17722
                     /locus_tag="PWX02_000058"
     CDS             17423..17722
                     /locus_tag="PWX02_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HDL1728120.1"
                     /translation="MSQPKAINLQDLPQAKLKDLLEFPCHFTFKVVGANREDLVDDVV
                     VVTQKYAKGDYNPRQQKSSKGTYNSVSIDIVAENIEQVETLYTELAKINGVRMVL"
     gene            17784..18437
                     /gene="lipB"
                     /locus_tag="PWX02_000059"
     CDS             17784..18437
                     /gene="lipB"
                     /locus_tag="PWX02_000059"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HDL1728121.1"
                     /translation="MKLIIRQLGIQPYEEIWHQMQSFTDSRDENTDDEIWLVQHPAVF
                     TQGSAGKPEHLLNPTNIPVVQSDRGGQITYHGEGQQIMYVLIDIKRLKSLGKEVSVRD
                     LVTALEQTVVQTLAEYGIESYPKADAPGVYVDGKKICSLGLRIRKGCSFHGLALNINM
                     DLTPFQNINPCGYAGLEMCQLSELVHRPDLRCEEVAPKLVAHFSTILGYNNQQIINN"
     gene            18452..19459
                     /gene="lipA"
                     /locus_tag="PWX02_000060"
     CDS             18452..19459
                     /gene="lipA"
                     /locus_tag="PWX02_000060"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDL1728122.1"
                     /translation="MATSTAQTTATATVKKMSPFKMEKGVKYRDAAKTSVIQVRNIDP
                     DQELLKKPEWMKIKLPANSAKIDSIKNGMRRHGLHSVCEEASCPNLHECFNHGTATFM
                     IMGAICTRRCPFCDVAHGKPLPLDPDEPRKVAETVQDMKLKYVVITSVDRDDLPDRGA
                     AHFSATVREIKALNPHCKVEILVPDFRGRVEQAVAILKENPPDVFNHNLENVPRLYRE
                     VRPGADYKWSLELLKIFKQEFPHIPTKSGLMVGLGETNEEILEVMQDLRDHGVTMLTI
                     GQYLQPSRHHLKVERYVPPAEFDMFREEAEKMGFEHAACGPFVRSSYHADLQAKGELV
                     K"
     gene            19654..20754
                     /locus_tag="PWX02_000061"
     CDS             19654..20754
                     /locus_tag="PWX02_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1728123.1"
                     /translation="MTIKQRICAATLLAFGATANAEIKTITDVLGREVKVDVPAKRVA
                     LTFYYPDYIAVTGVENFDKVVGISREFWEKFNPGSWALFAEKMPNLKNIADLGYVNSG
                     TFSTEKTIALKPDVLVLPEIQYQALASEIPRIEQAGIPVVVVDFNKQTVENHTKSVRV
                     FGQLTGTEERAEKIAKEYEEGIADIQSCIDNAKVSKPKIYAEFGNKGPKEHSFTFGKN
                     MWGAIAETVRGDNISAPFVENWGQINPEQVLVSKPDVIMISGTELGNDTNPEIMSMGI
                     NIEEADAQKRLKGFIERTGWKELPAVKSGRVYGLYHTASRSISDLASAQFMAKALYPE
                     LFKDIDPEKTYLDFHNNYLPITPKGTFFMQLK"
     gene            complement(20816..20995)
                     /locus_tag="PWX02_000062"
     CDS             complement(20816..20995)
                     /locus_tag="PWX02_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728124.1"
                     /translation="MLLSEGKIHAQGRACDVLECDIIQRLYNVNIEILYDGNGSPIIR
                     PLRKCLNIDCCLNRK"
     gene            complement(20995..21600)
                     /locus_tag="PWX02_000063"
     CDS             complement(20995..21600)
                     /locus_tag="PWX02_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1728125.1"
                     /translation="MLNLNDVTIKRGNLVVADKINLHFEQGKIYTLLGPNGAGKSSLL
                     KTLFGEIAHQGTIDFDGNKLTSSHLHSWRKEIGYMPQDTQVEASLSALEVILLGQLDS
                     LNMYISDELLNEAVDIMDKLGISHLAHQDIMRLSGGQRQMVMFAQVLLRKPHILLLDE
                     PVSALDMYHQINLLEYVNAYTRANNLITIMVLHDLSLAAQV"
     gene            complement(21610..22560)
                     /locus_tag="PWX02_000064"
     CDS             complement(21610..22560)
                     /locus_tag="PWX02_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005697612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL1728126.1"
                     /translation="MCLLSLIFDIMTGPSLLPFNEVWKALFQLGEIEETTKVIVYELR
                     LPMAIMALVVGAALGVGGAEIQTLLNNPMASPYTLGLAAAAGFGASIVIAFGGFGLPT
                     EISVPVGAFVMTLFSSAILFAFSSLGRFSSAILVLVGIALLFLFQSLLSLVQFISAPE
                     VSQQILFWLFGNLTKATWQTISIAFGVTTVCILLLFKDAWKLTALRLGEARALSLGIN
                     LTWLRMKTLLIVSVMTATCISFVGIIGFIGLVAPHIARMLLGEDQRFFLPGSMLVGAA
                     FLSMSSVLSKSLVPGALFPVGIITALVGVPFFFWIVLAKR"
     gene            complement(22738..23328)
                     /locus_tag="PWX02_000065"
     CDS             complement(22738..23328)
                     /locus_tag="PWX02_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein phosphodiesterase"
                     /protein_id="HDL1728127.1"
                     /translation="MNFLTHSLISLEIDTQTKQQTLYGNFAGDFYKGLIPHLNLADNL
                     KSGVVLHRSIDKISDRPDSLTTQLLQGTFGRYKGIVSDIMIDHFLAKHFQRLFAKDLN
                     EVQAQIFQQIEQQRTIFPEQFVPMFNWLQSRKALTHYADLDFLEQVFLGMSSRVKKGE
                     ILRNAVTEIWQNYTLLEEAAIQEFFTVKDRATQELS"
     gene            complement(23329..24807)
                     /gene="modF"
                     /locus_tag="PWX02_000066"
     CDS             complement(23329..24807)
                     /gene="modF"
                     /locus_tag="PWX02_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HDL1728128.1"
                     /translation="MQTQPSLTITNARFSLVKQHKLTIDALQIQPNDFWVVVGGNGSG
                     KTAFSLALQDKISLYSGDYGNSFQHIQLLSFEQQQKMLDQIFKDRNNDSVSPDDFGLT
                     ARQVILNGSPKEALCEEYAHKLHIQHLLERPFMHLSTGESRKVLFCQMLVSEPDLFIL
                     DEPFEGLDQQSVQYWQQLIAELQSKMALVLIVNRFNDIPDAANHIALLDNLSLILQGE
                     RAEIEQQAVYSQLKFAEQNVNVPLPESAAPLNSLPENINPFELEKVTIQYGEKKILDN
                     LSWTVEPKQHWWIKGPNGAGKSTLLSIITGDHPQSYANKVRLFGKQRGSGETIWDIKK
                     NIGYVSSQLHMDYRVNCSAMEVILSGFFDSIGVYQQVPNALQLKAMEWLERLNLSNVA
                     KKPFRSLSWGQQRLLLITRAMVKHPSILILDEPLQGLDGINRKLVKQFIEQLVTNSQT
                     QLLFVSHQDSDAPNCITHLFEFVPKGETNEFGFEYHQTALGA"
     gene            complement(24820..25773)
                     /gene="fmt"
                     /locus_tag="PWX02_000067"
     CDS             complement(24820..25773)
                     /gene="fmt"
                     /locus_tag="PWX02_000067"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDL1728129.1"
                     /translation="MSKLNIIFAGTPDFAAQHLQALLDSEHNVIAVYTQPDKPAGRGK
                     KLQASPVKQLAEAHNIPVYQPKSLRKEEAQAELKALNADVMVVVAYGLILPEAVLNAP
                     KYGCLNVHGSLLPRWRGAAPIQRSIWAGDTETGVTIMLMDVGLDTGDMLHKVTTPIEP
                     NETSASLYAKLAELAPLALLEVLNGLANHTFKAEKQDEALSNYAEKLSKEEAKLDWQL
                     PAVQLERNIRAFNPWPFSFLTLTVDGVEQSIKIHQATVLPHQEKAAGTVINVDKTGIQ
                     IATQQGVLNITQLQPASKKPMSVQDFLNGRADWFVSGTQLS"
     gene            25888..26499
                     /locus_tag="PWX02_000068"
     CDS             25888..26499
                     /locus_tag="PWX02_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDL1728130.1"
                     /translation="MEFFIPKADTIFEEEIKKSRFITYLRHTEGMEQAKAFWQELKIQ
                     HPHARHWCWATVAGLPNDSQQYGFSDDGEPSGTAGKPMLNYLLGSGLGEITAVVVRYY
                     GGIQLGTGGLVKAYGNGVQQALLQLERIRKVLRKNYRLQCEYEQFNTISHLISEKDIV
                     IVEQLFSDKVDLLLAFNSSEVEAFADELTQRFSGKLALIKEEI"
     gene            26503..27987
                     /locus_tag="PWX02_000069"
     CDS             26503..27987
                     /locus_tag="PWX02_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter"
                     /protein_id="HDL1728131.1"
                     /translation="MQFFSIIRIVGILVMCFSFTMLVPATVALIYGDGGGRAFLEAFA
                     LNFMVGTLLWWLCRDNKNKLRSREGFLIVVLFWVVLGLLGAVPFIILENPDLNVAESI
                     FESFSGLTTTGATVITGLDSLPKAILFYRQFLQWLGGMGIIVLAVAIIPLLGIGGMQL
                     YRAEMPGPLKEQKMRPRIAETAKALWLIYLSLTILCAVAFWLAGMTVFDAISHSFSTV
                     SIGGFSTHDASIGYFNNAAISYITVFFLLLSACNFALHFAVIDRIRDAQSRKKGGILS
                     RLYWGDYEFRFFLIVQLGLFLVCTLVLVGYGYFDNTSVTIEQALFQSVSISTTAGFTT
                     NDFSQWSSFLPTLLVLSSFIGGCAGSTGGGLKVIRVLLLYLQSKREIHRYIHPNVIQP
                     IKLGNHVLSERVVDGIWAFFSAYFFVFVICWLATIACGMETFDALNAVIASLNNLGPA
                     LGSVSSNFTQVPDSAKLVLTFAMVCGRLEVFTLLVILSPVFWKD"
     gene            27988..28515
                     /gene="hemG"
                     /locus_tag="PWX02_000070"
     CDS             27988..28515
                     /gene="hemG"
                     /locus_tag="PWX02_000070"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HDL1728132.1"
                     /translation="MKTLILYFTTDGQTKKIAERLADVITHEVELISLKDQAVDFAEK
                     IANADQIVIGASIRYGHFNPLVYQFVEQHYPALNAKKTAFYGVNLTARKDNRNTPETN
                     TYVRKFLAKVKWKPTHIEVIAGALLYLRYKPFDRLMIRFIMKITKGETDTSKEYEYTD
                     WQQVENFGKLLANQL"
     gene            28648..29043
                     /gene="mscL"
                     /locus_tag="PWX02_000071"
     CDS             28648..29043
                     /gene="mscL"
                     /locus_tag="PWX02_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDL1728133.1"
                     /translation="MSILKEFREFAVKGNVVDMAVGVIIGGAFGKIVSSLVSDVVMPP
                     IGWLIGGVDFKDLAIEIAPAKEGAEAVMLKYGAFIQNVFDFLIIALAVFGMVKVINSL
                     KKAPVVEEAPAEPTAEEKLLTEIRDLLKK"
     gene            complement(29156..29419)
                     /gene="rpsT"
                     /locus_tag="PWX02_000072"
     CDS             complement(29156..29419)
                     /gene="rpsT"
                     /locus_tag="PWX02_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDL1728134.1"
                     /translation="MANIKSAKKRAVQSEKRRQHNASQRSMMRTFIKKVYAAVTAGDK
                     AAAQTAFVEMQKVVDRMASKNLIHANKAANHKSKLAAQIKKLA"
     gene            29683..31257
                     /gene="murJ"
                     /locus_tag="PWX02_000073"
     CDS             29683..31257
                     /gene="murJ"
                     /locus_tag="PWX02_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDL1728135.1"
                     /translation="MSKKLLRSGMIVSFMTLISRILGLIRDIVVATILGTGVSADIFL
                     FANRIPNFLRRLFAEGAFSKAFVPVLAEYNADNDPNKTREFIAKVSGTLGGLVTVVTL
                     VAMIASPVVAALFGTGWFLDWLYDGPNAEKFTQASFLLKITFPYLWFITFVALSGAVL
                     NTIGKFGVMAFSPVLLNVAIIAVALWGRDYFDSPDIALAWGVFLGGLLQFLFQIPFMK
                     KEGLLVKPKWAWKDEGVTKVRKLMIPALFGVSVTQLNLLLNQVIASFLITGSISWMYY
                     ADRLIEFPLGLFGIAISTVVLPSLSRIAKNKELTEIQRGEHFQNTMDWGVRMVLLLGI
                     PAMIGMAVLAQPIIMTMFMRGKFGFEDVLATSYPLWVMCLGLNSYMLISVLANGFYAN
                     QNTKTPVKVGIIAALSNICFGLAFAPFLGYIGLALASACSALVNVSLLYVNLSKNGYY
                     KVSHKTVLFVLKLFIAACVMGALVAYFTPEIELWALMTMWQKVYWLIWLMILAGGSYF
                     ASILVLGIRKKDFRSA"
     gene            31259..31726
                     /locus_tag="PWX02_000074"
     CDS             31259..31726
                     /locus_tag="PWX02_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HDL1728136.1"
                     /translation="MKSLKLKIPPPAWFLLCAALMWLVAQYVPVSLPNYQHTAIFFSG
                     LAVAIIGGAVAVSALWVIHQAKTTYSPFRPQHTAKLVTWGIYRKSRNPMYLSLLLGLI
                     AWALWLGAMLAWLVLPLFVWLITYFQIKPEEQILAQKFGTHYAKYCNQVRRWL"
     gene            complement(31791..33800)
                     /locus_tag="PWX02_000075"
     CDS             complement(31791..33800)
                     /locus_tag="PWX02_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDL1728137.1"
                     /translation="MDLIKKLNTGSTFRIPIFLPSLLFVSFVAVFCIIFPQQAQTSLD
                     TIKNSLFQHFSWFYIFAGSIFFLFLIFLSFSRLGDIKLGADTDEPEFGFGSWIAMLFA
                     AGMGIGLMYFGVAEPILHYLKPVQQNLTEPERMKEAMMTTFYHWGIHAWAIYGVIALA
                     LAYFGFRYKLALTIRSGFYPLLKHRISGFWGHLIDIIALCSTIFGLTTTLGFGVMQVS
                     AGFNNLGLIEQSNFTVLAIIVTVAMALAVLSAVSGVGKGVKILSEINLTLAGLLLIFV
                     IITGPTLLLFSSFTENLGYYFSSLLEMSFRTFAYEPEHQGWLSGWTVLYWAWWASWAP
                     FVGLFIAKISKGRTIREFILGVLFVPSLFNILWMTSFGSSAIWFEQQTAGALAEVSGN
                     TEQLLFTFFEQLPFGSIASFVAVIVISIFFITSADSGIFVLNSIASQGEENAPKWQSV
                     LWGALLAILALSLLYSGGLASLQTMTLIIALPFTFIMLILCIGLWKGLMVDNQYFNKK
                     FSQGSQHWAGKDWKQRLEKIINPSNKQDVRHFFIKVARPAFLELIEEFESYGLIAKMN
                     FTNEQNPKLEFEVVKENLRNFIYGIESVPRELSDLVVGDDNLPNIEQNTIYEPITYFL
                     DGRKGYDVQYMTKEELIADVLQQYERFINLAMDNSHDLMTADFNH"
     gene            33992..34531
                     /locus_tag="PWX02_000076"
     CDS             33992..34531
                     /locus_tag="PWX02_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1728138.1"
                     /translation="MHTQHNAQKMVLPILLLISLSHLLNDLVQAVMVSIYPMLKENYQ
                     LSFAQIGMISLIYQITASIIQPGIGLYTDKYPKPNLLPVSMLITLLSVILLAFSLNFG
                     VLLIAAALMGIGSAIFHPEASRVARMASGGKFGTAQSLFQVGGNSGSALGPLLVALFI
                     VPLGQSAVIWVVAFVLLAL"
     gene            34559..35170
                     /locus_tag="PWX02_000077"
     CDS             34559..35170
                     /locus_tag="PWX02_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1728139.1"
                     /translation="MKYEAKPLIISPNHIQQRLHGKLLARALLIIALLMLAKFIYIAS
                     LSNYFTFYLMEKFQLNIAMAQLSLFTFLGVVALGTFVGGPIGDKIGRNAVIWFSFLGM
                     APFALLLLHADLFWTIVCAIFAGFIMSSAFSAMVVYAQEAVSGRVGLISGTMFGLMFG
                     IGGLSAAVLGFLADEYGLESIFKACAYLPLLGFAALGLPKRNI"
     gene            complement(35181..36071)
                     /gene="rarD"
                     /locus_tag="PWX02_000078"
     CDS             complement(35181..36071)
                     /gene="rarD"
                     /locus_tag="PWX02_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDL1728140.1"
                     /translation="MNISGKLQGWHYALLCYLTWGTFPIYWYPINQSGMPAEQILAQR
                     VLWAALFAVFLLLIFQQGRAVINAFKQPKVLGLFVLSSFMIGVNWLVYLWAIVNEHIL
                     DASLGYFINPIFNVFVGRLILKEQLNKAQLLALLFAISGILWLAIPAGQIPWVALILA
                     ASFCIYGLIRKLAPMEPLSGLALETLLMSPFAITYLCFCYHQNILVFSELNPLQMAVL
                     LCSGIATTLPLLWFAMGARRISMSLLGMLQYISPTLQFLCGVLIFNETLSAERAVGYI
                     LVWIGVGVFLLGMKKAGVKR"
     gene            complement(36153..37136)
                     /gene="epmA"
                     /locus_tag="PWX02_000079"
     CDS             complement(36153..37136)
                     /gene="epmA"
                     /locus_tag="PWX02_000079"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDL1728141.1"
                     /translation="MNNLQLDNIDWQPTASIPHLIQRSKIITEIRKFFTDRGLLEVET
                     PILSEFSVTDVHLSTFNTQFQSPFSPEAKTLHLMTSPEYHMKRLLAAGSGPIFQLCKV
                     FRNEEAGKRHNPEFTMLEWYRPHFDMYRLINEVDDLLQQILDCEPAESFSYQFVFQTY
                     VGLDPLSATKAQLVEKARKHGLQCDDDEQRDTLLQFLFSEVVEANIGQERPTAVYHFP
                     STQAALAQISSEDHRVAERFEFYYKGLELCNGFHELDDADEQIHRFEQDNLQREKMGL
                     EPQQLDVRFLAALKAGFPNCSGVALGVDRLLMLAMDAEKIEEVISFGIEKA"
     gene            37468..39270
                     /gene="frdA"
                     /locus_tag="PWX02_000080"
     CDS             37468..39270
                     /gene="frdA"
                     /locus_tag="PWX02_000080"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDL1728142.1"
                     /translation="MQSVNFDVAIIGAGGGGLRAAIAAAEANPNLKIALISKVYPMRS
                     HTVAAEGGSAAVIKDTDSFDNHFNDTVGGGDWLCEQDIVEYFVEHSPIEMTQLERWGC
                     PWSRREDGEVNVRRFGGMKIERTWFAADKTGFHILHTLFQTSIKYPNIVRFDEHFVLD
                     ILTDNGEARGCVAMNMMEGTLVQINANAVVIATGGGCRTYRFNTNGGIVTGDGLSMVY
                     RHGVALRDMEFVQYHPTGLPNTGILMTEGCRGEGGILVNKDGYRYLQDYGLGPETPIG
                     KPENKYMELGPRDKVSQAFWQEWRKGNTLKTAKGVDVVHLDLRHLGEKYLLERLPFIC
                     ELAKAYEGVDPAKAPIPVRPVVHYTMGGIEVDMNAETSIKGLFAVGECASSGLHGANR
                     LGSNSLAELVVFGKVAGENAARRALEVTARNQPQIDAQAKDVVARLHALANQEGNESW
                     SDIRNQMGDAMEEGCGIYRTQESMEATVNKIQELKERYKNISVKDKSSVFNTDLLYKI
                     ELGFILDVAQSIACSAVERKESRGAHQRLDYVERDDVNYLKHTQAYYNAEGTPTIKYS
                     DVKITKSQPAKRVYGAEAEAQEKVKKAAEAQAQQ"
     gene            39286..40017
                     /locus_tag="PWX02_000081"
     CDS             39286..40017
                     /locus_tag="PWX02_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HDL1728143.1"
                     /translation="MANLDKMTIEVLRYNPESDSEPHLDRYEVPYDSQTSLLDALGFI
                     KDELEPELSYRWSCRMAICGSCGMMVNGKPKLACKTFLRDYSGFMRIEPLANFPIERD
                     LVVDLSHFIDSIEAIKPYIIDNKAPEGQRTKQTPAQLEKYRQFSMCINCGLCYAACPQ
                     FGLNPEFIGPAAITLAHRYNLDNRDNGREERMKLLSSKNGVWSCTFVGYCSEVCPKHV
                     GPASAVNQGKLESAKDYVISMIKPR"
     gene            40027..40419
                     /gene="frdC"
                     /locus_tag="PWX02_000082"
     CDS             40027..40419
                     /gene="frdC"
                     /locus_tag="PWX02_000082"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDL1728144.1"
                     /translation="MSATKRRPYVRGMKASWWKKLDFYKMYMVREATCLPTVWFCIVL
                     FYGAVALSKGTFATDFVDFLQNPLVVILNIISLAAMLYHAATLFVMTPAVMSVMVKGK
                     HLPVSVGRNILWAATGVISVIALILVYI"
     gene            40433..40780
                     /gene="frdD"
                     /locus_tag="PWX02_000083"
     CDS             40433..40780
                     /gene="frdD"
                     /locus_tag="PWX02_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDL1728145.1"
                     /translation="MSLDQNPKRSNEPPVWLLFSAGGMVSALFFPVVIFIIGLLLPFG
                     LVSPDNIISFAHTWIGKLAIMVLAIFPMWAGMHRIHHGLHDLKVHTPAGNVIFYGLSI
                     LYTILVIIAVVQI"
BASE COUNT        11816 a         7992 c         9054 g        12062 t
ORIGIN      
        1 tgttatgctt tgtaaataag aggaaagaga tctattttgg gtagaaaata gctcattgct
       61 tggaacttta aatggttatt tcaccaactc aaccacttcc ttcgtcaatt tcccaatctc
      121 ctcccacttc tgttccttaa tcagggattt ctccacaaac caagagccgc cgcaggcgac
      181 tacgttggga atggcgaggt agtcgttgat gttttgtggc gagatgccgc cggtcggcat
      241 aatctgtaag ttgccgtaag ggccgagcag ggctttaatc attttcacgc cgccactggc
      301 ttcggcaggg aaaaatttaa ctgcttgtat gccaagttcg agggcggctt caatcgccat
      361 cgggttgttc acacccggtg taacaggcaa acctaacact tggcaatact taacgatagt
      421 cggattaaaa cccggagtca caatgcaatc cgctccagcg tttttggcag ttaagacttg
      481 ctctttggtc agcactgtgc ctgctgcaat caatacttcc ggatagttgg ctttcactaa
      541 tttaattgcc tcttcagctg cttctgagcg gaaagtgatt tccaccaccg gcaaaccgtt
      601 ttcggaaagg gttttcgcca gcggtaaaat atcttctgct ttatccaaag caatcacggg
      661 aacaactttc agttgtctta atttttcaat gatttgttcg gtggtgtaac gcatttctag
      721 cctcattatg ttgtattgca agcggtcgct tttgtaaaaa attttgcaaa tttaaccgct
      781 tgtcgttgtg ctattgttgg tctaaatgga ttgttcaaac tgcgatttca aatgagtagt
      841 ggcggatttg gcaataatcg cccctttgtg ctgaatgacg ataccggcaa cggcatttcc
      901 ttgttggcaa caggtaacga catctttacc caataagtaa ccgttaagga agccggcatt
      961 aaaggaatcg ccggcggaag tggtatcgac caccttctct accgcaacag ttggcacaaa
     1021 agtacgctcc ccgttttgca acaccgttgc ccccagagag ccttgtttca ccacaatggt
     1081 atgaatgcca tattgagcca gacggtcaaa ggtggcttgc tcattcatat cgccccaaag
     1141 cagttgctcg tcatcaaagg tcacgagagc taaatcgact aattgcaaca gctcggcata
     1201 gtgagcttgg gcagcagcca cactttccca cagtttcggc cggtaattgc tgtcaaaggc
     1261 gattttcacc ccttgtgttt tgagctcacg caactgtgca atcagcaaag tgcggtcgtt
     1321 ttccggcaag atggcaaggc tgataccgct caagtaaacc atatccacct cgtgtaacgc
     1381 cgagagtgct tttgaatagt cggggtgttg caacaaataa cgggcggcag attgatttcg
     1441 ccaatacagg aatgtccgct cgccctgctt atccaactga atcagataca gccccggctg
     1501 gcggtgcttg tcacgctgcc aacatacctt gactgagttt atccgtacca agagcagaaa
     1561 tataacgcac ctcaatctgt tcaggcgaac tcacccgagc caaataggta gcggtgttga
     1621 gagaatcacc gccgtaggtt tgtcgcatat tgccaaaggg ttcgccgttg agttcgatca
     1681 tacattcgcc aattagagcg atttttttca ttgttatctc ctatttgaaa taacgttttg
     1741 cattaccgta gcaaatacct tttaccatat tgccaagtaa attcagatcg ttcggtgctt
     1801 caccccgttc aacccagcca ccaatcattt cgcaaagaat acggcggaag tattcgtggc
     1861 gggtgtaaga taagaaacta cgggaatcgg ttagcatacc gacaaattgg ctaagcaaac
     1921 ctaattgtga aagctgttgc agttggcgtt ccattccatc tttctgatca ttgaaccacc
     1981 agcctgaacc caattggatt ttgccagcaa tgccgccggt ttggaaattg ccgatcatac
     2041 taccgagcat ttcgttatca cgcggattta agcaatataa aatggttttc ggaagttgat
     2101 cagtttgatc catactgtct aataaacggg agagtggctc ggcaaaggtg cggtcgccaa
     2161 tggagtcaaa tccgctgtct gccccgagta acttaaacat acggctgtta ttattgcgga
     2221 ttgccccaat gtgcatttgc atcacccagt tgcgtttgtg gtattccgtg cctaaccaga
     2281 ctaataatgc cgtgctgaat tgtgcgattt cctcttctgc aagcggttga ttttgcaatc
     2341 ttttttgcaa aatatgggta agttttgttt catcaggaat agcggcaaaa cgtacgattt
     2401 caatgccgtg atcggcggat ttacacccct gctgatcgaa atgttcaatc cgttttagta
     2461 gtgcttgctt taattgatcg aagctgttga tttcaatatc cgcaacgtta ccgagctgct
     2521 caatgtagtc attaaacagc ggcagttcga ttttaaatgc tcggtcaggt cggaaacttg
     2581 gcacaacctc aatgtcaaag gtgctatctt ccgcaatcgc tttgtggtat tccaaagaat
     2641 caatcgggtc atcagtagtt ccgactaatt tcacattcat ctgttgcata atgccacggg
     2701 cggaaaattc aggctgttgc agtaatgctt tgccttgatg ccaaattttt tctgcagttt
     2761 gtggcgaaaa cagcgtgtca gtaatcccaa acggacggcg aagttctaaa tgcgtccaat
     2821 ggtaaatcgg gttgccgata cattttggca cagtttgtgc aaaggctaag tatttactgt
     2881 atttatcagc atcgcctgta attaaacgct cttccacacc ggcagaacgc attgcccgcc
     2941 atttgtagtg atcgccttcc agccagattt cggctaaatc attaaattgg cgattttccg
     3001 caatttcttt tgggtttaaa tggcagtggt agtcaaaaat cggctgatct ttggcatagt
     3061 cgtgatagag cttttgtgcc gttggagttg agagtaaaaa gtcttcacat aaaaattgtt
     3121 tcatatttag ctcgagattg attggtaaca aagtaggggt aggtgttatt gcctacccca
     3181 ataggttaat ggtgacgttt ttctaattct gcgacattgt tatcgaccac ttctttggag
     3241 agtggataaa caaatttcag cagcaggaaa ataataatac aagagattgc cggcactagt
     3301 gtggcaacat tatagaccgc atttaaagtc tcctgacttt ggcttggtgc tttggcgaca
     3361 tagccgacat aacttaatgc gaaaccaccg agaccaccag caagcgcttg cccgattttg
     3421 cgtgcaaacg agtaaaccgc ataaatcgta ccgtcttctc gtttatttgt tttaagatat
     3481 tgcgcatcaa taatatccgt aatgaaagcc cagatcacaa ccataaagta gtttaaaccc
     3541 agcaatgcaa tcagagcggt agctaaatag atccaaacat tttccaattt taggaaaaag
     3601 agagcgagat aaactacacc ggtgaagagt agcgcgattg cggcagactc ttttttgcca
     3661 aagcgtttga caatacgttg agcaaagggg gctactacaa atgaggcgat cacaggcagc
     3721 attcctccga tagataatcc gagtttgtta ttaaagtaat caacgtagag atagggattc
     3781 attgtgccga tcagtagatt tgccaataac agaataatag caactaaaat gaagatcatt
     3841 aacggtctat tactaaatag gctggtaaag atactcttta catcggataa acaatcagca
     3901 ttgttgtggc gtcttccttc cttttcaggt aactgcaccc gttcacaggt attgagccag
     3961 cagtattgat agaggagaaa tgcgatcacc ataaacactc ccatcacaat agtaaacatt
     4021 tcaggctgaa tgacctgttt accatcaact tcttggtaaa ttaccaatgg gatacacaat
     4081 gagataaata gtagggcaat gttagcacct attgcacgaa atacggaaag tgatgcgcga
     4141 tcttctggtt tgttgctgat aacagtggcc attgagccat aaggaatatt tactgctgta
     4201 tatgcaaagc ttccccaaat aatgtaggtc acacatacat agataagttt tgcttcatat
     4261 gcccaatctt taacgacgtg taaaaacatt aaaaagccaa agatacaaac aaatggcgat
     4321 gttctgcgta acagccctct gaaccgccct tctttgaaag gcttaattgt atcaaccaat
     4381 cgccccatac caacatcagt aaaagcatcg atgatacgag agactaaaaa taaaataccg
     4441 ataacatagc cttcgatttg taatacattg gtataaaaca gcattaagaa gaatgcagac
     4501 atcataaagc tgaggtcatt ggcaatatct cctgccatat aagcaagttt atcctttaga
     4561 ccaaaaggtc tttgtactga actcatgaag aactccttat taggaataat ggattaaata
     4621 tgggtatctt tataaccaca gaatgcatca actgggctaa cgcctttaaa ctcagaatta
     4681 cccattaatt tttcgactag cagttctatt gttggctctt taccatcata agcattgatg
     4741 taagttttta cttgtggaac atcaactaaa tggaatgggc attgtacgga aacgaaaatt
     4801 gtaggtactt catgcacata aaatggaata tcaggtgttc ctttggtagc ttgccataca
     4861 atacgctgaa cagtactcat ttgaacattt gcaacattga taatgagatc atagtgatcg
     4921 gttaatgttg taattgggcg tttttgcgag tattcattac ggatggtatt cacttgctct
     4981 tcatctgaca ttttgaggat attatccata ggtgatgtat aaatagagac cgtaaaccct
     5041 ttttctgcca gtttttcttt taggatttct gttgcgctac gttggctacc tgcaaccatt
     5101 ttaccgaatc caccatctgt gcctttgaca ttcacaagta agactcttgg aaaacgctca
     5161 acattaatag gtaaaatatc ttgtttattc ttcactaatg tgattgcttt atctgcgaca
     5221 tcacggaaga tcactttatg ttctggtaaa cctatttttg ccagtgcttg ttctttaggt
     5281 tcaagtaatg tttctttcgg gcgattatgt aatcctaatt ttgcttttaa tcctagaata
     5341 cgggtaagag catcgtttag acgttcttct gtaataatgc cgtttttata gccggccatc
     5401 atataatcaa aatcttcatc aggatcatta aagaaaagga ataaatcgca accagctgca
     5461 atcgctttcg gcagcatttc actgcgtttc atagcggatg tcattgcaac catatgactt
     5521 gcatctgtta caacaacccc gttaaaacct aatttacctc gtaataaatc agttaaaatt
     5581 ggctttgaaa gtgttgcggg taaacagtct tcatacgata aattagggtt aaaatgttgc
     5641 tcgtaagctg gtagatggat atgaccagcc attattgagg gtaaaccggc atcaattaga
     5701 ccttgataga cttttccaaa agagttatct cactcttcac aactgaagct gtttacgcta
     5761 aaagaaaggt gttgatctct ttcatcgacc ccatctccag ggaaatgttt ggctgttggc
     5821 gcaattccgc tttcttgaat gcctttcata taagctaagg acatttccag tactttatct
     5881 ggattagagc caaaaacccg gttagaaata attggatttc tccagttata gctaatatca
     5941 acgataggtg caaaactcca gttacagcca atagcggctg cctcaatacc tgctacacgc
     6001 cccatttcat aagcatattt ggcatcacct gttgcaccaa ttttgacctg taaaccaatt
     6061 tccgttccat cactacaagc accgttacct ccagcttcag tgttagccgc aattaataga
     6121 gggattttgc ttttggtttg taaaatatag ttttggttat aaacttcttc agccggacct
     6181 gggttataac gtactgcacc aatatggtag cggtttaaaa tatcagtgag atattcctct
     6241 gttctactcg atcccatatt cacaaaaagt tgtcctattt tttcttctag agtcatatta
     6301 gcgatagtgc tgtttaccca ttgaatgtct tcatctttta agtaataagg tttttttgtt
     6361 aaatcaacag acataatttc tcctatttac gcttcaaaac cgtgaaatac gggatcaagc
     6421 ggttagattc gcattgaatt ttgcaattaa ttgagattga tattgaagag tgaatttttg
     6481 gtaaaccaat tttcctaatg tctctaatac caactgattc caggagttat cttcatctcc
     6541 acataatata gggaagaaat tgaccccagc ttcattggca ctatttaaat ccccaggaga
     6601 atctccaacc attaaaatat gatttggctg atatccatat tgtgataatt ggtttaaaca
     6661 gtgagttttt gttccttcat cttgcccata aacaagatca acaaaaggca gtaaaccatg
     6721 ccgtgtccac tcatcaataa ttgcttcatt atttgcagaa cttactacgg cgatatcagc
     6781 aaattcttta ataatagcaa gagagcgttt tactaaacga aatggtctat cttgtgtatg
     6841 tagagtttta atcccaatat tgactttttc tgaccacatc aatgcaagtt gaagatcatg
     6901 agaagggtgc tcttgtatag cttgaataag cgagcggtta gataattcag aagttgtatt
     6961 tacccaatgt tttaattggc tgaaattttt gtcataccca tactcttcca gagaaataac
     7021 caacccttta aatcggttga tgccgcgtgt ttgagagtac agattgattc tatcccaaat
     7081 ttcaataaaa cgatctctat cggtaatgtc aaaaacttta acagcagagg ggccaaaaca
     7141 ctcctcatgc ttaatgttca ttgtatccat tgcacatcca tccgaatcaa tgcatacaat
     7201 aaagttgtgc ttaggtgtaa atattgtcat gagattctcc tatactccac tataagcgga
     7261 gaaacctcca tcaatcggta ataccacacc atttatgaag ctggaataat tttcatccat
     7321 taagaataaa attcccccaa tcaattcatt cgcttcacca aaacgtccca ttggggtatt
     7381 agttaaaatt ttattcgctc taggagtagg gtttccttca ttatcgaaaa gtaaccctct
     7441 attttggttg ctaactagaa agccaggggc aattgcatta cagcggattc ctaccctaga
     7501 gaaataaacg gcaagccact gagtaaaatt actgatggct gctttagctc cagagtaagc
     7561 aggaatttta gtaagagggg tgtaggcatt catgcttgat acattgatga tactggctcc
     7621 ttttttacca atcatatctt ttgcaaatac ctgagtaggt agcaatgtac ctaaatagtt
     7681 gagattaaat acgaattcaa tgccactttt atctaattca aagaatgatt ttgtactatc
     7741 agttaagtta aattcatgaa attcattatc ggtggtggct ttaggactat tacctcctgc
     7801 accattaatt aatgtgtcac aagtaccaaa gtctttgttg atggcattgc gagtttgctg
     7861 aatactttct aaatcaagca cattggtttt ataagctttg gctgctcctc cattttgcac
     7921 aatagcctgt gcatattttt ctgctgattc aaggttaata tctaatagag ctactttagc
     7981 acccgttttt gctatttctt gtgcaaaaaa tgcacagagc acacctccag ctcccgtaat
     8041 aacgacaact ttatctttga aattatggtt ttttgaaaca ttcatattgc ttatctccta
     8101 tcaggggtga ttaaagtatg gcaaatatac taccactttt acaactggta aaccaattgt
     8161 tagtaatttt gtgatataga tcacaaaatc acgacacact ggtttacctc ttgttaaatt
     8221 taactaaaat atgctttaat ttactgttgt ttcttggtct tttagtatgt aatgtgctat
     8281 tttggtaaac ctttttaaaa tactatagag aaacaaaata atggcagcgg aagatctacg
     8341 ctcttataaa aaaatcgggc aagaattaaa agcagaatta ttaagtggtg catacaaagt
     8401 aggggataaa ttaccgccgg agcgtgattt atctaattac tttaatgtca gccgtaccgt
     8461 tattcgtgaa gcactgatta tgcttgagct ggaaaattta attgaagtgc gtaaaggctc
     8521 cggtgtttat gtgattaatt tacccaaatc aaatgctgag gaaacagaag agttgcctga
     8581 tgttggtcct tttgaactgt tgcaagcaag gcagctatta gaaagtagta ttgccgagtt
     8641 tgctgctatt caagccactc gggcagatgt tttacactta aaagagattt tagagcgtga
     8701 acgcaggacc ttagaacacg gtgatgaaga ttatgttgca gatgaagatt ttcataaagc
     8761 gattgctgaa ataacgcaaa atgaagtgat tatccgtatg caaaaagatt tatgggagta
     8821 tcgaaccaat agtccgatgt ggaaagggtt acattcacat attcccgatc aaagttaccg
     8881 cacgttatgg ttaaaagatc acgaaaatat tattgcggca atacagcgaa aagatcctgt
     8941 attggctcga aaaatgatgt ggcagcattt ggaaaatgta aaacaaaagc tgtttgaatt
     9001 atcggattta gacgatccga attttgatgg ttatctgttt aatgttagcc ctttggctgt
     9061 taatttttaa ttagatgagg agtttactat ggaacaagca tggcgttggt atggaccaaa
     9121 cgatccagta tctttagatg atgtaagaca agcaggggca acggggattg tgacagcgtt
     9181 gcaccatatt cctaatgggc aggtgtggtc ggttgaggaa attaataaac gtaaggcatt
     9241 attagctgaa aaagggctgg cttggtcggt ggtagaaagt gtgcctgttc acgaagaaat
     9301 caagactcaa accggcaact atcagcaatg gattgataac tataagcaaa ccttgcgtaa
     9361 tttggcaagc tgcggcattg atacggtgtg ctataacttt atgccggtgt tagattggac
     9421 tcgtaccgat ctggagtatg aaatgcctga cggctcaaaa gcattgcgtt ttgaccaagt
     9481 ggcgtttgcg gcgtttgagt tatatatctt aaaacgcccg ggagcggaag ccacttactc
     9541 atcagcagaa caagccgaag ccaaagccta ttttgagcag atgagtgacg ctgatattgc
     9601 aaaattaacc aaaaatatca ttgccggttt gccggggtct gaggaaggct atacgctcga
     9661 agaattccaa gcacagcttg accgctatca agggctctcg gctgaaaaat tccgtaccca
     9721 tttagcctat ttcttaaatg agattgtgcc ggttgcacaa gaggtgggca ttcgtatggc
     9781 gattcaccct gacgatccgc ctcgcccgat tttaggcttg ccacgcattg tttcaaccat
     9841 agaagatatg caatggtttg tggaaactca accgcttgca gccaatggct tcacgttctg
     9901 taccggttca tacggcgtat tagccgagaa tgatttggtc aaaatggcag agcagtttgc
     9961 agatcgcatt tattttgctc acttacgagc tacccaacgt gaagaaaatc cacaaagttt
    10021 ccacgaagcg gatcatcttg ccggtgatgt cgatatgttt ggcgtggtga aagcattact
    10081 taccgaagag tatcgccgca aggcaaatgg cgatcaccgc ttaattccaa tgcgtccgga
    10141 tcacggccat caaatgctcg atgatctgaa gaaaaaaacc aacccaggct attctgcgat
    10201 tggtcgttta agaggattag cggagttccg tggattggag ttggcactta agaaagtcta
    10261 ttttaacgat tagccttatt tattataatg ccctctactg tagagggcat tttatagttt
    10321 gatatgttca aaaaatattt cggcgaaata atcctttttc tcgttacctt tattgctgca
    10381 gcggggtggt ttttctctaa acacgcttta gttggattac cgcctattgg ctttatggga
    10441 gggcgtttta tattagcctc tttgattctt ctgccttttt cttaccgaga gctgtatcgt
    10501 ttagataagg ttcaagttaa atattcggtg attaccggtc ttacttacgg aatttatact
    10561 attttttggg tattggggct ggcttttagt acagtgtttg gtggaggggc ttttttggtg
    10621 agccttgcga tgctgattgc tcctttgctc tcttggttga tattcaaaca tacgccaccg
    10681 aagatttttt ggttagcctt acccattgca ttaagtgggc tttactttct ttctgcggag
    10741 aaaggcatct ttcatttttc cttaggcagt acgatttttc tggtgtcatc agtgtttggg
    10801 gcgttatatt ttgtgttaaa taatcaatac gccaaacaag tctcattgtt tccattaatt
    10861 actatccaat tggcgatagt cggtgcggtc tgtagcttgt attcctattt tatggaaagt
    10921 tggcatttcc cgattcccac cgaagtgtgg ctctggtttg ccgcaagtgt attgattgcg
    10981 acaaatttac gttttttaat gcaaacactg gggcaaaaat attgccatat tggtacttct
    11041 gcactcatta tgttacttga gccggtttgg acattgttat tgagcgtgat gttattgggg
    11101 gaacaactta gtgcgacaaa atggctaggt tgcagcctga ttttgatagc attgattatt
    11161 tatcgactcc ctattctacg actcaaaaaa taggtaaaac aaaagcaaat atgccatagt
    11221 attggaagca gttaggcatt ttagagtaag taacaagcgg tcatttttgc aaaaaatctt
    11281 gcaaaaatga ccgctttctt tcttttatcc gttcaatcta tgttaaaatt ctacgttttt
    11341 ataagatgag agagagaaac gtggaacaga atttagtcga atttttaacc aaaaccttag
    11401 atgatttaaa ggcacaagat attttagcca ttgatgtgaa agggaaatct agcattactg
    11461 atacgatgat tttagccacc ggtacatcga gccgtcacgt tgcttcaacg gcagagcgtt
    11521 taattacgga agcaaaacag gctgggttcg aggtatttgg agatgaaggt aaagcagttg
    11581 ccgattggat cgtggtcgat tttgggaagg caatggttca cttgttgcaa tccgatagcc
    11641 gtgagatgta ccaattagag aaactttggg cgtagcagtt caataaggaa cgacaagcgg
    11701 tgatttttgc aacatttttt gcaaattaaa ccgcttgtaa gtgaaagaga gatgaaaatt
    11761 caattaattg cggttggcac taaaatgccg gcgtgggtaa cgacagggtt tgaagagtat
    11821 caacgccgtt ttccgaaaga gatgccgttt gagttggtgg aaattccggc aggaaaacgg
    11881 ggcaagaatg ctgatactaa acgcattctt gagcttgaag gcaaagcaat gttggcggca
    11941 gcgggcaagg caaaaatcgt gactttggat attccgggca aagcgtggac aaccgagcag
    12001 ttagccactc agttagaggg ctggaaaaac gatgggcgag atgtagcgtt attgattggc
    12061 ggcccggaag gattatcacc ggaatgcaaa gccgctgccg agcagagttg gtcgttgtcg
    12121 cctttaacct tacctcaccc actggtgcgg gtggtggtgg cggaaagttt gtatcgggcg
    12181 tggtcggtta ccattaatca cccttatcat cgagagtagt gagtcatttt agatagtgtt
    12241 atatgtttgg taaattagca aaattcacag cttcaaacca gcgagaaaag gtgcgagacg
    12301 ggcaggcaga aagcaatctg tatgcccgcc gtgccttagt tgcgtttctg ggggtgttgg
    12361 cgttaagtgg tgttttatta accaacttgt atcacttgca agtggtgaat tatgaggact
    12421 accaaacccg ttcaaacggt aaccgcatta aattgttgcc tgtgccaccg gctcgaggct
    12481 taatttatga ccgtaacggt aaagtattgg cggaaaattt aacctatttc gggctgttta
    12541 ttgtgcctga aaagacggaa aacattgagc agactttaac cgagttgaag gatattgttg
    12601 ggctgacgga cgaagatatc gagaattatc ataaagagaa aaaacgcagt tctcgctata
    12661 ccccaattat gctcaaaggc aatttgaatg aggctcagat ggctcgtttt gcggtgaacc
    12721 aataccgttt tccaagttta gatgtgcagg cgtattataa gcggaattat ccttacggcg
    12781 agcttttaac ccatattatc ggttatgtgg cgaaaattaa cgaaaacgat aagaaaaagc
    12841 tgcaagaagc cgggaaattc ggcaattatg ccggttcgca cgatatgggg aaattgggga
    12901 ttgaaaaatt ctatgaagat cagctccacg gtgtcaccgg ttttgaagag gtagaaatta
    12961 ataaccgagg gaaagtaatc cgtaaattgc gtgaccaagc aggggttgcc ggtagcagta
    13021 ttcgcttgag tattgatgtg gaattacagc aatatgtgat gtcgttaatt gctaagcata
    13081 aaggggcggt ggtggtgctt gaccctcgtg atagcagtat tttagcgatg gtctcaaacc
    13141 caagttatga caataattta tttgtgggcg gtatttcagg gcaggattat aaagcattat
    13201 tggacgatcc aacccgacca ctttatagcc gtactacaca aggcacttat ccaccggctt
    13261 caactattaa gccatttatg gcggtggcag gtttggaaga gggaatgatt accccttcta
    13321 ccaccatttc agaccccggc tattggattt tgcccggcac tacacaacgt tatcgagatt
    13381 ggaaacgtag cggacacggt gcaactaatg tgaataaagc aattaccgaa tcgtctgata
    13441 cctttttcta tcaattagcc tataaaatgg ggattgataa aatgtcggat tggatgaaaa
    13501 aattcggttt cggagtgaaa accggtatcg atatttttga ggaatccgca ggggtaatgc
    13561 caacccgtga atggaaacaa aaacgctata aaaaaccttg ggtgcagggc gatactattc
    13621 cggttgggat tgggcagggc tactggattt cgaccccgtt acagttggcg aaagccacgg
    13681 cggtgttaat caacaacggg cgggtgaata caccgcattt aatgcttgaa gtgcaaagca
    13741 gtaattcggt tacgccgtat caagacccat tgttgtatga agatattaaa ggcgtgccgg
    13801 atgtctattg gaatttagca aaacagggaa tgtataacgt aaaccacgcc gcaaacggta
    13861 cggcacgaaa agtgtttgcc ggtgcatttt atcattcagc agggaaaacc ggtacggctc
    13921 aggtgtttaa cttaaacggt aaaaattacg ataaaaatgc gattaaaaaa gagttgcacg
    13981 accacgcttg gtttatcggc tatgctcctt atgaacaacc taaaatagtg gtggcattaa
    14041 ttttagaaaa tgcaggcggt ggtggtagtg ccgcggctcc tgttgctcgt cagattatgg
    14101 attatgcttt gagaaaaggc attggtgaac agccggattc gcaaaattct gagcaaaata
    14161 aaccgcttgt cacagaggaa catcatgaat agtggcttta aaaaactttt ctggaaaatt
    14221 ttttcactcg atctcctttt attatcagga ttattagcta ttaccggtta tggtttatta
    14281 gtactatata gcgcaagcgg tgcaaatgag cggatgttta ccagtagact gattcaagtt
    14341 tcgctagggc ttgggctaat gttgataatg gcaatggtac ctcctcgctt ttatcaaaaa
    14401 gtttcgcctt atctgtatat tttctgtctg atattattga ttttggtgga tttggtcggc
    14461 gaaaccagta aaggagcgca acgttggttg aatttaggat ttgttcgctt tcagccttct
    14521 gagattgcca agctcgctgt gccactgatg gtagccactt atttaggtaa gcggccgctt
    14581 cctccgagtt ttaaagatac ttttatcgcc ttagccatta ttattgtgcc aaccttattg
    14641 gtggcaattc agcccgattt gggaacatct attttagtgt gttctgccgg tatttttgtt
    14701 ttatttttgg cagggctaag ttggaaatta attggcgcag gtgtggtgtt tttagccggt
    14761 tttatcccga taatgtggtt tttcttaatg cacgattatc aaaaaacccg ggtgatgacc
    14821 ttaattgatc ctaataaaga cccgttgggt gccggctatc atattattca atccaaaatt
    14881 gcgataggct ctggtggcat taatggaaaa ggctggatgg aaggaacgca atcccagctt
    14941 gaatttttac ctgaaccgca taccgatttt atttttgcag tgttaagcga agagcatggt
    15001 atgataggtg tgctgatttt attagcgatt tatttgttta ttattgcccg aggtttagtg
    15061 attggcacaa aatcggattc tgctttcggg cgtatattat caggtggtac agcccttcta
    15121 ttttttgttt acgtttttgt aaatattggt atggtgagtg gtattttacc cgtagtcggt
    15181 gtaccattac cgcttttcag ttacggcgga acctcctatg tgacgctgat ggcggcattt
    15241 ggtttgatga tgtcaaccta tgttcaccgt aagcgtgtag gtagtaataa tccttatgat
    15301 aaactgaaat aaattaaact tttgcttttt tgcggtatca aaaagcagtg aaacgatcat
    15361 cgttacaaac aataacgaca tccacataga ggaaaaaact tatatgagat taggtaaaat
    15421 tgtaaccctg ttgttagctg gatttctgac atttagttca ttaaattctt tggctgcgac
    15481 aaagaataca aaagctaaat cgagtatttt agccaagaag caagcaactt ctaaagcaat
    15541 aaaaaaagta ggtatttcta aagttaagaa aaaagaagta gtggctaaag ctacaaaaaa
    15601 gaaaacaatt tctactgtta caactaagcc gattaaatct acaaaaaagc tggctcgcaa
    15661 ggttgtcaag aaaacggtaa ccgctaagaa aaaattggta aaaaagcagc ataagccact
    15721 ggtcaaaaaa catttccaga ctggtgttgc cagctattat gcaaattact ttaatggtcg
    15781 ccgaaccgct aatggggaga ctttcaataa cgcaaaaatg acggctgcac atcgaacttt
    15841 accatttggt actttaattg aagtgacgaa tttacgaaat ggtcgttctg tggtggtgcg
    15901 tgtaaatgat cgcggacctt atgctcacgc acgggtcttg gatttatcat cagccgcagc
    15961 gaaaaaaatc ggtatgcacc attcggggac ggcaagagtg aaaattgcga ttgttgataa
    16021 gtcaaaattg ggatcgacta atcagtttta tgtaatgaat taatttaaga gggaatatga
    16081 aaaaaacatt agtgaaagcg atgagcgtgg taggttttgc ggtttcaagc attgcctatg
    16141 cggatttaaa cactaacttc aatttatcgg cgccccagat caacgcacaa acctatattt
    16201 tgatggatta caactccggt gaagttttag tgagcctaaa tccggatcaa cgtcaatatc
    16261 ctgcatcatt gaccaaaatg atgactagct acgtagttgg tgaagcccta aaatcgggaa
    16321 aagtgcaaaa cagtgatatg gtgatagtca ccgaaaattc gtgggcgcaa aaattccccg
    16381 gctcttcatt aatgttttta gatttaaaca gcaaagtttc agtaggtgag ttgatgaaag
    16441 ggctgattat cgtctcgggt aatgatgcct ctgtggcgct tgctgaacat gtttcaggct
    16501 cacaagcggc gttcattaat gaaatgaaca aatatgccca acagtttggc ttaaagaata
    16561 ctcactttac cactgttcac ggtttagatg accctgagca atattcttct gctcgtgata
    16621 tggcgattat tggttcgcat attattcgtg atcagccgga agagtataaa atttatgcgg
    16681 aaaaagagtt ccaatataac atcaaaaaac cacaaccaaa ccgcaacgga ttattgtggg
    16741 ataaaaccat tcaagttgat ggtatgaaaa cagggcatac tgataaagcc ggctataact
    16801 tagtcgcatc agcaattaac ccgaatacac gtttaatttc tgtagtgatg ggcgtgccaa
    16861 cttataaagg gcgtgaagtg gaaagtaaga aattactgca atggggcttt gcgaactttg
    16921 aaaccattaa atctattccg gcagctcaat cggtggcaga gcaaagtgtc tattacggtg
    16981 aagtcaataa agtacaatta ggctcggtgc aagatagctt tgtgactatc ccgaaaggtc
    17041 gctcggcaga tttaaaagcc cgttatgagt tagagaagaa aaacctcgaa gctcctttag
    17101 caaaagggca ggtagtgggt aaaatgatct accaacttga cggcaaagat gttgcaagca
    17161 ctaacttaca agtcttacaa gatgtaccgg aagccggtat tttcggcaaa gcgtgggatt
    17221 ggattgccct tactgtgaag agcttgttcg attaatcttg ttatttgcct ttttatcccc
    17281 atctagggag caatgcaagc ggtgtgattt tgcaaaaaat ttgcaaaatt tcaccgctta
    17341 ccgttttaga tatgaatgta taaaaagtgt agtggcgaaa aatttttcgc cccccgaaga
    17401 aagaatagaa taaggatcaa aaatgtctca acccaaagcg ataaacttac aagatttacc
    17461 tcaagccaaa ttaaaagatc tgctagagtt tccgtgtcat ttcacgttca aagtggtggg
    17521 agcaaaccga gaagatttgg ttgatgatgt ggtagtggtc acgcaaaaat atgccaaagg
    17581 cgattacaat ccacgtcagc aaaaaagctc aaaaggtacc tataattccg tttcgattga
    17641 tattgtggcg gaaaatattg agcaagtaga aactctctac acggaacttg ccaaaattaa
    17701 tggcgtaaga atggtattat aatgcgaggg gcgaatgaca ttcgccctca atttttcaat
    17761 aaagcgatgt tggaaaatag aaaatgaaat taataattcg ccaactaggc attcagcctt
    17821 atgaagaaat ttggcaccaa atgcaatctt tcacagatag ccgtgatgag aataccgatg
    17881 atgagatctg gctggttcag cacccggctg tgtttacgca aggatcggca ggcaaaccgg
    17941 agcatttgtt aaatccaacc aatattccgg tggtgcaaag cgaccgtggc ggtcaaatta
    18001 cttaccacgg cgaggggcag caaattatgt atgtgctgat tgatatcaaa cggctgaaat
    18061 ccttgggtaa agaagtgtcg gttcgagatt tagtgactgc ccttgagcaa accgtggtac
    18121 aaacgttggc agaatatggc atagaaagtt atccaaaagc cgatgcaccc ggggtgtatg
    18181 tggacggcaa gaaaatctgc tcacttggtt tacgaattcg aaaaggttgc tctttccacg
    18241 gtttggcatt aaatatcaat atggacttaa cgccttttca aaacatcaat ccgtgcggtt
    18301 atgcagggct ggaaatgtgc caattaagtg aactggttca tcgccctgat ttgcgttgcg
    18361 aagaggttgc tccaaaatta gtggctcatt tcagcacaat tttaggctat aataaccaac
    18421 aaattattaa caattaggat aaaacacagt aatggctaca tcgacagctc aaacgacggc
    18481 aactgctaca gtgaaaaaaa tgagcccttt caaaatggaa aaaggggtga aatatcgtga
    18541 tgccgccaaa acctctgtga ttcaggtgcg taatatcgac ccggatcaag aactcttaaa
    18601 aaaaccggag tggatgaaaa tcaaactgcc ggcaaattcg gcaaaaattg atagcattaa
    18661 aaacggtatg cgtcgccacg gcttgcactc ggtatgcgag gaagcctctt gcccgaattt
    18721 acacgaatgt tttaaccacg gtactgcaac ctttatgatt atgggggcaa tctgtacccg
    18781 tcgttgcccg ttctgtgatg tggcacacgg caaaccgctt ccgcttgacc cggacgaacc
    18841 tcgcaaagtg gcagaaacgg tgcaagatat gaaactcaaa tatgtggtga ttacctcagt
    18901 ggatcgtgat gatttgcccg accgtggtgc ggctcatttc tctgcgaccg tgcgtgaaat
    18961 caaagcctta aatccgcatt gtaaggttga aattttagtg cctgatttcc gtggacgtgt
    19021 ggagcaagcg gtggcaattc tcaaagaaaa tccacctgat gtctttaacc acaacctaga
    19081 aaatgtgcca cgtctctatc gtgaagttcg tccgggagcg gattacaaat ggtcgttgga
    19141 attattgaaa atcttcaagc aagagttccc gcatattccg accaaatccg gcttaatggt
    19201 aggcttaggt gaaaccaacg aagaaatctt ggaagtaatg caagatttac gagaccacgg
    19261 cgtgacgatg ctcaccatcg ggcaatactt acaaccaagc cgccaccact taaaagtgga
    19321 acgttatgtg ccaccggcag agtttgatat gttccgtgag gaagctgaaa aaatgggctt
    19381 tgaacacgca gcctgcggac cgttcgtgcg ttcttcttac cacgcggatt tacaagcgaa
    19441 aggcgaattg gtgaaataaa atcgataagg gcgaatatta tttgcccttt gtttttgtgg
    19501 tttgcaaagt tttagcaaaa ttaaaccgct tgtaatctct tctaatttac tcttctatag
    19561 ataatttcgt tgttgcgaat tattttcatt gactatttgt tatagaaagt tacaattcaa
    19621 ctttccattt ctttttggag ttccaatgaa aaaattacta ttaaacagcg tatttgtgct
    19681 gccacattgc ttgcttttgg tgcaactgcc aatgccgaaa ttaaaaccat cactgatgta
    19741 ttagggcgtg aggtcaaagt tgatgtgcct gctaaacgtg ttgcactgac tttctattac
    19801 cctgactata ttgcggtaac cggtgttgaa aatttcgata aggtagtagg gatttcacga
    19861 gagttttggg aaaagttcaa cccaggcagt tgggcattat ttgcagaaaa aatgccaaat
    19921 ctaaaaaaca ttgccgatct tggctatgtg aatagtggta ctttttcaac agaaaaaacc
    19981 atcgcattaa aaccagatgt gttagtgcta cctgaaattc aatatcaagc gttagctagt
    20041 gaaattccac gcattgagca agctggcatt cctgttgtag tagtagattt caacaagcaa
    20101 actgttgaaa accatacaaa aagcgtgaga gttttcggtc agcttacagg cactgaagag
    20161 agagcggaga aaattgccaa agagtatgaa gagggcattg ctgatattca gagctgtatt
    20221 gataatgcaa aagtaagtaa gccgaaaatc tatgctgaat ttggtaataa aggcccgaaa
    20281 gagcatagtt ttacctttgg taaaaatatg tggggagcga ttgccgaaac ggtgcgaggc
    20341 gataacatta gtgccccgtt tgtagaaaac tgggggcaga ttaaccctga gcaggtgtta
    20401 gtttctaaac ctgatgtgat tatgatttca ggtacggaac taggcaacga cactaaccct
    20461 gagattatgt cgatggggat taatattgaa gaagccgatg ctcaaaaacg cttaaaaggc
    20521 tttattgagc gtacaggttg gaaagagtta cctgcggtaa aatcagggcg tgtttatggt
    20581 ttatatcata cagcttctcg ctcaatttca gatttagcga gtgctcaatt tatggcaaaa
    20641 gcactttatc ctgaattgtt taaagatatc gatcctgaaa aaacgtattt ggatttccac
    20701 aacaactact taccaattac gccaaaaggc acattcttta tgcaactgaa ataaaaacaa
    20761 caagcgatca ttttgcagat ttattttgca aaatgaccgc ttgtattcgc ttttactatt
    20821 tgcggttcaa acagcaatca atatttaaac atttgcgaag cgggcggata atcggcgagc
    20881 cgtttccatc atataaaatc tcaatattga cattatagag tctctgaata atatcgcatt
    20941 ccaacacatc acaagctctg ccttgtgcgt gaattttccc ctcgcttaat agcatcacac
    21001 ttgagccgca aggcttaaat cgtgcagcac cataattgta attaggttat tcgctcgagt
    21061 gtaggcattc acatattcaa gtaagttgat ttgatgatac atatccaacg cactgacagg
    21121 ctcatctagc agcagaatgt gtggtttacg tagcaatact tgtgcgaaca ttaccatttg
    21181 ccgttgcccg ccacttagcc gcataatatc ttgatgagct agatgtgaaa taccgagttt
    21241 atccataata tcaacggctt cattgagtaa ttcatcgcta atatacatat tcaggctatc
    21301 aagttgccct aataaaataa cctctaaggc actgagtgag gcctctacct gtgtgtcttg
    21361 tggcatatag ccaatttctt tacgccaact atgcaagtgg cttgaggtta atttattacc
    21421 gtcaaaatca attgtgcctt gatgagcaat ttccccaaat agagttttaa ggagagaaga
    21481 tttacccgca ccatttgggc caagtaaagt atagatcttg ccttgttcaa aatgtagatt
    21541 gattttatca gccaccacta agtttccacg cttaatagtt acatcgttta agttgagcat
    21601 agaaaatcct tatcgtttcg ccaatacaat ccagaaaaag aacggcacac cgactaatgc
    21661 cgtaataata ccgaccggaa ataatgcgcc cggcacaagg ctttttgata gcacggatga
    21721 catcgataaa aatgccgcac ccactaacat tgaaccgggc aggaaaaagc gttggtcttc
    21781 gcctagcaac attcgggcaa tatgcggggc aactaagcca ataaagccga taatgcctac
    21841 aaagctaatg caggtggctg tcattacaga cacaatcagc aatgttttca ttcgtaacca
    21901 agttaagtta atgcctaagc tcaatgctcg agcttcgccc aaacgcaagg cagttaattt
    21961 ccacgcatct ttgaataata ataaaataca gactgtagtt acgccaaagg caattgagat
    22021 agtctgccaa gtagctttag ttaaattacc gaatagccaa aacagaattt gttgggatac
    22081 ctcaggtgca gaaataaatt gcaccaatga cagtaaagac tgaaataaaa acaataaggc
    22141 aattcctacc agcactaata ttgccgagct aaaacgtcct aaagaggaaa aggcaaataa
    22201 aatggcggaa gaaaatagtg tcatcacaaa agccccgaca ggtacagaaa tttcggtggg
    22261 taaaccaaat ccaccaaagg caatcacaat cgaagcacca aagccggctg cagctgctaa
    22321 acccaatgta taagggcttg ccattggatt attaagcaaa gtttgaattt cagctccgcc
    22381 aacaccaagt gctgctccaa ccaccaatgc cattattgcc attggtaagc gtagctcata
    22441 gacaatcact ttggtggttt cttcaatttc gcctaattgg aataaggctt tccaaacttc
    22501 attaaacgga agcagggaag ggcctgtcat aatatcgaaa atgaggctga gtaaacaaat
    22561 gagtaagaaa aagagtaata aaaaactgcg ttcttttcaa tagttcggtg ctttttagct
    22621 atttgttggg gagagagcgt tgtttgcata aaaatgtaat gtttgagaat tattatcaga
    22681 caggataaga gaaaagatcg ataaattcaa gaatatccaa gataccgact aaagtattca
    22741 actaagttct tgagtagccc tgtcttttac agtaaaaaac tcctgaatag cggcttcttc
    22801 taaaagagta taattctgcc aaatttcggt tacagcattt cttaaaatct cacctttctt
    22861 aactcttgat gacattccca agaatacctg ttctaagaaa tctaaatcgg cataatgagt
    22921 taatgctttt cgtgactgta accagttaaa catcggcaca aattgttcag gaaaaatcgt
    22981 gcgttgttgt tcgatttgct gaaagatctg tgcttgtacc tcatttaaat ctttagcaaa
    23041 taaccgttgg aaatgtttag cgagaaaatg gtcgatcata atatccgaca caatgccttt
    23101 ataacgcccg aatgtgcctt ggagtaattg ggttgtgagg ctgtccggtc ggtccgaaat
    23161 cttatcaatc gagcggtgta acaccacacc gctttttaag ttatccgcta aattgagatg
    23221 cggaattaaa cctttataga aatcaccggc aaagttgcca tatagggtct gttgcttggt
    23281 ttgggtgtca atttcaagtg aaatcagcga atgggtaaga aaattcattt atgctccaag
    23341 tgcagtttgg tgatactcaa acccaaactc gttggtttct cctttcggta caaactcaaa
    23401 taaatgcgta atgcaatttg gagcatcgct gtcttggtgc gaaacaaaaa gcagttgcgt
    23461 ttggctgtta gtcactaatt gttcaatgaa ctgtttgact aatttgcggt taatgccgtc
    23521 taaaccttgt aacggttcgt ctagaatcag aattgatggg tgtttcacca ttgctcgggt
    23581 aatcaaaagt aagcgttgtt gcccccacga gagagagcga aacggctttt tggcaacatt
    23641 gcttaaattg aggcgttcca accattccat tgcttttaat tgcaaggcat tcggcacttg
    23701 ttgatacacg ccgatgctgt caaaaaagcc agaaagaatg acctccattg cggagcaatt
    23761 tacccgataa tccatatgca actggctgct gacatagccg atatttttct taatatccca
    23821 aatggtttcg ccgcttcccc gttgtttgcc aaatagtcgg actttattgg cataagattg
    23881 tgggtgatcg ccggtgataa tcgaaagcaa ggtcgatttc ccagctccat tcgggccttt
    23941 aatccaccaa tgttgttttg gctcaaccgt ccaactgagg ttgtctaaaa ttttcttttc
    24001 gccgtattgg atagtcacct tttccagctc aaacggattg atgttttcgg gcagactgtt
    24061 cagtggagcg gcactttcag gcaaaggaac gttcacattc tgttctgcaa attttagctg
    24121 agaatagacc gcttgttgtt caatttctgc tcgctctcct tgcagaatta atgataaatt
    24181 atctaataac gcaatatgat tggcagcatc aggaatatcg ttaaaacgat ttacaatcag
    24241 cactaacgcc attttgcttt gtagttcggc aatcagttgt tgccaatatt gcaccgactg
    24301 ctgatccagc ccttcaaacg gttcgtctaa aatgaataaa tccggctcgc tcactaacat
    24361 ttggcaaaat agcactttgc ggctttcacc ggtggaaagg tgcataaacg ggcgttcaag
    24421 cagatgttga atatgcaatt tgtgggcata ttcctcacat aatgcctctt ttggactgcc
    24481 atttaaaatt acttgtcgtg cggttaagcc gaaatcatcg ggcgagacac tatcgttgtt
    24541 ccgatctttg aaaatctgat ccaacatttt ttgttgttgc tcgaaagaga ggagctgaat
    24601 atgttggaac gaattaccat agtcgccgga atagagcgag attttatcct gtaaagcgag
    24661 tgaaaaagct gttttgcctg aaccgtttcc gcctacgacc acccaaaagt cattgggttg
    24721 gatctgtaag gcgtcaatcg tgagtttatg ttgtttaacc agtgaaaatc gggcattggt
    24781 tattgtaagt gaaggctgtg tttgcatata tttcccgttt taacttagtt gtgtcccgct
    24841 gacgaaccaa tccgctcgac cgtttaagaa atcttgcacc gacattggtt ttttactcgc
    24901 cggttgaagt tgggtaatat tcagcacgcc ctgttgggtg gcaatttgaa tcccggtttt
    24961 atccacatta attaccgtgc cggctgcttt ttcttggtga ggtaacaccg tggcttggtg
    25021 aattttgata ctttgctcaa cgccatcaac ggttaaggtt aaaaaactaa acggccaagg
    25081 gttaaaagct cggatattgc gctccagttg aacggctggg agttgccaat ccaatttagc
    25141 ttcttctttg gaaagtttct cggcgtaatt ggacagtgct tcatcttgtt tttccgcttt
    25201 gaatgtgtgg ttggctaaac cgtttaacac ctcaagcaat gccagagggg caagttctgc
    25261 cagtttggca tacaaggagg cggaggtttc attcggctca atcggggttg tgactttatg
    25321 taacatatca ccggtgtcta agccaacgtc cattagcata atggttacgc cggtttctgt
    25381 atcacctgcc caaatcgaac gctgaatcgg agctgctcca cgccagcgag gtagtagcga
    25441 accgtgtacg tttaggcaac catatttagg agcattcaat actgcttcag gtagaattaa
    25501 gccgtaagct accaccacca tcacatcggc atttagggct ttcagttcag cttgagcctc
    25561 ttccttacgc agggatttgg gttggtaaac aggaatgttg tgagcttcgg ccagctgttt
    25621 taccgggctg gcttgcagtt ttttgcctcg cccggcaggt ttatccggct gagtataaac
    25681 cgcaatcacg ttatgctcgg aatctaataa ggcttgtaaa tgttgagcgg caaaatcagg
    25741 cgtgccggca aaaataatat tgagttttga catatttttt attcgttcaa gttatcaatg
    25801 aaagacttat taaaccatta aacagcacaa ataaaaagtg gcttgaatat ggtatcattg
    25861 ccacattcaa gataagagaa tcaaacaatg gaatttttta tccccaaagc ggatacaatt
    25921 tttgaagaag agattaaaaa aagccgattt atcacctacc ttcgccatac tgagggtatg
    25981 gagcaggcca aggcattttg gcaggagctg aaaattcagc atccgcacgc acgccattgg
    26041 tgttgggcaa cggtggcagg cttgccgaat gattcgcaac aatacggctt ttcggatgat
    26101 ggagaaccgt ccggtacagc aggtaagcca atgctcaatt atttgcttgg atcaggcttg
    26161 ggggaaatta cagcggtggt ggttcgctac tacggtggta tccagcttgg caccggcggc
    26221 ttggtgaaag cctatggcaa tggggtgcaa caggcgttat tgcagttgga gcggattcgc
    26281 aaagttttgc gaaaaaacta ccgcttgcaa tgtgaatatg agcagtttaa taccatttct
    26341 catctcatta gcgagaaaga tattgtgatt gtcgagcaac tttttagcga taaagtggat
    26401 ttgttgctgg catttaactc gagcgaggtt gaggcctttg cagatgaatt aacccaacgc
    26461 ttttcgggca agctcgcatt aataaaagaa gaaatctagt ctatgcaatt tttttccatt
    26521 attcgtattg ttggcatttt ggtgatgtgt ttttcattca caatgcttgt gccggcaacg
    26581 gtcgccctaa tttatggcga tggcggtggg cgggcgtttt tagaagcctt cgctctgaat
    26641 tttatggtcg gtacgctgct ttggtggctg tgccgtgaca acaaaaacaa gctacgttcc
    26701 cgtgagggct ttttgattgt agtactgttt tgggtggtgc tgggtttgct cggggctgtg
    26761 ccttttatta ttttggaaaa ccccgattta aacgtggcgg aatccatttt tgaatccttt
    26821 tccggcttaa ctacaacggg tgcaaccgtc atcaccggct tagatagctt gcccaaagcg
    26881 attttattct accgccaatt tttgcaatgg ctagggggaa tggggatcat tgtgttagcg
    26941 gttgcgatta ttccgctgtt aggcattggt ggaatgcaac tttaccgtgc ggaaatgccc
    27001 ggaccgctca aagagcagaa aatgcgacct cgcattgcgg aaacggcaaa agcgttgtgg
    27061 ctgatttatc tcagcctgac gattctgtgt gcggtcgcat tttggctcgc cggaatgacg
    27121 gtgtttgatg ccatttcgca tagcttttcc acggtttcga ttggcggatt ttctacccac
    27181 gatgccagca tcggctattt taataatgcg gcaattagct atattaccgt gtttttcctg
    27241 ctactttctg cctgcaactt tgcattgcat tttgcggtga ttgatcgcat acgagatgca
    27301 caatcacgca aaaaaggggg tattttaagc cgcttgtatt ggggcgatta cgagtttcgt
    27361 tttttcttga tagtgcagtt agggcttttc ttggtttgta ccttggtgtt agtgggttac
    27421 ggttatttcg ataatacctc tgttacgatt gagcaagcgt tgtttcaatc ggtgtccatt
    27481 tccaccacgg ccggctttac taccaatgat tttagccaat ggagttcgtt tttacccacg
    27541 ctgttggtgc tgtcttcatt tatcggtggt tgtgccggct caaccggtgg cggcttgaaa
    27601 gtgattcggg tgttgctgct ttatttacaa agtaaacgtg aaatccaccg ctacattcac
    27661 cccaatgtga ttcaaccgat taaattaggc aaccacgttt tatcggaacg ggtggttgat
    27721 ggtatttggg cgtttttctc ggcgtacttt ttcgttttcg tgatctgctg gcttgctacc
    27781 atcgcttgcg gaatggaaac ctttgatgct ctaaatgcgg taattgcctc actaaataat
    27841 ctaggtcctg cattagggag cgtgagcagc aatttcaccc aagtgccgga cagtgccaag
    27901 ttagtgctga cttttgcaat ggtatgcgga cgtctggaag tgtttacctt gctggtgatt
    27961 ttaagccctg ttttttggaa agattaaatg aaaaccttaa ttctctattt tacgaccgat
    28021 ggtcaaacca aaaaaattgc cgaacgattg gcggacgtga ttactcacga agtcgagctg
    28081 atttcgctaa aagatcaagc ggtcgatttt gccgaaaaaa ttgcaaatgc cgaccaaatt
    28141 gtcatcggtg cttcaattcg ctatgggcat tttaacccgt tggtttatca gtttgtggaa
    28201 cagcattatc cggcattaaa cgccaagaaa actgcctttt acggtgtgaa tttgaccgct
    28261 cgcaaagata atcgaaacac gcctgaaacc aatacttatg tgcgtaaatt tttagctaaa
    28321 gtgaaatgga aaccaaccca cattgaggta attgctgggg cattacttta cctacgctat
    28381 aagccattcg accgattgat gattcgcttt attatgaaaa ttaccaaagg cgaaaccgat
    28441 acctccaaag aatatgagta caccgattgg cagcaggtgg agaattttgg caaattatta
    28501 gccaatcagt tataatcaaa ctgatctaaa tcaaaaaaat gtaaatttat ttaaatgttg
    28561 tagaaatttt aaagattttg attataatcg ccagcccacg ttcttgcgaa gaccgtgttt
    28621 aatcattgaa tgaaaggaaa gccccctatg agtattttaa aagagttccg tgaatttgcg
    28681 gtaaaaggta atgttgttga tatggctgtc ggtgtgatta tcggcggtgc attcggtaaa
    28741 attgtttctt cattagttag cgatgttgta atgccaccta tcggttggtt aatcggtggc
    28801 gtggatttca aagatttagc cattgaaatc gccccggcaa aagaaggtgc ggaagcagtg
    28861 atgttaaaat acggtgcatt tatccaaaac gtatttgatt tcttaattat cgcacttgcg
    28921 gtattcggta tggtgaaagt gattaactca ctgaaaaaag ctccagttgt agaagaagct
    28981 cctgcagagc caacagcgga agagaaatta ttaactgaaa tccgtgattt attgaaaaaa
    29041 taattaactg attttgaaac aagggctatt ttagaatatc tagaatagcc ctttgtgtta
    29101 tggttaaaaa gaaaaaaacc gacataaagt cggttttttt catatctgtt cgatattacg
    29161 ctaatttttt gatttgcgct gctaatttag atttgtggtt agccgctttg ttagcgtgga
    29221 ttaagttttt agatgccata cggtctacaa ctttttgcat ttcaacgaaa gccgtttgag
    29281 ctgctgcttt atcgcctgct gttactgctg catatacttt tttgatgaat gtacgcatca
    29341 ttgagcgttg gcttgcgttg tgttggcggc gtttttcaga ttgaaccgca cgtttttttg
    29401 ctgacttgat attagccaag gtcaaactcc taaaatatct gtgtaaaaat tagatataaa
    29461 caaaatcgtt gtttatattg cctatcgata gtttttaatt cattttcaac gagacacgtg
    29521 cttgaaaata acaccgtttt tctgatagaa aacgataggg cgagatttta acagtttttt
    29581 tctgattttc ctataacaaa ctgctaaaat tatgatcgat ttttaggatc agactacaaa
    29641 cggtctgatt tttcaataat tttactaata ttactcttta ttatgagcaa aaaactttta
    29701 agatcaggaa tgatcgtaag ctttatgacc ttaatttctc gcatactcgg cttgattcga
    29761 gacattgtgg tggcaaccat tttaggcaca ggcgtgagtg ccgatatttt cctgtttgcc
    29821 aaccgtattc ccaattttct tcgccgtttg tttgccgaag gggcattttc taaagccttt
    29881 gtgccggtgt tggcggaata taatgcggat aatgatccaa acaaaacgag agaatttatt
    29941 gccaaagtat cgggtacatt aggaggtttg gtaactgttg ttacccttgt ggcaatgatt
    30001 gcctcgcctg tggttgccgc tctttttggt acaggttggt ttttagattg gctttacgac
    30061 gggccaaatg ccgaaaaatt cacccaagcc tcttttttgc tcaaaatcac tttcccttat
    30121 ttgtggttta ttacctttgt ggcgttatcc ggtgcggtgt taaataccat cggtaagttt
    30181 ggggtgatgg cattttcccc ggtgttgctg aatgtggcga ttattgcggt agcgttatgg
    30241 ggcagagatt atttcgattc cccggatatt gccttagcgt ggggggtatt tttaggcggc
    30301 ttattgcaat ttttattcca aattccattt atgaaaaaag agggcttact ggttaagcca
    30361 aaatgggcgt ggaaagacga aggtgtcact aaagtccgta aattaatgat tccggcttta
    30421 tttggcgttt ccgttaccca gctaaattta ctgctaaacc aagtgattgc ctcgtttttg
    30481 attaccggct cgattagttg gatgtattat gccgaccgct taattgaatt tccgctcggt
    30541 ttatttggaa ttgcgatttc caccgtcgtg ctgccaagcc tttcccgtat tgcgaaaaac
    30601 aaagaactca ctgaaatcca acgtggagaa cactttcaaa acacaatgga ttggggggtg
    30661 agaatggtgc tgttgctcgg cattccggca atgatcggta tggcagtgct ggctcagccg
    30721 attattatga caatgtttat gcggggtaaa tttggttttg aagatgtgct ggcaacctct
    30781 tatccacttt gggtaatgtg cttggggtta aacagctata tgttgattag cgtgctggca
    30841 aacggttttt atgccaacca aaacaccaaa acaccggtaa aagttgggat tattgccgcc
    30901 ttaagtaata tttgctttgg cttggcgttt gctccgtttt tgggatacat tggtttagcg
    30961 ttagcttctg cgtgttcggc attagtgaat gtgagcttgc tgtatgtgaa tctctcaaaa
    31021 aatggttact ataaagtaag ccataaaacc gtgctttttg tgttgaaact attcatcgcc
    31081 gcttgtgtaa tgggggcgtt ggttgcctat ttcacgcctg agattgaact ttgggcatta
    31141 atgacaatgt ggcagaaagt gtattggctg atttggctga tgatcttagc cggcggtagt
    31201 tattttgcct ctattttggt attaggcatt cgtaaaaaag atttccgctc ggcataaaat
    31261 gaaatcatta aaactgaaaa tcccgccgcc ggcttggttc ttgctttgtg ccgctttaat
    31321 gtggctggta gctcaatatg tgccggtgag cttgccgaat tatcagcata cagccatatt
    31381 ttttagcggt ttagctgtgg cgataattgg cggagcggtt gccgtctcgg cactttgggt
    31441 catacaccaa gcgaaaacga catacagtcc ttttcgacca caacataccg ctaaattggt
    31501 cacttggggc atttaccgca aaagccgaaa cccaatgtat ctttcgttac tgttaggttt
    31561 gattgcgtgg gcattatggt tgggggcaat gttggcgtgg ctggttttac cgctgtttgt
    31621 atggttgata acctatttcc aaatcaagcc ggaagagcaa attttagccc aaaaattcgg
    31681 cacacattat gccaaatatt gcaaccaagt aaggcgttgg ctttaaatgc aagcggtgag
    31741 attttgttgg aaatttgcaa atttttccga aaatctcacc gcttgtgggg ttagtgattg
    31801 aaatcagccg tcattaagtc gtgcgagttg tccatcgcta aattgataaa gcgttcatac
    31861 tgttgcagca cgtcggcaat caactcttct ttggtcatat attgcacatc ataacctttc
    31921 cgcccgtcta agaaataagt aatcggctcg taaatggtat tttgctcaat gttcggtagg
    31981 ttgtcgtcac ctaccaccaa atccgataat tcccgtggca cactttcaat gccgtaaatg
    32041 aaattgcgta aattttcttt caccacttca aactctaatt tcgggttttg ttcgttggtg
    32101 aaattcattt tagcgattaa gccatagctt tcaaattcct cgataagttc taaaaatgcc
    32161 ggtctggcaa ctttaataaa gaagtgacgg acatcttgct tattgcttgg gttgatgatt
    32221 ttctccaagc gttgtttcca atctttaccc gcccaatgtt ggctaccttg cgagaatttt
    32281 ttgttgaagt attggttatc taccattaat cctttccata agccgataca gagaatcagc
    32341 ataatgaagg taaatggtaa ggcgataatc agtgtcattg tttgcagaga agccaagcca
    32401 cccgaataga gtagtgataa cgctaagatg gctaataatg ctccccaaag cacgctttgc
    32461 catttcggtg cattttcttc gccttgtgaa gcaatgctgt tgagaacaaa aatccccgag
    32521 tcggcagagg tgataaagaa aatactgata acaatgacgg caacgaaaga ggcaatagag
    32581 ccaaacggta attgctcaaa aaaggtaaat aacagttgtt cggtattgcc gctgacttca
    32641 gctaaagcac cggcagtttg ttgttcgaac caaatggcag agctgccgaa gctggtcatc
    32701 cataaaatgt taaacagcga tggaacaaat agcaccccta aaataaattc acgaatggtt
    32761 ctgcctttag agatcttggc aataaacaaa ccaacaaatg gcgcccaaga tgcccaccat
    32821 gcccaataaa ggaccgtcca gccgcttagc cagccttgat gttccggttc ataagcgaag
    32881 gtacggaaac tcatctcaag cagcgagcta aaataatagc ctaaattttc ggtgaagctt
    32941 gagaaaagta atagagttgg gccggtgatt atcacaaaaa taagtagcaa tccggctaat
    33001 gtgagattga tttcacttaa gattttaacc cctttgccta cgcccgaaac ggcagataac
    33061 acggcaagag ccattgctac tgttacgata atcgcaagaa cagtaaaatt gctctgttca
    33121 attaaaccta gattgttaaa gccagcactg acctgcatca ccccaaagcc aagtgtagtc
    33181 gttaaaccga aaatcgtgct acaaagggca ataatatcaa ttaaatgccc ccagaagcct
    33241 gaaatacgat gttttagtaa gggataaaat ccggaacgaa tagtgagtgc taacttatat
    33301 ctgaagccaa aataagcaag agctaaggca atcacaccat aaattgccca agcgtgaata
    33361 ccccaatgat agaacgttgt catcatcgct tctttcatac gctccggctc agttaaattt
    33421 tgttggacgg gtttaaggta atgcaaaata ggttctgcta ccccaaaata cattaacccg
    33481 atccccattc cggcggcgaa taacatcgca atccaagagc caaaaccaaa ttcaggctca
    33541 tcggtatctg cccctaattt aatatcaccc aatcggctga aagagagaaa aattagaaac
    33601 aggaaaaaga tagagcctgc aaaaatatag aaccagctaa aatgttggaa gagactattt
    33661 ttgatggtat caagtgaggt ttgtgcttgc tgtggaaaga tgatacagaa aacggcaaca
    33721 aagctgacaa agagtaaact cggtaggaaa atcggtatcc taaaggtact tcctgtgttt
    33781 aattttttga ttaaatccaa tgctatttcc tctatttttg aatggttaga ggcttgaata
    33841 tagcaaaaag atataaaaat aacaaattta agacgtattg gtaataatat tgcaacctaa
    33901 tgggtttggt aaaaaaatcg ggaaatttga ccgcttgcac attcattatt tgcgagtaga
    33961 atatttttca tttataaaaa acgaataaag aatgcacaca caacataacg ctcaaaaaat
    34021 ggttttgccc attttactgc tgatttccct ttctcatttg ttgaatgatt tagttcaagc
    34081 agtaatggtt tcgatctatc caatgctaaa agaaaattac caactgagtt ttgctcaaat
    34141 tggtatgatt tcactcattt atcaaattac cgcatctatt attcagccgg gtattgggct
    34201 ttataccgat aaatacccaa aaccgaattt attgcccgtc agtatgctga ttacgcttct
    34261 ttccgtgatt ttacttgcct tttcgctgaa ttttggcgtg ttactgatag ccgctgcgtt
    34321 aatggggata ggctctgcca tttttcaccc tgaagcctct cgggtggctc gaatggcatc
    34381 gggcggtaaa tttggtacag ctcagtcgct tttccaagta ggtggaaaca gtggatcggc
    34441 gttaggtccg ctcttagttg cattatttat tgtgccgtta gggcaaagtg cggtgatttg
    34501 ggttgttgca tttgtattat tggcattatg atgggtttta tataaagtaa gccgttgggt
    34561 gaaatatgaa gccaaaccgc tcattatttc accaaaccat attcaacaac gcttacacgg
    34621 taaattatta gccagagcat tattaattat cgctttattg atgttagcga aatttattta
    34681 tattgcgagc ctcagtaact atttcacctt ttatttaatg gaaaaattcc aactcaatat
    34741 agcaatggca cagctctctc tatttacctt tttaggtgta gttgctttag gcacttttgt
    34801 cggcgggccg attggcgata agattggacg aaatgcggtg atttggttct ctttcttagg
    34861 tatggcacct tttgctttgt tgttgcttca cgcagatctc ttttggacga ttgtgtgtgc
    34921 gatttttgcc ggttttatta tgtcatcagc gttctccgct atggtggttt atgctcaaga
    34981 agccgtatca ggcagagtag ggctgatttc cggcacaatg ttcggtttaa tgtttggtat
    35041 tggcggctta agtgccgccg tattgggttt tttggcggat gaatatggct tggagagtat
    35101 ttttaaagcc tgtgcctatt tgccactact tggttttgct gcattagggc taccaaaacg
    35161 aaatatttaa cgttttattt ttagcgtttg acaccagctt ttttcatacc caataagaaa
    35221 acccccaccc caatccaaac cagaatgtag cctacagcac gttcggcgga aagtgtctca
    35281 ttaaaaatca gcacaccgca taaaaattgc agggtaggtg aaatatattg cagcataccg
    35341 agtagcgaca ttgaaatgcg tcttgcaccc attgcaaacc atagcaatgg taaggtagtg
    35401 gcaatgccgg agcagagtag cactgccatt tgtaaaggat tgagttcact gaataccaaa
    35461 atgttttggt ggtaacaaaa gcagagatag gtgatggcaa aaggcgacat cagcaaagtt
    35521 tctaacgcca aaccagacaa aggttccatt ggggcaagtt tacgaatcag cccgtaaata
    35581 cagaaactgg cggctagaat caatgcgacc cacggaattt gtccggcagg aatagcaagc
    35641 cataaaatgc cgctaattgc aaacagcaga gccaataatt gggctttatt cagttgctct
    35701 tttagaataa gcctaccgac aaacacgttg aaaatggggt tgataaaata acctagactg
    35761 gcatctaaaa tatgctcatt gacgattgcc cataagtaaa ccagccagtt cacgccaatc
    35821 ataaaagaag aaagcacgaa cagcccgagt acctttggct gtttaaaggc attaataacc
    35881 gctcgccctt gttggaaaat cagcagcaga aacacagcaa atagggcagc ccaaagcacc
    35941 cgttgagcca aaatttgctc ggcaggcatt cccgattggt tgatcggata ccaataaatc
    36001 ggaaaggttc cccaagttaa gtagcacagt agggcatagt gccaaccctg taatttgcct
    36061 gaaatattca ttttgctttc ctaaacgagt gtacaagcgg tcatatttgt cgattttttt
    36121 gcaaattgca aatcacaaaa cggcataaaa aattaggctt tttcaatgcc gaaagaaata
    36181 acctcttcaa ttttttccgc atccattgcc agcattaaca gcctatcaac cccaagggcg
    36241 acacctgagc agtttggaaa acccgctttg agtgcggcaa gaaaacgcac atctagttgc
    36301 tgtggctcca gccccatttt ttcacgttgc agattatctt gctcgaagcg atgaatttgc
    36361 tcatcagcat cgtctaattc gtggaagccg ttacagagtt ccaagccttt gtagtaaaac
    36421 tcaaaacgtt cggcaacacg gtgatcttcc gagctaattt gagctaatgc cgcttgggtg
    36481 gaagggaaat gataaacggc agttggtctt tcttgcccga tattggcttc gacaacctcg
    36541 ctgaataaaa attgtaacaa ggtgtcacgc tgttcatcat catcacattg caagccgtgt
    36601 tttcgggctt tttccacaag ttgtgctttg gtggcagaaa gcggatctaa tccgacatag
    36661 gtttggaaca caaattgata gctgaatgat tctgccggtt cgcagtccaa aatttgttgc
    36721 agtaaatcat ccacttcatt gattaagcga tacatatcaa aatgcgggcg ataccactcc
    36781 agcatcgtaa attcagggtt gtgtcgctta ccggcttcct cgttgcgaaa tactttacac
    36841 agttggaaaa tcggtccact cccagcagca agtagtcgct tcatatgata ttccgggctg
    36901 gtcatcagat ggagtgtttt cgcctcaggg gaaaaaggcg attgaaactg cgtattgaaa
    36961 gtcgaaagat gaacgtctgt caccgaaaat tcgcttaaaa tcggggtttc tacctctaaa
    37021 agccctcgat cggtaaagaa cttgcggatt tccgtaatga tttttgaacg ttggatcagg
    37081 tgaggaatag aggctgttgg ttgccaatca atattatcga gttgtaaatt gttcataaaa
    37141 aatgaccgct tgaaaatgtg aggctatttt agcctaaaaa agtgagttgc cctagttacc
    37201 gatcaagaaa aataaagaga gggttttaac ttttttgcaa caaattaaat tttctcattt
    37261 taaacattag aaattttctt tctatcactg tccaattaag agccattctc aataccttta
    37321 taagctttat tacctttttt gaggtagatc acaaaattac attttttatg ttacaaattt
    37381 gtaaaaatag gttatctcat tgctcaaata gtggcacaat atgattactt tttaatgaat
    37441 taagttattt aactttcgga gtgcattatg caaagtgtta attttgatgt cgcgattata
    37501 ggtgccggtg gtggtggctt gcgtgctgct attgcggcgg ctgaagcaaa tccaaatcta
    37561 aaaattgctt taatttcaaa agtttaccca atgcgtagcc atacggttgc agcagaaggt
    37621 ggttcagcgg cggtaattaa agataccgac tctttcgaca atcactttaa tgataccgtc
    37681 ggtggtggtg actggttatg tgagcaagat attgtggagt attttgtaga gcattcgccg
    37741 attgaaatga cacaattaga acgctggggt tgcccttgga gtcgtcgtga agacggtgaa
    37801 gtgaatgttc gccgttttgg aggaatgaaa attgagcgta cttggtttgc ggcggataaa
    37861 accggtttcc acattttgca tactttgttc caaacttcga ttaaatatcc gaatattgtg
    37921 cgttttgatg agcattttgt gctggatatt ctcaccgaca acggtgaagc tcgtggttgt
    37981 gtggcaatga atatgatgga aggcacactt gttcaaatca atgcaaatgc agttgtgatt
    38041 gcaaccggtg gcggctgccg tacttatcgc tttaacacta acggcggtat tgtaaccggc
    38101 gacggtttat caatggtgta tcgtcacggt gtggctttgc gtgatatgga gttcgtacaa
    38161 tatcacccaa caggtttgcc aaataccggt attttgatga cagaaggttg tcgtggtgaa
    38221 ggtggtattt tagttaataa agatggctac cgttacctgc aagattatgg tttaggaccg
    38281 gaaacaccaa tcggcaaacc ggaaaacaaa tatatggaat taggccctcg tgataaggtt
    38341 tcacaagcat tctggcaaga atggcgtaaa gggaacacct taaaaaccgc aaaaggcgtg
    38401 gacgtagttc acttagattt acgccattta ggtgaaaaat atttacttga gcgtttaccg
    38461 tttatttgtg aattagcgaa agcctatgaa ggggtcgatc cggctaaagc gccgattcct
    38521 gttcgcccgg tggttcacta cacgatgggt ggtattgaag tggatatgaa tgcggaaacc
    38581 tcaattaaag gcttattcgc agtaggtgaa tgtgcttcat caggcttgca cggtgcgaac
    38641 cgtttgggtt ctaactcatt agcagagctt gtggtgttcg gtaaagttgc cggtgaaaat
    38701 gcggctcgcc gtgcgttaga agtgactgct cgcaaccagc cacaaattga tgcacaagca
    38761 aaagatgtgg tggctcgctt gcacgcatta gcgaaccaag agggtaatga atcttggtcg
    38821 gatatccgta accaaatggg tgatgcgatg gaagaaggtt gtggtattta ccgtacccaa
    38881 gaaagtatgg aagcgactgt aaacaaaatc caagagctaa aagagcgtta taaaaacatc
    38941 agcgtaaaag ataaatcaag tgtgtttaac actgatttac tctacaaaat tgaattaggc
    39001 ttcattttag atgtggctca atccattgct tgctctgcgg tagagcgtaa agaatctcgt
    39061 ggggctcacc aacgtttaga ctatgttgag cgtgatgatg tgaactactt aaaacacaca
    39121 caagcgtact acaatgcaga gggtacaccg acgattaaat acagcgatgt gaaaatcact
    39181 aaatcacaac cggctaaacg tgtgtatggt gcagaggctg aagcccaaga aaaagtcaaa
    39241 aaagcagcag aagctcaagc acaacaatag agaaggagta gtaaaatggc aaatttagat
    39301 aaaatgacca tcgaagtgct acgctacaac cctgaatcag atagcgagcc acatttagac
    39361 agatatgaag tgccgtatga cagccaaact tcacttttag atgcattggg cttcattaaa
    39421 gacgaattag aaccggaact ttcttatcgc tggtcttgcc gtatggcgat ttgtggttct
    39481 tgcggaatga tggtaaacgg taagccgaag ttagcttgta aaacattcct gcgtgattac
    39541 agcggtttta tgcgaattga gccgctggcg aacttcccga ttgagcgtga cttagtagtg
    39601 gatttaagcc actttattga tagcatcgag gcaattaaac cttatattat tgataataaa
    39661 gcaccggaag gacagcgtac taaacaaacg ccggcacaat tagagaaata tcgtcaattc
    39721 tcaatgtgta tcaactgcgg tttatgttat gcggcttgcc cacaatttgg tttgaaccct
    39781 gaatttatcg gacctgctgc gattacattg gctcaccgct ataacttgga taaccgtgat
    39841 aatggtcgtg aagagcgtat gaagctactc agcagcaaaa acggtgtttg gagctgtact
    39901 ttcgtgggtt actgctcgga agtctgtccg aaacacgtcg gccctgcttc ggctgtaaac
    39961 caaggtaaac ttgaaagtgc gaaagattat gttatctcaa tgatcaaacc gagataggag
    40021 gaagaaatgt cagcaacaaa acgtagacct tatgtaaggg gaatgaaagc cagctggtgg
    40081 aaaaaactcg acttctataa aatgtatatg gtgcgtgaag ctacctgtct tccaactgtt
    40141 tggttctgca ttgtattatt ttatggagca gttgcgttaa gtaaaggaac atttgctacc
    40201 gattttgtgg atttcttaca aaatccgtta gttgttatcc taaacataat ttcattggca
    40261 gcaatgttat atcatgcggc aacacttttt gtgatgacac ctgccgtcat gtccgtgatg
    40321 gttaaaggta aacacttacc agtctcggtt ggaagaaata ttctttgggc agcaacaggc
    40381 gtaattagcg taattgcatt aatcttggtt tatatttaag aaggggaaga aaatgagtct
    40441 tgatcaaaac ccaaaacgtt caaatgaacc gccggtatgg ttgcttttta gtgctggggg
    40501 aatggtaagt gcattatttt tccctgtggt aatctttatt atcggtttat tactgccatt
    40561 cgggttagta tcaccagata atatcatttc ctttgcccat acttggatcg gcaagctcgc
    40621 tattatggta ttagcgattt tccctatgtg ggcaggaatg caccgtatcc atcatggatt
    40681 acacgacctg aaagttcaca cgccagcagg taatgtgatt ttctacggat tgtctatctt
    40741 atacacaatt ctagtaatca tcgcagtcgt gcagatttaa atgagaaaga ccatcttgta
    40801 taaagcagat ggtcttttca tttttaatat gctaacaagc ggtcgaattt ataagaaaat
    40861 ttgcaaatat tagaaatttt gccgataaaa cgatcgtttt gtattaaata tctgcaaaaa
    40921 agat
//
LOCUS       DANJWC010000003        38682 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.003, whole genome shotgun sequence.
ACCESSION   DANJWC010000003 DANJWC010000000
VERSION     DANJWC010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 38682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38682
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.003"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..467)
                     /locus_tag="PWX02_000084"
     CDS             complement(<1..467)
                     /locus_tag="PWX02_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728146.1"
                     /translation="MDISWYKPRNYLHFDSPITLQQAIKLVANPKHSFYPFLSYTLSV
                     AKIKKDKNEGKLVRLIKNRGISYSSHKDSHIFSYYAYLLNEKYENLLKNKYYILDDSI
                     LAFRKLNKSNIDFARIAFDSIRSAQNCTVLGLDISKFFDHIDHKILKDMWCKV"
     gene            complement(862..5145)
                     /gene="rpoC"
                     /locus_tag="PWX02_000085"
     CDS             complement(862..5145)
                     /gene="rpoC"
                     /locus_tag="PWX02_000085"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDL1728147.1"
                     /translation="MKDLVKFLKAQTKSNEDFDVIKIGLASPDKIRSWSFGEVKKPET
                     INYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRER
                     MGHIELACPVAHIWFLKSLPSRIGLILDMPLRDIERVLYFESYVVTEPGMTDLEKNQL
                     LTEEQFMDAEERWGDEFEAKMGAEGIQALLRDLDLEHQCETLREELQETNSETKRKKI
                     TKRLKTLEAFLQSGNKPEWMVMTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINR
                     NNRLKRLLDLVAPDIIVRNEKRMLQESVDALLDNGRRGRAITGSNKRPLKSLADMIKG
                     KQGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFRPFIYSKLENRGIA
                     STIKAAKKMVEREDPIVWDILAEVIREHPILLNRAPTLHRLGIQAFEPLLIEGKAIQL
                     HPLVCAAFNADFDGDQMAVHVPLTLEAQLEARALMMSTNNVLSPASGDPIIVPSQDVV
                     LGLYYMTREKVNGKGEGMYFLDPREAEKAYRTGQAELHSRVKVRVTEYEKNEAGELEA
                     KTSLVDTTIGRSILWMIAPKGMPFSLFNQTLGKKAISKLINESYRRLGLKESVVLADQ
                     IMYTGFAYAARSGASVGIDDMVIPEQKYAIIEAAEAEVAEIQEQFNGGLVTAGERYNK
                     VIDIWAAANERVAKAMMENLSTEEVINREGNPEKQASFNSIFMMADSGARGSAAQIRQ
                     LAGMRGLMARPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLT
                     RRLVDVAQDLVIIEDDCGTHEGIVMTPLIEGGDVKEALRDRVLGRVVAEDVLKPGTDE
                     VLIPRNTLLDEKWCDVVDRESVDVIKVRSVVTCDTDFGVCAKCYGRDLARGHLINQGE
                     AVGVIAAQSIGEPGTQLTMRTFHIGGAASAAAKESSIQVKNAGTIKLTNAKFVTNKEG
                     KIVLTSRNTELTVIDTFGRTKENYKVPYGAVLSKHDGAEVAVGEVVANWDPHTMPVIS
                     EVSGRIQFSDIVDGLTVTRQTDELTGLSSIVVQDVGERATAGKDLRPALRLVDSNGKD
                     ILIPGTDVAAQYFLPGKAIVTLDDGAEIEVGEALARIPQESVGTKDITGGLPRVADLF
                     EARKPKEPAILAEISGIVSFGKETKGKRRLVITPAEGEAFEEMIPKWRQLNVFEGEMV
                     QRGDVISDGAETPHDILRLRGVHAVTDYIVNEVQEVYRLQGVKINDKHIEVIVRQMLR
                     KAVITNAYDSEFLEGEQVEVARVKIANRKRAEEGKPLVEFERELLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKQDELRGLKENVIVGRLIPAGTGFAYHQARAKKRAQLEA
                     GIAVVPEFEAPAAKANSFVTDADIEAEFEFVADDATQSLAALLNAGEEE"
     gene            complement(5408..9433)
                     /gene="rpoB"
                     /locus_tag="PWX02_000086"
     CDS             complement(5408..9433)
                     /gene="rpoB"
                     /locus_tag="PWX02_000086"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL1728148.1"
                     /translation="MAYSYSEKKRIRKSFGKRPQVLNVPYLLTIQLDSFEKFIEKDPE
                     GQQGLEAAFRSVFPIVSNNGATELQYVSYELGEPVFDVRECQIRGTTYAAPLRVKLRL
                     VSYDREAAAGTVKDIKEQNVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFARIDRRRKLPATIILRALGYTTE
                     EILNLFFEKIKFEIADNKLLMTLVPERLRGETASFDIAANGKVYVEMGRRITARHIRA
                     LEKDNVTQIEVPVEYIVGKVAAQDYVDLSSGEVICPANMEISMEMLANLSQAGYNQIE
                     VLFTNDLDHGPYISETLRVDPTYDRLSALVEIYRMMRPGEPPTKEAAEALFDNMFFSA
                     ERYDLSAVGRMKFNRSLEIPEGVGSGILSNDDIIGVMKKLIEIRNGRGEVDDIDHLGN
                     RRIRSVGEMAENQFRIGLVRVERAVRERLSLGDLDGVTPQDLINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEVTHKRRISALGPGGLTRERAGFEVRDVHPTHYGRLCPIETPEG
                     PNIGLINSLSVYARTNNYGFLETPFRKVVNGQVTEEIEYLSAIEESNNIIAQANSNLD
                     ENFRFTDTYVTCRQQGESGLFKPEEVQYMDISTQQVVSVAAALIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMEKPIALDSGVAIVAKRGGTVQYVDASRIVVKVNEDE
                     TIPGEAGIDIYNLIKYTRSNQNTCINQVPCVNLGEPVARGEILADGPSTDLGELALGQ
                     NIRVAFMPWNGYNFEDSMLVSERVVQEDRFTTIHIQELSCVARDTKLGAEEITADIPN
                     VGESALSKLDESGIVYIGAEVKGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGTSGTVIDVQVFTRDGVEKDKRAKEIEEMQLCEAKKDLAEELEILEAGLF
                     TRVRNLLIEGGVKESALDGVAREKWLEQTLEDEAKQNQLEQLAEQHEELRKEFERKLE
                     IKRNKIIQGDDLAPGVLKVVKVYLAVKRQIQPGDKMAGRHGNKGVISKINPVEDMPYD
                     ENGQPVEIVLNPLGVPSRMNIGQILETHLGLAARGIGDQIDKMIKQQQEVAKLREYMQ
                     KAYDLGHGSQVVDLSTFSDEEVMRLAQNLRKGLPLATPVFDGAHESEIKGLLELGGLP
                     TSGQITLYDGRTGEKFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGKA
                     QFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGTHYMEPGIPE
                     SFNVITKEIRALAIDMELDEA"
     gene            complement(9783..10682)
                     /locus_tag="PWX02_000087"
     CDS             complement(9783..10682)
                     /locus_tag="PWX02_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728149.1"
                     /translation="MVMAEENNTWVDKKHETVSTTLDRWANNINSWLGDVDPDKPGTA
                     SLRVMLDSRWDKHDKFTYKPRVRGKIKLPILKKHLNVVFGDDELDNELTDKNHAGKLY
                     KNVNPNKRYNSREARDSNSSIGLRWSDGIKALGIETDLDAGIRSGSDIFGRLRISKDW
                     KITDQFSTRLEQIYRYGSESKHYLRTNFENKYTETDTTFIMNHTNFEYRHDVDEERSW
                     GNSLYRQHNFKPLTHISYGISMGGRLDKELSKFNYWGPFVSYRQPIWRNWLFIQPEIS
                     FYNDKDKDRKHFIQTFVRLEAIF"
     gene            complement(10798..11073)
                     /gene="tusB"
                     /locus_tag="PWX02_000088"
     CDS             complement(10798..11073)
                     /gene="tusB"
                     /locus_tag="PWX02_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HDL1728150.1"
                     /translation="MLYTLSKANYDEETLNFFFSHLSENDAVVLWQDGVLQAVKSPQF
                     FAKLPRCYLLAQDVQARGLQEKLTAFQCISLSELVKLTAVYFPQVAL"
     gene            complement(11083..11454)
                     /gene="tusC"
                     /locus_tag="PWX02_000089"
     CDS             complement(11083..11454)
                     /gene="tusC"
                     /locus_tag="PWX02_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HDL1728151.1"
                     /translation="MKKYKLAFLFTQPPFGSAVSREGLDALLAASAFCDEQEIAILFI
                     NDGVFNLIADQQPEKLLQKDHISTFKLLELYDLTECFICQESVSLRGLDNADWLLRML
                     QFKTHAEMIALLNQAEKVMTF"
     gene            complement(11456..11833)
                     /gene="tusD"
                     /locus_tag="PWX02_000090"
     CDS             complement(11456..11833)
                     /gene="tusD"
                     /locus_tag="PWX02_000090"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HDL1728152.1"
                     /translation="MDYVLAIKSPVYGSQGAFLAYQVAEELLKANHTICQIFFFQEGV
                     SNANALVNPASDEVNLVEMWKKLAKLHRLPLHLCIAAAQRRGVVDEQISANATHNLAE
                     GFTLAGLGEFSQAVLKADRLLTF"
     gene            complement(11833..12489)
                     /locus_tag="PWX02_000091"
     CDS             complement(11833..12489)
                     /locus_tag="PWX02_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL1728153.1"
                     /translation="MTKKFTPFSDEDHAILASYFPMVDGIAALIGEHCEIVLHSLEFL
                     EHSAIYIANGHNTNRKIGSPITDRALKSLHHMATDSVSKPYFTKAKGGVLMKSITIAI
                     RNGKQHVIGLLCININLEVPTSQFLNSFIAPVDTASEVTFASSVEDLVSQTIQTTIDD
                     VMADRQISNNNKNRQIVISLFEKGIFDIKDSINQVAEKLDISRHTVYLYIRQIKQDND
                     "
     gene            complement(12586..13311)
                     /gene="fkpA"
                     /locus_tag="PWX02_000092"
     CDS             complement(12586..13311)
                     /gene="fkpA"
                     /locus_tag="PWX02_000092"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL1728154.1"
                     /translation="MLKTKLSLFTLIVGTLFSTNVMAEEKADPKFVDDSSYAVGVLMG
                     KNINGVIESQKDIFTYNQDKILAGVQDTLKKTGKLTDEDLQKQLKSLDAYLSAKEAKI
                     NEEKTKATVAAGDKFRADYKKQSGVKETASGLLYKIEKAGEGNSPKAEDTVKVHYKGT
                     LTDGTVFDSSYDRGEPIEFQLNQLIPGWIEAIPMLKKGGKMEIVVPPALAYGDRAAGK
                     IPANSTLKFEIELLDFKAADAKK"
     gene            13399..13620
                     /locus_tag="PWX02_000093"
     CDS             13399..13620
                     /locus_tag="PWX02_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HDL1728155.1"
                     /translation="MDNNYTLSAKITELETKVAFQELIIEELNQALIEQQFAIDKLQT
                     QIRHIAEKLKGAQLSQVASQAEETPPPHY"
     gene            13678..14370
                     /gene="mtnN"
                     /locus_tag="PWX02_000094"
     CDS             13678..14370
                     /gene="mtnN"
                     /locus_tag="PWX02_000094"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDL1728156.1"
                     /translation="MKIGIIGAMAQEIEILRQVMVEPKVTELAGCKIFEGKINNTRVA
                     LLQSGIGKVAAAIGTTLLIQLTQPDMIINTGSAGGLDPDLNVGDILISTEVRYHDVDV
                     TAFGYEIGQLPANPPTFSPNEQLVELAQKEAKKAGYNVVAGLICSGDAFINGNDKINQ
                     IRQNFPNVVAVEMEAAAIAQVCHGFNLPFVIVRAVSDVADKESHLSFEEFLPLAAEKS
                     STIVLAMLNNID"
     gene            14525..15862
                     /gene="rimO"
                     /locus_tag="PWX02_000095"
     CDS             14525..15862
                     /gene="rimO"
                     /locus_tag="PWX02_000095"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDL1728157.1"
                     /translation="MSASPNIGFISLGCPKNLVDSERILTELRADGYNIIPSYENADL
                     VIVNTCGFIDSAVQESLESIGEALEANGKVIVTGCLGAKENQIREVHPKVLEITGPHS
                     YEAVMNHVHKYVPKPEFNPYTSLVPKQGIKLTPKHYAYLKISEGCDHRCTFCIIPSMR
                     GDLASRSIVQVLDEAKRLVDAGVKELLIVSQDTSAYSLDRKKEEETKTVFWNGMPIKS
                     NLITLCEQLGSMGVWVRLHYVYPYPHVDDLIPLMAAGKILPYLDIPLQHASPKILKAM
                     KRPGSIERTLERIKKWREICPELTLRSTFIVGFPGETEEDFQLLLDFLKEAQLDRVGC
                     FKFSPVEGAVATDMPDQVPEEVKEERFHRFMQLQQEISAARLQAKIGKTVQVIIDDID
                     EEGIIGRSMADAPEIDGVVYVDNPSNQLVTVGQIISVTITHADEYDLWGILNN"
     gene            15872..17881
                     /locus_tag="PWX02_000096"
     CDS             15872..17881
                     /locus_tag="PWX02_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL1728158.1"
                     /translation="MSAENMPSVIRFEQAVAKKDYESACTELLSILSKLDSNFGGISN
                     IELNMPEQIENLENDKAIYFCTRMAVAITRLFEDPALEISEHGAMRFLTLQRWIALIF
                     ASSPYVNADHILRTYNRNKESANPNTVDLDATLQALIKFCILYLPESNILLNLDAAWN
                     ASSDLTASLCFALQSPRFIGTSSAFAKRAAILQWFPEKLAQIENLNKLPSAISHDVYM
                     HCSYDIEANKHNVKRSLNAVIRRHLLSVGWEDRKIEQLGTRNNKPVMVVLLEHFHSSH
                     SIYRTHSTSMVAAREHFHLIGLGSDAVDEMGQQVFDEFHLLPQDGSLFDRLSFLKDIC
                     DKNNPAVFYMPSIGMDLTTIFASNTRLAPIQAVALGHPATTHSDFIEYVIVEDDYVGS
                     ESCFSEQLLRLPKDALPYVPSALAPQNVVYNLRENPEVIHIGIASTTMKLNPYFLEAL
                     KAIRDRAKVKTHFHFALGQSSGITHPYVERFIKSYLGNDATAHPHSPYDEYLNILHNC
                     DMMLNPFPFGNTNGIIDMVTLGLVGVCKTGPEVHEHIDEGLFKRLGLPNWLITQTAEE
                     YVTQAIRLAENHEERLAIRRDIIENNKLQTLFSGDPRPMGQIFLAKVQAWLADKNPKN
                     AEVEVKTKKVRKAATASQSAKKQTTSKTQTAKAEKDNAAKTETKS"
     gene            complement(17960..19217)
                     /locus_tag="PWX02_000097"
                     /pseudo
     CDS             complement(17960..19217)
                     /locus_tag="PWX02_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005760733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
     gene            complement(19230..20018)
                     /locus_tag="PWX02_000098"
     CDS             complement(19230..20018)
                     /locus_tag="PWX02_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HDL1728159.1"
                     /translation="MAALSEIFVPLHRIIPFSNVEGQGNRTSIFLQGCKLNCLYCHNP
                     ETIPRYSKEAKQVSLQYLYDQVMEAVPFIRGVTISGGEPTIHHKKLVPLFEALREQGL
                     TCYLDSSGFFDFEATEPLINVTDKFLFDLKGDGAGLQTLCFDRKNQAGKVPEQALPNV
                     NHIKPENLARNLQNLAKLLPLDKVEEVRLVYLNEFYNAHHLLEKVADLLKPYPDVLLK
                     IIRVHTKGARDETGLARFVPTIEQTDELAAFAKRHGIQKVITIY"
     gene            complement(20021..21562)
                     /locus_tag="PWX02_000099"
     CDS             complement(20021..21562)
                     /locus_tag="PWX02_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HDL1728160.1"
                     /translation="MQASLQDILEVVKAKNLTYQQKLMSLGNIAERLFNPIELLGYTE
                     EEWEHIENQMICDLNEGYAIYRPRYILPDYNVYMQKGCQFLDLPPPTNLDEALDGLLI
                     LYSHVPSITTFPVYIGRLDQLLEPFITDEEQDYIKIKRFLNHIDKTISDSFCHANIGP
                     NDTKAGRLILKAVIELENTTPNMTIRYDKTKTSREFAELAAKACLLVSKPSFANDAYY
                     IQDLGEEYGIASCYNALPECGGAYTLTRLRLGTIGRACASVEQMVNEMLPKVAKLALS
                     TMDKRHKFLVEESNFFESSFLVQEGFIKRTNFTSMIAIVGLADAVNHLLKQEGLNETF
                     GQSKRGDEIATLIMDKLQAVNDAHKGLYVERTNNQYLLHAQVGANNHAEDKMNTPAHR
                     IRVGEEPTLLAHLKQSAPFHKYFPSGTGDLFAFDQTYTDHLDAVVDIIDGSFAQGYRY
                     ITTYLKNTDLIRVTGYLVKKSEVERYRKGEAVLRDTTWYGSGTDDCAKVFDRQLRDEE
                     NVNAG"
     gene            21788..22618
                     /locus_tag="PWX02_000100"
     CDS             21788..22618
                     /locus_tag="PWX02_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL1728161.1"
                     /translation="MVQKPLNHYLEMQEHFLYVPYLHHHRRIRVLLPKDYHNEGWERY
                     PVLYMHDGQNVFYSKESYSGHSWKIIPTIKSHQELPKMIIVGIDNAGANRLNEYGPWK
                     TTTGSTPETRNAGGLGEEYAKWVVETVKPFIDIHYRTKPQQEHTLLAGSSMGGIITAY
                     MGSRYPHIFGHLGVFSLASWFSEPDFLRFTHQYPLQPNTKVFIQVGTNEGDEIDSHFI
                     SNTNQTYIDCSLNYYQALIRIGVPLDNIRLRIMANEIHHEMHWADHFVEFLHFSLLRK
                     "
     gene            complement(22725..23093)
                     /gene="rplL"
                     /locus_tag="PWX02_000101"
     CDS             complement(22725..23093)
                     /gene="rplL"
                     /locus_tag="PWX02_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDL1728162.1"
                     /translation="MSLTNEQIIEAIASKSVSEIVELITAMEEKFGVSAAAAVAAAPA
                     AGAAAAEEKTEFDVVLAEAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGISK
                     AEAEALKKELEEAGAKVEIK"
     gene            complement(23152..23643)
                     /gene="rplJ"
                     /locus_tag="PWX02_000102"
     CDS             complement(23152..23643)
                     /gene="rplJ"
                     /locus_tag="PWX02_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDL1728163.1"
                     /translation="MALNLQDKQAIVAEVNEAAKGALSAVVADSRGVTVEKMTELRKA
                     AREAGVTMQVVRNTLLRRAVEGTEFECLTDTFTGPTLIAFSNEHPGAAARLFTEFAKT
                     NKEFELKGAAFEGKKQDVAFLATLPTYEEAIARLMGTMKEAAAGKLVRTLAALRDQME
                     AAA"
     gene            complement(23924..24139)
                     /locus_tag="PWX02_000103"
     CDS             complement(23924..24139)
                     /locus_tag="PWX02_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL1728164.1"
                     /translation="MDINDIPQDNSKIFNGERKVIYARNPQPEIRQMISSILACYAWD
                     ENNPFVAKSDARLKALAEVVGKAEIGD"
     gene            complement(24188..25330)
                     /locus_tag="PWX02_000104"
     CDS             complement(24188..25330)
                     /locus_tag="PWX02_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HDL1728165.1"
                     /translation="MLSNVYITNTSAFLPNAPVSNDAMEAVLGMVGETPSRVRKMILR
                     SNGIQYRYYAIDPITRQATHSSTELAVQAVKKLGNSATSATCLAVGTSYPDQIMPGQG
                     VMVHGLLDAEPMEVLTTAGVCVAGMAAMKHAYNAVRIGEHRAAIATASETASAIMRSE
                     HFRAEIDHKKLDDARPEIGFEKDFLRWMLSDGAGAVLLANQPNEKGLSLKIHWIDLVS
                     YANEMPVCMYAGAEFIDGNYTGWKAMSEAQRAECSAMAVKQDVKLLNENIVKYTVEKA
                     LERIIPKYKLNAEEIDYFLPHYSSGSFRDKLLEGLQRINFLIPQEKWFTNLTTKGNTG
                     SASIYIMLDEFIRTHDLKQGQKILCYVPESGRFSSCFMLLEAVVNS"
     gene            complement(25353..26258)
                     /locus_tag="PWX02_000105"
     CDS             complement(25353..26258)
                     /locus_tag="PWX02_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dialkylresorcinol condensing enzyme"
                     /protein_id="HDL1728166.1"
                     /translation="MKKILLVYYSQTGQLTRLAENFVQPLEQAGVFVEKLAIKPQQEY
                     PFPWRFMRFFNTFPETVHLTPPPIEPLPFQHEIYDLVIIAYSVWFLSPSQPITAFLQS
                     EQAKKVLKDTPVITLIGCRNMWLMAQEKVKKLLAESEAKLIGNVVKVDQSNDWASFIT
                     TPLWMLTGKKKIGKLPSAGIAESEMEDVKRFGAKLLHIFSQNQPLDSTLFRQMQAVKI
                     DEKLMMSEKVGARSFHLWGKLLIKCGQISPHLRQAVLYFYILFLVTLILTVVPISTVI
                     KRLLRPLLKEKLEKQKRYFAEPSGE"
     gene            complement(26272..27417)
                     /locus_tag="PWX02_000106"
     CDS             complement(26272..27417)
                     /locus_tag="PWX02_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HDL1728167.1"
                     /translation="MKRVVITGIGAVTAFGKEWAEIHGYQPPKHWNRKQLRSLGRVSQ
                     FVVEASERALAQAGLLDENGVIFDWLKDGRMGVACGSSTGSTNDIKDAAELLMTGKSD
                     GFNANTYVRMMPHTTAANIGIFFGLTGRIIPTSSACSSGSQGIGYAYEAIKYGMIPMM
                     LAGGGEEFCASEVYVFDSLYAASRRNDNPKGTPRPYDKDRDGLVIGEGAGMFVLEELE
                     HALARGANIIAEVVGYGANSDGSHVTRPQATTMQRCMELALKDAGIQPNQVGYVNGHG
                     TATEQGDIAETQATTAVFGKIPLSSQKSYLGHTLGACGALESWFSIEMMRDGWFAPTL
                     NLENVDERCGDLDYLQGDGREIHTDYVMNNNFAFGGMNTSLIFKHWK"
     gene            complement(27480..28208)
                     /gene="fabG"
                     /locus_tag="PWX02_000107"
     CDS             complement(27480..28208)
                     /gene="fabG"
                     /locus_tag="PWX02_000107"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012771807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDL1728168.1"
                     /translation="MNNTVLITGSSRGIGKAIALTLAQQGFDIVVHCRSRVEEAEAVA
                     QAVRNLGKNARVLQFDVADREKVRSILEADVEQHGAYYGVVLNAGLTRDNAFPALTDE
                     DWDSVLRTNLDGFYNVLHPIMMPMIRRRKAGRIVCITSVSGLIGNRGQVNYSASKAGI
                     IGAAKALAIELAKRKITVNCVAPGLIDTDILDENVPIEEILKMIPAGRMGDPDEVAHA
                     VAFLMDEKAAYITRQVIAVNGGLC"
     gene            complement(28222..29655)
                     /locus_tag="PWX02_000108"
     CDS             complement(28222..29655)
                     /locus_tag="PWX02_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HDL1728169.1"
                     /translation="MQEAYDIQHLCTRKEDKSFDRKSARKEPKEILRHLIGFANADGG
                     LLVIGIEDNGEITGFNYQKAHQPEEFIHALHNLQKMPIPVTYQQIEVVNSRGLADFVL
                     LFEVTASSGRAITSHDDRVFLRSKDQTLELNFEQRLQLEYEKGQRAFEEQPIARADFT
                     DLDMDLLEQYRQKLHTDRSIEEILKARDLINKEGQITNACILLFGNNPTRYFPNARLR
                     FLRYEGKAPLTGRTFNVVKEFMLEEPIPKLLESAKAVLKSQLREFQRLDENGQFTKID
                     EYPEFAWLEGIVNAIAHRSYSNQGDCIRISMFDDRLEIFSPGGLPRPVTLENMYYTRF
                     SRNPKIARVLYEFGWVKELNEGVNRMAEEMNAYQLNTPSYFEPNENAVRLILENNIEH
                     RTLRALERIEEIFTQDIMATLNKNEVTILQYLYENEMITVKIASEILDRSKELSRRIL
                     KSLEQKNILTWHGLSPKDPTQYYTLKK"
     gene            complement(29745..30185)
                     /locus_tag="PWX02_000109"
     CDS             complement(29745..30185)
                     /locus_tag="PWX02_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005557532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog family protein"
                     /protein_id="HDL1728170.1"
                     /translation="MHKFTFPIEQIAPLVPHSGNMILLDRITAFGEDFLTAETNIRPD
                     NPLIKHGKLATYASIEIMAQGVAAWAGCIATQAGEPVRLGYLLGTRKLHLHRQEIPIG
                     SQLQIQIKMSIQDATGFGVFDCQLIDLADNQVLLEGALNVFSPK"
     gene            complement(30178..31401)
                     /locus_tag="PWX02_000110"
     CDS             complement(30178..31401)
                     /locus_tag="PWX02_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005544283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HDL1728171.1"
                     /translation="MMTLYLSEPAITSPIGYGIVEHLNVLINAKVSPLDTTSQPYLRH
                     NISNSSPKFLGQVSARLRDFPVELSIEHRSRNNQLLWHALAEIEPKITACIEQFGRER
                     IAVIIGTSTTGADENIPVFKAGVQGDYATEQFNQQQILFSAPSDFVAEVYGLTGLSYG
                     ISTACTSGARALISAARLLKAGLCDAVICGGVDTLSPLTISGFSSLEVLSNQQTNPFS
                     ANRNGINIGEGAAVFVMSREPLDEHRIQLLGYGSSSDAYHMSSPHPEGEGAISAFQNA
                     LHNADLDAADIGWINLHGTGTLHNDQMESLAVAKIFGNQTACTTTKPYTGHTLGAAGA
                     VEAAILWGMLCRQHNPTGKLPAQLWDKQADENLPNIAITYEQSRWAAGRRIGASSSFA
                     FGGNNSVLILGESDA"
     gene            complement(31488..31907)
                     /locus_tag="PWX02_000111"
     CDS             complement(31488..31907)
                     /locus_tag="PWX02_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728172.1"
                     /translation="MLRLLSFCFIFLLSACTVLPQIKQLPPQVESVKSFKVENNGQQS
                     LLLIQFYENKWRWIQTDPLGAPIARVWLTKKGWEPDGFVMPNKQAQLLFTALVNALNP
                     HSPPFYLDKGWKIEREKSQFTIWLPDSSKWKVEILGE"
     gene            complement(31969..32658)
                     /gene="rplA"
                     /locus_tag="PWX02_000112"
     CDS             complement(31969..32658)
                     /gene="rplA"
                     /locus_tag="PWX02_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDL1728173.1"
                     /translation="MAKLTKKMKAIKAGVDSTKAYDINEAVEVLKKFATAKFVESVDV
                     AVNLGIDSRKSDQNVRGATVLPHGTGRTARVAVFTQGANAEAAKAAGADLVGMEDLAE
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKSGQ
                     IRYRNDKNGIIHTTIGKADFSAEQLKDNLNALLAALTKAKPTTAKGIFIKKVSISTTM
                     GAGVAVDQASL"
     gene            complement(32663..33091)
                     /gene="rplK"
                     /locus_tag="PWX02_000113"
     CDS             complement(32663..33091)
                     /gene="rplK"
                     /locus_tag="PWX02_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDL1728174.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     RTESLEKGLPIPVVITVYADKSFTFVTKTPPAAVLLKKAVGIKSGSGKPNKDKVGTVT
                     QEQIRQIAETKAADMTGATIETKMKSIAGTARSMGLIVEE"
     gene            complement(33324..33887)
                     /gene="nusG"
                     /locus_tag="PWX02_000114"
     CDS             complement(33324..33887)
                     /gene="nusG"
                     /locus_tag="PWX02_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDL1728175.1"
                     /translation="MSEVEVKENGSKRWYVLQAFSGFENRVAVTLREYIKLHKMEEQF
                     GEVLVPTEEVVENVGGKRRKTERKFFPGYVLVEMEMNDDTWHLVKSVPRVMGFIGGTA
                     DRPAPITKREADRILNRVQETAEKPRHRKEFQPGESILVTEGPFADFSGTVEEVDYEK
                     GRLKVSVSIFGRATPVELEFSQVEKQS"
     gene            complement(33889..34302)
                     /gene="secE"
                     /locus_tag="PWX02_000115"
     CDS             complement(33889..34302)
                     /gene="secE"
                     /locus_tag="PWX02_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDL1728176.1"
                     /translation="MATEIKTKTPEQVEQKGGKSKGANTALWLVAIVFLAVAAVGNAY
                     FSSHFSLIVRVLLLVVLLVGAVALAAITNQGQKAIHFIKESRTELRKIIWPTRPEATQ
                     TTLIVLAMCVVVSLVLWGIDSIIVALITFVTSLRF"
     gene            complement(34415..35437)
                     /locus_tag="PWX02_000116"
     CDS             complement(34415..35437)
                     /locus_tag="PWX02_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1728177.1"
                     /translation="MANGTSRNRRSSGRVTLADVAKVVGVGQMTVSRALRTPEMVSEP
                     LRKRIEEAIIELGYIPHFSARQLASAESHNLVIVTTSISSTENTLILSALRKSVADSA
                     LQMVILIADQESWFQELINHAPQAIVFLNLDCPPNVREWIKNSNIPTVEIGAIKKEPI
                     HLSVGIDSHEAMEKMIHFLVKKGYREIGLLSAKQDLSIFKQYLESWHTTLFAHHISPL
                     KIHHVVEDISFSAGATLLTEALLNWEAIDALVFLSDELACGALYEALRRHMSIPKDLA
                     IAGLGGLEVGSISYPKLTTIAIPYEQMGKIAGKQLLTLLSGKTLSAEEMIVKLPTKLV
                     IRDSTA"
     gene            35668..35937
                     /locus_tag="PWX02_000117"
     CDS             35668..35937
                     /locus_tag="PWX02_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1728178.1"
                     /translation="MKIMAVCGSGLGSSFMMEMNIKKVLAKLGVEAEVSHTDLSSVVA
                     DDADLFVMAKDIAASSNVPKEKLIVVKNIVGLAEFEEKLTAYFNQ"
     gene            35950..37305
                     /locus_tag="PWX02_000118"
     CDS             35950..37305
                     /locus_tag="PWX02_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL1728179.1"
                     /translation="MESLLFFILDILKVPSVLVGLIALVGLVAQKKSVSDIVKGTIKT
                     ILGFLVLSGGATVLLSSLTPLGGMFEHAFNVQGIIPNNEAIVSMAIEKYGTATALIMA
                     FGMVANILIARFTRLKFIFLTGHHTFYMACMIGVILTVAGFEGIQLVFVGALTLGLIM
                     AFFPTIAHFYMKKVTGSNDVALGHFGTLGYVLAGAIGQLVGKGSKSTEEMDLPKNLGF
                     LRDSSISISLTMMAIYYILAIASGSEYVSTLSGGQHYLVYATIQAITFAAGVYVILQG
                     VRLILAEIVPAFTGFSEKLVPDAKPALDCPIVFPYAPNAVLVGFLASFLGGILSLAIL
                     GQLNWVLILPGVVPHFFCGATAGVFGNATGGRRGAIIGAFIHGILITFLPVFLLPVLG
                     SLGFANTTFSDSDFGGIGIVLGYMAQYFDKNIITLIIIGLFTLLVAYNYLAKKPAEPK
                     A"
     gene            complement(37400..38545)
                     /gene="dprA"
                     /locus_tag="PWX02_000119"
     CDS             complement(37400..38545)
                     /gene="dprA"
                     /locus_tag="PWX02_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDL1728180.1"
                     /translation="MAYQYDLLRLLQVQIGPQRIGRLLAELDFAEFCAYDKTQLRQIG
                     WNEKQIQRWFNPDKNWIEQALTWAEQPNQQILSLFDKAYPFLLRQISTAPPILFVKGN
                     VESLSLPQIAIVGSRDYSPYGEYWAGQFAADLVKHNMAVTSGLAIGIDGFAHKKVVEY
                     QGITIAVLGSGLNRIYPARHQKLAEQIVESGGALVSEFSPNQPPIAENFPRRNRIISG
                     LSLGTLVIEATINSGSLITARYALEQGREVFALPNAVQNPYAQGCHKLIKEGALLVEG
                     VEDILEAVASQYQPQQIPLFDEPKPIVSTSLQAVKSSEKFAKNPPNLTACQQQIYQHI
                     SLEPIAIDDLARATELTIETLLVELLGLELAGVIKQVSGGYVMDNGY"
BASE COUNT        10834 a         8651 c         7658 g        11539 t
ORIGIN      
        1 aaaactttac accacatatc tttcagaatt ttatggtcta tatggtcaaa gaacttagat
       61 atatctagcc ctaaaaccgt gcaattttga gcgcttcgaa tactatcaaa agcgattctg
      121 gcaaaatcaa tattgctttt atttagtttt ctaaatgcta atatagaatc atctaaaata
      181 tagtacttat ttttcaaaag attttcatac ttttcattta ataaatatgc ataataacta
      241 aaaatatggc tatctttatg agatgaataa gatattcctc tattttttat taaacgtact
      301 aatttacctt catttttatc cttttttatt ttagccacag atagtgtata agataagaat
      361 gggtagaaac tatgttttgg attagcgact aatttaattg cttgttgaag agttatgggt
      421 gaatcgaaat gtaagtaatt acgaggctta taccaagata tatccataat agaaaattta
      481 atgaaagcaa aactaaagac taaggattgc ggttgtccct aactatttga atagttaaca
      541 gtctttatat agcctaggta ccttaggata cccatcgaat aaaccgctat ctgctaaata
      601 tatattataa taatcaatca actattataa ctcacaagga atctaatatg acaataatca
      661 ttatcaaatt ctttattagt ttatgttcag tagctgaaac gctttatgaa atgttatcaa
      721 ctattgacat actgttgatt accttatcca caaattgaat aagcccttac attctatata
      781 tttttccctt atattcaagt agaaaacctc ctagaaatta tattctagga gattttttaa
      841 tttatcatac cctacgagct attactcttc ctcacccgca tttaacaacg ctgctaaact
      901 ttgggttgca tcgtctgcaa caaattcaaa ttcagcttcg atgtctgcat cggtaacgaa
      961 tgaattggct ttagctgccg gtgcttcaaa ttcagggaca actgcaattc ctgcttccag
     1021 ttgggcacgt tttttagctc gtgcttggtg gtaagcaaaa ccggtacctg ccgggattaa
     1081 gcgaccaacg attacgttct cttttaagcc acgcaattca tcttgtttac ctgccactgc
     1141 ggcttcggta agcactcgag tggtttcttg gaacgaggct gcagaaataa agctttctgt
     1201 tgcaagagat gctttggtaa taccaagcaa ttcacgttca aattctacca acggtttgcc
     1261 ttcttcagca cgtttgcggt tggcaatttt cacacgagcc acttccactt gttcgccctc
     1321 taagaattca gagtcgtaag cgttggtgat cacagcttta cgcaacattt ggcgaacaat
     1381 cacttcgatg tgcttgtcgt taatttttac cccttgtaag cggtaaactt cttgcacttc
     1441 gtttacgatg tagtcggtaa cggcgtgaac gccacgtaag cgtaagatgt cgtgcggagt
     1501 ttctgcaccg tctgatatca catcaccacg ttgtaccatc tcgccttcaa atacgttgag
     1561 ctgacgccat tttggaatca tctcttcaaa cgcttcgcct tccgccggag taattaccaa
     1621 gcggcgttta cctttggttt ctttaccgaa cgacacaata cctgaaattt ccgccaagat
     1681 tgccggctct ttcggtttac gggcttcgaa taagtctgct acgcgtggaa gaccaccggt
     1741 aatatctttc gtccccacag attcttgtgg aatacgggct aaggcttcac cgacttcaat
     1801 ttctgcacca tcgtctaagg ttacgattgc tttacccggt aagaagtatt gtgctgcaac
     1861 gtctgtaccc ggaattaaga tgtctttgcc gttgctatca actaagcgta atgccggacg
     1921 taaatcttta cctgcggttg cacgttcacc cacatcttgc accacgatag atgataaacc
     1981 ggttaattca tcggtttggc gagtaacggt taagccatct acgatgtcgc taaattggat
     2041 acgacctgat acctctgaga ttaccggcat tgtgtgcgga tcccagttcg caaccacttc
     2101 acccacagcc acttctgcac cgtcgtgttt agaaagcacc gcaccataag gcactttata
     2161 gttttctttg gtacgaccga aggtgtcgat gacagttaat tcagtattac gagaagttaa
     2221 tacgattttg ccttctttgt tggttacaaa tttagcgttg gttaatttaa tcgtacctgc
     2281 gtttttcact tggatgctag attcttttgc tgccgcagaa gccgcaccac cgatatggaa
     2341 cgtacgcatc gttaactgtg tacccggctc accgatagat tgtgccgcaa taacaccgac
     2401 cgcttcacct tggttgatta agtgaccacg agccaagtca cgtccgtaac atttagcaca
     2461 cacaccgaag tctgtatcac aagttactac cgaacgtact ttgatcacat ccacagattc
     2521 acgatccacc acatcacacc atttttcatc taataaggtg ttgcgtggaa ttaatacttc
     2581 atctgtaccc ggttttaaca catcttctgc caccacacga cctaatacac gatcacgcag
     2641 ggcttctttc acatcaccac cttcgataag tggtgtcatc acgatacctt cgtgcgtacc
     2701 acagtcatct tcaatgataa ctaaatcttg tgccacatca actaaacgac gggttaagta
     2761 accggagttc gctgttttta atgccgtatc ggcaagacct ttacgtgcac cgtgggttga
     2821 aataaagtac tgtaatacgt tcaaaccttc acggaagttt gcagtaattg gggtttcgat
     2881 aatcgaaccg tccggacgag ccatcaagcc acgcataccc gctaactgac gaatctgtgc
     2941 agcagaacca cgagcaccgg agtccgccat cataaagata ctgttaaatg aggcttgttt
     3001 ttccggatta ccttcacggt tgattacttc ttccgttgaa aggttttcca tcattgcttt
     3061 cgcaacacgt tcgtttgcag cagcccaaat atcgatcact ttgttataac gctcaccggc
     3121 tgttactaaa ccgccgttga attgctcttg gatctcagcg acttccgctt cagccgcttc
     3181 aatgattgcg tatttttgct ccggaattac catatcgtcg atacctactg aggcaccaga
     3241 acgagcagca taagcaaagc cggtgtacat aatttggtca gctaaaacca cagattcttt
     3301 taaacctaaa cggcgatagc tctcgttaat taatttcgag atcgcttttt tgcctaaggt
     3361 ttggttgaac aagctaaacg gcataccttt tggtgcaatc atccataaga ttgaacgacc
     3421 aatggtggta tctactaacg aagtttttgc ttctaattcg cctgcttcgt ttttctcata
     3481 ttcagtaaca cgcactttaa cacgagaatg taattcagcc tgaccggtac ggtatgcttt
     3541 ttccgcttca cgagggtcaa ggaagtacat tccctcacct ttaccgttta ctttctcacg
     3601 ggtcatatag taaagaccga gtacaacgtc ctgagacggt acgataatcg gatcaccact
     3661 tgccggtgaa agtacgttgt tggttgacat cattaacgca cgagcttcta actgtgcttc
     3721 aagggttaat ggaacgtgaa ccgccatttg gtcaccgtcg aagtccgcat tgaacgccgc
     3781 acacacgagt gggtgtaact ggatcgcttt accttcaatc aataacggct caaacgcttg
     3841 gatacctaaa cggtgaagtg ttggtgcacg gttaagtaga atcgggtgtt cacgaatcac
     3901 ttcggcaaga atatcccata cgataggatc ttcacgctct accattttct tcgcggcttt
     3961 aatagttgaa gcaatgccac ggttttctaa ttttgagtag ataaacggac ggaataattc
     4021 caacgccatt ttcttcggta aaccacattg gtgtaagcgt aagtatgggc ctacggtaat
     4081 taccgaacga cctgagtaat ctacacgttt acctaataag ttttgacgga aacgaccttg
     4141 tttacctttg atcatatctg ccaaagattt taacggacgt ttgttcgaac cggtaatcgc
     4201 acgaccacgg cgaccgttgt ctaataacgc atcaaccgac tcttgtaaca tacgtttttc
     4261 gttacgcacg atgatgtccg gtgcaactaa atctaacaag cgttttaaac ggttgttacg
     4321 gttaattaca cggcggtata aatcgtttaa gtcagaagtc gcaaaacgtc caccatcaag
     4381 cggtactaac gggcgtaaat ccggtggaag taccggtaat accgtcatta ccatccattc
     4441 cggtttatta ccggactgta agaatgcttc taaggttttt aaacgcttag tgattttttt
     4501 acgcttggtt tctgaattgg tttcttgtaa ctcttcacgc aaggtttcgc attggtgttc
     4561 taaatctaaa tcacgaagta atgcttggat accctctgca cccattttcg cttcaaattc
     4621 atcgccccaa cgctcttcag cgtccataaa ttgttcttcg gttaataact gatttttctc
     4681 taaatcggtc attcccggct cagtaactac ataagattca aaataaagca cacgttcaat
     4741 atcacgcaac ggcatatcta agattaaacc gatacgggac ggcagtgatt ttaagaacca
     4801 gatgtgtgcg actgggcaag ctaattcaat gtgtcccata cgctcacgac gtactttggt
     4861 ttgggttact tcaacaccac atttttcaca aattacacca cggtgtttta aacgtttata
     4921 tttcccacac aagcactcgt aatctttcac cggtccgaaa atacgtgcac agaataaacc
     4981 gtcacgttcc ggtttgaagg tacgatagtt aatggtttcc ggttttttca cttcaccaaa
     5041 agaccaagaa cggatcttgt ccggtgatgc taaaccaatt ttaatcacat caaaatcttc
     5101 gttcgatttt gtttgtgctt ttaaaaactt cactaagtct ttcacttttt tgcccctagt
     5161 ttgaggttgt tttgtttggt ggaaagaata tccactctac gaatttggtt gcgattttaa
     5221 accctctccc cttgtgggag agggatagat tttgcctcgt tcagaagcaa aatcagggag
     5281 agggtaagcg gtcgattttt gcaaaaattt agcaaattta caccgcttgc atcaccctct
     5341 ctctgccaaa ggattctgaa caaatccttt gcctgtctct cccccatcaa gggggagagt
     5401 gaaaccatta cgcttcgtcc agctccatat caattgctaa tgcacggatc tctttggtaa
     5461 ttacgttaaa ggattcaggg atacccggct ccatataatg tgtaccatct acaatgtttt
     5521 tatacatctt cgtacgaccg ttcacatcgt ccgatttcac cgtaagcatt tcttgaagag
     5581 tataagccgc accgtatgct tctaatgccc aaacctccat ctcaccgaaa cgctgaccac
     5641 cgaattgagc tttaccaccc aatggttgtt gagtaacaag gctataagaa ccggttgaac
     5701 gagcgtgcat tttatcatca accaagtggt tcaatttgag catatacata taaccaacgg
     5761 ttaccggacg ctcaaatttc tcaccggtac gaccatcata aagtgtgatc tgacctgaag
     5821 tcggtaagcc gcctaactct aataagcctt tgatttcgct ttcgtgtgca ccatcgaata
     5881 ccggagtagc aagcggtaag cctttgcgta agttttgcgc taaacgcatc acttcttcat
     5941 cagagaaagt gcttaaatca acgacttgtg agccgtgacc taaatcgtag gctttttgca
     6001 tatattcacg taatttagcc acttcttgtt gctgtttgat catcttatca atctgatcac
     6061 cgataccacg agccgctaag cctaagtggg tttctaagat ctgaccgatg ttcatacgag
     6121 acggtacgcc cagcgggttc aatacgattt caaccggctg accgttttca tcgtatggca
     6181 tatcttccac agggttgatt ttcgagataa cccctttgtt accgtgacga cccgccatct
     6241 tatcacccgg ctggatttgg cgtttcaccg caaggtaaac tttcactact tttaacacac
     6301 ccggtgctaa atcgtcgcct tggataattt tgttgcgttt gatctcaagt ttacgttcaa
     6361 attctttgcg taactcttcg tgctgttccg ctaactgttc taactgattt tgcttcgctt
     6421 cgtcttcaag ggtttgttct aaccattttt cacgagcaac gccatctaat gcagattctt
     6481 ttacgccacc ttcaattaat aggttgcgaa cacgagtaaa taaaccggct tctaagattt
     6541 ccaactcttc cgctaagtct ttcttcgctt cacacagttg catctcttcg atttctttcg
     6601 cacgtttatc tttctctacg ccatcacggg taaatacttg aacatcgata accgtacctg
     6661 aagtaccgtt tggaacacgc aatgatgaat ctttcacatc agaggctttt tcaccgaaga
     6721 tcgcacgcaa tagtttttct tccggtgtta attgggtttc acctttagga gttactttac
     6781 ctactaagat gtcgccacct ttcacttctg caccgatgta aacgatacct gattcatcta
     6841 atttgcttaa tgcagattca cccacgtttg gaatatcagc ggtgatctct tctgcaccta
     6901 atttggtatc acgcgccaca caagataatt cttgaatgtg gatagtggta aagcgatctt
     6961 cttgtaccac acgctcagaa actaacattg agtcttcgaa gttataaccg ttccaaggca
     7021 taaatgccac acggatattt tgacctaatg ccaattcacc taagtctgtt gaaggaccgt
     7081 ccgctaagat ttcaccacga gcaaccggct cacccaagtt cacacaaggt acttggttga
     7141 tacaggtgtt ttggtttgaa cgggtgtatt tgattaagtt ataaatatcg atacccgctt
     7201 cgcccggaat ggtttcatct tcatttactt tcactacaat gcgtgacgca tcaacatact
     7261 gcaccgtacc accacgtttt gcgacaatcg ctacacctga gtcaagagca attggttttt
     7321 ccataccggt accgactaat ggtttgtccg cacgcaaggt tggaactgcc tgacgttgca
     7381 tgttcgcacc cattaacgca cggttcgcat cgtcgtgctc aaggaacgga attaacgccg
     7441 cagccacaga aaccacttgt tgggttgaga tgtccatata ttgaacttct tccggtttga
     7501 ataagcccga ttcgccttgc tgacggcaag ttacataagt atcggtaaaa cggaagttct
     7561 catctaagtt tgagttcgcc tgtgcaatga tattgttgct ttcttcaatc gcagataaat
     7621 actcgatctc ttcggttact tgaccattca ccactttacg gaatggagtc tctaagaaac
     7681 cgtagttatt agtacgagca tacaccgata gtgagttgat caaaccgata ttcgggcctt
     7741 caggagtttc gattggacat aaacgaccat agtgagtcgg gtgtacgtca cgcacttcaa
     7801 agccggcacg ctcacgggtt aaaccacccg ggcctaatgc cgagatacgg cgtttatgtg
     7861 ttacctctga aagcgggttg ttttggtcca taaattgcga aagttgtgat gaaccaaaga
     7921 actctttcac tgctgctgaa atcggtttag cgttgattaa atcttgtggt gttacaccat
     7981 ctaaatcgcc taaagataaa cgctcacgca ccgcacgttc tacacgcacc aaaccaatgc
     8041 ggaattggtt ttctgccatt tcaccaacgc tgcggatacg acggttacct aagtggtcga
     8101 tatcatccac ttcaccacga ccgttacgga tttcgattaa ttttttcatc acaccgatga
     8161 tgtcatcgtt gcttaaaata ccgctgccta cgccttccgg aatttctaac gagcgattga
     8221 atttcatacg acctactgcc gataaatcat aacgctctgc cgagaagaac atattatcga
     8281 ataatgcttc tgccgcttct ttcgttggtg gttcgcccgg acgcatcata cgatagattt
     8341 caaccaacgc acttaaacgg tcgtaagttg gatcaacacg taaggtttca gaaatatatg
     8401 gaccgtggtc taaatcgttg gtaaataata cttcgatctg gttgtaaccg gcttgtgata
     8461 aattcgccaa catctccatc gagatttcca tattcgccgg gcaaatcact tcacctgaag
     8521 ataaatcaac gtaatcttga gccgctactt tgccgacaat atattccacc ggcacttcaa
     8581 tttgggtcac attgtctttt tctaacgcac ggatatgacg agcggtaata cggcgaccca
     8641 tttcaacata tactttgccg tttgctgcaa tatcaaatga tgcagtctcg cctcttaaac
     8701 gctcaggcac taacgtcatc aataatttat tatcggcaat ttcaaattta attttctcga
     8761 agaataaatt taagatttct tcggtggtgt agcctaatgc acgtagaata atggttgccg
     8821 gcaatttacg acggcggtcg atacgggcaa ataagttatc tttcggatca aactcgaaat
     8881 ctaaccaaga accacggtaa gggataatgc gtgcgttata aagcacttta cccgaagagt
     8941 gggttttacc tttgtcagaa tcgaagaaca cacctgggct acggtgtaat tgagaaacga
     9001 taacacgctc ggtgccgtta atcacgaaag taccgttatc ggtcattaat gggatttcgc
     9061 ccatatacac gttttgctct ttaatgtctt ttaccgtacc ggctgcggct tcacgatcgt
     9121 aagagactaa acgcaatttc acgcgtaatg gtgcagcgta ggttgtacca cgaatttggc
     9181 actcacgcac gtcaaacacc ggctcgccta attcgtaaga aacgtattgt aattctgttg
     9241 caccattgtt gctcacaatc gggaaaacag aacggaatgc cgcttctaaa ccttgctgcc
     9301 cttcaggatc tttttcgata aatttttcaa aagaatcaag ctgaattgtt aatagataag
     9361 gtacgttcag aacttgtggg cgtttgccaa agctcttacg gatacgcttt ttctcggaat
     9421 atgaatatgc cattttttaa gatagcctct gaaatatatt ttggttgaga ttacacaatc
     9481 cgacacgaaa gactcggttg gaagtggcat tataaaaact gtacgagatg tcattttcca
     9541 atcgccaatc aagcagtcag aaatcgctac tgcaagcggt caaattgggc aggaaatttg
     9601 caaattttgc tgattaaaat gacaaaatca gcccttgcgt tcaagcacaa aaggctgacc
     9661 ggtcaagccg tcagccaatt tttgctaaaa atggttagga attatagcgg attttgtaaa
     9721 ttttggcaag aaattgaccg cttgcaaaaa caagccccaa gattctactt gaggcttaat
     9781 agctagaaga tagcctctaa acgtacaaat gtctgaataa agtgcttacg atctttatct
     9841 ttgtcgttat aaaaactgat ttcaggctgg ataaagagcc agttgcgcca gattggttga
     9901 cgataggaga caaatggacc ccaatagtta aattttgata actctttatc taaacgcccc
     9961 cccatactga taccatagct gatgtgcgtg agtggtttga agttatgttg acgatataaa
    10021 ctattccccc aacttctttc ttcatctaca tcatgacgat attcaaaatt ggtgtggttc
    10081 ataataaagg tggtatccgt ttcggtatat ttattttcaa agttagtacg taaatagtgc
    10141 ttgctctctg aaccgtaacg ataaatttgc tctaggcggg tactgaactg gtcggtgatt
    10201 ttccaatctt tactgatgcg taaacgcccg aaaatatcgc ttcccgaacg aataccagca
    10261 tctaaatcgg tctcaatccc taaagcctta atgccatcag accaacgtaa accgatagaa
    10321 gagttcgaat ctctcgcttc acgagagtta tagcgtttat ttggattgac gtttttatac
    10381 aattttccag catgattttt atcggtaagc tcattatcca gttcgtcatc accaaatacg
    10441 acatttagat gctttttcaa gatagggagt ttgattttac cacgaacacg gggcttataa
    10501 gtaaatttat cgtgtttatc ccaacggcta tctaacatta cccgtaaaga agccgttccc
    10561 ggtttatctg ggtctacatc acctagccaa ctgttaatat tgtttgccca acgatctaag
    10621 gtagtgctta ctgtttcatg tttcttgtct acccaagtgt tattttcttc tgccatcacc
    10681 ataacaggtg taagtgctag tagcggaata agacattttg ttttcataat attctccttt
    10741 ttaggaatca catcataatc taaattattt ggtattacaa aaaatatcct ctctaggtta
    10801 cagtgcaacc tggggaaaat aaaccgctgt taatttgact aattcggata atgaaatgca
    10861 ctgaaatgca gtgagcttct cttgcaatcc tcgagcttgc acatcttgtg ccaataaata
    10921 gcaacggggc aattttgcaa aaaattgcgg acttttgacc gcttgtagca cgccatcttg
    10981 ccacagaaca accgcatcgt tctcactaag atgtgagaaa aagaagttta aggtttcttc
    11041 atcataattc gctttggaaa gggtataaag cataaatatt ccttaaaacg tcattacttt
    11101 ttcagcttga ttgagcaatg cgatcatctc agcgtgagtt ttaaattgaa gcattcgcag
    11161 taaccaatct gcattatcca gccctcgtaa acttacactt tcttggcaaa taaaacattc
    11221 agttaagtca tacaactcca ataatttgaa ggtggaaata tgatcttttt gtaagagttt
    11281 ttccggctgt tgatcggcaa tgagattaaa tacgccatca ttgataaaga ggatagctat
    11341 ctcttgctcg tcacaaaaag cactagctgc aagcaaggca tctagcccct cacggctaac
    11401 tgccgaacca aaaggtggct gggtaaataa aaaggcaagt ttatatttct tcatcttaaa
    11461 aagtcagtaa tcgatccgct tttaatactg cttggctaaa ttcgcctaac ccggctaggg
    11521 taaatccttc ggctaaatta tgtgttgcat tagctgagat ttgctcatcc accacgcctc
    11581 tacgctgggc agcagcaatg cacaaatgca gaggcaagcg gtgcaattta gccaattttt
    11641 tccacatctc gactaaattc acttcatcgc ttgctggatt cactaaggca ttagcattgc
    11701 tcacgccttc ttgaaaaaag aatatttggc aaattgtatg attggctttt aataattctt
    11761 ccgccacttg gtaggctaaa aatgcccctt gtgaaccata aaccggtgat tttatcgcta
    11821 atacataatc cattaatcat tgtcctgctt aatttgacga atatagagat acacagtatg
    11881 gcgtgaaata tccagcttct ccgctacttg gttaatggaa tctttaatat caaaaatccc
    11941 tttctcaaat aacgaaatca caatttgacg atttttatta ttgttagaaa tttgacgatc
    12001 tgccatcaca tcatcaatgg tcgtttgaat cgtttgtgaa accaaatctt ctactgaact
    12061 ggcaaaagta acttctgaag cggtatctac aggggcaata aaagaattga gaaactgtga
    12121 ggtaggtact tccaaattaa tattaatgca cagtaacccg ataacgtgct gtttaccatt
    12181 acgaatcgca atagtgatag acttcattaa aacgccacct tttgccttgg taaaataagg
    12241 tttggagacg ctatccgttg ccatatgatg cagcgatttc aatgcacggt cagtaatcgg
    12301 agagccgatt ttacggttag tattgtgtcc gttagcaata taaattgccg aatgttccag
    12361 aaactctaac gaatgcaata caatttcgca atgctcacca atcagtgccg caatcccatc
    12421 taccatcgga aagtaagaag caagaatggc gtgatcttcg tcagaaaaag gcgtgaattt
    12481 tttggtcata atacaaatta caagcggtta gttttatcac attttttgca aatattataa
    12541 taaatctaac cgcttacgta tcttcaataa ttaaggaata acgaattatt tcttagcatc
    12601 tgctgcttta aaatctaata attcaatttc aaatttcaag gttgaatttg ctggaatttt
    12661 gcctgcggca cgatcaccat aagctaatgc aggtggcact acaatttcca tcttaccgcc
    12721 ttttttcaac ataggaatcg cttcaatcca acccggaatt aattgattta actgaaattc
    12781 aatcggctca ccacggtcgt aagagctgtc aaataccgtg ccatcagtca aagtgccttt
    12841 gtaatgcacc ttcacagtat cttccgcttt tggtgaattg ccttcacctg ctttttcaat
    12901 tttatataac aggcctgaag ccgtttcttt tacgccagac tgttttttat aatcagcacg
    12961 gaatttatcg cctgcagcta ccgttgcttt ggttttttct tcgttaatct ttgcttcttt
    13021 cgcagataaa taggcatcta acgattttaa ctgcttttgc aaatcttcat cggttaattt
    13081 accggttttc tttaaggtgt cctgaacacc ggctaagatt ttatcctggt tataggtaaa
    13141 aatatccttt tgtgactcaa tcacaccatt aatattttta cccattaata cacccacggc
    13201 ataagaggaa tcatccacaa atttaggatc tgctttttct tctgccatca catttgtact
    13261 aaaaagtgtg ccaacaataa gagtaaataa tgataatttg gtttttaaca taatttttcc
    13321 ttataaaaat aactgttaag aaaatttaag atagggtaaa gtgtaattcc ttttcacaca
    13381 accttttttt agtaaaaaat ggataataat tatacattat cggcaaaaat taccgaattg
    13441 gagactaaag ttgctttcca agagctgatt attgaagagt taaatcaagc acttattgag
    13501 caacaatttg caattgataa gttacaaact cagattcgcc atattgctga gaaattgaaa
    13561 ggggctcaac taagccaagt tgcttcacaa gcagaagaaa caccccctcc tcactattaa
    13621 tgctgttaaa atagcgtttt tttcaaaaaa taaacaatac attagagaag gaaagtaatg
    13681 aaaattggta ttattggtgc gatggcacaa gaaattgaga ttttacgcca agtaatggtt
    13741 gaacctaaag tcactgaatt agcaggttgt aaaatttttg aaggcaaaat taacaatacc
    13801 cgtgtggcat tattacaatc gggtatcggc aaagttgctg ccgccatcgg cactacctta
    13861 ctgatacagc tcacccagcc ggatatgatt attaatacag gctctgccgg tggattagat
    13921 ccagatttaa acgtaggtga tattttgatt tcaaccgaag ttcgttatca cgatgttgat
    13981 gtgaccgcct ttggttatga aatcgggcaa ctaccggcaa acccgccaac attttcgcca
    14041 aacgagcaat tagtcgaatt agctcaaaag gaagcgaaaa aagccggtta caatgtggtt
    14101 gcaggcttaa tctgtagcgg cgatgccttt attaacggta atgataagat taatcaaatt
    14161 cgtcagaatt tcccgaatgt cgttgcagtc gaaatggaag cagcagccat tgcgcaggtt
    14221 tgtcacggtt ttaaccttcc atttgtaatc gtaagagcag tatcggatgt agcggacaaa
    14281 gaatcccacc tttcttttga agaatttctg ccattagccg cagaaaaatc ttccacgatt
    14341 gtattagcaa tgctaaataa tatcgattaa ttttaattgt tagcgaaatt tcaagtttgt
    14401 aaatgtttat taaaaattga ccgcttgtta tcgctatttt gggcaagatt ttgtagaatc
    14461 ttgttagttt tttgcctaca gacaaaaatt gtaggcattt ttctttttag ttattgagag
    14521 ttatatgagt gcttcaccaa atattggttt tatcagctta ggctgcccta aaaatttagt
    14581 tgattccgaa cgtattttga ccgaactgcg tgcggatggt tataacatca tccctagtta
    14641 tgaaaatgcg gatcttgtga ttgtaaacac ctgcggtttt attgattccg cagtacaaga
    14701 atctcttgaa agcattggtg aagcgttaga agctaacgga aaagtaattg tcaccggctg
    14761 tttaggggca aaagaaaacc aaattcgtga ggtacaccct aaagtattgg aaattacagg
    14821 gccacatagc tatgaggcgg taatgaacca cgtccataaa tatgtgccaa aaccggaatt
    14881 taacccttac accagcttag tgccaaaaca agggattaaa ttaacaccga agcattacgc
    14941 ttatttgaaa atttccgaag gctgcgatca tcgctgcact ttctgcatta ttccttcaat
    15001 gcgtggcgat ttagcaagcc gttctatcgt gcaagtatta gatgaagcaa aacgcctagt
    15061 tgatgccggg gtaaaagagc tgttaatcgt ctcacaagac acctcagcct attcgttaga
    15121 tcgcaaaaaa gaagaagaaa ccaaaaccgt tttctggaac ggtatgccga ttaaaagtaa
    15181 tttaattacg ctatgtgagc aattaggctc aatgggcgtg tgggtgcgtt tgcactatgt
    15241 ttacccatac ccacacgtag atgatttaat tccgcttatg gctgccggca aaattttgcc
    15301 gtatttggat attccactcc aacacgccag cccgaaaatc ttaaaagcga tgaaacgccc
    15361 gggttcaatc gaaagaacct tagaacgcat taaaaaatgg cgtgaaattt gccctgaatt
    15421 aactctgcgt tctaccttta ttgtcggctt cccgggggaa acagaagaag atttccaact
    15481 attactcgat ttcttaaaag aagcacaatt agatcgtgtt ggttgcttta aattcagccc
    15541 agtggaaggt gctgttgcta ccgatatgcc ggatcaagtg ccggaagaag taaaagagga
    15601 acgtttccac cgttttatgc aactacaaca agaaatttcc gctgctcgct tacaagcaaa
    15661 aatcggcaaa accgtacaag taattattga tgacattgac gaagaaggca ttatcggtcg
    15721 ctcaatggca gatgcaccgg aaattgacgg tgtagtctat gtagataatc cttcaaacca
    15781 attagtgaca gtagggcaaa ttatttcagt aaccattaca catgcagatg agtatgatct
    15841 gtggggaatt ttaaacaact aaggatacat tatgtcagca gaaaatatgc ctagcgtcat
    15901 ccgttttgag caggcggtcg cgaaaaaaga ttatgaatcg gcttgtaccg agctactgtc
    15961 tatcctttca aaattagata gtaattttgg tggcatttcc aatattgaat tgaatatgcc
    16021 agagcagatt gaaaatttag aaaatgataa agctatctat ttctgtactc gaatggcggt
    16081 agcgattact cgcttgtttg aagatcctgc attagagatc tccgaacacg gtgctatgcg
    16141 ttttctgaca ttacaacgct ggatagcatt aatttttgcc agttctccat acgtaaacgc
    16201 agaccatatc ttgcgtactt acaatagaaa taaagaatct gctaatccaa atacggttga
    16261 tttagatgcc acattacaag cactaattaa gttctgtatt ttatacttac cggaatctaa
    16321 tattttattg aatctagatg ctgcatggaa tgcttcatca gatctcaccg catcactttg
    16381 ttttgcactc caatcaccta gatttattgg tacatcctct gcttttgcaa aacgcgcggc
    16441 tattttgcaa tggttcccag aaaaattagc gcaaattgaa aacttgaaca agctaccgag
    16501 cgccatttca cacgatgttt atatgcattg cagttatgat attgaagcaa ataaacataa
    16561 tgtaaaacgg tcgttaaatg ctgtaattcg tcgtcattta ctttctgtcg gttgggaaga
    16621 tagaaaaatt gaacagcttg gtaccagaaa taataaacct gtcatggtag tattattgga
    16681 gcatttccat tcatcacatt ctatttatcg tactcattca acctcaatgg ttgctgcacg
    16741 cgaacatttc catcttattg ggctaggcag tgatgctgta gatgaaatgg ggcaacaggt
    16801 attcgacgaa tttcatttat taccacaaga tggctcacta tttgaccgct taagcttttt
    16861 aaaagatatc tgtgataaaa ataatccagc cgtattctat atgcctagca ttggaatgga
    16921 tttaaccact attttcgcta gtaacactcg cctagcacca attcaagctg ttgcgctagg
    16981 tcatccggca actacacact ctgattttat tgaatatgtc atcgtagaag atgactatgt
    17041 tggttccgaa agctgcttta gtgaacaatt attacgtctt ccaaaagatg cgctacccta
    17101 tgtgccttcc gcattagcgc ctcaaaatgt ggtctataac ctacgtgaaa atccagaggt
    17161 tatccatatc ggaattgcat caaccactat gaaattaaac ccatatttct tagaggcatt
    17221 aaaagccatt cgtgataggg cgaaggtaaa aactcatttc cattttgcat tagggcaatc
    17281 tagtggaatt acgcatcctt atgtggaacg ttttatcaaa agctatttag gaaatgatgc
    17341 aaccgcacac ccacattcgc catatgatga atatctaaat attctacata attgcgatat
    17401 gatgctaaat ccattcccat ttggaaatac aaacggcatt atcgatatgg ttacgcttgg
    17461 tttagttggt gtctgtaaaa caggccctga agttcatgaa catattgatg aaggtttatt
    17521 taagcgcctc gggctaccta attggttaat tacccaaaca gcagaggaat acgttactca
    17581 agctattcgt ttagcggaaa accacgaaga acgcttagca attagacgcg acattattga
    17641 aaataataaa ttacaaacat tattttctgg cgatcctcgc ccgatgggac aaatattctt
    17701 agctaaagtg caggcatggt tagcggataa aaaccctaaa aatgcggaag tagaagttaa
    17761 aacaaagaaa gtaagaaaag cagctactgc tagccaatcc gcaaaaaaac agacaacttc
    17821 gaaaacacag acagccaaag cggaaaagga taacgcagca aaaaccgaaa ctaagagcta
    17881 gttcattaaa caaagcccaa gtagtcattt aacttacttg ggctttttgc aaaaaagtga
    17941 gtcaatttaa ccgcttgtat caacctaatg cggcaccact taaaccaata aagaaacccg
    18001 ctatagttgc actcattaag ttcgccaatg tgccgccaat cactgctttt aaacctaaac
    18061 gagcaatatc tgaacgccga cttggtgcca tacttccgat accactaatc aataccgcaa
    18121 ttgcactaaa gtttgcaaaa ccacataaag caaaagtaat tattgcctta gttttatcgc
    18181 ttaattgcac cacagcatca ggctgtagat atttagcaaa ctcaagatat ccgacaaatt
    18241 cattaacagc aagtttcata cctatcattt gacctgcaat atcggcatct tgccacggca
    18301 caccgattaa gaaagcaagt ggttggaata aataacctaa taaaatttgt aaggttagcc
    18361 cctcataacc aaaccaatta ccaatgccac caataaagcc atttaataaa gcgatcactg
    18421 caataaatgc tattaacata gcaccgacat taatagccaa agtaacacct gcactagcac
    18481 cattagctag ggcctcaacc acgttatttg gcttttcaac ttcaacattg tcatctgctt
    18541 tactctccaa ggtttccgtt tgaggataca ttaatttagc aaatagtaac ccagctggtg
    18601 cagccataaa ggaggccgca ataagataag ttaaaggaac gcccattccg gcataaccag
    18661 ccataacgga accagcaatt gaagcagttc caccaaccat aatggcaaat aattctgact
    18721 ctgtcatacg gctaatgtat ggttttacaa ctagaggagc ttctgtctga ccaacaaaaa
    18781 taattagctg ccgctgacat tgattctgcc ttggaagtgc ctagtgtttt ttgtaatgct
    18841 ccaccaataa ttttaatcac ccattgcatc acaccaaggt agtaaagcat tgaaattaac
    18901 gcagagaaga agataataat aggcaatact ttaaccgcaa acacaaaacc aatgctgcta
    18961 ttactcggat tcgctaaatt gccaaacaca aagctaatac cttcgttacc ataattaata
    19021 atattgctca cacaatttgc agtagctagc aatgcatttc ttcccgctgg aaagtaaaga
    19081 ataagagcgg caaaaccaat ttgaatcgcc aaagcgccca aaattgtgcg aaatttaatg
    19141 gctttgcgat tatttgataa taaaacacct aagcctaata acacaaaaac accaattatg
    19201 ctaattagta tatccatcgt aagtctccat tagtaaatag taataacttt ttgaataccg
    19261 tgccgttttg caaatgctgc cagttcatcg gtttgctcga tagtgggtac aaaacgagcc
    19321 aagcctgttt catctctcgc tcctttggtg tgtacccgaa taattttaag tagtacatct
    19381 gggtaaggtt taagtaggtc agccactttt tcgagcaaat ggtgggcgtt ataaaactca
    19441 ttgaggtaaa ccaaacgtac ttcttccacc ttatcaagcg gtaataattt ggctaaattt
    19501 tgcaaattgc gagccaagtt ttcaggttta atatgattca cgtttggaag agcctgttca
    19561 ggcactttgc cggcttggtt tttccgatca aagcatagcg tttgtaagcc cgccccatct
    19621 cctttgagat cgaacagaaa tttatcggta acattaatta acggctctgt tgcctcaaaa
    19681 tcaaaaaagc cgctgctgtc taaataacag gttagccctt gctcacgcaa cgcctcgaac
    19741 agtggcacta attttttatg atgaatggtt ggctctccac ccgaaatagt gactcctcga
    19801 ataaagggaa ctgcctccat cacttgatcg tacaagtatt gcaagctaac ctgttttgct
    19861 tccttgctat aacgtggaat ggtttccggg ttatggcaat acaggcaatt cagcttgcaa
    19921 ccttgtagaa aaatgctggt acgattgccc tgcccttcta cattcgagaa ggggataatg
    19981 cggtgtaacg gcacaaaaat ctcagaaagt gcagccattg ttaccctgca ttcacatttt
    20041 cttcatcacg cagctggcgg tcgaacactt tcgcacaatc atcggtgcct gaaccatacc
    20101 aagtggtatc acgcaatacc gcttcgcctt tgcgataacg ttcaacctcg ctttttttca
    20161 ccaagtagcc tgtcactcga atcaaatcag tatttttcag gtaagtggta atgtagcgat
    20221 aaccttgggc gaacgagccg tcaataatat ccaccaccgc atctaaatga tcagtgtagg
    20281 tttggtcaaa ggcgaataaa tcccccgtac cggatgggaa atatttatgg aaaggagcag
    20341 actgttttaa gtgtgccaat aaggttggct cttcgcccac acgaatgcgg tgtgccggcg
    20401 tgttcatttt gtcttcagcg tggttatttg cccccacttg agcgtgtaac agatattggt
    20461 tattggttcg ttcaacatac agccctttat gagcgtcatt tactgcttgg agcttatcca
    20521 taatcagcgt cgcaatttca tcgccacgtt tgctttgccc aaaggtttca tttaaacctt
    20581 cttgcttgag taaatgattc accgcatcgg caagacctac aatcgcaatc atacttgtga
    20641 agttagtacg tttgataaag ccctcctgaa ccaagaagct actttcaaag aaattgcttt
    20701 cttccaccaa gaatttatgc cgtttatcca tcgtggaaag ggcgagtttt gccactttcg
    20761 gcagcatctc attgaccatt tgttcaacac tcgcacaagc acgcccgata gtacctaaac
    20821 gcaggcgggt cagagtgtaa gcaccgccac attccggtaa ggcgttataa cagcttgcaa
    20881 ttccgtattc ttcgcctaaa tcttgaatgt agtaggcatc attagcaaaa gacggtttgg
    20941 acaccaacaa gcacgctttc gccgccagtt cggcaaattc acgcgatgtt ttggttttat
    21001 cataacgaat cgtcatattc ggtgtagtgt tttcaagctc aatcaccgct tttaagatta
    21061 aacgaccggc tttggtgtca ttcggcccga tgttagcgtg gcagaaagaa tcggaaatgg
    21121 ttttatcgat atgatttaag aaacgcttga ttttaatgta atcttgctct tcatcggtta
    21181 taaacggttc taacagttgg tctaaacgcc cgatatacac cggaaaagta gtaatggacg
    21241 gaacgtgtga atacagaatt aacaagccgt ctaaggcttc atctaaattg gttggcggag
    21301 gtaaatctaa gaattggcaa cctttttgca tatacacatt ataatcaggc aaaatataac
    21361 gtgggcgata aatcgcataa ccttcattta aatcgcaaat catctgattt tcaatgtgtt
    21421 cccactcttc ttcagtgtag ccaagtagtt caatcgggtt aaataaccgc tcagcaatat
    21481 tgcctaaact catcaatttt tgctgataag tgagattttt tgcttttact acctcaagaa
    21541 tatcttgtaa tgaagcctgc ataacatttt cctctctatt aagtgatagt tcggatttta
    21601 gtcaaaccga ataaaaattg ctgtgactta gatcacagtt tctcttttat attcctcaaa
    21661 agcataaggc aaccgattgc gttaggtaac agcaatttgc aaatttttga ctttttctga
    21721 ccgcttgttt taactcagac tgattaaggt gtaaaatacg ccacaattta agcaaaagga
    21781 cagactgatg gtacaaaaac cacttaacca ctatttagaa atgcaggagc attttttata
    21841 tgttccctat ttacaccatc accgccgcat tcgggtacta ttgccgaagg attatcacaa
    21901 cgaaggttgg gaacgttatc cggtgttata tatgcacgac gggcaaaatg tgttttacag
    21961 caaagagtct tattcggggc attcttggaa aattattcca accattaaat cacaccaaga
    22021 attaccgaag atgattattg tcggcattga taacgcagga gctaatcgct taaatgaata
    22081 cggcccgtgg aaaacaacca ccggctcaac ccctgaaacc cgaaatgccg gaggcttagg
    22141 ggaggaatat gccaaatggg tggtggaaac ggttaaaccc tttattgata ttcactatcg
    22201 caccaagcct cagcaagaac ataccttatt ggcaggcagt tcaatgggtg gcattatcac
    22261 cgcttatatg ggcagccgct acccacatat tttcgggcat ttaggggtgt tttcgcttgc
    22321 ctcttggttt agtgagccgg attttctgcg ttttacgcac caatatccgt tacaacccaa
    22381 taccaaagtg tttattcagg ttggcactaa cgaaggcgat gaaatcgatt cgcactttat
    22441 ctctaatacg aaccaaacct atattgattg ttcgctcaat tactaccaag cactgattcg
    22501 gattggcgtg ccgttagaca atatccgcct gcggattatg gcaaatgaaa tccaccacga
    22561 aatgcactgg gcagatcact ttgtggaatt tttacatttt tcactgctgc gcaagtaatg
    22621 gataaaggta atcatatcta cattacacaa ttctgaaaaa acaaaacccc gatgtttcca
    22681 tcggggtttg ttgaacttga agttctgaca aaataaatca aaaattattt gatttctact
    22741 ttcgcgccag cttcttctaa ttctttctta agtgcttcag cttctgcttt agagatgcct
    22801 tcttttaatg cagctggagc tgattctact aagtctttag cttcttttaa gcctaaacct
    22861 gttgcaccac gtactgcttt gattacagca actttgtttg caccagcttc tgctaatact
    22921 acatcaaact cagttttctc ttctgctgcc gctgcgcctg ctgctggagc tgctgctact
    22981 gctgccgctg ctgaaacacc gaatttttct tccatcgcag tgattaattc aacgatttct
    23041 gatactgatt tagaagcaat cgcttcaatg atttgttcgt tagttaatga cataacaatc
    23101 gattcctaaa gtaaaataag ttgttaaacg aataaagaag agtgcttaaa attaagctgc
    23161 tgcttccatt tgatcgcgta atgctgcaag agtgcgaaca agtttgcctg ccgcagcttc
    23221 tttcattgtg cccattaaac gtgcaattgc ttcttcgtaa gttggtaagg ttgctaagaa
    23281 tgcaacatct tgttttttac cttcaaaggc tgcaccttta agttcaaact ctttgtttgt
    23341 tttcgcaaat tcagtgaata aacgtgctgc tgcacctggg tgttcatttg agaatgcgat
    23401 aagagtcgga ccggtaaacg tatctgttaa gcactcgaac tcggtgcctt ccactgcacg
    23461 acgtaataaa gtattacgta caacttgcat tgtcacacca gcttcacgag ctgctttacg
    23521 taactcagtc attttttcaa ctgttacacc gcgagaatcc gcaacaacag ctgaaagtgc
    23581 acctttggca gcttcgttta cttcagcaac aatcgcttgt ttgtcttgaa gatttaatgc
    23641 cattggtttt agctcctgat acactctgcc ttttcaagca gaatttacaa aaaatcccaa
    23701 aaatattacc gcttgcattg ctacaaactg caatcttctt tgagcctaag gtgtccagaa
    23761 gcagagaatt ctgtctgttc accatctatg taggattatt aagttttcac ccctacagtc
    23821 ttagacgggg cttagatagg ctaagcacca tcagcttgct ctcgcaagtt aaggtcgcaa
    23881 attatacaga taatctgcga ccctgtaaag cacaaaatca aacttaatcg ccaatttctg
    23941 ctttaccaac aacttccgcc aatgccttca accgtgcatc agacttcgct acaaacgggt
    24001 tgttttcgtc ccaagcgtag caagcgagaa tagaggagat catttggcgg atttctggtt
    24061 gcggattgcg ggcgtagatc actttgcgtt cgccgttgaa gattttgctg ttgtcttggg
    24121 gaatgtcatt gatgtccata cgttttccga tgattgttgg acgggaatag cccccgtccc
    24181 tacgatttta cgaatttacc accgcttcca aaagcataaa acaagacgaa aaacgcccgc
    24241 tttccggcac atagcagagg attttttgtc cctgtttgag atcgtgggtg cggataaatt
    24301 catcgagcat aatgtagatc gaggctgacc cagtgttgcc tttggtagtc aggttggtga
    24361 accatttttc ctgtgggatc aggaaattga tccgctgtaa accttccaat aatttatctc
    24421 ggaaggaacc ggacgagtag tgcggcagga agtagtcaat ctcttcagcg ttgagcttgt
    24481 atttcgggat aatccgctcc aatgcttttt ccaccgtgta tttgacgatg ttttcgttga
    24541 gcagtttgac atcttgtttc accgccattg ccgagcattc ggcacgttgg gcttcgctca
    24601 ttgctttcca gccggtgtag ttgccgtcaa taaattccgc accggcgtac atacaaaccg
    24661 gcatttcgtt agcgtaagag accagatcga tccagtgaat tttgaggctc aacccttttt
    24721 cgttcggctg attggcaagc aataccgccc ctgcaccgtc tgagagcatc caacgcagga
    24781 aatctttctc aaaaccgatc tcagggcgag catcgtccag ttttttatga tcgatttcgg
    24841 cacggaaatg ttcactgcgc ataatggcgg aggcggtttc ggaagccgtt gcaatcgcag
    24901 cacgatgctc accgatgcgt acggcattat aggcgtgctt catcgctgcc attcccgcca
    24961 cgcacacacc ggcagtggtc agcacttcca tcggttcggc atcaagcaag ccgtgtacca
    25021 tcaccccctg ccccggcata atttggtcgg ggtaagatgt ccccaccgcc aagcaagtcg
    25081 ctgatgttgc agaatttccc aattttttga ccgcttgcac cgccaactcg gtactgctgt
    25141 gagtcgcttg gcgggttatc gggtcaatgg cataataacg atattgaata ccgttagaac
    25201 gcaaaatcat cttccgcact cgggaaggcg tttcccccac cattcccaac accgcttcca
    25261 ttgcatcgtt ggacacaggg gcgttgggta aaaaggcgga ggtgttggtg atatagacgt
    25321 tagatagcat aaagtttccg tgtagggtac gtttactcgc ccgaaggctc ggcaaaatac
    25381 cgtttttgtt tttctaattt ttctttcagc agtgggcgta atagccgttt aatcaccgtt
    25441 gaaatcggga cgacggttaa aattaacgtc actaaaaaga ggatgtaaaa atagagtacc
    25501 gcttggcgta agtgcggtga aatttgaccg cacttgatca gtaatttgcc ccaaaggtgg
    25561 aaacttctcg caccgacttt ttcgctcatc atcaattttt cgtcaatttt gaccgcttgc
    25621 atttggcgga aaagggtgct gtcaagcggt tggttttggc taaaaatgtg caaaagtttt
    25681 gccccgaagc gttttacatc ttccatttcg ctttcggcaa tgccagcgct cggcagcttg
    25741 ccgatttttt tcttgccggt cagcatccaa agcggtgtgg tgataaaact cgcccagtcg
    25801 ttagattgat ccactttgac cacgttgcca atgagcttcg cctcactctc ggcaagcagt
    25861 tttttcactt tttcctgcgc catcagccac atattgcgac agccgatcag cgtgatgacg
    25921 ggcgtatcct tcaacacttt ttttgcctgt tcactctgta aaaaagcggt aatcggctgg
    25981 ctgggggaga gaaaccacac ggaataggcg atgatcacca aatcgtatat ttcgtgctga
    26041 aagggcagag gctcaatcgg cggcggtgtg aggtggacgg tttcggggaa ggtgttaaaa
    26101 aaacgcataa accgccacgg aaacgggtat tcctgttgcg gtttgattgc gagtttttcc
    26161 acaaaaacgc ccgcttgttc aagcggttgt acaaaatttt ctgccaaacg ggtgagttgc
    26221 ccggtttggg agtaataaac taataatatc tttttcatta tttgaaaacg gttatttcca
    26281 atgtttaaaa atcaatgagg tattcatacc gccgaaggcg aaattgttgt tcatcacata
    26341 atcggtgtgg atttcccgtc cgtcaccttg aaggtaatcc aagtcgccac agcgttcatc
    26401 gacattttct aaattcagtg ttggggcgaa ccagccgtct cgcatcattt caatgctgaa
    26461 ccagctttcc aatgcaccac acgcaccgag cgtatgccct aagtagcttt tttgcgagct
    26521 gagcggaatt ttgccgaaca ccgctgtggt ggcttgagtt tcggcaatgt cgccctgctc
    26581 ggtggccgtg ccgtgtccgt ttacatagcc gacttggttc ggttgaatac cggcatcttt
    26641 aagggcaagt tccatacagc gttgcattgt ggtggcttgt gggcgggtga cgtgggaacc
    26701 gtcactgttt gcaccgtagc ccaccacttc ggcgataatg tttgcccctc gtgctaacgc
    26761 gtgttccagc tcttccaata cgaacattcc cgctccctcg ccgatcacta agccgtctcg
    26821 gtctttgtca taggggcgtg gcgtgccttt tgggttgtcg ttgcggcggc tggcagcata
    26881 gaggctgtcg aagacataga cttcggaggc acaaaattct tctccaccgc cggcaagcat
    26941 catcgggatc ataccgtatt taatggcttc gtaagcgtaa ccgatacctt ggctgcccga
    27001 ggagcaagcg ctggaagtcg ggataatccg ccctgtcagc ccgaagaaaa tgccaatatt
    27061 ggcagcggtg gtgtggggca tcattcgcac ataggtgttg gcattgaaac cgtccgattt
    27121 gccggtcatc agcaattcgg cggcatcttt gatgtcattg gtagagcctg tggacgagcc
    27181 acacgccacg cccattcgcc cgtcttttag ccagtcgaaa atcacgccgt tttcgtccaa
    27241 caaaccggcc tgtgctaaag cccgctcgct ggcctccacc acaaattgcg acactcgccc
    27301 aaggctgcgc aactgcttgc ggttccaatg tttcggcggt tgatagccgt gaatttccgc
    27361 ccactcttta ccaaaagcgg taaccgcacc aattccggtg atgacgactc gtttcatatt
    27421 tatctctttt tggcttcggg cgggtactgg atccgcccct acgaattatt atcctaaagt
    27481 taacacaacc caccattcac agcgatgact tggcgtgtga tgtaagcggc tttttcatcc
    27541 attaaaaacg caacggcgtg tgccacttca tcaggatcgc ccattcgtcc tgcggggatc
    27601 attttgagaa tttcttcgat tgggacgttt tcgtccagaa tatcggtatc aatcaagccg
    27661 ggcgccacgc agtttaccgt gattttacgt ttggcaagct cgatggcgag tgcttttgcc
    27721 gcaccaataa tgccggcttt tgaagcgctg taattgacct gcccacggtt gccgatcaag
    27781 ccggaaaccg aggtgataca gacaatcctg cctgctttac ggcgacggat catcggcatc
    27841 ataatcgggt gcaggacatt ataaaaaccg tctaaattgg tacgcaacac gctatcccaa
    27901 tcctcatcgg ttaaagcagg aaaggcgtta tcacgggtaa gccctgcatt tagcaccacg
    27961 ccgtaatagg caccatgttg ttccacatct gcctctaaaa tcgaccgcac tttttcacga
    28021 tccgccacat caaactgtaa cactctggca ttttttccga gatttctgac cgcttgtgcc
    28081 accgcttccg cttcttccac acgagaacgg caatgtacca caatatcaaa cccttgttgt
    28141 gcaagggtta aagcaatcgc tttaccgata ccacggcttg agccggtgat gagaactgta
    28201 ttattcatct tatatttcct actatttctt gagtgtgtag tattgggttg gatcttttgg
    28261 acttaagccg tgccaagtga gaatattttt ttgttctaaa ctttttaata ttctacgaga
    28321 taattcttta ctccgatcta atatctcact agcaatcttt acagtaatca tttcattttc
    28381 atataaatat tgcaggatag taacttcatt tttatttaat gtcgccataa tatcctgcgt
    28441 aaaaatttct tctattcgtt ctaatgccct taatgtccga tgttcaatat tattttctaa
    28501 aatcagtctg accgcatttt cattgggttc aaagtaagaa ggtgtattaa gttggtaagc
    28561 gttcatttcc tccgccatac gattaacacc ttcattcagc tctttgaccc aaccaaattc
    28621 atacagcact cgtgcaatct tcggattgcg tgaaaaacgg gtatagtaca tattttcaag
    28681 cgtcacaggg cgaggtaatc cacccggact aaaaatttcc aaacggtcat caaacatcga
    28741 aatgcgaatg cagtcccctt gattactgta actacggtgt gcaatcgcat tgacaatacc
    28801 ttctaaccaa gcaaattctg gatactcatc aatttttgtg aattgcccgt tttcatctaa
    28861 acgttggaac tctcgtaatt ggctttttaa tactgcctta gcactctcca ataacttagg
    28921 aataggctct tcaagcataa attctttgac gacattaaaa gttctaccgg tcagaggtgc
    28981 tttaccttca taccgcaaaa aacgcaaacg ggcattagga aaataacgtg tcgggttatt
    29041 cccaaatagc aaaatacagg cattcgtaat ttgcccttct ttgttaatta aatctcgggc
    29101 tttcagaatc tcttctattg agcgatctgt gtgtaatttc tgtcgatact gttctaataa
    29161 atccatatcc agatcagtaa aatcagcgcg tgctatcggt tgctcttcaa aagcacgctg
    29221 ccctttttcg tactctaatt gcaaacgttg ttcaaaattg agttccaaag tttgatcttt
    29281 tgagcgtaga aaaacccgat catcgtgaga ggtaatcgcc ctcccactcg aagctgtaac
    29341 ttcaaataat aagacaaaat ccgctaaacc tcgactattt accacctcaa tttgctgata
    29401 ggtcaccggt atcggcattt tttgtaaatt gtgcagagcg tggataaatt cttccggctg
    29461 atgggctttt tggtagttaa aaccggtaat ttcgccatta tcttcaatac caataactaa
    29521 taaaccgccg tctgcattcg caaaaccaat caaatgccgc aaaatctctt tcggttcttt
    29581 tctcgcactt ttgcgatcaa aggatttatc ttctttgcga gtacaaagat gttgaatgtc
    29641 ataggcctct tgcataacga atttcctacc aactactacc aactactacc aactactacc
    29701 aactactacc aactactact tactactact tactactacc aactctattt cggactaaac
    29761 acattcagcg ccccttctaa aagcacttgg ttgtcggcta aatcgatcag ttggcagtcg
    29821 aatacgccga agccggtggc atcttgaata gacattttga tctgaatttg tagctgtgag
    29881 ccaatcggga tttcttggcg gtggaggtgc agtttgcgag tgccgagcag gtagcctaag
    29941 cgaactggtt cgccggcttg tgtggcaata cagccagccc acgccgccac gccttgtgcc
    30001 ataatttcaa tgctggcgta ggtggcaagt tttccgtgtt tgatcagcgg attgtcaggg
    30061 cgaatattgg tttcagcggt caaaaaatcc tcaccgaatg cggtaatgcg gtcgagtagg
    30121 atcatattgc cactgtgagg aaccaatggt gcaatttgtt cgatagggaa ggtgaattta
    30181 tgcatcgctt tctcccaaaa tcagtacgct gttattgccg ccaaaggcaa aagagctgct
    30241 tgcgccaatc cgtctgcctg ctgcccaacg gctctgttca taggtgatcg caatgttcgg
    30301 caggttttcg tccgcttgtt tatcccataa ttgcgcaggg agcttgccgg tcgggttgtg
    30361 ttggcgacag agcattcccc acaaaattgc cgcttccacc gcaccggcag ccccaagggt
    30421 atgcccggtg tagggtttgg tggtagtgca agcggtctga ttaccgaaga tttttgccac
    30481 agcgaggctt tccatttgat cgttatgaag cgtccctgtg ccgtgcaggt tgatccagcc
    30541 gatgtcggct gcgtctaaat cagcattgtg cagggcattt tggaaagcgg aaattgcccc
    30601 ttccccttcg gggtgaggtg aggacatatg gtaagcgtcc gagctggaac cataacccag
    30661 taactgaatg cgatgttcgt ccagtggttc acggctcatc acaaacaccg ctgcgccttc
    30721 gccgatgtta atgccgttac ggttagcgga aaatgggttg gtttgttggt ttgataacac
    30781 ttccagcgag ctaaatccgc tgatagttaa aggcgaaagg gtatccacac caccgcaaat
    30841 caccgcatcg cacaagccgg ctttcagtaa acgggcagca gaaattaacg ctcttgcccc
    30901 tgaggtgcag gcggtggaaa tgccgtaact cagccccgtt aagccataca cttcggcaac
    30961 gaaatcagac ggcgcagaga agagaatttg ctgttgatta aactgctcgg tggcataatc
    31021 gccctgcaca ccggctttga agacgggaat attttcatcg gcgccggtag tggacgtgcc
    31081 gatgatcacc gcaatccgct cacgcccgaa ttgctcgata caagcggtaa ttttcggctc
    31141 aatttctgca agggcgtgcc atagcagttg gttattgcgg ctgcggtgtt caatagatag
    31201 ttcaacagga aaatcacgca atctagcaga gacttgccct agaaatttcg gtgaactatt
    31261 tgaaatatta tgtcgtaaat aaggctgtga agtcgtatct aaaggagata ctttcgcatt
    31321 aattaaaaca ttcaaatgct ccacgatgcc atatccaatc ggactggtga tggcgggctc
    31381 tgataaatat agtgtcatta ttatttattc cagtattatt gtgtaatggc actaaaatta
    31441 taaaattcat attcaagtga ttcaagcata gaggttcaag ttttcgctta ttcccccaat
    31501 atttccactt tccatttact gctatctggt agccaaatcg taaattggga tttttccctc
    31561 tcaattttcc aacctttatc taaataaaaa ggtggagaat gagggtttaa tgcattgact
    31621 aaagctgtaa ataatagctg tgcctgctta tttggcatta caaagccatc tggctcccaa
    31681 ccttttttag taagccatac tctggcaata ggagcaccta aaggatcagt ttgaatccaa
    31741 cgccatttat tttcataaaa ttgaattaga agcaaacttt gctggccatt attttcaact
    31801 ttaaaggatt ttacagattc aacctgtggc ggtaattgct taatttgagg taatacagta
    31861 caagcactaa gcaaaaaaat aaagcaaaaa gataataatc ttaacattcg tacctaaaga
    31921 aaaagaccgt agagtattaa cactacggtc ttttgattct attaaaaatt aaagtgatgc
    31981 ttgatcaacc gcaacaccag cacccattgt agtagagatg cttactttct tgatgaagat
    32041 accttttgct gttgttggtt ttgcttttgt taaagcagct aacaatgcgt ttaagttatc
    32101 ttttaattgt tcagctgaga agtctgcttt accgatagta gtgtggataa taccgttttt
    32161 gtcgttacgg tagcggatct gaccagattt cgcattttta accgcttctg caacgtttgg
    32221 tgttactgta cctactttcg ggtttggcat taagccgcgt ggacctaata cttgacctaa
    32281 ttgacctaca acacgcattg catctggaga agcgataaca acatcaaagt tcatttcgcc
    32341 tttcttgatt tgctctgcaa gatcttccat acccactaag tcagcacccg ctgcttttgc
    32401 tgcttctgcg tttgcacctt gtgtaaacac agcaacacga gcggtacggc ctgtaccgtg
    32461 tggtaatacg gttgcaccac gcacgttttg gtctgattta cgagaatcga tacctaagtt
    32521 tacagcaacg tccacgctct caacgaattt agcggttgcg aattttttta acacttcaac
    32581 tgcttcgttg atgtcataag ctttagtaga atctacgcca gctttaattg ctttcatttt
    32641 tttagtcaat ttagccattg tattattcct ctacgattaa gcccattgag cgagctgtac
    32701 cagcgattga tttcattttg gtttcgatag tcgcaccagt catatctgct gctttagttt
    32761 cagcgatttg acggatttgc tcttgagtta ctgtacccac tttatctttg ttcggtttac
    32821 cagaaccaga tttgatacct accgcttttt tcagtaacac tgccgccggt ggagttttgg
    32881 taacgaatgt gaaagattta tcagcgtaaa ctgtgataac aaccggaatc ggtaaacctt
    32941 tctctaagct ctcagtacga gcgttgaatg ctttacagaa ttccataatg ttaacacctt
    33001 gttgacctaa tgcaggacca accggtggtg atgggttagc catacccgct gcaacttgca
    33061 gtttaacgta ggcttggact ttttttgcca ttttaatttt cctctaaatg ggtgataacg
    33121 ctgaaaatca gctcccctgt tacataaaaa atacgagtca gcaggctggc tcgtaatagg
    33181 tcgcaaattt taaagaattt gcgactattt ttcaagcaaa actttcgctt tgttgaataa
    33241 atcttgttac aagcggtctt ttttccgctg aattttgcac aaaattgaga aaaataaccg
    33301 cttgtgtgtg aaattaattg aaattagctc tgcttttcca cttgactgaa ttctaattct
    33361 accggtgtcg cacgaccaaa aatcgacacc gatactttta agcgaccttt ttcgtaatcc
    33421 acttcttcga ccgtaccgga gaaatctgca aacggacctt ctgtaaccaa aatgctttcg
    33481 cccggttgga attcttttct gtggcgtggt ttttccgctg tttcttgtac acggtttaga
    33541 atgcggtctg cttcacgttt ggtaattggt gcggggcgat ctgccgtacc accaataaag
    33601 cccattacac gaggcacact tttcactaaa tgccaagtat cgtcattcat ttccatttca
    33661 accaatacat aacctgggaa gaatttacgc tcggttttac ggcgtttacc gcccacattt
    33721 tcaacgactt cttcggttgg cactaatact tcaccaaact gctcttccat tttgtgaagc
    33781 ttaatgtatt cacgcaatgt tactgccaca cggttttcaa agcctgaaaa cgcttgtaat
    33841 acataccagc gttttgagcc attttctttc acttctactt cactcatttt agaaccttaa
    33901 acttgttaca aatgtaatta atgcgacgat gattgagtca ataccccata aaaccaagga
    33961 aactactacg cacattgcca atacaatcaa ggtggtttgt gttgcttcag ggcgtgtcgg
    34021 ccaaataatt ttgcgtagtt cggttcgaga ctctttaata aaatgaattg ccttttgccc
    34081 ctgatttgta attgccgcta gtgcaacagc accaactagc aacaccacca ataaaagcac
    34141 tcgaacaatt aatgaaaaat gagatgaaaa ataggcatta cctaccgcag caactgctaa
    34201 aaatacaatc gcaaccagcc ataatgctgt atttgcacct ttgcttttcc cccctttttg
    34261 ttcgacttgc tcgggggttt ttgttttaat ctcagtagcc atatataacc taaaaaatga
    34321 ataaaaaaat cagaaattat tttaaagcgt taaattctat cattttctac aagacttgcc
    34381 acgatctttt atagattttt atgcaaaatc aatattatgc ggtgctatca cgaatcacta
    34441 gcttagtcgg taatttaaca atcatctctt ctgctgataa ggttttacca ctcaaaagag
    34501 taagtagttg tttacctgct atttttccca tttgctcgta agggatcgca atggttgtca
    34561 atttagggta ggaaatactc cccacttcta aaccacctaa accggcaatc gcaagatctt
    34621 ttggaatgct catatgtcgg cgtaatgctt catataatgc tccgcaagcc aattcatcgg
    34681 ataaaaaaac caatgcatct attgcttccc aatttagaag ggcttcagtc aacaaagttg
    34741 ctcctgccga aaacgaaata tcctctacta cgtggtggat ttttaaagga gaaatatgat
    34801 gtgcaaataa agtcgtatgc caactttcta aatattgttt gaagatggat aaatcttgct
    34861 tcgccgataa caatccaatc tctcgatagc cttttttcac caaaaaatgg atcattttct
    34921 ccattgcttc gtggctatca attccaacac tgagatgaat cggttctttt tttatcgcac
    34981 ctatttctac ggtagggata ttgctgtttt taatccactc acggacatta ggagggcaat
    35041 ctaaattcaa aaacactatc gcttgaggag catgattaat cagttcttga aaccaagatt
    35101 cttgatcagc gatcaaaatc accatttgca atgcggaatc cgccacgctt ttgcgtaatg
    35161 ccgataaaat taaggtattt tccgttgagc taatcgacgt cgtcacaatg accaaattat
    35221 gtgattctgc agaagctaat tgccgagcag aaaaatgcgg aatatatcct agttcaatga
    35281 ttgcctcttc aatccgttta cgtagtggct ccgacaccat ttctggtgta cgcaaagcac
    35341 gagataccgt catctgccca acaccaacaa ctttggctac atcagctaaa gtgactctgc
    35401 ccgaagatct tcgatttcta cttgttccat ttgccatact attttcctaa ttgttccata
    35461 ttatagggct aaatccttaa ttttcctatt tagaaagaac agagcaaaat gccaactgat
    35521 tattttttat ctaattaaat actaaaaatg ctaacgctat cacattttta atattttctg
    35581 tttgatagcg ttaacatttc tattctatag tatttctata gttcaaaatt cagacaacta
    35641 ttcattcacc tttaatggag atttactatg aaaattatgg cagtatgtgg ttccggctta
    35701 ggtagcagct ttatgatgga aatgaacatt aaaaaagtat tggcaaaact cggtgtcgaa
    35761 gcggaagtga gtcataccga cctttcctct gttgttgctg atgatgctga tttatttgtg
    35821 atggcaaaag atattgccgc aagcagcaat gtgccgaaag aaaaactcat cgtggtaaaa
    35881 aacattgttg gattagcaga attcgaagaa aaattgaccg cttactttaa ccaataacgg
    35941 agatgttata tggaatcgct actctttttt atattagata ttctcaaagt gccttccgtt
    36001 ctagtgggtt taattgctct ggtcggttta gttgcacaga aaaaatcggt atccgatatt
    36061 gttaaaggaa caatcaaaac tattttaggc ttcttagtgt taagcggtgg tgcaacagta
    36121 cttttaagct ccctgactcc tttaggcggt atgtttgaac acgccttcaa cgtgcaaggc
    36181 attatcccta ataacgaagc gattgtgtct atggcaatcg aaaaatatgg tacggcgact
    36241 gcgttaatta tggcattcgg tatggtagcc aatattttaa ttgcacgttt tacccgccta
    36301 aaattcattt tcttaaccgg acaccatacc ttctatatgg cgtgtatgat cggcgtaatc
    36361 ctaaccgttg ccggctttga aggtattcag ctcgtctttg tcggggcatt aaccttaggg
    36421 ttaattatgg catttttccc gactatcgct cacttctata tgaaaaaagt aaccggtagc
    36481 aatgatgttg ccttaggtca tttcggcaca ttaggctacg tgctcgctgg tgcaatcgga
    36541 caattagtag gaaaaggctc taaatctacc gaagaaatgg atttacctaa aaacctaggc
    36601 ttcttacgtg atagctccat ttctatttca ctcacgatga tggcgattta ctatattctc
    36661 gccattgctt caggcagtga atacgtttcc acattaagcg gtggacaaca ttatttagtg
    36721 tatgccacca ttcaagccat cacgtttgca gccggtgttt atgtcatttt gcaaggggtt
    36781 cgtttaattt tagctgagat cgtcccggca tttaccggct tctccgaaaa attagtacct
    36841 gatgccaaac cggctctcga ctgcccgatt gtgttccctt atgctcccaa tgcggtatta
    36901 gttggatttc ttgccagttt cttaggtggg attttaagct tagcgatttt aggacaactt
    36961 aattgggtgt taattttacc tggcgtagtg ccgcacttct tctgtggggc aactgccggc
    37021 gtatttggga acgccaccgg cgggcgtcgt ggtgcaatta tcggggcttt tattcacggt
    37081 atattaatta ctttcttacc ggtattctta ttacctgtac taggttcatt aggctttgct
    37141 aacaccactt tctctgactc agacttcggt ggtattggga ttgtgttagg ctatatggct
    37201 caatacttcg ataagaatat cattacgctc attattatcg gcttattcac tctgttggta
    37261 gcctataatt atttggcgaa aaaaccggca gagccgaaag cctaattaaa atcagatgtt
    37321 atttgctacc tgcgaggaat cttccctacc atttattgat ggtagggaat tttgtttagt
    37381 taaacgacaa cgctcttgtt caatacccat tatccatcac atacccccca ctcacttgtt
    37441 taattacgcc ggctaattcc agccccagta attcaactag caatgtctca atcgttaact
    37501 ctgtagctct ggctaaatcg tcaatcgcta taggctctaa gctgatatgc tgataaattt
    37561 gctgctggca agcggtcaga tttggcggat tttttgcaaa tttttcgcta cttttgaccg
    37621 cttgtaaaga tgtgcttact attggcttag gctcatcaaa caaagggatt tgttgaggtt
    37681 gatattgaga ggcgaccgcc tctaaaatat cttccacccc ctccacaagc aacgcccctt
    37741 ctttaattag cttgtggcaa ccttgtgcgt aagggttttg caccgcattc ggtaacgcaa
    37801 acacctcccg accttgttca agggcatagc gagcggtaat taaagagcca ctattaatgg
    37861 ttgcttcaat caccaaagtg ccgagcgata aaccgctgat tatgcggttt cggcgtggga
    37921 aattttcagc aatcggaggc tgattaggtg aaaattcaga caccaacgca ccacctgact
    37981 cgacaatttg ttcagccagt ttttgatggc gtgcggggta aatccggttt aagccactgc
    38041 ccaacacagc aatcgtaata ccttgatatt ccactacttt tttatgtgca aaaccgtcaa
    38101 tgccaatcgc taaaccgcta gtcaccgcca tattgtgctt caccaagtca gcggcaaatt
    38161 gccccgccca atattcaccg tagggcgaat aatcccgact cccaacaatc gcaatttgcg
    38221 gtaaggaaag gctttcgaca ttacccttta caaataaaat cggcggagcg gtggaaattt
    38281 gccgcagcaa gaaaggataa gctttatcaa ataaactcag gatttgctga ttcggctgct
    38341 ccgcccaagt tagtgcctgc tcaatccaat ttttatccgg attaaaccaa cgctgaatct
    38401 gcttttcatt ccagccgatc tgccttaact gtgttttatc gtaggcacaa aattcggcaa
    38461 aatccaactc tgccaataac cgtccgattc gctgcggacc aatttgcacc tgcaacaaac
    38521 gcaataaatc atactgatat gccatttttt cgccctcaaa atttccctaa taataagaaa
    38581 acgtttagaa tagacaaacg aattttttaa acttttcgag agagatcaca aaaatggcaa
    38641 tattagatgt tgtgctttac cccgatgaga aactggcaac cg
//
LOCUS       DANJWC010000004        37166 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.004, whole genome shotgun sequence.
ACCESSION   DANJWC010000004 DANJWC010000000
VERSION     DANJWC010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 37166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37166
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.004"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            88..1671
                     /locus_tag="PWX02_000120"
     CDS             88..1671
                     /locus_tag="PWX02_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1728181.1"
                     /translation="MKKQFEHNESRRGFMKLIAGVGAGMAFSGTLGTFTPKAFAAPAA
                     GSTIEAGIAYPISTGFDPLTSSGASSMAANLHIFEGLVDLHPATRKPYLALAAAEPEK
                     IDDVTYRITLRDGAKFHNGNPVTTEDVVYSFERVLDPAKASLFAQFIPFIDTVKKVDD
                     KVVEFKLKYPFALFKERLTIVKIVPKAVVEAGQAAFDANPVGTGPYKFVSATKDDRIV
                     FEAFADYNGGYPAQVEKMTWFLLSDDAARVTAQESGRVQAIESVPYLDAERLKRKGTV
                     ESVQSFGLLFLMFNCEKAPFNNPKVRQALHYGLDTQKLIDVVFLGNAKAATSYVQDTH
                     PDYVKANSQYDFDKAKAEALLAEAGVTELKFELLATDHSWVKECAPLILESWNSLKGV
                     KVSLKHLQSGALYGTHVDKGAYEVVIAPGDPSVFGNDLDLLLSWWYRGDVWPKRRFRW
                     SETAEYAEVQKLLDEAAKNPAASKEAWAKAINIIAEQVPLYPIIHRKLPTAWNDKALT
                     GFQPLPTTGMSFIGVGRAK"
     gene            1883..2836
                     /locus_tag="PWX02_000121"
     CDS             1883..2836
                     /locus_tag="PWX02_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1728182.1"
                     /translation="MEIILRLLWRRLMALPVMILGVTALVFIVLQFTPGDPATVALGE
                     SASEAAKEIYREQHGLNDPLLVQYFRFLGNLFVLDFGMTTPPEQPIVDMIAKAFPLTL
                     QLTFIGVFLAAIVSFTLGVTAALYRDTWIDQLIRLISVAAVATPSFWLGILLIQYFSL
                     KLDWLPSGGFIPFSEDPKGYVNSMVLPSLALAVPVCASLIRVVRTTMVEEMDKDYVRT
                     AIGNGVPYSTVIRHNVLRNALITPVTVLGLRVGYLLGGAVVIEQIFDLPGMGKLIFNG
                     IVNHDLNLVQGVVLTIAFTFVLVNIIVDILYLLINPKIRSL"
     gene            2838..4808
                     /locus_tag="PWX02_000122"
     CDS             2838..4808
                     /locus_tag="PWX02_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDL1728183.1"
                     /translation="MFRQGLAARLASGGARFRALSTSSKIALLFIVFIALVAIFAPLV
                     APYDPLQTLRPVQAPSGDYLFGTDRLGRDIFSRMVWGARTSLFIGLGAVGVAILFGSI
                     LGATAATADKFGNEVIMRFMDILMAFPGIALAAVLLATFGNSVPVIIVTIAVVYTPQL
                     ARVVRANVVSQWEEDYVRAERVIGGSRTYILLKHVVRNTAAPVLVFATVMVADAIVFE
                     ASLSFLGAGVQPPFPSWGNILSEGRNLVLSGFWWATTFAGIMILLTVLALNILAEGLT
                     DALVNPKLKRSTQSKEDVAKPLATDVQEAMSETLALKRYLLKLHEKETTRTDRMQLNS
                     TAKPILQVKNLSIRFPNRYGETPLVDNISFTVHEGETMGLVGESGCGKSITAFSIMGL
                     LPKTAKITGEILFTDRSGKQHNLLESADLNALRGHEISMIYQDALSALNPSMRIKDQM
                     AQLISRGGKQSAETLLQWVKLDPEKTLNRYPHELSGGQRQRVLIAMALAREPKLLIAD
                     EPTTALDVTVQAEVIKLLNELREKLGFAMVFVSHDLALVAQIAHHITVMYAGQVVETA
                     PTTPLLANPTHEYTRGLLGSVLSTELRAERLYQIPGSVPSPFDFAKGDRFASRSLRPD
                     ANPEQHLKLVPTGDHPQHFWASHLEEPQSKEA"
     gene            4823..5629
                     /locus_tag="PWX02_000123"
     CDS             4823..5629
                     /locus_tag="PWX02_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1728184.1"
                     /translation="MSMKFIKEQPIIELSNIVVQFASRDGTLFNPKKFTAVNDVSLAI
                     HAGETVGLVGQSGCGKSTLASVMIGLQKPTSGTVKFNGLEMKYGSAEARKYFGSQVSV
                     IFQDPATALNPRMRVLDILKDPMDIHNILQPQEREKRVYDLLSRVGLPRSAALVEPTR
                     LSGGQKQRVAIARALALNPKLIVADEPTSALDVSVRAQVLNLLADLKKELNLAMVFIS
                     HDLQTVHQVSDRIVVMNGGKIVEMGDASDVFERPTQEYTRTLIEAAPSLL"
     gene            5693..7279
                     /locus_tag="PWX02_000124"
     CDS             5693..7279
                     /locus_tag="PWX02_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDL1728185.1"
                     /translation="MIQITQTINIDLLIVGSGIAGLSAAVEAKEKGINTLLISKSTIG
                     SGASYFPLKATLGIQVTGDEADKIHFKEDIENIGKGKINPNVVQAYIEDSPQAIELLH
                     RIGFQPWKRNDNRPACFAKYPRPIYLIKDWRESALRAKQILQEQHIDFYENATLLHIV
                     TDNNQVQGAVFSLTTSGQISYIFCKTKHLILAAGGIAGLYKDNLYPADVIGSLHYVAQ
                     QAGATLVNLAFIQFIPAFVEPKYKVLFGEHTLKYVTQITDSKGNNLFPHLNAEQFAKM
                     VLERSHYAPFSLDFAGVEFDLVMMKHLLENPHEKGIYLKYSPKLYEDTEEFYTVYLNW
                     LKNEVGIDLVQDKIAIKPFAHSCNGGIQINQWGESAVKGLFAIGEISHCIEGANRMGG
                     NSVGGGLVFAKRAVSEIVRSLEQNDLQKNSEISPLAMAEKALNQLANSTANLELTASE
                     VLSTIREKMALYANVYRTRSNLKLLLEELNRLEQEFDPIYHAQYQGIEIYNALKTAQL
                     VVNQMLNEQSLGAHYLDEQN"
     gene            7325..8707
                     /gene="nhaC"
                     /locus_tag="PWX02_000125"
     CDS             7325..8707
                     /gene="nhaC"
                     /locus_tag="PWX02_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL1728186.1"
                     /translation="MNNYLLKLKPMTAVILSLLTVFILGFTMIGLGWVPQISILAVIV
                     MLLLFGLINKIPFKNMQEGMASGLMSGIGAVYLFFFIGLLVSALMMSGAIPTLMYYGL
                     SVISPELFYISAFALSSVVGIALGSSLTTTATIGVAFIGMASAFGANPAIVAGAVVSG
                     AFFGDKTSPLSDTTTIAASVVGIDMFAHIRNMLYTTIPAWILTALFLWFLTEPTDAQN
                     LANIENLKTNLQETGLIHGYSLLPFVTLILMAIKRVNSIYIIVTTVTLSLIITYIHSS
                     PTLEQLGNYFFTSYKPVEGVDLGEVASLVSRGGINSMFFTITIVLLALSLGGLLQILG
                     ILPALLNGISGFLTSTGRVTLVVASTAVGINVLIGEQYLSLLLSGNTFRSTYERLGLE
                     VKNLSRTIEDAGTVINPLVPWGVCGVFISSVLNVPVLDYLPYAFFCYICLILTLIFGF
                     TGVTISKKAN"
     gene            complement(8750..9940)
                     /gene="ispC"
                     /locus_tag="PWX02_000126"
     CDS             complement(8750..9940)
                     /gene="ispC"
                     /locus_tag="PWX02_000126"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDL1728187.1"
                     /translation="MKKLVILGSTGSIGKSTLSVVEHNPNQYEVFALVGGKNVELMLE
                     QCRQYQPTFAAMDDEKSAQALKTALATLGSKTEVLAGQAAICELAAHSEVDMVMAAIV
                     GAAGLLPTLSAVKAGKTVLLANKESLVTCGQIFIDEARKSGAKLLPVDSEHNAIFQSL
                     PPEAQQKVGFCPLAELGVSKIILTGSGGPFCTKPLNEFDAITPAQAVAHPNWSMGKKI
                     SVDSATMMNKGLEYIEARWLFNATAEEMDIIIHPQSIIHSMVRYIDGSVIAQMGNPDM
                     CTPIAHTMAYPNRINAGVPPLDFFKLKELTFIESDFARYPNLKLAIEAFAEGQYATTA
                     MNAANEIAVEAFLNEQIRFTDIVNVNRTVVENIAPIQVSEIADVLHIDKLARELAKQA
                     VLQL"
     gene            complement(10059..12257)
                     /gene="relA"
                     /locus_tag="PWX02_000127"
     CDS             complement(10059..12257)
                     /gene="relA"
                     /locus_tag="PWX02_000127"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HDL1728188.1"
                     /translation="MVAIRYSHQLEPNNFELSSWCASLNMSPVTFEQIHNAWSYAQEK
                     LDTDYSLMWYGIEMVEVLHGLNMDDDSLVAAMLFPLVKHNVIDLVQLKEDFSNDIKNL
                     VKGVLEMENIRQLSANNVSDLQIDNIRRMLLAMVDDFRCVVIKLAERIAYLRDSNHHS
                     EEDTVLAAKECSHVYAPLANRLGIGQLKWELEDYCFRILHPVSYTQIAKFELGERRVD
                     RERYIANFVADLTACLSPEIKNVQIYGRPKHIYSIWKKMQKKRLRFNQLFDIRAVRVI
                     VPTLEDCYTALGIIHTKYTHLPEHFDDYIANPKPNGYQSLHTVVLGEGDKTIEVQIRT
                     QQMHDDAELGVAAHWKYKEGATAGRSGYEEKIVWLRKLLEWQNDLADSGDMVAEMRSQ
                     IFDDRVYVFTPRGEVIDLPQKATPLDFAYAIHSEIGHRCIGAKVAGRIVPFTYHLQMG
                     DQVEIITQKEPNPSRDWLNPNTGFVNTGKARSKIIAWFKKLDREKNIPIGKEALEAEM
                     EKFGFTLKQIEEYALPRYNLKQFDDLYAGIGGGDIRLHQLMHFLQSKLIKPTAEQEDE
                     AVLKQVNKNTNQPKPSKGYVIVEGVGNLMHSIARCCQPIPGDEIVGYITQGRGISIHR
                     ADCEQLFELQSLNPERVVEADWGEGYTSGLSLTIRVIANDRNGLLRDVSAIMANEKVN
                     VLGVSSRTDVKRSLATIDMEIQLNNVEILNKLLARIAQLDDVIEAKRLSS"
     gene            12511..13818
                     /locus_tag="PWX02_000128"
     CDS             12511..13818
                     /locus_tag="PWX02_000128"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDL1728189.1"
                     /translation="MTILALGINHKTASVGLREKVAFVDDKRKLALEQIQTSGLAESV
                     VILSTCNRTELYFHQPNISPREESEENIQWREQCFRWFAQIHQLDESELRECLYFKQN
                     LEAANHLMKVASGLDSLILGEPQILGQVKQAYQYSEDFYQHQQKAMFTKLSRLFQRTF
                     ATAKRVRSETEIGSQAVSVAYAACGLAHQIFDNFGKLRFLLVGAGETIELVARYLIQH
                     GAKNIMLANRTHIRAEMLAEKLNAPMQILSLSALQLGLNQADVVISSTGSPDTLISKE
                     MVEIAQKQRQFDPLLLIDIAVPRDIDEKAGDLEGVYAYSVDDLQNIIQRNMAQREEAA
                     KQAAEIVKEESKAFFEWLKQQQSSDLIKRYRQDAEQTRQELLEKALQAISQGQDSQKV
                     LSELSYKLTNQLLHAPTQALQAMAKSGNSTGLQSFSRALRLEE"
     gene            14003..15538
                     /gene="der"
                     /locus_tag="PWX02_000129"
     CDS             14003..15538
                     /gene="der"
                     /locus_tag="PWX02_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDL1728190.1"
                     /translation="MTPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGH
                     ANIAGYDFIVIDTGGIDGTEEGVEEKMAEQSLLAIEEADVVLFLVDARAGLLPADVGI
                     AQYLRQREKTTVVVANKTDGIDADSHCAEFYQLGLGEVEQIAAAQGRGVTQLIEQVLA
                     PLGEQLNAEQFNQEQAGENAKNVANEETDEWDTEFDFENEEDAALLDEALAEEAEESI
                     EDKNIKIAIVGRPNVGKSTLTNRILGEERVVVYDMPGTTRDSIYIPMERDGQQYTIID
                     TAGVRKRGKVNLAVEKFSVIKTLQAIQDANVVLLTIDAREGISDQDLSLLGFILNAGR
                     SLVIVVNKWDGLSQDIKDQVKSELDRRLDFIDFARVHFISALHGSGVGNLFDSVKEAY
                     ACATQKTSTSMLTRILRMAADEYQPPLVNGRRVKLKYAHPGGYNPPIIVIHGNQVEKL
                     ADSYKRYLSNYFRKSLKIIGSPIRILFQEGNNPFAGKKNKLTPSQLRKRKRLMKFIKK
                     SKK"
     gene            15634..17061
                     /gene="hldE"
                     /locus_tag="PWX02_000130"
     CDS             15634..17061
                     /gene="hldE"
                     /locus_tag="PWX02_000130"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HDL1728191.1"
                     /translation="MMMQYSPKFNNAKVLVLGDVMLDRYWFGATNRISPEAPVPVVKV
                     QDIEERAGGAANVAMNIASLDVPVTLHGLIGDDDAGRALDKLLNSHQIQNHCVAINSH
                     PTITKLRILSRHQQLLRLDFEEGFHNVDSTALLAKLSQEITAYGALILSDYGKGTLES
                     VQQMIQIARNAKVPVLIDPKGTDFERYRGATLLTPNMSEFEAVVGHCKDEDEIVSKGL
                     KLIADYDLSALLVTRSEKGMTLLRPNHEPYHLPTQAKEVYDVTGAGDTVISVLATAIA
                     DGRAYEEACYLANAAAGVVVGKLGTSTVSPVELENAIHQRTATGFGLVSEAELKAIVE
                     EAKKRGEKIVMTNGCFDILHPGHVSYLENARKLGDRLIVAVNTDASVKRLKGEARPIN
                     DLAARMAVLAGLASVDWVVPFDEDTPQRLISEILPDLLVKGGDYKPEEIAGSQEVWAN
                     GGDVRVLNFENGCSTTNVIKKIQAL"
     gene            17075..17629
                     /gene="pyrR"
                     /locus_tag="PWX02_000131"
     CDS             17075..17629
                     /gene="pyrR"
                     /locus_tag="PWX02_000131"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDL1728192.1"
                     /translation="MTREKIIIDTEQFQRTISRISHQIIEKHGDLQDVVIVGIKRRGA
                     EIAELIQKRIEDLAQTRLPYMALDITFYRDDLDLIYQDPVFMGADNQLNLNGKKVILV
                     DDVLFTGRTIRAALDALLDFGRAARIELVIFVDRGHRELPIRADYVGKNIPTAKNEQI
                     QVQTLSYDGINQVVLVPAANKESA"
     gene            17702..18655
                     /locus_tag="PWX02_000132"
     CDS             17702..18655
                     /locus_tag="PWX02_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL1728193.1"
                     /translation="MKMKLVKSLFAVAVATMSLTQTAHAFEERVVALVNDTPIMQSQV
                     QRVLGKKKATETAQRAAIDQVIDDMLVQQAMKEAGVKVSPAAVNQAVENVAIQNGITY
                     GQLLDALDYQGITLEQFKRNIAQQMAMEQVRHISISKSIQVAPQQVQALAKELMAKDK
                     AAGKLKTVSGKEHRISHILLKTNPILNDAQAKAKLSSLTADINSGKISFEEAAKAHSV
                     DYASGTDGGDLGFNFLEIYDPAFANVASKIKPNQISAPFKSQFGWHILKVTDTRNGDR
                     TEDAYHQRAYQQLVNKQAEEASKDWVKALRKTANIQLSVNN"
     gene            18713..19579
                     /gene="rsmA"
                     /locus_tag="PWX02_000133"
     CDS             18713..19579
                     /gene="rsmA"
                     /locus_tag="PWX02_000133"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDL1728194.1"
                     /translation="MSSTNSKKHLGHTARKRFGQNFLHDMNIIHSIVSAINPKNGQFL
                     LEIGPGLGALTEPVAELVDKLTVVEIDRDLAERLRHHPFLNQKLTIIEQDALRFNVRD
                     YFNQLNLDEDSVRVFGNLPYNISTPLIFHLLTFHDLIQDMHFMLQKEVVKRLCAAPNS
                     KAYGRLTIMAQYYCQVIPVLEVPPTAFKPAPKVDSAVVRLVPYNQLPYPAKDVYWLNR
                     VTTQAFNQRRKTLRNALSTLFSAEQLEALGVDLTARAENLTIADYVRLANWLHDNPPA
                     IDTTEELVDEDC"
     gene            complement(19690..20319)
                     /gene="upp"
                     /locus_tag="PWX02_000134"
     CDS             complement(19690..20319)
                     /gene="upp"
                     /locus_tag="PWX02_000134"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDL1728195.1"
                     /translation="MKIVEVKHPLVKHKLGLMREAEISTKDFRELANEIGSLLTYEAT
                     KDLEMESVEINGWSGEKIAVERIKGKKVTVVPILRAGLGMMDGVLEHIPSARISVVGI
                     YRDEETLKPVPYFSKLANDVDERLAIITDPMLATGGSMVATIDLLKKAGCKQIKVLVL
                     VAAPEGIKVLEAAHPDVELYTASIDSHLNENGYIIPGLGDAGDKIFGTK"
     gene            complement(20344..22029)
                     /gene="fadD"
                     /locus_tag="PWX02_000135"
     CDS             complement(20344..22029)
                     /gene="fadD"
                     /locus_tag="PWX02_000135"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HDL1728196.1"
                     /translation="MEKIWLNNYPPYSPKTIETDQYESLVEMFEAAIQRHPDIPAYIN
                     MGQVLTFRKLEERSRSFAAYLQNELRLKKGDRIALMMPNLLQYPIALFGALRAGLVVV
                     NVNPLYTPRELEHQLNDSGAKAIVIVSNFATTLEKIVFNTEIKHVILTRMGDQLSFGK
                     RTLVNFVVKYIKKLVPKYKLPHAVSFREALSIGKQRQYVKPNIVSQDLAFLQYTGGTT
                     GVAKGAMLTHQNLIANMMQAKWIVEPLLGNSANMIGLVPLPLYHVLALSMNCLLFIEL
                     GLTGLLITNPRDIPGFIKELKKYKVSVITGVNTLFNALLNHESFKEVDFSHLKLSVGG
                     GAAIQSSVAKRWHDLTGVHIIEGYGMTECSPLISATRGDSVEYSGSIGVPVPNTDIRI
                     VDDEGKEVPLGERGELVVKGPQVMQGYWNRPDETAQVLQDGWMATGDIVVMGDDLNLR
                     IVDRKKDMIIVSGFNVYPTEIEEVISQNPKVNEVVAIGVPSEASGESIKIFVTKKDES
                     LTRDELRTYCRQFLTGYKIPRDIEFRDELPKSNVGKILRRVLRDEELAKVNKA"
     gene            complement(22007..22471)
                     /locus_tag="PWX02_000136"
     CDS             complement(22007..22471)
                     /locus_tag="PWX02_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HDL1728197.1"
                     /translation="MMIEQATVVEYQNGVAIVQCYAKSSCGGCAAQSACGSKALSALA
                     GEKIDPRFRIEVDEPLKVGDKVELGLAENILLKSVFLIYGIPLLVLVMTAVGFSQFFA
                     NELIVLFIMLLSTGITFWAVRKLIHKHSKQANFSPIFLGKVLGDYNGKNMAK"
     gene            complement(22468..23451)
                     /locus_tag="PWX02_000137"
     CDS             complement(22468..23451)
                     /locus_tag="PWX02_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HDL1728198.1"
                     /translation="MLFKIKKYLLLFWVISACYTFAWAESTIKTPLAYLTAMSEAHKK
                     STYELVYILQQGSDTESFRLRHAFTHNKEYAQLLNLDHSREEIILKNNKVSYLGYNFR
                     PFSLNSSQILDNLPNVLYTDYANLKGYAFLDAGKDRISDRITKVIRMKPNDTLRYAYI
                     LWIDEETHLLLKSQVLNMDNLVLEEFRVLQLYQSPELDRIANVIESLMLPTLTTVKKE
                     EFPKSDGWQVKWLPTGFRLIQQQTVTGATYQVESEYVDSRVYSDGLSTLTIYIMPSQG
                     VSFNEYAWQQGKLTILNQTINDRDIVIIGDVPLQSAKQIMNNIGFKEGAQP"
     gene            complement(23609..24232)
                     /locus_tag="PWX02_000138"
     CDS             complement(23609..24232)
                     /locus_tag="PWX02_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL1728199.1"
                     /translation="MQERITQQNERLSAYMDGQNVDTAFVETLTNSPELQQKWASYHT
                     IRSVMQGDEIILGTDFSAKMEALLENEEIESQANVEKSKGLLLKLKRWGTPIMQAGIA
                     ASVCLVAVLGVNSMNASDEMAQTQQPVLQTLPFSNSVEAVSYNAPVQDQPTEEQLELQ
                     QRKINALLENHELQRRTNNVKNLTLSEEEKQKAQTSSNTESEPNIQK"
     gene            complement(24278..24850)
                     /gene="rpoE"
                     /locus_tag="PWX02_000139"
     CDS             complement(24278..24850)
                     /gene="rpoE"
                     /locus_tag="PWX02_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HDL1728200.1"
                     /translation="MSEQITDQELVEKAQKGDKKAFNLLVVRYQNRVAGLLTRYVARD
                     DIPDIVQESFIKAYRSLESFRGESAFYTWLYRIAVNTAKNHLTSLGRRPPKEDILAED
                     AESYDVGAQLRESDTPENLILSEELKKVVFDTIENLPDELKTAIRLRELEGLSYEEIA
                     EVMECPVGTVRSRIFRAREAIDAKIKPLMQ"
     gene            complement(25072..26616)
                     /gene="leuA"
                     /locus_tag="PWX02_000140"
     CDS             complement(25072..26616)
                     /gene="leuA"
                     /locus_tag="PWX02_000140"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDL1728201.1"
                     /translation="MSNNNIIIFDTTLRDGEQSLKASLTVKEKLQIALALERLGVDVM
                     EVGFPVSSAGDFESVQTIARHIKNSRVAALSRAVAKDIDAAYEALKVAEAFRIHTFIA
                     SSALHVEAKLKRSFDDVVEMAVAAVKRARNYTDDVEFSCEDAGRTGIDNICRIVEAAI
                     NAGATTVNIPDTVGFCLPNEYGNIIHQVMNRVPNIDKAIVSVHCHNDLGMATANSLTA
                     VQNGARQIECTINGIGERAGNTALEEVVMAIKTRQEFMGVDTRINTQEIHRVSQMVSQ
                     LCNMPIQPNKAIVGSNAFAHSSGIHQDGMLKNKNTYEIMSPETIGLKKEKLNLTARSG
                     RAAVKGHMADMGYTETDYDLDKLYEAFLQLADKKGQVFDYDLEALAFIDMQQGDEDRL
                     KLDVITTQLISGLPASAFVQVELDGVRQSATSQGGNGPVEATYNAIMNIVGMELKMSH
                     YNLTAKGEGAEALGQVDIVVEYEGRKFHGVGLATDIVESSARALIHAINAIYRSQKVA
                     DLKAKK"
     gene            27100..27915
                     /locus_tag="PWX02_000141"
     CDS             27100..27915
                     /locus_tag="PWX02_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL1728202.1"
                     /translation="MTLPFRAIISDLDGTLLNANHKIGDFTIDTLEQLSKKGVDIFFA
                     TGRNHPDVSQIIGKVNVKNAMLVTSNGARANNLEGKKILNHYLPEDLAFELMNIEFDD
                     QNVCLNSYQGDEWFINKEIAELKKFHQDSGFSYQVVNFAHHHGRNTEKVFFIGKTAEA
                     LVPIEHYIRDTYGDRVQMTYSALLCLEVMAKGVCKANALAELVKLRGYTLADCIAFGD
                     GMNDVEMLSLAGKGCLMGNADPRLKAVLPNNEVIGFNKDEAVASYLRATFGIV"
     gene            27981..29144
                     /locus_tag="PWX02_000142"
     CDS             27981..29144
                     /locus_tag="PWX02_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDL1728203.1"
                     /translation="MKRKTLATLVLIAALGIGGYFYYQQQTNKPQIHYITQAVTRAEI
                     NKNVLATGSVRANKRTEVGAQVSGKIQKLYVELGQSVKQGDLIADIDSSNQSNTLSTA
                     QARLSSYKAQLTSAQVALEVAQSNYNRLNKLYRANSASLNDLETAKNTLASAKATVDN
                     IKAQLKSAEISVNDAKTNLTYTQIISPMDGVIVSVPVSVGQTVNSNQTSPTIVQVADL
                     SKMLIKLEISEGDIAQVKVGQKVKFSTLAEPNREYSATIDSVDPALTTLTDNNYTEQS
                     GNTEAVYYYANVSVDNADNSLRIGMTTQAQITIDKREQVLVVPTSVIKKRGKENYVLV
                     LENEQSVERKIQIGISDTQNTEVLAGLNEGDQVIVTQRADDEKISTARGPRMF"
     gene            complement(29257..30201)
                     /locus_tag="PWX02_000143"
     CDS             complement(29257..30201)
                     /locus_tag="PWX02_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HDL1728204.1"
                     /translation="MQTLLKFTQFISKTFAIWVVFFAFIAAQFPETFKQFLPWIPYLL
                     GIVMLGMGLTLTFKDFAEVTKNPKSVIIGVIAQFVVMPSVAYGLAKAFNLPADLAIGV
                     ILVGCCPGGTSSNVMTYLARGNTALSVACTTISTLLAPVLTPAIFYLFASQWLEINAT
                     AMFISVLQMVLLPIFVGVVIRTIFKQKVEQFSQTMPLISVIAIVLIVTAVVSVSRDRI
                     IESGLLIFAVVALHNGLGYLIGYLASRLLKLPIADSKAVAIEVGMQNSGLGAALAALH
                     FKANPIIAVPSAVFSFWHNISGPILAMIFAKMKNDNEK"
     gene            30349..31170
                     /locus_tag="PWX02_000144"
     CDS             30349..31170
                     /locus_tag="PWX02_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010869033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HDL1728205.1"
                     /translation="MKNIKLSAMSLTALFVLTACSHTHKNSDEHHQMVLQEQATLGIN
                     WMQQSGEYQALAHQAFNAAKVAFDQAKVKKGKKKAVVVDLDETMVDNSLYAGWKVKNH
                     KPFDGESWTRWVNARQTGAIAGAVEFNNYVNSHKGTMFYVSNRKDSSEKAGTIDDMNK
                     LGFSGVSEQTLLLKKDKSNKTPRFEEIEKQGYEIVLYIGDNLNDFGDATYRKSNAERR
                     AFVAENSQQFGKKFIMLPNPNYGDWEGGLDKDYYKGNAESRVKIRHNAIKAWDGK"
     gene            31309..32589
                     /gene="pepB"
                     /locus_tag="PWX02_000145"
     CDS             31309..32589
                     /gene="pepB"
                     /locus_tag="PWX02_000145"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HDL1728206.1"
                     /translation="MTAMKISLSTQNAPEIWGKNAILSANDAGFTVHNPNNDLATIQK
                     AARKIKNQGISTVELTGDSWDLKSCWAFHQGFHSVKNQGSVQYPDLDEQQAEFDNRVK
                     CATFVRELINKPSDKLTPEKLAEQAVEFIQKMAKPNTVSYQIIRGEALQEQGYHGIWT
                     VGKGSANPPAFLVLDFNPTNDENAQVLACLVGKGITFDTGGYSLKPSDSMSTMRTDMG
                     GAALLTGSLAFAIARGLNKRVKLYLCCAENMVSHNAFKLGDIITYRNGVTVEVLNTDA
                     EGRLVLADGLIDACKQNPQFIIDAATLTGAAKVAVGNDYHSVLSMDDNLTEALFKSAK
                     TENEPFWRLPFEEFHRGQIASSFANIANIGSVPVGAGASTATAFLSYFVENYQQNWLH
                     IDASATFRKTASDLWAAGATGLGIRTVANLLLSK"
     gene            32640..33122
                     /gene="greB"
                     /locus_tag="PWX02_000146"
     CDS             32640..33122
                     /gene="greB"
                     /locus_tag="PWX02_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HDL1728207.1"
                     /translation="MAKSNYITRSGWQALDQELKFLWREERPKVTQAVSDAAALGDRS
                     ENAEYIYGKRRLREIDRRVRFLSKRLEVLQIVDYHPKQEGKVFFGAWVELENEEGEAK
                     QYRIVGCDEFDPAKNWISIDSPVARALIGKQVDDEIRVETPLGKVLLYINKIWYEQAS
                     "
     gene            complement(33164..36688)
                     /gene="recB"
                     /locus_tag="PWX02_000147"
     CDS             complement(33164..36688)
                     /gene="recB"
                     /locus_tag="PWX02_000147"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HDL1728208.1"
                     /translation="MKNLNPIQLPLNKSVLIEASAGTGKTFTIANLYLRLLLGIGCNP
                     LTVEQILVVTFTKAATEELKNRIRKNIQQCADFLKDQADGLEVESTKSYRNNLDFLAQ
                     IYPLIPNIHEALLRLSIAEREIDTASVFTIHGFCQKMLVQFAFESGVRFDLDLQPNQS
                     DLLKKLSEEVWREQFYPQDLAITYAVAEQLGTPEYALNAVRRYLSTELPEPNASLNQD
                     IAEHLAQYQQFINEVKSYWKTHSDELVETIRHDLKHKITLHGGWYRATTFEAGVEVMN
                     QWANSQHNHFPAGLYLFTTEKIAAGTKKSCEPIDSPHLAKNQAFLTAYKERFENSLSA
                     MLQYQFLIALKAKLAEYKQSHPQRSFDDLLTMLNQALKNLHTGEALAQKIRAMYPFAM
                     IDEFQDTDLTQYEIFSQIFMAENAENGFIMIGDPKQSIYKFRGADIFTYLRAAKQADE
                     QFTLGKNWRSLPEVVQAVNNLFSFANDNSPFLYEDIGFHPVEADSQKGQLVSEQGHFV
                     GYITENSKLDDLAEMCAYQIHQQLEAMAQGKFGVEKNGEFQSFAAKDIAILVRKGSQA
                     KRIKKALAKRNIKSVYLAESASVFGSEIATELCWLLQAALNPYHYKALLSALGSTLWG
                     LTAAEIHRYKENEQLWDLKVNQFVEYSNIWKEQGVLPMLHKIYLEQGIIERLKGLENA
                     DRLITDLLHLTEILQETSQALESEAELLRWYEKQVNSPDFNDEQQLRLESEEELIKIV
                     TIHGSKGLQYPIVWLPFVSQKSEGDTQTALSLYRNSQGALDWCLGSEEASFSLQYEAY
                     AEDLRLLYVALTRAESQLNLVLPKEISGWSPLHYLLADGEIPTGKVPVSDYFSQKRIA
                     ANLVEVAGELPFSLWEGEQSSDDYQAKTFSGNIRVVGQITSFTSLYAQHERFSEGKSE
                     RVMDLAQDRDVHIETIEAGYSSETAAFSPFQFPHGIKVGTVLHHFFEHCQFNEQIDRE
                     AVAKVCEQLGLSEEWIEPTALWFERILTTPLAEANFCLKAIDETKRLNEWQFYLRLKN
                     DKALHQLNALLKQHSPLAKTLPELQLPQLEGFVRGFVDCIVQVEEKFYLIDYKSNFLG
                     YLPQNYAKEHIQREMGRQRYDLQYLLYTLALHRYLTARLGEKYDYERDFGGVAYLFLR
                     AMNGENDNGVYFDKPTKQLIEEMDRLFG"
     gene            complement(36747..>37166)
                     /locus_tag="PWX02_000148"
     CDS             complement(36747..>37166)
                     /locus_tag="PWX02_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDL1728209.1"
                     /translation="TATALAIIAAIIIILLKNFIPFAFTNNPISIAITAHLLIFAAVY
                     QIPDAIQAVANGILRGYKHTQPILYVTLFCYWGVSMPLGYILARTDWIVQPMAAQGFW
                     LVFCIGLSLAGGLLLWQMRKIQAVPKAQLIAKLERIK"
BASE COUNT        10967 a         7956 c         7392 g        10851 t
ORIGIN      
        1 attcaattta aaaaaagtgc attttttgat aaattcactt cagcaaataa taccgcccct
       61 atcataaaaa cttaaaggag ctttaagatg aaaaaacagt ttgaacacaa cgaatcccgc
      121 cgtggtttta tgaaactaat tgcaggtgtc ggtgccggta tggcatttag tggcacttta
      181 ggtacattta cacctaaagc atttgcagcc cctgccgccg gctcaacgat tgaggccggt
      241 atagcttatc ccatatcaac cggatttgac ccgcttacct caagcggtgc atcctcaatg
      301 gcggcaaacc tccatatttt tgaaggcctc gtggacttac atccagccac tcgcaaacca
      361 tatttagcat tggcagcagc agagcctgaa aaaattgatg atgtaaccta tcgaattact
      421 ttacgtgacg gggctaaatt ccacaatggt aatccggtta ccactgaaga cgtagtttac
      481 tcatttgaac gtgtattaga cccagctaaa gcatcacttt tcgcacaatt cattccattt
      541 attgacactg tgaaaaaagt tgatgacaaa gtggttgaat tcaaattgaa atatccattt
      601 gcgttattca aagagcgcct aaccattgtt aaaatcgtac caaaagcagt ggtagaagcc
      661 ggtcaagctg catttgatgc taatccagtg gggactggcc catataaatt tgtatccgca
      721 actaaagatg atcgtattgt tttcgaagca tttgcagatt ataacggtgg ctacccggca
      781 caagtcgaga aaatgacttg gttcttactt tctgatgatg ccgctcgtgt aaccgctcaa
      841 gaatctggcc gtgttcaagc aattgagtct gtgccttact tagatgcaga acgcttaaaa
      901 cgtaaaggta ccgttgagtc agtacaatca ttcggcttac tgttcttaat gtttaactgt
      961 gaaaaagcac cgtttaacaa cccgaaagtt cgccaagcgt tacattatgg cttagatacc
     1021 caaaaattaa ttgatgtggt gttcttaggc aatgcaaaag ctgctacatc ttacgtgcaa
     1081 gatactcacc cggattatgt gaaagcaaat tcgcaatatg atttcgataa agcgaaagcc
     1141 gaagcgttat tagcagaagc gggtgtgaca gagttaaaat ttgaattgtt ggcaaccgat
     1201 cactcttggg tgaaagaatg tgcgccatta attcttgaat cgtggaacag cttaaaaggc
     1261 gtgaaagttt cactcaaaca cttacaatca ggtgcattat atggcacaca cgttgataaa
     1321 ggtgcatacg aagttgtgat tgcacctggc gacccatcag ttttcggtaa tgacttagac
     1381 ctattattaa gctggtggta tcgtggtgat gtatggccga aacgtcgttt ccgctggtct
     1441 gaaacagctg aatatgcaga agtacaaaaa ctgcttgatg aagcggccaa aaatcctgct
     1501 gcttctaaag aagcctgggc gaaagcaatc aatattatcg ctgaacaagt gcctctttac
     1561 ccaattattc accgtaaatt gccaactgcg tggaatgata aggcattaac aggtttccaa
     1621 ccattaccaa caacaggtat gtcatttatc ggtgttggtc gtgctaaata gtcgtaacgg
     1681 ctagctaaat gcaagcggta atattttaca aaaaatttac cgcttgcata aataaaaatc
     1741 aaaatgatgt aatattcgtc ttatatcact cagaattctt accatttcta tattttcatt
     1801 tataatccta catatcacta tattagcttg taggtaaact tagatttaat tttggagtgt
     1861 aactacactc aaggagaaga aaatggaaat tatactccga ttattatggc gtcgcctaat
     1921 ggctctgccg gtaatgatct taggcgtaac tgccttggta tttattgttt tacaattcac
     1981 ccccggagat ccggcaaccg ttgctctcgg tgaaagcgcc agcgaagccg ctaaagaaat
     2041 ctatcgtgaa caacatgggt taaatgatcc attgcttgta caatattttc gctttttagg
     2101 gaatttattc gtattagatt tcggtatgac aacccctcca gaacagccta ttgttgatat
     2161 gattgctaaa gcattccctt taacattaca gcttaccttt attggggtct tcttagctgc
     2221 tatcgtctct ttcacattag gtgtaacggc agcactttac cgtgatactt ggattgatca
     2281 attaatccgt ttaatctcgg ttgccgctgt tgccacacct tctttctggt tgggtatttt
     2341 attgattcaa tacttctcct taaaattaga ttggttgcct tcaggcggtt ttattccatt
     2401 tagcgaagat ccaaaaggct atgtaaactc aatggttctc ccttcactcg ctttagctgt
     2461 tcctgtatgt gcctcattga tccgtgtcgt gcgtacaaca atggtagaag aaatggataa
     2521 agattatgtt cgaacagcca tcggtaatgg tgtaccttac tcaacggtga ttcgccacaa
     2581 cgtgcttcgt aatgccttaa ttactccggt aacagtatta ggtttacggg ttggttattt
     2641 attaggtggt gcagttgtga ttgaacagat cttcgactta ccgggaatgg gtaaattaat
     2701 tttcaacggt atcgtcaatc acgacttgaa cttagtgcaa ggtgtagtat taactatcgc
     2761 atttactttc gtgttggtga atatcattgt tgatattctt tacttgctca ttaatccaaa
     2821 aattcggagt ttataatatg tttcgccaag gattagctgc tcgacttgct tcaggcggtg
     2881 caagatttag agcattatca accagctcaa aaatcgcctt actctttatt gtatttatcg
     2941 cattagtcgc tatttttgca ccattagtag cgccttatga cccactacaa acgctacgtc
     3001 cagtgcaagc tccaagcggt gattatttat tcggtacaga ccgtttagga cgtgatattt
     3061 tctcgcgtat ggtatgggga gcaagaacat ctctctttat cggtttaggt gcggtaggcg
     3121 ttgcgattct attcggtagt attttagggg caaccgcggc aaccgccgat aaattcggta
     3181 acgaagtgat tatgcgtttt atggatatct taatggcatt cccgggtatc gcattagctg
     3241 ctgtattact tgccactttc ggtaactctg tacctgtgat tatcgtgact attgcggttg
     3301 tttacactcc ccaacttgcc cgagtggtgc gtgctaacgt agtatctcaa tgggaagaag
     3361 attatgtgcg tgccgagcgt gtaattggtg gtagccgtac ttatattcta ttaaaacacg
     3421 ttgtgcgtaa tactgctgca ccggttttag tattcgcaac agtgatggtt gctgatgcca
     3481 tcgtatttga agcatcactt tcattcttag gtgcaggtgt ccagcctcca ttcccatctt
     3541 ggggaaatat cttatctgaa ggtcgtaacc ttgtattaag tggcttctgg tgggctacaa
     3601 ctttcgcagg gattatgatt ttattaaccg tacttgcgtt aaatatcctt gctgaaggct
     3661 taaccgatgc gttagtaaat ccaaaactaa aacgttcaac tcaaagtaaa gaagatgtcg
     3721 caaaaccatt ggcaacagac gttcaagaag caatgtctga aactcttgcg ttaaaacgtt
     3781 acttactcaa attacacgag aaagaaacga cccgtactga ccgtatgcaa ttaaactcaa
     3841 cggccaaacc gattttacaa gtgaaaaact tatcaatccg tttcccaaat cgttatggtg
     3901 aaactccgct tgttgataat atcagcttta ctgttcacga aggcgaaacc atgggcttag
     3961 tgggtgaatc tggttgtggt aaatcaatca ccgcattctc aattatgggt ttattaccaa
     4021 aaactgcaaa aatcactggt gaaatcctct ttaccgaccg ttcaggtaaa caacacaatc
     4081 ttttagaatc agccgattta aatgcccttc gtggtcacga aatctcgatg atttaccaag
     4141 atgcattaag tgcgttaaac ccatcaatgc gtattaaaga ccaaatggca caattaatca
     4201 gccgtggtgg taaacaatca gcagaaacat tattgcaatg ggtaaaactt gacccggaaa
     4261 aaacgctcaa ccgttatcct cacgagttat ctggaggaca acgtcagcgt gtattaattg
     4321 caatggcact tgctcgtgaa ccgaaactat tgattgctga tgaaccaaca acagcattag
     4381 acgtaaccgt tcaagctgaa gtgatcaaat tattaaacga attacgtgag aaacttggat
     4441 ttgcaatggt attcgtgagc cacgacttag ctttagtggc acaaatcgct caccacatca
     4501 cggtaatgta tgctggtcaa gtggttgaaa ctgcaccaac tacgccatta ttggcaaacc
     4561 caacacacga atacacacgt ggattattag gctcggtact ttctaccgaa ttgcgtgcag
     4621 aacgcctata ccaaattcca ggcagtgtgc cttcaccatt tgattttgcg aaaggtgacc
     4681 gttttgcgag tcgttcatta agaccagatg caaaccctga acagcacttg aaattagtgc
     4741 caaccggtga tcacccacaa cacttctggg cttcacactt agaagaacca caatctaaag
     4801 aagcataaag gagagcaata cgatgagtat gaaatttatc aaagaacagc caattattga
     4861 actgtctaat attgttgtgc aatttgcttc tcgtgacggc accttattta acccgaaaaa
     4921 attcacggcg gtaaatgatg tcagcctagc aattcacgcc ggtgaaacag tcggtctggt
     4981 tggacaatct ggttgtggca aatctacttt agccagtgtg atgatcgggc ttcaaaaacc
     5041 aacctccggc acagtaaaat ttaacggctt agagatgaaa tatggcagtg cggaagctcg
     5101 caaatatttc ggtagccaag tgtcagtaat tttccaagac ccggcaaccg ccctaaatcc
     5161 gcgtatgcgt gtgttggata ttttaaaaga tccgatggat attcataata tcttacaacc
     5221 gcaagaacgt gagaagcggg tttatgattt gctctctcga gtcggtctac ctcgttcagc
     5281 ggcgttggtc gagccaaccc gattatctgg tgggcaaaag caacgtgtag cgattgctcg
     5341 tgcattggca ttaaatccga agctgattgt ggctgatgaa ccaacctctg cacttgacgt
     5401 atcggttcga gctcaagtac tgaacctgct ggcagacttg aaaaaagaac tcaatttagc
     5461 gatggtattt atctctcacg acttacaaac cgttcaccaa gtttcagacc gcattgtggt
     5521 tatgaacggc ggtaaaattg tggaaatggg cgatgcttca gacgtatttg aacgcccaac
     5581 gcaagagtac acccgaacct taattgaagc agcaccatct ttattataat tgtgctttgt
     5641 gcctacaagc ggtaaaattt ttcgcaaatt ttacccgctt gtgtttttat ttatgatcca
     5701 aattacccaa accattaaca ttgatttatt aattgtcggc tctggcatcg ctgggctttc
     5761 tgctgcagtt gaggcaaaag aaaaaggcat taatacctta ctgattagca aatctacgat
     5821 tggctcggga gcgagctact ttccgcttaa ggcaacactt ggcattcaag taaccggcga
     5881 tgaagccgat aaaattcact ttaaagaaga tattgaaaac atcggcaaag gcaaaatcaa
     5941 ccctaatgtg gtacaagcct atatcgaaga ttcaccacaa gcgattgaac tactccaccg
     6001 tatcggcttt cagccttgga agcgtaacga caaccgtccg gcttgttttg ccaaataccc
     6061 tcgcccaatt tatttaatta aagattggcg ggaatctgcc ctacgagcga aacagatttt
     6121 gcaagagcaa cacattgatt tctatgaaaa tgctaccctt ttacacatcg tcacagacaa
     6181 taaccaagtg caaggtgcgg tattttcgct cactacaagc ggtcaaattt cctatatttt
     6241 ttgcaaaacc aagcatctta ttttagccgc aggcgggatt gctgggctgt ataaagataa
     6301 tctctacccg gctgatgtta tcggttcact tcattatgtg gcacaacaag ccggggcaac
     6361 cttggttaat cttgccttta ttcagttcat tcctgccttt gtcgagccaa aatacaaggt
     6421 gctatttggc gaacatacat tgaagtatgt cacccaaatc accgattcta agggtaacaa
     6481 tctatttcca cacttaaatg ccgagcaatt tgcaaaaatg gtgctggaac gtagccatta
     6541 tgctcctttc agccttgatt ttgccggcgt ggagttcgat ttagtgatga tgaaacattt
     6601 gcttgaaaac cctcacgaaa aaggcattta ccttaaatac agccccaaat tgtatgaaga
     6661 cacggaagag ttttataccg tctatttgaa ttggctcaaa aatgaagtcg ggattgattt
     6721 agtgcaagat aagattgcaa tcaagccgtt tgctcatagc tgtaacggcg gcattcaaat
     6781 taatcaatgg ggcgaaagtg cagtaaaagg gctatttgcg attggtgaga tttctcactg
     6841 cattgaaggg gcaaaccgta tgggtggtaa ctcagtcggc ggtggcttag tgtttgccaa
     6901 acgtgcggta tcggaaattg ttcgttcgct agaacagaat gatttgcaaa aaaacagcga
     6961 aatctcaccg cttgcaatgg cagaaaaagc attaaatcag cttgctaatt caactgctaa
     7021 tctagaacta actgccagtg aagtgctttc tactattcga gagaaaatgg cgttatacgc
     7081 caatgtgtac cgcactcgga gcaacctcaa actactactt gaggaattaa accgtttgga
     7141 acaagaattt gacccaatct atcacgctca atatcaaggc attgaaattt acaatgcgtt
     7201 aaaaaccgct caattagtgg tcaatcaaat gctaaatgag cagagtttag gcgcgcacta
     7261 cttagatgaa caaaattagt ctataatagc caacttattt aaaaataata aattaggagt
     7321 ataaatgaat aactacctac ttaaattaaa accaatgaca gcggtcatac tttcgctttt
     7381 gacggtcttt atcttaggtt ttacaatgat tgggcttggc tgggtgcctc aaatttcaat
     7441 tctagccgtg attgtaatgc tgctactttt cggcttaatc aataaaattc cgtttaaaaa
     7501 tatgcaagag ggtatggcaa gcgggctgat gtccggtatt ggtgcggttt acctattctt
     7561 ttttattgga ttattggttt cagctttgat gatgtctggt gcgattccaa ccttaatgta
     7621 ttatggctta agtgtgattt caccggaatt attttacatt tctgcattcg cattaagttc
     7681 tgtagtaggt attgcgttag gcagtagttt aaccacaacc gccaccatcg gtgttgcttt
     7741 tatcggtatg gcttctgctt tcggtgcgaa ccctgcgatt gtagcaggtg cggtggtttc
     7801 aggggcgttt tttggcgata aaacctctcc actttcagac actactacca ttgctgcttc
     7861 cgtagtgggc attgatatgt tcgctcatat ccgtaatatg ctttatacca ccatccctgc
     7921 gtggatttta acagcattat tcctttggtt tttaaccgaa cctactgatg cacaaaattt
     7981 agcaaatata gagaacctaa aaaccaattt gcaagaaaca ggattaatac acggctactc
     8041 tttgctacct tttgttacgt taattttaat ggcaattaag cgtgttaatt caatttatat
     8101 tattgtaact acagtaacac tctcgctaat tattacctat atccacagtt cgccaaccct
     8161 tgaacagcta gggaattatt tctttaccag ctataaacct gttgaaggcg tagatttagg
     8221 cgaagtggca tcattagtct ctcgtggtgg cattaatagt atgttcttta ccatcactat
     8281 tgtattgctt gcgttaagtt taggtggtct tttacaaatc ctcggcattt taccggcact
     8341 acttaacggc attagcggct ttttaaccag cacaggacgt gttactcttg tagttgccag
     8401 cactgcggtg ggaattaacg tcttaatcgg tgagcaatat ttgagcttat tgttatcagg
     8461 taatactttc cgctcaactt atgagcgttt aggcctagaa gtgaaaaact tatctcgcac
     8521 cattgaagat gcaggcacgg taatcaaccc attagtccct tggggcgtgt gcggtgtgtt
     8581 tatcagtagc gtattaaatg taccggtgtt agattatcta ccatacgcat tcttctgtta
     8641 tatctgttta atccttacct taatttttgg atttaccggg gtaacaatca gtaaaaaagc
     8701 taactaatat gtgaaacatg cctacaaatt tgtaggcatt ttcttttcat tagagctgaa
     8761 gaacagcttg tttggctaac tctctggcga gtttatcaat atgcaataca tcagcaattt
     8821 cactgacttg aattggagca atattttcca ccaccgttct attcacgttc acaatatccg
     8881 taaagcggat ttgctcgttt aaaaacgcct caacggcaat ttcattggcg gcattcatcg
     8941 ctgttgtcgc atactgtcct tctgcaaaag cctcaatcgc taattttaag ttcgggtaac
     9001 gggcaaaatc cgactcgata aaggttagct cttttaattt aaagaaatct aatggtggca
     9061 caccagcatt aattcggttt ggatatgcca tcgtgtgagc aatcggcgta cacatatccg
     9121 ggttgcccat ttgagcaatc accgagccat caatataacg caccatcgaa tgaataattg
     9181 attgtgggtg aatgatgata tccatttcct cggctgttgc attaaacaac cagcgggctt
     9241 caatatactc caagcctttg ttcatcattg tggctgaatc gactgaaatt ttcttaccca
     9301 tagaccaatt ggggtgagca actgcttgag ccggtgtaat cgcatcaaat tcatttaatg
     9361 gcttagtaca gaacggtcca cccgatcctg ttaaaataat tttactgaca cccagttcag
     9421 cgagtggaca aaacccgact ttttgctgtg cctccggtgg aagcgattgg aaaatcgcat
     9481 tgtgttcact atctaccggt aagagcttcg caccggattt acgagcctca tcaataaaaa
     9541 tttgcccaca ggttaccagc gattctttat ttgccaacaa tacggtttta cccgctttca
     9601 ccgcagaaag tgtcggtaat aaccctgctg ctcctacaat agcagccatt accatatcca
     9661 cttcggaatg tgcagcaagc tcacaaatag ctgcttgacc ggctaacact tcagttttag
     9721 accccaaagt tgccaatgcc gtttttaatg cttgtgcgga tttttcatca tccattgccg
     9781 caaaagtcgg ctgatattga cgacattgct ccagcattaa ttcaacattc tttccaccaa
     9841 ctaacgcaaa cacctcatat tggtttgggt tatgctcaac taccgataaa gtgcttttac
     9901 cgatagaacc tgttgaacct aaaatcacta attttttcat tttgtttctc tttatctaat
     9961 gactgataag ggcgaaagat ttttcgcccc tacgccgtaa ttatgcggat actcattcgt
    10021 tgtttgtaaa ttgtttagca aaaacaaccg cttgtagatt aactcgaaag ccgttttgct
    10081 tcgataacat catctaattg agcgatacga gccaataatt tattcaaaat ttcaacgttg
    10141 ttaagctgaa tttccatatc aatggtggca aggctacgtt tcacatcggt tcggctggaa
    10201 acgcctaaca cgtttacttt ttcatttgcc ataattgcac tgacatctcg cagtaagccg
    10261 ttgcggtcat tggcaatcac tcgaatggtt aagcttaaac cagaggtata gccttcgccc
    10321 caatccgcct ccacaactcg ttctgggttg agactttgta attcaaacag ttgctcacaa
    10381 tctgcacggt gaatcgaaat ccctctgcct tgtgtgatgt aacccacgat ttcatcaccc
    10441 ggaatcggct ggcagcaacg ggcaatggaa tgcattaagt tccctacgcc ctctacaatc
    10501 acatagcctt tgcttggctt cggctgattg gtgtttttat tcacctgttt aagtaccgct
    10561 tcgtcttctt gttcggcggt cggtttaatt aattttgatt gcaagaagtg cattaactga
    10621 tgaagacgaa tatcgccacc gccaataccg gcatagagat catcaaactg ttttaagtta
    10681 tagcgaggca gagcatattc ttcaatttgc ttgagcgtaa aaccgaattt ttccatttcg
    10741 gcttcaaggg cctctttgcc gattggaata tttttctctc ggtctaattt tttaaaccac
    10801 gcaataattt ttgagcgagc tttgcctgta ttcacaaaac cggtattcgg attgagccaa
    10861 tctcggcttg ggttcggctc tttttgggtg ataatttcaa cctgatcgcc catttgcaga
    10921 tgataagtga atggcacaat ccgccctgcc acttttgctc caatgcaacg atgcccgatt
    10981 tcactatgaa ttgcataagc aaaatcaagc ggcgtagctt tttgcggtaa atcgatcacc
    11041 tcacctctcg gggtaaaaac ataaacacga tcatcaaaaa tttgtgaccg catttccgcc
    11101 accatatcgc ccgaatcggc aagatcattt tgccactcaa ggagtttacg caaccagaca
    11161 attttttctt cgtagcccga acgccctgcg gttgctccct ctttgtattt ccagtgagcc
    11221 gccaccccaa gctcggcatc atcgtgcatt tgctgggtgc gaatttgcac ttcgatggtt
    11281 ttatcgccct cgcctaatac aacggtgtgt aatgattgat agccgttagg tttcgggtta
    11341 gcaatataat catcaaaatg ttccggcaaa tgggtatatt tggtgtgaat aatgcctaat
    11401 gccgtgtagc aatcttctag tgttggcact attacccgca ctgcccgaat atcgaaaagc
    11461 tgattaaatc tcaaccgttt tttctgcatt ttcttccaaa tgctgtagat atgtttcggg
    11521 cgtccataaa tctgcacatt cttgatttca ggacttaaac aagcggtcag atctgctaca
    11581 aaatttgcaa tataacgctc acgatccaca cggcgttcgc ccaattcaaa tttagcaatt
    11641 tgagtgtaag agacagggtg taaaatgcgg aaacagtaat cttccagctc ccatttgagc
    11701 tgcccgatac ccaatcgatt ggcaagcggt gcataaacgt gagaacattc tttagcggcg
    11761 agaacggtat cttcttcgga atggtggtta ctatcacgca ggtaggcaat tcgttcggca
    11821 agtttaatca ccacacagcg gaaatcgtcc accatcgcca acaacatgcg acggatattg
    11881 tcgatttgca gatccgacac attattggca ctaagctggc ggatattctc catttcgagt
    11941 acgcctttaa ctaagttttt gatgtcgtta ctgaaatctt cttttaattg caccaagtcg
    12001 atcacattgt gttttaccag aggaaaaagc attgcggcaa ctaagctgtc atcgtccata
    12061 ttcaagccgt gcaatacttc aaccatctca atgccatacc acatcagact atagtcggta
    12121 tccagttttt cctgtgcata actccacgca ttgtgaatct gctcaaaagt cacaggagac
    12181 atattcaggc ttgcacacca actcgacaac tcaaaattat tcggttcaag ttgatgggaa
    12241 taacgaattg ctaccataag gcctccttgt aaatttttgt ggaaattaga ccgcttgtac
    12301 agcttaaaat ggagttttat tatacgtgga aattcagaaa aaatttaccc ctaatcgaaa
    12361 atttaatcca agttatctta tcctctttac tccttaagag gtagcaacct tgtgataaat
    12421 caaacctatc aacacaatca tttgcacgac ttcacgctct tgattaaaat acttgcgttt
    12481 ttattttgat ctttattttt gtgggtagca atgacaattt tagctttagg aatcaaccat
    12541 aaaaccgcct ctgtgggttt aagagagaaa gtggcgtttg tagatgacaa acgtaaactg
    12601 gctcttgaac aaatccaaac gagcggattg gctgaaagcg tggtcattct ctctacctgc
    12661 aaccgcaccg agctctactt ccaccagcct aatatttctc cccgtgaaga gagcgaggaa
    12721 aatatccaat ggcgtgaaca atgtttccgc tggtttgccc aaattcatca acttgatgaa
    12781 agcgagttgc gtgaatgttt atattttaaa caaaatttag aagccgctaa ccacttaatg
    12841 aaagtggcaa gcggattaga ttcactaatt ttaggcgaac cacaaatttt agggcaggtt
    12901 aaacaagcct atcaatatag cgaagatttc taccaacatc agcaaaaagc gatgttcacc
    12961 aagctatcac gcttgttcca acgcaccttt gccaccgcaa aacgagtacg ttccgaaacc
    13021 gaaatcggca gccaagcggt gtcagttgct tatgcagctt gcggtttagc ccatcaaatt
    13081 ttcgataatt ttggcaagtt acgctttttg ctggtagggg caggcgaaac cattgaatta
    13141 gtcgcccgct atttaattca acacggggcg aaaaatataa tgttggcaaa ccgcacccat
    13201 attcgggcgg aaatgttggc ggaaaaacta aacgctccaa tgcaaattct gtcgttaagt
    13261 gctttgcaac tcggtttaaa ccaagctgat gtagttatta gctccaccgg tagccctgat
    13321 accctaatca gtaaagaaat ggtggaaatt gcgcaaaaac aacgccaatt tgacccactt
    13381 ttgctgattg atattgccgt tcctcgtgat attgacgaaa aagccggcga cttagagggc
    13441 gtttacgctt atagtgttga tgatttacaa aatattatcc aacgcaatat ggctcagcgt
    13501 gaagaagccg ccaaacaagc cgctgaaatc gtcaaagagg aaagcaaagc attttttgaa
    13561 tggttaaaac aacagcagtc tagcgattta atcaaacgct atcgtcaaga tgcggagcaa
    13621 acccgccaag aattgttgga gaaagcccta caagcaatca gtcaagggca agacagccaa
    13681 aaagtattaa gtgagctaag ctacaaactc accaaccagt tactacacgc cccaacccaa
    13741 gcgttacaag caatggcaaa aagtggcaac tccaccggct tacaaagttt ttcaagagca
    13801 ttacggctag aagaatgatg ctcagcacat tcttttaggc atatttgcaa aaaattgaac
    13861 atttttaacc gcttgcggat gcacgccgtg cgtccctccc cgtgttcccg atcttcttct
    13921 aatttcaact tccccaaatt tccgctataa taagccaatt tttgcaacca ttttgcaaat
    13981 aaccggatat agaggataca aaatgactcc tgttgttgcc ctagtgggca gacctaatgt
    14041 cggcaaatcg actttattta accgtttaac tcgtactcga gatgccttag tagcagattt
    14101 ccccggctta acccgagacc gtaaatatgg tcacgctaat attgccggct acgatttcat
    14161 tgtaatcgac accggtggta ttgatggtac agaggaaggc gtggaagaaa aaatggcgga
    14221 acaatcttta ttggcgattg aagaagccga tgtggtgctt ttcttggttg atgcccgagc
    14281 cggattatta cctgccgatg tcggcattgc tcaatattta cgccaacgtg aaaaaaccac
    14341 ggtggtggtt gccaataaaa ccgatggtat tgatgccgac tctcactgtg ccgaattcta
    14401 tcaattaggc ttaggcgaag tagaacaaat tgctgcagca caagggcgtg gggttacgca
    14461 gcttatcgag caagtgttag ctcctttagg cgaacagcta aatgccgagc aatttaacca
    14521 agaacaagcg ggcgaaaatg ctaaaaatgt tgcaaatgaa gaaacagacg aatgggatac
    14581 cgaattcgac tttgagaacg aagaagatgc ggcattatta gatgaagcct tggcagaaga
    14641 agcagaagag tcgattgaag ataaaaatat caaaattgcg attgtcggtc gtccgaatgt
    14701 tggtaaatca acactgacca accgcatttt aggcgaagag cgtgtggtag tttatgatat
    14761 gccaggaaca acccgtgatt ctatttatat tccaatggag cgtgacggtc agcaatatac
    14821 catcattgat accgccggtg tgcgtaaacg tggcaaagtg aatttagcgg ttgagaaatt
    14881 ctcggtaatt aaaaccttgc aagccattca agatgcaaac gtggtgctat taactattga
    14941 tgcccgagaa ggtatttccg atcaggattt atcactactc ggctttattc taaatgctgg
    15001 tcgttcattg gtgattgtgg taaacaaatg ggacggcttg tcgcaagata ttaaagatca
    15061 agtgaaatca gagttagatc gccgtttaga ttttattgat tttgccagag tgcattttat
    15121 ctcggcattg cacggctcag gcgtggggaa tttgtttgat tcagtaaaag aagcctacgc
    15181 ttgtgcaacg caaaaaacct cgacctcaat gctgactcgc attttgcgta tggctgctga
    15241 tgaataccaa ccaccattgg ttaatggtcg ccgagtgaaa ttaaaatatg ctcacccggg
    15301 tggctacaac ccaccaatta ttgtgattca cggcaatcag gttgaaaaat tagcggattc
    15361 ttacaaacgt tatttaagta attatttccg taaaagttta aaaattatcg gctcgccaat
    15421 tcggatttta ttccaagaag gcaataatcc ttttgccggt aagaaaaaca aactgacacc
    15481 aagccaatta cgcaaacgta aacgtttgat gaagtttatt aagaaaagta agaaataata
    15541 tctaacgata gcgcaagcgt ccacgcttgt gctaaaacta ataatggcac aagcgtggac
    15601 gcttgcgcca tcatttaaca gagaatatta actatgatga tgcaatactc tccaaaattt
    15661 aataatgcta aagtgctggt gcttggcgat gtaatgttag accgttactg gttcggggca
    15721 accaatcgaa tttcgcctga agcccctgtg ccggtggtga aagtacaaga tattgaagag
    15781 cgtgccggcg gtgcggcaaa cgtggcgatg aacatcgcca gtcttgacgt accggtaaca
    15841 ttacacggct taattggcga tgatgatgcc gggcgtgctt tagataaatt acttaattcc
    15901 caccagatcc aaaatcattg tgtggcgatt aattcgcacc caaccatcac aaaattgcgg
    15961 attctttccc gccatcagca attattgcgg ttggattttg aagagggttt tcacaatgta
    16021 gattctaccg ccctacttgc aaaactttcg caagaaatta ccgcttacgg agcattaatt
    16081 ctctcggatt acggcaaagg cacattggaa tcggtgcaac aaatgatcca aattgcccgc
    16141 aatgctaagg tgccggtgct gatcgacccg aaaggcacag actttgaacg ctatcgtggg
    16201 gcaacattgc ttacaccaaa tatgtcggaa tttgaagcgg tggttgggca ttgtaaagat
    16261 gaggatgaga ttgtcagcaa agggttaaaa ctgattgccg actatgattt atctgcacta
    16321 ttagttaccc gttcggaaaa aggaatgacc ttacttcgcc cgaaccacga gccttatcat
    16381 ctgccgaccc aagccaaaga ggtttatgat gtaaccggtg caggcgatac cgtaatcagt
    16441 gtattagcca ctgcgattgc cgatggcaga gcctatgaag aagcctgcta tttagccaat
    16501 gctgccgccg gtgtagtcgt aggtaaatta gggacttcaa ccgtcagccc ggttgagctg
    16561 gaaaatgcga ttcaccaacg cactgccacc ggttttggct tggtttctga agccgaatta
    16621 aaagcgattg tggaagaagc gaaaaaacgt ggcgaaaaaa tcgtgatgac gaacggttgc
    16681 tttgatattc ttcaccccgg tcacgtttcc tatttggaaa acgcccgtaa attaggcgac
    16741 cgattaattg tagccgtcaa taccgatgct tcggtaaaac gcctaaaagg cgaagctcgc
    16801 ccgattaatg atttagctgc ccgtatggca gttttagctg gtttagcctc tgtagattgg
    16861 gttgtgcctt ttgatgaaga tacaccacaa cgtttaatca gtgaaatcct acctgattta
    16921 ttggtaaaag gtggtgatta caagccggaa gagattgccg gcagccaaga agtatgggca
    16981 aacggcggcg atgtgcgagt attgaacttt gaaaacggct gttctaccac taatgtgatc
    17041 aagaaaattc aagcactata atggtaataa gcgaatgacg agagaaaaaa tcattattga
    17101 taccgagcaa tttcagcgca caatatctcg tatttcacat caaattattg aaaaacacgg
    17161 tgatttgcaa gatgtagtga ttgtcggcat caaacgtcga ggagctgaaa ttgccgagtt
    17221 aatccaaaaa cgcattgaag atttagctca aacacgcttg ccttatatgg cgttagatat
    17281 tacgttctac cgtgatgacc tagacttaat ttatcaagat cccgtattta tgggagccga
    17341 taatcagcta aatctgaacg gtaaaaaagt gattttggtt gatgatgttc tgtttaccgg
    17401 cagaaccatt cgggcggcac ttgatgcact cttagatttt ggcagagctg ccagaattga
    17461 actggtgatt tttgtcgata gaggtcatcg ggaattgcct atccgagccg attatgtcgg
    17521 aaaaaatatt ccaacggcta aaaacgagca aattcaggtg caaacgctct cgtatgatgg
    17581 gattaaccaa gtggtcttag ttcctgctgc caataaagaa agtgcttaaa ttaagtgaac
    17641 cttttcgcta aacttatctc taaggaaaag ttactgctta attacctcca ttaggattgt
    17701 tataaaaatg aaattagtca aatcattatt tgctgttgcc gttgcaacaa tgagtcttac
    17761 ccaaaccgcc cacgcatttg aagaaagagt ggtggcgtta gtgaatgata cccctattat
    17821 gcaaagccag gttcaacgtg tgcttggcaa gaaaaaggcg acagaaactg ctcaacgtgc
    17881 ggctattgat caagtgattg atgatatgtt ggtgcaacag gcaatgaaag aagccggcgt
    17941 taaagtcagc ccagctgcgg taaaccaagc ggttgaaaat gtggcaattc aaaacggtat
    18001 tacttacggt cagttattag atgcgttaga ttatcaaggt attacacttg aacaattcaa
    18061 acgtaatatt gctcaacaaa tggcaatgga acaagttcgc catatcagca ttagcaaatc
    18121 gattcaggtt gcaccacagc aagtgcaagc tctggcgaaa gagttaatgg cgaaagataa
    18181 agcagcaggc aaattgaaaa ccgtttccgg caaagagcat cgaattagtc atattttgct
    18241 aaaaaccaac ccgattttaa atgatgctca agccaaagca aaacttagca gcttaactgc
    18301 tgatattaac tcaggcaaaa tcagctttga agaagcggca aaagcccatt ctgttgatta
    18361 cgcctcaggg acagacggtg gagatttagg ttttaacttc ttagaaattt acgatccggc
    18421 atttgcgaat gttgcatcaa aaatcaaacc aaaccaaatt tctgctccgt ttaaatcgca
    18481 atttggttgg catattttaa aagtcaccga cactcgtaac ggagacagaa ccgaagatgc
    18541 ttaccaccaa cgtgcttatc agcaactggt gaacaagcaa gccgaagaag cctctaaaga
    18601 ttgggtaaaa gcgcttagaa aaaccgccaa tattcaattg tcggtcaata attaaaattt
    18661 ttaagaacct cgcataatgc ggggttcttt tcttatttca aaaaggataa caatgagttc
    18721 tacaaattcc aaaaaacatt taggtcatac cgctcgtaag cgtttcgggc aaaacttttt
    18781 gcacgatatg aacattattc acagcatagt ttcagccatt aacccgaaaa acggacaatt
    18841 tttattagaa atcggtccgg gtttaggagc attaaccgag ccggttgccg aattggtaga
    18901 taaactcacg gtggttgaaa ttgaccgtga tttagcagag cgtttacgcc accacccgtt
    18961 tttaaaccaa aaactcacca ttattgagca agatgcccta cgctttaatg tccgtgacta
    19021 ttttaaccaa ctcaacttag acgaagactc ggtgcgtgtg tttggtaact taccttacaa
    19081 catttctacg cccttgatat tccacctgct gacctttcac gacttaattc aagatatgca
    19141 ctttatgttg caaaaagagg tggtgaaacg tttatgtgcc gcgccaaaca gtaaagcata
    19201 cgggcgttta accattatgg ctcaatacta ttgccaagta attccggtgc tggaagtgcc
    19261 gccaaccgcc tttaaaccgg caccaaaagt cgattctgcg gtagtgcgtt tagtgcctta
    19321 taaccaactg ccttatccgg caaaagatgt gtattggctc aatcgggtca cgactcaagc
    19381 ctttaaccaa cgccgtaaaa ccttacgcaa tgctctttca accctgttta gtgcggagca
    19441 actggaagcc ttaggcgttg atttaaccgc ccgagcggaa aaccttacca ttgccgatta
    19501 tgtgcgtttg gctaactggc tacacgacaa cccgccggca attgatacaa ccgaagaatt
    19561 ggttgatgaa gattgctaat caataaatta caagcggtca tttttctacc aagatttgca
    19621 aatctttagt gaaaaatggc cgcttgttta aatcagcttt acttgttttg ctgttactta
    19681 gttttgatgt tacttggtgc cgaaaatttt atcccctgca tcgcctaagc ctggaatgat
    19741 atagccgttt tcgtttaagt gactatcgat tgaggcggtg tagagttcaa catctgggtg
    19801 ggcggcttct aatactttaa tcccctctgg agcggcaact aacactagca ctttaatctg
    19861 cttacagcct gcttttttca ggaggtcaat ggttgctacc attgagccac cggttgcaag
    19921 cattgggtcg gtgataatgg ctaaacgctc atcaacatcg ttggctagtt tggagaaata
    19981 cggcacgggc tttaaggttt cttcgtcacg ataaatccca accacactaa ttctggcact
    20041 tggaatatgc tctaacacac cgtccatcat acctaagcca gcacgcagaa tcggcacaac
    20101 agtcactttt ttgcctttaa ttcgctcaac cgcaattttc tctccgctcc agccattgat
    20161 ctccacactt tccatttcca gatctttggt tgcttcgtaa gtaagcagac taccaatttc
    20221 attcgctagc tcacggaaat ctttggtgct gatttccgct tcacgcatta agcccaattt
    20281 atgctttaca agcgggtgtt ttacttcaac aattttcatt ttaagctcct taattagggt
    20341 aatttaagcc ttattcacct ttgctaattc ttcgtctctt aacactcggc gaagaatttt
    20401 accaacatta ctttttggta attcgtcacg gaattcgatg tctcttggaa ttttgtagcc
    20461 ggttaagaat tgacgacaat aggttctcaa ttcatctcgg gttaaacttt catctttttt
    20521 ggttacaaaa attttaatgc tttcgccgga agcctcactc ggcacaccaa tcgccactac
    20581 ttcattcact tttgggtttt gggaaatcac ttcttcaatt tcggttgggt acacattaaa
    20641 accggagaca ataatcatat cttttttacg atcaacaatg cgtaagttca gatcatcccc
    20701 cattaccaca atgtcaccgg ttgccatcca accatcttgt agcacttgtg ccgtttcatc
    20761 aggacggttc caatagcctt gcattacttg cggccctttc accaccagct cgccccgttc
    20821 gccgagtggt acttctttgc cttcatcatc cacaatacga atgtcagtat ttggcactgg
    20881 cacgccaata gagccggaat attccaccga atcgccacgg gttgcactaa tcagcggaga
    20941 acattctgtc attccataac cttcgataat atgtacgccg gttaaatcgt gccagcgttt
    21001 tgccacgcta gattggatcg ctgcaccgcc acctacactt aattttaggt gtgaaaaatc
    21061 aacctcttta aaactttcgt gattaagtaa tgcattaaac aatgtattta ccccagtaat
    21121 cactgaaact ttgtactttt ttaattcttt aataaagcct ggaatatcgc gcggattagt
    21181 aattaataat ccggtaagtc ctaattcgat aaagagcaag caattcatcg aaagtgctaa
    21241 gacgtgatac agcggtaaag gtaccaatcc aatcatattg gcagaattac ccaacaatgg
    21301 ctcaacaatc cattttgcct gcatcatatt agcaattaag ttttgatgag tcagcattgc
    21361 ccctttcgct acacctgtag taccgccagt gtattgtaaa aaggcgagat cttggctgac
    21421 aatattcggt tttacatatt gacgctgttt gccaatgctt aaggcttcac ggaagctgac
    21481 cgcatgtggc aatttatatt tcggcactaa ttttttgatg tatttcacca caaaattgac
    21541 taaggtgcgt ttgccgaatg aaagttggtc gcccattcgg gtcaaaataa cgtgcttaat
    21601 ctcagtatta aagacaattt tttctaaggt agtcgcgaag ttcgagacta tcacaatcgc
    21661 tttcgcacca ctgtcattta attgatgttc taattcacgc ggcgtataaa gcgggttgac
    21721 attcaccacc acaagcccgg cacgtaatgc gccaaataaa gcgatcggat attgcaataa
    21781 attcggcatc atcaatgcga ttcgatcacc ttttttcagg cgtaattcat tttgcaaata
    21841 agcggcaaac gagcggcttc gttcttccaa tttacggaac gttaaaacct gccccatatt
    21901 aatataagct ggaatatcag ggtggcgttg tatcgctgct tcaaacattt ccactaaaga
    21961 ttcatattga tctgtttcaa tcgtttttgg tgaataaggc ggataattat ttagccatat
    22021 tttttccatt atagtctcct aaaaccttac ctaaaaagat aggtgaaaaa tttgcttgtt
    22081 tgctgtgttt atgaatcaat tttcttacag cccagaatgt tatacctgtt gaaagcaaca
    22141 ttataaataa aacaataagc tcatttgcaa aaaattgcga aaatccgacc gctgtcatca
    22201 caagaaccag cagaggaatg ccataaatta aaaacacgct tttgagcaag atattttcag
    22261 ctaaccctag ttcaacctta tcgcctactt ttaggggttc atctacctca atacgaaaac
    22321 gagggtcaat tttttcacct gctaatgctg ataatgcttt gctgccacaa gcagactgag
    22381 ctgcacagcc accacagcta cttttagcat aacattgtac aatagccaca ccattctgat
    22441 attccactac cgtagcctgt tcgatcatca tggctgagct ccttccttaa aaccaatatt
    22501 gttcataatt tgcttagccg attgcaacgg cacatcacca ataatcacaa tatctctatc
    22561 attaatcgtt tgatttaaaa tcgttaattt accttgttgc caagcgtact cattaaaact
    22621 cacgccttgg ctaggcataa tataaatagt taatgtagat aaaccatcac tatatactct
    22681 gctatctaca tactcacttt ccacttgata tgtcgcacct gtgactgttt gttgctgaat
    22741 taacctaaac cctgtcggca accacttcac ctgccaacca tcagacttag gaaattcttc
    22801 ttttttcact gtcgttaatg ttggtaacat tagtgattct attacatttg caatcctatc
    22861 caattctgga gattgataaa gttgcaaaac tctgaattct tctaatacca aattatccat
    22921 attcaagact tggcttttaa gtaataaatg tgtttcttca tcaatccata gtatataggc
    22981 atatcttaaa gtatcatttg gcttcatacg aattaccttt gtaattcggt cagaaatacg
    23041 atctttacca gcatctaaaa aggcataacc ttttaaattt gcataatctg tgtaaagtac
    23101 attagggagg ttatctaaaa tctgtgaact atttaaacta aaaggacgaa aattataccc
    23161 caaatagctg accttgttat tttttaaaat aatttcttct cggctgtgat ctaaattcag
    23221 taattgggca tattctttat tatgggtaaa agcatgacgt aaacggaaag attctgtatc
    23281 acttccctgt tgaagaatat aaactaattc ataagtggat tttttatgtg cttctgacat
    23341 agccgttaaa tatgctaatg gtgttttaat agttgattct gcccaagcaa atgtatagca
    23401 ggcagaaata acccaaaaca acaataagta tttttttatc ttaaaaagca tatttattct
    23461 caagccaaat taacttaggt tattttagca aaaaattttc actatttcgc tacttgttgg
    23521 ttatttctta ttttttcgct acatttacat acaaaaagcc acataattat ggataaatta
    23581 tgtggctttc agtaacttat tttacctatt atttttgtat atttggctct gattctgtat
    23641 tggaagaagt ttgtgctttt tgcttttctt cctcagataa ggttaaattt ttaacgttat
    23701 tagtacggcg ttgtaactca tggttttcta ataacgcatt aatcttacgc tgttgaagct
    23761 ctaactgttc ttctgttggt tgatcttgaa ctggcgcatt ataacttacc gcttcaactg
    23821 aattgctaaa tggcaatgtt tgtaatactg gttgttgcgt ttgagccatt tcatcactcg
    23881 cattcattga attaacccct aataccgcaa ctaagcacac tgaagctgca ataccggctt
    23941 gcataattgg agttccccaa cgcttgagct tgagcaataa gcctttagat ttctctacat
    24001 ttgcttgaga ttcaatttct tcattttcca gtaatgcttc cattttagca gagaaatctg
    24061 tccctaaaat gatctcatca ccttgcatca cactacgaat agtgtgataa cttgcccatt
    24121 tttgctgtaa ttcagggctg ttagttaatg tttctacaaa tgctgtatca acattttgcc
    24181 catccatata agctgaaagg cgttcatttt gttgagtgat gcgttcttgc ataattgact
    24241 ccaatgattt aaatctcttc taaaataatc tttctatcta ttgcataagt ggcttgattt
    24301 tagcatcaat cgcttccctt gctcggaaaa tacgggaacg tacagtacca accggacatt
    24361 ccatcacttc tgcgatttct tcgtaactta gcccttccaa ttctcttaaa cgaatggcgg
    24421 ttttaagctc atcaggtaaa ttttcaatcg tatcaaacac gactttcttt aattcctcgg
    24481 ataatatcaa attctctggc gtatcacttt cacgaagttg cgcgcctaca tcataacttt
    24541 cagcgtcttc ggcaagaata tcttcttttg gcggtcttct gcctaaagag gttaaatgat
    24601 ttttcgcagt attcactgca atgcgataaa gccacgtata aaaagcactt tcgccacgga
    24661 aagactctaa agaacgatag gctttaataa aagattcttg cacaatgtcc ggaatatcat
    24721 ctcttgcaac ataacgagtc agcaaaccgg ctactctatt ttgataacgt acaacaagta
    24781 aattaaatgc ttttttatca cctttttgtg ctttttcgac taattcttgg tcggtgatct
    24841 gttcgctcaa ctgcggactc tcctaaatat caatatgtca ttttccattg cacactttgt
    24901 tagtgtgtgc tttatgacaa aagttcaaaa aatttgaaaa attgtgtaat tttttacatt
    24961 cctagataaa acctacttaa taaactaagc aagcggtcgg attttagcga aattttgcaa
    25021 aaatttcaaa aaatccgacc gcttgtctat gttatgcttt cgctctataa gttatttctt
    25081 cgcttttaaa tctgccactt tttgtgaacg gtaaatcgca ttaatggcgt gaattaaagc
    25141 tcgggctgaa gattccacaa tatcggttgc caaacccaca ccgtggaatt ttcgaccttc
    25201 atattccact acaatatcca cttgccctaa tgcttctgct ccttcgcctt tcgcagtcag
    25261 attatagtgc gacattttta gctccattcc cacaatgttc ataatggcat tgtaagttgc
    25321 ctcaactgga ccatttcccc cttgagatgt agcactttga cgaacgccgt ctaattccac
    25381 ctgtacaaaa gccgaagccg gtaaaccgct gattagttgg gtggtaatta catctaattt
    25441 taagcggtct tcatcgcctt gctgcatatc aatgaaggca agagcttcta agtcataatc
    25501 aaacacttgc ccttttttat cagcaagttg caagaaggcc tcataaagtt tatctaaatc
    25561 gtaatccgtt tccgtataac ccatatcagc catatgccct ttcaccgctg ctctacctga
    25621 acgggcagtt aaattcagct tctctttttt caagccgatg gtttccggcg acataatttc
    25681 ataagtattt ttatttttca gcataccgtc ttggtgaata cctgaagagt gagcaaaggc
    25741 attgctaccg acaatcgctt tgttcggctg aatcggcata ttgcaaagct ggctgaccat
    25801 ttggctgaca cggtgaattt cttgggtatt aatgcgggta tcaaccccca taaattcttg
    25861 gcgggtttta attgccatta ccacttcttc aagagcggtg ttaccggcac gttcgccaat
    25921 accgttgata gtacactcaa tctgtctcgc tccgttttgc accgctgtca atgagttggc
    25981 ggtagccata cctaaatcgt tatggcagtg aacagagaca atcgctttat cgatattcgg
    26041 aacacggttc atcacttggt ggataatatt gccgtattca ttcggtaaac agaaaccgac
    26101 tgtatccgga atattcactg tggttgcacc ggcattaatg gcagcttcta caatgcggca
    26161 gatgttatca attccagtac gcccagcatc ttcacaggaa aactcaacat catcagtata
    26221 atttctggcg cgttttaccg ctgcaaccgc catttcgact acatcatcaa atgaacgttt
    26281 taatttagcc tcaacgtgta atgccgagct ggcaataaag gtatggatac ggaatgcttc
    26341 tgctactttt aaggcttcat aagctgcatc aatgtctttt gcaaccgctc gggaaagcgc
    26401 cgccacccgg ctgtttttga tatgacgagc gatggtttgt actgattcaa aatcgcccgc
    26461 tgaagaaact ggaaaaccga cttccatcac atcaacccct aaacgttcta aagcaagggc
    26521 aatttgcaat ttttctttta cggttaaact tgcttttaat gattgctcac catcacgtaa
    26581 agtcgtatca aaaataataa tgttattgtt gctcatagaa gcctcttttt aggtgttttt
    26641 atttgataaa aaaagcccgc attttaaggt gcgggcaaga atgtggatag tccatttact
    26701 ttttccaatc aatctttatc ctcgcacaaa atacgataac agagataatc ggagtaaggt
    26761 aaatttttcc atgttgtgtt gtgtgtttat gtttgcattg ttattcagta gttgaggtgt
    26821 atattatcct tatccaaatc aatgtcaata gcatttttct cttcacacaa ccgtttacca
    26881 gcccattaat tcaagtcata ccagattatt atattgaata aatccaactt attagtaaaa
    26941 aaaactattt catttaagca aaatgataca aagaattaat tttaataata aaacaaatat
    27001 ttagaattta atgatatttt tgtaaaaaaa tttgttttct ttgaagtttc agatcaaatt
    27061 tggtagcctt tgatattatt ttattgttag gaatagatta tgactctccc atttcgtgca
    27121 attatttccg atcttgatgg cacactatta aatgccaatc ataaaattgg tgatttcact
    27181 attgatacat tagaacaact atcaaaaaaa ggtgttgata tttttttcgc caccggtcgc
    27241 aatcatccag atgtgtctca gattattggg aaagtaaacg ttaaaaatgc aatgttagtc
    27301 acttcaaacg gggcaagagc caacaattta gagggtaaaa aaattctcaa ccattattta
    27361 ccggaagatc ttgcctttga attaatgaac attgaatttg acgaccaaaa tgtgtgctta
    27421 aacagctatc aaggcgatga atggtttatc aataaagaga ttgcagagtt aaaaaaattc
    27481 catcaagatt caggatttag ctatcaagta gtaaattttg ctcatcatca cggtcgaaat
    27541 accgagaaag tgttttttat cggcaaaact gctgaagctt tagtaccgat tgaacattat
    27601 attcgggata cctatggcga ccgtgtgcaa atgacctact ctgccctact ttgccttgaa
    27661 gtgatggcaa aaggagtgtg taaagccaat gctctcgccg aattggtcaa attacggggc
    27721 tacaccttag ccgattgtat cgcttttggt gacggtatga atgatgtgga aatgctaagt
    27781 ttagccggca aaggttgttt aatgggtaat gccgatccac gtttaaaagc cgtattaccg
    27841 aataatgaag tgattggctt taacaaagat gaagcggtgg caagctatct tcgagccaca
    27901 tttggcattg tttagttgag agcaactttt ttgcaaattt ttgtctaaat ttgaccgctt
    27961 gtatatcatc aaggaacagt atgaaacgta aaacattagc cactcttgtg ctaattgccg
    28021 ctctcggaat aggcggctat ttttattatc agcaacaaac caataaaccg caaatccatt
    28081 acattaccca agctgtcaca cgggcagaaa ttaacaaaaa tgtgctggca accggttcgg
    28141 ttcgagcgaa taaacgcacc gaagtggggg ctcaagtttc gggcaaaatc caaaaactct
    28201 atgtcgagct cgggcaatcg gtgaagcaag gagatttaat tgcagatatt gattccagca
    28261 accagagcaa caccttaagc accgctcaag cccggctttc ttcttataaa gcacagctca
    28321 cctctgcaca ggttgcactt gaagtagctc aatcgaacta caaccgctta aataaactct
    28381 atcgggcaaa tagtgcctct ttaaacgatc ttgaaacggc taaaaatacc ttagcttcag
    28441 ccaaagccac ggtggataac attaaagcac aattaaaatc ggctgaaatt tcggtaaacg
    28501 atgctaaaac caacttaact tatacgcaaa ttatctcgcc aatggacggc gtgattgtgt
    28561 ctgtaccggt ttctgtcggg caaacggtca attccaatca aacttcgcca actattgtac
    28621 aggtagcaga tttatctaag atgctgatta aacttgaaat ttcggagggc gatattgcac
    28681 aggttaaagt cgggcaaaaa gttaagtttt ccacccttgc tgagccaaat cgagaatata
    28741 gtgctaccat tgattcagtt gatccggcac tcaccacgct gaccgataat aactacaccg
    28801 aacaatcggg taataccgaa gcggtttatt actacgctaa tgtttccgtt gataatgccg
    28861 ataacagttt acgcatcgga atgactactc aagcccaaat tactattgat aaacgggaac
    28921 aggttttagt tgtacccacc agcgtaatca aaaaacgtgg taaagagaat tatgtgttgg
    28981 tgctggaaaa tgagcagtcg gttgaaagaa aaatccaaat tggcatttcg gatacgcaaa
    29041 ataccgaagt attagcaggc ttaaatgagg gtgatcaagt gattgttacc caaagagcgg
    29101 atgatgaaaa aatcagcact gcacgtggac caagaatgtt ctaaattcta aacaacaagc
    29161 ggttaaattt tctgaacatt ttgcaaaaaa ttagaaaaat ttaaccgttt gtctattctt
    29221 tattaaaaca ttaaaacgtc tgtattaaaa cgaattttat ttttcgttat cgtttttcat
    29281 tttagcgaaa atcatcgcca aaatcgggcc tgaaatattg tgccagaagc taaataccgc
    29341 acttggcacc gcaatgatag ggttggcttt aaagtgtaat gcggcaagtg ctgcccccaa
    29401 tcctgagttt tgcatcccca cttcaatcgc caccgctttg ctgtctgcaa tcggtaattt
    29461 taataaacgg ctcgccaaat aaccgattaa ataacctaaa ccgttgtgta atgctactac
    29521 cgcaaaaatt aataaaccgg attcaataat acgatctcgg ctaactgaca ccaccgccgt
    29581 gacaattaat acgattgcaa tcactgaaat taatggcatt gtttggctaa attgctctac
    29641 tttttgtttg aaaatagtac gaatcaccac gcccacaaaa attggcaata gcaccatttg
    29701 caacaccgaa ataaacattg ccgttgcatt aatttctaac cattggctgg caaataaata
    29761 gaaaattgcc ggcgtaagca ctggggcaag taaagttgaa atagtggtac aagctaccga
    29821 taacgctgtg ttgccacgag ccaaataggt catcacgttt gaagaagtac cgcccgggca
    29881 acaaccaact aaaatcacac caatggctaa atctgcgggt aagttaaatg cttttgccaa
    29941 tccataagct acactcggca ttaccacgaa ttgagcgatc acgccgataa tcactgattt
    30001 cggatttttc gttacctcgg caaaatcttt gaaagttaag gttaacccca ttcccaacat
    30061 cacaatccct aacagataag gaatccaagg caggaattgc ttaaaggttt ccggaaattg
    30121 tgctgcgata aaagcaaaaa acaccaccca aatggcgaag gttttgctga taaattgtgt
    30181 aaattttaat aatgtttgca tatagcctct gtgtaattgt tataaaaagg ttactaggct
    30241 accttagttt gctttaaaag tgaataggag caataaacta ttagccataa gcaactcttt
    30301 ttaggtataa tcgctctgtt tatcaacaat aaaactaagg atatacgtat gaaaaacatc
    30361 aaactttctg ctatgagcct cacagcttta tttgtattaa ctgcttgttc gcacacgcat
    30421 aaaaacagcg atgagcatca tcaaatggtg ttgcaagagc aagctacatt agggataaac
    30481 tggatgcaac aatccggcga ataccaagct cttgcccatc aagcattcaa tgcagcgaaa
    30541 gtggcattcg accaagccaa agtgaaaaaa ggcaagaaaa aagccgtggt agtagattta
    30601 gatgaaacta tggtggacaa cagcctttat gccggctgga aagttaaaaa ccacaagccg
    30661 tttgatggtg aaagctggac tcgttgggta aatgcccgcc aaaccggagc gattgccggt
    30721 gcagtggaat ttaacaacta cgttaatagc cacaaaggaa ctatgttcta tgtttcaaac
    30781 cgtaaagaca gcagtgaaaa agccggtact attgatgata tgaacaaatt aggctttagt
    30841 ggtgtgagcg aacaaacact cttgttgaaa aaagacaaat cgaacaaaac ccctcgcttt
    30901 gaagaaatcg aaaaacaagg ctatgaaatc gtgctctata ttggcgacaa cttaaacgat
    30961 ttcggcgatg cgacttaccg taaatcaaac gcggaacgcc gtgcattcgt agccgaaaac
    31021 agccaacaat tcggtaaaaa attcattatg ttaccgaacc cgaactatgg cgactgggaa
    31081 ggcggtttag ataaagatta ctacaaaggc aatgctgaaa gccgtgttaa aatccgccac
    31141 aatgcaatta aagcttggga cggcaaataa tccgtcctta atcaggttgt aaaaacgcga
    31201 aacaatgacc gcttgtcaag gctatgctct gcaagcggtc atttttgtat aaaactttgc
    31261 aaaagtttgt taggataaag caaaatccat tttctaagga aaccgataat gacagcaatg
    31321 aaaatttcac tttctactca aaacgcccct gaaatttggg gtaaaaatgc tattctttcc
    31381 gcaaatgatg cggggtttac cgtacataat ccgaacaacg atttagccac tattcaaaaa
    31441 gccgcccgca aaattaaaaa tcaaggtatt tcaaccgtgg agctgaccgg cgatagttgg
    31501 gatttaaaat cctgctgggc gtttcatcaa ggatttcatt cggtaaaaaa tcaaggctcg
    31561 gtacaatacc cagacttaga cgaacagcaa gccgaatttg ataaccgtgt aaaatgtgcc
    31621 actttcgtgc gtgaattaat taataagcca tcagataaat taacccctga aaaattagcc
    31681 gaacaagcgg tcgagtttat ccaaaaaatg gcaaaaccga atacggtttc ttaccagatc
    31741 attcgtggcg aagcattaca agaacaaggc tatcacggca tttggacagt tggaaaaggc
    31801 tctgccaatc cgcctgcatt cttagtatta gacttcaacc caaccaacga tgaaaacgca
    31861 caggtactgg cttgcttggt cggcaaaggc attacctttg acaccggcgg ttatagtcta
    31921 aaaccaagcg atagtatgag cacaatgcga accgatatgg gcggagcggc acttttaacc
    31981 ggctcacttg cttttgccat tgctcgaggc ttgaataaac gagtgaaact ctacctctgc
    32041 tgtgcggaaa atatggtcag ccacaatgcg ttcaaattag gcgatattat cacttaccgc
    32101 aatggcgtaa ctgttgaagt tttaaacacc gatgctgaag ggcgtttagt gcttgccgat
    32161 ggtttaattg atgcgtgcaa gcaaaatcca caatttatta ttgatgctgc aaccctcaca
    32221 ggagcagcaa aagtcgcagt cggcaatgac taccactcgg tgctttcaat ggacgacaac
    32281 ctaaccgaag cactatttaa gtctgccaaa acggaaaatg aaccgttctg gcgtttaccg
    32341 tttgaagaat ttcaccgtgg gcaaattgct tcttcctttg caaacatcgc caatatcggc
    32401 tctgtgccgg tgggggctgg ggcaagtacc gccaccgcat ttttatctta ctttgtagaa
    32461 aactaccagc aaaactggct acatattgac gcctctgcca cattccgcaa aacggcgagc
    32521 gatttatggg cggcaggagc aaccggactg ggaataagaa cagtagcgaa tttattgctt
    32581 tcaaaataga aacttcgtag gggcaaatca ctatttgctc cataaacaat taaataacta
    32641 tggctaaatc aaattacatt acccgttcag gctggcaagc cttagatcag gaattaaaat
    32701 ttctatggcg ggaagaacgc ccgaaagtga ctcaagcggt gtctgatgct gccgcattgg
    32761 gtgatcgaag cgaaaatgcc gaatatattt acggcaaacg ccgtttgaga gaaatcgacc
    32821 gccgtgtgcg ttttttatct aagcggctag aggtgctaca aattgttgat tatcacccta
    32881 aacaagaagg caaagtcttt ttcggcgctt gggtggaatt agaaaatgaa gagggcgagg
    32941 caaaacagta ccgtattgtt ggttgcgatg agttcgaccc agcaaaaaac tggatttcga
    33001 ttgactcccc tgtcgcccga gccttaatcg gcaaacaggt tgatgatgaa attagggtag
    33061 aaactccgct tggtaaagtg ttgctttata tcaataagat ttggtatgag caagcgagtt
    33121 aattacccat aaaggggcga aaaattttcg cccctacaca tatttaccca aacaaccgat
    33181 ccatttcttc aatcaactgc ttagtcggct tgtcaaaata aacgccgtta tcattttcgc
    33241 cgttcattgc ccttaagaac aagtaagcca ctccgccgaa gtctcgttca taatcgtatt
    33301 tttcacctaa acgggcagtt agataacggt gtaaggcgag agtgtaaagc agatattgca
    33361 aatcatagcg ttgtctgccc atttcccgtt gaatgtgttc tttcgcataa ttttgtggca
    33421 gatagcctaa gaaattcgat ttgtaatcaa ttaagtagaa tttctcctcc acttgcacga
    33481 tacaatctac aaagcctcgc acaaaacctt ctaattgcgg taattgcaat tcaggtaaag
    33541 tttttgctaa agggctatgt tgctttaata acgcatttaa ttgatgtaag gctttatcat
    33601 tttttaagcg taaatagaat tgccactcgt ttaatcgttt ggtttcatca atcgctttta
    33661 agcagaaatt tgcctctgcc aaaggggtag ttagaatacg ctcaaaccat aatgctgttg
    33721 gttcaatcca ttcctcactt agccctaatt gctcgcatac tttagcaaca gcctctctat
    33781 caatttgttc gttaaattgg caatgctcaa aaaagtgatg taacaccgtg cccactttaa
    33841 taccgtgtgg gaattggaat ggcgagaaag cggcagtttc acttgaataa cctgcctcaa
    33901 tggtttctat atgtacatct cgatcttggg ctaaatccat taccctttct gatttccctt
    33961 cactaaaacg ctcgtgttgg gcataaagtg aagtaaagct ggtaatttga ccaaccacac
    34021 ggatattgcc actaaaggtt tttgcttggt aatcgtctga agattgttct ccttcccaca
    34081 gtgagaaagg taattccccg gctacttcaa ccaaattagc agcaatccgt ttttggctaa
    34141 aataatctga aacaggcact tttcctgtag gtatttcccc gtctgctaac aagtaatgga
    34201 gtggagacca gccggaaatc tctttcggca acactaagtt aagctgagat tccgcgcggg
    34261 ttaatgccac atacagcaaa cgtaaatctt ccgcataagc ctcatactgt agcgaaaaac
    34321 ttgcctcttc tgagcctaaa caccaatcca gcgcaccttg agaattgcga tataaactta
    34381 atgctgtctg cgtgtctccc tcactttttt gtgaaacaaa aggtaaccaa acaattggat
    34441 attgcaagcc tttcgagccg tgaatcgtca caattttgat tagctcctct tcactttcca
    34501 accgaagttg ttgctcgtca ttaaaatcgg gcgaattaac ttgcttttca taccaccgca
    34561 acagttcagc ttcagactct aacgcttgcg aggtttcctg caaaatttcg gttaaatgca
    34621 ataaatccgt aatcaagcga tccgcatttt ccaaaccttt caaacgttcg ataatgccct
    34681 gctctaagta gattttatgc aacatcggca gaacgccctg ctctttccaa atgttgctgt
    34741 attccacaaa ctgattgact ttcagatccc aaagttgctc attttcctta tagcggtgga
    34801 tctctgccgc cgttaagccc cacaaggttg agcctaacgc cgataataat gccttgtaat
    34861 gatacggatt cagtgcggct tgcaataacc aacacaattc tgttgcaatt tccgaaccaa
    34921 atacagaagc tgattcagct aaatagacgg atttgatatt acgttttgcc aacgcctttt
    34981 tgattcgctt agcttgtgaa ccttttcgga ctaaaatcgc aatatctttt gcagcaaagg
    35041 attgaaattc gccgtttttt tcaactccga atttgccttg agccatcgcc tcaagctgtt
    35101 gatgaatttg ataagcacac atttcagcca aatcatctaa tttactgttt tccgtgatgt
    35161 agcctacaaa atgcccttgc tcactaacta actgaccttt ttggctatca gcctccactg
    35221 ggtgaaagcc aatatcctca taaagaaacg gagagttatc atttgcaaaa gaaaacaaat
    35281 tattgaccgc ttgcaccact tcaggcaacg aacgccagtt tttgcccaat gtgaattgtt
    35341 catcagcctg ctttgctgct cgcaaatacg tgaaaatatc cgccccacgg aatttataga
    35401 tcgactgttt cgggtcgcca atcataataa agccgttctc tgcattctct gccataaaaa
    35461 tttggctgaa aatttcatat tgggttaaat cggtatcttg gaactcatcg atcatcgcaa
    35521 aaggatacat cgcccgaatt ttctgagcca aggcttcgcc cgtgtgtaga tttttcaacg
    35581 cttggtttag cattgtgagt aaatcatcaa aactgcgttg tgggtgcgat tgtttgtact
    35641 ctgccaattt tgctttgaga gcaattaaaa attgatactg caacattgct gacaagctgt
    35701 tttcaaaacg ttccttgtaa gcggtcaaaa aagcctgatt ttttgcaaga tgaggggaat
    35761 ctatcggctc acagctcttt ttcgtgccag ccgcaatttt ctctgtagtg aataaataaa
    35821 gccctgccgg gaaatggtta tgttgcgaat tagcccactg gttcatcact tccacaccgg
    35881 cttcaaaagt ggtcgctcga taccaacctc cgtgcagcgt aattttatgc tttaaatcgt
    35941 ggcggatagt ttcaaccagt tcatctgaat gcgttttcca ataggatttc acctcattaa
    36001 taaactgctg atattgagcc aaatgctcgg caatatcttg atttaaagag gcatttggtt
    36061 caggtaattc agtagataaa taacgcctta ccgcattgag ggcatattcc ggcgtaccaa
    36121 gttgttcggc aaccgcataa gtgatggcta aatcttgcgg atagaattgc tctcgccaga
    36181 cttcttcact taatttttta agtagatcac tttggtttgg ctgtaaatcc aagtcaaacc
    36241 gcacgcccga ctcaaacgca aactgaacca gcattttttg acaaaagccg tgaatggtaa
    36301 acacgctggc ggtatcaatt tcccgttcgg caatacttaa acgtaacaat gcttcgtgaa
    36361 tattcggaat gagcggataa atctgtgcca aaaaatcaag gttgtttcga taggattttg
    36421 tactttccac ttccaaacca tcagcttgat cttttaaaaa gtcggcacat tgctgaatat
    36481 ttttacggat acggtttttc aactcttcgg ttgctgcttt ggtaaatgtc accaccaaaa
    36541 tttgttccac cgttaacggg ttgcaaccaa tgccgagcaa taaacgcaaa tacaggttgg
    36601 caatggtaaa ggttttccct gtacctgccg atgcttcgat taaaacggat ttattcaaag
    36661 gtagttggat aggatttagg tttttcatca gcaaagttat cttctaaaag taaaaagcac
    36721 tgctatgttc catagcagag aatctgttat ttaatccgtt ctaattttgc aattaattgt
    36781 gcttttggca ccgcttgtat ttttcgcatt tgccataata acaagccacc agccaaactt
    36841 aagccgatac aaaagactaa ccaaaaacct tgagcagcca ttggttgtac aatccaatcg
    36901 gttcttgcca aaatgtagcc cagcggcata ctcacccccc aataacaaaa cagagtaacg
    36961 tataaaatcg gttgggtatg tttataacca cgcagaatgc cgtttgctac cgcttgaatg
    37021 gcatccggta tttgataaac cgccgcaaaa atcaataaat gagcggtaat cgcaatcgaa
    37081 atcgggttgt tggtaaacgc aaaagggata aaatttttca acaaaataat aatgatggcg
    37141 gcaataatag ctaaagcggt tgcggt
//
LOCUS       DANJWC010000005        36509 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.005, whole genome shotgun sequence.
ACCESSION   DANJWC010000005 DANJWC010000000
VERSION     DANJWC010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 36509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36509
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.005"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..424
                     /locus_tag="PWX02_000149"
     CDS             <1..424
                     /locus_tag="PWX02_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HDL1728210.1"
                     /translation="KMTAKFLEVFDNHQCQIYSAPFDVRFPDENGKVKTVVQPDLCVI
                     CDVSKLDERGCVGAPDLVVEILSPGNTKREMKDKYELYQEQGVKEYWIVSPETRTIQI
                     FVLENGKYIGIQPVAEDEPATSVVFPALSFSTEKLFEL"
     gene            572..787
                     /gene="rpsU"
                     /locus_tag="PWX02_000150"
     CDS             572..787
                     /gene="rpsU"
                     /locus_tag="PWX02_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005701713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDL1728211.1"
                     /translation="MPVIKVRENESFDVALRRFKRSCEKAGILAEVRAREFYEKPTTI
                     RKREKASLAKRHAKRNFRENARNTRLY"
     gene            941..2689
                     /gene="dnaG"
                     /locus_tag="PWX02_000151"
     CDS             941..2689
                     /gene="dnaG"
                     /locus_tag="PWX02_000151"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL1728212.1"
                     /translation="MKGTIPRSFIDDLIARTDIVEVINSRVKLKKAGRDYQACCPFHH
                     EKSPSFSVSQSKQFYHCFGCGVHGNVITFLMDYDKLEFPEAIEELAAMHGLEVPRENV
                     IQRDGKPQANFKTKRNLYELMEAVAKFYQQNLSHQIEPQSYLQQRGLSPEIIERFEIG
                     FAPNAFDAALRTFGQSQEDIQKLLDTGVLTKNDSGRIYDKFRNRVMFPIRDKRGRVIA
                     FGGRVLPNDEQGAKYMNSPETVIYHKGSELYGLYQALKVNETPDYLFVVEGYMDVVAL
                     AQFGVDNAVASLGTATTGEQIQQMFRCTEQIVCCYDGDRAGRDAAWRALENALPYLQD
                     GRQLKFIFLPDGEDPDTFVRSQGKEGFEQYVAQNAKPLNDFLFDSLLADVDLSSKEGK
                     SKLAALAVPLINRIPGEMLRVYLRNILGQKLGILDPTQIEALLPTKKAAEKPLHQAVK
                     MKRTPMRLLIALLIQNPYLVKFVPDVSALASLKDPGFTLLTDIVATCQQNAGISMGGL
                     LEHYREDPQYKIVEALANWDHLELPENIERTFIDTLDFLYAKLVELRKEELIAKDRTV
                     GLTDDEKKEYALLMFQ"
     gene            2948..4819
                     /gene="rpoD"
                     /locus_tag="PWX02_000152"
     CDS             2948..4819
                     /gene="rpoD"
                     /locus_tag="PWX02_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDL1728213.1"
                     /translation="MENQADQQLEQQSQLELLISQGREQGYLTLSEVHDHLPEELVDA
                     EQIEDIIQMINDMGIQVLEAAPDTDDLLLAENIADEDVVEEATKVLSTVESELGRTTD
                     PVRMYMREMGTVELLTREGEIDIAKRIEEGINEVQCAIAEYPEALSGLLACYAQVETG
                     EMRLSDLVTAFVDPNILEEAPLEDIEAALGEDEESSDVPDVDDENEDEDGDSSSDSSD
                     DSSIDPELAREKFEALRSQHEKVLLVIQKHGRTSKKSKEQIEILSHIFREFRLVSKQF
                     DLLVASMQKLMKQVRTEERQIQRYAVEYAGMKKADFLKAFQGHELSEAWIEKAINAKK
                     NAVPKLANYVDNIRVNIANLQNLEQTSGLTIAQIREIGGRISNGELKARRAKKEMVEA
                     NLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAIT
                     RSIADQARTIRIPVHMIETINKLNRISRQCLQEMGREATPEELAERMGMPEDKIRKVL
                     KIAKDPISMETPIGDDDDSHLGDFIQDESLELPLDSATAESLRMATNEVLEGLTPREA
                     KVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSETLRSFLDD"
     gene            4913..5899
                     /locus_tag="PWX02_000153"
     CDS             4913..5899
                     /locus_tag="PWX02_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDL1728214.1"
                     /translation="MKKSLLALLIGSVFLISACEDGKMTQTVLEAEKQIVQLGTELKN
                     AQADLAKKEAEVAELSGVKAENEKLKSELENAQQNTALNVEIVKIFDKKEVIKHQVDP
                     KQEFAIEESTVSAFVSLPKTNFDWLNQLLINQAYDYNEEQGRKLKNPTEEEFKKHREA
                     VYQGLVESVKTEPAIGYDDHISSAFIGQRNNIATFTMMHYTYMGGAHGMHYTQYINVD
                     LAKKAIIGLNDLISPKNQVQLKDILWENYSSVRVDENGNYNGFADKKEFRISDQFYFT
                     PHGIAFVYPPYELGAYAEGDIEVEADWYIINKLLNSEYQRGEKDGFFEELDN"
     gene            complement(5975..6883)
                     /locus_tag="PWX02_000154"
     CDS             complement(5975..6883)
                     /locus_tag="PWX02_000154"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDL1728215.1"
                     /translation="MNKNSLFSIWFTTARPKTLPLALASIIVGSALAHWAGKFDVVTT
                     LLAFITTILLQILSNFANDYGDHVKGSDTKERIGPLRAIQHGVISGEQLKKAVIILSL
                     LSFVSGVALSAYAFETVQDLFFFIGLGIVSIIAAITYTVGKKAYGYLGLGDLFVLIFF
                     GFVAVIGVFYLQAHSLPIMILLPAFGCGLLSVAVLNINNLRDINQDRQAGKNTLIVRI
                     GSQNGRKYHVALLVLAVISYLIFAISEFEHWYSFIFLLSVPLLAKHARFVYQHKDPKE
                     LYPILGQMAGLALLTNLLFSLGIVLG"
     gene            7063..7800
                     /gene="tsaA"
                     /locus_tag="PWX02_000155"
     CDS             7063..7800
                     /gene="tsaA"
                     /locus_tag="PWX02_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HDL1728216.1"
                     /translation="MNSTSNQLTPIQLNPIGFVESPYDEKFSVPRQPNLVAEGKGILR
                     LIPPYNTPDAVRGLEKFSHIWLIFQFHHIPEREWHATVRPPRLGGNERVGVFASRATH
                     RPNPLGLSKVKLEAVVAGGSEVYLKLGSVDLVNGTPIFDIKPYIAFADSEPQAVSGFA
                     QQKPQARLAVRFSEEAKQAVEFCRNFAKFGIEQPLSFLADVIAQDPRPAYQQGKPSER
                     VYGMNLAGYNIRWQIENDTQAIVLSIE"
     gene            7858..8577
                     /locus_tag="PWX02_000156"
     CDS             7858..8577
                     /locus_tag="PWX02_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253715.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HDL1728217.1"
                     /translation="MKLKNFLTILPLAVATAVTSAETHTVEHKDKPASFNFSTEVSRT
                     VEKDVMQASVYSQKSGKSLPELKKFVSENLNRVLAEAKKHPSIEVQAEGVTNHVNYNN
                     KGNIDGWDTSGSIQLKSKDFDAMAAVLENLGKDVAIRYIDFSVSPEKMASLEDEMTLE
                     IISKFQHKADVIQKALGAKTYKLVNINIQTPNDGGRPYYEHRAMYAVSSMAQSKSMDE
                     IPLEAGKTTISATASGSVEFE"
     gene            8772..9458
                     /gene="aqpZ"
                     /locus_tag="PWX02_000157"
     CDS             8772..9458
                     /gene="aqpZ"
                     /locus_tag="PWX02_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HDL1728218.1"
                     /translation="MKKYFAEFFGTFWLVFGGCGSAVLAAGIPELGIGYAGVSLAFGL
                     TVLTMAYAVGHVSGGHFKPAVSIGLLVGGRFNAKDLVPYIIAQVIGAIAAGAVLYTIA
                     SGVPTFDATAGFAGNGYGEHSPHGYSLTAALLIEVVLTAFFLIIIMGATDKRAPAGFA
                     PIAIGLALTLIHLISIPVTNTSVNPARSTGVALFQGSWAIEQLWLFWVAPIVGAIIGA
                     VVYRFIAEEK"
     gene            9530..9670
                     /locus_tag="PWX02_000158"
     CDS             9530..9670
                     /locus_tag="PWX02_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728219.1"
                     /translation="MKKLIKVCGLISATIFLSGCLVTSVVGGVVGTAVDVVDTVTPDI
                     ID"
     gene            9773..10366
                     /gene="slyD"
                     /locus_tag="PWX02_000159"
     CDS             9773..10366
                     /gene="slyD"
                     /locus_tag="PWX02_000159"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL1728220.1"
                     /translation="MNITKDLVPSIAYQVRTQEGVLVDEAPVNQPLEYLHGHNNLVEG
                     LEKALEGKAVGDVFEVRVKPEEGYGEYNDNMVQRVPKDVFMGVDELEVGMRFIADTDL
                     GPLPVVITEVDGDEVVVDGNHMLAGQELLFSVEVVGIREATAEEIAHGHVHGGHEHGC
                     GCGGHDHHHDHDGCCGGHDHDHHHHHGHGGCGCGGHH"
     gene            10627..12159
                     /gene="ilvA"
                     /locus_tag="PWX02_000160"
     CDS             10627..12159
                     /gene="ilvA"
                     /locus_tag="PWX02_000160"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HDL1728221.1"
                     /translation="MMNETQHSQSAIDYLRSILSSNIYDLAQVTPLQKMEKLSERLGN
                     SIFIKREDRQPVHSFKLRGAFAMISNLSKAQKEAGVIAASAGNHAQGVALSAKHLGLR
                     ALIVMPQNTPAIKVDAVRGYGGEVLLYGANFDEAKAKAIELSTELGMTFIHPFDSPAV
                     IAGQGTIGLELVQQKPDLDYVFIPVGGGGLIAGIAVLIKQVMPNIKVIGVESKDSACL
                     YHALKQGQPTDLERVGLFADGIAVKRIGDETFRLCQQYVDDVVLVDNDEICASMKLIF
                     ENVRAISEPSGATSLAGLKKYVKEHQLQDKNLACILSGANVNFHTLRFVSERCEIGEK
                     REALLAVTIAEQKGSFLKFCEILGNRAVTEFNYRHSDDKQACIFVGVRISGSAEKVEI
                     IKDLQQNGYDVADLSDDDIAKTHIRYMVGGRASSIQYEQLYSFEFPEQKGALLKFLQM
                     LTNWDISLFHYRAHGADYGDILAAFALNRGDEVELNRHLEQLGYRFQNVSESPAYQYF
                     LK"
     gene            12185..13519
                     /gene="glmM"
                     /locus_tag="PWX02_000161"
     CDS             12185..13519
                     /gene="glmM"
                     /locus_tag="PWX02_000161"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDL1728222.1"
                     /translation="MARKYFGTDGVRGEVGKFPITPEFALKLGWAAGKVLATQGTKKV
                     LIGKDTRISGYMLESALEAGLTAAGLSAVFVGPMPTPAIAYLTRTFRAEAGIVISASH
                     NPYYDNGIKFFSSVGEKLPDEIEEAIEAMLDKPMDCVDSANLGRASRINDAAGRYIEF
                     CKGTFPSELSLEGYKIVVDCANGATYHIAPNVMRELGAEVLEIGTKPDGLNINEKCGA
                     TDIKALQKVVVEAGADVGLAYDGDGDRLIMVDHLGNKVDGDQILFILAREALRSGKLQ
                     GGVVGTLMSNMSLELALKELAIPFSRANVGDRYVLEQLKEKGWKLGGENSGHIIVLDK
                     NTTGDGIIASLQVLAAMASHKLSLNDLAKAVPLFPQVLLNVRFEKGAANPLESNEVKA
                     VAADVEKRLAGKGRILLRKSGTEPLIRVMVECEDAVLAQSCAEEIVEAVKRN"
     gene            13664..14323
                     /gene="rpiA"
                     /locus_tag="PWX02_000162"
     CDS             13664..14323
                     /gene="rpiA"
                     /locus_tag="PWX02_000162"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDL1728223.1"
                     /translation="MDQQEMKKIAAQAALKFVKSDTIVGVGSGSTVNCFIDALASMKD
                     EIKGAVAASKASEQRLRDIGIEVFSANEVTELDVYIDGADEITPQGAMIKGGGAALTR
                     EKIVSSLAKKFICIVDKSKQVDVLGSTFPLPVEVIPMARSYVARQLVALGGSPEYREG
                     VVTDNGNVILDVHNFKIMEPLKMEHTINNIAGVVTNGIFAQRFANVTIVGTPEGAVIL
                     E"
     gene            14360..15589
                     /gene="serA"
                     /locus_tag="PWX02_000163"
     CDS             14360..15589
                     /gene="serA"
                     /locus_tag="PWX02_000163"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDL1728224.1"
                     /translation="MAKISLDKSKIKFLLLEGVHQNAIDVLLAAGYTNIEVHKKALDG
                     QELIDAIKDAHFVGIRSRTFLTEEVLAQAPKLIAIGCFCIGTNQVDLNAAKRRGIPVF
                     NAPFSNTRSVAELVLAEMILLMRKVPTANAEVHRGVWNKSAAGANEVRGKKLGIVGYG
                     HIGSQLSILAEAIGMQVYFYDVENKLPLGNAQQVPTLEHLLSTCDMISLHVPEIDSTK
                     NLMSAERIAQLKEGSVLINAARGTVVDLDALAARIEDGSLRGAAIDVFPEEPASINDP
                     FVSPLCKFDNVILTPHIGGSTSEAQANIGTEVANKFVKYSDNGSTVTAVNFPEVSLPL
                     HTNTKRLLHIHENRPGMLNQINQIFVESNVNIAAQYLQTDAAIGYVVIDVESDNVDEA
                     LQRLKAIEGTIRARVLY"
     gene            complement(15667..16185)
                     /locus_tag="PWX02_000164"
     CDS             complement(15667..16185)
                     /locus_tag="PWX02_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HDL1728225.1"
                     /translation="MKKLTIVALTCLFSTHLLAAPVGNTFTGIGVGVDVTTTKYKVEG
                     VKGKDSTGPALVVDYGIEQGNNFVGVVQGKAKVGSTKVFSDVKQKNKYTIAYQQGYRV
                     GSDLLPYVKVDASSSKIGNETYRGFGFGAGAKYAVSSDVELGAEYTRDNLKRSGTKLK
                     GNAFTANATYRF"
     gene            complement(16205..16927)
                     /gene="arcA"
                     /locus_tag="PWX02_000165"
     CDS             complement(16205..16927)
                     /gene="arcA"
                     /locus_tag="PWX02_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HDL1728226.1"
                     /translation="MKIPQILIVEDELVTRNMLKSIFEGEGYQVFQASNGEEMHSVLS
                     TNEINLVLMDINLPGKNGLILAREIRENMKLPLMFLTGRDNEIDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLQRTMIDKENDVVKEQSEIESYRFNGWILDINSHSLISPEGEV
                     NKLPRSEFRALLHFCQHPGKIQTREDLLKKMTGRELKPHDRTVDVTIRRIRKHFEDHP
                     NTPEIIVTIHGEGYRFCGNIEP"
     gene            complement(17060..17752)
                     /locus_tag="PWX02_000166"
     CDS             complement(17060..17752)
                     /locus_tag="PWX02_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL1728227.1"
                     /translation="MILFAGDPHGSYEHLYPILQQQTEEVALVILGDLQLTTPDELDR
                     LSQYCDIWFIHGNHDSKTVAAFDSIWNSSWKERNIHGKVVDIQGIKIAGIGGVFRGHI
                     WMPPNRPMFFDPIHFCQYSAPEKIWRGGIPLRHRSSIFPSDFEALENEKADILITHEA
                     PKPHPQGFSVINQLARKLGASKIFHGHYHDNFNYGNLNVSRECAIYNIGFRSLADING
                     NYLILGVDDREK"
     gene            complement(17770..19044)
                     /gene="nadR"
                     /locus_tag="PWX02_000167"
     CDS             complement(17770..19044)
                     /gene="nadR"
                     /locus_tag="PWX02_000167"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HDL1728228.1"
                     /translation="MTDFAYLQQKRKQLKLKVNDICEQAGVTRAYFNQLVSGKIKNPS
                     ANKLKALHSVLDIVEDSNQRSGVIFGKFYPIHTGHINMIYEAFSKVDVLHVIVCTDSE
                     RDLRLFQESKMKRMPTNEDRLRWVQQIFKYQQKQIFIHHLVEDGIPSYPNGWEDWSGR
                     VKALFEEKSINPTIVFSSEIQDKEPYEKYLHLEVQLVDPTRESFNISATQIRNNPFQY
                     WRFIPKEVRPFFVKTIAILGGESSGKSVLVSKLANVFNTTSAWEYGREFVFEQLGGNE
                     QAMQYSDYPQMVLGHQRYVDYAMKHAHKVAIIDTDYITTQAFCIQYEGKAHPFLDSMI
                     REYPFDVTILLSNNTKWVADGLRSLGSKKQRQQFQHLLKKLLEKHHIPYIKIESPSYL
                     DRYNQAKEVVEAILNEEPIPLQFKQYNIHEFE"
     gene            19268..20230
                     /gene="ribF"
                     /locus_tag="PWX02_000168"
     CDS             19268..20230
                     /gene="ribF"
                     /locus_tag="PWX02_000168"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDL1728229.1"
                     /translation="MQLIRGFHNLDKHIALNQGCVLSIGNFDGVHLGHQNILARLCDR
                     ALDLGLPSVVMLFEPQPREFFAKKSGNSTACNPPARLMRLRDKLKYLEEAGVDFVLCV
                     RFSEKFAQISADDFISDLLAKRLKVRYLSVGDDFRFGAKRAGSFETLRNAGLKYRFAV
                     EESHTHSLNEERISSSLVREALQNNELDLAEKLLGKPYSILGRVAHGNKLGRTIGFPT
                     ANIMLNRLVTPIQEVFVVKVKTKQGSFNGIANVGNRPTINGTKPLLEVHIFDFNASIY
                     GEAIEVIFLKKLRNEIKFPDFEALKAQIEKDRLQAVEFFKEFAR"
     gene            20333..23149
                     /gene="ileS"
                     /locus_tag="PWX02_000169"
     CDS             20333..23149
                     /gene="ileS"
                     /locus_tag="PWX02_000169"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDL1728230.1"
                     /translation="MTIDYKNTLNLPETGFPMRGDLAKREPDMLKNWYDKNLYQKIRT
                     ASKGKKTFILHDGPPYANGNIHLGHAVNKILKDMILKSKTALGFDTPYVPGWDCHGLP
                     IELKVEGIVGKPNEKISAAEFRQACRDYAKEQVEGQKADFIRMGILGDWDNPYLTMNF
                     NTEANIIRTLGKVIANGHLYKGSKPVHWCLDCGSSLAEAEVEYEDKVSPSIYVRFNAV
                     DVNAVEEKFNAQGKGRGALSAVIWTTTPWTIPSNRAIAINPELDYALVQLGDERVLLA
                     VDLVEDVAKAAGVESAEILATTKGENLELLRFNHPFYDYSVPFIFGDHVTTDGGTGLV
                     HTAPDHGADDFVVARKYNIEMAGLISNDGKFKADTPFFAGLGVFESNDKVVEKLQEVG
                     ALLKLSRIKHSYPHCWRHKTPIIFRATPQWFIGMETQGLRQQALGEIKSVRWIPSWGE
                     ARIDTMVANRPDWCISRQRTWGVPMAMFVHNETEELHPRTLELIEEVAKRVEEKGIQA
                     WWDLDPAELLGEEAKDYRKVPDTLDVWFDSGSTYASVVEQRPEFNGNLADMYLEGSDQ
                     HRGWFMSSLMLSTATNGKAPYKQVLTHGFTVDEKGRKMSKSLGNVIVPSEVWNKNGAD
                     ILRLWVASTDYTGEITVSHNILNSAGESYRRIRNTARFLLANLNGFDPKSDLVQPQEM
                     IALDRWAVACALEAQNDIKEAYDNYQFHTVVQRLMRFCSIEMGSFYLDIIKDRQYTTK
                     ADSLARRSCQTALWHIAEALVRWIAPILSFTADEIWGYLPQVEGRSEFVFTEEFYTGL
                     FGLTEQDKLDDAYWQQILKVRAEVNRVLEQARKDKVIGAGLEAKVTVFANDEIRPLLE
                     QLGNELRFVLITSQAIVKPLAEADIAEGELAGLAVKVENADGEKCPRCWHYATDIGSH
                     SGHEEVCGRCVENVAGEGEKRLFA"
     gene            23274..24821
                     /locus_tag="PWX02_000170"
     CDS             23274..24821
                     /locus_tag="PWX02_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HDL1728231.1"
                     /translation="MMKIIAFLFIFLSVFSHTALANSMAEQCVALKSNSPEKTAIYKA
                     EWVEKSAELPAYCLVQGEIGKRVGVDNVPYGIQFELRLPEQWNEKFLFQGAGGVGGVI
                     FPAIGKLYPHGASGANGLNRGYAVVSNDSGHATRDLVFTADPEARSNYAYASIGKVTT
                     VAKQLIAEFYRKPSKHNYIMGCSNGGREAMMAAMRYPQEFDGVIAGSPGFRVSRSVLA
                     EVWDNRALLAVAPKNGDGDKILSQALTQQDLDVIANGVLTRCDKLDGLADGLINAWEQ
                     CDFQPEMVAKQLGQKKVALIKTIFEGAKNSRGELIYSPWAYDSGINAKGWRDWKLGDS
                     QTAQPNSISFKMGLKSLTHYYLAPRVPSRDPLSVDLDQASEQVKAVGSIHDADQVDLS
                     AFKQNGGKMLIYQGVSDPIFSAVDLKNWYQSLQQAVENPQHFAKLFFVSAMNHCGRGA
                     TVNDFDMLTALENWVEKGLEPESIAAKAGELYPNKAKQIPLCAYPKVAIYQTGEDPNR
                     LTSYQCR"
     gene            24891..26258
                     /gene="pssA"
                     /locus_tag="PWX02_000171"
     CDS             24891..26258
                     /gene="pssA"
                     /locus_tag="PWX02_000171"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HDL1728232.1"
                     /translation="MLILNKQRKAEYLLKSLPYLPQVAEQVQFLASSKAFKQQILQLI
                     QTAKKRIYITALYFEKDEAGQEVLAALYQAKRNNPELEINILVDWHRAQRGRIGEAEN
                     LSNADWYAKVKYEQGLPAGQEIAFYGVSINGREVFGVLHLKGFVFDDTILYSGASINN
                     VYLHQFERYRYDRYHVIENKVLADSWVTFIQQHLLTNEAVKRLDTENRPKTAEIRAQI
                     KHFRKQLSREAYLFNGVAENGRLAVSPLVGLGRRKNQLNKVIEALFYQTEQKLTICTP
                     YFNFPHSLRTRIEWLLANGKSVEIIVGDKTANDFYTKPDEKFTMASALPYLYEKNLRA
                     FAKRLDGYVQNKQLTIRLWKDGENSYHLKGVWVDNRYILLTGNNLNPRAWRLDAENAI
                     LISDPQHQLSEKAETELNQICQHTQILTHYSDLEVLTDYPENVRKLLKKFGRVKLDKI
                     VKMLL"
     gene            26313..27056
                     /gene="rlmB"
                     /locus_tag="PWX02_000172"
     CDS             26313..27056
                     /gene="rlmB"
                     /locus_tag="PWX02_000172"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDL1728233.1"
                     /translation="MSEQIYGIHAVKAFWDNAPERLIEVLVLKGREDKRLMPLLNELQ
                     RLGVAIQQVNRQTLDNKSQGEVHQGIIARVVPQKELNEHDLEAILSQKKNPLLLILDG
                     VTDPHNLGACLRTADAAGVDAVIVPKDKSAQLTPTARKVACGAAEVMPLIRVTNLART
                     MRDLQERHNVWIVGTAGEAESGIYEAKLTGSIALVMGAEGDGMRRLTREHCDQLISIP
                     MAGSVSSLNVSVATGVCLFEIVRQKLAGQ"
     gene            complement(27143..28555)
                     /locus_tag="PWX02_000173"
     CDS             complement(27143..28555)
                     /locus_tag="PWX02_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1728234.1"
                     /translation="MKLLNIQNLSVETENRLQLVEPISLQLKAGKNITILGETGSGKS
                     LLIQAIMGALPDGLIASGEISVKNCKISTESDRLQTLWGKTLVMLPQEPRRSLNPIMA
                     IGKQLWESFHYVAGKPSSQAKTESKAYLAKLGLGESVENYPHQLSGGMAQRASFAIAT
                     AAGGKILLADEPTKGLDPTSKGKVIELLKSAYQNGGGLLTITHDIEVAEQLGGTILVM
                     KKGELLEQGEAKALLNNPQHPYTKALISADPKHWQPLQDLQNLSQKQPLVSVKNLSIA
                     RGKKTLFSNLSFDLHKGEVLGIVGHSGIGKSSLADVLCGLLKPKSGEVVWHSHSHKKH
                     QVLKLYQDPPEAFAPNVSLKTLLDDVINKHQLDRLPIPSLLQQLSLNPEILQRNAENV
                     SGGELQRVAILRALLLEPVLLFADEVTSRLDPITQQETIELLINQCRARNCSLILVSH
                     DPYLIEKSCDKVINLEEYVG"
     gene            complement(28644..29426)
                     /locus_tag="PWX02_000174"
     CDS             complement(28644..29426)
                     /locus_tag="PWX02_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1728235.1"
                     /translation="MKISISQYLGGTILLAMLGFAFLQPYFYPMDIGFQDLTNILSNP
                     NEIAWFGTDHLGRDMLARLASAIRLSFGLSLFSVICALIAGLITGIMAGFFGGWIDRI
                     FSFICDLVMALPGLLLVLLFAALSPGSFWTLYLGISLVMWVEFFRVIRSISSTMANSS
                     EIQASRLMGMGWFYSFKRHLLPRLLPMIATLSAFSLGNAILALATLGFVSVGLRPPTA
                     ELGLMMTELFPYYYEAPLIFLQPVVAVFLMVLSLQLLSRKIK"
     gene            complement(29436..30368)
                     /locus_tag="PWX02_000175"
     CDS             complement(29436..30368)
                     /locus_tag="PWX02_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1728236.1"
                     /translation="MHKILFRRLIQILLVIWSVGTLTFILTRQLSGDMAYRIAASRYG
                     YDQADSAAAEAVRAELGLDQPWWQSYFSWLSDLAQFNLGKSLVTGDPVWSEIQHQFGH
                     TLSLAIVALILAILIGPPLGLLAARKPNGFFDRLTLVFSTLFRSVPAFIIAIGLITLF
                     SAQLKWLPAGGYGSWQHFILPALTLAIGLSTVSVRVTRSAMVQVKGSEFYQFSQLKGL
                     SRWHTFLRHGLPNIAVPVIAYHAVQLVYLIEGVVVVESLFAWPGSGHALVHAIIARDV
                     PMIQGTTLVMGGLFVVLNTIADLLSAWIDPRIKR"
     gene            complement(30588..32162)
                     /locus_tag="PWX02_000176"
     CDS             complement(30588..32162)
                     /locus_tag="PWX02_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1728237.1"
                     /translation="MKKWLIPLTVAAFCVSACDDKNSTQATAEKPQVENTQSQKNAEI
                     LPLATVVAPWEINSLDPNQSGVIFQRMNLAETLVEADLNGNLIPALATDWRSNEEGTI
                     WRFTLREGVKFHNDQVLTAQAVVNSLNIAKSKPGVLAKAFIKEIRAVDNKVEIELTKA
                     FVPFPSFLTHYTALILAPESYNEKGEVTQIIGTGAFKPTKIEAPQKLEAVQFEGYWGK
                     KPQVQQANYLASSRSETRMLMAQSEPSSLVFNLDTASVARLKADPNLQLVSGSIARTI
                     QLKMDVAKPFFNDLAIRQALSQAINRKAIAEQVLKIENGAADQILPKAFADWRVDTAE
                     QVVDIAKIKQNLTASGYTFDEQGRLTKDGKPFSFTMRTFSDRPELPVIATILQAQWKQ
                     IGVEVNVSVGNFSEIPAGHQDGSLEMALYALNYGKTLDPYGVIASDFANGGSDWGVMN
                     WQNTEVDNAVKSLETERDPQKAKALKQTVSKIIYNELPIIPVVYYQQNVVAHKEVKNV
                     TLDPFERRFFLELLSK"
     gene            complement(32420..34000)
                     /gene="prfC"
                     /locus_tag="PWX02_000177"
     CDS             complement(32420..34000)
                     /gene="prfC"
                     /locus_tag="PWX02_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDL1728238.1"
                     /translation="MSYPQEVNKRRTFAIISHPDAGKTTITEKVLLYGNAIQTAGSVK
                     GKGSAQHAKSDWMEMEKQRGISITTSVMQFPYNDCLVNLLDTPGHEDFSEDTYRTLTA
                     VDSCLMVIDSAKGVEERTIKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVESVLK
                     IKCAPITWPIGCGKLFKGVYHLYKDETYLYQTGQGHTIQEVRVVKGLNNPELDQAVGD
                     DLAQQLRDELELVQGASHEFDHAEFIKGELTPVFFGTALGNFGVNHFLDGLTEWAPAP
                     QARQSDEREVSPSEEKFSGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLKHVRIG
                     KDVVISDALTFMAGDRSQAEEAYAGDIIGLHNHGTIQIGDTFTQGENFKFTGIPNFAP
                     ELFRRIRLRDPLKQKQLLKGLVQLSEEGAVQVFRPLINNDLIVGAVGVLQFDVVVSRL
                     KTEYGVEAIYETVNVATARWVEGKDAKKFEEFKRKNEANLALDGGDNLTYIAPTMVNL
                     NLAQERYPDVQFFKTREH"
     gene            34233..35108
                     /locus_tag="PWX02_000178"
     CDS             34233..35108
                     /locus_tag="PWX02_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDL1728239.1"
                     /translation="MMKKFIPVMIAALVLSACSSQPAKRKSTPRIKATAAYIPTENSA
                     YVVFDLNQKKFLEGKNFNLVRPIASVTKLMTAVVFLEENRYGASCQTQILPSDADFLK
                     GTTSPLPKNLNIACSELLKAMLVRSDNYAAHALAHATHLNKAQFLARMNSKAREIGMT
                     QTYFGDSSGLSSDNVSTVVDLAKLAGYAASKPEIQQLSNSPLVSVYAGGRYFSMKNTN
                     SMVRSQSYQALVSKTGYTREAGYNLAFIAKGDCRGKRIGVVSLNNDNAASRTDFTESM
                     LAKYQCVPNPYRQYD"
     gene            35181..35768
                     /gene="pth"
                     /locus_tag="PWX02_000179"
     CDS             35181..35768
                     /gene="pth"
                     /locus_tag="PWX02_000179"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDL1728240.1"
                     /translation="MSEIKLIVGLANPGVKYEETRHNAGEWLINELARIYNVSLKEEP
                     KYFGKVAKINTAQGEVRLLVPTTFMNLSGKAVGALANFYRIKPEEILVAHDELDLPPG
                     SVKLKKGGGHGGHNGLKDIIAALGNNKEFYRVRIGIGHPGHKDLVANYVLGKPSPSDQ
                     TLLNSAVDEAARCVEVLFKDGMVKATNRLNSFKAN"
     gene            35954..>36509
                     /gene="ychF"
                     /locus_tag="PWX02_000180"
     CDS             35954..>36509
                     /gene="ychF"
                     /locus_tag="PWX02_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDL1728241.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANYPFCTIEPNTGVV
                     PMPDPRLDALAEIVKPERVLPTTMEFVDIAGLVAGASKGEGLGNKFLANIRETDAIGH
                     VVRCFENDDIVHVAGKIDPADDIEIINTELALADLDSCERAIQRLQKRAKGGDKDAKF
                     ELSIMEKILPVLENAGMIRSVDLDK"
BASE COUNT        10717 a         7145 c         8330 g        10317 t
ORIGIN      
        1 gaagatgaca gcaaaatttt tagaggtttt tgacaatcac caatgccaga tttattctgc
       61 gccttttgac gttcggtttc ccgatgaaaa cggaaaagtc aaaactgtag tgcagcctga
      121 tctttgtgta atttgtgatg tcagtaagtt agatgaaaga ggctgtgtag gggcaccgga
      181 tttagtggtg gaaatccttt ctccgggtaa taccaaaagg gagatgaaag ataaatacga
      241 gctttaccag gaacaagggg tgaaagagta ttggattgtt tctcccgaaa cccgcactat
      301 tcagattttt gtgttggaaa acggtaaata tatcggtatt cagccggtgg cagaagatga
      361 accggcaact tcggtggtct ttccggcatt aagtttttcg accgagaaat tattcgaact
      421 gtagaaaaat gcaacataat acggcggttt tatgccacaa tactacttta cttatgtgag
      481 tgcttaaggt agaatgcact accttttttg ttctatgccg ccaaaattgt gtgcggttta
      541 atttaatcat atttttctga ggtgatggca tatgccagta attaaagttc gtgaaaatga
      601 atcatttgac gtagcattac gtcgtttcaa acgctcttgc gaaaaagcgg gtatcttagc
      661 tgaagttcgt gctcgtgaat tctacgaaaa accaactaca attcgtaaac gtgaaaaagc
      721 atcacttgct aaacgtcacg ctaaacgtaa tttccgtgag aacgcacgta acacacgttt
      781 atactaatta gttaggctgg ttagcaaact atatcgaaac cgtgattccg ttaggctcac
      841 ggttttggtg tttttaacgt ttctatttca agcggttgtt ttttgcaaaa aatttgcaaa
      901 attgctccgc ttgtatcatt caaagagcag gaggaaatca atgaaaggca caattccacg
      961 ttcatttatt gatgatctta tcgctcgcac cgatattgtt gaggtcatca acagtcgggt
     1021 caaactcaaa aaggctgggc gagattatca agcctgctgt ccttttcatc acgagaaaag
     1081 cccttccttt tcggtaagcc aatcaaaaca gttttaccat tgtttcggct gtggcgtaca
     1141 cggcaatgtc atcacctttt tgatggatta tgacaaactc gaatttccgg aagcgattga
     1201 agaactggca gctatgcacg ggctggaagt gccgagagag aatgttattc aacgcgacgg
     1261 caagcctcaa gccaatttta aaaccaagcg taatctttat gaattgatgg aggcggtggc
     1321 gaagttctat cagcagaatt tatcgcacca aattgagcca caaagctatt tacagcaaag
     1381 aggtttgtcg cctgagatta tcgaacgctt tgaaatcggt tttgctccga atgcctttga
     1441 tgcagccctc cgtacttttg ggcaatcgca ggaagatatt cagaaattgc tcgatacggg
     1501 cgttttaacc aaaaatgata gcggtcgaat ttacgataaa tttcgcaatc gggtaatgtt
     1561 cccgattcgg gataagcgtg gacgagtgat tgcctttggt gggcgagtgc tgccgaatga
     1621 tgagcaagga gcgaaatata tgaactcgcc cgaaacggtg atttatcata aaggtagcga
     1681 gttatatggc ttgtatcaag cactgaaagt caatgaaacg ccggattatc tattcgtggt
     1741 ggaaggatat atggacgtgg ttgcccttgc tcaatttggg gtggataacg cagttgcttc
     1801 tttaggcacg gcaaccactg gtgaacaaat tcagcaaatg tttcgttgca ccgagcaaat
     1861 tgtctgttgt tatgatggcg atagagccgg ccgagatgct gcatggcgtg cattagaaaa
     1921 tgcgttgcct tatttgcaag acggtcgcca actgaaattt attttcttgc ccgatggcga
     1981 agatccagac acctttgttc gttctcaagg taaagaagga tttgagcaat atgtggcaca
     2041 gaatgccaag ccattaaacg atttcttatt tgacagtttg ctggcggatg tggatctctc
     2101 ttcaaaagag ggaaaatcca agctcgctgc tttagctgtg ccgttaatta atcgtattcc
     2161 gggcgagatg ctgcgagtgt atttacgcaa tattttagga cagaaattag gcattttaga
     2221 cccaacgcag atagaagctc tgttaccgac gaaaaaagcg gcagagaagc ctttacatca
     2281 agcggtcaaa atgaaacgta cgccaatgcg gctgttgatt gcgttattga ttcaaaatcc
     2341 gtatttggtg aaattcgtac ccgatgtaag tgctttagcc tccttgaaag atcctggctt
     2401 tacattatta accgatattg tcgcaacttg tcagcaaaat gcaggtatca gtatgggcgg
     2461 gctacttgaa cattatcggg aagatcccca atataagatt gttgaagccc tcgcaaattg
     2521 ggatcattta gagttacccg aaaatatcga acgaacattt atagacactc tcgattttct
     2581 ctacgccaaa ttagtggaat tacgcaaaga agagctaatt gccaaagacc gaactgtggg
     2641 cttaaccgat gatgagaaaa aagagtatgc tcttttgatg tttcaataga attttaagct
     2701 cgaataattc ccctctttag caaagagggg ctaggggaga tttggcagaa gtgatgaacg
     2761 aagaaaataa ttaattctat ctaaaaagaa agttatttat ctctcttgct acacctctct
     2821 gttctcataa gcatcaaagt aatgtgtaaa ttagaaaaat agaatttttt tgtgaattta
     2881 tgaagtcgaa ccaaatttag tggtataatc cactactatt tttgctcgca taataaacgg
     2941 atagactatg gaaaaccaag cagatcaaca attagaacag caatctcaat tagaacttct
     3001 gatcagtcaa gggcgtgaac agggatattt aacgctttct gaagtgcacg accacttacc
     3061 ggaagagttg gtggatgcgg agcagatcga agatattatt cagatgatca acgatatggg
     3121 tatccaagtt ttagaggctg cccctgatac ggatgattta ctactcgctg aaaatatcgc
     3181 cgatgaagat gtggtagaag aagccaccaa agtgctttca accgtagaat cagagttagg
     3241 tagaactact gaccctgtgc gtatgtatat gcgtgagatg gggacagttg agcttttaac
     3301 ccgtgaaggt gagattgata tcgctaaacg cattgaagaa gggattaacg aagttcaatg
     3361 tgcgattgcg gaatatccgg aagccttaag tggcctgtta gcgtgctatg ctcaagttga
     3421 aaccggtgaa atgcgccttt ctgatttagt gacagcattt gtcgatccta atattttaga
     3481 agaagcaccg ttagaggata ttgaggctgc tttaggtgaa gatgaagaat cttctgatgt
     3541 gcctgatgta gatgacgaaa acgaagatga agacggtgat agcagctcag acagcagtga
     3601 tgacagctca attgacccgg aacttgcccg tgaaaaattt gaagccctac gcagccaaca
     3661 tgaaaaagtg ttattggtca ttcaaaaaca cggacgaacc agtaaaaaat cgaaagagca
     3721 aattgaaatt ttatctcata ttttccgtga gttccgtttg gtgtcgaagc aatttgactt
     3781 attggttgct tctatgcaaa aactgatgaa gcaggttcgc actgaagaac gtcaaattca
     3841 acgctatgca gtggaatatg ccggcatgaa aaaagcggat ttcttgaaag cattccaagg
     3901 gcacgaatta agcgaagcgt ggattgaaaa agcgattaat gccaagaaaa atgcagtacc
     3961 gaaattagct aattatgtgg ataatatccg tgtcaatatt gctaacttac agaatttaga
     4021 gcaaacatca ggcttaacca ttgctcaaat tcgtgaaatt ggcgggcgta tttccaatgg
     4081 tgagttaaaa gcccgccgtg cgaaaaaaga gatggtggaa gctaacttac gtttggtgat
     4141 ttcgattgcg aaaaaataca ctaaccgtgg cttgcagttc ttagatttaa tccaagaagg
     4201 aaacatcggt ttaatgaaag cggtggataa atttgaatac cgccgtggtt ataaattctc
     4261 gacttatgca acttggtgga ttcgtcaagc gattacccgt tcgattgctg accaagcccg
     4321 tacaattcgt attccggtgc atatgattga aactatcaac aaactcaacc gtatttcacg
     4381 ccaatgttta caagaaatgg ggcgtgaggc aacgccggaa gaactggcag aacgtatggg
     4441 aatgccggaa gataaaatcc gcaaagtatt gaaaattgcc aaagatccga tttcaatgga
     4501 aacaccaatc ggtgacgatg acgattcaca tttaggtgat ttcattcaag acgaaagcct
     4561 tgaattacca ttggattccg ccactgcgga gagcctgcgt atggcaacga acgaagtgtt
     4621 agagggctta accccgcgtg aagcgaaagt gttgcgtatg cgttttggta ttgatatgaa
     4681 tacagaccac acactcgaag aagttggtaa acaatttgac gtaactcgtg aacgtatccg
     4741 tcaaattgaa gcgaaagcac tgcgcaaact tcgccacccg agccgttcgg aaaccttacg
     4801 cagtttctta gacgactaat catttcccct atgcctgatg gtatagggga ttttgtttta
     4861 atctgtataa caagcggtga tatttaccgt attttttaca aaagagggag agatgaaaaa
     4921 atcactatta gcacttttaa ttggctcagt atttctgatt tcagcttgtg aagacggcaa
     4981 aatgacccaa acggtattag aagccgaaaa acaaattgtt cagttaggca cagaattaaa
     5041 aaatgctcag gctgatttag caaaaaaaga ggctgaagtt gcagaacttt caggggtgaa
     5101 agcagaaaat gaaaaattaa aatcagagtt agagaacgct cagcaaaaca cagcgttaaa
     5161 tgttgaaatt gtgaaaatct ttgataagaa agaggtgatt aagcaccaag ttgatccgaa
     5221 acaagaattt gcaatagaag aaagtacagt aagtgcgttt gtgtctctac caaaaaccaa
     5281 ttttgattgg cttaaccagc ttttgattaa tcaggcctat gattacaatg aagaacaagg
     5341 cagaaagctg aaaaatccaa ccgaagaaga gtttaaaaag catcgtgaag cggtttatca
     5401 agggttggta gaaagtgtga aaaccgagcc agctattggc tatgatgatc atatttccag
     5461 tgcttttatc gggcaacgca ataatattgc tacattcact atgatgcatt acacctatat
     5521 gggtggggct cacggtatgc actatacgca atatatcaat gtagatttag ctaaaaaagc
     5581 aatcattggc ttaaatgatt taatcagccc aaaaaatcaa gttcaactga aagacattct
     5641 ttgggaaaat tacagtagcg tgcgtgtgga tgaaaatggc aactataatg gatttgctga
     5701 taaaaaagag ttccgtattt ctgaccaatt ctactttacc cctcacggca ttgcttttgt
     5761 ttatccgcca tacgaattag gtgcttatgc agaaggcgat atagaggttg aagcggattg
     5821 gtacattatt aataagttgc tcaattcgga gtatcaacgt ggcgaaaaag atggattctt
     5881 tgaagaacta gataactaat tgtaaattta gataaaaaac aaccgcttgt aatcctttat
     5941 tacaagcggt ttagttttgt taaaattttg caacttaccc taacacaatg cccaaactaa
     6001 ataatagatt agtgagcaac gccagccctg ccatttgccc tagaattggg tagagttctt
     6061 ttgggtcttt atgctgatag acaaaacgag cgtgttttgc cagtaacggt acagacagca
     6121 aaaaaatgaa gctataccaa tgttcaaatt cagaaattgc aaaaatcaga taagaaatga
     6181 ccgctagcac taacaatgca acgtggtatt ttcgcccgtt ttggctaccg attcgtacaa
     6241 tgagagtgtt tttcccagct tgtcggtctt ggttaatatc acgcaagtta ttgatattta
     6301 acactgctac agacagcaat ccgcaaccga aagcagggag cagaatcatt ataggtaggc
     6361 tatgagcctg caagtagaac acgccgatta ccgctacaaa gccaaagaaa atcagcacaa
     6421 atagatcgcc cagccccaaa tagccgtagg cttttttgcc gacggtgtaa gtaatggcgg
     6481 caataattga gacgatgcca agcccgataa agaaaaacag atcctgcact gtttcaaaag
     6541 cataggcact taaggcaaca ccggaaacaa acgagagtag gcttaagata atcacggctt
     6601 tcttaagttg ttcaccgcta ataacgccgt gttgaatcgc ccgtagcggt ccgattcgtt
     6661 ctttggtgtc agagcctttg acgtggtcgc cgtaatcatt ggcgaagtta gacaggatct
     6721 gcaacaaaat ggtggtgata aatgcgagta aggtggtaac aacatcaaat ttccctgccc
     6781 aatgagcaag tgctgaaccc acaataattg aagctaacgc cagcggtaaa gttttcgggc
     6841 gagcggtggt aaaccaaatc gaaaagagag aatttttgtt catagagaat aatcttagcg
     6901 taatttcatt tacaatgatg ttaaggattt aagcgatagt gcaagcgtcc actcttgtgt
     6961 taaatggtaa aacgaattgg tacaagcgtg gacgcttgca ccatcctcaa tttcatatca
     7021 ttatgtccaa tattttatat tattttaaca gaaaaaagcg taatgaactc tacctcaaat
     7081 caactaaccc cgattcaatt aaaccctatc ggttttgtgg aaagccccta tgatgaaaaa
     7141 ttttccgtgc ctcgtcagcc gaatttggta gcggagggga aaggcattct acgtttaatt
     7201 ccgccttaca atacccccga tgcagtcaga ggcttggaga aatttagtca tatttggttg
     7261 attttccaat tccatcacat tcccgaaaga gagtggcacg ccaccgtcag accgcctcgc
     7321 ttgggcggga acgaacgggt cggggtcttt gccagccgtg ccacccatcg tccgaatccg
     7381 cttggcttat cgaaagtgaa gttagaagcg gtagtggcgg gggggagtga agtttatttg
     7441 aaattaggca gcgtggattt agttaacggc acaccaattt ttgacatcaa gccttacatt
     7501 gcatttgcag atagcgagcc gcaagcggtt tccggttttg ctcagcaaaa accacaagca
     7561 agattagcgg tgcgttttag cgaggaggca aaacaagctg tcgaattttg ccgaaatttt
     7621 gcaaaatttg gcatcgaaca gccgcttagt tttcttgccg atgtcatcgc ccaagaccct
     7681 cgcccggcgt atcaacaagg gaaacctagt gagcgagttt atggtatgaa ccttgccggc
     7741 tacaatattc gctggcaaat tgaaaatgat acacaggcga ttgtgctttc gattgaatga
     7801 actttcctac gcaagtgaga tctaagaaat gatttttaac ccttttaagg actgattatg
     7861 aaattgaaaa attttcttac cattctaccg cttgcagtcg caacagcggt aacttcagcg
     7921 gaaactcata ccgtggaaca taaagataaa ccggcttctt ttaatttctc aaccgaagtc
     7981 tctcgcacgg tggaaaagga cgtgatgcag gcaagtgtgt atagccaaaa atcaggtaaa
     8041 tccttgccgg agctgaaaaa atttgtatct gaaaacctga atcgtgtatt ggctgaggca
     8101 aaaaaacatc catcgattga agtacaagct gaaggtgtga ctaatcacgt taattataac
     8161 aacaaaggga atattgatgg ttgggatacc tctggtagca ttcagttgaa aagcaaagat
     8221 tttgatgcaa tggcagcggt gttggaaaat ttaggtaaag atgtggcgat tcgttacatt
     8281 gattttagtg tatcgccaga gaaaatggcg tcccttgaag atgaaatgac gctggaaatt
     8341 atcagcaaat ttcagcataa agccgatgtg attcaaaaag ccttaggggc gaaaacatat
     8401 aagctagtta atatcaatat tcaaactcca aatgatggtg gtagaccata ttatgaacac
     8461 agagcaatgt atgcagtttc atcaatggca caaagcaaat ctatggatga aatcccgtta
     8521 gaagcaggta aaaccacaat ttcagctacc gcttcaggaa gtgtggaatt tgaatagaaa
     8581 tgtatagatt tgtatagagt tgtattttta ctttaattgg aggaaaaaat cggtctatac
     8641 tatcaattaa gaaatatgcg tctttacttt ttttagtata ctcatatttt attggctatg
     8701 ttttatagca ttcggaaagt taatacactt atgtgtgatt caccctttag ggtaaggata
     8761 aaagataaaa aatgaaaaaa tattttgctg agtttttcgg gacattttgg ttagtgtttg
     8821 gtggttgcgg cagtgcagtt ttagcagcag gcattcctga attaggaatc ggttatgcag
     8881 gcgtttcgtt agccttcggt ttaaccgtat taacaatggc gtatgcagtc ggtcatgttt
     8941 caggtggtca cttcaaaccg gcggtttcaa tcggtttatt agtcggcggt cgttttaacg
     9001 ctaaagattt agtgccttat atcattgccc aagtaatcgg tgcgattgct gccggtgcag
     9061 tgttatacac cattgcttca ggtgttccga cttttgatgc tacagcaggc tttgccggca
     9121 acggttatgg tgagcattcg ccacacggct atagcttaac agcagcatta ttaattgaag
     9181 tggtattaac ggcattcttc ttaatcatca ttatgggtgc aaccgataaa cgtgctccag
     9241 caggtttcgc tccgattgct attggtttgg ctttaacctt aatccactta atcagcattc
     9301 cggtaaccaa tacctcagta aacccggcac gttcaaccgg tgtagcgtta ttccaaggca
     9361 gctgggcgat tgagcaatta tggttgttct gggttgctcc gattgtcggt gcaattatcg
     9421 gtgcggtagt ttatcgcttt atcgctgaag aaaaataatt taagcgaata aatatgatgc
     9481 agctctagtc ttgattaggg ctgttttttg tttttgaaag gattttaaaa tgaaaaaatt
     9541 aatcaaagta tgtgggctga tttcagcaac tattttccta agtggttgtt tggtaacttc
     9601 ggtggttggt ggagtggtgg gaaccgcagt tgatgtggta gacaccgtaa cccctgatat
     9661 tatcgactaa tttactgaca agcggttaaa atttccagat agattgcaaa tataaaataa
     9721 agcgttacaa tggcttgcta ttttataaaa ccgatgaatt aaaggattca atatgaacat
     9781 tacaaaagat ttagtgccaa gcattgcata tcaagtgcgt actcaagaag gcgtattagt
     9841 agatgaagca ccggtaaacc aaccgttaga atatttacac ggtcacaaca acttggtaga
     9901 aggcttagaa aaagcccttg aaggcaaagc tgtaggcgat gtgtttgaag tgcgtgtgaa
     9961 accggaagaa ggttacggcg agtacaacga taatatggtt caacgtgtac cgaaagatgt
    10021 atttatgggc gtcgatgaat tagaagtggg tatgcgtttt attgcggaca cggatttagg
    10081 tccgctaccg gtggtaatta ccgaagtaga tggcgatgaa gttgtggtgg acggtaacca
    10141 tatgttagcc ggtcaggaat tacttttctc tgtggaagtg gttggcattc gtgaagcgac
    10201 agcggaagaa atcgcacacg gacacgttca cggcggacac gaacacggtt gcggttgtgg
    10261 tgggcacgat catcatcacg atcacgatgg ttgctgcggc ggtcacgacc acgatcatca
    10321 ccatcatcac ggacacggtg gctgtggttg cggtggtcat cactaacttt gtgatttccc
    10381 ctctccccgc ttgcgggaga gggtggagca aatcgaagat ttgcggaggg agagggggat
    10441 atccaaggtt tcccctctct ctgcttaaaa tttctgaacg aaattttaag ctgtctctct
    10501 cccgcaaacg ggcgagagga aatattccct gttaaagcca ttgctaaata accaaacaag
    10561 cggtcaattt tccttggaaa tttaccaatg tctttatctc aatctaacaa taaataacct
    10621 ttcaaaatga tgaacgaaac acaacattcc caatccgcta tcgactattt acgctcgatt
    10681 ttaagctcaa atatttatga cttagcgcaa gtgacccctt tacagaaaat ggaaaaactg
    10741 tctgagcggt taggcaactc tatttttatc aaacgtgaag accgacagcc ggttcacagc
    10801 tttaaattgc gaggtgcttt tgcgatgatc tcaaatttat ccaaagcaca aaaagaggct
    10861 ggagtcattg cggcttcagc aggcaaccac gcacaagggg tggcactttc tgccaaacat
    10921 ttaggtttaa gagccttgat tgtaatgccg caaaacacac cggcaattaa ggttgatgcg
    10981 gtacgtggct atggtggtga agtgttgctt tatggggcga attttgatga agctaaggcg
    11041 aaagcgattg agctttccac cgaattgggt atgaccttca ttcacccgtt tgacagcccg
    11101 gcagtgattg cagggcaagg cacaatcggc ttagaactgg tgcaacaaaa gccggattta
    11161 gattatgtat ttattccagt gggcggtggc ggcttaattg ccggcattgc ggttttaatt
    11221 aagcaggtga tgccgaatat taaagtgatt ggggtcgaat ctaaagattc tgcttgctta
    11281 tatcacgcct taaagcaagg gcaaccgacc gatttagaac gggttgggct ttttgctgac
    11341 ggtattgcgg taaaacgcat tggcgatgaa actttccgcc tgtgtcagca atatgttgat
    11401 gatgtggtgt tggtggataa tgatgaaatc tgtgcctcaa tgaagttgat ttttgaaaat
    11461 gtgcgtgcga tttccgagcc gtccggggca acttcccttg ccggtttgaa aaaatatgtg
    11521 aaagagcatc aattacaaga taaaaactta gcttgcattc tttccggggc gaatgtgaat
    11581 ttccacacct tacgttttgt gtcagagcgt tgtgaaatcg gggagaaacg tgaagcttta
    11641 ttggcagtca ccattgctga acagaaaggt agtttcttaa aattctgcga gattttaggc
    11701 aaccgtgcgg taacggaatt taactatcgc cattccgatg ataaacaagc ctgtattttt
    11761 gtgggggtgc gaatttcagg ctcggcagaa aaagtagaga ttattaaaga tctgcaacaa
    11821 aacggctatg atgtggcaga tttatcggac gatgatattg ccaaaaccca tattcgttat
    11881 atggtaggcg gtagagcaag ctccattcaa tatgagcaac tctatagctt tgaatttcca
    11941 gagcagaaag gggcattgct aaaattctta caaatgctga ctaactggga tatctcgtta
    12001 ttccattacc gagctcacgg agccgattat ggcgatattc ttgccgcatt tgcattaaac
    12061 agaggcgatg aagtagaatt aaaccgacat ttagaacagc taggttaccg cttccaaaat
    12121 gtgagcgaaa gtccggcata tcaatatttt ttaaagtaaa tttttatcaa tcttaggaat
    12181 tagaatggct cgtaaatatt tcggcacaga cggtgtgcga ggtgaagtag gtaaatttcc
    12241 aattacacct gaatttgcat taaaactcgg ttgggcagca ggtaaagtgt tggcaaccca
    12301 aggcacgaaa aaagtattaa tcggtaaaga tacccgtatt tccggttata tgttggaatc
    12361 ggctctggaa gccggtttaa ccgcagccgg tttatcagca gtattcgttg gtccgatgcc
    12421 gactccggca attgcttatt taacccgtac tttccgtgcg gaagccggca ttgtgatttc
    12481 tgcctctcac aatccttatt acgataacgg cattaaattc ttctcgtcgg ttggtgaaaa
    12541 actacctgat gaaattgaag aagcgattga agcaatgctt gataagccta tggattgcgt
    12601 agattcagca aatttaggtc gtgccagtcg tattaatgat gccgcaggac gttatatcga
    12661 gttttgtaaa ggcactttcc cgtctgaact aagccttgaa ggctataaaa ttgtggtgga
    12721 ttgtgccaac ggtgcaacct accatatcgc gccgaatgta atgcgtgagc taggggcaga
    12781 agtgcttgaa atcggtacta agccggacgg tttaaacatc aacgaaaaat gtggtgcaac
    12841 ggatattaaa gcacttcaaa aagtggtggt agaagcaggt gcggatgttg gtctagctta
    12901 cgatggtgat ggcgaccgtt tgattatggt cgatcactta ggcaataaag ttgatggcga
    12961 ccaaattcta tttattttag cccgagaagc tctacgttca ggcaaattac aaggcggtgt
    13021 agtcggtaca ttaatgagta atatgagctt ggaattagct ttaaaagagc tagccattcc
    13081 attctctcgt gctaacgtgg gtgaccgcta tgtactagag cagcttaaag aaaaaggctg
    13141 gaaactgggt ggcgaaaact ccgggcatat tatcgtgtta gataaaaaca ccaccggtga
    13201 tggtattatt gcctcactgc aagtattagc agcaatggca agccacaaat taagcctgaa
    13261 tgatttggca aaagcagtgc ctctgttccc gcaagttctg ctaaatgttc gctttgagaa
    13321 aggggcggca aatccgcttg aaagtaacga agtgaaagcg gtagcggcag atgtggaaaa
    13381 acgcttggca ggcaaagggc gtattttatt gcgtaaatca ggcactgagc cattaattcg
    13441 agtgatggtg gagtgcgaag atgctgtact tgctcaaagc tgtgcggaag agattgttga
    13501 agcagtaaaa cgtaactaat gacaagcggt cgtttttggt aaaaaatttg ccaaatatga
    13561 ccgcttgtta catatatgtg tagaatagat gtagtagggc gaaaattttt ttcgccttgc
    13621 cgaagtgggc aaatgatgat ttgccctaca agtggaggta aaaatggatc aacaagaaat
    13681 gaaaaaaatt gcggcacaag ctgcattaaa atttgtaaaa tcggacacta ttgttggggt
    13741 gggaagtggt tcaaccgtaa actgttttat tgatgccctt gcttcaatga aagatgagat
    13801 taaaggagcg gttgctgcct ctaaagcctc ggaacagcgt ttgcgtgata tcggcattga
    13861 agtattcagt gccaatgaag tgacggaatt agatgtgtat attgacggag ctgatgaaat
    13921 cacgccacaa ggggcgatga ttaaaggtgg cggggcagca cttacccgtg agaaaatcgt
    13981 gagttcatta gcgaaaaaat ttatctgcat tgtggataaa tccaaacaag ttgatgtgct
    14041 gggttcaact ttccctctgc cggtggaggt gatcccaatg gctcgttctt atgtggctcg
    14101 tcagctcgta gccttaggtg gttcgccgga ataccgtgag ggcgtggtca ctgataacgg
    14161 caatgtgatt ttagatgtac ataactttaa aattatggag ccgttgaaaa tggaacatac
    14221 cattaataat attgcaggcg tggtgactaa cggtattttt gcacaacgtt ttgccaatgt
    14281 aaccattgtc ggcacacctg aaggtgcagt tattttagaa taatttatta ttgtaaatac
    14341 aaatacttag gagcaaaaaa tggctaaaat ttctttggat aaatccaaga tcaaattcct
    14401 tctgcttgag ggcgtacatc aaaacgcaat tgatgtactc ttagcggctg gttataccaa
    14461 tattgaagtg cataaaaaag cattagacgg tcaagaatta attgatgcaa ttaaagatgc
    14521 acactttgtt ggtattcgct cgcgtacttt tttaaccgaa gaagtactgg ctcaagctcc
    14581 gaaattaatt gcgattggtt gtttctgtat cggtacaaac caagttgatt tgaatgcggc
    14641 gaaaagacga ggtattccgg tattcaatgc accgttctca aatacccgct cggtggcaga
    14701 attagtgctg gcagagatga ttttattaat gcgtaaagtc ccgactgcaa atgcggaagt
    14761 gcatcgtggg gtgtggaata aatcggcagc aggtgcaaac gaagtacgtg gcaagaaatt
    14821 aggtattgtg ggatatggtc atatcggttc acagcttagt attcttgccg aagccattgg
    14881 tatgcaggtt tatttctatg atgttgaaaa caaactacca ctcggtaatg cacagcaagt
    14941 gccgacatta gagcatttgc tttcaacctg cgatatgatt tcactacacg ttccggaaat
    15001 cgactcgact aaaaacctaa tgagtgcaga acgcattgct caattaaaag aaggctcggt
    15061 gttaattaat gccgctcgtg gtacagtggt cgatttagac gcattagccg ctcggattga
    15121 agacggctca ttgcgtggtg cggcgattga cgtatttccg gaagaaccgg catcaattaa
    15181 tgacccgttc gtgtcaccat tgtgcaagtt tgataacgtg attttaacgc cacatattgg
    15241 tggttcaacc tctgaagctc aggcaaatat cggcaccgaa gttgccaata aatttgtgaa
    15301 atattcagat aacggttcaa ccgtgacggc agtgaatttc cctgaagtat ccttaccgct
    15361 tcatactaat accaaacgtt tattacatat tcacgagaat cgcccaggta tgttaaatca
    15421 aattaaccag atttttgtgg aatcaaatgt gaatattgcc gctcaatatt tacaaaccga
    15481 tgcagctatc ggttatgttg taattgatgt agagagcgac aatgttgatg aagcattaca
    15541 acgtttaaaa gcgatcgaag gcacgattcg agcccgagta ttatattaaa aggcaaagag
    15601 cctgaatcac ttcaggctct tttgtgttac aagcggttat tttttcagaa aactttaccg
    15661 ctaagattag aaacgataag ttgcattcgc ggtgaaagcg ttacctttta attttgtgcc
    15721 gctacgtttt aaattatcgc gagtgtattc tgcacctaat tcaacatcgc ttgaaactgc
    15781 atattttgca ccagcaccga aaccaaagcc acgataggtt tcattaccga ttttgcttga
    15841 gctggcatct acttttacat aaggtaataa atcggatcca acacgataac cttgttgata
    15901 agctatagta tatttatttt tttgttttac atcgctaaat acttttgtac tacctacttt
    15961 tgcttttcct tgtacaacgc ctacaaagtt attgccttgc tcaataccat aatcgacaac
    16021 taatgccgga cctgtggaat ccttgccttt tactccttca actttgtatt ttgttgtagt
    16081 tacatctaca ccgacaccga tgccagtaaa tgtatttccg acaggagcgg ctaatagatg
    16141 ggtagaaaat aagcaagtta atgcgacaat agttaatttt ttcataaaat tactcctatc
    16201 ttgattaagg ttcgatgtta ccacagaaac ggtagccttc accgtgaatg gtgacaataa
    16261 tttctggggt attggggtgg tcttcaaaat gtttacgaat acggcgaata gtaacatcaa
    16321 cggttctatc atgaggtttt agttcccgcc cggtcatttt tttcaataaa tcttcacgtg
    16381 tttgaatttt tccaggatgt tggcaaaaat gtaataaagc cctaaattca ctacgtggta
    16441 atttatttac ctcaccttct ggtgagatta aactatgact gttaatgtct aagatccaac
    16501 cgttaaagcg atagctttca atttcagatt gctcttttac aacgtcgttt tctttatcta
    16561 tcatcgttcg ttgcaataaa tttctcgctc ggatagtcaa ttctcgagga ttaaacggtt
    16621 tagtgatata gtcatcggca cctatttcta aaccgaggat cttatcaatt tcattgtcgc
    16681 gaccggttaa aaacattaat ggtagcttca tattctctcg aatttctcgg gcgagaatta
    16741 atccattttt tcccggtaag ttaatgtcca ttagcacaag attgatttca tttgtactca
    16801 gaacagagtg catttcctcg ccgttagatg cttgaaatac ctgatatcct tctccttcaa
    16861 aaatgctttt cagcatattc cttgttacta actcatcctc aacaattaag atttgtggga
    16921 tcttcatctt ttaaacctct gatttaattt ataaattctg tttaataacg tttatgttac
    16981 aaatttctcg cattttactt ataaatttat gaatgggaag ggtaaaatga caatttgttt
    17041 aattcagtca aatttttggc tatttctctc gatcatcaac acctaaaatt agataatttc
    17101 cattaatatc cgctaggcta cgaaagccga tattataaat agcacattct ctgctgacat
    17161 ttagattgcc ataattaaaa ttatcatggt agtggccatg gaaaattttg ctcgccccta
    17221 attttcttgc tagctggttg attacagaga agccttgagg gtgtggcttt ggtgcttcgt
    17281 gtgtaattaa aatatcggct ttttcatttt ccaatgcttc aaaatcagaa gggaagatcg
    17341 aagagcggtg ccttaatgga ataccaccac gccaaatttt ttccggagca gagtattggc
    17401 aaaaatggat gggatcaaaa aacattggac gattgggcgg catccaaata tgacctcgga
    17461 agacccctcc aatacctgca atttttattc cttgaatgtc aactacttta ccgtgaatat
    17521 tgcgttcttt ccagctacta ttccaaatag aatcaaaggc agcaacggtt ttactgtcat
    17581 ggttaccatg aataaaccaa atatcacaat actgcgaaag ccgatctaat tcatcaggag
    17641 tagtcaattg aagatcgcct agaataacta aagctacctc ctccgtctgc tgttgtagaa
    17701 tcggataaag atgctcgtag ctaccgtgtg gatcgcctgc aaaaagtatc atgttatttc
    17761 ctgtggcagt tattcaaatt cgtgaatatt atattgctta aactgtaatg ggattggttc
    17821 ttcatttaaa atagcttcta ccacctcttt ggcttgattg tagcgatcca aataacttgg
    17881 agattcaatt ttaatataag gaatatggtg tttttctagt agttttttca gtaggtgttg
    17941 aaactgttga cgctgttttt tcgatcctaa actgcgtaaa ccatcggcta cccattttgt
    18001 attgttagaa agtaaaatcg tcacatcaaa aggatattcg cggatcattg aatccagaaa
    18061 cgggtgagcc ttcccttcat actgaataca gaacgcttga gttgtgatgt aatcggtatc
    18121 aataatagcc actttatgag catgtttcat ggcataatca acgtagcgtt gatgtccaag
    18181 taccatttgc ggatagtcgg aatattgcat tgcttgttca ttgccgccaa gttgctcaaa
    18241 cacaaactcg cgcccatatt cccaagcaga ggtagtgtta aatacattcg ctaatttgct
    18301 caccaacact gatttaccac tgctttcacc acctaaaatc gcaatagttt taacaaagaa
    18361 aggacgaact tctttcggaa taaagcgcca gtattgaaaa ggattattac gaatttgtgt
    18421 tgctgaaata ttaaaggatt cacgagtagg atcgacaagt tgaacttcta gatgcaaata
    18481 tttttcataa ggttctttat cttgaatttc actactaaaa acaatagtag gattaatgct
    18541 tttttcttca aatagcgctt taacacgacc agaccaatct tcccagccat ttggatagct
    18601 cggaataccg tcttcaacta aatggtgaat aaagatctgt ttttgttggt atttaaaaat
    18661 ttgctgtacc cagcgtagcc gatcttcatt tgtcggcatt cgtttcattt tactttcctg
    18721 gaaaagacgt aaatcccgtt cagaatcggt acaaacaata acatgtaaaa catctacttt
    18781 actaaatgct tcatagatca tattaatatg acctgtgtga ataggataga atttgccaaa
    18841 aataaccccg gaacgctggt tgctatcttc tacgatatct aaaacggaat gcaaggcttt
    18901 aagcttattg gcgcttggat ttttgatttt tccgcttact aactgattaa aataagctcg
    18961 ggttacccca gcttgttcac aaatatcatt cactttgagt ttaagctgtt tgcgcttttg
    19021 ttgaagataa gcaaaatctg tcataatgat tccctcgatt ttttgatgat tatcatatag
    19081 taaaatgtag catttgaata tatttttatt gtataaatag cagttttata gcatatcttt
    19141 gctttaataa ttagctacac gaccactttc agcactaaaa aaatataaca gattgaattt
    19201 ttttcctttt ttgtagattt ctcgttataa tcacagcaat tttcacttat aaaatggttt
    19261 atcttttatg caacttattc gaggtttcca caatttagac aaacacatcg ctttaaatca
    19321 aggctgtgtt ctctctatcg ggaatttcga tggtgtgcat cttgggcatc aaaatatttt
    19381 ggctcgtctt tgtgatagag ctttagattt aggattgccc tcagttgtga tgttatttga
    19441 gccacaacct cgagagtttt ttgcaaaaaa aagtggaaat tcaactgctt gtaacccgcc
    19501 cgcccgctta atgcgcctgc gagataagct aaaatatttg gaagaagccg gtgtggattt
    19561 tgtgctatgc gtgcgtttta gtgaaaaatt tgctcaaatt tcggcagacg atttcatcag
    19621 cgatttgctc gctaaaagac tgaaagtacg ctatttaagt gtaggcgatg attttcgctt
    19681 tggggcaaaa cgggcgggca gttttgaaac tttacgcaat gccggcttaa aatatcgctt
    19741 tgcggtggaa gaaagccata cccatagctt aaacgaagaa agaattagca gctcattagt
    19801 gcgagaagcc ttacaaaaca atgagttaga tttggcagag aaattactcg gcaagcctta
    19861 ttcgattctg ggacgcgttg cacacggtaa taaactcggc agaaccattg gttttcctac
    19921 cgcaaatatt atgctcaatc gtttggttac gccgattcaa gaggtatttg tggtaaaagt
    19981 gaagaccaaa cagggcagtt ttaacggaat cgccaatgtc ggcaaccgcc cgaccattaa
    20041 cggtactaag ccgttattag aagtgcatat ttttgatttc aatgcctcta tttacggtga
    20101 ggcgattgaa gtgatctttt taaaaaaatt acgcaatgaa ataaaatttc ccgactttga
    20161 agcactaaaa gcccagattg agaaagatcg cttgcaagcg gttgaatttt ttaaggaatt
    20221 tgcaaggtga gcctttctag tttccagccc cagaggggct gagctatgct taacctagta
    20281 cggctatgcc gtgctaggca taaaacagat aattttaacg agaaataaaa aaatgactat
    20341 tgactacaaa aacaccttaa acttgcctga aacaggcttt cctatgcgtg gggatctcgc
    20401 gaaacgtgaa cccgatatgt taaaaaattg gtatgacaaa aatttatacc aaaaaattcg
    20461 tactgcctcg aaaggcaaga aaacctttat tttgcatgat ggtcctccgt atgctaacgg
    20521 taatattcac ttaggtcacg cagtgaataa aattttaaaa gatatgattt tgaaatcgaa
    20581 aaccgcatta ggtttcgata ccccttatgt gccgggctgg gactgtcacg gtttgccgat
    20641 tgagctgaaa gtagaaggga ttgttggtaa accaaatgag aaaatttcgg ctgccgagtt
    20701 tcgccaagcg tgccgtgact atgcgaaaga acaagtagaa ggtcagaaag ccgattttat
    20761 ccgtatgggt attttaggtg attgggataa cccataccta acaatgaatt ttaacactga
    20821 ggcgaatatt attcgcacgc tcggcaaagt gattgcgaac gggcatttat acaaaggttc
    20881 aaaaccggta cattggtgct tagattgtgg atcttcgtta gcggaagctg aagtggaata
    20941 tgaagataaa gtttctccct ctatttatgt gcgttttaat gctgtggacg taaatgcggt
    21001 agaagaaaaa ttcaacgcac agggtaaagg tagaggggca ttatctgcgg ttatttggac
    21061 aactacccct tggactattc cgtctaaccg tgcgattgcg atcaaccctg aattagacta
    21121 tgccttagtc caattaggtg atgagcgtgt gttactagcg gtcgatttag tcgaagatgt
    21181 tgcaaaagca gcaggcgttg aatctgctga gattttagca accaccaaag gcgaaaactt
    21241 agaattatta cgctttaacc acccattcta tgattacagc gtgccattta tttttggcga
    21301 ccacgttacc acagacggtg gtacagggtt agtgcatact gcaccggatc acggtgcgga
    21361 cgacttcgtg gtcgctcgca aatataacat tgaaatggca gggctaattt ccaatgacgg
    21421 taaattcaaa gcagacaccc cgttctttgc cggcttaggt gtgtttgaat ccaacgataa
    21481 agtagtcgaa aaattacaag aagtgggagc attattaaaa ttatcccgca tcaagcatag
    21541 ttatccgcac tgttggagac acaaaacccc gattatcttc cgtgctaccc cacaatggtt
    21601 tatcggaatg gaaacccaag gcttacgcca gcaagcacta ggagaaatca aatcggtgcg
    21661 ttggatccca agctggggtg aagctcgtat tgatacgatg gtggcaaacc gcccagactg
    21721 gtgtatttca cgccaacgta cttggggcgt gccaatggcg atgtttgtgc ataatgaaac
    21781 cgaagaatta catcctcgca ctttagaatt aattgaagaa gtggcgaaac gtgtagaaga
    21841 aaaaggcatt caagcgtggt gggatttaga tcctgcggaa ttgctcggtg aagaagccaa
    21901 agattaccgc aaagtgccgg ataccttaga cgtgtggttt gattcaggat cgacctatgc
    21961 ctctgttgta gaacaacgcc ctgaatttaa cggcaattta gcggatatgt atttagaagg
    22021 ttcagaccaa catcgtggtt ggtttatgtc atctttaatg ctttccactg ctactaacgg
    22081 caaagcacca tacaaacaag tgctaactca cggttttacc gtagatgaga aaggcagaaa
    22141 aatgtccaaa tcgcttggca acgtaattgt gccaagtgaa gtgtggaaca aaaacggtgc
    22201 agatatttta cgcttatggg ttgcctccac cgactatacc ggtgagatta ccgtttcgca
    22261 caatatttta aacagtgccg gggaatcata ccgccgtatt cgtaacacag cccgtttctt
    22321 attggcgaac ttgaacggtt tcgatccaaa aagtgattta gttcaaccgc aagagatgat
    22381 cgccttagac cgctgggcgg tggcttgtgc tttagaggct caaaacgaca tcaaagaagc
    22441 ctatgataac taccaattcc acacggtagt acagcgttta atgcgtttct gctcgattga
    22501 aatggggtct ttctacttag atattatcaa agaccgccaa tacaccacca aagccgacag
    22561 ccttgcccgc cgtagctgcc aaacagcgtt atggcatatt gcagaagcgt tagtacgttg
    22621 gattgcaccg attttatcct tcacagccga tgaaatttgg ggctacttac cgcaggtgga
    22681 aggcagaagt gaatttgtct ttaccgaaga attctacacc ggattattcg gcttaaccga
    22741 gcaagataaa ttagatgatg cttactggca acaaatctta aaagtgcgtg cggaagtcaa
    22801 ccgtgtgctg gaacaagccc gtaaagacaa agtaattggt gctggcttag aagccaaggt
    22861 gacggtattc gccaatgatg aaattcgccc gttattagaa caacttggca atgaattacg
    22921 ctttgtgctg attacctcac aagcgattgt taaaccactg gcggaagcag atattgcgga
    22981 aggcgaatta gccggactcg cagtaaaagt ggaaaatgca gatggtgaga aatgccctcg
    23041 ttgctggcat tatgctaccg acatcggctc gcactctggc cacgaagaag tttgcggacg
    23101 ttgtgtggaa aacgtagccg gcgaaggcga aaaacgcttg tttgcttaaa atgtaattca
    23161 attcatcttt ctaaggcaga ggaaactctg ccttagcttt tccagcgata gatagacact
    23221 cagtttaaaa tttgcaaaaa ttttcggaaa aataaccgct tgtattgatg taaatgatga
    23281 aaataatcgc ttttcttttt atttttctct ccgttttttc tcacactgct ttggctaatt
    23341 ctatggctga acaatgtgtc gcgttaaaat ctaactcgcc ggaaaaaacg gctatttata
    23401 aagctgaatg ggttgagaaa tctgctgagt tgccggctta ctgtttagta caaggtgaaa
    23461 ttggaaaacg ggtgggggtg gataatgttc cttacggtat tcaatttgag ctacgtttac
    23521 ccgaacaatg gaatgaaaaa ttcttatttc agggggcggg cggtgtaggt ggggtgattt
    23581 tccctgccat tggtaagctc tatccgcacg gggcgagtgg tgcaaatggc ttaaacagag
    23641 gctatgcagt agtgagtaac gacagtggtc acgccactcg agatttagtg tttaccgcag
    23701 atcccgaagc tcgctcaaat tatgcctatg cctctatcgg caaagtgacc acggttgcca
    23761 agcagttgat tgccgaattt taccgaaaac cgagcaagca caattacatt atgggctgct
    23821 cgaatggtgg tcgtgaggcg atgatggcgg caatgcgtta tccacaggaa tttgacggtg
    23881 tgattgcagg cagccccggc tttcgggttt cccgttcagt cttggcggaa gtatgggata
    23941 atcgagcctt gttggcagtt gctccgaaga atggtgatgg cgacaaaatt ttatcccaag
    24001 ccctcactca acaagattta gatgtgattg ccaatggtgt attaacacgt tgcgataagt
    24061 tagacggctt agcagacggt ttaattaatg cgtgggaaca gtgtgatttc cagccggaaa
    24121 tggtggcaaa gcaactgggg cagaaaaaag tcgctttaat caaaacgatt ttcgaggggg
    24181 cgaaaaacag tcgaggcgag ctgatttata gcccttgggc gtatgattcc ggcattaacg
    24241 ctaaaggctg gcgggattgg aaattaggtg atagccaaac cgctcagccc aacagcatta
    24301 gttttaaaat gggattgaaa agtttgaccc attactattt agcccccaga gtgccgagcc
    24361 gagatccact cagcgtagat ttggatcaag ccagcgaaca agtgaaagcg gtagggagca
    24421 ttcacgatgc agatcaagtt gatttatccg cttttaagca gaacggtggc aaaatgctga
    24481 tttatcaagg cgtttcagac ccgatttttt cggcagttga tctcaaaaat tggtatcaaa
    24541 gcctacaaca agcggtcgaa aatccgcaac attttgcaaa attgtttttc gtgtcggcaa
    24601 tgaaccattg tgggcgaggg gcaacggtga atgattttga tatgctcact gcccttgaaa
    24661 attgggttga gaaaggactg gagccggaaa gtattgccgc caaagccggt gagctttacc
    24721 ctaataaagc caagcagatc ccgctttgtg cttatccgaa agtcgcaatt tatcaaaccg
    24781 gagaagatcc taatcgtttg accagctatc aatgccgtta agcgaaaatc gctttattgg
    24841 cgatttcagt gtaaaatagc aggcaacgat tgaaaatttt ggaatcaatg atgttaattt
    24901 tgaacaaaca acgtaaagct gaatacctat tgaaaagcct gccatactta ccacaagtgg
    24961 cagagcaggt tcagtttctc gcctcgagca aagcgtttaa acaacaaatt ttacagctga
    25021 ttcaaacggc aaaaaagcgt atttatataa ccgctcttta ctttgaaaaa gatgaagccg
    25081 ggcaggaagt attagctgct ttatatcaag cgaagcgaaa taatcctgaa ctggaaatta
    25141 acattttggt tgattggcac cgagcccaac gtgggcgtat tggtgaggca gagaatttat
    25201 ctaacgccga ctggtatgca aaagttaaat acgaacaagg tttaccggca gggcaagaga
    25261 ttgcctttta cggtgtgtcg attaacggac gagaagtgtt tggtgtattg catttgaaag
    25321 gcttcgtatt tgacgatacg attttgtata gcggagcaag cattaataat gtgtatttgc
    25381 atcaatttga acgttaccgt tatgaccgtt accacgtcat tgaaaataaa gttcttgccg
    25441 atagctgggt gacgtttatt caacagcatc ttttaaccaa tgaagcggta aaacgtttag
    25501 atacggaaaa tcgcccgaaa acggctgaaa ttcgtgcgca aattaagcat ttccgcaaac
    25561 aattaagtcg ggaagcgtat ttatttaacg gtgttgctga aaatggtcgc ttagcggttt
    25621 ctcctttagt aggcttaggg cgacgtaaga atcagttaaa taaagtgatt gaagcgttgt
    25681 tttatcaaac tgagcagaaa ttaacgattt gcactccgta ttttaatttt ccgcattctc
    25741 tgagaacccg tattgaatgg ctattggcaa acggcaaatc ggttgaaatt attgtggggg
    25801 ataaaaccgc taatgatttc tacaccaagc cggacgaaaa attcacgatg gcatcggctc
    25861 tgccttattt gtatgaaaag aacttacgag cctttgcaaa acggttggac ggttatgtac
    25921 aaaataaaca gttaaccatt cgcttatgga aagatggcga aaacagctac cacttaaaag
    25981 gggtttgggt ggataaccgc tatattttgc tgaccggcaa taaccttaac ccaagagcgt
    26041 ggcggttaga tgccgaaaat gcaattttaa tttccgatcc tcaacatcaa ttaagtgaaa
    26101 aagcagaaac ggagttaaac caaatttgcc aacatacgca gattttaact cactacagtg
    26161 atttggaagt tttaaccgat taccctgaaa atgtgcgtaa attgctgaaa aaattcggca
    26221 gggttaagtt ggataaaatt gtgaagatgt tgctttagtt aggtatttat atgggcgagc
    26281 tgatgggttc gcccaattat taaggataaa atatgagcga acaaatttat ggcattcacg
    26341 cagtgaaagc cttttgggat aatgcacctg agcgtttaat tgaagtgctg gtgctaaaag
    26401 ggcgtgaaga taaacgctta atgccactat taaatgaatt acaacgttta ggggtggcaa
    26461 ttcaacaagt gaaccgccaa accttagata acaaatcgca aggtgaagtg catcaaggga
    26521 ttattgccag agtcgtgcca caaaaagagc tgaatgaaca cgatttagag gcgatcttaa
    26581 gccaaaagaa aaatccactc ttgttgattt tagatggcgt gaccgaccct cacaacctag
    26641 gggcttgttt gcgtaccgct gatgcggcag gggttgatgc ggttattgtg ccgaaagata
    26701 aatctgctca gcttacacca accgctcgca aagtcgcctg tggggcagcg gaagtgatgc
    26761 cactgattcg agttaccaat ctggctcgca caatgcgaga tttacaagaa cggcataatg
    26821 tctggattgt cggcacggca ggtgaggcgg aatcgggcat ttatgaagca aaattaaccg
    26881 gctcgattgc gttagttatg ggggcagaag gtgacggaat gcgtcgcctt acccgtgagc
    26941 attgcgacca gcttatcagc attccaatgg cgggctcagt atcttctttg aatgtctcag
    27001 ttgcaaccgg ggtctgtctg tttgaaattg tacggcagaa gctggctggg caataaggaa
    27061 taagtaagaa aaatgcgaag aactgatgtc ttcgcatttt tgtttttaga gccgcaaaaa
    27121 atctcagaaa ttgaaccgct tgttagccca catactcttc caaattaatc actttatcgc
    27181 acgatttctc aattagataa ggatcgtgac tgaccaaaat cagactgcaa tttctcgctc
    27241 ggcactggtt gatcagcaat tctatcgttt cttgttgtgt gattgggtca agccgagaag
    27301 tgacttcatc ggcaaacagt agtaccggtt ctaagagcaa ggctcgcaaa attgctactc
    27361 gttgtagctc gccgccggag acattttccg cattgcgttg caaaatttct gggttgagcg
    27421 aaagttgttg cagtaaactt ggaatcggca gtcgatctaa ttggtgcttg ttaatcacat
    27481 catcaagcaa ggtttttaga ctgacattcg gagcaaaggc ttccggtggg tcttggtaaa
    27541 gcttcaacac ttggtgtttt ttatggctgt ggctgtgcca cacaacttcg cccgatttcg
    27601 gcttaagcaa accacaaagt acatcggcaa gcgagctttt gccaatccca ctatgcccga
    27661 caatcccaag cacttcgcct ttgtgtaagt caaaggaaag gttgctaaac agcgttttct
    27721 taccacgggc aatactcaaa tttttaaccg aaacaagcgg ttgtttttgg cttaaatttt
    27781 gcagatcttg caatggttgc caatgttttg gatcggcact gataagtgct ttggtgtaag
    27841 ggtgttgtgg gttatttaac aacgcttttg cctcaccttg ctccaacagt tcgccttttt
    27901 tcatcactaa aatagtgcca ccgagttgct cggcaacttc aatatcgtga gtaatggtga
    27961 gcaagcctcc gccgttttgg taggcggatt ttaacagttc aatcactttg cctttactgg
    28021 ttgggtcaag ccctttggtc ggctcatcgg caagtaaaat tttgccaccg gcggcagttg
    28081 caatcgcaaa agaggcacgt tgagccattc caccggaaag ctggtgtggg tagttttcaa
    28141 ccgattcgcc cagcccaagt ttggctaaat aggctttgct ttccgttttg gcttgagatg
    28201 atggtttgcc tgccacatag tgaaaacttt cccacaactg cttgccgatt gccataatcg
    28261 gattaagcga gcggcgaggc tcttgtggca gcattaccaa ggttttaccc cacaatgttt
    28321 gcaagcggtc ggattcggtg gaaattttgc aattttttac cgaaatttca ccgcttgcga
    28381 ttaagccgtc gggtaacgcc cccataatcg cttgaatcag caggcttttg cctgagccgg
    28441 tttcgcctaa gatagtaata ttttttcctg cctttaattg taggctaatc ggttcgacca
    28501 gctgtaatct gttttcggtt tcaacagata ggttttggat attgagtaat ttcatcttca
    28561 ttttattact gtaggggcgg gccctgtgcc cgcccataaa tataataaga attttgggca
    28621 ggcacaggac ctgcccctac gcattattta atttttctag acaataattg taagctgagt
    28681 accattaaga aaacggcaac cactggttgc aaaaatatca gcggtgcttc gtagtagtaa
    28741 gggaatagtt cggtcatcat caagcctaat tctgctgtag gcgggcgaag ccctacgctg
    28801 acaaagccaa gtgtggcgag agctaaaatc gcattgccga gcgagaatgc cgataaagtg
    28861 gcaatcatcg gtaataaacg gggcaataag tggcgtttga agctataaaa ccagcccatt
    28921 cccattaggc gtgaggcttg aatttccgag ctgtttgcca ttgttgagct gatagagcgg
    28981 atcacacgga aaaattccac ccacattacc aatgaaatcc ctaaatagag cgtccaaaat
    29041 gagccgggag aaagggcggc aaagagcaat actaaaagta agccggggag tgccatcact
    29101 agatcacaaa taaagctgaa aatgcggtca atccagccac cgaaaaagcc tgccataatg
    29161 ccggtaatta agcctgcaat taaggcacaa atcacgctaa acagtgataa gccaaacgaa
    29221 agacgaatag cagaggcaag acgggcaagc atatcccgcc cgagatgatc ggtgccgaac
    29281 caagcgattt catttggatt ggaaaggata ttggttaaat cttgaaagcc aatatccatc
    29341 gggtaaaaat aaggctgtaa aaaggcaaat cccaacattg caaggagaat agtgccgcct
    29401 aagtattgtg aaattgaaat tttcatcttg atgtcctatc gcttaattcg tgggtcaatc
    29461 caagcactta atagatcggc aatggtgttt aatacaacaa acaatccgcc catcacgagc
    29521 gttgtacctt gaatcatcgg cacatcacgg gcaatgatgg cgtgaaccag tgcgtgtccg
    29581 ctgccgggcc aagcaaatag gctttcgacc accacaacgc cttcaattaa ataaactaat
    29641 tgtactgcgt ggtaggcaat gaccggcacg gcaatattcg gcaaaccgtg acgtaaaaag
    29701 gtatgccaac ggcttaagcc ttttaattgt gaaaattggt aaaattctga gccttttacc
    29761 tgaaccattg cagaacgggt gactcgtacc gaaaccgtac tcaagccaat cgcaagcgtt
    29821 aaggcaggca agataaaatg ttgccaactg ccgtatccgc cggcaggtag ccattttagt
    29881 tgagcggaaa acagcgtaat caagccaatc gcaataataa atgccggcac ggagcgaaac
    29941 agggtactga ataccagtgt caagcggtca aaaaagccgt ttggcttacg ggcggctaac
    30001 aaaccgagcg gaggcccgat aaggattgct aagataagtg ccacaatcgc aaggcttaag
    30061 gtatgcccga attggtgctg aatttcagac cagaccggat caccggtcac caatgatttg
    30121 cctaaattaa attgagccaa gtcacttagc cagctgaagt agctttgcca ccaaggttgg
    30181 tctaatccca gctcggcacg cacagcctca gcagcagcac tatctgcttg atcataacca
    30241 tagcggctgg cagcaatgcg gtatgccata tcacccgaaa gttggcgggt gaggataaat
    30301 gtgagtgtgc caaccgacca aatcaccagt agaatttgga taagacggcg gaagagaatt
    30361 ttgtgcattg tattatttca tttattaaaa agatgatttt atgcgttctg atccccctct
    30421 ttagcaaaga ggggaaggga agatttggca gaagaaataa gcggagaaaa taggtgaaaa
    30481 atgagaaaaa acgataattc tgtcactatt tattcaccta agagcgcaaa ttactactga
    30541 aaaatcctac cttccctttt ctaaagcgag gagtattttg aattaaacta cttacttaat
    30601 aactccaaaa agaagcgtct ttcaaatgga tctagcgtca catttttcac ttctttatgt
    30661 gccaccacat tttgttggta ataaaccact ggaatgattg gcaactcatt gtaaataatt
    30721 ttgctaaccg tttgctttaa tgctttcgct ttttgcgggt cacgctcggt ttcgagcgat
    30781 ttgactgcat tgtctacctc agtattttgc caattcatta ccccccaatc actgccacca
    30841 ttggcgaagt cggaggcaat tacgccatat ggatctaagg ttttgccata atttaaggca
    30901 taaagagcca tttctaaact gccgtcttga tgccccgccg gaatttcgct gaaattaccc
    30961 accgaaacat tcacttccac gccaatttgt ttccattgag cttgtaagat agtagcaatc
    31021 accggtaatt caggacgatc tgagaaggtg cgcatggtaa agctaaacgg tttgccgtct
    31081 ttggttaagc gaccttgttc atcaaaggta tagccggaag cggtcagatt ttgtttaatt
    31141 tttgcaatat cgaccacttg ttctgcggta tcaacacgcc agtcggcaaa ggctttcggt
    31201 aaaatttgat cggcagctcc gttctcaatt tttaagacct gctcagcaat cgccttgcga
    31261 ttaatcgctt gcgataaggc ttggcgaatc gctaagtcgt taaaaaacgg ctttgccacg
    31321 tccattttca actgaatagt acgggcaata gatccgctga ctaattgcag gttaggatcg
    31381 gctttcagcc ttgctacact ggcggtatct aaattaaata ccagtgatga cggctcgctt
    31441 tgtgccatta acatacgggt ttcgctacgg ctgctggcta aataattggc ttgctgaact
    31501 tgcggttttt tgccccaata gccctcaaat tgcaccgctt ccaatttctg cggagcttca
    31561 atttttgtgg gtttaaacgc acctgtaccg ataatttggg tcacttcgcc tttttcatta
    31621 tagctttcag gtgccaaaat taaggcggtg tagtgtgtta aaaaggaagg aaacggcaca
    31681 aaagctttag ttagctcaat ttccactttg ttatctaccg ctcgaatttc cttgataaag
    31741 gcttttgcta acacacccgg tttgcttttt gcgatattca aactatttac cactgcttgt
    31801 gcggtaagta cttgatcgtt atggaatttt accccttcac gcaaggtaaa acgccagatt
    31861 gttccctctt cgttactgcg ccagtcagtg gccaatgccg gaattaaatt cccgttaaga
    31921 tcagcttcga ccaatgtttc cgctaagttc atacgctgga aaattacgcc tgattgattc
    31981 gggtcaaggc tattgatttc ccatggggca accacggttg caagcggtaa tatttccgca
    32041 tttttttgcg attgagtatt ctctacttgc ggtttttctg cggttgcttg agtgctgttc
    32101 ttatcatcgc aagcagaaac acagaacgca gcgacagtta atggaatcaa ccattttttc
    32161 attttatttt ctccataaaa aaatcagggc tatgaatatt ggcgaacttt agcatatttc
    32221 cataaaaaaa gtagagtttt ctgcgagggt atttttgagt ggttaattaa taggcagaat
    32281 aatctcaaga taaggattgt tgatagggga atatttaggt tatttgagga acagaatgcc
    32341 tagtattgta aaattcggaa aaaaattgac cgcttgtgct ttatttgtag gggcgaaaga
    32401 tttttcgccc ctattggttt tagtgctctc tcgttttaaa gaactgtacg tcagggtaac
    32461 gttcttgggc gaggtttaag ttcaccattg tcggagcaat gtaggtgagg ttgtctccgc
    32521 cgtctagggc aaggtttgcc tcgtttttgc gtttgaactc ttcaaatttt ttggcatctt
    32581 tgccttcgac ccaacgggct gtggcaacgt ttacggtttc ataaattgcc tctacaccgt
    32641 attcggtttt cagccgtgaa accaccacat caaactgtag caccccaacc gctcccacga
    32701 tcaaatcgtt gttgattaac ggacggaata cttgcaccgc accctcttcg gaaagttgta
    32761 ccaagccttt gagcagttgt ttctgtttga gcggatcacg caggcggatt cggcggaaca
    32821 gctcaggggc gaagttcgga ataccggtga atttgaaatt ttcgccttgg gtgaaagtat
    32881 caccaatttg gatcgtgccg tgattgtgca agccgataat atcgccggcg taggcttctt
    32941 cggcttgtga acggtcgccc gccataaagg ttaaggcatc ggagatcacc acatctttgc
    33001 cgatacggac gtgtttaagc ttcattcctt tttcatattt acctgaaacg acacgcataa
    33061 aagcaactcg atctcggtgt ttcggatcca tattcgcttg aattttaaat acgaaaccgc
    33121 tgaatttttc ctcgcttggt gaaacttcac gttcgtccga ttgacgggct tgtggtgccg
    33181 gtgcccattc ggttaaaccg tcaaggaaat gattgacccc gaagttaccc agtgcagtgc
    33241 caaagaaaac cggtgtcagc tcgcctttga taaattcggc gtgatcgaat tcgtgcgaag
    33301 cgccttgtac cagctctaat tcatcacgca actgctgggc taaatcatcg ccaacggctt
    33361 gatccagttc cggattattt aagcctttaa ccactcgtac ttcttgaatg gtatgacctt
    33421 gtccggtttg gtataagtag gtttcgtcct tgtaaaggtg atatacccct ttaaacaatt
    33481 ttccgcaacc aatcggccaa gtgatcggcg cacatttgat tttcagcacg ctttccactt
    33541 catctaacag ctccatcggg tcacgaatat cacggtctag cttgttcata aaggttagga
    33601 ttggcgtatc acgcaagcgg gtcacttcca ttaatttaat ggtgcgttcc tcaacccctt
    33661 ttgccgagtc gataaccatt aagcagctat ctaccgcagt caaagtacga taagtatctt
    33721 ccgagaagtc ttcgtgcccc ggtgtatcga gtaagtttac caagcaatca ttgtacggga
    33781 actgcatcac ggaggtggta atcgaaattc cacgttgttt ttccatctcc atccagtcgg
    33841 atttagcgtg ttgtgccgag cctttgcctt ttaccgagcc ggcagtttgg attgcatttc
    33901 cgtagagcaa tactttttcg gtaatggtgg ttttacccgc atcggggtgg gaaataatcg
    33961 caaaggtgcg acgtttatta acttcttgtg ggtatgacat ttttctcaac caattaaaat
    34021 aaaaagggcg gaaagtccgc caaaaaatta agcgttattt tcgctgattt gaggcgagag
    34081 ggcaagcggt taaatttggc ggaaattttg caaaatcggc tagatttgca aaaattttgt
    34141 aaaaatcgac cgcttgcttt ggcacaattt tggaaacagg gtagaataac cagctttgac
    34201 tttattatcg atgtattatc gatgaatcta ttatgatgaa aaaatttatt cctgttatga
    34261 ttgcggctct ggtgttaagc gcctgttcct cccagccggc aaaaaggaaa agcacaccac
    34321 ggattaaagc aaccgctgct tatattccaa ccgaaaattc cgcttatgtg gtgtttgact
    34381 taaaccaaaa gaaatttttg gaaggtaaga atttcaattt agttcgcccg attgcttcgg
    34441 tcacaaaatt aatgacagca gtcgttttct tagaagaaaa ccgctatggg gcgagttgcc
    34501 aaacccaaat tttgccgtca gatgctgatt tcttaaaagg cacgaccagc ccattgccga
    34561 aaaatcttaa cattgcctgt agtgaattgt taaaagcgat gctagttcgc tccgataact
    34621 atgccgctca cgcccttgcc cacgcaacac atttgaataa agcccaattt ttggctcgaa
    34681 tgaacagcaa agcccgtgaa atcggtatga ctcaaaccta ctttggcgat agctcaggct
    34741 tatcttcgga taatgtttca acggtggttg atcttgctaa attggcaggc tatgcggcaa
    34801 gtaagcctga aattcagcaa ttgtctaatt cgccattagt gagtgtttat gcaggtggtc
    34861 gttatttctc tatgaaaaac accaacagta tggttcgttc ccaatcctat caggcattag
    34921 tgagtaagac aggttatacc cgtgaggcgg gttataatct tgcctttatt gctaaagggg
    34981 attgcagagg caagcgaatt ggtgtggtta gtttaaacaa tgataacgct gcttcacgca
    35041 ccgatttcac tgaatcgatg ttagcgaagt atcaatgcgt accaaatccg tatagacaat
    35101 atgattaatg gttgccttga gaaaatagcg gtttttctct aaaatactgc cttttaaatt
    35161 aaatgaattg agacaagatt gtgtcagaaa ttaaattaat tgtcgggctg gcgaacccgg
    35221 gggtaaaata tgaagaaacc cgccataatg caggcgagtg gctgattaac gaacttgccc
    35281 gaatatataa cgttagccta aaagaagagc cgaaatattt tggcaaagta gcaaaaatta
    35341 ataccgctca aggcgaagtc cgtttattag tgccgactac ttttatgaat ttaagtggta
    35401 aagcagtggg agcgttagcc aatttttatc ggattaaacc ggaagaaatt ttggtcgctc
    35461 acgatgaatt ggatttacca cccggttcgg tgaaattgaa aaaaggcggc ggacacggtg
    35521 gtcataacgg cttgaaagat attattgccg cccttggcaa taataaagag ttttaccgtg
    35581 tgcgaattgg tattggacac ccggggcata aagacttagt ggcgaattat gtgctgggca
    35641 agccttcacc aagcgatcag acattactga atagtgcggt tgatgaagcc gcacgttgcg
    35701 tagaagtttt atttaaagac ggaatggtta aggcaactaa ccgtttaaat agttttaaag
    35761 caaattaaaa ctctctccct tgtgggagag agaccgaaaa atacttcgtt cagaagtatt
    35821 tttcagagag aggggagcgg tcatatttga ggaaattatt gcaatttcgc cctctccctc
    35881 attttcgctt ctgaacgaag ctcaaattta tccctctccc gcaaggggag aggggaattc
    35941 taaggaacaa aaaatgggat ttaaatgtgg tattgtgggt ttaccaaatg tcggtaaatc
    36001 gacccttttc aatgcattaa ctaaagcagg tattgaggca gcaaactatc cgttctgtac
    36061 gattgagcca aataccggtg tggtaccaat gcctgaccct cgcttagatg cgttggcgga
    36121 aattgtgaaa cctgagcgag tgttaccaac tacaatggaa tttgtggata ttgccggctt
    36181 agtggcaggt gcaagcaaag gcgaaggttt aggtaataaa ttcttagcca atattcgtga
    36241 aactgatgcg attggccacg ttgtgcgttg ttttgaaaat gatgacatcg ttcacgttgc
    36301 ggggaaaatt gacccagctg atgatattga aatcatcaat accgaattgg cacttgccga
    36361 tttagacagt tgcgagcgtg ccattcagcg tttacaaaaa cgtgcgaaag gtggcgataa
    36421 agacgcaaaa tttgaattat cgattatgga aaaaatccta cctgtgcttg aaaatgccgg
    36481 tatgattcgt tcggtagatt tagataagg
//
LOCUS       DANJWC010000006        32539 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.006, whole genome shotgun sequence.
ACCESSION   DANJWC010000006 DANJWC010000000
VERSION     DANJWC010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 32539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32539
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.006"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            77..373
                     /gene="fis"
                     /locus_tag="PWX02_000181"
     CDS             77..373
                     /gene="fis"
                     /locus_tag="PWX02_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HDL1728242.1"
                     /translation="MLEQQPTQNPLTVTMLNAQAQQVNKPLRDNVKAALKNYLSQLNG
                     EDPTELYELVLSEIEHPMLDMVMQYTRGNQTRAATMLGINRGTLRKKLKKYGMG"
     gene            complement(478..3276)
                     /locus_tag="PWX02_000182"
     CDS             complement(478..3276)
                     /locus_tag="PWX02_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin family TonB-dependent
                     receptor"
                     /protein_id="HDL1728243.1"
                     /translation="MKEHTFHYSPIAIAVIFSVSHSQLFANEHQMTNDHSTVLDEVIV
                     TESHYAHERQNEVTGLGKVVKNYHEMSKNQILGIRDLTRYDPGISVVEQGRGASSGYA
                     IRGVDKNRVSLLVDGLPQAQHYHTLGSDANGGAINEIEYENIRSIELSKGASSAEYGS
                     GALGGAIGFRTKDAQDIIKEGQHWGLDSKTSYASKNSHFLQSIAAAGEAGGFEALVIA
                     THRHGKETKIHSEANKLKHNIRRITGFENRYDLYPDSAQNAPGGSFFIVEDTCPTLDC
                     TPRARPKLNRDNFPVRTFPEYTPEERKQLEQIPYRTEQLSAQEYTGKDRIAPNPLDYK
                     SNSVFMKFGYHFNSSHYLGAILEDTKQRYDIRDMQTPAYYTKDDINIRVDNLSLKNSS
                     VYEGDNILDGLVFNKRIPYGLRYSHVKFFDEHHHKRRLGFTYKYKPENNRWLDSIKLS
                     ADKQDIELYSRLHRLHCSDYPVVDKNCRPTLDKSWSMYRTERNNYQEKHRVIHLEFDK
                     ALNAGQGVFKQTHKLNLGLGFDRFNSLMDHGDITAQYTKGGYTSYRGRGRLDDPYIYR
                     RNPRSIETVSLCNNTHGDIINCEPRKIKGGSHFVSFRDLITSEYVDLGLGVRFDQHRF
                     KSDDPWTLSRTYRNWSWNGGITLKPTEFMSLSYRISNGFRVPAFYELYGKRDHIGLKD
                     NEYVQREQRSHQLEPEKSTNHEIGVSFKGQFGYLDVSYFRNNYKNMIATACKRIIQKS
                     HCFSNYHNIQDVALNGINLVAKFDLHGILSMLPDGFYSSVAYNRVKVKERKLNDSRLD
                     SVNDPILDAIQPARYVLGFGYDHPEEKWGIGITTTYSKAKNADEVVGTRHHGIYRVDL
                     GGKLTGSWYTHDITGYINYKNYTLRGGIYNVTNRKYSTWESVRQSGVNAVNQDRGSNY
                     SRFAAPGRNFSLAFEMKF"
     gene            complement(3364..5130)
                     /locus_tag="PWX02_000183"
     CDS             complement(3364..5130)
                     /locus_tag="PWX02_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HDL1728244.1"
                     /translation="MFKIKNSVILLNAVLLSACTSNGGSFDTNSVKASSSYSSSSKDS
                     TAQPKSERRTISSTESLAQPSLGFQTKIPRRNFFGGTGEENGAIGEITPIVGEPGKPL
                     SLEEEMKKLEEGVKKFGYDEDDIIHTHDGKKLHRKREMKFVRSGYFIHLGAAAETKRE
                     EPRLLKTGPTGYVYYLGTQPSKAFPTQVATYEGTWDFTTNASTKNKSKYFEGGDAGTN
                     VGATPENNQANTEEEDNPIGHSSKFEVNFSDKTLKGTLTKNGYVNPRKKDKQEITDHY
                     KIEANIHGNRFNGKAEALKTDDPYFGKDSNTVEGGFYGDKAQELAGKFLADDKSIFVV
                     FAGKRDEKGEDKVEKKFDSVQVSLKDLAKSDMDTFGLATHLVIDGVQIALLPEGKTRF
                     ADMDFYQVLTKNINGKDYKITVCCNNLDYVKFGTFEQKGKDGHQYLVGERTAVADLPK
                     EARIYRYTGTWNGSIRSKDERVGGDSPSDKAHGTRSIFNVNFADKKIEGKLIANDGLE
                     TNPMLTLDGKIEGNGFSGTAKTGEKGFNIDNKSTAGGTDVHLNAKFEGGFYGPQAAEL
                     GGIVHSAETNKDKVSITFGGKR"
     gene            complement(5265..5930)
                     /gene="rnt"
                     /locus_tag="PWX02_000184"
     CDS             complement(5265..5930)
                     /gene="rnt"
                     /locus_tag="PWX02_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HDL1728245.1"
                     /translation="MENITESIPQSTTPPTDYNLLKNRFRGYLPVVIDVETAGLNAQT
                     DALLELAAITVKMDENGYLVEDQKCHFHIKPFEGANINPESLKFNGIDIDNPLRGAVA
                     ENIAIPEMFKMVRRAMKEQGCQRAIIVAHNATFDQSFLQAAIKRINAKRDPFHPFAMF
                     DTATLAGFMYGQTVLVKACQAAQINFDGHQAHSALYDTEKTTELFCAMVNKLKDLGGF
                     PHI"
     gene            complement(6012..6419)
                     /gene="gloA"
                     /locus_tag="PWX02_000185"
     CDS             complement(6012..6419)
                     /gene="gloA"
                     /locus_tag="PWX02_000185"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDL1728246.1"
                     /translation="MRILHTMLRVGDLERSIKFYTDVLGMRLLRRSENEQYKYSLAFL
                     GYADESESAVIELTYNWGVDSYELGTAYGHIALGVDDIYQTIKDVRAAGGNITREPGP
                     VLGGTTVIAFAEDPDGYKIEFIENKNAQAALGN"
     gene            6509..7324
                     /locus_tag="PWX02_000186"
     CDS             6509..7324
                     /locus_tag="PWX02_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728247.1"
                     /translation="MAHDHDSPSKLEISTHVALPMVLMLFIEMALNFYSAPKNSIFIS
                     PYLLAFFVTSLISFLVLWKGEICRGQASRLSFILPFLLIFAVGNFLYSIGFTPKHNPM
                     AVASIAAVFACLIYFRLPEDQTLYNTLMYCGLAISSIGIIQYLAIYWFELPSLFNGIR
                     ANNFAQVLLGILLAGWYLMLAKSRLDIFLKLLVKTALVVLILNYCWTAFVLYQHLQIM
                     PDMPLLSFSIYFAAQFVILLMLAWLLLGKNIKNPTAWTVATLLALLYPFANVV"
     gene            7328..7597
                     /locus_tag="PWX02_000187"
     CDS             7328..7597
                     /locus_tag="PWX02_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728248.1"
                     /translation="MKRALKFFGIATVLSVAAIYYFLMPSHYLATPINWILPKAWKVQ
                     LPQGLNLNWQGSSLAQFQLSYRDCPLLSADKTDLGWIEKNHLFCR"
     gene            8262..10037
                     /locus_tag="PWX02_000188"
     CDS             8262..10037
                     /locus_tag="PWX02_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH domain-containing protein"
                     /protein_id="HDL1728249.1"
                     /translation="MPLKTAIRISLLSQNSWGKGNIVISNSDGEITEKGLNLPLRLTG
                     NIKHGNFILYSSVPLDFQGNFDDLSVKFLPSSLLRLTGKERYLTIEDLRFPLAGIKID
                     KHGIHGRLHAILRGQSPDFNNIEFHLDGQAQNFKAGALDFFQDPKDANAVKDSWKWRI
                     WGASILKAFNSKVNLSGRGQWHKQLVRLEELKGDLATVKLADTTISKITLNNTQAIRF
                     NVKKFTLDGAVMLQSPEIAFSYGGVLPKPRVNLAFDGEVEKLRFKGAVNTTELGPLKL
                     FARRQLSQDASQIIGKLYWPEQSAQGFQPLFPFRSQWVINNGTIRGETAFSAGSKNGL
                     IAGGHLAIRNGGLSLPNGEAKGIEFSLPYRYQNGRFHFGAKKPLDVKIAQIKLGDLAL
                     DNASMKLNGYYPYTKAKPLNLNQLSFDLFGGKLNVKRFALPQTELAYLNLERIDFEQI
                     LQFMQYQQIDLKGKANAILPFWLSGKPCYICDGLLTQAETSYLTFTPELLSEMQKSGY
                     TEQILLHLVDKSTINDFRSLINVGPKGDLVLDGKLKLSSNQNKSKVNLNYNHRENLFD
                     LWHLINYGSQFEQKLENYLYQKLER"
     gene            10055..10249
                     /locus_tag="PWX02_000189"
     CDS             10055..10249
                     /locus_tag="PWX02_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HDL1728250.1"
                     /translation="MKPATFYFAIFLLNLTACTPKVQLETPSEGITINMNVVVDHRID
                     VKMDEKSRAVITKSENKTKN"
     gene            complement(10246..10719)
                     /gene="folK"
                     /locus_tag="PWX02_000190"
     CDS             complement(10246..10719)
                     /gene="folK"
                     /locus_tag="PWX02_000190"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDL1728251.1"
                     /translation="MQTVYIALGSNLENPLEQLKQAVEKLKKLANEFEISPFYGSKPV
                     GPQDQPDYVNAVAKFSTDLSPTALLDKLQSIENEQGRVRVRRWGERTLDLDIILYGNQ
                     QIQTERLNVPHIEMKNREFVIVPMFDLTPDLVLPTGEKLADIYAQFKDHQMIIFS"
     gene            complement(10922..12253)
                     /gene="pcnB"
                     /locus_tag="PWX02_000191"
     CDS             complement(10922..12253)
                     /gene="pcnB"
                     /locus_tag="PWX02_000191"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HDL1728252.1"
                     /translation="MLHKKFAVNASQYRISPKDFPKNAISIVDKLQIAGYEAYIVGGC
                     IRDLLLEKKPKDFDIATNARPEEIQRIFGRQCRLIGRRFRLAHIVYGREIFEVATFRA
                     GHESHRNDKISKVSDEGMLLRDNVYGTLRDDAERRDFTVNALYYDIKRNLIFDFFNGI
                     EDLRAGKLSLIGEPAIRYQEDPVRMLRAVRFMAKLDMFLDKATASPIRDMAALLTNIP
                     AARLFDESLKLLQTGAGLKTYQLLREYELWQFLFPVLPAFFTVKGDSYAERMITKALN
                     STDERIADKLPVNPAFLFAALLWYPLREKMEQLKNEGGFNSHDAMMLAANDVLAESCK
                     AIALHRRHTSVIRDIWALQFQLMKRTGNRLQQTLEHIKFRAGFDLLVMRAEIEGGDLV
                     ELSAWWHEYQFSNDGQRTELLKSVRQSGSVPSEKKKTRRRRTFKKKSKVNG"
     gene            complement(12498..12944)
                     /gene="dksA"
                     /locus_tag="PWX02_000192"
     CDS             complement(12498..12944)
                     /gene="dksA"
                     /locus_tag="PWX02_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HDL1728253.1"
                     /translation="MTQAAKTSLGLLALAGVEPYELKKDEEYMNEAQREHFTKILKAW
                     HSQIMEEAERTKSQLQEDMTNFADPNDRASQEEEFSLELRNRDRERKLLKKIEQTLYK
                     IQEDDYGFCETCGVEIGIKRLEARPTADLCIDCKTLAEIREKQLGL"
     gene            complement(13194..14147)
                     /gene="thiB"
                     /locus_tag="PWX02_000193"
     CDS             complement(13194..14147)
                     /gene="thiB"
                     /locus_tag="PWX02_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HDL1728254.1"
                     /translation="MKFILSSLALLSASVYANQPTLTVYTYSSFASEYGPAPKLEQNF
                     EKECGCDVKFVPLDDSVTMFNRLRLEGAKTKADVVVGIDNFLQPEAEKSGLFAEFEAK
                     TKLETQQKIFFPYDTGSYAFIYDKEKLKNPPKSLKELVERQDLKVIYQDPRTSSVGRG
                     FLMWVNQVYGDEAPTAWKTLAKHTVTVGKGWSETYGAFLKGESDLVLSYTTSPLYHKW
                     HENTDKYVAAPFEEGHLVQVENAAIVKSSQQKALANQFLEFLHKPESQHIISYHNVMN
                     PVLTDGVDPAFAAMPTYKTSEFTPPTTETIQKWLADWSKSW"
     regulatory      complement(14191..14334)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            14618..15604
                     /locus_tag="PWX02_000194"
     CDS             14618..15604
                     /locus_tag="PWX02_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDL1728255.1"
                     /translation="MSQLIRTLKGHIRDEIIKKGGWVNAHAHADRAFTMTPEKITVYQ
                     NANLQQKWDLVDEVKRNSSVEDYYRRFSQAIELMISQGVTAFGTFVDIDQVCEDRAII
                     AAHKAREVYKNDIILKFANQTLKGVIEPNAKKWFDIGSEMVDIIGGLPYRDEVDYGKG
                     LEAMDILMDKAKSLGKMLHVHVDQFNSPKEKETEQLCDKSIEHGMQGRVVAIHGISIG
                     SHPKEYRKMLYQKMRDSQMMVIACPMAWIDSGRKEDLQPFHNALTPADELIPEGITVA
                     IGTDNICDYMVPLCEGDMWQELSLLSAGCRFTNLEEMANIASINGRKVLGLV"
     gene            complement(15659..16078)
                     /locus_tag="PWX02_000195"
     CDS             complement(15659..16078)
                     /locus_tag="PWX02_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HDL1728256.1"
                     /translation="MLGNILGSLASSVLSGNNQSTATQLIQNLLASQGGIEGLIAKFQ
                     QGGLDDVLRTWISSDEDNAPVSGEQISNVFGQENIQNVAQEAGVDAADASDLLAQFLP
                     KIVDTLTPNGNVEDLKNFDANDLLAQAAKGILGNLFK"
     gene            complement(16145..16900)
                     /gene="kdsB"
                     /locus_tag="PWX02_000196"
     CDS             complement(16145..16900)
                     /gene="kdsB"
                     /locus_tag="PWX02_000196"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HDL1728257.1"
                     /translation="MKFTVIIPARYASSRLPRKPLLDIAGKPMIQHVWEKAQLAGASR
                     VIIATDHPEIEALAQSFGAEVCMTSEKHNSGTERLAEVIEKMQIADSEIIVNIQGDEP
                     LIPPVIVTQVAQNLDAYQVNMATLGVKLATREELFNPNAVKVVADKNGMALYFSRASI
                     PFARDSFPDCDDSFVAAQGYLRHIGIYAYRAGFVKQYVAWQPTQLEQLESLEQLRALW
                     HGEKIHIEPAKEAPQVGVDTPEDLERVRAILAR"
     gene            complement(16910..17863)
                     /gene="trxB"
                     /locus_tag="PWX02_000197"
     CDS             complement(16910..17863)
                     /gene="trxB"
                     /locus_tag="PWX02_000197"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDL1728258.1"
                     /translation="MTTKHTKLLILGSGPAGYTAAVYAARANLNPVLVTGMQQGGQLT
                     TTDEIENWPGEFEQITGTELMNKMLKHAEKFETEIVFDHIHSVDLSKRPFTLNGDLYT
                     FTCDALIIATGASAKYLGLPSEEHFKGKGVSACATCDGFFYRNKPVAVVGGGNTAVEE
                     ALYLANLASEVHLIHRRDSFRAEKILLGRLQKRIEEGKIILHTDRTVDEVLGDNMGVT
                     GLRLKSTKEELSEEIKVDGFFVAIGHAPNTAMFEGQLELENGYIKVKSGLEGNATATS
                     VEGVFAAGDVMDHNYRQAITSAGTGCMAALDAERFLDAQEA"
     gene            18030..19100
                     /gene="dhaK"
                     /locus_tag="PWX02_000198"
     CDS             18030..19100
                     /gene="dhaK"
                     /locus_tag="PWX02_000198"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDL1728259.1"
                     /translation="MKKLINSIETVLDEQLSGFVKAHSDLTLNTEPVYIYRTTPSNQP
                     KVALISGGGSGHEPMHAGFIGKGMLDGACPGAIFTSPTPDQMFECGLAVDRGEGVLLI
                     IKNYTGDVLNFETATELLADSGVKVATVLVDDDVAVKDSLYTAGRRGVANTVLIEKLL
                     GAAADKGYNLSELAKLGHQLNNNGHSIGIALSACTVPAAGKPSFTLAENEMEFGVGIH
                     GEPGIERRPFENLDSTVKQMFETLIAHGDYQRKIRHWNTQTQQWDEIEESKVALKKGD
                     RVIALVNNLGAVPLSELYAVYDALEKECQTFGLSIERSLVGSYCTSLDMQGISITLLK
                     VDEDILKLWDAPVNTPAMRWGE"
     gene            19118..19744
                     /gene="dhaL"
                     /locus_tag="PWX02_000199"
     CDS             19118..19744
                     /gene="dhaL"
                     /locus_tag="PWX02_000199"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HDL1728260.1"
                     /translation="MTISKQQILKWLENCQQVMTEKRDYLTELDTVIGDGDHGLNMQR
                     GFSKALEKAHTVQDKDIGTLLKTVGMTLLSQVGGASGPLYGSFFIKAAQGATGKEQLS
                     FNELLALLQQGAEAIVQRGHAQVGDKTMCDLWLPLLEDLAQADSTQPIAQILLQAVEK
                     AENFANQTAPLMAKKGRASYLGERSIGHKDPGATSSYYIFKALAEALQ"
     gene            19754..20161
                     /gene="dhaM"
                     /locus_tag="PWX02_000200"
     CDS             19754..20161
                     /gene="dhaM"
                     /locus_tag="PWX02_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDL1728261.1"
                     /translation="MVNLVIVSHSAKLAEGVGEILAQMAQGGCKIALAGGIEDTSNPI
                     GTDAVKIMQAIESVFSPDGVIVFVDLGSAILSTQTAIDLLEPELAEKVIISYAPLVEG
                     AFAAVVAASAGDSLEAVLNEANEAAALKQEQAN"
     gene            complement(20201..21046)
                     /locus_tag="PWX02_000201"
     CDS             complement(20201..21046)
                     /locus_tag="PWX02_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone YbbN"
                     /protein_id="HDL1728262.1"
                     /translation="MDYFVNVNEQNFSEVWAAAAQVPTVFHFISPREVASLEMDALLR
                     RISDENPNQFLLATVNCDEQQALAAQFRIQQIPTLYLFAGGQPIDAIQGAVSEQELRI
                     RLANILPKEEELKFQQALELLQDQRYTEALPLLKSAWEMTDKKNSDFALLYAETYIAM
                     KSVEEAREILKAIPIQDRDSRWHGLQAQIELLEQASESPELQQLQADYANSKTPEIAI
                     KLANQLHQAHKNEEALTLLFDWLKSDLNVANGEVKSQFLAILSALGTDPIVPKFRRQL
                     YSLLY"
     gene            complement(21090..21416)
                     /gene="glpE"
                     /locus_tag="PWX02_000202"
     CDS             complement(21090..21416)
                     /gene="glpE"
                     /locus_tag="PWX02_000202"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HDL1728263.1"
                     /translation="METTFKEISPQQAWELIENEGAVLADVRDARRYVYSHPQDAFHL
                     TNQSYGKFLDEVDYDEPVVVICYHGVSSQSTAQFLIEQGFEKVYSVKGGFEAWERNGL
                     PIETSY"
     gene            21567..22025
                     /gene="exbB"
                     /locus_tag="PWX02_000203"
     CDS             21567..22025
                     /gene="exbB"
                     /locus_tag="PWX02_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HDL1728264.1"
                     /translation="MEQLFQFLEHHTDFVILGLLGLLSVICLWKAIERIMFYSKVDIS
                     KYDTLEELDIDLTHNLTTISTIGSNAPYIGLLGTVVGILLTFYHLGQSGGDIDAASIM
                     VNLSLALKATAVGILVAIIAMVFYNGFGRKVEENKLKWLALQSKKPHQAV"
     gene            22062..22499
                     /gene="exbD"
                     /locus_tag="PWX02_000204"
     CDS             22062..22499
                     /gene="exbD"
                     /locus_tag="PWX02_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HDL1728265.1"
                     /translation="MKKFDEINIIPFIDIMLVLLAIVLVTASFISQGKIQVNVPKAST
                     TQPMKADDLAKLLTITENNEFFFNDQAITKEQLIAEVATWDKSQKVSLKVDGAVAFEK
                     FVELTDILSANEIKNVAIITKKETAPAPSSTPGSPAQVPAVAP"
     gene            22515..23414
                     /locus_tag="PWX02_000205"
     CDS             22515..23414
                     /locus_tag="PWX02_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HDL1728266.1"
                     /translation="MKKKHSRIGLLASLAIHTALIGGTFAWLHNHKVEKIAEQNSLSM
                     EMVAALLEQPQVAVAPDPITEEVKEIEPEKAEVPPEPKLTPEPTPEPEAIPDPTLNPK
                     KEKPKEEKRKERHHHKHKHRHKEKREEEKPKDKPKERPKHHHKHIKALERGPEIKQGI
                     VAKAIPNAAEGEKVVAGVVGGQKNGSPNSTSTTGSANTGASSGNASELAAYKVALQRA
                     LQRKANNSYPQREKMMRKTGTVTIKFTVTSSGSITNVSVLNSSGNSNLDNAAVRSAEG
                     LSMPPPPAGFPPTVTVPVRFALE"
     gene            complement(23483..24364)
                     /gene="cysZ"
                     /locus_tag="PWX02_000206"
     CDS             complement(23483..24364)
                     /gene="cysZ"
                     /locus_tag="PWX02_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HDL1728267.1"
                     /translation="MSSVFSEPQNQVGLGFHYFIYGWRLLLQRQFLPFVIFPILINTV
                     LMVGLIWGFFANIGGLLDMLLPSWLEWLSFVLIPLIFLMILVGFYFAFTTLANFVAAP
                     FNALLAEKVELQLTGEPLGDMSFGAMLKDVPRMLKREWQKMMYSIPRLIALFVLGFLP
                     VVGQTVAPVLTFIFGAWLIAIQYCDYPFDNHKISFQRMRNALSQSRTLNFTFGALVSL
                     FTMIPFVNLVVMPVAVCGATALWVKEYRNFFLNNQTGEFSKADYTFTKVNSTSLSTET
                     RTGEVSPNVRNGDIRKQ"
     gene            24476..25378
                     /gene="zipA"
                     /locus_tag="PWX02_000207"
     CDS             24476..25378
                     /gene="zipA"
                     /locus_tag="PWX02_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HDL1728268.1"
                     /translation="MELHIIFFILAGLLIAVLIGFSLWSARREKSNIFSNTFSTRPIA
                     VPPQNNKMSNSIPTSLQPQNEDYQAMSVPEENPQQIQQEVESSLQNIKISLPGGNPQQ
                     QPEQIQPIQSVAEPTVSTYDQKISNQQHIQYNTPDFANPMVNTVVEETPQAVAIEPQE
                     TQSAEEENPFVTLYLYAPQNEAFRGDFVVHYLEEVGLRFGEHQIFHRHQHIDDSLSPI
                     IFSAANLMNPGTFDLNNISNFATAGLVLFMQLPTEGNDVANMKLLISNAEHLAASLGG
                     FLYDDNQQPFGEESRQRYMQRVMH"
     gene            25443..27500
                     /gene="ligA"
                     /locus_tag="PWX02_000208"
     CDS             25443..27500
                     /gene="ligA"
                     /locus_tag="PWX02_000208"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDL1728269.1"
                     /translation="MSLFNQTSLFEENATPELVEKIETIRQTLRQYEYEYHVLDNPSV
                     PDAEYDRLMNELKNLEWQHPELISADSPTQRVGAKPLAGFAQVTHEIPMLSLDNAFSD
                     EDLDGFLRRMESYITASPSQLEFCCEPKLDGLAVSILYENGILTRAATRGDGTTGEEI
                     TANIRTIRNIPLKLNTTNPPARLEVRGEVFMPQSGFNELNEKALAKGEKTFANPRNAA
                     AGSLRQLDPKITRQRPLVLNAYGIGVYESDDELPATHFERLQWLKSLGIPVNNEIRLV
                     QGKAALLAYYAEIQAKRPTLGYDIDGTVLKVNDIALQEQLGFISRSSRWAIAYKFPAQ
                     EEMTILKEVDFQVGRTGAITPVAKLEPVFVAGVTVSNATLHNGDEIERLGIVIGDTVI
                     IRRAGDVIPQITGVVLERRPENAKKIEFPTACPICHSAVTRVEGEAVARCTGGLFCEA
                     QRKEALKHFVSRKAMDIDGVGEKLIEQLMARELIETPADLFKLDHTTLMRLERMGEKS
                     AQNALNSIEKAKNTTLPRFLFSLGIREVGEATALNLANHFGSLEAIRQATFEQLKEVQ
                     DVGEVVANRIVSFWQEPHNVEVVEDLIAQGVRWENVVKAEIVDNPLKDKNVVLTGTLT
                     QLTRDEAKALLQSLGCKVSGSVSSKTDYLIAGEKAGSKLAKAQELGIQILSEQQFIEL
                     TQH"
     gene            complement(27563..28831)
                     /locus_tag="PWX02_000209"
     CDS             complement(27563..28831)
                     /locus_tag="PWX02_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDL1728270.1"
                     /translation="MTQQIEHSTWQSKFAAMGPGILMASAAVGGSHIIASTQSGAIYG
                     WQLAIIILLANLFKYPFFRFGVQYTLASGKTLLEGYKEKGKFYLIIFFILNVFAAMIN
                     TAAVAIVSAAILKFIFSALGIPLDLSIPMAGTIVIVVTWGILLLGQYKFLDTLSKWIM
                     IALTISTVSAVIIAALKGGVEIAPDFVEPSPWNLAALVFIVALMGWMPAPIEISAINS
                     MWVIAKKRLSKVNYEDGIFDFNVGYIGTAILAIVFLALGALVQYGSGEKVEQAGVAYI
                     AQLIKMYAFAIGDWSKLLIAFIAFMCMFGTTITVIDGYSRANAEALRLISGRKQHSQT
                     ELSIWMTVTALIGILIISQFMSDVAKMLQFAMISSFVSTPVFAWLNFSLVLKGEHRAK
                     GWLFWLAVIGLIYLTSFTLLFLAQQFKLIG"
     gene            complement(29155..29865)
                     /locus_tag="PWX02_000210"
                     /pseudo
     CDS             complement(29155..29865)
                     /locus_tag="PWX02_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247995.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
     gene            complement(29834..29980)
                     /locus_tag="PWX02_000211"
     CDS             complement(29834..29980)
                     /locus_tag="PWX02_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728271.1"
                     /translation="MIKPLDSVYWRGKDVSEFFARKRLPDTAVQCVKDNELSYVYGYS
                     TSFV"
     gene            complement(30098..31315)
                     /gene="fabB"
                     /locus_tag="PWX02_000212"
     CDS             complement(30098..31315)
                     /gene="fabB"
                     /locus_tag="PWX02_000212"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HDL1728272.1"
                     /translation="MKRVVITGLGVISSIGNNKEEVLESLKAGKSGIEFVPEFAEVGM
                     RSQVAGTIKLNPAELIDRKIYRFMGDAAAYAYLSMKEAIEDSGLTEEQVSNDRTGLVI
                     GAGTGSAHSQLVACDAVRGPRGVKAVGPYAVTKTMASSVSACLATPYKIRGVNYSISS
                     ACATSAHCIGHAMELIQLGKQDMVFAGGAEELSWECATEFDAMGAVSTKYNETPTKAS
                     RAYDANRDGFVIAGGGAVVVVEELEHALARGAKIYAEIVGYGATSDGYDMVAPSGEGA
                     ERCMRQAMATVNGEIEYINVHGTSTPVGDVKELGAIRNVFGDKTPAISSTKSMTGHSL
                     GAAGAHEAIYSLLMLDNNFIAPSINIETLDEQAQGMNIVTERQDKALTTVMSNSFGFG
                     GTNACLVFQRYSK"
     gene            31576..>32539
                     /locus_tag="PWX02_000213"
     CDS             31576..>32539
                     /locus_tag="PWX02_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDL1728273.1"
                     /translation="MKTLTLTANKITAVEGAAIGANQGSFAQKLNSTKARIDQLNAYR
                     IERAINANNHHFMHVFSLIPLLIHLNHPVLPAYVENAPQGIWHFQLSDYQKQFLVSYR
                     FDEAISADHLGPHPSFDALYSMGSTGTITQTSCSDLDLWLCYPNHFTIEQYKLMELKI
                     NKLQEWAKTFQININIYLMNPEQFKSQTYNSSVTDEHSGSAQHFFLLDEFYRSAILLA
                     GKRLLWLHLHKNEYQNVHNNPEIDLTEWIDFGDFSSLSTSEFFGASLWQLYKGINNPY
                     KSAIKILLLECYAHTYPKTKLISKEFKKKLLSDNALEYHFDPYLA"
BASE COUNT         9844 a         7033 c         6162 g         9500 t
ORIGIN      
        1 gagcaacttt atgcattaga agattttgtt aaatcgattc atttagaatc tcaattaaga
       61 aaagttggat aaaaaaatgt tagagcaaca acctacacaa aacccattaa cagtaacaat
      121 gttaaatgca caagcgcagc aggttaataa accacttcgc gataatgtga aagcagcatt
      181 aaaaaactat ttatctcaat taaatggtga agatccaaca gaattatacg aattagtatt
      241 atctgaaatc gaacacccaa tgttagatat ggtgatgcaa tatactcgcg gcaaccaaac
      301 ccgtgctgcc acaatgctag gcattaaccg tggcacatta cgcaaaaaat taaaaaaata
      361 cggtatgggt tagtctaacc gaccgttaaa acacgatttt aattcgtatg taattagtta
      421 gagccaacaa aaaacgctga atatactatt cagcgttttt ctattttatt ttgccgtcta
      481 aaacttcatt tcaaatgcta aactgaaatt tcgccctggt gcggcaaatc gactgtaatt
      541 gctacctcgg tcttggttta ccgcattcac accggattga cgcactgatt cccaagtaga
      601 atatttacgg ttagtcacat tataaatgcc acctcgtaat gtatagtttt tataattgat
      661 gtaaccggtg atatcgtgag tgtaccaaga accggtaagt ttgccaccta aatcaacgcg
      721 atatatgccg tgatgacgtg tgcctaccac ctcatcggcg tttttggctt tagaataggt
      781 ggtagtaatg ccaattcccc atttttcttc tgggtgatcg tagccgaatc caagcacata
      841 gcgtgctggc tgaatcgcat ctagaatagg atcgtttacg ctatcgagtc ttgagtcgtt
      901 taatttccgc tcttttactt ttacacggtt ataagcaact gatgaataaa aaccatctgg
      961 cagcatagat aaaataccgt gtaagtcaaa tttagcgact aaatttatcc cgtttagtgc
     1021 tacatcttga atattatggt agttagagaa acagtgtgat ttttgtatta ttcttttaca
     1081 tgctgtcgca atcatatttt tatagttatt acggaaatag ctcacatcaa ggtaaccaaa
     1141 ttgaccttta aagctaactc caatctcatg attagtcgat ttttctggct ctaactggtg
     1201 gctacgttgc tcgcgttgca catattcgtt atctttaagc ccaatatgat cacgtttacc
     1261 ataaagttca tagaatgcag gcactctaaa accgtttgaa atgcggtaag aaagcgacat
     1321 aaactcagtc ggtttaagcg taatcccgcc attccaagac caatttcgat aagttcggct
     1381 aagtgtccac gggtcatccg atttaaatcg atgttgatca aaacgcaccc ctaatcccaa
     1441 atccacatac tcgctcgtta tcagatcgcg gaagctaaca aaatggctac cgcctttaat
     1501 tttacgcggt tcacagttta tgatgtcgcc gtgtgtatta ttacacaaag ataccgtttc
     1561 aatactgcgt ggattgcgac gataaatata tgggtcatct aaacgccctc taccgcggta
     1621 gctggtataa ccgcctttgg tatattgggc agttatatcc ccatgatcca taagcgaatt
     1681 aaatcgatca aagcccaacc ctaaattcag tttgtgggtt tgcttaaata cgccttgacc
     1741 agcatttagc gctttatcaa attctaaatg aatgacacga tgcttttctt ggtaattatt
     1801 acgctcagtt cgatacatcg accaagattt atccaaagtc ggacggcaat ttttatctac
     1861 cacaggataa tcgctacaat gcaagcgatg tagccggcta tatagttcaa tatcttgttt
     1921 atccgcactg agtttaatgc tatccaacca gcgattattc tctggtttat atttataggt
     1981 gaatcctaaa cgacgtttgt ggtgatgttc atcaaaaaac ttcacatggc tataacgcaa
     2041 cccataaggg atccttttat tgaacactaa gccatctaaa atattatccc cttcataaac
     2101 agaagagttc ttaagtgata agttgtctac cctgatatta atatcgtctt ttgtatagta
     2161 agctggcgtt tgcatatcac ggatatcgta gcgttgtttt gtatcttcta agattgcgcc
     2221 aagataatga gacgagttaa agtgatagcc aaacttcata aaaacagaat tactcttgta
     2281 atctaaaggg tttggtgcaa tgcgatcttt accggtatat tcttgggctg agagctgctc
     2341 agtgcgataa ggaatctgct caagctgttt gcgctcttca ggcgtatatt ccggaaatgt
     2401 tctcactggg aaattatcgc ggttcaactt aggccttgca cgaggagtac aatctaatgt
     2461 tgggcaagta tcttccacaa taaaaaaaga tcctccagga gcattctgtg cggaatctgg
     2521 gtaaagatcg tagcgatttt caaagccggt tatacgccga atattatgtt ttaatttatt
     2581 tgcctcggaa tgaattttgg tctctttacc gtgtcggtga gttgcaataa caagtgcttc
     2641 aaaaccaccc gcctcaccag ccgctgcgat agactgtaaa aaatggctat ttttgctggc
     2701 ataagaggtc ttactatcta agccccaatg ctgcccctct ttaataatat cctgcgcatc
     2761 tttagtacga aaaccaatag caccacctag cgcaccagag ccatattccg cagaacttgc
     2821 tcctttgctt aactcaattg aacgaatgtt ttcatactca atctcattaa ttgcaccacc
     2881 attagcatct gaacctagcg tatgataatg ctgcgcttgt ggtagcccat caacaagtaa
     2941 gctgacacgg tttttatcta cacctcgaat ggcatagcca ctacttgcac cgcgaccttg
     3001 ttccaccacc gaaataccag ggtcatagcg agttaaatca cgaataccaa gaatttgatt
     3061 tttactcatt tcgtgataat ttttcactac tttccccaag ccagttactt cgttttgacg
     3121 ttcgtgagca taatggctct ctgtcacaat aacttcatcg agaactgtag aatgatcgtt
     3181 tgtcatttga tgttcattag caaataattg agagtgtgag acggaaaaaa tgactgctat
     3241 agcaatcggc gaataatgga aagtatgttc tttcattgtt ttatttatct cctttagatt
     3301 ttacattttt ttggcgaaaa ttacccgctt gttagagcgg gtaagtcttg gcttattttc
     3361 tacctagcgt tttccaccga aagtgatact cactttatct ttattagttt ctgcactatg
     3421 cacaatgccg cctaattcag cagcttgcgg accataaaaa ccaccttcaa atttggcatt
     3481 aagatgaaca tctgtaccac cagctgtact cttattatca atattaaagc ctttttcacc
     3541 cgtttttgct gtaccgctaa aaccattgcc ttcaatcttt ccatctaatg tcagcatcgg
     3601 atttgtctct aaaccatcat ttgcaattaa tttgccctca atcttcttat cagcaaaatt
     3661 cacattaaat atagaacgag taccatgagc tttatcactt ggcgaatcgc cacctacacg
     3721 ctcgtcctta ctacgaatgc tgccattcca agtgccagta taacgataaa tacgagcctc
     3781 ttttggtaaa tcagcaactg ccgtacgttc acccactagg tattgatgcc cgtctttacc
     3841 cttttgctca aatgtaccga atttgacata gtctaaatta ttacaacaga ctgtaatttt
     3901 ataatcttta ccatttatat tttttgttaa tacttgataa aaatccatat cagcaaaacg
     3961 tgtttttcct tctggcaata aagcaatttg aacaccatca ataactaaat gagttgccag
     4021 accaaaagta tccatatctg atttagccaa atctttcaaa ctcacttgca ctgaatcaaa
     4081 tttcttttcg accttgtctt cacctttttc atcacgctta cccgcaaata caacgaaaat
     4141 tgatttgtca tctgccaaaa attttcccgc aagttcctga gctttatcgc cataaaaacc
     4201 gccttctact gtattactat ctttaccaaa gtaaggatcg tctgttttta aagcctctgc
     4261 cttaccatta aagcggtttc cgtggatatt cgcttctatc ttataatgat cagtaatttc
     4321 ctgcttgtcc tttttccttg gatttacata gccattttta gttaaagtac ctttaagcgt
     4381 tttatcgcta aaattaacct caaacttact actatgccct ataggattgt cttcctcctc
     4441 tgtatttgct tggttgtttt ctggcgtagc cccaacattc gtaccagcat cacctccttc
     4501 aaaatactta cttttatttt ttgtagatgc attagtagta aaatcccaag ttccttcata
     4561 agttgctact tgggttggga aagctttaga tggttgagtg cctaaataat aaacataccc
     4621 tgtaggacct gtttttagaa gacgaggttc ttctcttttt gtctcggctg cagcccctaa
     4681 atgaatgaaa taacccgaac gaacaaattt catctctcgc ttacggtgta atttttttcc
     4741 atcgtgggtg tgaataatgt catcttcatc ataaccgaat ttttttactc cttcctcaag
     4801 ctttttcatt tcttcttcaa gagataatgg cttgcctggc tcacccacaa ttggtgtaat
     4861 ttcaccaatc gccccatttt cctctccagt gccaccaaag aaattacgac gtggaatttt
     4921 agtttgaaat cctaaggatg gttgagccag gctttcagtc gaagatatcg ttctacgttc
     4981 tgatttaggt tgggctgttg agtctttaga agagctagaa taagatgagc ttgccttaac
     5041 tgaattggta tcaaaactac caccgtttga agtacaagcc gaaagtagta cagcattcaa
     5101 taaaataacg ctatttttta ttttaaacat aattttctcc ttaattaaaa atgattacca
     5161 ttttatttat atagaaggac tatgtaaata gctagattat atttttttag agagccatct
     5221 tttaaataat aaaaattgaa aaataacgcc catcttcctc tacactaaat gtgtggaaag
     5281 ccgcccaaat cttttagttt attcaccata gcacaaaata gctctgtggt cttttcggta
     5341 tcgtataatg ctgaatgcgc ttggtgtccg tcaaagttaa tttgggcagc ttggcaggct
     5401 ttaactaaca cggtttgccc atacataaaa ccagcaagtg ttgcggtatc aaacattgca
     5461 aaagggtgga atggatcacg tttagcgtta atccgcttga ttgctgcttg taaaaaactc
     5521 tgatcaaatg tggcattatg agccacaata atcgcacgct ggcaaccttg ttctttcatc
     5581 gccctacgca ccattttaaa catttcaggg atcgcaatat tctctgccac cgcaccacgc
     5641 agggggttat caatatcaat gccgttaaat ttaagagatt cggggttgat atttgcccct
     5701 tcaaatggct tgatatgaaa atggcatttt tgatcttcaa ctaaatagcc gttttcgtcc
     5761 attttcacag taattgccgc cagctccaat aacgcatcgg tttgagcatt tagccctgcc
     5821 gtttcaacat caatcacaac aggcaaatag ccacgaaagc gattttttaa tagattataa
     5881 tcggttggtg gtgtagtcga ttgagggata gattcagtta tattttccat tttatttcaa
     5941 cttaaaaata caagcggtct gatttgcaaa attttttaca aatccgaccg cttgtgacta
     6001 tgtgaaagga attagtttcc taatgcggct tgtgcgtttt tattttcaat aaattcgatt
     6061 ttgtaaccat cgggatcttc agcaaaggca atcacagttg ttccacccaa taccgggcct
     6121 ggctcacgcg taatattacc gcctgcggca cgcacatctt taatcgtttg ataaatatca
     6181 tcaacgccta atgcgatatg accataagct gtgcctagct cgtagctatc cacgccccag
     6241 ttgtaagtta gctcaatcac tgcactttcg ctttcatcgg catagcctaa aaatgctaac
     6301 gaatacttat actgctcgtt ctcgcttcta cgtaacagac gcatacctaa cacatcggta
     6361 taaaacttaa ttgagcgctc taaatctcca actcgtaaca ttgtatgtaa aattcgcata
     6421 ttttcttccc cctgaagtta aaaaacgccc cctattatgg cataatcttg gcttaaaatc
     6481 attttcttta caccttttgg gattatctat ggctcacgat cacgattcgc cgtcaaaact
     6541 tgaaatttca acccacgttg cactgccaat ggtattaatg ctgtttattg aaatggctct
     6601 taacttctac tctgcaccga aaaattcaat ttttatttct ccttacttat tggcgttttt
     6661 cgtgacctca ttaatcagtt ttttagtgct atggaaaggc gaaatctgcc gagggcaagc
     6721 cagccgttta agctttattt tacccttttt acttattttc gccgtaggga atttccttta
     6781 ttccataggc tttacgccaa agcataaccc aatggcggtg gcaagcattg ctgcagtttt
     6841 tgcctgctta atctacttcc gcctgccgga agatcaaacc ttatataaca cattgatgta
     6901 ttgtggtttg gctatatcaa gtattggtat tatccaatat ttagccattt attggtttga
     6961 actgccttca ttgtttaacg ggattcgagc caacaatttc gcccaagtgc tgttaggcat
     7021 tttattagcc ggctggtatt taatgttagc gaaaagccgt ttggatattt tcctcaaact
     7081 gttggtgaaa accgcccttg tggtgctgat tctcaattat tgctggacag cttttgtgct
     7141 ttatcaacat ctacaaatta tgccggatat gccattgctt tcatttagca tctattttgc
     7201 agcacaattt gtgattttat tgatgttggc gtggttatta cttggaaaaa acatcaaaaa
     7261 tccgaccgct tggacagttg ctacgctgtt agcgctttta taccctttcg ctaatgtagt
     7321 ctgatttatg aaacgggcat taaaattttt cggcattgca accgttctat ctgttgcggc
     7381 aatctactat tttttaatgc ccagccacta tcttgccacg ccgattaact ggattttgcc
     7441 gaaagcgtgg aaagttcaac tcccacaagg cttaaacctg aattggcaag gctcatcact
     7501 tgctcaattt caactctctt atcgagattg cccgctacta agtgccgata aaaccgattt
     7561 gggttggatt gagaaaaacc atcttttctg tcgataaatt ggatattgat taccgctgcc
     7621 ttgccttgtt tccacaaaat gaacacagca attccgacac ttcactcaac gcattgctgg
     7681 cggtcattcc tgatggcgaa gcgactatcc acgcccttaa ctggaaaaat ttgcctaccg
     7741 atttcaaccc aagacttcag cggctttttg agcaaacagc tattaaactc tcactttcaa
     7801 aaggtgaaat tcaggcaact gccgctcaac aagcggtcaa atttgcagca aattttgcaa
     7861 atgggcggtt gaatgccgat ttatcatatc aaccgaatga aaaagaacag cataatctta
     7921 ggctttcagc agaaattgag gacaatttta cccaactgcc gcccactatt tccgctgaat
     7981 atgattggca attatctgat gaaatcatcg ccaataaagc cttgcaaaaa gggcgctcta
     8041 ttctcagttg gcaaaaaaat gccgcacaaa agttagaagg caactggcag gttgaacttg
     8101 ccgaaagtga aaataacaaa ctggatttcc cttttctgtt tgatggggaa agcctagaaa
     8161 ttcaacaagg gcggtttgat tggcacttta cccaacaatt tccactgcaa gggtttgtta
     8221 gcaccaagct cacaccgaaa tcattcaacc aaaacggctt tttgccactc aaaacggcga
     8281 ttcgtatcag cctgctttcc caaaacagtt ggggtaaagg caatattgta atctccaatt
     8341 cagacggaga aataaccgaa aaagggctga atttaccgct tcgtttgacg ggtaatatca
     8401 aacacggcaa ttttattctt tacagtagcg tgccactgga ttttcaaggt aattttgacg
     8461 atttaagcgt aaaatttttg cctagctccc tacttcgttt gaccggaaaa gaacggtatt
     8521 taaccattga agatttacgt tttccacttg ccggaattaa aattgacaag cacggtattc
     8581 acggacgact gcacgcaatt ttaagagggc aaagccctga ttttaacaat atcgagttcc
     8641 atttagacgg acaagcacaa aattttaagg ctggagcctt agattttttc caagatccta
     8701 aagatgccaa cgcagtaaaa gacagttgga aatggcgaat ttggggagca tctatcctca
     8761 aagccttcaa cagtaaagtg aatttaagcg gcagagggca atggcataaa cagcttgtgc
     8821 ggttggaaga actcaaaggc gatttagcca ccgtaaagtt agcagataca accatttcta
     8881 aaatcacgct taacaatacg caagctattc gatttaatgt caaaaaattc acccttgacg
     8941 gagctgtaat gttgcaatca cctgagattg ccttcagtta tggcggtgtg ctacccaaac
     9001 cacgagtgaa tctcgccttt gatggtgaag tcgaaaaact acgttttaaa ggagcggtga
     9061 ataccaccga actcggccca ctcaaactgt tcgcccgccg ccaattaagc caagatgcca
     9121 gccaaattat cggcaaactt tattggccgg agcaatctgc tcaaggcttc cagccgcttt
     9181 ttccgtttcg tagccaatgg gtgatcaaca acggtacaat tcggggcgaa acggcgttta
     9241 gtgccggcag taaaaacggg ctgattgccg gtgggcattt agccattcgt aacggcggtt
     9301 tatctttacc taatggcgaa gcaaaaggga ttgaattttc gcttccctac cgctaccaaa
     9361 acggacgctt tcatttcgga gccaaaaaac cgcttgatgt aaaaattgcc caaatcaaac
     9421 tgggggattt agcattagat aacgcttcaa tgaaactcaa cggttactac ccttatacca
     9481 aagctaagcc attgaattta aatcaattat cctttgattt gtttggcggt aaactgaatg
     9541 taaaacgctt tgccttacca caaaccgaac tggcttacct gaatttagag cggattgact
     9601 ttgagcaaat tttgcaattt atgcaatatc agcaaattga tctcaaaggc aaagccaacg
     9661 ctatcttgcc gttttggtta agcggaaaac cttgctatat ttgcgatggc ttactgacac
     9721 aggcggaaac ttcctattta acctttaccc ctgaattgtt gtcagaaatg cagaaaagtg
     9781 gttacaccga gcaaattttg ttacatttag tagataaaag cacgattaac gattttcgca
     9841 gtctgattaa tgtcgggccg aaaggcgatt tagtattaga cggaaaatta aaattatctt
     9901 ccaatcaaaa taaatcaaaa gtgaatttaa actataatca ccgagaaaat ctgtttgatt
     9961 tgtggcactt aattaactat ggctcacaat ttgaacaaaa attagaaaat tacctctatc
    10021 aaaaactgga acgatgataa agaatggaat aaatatgaaa ccggccacat tttattttgc
    10081 aatattttta ctgaatttga ccgcttgtac gccgaaagta cagcttgaaa cgccaagtga
    10141 aggcattacg attaatatga atgtggtcgt tgatcaccgc attgatgtga agatggacga
    10201 gaaatcccga gcggtgatca ccaaaagtga gaacaaaacg aaaaattagc tgaagataat
    10261 catttgatga tctttaaatt gtgcataaat atcggctaat ttttcccctg tcggtagtac
    10321 taaatcaggc gttaaatcaa acatcggcac aatcacaaat tcccgatttt tcatctcgat
    10381 atgcggcacg ttcaaacgct cggtttgaat ttgctgattg ccgtacaaaa taatatccag
    10441 atctaaagtt ctctcccccc aacggcgtac acgcactctt ccctgctcat tttcaatgct
    10501 ttgcaattta tccagcaatg cagtgggcga taggtcggta ctaaattttg ctaccgcatt
    10561 gacataatca ggctgatctt gcggtccgac aggctttgag ccgtagaacg ggctgatttc
    10621 aaactcattt gccaattttt ttaatttttc gaccgcttgt ttaagctgct caagcggatt
    10681 ctcaaggttt gatcctaagg cgatatagac ggtttgcata aaatatactt acaaatataa
    10741 caataaactc tctccccttg tgggagagag accgcttatg agcgttcagc gaaataagta
    10801 gagagagggg acaagcggta acttttatta aattttttac acatttcccc tctccctgat
    10861 ttttcttctg aacgaagaaa aatcgctccc tctcccacaa gaggagaggg aaatatatta
    10921 attatccatt tactttcgat tttttcttaa aggttctacg gcgacgtgtt ttctttttct
    10981 cgctaggcac actgcctgac tggcgaaccg attttagcaa ttcagtacgc tgaccgtcat
    11041 tgctaaattg gtattcgtgc caccaagcgg aaagttcgac caaatcgcca ccttcaattt
    11101 ccgcccgcat tactaataag tcaaaccctg cacggaattt gatatgttct agggtttgtt
    11161 gcagtcggtt gccggttcgt ttcatcagtt ggaattgcaa tgcccaaata tctcgaataa
    11221 ccgaagtgtg tctgcggtgt aacgcaatcg ccttacaact ttctgccaac acatcatttg
    11281 ccgccaacat cattgcatcg tggctgttga agccaccttc atttttaagc tgttccattt
    11341 tctcccgtaa cggataccaa agtagtgcag caaataaaaa agcaggattg accggtaatt
    11401 tatcggcaat tcgctcatca gttgaattta acgctttggt aatcattctt tcggcatagg
    11461 aatcgccttt gacagtaaaa aatgccggca ataccggaaa caagaattgc catagttcat
    11521 attcacgcaa aagttgatag gttttcagcc ctgcaccggt ttgtaataat ttgagtgact
    11581 catcaaataa acgggctgcc ggaatgttgg ttaacagtgc agccatatcc cgaattgggg
    11641 aggcagtggc tttatccaaa aacatatcca acttcgccat aaagcgaacg gctcgcaaca
    11701 tacgcaccgg atcttcttgg taacgaatgg ctggctcacc aattagcgaa agtttacccg
    11761 ctcgtaagtc ttcaatgccg ttgaagaaat caaaaataag attgcgtttt atatcgtaat
    11821 ataaggcgtt tacggtgaaa tcacggcgtt cggcatcatc tcggagtgtg ccgtaaacgt
    11881 tatcacgtag gagcattcct tcatcgctga ctttagaaat tttgtcattg cgatgagatt
    11941 cgtgcccggc acggaaagtc gctacttcaa aaatttcacg cccgtaaaca atatgtgcca
    12001 agcggaaacg gcgaccaatc agacgacatt gtctgccgaa aatacgctga atttcttcag
    12061 ggcgcgcatt ggtcgcaata tcgaaatctt taggcttttt ctccaagagt agatctcgaa
    12121 tacagccacc cacaatataa gcttcataac cggcaatctg caatttatcg acaatcgaaa
    12181 tcgcattttt aggaaaatcc ttcgggctaa ttctatattg gctggcattc accgcaaatt
    12241 tcttgtgtaa caaatgggag ggaggattat cttgttgttt tttcccacct ctataagtct
    12301 tttggtccgt taagttcggg gctgggcgtt tagaacggga agttttgggt tctctttccg
    12361 ctcttttttc actctctacc ttttcatttt tctgagccga ctttttaggc ttggaaaata
    12421 atgatttaat ataattaaaa ataatacacc tctaaatagt aaatgcctct taaaaaagag
    12481 gcaccctgta aagttgatta taagcctagc tgcttttctc gaatttcagc taaagtttta
    12541 cagtcaatac ataaatcagc tgttgggcgt gcttctaaac gtttaatccc aatttctaca
    12601 ccacaagttt cgcaaaaacc gtagtcatct tcttgaattt tgtataatgt ttgctcaatc
    12661 tttttcagta acttacgctc acgatctcga ttacgtagtt ctaagctaaa ttcttcttct
    12721 tggcttgcac ggtcatttgg atcagcaaaa ttagtcatat cttcttgaag ttggcttttt
    12781 gtacgctcag cttcttccat aatttggcta tgccatgctt tcagaatttt agtgaaatgc
    12841 tcacgttggg cttcattcat atattcttcg tcttttttaa gctcataagg ctcaacgcct
    12901 gctaatgcta ataaaccgag tgaggtttta gccgcttgag tcatagatat ttactccttg
    12961 ttccctgttt attggaagtt ttattgttaa aattattgcc cgaatacaga ctaacttcct
    13021 tgtaaccgca ctatacgaaa aaatcaacat cttcgggttt taaataatgg cggctataaa
    13081 taacacaaaa acgactcatt aatcaaatga atttgcaaaa attttgcatt caaattgatt
    13141 tgattgcaca aaaaaatagc cgaacatcca ttcggctatt tttcaaacac tttttaccag
    13201 ctctttgacc aatcggctaa ccatttttgg atagtttcag tagtcggtgg cgtaaattca
    13261 ctggttttat aggttggcat tgccgcaaaa gccggatcaa caccgtcggt taaaaccgga
    13321 ttcattacgt tgtggtaaga aataatatgt tgcgattccg gtttgtgcaa aaattcaaga
    13381 aattgatttg ccaatgcttt ttgttggctg cttttgacaa tagccgcatt ttctacttgc
    13441 accaaatgcc cttcctcaaa cggagcagcc acatatttat cggtgttttc gtgccactta
    13501 tgatataaag gcgatgtagt atagcttagt accaaatccg actcgccttt taagaacgct
    13561 ccataagttt cagaccaacc tttgcctacc gttaccgtat gttttgccaa tgttttccaa
    13621 gcagttggag cttcatcacc atacacttga tttacccaca ttaagaagcc ccgcccaacg
    13681 ctgctggtac gtgggtcttg gtaaatcact tttaaatctt ggcgttccac caactctttt
    13741 aaactttttg gtggattttt cagcttctct ttatcgtaaa taaaagcata agaacccgtg
    13801 tcataaggga agaaaatttt ctgttgggtt tctaatttgg tctttgcttc aaactcggca
    13861 aacaagccgc ttttttcggc ttcaggttgc aggaaattat caataccgac caccacatca
    13921 gctttggttt ttgctccctc caaacgcaag cggttgaaca tcgttacgct atcgtcaagc
    13981 ggaacaaatt tgacatcaca gccacactct ttctcaaaat tctgctctaa tttaggagca
    14041 ggtccatatt ccgaagcaaa cgagctgtaa gtgtaaacgg ttaaggtcgg ttgattggca
    14101 taaacacttg cacttaataa cgcaagtgaa gagaggataa atttcataac aattccttct
    14161 aaattaaatg aatagaagga tttaagaaag gcaagttgct atctcaaatc cctacgctga
    14221 tattaatcag atcaggttct atgggtattt ctcagccaca agcggttaaa tttcggcaaa
    14281 aatttgcaaa tttttacgca aaaacaaccg cttgttgcac cccaatgaga acggcttcat
    14341 tgtaaaaagt ttgcaggcag atttcaacca aagcaaacgt ttgctatttg tattaaaaat
    14401 ccgtatacta ccgccctagt ccaaacaagg aactagaaat gaattgcaca acatcggtta
    14461 aatcaaattc ccaacagcta cgcttcaact ttagaggcgt agctgttttt ctattcattt
    14521 caaaccattc aaaatcgctg ttatagcgat tttttttcgc acgcttgcaa ttaccgcaag
    14581 cggttgagcc acattaacag agagaaaagg aataaaaatg agccaattga ttcgtaccct
    14641 aaaaggacat attcgtgatg aaatcatcaa aaaaggcggt tgggtaaatg cacacgccca
    14701 cgcagatcgt gcatttacga tgacgcctga aaaaatcacc gtttaccaaa atgcgaatct
    14761 acagcaaaaa tgggatttgg ttgatgaggt aaaacgtaat tcaagcgtag aagattacta
    14821 ccgccgcttt tcccaagcga ttgaattgat gatttcacaa ggggtaacgg catttggtac
    14881 ttttgtggat atcgaccaag tatgtgaaga cagagccatt attgccgctc acaaagcccg
    14941 tgaagtatat aaaaacgaca tcatcttgaa atttgctaac caaacgttga aaggcgtgat
    15001 tgagcctaat gcgaaaaagt ggttcgatat cggctcggaa atggtggata ttatcggcgg
    15061 cttgccctat cgtgatgaag tcgattacgg caaaggcttg gaggcaatgg atattttaat
    15121 ggataaagcc aaatcgctcg gcaaaatgct ccacgtccac gttgatcaat ttaactcacc
    15181 taaagaaaaa gagaccgaac aactgtgtga taaatcgatt gagcatggta tgcaagggcg
    15241 agtggttgcc attcacggga tttccatcgg ctcgcacccg aaagaatatc gaaaaatgct
    15301 ttaccaaaaa atgcgtgata gccaaatgat ggtcattgcc tgtccgatgg cgtggattga
    15361 tagcggacgt aaagaagatt tacagccgtt ccacaacgct ttaacccctg ctgatgagct
    15421 gattcctgaa ggcattactg ttgctatcgg cacagataat atctgtgatt atatggtacc
    15481 gctttgcgag ggcgatatgt ggcaagaact cagcctttta tcagcaggct gtcgttttac
    15541 taacctagaa gaaatggcaa atattgccag tatcaatggt cgaaaagtac tgggtttagt
    15601 ctaactgaat aaacgaaagg cgaaccctta ggttcgcctt taataacagc ataaccgatt
    15661 atttaaacaa gttacccaaa atcccttttg ccgcttgagc taataagtca tttgcatcaa
    15721 agttttttaa gtcttcaaca ttaccattcg gggttaaagt atccacgatt tttggcaaga
    15781 actgggctaa taaatcgctc gcatcagcag catcaacacc cgcttcttga gccacatttt
    15841 gaatgttttc ttgcccgaac acattactga tttgttcgcc cgaaacaggt gcattgtctt
    15901 catcgctgct gatccaagtg cgtaacacat catctaatcc gccttgttgg aatttggcaa
    15961 tcagcccttc aatcccacct tgtgaggcga gtaaattttg aattaattga gttgcagttg
    16021 attggttatt gccactcaat actgaagaag ccaatgaacc taaaatattt cctaacataa
    16081 tttctcctat gcaagcggta caattttgca aaaaaacagc aaacttcgac cgcttgtaat
    16141 cactttaacg agctaaaatg gctcgcactc tttccaaatc ttccggcgta tccaccccca
    16201 cttgcggggc ttctttcgcc ggttcgatat gaattttttc accgtgccat aaggctctta
    16261 gttgctcaag ggactcaagc tgttctaatt gtgtcggctg ccacgccaca tattgtttca
    16321 caaaaccggc tcggtaagcg taaatgccga tatggcgtaa ataaccttga gcggcaacaa
    16381 aactgtcatc acaatccgga aaactgtctc tggcaaacgg aatgctggct cgggaaaaat
    16441 aaagtgccat tccgtttttg tctgccacca ctttcaccgc atttgggtta aatagctctt
    16501 ctcgggtcgc taatttcaca ccgagtgttg ccatattgac ttgataagca tctaaatttt
    16561 gagctacctg agtaacaatt accggtggaa tcagcggttc atcaccctga atattcacaa
    16621 taatttcgct gtcggcaatc tgcatttttt cgatcacttc cgccaaacgc tcagtgccgg
    16681 aattgtgttt ttccgaagtc atacagactt ccgccccaaa agattgagcg agggcttcaa
    16741 tttcagggtg atcggtggca ataatcacac ggcttgcacc ggcaagctga gctttttccc
    16801 aaacgtgttg aatcatcggt ttgcctgcaa tatccaacaa cggcttacga ggcaaacggc
    16861 tggaagcgta acgagcagga ataattacag tgaatttcat aggctatcct tatgcttctt
    16921 gagcatctaa gaaacgttct gcatccaggg ctgccataca acctgtgccg gcagaggtaa
    16981 tcgcttggcg atagttatga tccatcacat caccggcagc aaatacgcct tccacagaag
    17041 tggcagtagc attgccttcc aaacctgatt tcactttaat gtagccgttt tcaagttcta
    17101 attgaccttc aaacattgcc gtattcggtg cgtgcccaat cgccacaaag aaaccatcaa
    17161 ccttaatttc ttcgctcaat tcttctttgg ttgattttaa acgtaagccg gtaacgccca
    17221 tattatcgcc taacacctca tcaaccgtac ggtctgtgtg cagaataatt ttgccttctt
    17281 caatacgttt ttgcagacga ccaagcaaga ttttttcagc acggaagcta tcacggcggt
    17341 gaattaaatg cacttcactt gccaaatttg ccaaataaag tgcttcttcc acagcagtat
    17401 tcccaccacc gactaccgca accggtttat tgcgatagaa gaaaccatca caagttgcac
    17461 aagccgacac gcctttgcct ttgaaatgct cttcagaagg caaacctaaa tatttcgccg
    17521 acgcaccggt ggcaataatt aacgcatcac aggtgaaagt gtataaatct ccgtttagcg
    17581 tgaatgggcg tttagataaa tccactgaat gaatatgatc aaacacgatt tcagtttcaa
    17641 atttttcagc gtgcttgagc attttgttca ttaactctgt gccggtaatt tgttcaaatt
    17701 cccccggcca gttctcgatt tcatcggttg tagtgagctg tccgccttgt tgcataccgg
    17761 tgactaaaac cgggtttaga ttggcacggg cggcataaac agcagcggta taaccggcag
    17821 gccctgagcc taaaattaaa agtttggtgt gtttagtggt cataatgaat cctctgaata
    17881 aatattaatg tttattttag cctaaatttc agttcaactc tattttcgtt ttcgctcaac
    17941 ttttttcaat tttgtgacaa agttaacaga aatccgactg tgtttcttgt aaattgtgac
    18001 agtctaattc atttaaaagg ggcaaaaaga tgaaaaaatt aattaattca atagaaaccg
    18061 tcttagatga gcaactttct ggttttgtga aagcacattc tgatttaaca cttaacacag
    18121 aacctgtcta tatttaccgc acaacgccaa gtaatcaacc taaagtagcc cttatttccg
    18181 gtggtgggag tgggcacgag ccaatgcacg ccggttttat cggtaaagga atgttagacg
    18241 gagcttgccc cggagcaatc tttacatctc caacgccgga tcaaatgttt gaatgcggtt
    18301 tagcggttga tcgtggcgag ggtgtattgc taattattaa aaactatacc ggtgatgtgc
    18361 tgaattttga aacagccacc gagctacttg cagatagcgg tgtaaaagtc gcaaccgtgt
    18421 tggttgatga tgatgtggca gtcaaagaca gtttatatac tgccggccgc cgcggtgttg
    18481 caaatacagt attaattgaa aagctgttag gagctgcagc ggataaaggc tataacttat
    18541 ccgaattagc caaattaggg catcagttaa acaataacgg gcattctatc ggaattgcct
    18601 tatctgcttg tactgtgccg gctgccggta agccatcatt tactttagct gaaaatgaga
    18661 tggaatttgg ggtaggtatt cacggagaac cgggcattga acgtcgccct tttgaaaatc
    18721 tggatagtac cgtgaaacaa atgtttgaga ccttaattgc tcacggcgat tatcaacgta
    18781 aaattcgcca ttggaataca caaacccaac aatgggacga aatcgaagaa agcaaagtgg
    18841 cattgaaaaa aggtgatcgt gttattgctt tagttaataa cttaggagca gtgccactat
    18901 ccgaacttta tgctgtttat gatgcgttag aaaaagagtg ccaaactttc ggcttaagca
    18961 ttgaacgcag tcttgttgga tcatattgca cctcgctgga catgcaaggt atctctatca
    19021 cattactgaa agtggatgag gatattctca aactatggga tgctccggta aatacaccgg
    19081 caatgcgttg gggtgaataa gattgaaagg ataagttatg acaatatcaa aacaacagat
    19141 cttaaaatgg cttgaaaatt gccaacaggt aatgactgaa aaacgggatt atttaaccga
    19201 attggatacc gttattggcg atggcgatca cggtttaaat atgcagcgtg gatttagtaa
    19261 agcacttgaa aaagcccata ccgtgcaaga taaagatatc ggcaccctgc ttaaaaccgt
    19321 tggaatgaca ttattatcgc aagtaggggg agcgagcggc cctctctatg gttcattttt
    19381 tattaaagcg gctcaaggtg ctacaggcaa agagcaactg agctttaacg agttactggc
    19441 acttctacag caaggagcag aagctatcgt acaacgtgga cacgcacaag ttggcgataa
    19501 aacgatgtgt gatttatggc tgccattact cgaagactta gctcaagccg atagcactca
    19561 accaattgct caaatcttat tacaagcggt cgaaaaagca gaaaattttg caaatcaaac
    19621 cgctccatta atggcgaaaa aagggcgtgc cagttacttg ggcgaacgca gtatcgggca
    19681 taaagatccg ggagcaacgt ccagctacta tatttttaaa gccttagctg aagcattaca
    19741 gtaggagggg taaatggtta atttagtgat tgtttcacat agtgctaaac tggcggaagg
    19801 cgtgggggaa attttggctc aaatggcaca ggggggctgt aaaatcgccc ttgccggcgg
    19861 cattgaggat acctcaaatc ctatcggcac tgatgcggta aaaataatgc aagcgattga
    19921 atcggtattt agcccagacg gtgtgattgt gtttgtcgat ttaggcagtg caattttaag
    19981 tacccaaacc gccatagatt tactggagcc ggaacttgcc gaaaaagtga tcattagcta
    20041 cgctccttta gttgaaggtg cttttgctgc tgtagttgca gcctctgccg gcgatagttt
    20101 agaagcggta ttaaatgaag cgaacgaagc cgctgctctt aagcaggaac aagccaatta
    20161 gtaaaaatgc cgtatcgaaa aatacggcat tttagtttta ttagtacaat agtgaataaa
    20221 gctgtcttct aaatttcggc acaatcgggt ctgtacccag tgcagataag attgccagaa
    20281 attggctttt tacctcgccg ttcgctacat ttaaatccga ttttaaccaa tcaaaaagta
    20341 acgtgagtgc ttcttcattt ttatgagctt ggtgtagctg atttgccaat ttaatcgcaa
    20401 tttcaggggt tttgctgttc gcataatccg cttgaagctg ttgtaattcg ggcgattcgc
    20461 tggcttgttc caataattcg atttgggctt gcaaaccgtg ccaacggctg tctctgtctt
    20521 gaatcgggat cgctttaagg atttcacgag cctcttccac gcttttcatt gcaatgtaag
    20581 tttcggcata gagcaaagcg aaatcgctgt ttttcttatc ggtcatttcc cacgctgatt
    20641 tcagcagcgg caaggcttcg gtataacgct gatcttgcaa taattccaag gcttgttgaa
    20701 atttcagctc ttcctctttc ggtaaaatat ttgccaaccg aatccgtaat tcctgctcac
    20761 ttactgcacc ttgaattgca tcaatcggct gaccgccggc aaacaaatag agcgttggaa
    20821 tttgttgaat gcgaaattga gcagccaatg cttgttgctc atcacaattt accgtcgcca
    20881 ataaaaattg gttagggttt tcatccgaaa tacgacgtag taaggcatcc atttccaatg
    20941 aagccacttc ccttggcgaa ataaaatgaa acaccgtcgg cacttgtgcc gctgctgccc
    21001 atacttcact aaaattctgc tcgtttacat ttacaaagta gtccatacaa aaccttctta
    21061 agctaaaaat tactttttat attgtagcat taataagaag tttcaatagg tagcccgttt
    21121 ctctcccacg cttcaaaacc accttttaca ctataaactt tctcaaaccc ttgttcgatt
    21181 aaaaactgtg ctgtactttg gctactcact ccgtgatagc aaatcacaac caccggttcg
    21241 tcataatcga cctcgtctaa aaacttacca taactctggt tggttaaatg aaacgcatcc
    21301 tgtgggtggc tgtaaacata acggcgggca tcacgcacat cggcaagcac cgcaccttcg
    21361 ttttcgatta attcccaagc ctgttgcggg ctaatttctt taaatgtagt ttccataatc
    21421 actttccctc aaaaaacgtg caactatacc ataaaattct aaaaagaatg gctattattc
    21481 caagttgaaa atttgatgtt tgtcagatat aattgcaaac tattttcaaa tgcactgatt
    21541 taaaactacc acagtttgag gattttatgg agcaattatt tcaattttta gaacatcata
    21601 ctgactttgt tatattagga ttactaggct tattaagcgt aatctgcctt tggaaagcta
    21661 ttgaacgtat tatgttttat tccaaagtag atatttcaaa atacgatacc ctcgaagaat
    21721 tagacatcga tttaacccac aatttaacta ccatttcgac tatcggctct aatgcccctt
    21781 atatcggctt attaggaacc gttgtcggta ttcttttaac tttctatcat ttaggtcaat
    21841 caggtggtga tattgatgcg gcctctatta tggtaaacct ttctttagct ctaaaagcca
    21901 ctgctgtcgg tatcttagtg gcaattattg caatggtttt ctacaatggt tttggtcgta
    21961 aagtggaaga aaacaaactg aaatggttgg cattacaatc aaaaaagcct catcaagcgg
    22021 tgtgatttag tccatttttt gcaaattaat aaggcagaac aatgaaaaaa ttcgatgaaa
    22081 tcaacatcat tccatttatt gatattatgt tggtattact ggctatcgtg ttagttaccg
    22141 cctctttcat ttctcagggt aaaatccaag taaatgtacc gaaagcaagt acaacacagc
    22201 caatgaaagc agacgattta gcgaagcttt taaccattac cgaaaataat gagtttttct
    22261 ttaatgatca agcaataact aaagagcaat taattgctga agttgccact tgggataaaa
    22321 gccaaaaagt gtcactcaaa gttgatggtg cagtcgcctt cgaaaaattt gtggaattaa
    22381 ccgatatcct ttctgcaaat gaaattaaaa atgtggcaat cattactaaa aaagaaactg
    22441 ctccagcacc ttcaagcaca cctggtagcc ctgcacaagt gccagcagtc gcaccttaaa
    22501 tagggtaaac aggtatgaaa aaaaaacatt ctcgtatcgg cttattggca tctttggcta
    22561 ttcacactgc cttaattggt ggcacttttg cttggttaca taaccacaaa gtggaaaaaa
    22621 ttgccgagca aaactctctt tctatggaaa tggtggcagc attattagag caacctcagg
    22681 ttgccgttgc tccggatcct attaccgaag aggtcaaaga aatcgaaccg gagaaggcag
    22741 aagtaccacc tgaacctaag cttactccgg agccaactcc agagccggag gcaattccag
    22801 atcctacgct aaatccgaaa aaggaaaaac cgaaagaaga aaagcgtaaa gagcgacacc
    22861 accataaaca taagcatcgt cacaaagaga aaagggaaga agaaaaaccg aaagataagc
    22921 ctaaagagcg cccgaaacat catcacaagc atattaaagc attagagcgc gggccagaga
    22981 taaaacaagg tattgtcgcc aaagcgattc caaatgcggc tgaaggtgaa aaagtggttg
    23041 cgggggttgt tggagggcaa aaaaacggtt caccaaattc aacctcaact acaggttcag
    23101 caaatactgg ggctagcagt ggtaatgcca gtgaacttgc tgcttataaa gtcgcattac
    23161 agcgagcatt acagcgtaag gcgaataact cttaccctca gcgtgaaaaa atgatgcgga
    23221 aaaccggtac agttacgatt aaatttaccg ttacatcgtc cggttcaatt acaaatgtat
    23281 ccgtactcaa ttcatcggga aatagtaatt tagataacgc tgcggtacgc agtgcagaag
    23341 gtttgagtat gcctccgcca ccggcaggct tccctcctac ggtaacagta ccggtaagat
    23401 ttgcccttga ataaaaacag ccctcaaaag caatgtactt ttgagggctt tttattgcaa
    23461 aaaattgact aaaaatgacc gcttactgct tgcgaatatc gccatttctc acattcggac
    23521 tcacttcacc cgtacgagtt tctgtgctta acgaggtaga atttactttc gtaaaagtat
    23581 aatccgcttt gctgaattca cctgtttgat tatttaagaa gaaattgcgg tactctttta
    23641 cccataatgc cgttgcgcca caaactgcca caggcataac tactaaattc acaaatggaa
    23701 tcatcgtgaa taggctcact aaagcaccaa aggtaaaatt taaagtgcga gactgcgaca
    23761 acgcatttcg catacgctga aaactgattt tatggttatc aaacggatag tcacaatatt
    23821 gaatggcgat caaccaagca ccgaagataa aagtcagcac aggtgctacg gtttgcccaa
    23881 ccacaggcaa aaagcctaat acaaaaagag caatcagcct tggaatgcta tacatcattt
    23941 tttgccattc tcgtttcagc attcgaggca catcttttaa cattgcaccg aatgacatat
    24001 cgcccaaagg ctctccggta agttgcagtt cgactttttc tgctaataac gcattaaagg
    24061 gagcagcaac aaagttagcc aaggtagtaa aagcaaaata aaatcccact aatatcatca
    24121 aaaaaatcag tggaatcagt acaaagctaa gccattccag ccagcttggc agtaacatat
    24181 ccaacaagcc accgatattg gcaaaaaatc cccaaatcag ccctaccatc agcacggtat
    24241 taataagaat tgggaaaatc acaaaaggca aaaattgacg ttgtaacaat aaccgccagc
    24301 catatataaa ataatgaaag cctaatccga cttgattttg cggctcagaa aaaactgaag
    24361 acataatgat tccttatttt ctatcaaaat taattagaaa tatggtaagc tacgcacatt
    24421 attttcgcaa atatgaaaat acatttttta ttgattattt gataaagagg acacaatgga
    24481 actgcatatt atttttttta ttcttgccgg gctactgatt gctgtactaa ttggatttag
    24541 cctttggtcc gctcgtcgtg aaaaatcaaa tattttttca aatacattct cgaccagacc
    24601 tattgctgtg ccaccacaga ataataaaat gagcaatagc atcccaacct cattacagcc
    24661 acaaaatgag gattatcaag caatgtctgt gccggaagaa aatccgcaac agatacaaca
    24721 ggaagttgaa agctcgctgc aaaatatcaa aatcagtttg ccgggcggaa atcctcagca
    24781 acagcctgag caaattcagc cgatacaatc ggtagcagaa ccaacagtta gcacttacga
    24841 tcagaaaatt tctaatcagc aacatatcca atacaatacg ccggattttg caaacccaat
    24901 ggtgaacaca gtggtggaag aaacacctca agcagtagct atcgaacctc aagaaaccca
    24961 atcggcagag gaagaaaatc catttgtaac cctctatctt tacgcaccac aaaatgaagc
    25021 tttccgcggt gattttgtag tacattattt agaagaagtt ggcttacgtt ttggcgaaca
    25081 ccaaattttc caccgccatc aacatattga tgattcatta agtccgatta ttttcagtgc
    25141 agcaaattta atgaacccag gaacctttga tttaaataat atctcaaact ttgcgacagc
    25201 tggtttagtg ctatttatgc aacttccaac cgaaggcaat gatgtggcta atatgaagtt
    25261 gttaatcagt aacgctgaac acttagcagc atcgttaggg ggattcttat atgacgataa
    25321 tcaacagcct tttggcgaag aatcccgcca acgttatatg caacgtgtga tgcactaatt
    25381 gcgaaaaaat taataaatcc gaccgcttgt acaagcggtc ttttttgtag agaattttac
    25441 caatgtcttt atttaaccaa acctctttat ttgaagagaa tgccacgccc gagttagtcg
    25501 agaaaatcga aactattcgc caaacattgc gtcaatacga gtatgaatat cacgttttag
    25561 ataacccaag cgtacccgat gccgaatacg accgtttaat gaacgagctg aaaaatctgg
    25621 aatggcagca cccggaactg attagtgccg actcgccgac tcaacgggtt ggtgccaaac
    25681 cgctggcagg ctttgctcaa gtcacccacg aaatccctat gctttcgttg gataatgcct
    25741 tctccgatga agacttagac ggctttttac gccgtatgga aagctatatc accgcctctc
    25801 cttcgcaact ggaattttgt tgcgaaccta aattagacgg cttggcagtg agtattttat
    25861 acgaaaacgg tatacttacc cgagctgcaa cccgtggcga tggcacaacc ggcgaagaaa
    25921 ttaccgctaa tatccgcacc attcgcaata ttccgttaaa gctcaacacg actaatccac
    25981 ctgctcgatt agaggtgcga ggcgaagtct ttatgccaca atcaggcttt aatgagctga
    26041 atgagaaagc cttagctaaa ggcgaaaaaa cctttgccaa ccctcgcaat gccgcagcag
    26101 gttcgcttcg ccagttagat ccgaaaatca cccgccagcg tccgttagtg ctgaatgcct
    26161 acggaattgg ggtgtatgaa agcgatgacg aactgccagc cacccatttc gaacgtttgc
    26221 aatggctgaa atcgcttggt attcctgtaa ataatgaaat ccgcttggtt caaggcaaag
    26281 ctgctctctt ggcttattat gcggagattc aggctaaacg cccaacatta ggctatgata
    26341 ttgatggcac agtattgaaa gtaaatgaca ttgccctaca agagcaactc gggtttatct
    26401 ctcgctcctc tcgttgggcg attgcgtata aatttccggc tcaagaagaa atgacaatcc
    26461 tcaaagaagt ggacttccaa gtgggaagaa caggggcaat cacccctgtg gcgaaattag
    26521 agcctgtttt tgtcgcaggc gtaacggtaa gtaacgcaac gctacacaac ggtgatgaaa
    26581 tcgagcgact tggtattgtg attggcgata cggtaattat tcgccgagcg ggcgatgtaa
    26641 ttccgcaaat tacaggcgtg gtgttagaac gccgcccgga aaatgcaaaa aaaattgaat
    26701 ttccgaccgc ttgtcctatc tgtcactccg ctgttactcg ggtagaaggc gaggcagtag
    26761 cacgctgtac cggtggttta ttctgcgaag ctcaacgtaa agaagccttg aaacatttcg
    26821 tttcacgcaa ggcaatggat attgacggcg taggcgaaaa actaatcgag cagttaatgg
    26881 ctcgagagct gattgaaacg cctgccgatc tgtttaagct cgatcacact accctaatgc
    26941 gattagagcg aatgggggaa aaatcggctc aaaatgctct caacagcatc gaaaaagcaa
    27001 aaaacactac tcttcctcgc tttttattct cgttaggtat tcgtgaagtg ggcgaggcaa
    27061 ccgctctcaa cttagccaac cattttggta gcttagaggc gatccgccaa gccacttttg
    27121 agcaattaaa agaagtgcaa gatgtgggcg aggttgttgc caaccgtatt gtcagcttct
    27181 ggcaagaacc gcataatgtg gaagtggtgg aagatttgat cgctcaaggt gtacgttggg
    27241 aaaacgttgt gaaagccgaa attgtagata acccgttaaa agataaaaac gtggtcttaa
    27301 ccggcacact aacccagctc acccgtgatg aagccaaagc cctattacaa agtttgggct
    27361 gtaaagtctc cggctcggtt tccagcaaaa ccgattactt aatcgccggc gaaaaagccg
    27421 gttccaagct ggcaaaagct caagagttgg gaattcagat tttaagcgag caacaattta
    27481 ttgagcttac tcaacattaa caaacaaagg ggcagatgat tgaaatctgc cccttattct
    27541 atctattgaa tgcttaaaag cattaaccaa tcaatttaaa ctgctgagct aaaaacagta
    27601 acgtaaagct agttaagtaa attaagccga taactgccaa ccaaaataac cagccttttg
    27661 cacgatgctc accttttagc actaaagaga aatttaacca cgcaaaaaca ggggtagaaa
    27721 caaatgatga aatcattgca aattgtaaca ttttcgccac atcactcata aattggctaa
    27781 taattaaaat gccgatgagt gcagttaccg tcatccaaat acttaattct gtttgtgaat
    27841 gttgcttacg cccgctaatt aaacgcaacg cttctgcatt cgctcgtgaa tatccatcaa
    27901 taacagtgat cgttgtgccg aacatacaca taaatgcaat aaatgcgatt aaaagcttag
    27961 accagtcacc aatcgcaaaa gcatacattt tgattaattg agcaatatag gcaacaccgg
    28021 cttgttctac tttctcacct gaaccgtact gaactaacgc acctaatgct aaaaaaacaa
    28081 tcgccaaaat tgccgtgccg atataaccca cattaaaatc aaaaataccg tcctcataat
    28141 tcactttact taaacgtttt ttcgcaataa cccacattga gttaatggca gaaatttcaa
    28201 ttggtgcagg catccagccc attaatgcaa cgatgaacac aagtgctgct aaattccacg
    28261 gcgaaggctc tacgaaatct ggagcaattt caacgccacc ttttaaagca gcaatgatca
    28321 cggcagatac cgtagagatg gttaaagcaa tcataatcca tttagataat gtatctaaga
    28381 atttatattg ccctagaagt aaaatacccc aagtgaccac aatcacaata gttcctgcca
    28441 ttggaatgct taaatctaac ggaataccta aagcagaaaa aataaatttt agaatcgctg
    28501 ctgaaacaat cgccaccgca gctgtgttaa tcattgcagc aaaaacgtta aggataaaga
    28561 aaataataag ataaaacttt cctttttctt tgtagccttc caataaagtt ttaccgctag
    28621 ctaaggtata ttgcacacca aagcggaaaa acggatattt aaataaattg gctaacagaa
    28681 taataatcgc cagctgccag ccataaatcg caccagattg tgtagaggca ataatatgcg
    28741 aaccacccac tgccgcagat gccatcaaaa tccccggccc cattgcagca aatttacttt
    28801 gccaagttga atgttctatt tgttgtgtca tatagctttc cttatcacat tactatcaaa
    28861 acaagatggt ttcattctac ttataaaaac caaataaaaa cactcctcta gaaaagatgt
    28921 tcataaaata atacagatat tatgctgaat agagagtatg aaatgataag aaagcattta
    28981 taaaaatgat ttaataagaa ttaaaaacta agatagaata taaaaatggt tttaaataaa
    29041 attaaaaata aattgtaaat ttttatttac caagattttt agcaaagttt tcactaaaaa
    29101 tgaccgcttg caggcaataa aaaagtgcgt ttccgcactt tttactaaaa tgatttaatt
    29161 actcaaccaa tgctcaccgt tgccattagc tggtggatta atcggttggt tattattctt
    29221 acctggaata ataatttgag gctgatcgta catacatttg ttgttcgaca atatatcaca
    29281 gggaatagca catacggcag gggcagaaag aagacaactt taaccacctt tttgattaca
    29341 attctctaat gcattttctt cagcaagact actaacgata ccatacccaa aatcctcgaa
    29401 gccttgacca tctttataac cttgtgcaaa agcaaggcac atattactaa acgataagat
    29461 catttggcaa ttacgaccac cgttcttttc acactgttta aacgcatctt gcttcgcatt
    29521 ttctaaattt ttagacggat tatcagtcac ccacatcata ttttgttcag gtgacatata
    29581 aattgatgac cataaacgag gctgtgtttt acgctttgct aagtcctctt tactggcaat
    29641 ggttaccgct tctttatatt caccacattt aaactctttt tcgacatcac caaaaccatt
    29701 catttcacga acagaagtga ttttattatc agcgtcagca aaaagatatg tccaagtcga
    29761 tccacctgta taaaccttat ctgttgtcat aaatatagtg gttgcattac gaccaacttc
    29821 ataagtttgc aaattataca aagctagttg aataaccata aacatagctt aattcattat
    29881 cttttacgca ttgaaccgct gtatcaggta aacgtttacg tgcaaaaaat tcagatacat
    29941 ctttaccacg ccaataaaca ctatctagtg gcttaatcat tgtagaacaa gcggtcaaaa
    30001 ataatgacaa aataacaaat agcaaagaac ttattttgaa attcataata attacctcaa
    30061 aagaaaaaag agcaagcttt cacttgctct ttttatgtta cttagaatag cgttggaaca
    30121 ctaaacacgc attagtacca ccaaagccga agctgtttga catcacggtt gttaatgctt
    30181 tgtcttggcg ttcagtcaca atattcatac cttgtgcttg ttcatcaagg gtttcaatat
    30241 taatgcttgg tgcaataaaa ttattatcta acattaataa tgaataaatt gcctcgtgcg
    30301 cacctgctgc acctaatgag tgtccggtca ttgatttggt tgatgaaatt gctggcgttt
    30361 tatcaccaaa gacattacgg atcgccccta actcttttac atcacccact ggagtagaag
    30421 tgccgtgtac gttaatatac tcgatttcac catttacagt tgccatcgcc tgtctcatac
    30481 aacgctcagc accttcacca cttggcgcga ccatatcgta accgtctgag gttgccccat
    30541 agcccacaat ttcagcatag attttggcac cacgagcaag agcgtgttct aactcttcca
    30601 ccacaaccac tgcaccacca ccggcaatca caaaaccgtc acgattagca tcataagcac
    30661 ggctagcttt tgttggtgtt tcattatatt tagttgaaac agcacccatt gcatcaaact
    30721 cggttgcaca ttcccaagaa agttcttctg caccacctgc aaacaccata tcttgtttac
    30781 ctaattggat taactccatc gcgtgtccaa tacagtgtgc tgaagtcgca caagctgagc
    30841 tgattgaata gtttacacca cgaattttat aaggtgttgc caagcatgct gacacacttg
    30901 atgccattgt tttggttact gcgtaaggcc caacggcttt cacaccacgt gggccacgta
    30961 ctgcatcaca agctacaagt tgactgtgcg ccgagcctgt accagctccg atcaccaaac
    31021 cggtacgatc gtttgaaact tgttcttcgg ttaagcctga gtcttcgatt gcttctttca
    31081 ttgaaagata agcgtaagcc gcagcatctc ccataaaacg atagatttta cggtcaatta
    31141 actcagccgg atttaattta atcgtacctg ccacttggct acgcataccc acttcagcaa
    31201 actcaggcac aaattcaatg cctgatttac ctgcttttag tgattccaac acttcttctt
    31261 tgttgttacc gatactggaa attacaccta atcctgtaat aacgactctt ttcattttgt
    31321 ttccttcatc attttgatct gaaaaatact tgattcagct tacacttgtt cactcaaata
    31381 agctactaaa acttgattat tttacacaat ttcattactg gtctgaactc tttctatcaa
    31441 gatagcggtt gaatttcaga cattttttgc aaaataggga atgaaattta atttcccgat
    31501 tttttaccct attacataat aatcactttt ccgctaaaat gctaaacagt tcacacttta
    31561 gtaaggaaag ctgacgtgaa aacattaacc ctgacagcca acaaaataac agccgtagaa
    31621 ggcgctgcca ttggggcgaa ccaggggagc ttcgcccaaa aattaaactc tactaaagca
    31681 cgaattgatc aacttaatgc ataccgaata gaacgagcta tcaatgctaa taatcatcat
    31741 tttatgcatg ttttctctct cattccattg cttattcatc ttaatcaccc tgtgttaccg
    31801 gcttatgttg aaaatgcacc acaagggatt tggcattttc aactctctga ttatcaaaaa
    31861 caatttctgg tttcttaccg ttttgatgaa gcaatctctg ccgatcattt gggcccccat
    31921 cccagctttg atgctcttta ttcgatgggt agcaccggta ctattaccca aacaagctgt
    31981 tctgatttag atttatggct atgctatccg aaccatttta caattgaaca atataagtta
    32041 atggagctta aaattaataa attgcaagaa tgggcaaaga cattccaaat caacatcaat
    32101 atttatttaa tgaacccaga acaatttaag agccaaactt ataacagtag cgtaaccgat
    32161 gaacacagtg gttcagcaca gcattttttt ctattagatg aattttatcg ttcagctatt
    32221 ttacttgccg gtaagcgttt actctggttg catttacata aaaatgaata ccaaaatgtt
    32281 cacaataacc ctgaaattga cttaacggaa tggattgatt tcggcgattt ttccagcctt
    32341 tcaaccagtg aattttttgg tgcaagctta tggcagctct acaaaggaat taataaccct
    32401 tataaatcgg caattaaaat tttattgctc gaatgctacg ctcacactta tcctaaaaca
    32461 aagctcatct ccaaagaatt taagaaaaaa ctactaagtg acaatgcttt ggaatatcac
    32521 tttgatccct acttagcca
//
LOCUS       DANJWC010000007        32527 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.007, whole genome shotgun sequence.
ACCESSION   DANJWC010000007 DANJWC010000000
VERSION     DANJWC010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 32527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32527
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.007"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            110..1072
                     /locus_tag="PWX02_000214"
     CDS             110..1072
                     /locus_tag="PWX02_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HDL1728274.1"
                     /translation="MKKLVKLSFLASLVMGTASVQAAETFVTIGTGGQTGVYYVVGQS
                     ICQLVNRDSAKTGLKCNAPSTGASVANLNAIAAKEMDMGIAQSDWQYHAYNGSSSFEG
                     KKNDKIRAIFSLHPEPFTVMARTDSGIKSFDDLKAKRVNVGDPGSGTRATMNVILAAK
                     GWTDKDFKVASELKPAEMASVMCDNNLDAITYNVGHPNGALKEAAASCDAKLVPVTGP
                     EIDKLVADHSYYAKATIPGGLYKGSDEPTETFGVYATLVTSADVDADKVYNVTKAVFD
                     NFDRFKRLHPAFEHLKQEEMIKNALSAPLHEGAIRYYKEKGWIK"
     gene            1274..3910
                     /locus_tag="PWX02_000215"
     CDS             1274..3910
                     /locus_tag="PWX02_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HDL1728275.1"
                     /translation="MSVESKVDYDDLQDMVASNDSGGRTPSGLAKKAIVYVAILWSVF
                     QIYYASPLPFVFQEWLTHAGINLNVVVDDTKARSIHLAFAMFLAFLSYPAFASSPKHH
                     IPKIDWVFAIVGAGLALYYIFFYEGLVKRFGAPNTQDIIAGCLGILLVLEATRRSLGL
                     PLVIIAIVFLIYNFFGQYFPADWIVSHRSGSLSQIINQQWITTEGVFGVALGVSTKYV
                     FLFVLFGALLDKAGAGNYFIKTAFAYLGHLRGGPAKAAVVSSALTGLISGSSIANVVT
                     TGTFTIPMMKRVGFTKEKAGAVEVASSVNGQIMPPVMGAAAFLMIEYVNMPYSQLITH
                     AFLPALISYIALVYIVHLEACKMNLQGLPRAEEPSPILVFLLRTVASILVIVGLAIGV
                     YYGLGWIRDVAEDYAFMIVCAFLGLWYLLAIRRVASYPDLEADDPNSEIVSLPKRKPT
                     VNAGLHYLIPVVVLLWCLMVEMLSPGLSAFWGTITLIFIQLTQRPLLNFFRKESVTKE
                     IIKQGVRDVVDSLEAGGRNMTGIALATATAGIIVGVVALTGFGVQLSSVIELLSMGNI
                     VLMLILVAVFSLILGMGLPTTANYIVVSSLMATVIMEVGRQNGLIVPLIAVHLFVFYF
                     GIMADVTPPVGLASFAAAAISGGSPINTGMVAFKYSLRTVILPFLFIFNTDLLLIDLR
                     YPGHGWVVFITATVGVLAFTSATMQYIVTKNKWWETALLVVAAFALFRPGFFMDTERH
                     IEPVQFEEQLLASKVGQNVTLKVEGLNPYGKEIEFYAQLPVPEGATGEERLKNLGLTL
                     IQTDEKIKVDGVETPKVVIDMVEMDSPAAKAGLNWDQTIHYIAVPQDAPSKDWIFIPA
                     LLLVFGIVANQRRRVKKQCVIA"
     gene            4003..4428
                     /locus_tag="PWX02_000216"
     CDS             4003..4428
                     /locus_tag="PWX02_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL1728276.1"
                     /translation="MYNKLLIAIDLADLKSAKYVVDTALQMTAKNPNAVYRVVSIIEP
                     VDNSLISAFLPKNFDKEVVAEANQKLHEFTKNHFPEGSKVQHIVAYGTIYEEICRIAD
                     EKSVDLIMMLASSKPNAKGLSSNTVKVARNTTKPILVLR"
     gene            4480..5811
                     /gene="rsmB"
                     /locus_tag="PWX02_000217"
     CDS             4480..5811
                     /gene="rsmB"
                     /locus_tag="PWX02_000217"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDL1728277.1"
                     /translation="MKKQSFKALSSRAISATIILQVLDQGKSLSTLIPDAQKQLEPKD
                     LPLVQEITFGVCRVLPRLESIIKLLVEKPLKGKTRLVHCLLLVGLYQLLYMRVPAHAA
                     VDEVVNATKTLKLDSFRALTNGVLRRFLREQEEILAKVDKHWQTLHPEWLVNKLKKAY
                     PNWREIVEANNQRPPMWIRVNQQHIKTNDYAALISELVEPEFAKNSENPTACVPKCAL
                     LLERAVNVNQLPHFEQGWATVQDAHAQWSAELLGAENGETVLDACAAPGGKTTHILEK
                     APKAKVMALDIEESRLSRVRENLVRLGQIAQVICGDASKPEEWLEEGVLFDRILLDAP
                     CSATGVIRRHPDIKWLRKEGDIAELATLQGEILKALWQRLKPNGILLYATCSVLPQEN
                     SEQIQRFLQDTPNAKQLEIDFNGEKVLEKQFFPQPNGGDGFFYAKLQKVAE"
     gene            5811..7187
                     /gene="trkA"
                     /locus_tag="PWX02_000218"
     CDS             5811..7187
                     /gene="trkA"
                     /locus_tag="PWX02_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HDL1728278.1"
                     /translation="MKVIILGAGQVGSTLAENLVSEDNDIVLVDNDPTRLDKLKDKHD
                     LQVIRGEYASPQTLRDAGAGDADLLVAVTNSDEVNMIACQIAYTLFNVPTKIARICSA
                     DYVRERERLFNDDVLPIDHIIAPEILVKEDILRLIHYPGALQITHFADELVSIVNVKA
                     YYGGPLVGYPISALRDHLPHIDARVVAIFRQDKAIVPQGSTIIEAGDEVFFICATQHI
                     RAVMSELQRLDRPHKRIMIVGGGSIGSSLARDLEDQYRVKIIERNPVRAEKLAEKLSK
                     TMVLTGDASDEELLFEEHIENIDLFLAVTSDDEANIMSALLAKRLGAKKVIILVQRPA
                     YLHLIQGGTIDIAISPQQATISALASYVRKGDILQVSSLKLGVAGAVEIIAHGDATTS
                     KVVGRQIRELKLPQGAIVGAVVRGEEVIIAHKSTMIAENDRVIIFVNDKKQIDEIEKL
                     FQLGVFFL"
     gene            complement(7320..7967)
                     /locus_tag="PWX02_000219"
     CDS             complement(7320..7967)
                     /locus_tag="PWX02_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HDL1728279.1"
                     /translation="MSIFQQTNPNRRRYGLAVFIGIIAGIISAFVKWGAEHPFPPRSP
                     IDLFTAACPQPVLDAMNAAADQVAAKTIALQECSRAFLNPPHVFLRDYIGIDPTEAAF
                     TFADHAFNWIGVTHMIFSLVFAIGYCIVAEIFPKIKFWQGIGAGIIANICVHYITFPA
                     LGLTPPVAEWPLYEHISELVGHIFWFWTIEVIRRDLRNRITREPDAEVPLDQPFR"
     gene            complement(8223..9668)
                     /gene="aldA"
                     /locus_tag="PWX02_000220"
     CDS             complement(8223..9668)
                     /gene="aldA"
                     /locus_tag="PWX02_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDL1728280.1"
                     /translation="MMKQHKMYINGEFVPALDGRTLAVYNPATEKAISEVPRGGADDA
                     KIAIDAAEAAQEAWERLPAIERGKYLRQIAVGIRERADEIARTISEEMGKTLSLATVE
                     VHFTADYLDYMAEWARRYEGEIIQSDRPNEHIFLYKKAIGVTTGILPWNFPFFLIARK
                     AAPALVTGNTIVIKPSEDAPNNAILFSEIVHQVGLPKGVFNVVTGYGNEVGPELSGNE
                     KIGMVSFTGSQFAGQKVMEAASKNIIKVNLELGGKAPAIVLDDADLELAATAVVNSRI
                     INSGQVCNCAERIYVQSSIKEQFVQKLVEKMQAVKYGNPLEQQDIDMGPMVNEKGLHK
                     VQEMVTHALHQGGNLVLGGNVVEGTGYYFEPTIIDNVDNSMDIMRHEIFGPVIPVATF
                     DTIDEAIRLANDCEYGLTSSVYTQNINKAMKVISRLKFGETYVNRENFEAMQGYHAGW
                     RKSGIGGADGKHGLEEYLQTQVVYLQFDDKV"
     gene            complement(9935..11086)
                     /gene="fucO"
                     /locus_tag="PWX02_000221"
     CDS             complement(9935..11086)
                     /gene="fucO"
                     /locus_tag="PWX02_000221"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDL1728281.1"
                     /translation="MANRLILNETSYHGKGAIQNIVHEVTSRSFKKALVVTDKDLIKF
                     NVAQKVTALLDAAGLPYEIFDEVKANPAVDVIKAGVAKFKASSADYLIAIGGGSPIDS
                     AKAIGIIINNPEFSDVLSLEGVAPTHQKCVPIIAIPTTAGTAAEVTINYVITDEAKKR
                     KFVCVDVHDVPAVALVDPDMMESMPKSLAAATGMDALTHAIEGYITKAAWELTDALHL
                     KAIELIGRSLRGAVEKNPKDVEAMALGQYVAGMGFSNVGLGAVHSMAHPLSAYYDTPH
                     GIANAVLLPYVMEFNKNYTGEKYREIARAMGVKGVDEMSQEEYRNAAIAAAQQLSQDV
                     GIPPKLHQIGVKEADLAALSVDAFNDVCTGGNPRDCTAEELLEVYRIAY"
     gene            complement(11215..11466)
                     /locus_tag="PWX02_000222"
     CDS             complement(11215..11466)
                     /locus_tag="PWX02_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HDL1728282.1"
                     /translation="MSNTLTDKALVVIAALGGRENIVDVYACITRLRVEVKNTDIVEW
                     DKIKSVGALGVVTSGSVIQSIFGSDAEAYRDEIKRILNI"
     gene            complement(11522..12469)
                     /locus_tag="PWX02_000223"
     CDS             complement(11522..12469)
                     /locus_tag="PWX02_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1728283.1"
                     /translation="MKKSALFTATLFGLSTLFAGSAMAKSDEIAVIVKSANSTFWQNV
                     RKGAETAGKDIGGQYKVTFQGPEAETAIDAQVNMVDNAVNRGVAGIVLAASDPVALVP
                     AVKKAYESGIPVVLIDSGINSDGKYYQSFLATDNRAAGKLAAEKLLAKVKGGKVAVMS
                     FTQGAASAIERTGGFIDEIKAKADYKLVGPYYSDSDMTKALNQTEDVLGSNPDLAAIF
                     GANEPTAVGMARAVKQKGFAGKIVAVGFDGNSDLQNFVRDGTLDGIVVQSSYQMGYKG
                     VDTIGKLIKGEKVEKVIDTGVVYVTKENIDSEEAKGVLY"
     gene            complement(12493..13491)
                     /locus_tag="PWX02_000224"
     CDS             complement(12493..13491)
                     /locus_tag="PWX02_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1728284.1"
                     /translation="MNEKQKEMLRKMAALAGLVLLIIFFSVTNEYFFTSNNIMTVGLQ
                     TSTIALIGIGATCVILTGGIDLSTGSVVALSGVAAAMIVNAGVPVPLGMILGILVGGI
                     CGLINGILVTRMKLPPFIATLGMMMVARGLALYVTNAAPVSGMPESFANLGNGALFKI
                     VEEGANGLPKVVFAGIPYPVIIMIFITVLFTFALSKLKVGRYLYAIGSNEEAARLSGI
                     KTNIVKIYAYVASGLLSGLTGVILASRLVTAQPNGGVSYELDAIASAVVGGTSLMGGV
                     GTIPGTLIGSFIIGVLRNGLNMNGVSSFVQMIVIGLVIIVAVSLDQLRQSKKAGKK"
     gene            complement(13488..15017)
                     /locus_tag="PWX02_000225"
     CDS             complement(13488..15017)
                     /locus_tag="PWX02_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL1728285.1"
                     /translation="MQNEVILTMKNISKSFAGVNALKGVELTLRKGEVHALMGENGAG
                     KSTLMKCLLGVYQADEGEIIYKGQPVRFESVLEAQKAGISMIFQELNLIPHLTVAENI
                     FFAREPLKHGLVDKDKMVEESAKLLNFFEIDVDPNDEVRMLSVAKQQMVEIAKALSFD
                     VEVLIMDEPTSALTEKEIDKLFELVDRLRAKGVSIVYISHRMEELKRICNHITIFRDG
                     TYVSNHKFNEITMDEIITKMVGRSLDNHFPPKTAVVTNDIILSIMNAERKGVFEPLNF
                     DLRKGEILGITGLVGAKRTELARAIFGADPLDSGEIFVHENKVSIKDPSDAIKAGIAY
                     LSEDRKLNGVAVRMSIRENITMASMDKVANQIGVISYDEEEKASKTFIDKMEIKTPTI
                     EQKVQNLSGGNQQKVVIGKWLFREAKVMIFDEPTRGIDVGAKYAIYQLLDELAANGVG
                     VIVISSELPEVLGVSDRIIVMREGRMTGMLETKKTNQEEIMHYATGVKNMFAREYGVE
                     K"
     gene            complement(15079..15732)
                     /locus_tag="PWX02_000226"
     CDS             complement(15079..15732)
                     /locus_tag="PWX02_000226"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HDL1728286.1"
                     /translation="MDRKILARQIIDTCLEMTRIGLNQGTAGNVSVRYKDGMLITPTG
                     TPYEQMTEDSIVFVDGNGKHEEGKLPSSEWQFHLAVYEARPELNAVVHNHAKNCAAVS
                     ILGEPIPAIHYMIACTGTNHIPCVPYAPFGTHQLADYVREGIKHSKAILLAHHGLITA
                     DKTLEKALGIAHEVEVIAEWYLKLLATGKPIPTLNKEQMDIVLGKFKSYGSWVESEQ"
     gene            complement(15792..16223)
                     /gene="fucU"
                     /locus_tag="PWX02_000227"
     CDS             complement(15792..16223)
                     /gene="fucU"
                     /locus_tag="PWX02_000227"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HDL1728287.1"
                     /translation="MLKGIHPAISPDLLKVLAEMGHGDELVLSDAHFPAHSIHQNVIR
                     ADGLSVATLLEGISALFEFDQYVEAPLAMMQAVKGDTLDPSVEERYLNAIKKVNGTAP
                     KIERVERFAFYDRAKNAYAVVITGELAKYGNIIIKKGVTPV"
     gene            complement(16298..17731)
                     /gene="fucK"
                     /locus_tag="PWX02_000228"
     CDS             complement(16298..17731)
                     /gene="fucK"
                     /locus_tag="PWX02_000228"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HDL1728288.1"
                     /translation="MPISLIFDCGATNLRTIAINQAGEIVAAHHLPNNTQPDVENPEF
                     HIWDIEEIWSKLMTCAKQTLSQLSEQQRQEIVGISVTTFGVDGALFDKNGKQLYPIIS
                     WKCPRTLPIMENLANYLDVEALYQRNGVGHYSFNTLFKLLWLKENKPEIYQQAESFLF
                     ISSILTYRLTGVQTTDRTMAGTSMMTNIENDSWDKTVLDLLGLTENHFPPMKSAGEVV
                     GTLKGEISQELGLSGQIPVISCGHDTQFAIFGSGAGYNQPVLSSGTWEILMVRTPKAK
                     PQWQFVQNGLTIEFDSQSGYFNPGVQWVASGVMEWFGKRFFSDIANTEHYYRTMIEEA
                     AEVPAGSNGVKLVGNFDGTTAKTGSIIGLSMHITRGEIYRAGLEYMAHRLKEGLDVLQ
                     EVGHFNAESLICVGGGSKNALWNQIRADVLNRPIDVVDFPESTVLGAAMFTFTGAGVF
                     ENPNAAQQAMKPNVKRVEPSSNSQFYK"
     gene            complement(17925..19691)
                     /gene="fucI"
                     /locus_tag="PWX02_000229"
     CDS             complement(17925..19691)
                     /gene="fucI"
                     /locus_tag="PWX02_000229"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005619331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HDL1728289.1"
                     /translation="MTTLKSTPVKIGIRPTIDGRRLGVRESLEDQTMNMAKAVAQLIQ
                     SEVRHPNGDFVECVIADSTIGGVAEAAACAEKFKRENVGAVITVTPCWCYGSETIDMD
                     PHMPKAIWGFNGTERPGAVYLAAALAGHSQLGLPAFSIYGTEVQEADDQSIPDDVREK
                     LLRFARAGLAVATLRGKSYLSIGSVSMGIAGSIVNQQFFQEYLGMRNEYVDMTEIKRR
                     LDRHIYDAEEFKLAMSWVKTYCKEGIDVNAPENQRSPEEREKLWEDVVKMTIIARDLM
                     VGNPRLAELGYSEEALGHNAIAAGFQGQRQWTDHLPNGDFMEATLNSTYDWNGVRAPY
                     IMATENDSLNGVNMLFGNLLTGQAQIFADVRTYWSEDQVERVTGWRPESGFIHLINSG
                     SAALDGTGQHTDRAGKPTIKPAWEVTEEDGKRCLENTCWCPAVHEYFRGGGLSSQFLT
                     KGGMPFTMHRINLIKGIGPVLQIAEGWSIDLPENVHDILNKRTNETWPTTWFVPRLTG
                     KGAFTDVYSVMANWGANHCVATFGHVGADLITLASMLRIPVCMHNVEDKDVFRPSAWN
                     GFGQDKEGQDYRACANFGPLYK"
     gene            complement(19887..20633)
                     /locus_tag="PWX02_000230"
     CDS             complement(19887..20633)
                     /locus_tag="PWX02_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL1728290.1"
                     /translation="MQQRQDKIIAYLAQFDEANVQELAEHCGVSIETIRRDLNKLDKD
                     GLLHRTHGGAVSHKKRDIGRSFTTRQRLNSEAKRSIAENVLEYLQPESVIALDASSTS
                     WNVAQRIPNIPCTVVSSSMRIIYSLSHKPHIKTIATGGIYSEKYDAFYGPLSEQLLTR
                     LKIDLAILSCTGIADGAIWESNEINISLKRKLIANSKQVFLLADHSKFERKDLFRIED
                     LSCIDKLFINQPPPDFLKNYCLAHQIEIII"
     gene            20913..23009
                     /locus_tag="PWX02_000231"
     CDS             20913..23009
                     /locus_tag="PWX02_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDL1728291.1"
                     /translation="MKNFKLLPVSAAVLSVLAANAFAETVATDAAVLDTVTVTDNQGL
                     KVQTNVVTTQKKEESTQTDLRGLLKDEPAISLGGGNGTSQYLYIRGMGQNSIDVKVDN
                     TYSDSQILYHQGRHMLDPALVGTVTVQKGAGSASAGIGQTNGAVIAKTVDALDLLKNS
                     DKNFGAKLGTGFSTNHAHNFNATLYGKGEIFDALVSGNLVRDRDYKGGKDYVNQFGTN
                     RVPYSALDKSSFLAKLGATLGDHRFVLSHSNERNEGERLVREEFDAAPVGTKGSRVTL
                     DRQAPANRRMTVQRTNLEWTGKNLGFAQEATANVYQLVQGRWSENETRNGYAGGVDYA
                     TKTKIVTHGANVNFDSALHENVLLKYGVNYRHQETKPYAKFSHQLVNQEKTDTGVYLE
                     AITAPVDKVTLTTGVRYDHFKFKAMDGKKRSEGAFNPSVGIIYEPIQHLSFSASHNYA
                     TRSPRMHDALLSHGRRGVVSIANNTKAEQARSTEIGFNYNDGTFSFDGSYFWQNIKDA
                     LGTSTGRNNHLCPELTPNTNNKECYSEIINAGTIKNRGYELNAGYHNNGFTARVGVAH
                     SKPRFQGDRLSTNPEYASAIGRTWTASLSYRFDQPNLEVGVHHRIIEKVKAEDNYFVM
                     NNNGIPQFGRGGPTGKDGYNVTDITLNWKPLNDDSVNVNFAVDNVANKKYNAHGQRGQ
                     LSARGREFRAGVNYTF"
     gene            complement(23125..23412)
                     /locus_tag="PWX02_000232"
     CDS             complement(23125..23412)
                     /locus_tag="PWX02_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL1728292.1"
                     /translation="MAKTLRISSRAQNDIEEILASVIEYTGFESSGIRLQEDIYQKFE
                     TIAYMPSAAGRLREDGTREAFTRRYSIVYTELENEVWIITIIHSSRLYPRI"
     gene            complement(23405..23584)
                     /locus_tag="PWX02_000233"
     CDS             complement(23405..23584)
                     /locus_tag="PWX02_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728293.1"
                     /translation="MFKEKGYDEFLAEKIRLGLEDMQSGNGLSLDESKARTKQLIERK
                     ARELANFEQENIIYG"
     gene            23846..27745
                     /gene="purL"
                     /locus_tag="PWX02_000234"
                     /gene_synonym="purI"
     CDS             23846..27745
                     /gene="purL"
                     /locus_tag="PWX02_000234"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDL1728294.1"
                     /translation="MTIQTFRGSPALSAFRIQQLLQKFQQNQLPVKSVYAEFLHFVAL
                     NRPLVADEENKIKELLHYGPTLEEDEPTGFCLIVTPRIGTISSWSSKATDIAHNCGLA
                     AVERIERGTAYYLEFSETPNESQLTTLKGLLHDRMMETVLDNESQAEQLFAQQEPKPF
                     TSVDILGGGREALEEANVNLGLALAEDEIDYLVENFTALGRNPNDIELYMFAQANSEH
                     CRHKIFNADWTIDGVKQEKSLFKMIKNTFEKTPDYVLSAYKDNAAVMEGSTVGRFFPD
                     QDGQYRYHQEDAHILMKVETHNHPTAISPFPGAATGSGGEIRDEGATGRGAKPKAGLT
                     GFSVSNLVIPNFEQPWENPLSKPSRIASALDIMIEGPLGGAAFNNEFGRPALLGYFRT
                     YEEKVNSFAGEEVRGYHKPIMLAGGIGNIRAEHVQKGEIPVGAKLIVLGGPAMNIGLG
                     GGAASSMASGKSKENLDFASVQRENPEMERRCQEVIDRCWQMGAENPILFIHDVGAGG
                     LSNAMPELVHDGERGGKFDLRNILCDEKGMSPLEIWCNESQERYVLAVSPEKLDIFTA
                     LCERERAPFAVIGEATKEKHLTLADTHFNNKPIDLPMNVLLGKTPKMQRDASSKTVKN
                     PPLVTADIDPKEAFHRVLRLPAVAEKTFLITIGDRSVTGMVAQDQMVGPWQIPVADCA
                     VTTASLDSYHGEAMSIGERSPVALLDFAASARLAVAESITNIAATNIGDIKRIKLSAN
                     WMSAAGHEGEDAGLYEAVKAVGEELCPALGITIPVGKDSMSMRTTWEENGEQKSVTAP
                     LSLVISAFARVEDVRKTVTPQLRTDKGDTRLLLIDLGEGNNRLGATALAQVYKQIGDK
                     PADVVNVERLKGFFNAIQILVAQEKLLAYHDKSDGGLITTLAEMAFAGNCKIRAEIDR
                     LGTDDLGVLFNEELGAVIQVKTSDLESVQAVLKAENLANLTYDIGSVAQGDGFEITRN
                     GEMVFSQKRSELRGIWAELTHQMQRLRDNPECADQEFEAKKDPNNKGLSAFLTYDVNE
                     DIALPFINKGTKPTIAILREQGVNSHYEMAAAFDRAGFNAIDLHMSDLISGRRNLNDF
                     NAMVACGGFSYGDVLGAGGGWAKSILFNPMLRDQFSQFFVNPNTLALGVCNGCQMVSN
                     LAEIIPGTENWPRFVRNKSERFEARAAMVKINDTNSLWFNGIAGSHMPIAVSHGEGQV
                     EFKRSEQLENLQKQNLVIAQYVDSHLNPTEQYPANPNGSALGITAIGNTDGRVAIMMP
                     HPERVFRAVSNSWCPEDWTEDGAWMRLFRNARVALKY"
     gene            28105..28287
                     /locus_tag="PWX02_000235"
                     /pseudo
     CDS             28105..28287
                     /locus_tag="PWX02_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            28737..29114
                     /locus_tag="PWX02_000236"
     CDS             28737..29114
                     /locus_tag="PWX02_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDL1728295.1"
                     /translation="MDKSQGVVSVNIRGGSGFGRANNAIGPNYMGMLAGKYQFNDSTW
                     IGALFGHSWRKISQNYKIGGGRKVTDSSIDLSKIDDLDKESSTTSPFNAAHLKQKPMS
                     RMAKLEYGDKYQTITLLPKSGSG"
     gene            29844..30620
                     /locus_tag="PWX02_000237"
     CDS             29844..30620
                     /locus_tag="PWX02_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDL1728296.1"
                     /translation="MNYSATLSAYIHDLFTPFISYSQTHRAPNIKEIFFSNLGDYGVN
                     TALQPEQAKTTQFGINGFKERVFSEEDKLGFKVLFYNTHLKNYIYNVQHTAVGSAPSL
                     FIFHKNYEKPVNIKGIELEFSYDIGSFYGNLAYARQNTNQPVSFTDASSRVDAASHYG
                     FKTQGFGASKISALPRDYGSLELGTRWFDQRLQIGGVAKYYGKSKRIAIDEFNYTYYP
                     GTHIVKETHRLEETVNKQPIIVDFYVSYEPIKSLILKLEL"
     gene            31224..31718
                     /locus_tag="PWX02_000238"
     CDS             31224..31718
                     /locus_tag="PWX02_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1728297.1"
                     /translation="MSRKYKFHEKSGAYFVSFATVYWVGVFVREIYFQVIVDALDYCR
                     KHKGMILYAYCIMPTHIHLLFQAENQNPSDLIRDFKTFTAKILIQRIKDNLQENRREW
                     LLWMFSRAAERRCNVKQYQFWQHHNQPIEIYSQKFFDEKLNYIHQNPVVSGFVSEPWE
                     WKYN"
     gene            31973..32212
                     /locus_tag="PWX02_000239"
                     /pseudo
     CDS             31973..32212
                     /locus_tag="PWX02_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651213.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
     gene            complement(32411..>32527)
                     /locus_tag="PWX02_000240"
     CDS             complement(32411..>32527)
                     /locus_tag="PWX02_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HDL1728298.1"
                     /translation="IPKAHFELYLKECEWRFNHSDLKSQIFLLKQLVKGGLG"
BASE COUNT         9336 a         6663 c         6942 g         9586 t
ORIGIN      
        1 gctctaagag gcaaatgcaa ctcaaagaag ttttgtatct cttgtagtgt gagcagtggc
       61 tcaaaacaat aaatctttgt tttctgatcc ttaattttag gagttaaaaa tgaaaaaatt
      121 agtcaaactt tctttccttg caagccttgt gatgggaaca gcatctgttc aagcggctga
      181 aacatttgtg actatcggaa caggcggtca aaccggtgtt tattatgttg tgggtcaatc
      241 tatttgccaa ttagtaaacc gtgattcagc aaaaacaggt ttaaaatgta acgctccttc
      301 aacaggggca tcggttgcaa acttaaatgc gattgcagca aaagaaatgg atatggggat
      361 tgcacaatca gactggcaat accacgccta taacggctca agctcatttg aaggtaagaa
      421 aaacgataaa attcgtgcca ttttctcgct tcacccggaa ccgtttacag taatggctcg
      481 taccgattca ggcattaaat cgttcgatga tttaaaagca aaacgtgtaa acgtgggcga
      541 cccgggttcc ggtactcgtg caacaatgaa cgtgatttta gcggcaaaag gctggacgga
      601 taaagatttc aaagtggctt ctgaattgaa accggcagaa atggcatctg taatgtgtga
      661 taacaactta gatgccatca cttataacgt aggtcacccg aatggtgcgt taaaagaagc
      721 agccgcttct tgcgatgcga aattagtgcc ggtaaccggc cctgaaattg ataaattagt
      781 ggcagatcac tcatattatg ccaaagctac gattccgggc ggtttatata aaggttcgga
      841 tgagccgaca gaaaccttcg gtgtatatgc aacgttagtg acttctgccg atgtggatgc
      901 agataaagtg tacaatgtaa ccaaagcggt atttgataac ttcgaccgtt tcaaacgttt
      961 acatccggcg tttgaacact taaaacaaga agagatgatc aaaaacgccc tttctgctcc
     1021 gttacacgaa ggtgcaattc gttactataa agagaaaggt tggattaagt aatatttcct
     1081 ttctgtaatt tgattcaatc agcacgtttt ttagcgtgct ggttttttgc ttttttgaaa
     1141 ctgttgataa gttatcgatt ttcaccttta attgataact tatcaacagt gttttcacga
     1201 gcaagtggtt gttttttgag gaaatttgta aaatttaata agaaaatgac cgcttgcgtt
     1261 ttgaggagtc tttatgtcag ttgaatctaa ggttgattac gatgacctac aagatatggt
     1321 tgcctccaat gacagtgggg ggcgaacccc gtccggtctt gcgaaaaaag ccattgtgta
     1381 tgtcgccatt ctttggtctg tgttccaaat ttattatgct tctccattgc cgtttgtatt
     1441 ccaagaatgg cttactcacg caggcattaa tttgaatgtg gtggtggacg acaccaaagc
     1501 ccgttctatc cacttagcct ttgcaatgtt cttggcgttc ctatcttacc ctgcttttgc
     1561 cagctcgcct aaacatcata ttcctaaaat agattgggtg tttgccatcg taggagctgg
     1621 gttagcgttg tattatattt tcttctatga aggtttggtg aaacgttttg gagcgccaaa
     1681 tacgcaagat attatcgccg gctgtttagg gattttattg gtgctggaag cgacccgccg
     1741 tagtcttggc ttaccgcttg tgattattgc gattgtattc ctgatctata acttcttcgg
     1801 gcaatatttc ccggcagatt ggattgtaag ccaccgctca ggttcgcttt ctcaaattat
     1861 caaccaacaa tggattacca ctgaaggcgt gtttggtgta gcccttgggg tttcgaccaa
     1921 atatgtgttc ctgttcgtat tatttggggc gttgctcgat aaagcaggag caggtaacta
     1981 ctttattaaa accgcctttg cctatttagg gcatttgcgt ggtggtccgg caaaagcggc
     2041 ggtggtttct tccgctttaa ccggcttgat ttcaggctct tcgattgcaa acgtcgtgac
     2101 gacaggtaca ttcactattc caatgatgaa acgggtcggc tttaccaaag aaaaagccgg
     2161 tgcggtggaa gttgcctcat cagtaaacgg tcaaattatg ccaccggtaa tgggggctgc
     2221 agcgttcttg atgatcgaat atgtgaatat gccgtatagc cagctgatta ctcacgcctt
     2281 cttacctgcg ttaatttcgt atattgcgtt ggtgtatatc gtgcatttag aagcttgtaa
     2341 gatgaattta caaggcttac caagagccga agagccaagc ccgattttag tcttcttgct
     2401 aagaaccgta gcttcaattt tagtgatcgt aggattagcg attggcgttt actacggctt
     2461 aggttggatt cgtgatgtgg cagaagatta tgcgtttatg attgtctgtg ctttcttagg
     2521 cttatggtat ctactggcta ttcgccgtgt ggcaagctac cctgatttag aagcggacga
     2581 tccaaactca gaaattgttt ctctaccaaa aagaaaacca accgtaaatg caggtttaca
     2641 ttaccttatt cctgtggttg tgctactttg gtgcttaatg gtggaaatgc tttcgccggg
     2701 cttatccgca ttctggggaa ccattacctt aatttttatt caattaaccc aacgcccgtt
     2761 actgaatttc ttccgtaaag agagcgtaac caaagagatt atcaaacaag gtgttcgaga
     2821 tgtggtagat agccttgagg ctggcggtcg taatatgacc ggtatcgccc tcgctaccgc
     2881 aacagcaggg attattgtcg gcgttgtggc actaacgggc tttggggttc agctttcaag
     2941 tgtgattgaa ctcctatcaa tgggcaacat tgtgctaatg ttgatcttgg tagccgtatt
     3001 cagcttaatt ttgggaatgg gcttaccgac aactgcaaac tacattgtgg tgtcatcatt
     3061 aatggcaacg gttatcatgg aagtcggtcg tcaaaacggg ttaatcgtgc cgttgattgc
     3121 ggtgcattta ttcgtattct attttgggat tatggcagat gtgactccac ctgttggttt
     3181 agcctcgttc gctgcggcag caatttcagg cggtagcccg attaataccg gtatggtggc
     3241 atttaaatat agtttaagaa ccgtcatttt accgttctta tttattttta ataccgattt
     3301 attattaatt gatctgcgtt atccggggca cggctgggtt gtgtttatta ccgcaactgt
     3361 tggggttctc gcctttacct ctgctacaat gcaatatatt gtgactaaaa acaaatggtg
     3421 ggaaacggcg ttgctggttg tagccgcttt cgccttgttc cgtccgggct tctttatgga
     3481 cactgaacgt catatcgagc cggtgcagtt tgaagaacag ttgcttgcaa gcaaagtagg
     3541 tcaaaatgtt actttaaaag tagagggatt aaacccttac ggtaaagaaa ttgagttcta
     3601 cgctcaatta cctgtaccgg aaggagcaac cggcgaagag cgtttgaaaa acttaggctt
     3661 aacgctaatt caaaccgatg aaaaaatcaa agttgatggc gtagaaacac caaaagtggt
     3721 gattgatatg gtagaaatgg attctccggc agctaaagca ggcttaaact gggatcaaac
     3781 cattcactac attgccgtac cgcaagatgc tccttctaaa gattggatct tcattcctgc
     3841 tctattgttg gtatttggta ttgtggcaaa ccaacgccgt agagtgaaaa aacagtgtgt
     3901 tattgcatag tatttatatg ctatgtaggg acgggtcctg tgcccgtccg ctattttagt
     3961 taatattcgg gcaggcacag gacctgcccc tacggaataa atatgtataa caaattactt
     4021 attgcgattg atttagccga cctaaaaagt gctaaatatg tggtagatac ggcattacaa
     4081 atgacggcaa aaaatccgaa tgcggtttat cgggttgtgt ctattattga gccggtggat
     4141 aacagcttaa tttctgcatt cttgccgaaa aatttcgata aagaagtggt ggctgaagca
     4201 aaccaaaaat tgcacgaatt taccaaaaac cattttcctg aaggttcaaa agtgcagcac
     4261 attgtggcgt atggtacgat ttatgaagaa atttgccgta ttgccgatga aaagtcggtg
     4321 gatttaatta tgatgctggc aagcagcaaa ccaaatgcga agggcttaag ctctaatacg
     4381 gttaaagtgg ctcgcaacac caccaagcca attttagttt taagataatc ccgcattcag
     4441 ttacaatagt gccattctgt cggaatggca tttttattta tgaaaaaaca atcgttcaaa
     4501 gcactttctt ctagagcaat aagtgcaacc attattttac aggtgttaga tcaaggtaaa
     4561 tcactttcca ccttgattcc tgacgctcaa aaacagcttg aaccaaaaga tttaccctta
     4621 gtgcaagaaa tcaccttcgg agtgtgccgt gtgctgcctc gcttggaatc gatcatcaaa
     4681 ttattagttg aaaaaccgct gaaaggcaaa acccgtttgg tgcattgctt actgttagtg
     4741 ggtttatacc aactgcttta tatgcgagtg ccggcacacg ctgcggttga tgaggtagtg
     4801 aatgcaacta aaacgctaaa actggatagc ttccgagcct tgactaacgg tgtgctacgc
     4861 cgttttctgc gagagcaaga agaaatctta gcgaaggtag ataaacattg gcaaacgctc
     4921 caccctgaat ggttggtcaa taaacttaaa aaagcctatc cgaactggcg agaaattgtt
     4981 gaagcaaaca accaacgccc accaatgtgg attcgagtca atcaacaaca tatcaagacc
     5041 aatgattatg cggcgttgat tagcgagctg gttgagcctg aatttgcaaa aaattcagaa
     5101 aatccgaccg cttgcgtgcc aaagtgtgct ttattgctgg agagagcggt aaatgtgaac
     5161 caactgccac atttcgagca aggttgggca accgtgcaag atgctcacgc acaatggtca
     5221 gcggagttgc tcggggcaga aaatggtgaa accgttttag atgcctgtgc cgcaccgggt
     5281 ggcaaaacta cccatatttt agaaaaagca ccgaaagcaa aggttatggc gttggatatt
     5341 gaagagagcc gtttaagccg agtgcgtgaa aacttagtcc gactggggca gattgcacag
     5401 gtaatttgtg gcgatgcttc caaacctgag gaatggcttg aagagggtgt gctgtttgac
     5461 cgcattttac ttgatgcccc ttgttccgca accggagtca ttcgccgcca ccctgatatt
     5521 aaatggttgc gtaaagaggg tgatattgca gaattagcca cattacaggg tgagattctc
     5581 aaggcgttat ggcaaaggtt aaagccaaac ggcattttgt tatatgccac ttgctcggtg
     5641 ttaccgcaag aaaatagcga gcaaattcaa cggtttttac aagatacacc gaatgcaaaa
     5701 caacttgaaa tcgattttaa cggcgaaaaa gtgttagaaa aacagttctt cccacaacca
     5761 aacggtggtg atggtttctt ctacgcaaaa ctacaaaaag tggcagagta atgaaagtaa
     5821 ttattttagg ggcaggacaa gttggttcta cgctggcaga aaatttggtg agtgaagaca
     5881 atgatattgt cttggtggat aatgacccaa ctcgtttaga caagctcaaa gataaacacg
     5941 atttacaggt gattcggggc gaatatgctt cacctcaaac cttgcgtgat gccggtgcag
     6001 gtgatgctga tctattagtt gctgtaacta atagtgatga agtgaatatg atcgcttgcc
     6061 aaattgctta cactttattt aatgtgccga ccaaaattgc ccgtatttgc agtgctgatt
     6121 atgtgagaga acgagagcgg ttgtttaatg atgatgtatt gcctattgac catattattg
     6181 cccctgaaat tttagtcaaa gaagatattt tacgccttat tcactatccg ggggcgttgc
     6241 aaattactca ttttgctgat gagttagtca gtatcgtgaa tgtaaaagcc tattacggtg
     6301 ggcctttggt cggttacccg atttcagccc tgcgtgatca tttaccgcat attgatgctc
     6361 gggtcgtggc aattttccgc caagacaaag cgattgtgcc acaaggttca actattattg
     6421 aggcaggtga tgaagtcttt ttcatctgtg caacccaaca tattcgagca gtaatgagtg
     6481 aactacaacg gcttgatcgc ccacataagc ggattatgat tgtcggtgga gggagcatcg
     6541 gctcttcgtt agctcgggat ttagaagatc agtatcgggt aaaaattatc gaacgcaatc
     6601 cggttcgagc cgaaaaatta gcagaaaaac tatctaaaac gatggtgctt accggtgatg
     6661 cctccgatga agagttgctg tttgaggagc atattgagaa tattgattta ttccttgccg
     6721 tcaccagtga tgatgaagcg aatattatgt ccgccctgct ggcaaaacgg cttggggcga
     6781 aaaaagtgat tattttagtg caacgccctg cctatttaca cctgattcaa ggtggcacta
     6841 ttgatattgc catttcccca caacaagcta ctatttctgc tctcgctagt tacgttagaa
     6901 aaggcgatat tttacaagtt tcttcactca aacttggggt tgcaggagcg gtagaaatta
     6961 tcgctcacgg cgatgcaacc acttcaaaag tggttggacg acaaatccgt gaattaaaac
     7021 taccgcaagg ggcaattgtt ggggcggtgg tgcgaggcga agaagttatt atcgcccaca
     7081 aatccaccat gattgcagaa aatgaccgtg tgattatttt tgtgaacgat aagaaacaga
     7141 tcgatgaaat tgaaaaactg ttccaacttg gtgtattttt cttataaagc caagctccgc
     7201 attgctacat gaaacaatgt gctttttgtt tggtaaattt tcatgaaaat ttgaccgctt
     7261 gtaaaataag aaagccgcaa cgtgattgcg gcttttgtat aaatcgagct aattttaaat
     7321 taacggaacg gttgatctaa cggcacttcg gcatcaggtt cacgcgtaat acggttacgt
     7381 aaatcgcgac gaatcacctc aatggtccag aaccagaaaa tatgaccaac taattcagaa
     7441 atgtgttcat ataacggcca ttcagcaacc ggtggtgtta aacctaatgc cgggaaggtg
     7501 atgtagtgaa cacaaatatt agcgatgata ccggcaccga taccttgcca gaatttaatt
     7561 tttgggaaga tttccgccac aatgcagtaa ccaatcgcaa agactaacga gaaaatcata
     7621 tgggtgacac caatccagtt gaacgcgtgg tcagcaaagg taaatgctgc ttcagttgga
     7681 tcgataccaa tataatcacg taagaaaacg tgtggtgggt ttaaaaatgc acgtgaacat
     7741 tcttgtaatg cgatggtttt ggcagccact tggtctgctg cggcattcat tgcatctaac
     7801 acaggttgcg ggcaagcagc ggtaaacaga tcgatagggc tacgaggtgg gaaaggatgt
     7861 tctgcccccc attttacgaa tgctgaaata ataccggcaa taatcccgat aaatacagct
     7921 agcccataac gacggcggtt tggattggtt tgttggaaaa tactcataat gaaaatccta
     7981 atttgagtga taattgtgtt gtgttaatca agccatttta tggaatgtga tttcaaaaaa
     8041 ggctaatggc attctgcact caattaattt aaattgcaaa ttaattagcc aaaaagagtt
     8101 aaccataaat aattattaaa catttactta acattagaat atttttgttt gcaattttga
     8161 tacaaggtta acctaaaaga aaactcccgc ttattttgat aagtgggagt ttttagaaga
     8221 atctatactt tatcatcaaa ttgcagataa accacctgag tttgtaggta ttcctctaag
     8281 ccgtgcttac cgtcagcacc gccaataccc gatttacgcc aaccggcgtg gtagccttgc
     8341 attgcttcaa agttttcacg gtttacataa gtttctccga attttaagcg ggaaattact
     8401 ttcatcgctt tattgatatt ttgcgtgtaa accgaagagg ttaagccata ttcacaatca
     8461 ttggctaagc gaatcgcttc atcaatggta tcaaatgtcg caaccggaat gaccggacca
     8521 aaaatttcgt gacgcataat atccatagag ttatctacat tgtcgataat agttggctca
     8581 aagtagtagc ctgttccttc aaccacgtta ccacctaata ccagattacc tccttgatgc
     8641 agtgcgtgtg tcaccatttc ctgcacttta tgcaagcctt tttcatttac catcggcccc
     8701 atatcaatgt cttgttgttc taatggatta ccatatttca ccgcttgcat tttctctacc
     8761 aatttttgga caaattgctc tttaattgaa ctctgtacat aaatgcgttc tgcacagtta
     8821 caaacctgtc ctgaattgat gattcgagaa tttactacag cggtagcagc aagctcaaga
     8881 tctgcatcgt ctaatacgat agccggtgct tttccgccca attccaaatt gactttaatg
     8941 atattcttgc tggcggcttc catcactttt tgtccggcaa actggctacc ggtaaaggaa
     9001 accataccga ttttttcatt gcctgaaagt tccggcccga cttcattgcc atagccggtg
     9061 acgacattaa ataccccctt aggtaaaccg acttgatgca cgatttccga aaagagaatc
     9121 gcattattag gtgcatcttc actcggttta atcacaatag tattacctgt tactaatgcc
     9181 ggagccgctt tacgggcaat gaggaagaac gggaagttcc aaggtaaaat gccggtggtg
     9241 acgccaatcg cttttttgta taggaaaata tgttcatttg ggcgatcact ttggataatt
     9301 tccccttcat aacgtctcgc ccattctgcc atatagtcta agtagtcagc ggtaaaatgt
     9361 acctcaacgg ttgctaagga cagagtttta cccatttctt cggaaatagt gcgagcaatt
     9421 tcatcggcac gctcacgaat ccccacagca atttgacgta aatattttcc tcgttcaatc
     9481 gctggtaaac gttcccaagc ttcttgggcg gcttcagcag catcgatagc gattttggca
     9541 tcatcagctc cgccacgagg gacttcggag atagcttttt cggtggcagg gttatagact
     9601 gctaaagtgc gaccatctaa cgcaggcaca aattcaccat taatatacat tttatgttgt
     9661 ttcatcatga gttgctcctt ttttatccac agtggaagtt gatgactatc attcggtaac
     9721 tgaaccgcct ttgcaaacaa attgttttat ttttgttaat ttatagatat aaatgtgaac
     9781 aaaatcacat ttcttggcga taaaaaagca acgaaaatgc ccgatttttc cataaatatg
     9841 agaaatcggg cattatgact atcagtattt ttttaacaga aattgcaaat ttttattaaa
     9901 aaatcaccgc ttgtctattt aacaaagata gaacttagta tgcgatccga tatacctcca
     9961 acagctcctc agccgtacaa tctctcgggt tgccaccggt gcagacatca ttaaaggcat
    10021 cgaccgataa tgccgctaaa tcggcttctt taacgccgat ttgatgcagt tttggcggaa
    10081 tgccgacatc ttgtgaaagc tgttgtgcgg cagcaatggc ggcgttgcgg tactcttctt
    10141 ggctcatttc atctacgcct tttaccccca ttgctcgggc aatttcacgg tatttttcgc
    10201 cggtgtaatt tttattaaat tccatcacat acggcagtaa tactgcgttg gcaatgccgt
    10261 gtggggtgtc gtaataggca gaaagcgggt gtgccatact gtgaaccgcg ccaagcccaa
    10321 cgttggagaa gcccattcca gccacatatt gtcccaatgc cattgcctcg acatctttcg
    10381 gatttttctc cactgcacca cgcaatgaac ggccgatcag ctcaatcgct ttaaggtgta
    10441 gagcatcggt taattcccac gccgctttgg taatgtagcc ttcaatggcg tgggtgagag
    10501 cgtccatacc ggttgcggca gccaagcttt tcggcataga ttccatcata tctggatcga
    10561 ctaacgccac tgccggcaca tcgtgaacgt ccacgcacac aaatttgcgt tttttcgctt
    10621 catcggtaat gacatagtta atcgtgactt ctgcggctgt acctgcggta gttggaatgg
    10681 cgataatcgg cacacatttt tggtgggttg gggcgacacc ttccaatgaa agtacatcgc
    10741 taaactcagg gttattgatg ataataccaa tcgctttggc actatcaatc ggtgagccgc
    10801 caccaatcgc aattaaataa tcggcactgg aggctttgaa ttttgcgacc cccgctttga
    10861 tcacatcgac tgccgggttg gctttgacct catcgaaaat ctcataaggc aagcctgcgg
    10921 catcaagcaa ggcggtgact ttttgggcaa cgttaaattt gatcaggtct ttgtctgtta
    10981 ccacaagggc ttttttgaaa cttcggctgg tcacttcgtg aacaatattt tggattgctc
    11041 ctttgccgtg gtaactggtt tcattgagga taagacgatt tgccatacta ttttccttct
    11101 taaaagttaa acaattcgat taacttgcaa gcggtcaaat tacgtcattt ttttgcaaat
    11161 tttgataaaa atttcaccgc ttgttgcgat tcttttgttg tgagtgtgct taggctaaat
    11221 attcaaaata cgtttgattt catcacggta ggcttcggca tcgctaccaa aaatagattg
    11281 aataactgag cctgaggtca caacgcctaa cgcaccgaca gatttaattt tatcccattc
    11341 cacaatgtcg gtatttttca cctccacacg taggcgggta atacaggcgt aaacatcgac
    11401 aatattttct cgaccgccaa gtgcggcaat caccactaag gctttatcgg taagggtatt
    11461 tgacatattg gcttcctttc ggctgaaagg gtgggcaacc ccaccccgtt taacttgaat
    11521 gttagtaaag aacacctttc gcttcttcgc tatcaatgtt ttctttcgtg acataaacca
    11581 ccccggtatc aatcactttt tcgactttct cgcctttaat cagcttgccg atggtgtcca
    11641 cgcctttata gcccatttgg taggaagatt gcaccacgat gccgtctagt gttccgtcac
    11701 gcacgaagtt ttgtaggtcg gagtttccat caaagcccac cgctacaatt ttgccggcaa
    11761 agcctttttg tttcaccgct cgtgccattc ctaccgcagt cggttcgttc gccccgaaga
    11821 tcgctgcaag atcagggtta gaacctaata cgtcttcggt ttggtttaac gctttggtca
    11881 tatccgaatc ggagtagtac gggccgacca gtttgtaatc ggcttttgct ttgatttcgt
    11941 caataaaacc accggtacgc tcaatcgctg atgctgcccc ttgggtaaag ctcatcactg
    12001 ccactttgcc gcctttgact ttggcaagca gtttttccgc tgctaattta cctgctgcac
    12061 ggttgtcggt ggcaaggaaa gattggtagt atttgccgtc actgttgatg ccggaatcga
    12121 taagcaccac cggaatgcca ctctcataag cttttttcac cgccggcact aatgctaccg
    12181 ggtctgaggc agccagtacg atgcctgcca cgccacggtt tacggcgttg tccaccatat
    12241 tcacttgggc gtcaatcgct gtttcggctt ccggcccttg gaaggtgact ttgtattgtc
    12301 ctccaatatc tttgccggca gtttcagccc ctttacgcac gttttgccag aaggtagagt
    12361 tggcagattt cacaatcaca gcgatttcat cagattttgc cattgcactg cctgcaaata
    12421 aggtcgataa accaaagagg gtcgcagtaa ataatgctga ttttttcatt ttaattctcc
    12481 tttaagagtg gattattttt taccggcttt tttggattgg cggagctggt cgagagagac
    12541 cgccacgata atcacaagcc cgatgacaat catttgaaca aatgaagaaa cgccattcat
    12601 attcaaacca ttacggagta ccccgataat gaatgacccg atgagtgtgc cggggatagt
    12661 acccacaccg cccataagcg atgtgccgcc caccacagca ctggcaatcg catccagctc
    12721 gtaagagacc ccaccgtttg gctgggcggt gacgaggcgg gaggcaagga taacgccagt
    12781 taaaccagag agtaagccac tggcaacata ggcatagatt ttgacaatat tggttttaat
    12841 gccggataag cgtgcggctt cttcgttaga accaattgcg taaagatagc gtccgacttt
    12901 cagtttagag agagcgaaag tgaatagtac ggtaataaag atcataataa tcacagggta
    12961 aggaatacct gcaaatacca ctttcggcaa gccgtttgcc ccttcttcca caattttaaa
    13021 taatgctccg ttaccgagat tggcaaagga ttcaggcatt ccggaaactg gtgcggcatt
    13081 ggtaacatac aatgctaaac cacgggcaac catcatcata ccgagggtag caataaacgg
    13141 aggcaatttc attctggtga ctaaaatgcc gttgattaag ccacaaatac cgccgaccaa
    13201 aatccctaaa atcattccta gtggtacggg tacaccggca tttacaatca ttgcagctgc
    13261 cacaccggag agtgcaacta ctgaaccggt acttaaatca attccaccgg ttaagatcac
    13321 acaggttgcc ccaataccaa ttaacgcaat ggtagaggtt tgtagcccta ctgtcataat
    13381 gttattggag gtaaagaagt attcattggt tacgctgaaa aagatgatta atagtactaa
    13441 gccagccaat gcagccattt ttcttaacat ttctttttgt ttttcattca tttttccacc
    13501 ccatattcac gagcaaacat atttttaaca ccagttgcat aatgcataat ttcttcttgg
    13561 ttggtttttt tagtctcgag catacctgtc attctacctt cacgcattac aatgatgcgg
    13621 tcggaaactc ccaagacttc aggcaattcg gaagagatca cgataacacc cacgccattg
    13681 gcagcaagct catcgagcag ttgatagata gcatattttg ctccgacatc aattccccga
    13741 gtcggctcat caaaaatcat cacttttgct tcacggaata accatttacc aattaccact
    13801 ttttgttggt taccaccact taagttttga actttctgct caatcgttgg ggttttgatt
    13861 tccattttgt cgatgaaagt ttttgaggct ttttcttctt catcgtaaga aatcacacct
    13921 atttgattgg caactttatc catagatgcc atcgtgatat tctcacgaat cgacattctt
    13981 actgcaacac cattgagctt acggtcttcc gacaaatagg ctataccggc tttaattgca
    14041 tcactcggat ctttaatgct gactttgttc tcgtgcacaa aaatctcacc gctatcaagt
    14101 gggtctgctc cgaagatggc tcgagccagt tcagtacgtt ttgctccaac caagccggta
    14161 atgcccagaa tttctccttt gcgtaagtca aagttcaatg gttcaaacac tcccttacgt
    14221 tcagcattca taatcgagag gataatgtcg tttgtcacca cagcggtttt tggtgggaaa
    14281 tggttatcca atgaacgccc aaccattttg gtaatgatct catccatcgt gatttcattg
    14341 aatttatggt tagatacata ggtgccatca cggaaaatcg taatatggtt acagatacgt
    14401 tttaattctt ccattcggtg agaaatatag acaatgctca cccctttggc acgcagtcta
    14461 tcgaccaatt caaataattt atcgatctct ttttcggtaa gagcggaggt cggctcgtcc
    14521 ataatcagta cttccacatc aaatgaaagg gctttggcaa tttctaccat ctgttgtttg
    14581 gctaccgaca acattctcac ttcatcgttt ggatcaacat caatctcaaa gaaattcagc
    14641 aatttagccg attcttccac cattttgtct ttatccacta aaccgtgttt gagtggctca
    14701 cgggcgaaga aaatattctc tgccaccgtt aaatggggaa tgaggttcag ttcttggaaa
    14761 atcatactaa tgcccgcttt ttgtgcctct aataccgact caaagcgaac aggctgacct
    14821 ttatatatga tttcaccttc atcggcttga tatacgccca gcaagcattt catcaaggtg
    14881 gatttacctg caccgttttc gcccattaat gcgtgaactt cgcccttacg cagcgtaagc
    14941 tcaacacctt ttaacgcatt gacccccgca aaggatttac tgatgttttt catcgtgagt
    15001 attacttcat tttgcatact gcactccgtt tgtaaaaatt ttaagaaata tcaccgcttg
    15061 tatgttattt atggcaggct attgttcgct ttccacccaa gaaccatagg acttaaattt
    15121 tccgagtacg atatccattt gttctttgtt tagcgttggg ataggttttc ctgtagcgag
    15181 cagtttcaaa taccattctg cgatcacttc cacctcgtgg gcgatgccga gggctttttc
    15241 gagggtttta tctgcggtga ttaagccgtg gtgagcgagt aaaattgctt tgctgtgttt
    15301 aatgccttca cgtacatagt cggctaattg gtgagtgccg aacggggcat aaggtacaca
    15361 aggaatgtga ttagtgccgg tgcaggcgat catatagtga atggcaggga taggttcacc
    15421 taaaatagac actgcggcac agtttttagc gtggttgtga actaccgcat ttaattcagg
    15481 gcgagcctca taaaccgcta aatggaactg ccactcactt gaagggagtt ttccctcttc
    15541 gtgtttgccg ttgccatcaa caaagacgat agaatcttcg gtcatttgtt cataaggtgt
    15601 acctgttggc gtaatgagca taccgtcttt ataacgtacg ctcacattgc ctgctgtacc
    15661 ttgatttaac ccaattcggg tcatttcaag acaagtatca atgatttgtc tcgccagtat
    15721 ttttctgtcc attgttgaac tccttttaat tttatatttg taaaaaattc tgctttttta
    15781 accgcttgta attaaaccgg tgttacgcct tttttgataa taatgttgcc gtatttggct
    15841 aactcgcctg taatgacaac ggcataagcg tttttggctc tatcatagaa agcgaagcgt
    15901 tctacacgct ctattttcgg ggctgttccg tttacttttt taattgcatt gaggtaacgc
    15961 tcttcaacgc ttggatcgag cgtgtcgcct tttactgctt gcatcattgc aagtggggct
    16021 tcaacgtatt ggtcaaattc aaacagagca gaaatgcctt ctaataaggt ggcaacactt
    16081 aagccatctg cacgaatgac gttttgatga atagagtgag cagggaagtg tgcatcagaa
    16141 agcactaatt catcgccgtg ccccatttcg gctaagactt ttaataaatc tggagaaatt
    16201 gccgggtgaa taccttttaa cattttgaac tcctttttta ccctctctct gatttgaggt
    16261 tctgaatgaa cctcaaatca gagagagggg agaaacttta tttataaaat tgactatttg
    16321 acgatggctc aacccgtttc acatttggct tcattgcctg ttgagccgca tttggatttt
    16381 caaatacacc agcacctgta aaagtaaaca ttgccgcgcc taatacggta ctttcaggga
    16441 aatccactac atcaatcggt ctatttaaga catcggctcg aatttgattc cataaagcgt
    16501 ttttagaacc acctcccaca caaatcaaac tctcagcatt aaaatgaccg acttcttgta
    16561 atacatctaa cccttctttt agacgatgag ccatatattc caagcctgca cggtaaattt
    16621 cgccacgagt gatgtgcata gataagccga taattgaacc tgttttagct gttgtgccat
    16681 caaaattgcc gactaatttc acaccgtttg agccagcagg cacttctgcc gcttcttcaa
    16741 tcattgtgcg gtagtaatgt tcagtattgg ctatatcaga gaagaaacgt ttgccaaacc
    16801 actccatcac acctgaagct acccactgca cacctgggtt gaaataacca gattggctat
    16861 caaactcaat ggttaagccg ttttgaacga attgccattg tggttttgct tttggggtac
    16921 gaaccattaa aatttcccaa gtgcctgaac ttaacacagg ttggttatag ccagctcctg
    16981 agccaaaaat ggcaaattga gtatcgtgtc cgcaagaaat tactggaatt tgaccgctta
    17041 aacctaactc ttgggaaatt tctcctttta atgttcccac aacttctcct gcacttttca
    17101 ttggaggaaa atggttttcg gttaagccta ataaatctaa aactgtttta tcccacgaat
    17161 cgttttcgat gtttgtcatc attgacgttc ccgccattgt gcggtcggtt gtttgcacgc
    17221 ctgtaagacg gtaagtcaaa atagatgaga tgaataagaa actctcagct tgttgataaa
    17281 tttctggttt gttctctttt aaccacaata atttgaataa ggtgttgaaa ctataatgtc
    17341 caacgccatt acgttgatat aacgcctcaa catcaagata attggcaagg ttttccataa
    17401 tcggtagggt tctcgggcat ttccaagaaa tgatcggata aagctgcttg ccgtttttgt
    17461 caaataatgc accgtccacg ccaaaggtgg ttactgaaat cccaacaatc tcttgtcgtt
    17521 gttgttctga aagttgagaa agcgtctgtt tggcacaggt cattaatttc gaccagatct
    17581 cttcaatatc ccaaatatga aattctgggt tttccacatc tggttgtgtg ttattcggta
    17641 gatgatgggc tgccacaatt tcacccgctt gattaatcgc aatcgtacgg aggttggtgg
    17701 caccacagtc gaaaataaga gaaattggca ttgttatctc ctttacttat cctaatatct
    17761 cccttttaga gagatagatt aaattgcgtt tagcaattta atcagagaga gggatttgta
    17821 aattttgaga gaaaagcgac cgcttgtagc cctctccctg ccattcaatt ctgaacgaat
    17881 tgcttggctg tccctctccc acaaggggag agggtgattg tgtattattt atataatggt
    17941 ccgaagttag cacaagcacg gtagtcttga ccttctttgt cttgaccgaa accgttccac
    18001 gcacttgggc ggaatacgtc tttatcttcc acattgtgca tacaaaccgg aatgcggagc
    18061 attgaggcga gggtaattaa gtctgcccca acgtgtccga aggttgccac acagtggttt
    18121 gcaccccagt tcgccattac gctatacaca tcggtaaatg cgccttttcc ggttaagcga
    18181 ggtacgaacc aagtggtcgg ccaggtttca ttggtgcgtt tgtttaaaat gtcgtgtaca
    18241 ttttccggca agtcgataga ccaaccttcg gcaatttgta aaaccggccc gatacctttg
    18301 atcaggttga tacggtgcat cgtaaacggc attccacctt tggttaagaa ttgggaagat
    18361 aaaccaccac cacggaaata ttcgtgaact gccggacacc aacaggtgtt ttctaaacaa
    18421 cgtttaccgt cttcttcagt cacttcccaa gccggtttaa tagttggttt gcctgctcta
    18481 tcggtatgct gacctgttcc gtctaacgcc gcagagccgg agttgattaa gtggataaaa
    18541 ccgctttccg gacgccagcc ggtgacacgt tctacctggt cttcgctcca gtatgtccgc
    18601 acatcggcaa agatttgtgc ctgaccggtt aataagttac cgaacaacat attcacgcca
    18661 tttaagctgt cgttttcagt tgccataatg tatggtgcgc gaacgccgtt ccagtcatag
    18721 gtcgaattca aggtagcttc cataaaatcg ccgtttggca aatggtctgt ccattggcgt
    18781 tgcccttgga aaccggcagc aatcgcatta tgccctaagg cttcttcact atagcctagc
    18841 tctgctaagc gaggattacc caccattaaa tcacgggcaa taatggtcat tttcaccacg
    18901 tcttcccaga gtttttcacg ctcttccggg ctgcgttgat tttccggtgc gttgacatca
    18961 ataccttctt tgcaataggt tttcacccag ctcattgcta atttaaattc ttcggcatcg
    19021 taaatatggc gatctaaacg gcgtttaatt tcggtcatat ccacatattc gttacgcata
    19081 cccaaatatt cttggaagaa ttgttggttt acgatagaac cggcaatacc cattgaaaca
    19141 gagccgatag ataaatatga ttttcctcgt aaggttgcca ctgctaagcc ggcacgggca
    19201 aagcggagta atttttcacg cacatcgtct ggaatagatt gatcgtcagc ttcttgtact
    19261 tctgtgccat aaatggagaa tgccggtaaa cctaattggc tatgacctgc taaagcagca
    19321 gctaaataaa ctgcacctgg gcgttctgtt ccgttaaagc cccaaatggc tttcggcata
    19381 tgtgggtcca tatcaatggt ttcagaaccg tagcaccagc aaggggttac ggtaatcacc
    19441 gctccgacat tttcacgttt gaatttttct gcacaagccg cagcttcggc aacaccaccg
    19501 atggtggaat cagcaatcac acattcaacg aaatcaccat ttgggtggcg gacttcagat
    19561 tgaattaatt gtgcaacggc ttttgccata ttcattgttt gatcttcaag tgattcacgc
    19621 actcctaaac ggcgaccgtc aatggttggg cggataccaa tttttacagg ggtagatttc
    19681 aacgttgtca taaatagttc tcccgattgg ttaaaaaaat ttgcgattga atttattatg
    19741 catacgcaat atgagttatg aagaaaaatg cgaatttagt ttaaattttg tgagtgcgat
    19801 cacaaaaaat gggggatttt ggtaaaaatg agtgaaaaaa gaatgcccga tttgttttat
    19861 ttctgaataa aatcgggcag aaatcactaa ataatgattt caatttgatg tgctaagcaa
    19921 taatttttga gaaagtccgg tgggggttgg ttgataaata atttatcaat gcaagataaa
    19981 tcttcgatac gaaacaagtc tttacgctca aatttactgt gatcggctaa taaaaaaacc
    20041 tgtttactgt tcgcaattaa tttacgttta agggaaatat taatttcatt tgattcccaa
    20101 atcgccccat cggcaatgcc tgtacaagaa agaatggcaa gatcaatttt taaacgggtc
    20161 agtaactgct cggataatgg accatagaag gcatcgtatt tttcggaata aataccaccg
    20221 gtggcaatcg ttttaatgtg cggtttgtgt gaaaggctgt agataatccg cattgaactg
    20281 ctgacaacgg tgcaaggaat atttggaata cgctgtgcca cattccaaga ggttgagctg
    20341 gcatctaaag caatcaccga ttctggttgt aaatattcca acacattttc tgcgatactg
    20401 cgtttcgcct cactgttcag ccgttgtcgg gtggtaaatg agcgaccaat atctcgcttt
    20461 ttatggctca cagctccacc gtgagtgcga tgtaataatc catctttatc tagcttattt
    20521 aaatctcggc ggatagtttc aatcgaaacc ccacaatgtt ccgccaattc ttgaacgtta
    20581 gcttcatcaa attgggcgag ataagcgatg attttatctt gtcgttgctg cataaatagt
    20641 ccttagtaaa tttagacata aatttaactg cttgtaggtc ggtctgtcga gtggataaat
    20701 cggattttta gagcgtgatc acatttgttt tacaagcggt catatttaga gagtttttta
    20761 caaaatctac cttattttgt gaaatttaat ttcagactaa ttgacaacca attttaactc
    20821 tgtaaaattc gccttgtttt gatgttgaga attgttttta atatcaaaat tttggtaatc
    20881 ctcttaaata atttacctaa ggaaataaac agatgaaaaa cttcaaatta cttccggttt
    20941 ctgctgcggt attaagcgtg cttgcagcga acgcatttgc tgaaacagta gcaacggatg
    21001 cagcggtttt agatacggtt acagtaactg acaaccaagg tttaaaagta caaaccaatg
    21061 tagttacaac tcagaaaaaa gaagaaagta ctcaaacaga cttacgtggt ttattaaaag
    21121 atgagccggc aattagctta ggtggtggta acggtacatc acaatatctc tatattcgtg
    21181 gtatgggtca aaactcaatt gatgtaaaag tagataacac ttactcagat agccaaattt
    21241 tgtatcacca aggtcgccat atgttagatc ctgcacttgt aggtacggtt acagtacaaa
    21301 aaggtgcggg tagtgcttca gccggtattg gtcaaactaa tggtgcagta attgctaaaa
    21361 ccgttgatgc attagattta ttaaaaaaca gcgataaaaa cttcggtgca aaattaggta
    21421 ctggcttcag cacaaaccac gctcacaatt tcaatgcaac cttatatggt aaaggcgaaa
    21481 tctttgatgc tttagtctca ggtaatcttg tgagagatcg cgactataaa ggcggtaaag
    21541 attacgtaaa ccaattcggc acaaaccgtg taccatacag tgcgttagat aaaagtagct
    21601 tcttagcgaa attaggtgca actcttggtg atcatcgctt tgtattaagt cattctaatg
    21661 agcgaaatga aggggagcgt ttggttcgtg aagagtttga tgcagcacct gtaggtacta
    21721 aaggtagccg tgtaacttta gatcgtcaag caccggcaaa tcgtagaatg acagtacaaa
    21781 gaactaactt agagtggact ggtaaaaact taggttttgc tcaagaagca acagctaacg
    21841 tgtatcagtt agtacaaggt cgttggtctg agaatgaaac acgtaatggt tatgctggtg
    21901 gtgtagatta tgcaaccaaa actaaaatag taacccacgg tgcaaacgta aactttgatt
    21961 ctgcccttca cgaaaatgta ttattaaaat acggtgtgaa ctatcgccac caagagacta
    22021 aaccatatgc taaatttagt catcaattag tgaaccaaga gaaaaccgac accggtgtat
    22081 atcttgaagc gattactgct ccggttgata aagtgacttt aaccactggt gtacgttacg
    22141 accacttcaa attcaaagca atggacggta aaaaacgtag tgagggtgca tttaacccaa
    22201 gtgtaggcat tatttatgag ccaattcagc atttaagttt tagtgcaagc cataactacg
    22261 caactcgtag ccctcgtatg cacgatgcgt tactatctca cggacgtcgt ggtgttgtat
    22321 caattgctaa caatactaaa gcagaacaag ctcgtagtac ggagattggt tttaactata
    22381 atgacggcac cttcagcttt gacggtagct atttctggca aaacatcaaa gatgctttag
    22441 gtacatcaac cggtcgtaac aaccatctat gtcctgaact gacacctaat actaataata
    22501 aagagtgcta ctcagaaatt atcaatgcag gtactattaa aaaccgtggt tacgagttaa
    22561 acgcaggcta ccacaacaac ggttttactg ctcgtgttgg tgttgcacat agcaagcctc
    22621 gtttccaagg tgatagatta agtactaacc ctgagtatgc atctgcaatc ggtcgtactt
    22681 ggacagcatc tttaagctat cgctttgacc aaccaaactt agaagttgga gtacatcacc
    22741 gcattattga aaaagttaaa gctgaagata actactttgt tatgaataat aatggtattc
    22801 ctcaatttgg acgtggtggt ccaacaggta aagacggtta taacgtaact gatattacct
    22861 taaactggaa accgttgaat gatgatagcg tgaatgtaaa ctttgctgta gataacgtag
    22921 caaataaaaa atacaacgca cacggtcagc gtggtcaatt atcagctcgt ggacgtgaat
    22981 tccgtgcagg tgtgaactac acattctaat ctaatcccat ttagtcaaaa cgcccgaatt
    23041 atcgggcgtt ttttatggct tacaagcggt tatatttcag tgatttttgc aaataaaaaa
    23101 ccgactattt tagtcgtttg ttatttaaat gcgtggataa agtctgctac tatgaataat
    23161 ggtaataatc cacacttcat tttctaattc ggtataaaca atactataac gcctagtgaa
    23221 tgcttctctt gtgccatctt ctcttaaacg tccagccgca gagggcatat aagcgatagt
    23281 ctcaaatttt tggtaaatat cttcttgtaa gcgaattcca ctactttcaa aaccagtata
    23341 ttcaattacg ctcgctaaaa tttcttcaat atcattttga gctctactgc taattcgtaa
    23401 tgttttagcc ataaatgata ttctcttgtt caaagtttgc gagttctctt gctttacgtt
    23461 caatcagttg tttggttcgt gcttttgatt catcgagtga aagaccatta cctgattgca
    23521 tatcttctaa gccaaggcga attttttctg ctaggaattc atcatagcct ttctctttaa
    23581 acataatctg ctcctattaa gatataatta aaagcattgt ctgcttttaa ttttgagttg
    23641 tcaaattaac ttatttttaa attatctttt aaaacagatt gatagagggg accttctttg
    23701 aaaggtattc attagtattg aacaaattta tttgatacta atgaataaat gtatctaaca
    23761 gcaaacgtct gacaaatata gcaatcgttt gcgcaatcgt ttataatcgg tttagttttt
    23821 catcttaaat aggaaaatcc aaagaatgac aatccaaacc ttccgtggct cgcctgcctt
    23881 atctgctttt cgcattcaac aacttctgca aaaattccaa caaaatcaat tacctgtaaa
    23941 atcagtttat gcggagtttt tgcattttgt ggcattaaat cgaccgcttg tagccgatga
    24001 agaaaacaaa atcaaagagt tattgcacta cggtccaacg ctggaagaag acgagccgac
    24061 agggttttgc ctgattgtta cccctcgtat cggcacgatt tcctcgtggt cgtctaaggc
    24121 aaccgacatc gcccataact gcggtttggc agcagtagag cgaattgagc gtggcacggc
    24181 atattattta gaattttctg aaacgccgaa cgaaagtcaa cttacgacgt tgaaaggctt
    24241 attacacgac cgtatgatgg aaacggtgct ggataacgaa agccaagccg aacagctttt
    24301 cgctcagcaa gagccgaagc cgtttacttc ggtggatatt ttaggcggtg gtcgtgaggc
    24361 tcttgaagag gcgaacgtga atttaggttt ggcattggcg gaagatgaaa ttgattattt
    24421 ggtagaaaat ttcaccgcac ttggacgcaa cccgaacgat attgaactgt atatgttcgc
    24481 ccaagccaac tccgagcact gtcgccacaa aatttttaac gcagactgga cgattgacgg
    24541 cgtgaaacag gaaaaatccc tgttcaaaat gatcaaaaac accttcgaga aaaccccaga
    24601 ctatgtgctt tccgcttata aagacaatgc cgccgtgatg gaaggttcaa cggtcggccg
    24661 tttctttcca gatcaagacg ggcaatatcg ttaccatcaa gaagatgcgc atattttaat
    24721 gaaagtggaa acccataatc atccgactgc tatctcgcca ttccctggtg cagcgacagg
    24781 ttcgggcggt gaaattcgtg atgaaggggc aactgggcgt ggagcgaagc cgaaagcagg
    24841 tttgacaggc ttctcggtct caaatttggt tattccaaac tttgaacagc cgtgggaaaa
    24901 cccgctctct aaaccgagcc gtattgcttc tgcgttagat attatgatcg aaggcccact
    24961 tggcggtgct gcatttaata atgagttcgg tcgcccagca ttgcttggct acttccgcac
    25021 ttacgaagaa aaagtaaata gttttgcagg tgaggaagtg cggggttacc ataaaccaat
    25081 tatgcttgct ggcggtattg gcaacattcg tgctgaacac gtgcaaaaag gcgaaatccc
    25141 agtgggagca aaattaattg tgcttggcgg ccctgcgatg aatattggct tgggtggcgg
    25201 tgcggcttcg tctatggctt cgggcaaatc gaaagagaat ttggattttg cctcggttca
    25261 gcgtgaaaac ccagaaatgg aacgtcgctg ccaagaagtg atcgaccgtt gctggcagat
    25321 gggggcggag aacccaattt tatttattca cgacgtgggg gctggcggtt tatcaaatgc
    25381 gatgcctgaa ttggttcacg acggcgaacg tggcggtaag tttgatttgc gtaacatctt
    25441 gtgcgatgaa aaaggaatgt cgccacttga aatttggtgt aacgaatcgc aagagcgtta
    25501 tgtgcttgcc gtttcccctg aaaaactcga cattttcaca gcattgtgcg aaagggaaag
    25561 agccccattt gcggtgattg gcgaggcaac gaaagagaag catttaaccc ttgctgacac
    25621 acatttcaac aataagccga tcgatttgcc gatgaatgta ctgcttggta aaaccccgaa
    25681 aatgcaacga gatgcttcgt caaaaaccgt aaaaaatcca ccgcttgtca ccgctgatat
    25741 tgatcccaaa gaggcatttc atcgtgtatt acgtttgcct gcggtggcgg aaaaaacctt
    25801 cctgattact atcggagacc gttcggtaac cggtatggtt gcacaagatc agatggtcgg
    25861 tccgtggcaa attccagtgg cggattgtgc tgtcaccacc gcaagtttag acagctatca
    25921 cggtgaagca atgtcaattg gcgagcgatc acctgttgct ttgcttgact ttgctgcctc
    25981 agctcgcctc gccgtggcag aaagcattac taacattgct gcaaccaaca ttggtgatat
    26041 taaacggatt aaactttcag caaactggat gtcagcagca ggtcacgagg gggaagatgc
    26101 agggctttat gaagccgtaa aagccgtggg cgaagagctt tgccctgcac ttggcattac
    26161 aatcccagtg ggtaaagact caatgtcgat gcgaaccact tgggaagaga acggcgaaca
    26221 aaaatcggta actgctcctc tttctctcgt gatttccgca ttcgcccgtg tggaagatgt
    26281 gcgtaaaacc gttaccccac aacttcgtac cgacaaaggc gacactcgct tgctgttaat
    26341 cgacttaggc gaaggtaaca accgcttagg tgcgaccgct ctggctcaag tgtataaaca
    26401 gatcggcgac aaaccagctg atgtggtcaa tgttgaacgc ttaaaaggct tcttcaatgc
    26461 aatacaaatt cttgtcgcac aagaaaaact actcgcctac cacgataaat cagatggcgg
    26521 tttaattacc actcttgcgg aaatggcctt tgccggaaat tgcaaaattc gtgctgaaat
    26581 cgaccgcttg ggaacagacg acttaggcgt gctgttcaac gaagaacttg gggcagttat
    26641 tcaagttaaa acctctgatt tagagagcgt acaagcggta ttaaaagcgg aaaatcttgc
    26701 aaatcttacc tacgatatcg gttcagtcgc acagggtgat ggctttgaaa ttacccgaaa
    26761 tggcgaaatg gtattcagcc aaaaacgctc cgaactgcga ggcatttggg cggaattgac
    26821 ccaccaaatg cagcgtttgc gtgataatcc tgaatgtgca gaccaagagt ttgaagcgaa
    26881 aaaagacccg aataacaaag gcttatcggc gtttttaacc tatgatgtga atgaagacat
    26941 tgccctgcct ttcatcaaca aaggcacgaa accaaccatc gctatcttgc gtgagcaagg
    27001 cgtaaacagc cactacgaaa tggcggcagc gttcgaccgt gctggtttta atgcaattga
    27061 cttgcatatg tccgacttga tctctggtag acgtaaccta aacgacttca acgcaatggt
    27121 ggcgtgcggt ggtttttctt acggcgacgt actgggtgcg ggcggcggct gggcgaaatc
    27181 aatcctattc aacccaatgt tgcgtgatca attcagccaa ttcttcgtta acccgaacac
    27241 gctggcatta ggggtatgta atggttgcca aatggtctcc aatctagcgg aaattattcc
    27301 aggcacagaa aactggccac gcttcgtgcg taataaatcc gaacgttttg aagctcgtgc
    27361 cgcaatggtg aaaatcaacg acaccaactc gctctggttc aatggaatag ctggcagcca
    27421 tatgccgatt gcagtcagcc acggtgaagg acaggtggaa ttcaagcggt cggaacagct
    27481 cgaaaacttg caaaaacaga acttggtcat tgcccaatat gtcgatagcc atctcaaccc
    27541 aaccgaacaa tacccagcca acccgaacgg atcggcgttg ggtatcaccg ctatcggtaa
    27601 tacagacgga cgagtcgcta ttatgatgcc acaccctgaa cgtgtgttcc gtgcggtcag
    27661 taactcttgg tgcccagaag attggacgga agatggagcg tggatgcggt tgtttagaaa
    27721 tgcgagagtg gcgttgaagt attaaaaatc taccttcgta gtgatctgcc cccatagggg
    27781 gcgattaaaa ttcgaattca tagaacccga atttttatat atctgataaa cgaagttgtt
    27841 tatcctctaa ttcgtgcttt tcctgatatt tcaagtactt tcttatcatt tctgcattga
    27901 catctacggt gtctacgcag tagcctcttg ccacaaaagt gggttgcccc acaatttatg
    27961 cttacgtaaa tagggaaact tattgaaaaa tcttattgga ccctgttcac ccttttaaat
    28021 gccccattac gtcttataca gaaaatgaac gtgatttatt tgaacggtca attctacctt
    28081 aatagaaagt ggtttttagt tagcaatcaa aatcacatta acggaattga gaatttggag
    28141 agtcaggcaa aatgctattt gcgtaaattt aacggcgttc ctaaagtaca tgaattctat
    28201 ttgaaagaat gcagatggcg ttttaatctc agtaacttaa aatatcaagt tttcttttta
    28261 aaacaattgg ttaaattgag tttctaggat agccccttat ttttataatc ctctatttaa
    28321 gtgcatattt ctatttatgc taatcgtcta acgtatcctt ttattataat attgaacatg
    28381 attactattt tcgtcttgtg ctatactaga ctcgcgcaat aaaggcacac ttctttcttc
    28441 agtgtcttta gcgttgcgat ttaaatttaa ttacattttt tataggaaag acaatagaaa
    28501 tgaagatcaa aaaatatgta tttcacctga cttgtacaac acttatctgc cccttaccta
    28561 gggtattcgc agaggaaggt tatatgttag atacggttga ggttaaagcc caagcaataa
    28621 aaaaaacaga tgaagttttc attaaaacag gggcaataag cacccgagat aaaatcttgg
    28681 aatcaaacca aagcttagag actattgttc gttctatgcc tggcgcattt acacaaatgg
    28741 ataaatctca aggtgtggtt tctgttaata ttcgaggagg ttctggattt gggcgagcta
    28801 ataatgcaat aggcccaaat tatatgggaa tgctcgctgg caaataccaa ttcaatgact
    28861 ctacttggat aggagcatta tttgggcata gttggcgcaa aatctctcaa aattataaaa
    28921 taggaggcgg acgaaaagtt acggatagct cgattgacct tagtaaaatt gatgatctag
    28981 ataaagaaag ttctaccacc tcaccgttca atgcagctca tttaaaacaa aaaccaatga
    29041 gtcgtatggc taaattggaa tatggcgata aataccaaac catcacacta ttaccaaagt
    29101 caggtagcgg gtagacattt aaaaaaccaa aacaaccagt taaattatcg ttttcacttg
    29161 cccgagtcac cttggttaga tgttcaagtg gcctatgcgg aaaatcaagg caaacaaaac
    29221 tacaacaaag ggagctgaat taataataaa tatttaattc aaggtttggc aattaacaac
    29281 aaagcgacaa ccttaaattt gagtaacacc tttaaagtgt ctccgtttaa agatggagaa
    29341 tgggaagcaa tattaggttt taatcgttta aagaaccgct atagtaaaaa tagacatcct
    29401 gcggaattaa actataactt agagaatggc gaaaccagcg aaaacaacga ttctgtagga
    29461 atgacagccg ttcgtaaatc gctttatact tcaactttcc agcctgatgg cgaacagaag
    29521 ttcgatactt tttatctcga caatcacttt aactatggta tatggggatt ggatataaac
    29581 gcaaatatta ataccgctcg gtttattggt cagcgtttta aatattttcc ttttgcgatt
    29641 agagaattgg agtcagatat tagacaggca agtagaaata ggcagtttga gcatttaagg
    29701 tcattacaat ctcaattaga caacttgcaa aaaaacattg tacatatatt ccagatgagg
    29761 attttcctaa tgatcctgaa ttgggagagt ttaactgtcc tgatgtcaat tttcctcata
    29821 cagcaacagg aaaacataaa cgaattaatt attcagcaac cttgtctgct tatattcacg
    29881 atttattcac gccctttatt agttattctc aaacacatcg agcccctaat attaaagaaa
    29941 tattcttctc taatttaggt gactatggtg taaataccgc actacaacca gagcaagcga
    30001 aaacaacaca gtttggtatt aatggattca aagaacgggt atttagtgaa gaagataaat
    30061 taggatttaa agtgctattt tataacactc acttaaaaaa ctatatttat aatgtacaac
    30121 ataccgctgt tggtagtgct cctagtttat tcattttcca caaaaactat gaaaaaccag
    30181 taaatatcaa agggatagaa ttagaattta gttatgatat aggctctttc tatggcaact
    30241 tagcttatgc tcgtcaaaat acgaatcaac ccgtatcctt tacagatgca agttcaaggg
    30301 tagatgccgc ttctcattat ggttttaaaa ctcaaggttt tggtgccagc aaaatttcag
    30361 cgttaccaag agactatggc tcattggagt tagggacaag atggtttgat caaagactac
    30421 aaattggcgg agtggcaaaa tattacggaa aaagtaaacg tattgcgata gatgagttta
    30481 actatactta ttatcctgga acacatattg tgaaagaaac tcatcggctt gaagaaactg
    30541 taaataaaca acctattatt gttgattttt acgtgagcta cgaaccaatc aagtcgctta
    30601 tattaaagtt agaactataa aatgtctttg acaaaaagta cattgatcct cttgatgcta
    30661 ataatgatgc agcaagtcag agggtttaca attctgatat cagtgatgaa cctatttata
    30721 cgctcaataa ttatgcaaga gggagaacta tagtgtttag cacaagttat aaattctaaa
    30781 tggggttgtc ctagataagc tagcttaaat ttcctttaac taattatttg gtatagtgac
    30841 taaaattgag tctgcacagt aggttaaaac cttataatat aaggatttaa cccagatggt
    30901 gcaagcgttc acgcttgtgc cagttaattc ctgttctgaa agtatttcag tcataagata
    30961 aaagcctaaa ttagccaagt aaaatttggt ttatttgggc ttttgttttt ggcacaagcg
    31021 tggacgcttg cgtcatcatt ttatttatcc atccattgct atcatttcag tatttctttg
    31081 gcttatcaca agcggttaaa ttttatcaac actttgcaag ttttaatgat ttttgcgatc
    31141 tagatcgaaa aagttatatt ggcttgtgga atttaatttt gaatagggta tttttcttat
    31201 tcctttttaa gttgagaaag gctatgagcc gaaaatataa attccacgaa aagagcggtg
    31261 cttattttgt cagttttgca actgtttatt gggttggtgt gtttgtgaga gagatttatt
    31321 ttcaggtaat tgttgatgcc ttagattact gcagaaaaca taagggaatg attttgtatg
    31381 cctattgtat tatgccaact catatccatt tattatttca agcagaaaat caaaatcctt
    31441 ctgatttaat acgagatttc aaaacattca ccgctaaaat acttattcag agaattaaag
    31501 ataatttgca agaaaaccgt cgggaatggt tgctgtggat gttttctcgg gcggctgaac
    31561 gacgttgtaa tgtaaaacaa tatcaattct ggcaacatca taaccaacct attgaaatct
    31621 attcgcagaa attctttgat gaaaagttaa attatattca tcaaaatcca gtggtaagtg
    31681 gttttgtgtc tgagccttgg gaatggaaat ataattgaaa taaactgctt gtatttttgc
    31741 gatctctgtc gaaaaattgt gaatttttac ttgtctgtcg gagaattttg cgtaaattct
    31801 gcttattatg ttttagttaa aggtattcta tgagtagata acccaaatga aaatagcgat
    31861 ataacctatt tctacccagt aattaattcc cgcttttatg tatcagatca aataatttac
    31921 actgatttat cggtattatc tgtctttagt ttttttaatt aaggagcctt atatgaaatt
    31981 caaaaaatta gcattagcag caatgttagg tggcttattt gtaacagcta atgcggcaga
    32041 attacctaat atcactattt tagcgacagg cggaacgatt gctggtagtc agcagaatgc
    32101 aaatagtagt gcttatcaag cagggcaatt gaatatcgaa accttaattg aagcagtgcc
    32161 agaaatgaaa acattggcga atatcaaagg tgagcagatt gtgaaaattg gcccactaaa
    32221 gccttgttag atgtaggcta tcaaggtatt gtcgtagcag gtgttggtaa cggcaatatg
    32281 aatgcggaaa atcttgcatt atttagagga agccaacaat atttttaaga tttttaataa
    32341 acgttaaagc atataaaaaa tggcataagt gagtcactta tgccattatt tttggggctg
    32401 tcctagataa ctaacctaac ccccctttaa ccaattgttt taaaaggaat atttgagatt
    32461 ttaagtcact gtgattaaaa cgccattcgc attctttcaa atatagctca aaatgggctt
    32521 tcggaat
//
LOCUS       DANJWC010000008        32510 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.008, whole genome shotgun sequence.
ACCESSION   DANJWC010000008 DANJWC010000000
VERSION     DANJWC010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 32510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32510
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.008"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..1942)
                     /locus_tag="PWX02_000241"
     CDS             complement(<1..1942)
                     /locus_tag="PWX02_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDL1728299.1"
                     /translation="MKNRNYFVAIATILALSPEVNATELSDQLASRTRYYPSATDSYY
                     SYYKYAPIYSGYYTSNSRNGYSSWYYPGSNAGVTTRTVSTTVNGITVTKVYTTHNGRT
                     TVRTTYAPAVNKPIVNKPTPIANKPVVDTPTQPVIDANPIMDPPVQPIVDNKPITDTQ
                     PNPITDNKPVIESPIDDTRPVIIRPTTPVNLHPEPVAVGVPNVGIRWNDRSRGYNIDD
                     PNNKQNVKLTGEGVKVGILDSGFKNYLMQDDILKKFGNRAVIIETGRTAPASATHGIE
                     VAEMVGGGNSRNGVASKSTLLLADITKTTNAGTGLSATAPIYYDLWNRGARIFNQSYG
                     IPKPLTYFNNNRNSSYYYLHQFDGNVLQFYKDKVKEGGLFVWAAGNNRGDLDPSLQAG
                     LPHYEADLEKGWISVVAVATRTNQGLGNFEWSNLLPYSQAGVAKNWTVTAMGDYIFNL
                     GGRNIIASGSSFAAPAVTGTAALVKQKYPWMDGNLIKQSILSTATDIGAPGVDDVYGW
                     GLLNIEKATNGPARFDKRLALADNVTVNIPNGSYEFSNNINGDAGLVKNGQGELILSG
                     ASTFEGDTTLNEGAVTINGKKYVSKVNVSPSGTLVVNNTTLENGVNNEGTVVNQGYST
                     IKRAYVASAQSTLVSDLGSNLNV"
     gene            complement(2190..2909)
                     /locus_tag="PWX02_000242"
     CDS             complement(2190..2909)
                     /locus_tag="PWX02_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL1728300.1"
                     /translation="MTQLTELTPALQALRERVLKANLELPKYGLVTFTWGNVSEIDRE
                     TGLVAIKPSGVEYDAMTVEDIVLVDLQGNRVYGDKKPSSDTETHLELYRQFTDIGGVV
                     HTHSRNAVGWAQAGRDLLALGTTQGDYFYGAIPCTRKMTAEEIAGSYELETGKVIVET
                     FRTRGIEPKAVPGVLVHSHGPFTWGKDGHDAVHNSVVLEEVAYMNFISHQIRPQIGPM
                     QQELLDKHYLRKHGANAYYGQ"
     gene            complement(2906..3766)
                     /locus_tag="PWX02_000243"
     CDS             complement(2906..3766)
                     /locus_tag="PWX02_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDL1728301.1"
                     /translation="MRKHKLGIYEKALPKNISWQDRLSIAKVCGFDFVEMSIDETDER
                     LARLDWSKKERAKLVKTIINTGVTIPSMCLSGHRRFPFGSCDEATRQKAYEIMEKAIQ
                     LAVDLGIRTIQLAGYDVYYEEQDEGTIQRFQEGLEWAVELAASNQVTIAVEIMDTQFM
                     SSISRWKKWDEIIKSPWFTVYPDLGNLSAWNDNVAEELKLGIDKISAIHLKDTYKVTE
                     TCKGQFRDVPFGDGCVDFAACFKTLAELNYRGAFLIEMWTEKAEEPITEIINARRWIE
                     QKMQEGGFTL"
     gene            complement(3824..4570)
                     /gene="ulaR"
                     /locus_tag="PWX02_000244"
     CDS             complement(3824..4570)
                     /gene="ulaR"
                     /locus_tag="PWX02_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HDL1728302.1"
                     /translation="MNESYRHKELLALLEERKALSTADIMQLLNISPATARRDISKLD
                     GQGKLKKVRNGAEAILASTVFTAHKITNNAQEKRRIAEAAGRLCKDGESLILTCGSTM
                     EMLGEVLCGRKLQIITNYLPLANQLIAGCHDDVVIMGGQYNKHKKVMLSINTQNEMTY
                     AATTMFTSGKGLTSEGLFKTDMIIANAEQQMAKHAERYVVLLDSTKLGKKVGMLFKEF
                     DKIDLLITGKEADPTIIQELRNRGLDIILA"
     gene            complement(4654..5745)
                     /gene="ulaG"
                     /locus_tag="PWX02_000245"
     CDS             complement(4654..5745)
                     /gene="ulaG"
                     /locus_tag="PWX02_000245"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDL1728303.1"
                     /translation="MSKIQEITRESWILSTFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     CVGVWIKTPAGANICMDLWCGRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRAQPMVL
                     DPFAINEVDFILASHYHSDHIDVNVVAAICNNPKLDHVKFVGPWHCAELWKKWGVPEE
                     RIIVVKPGDVVKLKDVEIHALDSFDRTCLVTLPVEGAEERGGELAGLCPSDEEMGRKA
                     VNYVFKTPGGTIYHGADSHYSIQFAKHGKQFDIDVALNNYGENPVGIADKMTSVDLLR
                     MAECLRCKVIIPVHHDIWTNFMASTQEIIDLWRMRKDRLQYQFHPFIWEVGGKYVYPR
                     DKDLIEYHHPRGFDDCFEQEPNIQFKSML"
     gene            6128..7923
                     /locus_tag="PWX02_000246"
                     /pseudo
     CDS             6128..7923
                     /locus_tag="PWX02_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249346.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate-specific subunit IIBC"
     gene            8003..9064
                     /locus_tag="PWX02_000247"
     CDS             8003..9064
                     /locus_tag="PWX02_000247"
                     /inference="COORDINATES: protein motif:HMM:NF012376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL1728304.1"
                     /translation="MKKILSTLFMLSAVSVAVAKEVNIKLLGTSDVHGRVVPWSYGAD
                     VEDKSGSFAQIATYVKEVRANHKNVLLFDIGDAIQDNQVEEFAKTLDKAKNNPVPKVL
                     NAMNYDIFILGNHEFNFGMPNLDAILKDINAKILTANFYHKDGKRYVEPTTIIERDGV
                     KLGIIGLTTPMSAIFEKDTGHLDAMKFTSPIEEAKAQIADLKAKGVDAIIVLAHMGIE
                     NENNIPDTGVADLVNAVDGIDVIIAGHMHKDVPSETIKNTLITEPHRYGTVVSEVDLA
                     FDVNDKGEVKLLSKTAKTVPVKKLASDPEIEKIYQPYHNELRALNNVKIGETAQDLIP
                     QGKITVYRLLSRKTPECPH"
     gene            9052..9516
                     /locus_tag="PWX02_000248"
     CDS             9052..9516
                     /locus_tag="PWX02_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HDL1728305.1"
                     /translation="MSSLIHDVQQHYSGADVVSFAFDYETVRLDKGDIKKKDIIYNYR
                     YAGGDVSMYELTGKQLKQYMEWSADYFDTIQAGDKDYRYNAVRGKSKYVTFDLFGGVS
                     YNIDLRNPSGSKIVDLKLANGSLITDDGKYKVGMNSYRFGQLTKKGGIWEGQ"
     gene            9547..9663
                     /locus_tag="PWX02_000249"
     CDS             9547..9663
                     /locus_tag="PWX02_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ser/Thr protein phosphatase"
                     /protein_id="HDL1728306.1"
                     /translation="MGAEKGTIQNMLIDYITNVKGGKIEGKSHNRWAIIGLN"
     gene            9679..10146
                     /locus_tag="PWX02_000250"
     CDS             9679..10146
                     /locus_tag="PWX02_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1728307.1"
                     /translation="MNLKQSLIENNSILLNQSAANWEDAIKIGTDLLVKAGTIEPRYY
                     DNIVSKIKEMGPYIILAPGLAMPHARPEEGVIKTSFALVTFDTPIYFEGEDEPVHVML
                     TLAGSDSDQHMQGLVEITQVLDDPESDTGVDLDKIRRCKTAEEVYAVIDQALA"
     gene            10201..10881
                     /locus_tag="PWX02_000251"
     CDS             10201..10881
                     /locus_tag="PWX02_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDL1728308.1"
                     /translation="MSKPLLQIALDSLSLEKAVADAKQAENQVEIIECGTILAFAEGM
                     KAVSTLRALHPNHIIVCDLKTTDGGAILAKMAFEAGADWLTVSAAAHPATKAACKKVA
                     DEFNAAHPELKVKKEIQIEIYGNWTVEKDAKEWVELGVTQAIYHRSRDAELAGKGWTT
                     EDVELMKQLSDLGIELSITGGIVPEDIHLFKEIKNAKAFIAGRALVGEKGKQTAEAIR
                     VEIDKYWN"
     gene            complement(10949..12091)
                     /locus_tag="PWX02_000252"
     CDS             complement(10949..12091)
                     /locus_tag="PWX02_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL1728309.1"
                     /translation="MKIVIAPDSFKESLTALEVAQAIQQGFARVFPEAEYQLVPMADG
                     GEGSVQSLVDATHGSLQKISVIAPLANEVEGFFGLSGDQKTAFIEMAAASGLHLVPME
                     QRNPLVTTSYGTGQLIKAALDLGVEKIILGIGGSATNDGGVGMLQALGAKFLNAEQRE
                     IGFGGEALSQIQQIDLSGLDSRLKQVEFEVACDVDNPLCGERGASAIFGPQKGATAEM
                     VKTLDHALLHFAETVKSQLGIEIAEKAGAGAAGGMGGGLLLLPNVTLRSGVQIILEAT
                     DLAEKLADADLVVTGEGRMDAQSIAGKTPIGVAKVAKQFGKPVIAIVGSLKEDYPVVY
                     QHGIDAVFPIIRQVSNLEAVLKSGKENLISTAENVARLWKISASQE"
     gene            complement(12101..13480)
                     /locus_tag="PWX02_000253"
     CDS             complement(12101..13480)
                     /locus_tag="PWX02_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDL1728310.1"
                     /translation="MSGIALILIFLVAIIIMIYMIAKWKVHPFLALMSISIALALVAG
                     LPLAKIPTIVGEGFSNTFKSIGIVIIFGAIIGTILEKTGAALKLADMVVKVVGQKRPE
                     LAMLIMGWVVGIPVFCDSGFVVLNPIREAIRKKILANPVGMAVALSAGLYASHVFIPP
                     TPGPIAAAGAVGLSHNLLLVIGMGFVVSIPVLIASYFFAKYVGKQVSVMEEAQADQVI
                     SQSYEDLIKQYGKLPSGFLSLAPIFMPIILMAIGSIAKIAGVSGDFGVFLQFVGNPII
                     ALAIGVIFAIFLLVSSGKMAEFDSLTNDTLKLVGPILFITAAGGVLGNVITQAGFVDY
                     IKQNAHIISTAGIFFPFLISAILKTAQGSSTVAIITTASIMGMFNAPDSLMNALGLTS
                     EMAAALTVMAISAGSMCVSHANDSYYWVVTNFTKLTPQQGYRTQTTLTFIMGVVGMLT
                     VYLLSLILL"
     gene            complement(13602..14696)
                     /locus_tag="PWX02_000254"
     CDS             complement(13602..14696)
                     /locus_tag="PWX02_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1728311.1"
                     /translation="MKLNQAIADRIVKRTMQIIPNSVNVMDSEGVIIASGDVGRIGQR
                     HTGAVIALRNDQAVEIDENLAKQWCDEVKAGINLPLHYLGSTIGVVGISGQPQQVRQY
                     AQLVKMAAELIMQQAFELEQERWQRRYREEFVRALLKGSLSTAEIQQQAVFFGQDFAK
                     PLSVMVIKIHGSTAEKLQRLIDFFEHYFPSLLTAVIGLDKIALLNHLSNEKSLIRNAL
                     NKLNDPDISFKTVVGLAVENLSQTHISYQTACHTLNYAEQIQSRKQIIHFEDFKLPAL
                     LHSFSHSWEAKTLLSPFQALLVSDKKQVLLKSLRYYFFANCDLDHAAKKLFIHPNTLR
                     YRLEKIEQITGLSFNNIEQKFVLYLGAVLS"
     gene            complement(14800..15252)
                     /locus_tag="PWX02_000255"
     CDS             complement(14800..15252)
                     /locus_tag="PWX02_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HDL1728312.1"
                     /translation="MNLKAVAVSALAMSSVAMADIADVKPVVFESKFDFQTTVTTLTN
                     TLKEKGMTIFAEIDHQAAAKQAGLEMQPATVLVYGTPKAGTPLMVKDPMLALQLPLKV
                     LITEPNKGKVEVMLNRADQVVAHANTDYADVENNLAKAEKLIQATVAK"
     gene            complement(15273..16025)
                     /locus_tag="PWX02_000256"
     CDS             complement(15273..16025)
                     /locus_tag="PWX02_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDL1728313.1"
                     /translation="MHFEKRSHWSGELGREMSFNVYGHAGKPVIVFPSSGGNENEYGN
                     FGMIEACRSFIERGLIKFYTPDSYDAESWLSKHKSGHDMALAHNAYDRYIIHELVPLI
                     RHESHWNGTMIATGCSMGAFHTVNFALRHPDLFDIAIALSGVYDARFFSGEFYGDKAV
                     YFNSPIDYVWGQNDDWFLDRYRQNHYVIAVGQGAWEEQHIEDTRRLQEAFNAKGIDAW
                     FDYWGFDIDHDWPTWRKQMPYFLGKLEEQGII"
     gene            complement(16036..17220)
                     /locus_tag="PWX02_000257"
     CDS             complement(16036..17220)
                     /locus_tag="PWX02_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDL1728314.1"
                     /translation="MSRPLNFVMISPHFPTNFETFAIRLREAGFNTLGIADAPYESLS
                     ENLRHSLTEYYRVDNMEDYEQVYRAVAYFAHKYGRIDRIESHNEYWLELDAKLRTDFN
                     VFGYKSDDMLSIKTKAQMKEVFRKTGLKVAQGRVFKDDEDACKLAKQLKFPVIIKPNS
                     GVGASDTYKIKTAEELEAFFGYKTPTVEYIMEEFIDGDIITFDGLTDKEGNIVFYSSL
                     EYSEAVLDTVSKDGDMFYYVPCEISPKLVEMGKQCVKAFNVKERFFHFEFFCVKGSKK
                     EELLPLEINCRPPGGLTIDMWNYANDFDVFREFANIVKENKFYADIQHPWNVVYISRK
                     ANQNYVNSIEAVCEKFAGNIISVQSVPGVFAKVMGEQGILARTETMEQMREIAQFAQA
                     KQ"
     gene            17513..17944
                     /locus_tag="PWX02_000258"
     CDS             17513..17944
                     /locus_tag="PWX02_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS sensor domain-containing protein"
                     /protein_id="HDL1728315.1"
                     /translation="MAEQIVPSNEAVLKAGHDTLVISTTDLQGVITYANDLFCKLTGF
                     TREELIGQPHNIVRHPAVPKEVYKDMWDTIQAGKIWTGIVPNCGKGGVVYVVDTTVQP
                     MFDETGKITGYISVRRVINDLMTNFEAVESAKEIFDDFYGE"
     gene            18015..19571
                     /locus_tag="PWX02_000259"
     CDS             18015..19571
                     /locus_tag="PWX02_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase flavoprotein subunit alpha"
                     /protein_id="HDL1728316.1"
                     /translation="MSLIPHIHIAYGSESGNAEKLAQQLAIQPFLLPYSPTLSTLNET
                     DLSQLSAESLLLIVTSSFGDGEPPENADEFAEKLENLTACNLQYAIFGLGDITYDKFC
                     GYSKQLDSLLQQKQAKAVIARVDADLNYQEIFKQWLPLVEQALHSTNKETIQHSLSVQ
                     AYGEEMAYQAEMLEIKHLANSSPAVYHIRLSLKESGIFYQAGDLIYVKVQQPDRLLNE
                     YAEWFNDQNAVALLRHKELRLLSKNVLRDIQKICGSNELKDLTKISNKKALEQYVYGR
                     DLLDVLRDFDPNKSVTLADLEPILSNLAARAYSISSCGKTHPDYVDLCIRHVSYHLNG
                     RDYQGTASGYLSELQAGDFVSIFANANPNFRLPETLTAPVVMIGSGTGIAPHIAFLQA
                     LEQQYQGVESYLFFGERYRDKDFLYQTELENYLAKGTLTQLFTAFSRDQAEKFYVQDA
                     LSQQAELVWHLIQQGAYFYICGSKAMSKAIDAELIKIADQIGGEPYIDDFNNIIAKLV
                     AEGRLMRDVY"
     gene            complement(19629..19886)
                     /gene="rpsQ"
                     /locus_tag="PWX02_000260"
     CDS             complement(19629..19886)
                     /gene="rpsQ"
                     /locus_tag="PWX02_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005759643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDL1728317.1"
                     /translation="MTDKIRTVQGKVVSDKMDKSFVVAIERTVKHPLYGKFIRRTTKL
                     HVHDENNEAKLGDVVEIKECRPISKTKSWTLVRVVEKAVVA"
     gene            complement(19886..20077)
                     /gene="rpmC"
                     /locus_tag="PWX02_000261"
     CDS             complement(19886..20077)
                     /gene="rpmC"
                     /locus_tag="PWX02_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDL1728318.1"
                     /translation="MKAQELRNKSVEELNGELVNLLGEQFKLRMQAATGQLQQTHQLK
                     QVRRSIAQVKTVLTEKAGE"
     gene            complement(20077..20487)
                     /gene="rplP"
                     /locus_tag="PWX02_000262"
     CDS             complement(20077..20487)
                     /gene="rplP"
                     /locus_tag="PWX02_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDL1728319.1"
                     /translation="MLQPKRTKFRKVHKGRNRGIAGGTEVSFGSFGLKAIGRGRLTAR
                     QIEAARRAMSRAVKRQGKIWIRVFPDKPITEKPLEVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVSEEVARNAFALAAAKLPFKTTFVTKTVM"
     gene            complement(20501..21208)
                     /gene="rpsC"
                     /locus_tag="PWX02_000263"
     CDS             complement(20501..21208)
                     /gene="rpsC"
                     /locus_tag="PWX02_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDL1728320.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTQDFADNLDGDFKVRKFLN
                     KELANASVSRITIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRNAVAKIAGVPAQIN
                     IAEVKKPELDAKLVADSIASQLERRVMFRRAMKKAVQNAMKLGAKGIKVEVSGRLGGA
                     EIARSEWYREGRVPLHTLRADIDYSTAEAHTTYGVIGVKVWIFKGEILGGMAAVIESE
                     KEPAAQPKKAPRGKGRK"
     gene            complement(21228..21560)
                     /gene="rplV"
                     /locus_tag="PWX02_000264"
     CDS             complement(21228..21560)
                     /gene="rplV"
                     /locus_tag="PWX02_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDL1728321.1"
                     /translation="METIAKHRYARTSAQKARLVADLIRGKKVSVALEILTFTNKKAA
                     TLVKKVLESAIANAEHNDGADVDDLKVAKIFVDEGPSMKRVMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            complement(21571..21846)
                     /gene="rpsS"
                     /locus_tag="PWX02_000265"
     CDS             complement(21571..21846)
                     /gene="rpsS"
                     /locus_tag="PWX02_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDL1728322.1"
                     /translation="MPRSLKKGPFLDLHLLKKVEKAVESGDKKPIKTWSRRSMIIPSM
                     IGLTIAVHNGRQHVPVYVSDEMIGHKLGEFAPTRTYRGHAADKKAKK"
     gene            complement(21877..22698)
                     /gene="rplB"
                     /locus_tag="PWX02_000266"
     CDS             complement(21877..22698)
                     /gene="rplB"
                     /locus_tag="PWX02_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDL1728323.1"
                     /translation="MAIVKCKPTSAGRRHVVKVVNAELHKGKPFAALLDTKSKTGGRN
                     NLGRITTRHIGGGHKQHYRLIDFKRNKLDIPGVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLTAGDQIQAGASAPIKVGNALPMRNIPVGTTVHNVELKPGKGGQIARSAGA
                     YVQIIAREGNYVTLRLRSGEMRKVLAECTATIGEVGNSEHMLRVLGKAGANRWRGIRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRHNKRTDKFIVRRRGK"
     gene            complement(22717..23022)
                     /gene="rplW"
                     /locus_tag="PWX02_000267"
     CDS             complement(22717..23022)
                     /gene="rplW"
                     /locus_tag="PWX02_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDL1728324.1"
                     /translation="MIQQERLLNVLKAPHISEKATNNAEKGNTIVFKVALDANKVEIA
                     NAVEELFEVKVDSVRTVVVKGKTKRRGAKVGRRSDWKKAYVTLQEGQSLDFVEGAAE"
     gene            complement(23019..23621)
                     /gene="rplD"
                     /locus_tag="PWX02_000268"
     CDS             complement(23019..23621)
                     /gene="rplD"
                     /locus_tag="PWX02_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDL1728325.1"
                     /translation="MELQVVGANALTVSETTFGREFNEALIHQVVVAYAAGARQGSRA
                     QKTRAEVSGSGKKPWRQKGTGRARSGDIKSPIWRSGGVTFAAKPQDHSQKVNKKMYRG
                     AIKSILSELVRQDRLVVVEKFEIDAPKTKVLAQKLKDMALNDALIITANVDENLFLAA
                     RNLYKVDVRDVQGIDPVSLIAFDKVVVTVDAVKQIEEMLA"
     gene            complement(23637..24266)
                     /gene="rplC"
                     /locus_tag="PWX02_000269"
     CDS             complement(23637..24266)
                     /gene="rplC"
                     /locus_tag="PWX02_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDL1728326.1"
                     /translation="MIGLIGRKVGMTRVFTEDGVSIPVTVIEIEANRVTQVKTLENDG
                     YSAIQVTTGSKKASRVTKPEAGHFVKAGVEAGRGLWEFRTTEGEEFTLGQEINVDIFT
                     DVKKVDVTGTSKGKGFQGGVKRWNFRTQDATHGNSLSHRVLGSIGQNQTPGRVFKGKK
                     MAGHLGAERVTVQSLEVVRVDVERKLLLVKGAVPGATNGDVIVKPAVKA"
     gene            complement(24292..24603)
                     /gene="rpsJ"
                     /locus_tag="PWX02_000270"
     CDS             complement(24292..24603)
                     /gene="rpsJ"
                     /locus_tag="PWX02_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDL1728327.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQSTAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHKRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            25047..25688
                     /locus_tag="PWX02_000271"
     CDS             25047..25688
                     /locus_tag="PWX02_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family DNA transformation protein"
                     /protein_id="HDL1728328.1"
                     /translation="MQTINEIKEEIYPIFEPIIGTIRLKTYFSYYAVFKDGLMIALYQ
                     NGATYLRIAKKDIEYIRQYPETCILCDEKIGIQSRKFHYIPQVLIDDTPKFSSLLFGI
                     IEELRTEKYKISQKRATQIRNLPNMNLKLERMLKKIGIHSIHDFTQEGYMSTFIKLIL
                     QGFDVTEDLLFKLNGALNHQYIYTFTDQQKRELMQEANQALYNSGLRKRFNAL"
     gene            25849..27243
                     /gene="fumC"
                     /locus_tag="PWX02_000272"
     CDS             25849..27243
                     /gene="fumC"
                     /locus_tag="PWX02_000272"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005567406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDL1728329.1"
                     /translation="MEFRIEKDTMGEVQVPADKYWAAQTERSRNNFKIGPEASMPAEI
                     IEAFGYLKKAAAYANHDLGVLAVEKRDLIAKACDEILANKLDDQFPLVIWQTGSGTQS
                     NMNVNEVVANRAHVLNGGKLGEKSIIHPNDDVNKSQSSNDTYPTAMHIAAYKKVVEHT
                     LPCVKRLQKTLAEKAEAFKDVVKIGRTHLMDATPLTLGQEFSAYAAQLAFGIKALENT
                     LPHLRELALGGTAVGTGLNTPQGYDVKVAEYIAKFTGLPFVTAQNKFEALATHDAIVE
                     THGALKQLAVSLYKIANDVRLLASGPRSGIGEILIPENEPGSSIMPGKVNPTQCEAMT
                     MVCAQVLGNDVTISFAGTQGHFQLNVYKPVMAYNFLQSAQLLGDACVSFDEHCAVGIE
                     PNYPRIKQQLDQSLMLVTALNTHIGYENAAKIAKTAHKNGTTLKEEAINLGLVTAEQF
                     DEWVRPEDMVGSLK"
     gene            27370..27480
                     /locus_tag="PWX02_000273"
     CDS             27370..27480
                     /locus_tag="PWX02_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005696979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL1728330.1"
                     /translation="MKKLILIALVTTLGLSACGVKGPLYFPEQAPKHEQK"
     gene            27585..28835
                     /gene="lysA"
                     /locus_tag="PWX02_000274"
     CDS             27585..28835
                     /gene="lysA"
                     /locus_tag="PWX02_000274"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDL1728331.1"
                     /translation="MNHFEYKNNQLFAEEVAIADIIEQYGTPAYVYSRSTLERHLHAF
                     NNAFGSHPHLVCFAVKSNSNIALLNVMARLGSGFDIVSQGELERVLAAGGEASKVVFS
                     GVAKSHSEIKRALEVGIRCFNIESVSELHRINQVAGELGKIAPISLRVNPDVDAHTHP
                     YISTGLKENKFGVSVTQAREVYRLAKTLPNVKITGMDCHIGSQLTELQPFLDATDRLI
                     ILMEQLREDGIELHHLDLGGGLGVPYNGEEPPHPTEYAKALLEKLKGYENLEIIIEPG
                     RAITANAGILVTKVEYLKQNEDRNFAIVDTGMNDMIRPALYEAYMQITEVDKTLNRDK
                     AVYDVVGPICETSDFLGKQRELAISEGDLIAIRSAGAYGAAMSSTYNSRPQAAEIMVD
                     GNQAHLIKARATFADLWRLEKLLP"
     gene            28849..29478
                     /locus_tag="PWX02_000275"
     CDS             28849..29478
                     /locus_tag="PWX02_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HDL1728332.1"
                     /translation="MKLYGLTGACSFVPHVALEWVKLRANQDYAFQAVSREFIKSAEY
                     LALNPRGNVPLLVDGDLALTQNQAIVHYLDELYPEAKLFGSKTVRDKAKAARWLAFFN
                     SDVHKSFVPLFRLPSYAEGNETLTKTIRQQSAEQILEQLAFANAHLENHIFFGEEISV
                     ADAYLYIMLNWCRLLGLDFSHLSQLSAFMQRVEADQGVDNVREQEGLKG"
     gene            29549..30994
                     /gene="tldD"
                     /locus_tag="PWX02_000276"
     CDS             29549..30994
                     /gene="tldD"
                     /locus_tag="PWX02_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HDL1728333.1"
                     /translation="MLNQVSNSLLAPSGLELSHLTQVLDHFSGRHIDYADLYFQLSQD
                     ESWSLEDSIIKEGGFYIDRGFGVRAISGEKTGFAYADQITLKQLEQCAAAARSISPTS
                     GQLSVKNFKKTTACSRYPAINPLETISREQKVELLHLVDKTARMADPRVIQVNANLSA
                     VYEEMLVAATDGTLAADIRPLVRLSVSVLVEQNGKRERGSAGAGGRFGLDWFLAPSET
                     GFSRAESLTKEAVRQALVNLTAIPAPAGTMPIVLGAGWPGILLHEAVGHGLEGDFNRK
                     ESSLFSGKIGQMVTSELCTIVDDGTVPNMRGSITVDDEGVPSQRNALIENGILKGYMQ
                     DKLNARLMGVAPTGNGRRESYAHLPMPRMTNTYLTEGNHEFEEMIESVEYGLYAPHFS
                     GGQVDITSGKFVFSTAEAYLIEKGKITKPVTGATLIGSGIEAMQQVSMVGKKMELDPG
                     IGTCGKEGQSVPVGVGQPTVKLDKITVGGRG"
     gene            31058..31939
                     /gene="prmA"
                     /locus_tag="PWX02_000277"
     CDS             31058..31939
                     /gene="prmA"
                     /locus_tag="PWX02_000277"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDL1728334.1"
                     /translation="MAWVQIRLNSTDKQAEEISDFLTEIGAVSVTFMDSQDTPIFEPL
                     PGETRLWGNTDVVGLFDAETDMNLIVEALKTSKLVDENFAHKIEQIEDKDWEREWMDN
                     FHPMQFGKRLWICPSWREVPDPNAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLEGK
                     TVIDFGCGSGILAIAALKLGAKQAIGIDIDPQAILASQNNAEANGVAEKLQLFLAKDQ
                     PRELQADVVVANILAGPLKELAPNIITLVKPNGNLGLSGILATQAESVCEAYSEAFTL
                     DPVVEKEEWCCITGRKI"
     gene            complement(32114..32189)
                     /locus_tag="PWX02_000278"
     tRNA            complement(32114..32189)
                     /locus_tag="PWX02_000278"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32154..32156),aa:Trp,seq:cca)
     gene            complement(32256..32332)
                     /locus_tag="PWX02_000279"
     tRNA            complement(32256..32332)
                     /locus_tag="PWX02_000279"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32296..32298),aa:Asp,seq:gtc)
     gene            complement(32343..32458)
                     /gene="rrf"
                     /locus_tag="PWX02_000280"
     rRNA            complement(32343..32458)
                     /gene="rrf"
                     /locus_tag="PWX02_000280"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         9444 a         7206 c         6528 g         9332 t
ORIGIN      
        1 atacatttaa gtttgaacct aaatctgaca ctagcgtact ttgtgctgaa gcaacgtaag
       61 ctctcttaat cgtagaataa ccctggttga caactgtacc ctcgttattt acgccatttt
      121 ctaacgtggt attattgaca accaatgtac cacttggaga tacgtttact ttagacacat
      181 attttttacc gttaatagtg acagcacctt cattgagtgt tgtatcccct tcaaaagtac
      241 tagcacccga taaaattaat tcgccttggc catttttcac caagcctgca tcaccgttaa
      301 tattattgga aaactcatag cttccatttg gaatatttac agtcacgtta tcggctaaag
      361 cgagtcgttt atcaaaacgc gcagggccat tagtggcttt ttcaatatta agtaaacccc
      421 atccatatac atcatcaact ccaggagcgc cgatatcggt tgccgtcgag agaatagatt
      481 gtttgataag gttaccatcc atccaaggat atttttgttt cacgagagct gctgtacccg
      541 taacggctgg agctgcaaag gatgaaccac tagcaataat gtttctacct cctaaattaa
      601 agatgtagtc acccattgcg gtaacggtcc aattttttgc cacaccggct tgagaatatg
      661 gcaataaatt gctccattca aagttaccca atccctgatt agttctggtt gctaccgcaa
      721 caacagaaat ccaacctttt tccaagtcag cttcataatg aggtaaccca gcttggagtg
      781 atggatcaag atcaccacgg ttattccccg cagcccagac aaataagccc ccctctttta
      841 ctttatcctt atagaattgc aacacattac catcaaattg gtgtaaatag taatagctag
      901 aatttctatt gttattaaaa taagtaagag gctttggaat accgtatgat tggttaaaaa
      961 tacgggctcc cctattccat aagtcataat aaattggagc tgttgcggat aatcctgtac
     1021 ccgcatttgt tgttttggta atatccgcaa gtaataacgt tgacttagac gctactccat
     1081 tgcgagagtt ccctccccca accatttctg cgacctcaat accatgtgtt gcagaagccg
     1141 gggcagtacg ccctgtttca ataataacag cgcgattacc aaattttttg agtatatcgt
     1201 cctgcattaa atagttttta aaaccactat ctaagatacc gactttaaca ccttcaccag
     1261 ttaacttaac attttgtttg ttattaggat catcaatgtt gtaaccacga gagcggtcat
     1321 tccatcgaat acctacattg ggtacaccaa cagctacagg ttcaggatga agatttaccg
     1381 gtgttgtagg cctaatgata actggacggg tgtcatcaat tggcgattca atgacaggtt
     1441 tgttatctgt aattggatta ggctgtgtat cagtgatggg tttattatca actattggtt
     1501 ggactggcgg atccataatt gggttggcat caataaccgg ttgagttggc gtatctacaa
     1561 cgggtttatt ggcaatcggt gtcggtttat ttacaattgg tttgttcaca gcaggagcat
     1621 aagtggtacg aacggttgtt ctcccattat gcgttgtata aacttttgta actgtaatgc
     1681 cattaactgt tgtcgaaaca gttctggttg ttacacctgc attgctacct ggataatacc
     1741 acgacgaata accattacgg ctattcgatg tatagtagcc cgaataaatc ggtgcatatt
     1801 tatagtaact ataataacta tccgtggcgg aaggataata tcttgttcta cttgcaagct
     1861 gatctgaaag ttcagttgcg ttaacctctg gcgataaggc taatattgtt gctattgcaa
     1921 caaaataatt tctatttttc accttttggt ttccttctaa taaatacaaa gatactcttg
     1981 tttatttcaa atctactacg gatccttaat agacttgtaa aaacaagagg cagagattag
     2041 aacaaaaatc aaaattaaaa tcaagaaaag gaaaatggta attatttgtg gatctatcca
     2101 taaactcaaa cagactgagg agctattccg attatagaaa gcccctcgaa agctgttgat
     2161 aatttggaaa ataattttta ggttattagc tattgcccat aataagcatt tgcaccatgc
     2221 ttacgcagat aatgcttatc taataattcc tgttgcattg ggccaatttg tgggcgaatt
     2281 tgatggctaa taaaattcat gtaagccacc tcttctaata ccacagaatt atgcacagca
     2341 tcgtgtccat cttttcccca ggtaaatgga ccgtgtgaat gtaccaacac acctggaact
     2401 gctttcggtt caatacctcg agttcggaaa gtttcgacaa tcacctttcc ggtttccagc
     2461 tcataactgc cggcaatttc ctctgcggtc attttgcgag tacaaggaat cgcaccgtag
     2521 aaataatcgc cttgagtggt gccaagtgcc aataaatcac gccctgcttg tgcccaacct
     2581 acagcattgc gagagtgagt gtgtaccaca ccgccaatat cagtgaattg gcgataaagt
     2641 tctaagtgcg tttctgtatc agaagacggt tttttatctc cgtaaactcg attaccttgt
     2701 aaatcaacca atacaatgtc ttccaccgtc atcgcatcgt attctacgcc ggaaggtttg
     2761 atggcgacta aaccggtctc acggtcgatt tcacttacgt taccccaagt gaaagttacc
     2821 aagccatact tcggtaattc taaattggct tttaacacac gctctcttaa ggcttgtaat
     2881 gcaggggtta attcagttaa ttgcgtcata gggtaaatcc tccttcttgc attttttgtt
     2941 cgatccaacg gcgggcgttg atgatttcag taatcggctc ttcggctttt tccgtccaca
     3001 tttcaattaa gaatgcgcca cggtaattca gctcggcaag ggttttaaaa caagcggcga
     3061 aatctacgca accgtcaccg aatggtacgt cacggaactg acctttgcag gtttcagtca
     3121 ctttgtaagt gtcttttaag tgaatagctg agattttgtc gatgccgagt ttcagctctt
     3181 ccgctacgtt gtcgttccac gctgagaggt tgcctaaatc cggatacacg gtgaaccacg
     3241 gtgatttgat gatctcgtcc cattttttcc agcgggaaat cgaactcata aattgggtgt
     3301 ccataatctc taccgcaatg gtgacttggt tgcttgccgc cagctctacc gcccattcta
     3361 aaccttcttg gaagcgttga atcgtgcctt cgtcctgctc ttcgtaataa acgtcatagc
     3421 cggcaagctg aatggtgcga ataccgagat ctaccgccag ttgaatggct ttttccataa
     3481 tttcataggc tttttggcgg gtcgcttcat cacaagaacc aaacgggaaa cggcgatgac
     3541 cggacaagca cattgacgga atggtcacgc cggtgttgat gatggttttc accagcttgg
     3601 cacgctcttt tttgctccaa tcaaggcggg ctaagcgttc gtccgtttcg tcaatcgaca
     3661 tttccacaaa gtcaaaaccg cagactttgg cgatagaaag gcggtcttgc cacgaaatgt
     3721 ttttcggcaa ggctttttcg taaataccaa gtttatgttt tctcatagtt ttcccttgaa
     3781 catcataagt aaaattttga ttaaatttga ccgcttgtac aggctaagcc aagataatat
     3841 ccaagcctct atttcgcaat tcttggataa ttgtcggatc agcttctttg ccggtaatca
     3901 ataaatcaat tttatcgaat tctttaaaca gcataccgac ttttttaccg agcttggtgc
     3961 tatccaataa caccacataa cgttctgcgt gctttgccat ttgttgctcg gcattggcaa
     4021 taatcatatc ggttttaaat aatccttcgc tggttaagcc tttgccactg gtaaacattg
     4081 tggtagcggc ataagtcatc tcattttggg tgttaattga gagcatcact tttttatgtt
     4141 tattatattg cccacccata atcactacat catcgtggca acctgcaatc aactggtttg
     4201 caagcggtag ataattggta ataatttgca attttctacc acataatact tcaccgagca
     4261 tttccattgt ggaaccacac gtcagaataa gactttcccc atctttacat aaacgaccgg
     4321 cggcttccgc tattctacgt ttctcctgcg cattgttagt tattttgtgt gctgtaaaaa
     4381 ctgtagaagc tagaattgct tctgctccgt ttctaacctt ttttaattta ccttgaccat
     4441 ctaatttact gatatcacgt cttgctgttg ccggagaaat atttaacaac tgcataatat
     4501 ctgctgttga aagtgctttc cgttcttcca acagggctaa taactctttg tgtctataac
     4561 tttcgttcac tactttatcc cattactaat cactactgct ccataatgaa cttgctaaaa
     4621 cgccactctc cagtgagagt ggcgactgct cacctatagc atggatttaa attgaatatt
     4681 tggctcttgc tcgaagcagt catcaaagcc gcgagggtga tgatattcaa ttaagtcttt
     4741 atcacgtggg taaacatatt tgccgcccac ttcccaaata aatgggtgga attggtattg
     4801 taagcgatct ttacgcatac gccataaatc aataatttct tgcgtacttg ccataaagtt
     4861 tgtccaaata tcatggtgaa ctggaataat cactttacag cgtaagcatt ccgccatacg
     4921 taataaatca actgaagtca ttttatccgc aataccaacc gggttttcgc catagttatt
     4981 cagtgctacg tcaatatcaa attgcttacc gtgtttagcg aattggattg agtagtgaga
     5041 atccgcaccg tgataaatcg taccgcccgg ggttttgaac acatagttca cagctttgcg
     5101 tcccatttct tcatctgatg ggcataagcc tgcaagttct ccgccacgtt cttctgcacc
     5161 ttctaccggt aaggtcacta aacaagtacg gtcaaatgaa tctagggcgt gaatttctac
     5221 atcttttaat ttcacgacat cgcccggttt taccacaatg atacgctctt ccggcacgcc
     5281 ccattttttc cataattctg cacagtgcca cgggccgaca aatttaacgt ggtctaattt
     5341 cggattattg caaatagccg ctaccacgtt tacatcaata tggtctgagt gatagtgaga
     5401 agcgagaatg aagtccactt cgttaatcgc aaacggatct aacaccatcg gttgagcccg
     5461 caaattaggc tggagcttac gcacccctgc catatttgcc atttgatgac cacgcaccat
     5521 atcttttact ttcttggtgg atttaccacg accgcaccat aaatccatac agatattcgc
     5581 accagctggg gttttaatcc acacaccgac acagcctagc caccacattg caaaattgcc
     5641 ttccggtaca acttcttctt caatttcttc attcaaccaa gtaccccatt cagggaaagt
     5701 tgaaaggatc cagctttcac gagtgatttc ttgaattttg ctcataatag tctcctgatt
     5761 tacgctaaaa taagaacaat gcaatcaaaa ccaatcataa acaatcacaa aaatttgtac
     5821 atctttttta ttcaaaaatg tgatctatgt ctaatttttt atgatttact ggtaaaaatt
     5881 tattttaata acttgattta aataccattt atttagcata tatgagctaa tttaaagaat
     5941 aatctttaat aatcacgaat aaatatattt ttcttcctcc atgacgagat tataaaaata
     6001 aaatgtgatc tcgctcacaa ataattaaaa ataatctttt ttaatctttt tctagcgttt
     6061 atcattaatt ccgacaactt ctctgaagtt ttttagttct attaatctct caacaaagag
     6121 gccccctatg gactttttaa ccgaaattct ccttttctta aaggatcaag tgctgaataa
     6181 agcacccttc ttactaggta tcgtagcttg cttaggttac attcttctta aaaaagacac
     6241 ttctacgatt attaaaggca caattaaaac aattgtcggt tttatgattg tacaagccgg
     6301 ttcaggtttc ttagtaacca actttaagcc ggttattgat ggcttagcca aatatcacca
     6361 acttaccggt gcagtaattg acccttacac cagtatgcaa tcgaccattc aaaccatggg
     6421 cgagaactac gcttgggttg gttatgccgt aattggggcg ctgttcctca atattctatt
     6481 agtggtgttc cgccgtttaa ccggtatccg taccattatg ctcaccggtc acattatgtt
     6541 ccaacaagca ggcttggttg cggtgttcta tatgatcatc ggagcgtcaa tgtgggagac
     6601 cattatctac acctcagtgt tgatggctct ttattggggt atttcgtcta atattatgta
     6661 taaaccaacc caagcggtaa caggcggtgc aggcttctca attggtcacc aacaacaaat
     6721 tgcttcttgg attgcagtca aactcgcacc aaaacttggc gataaagacg atactgtaga
     6781 taaaatgaaa ttgcctaact ggctcaatat tttccacgac agtatttctg caaccgcatt
     6841 agtaatgacc atcttttttg gaatcatctt actttcattc ggtttagata acctcaaaga
     6901 acttgctggc aaaaccaact ggtttatgta cattcttgaa atgggcttga aatttgcggt
     6961 ggcaattcaa atcattgtaa ccggcgtacg tatgtttgta gctgagcttt cagaagcctt
     7021 caaaggtatt gctgaacgct taattccaaa ctcagtatta gcgattgact gtgccgctat
     7081 ctacgctttc tcgccaaatg cgatggtatt cggctttatg tggggggcta tcggtcaatt
     7141 tgtggcagta ggcggcttgc ttgcagtagg ctcaccggtg cttattattc cgggctttat
     7201 cccgatgttc ttctctaacg caaccattgg tgtgtttgca aaccaatttg gtggttgaaa
     7261 gcagtaatga aaatctgttt tgtgatgggt attattgaag tgcttggctc agcttgggtt
     7321 attcaaatgc ttgcttcaca aggcacaacc tttaacggtt ggatgggtat ggctgactgg
     7381 gcattgttct tcccaccaat cctacaaggt attatcagca ttccgggctt cttctttgta
     7441 attgtggcag cagcaatggt ttatatgttc ttcgcatcta aacaacttcg tgccgaagaa
     7501 gcagaagcgg cagctcgtgg cattacactt gaacaattag acggctacgg cttagatgaa
     7561 gtaaaagcag aaccaaaagc ggaggcagta gtgcaacaag cggtcgaaaa tgccgaaaat
     7621 tctgcaaacc caatccgcat tctagcggtg tgtggctctg gtcaaggctc atcaatgatg
     7681 atgaaaatga agatcaaagg ctatctcgac aaacgtggta ttccgaatat tatggattcc
     7741 tgtgcgctaa ctgattacaa aggcaaatta agcgaggttg atattattgt ctccagcaaa
     7801 catttagcca acgaaatcga agtaccggaa ggcaaagcgg tattaggtgt acaaaatatg
     7861 ctaaatccaa actcatttgg tgatgaatta gttgagctaa tcaatcagca taatgcaaaa
     7921 taagtaagtt taggggtgaa taatcacccc tatctatgct aaagattgta aaaactcact
     7981 tcatcaaacg aggaattatc caatgaaaaa aatcttatcc accctgttta tgctctccgc
     8041 ggttagcgtt gccgttgcca aagaggtaaa cattaaacta cttggtacct cagacgttca
     8101 cggacgtgtt gtgccgtgga gctacggggc agacgtggaa gacaaatccg gctcattcgc
     8161 ccaaattgca acctatgtca aagaggtgcg tgccaaccac aaaaatgtgt tgctgtttga
     8221 tattggcgat gcgattcaag acaaccaagt cgaagagttc gctaaaacct tagacaaagc
     8281 gaaaaacaat cccgtgccga aagtgctgaa tgcaatgaat tacgacatct tcattctcgg
     8341 caaccacgaa tttaacttcg gaatgccgaa tttagatgcg attttaaaag acattaacgc
     8401 caaaattctc actgccaact tctatcacaa agacggcaaa cgctacgttg agccaactac
     8461 catcattgaa cgtgatggcg taaaacttgg gattatcggc ttaaccaccc caatgtcggc
     8521 tatttttgaa aaagacaccg gtcatttaga cgcaatgaag ttcacttcgc cgattgaaga
     8581 ggcgaaagcc caaattgccg atctcaaagc caaaggcgtg gacgccatta ttgtgctggc
     8641 acatatgggg attgagaacg aaaacaacat tcccgacacc ggcgtggcgg atttagtgaa
     8701 tgcagtggac ggtattgatg taattatcgc cggccatatg cataaagatg tgccgagtga
     8761 aactatcaaa aataccctga ttaccgaacc gcaccgctac ggcacagtgg tttccgaagt
     8821 tgatcttgcc tttgatgtga atgacaaagg cgaagtgaaa ctactttcta aaaccgccaa
     8881 aaccgtaccg gtgaagaaac tcgcaagcga tccggaaatc gaaaaaattt accaaccgta
     8941 tcacaatgaa ctgcgtgcct taaacaacgt caaaatcggt gaaactgcac aagatttaat
     9001 cccacaaggc aaaatcacgg tgtatcgatt gctttctcgc aagacaccgg aatgtcctca
     9061 ttgatccacg atgtgcaaca acattacagc ggtgcagacg tagtgagttt tgcatttgac
     9121 tacgaaaccg tgcgtttaga caaaggcgac atcaagaaaa aagacatcat ctacaactac
     9181 cgctatgccg gtggcgatgt gagcatgtat gagctaaccg gcaaacagct taaacagtat
     9241 atggaatggt cagcggatta cttcgacacc attcaagccg gtgacaaaga ctaccgctac
     9301 aacgctgtgc ggggtaaatc taaatacgtt accttcgacc tattcggcgg cgtaagctac
     9361 aacatcgacc tacgcaatcc aagcggtagc aaaattgtcg atctaaaact ggcaaacggc
     9421 agtctaatca ccgatgacgg caaatacaaa gtgggaatga actcctaccg ctttggtcag
     9481 ctcaccaaaa aaggcggcat ctgggaagga cagtaaatcc ccgtgatttg ggaatccaaa
     9541 gtcgccatgg gggctgaaaa aggcacaatc caaaatatgc tgattgatta catcaccaac
     9601 gtcaaaggcg gaaaaatcga aggcaaatcg cataaccgct gggcgattat cggcttaaat
     9661 taacgaaagg gaaacactat gaacttaaaa caatctctta ttgaaaataa ctctattttg
     9721 ctcaatcaat cagcggcgaa ttgggaagac gcaatcaaaa tcggcactga tttattagtc
     9781 aaagccggta cgattgaacc acgctattac gataatattg taagcaaaat caaagagatg
     9841 gggccttata ttattcttgc accgggcttg gcaatgcctc acgcccgtcc ggaagaaggt
     9901 gtgattaaaa cctctttcgc tttagtgact tttgacaccc cgatttattt tgagggcgaa
     9961 gatgagccgg ttcacgttat gctaaccctt gcagggagtg actctgatca gcatatgcaa
    10021 ggcttggtag aaatcaccca agtgctagat gacccagaga gcgacacagg tgttgattta
    10081 gataaaatcc gccgctgtaa aacggctgaa gaagtgtatg cagtaatcga tcaggcatta
    10141 gcctaatatt ctacaagcgg ttcaaaaaca tcaatatttt gcaaatagag gaatacaatt
    10201 atgtccaaac cattattaca aattgccctt gattctcttt cactggaaaa agcggtggcg
    10261 gatgcaaaac aagcagaaaa ccaagtcgaa attattgaat gcggtactat tcttgctttt
    10321 gctgagggta tgaaagccgt ttccaccttg cgtgcattgc acccaaatca cattattgtg
    10381 tgcgacttaa aaaccaccga tggcggtgca attttagcta aaatggcatt tgaagccggt
    10441 gccgactggc tcacggtttc ggctgcggct caccctgcaa ccaaagcggc ttgcaaaaaa
    10501 gtggcggacg aatttaacgc tgctcacccg gagctaaaag tgaaaaaaga gattcaaatt
    10561 gaaatctacg gcaactggac ggtggaaaaa gatgccaaag agtgggtaga attaggggta
    10621 acccaagcga tttaccaccg ctcccgtgat gcggaactag caggtaaagg ttggacaacg
    10681 gaagatgttg agttaatgaa acagctttct gacttaggca ttgaattatc tatcaccggc
    10741 ggtatcgtac cggaagatat tcacctgttc aaagagatca aaaacgccaa agcctttatt
    10801 gcaggccgtg ctttagtcgg tgaaaaaggc aaacaaactg ccgaagccat tcgtgtggag
    10861 attgataaat actggaacta atccggcatt gaaacccaat aaaaatggcg ttctctcaac
    10921 cggaacgcca ttttttatct cattttttct attcttgcga ggccgatatt ttccacaatc
    10981 tcgctacatt ttcggcagtt gaaatcaggt tctctttgcc tgattttaac accgcttcta
    11041 aattgctgac ctgtcgaata atcggaaata ccgcatcaat gccgtgttgg tacaccacag
    11101 ggtaatcttc tttcaatgag ccaactatcg caatcaccgg cttgccaaac tgcttagcga
    11161 ctttcgccac cccaatcggg gttttaccgg cgatagattg agcgtccatt cgcccttccc
    11221 ctgtcaccac caaatccgca tcggcaagtt tctcagcaag atcggttgcc tcaagaataa
    11281 tttgcacgcc ggaacgtaat gttacgttag gcagtagcag taaaccaccg cccattccac
    11341 cggctgcacc ggcaccggct ttctcggcaa tctcaatgcc taattgggat tttacggttt
    11401 cggcaaaatg caacagagcg tggtcgagcg ttttcaccat ctctgctgtg gctcctttct
    11461 gtggcccaaa aattgccgaa gcccctcgct cgccacacag cggattatct acatcgcaag
    11521 caacctcaaa ttccacttgc tttaagcgtg aatccaaacc gcttaaatca atctgctgaa
    11581 tttggcttaa tgcctcgcca ccaaagccga tttcccgctg ttcggcattt agaaatttcg
    11641 ctcctaaggc ttgtagcatt ccgacaccgc catcgttagt cgcactgccg ccaatgccga
    11701 gaatgatttt ttccacccct aaatctaagg cggctttaat taactgcccc gtgccgtagc
    11761 tggtggtaac cagcggattt cgttgctcca ttggcactaa atgcaagcca cttgccgccg
    11821 ccatttcaat aaaggcggtt ttttgatccc ccgacaagcc aaaaaagccc tccacctcat
    11881 ttgcaagcgg tgcaattacg ctgatttttt gcaaactgcc gtgagtggca tcaaccagcg
    11941 actgtaccga gccttcgcca ccgtctgcca tcggtacgag ctgatactcc gcctcaggga
    12001 aaacacgggc aaaaccctgt tgaattgctt gagccacttc taatgcggtc aaactctctt
    12061 taaatgaatc cggtgcaatc acaattttca tcgtcaatcc ttagagtaaa atcaatgaaa
    12121 gcagataaac cgtgagcata cccaccacac ccataatgaa agttagggta gtttgcgtac
    12181 gatagccttg ttgcggagtc agtttagtaa agtttgttac cacccagtag tagctgtcat
    12241 tggcgtggga aacgcacatt gagccagccg aaatcgccat tacggttaag gctgccgcca
    12301 tttcactggt tagccctaag gcgttcatta atgagtccgg tgcgttgaac attcccataa
    12361 tggaggcggt ggtgataatt gccaccgttg agctgccttg tgcggttttt aaaattgccg
    12421 aaatcaggaa cgggaagaaa atccctgctg tactgataat atgagcgttt tgcttgatgt
    12481 aatccacaaa gccggcttga gtaatcacgt tacctagcac accaccggcg gcggtaataa
    12541 agagaattgg gccgactaat ttcagggtat cattggttaa gctgtcaaat tccgccattt
    12601 taccgcttga aaccagtagg aaaatagcga agatcacccc aattgctaac gcaataatcg
    12661 ggttgccgac aaattgtagg aatacgccga aatcgccact tacaccggca attttggcaa
    12721 tcgagccgat agccattaag ataatcggca taaaaatcgg ggcaaggctt aaaaacccac
    12781 ttggtaattt gccgtattgt ttgatcagat cttcatagct ttggctgatt acttgatccg
    12841 cttgagcttc ttccatcacg ctgacttgtt tgccaacata tttggcaaag aagtaactgg
    12901 caatcaacac aggaatcgaa accacaaagc ccattccgat cacaagcagg agattatggc
    12961 ttaaaccaac agcaccggct gctgcaatcg ggcccggtgt cggaggaata aagacgtggg
    13021 aggcatataa accggcactt aacgccaccg ccattcccac cggattggca agaatttttt
    13081 tgcgaatagc ttcacgaatc gggtttaaca ccacaaagcc gctatcacag aacactggga
    13141 taccaaccac ccagcccata atcaacattg ccagttcagg gcgtttttgc cccaccactt
    13201 ttaccaccat atcggctaat ttgagggctg caccggtttt ttctaaaatc gtgccgataa
    13261 tcgccccaaa aataatcacg ataccgatac ttttgaaggt attactaaaa ccttcgccca
    13321 caatagttgg aattttggca agcggtaaac ctgccactaa tgcaagggct atcgaaatcg
    13381 acattaaggc taagaacggg tgaactttcc acttcgcaat catataaatc attatgatga
    13441 tagcgaccag aaagatcaga ataagtgcaa tacctgacat aataagtctc ctcattgtag
    13501 aaaaatgaac ttattattcg gtatttattg ctaaaaagat ttagataaat ttgcaaaaaa
    13561 tttgttaaaa acgaccgctt gtttgtatat ttttacaatt tttacgacag aaccgctcca
    13621 agatacagca caaatttttg ctctatgtta ttgaaagata aaccagttat ctgctcaatc
    13681 ttctctaaac ggtaacgtag cgtatttggg tgaataaaca attttttggc agcgtgatcc
    13741 agatcacaat ttgcgaaaaa atagtaacgc agacttttca gtaacacctg ttttttatcg
    13801 gaaaccagta aagcttggaa cggcgagagc aaggttttcg cttcccaaga gtggctgaaa
    13861 ctgtgcaaga gggccggcag tttaaaatct tcaaaatgaa taatctgctt acggctttgg
    13921 atctgctcgg cataattgag ggtatggcag gcggtttggt aggaaatatg ggtttgcgat
    13981 aaattttcga ccgccagccc aaccaccgtt ttgaaggaaa tatccggatc gttaagtttg
    14041 tttagtgcat tgcggattaa cgatttttcg ttggataaat gattcagcaa ggcaatttta
    14101 tctaacccaa tcacggcggt gagtaggctc gggaaataat gttcgaaaaa atcaatcagg
    14161 cgttggagtt tttccgcagt agagccgtga attttaatca ccatcaccga cagcggtttt
    14221 gcaaaatctt gcccgaaaaa gaccgcttgt tgctggattt ctgctgtgct taggctacct
    14281 ttgagcaagg cccgcacaaa ctcttcccga tagcggcgtt gccaacgttc ctgttccaac
    14341 tcaaacgctt gctgcataat cagctccgcc gccattttca ccaattgagc atattgccga
    14401 acttgttgtg gctgccctga aatccccact acaccaatgg ttgagcctaa atagtgaagc
    14461 ggtaagttaa tgcccgcttt cacttcatcg caccactgtt ttgcaagatt ttcatcaatt
    14521 tcgaccgctt gatcgttgcg taacgcaatc accgctcctg tgtgtcgttg cccgatacga
    14581 ccgacatcgc cggaagcaat aatcacccct tcactatcca tcacattgac ggagttggga
    14641 ataatctgca tcgtgcgctt gacgatgcgg tcggcaatcg cttggttgag tttcattata
    14701 tgattcctac tcattcccaa tactatgaaa aatccctctt tcgagggatt tgcaaattat
    14761 ctacgcttcc acttcctgag aatagatcat agcgtgaagt tattttgcca cagtcgcttg
    14821 aattaatttt tccgctttgg caaggttgtt ttctacatca gcataatcgg tattagcgtg
    14881 agccaccact tgatcggcac gatttaacat tacttcgacc ttgcccttgt tcggttcggt
    14941 aattaacact tttaacggta attgcaacgc taacatcgga tctttcacca ttaatggcgt
    15001 accggctttc ggtgtgccgt aaaccaatac ggttgccggt tgcatttcta agccggcttg
    15061 ttttgccgcc gcttggtgat cgatttcggc aaaaatcgtc attccttttt cttttaaggt
    15121 gttggttaaa gttgtgactg tagtttggaa atcaaattta ctttcaaata ccaccggttt
    15181 gacatcggca atatctgcca ttgccacgct gctcattgct aatgcagaaa ctgcgactgc
    15241 ttttaagttc atttcttttc tcctcaaata gattaaataa tcccttgttc ttccaacttg
    15301 cctaagaaat aaggcatctg tttacgccaa gtcggccagt catggtcaat atcaaagccc
    15361 cagtagtcaa accaagcatc gatccctttt gcgttaaaag cctcttgtaa tcggcgggtg
    15421 tcctcaatat gctgttcttc ccacgcacct tgtccgactg caatcacata gtgattttgg
    15481 cggtagcggt ctaagaacca atcgtcattt tgaccccata cataatcaat cggcgagtta
    15541 aaataaaccg ctttatcgcc ataaaactca ccactgaaaa agcgagcgtc ataaacaccg
    15601 cttaaggcga tggcaatatc aaacagatcc ggatgacgta aggcaaagtt taccgtatgg
    15661 aaagccccca tactacaacc ggtggcaatc atcgtgccgt tccagtgcga ttcatggcgg
    15721 attaacggca ctagctcgtg aataatataa cggtcgtaag cattatgagc taatgccata
    15781 tcgtgtccgg atttatgttt agataaccaa gattccgcat cgtaagaatc aggcgtgtaa
    15841 aatttgatta agccacgttc aataaaggaa cggcaagcct caatcattcc aaaattacca
    15901 tactcgtttt cattgccgcc ggaggaaggg aaaacaataa ccggtttacc tgcgtgtccg
    15961 taaacgttga acgacatttc acgacctaat tctccactcc agtggcttct tttttcaaaa
    16021 tgcatacttc acctcttact gtttagcttg tgcaaattgt gcaatttcac gcatttgctc
    16081 cattgtttcg gtacgggcta aaatgccttg ttcgcccatc actttggcaa atacgcccgg
    16141 tacgctttgc acgctgataa tattgccggc aaatttctca caaacagctt caatcgaatt
    16201 cacataattt tggtttgcct tgcgggaaat ataaaccacg ttccaagggt gctgaatatc
    16261 ggcatagaat ttgttttctt tcacaatatt ggcaaattca cggaacacat cgaaatcgtt
    16321 ggcgtagttc cacatatcaa tagttaagcc acccggcgga cggcagttga tttcgagtgg
    16381 taacaactct tcttttttgc tccctttaac acagaagaac tcaaaatgga aaaaacgctc
    16441 ttttacgtta aaggctttca cacattgttt gcccatttcc actaattttg gcgaaatttc
    16501 acatgggaca tagtagaaca tatcgccatc ttttgaaacg gtatctaata ccgcttctga
    16561 atattcaagg ctggagtaga aaacgatatt gccctcttta tcggttaaac cgtcaaaggt
    16621 aataatatcg ccgtcaataa actcttccat aatatattca accgttggcg ttttataacc
    16681 gaagaaagct tctaactctt ccgcagtttt gattttgtaa gtatcgcttg ccccaacccc
    16741 tgaatttggc ttgataatca ccgggaattt aagctgtttt gctaatttgc aggcatcttc
    16801 atcatcttta aatactcgcc cttgtgccac tttcaagccg gttttgcgga atacctcttt
    16861 catttgggct ttggttttaa ttgagagcat atcatcgctc ttataaccaa acacattgaa
    16921 atcggtacgc aatttcgcat ctaactccag ccaatattca ttgtgcgatt caatgcggtc
    16981 aattcgcccg tatttatggg caaagtacgc gaccgcacga taaacttgtt cgtaatcttc
    17041 catattatct acacgataat attcagtcag ggaatgacgc agattttcgc tcaatgattc
    17101 ataaggggca tctgcaattc ccaatgtatt aaaaccggct tcacgcaaac gaatggcaaa
    17161 ggtttcaaag ttcgttggga agtgcgggga aatcatcaca aaattaagtg gtctagacat
    17221 agttcttcct ttataaagtt tgcatattat taaaatgatt agaatagatt ttaggctaaa
    17281 tttattaatt ttcaaaatgt attcacacaa gcaataattt ttacaataaa ttaactttta
    17341 taaaaactca atttaacatc agaataaatc gtataaattt agtcaggtat ttttacaaaa
    17401 aaaccatatt tatgatttac attaatattt ccatctatta tcctaaatat gttgtcagta
    17461 tttatactga taaacacata acaaaattca tttatattaa tggagattca acatggctga
    17521 acaaattgtt ccttcaaatg aagctgtttt aaaagcaggc cacgatactt tagttatttc
    17581 aacaactgat ttacaaggtg ttattactta cgctaacgac cttttctgca aattaaccgg
    17641 ttttacccgt gaagaattaa ttggacaacc gcataatatc gtacgccacc cagctgtacc
    17701 aaaagaagtt tataaagata tgtgggatac aatccaagcc ggtaaaatct ggacaggtat
    17761 cgtaccaaac tgcggtaaag gcggtgtagt ttatgtggtt gataccaccg ttcaaccaat
    17821 gtttgatgaa acaggcaaaa ttacaggcta tatcagcgtt cgccgtgtaa ttaatgattt
    17881 aatgaccaac tttgaagcag tagaatcagc aaaagaaatc tttgatgact tctacggtga
    17941 ataattaaaa aacaatgatt gcgtgtaaaa gatgatctct tttacacgca attttttatt
    18001 ttcgaggagt ttgtatgtct ctcattcccc atatccacat tgcttatggt tcggagtctg
    18061 gtaacgcaga aaagcttgcc caacaattag ctatccaacc ctttttactt ccctactcac
    18121 ccacactttc tacattaaac gaaaccgatt taagccaatt atctgccgag agtttattgc
    18181 ttattgtaac cagctccttt ggtgatggcg aaccacctga aaacgccgat gaatttgcag
    18241 aaaaattgga aaatttgacc gcttgtaatc tgcaatatgc gatattcggg ctaggtgata
    18301 ttacttatga taaattctgt ggctatagca aacaactaga ttcattatta caacaaaaac
    18361 aagctaaagc tgtgattgca agggttgatg ccgatttaaa ctatcaagaa atttttaaac
    18421 aatggctacc cctagtggag caggcattac attcaacaaa caaagaaacg atccaacatt
    18481 ccctttctgt tcaagcttac ggcgaggaaa tggcatacca agcagaaatg ttggaaatta
    18541 agcatttggc aaactcaagc cctgcggtgt atcacattcg tttatcatta aaagagagcg
    18601 gtattttcta tcaagcaggg gatttaattt atgtcaaagt gcagcaacca gacagactat
    18661 taaatgaata tgcagaatgg tttaacgatc aaaatgcagt tgcattacta cgccataaag
    18721 aactccgact actaagtaaa aatgtactgc gtgatattca aaaaatttgc ggcagtaatg
    18781 aactcaaaga tttaactaaa atttccaata aaaaagcatt ggaacaatat gtctatgggc
    18841 gggatttatt agacgtatta cgcgattttg acccaaacaa aagcgtgaca ttagccgatt
    18901 tagagccaat tttatctaac ttagccgcac gagcctattc aattagctct tgcggtaaaa
    18961 ctcaccctga ttatgttgat ttatgtatcc gccacgtttc gtatcattta aacggtagag
    19021 attatcaagg gactgcaagt gggtatttat ccgaattaca agcgggcgat tttgtctcga
    19081 tttttgcaaa tgccaatccg aatttccgtt tacccgaaac attaaccgct ccggtggtga
    19141 tgattggctc aggtactggc attgctccgc atatcgcatt tttacaagcg ttagagcagc
    19201 aatatcaggg ggttgaaagc tatttattct tcggcgagcg ttatcgtgat aaagattttc
    19261 tgtatcaaac ggaactggaa aattacttag caaaaggcac cttaactcag ctgtttactg
    19321 ctttttcacg tgatcaggca gagaagtttt atgtgcaaga tgccctatcg caacaagcag
    19381 agttagtttg gcatttaatt cagcaagggg cttatttcta tatttgtggt agcaaagcaa
    19441 tgagtaaagc aatagatgcg gaactcatca aaattgcaga tcaaattgga ggcgagcctt
    19501 atattgatga tttcaataat attattgcaa aattggtcgc agaaggccga ttaatgcgag
    19561 atgtatatta gcaaatgaaa aaaactaaaa acccagcaaa agctgggttt ttatttaatc
    19621 atagttcatt aagcaacaac tgctttctca actacacgaa ctaaagtcca agatttagtt
    19681 tttgaaatag gacgacattc cttaatttca acaacatcac ctaatttagc ttcgttgttt
    19741 tcatcgtgta catgaagttt agtagtacga cggataaact taccgtataa cggatgttta
    19801 actgtacgtt cgatagcaac aacaaatgat ttatccattt tgtcgctaac cactttacct
    19861 tgtactgtac gaattttatc agtcattatt cacccgcctt ttcggttaat acagttttaa
    19921 cttgtgcaat actacgacgc acttgtttta actggtgggt ttgttgaagc tgaccggtgg
    19981 ctgcttgcat acgcaatttg aattgttcac ctaaaaggtt cactaactca ccattcagct
    20041 cttcaacact tttattacgt agttcttgag ctttcattac atcaccgtct tagttacaaa
    20101 tgtggttttg aacggaagtt tagctgccgc taatgcaaac gcattgcgtg caacttcttc
    20161 agaaacacca tccatttcat agagaacttt acccggttgg attaaggcta cccagtactc
    20221 aacgttacct ttacctttac ccatacggac ttctaatggt ttttcagtaa ttggtttgtc
    20281 tgggaacaca cggatccaga ttttaccttg acgtttaacc gcacggctca tcgcacgacg
    20341 agcagcttca atttgacgag ctgttaaacg accacggcca atagctttta aaccaaatga
    20401 accgaagctc acttctgtgc cacccgcgat accacggtta cggcctttgt gtactttacg
    20461 gaattttgtg cgttttggtt gtaacattca gtgattctcc ttatttacga cctttgccac
    20521 gtggcgcctt tttaggctgt gccgctggtt ctttttcaga ttcaatcact gcagccattc
    20581 caccaagaat ttcacctttg aagatccaca cttttacgcc gattacgccg taagtagtgt
    20641 gagcttcagc agtactataa tcgatgtctg cacgaagcgt atgtagaggt acacgacctt
    20701 cacgatacca ttctgagcga gcaatttctg caccacctaa acgaccgcta acttcaactt
    20761 tgataccttt agcacctaat ttcatcgcgt tttgaactgc ttttttcatt gcacgacgga
    20821 acatcacacg acgttcaagt tgagaagcga tgctatctgc gactaatttc gcatctagct
    20881 ctggtttttt cacttcagcg atgttgattt gagctggaac gccagcgatt ttcgcaactg
    20941 cgttgcgtaa tttttcaaca tcttcacctt ttttaccgat aacgataccc gggcgagctg
    21001 tgtgaatagt tacacgaatg ctttttgcag gacgctcaat agtgatgcgt gatacagaag
    21061 cgttcgctaa ctctttgttt aaaaatttac gtactttgaa gtcgccgtct aagttatcag
    21121 cgaaatcttg tgtattcgcg aaccaagtag agttccaagg tttaacaata cctaggcgaa
    21181 taccattagg atgtactttc tgacccattt gttattctcc tacttaatta acgatctgac
    21241 acaaccacag tgatgtggct tgtacgtttt aaaatacgat ctgcacgacc ttttgcacgt
    21301 ggcattacac gtttcatgct tggaccttca tcaacgaaga ttttagcaac tttaagatca
    21361 tcgacatctg caccgtcatt atgctctgcg tttgcaatag ctgactctaa tactttctta
    21421 acaagcgtcg ctgctttttt gttagtgaaa gttaagattt ctaatgctac agaaactttt
    21481 ttaccgcgaa ttaaatcagc aactaagcga gctttttggg cagaagtacg agcgtaacga
    21541 tgtttagcaa tagtttccat cttttacctc ttatttctta gctttcttat ctgcggcgtg
    21601 accgcggtat gtacgagtcg gtgcaaattc acctaattta tgaccgatca tttcgtcgga
    21661 aacataaaca ggaacgtgct gacgaccatt atggactgcg atggtcaaac caatcattga
    21721 tggaatgatc attgaacgac gggaccaagt tttaattggt tttttatccc cgctttccac
    21781 cgccttctct accttcttca acaagtgtag gtcaaggaaa ggacctttct tgagggaacg
    21841 tggcattggt gtttaccttt tagttaatga attaaattat ttgccacgac gacgtacgat
    21901 gaatttatca gtacgtttgt tgtgacgagt tttcttacct ttggtttgaa cgccccaagg
    21961 tgtaacaggg tgtttaccga agttacgacc ttcaccacca ccgtgtgggt gatctaccgg
    22021 gttcattgca gtaccacgaa cagttggacg aatacctctc caacggtttg cacctgcttt
    22081 accaagaacg cgaagcatat gctctgagtt acctacttca ccaatggttg cagtacactc
    22141 agctaatact ttacgcattt cgcctgaacg aagacgtaaa gttacatagt taccttcacg
    22201 ggcaatgatt tgaacgtaag caccagcaga acgtgcaatt tgaccacctt taccaggttt
    22261 taattcaacg ttgtgtacag ttgtacctac tgggatgtta cgcattggta atgcgttacc
    22321 tactttaatc ggagctgaag cacccgcttg aatttgatca ccagcagtta aaccttttgg
    22381 tgctaaaata tagcgacgtt caccatcttt ataaagtact aatgcgatat tcgcagaacg
    22441 atttggatcg tattctaaac gctcaacaac acccggaatg tctaatttat tacgtttaaa
    22501 atcaattaaa cggtagtgtt gtttgtgacc gccaccaatg tgacgagttg tgatacgacc
    22561 taagttgtta cgaccaccag ttttagattt agtatctaaa agtgctgcaa aaggtttacc
    22621 tttgtgaagc tcagcgttta caactttaac aacgtgacga cgaccagcgg aggtcggctt
    22681 acatttaacg atagccattt attttatctc ctctgattac tctgccgcac cttcaacgaa
    22741 gtcaagcgat tgaccttctt gaagagttac ataagctttt ttccagtcac tacgacgacc
    22801 aactttagca ccacggcgtt tagttttacc tttaactact acggtacgaa cagaatcaac
    22861 ttttacttca aacagttctt caactgcatt tgcaatctca actttgtttg catctaatgc
    22921 aactttgaat acgattgtat tgcctttttc tgcgttgttt gtcgcttttt cagagatatg
    22981 tggtgcttta agcacgttta gcaaacgttc ttgttgaatc atgctaacat ctcctcaatt
    23041 tgttttactg catctacggt tacaaccact ttatcgaaag caattaagct aactgggtca
    23101 ataccttgca catcacgcac atcaacttta tataagttgc gtgctgctaa aaataaattc
    23161 tcatctacgt ttgcagtaat gataagagcg tcatttaacg ccatatcttt taatttttgt
    23221 gctaatactt tagtttttgg tgcatcgatt tcgaatttct caacaaccac taaacggtct
    23281 tgacgaacta attcagaaag aatgcttttg attgcaccac ggtacatttt tttgttcact
    23341 ttttggctgt gatcttgtgg tttcgcagca aaagttacac cacccgaacg ccaaattggt
    23401 gatttaatat caccagaacg cgcacggcct gtaccttttt gacgccaagg ttttttacct
    23461 gaaccagaca cttcagcacg agttttttga gcacgagatc cttgacgagc acctgctgca
    23521 taagcaacaa ctacttggtg gattaatgct tcattgaact cacgtccgaa ggtagtttca
    23581 gaaacagtta gtgcgtttgc acctacaact tgtaattcca tctctatctc ctagacttat
    23641 gctttaactg ccggttttac gataacatca ccattagttg caccaggaac ggcaccttta
    23701 actaataata atttacgctc aacatctaca cgaacaactt caagtgattg aacggttaca
    23761 cgctctgcac ctaagtgtcc tgccattttt ttacctttaa acacacgacc tggagtttgg
    23821 ttttgaccaa tagaaccaag tacacgatgt gataaagagt taccgtgagt tgcatcttga
    23881 gtacggaagt tccaacgttt aacaccacct tggaagcctt taccttttga tgtaccagta
    23941 acatcgactt ttttaacatc agtaaagatg tcaacattga tttcttgacc taaagtgaat
    24001 tcttcacctt cagtagtacg aaattcccat aaaccgcgtc cagcttcaac acctgctttc
    24061 acgaaatgac ctgcttctgg tttagttaca cggctagctt ttttagagcc agtagtaact
    24121 tgaattgcag agtagccatc gttttcaagg gttttaactt gagtaacacg gttggcttcg
    24181 atttcgataa cggtaactgg aatagacacg ccgtcttcag tgaaaacgcg ggtcatacca
    24241 actttacgac cgattaaacc aatcattgta ataacctctt aaaattccca attaacctag
    24301 actaatctga acgtcaacgc cagcagctag atctaaacgc attaatgcat caacagtttt
    24361 ttctgttggt tcaacaatat caactaaacg tttgtgagta cggatttcat attggtcacg
    24421 tgcatcttta ttcacgtgtg gagaaatcaa tacagtgaaa cgttctttac gagttggtaa
    24481 aggaattgga ccacgaactt gtgcaccagt acgtttagct gtttctacga tctccgcagt
    24541 agattgatca attaaacgat gatcaaatgc ttttaagcgg atacggattc tttggttctg
    24601 cattagacca gagctccaat taaaaaattt agctaataaa aaaaccaaac taccacttaa
    24661 caaattagtt aagggagctc agttaaacct atatagttcc aaaattagga acattgtata
    24721 cactacaata tctgtagtgc catttgataa atgattacat caaatgtctt tagataccta
    24781 aagctttgct atcatactcg tttcatttcc cttatgcaag taatttttgt tttttatatt
    24841 gacaataatt tgattcgata aaacttctaa agatacacaa tatttccaat acttcatttg
    24901 aagtggctca aataaattgt atttatgcat ttttttattc aaacttttcg agccacttca
    24961 caaatctaaa aaatcctagt tgaaatttaa tccataagag tatttattat taaataagtt
    25021 tcatgacctt acatcggagt acatatatgc aaacaatcaa tgaaattaaa gaagaaatct
    25081 accctatatt tgagcctatc attggaacaa ttcggctaaa aacctacttt tcctactacg
    25141 cagtttttaa ggatggattg atgattgcat tgtatcaaaa tggagcaact tatttacgta
    25201 ttgcgaaaaa agatattgaa tacattcgtc agtatcctga aacctgtatc ttatgcgatg
    25261 aaaaaattgg tatacaaagt agaaaatttc attatattcc gcaagttctc atagatgata
    25321 caccaaaatt tagctcgcta ctctttggaa ttatagagga actccgaacg gagaagtaca
    25381 aaataagcca aaagcgagct acacaaatcc gaaacctacc aaatatgaat ttaaagttag
    25441 aaagaatgtt gaaaaaaata gggattcatt ccattcatga ttttactcaa gaagggtata
    25501 tgtcaacttt tatcaaactg attctccagg gctttgatgt taccgaagac ttactgttta
    25561 agctaaacgg agctttaaat caccaatata tttacacctt cacagatcaa caaaaaaggg
    25621 aactaatgca agaagcaaat caagcgctat ataattcagg cctaagaaaa agatttaacg
    25681 ccctgtagtt aaataatgtg atccaaatca taaaatataa aaaaaccaat aaggaattgc
    25741 aattcgcagt tcctttcttt ttcttttata tgctagtatc accttatacc tctaaattat
    25801 tagtgcctac tgattttcaa ttaatccaac aatttaggag agcgaatcat ggaattccgt
    25861 atcgaaaaag acacaatggg cgaagtgcaa gttcctgccg ataaatactg ggcggcgcaa
    25921 accgagcgtt cacgcaataa cttcaaaatc gggccggagg catcaatgcc ggcggaaatt
    25981 atcgaggcgt ttggctatct gaaaaaagcg gctgcctatg ctaatcacga tttaggcgta
    26041 ctagcggtcg aaaaacggga tttaattgca aaagcctgtg atgaaatttt ggcgaacaaa
    26101 ctcgatgatc aattcccgct ggtgatttgg caaaccggct cgggtacgca gtcaaatatg
    26161 aacgtgaatg aagtcgtggc aaaccgtgca cacgtgttaa acggcggtaa gctgggtgaa
    26221 aaatcgatta ttcacccgaa tgatgatgtg aataaatccc aatcttctaa cgacacctac
    26281 ccaactgcaa tgcacatcgc tgcttacaaa aaagtggtgg aacacaccct gccttgtgtg
    26341 aaacgtctgc aaaaaacctt agcggaaaaa gccgaagcct ttaaagatgt ggtcaaaata
    26401 gggcgtactc acttaatgga tgctaccccg ctcacgctcg gtcaagagtt ctctgcctat
    26461 gcagcacagt tagcattcgg catcaaagca ttggaaaaca ccctgccaca cctgcgtgag
    26521 ctggcactcg gcggcacggc agtcggcacc ggcttgaata ccccgcaagg ttacgatgtg
    26581 aaagtggcgg aatacattgc gaaattcacc ggtttaccgt tcgttaccgc ccaaaacaaa
    26641 tttgaggcat tagcgactca cgatgcgatt gtcgaaaccc acggtgcatt aaaacagctt
    26701 gcggtgtcgc tctacaaaat tgccaacgat gtccgtttat tagcctccgg tcctcgttca
    26761 ggcattggcg aaattctgat tccggaaaac gagccgggtt catcaattat gccgggtaaa
    26821 gtaaacccaa cccaatgcga agcaatgaca atggtatgtg cccaagtatt aggcaacgat
    26881 gtaaccatct cttttgccgg cacgcaaggg catttccaac tgaatgtcta taaaccggtg
    26941 atggcgtaca acttcctgca atcggctcaa ctgctaggcg atgcgtgcgt gtcgtttgac
    27001 gaacactgtg cagtcggcat tgaaccgaac taccctcgca ttaaacaaca gctcgaccaa
    27061 tcgctgatgt tagtgaccgc attaaatact cacattggtt atgaaaatgc agcgaaaatc
    27121 gccaaaactg cccacaaaaa cggcacaaca ctgaaagaag aggcgattaa cttaggctta
    27181 gtcacggcag aacagttcga tgaatgggtt cgtccggaag atatggttgg cagcttgaaa
    27241 taagcccaac ccttctcttg taatctgatt tacacctcaa tctaccggta agattgaggt
    27301 gtaatttttt atagcgaggg tactgatttt gttttagaat gaagcatcat tttttaaacg
    27361 gaaaaaccta tgaaaaaact cattttaatc gcattagtta ctactttagg cttaagtgct
    27421 tgtggggtca aaggcccact ctacttccct gagcaagccc ctaaacatga acaaaaataa
    27481 gcattgcaag cggtctgttt tggctaaaat tttgcaaatt tttgtgaaaa aatgaccgct
    27541 tgttgcctct tttctttcaa aatcccaaac acaacggact ttcaatgaac cattttgaat
    27601 ataaaaacaa ccaacttttt gctgaagaag tcgctattgc cgatattatc gagcaatacg
    27661 gcacgccagc ttatgtttac tccagatcta cccttgaacg ccacttgcac gcattcaata
    27721 atgcgtttgg ctcgcaccca catttagttt gctttgcagt caaatccaac tctaacattg
    27781 ccttgttaaa tgtaatggct cgtctcggtt caggctttga tattgtctct caaggcgagt
    27841 tggagcgtgt cttagctgcc ggcggcgaag cctctaaagt tgtcttttcc ggtgtggcaa
    27901 aatcgcacag cgaaatcaaa cgagcattgg aagtcgggat tcgttgcttt aacattgaat
    27961 cggtttccga acttcaccgc attaaccaag ttgccggtga gctaggcaaa atcgcaccga
    28021 tttcactgcg ggtaaacccg gatgtggacg cccacactca cccatacatt tccaccggtt
    28081 taaaagaaaa caaattcggc gtgagcgtta ctcaagcccg tgaagtgtat cgcttagcca
    28141 aaaccttacc gaatgtgaaa attaccggta tggattgtca catcggttcg caattaaccg
    28201 aattgcaacc ttttttagat gcaacggatc gcttaatcat cttaatggag caactgcgtg
    28261 aagacggcat tgagctacac cacttagact taggtggcgg tttaggcgtg ccttacaacg
    28321 gcgaagagcc tccgcatccg accgaatatg ccaaagcctt gctggaaaag ctcaaaggct
    28381 atgaaaactt agaaatcatt attgaaccgg ggcgtgccat taccgcaaat gccggtattt
    28441 tagtgacgaa ggtggaatac ttgaaacaaa atgaagatcg taattttgcg attgtcgata
    28501 ccggtatgaa cgatatgatt cgcccggctc tgtatgaagc ctatatgcaa attacggaag
    28561 tggataaaac cttaaaccgt gacaaagctg tttacgatgt ggtcggtccg atttgcgaaa
    28621 cctcggattt cttaggcaaa caaagagaat tggcaatttc tgaaggggat ttaatcgcta
    28681 ttcgttctgc cggtgcttat ggggcggcga tgtcttcaac ctacaactct cgcccacaag
    28741 cggcagaaat tatggttgat ggcaaccaag cccatttaat taaagcccga gccacttttg
    28801 ctgatttatg gcgtttagaa aaattattac cttaatgagg aagaaaagat gaaattatat
    28861 ggattaaccg gtgcttgttc atttgtacca cacgttgccc tagagtgggt gaaattgcgt
    28921 gccaatcaag attatgcgtt tcaagctgtt agccgtgaat ttattaaatc ggcggaatat
    28981 ctggctttaa accctcgtgg taacgtacca ttgttggttg atggcgactt ggccctcacg
    29041 caaaaccaag ctatcgtcca ctatttagac gagctttatc cggaagctaa attattcggc
    29101 agcaaaaccg ttcgtgataa agccaaagcg gctcgttggt tagctttttt caattcagat
    29161 gtgcataaat cattcgtgcc actgttccgc ttgccaagtt atgcggaagg caatgaaacg
    29221 ctcaccaaaa ctattcgcca acaatcggct gagcaaattt tagaacaact ggcttttgcg
    29281 aatgctcatt tagaaaatca tattttcttt ggcgaagaaa tttcggttgc cgatgcgtat
    29341 ctctacatta tgctcaactg gtgtcgccta ctagggttgg atttctctca tctttcccaa
    29401 ctttctgcct ttatgcaacg agtggaggca gaccaaggcg tagataatgt gcgtgagcaa
    29461 gaaggcttaa aaggttaatt caccattgtt catttaagac ggtagatttt atgttaaaat
    29521 ctaccgtctt ttatttttag gaatcaccat gctaaatcag gtttcaaaca gcttgcttgc
    29581 tccaagtggg cttgaattat cgcacttaac tcaagtgtta gatcacttct ccggccgtca
    29641 tattgattat gccgatctct acttccaact cagccaagac gaaagctgga gcttggaaga
    29701 cagcatcatc aaagaaggcg gtttttatat cgaccgaggg ttcggtgtgc gagccatatc
    29761 aggcgaaaaa accggctttg cctacgccga tcaaatcacg ttaaaacagc ttgagcaatg
    29821 tgccgctgcc gcacgttcaa tcagcccaac aagcggtcaa ttatcggtaa aaaatttcaa
    29881 aaaaacgacc gcttgttctc gctacccggc aatcaacccg ctagagacaa tcagccgtga
    29941 acaaaaggtc gaattgctcc atttggtgga taaaaccgct cgaatggcag atccgagagt
    30001 gattcaagtg aatgcgaatc tctcagcggt atatgaagaa atgttagtgg cagcgactga
    30061 cggcacactg gctgcggata ttcgcccgtt agtgcgttta tctgtgtcgg tactggtgga
    30121 acaaaatggc aagcgtgaac gaggttcagc aggtgccggt ggtcgctttg gtttagattg
    30181 gttcttagca ccaagcgaaa ccggctttag ccgtgcggaa tcgcttacca aagaagccgt
    30241 tcgccaagcc ctcgtcaatc tcacagccat tcctgcaccg gcaggcacga tgccgattgt
    30301 tctcggtgct ggctggccgg gcattttgct ccacgaagca gtcggacacg gcttggaggg
    30361 tgattttaac cgtaaggaat cttcgctctt cagtggtaaa atcgggcaaa tggtcacctc
    30421 tgagctttgc accattgttg atgacggtac ggtgccgaat atgcgaggct caatcacggt
    30481 agatgacgaa ggcgtgcctt cgcaacgtaa tgcgttaatt gaaaacggca ttctaaaagg
    30541 ctatatgcaa gacaaactca atgcccgcct gatgggagtt gccccaaccg gcaacggcag
    30601 acgggaatct tacgctcatt tgccgatgcc acgaatgacc aacacctacc tcaccgaagg
    30661 caatcacgaa tttgaagaga tgattgaatc ggtcgaatac ggtttatatg ctcctcactt
    30721 cagtggtgga caagtggata ttacctccgg caaatttgtg ttctccaccg ccgaagccta
    30781 cttaatcgaa aaaggcaaaa tcaccaaacc tgttaccggt gcaaccctga tcggcagcgg
    30841 catagaagca atgcaacaag tttcaatggt cggcaaaaaa atggaactcg accccggcat
    30901 cggcacttgt ggcaaagaag ggcaatccgt accagtcggc gtagggcagc ctacagtgaa
    30961 gttggataaa attacggtgg ggggaagagg gtaatctctc cccgtttgca aaatataaag
    31021 aaaatatcac cgcttgtagc gataaaagga aaacaaaatg gcttgggtac aaattcgttt
    31081 aaacagtaca gataaacagg ctgaggaaat cagcgatttt ttaaccgaga ttggagcggt
    31141 gtcagtgact tttatggata gtcaagatac cccaattttt gaacctcttc cgggggaaac
    31201 ccgtctttgg ggaaataccg atgtggttgg gcttttcgat gcggaaactg atatgaatct
    31261 tatcgtagaa gcactaaaaa ccagcaaatt ggtggacgaa aactttgctc acaaaatcga
    31321 gcaaatcgaa gacaaagact gggaacggga gtggatggat aatttccacc caatgcagtt
    31381 tggcaaacgg ttatggatct gcccaagctg gcgcgaagta ccagacccga atgcagtgaa
    31441 tgttatgtta gaccctggtt tagcatttgg caccggcaca cacccaacga cctcgctttg
    31501 cttacaatgg ttagatagct tagatttaga aggcaaaacg gttatcgact tcggctgtgg
    31561 ctccggtatt ttagctatcg ctgcacttaa actcggtgca aaacaagcca tcgggattga
    31621 tatcgaccca caggcgattt tagctagtca aaacaatgct gaggcgaacg gcgtagcaga
    31681 aaaactacaa ctattcttag ccaaagatca gccaagagag ttacaagccg atgtcgttgt
    31741 tgcgaatatt ctagccggcc cactcaaaga gttagcaccg aatatcatca cgctggttaa
    31801 accaaacggc aatttaggct tatccggcat tcttgctact caggctgaaa gtgtgtgtga
    31861 ggcttatagc gaagccttta cgctagaccc tgttgtagaa aaagaagaat ggtgttgtat
    31921 tacaggccgc aaaatctaat ataagaagcc ctgttttaaa cagggcttac tttttgataa
    31981 attctactac tctttacctg tcttgggaat cgttctccct aaattaatta acacaagcgg
    32041 tagaaataac aggaaaattt acaaattaca taaatgaaaa aagccgactg aaatcagtcg
    32101 gctttctaaa taatggcagg ggcggagagg ctcgaactcc caacacccgg ttttggagac
    32161 cggtgctcta ccaattgaac tacgccccta ttgataggta ttcattttgc acttataggg
    32221 gcgaattact attcgccccc tgcattaaag attattggcg gaacggacgg gactcgaacc
    32281 cgcgaccccc tgcgtgacag gcaggtattc taaccagctg aactaccgct cctaaattaa
    32341 tagattgata atgccctact ctcacatggc gaatcaccac actaccatcg gcgttactgc
    32401 gttttacttc tgagttcgga atggagtcag gtagagctac agcaccctgg ttatcaatca
    32461 aattctttca tttagtcagt ttactaaaat atagtttata ctttctaata
//
LOCUS       DANJWC010000009        32472 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.009, whole genome shotgun sequence.
ACCESSION   DANJWC010000009 DANJWC010000000
VERSION     DANJWC010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 32472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32472
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.009"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..523
                     /locus_tag="PWX02_000281"
     CDS             <1..523
                     /locus_tag="PWX02_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728335.1"
                     /translation="NQDYIKNRKGMDDLKSINTDNDNMPVSLTFYDVLKYINWFNQKH
                     NANVRLLNFDEYKFISPFEQTDRNEWKHEDIEFIYDEKISTEPPPYMEEEKFQKIIMR
                     FSKDIKIMMHNHINFIESDRFAEWILEKACVRSKTLTSFYGDKSVIRAVPPLDCSGKY
                     KYTKIGFRLCYDL"
     gene            508..732
                     /locus_tag="PWX02_000282"
     CDS             508..732
                     /locus_tag="PWX02_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDL1728336.1"
                     /translation="MLRFVGGYMKKYSNDSLINSIVISKLKAGWKIRKGKKHNILIAP
                     NSRRLAIPSTPSDINAYKNFRSKLNHLIAF"
     gene            888..1616
                     /gene="ttrB"
                     /locus_tag="PWX02_000283"
     CDS             888..1616
                     /gene="ttrB"
                     /locus_tag="PWX02_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase subunit TtrB"
                     /protein_id="HDL1728337.1"
                     /translation="MDLSKRSFLKELSALTVGASFVPLQSAQAFMPAWREGDENKRYA
                     MLIDLRKCIGCQACTVSCSVENATPLHSFRTTVRQYEITNGTQVVNNVVLPRLCNHCD
                     QPPCVPVCPVQATFQRKDGVVVINNEQCIGCGYCVQACPYDARFINEETKTADKCTFC
                     THRLEVGLLPACVESCVGGARVIGDLNDPKSQISQLYQAHKDDLKVLKPEAGTVPHVF
                     YVGLDDDAFVSKIDGQPMLWTGEA"
     gene            1618..2649
                     /gene="nrfD"
                     /locus_tag="PWX02_000284"
     CDS             1618..2649
                     /gene="nrfD"
                     /locus_tag="PWX02_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HDL1728338.1"
                     /translation="MIREVLVEPQHIVWLPWIVHYFFFVGVAATAVFTAVLFAKKSLK
                     PTACELAAVTVALIGSIVAPVALTADLHQPSRILHFYTDFAWWSPMAWGAIILPLFSV
                     AVAGYFVLALAHHTRPNLPKWLAWLQLPILQNQGLLWAFRLFATLTAIGIIGYTVLET
                     YQTGTRTLWHSAWLLPIMLFSAWAVALGLTQAVCKMLFHTQTNTGLWTKLCLILTALS
                     LVGLAFSSPQATQDFAQLFNGSITAYLVGIFWLIALICNFSAKNHRLQWLGVLALIAF
                     GWLLRWVLVIQVQTIAKTNALQNPYHFDWTAVDGGLGIVSIFGLTVLVSIVVWQLILI
                     ICGGRKGEQ"
     gene            2639..5734
                     /gene="ttrA"
                     /locus_tag="PWX02_000285"
     CDS             2639..5734
                     /gene="ttrA"
                     /locus_tag="PWX02_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase subunit TtrA"
                     /protein_id="HDL1728339.1"
                     /translation="MNNKRRNLLKGGLALGGAAAFGAGYSPKVSEIAKGVMHGTSGEK
                     TRDAIHGNSLTPEYQVKDGKLLSNPEQIVCNTQCMGCWTQCGIRARVDLNTNQVIRIS
                     GNPYHPLSSDKTLPFGMPIAKAELLLAGESGIENRSTACARGAAFLEGVNSPYRITQP
                     LKRVGKRGEGKWQTISFEQLVEEIVNGGNLFGEGEVEGLKTIRNLVDPINPKQPDLGM
                     KANQLLVTFAGPEGRQPILQRFAQKSFGTINFSNHGAYCGASYRAGSGAFMKDFDNQQ
                     HAKPDWDHAEFILFIGTSPAQSGNPFKRQARQLAKRRPDDNFSYVVVSPRLEMTSSLA
                     TQNNHWVPIKPEGDLALVMGMLRWIIENERFNADYLSRPSLAAMENAGHVSYANASHL
                     IICEPNHPKFGQALRISDLQDVQLDPEEKVEENLIVVKDQASGELISAKDCLQAVIFV
                     EQIAKLLDGSEVLVKSSMQLLKEATQEKTLAEYSDICGVPVQTIENLAKKFTSHGHKA
                     NAITHGGTMHSTGFYTSWAILLLNAMVGNLNKKGGMAVAAGKFKDFGDGPRYNLANFP
                     NEVKPKGTNLARSKKAYQNSSEFKQKVTKGENPYPAQGAWYPFTGGQTTEMFTSMVQG
                     YPYFAKAWINHMANPVYGLTAMNPIALEKLKDPKILPLFVSIDAFMNETTALADYIVP
                     DTHNFESWGFSTAWGGVPQRTSTARHPIVPSKNAKTAEGDTICMENFVIAVAKKMGLP
                     GFGENAIADLEGNRYPLHRTEDYFLRAAANIAFAGEKPAPDASEQDLLLTGVQRIMPK
                     IEQTLKAEERLKVANVYCKGGRFAPHESAWKEENMEFQWKNCLQIWNENVYKAQHHSN
                     GEHYWGCPRYMSPRFADGSTLEQHYPQSEWGFKLISFKSNIMSSITAPLLRLLSIKPE
                     GLVAMNRLDAEEKGIKHGDVVKLSTPTAGLTVQIVVLDGIARGTIGIEHGYGHKQIGA
                     SSYTIDGVEIPANPMIAKGINLNDLGIIDHTKAVKSPWVDWVCGSAVRNGIPAKVEKL
                     A"
     gene            5875..6720
                     /locus_tag="PWX02_000286"
     CDS             5875..6720
                     /locus_tag="PWX02_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL1728340.1"
                     /translation="MTFVKKTALSLIATALFATSSFAAEQSQYQHIRNATAKIEYAGK
                     TFLVDPYLAEKDRYLPPNAKKRNPTLDMTVSIDEVLNGVDAVIVTHTHHAHWDDVAQQ
                     RVPKNLPIFVQNAGDARLIRSQGFTDVRVVGKDTPFEGVTLTRTRGQHGTDAMYANPK
                     IAEMVGDAMDVVMKAEGKPTVYLVGDTIWNHEIEHSFNTYKPDFVVLNAGCAKLPNFE
                     GSPIMCTEDVSKAHKIAPQAKILTVHMDALPQTSISSDQMRDYVQKEGLNRVTVPKNG
                     DVIKF"
     gene            complement(6833..8230)
                     /locus_tag="PWX02_000287"
     CDS             complement(6833..8230)
                     /locus_tag="PWX02_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDL1728341.1"
                     /translation="MKLSKLAFYVALALTVTACTTGNMSNDGTLQQAQQTYQSYDEIT
                     KQYQINEQWWQGYTDAELNRVVDLAIKNNLDLAKSGIAVNKALYNANLVGANLVPTFS
                     GSGSVSAAKGLGSASSNNVSTGVSTTAAQAAFNLSYTVDLWRRLADTASAAEWTHKAT
                     IEDLKATRLSIINAVVTTYYQLAYYRDAIRITEESIRHYEQISRILTNKMNAGAIDSL
                     AVDQAQQAVLNARNSVIDLKTNQKIAEQTLRNLLNVQPNTPLNIRYPNIMSVKLQGVD
                     LNVPVSAIANRPDVVGSLQRLQSAFKTLTATEKSWFPTLTIGGSITGSATNIGNVADN
                     PVGNGLIKLDLPFLDWNRVKNNVKISETDYVTARLNYEQKITSALNEIDRYYYSYTQS
                     RSSLKILQDTNAKNKRISSYYKNRYEQGVSEFREWITALNTELNSQLAILNAKYAILT
                     NENAVYQAMAGKYRR"
     gene            complement(8320..8943)
                     /gene="gmk"
                     /locus_tag="PWX02_000288"
     CDS             complement(8320..8943)
                     /gene="gmk"
                     /locus_tag="PWX02_000288"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDL1728342.1"
                     /translation="MSLGNLYIISAPSGAGKSSLINALLADLPRSEVQLSISHTTRQP
                     RPAEQDGVHYYFTSHDEFKQLIEQGHFLEWAEVFGNYYGTSLPMIERSLEQGIDVFLD
                     IDWQGARQIREKVPNVKTVFILPPSKTELEKRLIGRGQDSAETIAKRMAEAVSEMNHY
                     NEFDYVIINDDFQQALAELKAILIAERLSLGSQQIRHQHLITDLLNN"
     gene            complement(9056..9529)
                     /gene="gpt"
                     /locus_tag="PWX02_000289"
     CDS             complement(9056..9529)
                     /gene="gpt"
                     /locus_tag="PWX02_000289"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL1728343.1"
                     /translation="MSEKYTNEKYVVTWDMFHMHARKLAENLLPATQWKGIIAVSRGG
                     LFPAAVLARELGIRHVETVCIASYDHDSQGELNVLHAADTDGEGFIVVDDLVDTGNTA
                     KEIRRMYPKARFVTVFAKPHGAPLVDDYVIDIPQETWIEQPWDLGITFVQPLARR"
     gene            complement(9674..11083)
                     /locus_tag="PWX02_000290"
     CDS             complement(9674..11083)
                     /locus_tag="PWX02_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL1728344.1"
                     /translation="MKKYPLLFSLFSPVVWAEKVIEHNTDPISFERQPQAVKFEQNVV
                     KNSQKPTAYQPAYSVNQNVEQQINQAILSQDWATLEPLLSRYPQTENFDPLLWHYAQA
                     ALAYAKKDHQTAVFHYRQLLAKPPSLVYPRFDLALILAEDQQFRAAEQEFKAIQAHLP
                     PELAKIARNTQVQIAENEAWQPDFYLQYTQTDNVNNASSSPIVNVNGRIFRKNADSLP
                     QSAKGVRYGLGLSKLHNVKGNHFVGLTLNYSGIYYWDNRDYREQSISVSPNYSYRTVA
                     YRLTFSPFIEQNWLGSSRYNVQFGATLNGLRYLNSQWLVSGSFTHLQKRYFEELTASR
                     YNSYQHQLSGGVQWQAVKNGRFFANLDGVREIAREKAQSSNKWLARVGAVFQGERWAT
                     QASIGFGKRYFADKHYLFGYKRLDTEYQANLAVWNRTWHWQGIVPKLNFRYQKIDSNI
                     ADFYPRQNKEVFFTLEKLF"
     gene            complement(11279..12139)
                     /locus_tag="PWX02_000291"
     CDS             complement(11279..12139)
                     /locus_tag="PWX02_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HDL1728345.1"
                     /translation="MSIKKVTLASLILLGLTACSSGGSGSSSNNVAKPNTKPQTTQQQ
                     TDKAAAEKAAKDKAAKEKAEAERLAAEKAAKEKAEAERLAAEKEVDPIAIIKAKGIDD
                     TNYGLKLGNSKETIEGKVTVRTQSYLYNQPYSVVTAQAVQRNGWENGNYIDKVDGTVT
                     VKGLKTEKLPTEGTATYSGKAFNHDGAKGFNGGDLTYNVNFSKRTGSGRVEGEFGNLI
                     VLEEGTINNTQISSTVHGNNPLNLSGKYTVEFFGKNAEEIAGEIQTKANLTGEVSNFG
                     LAGTRGDIQK"
     gene            12342..13796
                     /locus_tag="PWX02_000292"
     CDS             12342..13796
                     /locus_tag="PWX02_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HDL1728346.1"
                     /translation="MSEISTLAPQLLWQWFDKACAIPHPSYHENELAEFIVNWAKSKN
                     FFVERDEVGNVLIRKPATKGMENRQTVALQAHLDMVPQANTGTVHDFTKDPIQPYIDG
                     EWVKAKGTTLGADNGIGLASCLAVLDSDDLAHPTIEVLLTMTEEAGMEGAIGLRPNWL
                     TADIMINTDTEENGEIYIGCAGGENVNVTLPIFLVPHQQDSAVTVTLKGLNGGHSGCD
                     IHTTRANAIKVFARILEQAHNEVEFQIAEIQGGSVRNAIPREAHATLVFSAKNQQKLT
                     ACLSQTAEQVKAELKIAEPNFQFLIEEAQLPEQAFDLASTARVIHFLNVMPNGVVRNS
                     DVVENVVETSLSIGVLNTEASEVKATILARSLIEGGKEEIRSKIRSLTALVGAEVEFS
                     GNYPGWEPNPNSKITPLTKAIYDEILGYESQIKVIHAGLECGLINKIYPNMDFVSIGP
                     TILNAHSPDEKVHIPAVEIYWKLLTKLLEQAPEK"
     gene            complement(13871..15160)
                     /locus_tag="PWX02_000293"
     CDS             complement(13871..15160)
                     /locus_tag="PWX02_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1728347.1"
                     /translation="MDIRQKIAHNKMTSFQWLVVGLTVLLNMLDGFDLMALAFTAKSI
                     REELQLDGAAIGSLMSAGLFGMAIGSLFLSPLADKFGRRPLLLIGTGLATLGMLLTYF
                     ATSAFEIGVWRVLTGLGVAAILTGTNVLVSEYSSQKWRGLAIAIYASGFGIGAMLGGM
                     SAVLLQGEYGWRAVFLVGSILTGAVFVLLFFVLPESVEFLISKHPVNAKARLNQIAVK
                     LGLGNGWELPEIEAKSKSKVAITRLFEPEYRRNTLLIWLAFIAISASFYFISSWTPAL
                     LEEAGMPKTQSQTVGMAISVGGTIGSLIFGFLVSRWAAKHILQAFSILAASAVVAFVF
                     AGNLGLAITLAILVGGLINGCITGLYTINPTLYAPDFRSTGVGTAIGVGRLVSIVSPT
                     LAGILLDAGWKKNELYLGAAVVLLIAAFTVHFLKQRK"
     gene            complement(15364..15699)
                     /locus_tag="PWX02_000294"
     CDS             complement(15364..15699)
                     /locus_tag="PWX02_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HDL1728348.1"
                     /translation="MVSEQRQNPEEQKSEEQKINVEVLYAYPDKYFLKKLQVPQNATI
                     QTIILLSGVLEKYTEIDLRENKVGIFSRPAKLNETVEEGDRIEIYRPLIADPKEIRRK
                     RAAEQAEKK"
     gene            complement(15689..16123)
                     /locus_tag="PWX02_000295"
     CDS             complement(15689..16123)
                     /locus_tag="PWX02_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HDL1728349.1"
                     /translation="MPIVNQSALVPYSAEQMYQLVNQYEKYPEFLSGCVSTKTLSTGE
                     NELKAELHIQKLGISQTFSTHNKMTPPYKIEMALIEGPFRHLHGAWTFTPFDEQSCKI
                     ALQLNFEFSSPIVAMVFGKVFNELTLKMVNAFKQRAKEVYGV"
     gene            complement(16178..17233)
                     /gene="modC"
                     /locus_tag="PWX02_000296"
     CDS             complement(16178..17233)
                     /gene="modC"
                     /locus_tag="PWX02_000296"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HDL1728350.1"
                     /translation="MLKLKLLQQLGNLTLNVNLDLPNKGITAIFGRSGAGKSSLINLI
                     AGLSTAQAGFIELNGQVLFDSQNGINLPPEKRNIGYVFQEHRLFPHYSVEKNLKYGYK
                     RSDSADFLQIVELLGIAPLLNRYPASLSGGEKQRVAIGRALLSSPNILLMDEPLSALD
                     LPRKQELLDYLDKLSARLEIPILYVSHSLEEVVRLADHLVLLENGKVTAFDTLSAVWH
                     SSAFSAWQPNHQRISLLELPVEETLPHYNMLGLSLNAQRLWVKQFPRHQIGEKLRVTI
                     ASKEVSISLQKPTQSSIRNVLKGNVWKIETHLERVDLAIQVENQQIWSTISLWSFNEL
                     AIQPGQEVYLQIKSVSL"
     gene            complement(17220..17912)
                     /gene="modB"
                     /locus_tag="PWX02_000297"
     CDS             complement(17220..17912)
                     /gene="modB"
                     /locus_tag="PWX02_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDL1728351.1"
                     /translation="MLNFTPQELNAIVLSLKVASIAVVLALPLAVWVAWLLARKQFWG
                     KNLLNGIVHLPLVLPPVVIGYLLLISMAKRGVIGQYLWEWFHFSLSFSWKGAVLASMV
                     MAFPLMVRSIRLAFEAVDPKLEQAARTLGATPLKVFFTLTLPLSFSGIIAGGVLGFAR
                     SLGEFGATITFVSNIPNQTQTIPAALFTFIETPDGEMAAARLCLVAILISLIALFCSE
                     WLAERQKRYAQT"
     gene            complement(18158..18913)
                     /gene="modA"
                     /locus_tag="PWX02_000298"
     CDS             complement(18158..18913)
                     /gene="modA"
                     /locus_tag="PWX02_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL1728352.1"
                     /translation="MKALKKLAITMALTLSGTAFSQEITVFAAASMTNVLQDIQQDFE
                     KAYPQDKITFSFASSSVLAKQIEQDAPADIFISADQKWMDYVAEKQPEKVKYRRNLVR
                     NELVLIAPTESNIEPKAIQAVDFAKELANSYLSVGDPNHVPAGKYAKKALSHYQLWGS
                     VEPRLARAKNVRDALSFVERGESPLGIVYSTDAKVSNKVKVVSVFPPESYGEIVYPAA
                     AVSEKASAATFLDFLKTPKAKAKFEAAGFYPVN"
     gene            complement(18934..19695)
                     /locus_tag="PWX02_000299"
     CDS             complement(18934..19695)
                     /locus_tag="PWX02_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1728353.1"
                     /translation="MNLIGFYTLAYKECRRILRIWRQTLVPPVITTTLYFLIFGTLIG
                     GRIGEMNGVGYTQFIAPGLIMMTAITASYVNTASSFFLSKFTRDFEEMLISPLSTHNI
                     IWGYVTGSILRAGLAATLVMCVAMVFVSFEIYSVAIIVLTLLLTIIAFALGGVINAVF
                     ARSFDDVGLIPTFVLTPLTYLGGVFYSISLLPEFWQTVSKFNPIVYMINGLRYGFLGI
                     SDVPLGYTFAVLTSLCIGLYIIAYSLIQRGVGLRS"
     gene            complement(19697..20605)
                     /locus_tag="PWX02_000300"
     CDS             complement(19697..20605)
                     /locus_tag="PWX02_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1728354.1"
                     /translation="MKALELIDLVKTYPTGVKAVKGINLVVEQGDFYALLGHNGAGKS
                     TTIGIISSLVNKTSGSVKVFGYDLDTQKVELKHQIGLVPQEFNFNQFEKVIDVLVQQA
                     GFYGIPRSEALVRAEKWLKLLDLWEKRTHLTRELSGGMKRRVMIARALMHNPKLLILD
                     EPTAGVDIELRRSLWDFLRDLNNQGTTIILTTHYLEEAETLCRHIGIIQHGELIENTS
                     MKALLAKLESEVFVLDLQNSEQNQPLVIQNYPHKWLDENTLEVNVKRQQGLTNLFSQI
                     SAQGAEVLSMRNKSNRLEELFINMAQ"
     gene            complement(20611..22668)
                     /gene="dnaX"
                     /locus_tag="PWX02_000301"
     CDS             complement(20611..22668)
                     /gene="dnaX"
                     /locus_tag="PWX02_000301"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDL1728355.1"
                     /translation="MSYQVLARKWRPQRFSEVVGQQHVLSALENSLREDRLHHAYLFS
                     GTRGVGKTSIARLFAKGLNCVNGITAEPCGECANCKAIEEGHFIDLIEIDAASRTKVE
                     DTRELLDNVQYKPTVGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEYVKFLLATTDPQ
                     KLPITILSRCMQFHLRALEQSQIARHLEFILNAENIPFEPLALEKLAKAAQGSIRDSL
                     SLTDQAIAMSNANITLAIVSQMLGLIDDNQPLELVQATAQADGEKAMAIIEQIAAKGV
                     DWQQLLSDVAEVLHKIAMLQLVKTPTTEETLVHFLAKQIPPEDVQFFYQLILNSKKEL
                     PFAPEQRAGVEMAILRALAFHPKQAEKIATVAVEKVTQPNHSVSAVTTAKPQNQISQL
                     RENFEQNQQKTVTVANTTTAVESIAQPPQAVDTAISPSAPATLSPAMAALEARKKLQA
                     QQANLAQTEKKKPELKAPQVIAKKSANQTASLQERFISLAENQHKAVLQVSKKTESAP
                     KSVEDEDYRWTWLNPELENQADSAKPSDIKQAILQERTPELVAKTIHLSCQQDKWCDI
                     ANSLNLGGFSRQIALNSYLAEQNEQHLALVLKPNMAHLDNPESRKNLEAALAKKGYSY
                     ELTIGDNSEQQTPLEIRRAIFKQLTEAAKSALLNDEKLMLLRNAFDAQIDESTIRAVA
                     ETE"
     gene            complement(22759..23298)
                     /gene="apt"
                     /locus_tag="PWX02_000302"
     CDS             complement(22759..23298)
                     /gene="apt"
                     /locus_tag="PWX02_000302"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDL1728356.1"
                     /translation="MNQLDLIKSSIKSIPDYPKAGIIFRDITSLLEVPTAFQATIDAI
                     VAEFKDKGITKIVGTESRGFIFGAPVALALGVPFVLVRKPKKLPREVISQSYTLEYGE
                     DTLEIHKDAVSANDNVLVIDDLLATGGTIDATAKLIRRLGGNVEHAAFVIWLPELGGK
                     ERLEKEGIKSFSLVSFEGH"
     gene            complement(23426..24376)
                     /gene="lpxM"
                     /locus_tag="PWX02_000303"
                     /gene_synonym="msbB"
     CDS             complement(23426..24376)
                     /gene="lpxM"
                     /locus_tag="PWX02_000303"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249087.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HDL1728357.1"
                     /translation="MTRITAQDGYTHKFLWSFLHPKYWGVWLGVLGLVILAYVPVRIR
                     DKLSAFVGKMAYRYLKQKNKKGYHRAKVNLRYCFPDWSEKKREEVVEKMFITVAQTML
                     AIGETAIRPASYLQKRCEFTGFENVVKAKESGKNVIMLVPHTWSIDMAGVAMFSLGYP
                     MTSMYNPHRNALVDWLWNITRERLGGRMHTRQNGIKAFLADVRKGDVGYFLPDEDFGE
                     ELSVYADFFATEKATLPGLNKMARVANAEVIPMFSIYNAEKAKYEMEILPPLEFSGDP
                     IQSAREMNRVIEYFVEKNPEQYVWILRLLKTRRNREDIYL"
     gene            complement(24817..25500)
                     /locus_tag="PWX02_000304"
     CDS             complement(24817..25500)
                     /locus_tag="PWX02_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HDL1728358.1"
                     /translation="MEREQQIPVTNIDVAPKQSSVWKTIFADGVWKNNGALVQLLGLC
                     PLLAVSNSVTNALGLGLATMFVLICTNITVSLFRKITPHDIRIPIYVMVIATVVTAVQ
                     LLMNAFAFPVYQSLGIFIPLIVTNCIVIGRAEAYASKNEVKFSAFDGFSMGLGMTFSL
                     VVLGALREIIGNGTLFSGLDLLLGDWAKALRIDVLHIDSGLLLAILPPGAFIGLGVIL
                     AVKNIIDKR"
     gene            complement(25509..26141)
                     /gene="rsxG"
                     /locus_tag="PWX02_000305"
     CDS             complement(25509..26141)
                     /gene="rsxG"
                     /locus_tag="PWX02_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HDL1728359.1"
                     /translation="MNTSKITLNYALILGFIALLCTAVSTGVYLLTKGKIDDVTAMQQ
                     RKLLEEVVPKSHFDNDVLATCKLVQLENAPYLNRIYVAKKSENLTAYLVQGTAPDGYS
                     GDIVLLMGIEPNGNILGVRTLEHKETPGLGDKIETRVSDWILSFTNQLFSLENEADWN
                     VKKDGGKFDQFTGATITPRAVVNHIRQSAKWVVTELAQNPTMFNQFKACK"
     gene            complement(26141..27196)
                     /gene="rsxD"
                     /locus_tag="PWX02_000306"
     CDS             complement(26141..27196)
                     /gene="rsxD"
                     /locus_tag="PWX02_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HDL1728360.1"
                     /translation="MFKMVSSPHTHSSNLTANFMLWVIAMILPAFGVQLYYFGYGVLI
                     QTLLAVSLAIVIEIGIAKLRKKTTAFYLADLSGILTALILAMSIPPYAPYWLVLIGVI
                     VALLLAKHSYGGLGQNLFNPAMVGYALLLVSFPVQMTAWIVPIELLSEPPTFRDAISL
                     IFYGVTSDGFSIHQLVNSVDGIAQATPLDSMKSSLAKSGFDAVMNTPIFNGLFATGWL
                     QLNLAFLFGGLVLIYKRIIHWQIPVAMLATFAFFSGFTELFSQQSHLHFVSHFFSGAM
                     MFGAFFIATDPVTASITPRGKLIFGGLVGLLVYLIRYYGNYPDAIAFSVLLANICVPL
                     IDHYTQPRLYGTKLDNR"
     gene            complement(27242..27877)
                     /locus_tag="PWX02_000307"
     CDS             complement(27242..27877)
                     /locus_tag="PWX02_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HDL1728361.1"
                     /translation="MFSELIPALPWSFLFIQDLIGTIVFAISGAIAARQHKMDIFGMF
                     ILAFVTGVGGGTLRDMMIGSTPVFWMKQPIYVLMITLAVVITAIFRNQISRKSWQTGL
                     LVFDAIGLGVFTVIGVQKGLDFGLHPLICVALGGVTGCFGGLIRDILRNEVPIILQKE
                     VYVTASLIGGVIFVAANSVGLESDWVYLATVFTVIIIRLLAIRFNLNLPRL"
     gene            complement(27870..30164)
                     /gene="rsxC"
                     /locus_tag="PWX02_000308"
     CDS             complement(27870..30164)
                     /gene="rsxC"
                     /locus_tag="PWX02_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDL1728362.1"
                     /translation="MSNPNLVIERIRQNKETGKLWDFPGGIHPMENKSQSNSKPIRTL
                     NLPKFFYVPLVQHSGWAGELLVKVGDVVLKGQPLTKGDHFRQLPVHSPTSGRVIGIEP
                     YVSAHASGLPEITVIIETDGKDKWVERQPIEDFLSLTSDQIIQKVYQYGIAGLGGAVF
                     PTATKLSLADKRCKLLVINGAECEPYITCDDRLMQDFTADLIEGIRILRYVLRPEEVV
                     IAIEDNKPHAIQALETALKGSNDMFVRAIPTKYPSGASDQLIQVLTGLEIPAGKRTIE
                     MGIVMHNVGTAFAVKRAIIDDEPLIERVVTLTGDKIRNKGNVWARLGTPIQHLLEQVD
                     YQADKRFPVFLGGPMMGFILPALQAPITKTANCLIAPDHFEYAPPEPERSCIRCSSCS
                     DACPVGLLPQQLYWFARAEDHDKSKEYHLDACIECGVCAYVCPSYIPLIQYFRQEKAK
                     IEEIEEKARKAEEAKIRFEAREARLQKERDARTARISQAAEKRREEVANAGGVDPVKA
                     ALERLKAKKAVETVQEIPAEVQVKPNGEPDNSDLMAQRRARRLAKQAEQAVEKSENVT
                     NALIGANNDLPLQSMNETASDKSVGANNYSPEAESEDPRKAAVAAALARAKAKKQAQQ
                     AVEKSENITNAVIGVNNDLPLQSMNETVSDKSVGANNHSPETESEDPRKAAVAAAIAR
                     AKAKKQAQQAVEKPEDIANAVIGVNNDLPLQSMNETTSDKSVGANNHSPKTESEDPRK
                     AAVAAAIARAKAKKAAQQEKSNNV"
     gene            complement(30151..30768)
                     /gene="rsxB"
                     /locus_tag="PWX02_000309"
     CDS             complement(30151..30768)
                     /gene="rsxB"
                     /locus_tag="PWX02_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HDL1728363.1"
                     /translation="MLDLPIIAYILIAIAVIALIFGAVLGYSSIKLKVESDPIVDQID
                     ALLPQSQCGQCGYPGCKPYAEAIANGDVITKCVPGGQPLVVNLAELLGVEVPPMEGMV
                     EPEMKVAFVIEDLCIGCTKCIQACPVDAIIGTNKAMHTIIPDLCTGCELCVAPCPTNC
                     IEMRPIKPTTQSWNWKFDQDLIIPIVNTTELQRKLVTGGGKNEQS"
     gene            complement(30768..31349)
                     /gene="rsxA"
                     /locus_tag="PWX02_000310"
     CDS             complement(30768..31349)
                     /gene="rsxA"
                     /locus_tag="PWX02_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDL1728364.1"
                     /translation="MVDYILLVISTALINNFVLVKFLGLCPFMGVSKKVETAIGMGLA
                     TTFVLTVASLSAYLMETYLLEPLNAQFLRTLVFILVIAVIVQLTEMIVHKTSPTLYRL
                     LGIYLPLITTNCAVLGVALLNVNLANNLVESVLYGFGAAAGFSLVLVLFAALRERLAA
                     ADVPRPFQGASIALITAGLMSLAFMGFTGLVKL"
     gene            complement(31664..32149)
                     /locus_tag="PWX02_000311"
     CDS             complement(31664..32149)
                     /locus_tag="PWX02_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728365.1"
                     /translation="MAGLLPPYFKKKNSDHYKDAVKQFGKEQVDEVIAQCDELESWVK
                     EMPTKQLIMYVGCFHTFNKLSQSKAYVQENFPNVLENPFLFQTLSAASHNELKINRGY
                     KFKEKMLDEFYNLIELSEKDDEEYSEEEVKLPLWKKIYYVFIIFGIIMFIYRVINWLL
                     F"
     gene            complement(32177..>32472)
                     /locus_tag="PWX02_000312"
     CDS             complement(32177..>32472)
                     /locus_tag="PWX02_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728366.1"
                     /translation="DTRYNKNGQGAVSERNIIDAKNRFKASHPYAKIISCVRGSETYV
                     ARPAKANATTKSVKEKQSQPQESSSLSSMLLGAAVTAGVGFVANKWFSKKDNT"
BASE COUNT         9171 a         7351 c         6945 g         9005 t
ORIGIN      
        1 aaatcaagat tatattaaaa acagaaaggg tatggatgat ttaaaatcca ttaatacaga
       61 taatgataac atgcctgttt cattgacttt ttatgatgta ttaaaatata taaattggtt
      121 taatcaaaaa cataatgcaa atgtaaggct attaaatttt gatgaatata agtttatttc
      181 tccgtttgaa caaacagata gaaatgaatg gaagcatgaa gatatagaat ttatatatga
      241 tgagaaaata tctacagaac cacctcctta tatggaggaa gaaaaatttc aaaaaataat
      301 aatgaggttt tcaaaagata taaaaataat gatgcacaac cacataaact tcatagaatc
      361 tgacagattt gcggaatgga ttcttgaaaa ggcttgtgtt cgctctaaaa cattaaccag
      421 cttttatggc gacaagtctg ttataagggc cgttccacca ttggattgtt caggaaaata
      481 taaatatacc aaaataggat ttagattatg ctacgatttg tagggggata tatgaagaaa
      541 tacagtaatg attctcttat aaatagtatt gttatatcta aattaaaagc aggatggaaa
      601 attagaaaag gaaagaaaca taatatatta attgccccaa attctaggag attagcgata
      661 cctagtaccc cgagtgatat taatgcgtat aaaaacttta gatctaaact aaaccatctt
      721 attgcttttt aattagtatc aatgccctat tatctacttg tatagggcat ttcccttcat
      781 ccttgaccca aatcaacttt ccccttgata tgtggaaaac cacaatacct tgccgttccc
      841 tctccgcctt agaatacctt tgttattttt aaatttgtgg gggaaatatg gatttaagca
      901 aacgttcctt tttgaaagag ctttccgcct tgacggtggg ggcgagtttt gtgccgttgc
      961 aatcggccca ggcgtttatg ccggcttggc gtgagggcga tgagaacaaa cgctatgcga
     1021 tgctgattga tttgcgtaaa tgtatcggct gtcaagcgtg taccgtgagc tgttcggtgg
     1081 agaacgccac gccgttgcac agtttccgca ccacggtgcg tcaatatgaa atcaccaacg
     1141 gcacgcaggt ggtgaacaat gtggtgttgc ctcgtttgtg taaccattgc gatcaaccgc
     1201 cgtgtgtgcc ggtctgcccg gtgcaggcaa cctttcaacg taaagacggt gtggtggtga
     1261 ttaacaatga gcagtgcatc ggctgtggtt actgcgtgca agcctgcccg tatgatgccc
     1321 gttttatcaa tgaagagacc aaaaccgccg ataaatgcac cttctgcact caccgtttgg
     1381 aggtaggtct gctgccggcg tgcgtggaaa gttgcgtggg cggggcaagg gtgattggcg
     1441 atttgaacga cccgaaaagt caaatttccc aactttatca agcccacaaa gatgatttga
     1501 aagtcctgaa accggaggcc ggcaccgtgc cacacgtttt ctatgtgggg ctagacgatg
     1561 atgcctttgt gagcaaaatt gacgggcaac caatgctctg gacgggggag gcgtaaaatg
     1621 attcgtgaag tattagtgga accgcaacat atcgtctggt tgccgtggat tgtgcattat
     1681 ttcttcttcg taggcgtggc ggcaacggca gtgtttaccg ccgtgctgtt tgcaaaaaaa
     1741 tcactgaaac cgaccgcttg cgagctggcg gcggtgacgg tagccttgat tggctcgatt
     1801 gtcgcccctg tggcattgac tgccgacctg caccaaccga gccgtattct gcatttctac
     1861 accgattttg cgtggtggtc tccaatggcg tggggagcga ttatcctgcc cctgttcagc
     1921 gtggcggtag caggttattt tgtgctggca ttggctcatc atacccggcc gaacctgccg
     1981 aaatggttag cgtggctaca gttgccgata ctgcaaaatc aaggcttgct ttgggcattc
     2041 cgcctgtttg ccacactcac cgctatcggc attatcggct acaccgtgct tgaaacctac
     2101 caaaccggca caagaacctt atggcatagt gcgtggctgc tgccgattat gctcttttct
     2161 gcgtgggcgg tggcattggg gcttacccaa gcggtctgca aaatgctgtt ccacacccaa
     2221 accaacacgg ggctatggac aaaactctgc ctgattttaa ccgcactttc gcttgtcgga
     2281 ctagcattca gtagtccaca agccacacaa gattttgccc agctcttcaa tggctcaatc
     2341 accgcttacc ttgtcggcat tttctggcta attgccctca tttgcaattt ttctgccaaa
     2401 aaccaccgct tgcaatggct cggcgtgctt gccctcatcg ctttcggctg gctactccgc
     2461 tgggtactgg tgatccaagt ccaaaccatt gccaaaacca atgccttgca aaatccatat
     2521 catttcgatt ggacagccgt tgatggtggt ttggggattg tctcgatttt tgggctaacg
     2581 gtgctagtaa gtatcgtggt atggcaactg attttaataa tttgtggggg aagaaaaggt
     2641 gaacaataaa cgcagaaatc tgttgaaagg cgggctggca cttggtgggg cggcggcgtt
     2701 tggggccggg tattcgccga aggtgtcgga gattgccaaa ggggtgatgc acggcacatc
     2761 gggcgagaaa acccgtgatg caattcacgg taattcgctc acgccggagt atcaggtaaa
     2821 agacggcaaa ctgctcagca atcctgagca aattgtgtgc aatacacagt gtatgggctg
     2881 ttggacgcag tgtggcattc gggcgagggt agatttgaac accaaccaag tgattcgcat
     2941 ttcgggcaat ccgtatcacc cgctttcctc cgacaaaacc ctgccgttcg gtatgccgat
     3001 tgccaaggcg gaactgttgc tggcgggcga aagtggcatt gaaaatcgct ccaccgcctg
     3061 tgctagaggg gcggcgttct tggagggggt gaacagcccg tatcggatta cccaaccgtt
     3121 gaaacgggtc ggaaaacggg gcgaaggcaa gtggcagacc atcagctttg agcagttggt
     3181 ggaggaaatc gtcaatggtg gcaacctatt tggtgaaggc gaagtggagg gcttgaagac
     3241 gatccgcaat ctggtcgatc cgattaaccc gaaacagcct gatttgggaa tgaaagcaaa
     3301 ccagctttta gttacttttg ccggccccga aggtcgtcag ccgattttgc aacgctttgc
     3361 ccaaaagagc ttcggcacga tcaacttttc caaccacggg gcttattgtg gcgcttcgta
     3421 ccgtgccggt tcgggtgcct ttatgaaaga ttttgacaac caacaacacg ccaagccgga
     3481 ctgggatcac gctgaattta ttctgtttat cggcacgtct ccggcacagt cgggcaatcc
     3541 gtttaagcgt caggctcgcc agcttgccaa acgccgccct gatgacaatt tcagctatgt
     3601 ggtggtttcc cctcgtttgg agatgacctc ctccctcgcc acccaaaaca accattgggt
     3661 gccgattaag cccgagggcg atttggcgtt ggtaatggga atgttgcgtt ggattatcga
     3721 aaacgagcgt ttcaatgctg actacctctc ccgcccaagt ctggcagcga tggaaaatgc
     3781 cggccacgtc agctatgcca atgcgtcaca tctgattatt tgcgaaccga accacccgaa
     3841 attcgggcag gcgttgcgga ttagcgatct gcaagatgtg caactcgacc ccgaagagaa
     3901 agtggaagaa aacctgattg tggtaaaaga tcaggcaagc ggcgaactca tttcagcaaa
     3961 agattgctta caagcggtca tttttgtcga acaaattgca aaactgcttg atggctcgga
     4021 agtgttggtg aaatcctcaa tgcagttgct gaaagaggcg acacaagaga aaacccttgc
     4081 cgaatatagc gacatttgtg gcgtgccggt gcaaaccatt gaaaacttag cgaaaaaatt
     4141 cacttcgcac ggtcataaag ccaatgccat cactcacggc ggcacgatgc acagcaccgg
     4201 tttttatacc agttgggcga ttttgttgct gaatgcaatg gtcggtaacc tcaacaaaaa
     4261 aggcggaatg gcagtagccg caggcaaatt caaagatttc ggggacgggc ctcgctacaa
     4321 tttagcgaac ttcccgaatg aagtgaagcc aaaaggcacg aaccttgccc gcagtaaaaa
     4381 ggcgtaccaa aattcaagcg aatttaagca gaaagtgacg aagggcgaaa atccgtatcc
     4441 ggcacaaggg gcgtggtatc cgttcaccgg cgggcaaact accgagatgt ttacctcaat
     4501 ggtgcagggc tatccatact ttgcgaaagc gtggatcaac catatggcga accccgttta
     4561 cggcttaacc gctatgaacc cgattgcgtt ggagaaatta aaagatccga aaattctacc
     4621 gctctttgtc tctattgatg cttttatgaa cgaaaccact gcactggcgg actacatcgt
     4681 gccggacacc cacaactttg agagctgggg cttttcaacg gcgtggggcg gtgtgcctca
     4741 acgcaccagc accgcacgcc acccgattgt gccgtccaaa aacgccaaaa ctgccgaagg
     4801 cgacaccatc tgtatggaga atttcgtgat tgcggtggcg aaaaaaatgg gcttgccggg
     4861 ctttggcgaa aatgcgattg ccgatttaga aggcaaccgc tatccgctcc accgtaccga
     4921 agattacttc ctacgggcgg cagcgaacat tgcctttgcc ggtgagaaac ctgcaccgga
     4981 cgccagcgag caagatctgc tgctcaccgg cgtgcaacgg attatgccga aaattgaaca
     5041 aacgctaaaa gccgaagagc gattaaaagt ggcgaatgtg tattgcaaag gcggacgttt
     5101 tgccccacac gaaagtgcgt ggaaagagga gaatatggag ttccagtgga aaaactgcct
     5161 gcaaatttgg aacgaaaacg tgtataaagc ccagcaccac agcaacggtg aacactattg
     5221 gggctgccct cgttatatgt cgccacgttt tgcggacggc tccacgctgg agcagcacta
     5281 tccgcagtcg gaatggggct ttaaactgat ttcgtttaaa tcgaacatta tgagcagtat
     5341 taccgcccca ctgttgcgtc tgctctcgat caagcctgag ggcttagtgg cgatgaaccg
     5401 tttagatgcg gaagaaaaag gcattaaaca cggcgatgtg gtgaaactca gcaccccaac
     5461 cgccggcttg acggtgcaga ttgtggtgtt ggacggtatc gcccgaggca caatcggcat
     5521 cgaacacggc tacggtcata aacagattgg ggcaagttcc tacaccattg acggcgtgga
     5581 aattccagcc aacccgatga tcgccaaagg gattaacctc aacgacctcg gcattatcga
     5641 ccacaccaaa gcggtcaaat ccccgtgggt agattgggtc tgtggctcag cggtgagaaa
     5701 tggcattccg gcgaaggtag agaaattagc ctagccgaac aagcggttgg ttttgcagaa
     5761 aaatgtgcaa aatcgaccgc ttttattgat gtaagggaca ctgttcctta tgcaaaccag
     5821 cattgcagag cgtaaaaaat catactctgc attttaattt attttggaga acctatgact
     5881 tttgtcaaaa aaacagcact gagcctgatt gcaacggcac ttttcgctac atcaagtttt
     5941 gccgctgagc agagccaata ccaacatatt cgtaatgcaa cggcgaaaat tgaatatgcc
     6001 ggcaagacgt ttttggttga tccgtattta gcggagaaag atcgctactt accgccgaat
     6061 gcgaaaaaac gtaatccgac cttggatatg actgtgagca ttgatgaagt gttaaatggc
     6121 gtggatgcgg tgatcgtaac tcacactcac cacgctcact gggacgatgt agcacaacaa
     6181 cgtgtgccga aaaatctgcc gatttttgtg cagaatgccg gtgatgcccg tttaatccgt
     6241 tcacaaggct ttaccgatgt gcgtgtggtc ggcaaagata ccccgtttga aggcgtaacc
     6301 ttaacccgaa ctcgtggcca gcacggcacc gatgcgatgt atgcgaaccc gaaaattgcc
     6361 gaaatggtcg gcgatgcgat ggacgtagtg atgaaagcgg aaggcaaacc gaccgtctat
     6421 ttggtgggag ataccatttg gaaccacgaa atcgaacatt cgttcaacac ttacaaacca
     6481 gactttgtgg tgctgaatgc cggctgtgcc aaactaccaa actttgaagg cagcccgatt
     6541 atgtgtaccg aagatgtttc caaagcacac aaaatcgctc cacaagcgaa aatcttaact
     6601 gtgcatatgg acgccctgcc acaaacctca atcagcagtg atcagatgcg tgattatgta
     6661 caaaaagagg gattaaatcg tgtaactgtg ccgaaaaacg gtgatgtgat taagttctag
     6721 tagctaaact aacattacca aaatggttta agtagcaata aatagcgaag cgggttaagc
     6781 catcaagcct taacccactt ttaccaacca tagtatatag ttttagatat aattatcttc
     6841 tgtatttacc tgccattgct tggtaaaccg cattctcgtt ggtcaaaatc gcatattttg
     6901 catttaaaat cgccaactga gaattaagct cggtattcag tgcggtaatc cactcacgga
     6961 actccgacac cccttgttca taacggtttt tatagtatga actgatacgt ttatttttcg
     7021 cattggtatc ttgaaggatt tttaacgagc ttctcgactg cgtataactg taataataac
     7081 ggtcaatttc atttaaggct gaggtaattt tttgctcata gtttaaacgg gcagtcacat
     7141 aatccgtttc agaaattttt acgttatttt taacccgatt ccaatctaag aacggtaaat
     7201 ccaatttaat taagccgtta cccacagggt tatctgccac attgccgata ttggttgcac
     7261 taccggtaat agaaccaccg atggttaagg ttgggaacca gcttttctcg gttgcggtta
     7321 aggttttaaa tgcactttgt aagcgttgta acgagcctac tacatcaggg cgattagcaa
     7381 tggctgacac tggcacattt aaatctaccc cttgcaattt cacgctcata atattcgggt
     7441 aacgaatgtt aagcggagta tttggctgaa catttaacaa attgcgtaat gtttgctcgg
     7501 caattttttg gttggttttc agatcaatta cactatttct ggcgtttaag accgcttgtt
     7561 gagcctgatc aaccgctaaa ctatcaatcg caccggcatt cattttgtta gttaaaatac
     7621 ggctgatttg ctcataatgg cgaatgctct cttcggtaat acgaatcgca tcacgatagt
     7681 aagctaactg gtagtaagta gtcacaaccg cattaataat agataaacgg gtagctttta
     7741 agtcttcaat ggtggcttta tgcgtccatt ctgccgcact ggcggtatcg gctaaacgtc
     7801 gccataaatc cacagtataa cttaaattaa atgcagcttg agctgcggtt gttgaaacac
     7861 cggtcgaaac attatttgaa gaagccgaac ctaaaccttt agccgcagaa acagaacctg
     7921 aaccactaaa ggttggcact aaatttgccc ctactaaatt agcgttatac aaggctttat
     7981 tgactgcaat tccgcttttg gctaaatcaa ggttattttt gatggctaaa tcaaccacac
     8041 ggtttaattc cgcatccgta tagccttgcc accattgctc gttaatttga tattgtttgg
     8101 taatttcatc atagctttga taggtttgct gtgcttgttg caaagtcccg tcattgctca
     8161 tattccctgt ggtacaagcc gttacggtta gagccaacgc cacataaaat gctaattttg
     8221 aaagtttcat tacatttcct tttgaaaata aagaggggaa aatgggcgta ttctacccaa
     8281 aaacagattg cctttccagt taaatgaaca actgttcatt taattattta acaaatcagt
     8341 aatcaaatgc tgatgacgaa tttgttgcga acctaagctc aaacgctcgg caattaaaat
     8401 cgcttttagc tctgccaatg cctgttggaa atcatcatta ataatcacat aatcgaactc
     8461 attgtaatgg ttcatttccg acaccgcctc tgccatacgt ttggcaatgg tttcagcgga
     8521 atcttgccca cgcccgatta aacgtttttc caattctgtt tttgacggtg gcaaaataaa
     8581 aacggttttc acattcggca ctttttcccg aatttgtcta gccccttgcc agtcaatatc
     8641 caaaaagaca tcaatgccct gctctaggct acgttcaatc atcggcaatg atgtgccgta
     8701 ataattgcca aacacctccg cccattctaa aaaatgccct tgctcaatca gctgcttaaa
     8761 ctcatcgtgc gaagtgaaat aataatgcac gccgtcctgt tctgccggac gaggctgacg
     8821 ggtagtgtgt gaaatcgaaa gctgtacttc cgaacggggt aaatccgcca acaaggcgtt
     8881 aattaaggaa gattttcctg ctccacttgg ggcagaaata atataaagat tgcctaaact
     8941 cattatttgt atatacctat ttgattttgg gaatagggcg aatgatgatt cgcccctaca
     9001 tttcatagga ataaataaaa aataatggac aactttcatt gtccattaca caaaattatc
     9061 tacgtgccaa aggctgaacg aaggtaatgc ctaaatccca aggctgctca atccacgttt
     9121 cttgtggaat atcaatcaca taatcatcaa ccaacggtgc accgtgcggt ttcgcaaaca
     9181 cggttacaaa acgagctttc gggtacattc tgcgaatttc ttttgccgtg ttgccagtat
     9241 ccactaaatc atctaccaca ataaagcctt cgccatcggt atcggctgcg tgtaacacat
     9301 ttaactcacc ttggctatcg tgatcgtagc tggcaatgca cactgtttct acgtggcgaa
     9361 tacccaactc acgtgctaaa acggctgccg gaaacaagcc gccacggctc accgcaataa
     9421 tccctttcca ctgtgtggca ggtaataaat tttctgccaa cttgcgagcg tgcatatgga
     9481 acatatccca cgtcacgaca tatttttcat ttgtgtattt ttcactcata gaaacaacct
     9541 aatataaaat tgggggctac cccgatataa aaaatcgcac taggataacc cgaaaatgct
     9601 tttttcgcca tcatttcatt tattttttgt gccggcagct acgcaatcgt atgcttagca
     9661 caaaaaacca acactaaaat agcttttcta aagtgaaaaa gacttccttg ttttggcgag
     9721 ggtaaaaatc agcaatattg ctgtcaattt tttgataacg gaaattcagc ttcggcacaa
     9781 tgccctgcca atgccaagtg cggttccaaa ccgctaaatt ggcttgatat tccgtgtcta
     9841 accgcttata gccaaacaaa tagtgcttgt cggcaaaata tcgcttgcca aagccgatac
     9901 ttgcctgcgt tgcccagcgt tcgccttgaa aaaccgcccc cactcgagcc agccacttat
     9961 tcgagctttg tgctttttcc cgtgcaattt cacgcacgcc gtctaaattt gcaaaaaacc
    10021 gcccattttt gaccgcttgc cactgcacac cgccactgag ttggtgctga tagctgttat
    10081 aacggcttgc ggttagctcc tcaaaatacc gtttttgcaa atgggtaaaa ctccccgaca
    10141 ccaaccattg cgaattaagg taacgcaaac cgttgagtgt tgccccaaat tgaacattat
    10201 aacggctact ccccagccaa ttttgctcaa taaaaggcga aaaagtcagc cgataagcga
    10261 cggtgcggta gctataattc ggcgaaacgg atatggattg ctcacggtaa tctcggttat
    10321 cccaatagta aatcccgcta taattgagcg ttaaaccgac aaaatgattg cctttcacgt
    10381 tgtgcaattt actcaaccct aagccataac gcacgccttt tgctgattgt ggcaaactgt
    10441 ctgcattttt gcggaaaatc cgcccattta cattcacaat cggcgaagat gaggcattat
    10501 tcacgttatc tgtttgggta tattgcagat aaaaatcggg ctgccacgcc tcattttcag
    10561 caatttggac ttgagtattg cgggcaattt tagcaagttc cggcggtaaa tgggcttgaa
    10621 tagccttaaa ttcctgctct gccgcacgaa attgctgatc ttctgccaaa atcaacgcca
    10681 aatcaaaacg agggtaaacc aaactcgggg gcttagctaa cagttggcga tagtgaaaaa
    10741 ctgcggtttg atgatctttt ttcgcatagg caagtgcggc ttgcgcatag tgccacaaca
    10801 acgggtcaaa attttcagtt tgcggataac ggcttaaaag cggttcaagc gttgcccaat
    10861 cttggctgag aatcgcttgg ttaatttgct gttcaacatt ctgattgact gaatacgcag
    10921 gctgataagc ggtcggtttt tgtgaatttt ttacaacatt ttgctcaaat ttaaccgctt
    10981 gcggctgacg ctcaaaggaa atcgggtcag tgttgtgttc aatcaccttt tctgcccata
    11041 caacggggct gaacagcgaa aataaaaggg gatatttttt cataaagaca aagaaaattg
    11101 gtaggggcgg acctatatgt tcgcctctac gggatttatc actctctccc cttgtgggag
    11161 agggttatta gaagctgtaa attgaaatct aaactattgt gtcttatggt atctacaagc
    11221 aatatcaaga caaatgccgt ttaatttttc gttaaacggc attgaacgtt agaaaaggtt
    11281 atttttggat atcgccacga gtgccggcaa gtccaaaatt actcacttcc ccagtaagat
    11341 ttgcttttgt ctgaatttca ccggcaattt cctctgcatt tttaccgaaa aattcgactg
    11401 tatattttcc agaaaggttt aaaggattat tgccatgtac tgttgaagaa atctgagtat
    11461 tattaatcgt cccctcttct aatacgatta agtttccaaa ttctccctca actctacctg
    11521 aaccggtacg cttagagaaa ttaacattat aggttagatc accaccatta aaacctttag
    11581 ctccatcgtg gttaaatgct tttcctgaat atgttgcagt accctctgtt ggtaatttct
    11641 ctgttttcaa tcctttaact gtaactgttc catcaacttt atcaatataa ttaccgtttt
    11701 cccagccatt tctttgaact gcttgtgctg ttactacaga atatggctga ttatataaat
    11761 aactctgtgt tctgactgtg actttaccct caatagtttc tttactatta ccaagtttta
    11821 agccgtaatt tgtatcatca ataccttttg ctttaataat agctatagga tctacttctt
    11881 tttctgctgc taaacgctcc gcttccgctt tctctttcgc cgctttttcc gccgctaaac
    11941 gttctgcttc tgctttctct ttcgctgcct tgtcttttgc tgctttttct gctgccgctt
    12001 tatcggtttg ttgctgagta gtttgcggtt tagtattcgg ttttgccaca ttgtttgagc
    12061 tactgcccga accaccacta ctacacgccg ttaaacccaa taaaatcaaa ctggctaaag
    12121 ttactttttt gatagacata aaatgttcct tttgtaagat aaacaaaaat agatgtgtat
    12181 aaaacacacg catattgtac aaaaaaaaaa caactcgtca tcaagtactt tttaagattt
    12241 ttttgttaaa tttctcctaa atattctaca atgtcagagg tctatatcag cttgaataca
    12301 agcggttatt ttttcttaaa attttacaaa aggggcttat tatgtctgaa atctcaacgc
    12361 tcgcaccgca gctactttgg cagtggtttg ataaagcctg tgctattccg cacccgtctt
    12421 accacgaaaa tgaactggct gaatttattg tgaattgggc aaaatcgaaa aatttctttg
    12481 ttgaacgtga tgaagtgggt aatgtgttaa tccgtaaacc ggcaactaaa ggtatggaaa
    12541 accgccaaac tgtcgctctg caagcccatc ttgatatggt gccacaagcc aataccggca
    12601 cggttcacga tttcactaaa gatccgattc agccttatat tgacggcgaa tgggtaaaag
    12661 ccaaaggcac aacattgggg gcagataacg gcattggatt ggcttcgtgc ttggcggtgt
    12721 tggatagcga tgatttagcc cacccaacca ttgaagtgtt gctcacaatg accgaagaag
    12781 ccggtatgga aggggcgatt ggtttacgcc cgaactggct aaccgccgat attatgatta
    12841 acaccgacac cgaagaaaac ggggaaattt acatcggctg tgcaggtggg gaaaatgtga
    12901 atgtgacact gccaattttc ttggtgcctc atcagcaaga tagtgcagta accgttacgc
    12961 ttaaaggcct aaacggcggg cattcgggct gtgatattca caccacccga gcgaatgcga
    13021 ttaaagtgtt cgcccgcatt ttggaacaag cccacaacga agtggaattc caaattgcag
    13081 aaattcaagg cggttcggtg cggaatgcca ttccacgaga agcccacgca accttggttt
    13141 tctctgcaaa aaatcagcaa aaattaaccg cttgtttaag ccaaaccgcc gaacaagtaa
    13201 aagcagaact caaaattgcc gagccaaact tccaattttt aattgaagaa gctcaactgc
    13261 ctgagcaagc ctttgacctt gccagcactg ctcgagtaat ccatttctta aatgtgatgc
    13321 caaatggtgt ggtgcgtaat agcgatgtgg tggaaaatgt ggtggaaacc tcactcagta
    13381 ttggtgtgct taacaccgaa gcgagtgagg tgaaagcgac cattcttgcc cgctcattaa
    13441 ttgagggcgg aaaagaggaa attcgcagta aaatccgctc attaaccgca ttagtgggag
    13501 cagaagtgga attttcgggc aactacccgg gctgggagcc aaacccaaat tccaaaatca
    13561 cgccattaac caaagcgatt tatgatgaaa ttttaggtta tgaatctcaa attaaagtga
    13621 ttcacgcagg gctagaatgt ggcttaatca acaaaatcta cccgaatatg gattttgtct
    13681 cgattggacc aactattctc aacgcccatt ctccggacga aaaagtgcat attccggcag
    13741 tggagattta ttggaaactc ctaaccaaac tacttgaaca agcacctgag aaataaattt
    13801 aatcaaaaaa attatataat cttgccccgt aattaattga ataaaacctt aattaattac
    13861 ggggtagctc ttattttctc tgttttaaaa aatgtaccgt gaaagcagca attaataaga
    13921 caactgctgc acctaaataa agctcgttct ttttccagcc ggcgtctaat aaaatacctg
    13981 caagagttgg ggaaacaatt gagactaaac gccctacacc gatggcggtg cctacgcccg
    14041 tgctgcggaa atccggtgcg tatagggttg ggttaatggt ataaagcccg gtaatgcagc
    14101 cgttaattaa tccgccaacc aaaatggcta gtgttatcgc aagccctaaa ttgccggcaa
    14161 acacaaaggc aaccacagca gatgcagcca gaatactgaa tgcctgcaaa atatgttttg
    14221 ccgcccagcg actgactaaa aagccgaaaa ttaacgagcc gattgtacca ccaactgaaa
    14281 ttgccattcc cacagtttgg ctttgggttt taggcattcc ggcttcttct aatagtgccg
    14341 gagtccaaga gctgataaag taaaatgagg ctgaaattgc aataaaggca agccaaatta
    14401 gcaacgtatt gcggcgatat tcaggctcga ataaacgggt aattgccact ttgcttttac
    14461 tttttgcctc aatttcagga agttcccagc cgtttcctaa gcctaattta acggcaattt
    14521 gatttaaacg agctttagca ttcaccgggt gttttgaaat taaaaattcc accgattcgg
    14581 gcaatacaaa gaataataaa acgaataccg caccggttaa aatcgaccca acgaggaaaa
    14641 ccgcacgcca gccatattct ccttgcaata acaccgcaga cattccgccc agcattgcac
    14701 caatcccaaa gcctgaggca taaattgcaa tcgctaagcc acgccatttt tggctggagt
    14761 attcactcac tagcacatta gtgccggtta aaatggcggc tacgcctaaa ccggttaaaa
    14821 cacgccatac accaatttca aacgcagaag tagcgaaata agttaggagc attcctaacg
    14881 tggctaagcc tgtgccgatt aaaagcaacg ggcggcgacc aaatttatca gcaagtggtg
    14941 aaagaaagag tgagcctatc gccataccga ataaaccggc actcattaag ctgccgatgg
    15001 ctgcaccgtc taactgcaat tcttctcgga tgcttttggc ggtaaaggcg agtgccatta
    15061 aatcaaagcc gtccagcata ttaagtaaca ccgtaagccc aaccactaac cattggaatg
    15121 aagtcatttt gttatgagca attttttgac gaatatccat aagctccctc tgcatcaaaa
    15181 ttgataaaat tttatttaga gtgaactata gaaagttgat taaatataat caaactcaaa
    15241 atttacataa aatttatgaa agattttcat atttaatcaa aggagggaat ttatagcagg
    15301 aatggcgagg gagaaaatag atagggcgaa taattattca cccctagatt gaataggaga
    15361 aagctatttc ttctccgctt gctcagccgc tcttttgcgt ctgatttctt tcggatcagc
    15421 aattaacggg cgatagatct caattctgtc gccttcttcc accgtttcat tcagttttgc
    15481 cgggcggcta aaaataccga ctttgttttc acgcaagtca atttcggtat atttttccaa
    15541 cacgccggag agcaaaataa tagtttgaat ggtcgcgttt tgcggtactt gcagtttttt
    15601 cagaaaatat ttatcagggt aagcataaag cacctcaaca ttaattttct gttcttcact
    15661 tttttgttct tctggatttt gacgttgttc agacaccata cacctctttt gcacgttgtt
    15721 tgaaagcatt taccattttt aaagttaatt cgttgaacac cttgccgaac accattgcta
    15781 caattgggct ggaaaattca aaatttaatt gcagggcaat tttgcaactt tgctcatcaa
    15841 agggggtgaa tgtccaagct ccgtgtaaat ggcggaaagg cccttcaatt aatgccatct
    15901 cgattttata gggcggcgtc attttattgt gggtactgaa ggtttggcta atgcctaatt
    15961 tctgaatatg taactctgcc tttaattcat tttcgccggt gctaagggtt ttggtactca
    16021 cgcagccgga taaaaattcg gggtattttt catactgatt aacaagttga tacatttgct
    16081 cggcactata aggcacgagg gcagattgat taacgattgg cataatgtct cttttggaaa
    16141 ttaacaagcg gtcatatttt agcgtaaatt tgcaagccta taaagaaaca cttttgattt
    16201 gaagatagac ctcttgcccc ggttggattg ctaattcatt aaaagaccaa aggctgatcg
    16261 tactccaaat ttgctgattt tcgacttgaa ttgctaaatc aacccgctcc aaatgcgttt
    16321 caattttcca aacattgcct tttaacacat tacgaattga gctttgagtc ggtttttgta
    16381 agctgatcga aacctctttg ctggcaatag tcacccttag cttctcgcct atttgatggc
    16441 gagggaattg cttcacccat aatcgctgtg cgttcaagct caagccaagc atattatagt
    16501 ggggaagtgt ttcttccacc ggtaattcaa gcaggctaat tcgctgatga tttggctgcc
    16561 aagcggaaaa tgccgagctg tgccaaaccg ctgaaagggt atcaaatgcg gttactttgc
    16621 cgttttccaa cagcacaaga tgatctgcca atcgaactac ttcctctaaa ctgtggctga
    16681 catacagaat cggtatttca agccgagcgg ataatttatc taaatagtcc agtagctctt
    16741 gcttacgagg gagatcgagg gcagataatg gttcgtccat cagcaaaata ttcggtgagc
    16801 ttaataacgc ccgcccaatc gccacacgtt gtttttcgcc accggaaagg cttgccggat
    16861 agcgattaag caaaggggca atgcctaaca gttccacaat ttgcaaaaaa tccgcagaat
    16921 ccgaccgctt gtagccatat tttaagtttt tttccaccga ataatgaggg aaaaggcgat
    16981 gctcttgaaa cacatagcca atgttgcgtt tttcaggtgg taaattgatg ccgttttggc
    17041 tgtcaaacag cacctgaccg tttaattcga taaagccggc ttgagcggtt gaaagccccg
    17101 caattaaatt aatcaggctg gatttaccgg caccggaacg cccgaaaata gcggtgattc
    17161 ctttattcgg caaatcgaga ttgacattga gcgtcagatt gccgagttgc tgaagaagtt
    17221 taagtttgag catagcgttt ttgcctttct gccagccatt ccgaacagaa tagggcaatg
    17281 agtgaaatca gaatcgccac caagcacaaa cgggcggcag ccatttcgcc atcgggggtt
    17341 tcaataaagg tgaaaagggc ggcaggaatg gtttgggttt ggttgggaat gttggagaca
    17401 aaggtgatgg ttgccccaaa ctcccccaaa gagcgggcaa aacccagcac gccaccggca
    17461 ataatgcctg aaaaagagag cggtaaggtg agcgtgaaaa atacttttaa cggtgtcgcc
    17521 ccaagcgttc tggcggcttg ctccaattta ggatcgacgg cttcaaaagc taaacggata
    17581 gaacgcacca tcagcggaaa cgccatcacc attgaggcaa gcacagcccc tttccacgaa
    17641 aaactcaagc tgaaatgaaa ccattcccac aaatattgcc cgattacccc tcgttttgcc
    17701 atcgagatca gtaacaaata gccaatgaca accggcggta aaaccagagg gagatgcaca
    17761 ataccattga gtagattttt gccccaaaac tgtttgcggg caagcagcca cgccacccac
    17821 accgcaagcg gtaatgcaag cactacggca atggaggcaa cttttaggct taaaacaatc
    17881 gcattgagtt cttgtggggt gaaattaagc ataatctaaa actattcggt tataagctta
    17941 ccctcgcccc ttgtgggaga gggatagatt tttcttcgtt cagaagaaaa atcagggaga
    18001 gggggaatat cttaaacgct atttgccctc tctctgaaaa atatgtctga acgacatatt
    18061 tttctgtctc gatactcgct tcgctcgctc ctacagagcc gttggaaaag ccaacgttca
    18121 aacgcaagcg tttgtcccac aaggggagag agtaaattta atttacagga taaaaccctg
    18181 ctgcttcaaa tttcgctttt gctttcggag ttttgaggaa atctaaaaat gtggcggcag
    18241 aggctttttc acttactgcc gcagcagggt aaacaatttc gccgtagctt tccggtggga
    18301 aaacggaaac cactttgact ttattcgaca ctttggcgtc ggtggaatag acaatcccaa
    18361 gcggggattc gcctcgctcg acaaaagaaa gggcatctcg tacatttttt gccctcgcta
    18421 aacgaggttc aacacttccc cataattggt aatggctaag agcttttttg gcatatttgc
    18481 cggcaggaac gtggtttggg tctcccaccg ataaatagct gtttgctaat tctttggcaa
    18541 aatcgaccgc ttgtatcgct tttggctcga tgttgctctc ggttggtgca attaatacta
    18601 actcgtttct gactaaattg cggcgatatt taaccttctc aggttgtttt tcggcaacat
    18661 aatccatcca cttttggtcg gcggaaataa aaatatctgc cggtgcatct tgctcaatct
    18721 gctttgctaa taccgatgat gaagcgaagg agaacgtgat tttatcctgt ggataggctt
    18781 tttcaaaatc ctgctgaata tcttgtaaca catttgtcat tgaagcggca gcaaacacgg
    18841 taatctcttg tgaaaaggct gtgcctgata acgttaatgc cattgtaatt gctagttttt
    18901 ttaatgcttt cattttcata acctcacaat tagttaagaa cgcagcccaa cccctcgttg
    18961 aattaaggaa taggcaataa tatacaagcc gatgcaaagt gaagtaagca ctgcaaaagt
    19021 ataacctaac ggcacatcgc tgataccgag aaagccatat cttaagccgt taatcatata
    19081 aacgatcggg ttgaatttag acaccgtttg ccaaaattca ggcagtaatg aaatagagta
    19141 aaatacgccg cctaagtaag ttaagggagt aagcacaaag gttggaatca gccccacatc
    19201 atcaaaggag cgggcgaaca ccgcattaat tacgccacct aacgcaaagg caatgattgt
    19261 cagcaacagg gttaagacga taatcgcaac cgagtagatt tcaaacgaga caaacaccat
    19321 tgccacgcac attactaagg ttgcggctaa acctgcacgc aaaatactgc cggtgacata
    19381 accccaaata atattatggg tagagagtgg cgaaatcagc atctcttcaa aatctcgggt
    19441 aaatttactc aagaaaaatg aagaggcggt attcacataa gaggcggtga tggcggtcat
    19501 cataattaag ccgggtgcaa taaattgggt gtaacccacg ccattcattt ccccgattcg
    19561 cccaccaatc agcgtgccga agattaaaaa atagagcgtg gtagtaatca ccggtggcac
    19621 taaagtttgt cgccaaatcc gcaaaatgcg gcggcactct ttgtaagcta aggtataaaa
    19681 tccgattaaa ttcattttac tgagccatat tgataaatag ttcttccaag cggtttgatt
    19741 tgtttcgcat acttagcacc tcagcccctt gggcggaaat ttgcgaaaac agattggtta
    19801 gcccttgctg gcgttttaca ttcacttcaa gggtgttttc atcaagccat ttatgcgggt
    19861 aattttgaat cacaagcggt tgattttgct cagaattttg caaatctaac acaaatacct
    19921 cactttccaa tttggcaagc aaggctttca ttgaggtgtt ttcaattaat tcgccgtgtt
    19981 gaataatgcc gatgtgacgg catagggttt ccgcctcttc taaatagtgg gtagtgagaa
    20041 taatagttgt gccttgattg tttagatccc gcaagaaatc ccacaatgag cggcggagtt
    20101 caatatccac ccctgcggta ggttcatcta aaatcagtag ttttggattg tgcattaagg
    20161 ctcgggcaat catcacccga cgtttcattc caccagaaag ctcacgggtg aggtgggtgc
    20221 gtttttccca taggtcgagc aatttcagcc acttttcggc acgtactaag gcttcactgc
    20281 gaggaatgcc gtaaaacccg gcttgttgca ctagcacatc aatcactttt tcaaactgat
    20341 tgaaattaaa ctcttgtggc actaagccga tttgatgctt taattcgact ttttgggtgt
    20401 ctaaatcata gccaaacact ttcacgctgc cgctggtttt atttaccagt gagctgataa
    20461 tgccaatagt agtagattta cctgccccgt tatgcccaag tagggcatag aaatcacctt
    20521 gctcgaccac aagattaatg cctttaacgg cttttacacc tgttgggtag gtttttacca
    20581 aatcaattaa ttctaatgct ttcatttgtt ttactcagtt tccgccaccg ctcgaatggt
    20641 cgattcatca atttgtgcat caaaagcgtt gcgaagtaac attaattttt catcgttaag
    20701 cagagctgat tttgctgcct cagttaattg tttgaaaatg gctcgccgaa tttcaagcgg
    20761 agtttgctgt tcgctattgt caccaatggt caattcgtat gaatagcctt ttttggcaag
    20821 ggcagcttct aaatttttac gtgattccgg attatctaag tgagccatat tcggctttaa
    20881 caccaatgcg aggtgctgtt cattttgttc cgctaaatag ctatttaagg cgatctgacg
    20941 ggaaaatccg cctaaattga gtgagttggc gatgtcacac catttatcct gctgacaaga
    21001 taaatgaatg gtttttgcca ctaattcagg ggtgcgttct tgcaaaatcg cttgtttaat
    21061 atcagaaggt ttggcactgt ctgcttgatt ttctaattca ggattgagcc acgtccagcg
    21121 ataatcttca tcttccacac ttttaggggc ggattcggtt ttctttgaaa cttgcagtac
    21181 ggctttgtgc tgattttccg ccaagctgat aaaccgctct tgcaatgaag cggtctgatt
    21241 tgccgatttt tttgcaataa cttgaggggc ttttagctca ggcttttttt tttcggtttg
    21301 agctaaattt gcctgctgtg cttgtagctt tttgcgggct tcgagggctg ccattgccgg
    21361 tgaaagagtc gcaggagcag acggcgaaat agcggtatct actgcctgtg gcggttgagc
    21421 aatgctttca accgctgtgg tagtattggc aacagtaacc gttttttgct gattttgctc
    21481 aaaattttct cttagctggc taatttgatt ttgtggtttt gctgtcgtaa ccgctgaaac
    21541 cgaatggttt ggttgtgtaa ccttttccac tgcaactgtt gctatttttt ctgcctgttt
    21601 agggtggaaa gccaacgcac gcaaaatcgc catttcaaca ccggcacgtt gctccggtgc
    21661 aaaaggcaac tcttttttac tgttgaggat taattgatag aaaaattgca cgtcttcggg
    21721 cggaatttgt tttgccagaa aatgcactaa tgtttcttcg gttgtaggtg ttttcactaa
    21781 ttgcaacatt gcaattttgt gtaacacttc tgccacatcg cttaataatt gttgccaatc
    21841 aacgcctttc gctgcaattt gctcgatgat cgccattgct ttttcaccgt cggcttgggc
    21901 ggttgcttgc actaattcta gtggttgatt atcgtcaatc aaccctagca tttggctgac
    21961 tatcgcaagc gtaatattgg cattactcat tgcaatggct tgatcggtga ggctgagaga
    22021 gtctcggata ctaccttgtg ctgcttttgc caacttttct agtgccaaag gctcaaacgg
    22081 aatgttctct gcatttagaa taaattcaag atgacgggca atttgcgatt gctctaatgc
    22141 ccgcagatga aattgcatac agcgagagag gatcgtaatc ggcagttttt gcggatcagt
    22201 ggtagccagc aagaatttga catattcagg cggctcttca agggttttta gtaaggcgtt
    22261 gaaactatga cgagagagca tatgcacttc gtcaattaaa taaaccttaa aacgcccaac
    22321 ggtgggtttg tattgaacgt tatcaagtag ctcacgggtg tcttccactt tagtacgaga
    22381 agcagcatca atttcaatga gatcgataaa atgcccttct tcaatcgctt tacaattcgc
    22441 acattcaccg caaggctcgg cggtgatccc gtttacacag tttaagcctt tggcgaataa
    22501 acgggcaata gaggttttac ccacaccacg agtgcctgaa aacaggtaag cgtggtgcag
    22561 tctatcttct cttaagctgt tttcaagagc agataaaacg tgttgctgac caactacttc
    22621 cgagaagcgt tgcggtctcc atttacgggc taaaacttga taactcattg atttttctat
    22681 tcaatttgga aatcacaagc ggtcatcttt ccgaaaaatt ttgcaaaatt ttagcaaaaa
    22741 gccaccgctt gttccccatt aatgaccttc aaagctgact aggctaaagg atttaatccc
    22801 ttctttttcc aaacgctctt taccacctaa ctcaggcagc caaatcacaa aggcagcgtg
    22861 ttctacgtta ccacctaagc gacgaattag ttttgcagtt gcatcaatag taccaccggt
    22921 tgcgagtaaa tcgtcgatga ccaatacatt atcattagca ctcactgcgt ctttatgaat
    22981 ttctagcgtg tcttcaccat attcaagcgt gtaagattgc gaaatcactt cacgtggtaa
    23041 ttttttcggt ttacgaacca acacaaaagg cacgcctaac gctaatgcaa caggggcacc
    23101 aaaaataaac ccgcgagatt ctgtgccgac aattttagta atacctttgt ctttaaattc
    23161 tgcaacgatg gcatcaatgg tggcttggaa tgcagttggc acttctaaaa gtgaagtgat
    23221 atcacggaag ataatccctg ctttcggata atctggaatg gatttgatag aagatttaat
    23281 taaatctaat tggttcattg tttgacctac gtgtaataaa aaaataactg gaaaactcta
    23341 tagaaaaaca gcagaaattt caaggagaaa ttgataacct tacgtttgat agtacagctt
    23401 acttttcagt ttccttttcc ctatattata gataaatatc ttccctgttt cgtctggttt
    23461 taagcaaacg taaaatccaa acatactgct cggggttttt ctccacaaaa tattcaatta
    23521 ctcggttcat ttctcgggcg gattgaattg ggtctcctga aaattcaagc ggtggcaaaa
    23581 tttccatttc atattttgct ttttccgcat tataaatcga gaacattggg atcacttccg
    23641 catttgccac tcttgccatt ttatttaagc ccggcaaagt ggctttttcg gtggcaaaaa
    23701 agtcggcata cacacttagc tcttcgccga aatcttcatc gggcagaaaa taaccgacat
    23761 cacctttacg cacatcggct aaaaacgctt taatcccatt ttgacgagtg tgcattcgcc
    23821 cacctaaacg ctcacgggta atattccaca gccagtccac caacgcatta cggtgtggat
    23881 tgtacattga tgtcatcgga taaccgagcg agaacatcgc cacccccgcc atatcaatcg
    23941 accaagtatg tggcactaac ataatcacgt ttttaccgct ctctttggct ttcaccacat
    24001 tttcaaatcc ggtaaattca caccgttttt gtaaataaga ggccgggcgg attgcagttt
    24061 cgccaatcgc caacatggtt tgtgctaccg tgataaacat tttctcgacc acttcttcac
    24121 gctttttctc actccaatcg ggaaaacagt aacgcaaatt cactttagca cggtggtagc
    24181 cttttttatt tttctgcttg agatagcgat acgccatctt acccacaaaa gcggataatt
    24241 tatccctaat tcgcaccggc acataggcta aaatcactaa tcctaataca cctaaccata
    24301 cgccccaata tttcgggtgt agaaaagacc ataggaattt gtgcgtatag ccgtcttggg
    24361 cggtaattcg tgtcattcta taattcctca ttatttgtca ctttctttgc ttcgccaaag
    24421 aaagtaacca aagaaaggca accctacttc accgctatct tcgcttggtt tgaatttgct
    24481 taacgaaaaa attccaaact cgccgcttcg ctgctcaaac aaggaatttt tcctacaaat
    24541 tcaaagtcgc tcaggcggtt cagaagggaa cccggcagag catcattttg caaaattttt
    24601 caaaaaaatc accgcttgta tggtcggatc gggttccttt ctgagttgcc cgaacgactt
    24661 gaaattttta ggaaaataac tcgagcgaga gcgaagcgaa catcagggtt atttttcgtt
    24721 aagaaaattt caaccaagtg aggatagcaa caaagcaggg tgtgctttct tttggttact
    24781 tttctttgca caagcaaaga aaagtgacat aacacatcat ctcttatcaa taatattctt
    24841 caccgccaat atcaccccaa gcccaataaa agcccccggg ggtaaaattg ccagtagtaa
    24901 acctgaatca atatgcaata catcaatgcg tagggctttt gcccaatcgc caaggagtag
    24961 atctaagcct gaaaacagcg tgccgttgcc aataatttca cgcaacgcgc ctagcaccac
    25021 aaggctaaaa gtcattccca gccccattga aaagccgtca aaagccgaga atttgacctc
    25081 attttttgag gcataggctt ctgctcgtcc aatcacgata cagttggtta caatcagcgg
    25141 aataaaaatc ccgagcgatt ggtaaacagg gaaagcaaaa gcattcatta aaagctgtac
    25201 tgccgttacc accgttgcaa ttaccatcac ataaatcgga atccgaatat cgtgtggagt
    25261 gattttccga aacagggaaa ccgtgatatt ggtgcaaatt agcacaaaca ttgttgccaa
    25321 gcctagtcca agtgcattgg ttacgctgtt tgaaaccgcc agtagtgggc aaagccctaa
    25381 taattgcacc aaagcaccat tatttttcca cacaccatca gcgaaaatcg ttttccaaac
    25441 gctggattgt ttcggggcaa cgtcaatatt cgtcacaggg atttgttgtt ctctttccat
    25501 ttctccccct atttgcacgc tttaaattga ttaaacattg tcgggttttg agccagctct
    25561 gtcaccaccc atttcgcact ttggcggata tgattgacca ccgcacgagg tgtaatagtt
    25621 gcaccggtaa attggtcgaa cttgccgcca tcttttttta cattccaatc cgcttcgttt
    25681 tctaaactaa aaagttgatt ggtaaaagat aagatccaat ccgacactcg ggtctcaatt
    25741 ttatctccca gccccggagt ttctttatgc tcaagggtgc gaacgcctaa aatgttgccg
    25801 ttcggctcaa tccccatcaa tagcacaata tcgccggaat agccgtccgg agccgttcct
    25861 tgaactaggt aagcggtcag attttccgat ttttttgcaa cataaatacg gttcaaataa
    25921 ggggcatttt cgagctgaac cagtttgcaa gtcgccaaca catcattatc aaaatggctt
    25981 ttcggcacaa cctcttccaa taatttgcgt tgttgcattg cggttacatc atcaatcttg
    26041 cctttggtaa gcaggtacac accggttgaa acagcggtgc aaagcaaggc aataaagcct
    26101 aaaattaagg cgtaatttaa ggtaattttt gaggtgttca ttatctgtta tccaattttg
    26161 tgccgtataa tctcggctgc gtataatggt cgattaacgg tacgcaaatg tttgccaata
    26221 acactgaaaa tgcaatcgca tcagggtaat ttccgtagta gcggattaga taaaccaata
    26281 atcccaccaa gccaccgaaa atcagttttc ctcttggggt aatggaggct gttaccgggt
    26341 cggtcgcaat aaaaaatgcc ccgaacatca tcgcaccgct aaagaaatgg ctcacaaaat
    26401 gcaagtgcga ttgttggcta aacagctcgg tgaatccact aaaaaaggca aaagttgcca
    26461 acattgctac cggaatttgc caatggataa tccgtttata aatcaacacc aaaccgccga
    26521 ataagaacgc aaggttcagt tgtagccaac cggtcgcaaa cagcccgttg aaaatcggcg
    26581 tattcattac cgcgtcaaaa cctgattttg caaggctgct tttcatacta tcaagcgggg
    26641 tagcttgtgc aatgccatca acactattaa ctagctgatg aatactaaaa ccatcgctag
    26701 ttaccccgta aaaaatcaac gaaatcgcat cacgaaatgt cggtggttca cttaataact
    26761 caatcggcac tatccacgcc gtcatttgca ccgggaaaga aaccaacaac aacgcatagc
    26821 caaccattgc cgggttaaat agattctgcc ccaagccacc ataactgtgt tttgccaata
    26881 ataacgccac aatcacgcca atcaacacaa gccaataagg agcataaggc ggaatcgaca
    26941 tcgccaaaat caaggcggtt aaaatacctg ataagtcagc caaatagaaa gcggttgttt
    27001 ttttgcgtaa ttttgcaatc ccgatttcaa tcacaatcgc aaggctaacg gcaagcaacg
    27061 tttgaatcag cacaccatag ccgaaataat aaagctgaac cccaaaagca ggtaagatca
    27121 tcgcaatcac ccacagcata aaattcgcag tgagattgct cgaatgagtg tggggggaac
    27181 tgaccatttt aaacatctat aaaacctttt tagtaaaaaa ttgcaaaaaa atgaccgctt
    27241 attaaagtct cggcaaattc aaattaaagc gtattgccag caggcggata ataattacgg
    27301 tgaacacggt ggcaaggtaa acccaatcgc tttcgagccc aactgagttt gcagccacaa
    27361 aaatcacacc accgataagg ctggcggtca catacacttc tttttgcaaa atgatcggca
    27421 cttcgttgcg taaaatatca cggattaagc caccaaaaca gcctgtcaca ccgcccagag
    27481 ccacacagat cagtgggtgc aagccgaaat ccaagccttt ttgcacgcca atcacggtaa
    27541 atacgccaag cccgatggca tcaaacacca gcaagccggt ttgccaactt ttgcgggaaa
    27601 tttggttgcg gaaaatagcg gtaatcacca ctgcaagggt aatcatcagc acatagatcg
    27661 gttgtttcat ccaaaacacc ggcgtggagc caatcatcat atcccttagc gtaccgcccc
    27721 cgaccccggt cacaaacgct aaaataaaca taccgaaaat gtccattttg tgctggcggg
    27781 cagcaattgc ccccgaaatc gcaaacacga tagtgccgat taagtcctga ataaacagga
    27841 acgaccacgg caatgccgga attagctcgc taaacattgt tgcttttctc ctgctgagcg
    27901 gcttttttcg ctttagctct ggcgatggcg gccgcaacgg cggctttgcg tgggtcttcg
    27961 ctttccgttt tgggcgaatg gttattcgcc cctacagatt tatccgatgt agtttcgttc
    28021 attgattgta ggggcaaatc attatttacc ccaatcacag cattggcaat atcttccggt
    28081 ttttcgaccg cttgttgagc ttgttttttc gctttagctc tagcgatagc tgctgcaacg
    28141 gcggctttgc gtgggtcttc gctttctgtt tcgggcgaat ggttattcgc ccctacagat
    28201 ttatccgata ccgtttcgtt cattgattgt aggggcaaat cattatttac cccaatcacg
    28261 gcattggtaa tattttctga tttttcgacc gcttgctgtg cctgtttttt ggctttggct
    28321 cgggcaaggg cagccgcaac ggcggctttg cgtgggtctt cgctttctgc ttcgggcgaa
    28381 tagttattcg cccctacaga tttatccgat gcagtttcgt tcattgattg taggggcaaa
    28441 tcattatttg ccccaatcaa ggcatttgta acattttccg atttttcgac cgcttgttct
    28501 gcctgtttgg ctaaacgtct ggctctgcgt tgtgccatta aatcgctgtt atcaggctcg
    28561 ccgttaggtt ttacctgtac ttctgccggt atttcttgga ctgtttcaac cgcttttttc
    28621 gctttcaggc gttcaagagc cgcttttaca ggatcaacac cacctgcatt tgccacttct
    28681 tcacggcgtt tttccgccgc ttggctaatg cgagcagttc tggcatctcg ttctttttgt
    28741 aagcgagctt ctctcgcctc aaagcggatt ttagcctctt ccgctttgcg ggctttttcc
    28801 tcaatttcct cgatttttgc tttttcttgg cggaaatatt ggattaacgg aatgtagctt
    28861 gggcaaacgt aagcacaaac gccacactca atgcaggcat ctaaatggta ttctttcgat
    28921 ttatcgtgat cttctgcacg ggcaaaccaa taaagttgct gtggcaataa ccccaccggg
    28981 caagcatcgg aacagcttga acagcggata caactacgct ccggttcagg tggtgcatat
    29041 tcaaaatgat caggggcaat caagcagttc gcagttttgg taatcggggc ttgcaatgca
    29101 ggcaaaataa agcccatcat cggtccgcct aaaaacaccg gaaagcgttt atcggcttga
    29161 taatctacct gctccaacaa gtgctgaatt ggcgtgccta atcttgccca cacgttcccc
    29221 ttgttgcgaa ttttatcgcc tgtcagcgtc actacccgtt caatcaaagg ttcatcatca
    29281 ataattgccc gtttaaccgc aaacgccgtg ccgacattgt gcatcacaat gcccatttca
    29341 atagtgcgtt tgccagccgg aatctcaagt ccggtcaaca cttgaatcag ctgatcagaa
    29401 gctccggagg gatatttagt cgggatcgcc cgcacaaaca tatcgttcga gccttttaaa
    29461 gcggtttcta aagcttgaat agcgtgcggt ttgttgtctt caatcgcaat caccacttct
    29521 tccgggcgaa gcacataacg cagaatgcga ataccttcga taagatccgc agtgaaatct
    29581 tgcattaagc gatcgtcaca ggtgatgtaa ggctcacatt ctgccccgtt aatcaccagt
    29641 aatttacagc gtttatccgc caaacttaat tttgttgcgg ttgggaatac cgcaccgcct
    29701 aaaccggcaa tgccatattg gtaaactttt tggataattt gatcgcttgt taggcttaaa
    29761 aaatcctcaa tcggctggcg ttccacccat ttatctttac catcggtttc aataatcacc
    29821 gttatttcgg gcaaacctga ggcgtgagcg gaaacataag gctcaatccc aatcacccgc
    29881 ccggaggttg ggctgtgtac gggcagttga cggaagtgat cgcctttcgt gagcggctgc
    29941 cctttcaata ccacatcgcc gactttgact aataattccc ctgcccagcc agaatgttga
    30001 accagtggca catagaaaaa cttcggtaaa tttaaggtgc gaattggctt gctgtttgat
    30061 tgggatttat tttccatcgg gtgaataccg cccgggaaat cccacagttt acccgtttct
    30121 ttattttggc ggattcgctc aatcactaaa ttaggattgc tcatttttgc ctccgccggt
    30181 aactaatttt ctctgcaatt cagtggtatt cacaatcgga ataattaaat cttggtcaaa
    30241 tttccagttc caagattggg ttgtcggttt aattggtcgc atttcaatac agttggtcgg
    30301 gcaaggggcg acacaaagtt cacagccggt gcaaagatcc ggaataatgg tgtgcattgc
    30361 tttattcgtg ccgataatcg catcaaccgg gcaagcctga atgcacttag tacagccaat
    30421 gcagagatct tcaatcacaa aagccacttt catctcaggc tctaccatac cttccattgg
    30481 cggcacttct accccaagca attcggcaag attcaccact aaaggttgtc cacccggcac
    30541 acatttagtg atcacatcac cattagcaat cgcctcagca taaggcttac agcccgggta
    30601 gccacattga ccgcattggc tttgtggtaa aagggcatca atttgatcga caatcgggtc
    30661 ggattcaact ttcagtttaa tcgaggaata gcctaaaacc gccccgaaaa tcagtgcgat
    30721 cacagcaata gcgataagaa tataagcgat aattggcaaa tcaagcatta gagtttcacc
    30781 aatcctgtaa agcccataaa ggcaagcgac atcaagcctg cggtaatcag agcaattgat
    30841 gctccttgga acggacgagg cacatcagcg gcagccaaac gctcacgcag agccgcaaat
    30901 aaaactaaca ccagcgagaa accggcagcg gctccaaaac cataaagaac agattccacc
    30961 aagttattgg ctaaattcac gtttaataat gccaccccta atacggcaca gttggtggta
    31021 atcagcggta aatagatccc aagcaagcga taaagtgtcg ggctggtttt atgcacaatc
    31081 atttccgtta attgcacgat caccgcaatc actaaaataa acaccaaggt acgcaaaaat
    31141 tgagcgttta acggctcaag caaataggtt tccatcaaat aagcagagag cgaggcaacc
    31201 gtcagcacaa aggttgtcgc caaccccata ccaatagcag tttccacctt tttcgacact
    31261 cccataaacg gacaaagccc tagaaattta actaagacaa agttattaat aagggcggtg
    31321 ctgatgacca acagaatata atcgaccata aattccacaa aattaaatat acaagcggtc
    31381 aaaaacgcat tttattttgc aaaaaacaga gaaaatctga ccgtttgtta aagcatctca
    31441 ttaaaaataa acgttatatt atctcttgtt ttcggctcat tcacaataag aaatcagagg
    31501 tacttctagt aaacatacca tatcaaatat gatcttgtta cctttgtttc tatctctggt
    31561 agaatggaga atgtggggac taagctatac agcatgaaag gtgaaaggag cttgattttc
    31621 taagttaatc ctaaaaagtc tcactccttt gatatagagg atattagaat aacaaccaat
    31681 taattactcg ataaataaac ataataatcc caaatataat gaaaacataa taaatctttt
    31741 tccataaagg gagttttacc tcttcttcag aatattcttc atcatccttt tcagacaact
    31801 ctataagatt atagaattca tcaagcattt tttctttgaa tttatatcct cgatttattt
    31861 taagttcatt atgagacgct gccgaaagtg tttggaaaag aaacgggttt tccagtacgt
    31921 ttggaaagtt ctcttgaaca taagctttag attgagataa tttgttaaaa gtatggaaac
    31981 aaccaacata cataataagt tgttttgttg gcatttcttt tacccaagat tcaagttcat
    32041 cacactgtgc tataacctca tcaacttgtt ctttcccaaa ttgttttaca gcatctttat
    32101 agtgatcact atttttcttt ttgaaataag gtggtaataa tccagccatt tttactccac
    32161 attggttaga gataaattaa gtgttatctt ttttgctaaa ccatttattg gctacaaaac
    32221 caaccccagc agttacagcc gcaccaagta acattgaact gagcgatgaa ctttcttgtg
    32281 gttgagattg cttttccttg acagattttg ttgtagcatt agcttttgct ggacgtgcta
    32341 catatgtctc agaccctcta acacaagaaa taatttttgc gtatggatgg ctcgctttaa
    32401 aacgattttt agcatctata atatttcttt cagaaacagc accttgccca ttcttattat
    32461 atctggtatc tg
//
LOCUS       DANJWC010000010        27604 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.010, whole genome shotgun sequence.
ACCESSION   DANJWC010000010 DANJWC010000000
VERSION     DANJWC010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 27604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27604
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.010"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            1..585
                     /locus_tag="PWX02_000313"
     CDS             1..585
                     /locus_tag="PWX02_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HDL1728367.1"
                     /translation="MLKKLLIIDNHDSFTFNLVDLLRKLDVPAKVVLVEELDLNEVEH
                     FSHILISPGPDVPRAYLQTFAMLERYHQTKSILGVCLGHQTICEFFGGKLYNLNDVRH
                     GQQRTLTQIEPNPIFKGLPKQLKVGLYHSWGILQNSLQNTPLVPTAVCDQNVLMAFKH
                     QHLPIFGVQFHPESFITEFGQQMLANWLEITQDF"
     gene            682..1764
                     /gene="prfA"
                     /locus_tag="PWX02_000314"
     CDS             682..1764
                     /gene="prfA"
                     /locus_tag="PWX02_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDL1728368.1"
                     /translation="MKDSIINKLESLSERHEELQALLGDASIISDQDKFRAYSKEYSQ
                     LEEVVGTFNRWKKLNNDIEEAQLLLDDPDMKDMAAEEIAENKAEIENLEQHLQILLLP
                     KDPNDEYNAFLEIRAGTGGDEAGIFAGDLYRMYSRYCESKRWRIEEMSANESEQGGYK
                     EIIVKISGEGVYGQLKFESGGHRVQRVPKTESQGRIHTSACTVAVMPELPESEMPEIN
                     PSDLRIDTYRSSGAGGQHVNTTDSAVRITHIPTGIVVECQDERSQHKNKAKALAVLAS
                     RIVQVEQERQAAEQADTRRNLLGSGDRSDKIRTYNYPQGRVTDHRINLTVYRLDEVMN
                     GKIDELIQPIITEYQADQLAALSDQN"
     gene            1852..3105
                     /gene="prmC"
                     /locus_tag="PWX02_000315"
     CDS             1852..3105
                     /gene="prmC"
                     /locus_tag="PWX02_000315"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL1728369.1"
                     /translation="MQTDNVTLSEWQFGEKPNTLANLVLAGIKTATCSGYCFYKLDNE
                     PLPQKGERHIVLDSQNCPVVMIELTCIERKKWFEIDEKFALLEGADSFSEWERTHRNF
                     FNAQLERAIDEQFELVCEQFHTVNIFSKTYAQWFRFAEQTLLENAEQDPFLNAKFDAN
                     LLLQAVTKRSKSAIFAFSETVLSERELVELAEKLARRAKGEPMAYILGYREFWSLPLK
                     VSPATLIPRPDTERLVELALEYANKRLENQKMLQILDLGTGTGAIALALASELGEKAQ
                     IIGVDFQNEAVQLAEENRQHLGLNQVTFLQSDWFSSLANQQFDLIVSNPPYIDEADEN
                     LTVGDVRFEPLTALVADNKGLSDLQKIIQNAPLYLKPQGALMLEHSWQQGNAVRDLFD
                     LNLWEQVETAQDYAGNDRVTKAILK"
     gene            3114..3980
                     /locus_tag="PWX02_000316"
     CDS             3114..3980
                     /locus_tag="PWX02_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB1 family protein"
                     /protein_id="HDL1728370.1"
                     /translation="MDDIIEELLKELKDPKVTISETELKKFLYREILRLTTLIDRNVS
                     EQQVFGQMSALVKKARYNVNGEGDEERINQLLDLVYREWGFHCYYEDYFHTENLLLNQ
                     VIRKRRGMPVSLGAVVLYLAAVLDLPIYPVNFPTQLVLRAEMRQPNGATKVRFINPWN
                     GEFLTIEMLNKWLEGEMGFGVEVTPDLLRRAEPQELLERVETVFKMALTREGKYEETL
                     RLIEYRLAFSPEDPYEIRDRGMVLASMDCYQAALEDINYFIDQCPEDPSAEILKMEVK
                     GLQQKSQESLVH"
     gene            4071..4925
                     /gene="kdsA"
                     /locus_tag="PWX02_000317"
     CDS             4071..4925
                     /gene="kdsA"
                     /locus_tag="PWX02_000317"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HDL1728371.1"
                     /translation="MTDKIVKVGNIEVANHKPFTLFGGMNVLESRDMALRVCEQYVEV
                     TQKLNVPYVFKASFDKANRSSIHSYRGPGMEEGLKIFQELKQTFGVNIITDVHEIYQC
                     KPVAEVVDVIQLPAFLARQTDLVEAMARTGAVINVKKPQFLSPGQMGNIVEKIAECGN
                     DKVILCDRGTNFGYDNLVVDMLGFGIMKKVSKGCPVIFDVTHSLQCRDPFGAASGGRR
                     DQVTELARSGMAIGVAGLFLEAHPDPNSAKCDGPSALPLSKLEAFVSQMKAIDELVKS
                     FEEIDTSK"
     gene            5148..6095
                     /locus_tag="PWX02_000318"
     CDS             5148..6095
                     /locus_tag="PWX02_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HDL1728372.1"
                     /translation="MLNIVFLDRTGIPATHEIPRPNFPHTWTEYATTSADQTFERVKE
                     ADIIITSKVLLQRDLLAKLPKLKLIAITATGTNNVDLAAAKELGIAVKNVTGYSTVTV
                     PEHVIGMIYSLKHRLADYHRDLITSDRWATCGQFCYVDYPIHDVRGSTLGIFGKGNIG
                     AEVARLAQAVGMQVIFAEHQGATEIREGYTAFEEVFKQADIISLHCPLTEQTQNLINA
                     ETLALMKPTAYIINTGRGPLIDEQALLEALEQGKIAGAALDVLVKEPPEKDNPLMLAA
                     KRLPNLLITPHVAWASDSAVTTLVNKVTQNMEEFVETGK"
     gene            6379..7551
                     /locus_tag="PWX02_000319"
     CDS             6379..7551
                     /locus_tag="PWX02_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HDL1728373.1"
                     /translation="MNLTPFFIAFRYFRSKSADRFGRLVTNLASLGIVLGVMALVIVL
                     SVMNGLENMQKTNLLSTLPHAIITPQEGHFAKGEKLALPEFAKQSVVINRANVIIQSS
                     QGINAGQLIGVENATDDPLLVAENIPALLPSGEFKVVIGSRLANKLNVTVGDKLRLMI
                     TENSQYTPLGRVPVQRLFEISGVYQSNREASEFTLFANLADVGRLLRISEDQVQGVRL
                     YLQDPFQVTELSQYFDAERYQISDWREQKGEFFQAVKMEKNMMGLLISLIIVVAISNI
                     VTSLSLMVVDKQGEIAILQTQGLTKKQVMQIFVFQGAIVGVIGSIIGGLLGVLIAYNL
                     DQIIQQLNPSIHLPTLISGSQIAVIVGVSMLLSLLCTLYPAYRASKIEPAQALRYE"
     gene            7575..8258
                     /gene="lolD"
                     /locus_tag="PWX02_000320"
     CDS             7575..8258
                     /gene="lolD"
                     /locus_tag="PWX02_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HDL1728374.1"
                     /translation="MTELLRCEHISKFYDEGEQKVQVLKEVSFSMNEGELVAIVGSSG
                     SGKSTLLHTLGGLDQPSTGEVWIRGQSLQQLSSDKLALLRNQNLGFVYQFHHLMADFT
                     ALENVMMPMLIGKQNKTEAVNRAEKMLQAVGLAHRMTHRPSALSGGERQRVAIARALV
                     NNPALVLADEPTGNLDQKTTESIFELIQQLNQEQNIAFLLVTHDLNLANKLSRSLVMR
                     DGVLSEGSL"
     gene            8255..9520
                     /gene="lolE"
                     /locus_tag="PWX02_000321"
     CDS             8255..9520
                     /gene="lolE"
                     /locus_tag="PWX02_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HDL1728375.1"
                     /translation="MSTPFFISWRYQRGKQKNRLVSLIALFSNIGIALGVAVLIIGLS
                     AMNGFERELNNRVLSVVPHAELVSFQGNQPQPIFDSKKLEALVKKSQNVTASSPFVSF
                     TALIENGAQLKIAQVRGVDPKKQDQASQLSQFIPPEQWQAFNQQFQADEDGLILGAGI
                     AKALEVEAGDEVTLLIPQPTEDGKLAQPLRFNLNVTGILRLDGQLDHSYALIPLNKAQ
                     ELMEYQANEVSGLEISLANPFDVQQLEMPQLNGYTQPLYLNTWIDKFGYMYNDIQLIR
                     TVMYIAMVLVIGVACFNIISTLIMAVKDKQGDIAILRTLGANNGFIRRIFLWYGLISG
                     MKGALFGLILGVILSLNLTAIIKAIEAFFEVKLLSDGVYFVDFLPSELHWQDVVYVLA
                     ATIILSLFASLYPANRAAKLEPAKVLSGH"
     gene            complement(9595..11526)
                     /gene="thrS"
                     /locus_tag="PWX02_000322"
     CDS             complement(9595..11526)
                     /gene="thrS"
                     /locus_tag="PWX02_000322"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDL1728376.1"
                     /translation="MPIITLPDGSQRSFDNPVSVMEVAQSIGAGLAKATIAGRVNGER
                     RDASDIISQDSALEIITAKDEDGLEIIRHSTAHLLGHAIKQLFPDVKMAIGPTIENGF
                     YYDIDLDRSLTQEDLDALEKRMLELAKTNYDVVKRPVSWQEARDTFEKRGEPYKMAIL
                     DENIERTATPALYHHEEYIDMCRGPHVPNMRFCHHFKLQKVAGAYWRGDSKNKMLQRI
                     YGTAWADKKQLAEYLQRLEEAAKRDHRRIGKALDLYHMQEEAPGMVFWHNDGWTIFRE
                     LETFVRTKLKEYDYQEVKGPFMMDRVLWEKTGHWQNYADLMFTTQSENREYAIKPMNC
                     PGHVQIFNQGLKSYRDLPIRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IESEVTSCIKMVYDIYSTFGFTNIAVKLSTRPENRIGDDAMWDRAEEGLANALRNNGL
                     EYEIQEGEGAFYGPKIEFALRDCLGREWQCGTVQLDFALPGRLEASYVAEDNGRRTPV
                     MIHRAILGSIERFIGIITEEYAGFFPTWLAPTQAVVMNITDSQADYVQQVVKQLSDAG
                     IRVKADLRNEKVGFKVREHTLRRVPYMLVCGDKEIAEGKVSVRTRKGADLGTYLVSDL
                     VEILKNQIKARELKLLGEE"
     gene            complement(11693..13042)
                     /locus_tag="PWX02_000323"
     CDS             complement(11693..13042)
                     /locus_tag="PWX02_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1728377.1"
                     /translation="MNSTKFYSLYSGIFFLFFGYGLFLNSAGIKLAEMGITDIIIGLL
                     NAAFFIGAALSTIFAHRIVSSVGHIRSFSVFCAVFAIAALGHIMIDTLWIWGVLRIIL
                     GFCHYSLLLLVESWLSEKTNVDTRGKVLATYNIIFYLAFILGVSLLSLELSSRNIFIL
                     STILVVMSMIPIALTKMVQPDIPARERISIPKLFAISPLALATSFISGMLVNGFFTMA
                     SVFLLKFSFSLSEISLYLMISMIGGFISQMPMAILSNRIGRRNAILACSVIATLTALA
                     GLFAIFQENLTAWGQYIIAFFFGCSLFSLYALSIARANDELPNNMNTVEVSRGLLFCY
                     GVGALVAPPLIGFAMSLSAEYGFYALFGLFSLSLFICAFRTKSIPKAERAEMQYATPI
                     ATASPLEEIEIVEDALIPFDEEAVQEYQAQLEILEQDPQTENENIELDEEAAENSKV"
     gene            complement(13246..13812)
                     /locus_tag="PWX02_000324"
     CDS             complement(13246..13812)
                     /locus_tag="PWX02_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005711111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDL1728378.1"
                     /translation="MFTTQLIQTKRWLEANSITTFWLKTFIGATLLAIFAQISVPMYP
                     VPITGQTLALTVVGFAMGRKAATAAVLLYLFEGAIGLPVFAGGASGFHHFFGPTGGYL
                     LGYIPTAYFLGYYSDKGVLNSFWKSLFVALIASVITFAFGLAQLSFFVPEGTVLQAGL
                     YPFILGGVLKALLASVLVSPSYKFFSKL"
     gene            13893..14651
                     /locus_tag="PWX02_000325"
     CDS             13893..14651
                     /locus_tag="PWX02_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--protein ligase"
                     /protein_id="HDL1728379.1"
                     /translation="MVKPVYIYCDEGSSEIGVQSLLLAVKQHLQRPAYLINAKQIIQS
                     KFSDGCLLIMPGGADLPYCKKLNGEGNRRIRQFIEQGGAYLGICAGAYYGCAELNFVG
                     RDYSVNGNRELALFNGIAKGSLPQFTNGQLYDKNIGSKAIVEVTLAEGGKMPFYYHGG
                     CCFEPNSTTVIYNPLAYYPDGSVAMIEGELGRGYYLLSGVHFELQAVVYWQKIVNCCE
                     NLDIKTQEEAICSNFNVNYGSQIWQHLKQRLNQA"
     gene            complement(14642..15436)
                     /locus_tag="PWX02_000326"
     CDS             complement(14642..15436)
                     /locus_tag="PWX02_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL1728380.1"
                     /translation="MRFFDTHTHLDYLAESLNLSIPELVENAEQHQVECILIVAVFAK
                     NFAKVTACAKQKPENLVYGLGLHPLYIQQHQQQDLILLENLLSQQDPQCTAIAEIGLE
                     KAVSEVSTPELWKKQCEFLEAQLSLAKKFNLPVNLHSRKSHDQLFVFLKKAQLTKTGV
                     VHGFAGSYEQAKRFVDLGYKIGVGGTITYERANKTREAIRKLPLESLLLETDSPDMPV
                     FGFQGQPNRPERLLVTFNSLCELRTESPNLIAETIWQNSERLFFYA"
     gene            complement(15497..17083)
                     /locus_tag="PWX02_000327"
     CDS             complement(15497..17083)
                     /locus_tag="PWX02_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDL1728381.1"
                     /translation="MLWFFFCVAVLVLGYFTYGKIIEKIFVINPKRSTPAYSMNDGVD
                     YMPMSKTKIWLIQLLNIAGTGPIFGPILGALYGPVAMLWIVIGCIFAGAVHDYFCGML
                     SIRNGGATMPNLAGKFLGRPVKLFINVMALILLLLVGVVFVASPAQLLSTITMDIAGA
                     TQSAMELGDVEAIKESVASGGVTVWGMDKATVISVWTLIIFAYYILATLLPVDKIIGR
                     IYPFFGALLLFMSVGMVYGLVSAHLSATDPIDFFRTVNADGQGLTWEKFTQNFQVKGD
                     VPIWPLLFLTISCGALSGFHATQTPLMARCAQNEKEGRFIFYGAMITEGVIALVWCAV
                     GLSFYENPQALQDAISAGSPSKVVYDSSLHFLGFIGGIFAVLGVVVLPITSGDTAFRA
                     ARLQLAEIFGLEQRTLVKRLYIAVPLFVLGFIVSKVDFSVLWRYFTWANQMTAMVMLW
                     TAAGYLYRYKKFHWVCSIPAWFITTVCATYLANNKIGFGLDYELSVYIGFAITVVCVI
                     CFFAFLKPSEARDEDAYVTN"
     gene            complement(17269..19122)
                     /gene="dxs"
                     /locus_tag="PWX02_000328"
     CDS             complement(17269..19122)
                     /gene="dxs"
                     /locus_tag="PWX02_000328"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDL1728382.1"
                     /translation="MEKKYPLLSEINSPEDLRLLSKDQLQTVVDELRAYLLESVSQTS
                     GHLASGLGVVELTVALHYVYQTPFDQLIWDVGHQAYPHKILTGRRDQMHTIRQKDGIH
                     PFPWREESPYDVLSVGHSSTSISAGLGIAVAAEKENAGRKTVCVIGDGAITAGMAFEA
                     LNHAGALHTDMLVILNDNEMSISENVGALNNHLARIFSGSLYTSVRDGSKKILDKVPT
                     IKNFMKKSEEHMKGVIFSPESTLFEELGFNYIGPIDGHNIDELVKTLSNMRELKGPQF
                     LHIRTKKGKGYTPAENDPIGYHGVPKFDPTAAELPKSKAAPTYSNIFGDWLCEMAERD
                     PKIIGITPAMREGSGMVEFSKRFPEQYFDVAIAEQHAVTFGAGLAIAGYKPVVAIYSS
                     FLQRAYDQLIHDVAIQNLPVIFAIDRAGIVGADGQTHQGAFDLSFMRCIPNMTIMAPS
                     DENEMRQMLYTAYTMNTPSAIRYPRGNAKGVKLEPMQGLEVGKANIIREGKKVAILNF
                     GALLDEAKIVAEKHNYTLVDMRFIKPLDEALLQKVADSHELLVTLEENAIQGGAGSFV
                     NEYLQNIGKIKPLVMLGIPDFFVPQSTQAEAYADLGLDAQGIEAKIQKFDI"
     gene            complement(19167..19979)
                     /gene="suhB"
                     /locus_tag="PWX02_000329"
     CDS             complement(19167..19979)
                     /gene="suhB"
                     /locus_tag="PWX02_000329"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012621556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HDL1728383.1"
                     /translation="MNPMLNIAIRAARKAGNIIAKGYEQSPQDTQVLQKNTNDYVTEI
                     DKAAEAAIIDVIRKSYPEHSIVTEESGVLNGSNENVQWVIDPLDGTTNFVKRLPHFAV
                     SIAVRENGRTTVGVVYDPIRNELFTAVRGEGAKLNEFRLRIDAERRDLKGTVIATGFP
                     FKVIRHRDAHLNMLEGLMNNGVADFRRTGSAALDLAYVAAGRVDGFFEIGLKPWDFAA
                     GELIAREAGAIVTNFVGGTDYLKTGNLVVGNGRVVKEILNSIQPTLTEELKA"
     gene            20160..21194
                     /locus_tag="PWX02_000330"
     CDS             20160..21194
                     /locus_tag="PWX02_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA methyltransferase"
                     /protein_id="HDL1728384.1"
                     /translation="MQFKENNQPRFKHREERRSDERSPRFSDKGKMRGNEGKSDRPHK
                     LHFKRDEAYSRSEQIYPAEGKKASGEGSVQIWVKGGNSSVKEKKNGPLSPRAPEKIKK
                     NRLEEMKVYGENACISLFQQRPGSIVRLWATVEGAKKLGDLMSYLAQHKKAYHVIDRE
                     EMERVTGTEHHGDMCLLVKKTASFVLEGYLQVPKKQDCLVLLDGVNNAQNVGGIIRTC
                     AFYGVKGIITENVDSLNSSSAARVAEGGLEFVRALETKNKHIALVQLRQAGYQIVHLT
                     RHKQAPSLTKIKLAEKVVFVLSEIPASDVAYAEDTTIQLSFNNPLNSGLNVAVNAGIL
                     LNQWYQTHIL"
     gene            21204..22088
                     /locus_tag="PWX02_000331"
     CDS             21204..22088
                     /locus_tag="PWX02_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HDL1728385.1"
                     /translation="MNQQKPFEVIAIVGTPRHDSAFETHLAVYNWLKDRDYQILVETE
                     VATQLQLKESYSLDQIGQRAELVIVIGGDGNMLGIARALAKYRVPLIGINRGNLGFLT
                     DIAPQTAFEQLYSCLVKNEYVIEERFLLEAFIERNGKIIAANNALNEIVVHPTQVARI
                     IEFEVYIDGKFAFSQRADGLIVATPTGSTAYSLSAGGPILTPNMNAMALVPMHPHTLS
                     SRPLVVDGDSQISLRFAKYNTPDLEVICDGQVNMHFTPEDRVLVRKSPDKLQLLHLKD
                     YNYFTVLGSKLGWASKLF"
     gene            22227..23204
                     /locus_tag="PWX02_000332"
     CDS             22227..23204
                     /locus_tag="PWX02_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1852 domain-containing protein"
                     /protein_id="HDL1728386.1"
                     /translation="MTTNFKCQVHTIEFNENYQPEQSTRLTTNFANLARGENRKQNLQ
                     NAIAMINNRFNDLANWDNPNADRYSVKLDIVSLDIDVEGKGEFFPAIEWLKTYVIDYK
                     TGKTIDGIIGNNFSSFVRDYDFSVLLLDHNKDKPQFSVPEGYGELHGKLFKHLLASPE
                     YTAKYKKPPVICLSVATTRTYTRTTNVHPILGVEYLQDSYSLTDAYFAKMGLKVRYFM
                     PPKSVAPYAFYFDESADLLNDYTNLELISTIATMEAFQKIYRPEIYNANSVAGQVYKG
                     SLTYQDYSVTKVEYDREERTQLAIKQGKYTEEHFIKRYQAILEAWATEE"
     gene            23292..24323
                     /locus_tag="PWX02_000333"
     CDS             23292..24323
                     /locus_tag="PWX02_000333"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL1728387.1"
                     /translation="MSNILLPTSTAGSLPKPSWLAEPEKLWSPWKFEGEELLQAKRDA
                     LLVSLAEQIAAGIDIVSDGEQTRQHFVTTFIEHLEGVDFSKREIVRIRNRYDASVPSV
                     VGAVSRPKSVFVDDAKFLRAHTEKPIKWALPGPMTMIDTLYDGHYKSREKLAWEFAKI
                     LNEEAKELEAAGVDIIQFDEPAFNVFFDELNDWGVAALERAAEGLKCETAVHICYGYG
                     IKANTDWKQTLGNEWRQYEESFPKLQQSKIDIISLECQNSRVPMDLIELVRGKKVMLG
                     AIDVATNIIETPEQVADTLRKALQFVDADKLYPSTNCGMAPLSRQVAQGKLQALSQGA
                     AILRAEFAK"
     gene            24411..24632
                     /locus_tag="PWX02_000334"
     CDS             24411..24632
                     /locus_tag="PWX02_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728388.1"
                     /translation="MLNLFKKPIKKSSFEAWAKLMDDVAKVAILAIPVILYGEYSLLM
                     KFTHSITLFAVVYFLLATARLFRETAEEM"
     gene            24908..26170
                     /locus_tag="PWX02_000335"
     CDS             24908..26170
                     /locus_tag="PWX02_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDL1728389.1"
                     /translation="MLKRDMNIADYDAVLWQAIQDEDRRQEEHIELIASENYASPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKALFGADYANVQPHSGSQAN
                     AAVYMALLNPGDTILGMSLAHGGHLTHGASVSFSGKIYKAEQYGITDEGLIDYEALRK
                     QAQEVKPKMIVAGFSAYSQVMDWAKMREIADEVGAYLFVDMAHVAGLIAAGVYPNPLP
                     HAHVVTTTTHKTLAGPRGGLILSASGDEEMYKKLQSAVFPAGQGGPLMHVIAAKAVCF
                     KEALEPEFKAYQQQVVKNAKAMVEVFKQRGFDVVSNGTENHLFLVSFVKQGLTGKAAD
                     AALGAANITVNKNSVPNDPQKPFVTSGIRVGTPSVTRRGFKEAEVTALAGWMCDVLDS
                     IGKDNHEQVIAETKAKVLDICKRLPVYA"
     gene            complement(26318..27373)
                     /locus_tag="PWX02_000336"
     CDS             complement(26318..27373)
                     /locus_tag="PWX02_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL1728390.1"
                     /translation="MGRPRKQQNRYLPENLRPKTVTRKSGKTVIYWLYRTHGKTEISL
                     GTNRNEAFIKAAQLNIERESQTARITFGLVAKRYRDEVIPQKKASTAQTDLCKIKNLL
                     LFFNDAPLEEITPQHIREYMTWRKDTPGSANNEFSMFSHIWQYAREWGYTNLPSPNIG
                     MKRYPIRKREIYVENNIFQMVYEVADQDMRDLMDLAYITGQRPVDLVNIRKEHIFDGY
                     LHIVQQKTQAKLRIEIVGKLADIIQDRLEKATGTHLFSTKKGEKLTARKLGKRFTYIR
                     EQAVKNNPEYANEIMAFQFRDLRAKSGTDKALSLGEEAARQQLGHTSVQMTKTYIRKA
                     PIVTPIKESVPSSKHQS"
     gene            complement(27333..>27604)
                     /locus_tag="PWX02_000337"
     CDS             complement(27333..>27604)
                     /locus_tag="PWX02_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HDL1728391.1"
                     /translation="QPTEFLTKDELADLTGYRQKKRQIEWLRERHYIIQDENKYKPLV
                     LYKDVYGHPRQQYPEAQSTGKWKSPAYLMGVKNGKTTQTTESLLT"
BASE COUNT         8237 a         5602 c         6010 g         7755 t
ORIGIN      
        1 atgctaaaaa aactattaat catcgacaac cacgactctt ttaccttcaa cttggtggat
       61 ttactccgta agcttgatgt gccggcgaaa gtggtgctgg ttgaagagct tgacctcaat
      121 gaggtggagc attttagcca tattttgatt tcgcccggcc ctgatgtgcc gagagcctat
      181 ctacaaacct ttgcgatgtt ggagcgttat catcaaacca aatcaattct aggcgtttgc
      241 ttggggcatc aaacgatttg cgagtttttt ggcggcaagt tgtataacct aaacgatgtt
      301 agacacgggc agcaacgcac gctcactcaa attgagccta accccatatt taaaggtttg
      361 cctaagcagt tgaaagttgg gttgtatcac tcttggggca ttttgcaaaa ttcgttgcaa
      421 aatacaccgc ttgtaccaac cgcagtttgc gatcaaaatg tgttaatggc gtttaaacac
      481 caacatttgc cgatttttgg cgtgcagttt cacccggaat cttttatcac tgaatttggt
      541 caacaaatgt tggcaaattg gcttgagatt acacaagatt tttagcgtga aatccgctat
      601 aatccgtcag ttatttaccc ttttaaaatc tgtaggggcg aaaaattttt cgccttactt
      661 aatatgcaag agataattta aatgaaagat tcgattatca ataaattaga aagtttaagt
      721 gaacgccacg aagaactaca ggcgttactt ggcgatgcct caattatttc cgatcaagac
      781 aaattccgtg cttattccaa agaatattcg caattagaag aagtggttgg cacctttaac
      841 cgctggaaaa agctgaacaa tgatattgaa gaggctcaat tattactcga tgatcctgat
      901 atgaaggata tggcagcgga agaaattgcg gaaaataaag ccgaaatcga gaatcttgag
      961 caacatttac aaattttatt attgccgaaa gatccgaatg atgaatacaa cgccttcctt
     1021 gaaatccgtg ccggaacggg cggtgacgag gctggtattt ttgccggtga tttataccgt
     1081 atgtacagcc gttattgcga aagcaaacgc tggcggattg aagaaatgtc ggcaaatgag
     1141 agcgaacaag gcggatataa agagatcatc gtgaaaattt caggcgaagg cgtttacggt
     1201 cagcttaaat ttgaatcggg cggacaccgt gtacaacgtg tgccgaaaac cgaatcacaa
     1261 gggcgtattc acacctctgc ttgcacggta gcggtaatgc cggagttgcc ggaaagcgaa
     1321 atgccggaaa tcaatccatc tgatctgcga attgacacct accgctcatc gggtgcaggc
     1381 ggtcagcacg ttaataccac cgactctgcg gtgcggatta cccatatccc gaccgggatt
     1441 gtggtcgagt gccaagacga gcgttcacaa cacaaaaata aagctaaagc gttggcggtg
     1501 ttagcctctc gtattgtaca agtcgagcag gaacgccaag ccgcggagca agcggatacc
     1561 cgccgtaact tactcggttc aggcgaccgt tcggataaaa tccgcaccta caactacccg
     1621 caagggcgtg tgaccgacca ccgtattaac ctcacggttt atcgtttaga cgaagtgatg
     1681 aacggcaaaa ttgacgagct gatccaaccg attatcactg aatatcaagc ggatcaattg
     1741 gcggcgttgt ctgatcaaaa ttaagtcaca agcggttagg ttttgccaat tttttgtaaa
     1801 attagactgc ttgtttatat cttagaggaa tcctgataat actgaaatat catgcaaact
     1861 gataacgtta ctttatctga atggcaattt ggggagaaac caaataccct agccaacctt
     1921 gtgcttgctg gcataaagac ggctacttgt tctggatatt gcttttataa gttagacaat
     1981 gagcctcttc cgcaaaaagg tgagcgacac atagtgttag acagccaaaa ttgtcctgtt
     2041 gtgatgattg aattaacttg tatagagcga aaaaagtggt tcgaaattga tgaaaagttt
     2101 gcacttttag agggggcgga ttccttcagt gagtgggaaa ggacgcaccg aaattttttt
     2161 aacgcacagc ttgaacgggc gattgatgag cagtttgaac tcgtttgtga acagtttcat
     2221 acggtaaaca ttttttcaaa aacgtatgcc caatggtttc ggtttgctga gcagactctc
     2281 cttgaaaatg ccgagcaaga tccgttttta aacgccaaat ttgatgctaa tttgttgctg
     2341 caagcagtca caaaacgctc aaaatcggcc atttttgctt tttccgaaac agtgctttct
     2401 gaacgtgagc tggtggaatt agcggaaaaa ttagctcgac gtgccaaagg tgagccgatg
     2461 gcatatattt taggctatcg ggaattttgg tcgttgccgt taaaagtttc accggcaaca
     2521 ctcattcctc gccctgatac ggaacgattg gttgagttgg cgttggaata tgctaacaag
     2581 cggttagaaa atcagaaaat gttgcaaatt ctggatctcg gtactggcac aggggcgatt
     2641 gcgttggcac tggcgagtga attaggtgag aaagcgcaaa ttatcggcgt ggatttccaa
     2701 aatgaggcgg tgcaactggc agaagaaaat cgccaacact tgggcttaaa tcaggttaca
     2761 tttttacaaa gcgattggtt cagttcactg gcaaatcaac aatttgattt aattgtctca
     2821 aatccgccct acattgatga agcagacgaa aatttgacgg tgggagacgt acgttttgaa
     2881 cctttaaccg ccttagttgc cgataataag ggattgagcg atttgcaaaa aattattcaa
     2941 aatgcaccgc tttacttaaa gccacaaggg gcgttaatgc tcgaacacag ctggcaacag
     3001 gggaatgcgg ttcgtgattt atttgattta aacttgtggg aacaggtgga aactgctcaa
     3061 gattatgccg gcaatgaccg agtaaccaaa gcgattttaa aataaggcag aaaatggacg
     3121 atattattga agaattgtta aaagagttga aagatccgaa agtaaccatc agcgaaaccg
     3181 agctgaaaaa gtttctttat cgtgaaattt tacgattaac tacgctcatt gataggaatg
     3241 taagtgagca acaagtgttt ggtcagatgt ctgcgttggt gaaaaaagct cgctataacg
     3301 tgaatggcga aggcgatgaa gagagaatta accaattact tgatttagtc tatcgtgaat
     3361 ggggctttca ttgctactat gaagactatt tccacaccga aaatttattg ctaaatcaag
     3421 tgattcgcaa acggcgtgga atgccggttt cacttggggc ggtggtgctt tatttagctg
     3481 cggtattaga tttaccgatt tacccggtta attttccgac tcaacttgtg ctacgggctg
     3541 aaatgcgcca gccgaacgga gccactaaag tgcgttttat caacccctgg aacggtgaat
     3601 ttctcactat cgaaatgctg aataaatggc tggagggtga aatgggcttt ggggtcgaag
     3661 tcacccctga tttattaaga cgggcagaac cacaagagtt gctggagagg gtcgaaactg
     3721 tgtttaaaat ggcacttacc cgtgaaggca aatatgaaga aaccttacgc ttgattgaat
     3781 accgcttggc atttagcccg gaagatccgt acgaaatcag agaccgagga atggtactgg
     3841 caagtatgga ttgctaccaa gcagcattag aagatattaa ttatttcatt gaccaatgcc
     3901 ctgaagatcc ttctgccgaa attttaaaaa tggaagtgaa aggactacaa caaaaaagcc
     3961 aagaaagttt ggtgcattaa gctgagaagc tgccccaaag gggctgagct atgattaacc
     4021 tagtacggct ttgccgtgct aggcagtaaa ataagaaaca aggactccct atgacagata
     4081 aaattgtaaa agttggcaat attgaagtgg cgaatcacaa gccgtttacc ctttttggtg
     4141 gcatgaacgt gctggaaagc cgagatatgg cactgagagt gtgtgagcaa tatgttgaag
     4201 tcacgcaaaa actaaacgtt ccttatgtat ttaaagcctc ctttgacaaa gcaaaccgtt
     4261 cttcaatcca ctcttaccgt gggccgggaa tggaagaagg cttaaagatt ttccaagaat
     4321 taaaacaaac ctttggcgtg aacatcatta ccgatgtaca cgaaatttac caatgtaaac
     4381 ctgtggcaga agtggttgat gtgattcaac ttcctgcttt tttggcccga caaaccgatt
     4441 tagtggaagc aatggcccgt accggtgcgg ttattaatgt caaaaaacca caatttttaa
     4501 gccctgggca aatggggaac atcgtagaaa agattgctga atgtggcaat gataaagtga
     4561 ttttgtgcga ccgtggcaca aactttggtt acgataatct agttgtggat atgcttggtt
     4621 tcggcattat gaaaaaagtg tcgaaaggtt gcccggtgat ttttgatgtg actcactcat
     4681 tacaatgccg tgatccattc ggtgcggctt ccggtggtcg cagagatcaa gtaaccgaac
     4741 tggcacgctc cggtatggcg attggtgtag ccggcttgtt cttagaagct cacccggatc
     4801 caaactcggc gaaatgtgat ggtccatctg cattaccgct gtcaaaatta gaagcctttg
     4861 tttcacagat gaaagcgatt gatgagttgg ttaagagctt tgaagaaatt gatacttcca
     4921 aataaacttt ctccgcatta aatcctttcc tactttcttt gcttcgccaa agaaagtagg
     4981 caaagaaagg cgaccctact tcgctacttt tcttcgctta gtgtgaatgg gtttaacgaa
     5041 aaatttacat tcgcttcgct cagagtaaat tttcctaccc attcaaacac gctcaggtag
     5101 ctcagaaggg atgtaagaaa tgtaaaaaga cagaatagga gaaaataatg ttaaatattg
     5161 tttttcttga ccgtaccggt attcctgcta ctcacgagat tcctcgcccg aattttccgc
     5221 atacttggac agaatatgct accacctctg ccgaccaaac ttttgagcgg gtaaaagagg
     5281 cggatattat catcaccagc aaagtgctgc tccaacgtga tttactggct aaattaccca
     5341 agctgaaatt aattgcgatc acagcaaccg gcacgaacaa tgtggactta gcagcagcaa
     5401 aagagttagg gattgcagtt aaaaatgtga ccggctattc caccgtcacc gtgccggaac
     5461 acgtcattgg tatgatttat tccctgaaac accgcttagc cgattaccat cgtgatttaa
     5521 ttacatcaga tcgctgggca acctgtgggc aattttgcta tgtagattac ccgattcacg
     5581 atgttcgtgg ctcaacctta ggcattttcg gtaaagggaa catcggggct gaagtggcac
     5641 ggctggcaca agcggtcgga atgcaggtta tttttgcaga acatcaaggc gcaactgaaa
     5701 ttcgagaagg ctacaccgcc tttgaagagg tattcaaaca agcagatatt atttcactac
     5761 actgcccttt aaccgagcag actcaaaacc tgattaatgc agaaacctta gccttaatga
     5821 agccgaccgc ttatattatc aatacgggtc gaggcccact gattgatgaa caggcattgc
     5881 ttgaggcatt agagcaaggc aagattgccg gcgctgcgtt agatgtttta gtgaaagagc
     5941 cgcctgaaaa agacaaccct ttaatgttag cagcaaaacg tttaccaaat ttactgatta
     6001 caccacacgt tgcgtgggca agcgactctg cggtcacaac gctagtgaac aaagtaacac
     6061 aaaatatgga agagtttgta gaaacaggta aataatttgt agataccata agatattgtt
     6121 tagatttcaa tttaccgttt cgaacaaccc tctcccctta tgggagaggg acgatttttc
     6181 ttcattcaga agaaaaatca gggagaggga acaagcgggt atttttcatc agaattttgc
     6241 aaataccccc tctctctgaa aaatatttct gaacgaaata tttttcggtc tctctcccac
     6301 aaggggagat agtggtaaac tccataggga cggaccgata tatccacccg tagtatctat
     6361 tatttttaaa agaaacagat gaatttaacc ccttttttta ttgcttttcg ctattttaga
     6421 tcgaaaagtg ctgaccgttt cgggcggtta gtcaccaatc ttgccagcct tggcattgtg
     6481 ctgggcgtaa tggcattggt gattgtgctg tcggttatga acggtttgga aaatatgcag
     6541 aaaaccaatc tgctatccac cttgccgcac gctatcatca cccctcaaga ggggcatttt
     6601 gccaaaggtg aaaaactggc tctacctgaa tttgccaagc aaagtgtggt gattaaccgt
     6661 gcgaatgtga ttatccaaag ttcacaaggc attaacgccg gtcagttaat cggcgtggaa
     6721 aatgccacag acgatccgtt gttagtggcg gaaaatatcc ccgcattatt gcctagcggt
     6781 gaatttaagg tagtaattgg ctctcgttta gccaataaat tgaacgttac ggtaggcgat
     6841 aagctccgct taatgatcac tgaaaacagc caatatacgc cacttgggcg agtgccggta
     6901 cagcgtttgt ttgaaatttc gggcgtttat caatctaatc gtgaagcgag cgaatttacc
     6961 ctctttgcta atttagctga tgtcgggcgt ttgcttcgca tcagcgaaga tcaagtacaa
     7021 ggcgtacgtt tatatttgca agatcctttc caagttaccg aacttagcca atatttcgat
     7081 gcagaacgct accaaatcag tgattggcgt gagcaaaaag gcgaattttt ccaagcggtg
     7141 aaaatggaaa aaaatatgat gggcttactc atcagcctga ttattgtggt tgcaatttcc
     7201 aacattgtga cttcactcag tttgatggtg gtagacaaac agggcgaaat tgcaatttta
     7261 caaacccaag gcttaaccaa aaaacaagtg atgcagattt ttgtgttcca aggggcgatt
     7321 gtcggagtaa ttggctcgat tattggtggt ttgctcggcg tattgatcgc ctacaattta
     7381 gatcagatta tccagcaatt aaacccgagc atacatctgc ctaccttaat ttcaggctcg
     7441 caaattgcgg ttattgttgg ggtgtcgatg cttctttcac tcctttgcac actttacccg
     7501 gcataccgag cctcaaaaat tgagccggca caggcattaa gatatgaata ataaataata
     7561 agaacggaaa taaaatgaca gaattacttc gctgtgaaca tatcagcaaa ttttacgatg
     7621 agggcgaaca gaaagttcag gtgctaaaag aggtgtcatt ttcgatgaac gaaggtgagc
     7681 tggtggcgat tgtcggtagt tcaggttccg gcaaaagcac cctattgcac acattaggcg
     7741 ggctagatca gccaagcacc ggtgaagtct ggattcgagg gcaatctctg caacagctca
     7801 gttcagacaa attagcgtta ttgcgtaacc aaaacttagg ctttgtgtac caatttcatc
     7861 acttaatggc agactttacc gcccttgaaa atgtgatgat gccgatgctg atcggcaaac
     7921 agaacaaaac tgaagctgtc aatcgtgccg agaaaatgct acaagcggtc ggattagctc
     7981 atcgaatgac acatcgccca tcagcacttt caggcggcga acgccaacgg gtggcgattg
     8041 cccgagcgtt ggttaataat ccggctctgg tacttgccga tgaaccgacc ggtaatttag
     8101 accaaaaaac cactgaaagc atttttgagc tgattcaaca gctcaaccaa gagcagaaca
     8161 ttgcgttttt attagtaacg catgatttaa atctagcgaa taaactttca cgtagtcttg
     8221 taatgcgaga tggcgtgtta agcgagggga gcttatgagt acgccgtttt tcattagctg
     8281 gcgttaccaa cggggcaaac agaaaaacag actggtttca ctaattgccc tcttctccaa
     8341 catcgggatt gcccttgggg ttgcggtatt aattatcggg ctaagtgcaa tgaacggctt
     8401 tgagcgagag ctgaataatc gtgtactttc ggttgtgccg cacgcagaat tagtttcgtt
     8461 tcaaggcaat cagcctcagc cgatttttga tagcaaaaaa ttagaggcat tggtaaaaaa
     8521 atcgcaaaat gtgaccgcta gttccccttt cgtgagcttt accgccttaa ttgaaaatgg
     8581 ggctcagctt aaaatcgccc aggtgcgagg ggttgatcct aaaaagcaag atcaggccag
     8641 ccaattaagc caatttattc cacccgagca atggcaagcg ttcaatcagc aatttcaagc
     8701 cgatgaagat ggcttgattt taggagccgg tattgccaaa gcgttagaag ttgaggcagg
     8761 agacgaagtt acgcttttaa ttcctcagcc aacagaagac ggcaaattag cccagccctt
     8821 gcgttttaac ctaaacgtaa ccggcatttt gcggctagat gggcagctgg atcacagtta
     8881 tgccttaatt ccactgaata aagctcaaga gctgatggaa taccaagcga atgaagtgtc
     8941 aggacttgaa atcagccttg ccaatccgtt tgatgtacaa cagcttgaaa tgccacaact
     9001 gaacggctat acccaaccgc tttatttgaa tacgtggatc gataaattcg gttatatgta
     9061 taacgatatt cagctgattc gcaccgtgat gtacattgca atggttctgg taattggcgt
     9121 tgcctgtttt aatattattt ccaccttgat tatggcggtg aaagacaaac agggcgatat
     9181 tgcgatttta cgaacacttg gtgcaaataa cggttttatc cgccgcattt tcttatggta
     9241 tggcttaatt tcaggtatga aaggggcgtt attcgggctg attttaggtg tgattttatc
     9301 gctaaattta accgctatta tcaaagcgat tgaagcattc tttgaggtga aattgctttc
     9361 agacggagtt tattttgttg atttcttacc aagtgaatta cattggcaag atgtcgtcta
     9421 tgtattggcg gccacgatta tcctaagcct ttttgccagc ttataccctg ccaaccgagc
     9481 cgctaaatta gaaccggcaa aagtgttaag tgggcattaa tcattttcct attctaataa
     9541 aaaagcagct acccctcgat tatgtcagaa aggtagctgc ttttatttat gttattactc
     9601 ttcgcctaat aatttcaact cacgagcctt aatttggttt tttagaattt caactaagtc
     9661 tgaaacgagg taagtaccta agtctgcacc tttacgggta cgcacagaca ctttaccttc
     9721 ggcgatttct ttatcaccgc aaaccaacat ataaggcaca cggcgtaagg tgtgctcacg
     9781 cactttgaag ccgacttttt cgttgcgtaa gtctgctttc acacgaatgc ctgcatcaga
     9841 aagctgtttc accacttgtt gcacataatc tgcttggctg tcggtaatgt tcattaccac
     9901 tgcttgggtt ggggcaagcc aagttgggaa gaaaccggca tattcttcgg tgataatccc
     9961 gatgaaacgt tcgatcgagc ctaaaatcgc acggtgaatc attaccggtg tgcggcgacc
    10021 attatcttct gctacataag aggcttctaa acgacccggt agagcgaagt caagttgcac
    10081 cgtgccacat tgccattcac gtcctaagca atcacgcaat gcaaactcaa ttttcggccc
    10141 gtagaatgca ccttcgcctt cttgaatttc atattcaagt ccgttgttgc gtaaggcgtt
    10201 ggctaagcct tcttctgctc tgtcccacat tgcatcatca ccgatacggt tttccggacg
    10261 ggttgaaagt tttactgcaa tgttagtaaa accgaaggtg ctgtaaatgt cgtacaccat
    10321 tttgatacag ctggttactt ctgactcgat ttgttcttca gtacagaaaa tatgtgcatc
    10381 atcttgggta aaaccacgca cacgcattaa gccgtgtaat gaaccggacg gctcgttacg
    10441 gtgacacgaa ccaaattccg ccatacggat cggtaaatca cggtaagatt tcaagccttg
    10501 attaaagatt tgaacgtgac ccgggcagtt catcggctta attgcatatt cacggttttc
    10561 cgattgggta gtgaacatta aatctgcgta gttttgccag tgtccggttt tttcccaaag
    10621 cacacgatcc atcataaacg gaccttttac ttcttggtaa tcgtactctt tcaatttggt
    10681 acgcacaaaa gtttcaagtt cacggaaaat cgtccagccg tcattgtgcc agaacaccat
    10741 acccggtgct tcttcttgca tatggtataa atctaacgct ttaccgatac ggcggtggtc
    10801 acgttttgcc gcttcttcca aacgttgtaa atattcggct aattgttttt tatcagccca
    10861 agctgtaccg taaatacgtt gtagcatttt atttttgctg tcgccacgcc agtaagcacc
    10921 cgctactttt tgtaatttga agtggtggca gaaacgcata tttggtacgt gtggaccacg
    10981 gcacatatca atatattctt cgtggtgata taacgcaggc gttgccgtac gttcaatatt
    11041 ttcatctaaa atcgccattt tgtatggctc accacgtttc tcaaaggtat ctcgagcttc
    11101 ttgccagctt accgggcgtt ttaccacgtc atagttggtt tttgccagct ctaacatacg
    11161 cttttcaaga gcgtctaaat cttcttgagt caaagagcgg tctaaatcga tatcgtagta
    11221 gaaaccgttt tcgatagtcg ggccgatagc cattttaacg tctgggaata attgcttgat
    11281 ggcgtgacct aacaagtgag cggttgagtg gcgaatgatc tctaaaccat cttcgtcttt
    11341 ggcagtaatg atttcaagtg cgctgtcttg gctgatgata tcagacgcat cacggcgttc
    11401 accgtttaca cgaccggcaa tggtggcttt cgctaaacct gccccgatag attgggcaac
    11461 ttccattaca gaaactgggt tgtcaaatga acgttgtgaa ccgtccggta aagtaataat
    11521 tggcataata aatccttaca gtggtaatcc atacgagaga ttacttgtgt tgtttgaatg
    11581 aaaaaaccgc ctacaagcgg tcttttttga ttaaaatttt gcaatttgct tattatttcc
    11641 cttacaagtg gggaataaaa gcactgaaat tctagcataa aacaaagaaa aattaaactt
    11701 tgctgttttc agcggcctct tcatcaagct caatattttc attttctgtt tgtggatctt
    11761 gttcaagtat ttcaagctga gcctggtact cttgtaccgc ttcttcatca aatggaataa
    11821 gtgcgtcctc aacaatttca atttcttcaa gtgggcttgc ggttgcaatt ggggtagcat
    11881 attgcatctc tgctcgttcc gctttcggaa ttgatttggt gcggaaagca caaataaata
    11941 aactaagtga gaacagtcca aaaagtgcat aaaagccata ttcagcggaa aggctcatcg
    12001 caaaaccgat aagcggtgga gcaactaacg cccccacacc atagcaaaac agtaaacccc
    12061 tgcttacttc aaccgtgttc atattattcg gcaattcatc gttggcacgg gcaatgctca
    12121 aagcatataa gctaaacaga ctacagccaa agaaaaaggc gataatatac tgtccccaag
    12181 cggtcaaatt ttcttggaaa attgcaaata atccggcaag tgcggttaaa gtggcaatca
    12241 ctgaacaagc taaaatggcg ttacgtctgc caatacgatt cgataatatc gccatcggca
    12301 tttggctgat aaaaccaccg atcatcgaga tcatcaaata aagcgagatt tcacttaatg
    12361 aaaagctaaa cttcagcaga aagactgacg ccattgtaaa aaagccgttt accaacattc
    12421 cgctaataaa gctggttgct aaggctaacg gtgaaatcgc aaatagttta ggaatactaa
    12481 tgcgctctcg agccggaata tcaggctgaa ccattttagt taaagcaatt gggatcatcg
    12541 acatcacaac caaaatggta gataagataa aaatgtttcg gctactaagt tctaaactta
    12601 ataaacttac gcctaaaata aaggcgagat agaagataat gttataagtg gcaagcactt
    12661 tgcctctggt atcgacattg gttttttcgc ttaaccaact ttcgactaaa agcagcaagc
    12721 tataatgaca gaatcccaaa ataattcgta atacgcccca gatccacaaa gtatcaatca
    12781 taatatgacc gagagcggcg atagcaaaaa cagcacagaa aacgctaaat gaacggatat
    12841 gcccaacact tgatacaatg cggtgagcaa aaatagtgct taatgccgcc ccaataaaga
    12901 aagcagcgtt aagtaaaccg atgatgatgt ccgttatccc catttctgcc agtttgatcc
    12961 ctgctgaatt tagaaataaa ccgtagccga aaaacagaaa aaagatacct gaataaaggg
    13021 agtaaaattt agtgctattc atcaatgctc cttcaaacat tctgccaata ttaaatcatt
    13081 gtaccgattt aatacctttt aggcaaaaaa gttattatta gcgttgagaa aataactttt
    13141 ggctattttg attaaaaaat caaatgaagg ggattttgaa atgtagtaaa aaatagagca
    13201 atcagaattg aaaaaggctt tgccgtaaca aagcctttag aaagattaga gtttcgagaa
    13261 gaatttgtag cttgggctaa ccaaaacact tgctaggagt gctttaagca caccacctaa
    13321 aataaatgga tataagcccg cttgtaatac tgtgccttct ggtacaaaga aagaaagttg
    13381 agccaagccg aaagcaaagg tgatcacact tgcaattaag gcgacaaata aacttttcca
    13441 aaaagagttt aatacgcctt tatcgctata atagcctaag aaataggcgg ttggaatata
    13501 gcctaataag tatccgcctg ttggaccaaa gaaatgatgg aaacctgaag caccgccggc
    13561 aaagacaggt aaaccaatcg ccccttcaaa aagataaagt aatactgcag cagttgcggc
    13621 tttacgaccc attgcaaaac caaccacagt taaagcaagt gtttgccctg taatgggtac
    13681 agggtacatc ggcacagaaa tttgtgcaaa aatagcaagc agtgttgctc caataaaagt
    13741 tttcaaccaa aatgtagtta tactattggc ttctagccaa cgtttagttt gaattaattg
    13801 cgtagtgaac ataatccttt cctttaatat ggttaaaatg ttctcaaccg attatatagg
    13861 aagatgtctt ttagggtaag agtgaattta aaatggtaaa accagtttat atttattgtg
    13921 atgaaggtag cagtgaaatt ggtgttcaat cactgttatt ggcagtaaaa cagcatttac
    13981 agcgtccggc ttatctcatc aatgccaaac aaattattca aagcaagttt tccgatggct
    14041 gtttattaat catgcccggt ggagccgatt taccttactg caaaaaatta aacggagaag
    14101 gcaatcgtag aatccgccaa tttattgaac aaggtggagc ttatctcggt atctgtgccg
    14161 gagcttatta tggctgtgct gaattgaatt ttgtagggcg tgactatagc gtaaatggta
    14221 atcgagaact agctttattt aatggcattg cgaaaggcag tctgccgcag tttaccaacg
    14281 ggcaacttta cgataaaaac attggtagca aagcgatagt tgaagtaaca cttgcagagg
    14341 gtggaaaaat gccgttttat tatcacggtg gttgctgttt tgaaccaaat tcaacaacag
    14401 tgatttacaa cccgttggct tattatcctg atggctcagt agcaatgata gagggagagc
    14461 tagggcgagg ttattatctg ttgtcgggtg tacattttga attacaagcg gtcgtttatt
    14521 ggcaaaaaat tgtaaattgc tgtgaaaatc ttgacataaa aacacaagaa gaagcaattt
    14581 gctctaattt taatgtaaat tatggctcgc aaatttggca acatctaaag caacgactaa
    14641 atcaagcgta aaaaaataac ctttcgctat tctgccaaat ggtttctgca atcagatttg
    14701 gtgattctgt tcttaattca catagactat tgaaagtcac taaaagccgt tccggtcggt
    14761 tcggctggcc ttgaaagcca aacaccggca tatcgggcga gtcggtttcc agtagtaaac
    14821 tttcaagagg tagtttgcgg atagcctcac gggttttgtt agcacgttca taggtaatag
    14881 ttccccccac accaatttta taaccgagat cgacaaaacg ttttgcctgt tcatagctgc
    14941 cggcaaagcc gtgaaccaca ccggttttgg ttagttgggc ttttttgagg aacacaaaaa
    15001 gttgatcgtg gcttttacgt gaatgaagat taacgggtaa attaaatttt ttcgcgaggg
    15061 aaagttgtgc ctctaaaaat tcacattgct ttttccataa ttcaggggta gaaacttcac
    15121 ttaccgcttt ttccaaaccg atttcggcaa tagcggtaca ctgtggatct tgttggctta
    15181 gcaaattttc caataagatt aaatcttgtt gttggtgctg ttgaatatac agtggatgca
    15241 accctaatcc atacaccaaa ttttccggtt tttgtttggc acaagcggtc acttttgcaa
    15301 aattttttgc aaatacggca acgataagaa tgcattcaac ctgatgctgt tcggcattct
    15361 caaccaattc cggaatgctt aaattcaggc tttctgcgag atagtcgagg tgagtatgag
    15421 tgtcaaaaaa tcgcataata aaaccaataa aaacggggac tagattgtcc ccatttttag
    15481 cattttacgc aagcaattag tttgttacat aagcatcttc atcacgagcc tcagatggct
    15541 tgaggaaagc aaagaagcaa ataacacaaa ctaccgtaat cgcaaaaccg atataaactg
    15601 ataattcata atctaaaccg aagccgattt tgttgttcgc aaggtaagtt gcacacacgg
    15661 tagtgataaa ccatgcagga atcgagcata cccagtggaa ttttttatag cggtataagt
    15721 aacctgcggc agtccataac ataaccattg cagtcatttg gtttgcccaa gtgaagtaac
    15781 gccataatac gctgaaatca acttttgaga caatgaaacc aagtacgaat aatggtacgg
    15841 caatatacaa acgttttact aaggtacgtt gttctaaacc aaagatttcc gctagttgta
    15901 aacgagcggc tcggaatgca gtgtcacctg aggtgattgg taatactaca acgcctaata
    15961 cggcaaaaat accaccgata aaccctaaga agtgtaacga gctgtcatat accactttag
    16021 acggtgaacc ggcagagatc gcatcttgta aggcttgtgg gttttcatag aatgaaagac
    16081 ctactgcaca ccatactaat gcaattaccc cttcagtaat cattgcaccg tagaaaataa
    16141 aacgaccttc tttctcattt tgcgcacagc gagccattaa tggggtttgg gttgcgtgga
    16201 aaccggataa tgctccgcaa gaaatagtta agaatagtaa tggccaaatt ggaacatcac
    16261 ctttcacttg gaagttttgg gtgaattttt cccacgttaa gccttgaccg tctgcattca
    16321 ctgtacggaa gaagtcgata ggatcagtcg cacttaagtg agcgctgact agaccatata
    16381 ccatacctac agacataaag agtaataatg ctccgaagaa aggataaatt cgaccgataa
    16441 ttttatcaac aggtagtaat gtagcaagaa tatagtaagc aaaaataatt aacgtccaca
    16501 cagaaatcac ggttgcttta tccatacccc aaacggttac gccacctgat gctacagatt
    16561 ctttaattgc ttcaacatca cctaattcca ttgcactttg ggttgcacct gcaatatcca
    16621 tggtgatggt agagagtaac tgagcagggc ttgcaacgaa aactacgcct actaataaaa
    16681 gcaagatgag cgccattacg ttgatgaata acttgactgg acgacccaag aatttccctg
    16741 ccagatttgg catcgttgcg ccaccgttgc ggatacttaa cataccacag aagtagtcgt
    16801 gtactgcacc ggcaaaaata caaccaatca caatccaaag cattgcaact ggtccgtata
    16861 aagcaccaag aatcgggccg aaaatagggc cggtgccggc aatatttaat aattgaatca
    16921 gccaaatctt ggttttagac attggcatat aatccacacc gtcattcata gagtaagccg
    16981 gtgtcgatct ttttgggtta atcacgaaga ttttttcaat gattttccca taagtgaaat
    17041 agccaagcac aagcacggct acacagaaaa agaaccacaa catagtagaa atcctataaa
    17101 ttgagataaa tgatcatttt tagcagaact gctaaaggcg tgccattgta agtgaggagt
    17161 gaggataaag taaaacgaat tgttagattt gtgtgaataa tttactgaaa atgtgaggtt
    17221 catcaaaaaa tccctttgtt ctgtatcaat caaagggact ttttaaagtt aaatatcgaa
    17281 tttttgaatt tttgcttcaa tgccttgggc atctaatcca agatcggcat aagcttcagc
    17341 ttgagtagat tggggaacga aaaagtcagg aataccaagc attacaagcg gtttgatttt
    17401 gccgatattt tgcaaatatt cattcacaaa actacctgca ccaccttgaa tagcattttc
    17461 ttcaagcgta accaacaact cgtgagaatc cgccactttt tgaagtaatg cttcatctaa
    17521 tggtttaatg aaacgcatat ctaccaaggt gtagttatgt ttttcagcca caattttggc
    17581 ttcatcaagc aaagcaccaa aattcaaaat cgcaactttt ttaccttcac gaataatgtt
    17641 tgctttacct acttcaagtc cttgcattgg ctcaagtttt actcctttag cattgcctcg
    17701 agggtaacga atggcacttg gcgtattcat cgtgtaagcg gtataaagca tttgtcgcat
    17761 ttcattttca tcgctcggtg ccataatggt catattggga atacaacgca taaaacttaa
    17821 atcaaatgca ccttggtggg tttgtccgtc tgcaccgaca attccggctc ggtcaatcgc
    17881 aaaaatcacc ggcaggtttt gaatagcaac atcgtgaatt aattggtcat aagctcgctg
    17941 caagaagctg gaataaattg cgactaccgg tttgtaacct gcgattgcta agcctgcccc
    18001 gaaagtcact gcgtgttgtt cggcaatcgc cacatcaaaa tattgttcag ggaaacgttt
    18061 ggaaaattct accattcccg agccttcacg cattgccggc gtaatgccga taattttcgg
    18121 atcacgctct gccatttcac atagccaatc gccaaaaata ttggaataag taggtgctgc
    18181 ttttgatttt ggtaactcgg ctgcggtagg gtcaaatttc ggaacgccat ggtagccaat
    18241 cggatcattt tccgcaggtg tgtagccttt gccttttttc gtgcgaatat gcagaaattg
    18301 agggcctttc agctcacgca tattgctcaa ggttttgact aactcgtcaa tattatgtcc
    18361 atcaatcggg ccgatataat taaagcctaa ctcttcaaac aaagtgcttt caggggagaa
    18421 aatcacccct ttcatatgct cttcgctttt tttcataaag tttttgatgg taggcacttt
    18481 atctaaaatt ttcttactgc cgtcacgcac tgaagtatag agcgaaccag aaaaaatgcg
    18541 ggcaaggtgg ttgtttaatg ccccgacatt ttcagagatt gacatttcat tatcatttaa
    18601 gataaccaac atatcggtat gcaatgcacc tgcgtggttt aatgcttcaa atgccattcc
    18661 ggcagtgatt gcaccatcac cgattacaca gacggtttta cggccggcat tttctttttc
    18721 tgctgccacc gcaatgccaa gtccggcact gatcgaagta gaagaatgcc caacgcttaa
    18781 cacatcgtaa gggctttctt cacgccaagg gaaggggtga ataccatctt tttggcggat
    18841 agtgtgcatt tgatctcggc gcccagtaag gattttatgt gggtaagctt gatgaccaac
    18901 gtcccaaatt aattggtcga aaggcgtttg ataaacataa tgcaaagcta ccgtaagctc
    18961 tactacccct aagccagagg ctaaatgtcc gctagtttga ctgactgatt ccaataaata
    19021 ggctcgcagt tcatcaacta cggtttgaag ctggtcttta gaaagtaaac gtaaatcttc
    19081 cggtgagttg atttcgctta acagaggata cttcttttcc ataatgtttt attacccaca
    19141 accatcaaat aatagatgat attaaattaa gctttgagtt cttcggtcaa agtaggctga
    19201 atgctattta aaatttcttt aaccacacgg ccgttaccaa caaccagatt acctgttttc
    19261 aagtaatctg ttccgccaac aaagttagtc acaattgcac cggcttcacg ggcaataagt
    19321 tcaccggcgg cgaaatccca cggttttaag ccgatttcaa agaaaccatc aacacgaccc
    19381 gctgcaacat aggcaagatc taaagcggca gaaccggtac ggcggaaatc agctactccg
    19441 ttattcatta agccttcaag catatttaaa tgtgcatcac gatgtctgat gactttgaaa
    19501 ggaaaaccgg ttgcaattac agtgcctttt aaatcacggc gttctgcatc aatgcgtaaa
    19561 cggaactcgt ttaatttagc cccttcgcca cgcaccgcag tgaaaagctc gttacggata
    19621 ggatcgtaaa ctacccctac tgttgtacgt ccgttttcac gcacggcaat cgacacagca
    19681 aaatgaggta agcgttttac gaaattggtt gtaccatcta atggatcgat cacccattgt
    19741 acattttcat tgctaccgtt taacacgccg gactcttcgg tcacgatcga gtgttcaggg
    19801 taagatttac ggatcacatc aataattgcc gcttccgctg ctttatcgat ttcagtcaca
    19861 taatcattcg tatttttttg taaaacttgt gtatcttgag gcgattgttc atagcctttg
    19921 gcaataatat tgcctgcttt tcgtgccgca cgaatagcaa tgtttaacat tgggttcata
    19981 tttccaccag attttaaaga acataataaa gcacgaatta taaagaatgt agcgaaataa
    20041 agaaacaaca agcggtctaa tctgtgaaaa attttgcaaa aaaatggctt tttctaaccg
    20101 cttggagttt ggattcgtta taatacctct cattcaagcc acccatttag gtactaaaaa
    20161 tgcaatttaa agaaaataac caacctcgtt ttaaacacag agaagaacgc cgttcagatg
    20221 agcgttcccc ccgtttttca gataaaggca aaatgcgagg aaatgaaggt aaaagtgaca
    20281 gaccgcataa gctacatttt aagcgtgacg aggcttattc acgctctgag cagatttatc
    20341 cagcagaagg caaaaaagcc agtggtgaag gtagtgtgca aatttgggta aaaggtggga
    20401 atagctcagt taaagagaaa aaaaacggcc cgctttcacc gagagcgcct gagaaaatta
    20461 agaaaaatcg cttggaagag atgaaagtct atggcgaaaa tgcctgtatc agtttgttcc
    20521 aacaacgtcc tggatctatt gtacgtttat gggcaacagt ggagggggca aaaaaattag
    20581 gcgatttaat gagctatttg gctcagcaca aaaaagccta tcacgttatt gaccgtgaag
    20641 agatggagcg agtcaccggt actgaacatc acggcgatat gtgcttattg gtgaaaaaaa
    20701 cggcttcatt tgtgctagag ggctatttac aagtgccgaa aaaacaagat tgcttggtac
    20761 tgttagatgg tgtaaacaat gctcagaatg tggggggaat catcagaacg tgtgcttttt
    20821 atggtgtgaa aggcattatt actgaaaatg tggatagctt aaactccagt tctgcagctc
    20881 gtgtcgcaga gggtggtttg gaatttgtgc gagcgttaga aactaaaaac aagcatattg
    20941 ctttggtaca actccgccaa gccggttatc aaattgtcca cctcacacgc cataagcaag
    21001 ccccttcttt aaccaaaatt aagctggcgg aaaaggtggt ttttgtatta agtgagattc
    21061 ctgcaagtga tgtggcttac gcagaagaca caacaataca gctttccttt aataatccgt
    21121 taaatagtgg tttaaatgtg gcggtaaacg caggtattct acttaatcaa tggtatcaaa
    21181 cccacattct ttaaggaatt gaaatgaatc agcaaaaacc gtttgaagtg attgcgattg
    21241 ttggcacacc tcgccacgac agtgcatttg aaacacatct tgccgtttat aattggttaa
    21301 aagatcgtga ttatcaaatt ttagtggaaa cagaggttgc cactcagtta cagcttaagg
    21361 aaagctacag tttagatcag attggtcagc gcgcagagtt ggtgattgtg ataggtggcg
    21421 atggtaatat gcttggcatc gcccgagctt tggctaaata ccgagtgcca ctgataggca
    21481 ttaaccgtgg caatctgggt tttttaaccg atatcgcacc tcaaacggct tttgaacagc
    21541 tttatagttg cttagtcaaa aatgagtatg tgattgaaga gcgattcctg ttagaggctt
    21601 tcattgagcg caacggtaaa attattgccg caaataatgc cttgaatgaa attgtggttc
    21661 acccaaccca agtggctcga attattgaat ttgaagttta tattgacgga aaattcgctt
    21721 tttcacaacg tgcagatggc ttgattgtgg caacgcctac aggctcaacc gcctattcac
    21781 tttctgctgg tggcccgatt ttaaccccga atatgaatgc aatggcatta gtgccgatgc
    21841 accctcacac tctttcgtct cgtcctttgg tggttgatgg cgatagtcaa atttcgcttc
    21901 gttttgccaa atataatacc cctgatttag aagtgatttg tgacgggcag gtgaatatgc
    21961 actttacgcc tgaagataga gtcttggtgc gtaaaagccc ggataaatta cagctgctcc
    22021 atttaaaaga ttataactac tttactgtat taggttctaa attaggctgg gcaagtaagt
    22081 tgttttaaac acaagcggtg ttttttctga ttaaatttgc aaatgcaata tgaattgatt
    22141 tcatatacat ttcaaaaata tatcattttt attcaataaa aatttgtggt tcaataagct
    22201 atctgaaaaa taaaggggac aataaaatga caacaaattt taaatgccaa gtgcatacta
    22261 ttgaatttaa tgaaaattac caaccggagc aaagcacccg tttaaccacc aactttgcca
    22321 accttgcccg tggcgaaaac cgcaaacaga acctgcaaaa tgcgattgcg atgatcaata
    22381 accgttttaa cgatttagcg aactgggata acccgaatgc ggatcgttat tcggtgaaat
    22441 tggatattgt ctcactggat attgatgtgg aaggcaaagg cgagtttttc cctgcgattg
    22501 agtggctgaa aacctatgtt atcgactaca aaaccggcaa aaccattgat ggcattatcg
    22561 gtaataactt ctcttctttt gtgcgtgatt acgatttcag cgtattgttg ttagatcaca
    22621 ataaagacaa accgcaattt agcgtgccgg aaggctatgg cgagttacac ggcaagctgt
    22681 ttaagcattt attggcatcg cctgaatata ccgcaaaata taaaaaacca ccggtgatct
    22741 gtttaagcgt ggcaaccacc cgtacctaca caagaaccac taacgttcac ccaattttag
    22801 gtgtggaata tctgcaagat agttattcat taactgatgc ctattttgcg aaaatgggct
    22861 taaaagtacg ttactttatg ccgccaaaat ctgttgcacc ctatgcgttc tactttgatg
    22921 agagtgcaga tttactcaat gattacacta acttagagct aatcagcacg attgccacaa
    22981 tggaagcgtt ccaaaaaatt taccgtccgg aaatttacaa tgccaattcg gtcgcagggc
    23041 aggtctataa aggcagcctg acttaccaag attattccgt caccaaagtg gaatatgacc
    23101 gtgaagaacg cacccagttg gcgatcaaac agggtaaata caccgaagag cattttatta
    23161 aacgttacca agcgatttta gaggcttggg cgacggagga ataaacgtag ggtgggcgta
    23221 agcccaccat aaaatgagat ataaccaatg gtgggcttac gcccacccta ctttttatag
    23281 gaaaattcaa aatgagcaac attcttctcc caacctctac cgccggcagc ctaccaaaac
    23341 cttcgtggct tgccgagccg gaaaaactgt ggtcaccttg gaaatttgaa ggcgaggaac
    23401 tacttcaagc caaacgtgat gccctccttg tttcccttgc cgaacaaatt gcggcaggga
    23461 ttgatattgt tagtgatggc gaacagaccc gccagcactt tgtgaccacc tttatcgagc
    23521 atttggaagg ggtagatttt agcaagcgtg aaattgtgcg cattcgtaac cgttacgatg
    23581 ccagcgtgcc gagcgtggtg ggggcagttt cccgtccgaa atcggtgttt gtggacgatg
    23641 ccaaattctt gcgtgcgcat accgaaaagc caattaaatg ggcattaccc ggcccgatga
    23701 cgatgatcga caccctctat gacggtcatt acaaaagccg tgaaaaactg gcgtgggaat
    23761 ttgccaaaat cctcaacgaa gaagccaaag agttggaagc ggcaggtgtc gatattattc
    23821 aatttgacga accggcgttt aacgtcttct tcgatgagct gaacgactgg ggcgtggcag
    23881 cgttagagcg tgcagcggaa ggcttaaaat gcgaaacggc ggtgcatatc tgctacggct
    23941 acggtattaa agccaatacc gactggaaac agaccctcgg caacgaatgg cgtcaatacg
    24001 aagagagttt cccgaaactg caacaatcaa aaatcgatat tatctcgtta gagtgccaaa
    24061 actcccgtgt gccaatggac ttaatcgaat tagtgcgtgg caaaaaagtg atgctgggag
    24121 cgattgatgt agcaactaac attatcgaaa caccggaaca agtggcagat acgttacgca
    24181 aagcattaca atttgttgat gcggataaac tctacccaag caccaactgc ggaatggcgc
    24241 cactttcacg ccaagtcgca caaggtaaat tacaggcact cagccaaggg gcagcgatat
    24301 tgcgtgcgga attcgcaaaa taattttgcg acctgcatcg aaaatatcaa ctttgctctt
    24361 tgctcgtttc cagattttct tccaaaataa caggaaatga cggaggcact atgctaaatc
    24421 tatttaaaaa gccaattaag aaaagctcct ttgaggcttg ggcaaaacta atggacgatg
    24481 tggcaaaagt tgcaatttta gcaattcctg ttattctcta tggagaatat tcactgctaa
    24541 tgaaatttac acactcaatt accttatttg cagtagttta ttttttgtta gcaacggcaa
    24601 gattgtttcg agaaacagca gaggagatgt aaatggacgg attgacaatc ggattatttc
    24661 ttttgcttgc attttcattt gctgcatacc aatgggcaaa gcatttaaaa gatcataacc
    24721 attaattcaa tgaataatac taagcggtca tatttggtaa attttttaca aaatgtgacc
    24781 gcttgttttt ctttcactcg aagttaattt ttatataaat aatactatct atgaaactaa
    24841 aaaatgcgta aaataacgtg aaatcaactt acgcaaacgt ttacttaagc ttattaggag
    24901 tccaaaaatg ttaaaacgtg atatgaatat cgctgattac gatgcagtat tatggcaagc
    24961 cattcaagat gaagatcgcc gtcaagaaga acatattgaa ttaattgcat ctgaaaacta
    25021 tgcaagccca cgtgtaatgc aggctcaagg ctcacaatta accaataaat atgcagaagg
    25081 ttatccgggt aaacgttact atgggggttg tgagtatgtg gatattgtag aacaacttgc
    25141 gattgaccgt gcgaaagcat tgttcggggc agattatgcc aacgttcaac cgcattcagg
    25201 ctcacaagcg aatgcagccg tatatatggc gttgcttaat ccgggtgaca ctattttagg
    25261 tatgagttta gcgcacggcg ggcatttaac ccacggtgct tcagttagct tctctggtaa
    25321 aatttataaa gcagaacaat acggcattac tgatgaaggc ttaattgatt atgaagcatt
    25381 acgcaaacaa gcccaagaag tgaaaccgaa aatgattgtt gctggtttct ctgcttattc
    25441 tcaagttatg gattgggcaa aaatgcgtga gattgccgat gaagttgggg catatttatt
    25501 tgtggatatg gctcacgttg caggcttaat tgcggcggga gtttatccaa atccgcttcc
    25561 tcacgctcac gtcgtgacga caacaaccca taaaacctta gccggccctc gaggtggttt
    25621 gattttatct gcatctggcg atgaagaaat gtataagaaa ctgcaatctg ccgtattccc
    25681 tgctggtcaa ggtggaccat tgatgcacgt tattgcggca aaagcggttt gttttaaaga
    25741 agctttagag ccggaattta aggcatatca gcaacaagtt gtaaaaaatg caaaagcaat
    25801 ggttgaggta tttaagcaac gtggttttga tgtggtttca aacggtacgg aaaaccattt
    25861 attccttgtt agctttgtaa aacaaggttt aaccggcaaa gcggcagatg ctgcattagg
    25921 agctgcgaac attaccgtga ataaaaactc ggtgccgaac gaccctcaaa aaccattcgt
    25981 gacttcaggt attcgtgtgg gtacgccttc tgtgacccgc cgtggtttta aagaagcaga
    26041 ggtgacagca ttagccggct ggatgtgcga tgtgctagat agcattggta aggataatca
    26101 cgaacaggtg attgcagaga ctaaagcgaa agttttagac atttgtaaac gtttaccagt
    26161 gtacgcataa tctcaacttt acactttttc aagcggtaat atttttagaa atattatcag
    26221 taataacgag taacaaaaat tacttatctt tcaatctatt ataagtgatt tttatgtaaa
    26281 cgttccgaaa aaatgcaaaa tgttccgaaa ctgtctttta agactggtgt tttgaggagg
    26341 gaacactttc tttgataggg gttactattg gggcttttcg aatgtatgtt ttggtcattt
    26401 gaacgctagt atgacctagt tgttggcgag cggcttcttc acctagagaa agggctttat
    26461 ctgttcctga tttagctctt agatcccgaa attggaatgc cattatttca tttgcgtatt
    26521 ccgggttatt cttgaccgct tgttcccgaa tataagtgaa ccttttacct agttttctag
    26581 cggttagctt ttcccctttc ttagtgctga ataagtgtgt tccagtagct ttctccagcc
    26641 tatcttggat aatatcagcc agttttccca ctatctcaat acgtagtttg gcttgtgttt
    26701 tttgctgaac aatatggaga taaccatcaa aaatgtgttc tttccggata ttgaccaaat
    26761 cgacaggacg ttgtccggtg atataggcga gatccattaa gtctctcata tcctgatctg
    26821 ccacttcata taccatctga aatatattgt tctcaacgta aatttcacgc tttctgatag
    26881 ggtagcgttt cattccgata ttagggcttg gtaagtttgt ataaccccac tctcgagcgt
    26941 attgccaaat atgactgaac attgaaaatt cgttattagc agatcccggt gtgtcttttc
    27001 gccaagtcat atactctcgg atatgttgag gggtgatttc ttcgagagga gcgtcattaa
    27061 agaatagcag tagatttttt atcttgcata aatctgtttg tgctgtgctc gcttttttct
    27121 gaggtataac ttcatctcga taacgtttgg ctacaagccc gaaagtaatt ctggcagttt
    27181 ggctttctct ttctatattg agttgagcag ccttaataaa agcctcattt ctatttgtgc
    27241 ctagagagat ttccgttttt ccgtgcgtgc gatatagcca ataaatgaca gttttgcctg
    27301 attttcgtgt aactgttttt ggacgtaaat tctcaggtaa gtagcgattc tgttgtttgc
    27361 gtggtcttcc catttttcac tcccattaaa taagcaggtg atttccattt cccagtggat
    27421 tgagcttctg gatattgttg gcgggggtgc ccatagacat ctttgtatag cactaatggc
    27481 ttatacttgt tttcatcttg aattatatag tgacgctccc tcaaccattc gatttgtctt
    27541 ttcttttgac gatagcctgt taaatcagca agttcgtctt tcgttaaaaa ttcagtaggt
    27601 tgtt
//
LOCUS       DANJWC010000011        27486 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.011, whole genome shotgun sequence.
ACCESSION   DANJWC010000011 DANJWC010000000
VERSION     DANJWC010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 27486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27486
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.011"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..733
                     /locus_tag="PWX02_000338"
     CDS             <1..733
                     /locus_tag="PWX02_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL1728392.1"
                     /translation="ARSSYFARLAEWTLLEHLLVKQPENISVCSFGQSFGTSGRLDAE
                     YYQPKFASLFAQLANFKTMKLGEIVNLQKSVEPGSEAYQTEGIPFVRVSDLSKFGISQ
                     TDKYLHPKDFGNVVRPKKDSILLTKDGTVGIAYRVPQDLNVITSGAIVHLELKTDEVL
                     PDYLALALNSPAVQLQAERDAGGSIIQHWKPSEILDVVIPVLPKNIQQTISDKVQQSF
                     ALRVESEVLLEKAKILVEQEIENMR"
     gene            736..1370
                     /locus_tag="PWX02_000339"
                     /pseudo
     CDS             736..1370
                     /locus_tag="PWX02_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1587..2336)
                     /locus_tag="PWX02_000340"
     CDS             complement(1587..2336)
                     /locus_tag="PWX02_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1728393.1"
                     /translation="MIKIENLTLAHGIQHLSCYLPKGKLIGIMGANGAGKSSLLKAIA
                     GILRPTSGEIWLENCKLHALSAVERSRQFAYLAQQLNVAWQLSVYDVIALGLPAPLSP
                     EQERQKVRSVAEQLAISHLLDNTYQCLSGGEQTRVQLARCLMKQAPILLADEPIAALD
                     PYYQIDIMQQLKALTPTQTCVVVIHHLPLAYRFCDEIILLKNGNLLANGNTQAVLTAE
                     NIATAFGVKAEMDWEKREMWGIEKLEMNFQF"
     gene            complement(2333..3301)
                     /locus_tag="PWX02_000341"
     CDS             complement(2333..3301)
                     /locus_tag="PWX02_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL1728394.1"
                     /translation="MKKLNIVLGSLLLLIVSSAAFYQLGDFALLKNAEGVLSDLRWLV
                     LGEIRLPRIGLAVLTGASLALAGNAMQGVFQNPLASPGLLGSSSGATAVSVFLLYYFS
                     APMGWLLFGGVAGALGAFLLVWLISFRHGTTMMILSGVAVNVLLGAAVTLLLSNAESP
                     WALAELYRWLQGSLVWATAEAVCWAFPLILLGIVCLYRERRYLDLLTFGEETAATMGI
                     NLQRSFFTVSIGVALLVGATIPQTGTIGFIGLIAPHLARIWLKKPPSQLYLTSALIGA
                     LLLLIADLAVQYVPFFARIYIGTLTAILGAPFLIWILFTQQRRLAQ"
     gene            3482..3819
                     /locus_tag="PWX02_000342"
                     /pseudo
     CDS             3482..3819
                     /locus_tag="PWX02_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253677.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3800..4123
                     /locus_tag="PWX02_000343"
     CDS             3800..4123
                     /locus_tag="PWX02_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HDL1728395.1"
                     /translation="MNELYDDPIPQATREKYGITEFDAAEYLTNDELIALYLAETLKD
                     GTDEEFIQALNTVARAKGMNELAKKAGIGRESLYQSLSSSKPRFETIRKIISALNLEL
                     SVVKA"
     gene            complement(4113..5999)
                     /locus_tag="PWX02_000344"
     CDS             complement(4113..5999)
                     /locus_tag="PWX02_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDL1728396.1"
                     /translation="MTQLDSVTVYSAYATPINQDKTASSVTVLTEKDFAARNATYVSD
                     VLKTVPGVAIGQQGGRGTLTSLFLRGAESRHTAVVIDGVKVNPINIGNFDFGGLPISN
                     IERIEVLRGEQSALWGSSAMGGVVYITTKSGLYKEKPFNAEVDLGLGSNNTRDASATL
                     SGFHNGFYYALHGDSHRTKGISALSKNHFSYTTETGSEVKTGGASERDGFHRDNGSLR
                     LGYDLGNKGVEVLAAQSSQTVHIDGYNSDVSGEYSRTRNQTFKLGGYWGNEQELLKHQ
                     ANISQFNSKATHFGSNARYSNEKQLNANYQLDVNFDREGEVTQAVSLLTDYAKTRYTS
                     DKYLREKTLSEKSAALEYRLFTEQDHSFSISGRYTDNSQFKNSITGRISGAYRLSPNL
                     CSDRLLLELAEPQQIRAMSPYSQKPLMMLDKLNTDKPTVEPELTALLPYADSTILLNE
                     TFYPQLTARLKQLGFKLVALNDSPQTPEQLFTLILQLGELTQNQAKAEKLVERLRLQK
                     IPLKQPLAETLILSETGMIEPHFPQYQTLLHLLGLSPLKSDLTPQNFSLEKLLLAQPK
                     QLLFLTDNQSYNNQAELLKHPALQKIWQKMSQNPPLVLPMKYTYCFDHGVWQGIQLMH
                     KLTP"
     gene            6029..6829
                     /gene="metF"
                     /locus_tag="PWX02_000345"
     CDS             6029..6829
                     /gene="metF"
                     /locus_tag="PWX02_000345"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDL1728397.1"
                     /translation="MQQKWRMLWDSIDRLSPLKPKFVSVTYGANSGERDRTHSIVKSI
                     QEKTGIISAPHLTGIDATPEELKVIAKDYWDSGIRRIVALRGDIPKGYDKALFYASDL
                     VALLKSVADFDISVAAYPEVHPEAKSAQADLLNLKRKIDAGANHVITQFFFDVESYLR
                     FRDRCVSIGIDCEIVPGILPVTNFKQLQKMAAVTNVKIPTWMGKMYEGLDDDQTTRNL
                     VAASIAMDMVKVLSREGVNDFHFYTLNRSELTYAMCHTLGVRPDLTTA"
     gene            complement(6878..8038)
                     /locus_tag="PWX02_000346"
     CDS             complement(6878..8038)
                     /locus_tag="PWX02_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728398.1"
                     /translation="MKKSILLILSLFSASLFADVQLSPLPLPQIGKVSQFDLSEQGEL
                     VVINQQKQLWRVTQQAQKLADGVSTEIEPKAQYQRIALADQNGHFMLWTADKIYTSNI
                     PLAPKATMTALNFATIAVTKQNGQFKLARIETQGNQATITATAETEVLPDAHPLQIDF
                     DAETPNQGHIAVLAKPDDFTYQHGVLGDDVEAGEIQFLERHTLNPLAEKLTQQGRVFE
                     ANRWATFTENGKTKIVSVISGDGNGASTVVIGVKDGKLSIEAESEPLSTNRWQSPFIF
                     NRKLYAIQMPHLKGNLVEYSLKGNQLAERFITSGLSNHRYGDHETNLVATTETFALIP
                     QMGYQQVVVLDKTGKITELPTALPAGIKQTKADSNKAYLLLKNGEIWVAEEK"
     gene            complement(8098..9114)
                     /gene="galE"
                     /locus_tag="PWX02_000347"
     CDS             complement(8098..9114)
                     /gene="galE"
                     /locus_tag="PWX02_000347"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDL1728399.1"
                     /translation="MAILVTGGAGYIGSHTLVELLNENREIVVLDNLSNSSEVSLERV
                     KQITGKSVKFYQGDILDRDILRKIFAENQIESVIHFAGLKAVGESVREPLRYYQNNVT
                     GSIVLVEEMLKANVNTIVFSSSATVYGDPQIIPIVESCPVGGTTNPYGTSKYMVERIL
                     EDTVKAFPQLSAVVLRYFNPVGAHESGLIGEDPNGIPNNLMPFISQVAVGKLPQLSVF
                     GGDYNTHDGTGVRDYIHVVDLALGHLKALDKHQNDAGFHIYNLGTGTGYSVLDMVKAF
                     EAANGITIPYKVVDRRPGDIAVCYSAPQKALEQLGWKTERGLEQMMKDTWNWQKNNPN
                     GYKG"
     gene            complement(9202..9363)
                     /locus_tag="PWX02_000348"
     CDS             complement(9202..9363)
                     /locus_tag="PWX02_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HDL1728400.1"
                     /translation="MNETLLNNLVCPVSNEKLEWDKENNRLINPKLNIAYPIKNGIPE
                     LLPEAGVAL"
     gene            complement(9341..10342)
                     /gene="lpxK"
                     /locus_tag="PWX02_000349"
     CDS             complement(9341..10342)
                     /gene="lpxK"
                     /locus_tag="PWX02_000349"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HDL1728401.1"
                     /translation="MKIWQTKSLITWLLSPLSLLFWLISQARKRLYSLNILQSYRSPL
                     PVLVVGNISVGGNGKTPVVVWLVEQLQQRGVKVGVISRGYGGKAKTFPQLVTRESSAE
                     MVGDEPVLIVQRTNVPLAISPNRQQSIELLLSQFEPDIIITDDGLQHYALQRDMEWVV
                     VDGVRRFGNGFVLPAGGLRELPSRLNSVQAVICNGGTPQANEHLMTLEPSDAINLVTN
                     QRKPLSEFTQPCVAIAGIGHPPRFFTMLEKLGIPLAKTQGFADHQAFDKAQLAQFADS
                     QTPLLMTEKDAVKCHDFAQPNWWYVPVSATFSEKSTACLLAPILQKLGKEDERNTTQ"
     gene            complement(10344..10748)
                     /locus_tag="PWX02_000350"
     CDS             complement(10344..10748)
                     /locus_tag="PWX02_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1728402.1"
                     /translation="MKVHEKIKLLRENNHWTQDEMAQKLSMTTKGYAKIERGETISNL
                     PKIEQIAEIFNMDICELLSYGEEGKIYINNTDNNLTNSFISLGNGVEEIQRLQLIISH
                     KDELLAHKEEIIENQKRELALLRDLVETLKNK"
     gene            10883..11065
                     /locus_tag="PWX02_000351"
     CDS             10883..11065
                     /locus_tag="PWX02_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728403.1"
                     /translation="MNNQYNPLYGMLGILASAASVILPLYAAYKDYQFGENMLSLMDI
                     IIFPLGIVRGLMYLFG"
     gene            11069..11398
                     /locus_tag="PWX02_000352"
     CDS             11069..11398
                     /locus_tag="PWX02_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728404.1"
                     /translation="MKKYLFFAFMTLSNVAYSNLDEWYSASDKERREFIAKHFAEEIV
                     RSDRNTPYDYAMCTGRMFAKQLTRDEVKDYPLANTKPQYLTRDRRIEIMSNLPREILQ
                     NCAKYIQ"
     gene            complement(11434..12186)
                     /locus_tag="PWX02_000353"
     CDS             complement(11434..12186)
                     /locus_tag="PWX02_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDL1728405.1"
                     /translation="MAYKPPFTVTTKMLNLVVEISHKLGTLQLDFERNLHLRKNNRLR
                     SIQASLAIEANSLTIEQVTAIIKGKKVLGHPKDIREVKNAYDAYDEMMHYNPYSIKDF
                     LKAHKFLTADLVNDAGAFRSKDVGIYNFSGQLVHMGARPQFVENLINELFSWAKQDDT
                     PALIKSSVMHYEIEMIHPFSDGNGRMGRLWQNVLLAQWNPLFAWLPIETMVYENQQRY
                     YQMLSLADSQNDSTGFIEFMLEMILETLLKLK"
     gene            complement(12225..13472)
                     /gene="clpX"
                     /locus_tag="PWX02_000354"
     CDS             complement(12225..13472)
                     /gene="clpX"
                     /locus_tag="PWX02_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HDL1728406.1"
                     /translation="MAFEKEPHCSFCGKKRTEVDQLVEGTEGYICNECIEESYALLHG
                     EEEPLIDENQDDEGSFFTNVPTPHELHAHLDDYVIGQEHAKKVLSVAVYNHYKRLRSA
                     LSNHQTTNGVELGKSNILLIGPTGSGKTLLAETLARRLNVPFAVADATTLTQAGYVGE
                     DVENVIQKLLMKCDFEAEQAERGIVFIDEIDKITRKSESASITRDVSGEGVQQALLKL
                     LEGTVANINPQGGRKHPKGETIPVDTSKILFICGGAFAGLDKIVEARTNTQGGIGFAA
                     ALKKDKDRQDLTELFKQVEPEDLVKFGLIPELIGRLPVVTPLQELDEEALIQILTEPK
                     NAIIKQYQALFEMEGVELHFTKDALISIAQKAISRKTGARGLRSIVENLLLDTMYDLP
                     TLNAKKVTIGKGCVENGDKPKVE"
     gene            complement(13472..14065)
                     /gene="clpP"
                     /locus_tag="PWX02_000355"
     CDS             complement(13472..14065)
                     /gene="clpP"
                     /locus_tag="PWX02_000355"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDL1728407.1"
                     /translation="MALVPMVVEQTSKGERSYDIYSRLLKERIIFLNGQVEDNMANLI
                     VAQLLFLEAEDPEKDIYLYINSPGGVVTAGLAIYDTMNFIKPDVATLCTGQAASMGAF
                     LLSAGAKGKRFALPHARIMIHQPLGGARGQATDIQIQAEEILRLKATLTHRMAEHSGQ
                     SYEKVLADTERDNFMSAEEAAEYGLIDKVLTSRSEVA"
     gene            14177..14560
                     /locus_tag="PWX02_000356"
     CDS             14177..14560
                     /locus_tag="PWX02_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HDL1728408.1"
                     /translation="MTLTEIYQKFERCKSWEERYRLLIQLSRLLPKPSEQELAQIAEI
                     EGCESRLWFEFQAEPRKVVAYSDARLMQGILFIVSVALTEKTAEELKGFDLKTMFDEL
                     KISQNLTSTRLNGLGQLQKHIMMNL"
     gene            complement(14664..14740)
                     /locus_tag="PWX02_000357"
     tRNA            complement(14664..14740)
                     /locus_tag="PWX02_000357"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14704..14706),aa:Asp,seq:gtc)
     gene            complement(14829..15584)
                     /locus_tag="PWX02_000358"
     CDS             complement(14829..15584)
                     /locus_tag="PWX02_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL1728409.1"
                     /translation="MKKSIYDTPIFFERYQQLRQNPISMNEVVEKPTMFSLLPDLTGK
                     KVLDLGCGTGVHLAHYLTLGAEKVVGLDLSELMLQQAESDLSAHWQKGNDFSLYCLPM
                     ERLNEISEGDFDVVTSSFAFHYIADFSELLAKISQKMTACGTLIFSQEHPIVTCYREG
                     YRWEKNQEKQQVAYRLNYYREEGERDRSWFQQPFKTYHRTMATIVNNLIQGGFEIVQM
                     EEPMLADQPRWHNEFKDLQHRPPLLFIKAIKQK"
     gene            complement(15644..16639)
                     /gene="trpD"
                     /locus_tag="PWX02_000359"
     CDS             complement(15644..16639)
                     /gene="trpD"
                     /locus_tag="PWX02_000359"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HDL1728410.1"
                     /translation="MQALLDKLFANQALAQAESHRFFSEVIKGNVSNEQLAAALIALK
                     LRGETVDEIAGAAKAALENANPFSTPDYPFADIVGTGGDGHNTINISTTSAIVAATMG
                     YKIAKHGNRSVSSKTGSSDVLSHLGINVDVSPETARLALDETNICFLFAPTYHSGFKY
                     AAPVRQALKTRTIFNILGPLVNPAHAKRQLLGVYSPELVKVYAETVKALGYEHTIVVH
                     GAGLDEVAVHGETQVAEIQNGQIDYYTLTPEDFGIERHSLEDLKGGEPAENAEKATAI
                     LQGKGKLAHIDAVAANVALLMKTFGEDDLKANVQKVKETLESGKALETLRKLSTY"
     gene            complement(16657..17043)
                     /locus_tag="PWX02_000360"
     CDS             complement(16657..17043)
                     /locus_tag="PWX02_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005543439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HDL1728411.1"
                     /translation="MISVYGLKQTLADRRALIADVIFDCMQMSLGVPKQRHALRFDLL
                     DAENFYPPINRSQDFIVIEINLMQGRSEQLKKRLIKGLFSALHTKVGIVPMDVEITIK
                     EQPEHCWGFRGMTGNEATDLEYQVHR"
     gene            complement(17036..17626)
                     /locus_tag="PWX02_000361"
     CDS             complement(17036..17626)
                     /locus_tag="PWX02_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDL1728412.1"
                     /translation="MANILFIDNFDSFTYNLVDQFRELGHQVTIFRNDYPLEDFLAKA
                     ETTENCLVALSPGPGNPAEAGNLLEIIRRLKDKVPMIGICLGHQALIEAFGGKVVHTG
                     TVLHGKVSRIEHDNQAMFSGINNPMPVARYHSLMGDELPAEFIVNAKFEHIVMAIRHK
                     TLPICGFQFHPESILTVQGTKLLKQSVEWLLGEKND"
     gene            complement(17636..19192)
                     /gene="trpE"
                     /locus_tag="PWX02_000362"
     CDS             complement(17636..19192)
                     /gene="trpE"
                     /locus_tag="PWX02_000362"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDL1728413.1"
                     /translation="MPQLTITQTELPYFADTTRVYYALCGERPHTLLLDSAEIQSKNS
                     VKSLLFAKSAVHIFCEDQTVTFHALTPNGEAVLPLIANALSLLNPAPTCKFLDKNDRL
                     QAIFPTISNQLDEDSKLKTTTVFDGLRTVSQLFADSTSPIYLGGLFSYDLVTNFIPME
                     NISLQDDGLSCPDYTFYLAEQLVTIDHQTETAVLNTFCFEPNEQTNLNQQAVEFTQKL
                     ANLSHIELPLANQTATSEATANIEDEDFKAIIRKLKHHINIGDVFQIVPSRRFSLPCP
                     NTLASYKQLKINNPSPYMFYMQGENFTLFGASPESALKYSQANRQLEIYPIAGSRPRG
                     FDEQGNIDPELDSRLELELRLDQKELAEHLMLVDLARNDVARVSESGTRRVVDLMQID
                     RYSQIMHLVSRVIGTLRKDLDALHAYQACMNMGTLTGAPKIKAMQLIYQVEQQKRHSY
                     GGAVGYLTSDGNLDTCIVIRSAFVQNGIAYVQAGCGEVLDSDPQLEADETRHKARAVL
                     KAIGQVNAKA"
     gene            19334..19951
                     /locus_tag="PWX02_000363"
     CDS             19334..19951
                     /locus_tag="PWX02_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDL1728414.1"
                     /translation="MTQFFYIHPDNPQQRLINQAVEIIRNGGVIVYPTDSGYALGCAM
                     GDKHAMDRIVAIRKLPENHNFTLVCSDLSELSIYALVNNQAYRLIKNNTPNPYTFILP
                     ATKELPRRLMTKRKTVGIRVPDNAIALALISALGEPILSCSLMLPETEMTESDPDEIR
                     DHLQNRVDLIIHGGYLGQQPTTVVDLTDDVPVIIREGSGDPTPFN"
     gene            20097..21164
                     /gene="rluB"
                     /locus_tag="PWX02_000364"
     CDS             20097..21164
                     /gene="rluB"
                     /locus_tag="PWX02_000364"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HDL1728415.1"
                     /translation="MTTFQKKSTATFRADKRGESRPSFKKEEQKSERKFGKTSEKPPR
                     PTSKPLTQRIAKKVEKTTASEVASQEKKAKVVGEKLQKILARAGQGSRRELEEIIAAG
                     RVSVDGKMATLGDRVQVSSSTKIRIDGHLVNLMPAQKEICRVLMYYKPEGELCTRSDP
                     EGRATVFDRLPRLSGARWIAVGRLDINTSGLLLFTTDGELANRLMHPSREVEREYSVR
                     VFGNIDDAMLQRLRKGVQLEDGPANFKQIKVVGGTGLNQWFDVTLTEGRNREVRRLWE
                     SQGVEVSRLIRIRYGNIKLDKGLPRGGWEEMGLEQVNYLRELVGLPPETETKVEVGVN
                     RRRTNIRQIRKAVKQHQKYRG"
     gene            21229..23001
                     /locus_tag="PWX02_000365"
     CDS             21229..23001
                     /locus_tag="PWX02_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDL1728416.1"
                     /translation="MSWDIVATLAVTGIAVFLFATEKMRMDAVAILVLCSLVLLGQVD
                     TKSALSGFSNSATVTVTAMFVLAAGLQNSGALDSVGSLLAKVKSPWLFLLVLCGVNAA
                     VSPFVNNTAVVAVLIPIVIVAAQNIKMAPSKALIPLSFSSQMAGVCTLIGTSTNLLVN
                     AIAQKQGHSGFGMFEFAPLGIIFLVVGVAYLLVTSRFLLPESHLQLEDGEGFGKYVSE
                     LKVNKDSVLIGKSTFESGLNEEFNLFTIGVLRDGERLSTPSHQVLQKHDILLLRGESE
                     NLAKVREKYGLHHVVYGRRENDEDNLDEDLMVAEVMISPISRWIGGTIPILQQRWNKN
                     ATVLGIQRQSKVIRERLRTTAFKMGDILLLTLPKEDMETLRQDKDFIVLSSDLVKTEE
                     SWKSKFALGVMVLVVATAALGWVPISVSALIGAVAMCVAGCLTAEEAYQSIDWKIILV
                     LAGLLPLGEAMANSGAAQFIVDNTLGKVGEFGPLVVLAVLYLLTMVLTEFMSNAGTAV
                     LLTPIAVSTAKMLGVDASPFIIAVMFAAATSFMTPVGYQTNTMVYGAGGYKFTDFIKI
                     GLPLNVIYWILGIVLIPIFFPFNP"
     gene            complement(23091..27307)
                     /locus_tag="PWX02_000366"
                     /pseudo
     CDS             complement(23091..27307)
                     /locus_tag="PWX02_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249064.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
BASE COUNT         7661 a         5593 c         5917 g         8315 t
ORIGIN      
        1 ggcgagatct tcttattttg cacgtttagc agaatggact ttattagaac atttacttgt
       61 caaacagcct gaaaatattt cggtatgttc attcggtcaa tcattcggta caagcggtcg
      121 tttagatgca gaatattacc aacctaaatt tgcaagctta tttgcacaat tagccaattt
      181 caaaacgatg aaattaggtg agattgttaa tttacaaaaa tcggtagaac cgggctcgga
      241 agcttatcaa acagagggaa taccctttgt gcgtgtatcg gatttatcta aattcggcat
      301 cagccaaaca gataaatact tacatccgaa agattttggc aatgttgtaa gacctaaaaa
      361 agacagtata ttactgacta aagacggcac agtcggcatt gcttaccgtg ttccgcaaga
      421 tttgaatgtg attacatcag gggcaattgt gcatctagag cttaaaactg acgaagtttt
      481 acccgattat ttggcgttag cattgaattc tcctgctgta caattacaag cggaaagaga
      541 tgcgggaggc tctattatcc aacattggaa accgtctgaa attttagatg tagtgattcc
      601 ggtattacct aaaaatatcc agcaaaccat tagcgacaaa gtacaacaaa gttttgcatt
      661 gagagtagaa agtgaagttt tactggaaaa agctaaaata cttgtagaac aagaaataga
      721 aaatatgagg taataatgcc taatcataat caagatttga aaaaagagtg gtttaataaa
      781 gcaaggatag attaccactc tcttttttaa tgttatggct cagttgtaat agctggtata
      841 acttccatta ttccttaggt aaggatagaa cacatattga tagaataaaa tcggatacca
      901 gtaatcaaaa caaactttat aaagagtttg aaagaatatt cacctctgga aaaataaaag
      961 aaaaaactaa tttatggaat aatattgagc aacttcattt tgcattagtt caggcagagt
     1021 taaaatattc aggtagcaat attccaagtg aatattctaa atttaacttg gaaaatgtgc
     1081 ttattgattt tcctaacaag acaaattctg tagcatatca gaatctagtt attcacaacg
     1141 caaaaacgcg tgcaggaaaa ttaaaaacac aatatgcaaa tgctcacgat ttaggcaatt
     1201 tggttttagt agaggatatc caaaaaatat tttccgggtt attagaaata atttatcaag
     1261 ttcgttgcca tttggtacat ggtagcttaa gtccaacacc tgaaaaccac gaagttgtta
     1321 aatattgcta tttgattttg tgggattgtt taaaaggatt ttgtgattag ttattgtcaa
     1381 taaagctaca agcggtcaat tctcccaaaa ctttgcaaat ttccccgcaa aactgaccgc
     1441 ttgttctcct ctatccattc acctcaacca acttttctct ctacccctac tcaaaatcgg
     1501 ttaaacaatc tgttgaggta aatgttgtgt cggaatggga tttaatctta atgaaactga
     1561 aaaaatcttc tgccaagcaa atttccctaa aattggaagt tcatttccaa tttttcaatc
     1621 ccccacattt cccttttctc ccaatccatt tcagctttca ccccaaaagc cgttgcaata
     1681 ttttcggcag ttaagaccgc ttgtgtgttg ccgttggcga gtaaattacc gtttttaagc
     1741 agaatgattt catcgcaaaa acggtaggca aggggcagat gatgaatcac caccacgcag
     1801 gtctgggttg gggtgagggc tttgagctgc tgcataatgt cgatttggta gtaggggtcg
     1861 agggcggcaa tcggttcatc ggcaagcaag atcggggctt gtttcatcaa acaacgggcg
     1921 agttgcactc gggtctgttc accgccggaa aggcattggt aagtgttatc cagcaggtga
     1981 gaaatcgcca actgttcggc aaccgaacgc accttttgac gctcttgttc gggcgaaagc
     2041 ggtgcgggca agccgagtgc tatcacatca tataccgaaa gttgccacgc cacgttgagc
     2101 tgttgggcaa ggtaggcaaa ttgtcggctc cgttcaacgg cggaaagggc gtgcagtttg
     2161 caattttcca gccaaatttc accgcttgtc gggcgtagaa tgccggcaat cgctttgagc
     2221 aacgaggatt tccccgcccc gtttgccccc ataatgccga tcaatttgcc ttttggcaga
     2281 tagcaagaga ggtgttgaat accgtgagca agggtgaggt tttcaatctt gatcattgtg
     2341 ccaatctccg ttgctgggtg aataaaatcc aaatcaaaaa cggtgcgccc aaaatcgccg
     2401 tgagcgtgcc gatgtaaatg cgggcaaaaa acggcacata ttgcaccgct agatctgcaa
     2461 tcagcagtaa caacgccccg attaaagcgg aggtgaggta aagctgagac ggtggttttt
     2521 tcagccaaat acgggcaaga tgcggagcaa ttaagccgat aaaaccgata gtgccggttt
     2581 gtggaatagt tgctccgacc aacagcgcca cgccaatgct aacggtaaaa aaactgcgtt
     2641 gcaggttgat gcccattgtt gcagcggtct cttcgccgaa ggttagcagg tcgagataac
     2701 gccgttcccg atagaggcag acaatgccga gcaagatcaa ggggaacgcc caacatactg
     2761 cctctgcggt tgcccagact aatgagcctt gtaaccagcg gtagagttcc gccaacgccc
     2821 acgggctttc ggcattggaa agtagtaaag tcaccgctgc cccgagcaac acgtttaccg
     2881 ccacgccact taaaatcatc attgtagtgc cgtggcgaaa gctaatcagc cacaccagca
     2941 aaaacgcccc caacgcacct gctacaccgc caaacagtag ccagcccatc ggggctgaaa
     3001 aatagtagag cagaaacacg ctcaccgccg ttgcccccga actgctccca agcaaaccgg
     3061 ggctggcaag cggattttga aacacgccct gcatcgcatt accggcaagc gcaaggctgg
     3121 cgcccgtcag caccgccaag ccgatacggg gcagtcgaat ttcgcccagc accagccagc
     3181 gcagatcgct tagcacgcct tcggcatttt ttagcaaggc aaaatcgccc agttggtaaa
     3241 aggctgcgct gctaacaatc agcaacaaca agcttccaag cacaatgttt aatttcttca
     3301 ttcttctttc tctcatcaaa cgtgcagatt ataccatagc gccaaagtca agctgaaaac
     3361 gctatttttg cgatcgccat cgaaaaaata gcgtttgccg aatgtaaccc acgacaggct
     3421 ttgctatatt ccttcaatca gcctaagttt tgtatttaaa acattacaga ttgggggtag
     3481 aatgtaccat attgaacgca ctgaaatttt cgaccaatgg cttgaaaatt taaaagatcc
     3541 acttgctgtg gtgaccattg caagacgtat tgaacgtgcc gaaagcggaa attttggcga
     3601 taccaaatat atcgcagaag gaatttccga aatgagatta aacgtcagca aaggttatcg
     3661 cctttactat gcacaaaaag gtagcattat ttatatacta ctaaatggag gatataaagg
     3721 cacgccaagc tgatattgat aaagcataaa atgctatggg cagaaattaa acagcatatg
     3781 gcagaacagg agaaaaagaa tgaacgaatt atacgatgac ccgattccac aagccactcg
     3841 tgaaaaatat ggtatcaccg aatttgatgc cgccgaatac ctcaccaatg acgagctaat
     3901 cgccctttat ttggcggaaa cattgaaaga cggcacggac gaggagttta ttcaggcatt
     3961 gaataccgtg gcacgagcga aaggaatgaa tgagttagcg aaaaaagcag ggatcgggcg
     4021 tgaaagtttg tatcaaagcc tttccagcag taagccccgt tttgagacta tccgcaaaat
     4081 tattagcgca ttaaatttgg agttgtcggt cgttaaggcg taagtttgtg cattaattgt
     4141 atcccctgcc aaaccccgtg gtcgaaacag taggtgtatt tcatcggtag gacaagcggt
     4201 ggattttggc tcattttttg ccatattttt tgtaatgctg ggtgtttgag cagttcggct
     4261 tggttgttat agctttggtt atcggtgagg aacaataact gtttgggttg cgctaacagc
     4321 agtttttcca gcgagaaatt ctgtggcgtg aggtcgcttt ttaatggcga tagaccgaga
     4381 agatgcaaca acgtttgata ttgcggaaaa tgcggttcga tcatacccgt ttcgcttaga
     4441 attaaagttt cagcaagcgg ttgttttagc ggaatttttt gcaaacgtag ccgttccacc
     4501 agcttttccg ctttggcttg attttgggtt agctcgccca gttgtaaaat cagtgtgaaa
     4561 agctgttcgg gtgtttgcgg actgtcgttt aatgcgacta acttaaagcc aagctgtttc
     4621 agccgtgcgg tgagttgcgg atagaaggtt tcattcagta aaatggtgct gtcggcatag
     4681 ggcaacaggg cggtgagttc aggctcaacg gtgggcttgt ccgtgttcag tttatccaac
     4741 atcatcaacg gcttttggga atagggcgac atcgcccgaa tttgctgtgg ttcggcaagt
     4801 tccagcaaca gacggtcgct acacaaattt ggcgataaac ggtaagctcc tgaaatacgc
     4861 ccagtgatag aatttttaaa ctgtgaatta tcggtgtaac ggccactaat agaaaaactg
     4921 tgatcttgtt cggtaaataa gcgatattct agtgccgcac ttttttcgga taaggttttt
     4981 tcacgcaaat atttatcaga ggtatagcgt gtttttgcat aatctgtcag taaactgacc
     5041 gcttgtgtca cttctccttc acggtcaaag tttacatcta attgataatt cgcattaagt
     5101 tgtttttcgt tgctgtagcg agcatttgaa ccgaaatgtg ttgctttact attgaattgg
     5161 ctgatatttg cttggtgttt cagcagctct tgttcatttc cccaataacc gcctaattta
     5221 aaagtttgat tgcgagtacg ggaatattca ccgctaacgt ctgaattata gccatcaata
     5281 tgaacggttt gggaagattg agcggcaaga acttctaccc ctttgttacc gaggtcataa
     5341 cctaaacgta aagagccgtt atcacgatga aagccatctc gttcgcttgc accaccggtt
     5401 ttcacttctg agcctgtttc tgtggtatag ctgaaatggt ttttactcag tgcggaaatg
     5461 cctttggtac gatggctatc accgtgtaag gcatagtaga aaccgttgtg aaatccggaa
     5521 agtgttgcgc tggcatcacg ggtattattt gagcctaaac caagatcgac ctccgcattg
     5581 aatggtttct ctttgtataa accgctttta gtggtgatat aaactacacc acccatagca
     5641 ctgcttcccc aaagggcgga ttgctcgcca cgcaacactt cgatacgttc gatattgcta
     5701 atcggtaagc caccaaaatc gaaatttcca atattaatcg gattgacttt tacaccgtca
     5761 atcacaaccg cagtatggcg agattctgcc cctcgtaaaa ataagctagt aagagtacca
     5821 cgcccacctt gttgcccaat cgccacgccc ggcacagtct ttaacacatc actcacataa
     5881 gtcgcattgc gtgcggcaaa atctttttcg gtaagaaccg tcacgctgga agcggttttg
     5941 tcttggttga ttggggttgc ataggcggaa tagactgtta cggaatcaag ctgagtcatc
     6001 tcattttgtg caaaagctgt acttgctagt gcaacagaaa tggcgaatgc tgtgggattc
     6061 tattgatcgc ctaagtcctt taaaaccgaa atttgtttcg gtaacttatg gagcaaattc
     6121 tggtgagcga gatcgtacac acagtattgt gaaatcaatc caagaaaaaa cagggatcat
     6181 ttcagctccg cacttaacag gcattgatgc aacaccggaa gaattaaagg taattgccaa
     6241 agattattgg gatagtggta ttcgccgaat tgttgctttg cgtggcgata taccgaaagg
     6301 ttatgataaa gcactatttt acgcctcaga tttagtcgct ttattaaaat cagtagcaga
     6361 tttcgatatt tccgtggctg cctaccctga agttcaccct gaagcaaaat cagcacaggc
     6421 agatttactt aacttgaaac gtaaaatcga tgcgggtgca aaccacgtta ttacgcaatt
     6481 cttttttgat gtagagagtt atttacgttt tcgtgatcgc tgtgtttcta ttgggattga
     6541 ttgtgaaatt gtgccgggta ttctgccggt gacaaatttc aagcagttgc aaaaaatggc
     6601 agcagtgact aatgttaaga tcccgacttg gatgggtaaa atgtacgaag ggttagatga
     6661 tgatcaaacc accagaaact tagttgcagc aagtattgca atggatatgg tgaaagtgct
     6721 ttctcgtgaa ggggtgaatg atttccactt ctatacctta aatcgtagtg aactcactta
     6781 tgcaatgtgc catactttag gcgtaagacc tgatttaacg actgcataat cgaacattaa
     6841 ggctgtatag ctaaatgagc tatacctcct tatagtttta cttctcttca gccacccaaa
     6901 tctcgccatt ttttaataat aaataggctt tgttggaatc ggctttggtt tgtttaattc
     6961 cagctggcag ggcggttggc aattcagtga tcttgcctgt tttatccaac acaacaactt
     7021 gttgatagcc catttggggt atcagtgcga aagtctctgt tgtcgccact aaattggttt
     7081 cgtgatcacc atagcggtga ttgcttaagc cggaggtaat aaaacgttca gctaattggt
     7141 ttcctttcag gctgtattca accaaattgc cttttagatg tggcatttgg attgcataga
     7201 gcttgcggtt aaagataaac ggcgattgcc aacggttagt cgacagcggc tcactttcgg
     7261 cttctattga taattttccg tctttaacgc caatcacaac agtgctggcc ccattgccat
     7321 cgccagagat aacagaaacg atcttggttt ttccattttc tgtgaaagtc gcccagcggt
     7381 tggcttcaaa tactcgccct tgttgggtga gtttttctgc taaggggttt agggtatggc
     7441 gttctagaaa ttgaatttcg cctgcctcta catcatcgcc taatacaccg tgttgatatg
     7501 taaaatcatc aggctttgca agtacagcaa tatgcccttg gttaggtgtt tcggcatcaa
     7561 aatcaatttg cagagggtga gcatcaggca acacctcggt ttctgcggtg gcagtaatag
     7621 tggcttggtt tccttgcgtt tcaattctgg ctaacttaaa ttgtccgttt tgtttggtca
     7681 cggcaatggt ggcaaaatta agggcggtca ttgtagcttt cggggcaagg ggaatgttgg
     7741 aggtatagat tttatcagcc gtccacagca taaaatgccc gttttgatcc gctaacgcaa
     7801 ttcgttggta ttgagcttta ggttcaatct cggtggaaac tccatctgcc agtttttgag
     7861 cttgttgtgt gactctccat aattgttttt gttggttaat taccactaat tcgccttgtt
     7921 ctgaaaggtc aaattggctt actttgccaa tttggggaag tgggagtgga gaaagctgaa
     7981 catcagcaaa caaagaagcg gagaacaagc ttaagattaa cagaatagat tttttcatta
     8041 gcgttaccta aaaagaagat ttaaatgata aagggagatg ttatctccct ttacgcttta
     8101 gcctttataa ccattcgggt tatttttttg ccaattccaa gtgtctttca tcatttgttc
     8161 tagcccacgc tcggttttcc aacccagttg ctctaacgct ttttgtggag cggagtagca
     8221 aacggcaata tcgcccgggc ggcgatctac cactttgtat ggaatagtaa tgccatttgc
     8281 ggcttcaaag gcttttacca tatctagcac cgaataacct gttcctgtgc ctaaattgta
     8341 aatgtggaaa ccggcatcat tttggtgctt gtctaaggct tttaagtgac caagtgctaa
     8401 atctaccacg tggatataat cacgcacacc tgtgccatcg tgagtattat aatcgccacc
     8461 aaacaccgat aattgaggta atttgcccac agccacttgg ctgataaacg gcattaagtt
     8521 atttgggata ccgtttggat cttcaccgat taaaccgctt tcgtgagccc ctaccgggtt
     8581 gaagtaacgc aatactaccg cacttaattg tgggaaggct ttaacggtat cttcaagaat
     8641 gcgttcaacc atatatttgg acgtaccgta agggttggtt gtgccaccga cagggcaaga
     8701 ttccacaatc gggataatct gcggatcacc ataaacggtc gccgatgaac taaacacaat
     8761 ggtattcaca ttggctttta gcatctcttc taccaataca atcgagccgg ttacattgtt
     8821 ttgatagtaa cgtagcggtt ctctgacgct ttcgcctaca gcttttaaac cggcaaagtg
     8881 aattaccgat tcaatctgat tttccgcaaa aattttgcgt aaaatatcac gatctaaaat
     8941 atcgccttga tagaatttta cgctcttgcc ggtgatctgc ttcacacgct caagtgatac
     9001 ttcagaggaa ttagaaagat tatctaacac cacgatttca cgattttcgt ttaataattc
     9061 aactaatgta tgtgaaccga tgtagcctgc accacctgta actaaaattg ccattttatg
     9121 ttcctctata aaattaatac gaaccaaggt tattgttgct attttacccg atttaatcag
     9181 gcttgctaat atttattcag tttataatgc cactccggct tccggcagta attccggaat
     9241 gccgttttta atcgggtaag caatattgag cttagggtta attaatcggt tgttctcttt
     9301 gtcccattct aatttttcat tagagacagg gcagacgagg ttattgagta gtgtttcgtt
     9361 catcttcttt tcctagtttt tgtagaatcg gggctaacaa acaagcggtc gatttttctg
     9421 aaaatgttgc agaaaccggt acataccacc aatttggctg tgcaaaatcg tggcatttta
     9481 ccgcatcttt ctcggtcatt aataacggcg tttggctatc agcaaattga gccagctgag
     9541 ccttatcaaa ggcttgatga tcggcaaagc cttgagtttt ggcaagcgga atgcctaatt
     9601 tttctaacat cgtaaaaaag cgtggtgggt gaccgatacc ggcaatcgcc acacagggtt
     9661 gggtaaattc gcttaatggc ttgcgttgat tggtgactaa attaattgca tcagacggtt
     9721 caagcgtcat taaatgctcg ttagcttgcg gtgtgccgcc gttgcaaata accgcttgca
     9781 cggagtttaa acggctcggt aattcacgca atccgcccgc cggcagcaca aaaccgttac
     9841 cgaaacgcct tacgccatca accaccaccc attccatatc tcgctgtagg gcatagtgtt
     9901 gcaagccatc atcggtgatg atgatgtccg gctcaaactg acttaatagt aattcaatgc
     9961 tttgttggcg gtttggagaa atagccaaag gcacattggt tcgctgaaca atcagaaccg
    10021 gttcatcacc caccatttca gcagaacttt ctcgggtaac taattgcgga aacgttttcg
    10081 ctttaccgcc atagcctcgg gaaatcacac ctactttcac gccacgctgt tgcaattgct
    10141 ctaccagcca aaccaccact ggcgttttgc cgttgcctcc aaccgagata ttaccgacta
    10201 ccagcacagg caaaggcgaa cggtacgatt gcaaaatatt cagagaatat aaccgcttgc
    10261 gagcttgact aatcagccaa aacagcaacg aaagcggcga aagcagccaa gtgataaggg
    10321 attttgtttg ccagattttc atattattta tttttaagtg tttcaactaa atctcgtaat
    10381 aaggctaatt cacgtttttg gttttcgata atttcttctt tatgggctaa taattcatct
    10441 ttgtgggaga taataagttg taaacgttga atttcttcta ctccatttcc taatgagata
    10501 aatgaattag tgagattatt atctgtatta tttatgtaaa tttttccttc ttccccatag
    10561 gataataatt cgcaaatatc catattaaat atttctgcga tttgttctat tttcggtaaa
    10621 ttagagatag tttcaccacg ttctattttt gcataacctt tggttgtcat acttaatttt
    10681 tgagccattt catcttgtgt ccaatgatta ttttctcgca gtaatttgat tttttcgtgt
    10741 actttcatat aacacctata gattgtggga gttgggatat aacttgatgt ggtaaaggat
    10801 aaattttgtg gattgtagca gtttattgag caatttagaa tagagtaaat tttatcttaa
    10861 gctcacagga gaagaaacat tcatgaataa tcaatataat ccattatatg gaatgctagg
    10921 tattttagcg tcagctgcat cagtaatttt gcctctttat gcagcatata aagactatca
    10981 atttggggag aatatgttat ctcttatgga tataattatt ttcccgttag gtattgttcg
    11041 tggacttatg tatttatttg gatagattat gaaaaaatat ttattttttg cttttatgac
    11101 attaagtaat gttgcttatt ctaatttaga tgagtggtac tcagcttcag ataaagagcg
    11161 aagagaattt atcgcaaaac attttgcaga agaaatagta agaagtgata gaaatacacc
    11221 gtatgattat gctatgtgca ctggtagaat gtttgctaaa caactgactc gagatgaagt
    11281 caaagactac cctttggcaa acactaagcc gcaatattta acccgtgata gacggattga
    11341 gattatgtct aatttacctc gtgaaatatt acaaaattgt gctaaataca tacagtaatt
    11401 ttatgagtaa caccactttg gaattaagtg gtgttatttt aattttaata atgtctctaa
    11461 aatcatctcc aacataaact caataaagcc ggtagaatca ttttggctgt ctgcgagaga
    11521 aagcatttga taataacgtt gttgattttc gtaaaccatt gtttcaatcg gcaaccaagc
    11581 gaaaagtggg ttccattgtg ctaaaaggac attttgccat aatcgaccca ttctgccgtt
    11641 tccatcacta aaggggtgaa tcatttctat ttcatagtgc ataaccgagc ttttaatgag
    11701 tgcaggtgta tcatcttgtt tagcccaaga gaacaactca ttaatcaaat tctctacgaa
    11761 ttgaggacga gcccccatat gcacaagttg cccactgaaa ttataaatgc cgacatcttt
    11821 tgaacgaaaa gcccctgcgt catttactaa gtcagcagta agaaacttat gtgctttgag
    11881 aaaatcttta atcgagtaag gattatagtg catcatttca tcgtaagcgt cataggcatt
    11941 tttgacttca cgaatatctt tggggtgtcc gagaaccttt ttgcccttaa tgattgcggt
    12001 aacttgctca atcgttaatg aatttgcctc aatagctaaa gaggcttgaa ttgatctcaa
    12061 gcggttattt tttctaagat gtaaattacg ctcaaaatcc aattgtagcg ttcccaattt
    12121 atgagaaatt tctacaacca gattcagcat tttagtggta actgtaaaag gtggtttata
    12181 agccattttg caataatcct aaaaaattaa accgcttgtc attgttactc caccttcggc
    12241 ttatcgccgt tttcaacaca gcctttgccg atggtgactt ttttcgcatt gagggttggg
    12301 agatcgtaca ttgtgtcaag taataagttc tcgacaatag aacgtaaccc acgagccccg
    12361 gttttgcggc taatcgcttt ttgggcaatt gaaattaatg catctttggt gaagtgcaat
    12421 tcaacgcctt ccatttcgaa taaggcttga tattgcttga tgatcgcatt tttcggctcg
    12481 gttaagattt gaatcagagc ttcttcgtcc aattcttgca gcggagtcac caccggtaaa
    12541 cgcccgatta attcaggaat taagccgaat ttgactaaat cttccggttc aacttgtttg
    12601 aatagctcgg ttaaatcttg gcgatcttta tcttttttca atgccgctgc aaaaccgata
    12661 ccaccttgag tgttggtacg ggcttccacg attttatcta aaccggcaaa agcaccgccg
    12721 caaataaaga gaattttgga ggtgtccacc ggaatggttt cgcctttcgg gtgtttgcga
    12781 ccgccttgtg ggttaatgtt cgccaccgtg ccttcaagta gttttaataa ggcttgttga
    12841 acaccttcgc ccgatacatc acgggtaatg gatgcacttt ccgatttacg ggtgattttg
    12901 tcgatttcat caataaaaac aatgcctcgt tccgcttgtt cggcttcaaa atcgcatttc
    12961 attaataatt tttgaatcac gttttcgaca tcttctccca catagcctgc ttgggtaagg
    13021 gtagtagcat cagctaccgc aaaaggtacg tttaaacggc gggcaagggt ttctgccaac
    13081 aaggttttac cgctaccggt cggcccgatt aataaaatat tacttttacc taactctacg
    13141 ccattagtgg tttgatggtt tgacaatgcg cttcttaagc gtttgtagtg gttataaacg
    13201 gccaccgaaa gcactttttt cgcgtgttct tgaccgatga cataatcatc taaatgagcg
    13261 tgtaattcgt gaggtgtcgg cacattggtg aaaaaactgc cttcatcgtc ttggttttca
    13321 tcaattaacg gctcttcttc accgtgtaat aaggcgtagg attcctcgat acattcatta
    13381 caaatatagc cttctgtgcc ttcgactaat tgatcgactt cggtacgttt tttaccgcaa
    13441 aaactacagt ggggttcttt ttcaaatgcc attacgccac ctcactgcga gaagttaata
    13501 ctttgtcgat taagccatac tcagcggctt cttccgcaga cataaaatta tcacgctcgg
    13561 tatcggctaa cactttttca tagctctgac cgctgtgttc tgccatacgg tgggtaaggg
    13621 tagctttcaa gcgtaaaatt tcttccgctt gaatttgaat atcggttgct tgacctctcg
    13681 ctccacctaa aggttggtga atcataatac gagcgtgcgg cagagcaaag cgtttacctt
    13741 ttgccccggc tgaaagcaaa aatgccccca ttgaagccgc ttgccctgtg caaagggttg
    13801 ctacatcggg tttgatgaag ttcattgtat cgtaaattgc caagcctgct gttaccacgc
    13861 cacccggtga gttaatgtat aaatagatgt ctttttccgg atcttcggct tctaaaaaca
    13921 gaagttgtgc cacaatcagg tttgccatat tgtcttccac ctgaccgttt aaaaaaataa
    13981 tacgttcttt taataaacgt gaataaatgt cgtaagaacg ttcgcctttt gaggtctgtt
    14041 ccaccaccat tgggactaat gccattttgt tttcctctgt cgtaatacaa aatctcgcta
    14101 ttctagcaaa ttttcagaaa atcattaaca acaagcggtc agattgttaa aatcttttgc
    14161 aaatgatgag gaaattatga cattaacaga aatttaccaa aaatttgaac gttgtaaatc
    14221 gtgggaagaa agataccgct tgttaattca gctaagtcgc ttgttaccaa aaccaagtga
    14281 gcaagagttg gcacaaatag cggaaattga gggctgtgaa agccgtttat ggtttgaatt
    14341 tcaagctgag ccacgaaaag tagtggcata tagcgatgcc cgtttaatgc aggggatttt
    14401 atttattgtg agtgtagctt taactgaaaa aacggcagaa gagctgaaag gctttgactt
    14461 aaaaacgatg tttgatgagc taaaaatttc acaaaattta accagcacaa ggttaaatgg
    14521 gttgggtcag ttacagaaac atataatgat gaatttgtaa tttaccctct cccacaaggg
    14581 gcgagggtaa ataaaatccg caggatttta ttcgggagag gggaatataa aaaacctctg
    14641 atttctcaga ggcttaaatt tgttggcgga acggacggga ctcgaacccg cgaccccctg
    14701 cgtgacaggc aggtattcta accagctgaa ctaccgctcc gtactggtgc taaaataata
    14761 gtggtttcct caacattgag caaatagttt ttgcaaaata gtgtttaatt ggttgtaaaa
    14821 taaccacttt atttctgctt gatcgccttg ataaacagca acggcggacg gtgctgtaaa
    14881 tctttaaatt cattgtgcca ccgaggttgg tctgccagca ttggttcttc catttgcaca
    14941 atctcaaacc cgccttgaat aaggttattc acaatggttg ccattgtgcg gtggtaggtt
    15001 ttaaagggtt gttgaaacca acttctgtcc cgttcgccct cttcccgata atagttcaag
    15061 cggtaagcaa cttgttgttt ttcttggttt ttctcccaac gatagccttc tcggtaacag
    15121 gtgacaatcg ggtgttcttg cgagaaaatc agcgtgccac aagcagtcat tttttgcgaa
    15181 atttttgcaa gtagttcgga aaaatcagca atataatgaa acgcaaaaga gctggttact
    15241 acatcgaaat cgccctcgct gatctcattt aatcgctcca tcggcaggca gtaaagggaa
    15301 aaatcgttgc ctttttgcca atgagcagaa agatcgcttt ctgcctgttg tagcattaat
    15361 tcagataaat ccaaccccac cactttttct gcaccgagcg ttaaataatg ggctaaatga
    15421 actcctgtgc cacaacctaa atccagcact tttttgccgg ttaagtctgg cagtagagag
    15481 aacattgttg gcttttccac cacttcattc atacttattg gattttgtcg aagttgttgg
    15541 taacgctcaa agaaaatcgg agtgtcgtaa atgctttttt tcacttaata gcctttaaac
    15601 aaatataaaa taaggcgggt taaaacccgc cactacgata tgattagtaa gtcgataatt
    15661 ttcgcaaagt ttctaacgct tttccactct caagcgtttc ttttactttt tgcacattgg
    15721 cttttaaatc atcttctcca aaggttttca ttaataatgc gacatttgct gccaccgcat
    15781 caatatgagc cagtttacct tttccttgta aaatagcggt tgctttttcg gcattttctg
    15841 caggttcacc gcctttgaga tcttctaacg aatggcgttc aatgccgaaa tcttccggcg
    15901 ttaaggtgta atagtcgatc tgtccgtttt gaatttccgc aacctgcgtt tcgccgtgaa
    15961 cggcaacttc atctaaacct gcaccgtgta ccacaatagt gtgttcgtaa cctaaggctt
    16021 ttacggtttc agcatagact ttcactaact caggggagta cacaccgagt aattggcgtt
    16081 tagcgtgagc cgggtttacc aacggcccta aaatattgaa aatcgtgcgg gtttttaagg
    16141 cttggcgaac aggagcggca tatttgaatc cgctgtggta ggttggggca aacaaaaagc
    16201 agatgttggt ttcatctaaa gcaaggcgtg ccgtttcagg tgaaacgtca acattaatac
    16261 ctaaatggct taatacatcg cttgagccgg ttttacttga tacgctacgg ttgccgtgtt
    16321 tggcaatttt atagcccatt gtggcggcaa caatcgcact tgtggtagaa atgttgatgg
    16381 tattatgccc atcgccacct gtgccgacaa tatccgcaaa agggtaatca ggcgttgaga
    16441 acggattggc attttcaagt gcggcttttg cagcacctgc aatttcatca acggtttcgc
    16501 cacgcagttt tagagcgatt aaggcagcag caagttgttc atttgataca ttgcctttaa
    16561 taacttcact aaaaaaacgg tgagattccg cttgggctaa ggcttggttg gcgaataatt
    16621 tatctaaaag tgcttgcata gtctcttcct ttttaattag cgatgtacct gatattccaa
    16681 atcggttgcc tcgttgccgg tcattccacg aaagccccag caatgctctg gctgttcttt
    16741 aatggtgatt tcaacgtcca tcggcacaat gccgactttg gtgtgtaagg cactaaatag
    16801 tcctttaatt aagcgttttt tgagctgttc gctccgccct tgcatcaggt ttatttcaat
    16861 cacaataaaa tcttggctac ggttaattgg gggatagaaa ttttccgcat ccaataagtc
    16921 aaaacgcaaa gcgtggcgtt gttttggtac gccaaggctc atttgcatac agtcaaaaat
    16981 cacgtctgca atcagggctc tgcggtctgc taaggtttgt tttaatccat atacactaat
    17041 catttttttc tcctaataac cattccaccg actgcttaag taattttgtg ccttgcacgg
    17101 ttaaaatgga ttccgggtgg aactggaaac cgcaaatcgg taaggttttg tggcgaattg
    17161 ccatcacaat atgttcaaat ttcgcattaa cgataaattc tgccggtaat tcatcgccca
    17221 ttaatgagtg ataacgagcg acaggcattg gattgttaat gcctgaaaac atcgcttggt
    17281 tatcgtgttc aatacgagaa actttgccgt gcaatactgt gccggtatgc actactttgc
    17341 cgccaaaggc ctcaattaga gcttgatgtc ctaaacaaat gccaatcatc ggtactttgt
    17401 ctttcagacg gcgaataatt tccaataaat tgcctgcctc agccggatta cccgggccgg
    17461 gggaaagggc aaccaaacaa ttttcggtgg tttcagcttt ggctaaaaaa tcctcaagcg
    17521 gataatcatt gcggaaaatc gttacctgat gccctaattc acgaaattga tcgaccaagt
    17581 tataggtaaa agagtcgaaa ttatcaataa aaagaatatt tgccatagtt tgtccttatg
    17641 cttttgcatt cacctgccca atcgctttta acaccgcgcg tgctttatgg cgtgtttcat
    17701 ctgcctctaa ttgtggatcg gaatccagca cctcaccaca accagcttgc acataggcaa
    17761 tgccattttg aacaaaggcg gaacggatca cgatacaggt atctaaattg ccgtctgagg
    17821 ttaaatagcc caccgcaccg ccgtagctat ggcgtttttg ttgctcgact tggtagatca
    17881 gttgcattgc cttaattttc ggagcaccag ttagcgttcc catattcata caagcttgat
    17941 aagcgtgcag agcgtcaaga tcttttctaa gtgtgccgat cacacgagaa actaagtgca
    18001 taatttgcga atagcggtca atttgcatta aatcaaccac acggcgagtg ccggattcgc
    18061 tcacacgagc cacatcattg cgggcaagat cgactaacat taaatgttcg gcaagctctt
    18121 tttggtctaa acgcagttct aattccaaac gggaatctaa ttcaggatcg atattacctt
    18181 gttcatcaaa accgcgtggg cgagagccgg caatcgggta aatttccaac tggcggttgg
    18241 cttgcgaata tttcaacgca ctttcaggcg acgccccaaa cagagtgaaa ttttcgccct
    18301 gcatatagaa catatacggg cttggattgt tgattttgag ctgtttatag ctggcaagag
    18361 tattcgggca aggcaagctg aaacggcgag agggcacaat ttggaacaca tcaccgatat
    18421 taatatggtg tttgagtttg cggataatcg ctttgaaatc ttcatcttca atattggcgg
    18481 ttgcctcact cgttgcggtt tggttcgcta gaggcagttc aatatgtgac agatttgcaa
    18541 gtttttgcgt aaattcgacc gcttgttggt tcaaattggt ttgttcattc ggttcaaaac
    18601 agaaggtgtt tagcaccgca gtttcggttt ggtggtcgat tgtaaccagt tgctctgcta
    18661 aatagaaagt gtaatctggg caacttaagc catcatcttg caggctgata ttttccatcg
    18721 gaataaaatt tgttaccaaa tcgtagctaa ataatccgcc caaatagata ggcgatgtgg
    18781 aatcagcaaa cagttggctc accgttctta gcccgtcaaa cacggtggtg gttttgagct
    18841 tgctgtcttc atctaactgg ttgctgatgg ttgggaaaat cgcttgcaag cggtcgtttt
    18901 tgtctaaaaa tttgcaagtc ggagcagggt ttaacaggct caatgcgttt gcaatcaaag
    18961 gtaaaaccgc ttctccgttt ggggttaaag cgtggaaagt cacggtttga tcttcacaga
    19021 aaatatgcac tgccgatttc gcaaacagca aactttttac actgttttta ctttgaattt
    19081 ccgctgaatc taataatagc gtatgtgggc gttcgccgca taatgcgtaa taaacacggg
    19141 tcgtatcggc aaaatagggt aattctgttt gagtaatcgt taattgaggc atagcaaatt
    19201 cctattcaat ttttcgtact attaaactag tacattaaaa agaagtcaag caattattcc
    19261 agataattat gcgagcttgg gcgaacttgg tgtataatta gcccaatttt tagaaagtcg
    19321 aaggcttgag actatgacac aattttttta tatccacccc gacaacccac agcaacgctt
    19381 aattaaccaa gcggtagaga ttattcgaaa cggtggcgta attgtttacc ctaccgattc
    19441 aggctatgct ctcggttgtg caatgggcga taagcacgca atggatagaa ttgtagcaat
    19501 tcgtaaattg ccggaaaatc acaattttac cttagtttgt agtgatttat ccgagctttc
    19561 tatctacgcg ttggtcaata atcaagccta tcgcttaatt aagaataata ccccgaatcc
    19621 ttatactttt attctgccgg caactaagga acttcctcgc aggctgatga caaagcgtaa
    19681 aacagtgggg attcgtgtgc cggataatgc gatcgcattg gcgttaattt ccgccttagg
    19741 tgagccgatt ctttcttgct cgttaatgtt gccggagacg gaaatgaccg agtcagaccc
    19801 tgatgaaatt cgggatcatt tgcaaaatcg ggtggattta attatccacg gtggttattt
    19861 agggcagcag cctacaacgg tagttgactt aaccgatgac gtgccggtta ttattcgaga
    19921 aggatcgggc gatccaacac cgtttaatta aattgatgtt gtatgattaa tatatagaat
    19981 tatgcgtagg ggggctgtgc cagtccgtat taatatcgtt atttggacag acacaggacc
    20041 tgtccctaca atgaaaatca aattcagacg cttgtgaaag cgacaaaagg aaccttatga
    20101 caacttttca aaaaaaatca actgcgactt tccgtgcaga caaacgtggt gaaagccgtc
    20161 cctcatttaa aaaagaagag caaaaatctg aacgcaaatt tggtaaaacg tcagaaaaac
    20221 caccacgtcc aacttctaag ccattaacgc aaagaattgc aaaaaaagtg gaaaaaacga
    20281 ccgctagtga agtggcatct caagagaaaa aagccaaagt agtaggtgaa aagctacaaa
    20341 aaatcttagc gcgagcaggg caaggttctc gccgtgaatt agaagagatt atcgctgctg
    20401 gacgagtgag cgtggacggt aaaatggcta ctcttggtga tcgtgtgcag gttagctcat
    20461 cgactaaaat ccgcattgac ggacatttag ttaatttaat gccggcacaa aaagagatct
    20521 gccgtgtatt aatgtattac aaaccggaag gggaactttg cacccgttcc gatccggaag
    20581 gacgagccac cgtatttgac cgcttgcctc gtttaagtgg cgctcgttgg attgcggtcg
    20641 ggcggttaga tattaacacc tcaggcttat tattgttcac taccgatggc gaattagcca
    20701 accgtttaat gcacccaagc cgtgaagtcg aacgtgaata ttcggtgcgt gtgttcggca
    20761 acattgacga tgcgatgtta caacgcttac gcaaaggcgt tcagttggaa gacggaccgg
    20821 caaactttaa acaaattaag gttgtgggtg gcacaggctt gaaccaatgg tttgatgtga
    20881 ctttaaccga agggcgtaac cgtgaggttc gccgcctgtg ggaatcgcaa ggggtagaag
    20941 tcagccgttt gattcgtatc cgttacggca atatcaaatt agataaaggc ttacctcgtg
    21001 gcggctggga agaaatgggc ttagaacaag tcaattactt gcgtgagctg gtagggttac
    21061 cgccggagac tgaaactaaa gtggaagtgg gggtaaaccg ccgccgaacc aatatccgtc
    21121 aaattcgtaa agcggtaaaa caacatcaaa aatatcgtgg ctaaacacca tctttggggc
    21181 tgcattagtt ctgctaatgc agctattttc tttcaaaaag gagcatttat gtcttgggat
    21241 attgttgcaa cattggcggt cactgggatt gccgtatttt tatttgcaac tgaaaaaatg
    21301 cgaatggacg cagttgcgat tttagtgctg tgttcattag tattattagg gcaggttgat
    21361 accaaatctg ctctgagtgg attttcaaac tcagctactg taaccgtaac tgcaatgttt
    21421 gtattagctg ctggtttaca aaatagtggt gctttagata gtgtaggcag tttacttgct
    21481 aaggtaaaat ccccttggtt attcttgctt gtactctgtg gcgtaaatgc tgctgtttct
    21541 ccttttgtga ataatacggc agtggtcgca gtattaattc cgattgtgat tgtcgcagca
    21601 cagaatataa aaatggctcc ttccaaagcc cttattccgt tatcattttc ttctcaaatg
    21661 gcaggtgttt gtacactgat cggcacttcc actaatctat tagttaatgc aatcgctcaa
    21721 aagcaagggc attccggttt cggtatgttt gaatttgccc ctttggggat tattttttta
    21781 gtggttgggg ttgcctattt attagtgacc agccgtttct tattacccga atcacatttg
    21841 caacttgaag atggtgaggg gtttggaaaa tatgtttctg aacttaaagt aaataaagac
    21901 tctgtattaa ttggcaaaag tacatttgaa tcaggattaa atgaagaatt taatctattt
    21961 actattggtg ttttgcgtga tggggaaaga ttatcgacac cgagtcatca agtattgcaa
    22021 aaacatgata ttttattgtt gcggggtgaa tcggaaaatt tagcaaaagt acgagaaaaa
    22081 tatggattgc accacgttgt gtatggacgc cgtgagaatg atgaagataa tctggatgaa
    22141 gacttgatgg tggctgaagt gatgatttct cctatttctc gttggatagg agggactatt
    22201 cctattttac aacagcgttg gaataaaaat gcaacggtgt taggcattca acgccaaagt
    22261 aaagtaattc gtgagcgttt acgcaccact gcttttaaaa tgggggatat tttattactc
    22321 acattaccaa aagaggatat ggagacgtta cgccaagata aagactttat tgtgttatct
    22381 tctgatttag tcaaaacgga ggaatcgtgg aaaagtaaat ttgctttagg ggtaatggtt
    22441 ttggtggtgg caacagcagc cttaggctgg gttcctattt cagtgagtgc cttaattggt
    22501 gcagttgcga tgtgtgttgc tggttgttta acggctgaag aagcctatca atctattgac
    22561 tggaaaatca ttttagtctt agctggatta ctgccattag gtgaagcgat ggcaaatagt
    22621 ggggcggcgc aatttattgt tgataacacg cttggtaaag taggggagtt cgggccttta
    22681 gtggtgttag ctgtattata tttactgaca atggtgttaa ccgaatttat gagtaatgca
    22741 gggactgccg tgttgcttac gcctattgcg gtttctaccg ctaaaatgtt aggggtagat
    22801 gcttccccat tcattattgc cgtaatgttt gcggctgcga ccagctttat gacccctgtt
    22861 ggctatcaaa ccaacacgat ggtatacggt gcaggtggct ataaattcac agattttatt
    22921 aaaattggtt taccgctaaa tgtaatctat tggattttag ggatagtatt aattccgatt
    22981 ttcttccctt ttaatccata attgatagat aattatagaa aaaaggttta cagatcagaa
    23041 actctgtaaa ccttttttat tcagcaataa atcaattttc tattggaaaa ttaccattga
    23101 taacccatgc taatactacc accaaagtcg cctctggtat tgctgttccc agttaattta
    23161 tagataacct tcccgttatc tgaaatccga gaataaccta ctgcgattgc agcttcatta
    23221 cggtatgtat ctccagccgt tgctaacata cttttacctg gtagatatgc ttgcggtaaa
    23281 cctgcggttg cagttgcacc agcaatacca gcgcgtaaac gtttatcaac tttgtttact
    23341 tttgaatcaa tattattgat cttattattg atattttttt ctgttgcttt taattggcta
    23401 aagttaactg catcagtatc ttcagttcct gcttttacgt tagtcacttt tgttcctgct
    23461 gcattaatac ctgttttagt tatactcggt ccccctacaa tagttaatcc attagtatca
    23521 atggttgtat caccagtctt aacagtatcg gctacagcaa tgttagctgt tactgtttga
    23581 gctgtgacag aattaacttt aatatcttta gctaatttaa ccgttaatga accattacca
    23641 tttgaaataa ccccgatatt attatcagtt agttgtgatt cgttagatac cccacctttt
    23701 actgtaacag tagaacctaa tttacgttga gatggcgtgc cggtatcacc tgcaaatgtt
    23761 agtgggctgg ttactgcatc atttaacgca gtaattgcag agcctacatt attgaccgtt
    23821 ttactaactt catttgtagg agcgccatca accacagtat agcttggggc tgaaacagca
    23881 ccatttgcat ttacacttga attaccacct aaagcggtag caatagtatt tgccgtatcg
    23941 taaagttggc taccattaac tgcatctgct gagataggtg aaatatcacc atcagctaca
    24001 ttagttattt ttaatttgcc agcatcgaca cctgtcattg tgacacttgg gccattatta
    24061 atggttaagc cgttgttatt tagttttgaa ttaccaacag ctacggagcc ggcaggagtt
    24121 aaatcaatgt ctttcgctaa tttaactgtt aatgtgttag catctgttgc cactacaccg
    24181 atattattat cagtcaatat ggttgcatcg gccccacctt taacattcag agaagtacct
    24241 aatttacgaa caatttcagt accagcatca tctacaaaag taagtgctcc attacttaac
    24301 tcattcactt tcgtgtttaa accatcaatt tcggtataag cagcataaag ttgtgaaccg
    24361 ttgatagcat ccgttgatga gtccgaaata cgacctgctg caacattcgt taaagtacgt
    24421 tcattgccta cagaaccaat acttacagtt gagcttggcg tagtacctgc aaatgtgtat
    24481 gtattgccat ttagcgttgc actttttgtt gcaactgtgg ctactgttgc agagttagta
    24541 cctaatgcaa cagaaccttg gtgtgttgct tttgcatttt ggccaagagc cactgcagca
    24601 gttgttgttg cttgtgatga aacacctaat gcaatagaag ctacaccggc tgcatttgca
    24661 ctgttaccta aagcaatcgc agagctacct gcagcattgg catttctacc cacagaagtt
    24721 gaccatattc cacctgcatt agcagagtta cctaaagcgg ttgactgtgt acccgaagca
    24781 tttgcgttat agcctgcagc aagagaatct gcaccgattg aacttgcttc ataacctaca
    24841 gcggttgaat tttcaccggt agcttcagat ttagagccta atgcagttgc acgggtagct
    24901 gtcgctgttg aagctgtacc aatagctgtt gaggtatcag catttgcatt ggctgcagaa
    24961 ccaaccgcga gggcatttgt tgcatttgca ttcgcattgt agcctataga agttgcattc
    25021 ataccatcag ctgttgcgcg aggaccaact gcagttgtac gttcaccgtt tgcttctgct
    25081 tcagagccta atgcggttgc cagctcacca ttagctcttg ttgcaacgcc gatagccatt
    25141 gaattaacac cgctagagcc tgaattatca tagttagcac gttgaatgcc atcatctgaa
    25201 atactaacat aatgagttgc aaatttgctg atagcttgtt tgagttgact gacattgacc
    25261 gcatcagtat ctacatcgcc tgcggcaaca ttggcgattt tattaccgcc attatctaag
    25321 ccagatttag taagacttac cggtttgtct gcaacgccgt tagcgatggt gatacctgta
    25381 ttgttaacaa cggtatcacc tgttgttaat gaaccattag cgcctaagtt aactttttct
    25441 gctaatttta tggttaaagt actattgcca tcagctacaa caccaatatt attctcggtt
    25501 aatatgcctg tagatccgcc tttgatcttg actgttgaac ctagatggcg ctcaaaatta
    25561 gttcctgtat caccggcaaa ggttaacgga ctgatcaccg ctgtgtttaa tgcgcttagt
    25621 gctgcagaaa cagtagtgta tctcccttgt ttgccttctt tatctgcatc tggtgtgcca
    25681 ttcactaaag cgtaggttgg gaaagtgata tttccatttt cagtaacagc tgccccacca
    25741 cctaagatat ttgcggttgt tgcagcaaca ttacctatag cttgttgtgt taaatataat
    25801 tgtgaaccgt ttatggcatc tgtagagctt aaagatatac gtcctgcagc aacattggtg
    25861 atagttctct ctttgccttc cgccccaata cttactgtag caattggatt tgttccagca
    25921 aagtcactat atgtaatacc atttacagtt gcatttgtag ttggtatggc tatagctgta
    25981 actgagttgc tacctagcgt tatcgaatcg gtatgttcag cagtggcatt tcgacctata
    26041 gcggttgcat taaaggcgga agcatttgat gattctccga ttgcaattgt gcgttgattt
    26101 gttgctttag cgcttacccc tagtgcaaca gaagccgcgc ctgatgcagt agcacgattg
    26161 cctaaagcgg ttgcagaacc tccacttgca ttggcttcac gaccaaatgc agaagcataa
    26221 tcgctggttg cacccgcatg agtaccaaca gcagtagtta aatttgctgt tgcatttgca
    26281 taataaccta atgaggttgc accgtggcct gaagtcttcg cttcataacc aattgctgtt
    26341 gagttatcgc catttgcttg agattttgca ccaattgctg tggagtgaat agctgttgcg
    26401 ttagcttcag caccataggc ggagctaaca ataccagttg cattggcatg actacctaca
    26461 gcagttgctt ttcctattac cgtttctcct gttgctgctg tagttttgta attattaaca
    26521 ccatttgcaa gagcggaatt accgatggcg atattatcat cactggttac atttgtagta
    26581 ggtcctgcta gttttgcatt agcataagta ccaattgcaa tattgtggct accactagta
    26641 ccgtctccag cagtattacc aatagaaatc gtattgtttc ctgttacatt agagctagcc
    26701 gaataaccaa tacagcagta ttaccaatag aaatcgtatt gtttcctgtt acattagagc
    26761 tagccgaata accaatacca atattgtatt ctgtgctgtt agagtgattt aatccggcat
    26821 gatcgccaat aaaaacttca cgtaagccca ttgcggttgc attgtaccct actgctacgg
    26881 aacgtttatc ggcagtggct tgcccaccaa cagctgttga agcaaaacta gcgttagcaa
    26941 agcctcctaa tgcagttgaa aaatcggctg tcgcggttgt gttataccct aatgctgtag
    27001 aaactttacc gctatttcct tctaagcctg ttattgtgct tactagtgtt gagttaactt
    27061 tagatccatc accaattgcg atagatgttt gtgggttatt atctggtatg gtcccatagg
    27121 ttggtgaata ggttgcagca cttgcaaaac ctgcagaaag cgcaagactt agtgtgctga
    27181 gtttaataat attagtggtt agtgatgtat ttgttgcatt agatttatta gcacttcttg
    27241 ataatttaga agcaacaatc caagttcgtt ttgcatggtt ccaaacaatg cgataaattt
    27301 tattcataga actactcctg ttcttatttt ttaataattt taataatttt aatgatttga
    27361 gtattgccta gccaaatata tacaataggc tgaaaaatta tataggtgag gttttatggt
    27421 gtaaatagtg gctttttagg aataaatata gcaatttttt gatataaatg tattatttta
    27481 aatttt
//
LOCUS       DANJWC010000012        27307 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.012, whole genome shotgun sequence.
ACCESSION   DANJWC010000012 DANJWC010000000
VERSION     DANJWC010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 27307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27307
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.012"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(88..1023)
                     /gene="ftsX"
                     /locus_tag="PWX02_000367"
     CDS             complement(88..1023)
                     /gene="ftsX"
                     /locus_tag="PWX02_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HDL1728417.1"
                     /translation="MIRSFSKLGAQTNYSLRAVWQDLMQRKLGTFLTVLVIAVSLTIP
                     TVSYLLWKNTHEAATQFYPEPQLTVYLHKNLAEHDVNTVVDRIRAFETDKIESFNYIS
                     RQQSLDEFRSWSGFGEALDILDENPLPAVVTLKPKANFNNADTMVELRNGLQQIKGVQ
                     EVRLDNGWLEKLTALTWLIARIAVVCTLLMLLAVFLVIGNSVRTDVANSKAAIEVQQI
                     LGATDHFIARPFLYTGMIYGLFGSLLAVLFSAVTISYFTGVVKYVTDMFTVQFELNGL
                     DFSEAFFLVVLCVFIGWLSAKIATMRNIFRIGSRG"
     gene            complement(1033..1686)
                     /gene="ftsE"
                     /locus_tag="PWX02_000368"
     CDS             complement(1033..1686)
                     /gene="ftsE"
                     /locus_tag="PWX02_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDL1728418.1"
                     /translation="MIKFTNVNKVYKGGKPALQGIDFHLPAGGMAYITGHSGAGKSTL
                     LKLIMGIERANGGQILFNGHDITRLAHHELPFLRRQIGMVHQDYRLLTDRTILDNVAL
                     PLIILGMNQAIVEREARIALERVGLADKANYLPLHLSGGEQQRVDIARAIVHRPTLLL
                     ADEPTGNLDEKLSFEIFRLFEEFNQAGTTVLIATHDTNIISKRPKPCLVLEQGHLKF"
     gene            complement(1679..3175)
                     /gene="ftsY"
                     /locus_tag="PWX02_000369"
     CDS             complement(1679..3175)
                     /gene="ftsY"
                     /locus_tag="PWX02_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDL1728419.1"
                     /translation="MSEKKKGFWSWLGFGKKEEAEQKEIQQEQESIQPEQPVIVEPET
                     EKSFSEKLDELEDKIEEKVDQLEDYFEEKEEKIEQFAEQVEDTIEQKVEDFKEFVEEK
                     VEEAKAFFDDVEDKIEQTFEEQKGEQAVENLQNIATEQLDLNEQEQVTYQVEEAVAEE
                     VAETELVNNVQDTVEESTIQDEYQEKPSQGGFFSRLIQGLIKTKQNIGSGFRNFFRGK
                     KIDDELFEELEEQLLVADLGMPTTQKIINNLTQHATKQQLKDADLLYQQLKVELGEVL
                     KPVAQPLVLEAKKPYVILMVGVNGVGKTTTIGKLARKFQQEGKSVMLAAGDTFRAAAV
                     EQLQVWGERNNIPVVAQSTGSDSASVIFDAMQSAAAKGIDVLIADTAGRLQNKNNLMD
                     ELKKIVRVMKKYDETAPHEIMLTLDAGTGQNAISQAKLFNEAVGLTGITLTKLDGTAK
                     GGVIFAIADQFNIPIRFIGVGEKIEDLRPFNAEEFIEALFSHEDEQND"
     gene            3254..3844
                     /gene="rsmD"
                     /locus_tag="PWX02_000370"
     CDS             3254..3844
                     /gene="rsmD"
                     /locus_tag="PWX02_000370"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDL1728420.1"
                     /translation="MKKNRNSPQSVQQMGEVRVIAGLWRGRKLPVLNAEGLRPTTDRV
                     KETLFNWLMHDIAHSRCLDCFAGSGSLGIEALSRQAQAVVFLEKFANAANQLKKNLQA
                     LKSEQGSVIHTDTLAYLAQKNNGEPFNIVFIDPPFHQGFVPQVLNLLAENNWLAPNAI
                     LYVETEKNHPPLELPQGWQILKEKAAGMVVSRLIQL"
     gene            3928..4239
                     /locus_tag="PWX02_000371"
     CDS             3928..4239
                     /locus_tag="PWX02_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HDL1728421.1"
                     /translation="MKKLTPDEAIDIAYDIFLEMAGENLDPADILLFNLQFEERGAVE
                     MVETSEDWDQEIGTLIDPDAFAEVWVGLVNEQDEMDDVFARFLISHDEENREYHVVWK
                     E"
     gene            4261..4773
                     /gene="hpaC"
                     /locus_tag="PWX02_000372"
     CDS             4261..4773
                     /gene="hpaC"
                     /locus_tag="PWX02_000372"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HDL1728422.1"
                     /translation="MANIDTNQPIEASLKFREAMAHLSSAVSIVTTNGVAGKAGLTVS
                     SVCSVTDSPPTLLFCINKNSHVHDIIQQNGKVCVNVLSHEQEDLAKHFAAILESTMEE
                     RFGWDIWNNGFDDQPVLRSAISALQGNIVNTHAVGTHTIFVVEVSHIDVEPNHSLVYF
                     ARQFRSVNID"
     gene            4891..5763
                     /gene="tehB"
                     /locus_tag="PWX02_000373"
     CDS             4891..5763
                     /gene="tehB"
                     /locus_tag="PWX02_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HDL1728423.1"
                     /translation="MENLICYKQMPVWTKATLPQMFQEKHNTKVGTWAKLTILKGSLK
                     YYELTEEGEIIKEDIFDANHQRPMVEPQLWHKVEALSDDLECQLAFYCKPEDYFAKKY
                     NLTATHSEVLNAVNYTKGGKALDLGCGRGRNALYLNLLGFDVTAVDHNQESIDFLNYM
                     IEKEQAQNITTAVYDINEATIGSQNGEYDLIISTVVMMFLNRERIPAIIENMQKNTKV
                     GGYNLIVCAMSTDDYPCPMPFSFTFKEGELADYYQGWELVKYNEDLGHLHKTDENGNR
                     IQLRFATMLAKKIS"
     gene            5901..6938
                     /gene="purM"
                     /locus_tag="PWX02_000374"
     CDS             5901..6938
                     /gene="purM"
                     /locus_tag="PWX02_000374"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDL1728424.1"
                     /translation="MSNTQLSYKDAGVDIHAGNELVERIKPDVKRTRRPEVMGGLGGF
                     GALCALPTKYKEPILVSGTDGVGTKLRLAIDLNKHDTIGQDLVAMCVNDLVVQGAEPL
                     FFLDYYATGKLEVDVATNVVKGIADGCVISGCALVGGETAEMPGMYHEGDYDLAGFCV
                     GVVEKSKIIDGSTVKTGDALIALASSGPHSNGYSLIRKVLEVSRSNPATDLLEDKPLS
                     EHLLAPTKIYVKSILKLVEEVDVHAIAHLTGGGFWENIPRVLPESAKAVIDESSWQWP
                     AIFSWLQEKGNISRYEMYRTFNCGVGMVIALPERDVEKALTLLKESGENAWLIGKIED
                     LGTGSEQVEIR"
     gene            7016..7294
                     /locus_tag="PWX02_000375"
     CDS             7016..7294
                     /locus_tag="PWX02_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDL1728425.1"
                     /translation="MQLFWPIYFVLMNLAALYLMYSDKQKAIKKEWRVPEITLLSFCL
                     LGGAIGVYLGMKYFRHKTQHWYFHVAVVLSTFAWLIGLPYFYLALSSY"
     gene            7437..8864
                     /gene="aspA"
                     /locus_tag="PWX02_000376"
     CDS             7437..8864
                     /gene="aspA"
                     /locus_tag="PWX02_000376"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDL1728426.1"
                     /translation="MSDVRIEHDLLGEREVPNSAYWGIHTLRAIENFNISNVTISDVP
                     EFVRGMVMVKKATALANGELGAIPKKIAKAIVAACDEVLVKGRCLDQFPSDVYQGGAG
                     TSVNMNTNEVIANLALEILGEAKGKYDIVNPMDHVNASQSTNDAYPTGFRIAVYNSIT
                     KLINKITYLQTAFENKATEFSDVLKMGRTQLQDAVPMTLGQEFKAFAVLLDEEVRNLK
                     RTAELLLEVNLGATAIGTGLNTPEGYSTLAVKYLAEVTQLPCSLSENLIEATSDCGAY
                     VMVHGALKRTAVKLSKVCNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPEV
                     VNQVCFKVIGNDTTVTFAAEAGQLQLNVMEPVIGQAMFESIELLSNACINLRDKCIDG
                     ITANRDICQNYVFSSIGIVTYLNPFIGHHNGDIVGKICATTGKSVREVVLERGLLTEE
                     QLDDILSIENLMNPTYKAKRFTEEE"
     gene            complement(8939..10957)
                     /gene="rep"
                     /locus_tag="PWX02_000377"
     CDS             complement(8939..10957)
                     /gene="rep"
                     /locus_tag="PWX02_000377"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HDL1728427.1"
                     /translation="MKLNSQQQQAVEYTKGACLVLAGAGSGKTRVIINKIAHLIAHCG
                     YSPKQIAAVTFTNKAAREMRERVAHSIGKENSKGLTISTFHTLGFEILKREYKLLGFK
                     SGMTLFDEQDQIALLKHLLPENVTEDKDLLKALIATISNWKNDLLSPERVLTRVRDER
                     ERVFSHFYQLYQNQLKAYNALDFDDLIMLPVLLFRQFPEAKSRWQQKIRYLLVDEYQD
                     TNTSQYELIKLIVGNEPNFTVVGDDDQSIYSWRGAKPENMQRLREDFDLQVIKLEQNY
                     RSSQRILHCANILIDNNDHLFSKRLFSELGQGEKLQVIEAKNEEHEAERIVGELIAHR
                     FSKKTKYKDYAILYRGNHQSRLLEKLLMQNRIPYKISGGTSFFARAEIKDMMAYLRLV
                     VNQDDDAAFLRIVNTPKREIGAATLEKLGILANEKQVSLFEAIFDFELIQRLTPKPYQ
                     ALQDFARWIVEIADQADRSDPIEAVKDLLAKIQYEAYLYEMAANPKVAEMQSRNVQTL
                     FEWVQAMLEGDEIEPPMNLVQVVNRLTLRDMLERGEEDDEADQVQLMTLHASKGLEFP
                     HVFLIGMEEGILPHQTSLDEDNVEEERRLAYVGITRAQQTLTFSLCKERRQYGEIIKP
                     EPSRFLLELPQDDLEWESEKPRMTEQEKEAKSSANIANLMAMLKANKK"
     gene            11205..12320
                     /gene="potA"
                     /locus_tag="PWX02_000378"
     CDS             11205..12320
                     /gene="potA"
                     /locus_tag="PWX02_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HDL1728428.1"
                     /translation="MEKVEQKAIIELKNVTKSYGSKTILKNLNLTINDGEFLTILGPS
                     GCGKTTALRLIAGFEDLTEGSIILDGQDVSNVPAEQRPVNTVFQSYALFPHMTIFENV
                     AFGLRMQKVPNAEIEPRVMEALRMVRLEDRAQQKPAQLSGGQQQRIAIARAVVNKPKV
                     LLLDESLSALDYKLRKEMQNELKALQRQLGITFIFVTHDQEEALTMSDRIIVMNAGKI
                     AQDGTPREIYEEPSNLFVARFIGEINVFDATVIERVDATNVKANVEGRICNIKVEDLE
                     VQPNQKLKVLLRPEDIVIEELDENESSKAIIGHIIDRNYKGMTLESSVRLDHNGMNVL
                     VSEFFNEDDPNIDHEVGQKVALTWYEGWEVVLNDEGE"
     gene            12307..13170
                     /gene="potB"
                     /locus_tag="PWX02_000379"
     CDS             12307..13170
                     /gene="potB"
                     /locus_tag="PWX02_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005698572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HDL1728429.1"
                     /translation="MKANNKFQNGAVATIFGWLLLFVLVPNVLVLIISFLTENRQSQY
                     FVDFAFSFDAYKSLFNDTYAAVLWNSLYMAGIATFFCLIIGYPFAFFIAKMPERIRPF
                     LLFLVVLPFWTNSLIRIYGIKIFLGVKGFLNESLMALGIISEPMRLLNSEIAIIIGLV
                     YILLPFMILPLYSSIEKIDNRLLEAAKDLGANAFQRFIKVTIPLTMPGIVAGCLLVLL
                     PAMGMFYVADLLGGGKTPLVGNIIKSLFLNTNQFALGSAVSIALTILMALMLYVYYRA
                     NKLLNKKVELE"
     gene            13170..13937
                     /gene="potC"
                     /locus_tag="PWX02_000380"
     CDS             13170..13937
                     /gene="potC"
                     /locus_tag="PWX02_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HDL1728430.1"
                     /translation="MKKMFRNLFMLVVFAYLYIPIIILVINSFNEDRFGLNWKGFTWN
                     WYERLFSNDTLVQATLNSLTIAFFAATFSTILGALTSIALYRYRFRGKKLVGGMLFVV
                     MMSPDIVMAVSMLVLFMILGIKLGFISLLIAHITFCLPYVVIAISSRLSDFDGKMLEA
                     AKDLGASEVTILRKIILPLALPSIISGWLLSFTISLDDVIVSSFVTSPSYEVLPLKIF
                     SLVKTGVTPEVNALATIMIIFSLTLVVLSQLVVKKKV"
     gene            14128..15051
                     /gene="oppB"
                     /locus_tag="PWX02_000381"
     CDS             14128..15051
                     /gene="oppB"
                     /locus_tag="PWX02_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HDL1728431.1"
                     /translation="MLKFIFKRLLEAIPTLLILITFSFFLMRLAPGSPFTSEKAYPPK
                     VMANIEAKYHLNEPLYKQYFLYMENLSKGDFGPSFKYKDQSVNNLIASAFPVSIKLGI
                     TAFIFAVLIGLTAGTIAALKQNSKWDYLVMGFSMTGIIMPSFVFAPLLVLMFAIYLKW
                     LPAGGWNGGQLYYMVLPVLSLTISYVAGIARITRGSMIEVLHSNFIRTAKAKGLPMRK
                     IIFKHALRPALLPVITYLGPAFVGIITGSMVIESVFGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGTLTILFNAIVDILYAVIDPKIRYS"
     gene            15066..15965
                     /gene="oppC"
                     /locus_tag="PWX02_000382"
     CDS             15066..15965
                     /gene="oppC"
                     /locus_tag="PWX02_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HDL1728432.1"
                     /translation="MQNSSKNQPLVEVTSTIQGRSLWQDARRRFFRNKAAVASLIILC
                     CIILFITFAPMLMPFSYEDTDWNMMGIAPDFESQHYFGTDSSGRDLLVRVAIGGRISL
                     MVGIAGALIAVLIGTIYGAISGYFGGKVDMVMMRFLEILSSFPFMFFVILLVTFFGQN
                     IFLIFIAIGMVAWLNLARIVRGQTLSLKNKEFVEAAIVCGVSRRQIIWRHIVPNVLGL
                     VVVYASLEVPSLILFESFLSFLGLGTQEPMSSWGALLSDGAAQMETSPWLLAFPAFFL
                     CLTLFCFNFIGDGLRDALDPKDK"
     gene            15975..17570
                     /locus_tag="PWX02_000383"
     CDS             15975..17570
                     /locus_tag="PWX02_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1728433.1"
                     /translation="MELLAVNNLDVYLKTDEELVHAVRNVSFSIEKGQTLAIVGESGS
                     GKSVTSMAIMQLLPKNIVSFGERSSIIFEEQNLLTLSEKAMQTVRGDRIGMIFQEPMT
                     SLNPYMPIGKQVAETVQTHNPQISNAEAEKMVLETLQKVKIPDAEKKMICYPHEFSGG
                     QLQRIMIAMAIINKPDLLIADEPTTALDVTTQAEILDLMHDLQRDMGMAIILISHDLR
                     LVHKYSDLVCVMQNGEIIERGETETVFTNPQHPYTVELLTPIPTNLKGELPPNAPTLL
                     QGEHIKVDYILKRSLFGKPKKVFNALKDISLHLKTGETLGIVGESGSGKSTLGRAIMQ
                     ILDYRGNITFAGKTLANLNKAERQALKKDMQMVFQDPFNSLSPRLTVGEIIAEGLTVH
                     CPQMSKTERREKVMKMLEEVNLNPAMINRYPHEFSGGQRQRIAIARAIILEPKFVLLD
                     EPTSALDRSTQITVIELLNRLQKKYGLSYIFISHDLAVIKALSDRVIVMSQGEIVESG
                     DVTQIFENPQQEYTKRLILASNL"
     gene            17750..18145
                     /locus_tag="PWX02_000384"
     CDS             17750..18145
                     /locus_tag="PWX02_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL1728434.1"
                     /translation="MKKLMIFATTAMIVSNLAHAAGEQSDAREAHESTVKTSTVKYSC
                     QNGKKLSVKYGFNKQGIPTYAEAKLSGKKRFMPINLYTTDATGTNFGDENNFSLYGDP
                     MEFTNHRKASVNIQSPASEILYKGCTPQK"
     gene            complement(18355..20514)
                     /locus_tag="PWX02_000385"
     CDS             complement(18355..20514)
                     /locus_tag="PWX02_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HDL1728435.1"
                     /translation="MQIKQTVLAIAITPFISQLSLAQETKISSIELDTIEVNEQQENL
                     LKQQAVMSNEDLVRYSTDMGVPQTGRFGPQGFNIRGVEGNQVAVTVDGISQAASNNYA
                     TFSRYSYYNKSRPEIDTELMSDVTIEKGSSTKVGNGALGGSVNYKTKTVSDILMKGRQ
                     FGGMLKYTYNGQNKENIETAGIAADGQYADALVMYSHRQGHETQTTSKGPDIMGSGRG
                     LPNPYKNNQYSYLAKLGLKWNDENRLELKTSKQRSKNAGQELSYSTVTLRDFLDVQEI
                     ETYGVTYLYTPKNSILENIKVAVTKQDTEISGINDQITIARGTKTDNTKRAFNTKNVE
                     GSLGVTFKPVDLGNTEHKLSTELGYGVSDFSFDMQTYTAGITPTDQNMQSPAKTKSFH
                     ILMGDSILLTQNLSAHLNARYEQSKLHSDDNSLSDKTFNNWAGDIGLNYQLSPVWQIG
                     YKLSRGFRTPTASEMFFNREVQGRGASQTFLANTNLKPETSLGNEISLVGNGSLGSLS
                     VAAYHTHYRNLIDLATIGNNIYQSQNVNRAKIKGVDINGTLDLNTAWNAIPQGFELNG
                     GFGYARGKRNDGTDLLTVQPLKALFGIGYRAVNNDWAIHLNGTYTQGKKAKYAQQYAI
                     LANTGYLSYDKSLTTYPYLSNSATVFDLVAHKKFGERAIVKVGVYNLFNKKYWTWDSL
                     RTIGITSPGVANSITGEGLNRYLAPERYVMASVEFKF"
     gene            20632..21498
                     /locus_tag="PWX02_000386"
     CDS             20632..21498
                     /locus_tag="PWX02_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1728436.1"
                     /translation="MEYKMRTLLFSFIALSMCLNTTKAAEKIVSTAGYASEIVAALGK
                     ADKLVGVDTTSVKPQTIMEKKPKIGYRRQLSAEGILSLTPDLIILAPDAGPNAVIEQI
                     KASGIPLLHLGEKPSLAGIREDIQQIAKAIGAESEAKQLTETILADEKKLADIRSQHS
                     TDKEVLVLIDTASQGIFGLGKKSVGDHFLTLLNLNNSFNAEGNKPLANEALAASNADV
                     ILLASRNQAKESATISKLAESSPQYAQLTNTQAGKKGCVFAINILDALGFGPYTAQYA
                     HQILTTIQPCLK"
     gene            21509..22495
                     /locus_tag="PWX02_000387"
     CDS             21509..22495
                     /locus_tag="PWX02_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL1728437.1"
                     /translation="MKLHLTLIFTLIISLVVCTHLGAMSLPLSAWHCNNAECESAAYI
                     LWQIRLPRLLGTLFIGAALAISGATMQGLFRNPLVDPGIIGVSSGAALAAALYIVVIQ
                     PLLPESISYYALPIAAFLGGWLVTFMLYLLSQRNGQLHIAIMLLIGIALAAFSGALTG
                     ILIYIADDNQLRSLTFWSMGSLAGMNWQMVAMMAAVVFICSPLIWREHRALNALTLGE
                     EDAQYIGFDIAKVKRRLVFYVALLTGTSVACVGAIGFVGLVIPHLMRLLGGSNHRHLL
                     LNSLVCGAILLMIADTLARTLAAPAEIPIGIFTALFGAPFLAVMVWKSGKHI"
     gene            22495..23301
                     /locus_tag="PWX02_000388"
     CDS             22495..23301
                     /locus_tag="PWX02_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1728438.1"
                     /translation="MSNLLTCQNLSIVHQQKKLLEIQEIAFEQGKFTAIIGPNGAGKT
                     TLLKALSGNNAQTNSAVFYQHQRLTDLNGYALAQKRAMLSQQSQIAFPLKVEELVQLG
                     REPYRQSEFAKFNEKITAWAMETMSLTALKERSSIQLSGGEQHRAHIARVLAQLVPEP
                     NADLSGSWLLLDEPTNHLDIHHQYQLFSLLQKLKQQGLTIIAILHDPALAINQADHIL
                     MLKNGEKFAEMSAKAIAESCLLDELYEMKMGCRYCPHSGQYYLCPALAES"
     gene            complement(23322..23960)
                     /gene="purN"
                     /locus_tag="PWX02_000389"
     CDS             complement(23322..23960)
                     /gene="purN"
                     /locus_tag="PWX02_000389"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDL1728439.1"
                     /translation="MKKFVVLISGNGSNLQAMIDAQKSADTSGQICGVICNKADAYGL
                     IRAKQAGIPTFVFSRKDYQSNVEMDLAIAEQIEQLGAELIVLAGYMKILTPEFTQHFA
                     GKILNIHPSLLPKYPGLNTYQRAIEAGESEHGTTIHFVNEEVDAGAVVLQAKVPIYPE
                     DEIEDVMARVVEQEHRYYPLVIEWFCSGRLVSQHGKAYLDGKLLPACGYAED"
     gene            complement(24067..26265)
                     /locus_tag="PWX02_000390"
                     /pseudo
     CDS             complement(24067..26265)
                     /locus_tag="PWX02_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484758.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(26319..27155)
                     /locus_tag="PWX02_000391"
     CDS             complement(26319..27155)
                     /locus_tag="PWX02_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HDL1728440.1"
                     /translation="MLPRISSFLASLFMAFFLLGCSVSTQSLNEQNVDSIGQIIDLNT
                     NQTISFTELLSRLSSQDYVLVGEEHTQLIHHQIELYLFNQLTKKAKLHLIALEMLNVD
                     QQPAIDRIQFNRPVNLSATDLREAIQWQKWDWKMYQDLVVESLGSNSRVIATNLTDKE
                     VEILLNGAEPLKGSVSTSSEVKQKIAQLLLSMNHGMPYPMENMVAVQQFRDRRMAEKL
                     VKNALSTSLLIAGNHHVRKDLGVPLHIAEYDRTKKVAVLMLKTEREEITSSQADYLWV
                     TQ"
BASE COUNT         8012 a         5656 c         5288 g         8351 t
ORIGIN      
        1 agcaataata tattgcttat agtaaaaaac cagttcctag gaactggttt ttatctgcaa
       61 ttatttctct aaaaacaacc gcttgtatta gcctctacta ccaatacgga aaatatttcg
      121 cattgtcgca atttttgccg acagccagcc aataaacaca caaagcacca ctaagaagaa
      181 ggcttcgctg aaatctaatc cgttcagttc aaattgtacg gtaaacatat cagtcacata
      241 cttcactaca ccggtgaaat agctaatcgt taccgcacta aacagcaccg ctaacaggct
      301 gccaaacaag ccgtaaatca tacctgtgta taaaaacgga cgggcaataa agtgatcagt
      361 tgcccctaaa atttgttgga cttcaatcgc tgctttgcta ttggcaacat cggttcgcac
      421 actgtttcca atcactaaaa aaactgccaa cagcattaat aaggtacaca ccaccgcaat
      481 acgagcaatc agccaagtta gagcagttaa tttttccagc cagccgttat ctaaacgaac
      541 ctcctgcacc cctttaattt gttgcaaacc gttgcgtagc tccaccatcg tatcagcatt
      601 attaaaattt gctttcggtt ttaaggtaac gaccgcaggc aacgggtttt catctaaaat
      661 atctaacgct tcaccaaagc ctgaccaaga acgaaattcg tctaaacttt gttggcggga
      721 aatatagtta aatgactcaa ttttatccgt ctcaaaagca cgaatgcgat caacaacggt
      781 attcacatcg tgttctgcca aatttttgtg cagataaacg gttaattgtg gctcagggta
      841 aaattgtgtt gctgcctcgt gggtattctt ccaaagtaaa taactcacgg ttggaatcgt
      901 aagtgagacc gcaatcacca gcaccgtcag aaaagtgccg agcttgcgtt gcattaaatc
      961 ttgccaaacc gctcttaaag agtagttggt ttgagcacct aatttactaa atgaacgaat
     1021 cataatattt ccttaaaatt taagatgccc ttgctccagc actaaacacg gctttggacg
     1081 ctttgaaata atattggtat cgtgggtggc aatcagcacc gttgtgccgg cttgattaaa
     1141 ttcctcaaac aaacggaaaa tttcaaacga gagcttttca tctaagtttc cggttggctc
     1201 atcggctaat aacagcgttg ggcgatgaac aatggccctg gcaatatcga cacgttgttg
     1261 ctcaccgccg gaaaggtgca acggcagata gttcgcttta tctgccaagc caacacgctc
     1321 aagggcaatg cgggcttcac gctccacaat cgcttggttc attcccaaaa taatcaatgg
     1381 taatgcgaca ttatccaaaa tggttcggtc ggttaataag cgataatctt gatgcaccat
     1441 cccgatttgg cggcgtaaaa acggcaattc atgatgagca agtcgggtaa tatcgtgtcc
     1501 gttaaacaaa atttgcccgc cattggcacg ctcaatcccc ataattaatt ttaatagcgt
     1561 gcttttcccc gcaccggaat gcccggtaat atatgccatt ccaccggcag gtaggtggaa
     1621 atcaatgccc tgtaaggctg gctttccgcc cttataaacc ttgttcacat tagtgaattt
     1681 aatcattctg ttcgtcctcg tggctaaata aggcttcaat aaattcttct gcattaaacg
     1741 ggcgtagatc ttcaattttc tcgcccaccc caataaagcg gatcggaata ttgaattgat
     1801 cggcaatcgc aaaaatcacg ccaccttttg ccgtaccgtc taatttagtt aaggtgatac
     1861 cggttaaacc cactgcctca ttaaacaatt tcgcttggct gatcgcattt tgcccagtgc
     1921 cggcatcaag cgtaagcata atttcgtgcg gtgcggtttc atcatatttt ttcatcaccc
     1981 gcacaatttt cttcaactcg tccatcaggt tatttttgtt ttgcaaacgc cctgcagtat
     2041 cagcgattag cacgtcaatc ccttttgccg cagcggattg catcgcatca aaaatcaccg
     2101 aggcagaatc cgaaccggtg ctttgtgcca ccaccggaat gttatttcgc tctccccaaa
     2161 cctgtagctg ttcaactgcc gccgctcgga acgtgtctcc tgctgctaac attaccgatt
     2221 tgccttcttg ttggaattta cgggcaagtt tgccaatggt agtggttttc cccacgccat
     2281 tcacaccaac cattaaaatg acatacggtt ttttcgcttc caacactaac ggttgagcca
     2341 ccggttttag cacttcaccc agttccactt tcaactgttg atacaacaaa tctgcatctt
     2401 ttaattgttg cttggtagca tgttgtgtta aattgttgat gattttttgt gtggtcggca
     2461 tacctaaatc cgccacaagc aactgctctt ccagctcttc aaataactca tcatcaattt
     2521 tcttgccacg gaagaaatta cggaagcccg aaccaatatt ctgcttggtt ttaatcaagc
     2581 cttgaattaa acggctgaag aaaccgcctt gactcggttt ttcttgatat tcatcttgga
     2641 tagtagattc ttcaactgtg tcttgaacat tatttactaa ttcagtttca gcaacttctt
     2701 cggccacagc ctcttcgact tgataagtaa cttgctcttg ttcgttgaga tctaactgct
     2761 cagttgcaat attttgaaga ttttcgaccg cttgttcgcc tttttgttct tcaaatgttt
     2821 gttcgatttt atcttcaaca tcatcaaaga acgctttcgc ctcttcaact ttttcttcaa
     2881 caaactcttt gaaatcttcg actttttgtt caatggtatc ttctacttgc tcagcaaatt
     2941 gctcaatttt ttcttctttt tcctcaaaat agtcttctaa ctgatctacc ttttcttcaa
     3001 ttttatcttc cagctcatct aatttttcag aaaacgactt ctcagtttcc ggttcaacaa
     3061 taacaggctg ttccggttga atactttctt gttcttgttg aatctctttc tgttcagctt
     3121 cttctttctt accaaatcca agccaagacc aaaacccttt tttcttctct gacatatttt
     3181 tctctcattt ctcgtaaaaa tagactaaac ttacatagtc tatcaaaaaa tcccttattt
     3241 tcctttagtg agtatgaaaa aaaatcgaaa ttccccccaa tctgttcagc aaatgggcga
     3301 agtacgggtt atcgccggac tgtggcgagg cagaaaatta cctgtactaa atgccgaggg
     3361 gcttcgcccg acaaccgata gagtaaaaga aacgctgttt aactggttaa tgcacgacat
     3421 tgctcactca cgctgtcttg attgctttgc aggcagtggc tctctgggca ttgaagccct
     3481 ttcccgccaa gcacaagcgg tcgttttttt ggaaaaattt gcaaatgctg ccaaccagtt
     3541 aaagaaaaat ctccaagcat taaaatccga acaaggtagc gttattcaca ctgacaccct
     3601 cgcctattta gcacaaaaaa ataacggtga gccatttaac atcgtattta tcgatccgcc
     3661 ttttcatcaa gggtttgtac ctcaagtgct aaacttgctg gcagaaaata actggctcgc
     3721 cccgaatgcg attttgtatg tggaaaccga gaaaaatcac ccgccattag agctaccgca
     3781 aggctggcaa attttaaagg aaaaagcagc cggaatggtg gtaagccgtc tgattcagct
     3841 ataatctttt tgttttttat aactaaaaaa tataaaataa aacttatcta tctaacattt
     3901 tattaacact caaattagaa taacgaaatg aaaaaattaa cccctgatga agccattgat
     3961 attgcctacg atattttctt agaaatggca ggcgaaaatt tagatcctgc cgatatttta
     4021 ctgttcaacc tccaatttga agagcgtggt gcagtggaaa tggtcgaaac ttctgaagac
     4081 tgggatcaag aaatcgggac tttaattgac cctgatgcct ttgccgaagt ttgggttggc
     4141 ttagtcaatg aacaagatga aatggacgat gtattcgccc gtttcttaat ttcccacgat
     4201 gaagaaaacc gtgaatatca cgttgtttgg aaagaataag aaaaataaaa aggagaaaaa
     4261 atggctaata tcgacactaa tcaaccaata gaagcctcgc ttaaatttcg tgaagcaatg
     4321 gctcacttat cttctgcggt gagcattgtg acaaccaatg gcgtagccgg caaagcaggg
     4381 ctgacggttt cgtctgtttg ttccgttacc gattccccac caacgctctt attttgcatt
     4441 aataaaaaca gccacgttca cgatattatc caacaaaacg gcaaagtatg cgttaatgtg
     4501 ttaagccacg aacaagaaga cttagcaaaa cattttgccg ccattttaga aagtacaatg
     4561 gaagaacgct ttggttggga tatttggaac aacggctttg acgatcagcc tgtgttacgc
     4621 agtgctattt ctgctttaca aggcaatatt gttaatacgc acgcagtggg tacgcatact
     4681 atttttgtgg tagaagtgag ccatattgac gtagagccga accacagttt agtttatttt
     4741 gcccgccaat ttagaagcgt gaacattgac taaaatatat ttaacagaaa tttgatcaaa
     4801 tccgactttt ttagtcggat tttttatttc ctgtttttaa aacagtgcta aaattttgcc
     4861 gtttatgttt gactgacaaa aggaaacaaa atggaaaatt taatttgcta caaacaaatg
     4921 ccggtgtgga caaaagcaac cttaccacaa atgttccaag aaaagcacaa caccaaagtc
     4981 ggaacttggg caaaactcac cattctcaaa ggttctctca aatattatga attaaccgaa
     5041 gagggagaaa ttatcaagga agatattttt gatgcaaacc accaacgccc aatggttgag
     5101 cctcaacttt ggcataaggt tgaagctctt tctgacgatt tagaatgcca gttggctttc
     5161 tattgcaagc cggaagatta ttttgctaaa aaatataatc tgaccgctac ccattcagaa
     5221 gtgctaaatg ccgtcaatta taccaaaggt ggcaaagcgt tagatttagg ttgcgggcga
     5281 ggacgcaatg cactttatct gaatttactg ggttttgatg tcactgctgt tgatcataat
     5341 caagaaagca ttgattttct caactatatg atcgaaaaag aacaagctca aaacattact
     5401 accgctgttt atgatattaa cgaggcaact atcggctcgc aaaacggtga atatgacctg
     5461 attatttcca ctgttgtaat gatgttctta aaccgtgaac gcattcctgc cattattgag
     5521 aatatgcaga aaaacactaa agtcggcggt tataacttaa tcgtatgtgc aatgagtacc
     5581 gatgactacc cttgcccgat gccgttctcc tttaccttca aagaaggcga gctcgccgat
     5641 tattatcaag gttgggaatt agtcaaatat aacgaagatt taggacattt acacaaaact
     5701 gatgaaaacg gcaaccgcat tcaactacgt tttgcgacaa tgttggcgaa aaaaatcagc
     5761 taaacttaac cgcttgcagg gggaattagc ttccccctaa ttcatcaaat ccattctatt
     5821 tattttcact ttttcattcc gcaaacgttt gcttgtgcta taatggcttc gttttttagt
     5881 catttactgt tgaggttaaa gtgagcaaca cacaactaag ttacaaagat gccggcgttg
     5941 atattcacgc aggaaacgag ttagtcgaac gaatcaagcc tgatgttaaa cgcacccgtc
     6001 gccctgaagt aatgggcgga ttaggtggtt ttggtgcttt atgtgcttta ccgacaaaat
     6061 ataaagagcc gattttagtt tccggtaccg atggcgtcgg cacaaaatta cgcttagcca
     6121 ttgacttaaa caaacacgat actatcgggc aagatttggt agcgatgtgt gtgaatgacc
     6181 ttgtcgtgca aggtgctgaa ccgcttttct tcttagatta ttacgccacc ggtaaattag
     6241 aggttgatgt agccaccaat gtcgtcaaag gcattgcaga cggttgcgtg atttcaggtt
     6301 gtgctttagt gggaggtgaa acggctgaaa tgccgggaat gtaccacgaa ggcgattacg
     6361 atttagccgg attctgtgtc ggtgtggtag aaaaatctaa aatcattgac ggttcaacgg
     6421 tgaaaaccgg tgatgcctta attgcattag cgtccagtgg cccgcattcc aacggctatt
     6481 cgttaattcg taaagtattg gaagtcagcc gatccaatcc tgcaaccgat ttattggaag
     6541 acaaaccctt aagcgaacac ttattagccc caactaaaat ttatgtaaaa tcaatcctaa
     6601 aattagttga agaagttgat gttcacgcta tcgctcactt aaccggtggc ggtttctggg
     6661 aaaatattcc acgcgtattg ccggaatctg ccaaagcggt gattgatgaa tcgagctggc
     6721 aatggccggc gatatttagc tggttacagg aaaaaggcaa tatcagccgt tatgaaatgt
     6781 accgtacttt caactgcggt gtcggaatgg tgatagcctt acctgagaga gatgtcgaaa
     6841 aagcccttac tcttcttaaa gagagtggtg aaaatgcgtg gcttatcggt aaaattgaag
     6901 acttaggcac cggtagcgag caagttgaaa tccgctaatt tgcaaaataa atgctaaaat
     6961 tgaccgcttg tagtaactga tctgcaagcg gttaaatttt tattgaggaa tgcaaatgca
     7021 actattttgg cctatctact ttgtcttaat gaacctagct gccttatatt taatgtatag
     7081 tgacaaacaa aaagcgatca aaaaagaatg gcgagtgcca gaaattactc tcctctcttt
     7141 ttgcctctta ggtggggcta tcggtgttta cctaggtatg aaatactttc gccataaaac
     7201 ccaacattgg tatttccacg tcgctgttgt gctttctact tttgcttggc tgattggctt
     7261 gccttacttt tatttagctt tatcatcata ttaatttatc attacttttt ttaatccgaa
     7321 agcccaattt atatcctagt ccgctcacaa aaaatttgat ttagatcgca ttatttgtct
     7381 attaaaatcg ttattatcta ccaaaatcga ttgtcattta ttttaaggag atgaatatgt
     7441 cagatgtgcg tattgaacac gatttacttg gtgaaagaga agtacctaat agtgcttatt
     7501 ggggcattca tacattaaga gcgattgaga attttaatat ctcaaatgtc accatttctg
     7561 atgtacctga atttgtgcgt ggcatggtaa tggtaaaaaa agctactgcg ttggcaaatg
     7621 gtgaattggg tgcaatcccg aaaaaaattg ccaaagctat tgtggcagcc tgtgatgaag
     7681 tattagtgaa aggacgctgt ctcgatcaat tcccttcaga tgtttatcaa ggtggagccg
     7741 gcacatcggt gaatatgaat accaatgaag tgattgccaa ccttgcatta gaaattttgg
     7801 gcgaggcaaa aggtaaatat gatattgtca atccaatgga tcacgttaat gcaagccaat
     7861 ccaccaatga tgcctatcca accggtttcc gcatcgcggt ttataacagt atcactaaat
     7921 taatcaataa aatcacttac ctacaaactg catttgaaaa taaagccact gagttttccg
     7981 atgtactaaa aatgggaaga acgcagttac aagatgccgt gccaatgacg ctcggtcagg
     8041 aatttaaagc ttttgccgta ttattagatg aagaggttcg taaccttaaa cggacggcag
     8101 aattgcttct tgaagtgaat ttaggcgcaa ccgctatcgg tactggtctg aatacgccag
     8161 agggttactc tactcttgca gtcaaatatt tagccgaagt cacgcaactg ccttgctcac
     8221 tgtctgaaaa tctcattgag gcaacatctg actgtggtgc ttatgtaatg gttcacggtg
     8281 cattaaaacg caccgccgtc aaactttcta aagtgtgtaa tgacttacga ttactttcat
     8341 caggcccacg tgccggctta aatgaaatta atcttccgga attacaagcc ggctcatcta
     8401 ttatgccggc aaaagtaaac cctgttgtac ctgaagtcgt aaaccaagtg tgctttaaag
     8461 tgattggtaa cgacaccacc gttacttttg cagcagaggc cggtcaatta cagcttaacg
     8521 taatggagcc ggttatcggg caagcaatgt ttgaatctat cgagctgctt tccaatgcat
     8581 gtattaatct acgtgataaa tgtattgacg gtattaccgc taaccgtgac atctgccaaa
     8641 actatgtgtt cagctctatt ggtattgtga cctacctcaa tccatttatc ggccaccata
     8701 atggtgatat tgtcggcaaa atttgtgcaa ccaccggcaa aagtgtacga gaagttgtgc
     8761 ttgagcgagg cttactcacc gaagaacaac tcgatgatat tctctcgatt gagaacttaa
     8821 tgaatccgac ttataaagcc aaacgcttta ccgaagagga ataatcattg tttacaagcg
     8881 gtctgattct ggcaaaaaat tgcaaatttt taccaaaatc agaccgcttg ttatcccatt
     8941 atttcttatt tgctttcagc atcgccatta aatttgcaat atttgcagaa gattttgcct
     9001 ctttttcctg ttcggtcatt cttggctttt cactttccca ctccaaatca tcttgtggca
     9061 attccagtaa aaaccggctc ggctcaggtt tgatgatttc gccatattgg cgacgttctt
     9121 tacacagtga aaacgtgagc gtttgttggg ctcgggtaat gccgacataa gccaaacgcc
     9181 gctcttcttc tacattgtcc tcatcaaggc tggtttggtg tggcagaatc ccctcttcca
     9241 taccgattaa gaaaacgtgc ggaaattcca accctttcga ggcgtgaaga gtcattagtt
     9301 gaacttgatc ggcttcatcg tcctcttcgc cacgttcgag catatctcgc aaggttaaac
     9361 ggtttaccac ttgcaccaaa ttcatcggag gctctatttc atcgccctct aacatcgctt
     9421 gcacccattc aaacaaggtc tgcacattac ggctttgcat ttccgctact tttgggttcg
     9481 ccgccatttc atacaaatag gcttcatact gaattttcgc caacagatct tttactgctt
     9541 caatcggatc ggatctatcg gcttgatcgg caatttccac aatccagcgg gcaaaatcct
     9601 gtaaggcttg gtaaggcttt ggcgtgaggc gttgtatgag ttcaaaatca aaaatcgcct
     9661 caaacaaact gacctgcttt tcattagcaa gtatgcctaa tttttctagc gttgccgccc
     9721 caatttcccg tttcggcgta ttcacaatac gcaagaaagc tgcatcatcg tcttgattca
     9781 ctaccaaccg cagatacgcc atcatatctt tgatttccgc tcgagcaaaa aatgacgtac
     9841 cacccgaaat tttatacgga atgcggtttt gcattagcaa tttttccaac aagcgggatt
     9901 gatggttgcc tcgatacaaa attgcataat ctttgtattt ggtctttttc gagaaacgat
     9961 gggcaatcag ctcgcccaca atgcgttccg cttcgtgttc ctcatttttt gcctcaatga
    10021 cctgtaattt ttccccttgc cccagttctg aaaacagccg cttgctaaac aagtgatcgt
    10081 tattatcaat cagaatattg gcacaatgca gaatccgctg gctggaacgg taattttgct
    10141 caagtttaat cacttgcaaa tcaaaatctt cccgtaaccg ttgcatattt tcaggcttcg
    10201 ccccacgcca agagtaaatt gattggtcat catcgcccac gaccgtaaag tttggctcgt
    10261 tgccgacaat cagcttaatt aactcatatt ggctggtgtt ggtgtcttga tattcatcaa
    10321 ctaacaaata gcgaattttc tgttgccaac gagattttgc ttcgggaaat tggcggaaca
    10381 gcaacaccgg caacataatc aaatcatcaa aatccaacgc attataagcc ttaagttgat
    10441 tctggtagag ctgataaaaa tgtgaaaaca ctcgctcacg ttcatcacgc acacgagtaa
    10501 gtaccctttc aggcgaaagc aaatcgtttt tccagttaga aatagtggca attaaggctt
    10561 tcagcagatc cttatcttcc gtcacatttt caggcaataa atgtttcagc aaagcaattt
    10621 gatcctgctc atcaaacaag gtcatacccg atttaaaacc gagcaattta tattcgcgtt
    10681 ttaaaatttc aaacccaagt gtgtgaaaag tcgaaatggt taaacctttg ctgttttctt
    10741 tgccgataga atgagccacc cgttcacgca tttcccgagc agctttgttg gtaaaggtta
    10801 ctgctgcaat ctgcttaggc gaatagccac aatgtgcaat taaatgtgcg attttattaa
    10861 taatcacccg agtcttaccc gagccggcac cagccagcac aaggcaagcc cctttggtat
    10921 attctactgc ttgttgttgt tgagaattga gtttcattga atctattcaa aagtaatttt
    10981 gacaactatt ttaacggaaa gaaaacaagc ggtcgaattt tcaggaaaat ttgcaaaaaa
    11041 ctttgaaaat ccgaccgctt gttcatcaga gaaaaatccc caaaacgcaa atttttcctt
    11101 tcaatcttgc ccctttagct atatcattat aagttgattt tttgggggca aacgcctgat
    11161 tttataactc cttttctttt taatttaagg gaaaaaaaca gtagatggaa aaagtagaac
    11221 agaaagctat cattgagctt aaaaatgtaa caaaaagcta cggtagtaaa acgattctta
    11281 aaaatttaaa tttaaccatt aacgatggtg aatttttaac aattttaggc ccttccggtt
    11341 gtggtaaaac aactgcgtta cgtttaattg ccggttttga agatttaact gaaggttcaa
    11401 ttattcttga tgggcaagat gtttctaacg ttccggcgga gcaacgcccg gtaaacaccg
    11461 tattccaaag ctatgccctt ttcccacata tgacgatttt tgaaaacgtg gctttcgggc
    11521 tgcgtatgca aaaagtgcca aatgctgaaa ttgagccacg tgtgatggaa gcattacgaa
    11581 tggtgcgttt agaagaccgc gctcaacaaa aaccggctca gctttccggt ggtcagcaac
    11641 aacgtattgc aattgcccgt gcggtggtaa ataaaccgaa agtattgttg ttagacgaat
    11701 cgctttctgc gttagattat aaattgcgta aagagatgca aaatgaatta aaagcattgc
    11761 aacgtcaatt aggcattacc tttatttttg tgacccacga tcaggaagaa gccttaacca
    11821 tgtctgatcg tattatcgta atgaacgctg gtaaaatcgc tcaagacggt acaccacgtg
    11881 agatttacga agagccaagt aacttatttg tagctcgttt catcggcgaa attaacgttt
    11941 ttgatgcaac tgtaattgag cgtgttgatg caactaatgt aaaagcaaac gtagaaggtc
    12001 gcatttgcaa tatcaaagtg gaagatttag aggttcagcc gaaccaaaaa ttaaaagtat
    12061 tgttacgccc tgaagatatt gtgattgagg agttagacga aaacgaaagc tcgaaagcaa
    12121 ttatcggtca tattatcgac cgtaactaca aaggtatgac attagagtct agcgtgagat
    12181 tagatcataa cggtatgaat gtattggtta gcgagttctt caatgaagac gatccgaaca
    12241 ttgaccacga agtgggacaa aaagttgctc ttacttggta tgaaggctgg gaggttgtgt
    12301 taaacgatga aggcgaataa caaattccaa aatggtgcgg tcgcaactat tttcggttgg
    12361 ttattacttt tcgtattagt gccgaacgta ctcgttttaa tcatcagctt cttaacagaa
    12421 aaccgtcaaa gtcagtattt tgtggacttt gcatttagtt ttgatgctta taaatcactg
    12481 tttaatgaca cctatgcagc agtattgtgg aactcgctat atatggcggg cattgccacc
    12541 tttttctgtt tgatcatcgg ctatcccttt gcatttttta ttgccaaaat gccggaaaga
    12601 attcgtccgt ttttactgtt tttagtagtt ctaccgtttt ggactaactc actgattcgt
    12661 atttatggca ttaagatttt cttaggcgta aaaggctttt taaatgaaag cttgatggct
    12721 ttaggtatca tcagcgagcc aatgcgttta ttaaactcag aaattgcgat tattattggt
    12781 ttagtttaca ttctattacc ttttatgatt ctaccgcttt attcatcaat cgagaaaatt
    12841 gataaccgct tactagaagc tgcaaaagac ttaggtgcaa acgccttcca gcgttttatt
    12901 aaagtgacta ttccattaac aatgccgggc attgtcgcag gttgtttatt agtattactt
    12961 ccggcaatgg gtatgttcta tgtggcagat ttattaggcg gcggtaaaac accgttagta
    13021 ggtaacatta ttaagagctt gttcttaaat accaaccaat ttgcattagg ttcagcagta
    13081 agtattgcat taactatctt aatggcatta atgctttatg tttactatcg tgcaaacaaa
    13141 ttattaaata aaaaggtgga gcttgaataa tgaaaaagat gtttagaaac ctttttatgt
    13201 tggttgtttt tgcatatctc tatattccaa ttatcatttt agtgatcaac tcctttaatg
    13261 aagaccgctt tggattaaat tggaaaggct ttacttggaa ttggtatgag cgtttattca
    13321 gtaatgatac gcttgtacaa gcaacattga actcattaac tattgcgttc tttgcggcaa
    13381 cattctcaac cattttaggg gcattaacct ctattgcgct ttaccgttac cgtttccgtg
    13441 gtaaaaagct agtgggcgga atgttattcg tggtaatgat gtcgcctgat atcgtaatgg
    13501 cggtatctat gctcgtatta tttatgatct taggtatcaa gctaggcttt atttcgctat
    13561 taattgcaca cattactttc tgtttgcctt atgtggtaat tgcaatttcc tcacgcctca
    13621 gcgattttga cggcaaaatg ctagaagctg caaaagacct aggtgcaagt gaagtgacta
    13681 ttttgcgtaa aattatttta ccactggcat taccttcgat tatttcaggc tggttgttaa
    13741 gctttacgat ttcgttagat gacgttattg tttcatcttt cgtgacaagc ccaagctacg
    13801 aagttttacc actgaaaatt ttctcattgg ttaaaacagg tgtaacacct gaagtgaatg
    13861 cactagcaac tattatgatt attttctcat taacattagt ggtattaagc cagttagttg
    13921 tgaagaaaaa agtctaaaaa tcgtatacat aaatcggcac tttaaagagt attaaagtgc
    13981 cgattttttt acaaaaataa gctaaaaaaa caaccgctta agaataatta attcttataa
    14041 aaatagccat aactaatcaa tatctttaca aagccgttaa tttacgctat taattgccta
    14101 ccaatataac taaaaagagt ggtgcttatg ctgaagttta tttttaaacg tcttcttgag
    14161 gcgattccaa ccctactaat tctgattacc ttttcctttt tcctgatgcg gcttgccccg
    14221 ggtagcccat ttaccagtga gaaagcctat ccgccgaaag taatggcaaa tatcgaggca
    14281 aaatatcacc tgaatgaacc gctgtataag cagtattttc tttatatgga aaatctgtca
    14341 aaaggcgatt tcggtccatc ttttaaatat aaagatcaat cggtaaataa tctgattgct
    14401 tctgcgtttc ctgtttcgat aaaactgggc attactgcct ttatttttgc ggtgcttatc
    14461 ggtttaaccg caggaactat cgctgcactt aagcaaaata gtaaatggga ttatttagtg
    14521 atgggctttt caatgacggg tattattatg ccgagctttg tctttgctcc gctgttggta
    14581 ctcatgtttg cgatttatct caaatggttg cctgccggtg gttggaacgg tggtcagctc
    14641 tactatatgg ttttacctgt tctctcttta accattagct atgttgccgg cattgcccgt
    14701 attactcgtg gttcaatgat tgaggtgttg cactctaatt ttattcgcac cgctaaggca
    14761 aaaggcttgc caatgcgtaa aattattttc aaacacgctt tgcgtccggc attgttgcct
    14821 gtgatcactt atttaggccc ggcttttgtt ggcattatta ccggctcgat ggtaattgaa
    14881 agcgtgtttg gcttaccggg aatcgggcag ttgttcgtaa acggagcatt aaatcgtgac
    14941 tattcattag tattaagcct aaccattttg gtaggtacgc tcaccatttt atttaatgcg
    15001 attgttgata ttttatatgc ggtcatcgac ccgaaaattc gttattcata ggagtaaagc
    15061 ggaaaatgca aaattcttca aaaaatcaac cgcttgttga ggttacctca accattcaag
    15121 gacgaagttt atggcaagat gcacgccgcc gttttttccg aaataaggcg gctgtggcaa
    15181 gtttgattat tctttgttgt attattcttt ttattacctt tgccccaatg ctaatgccgt
    15241 ttagctatga agataccgat tggaatatga tggggattgc tccggatttt gaatctcaac
    15301 actatttcgg tactgattct tcagggcgtg atttgctggt tcgagttgca atcggtggac
    15361 gcatttcgtt aatggtaggg attgccggtg cattaattgc ggtgcttatc ggtacaatct
    15421 atggagcaat ttccggttat ttcggcggta aagtcgatat ggtgatgatg cgttttctgg
    15481 aaattttaag ctcattccca tttatgttct tcgtaatttt gctggttacg ttcttcgggc
    15541 agaatatttt cctgattttc attgcaatcg gtatggtagc gtggctgaac cttgcccgta
    15601 ttgtacgtgg tcaaactctt tcgcttaaaa ataaagagtt tgtggaagca gcgattgtgt
    15661 gtggcgtttc ccgccgtcaa attatttggc gacacattgt gcctaacgtt ttaggcttag
    15721 tggtggttta tgcctccctt gaagtaccat ctctgatttt gtttgaatcg ttcttaagtt
    15781 tcttagggct tggtacacaa gagccgatga gcagttgggg ggcattatta agcgacggag
    15841 cggcacaaat ggaaacctcg ccttggctac tggctttccc tgcctttttc ttatgtttaa
    15901 cactgttttg ttttaatttt attggtgatg gcttgcgtga tgccctcgat ccaaaagata
    15961 aatagaggtt tagaatggaa ttattagcag taaataatct cgatgtttat ttaaaaacgg
    16021 acgaagaatt agttcacgca gtgcgtaatg tctcgttctc gattgaaaaa gggcaaaccc
    16081 ttgcgattgt gggggaatcg ggttcgggta aatccgttac ttcaatggca attatgcagt
    16141 tattgcctaa aaatatcgtc tcattcggtg aacgctcaag catcattttt gaagaacaaa
    16201 acttgcttac tctatctgaa aaagcaatgc aaacagttcg aggtgatcgc atcggtatga
    16261 ttttccaaga gccaatgacc tcactcaatc cttatatgcc gattggcaag caggttgctg
    16321 agacggttca aacccataat ccgcaaatca gcaatgccga agcggaaaaa atggtgttgg
    16381 aaactttgca aaaagtaaaa attcccgatg cggaaaagaa aatgatttgc tatccgcacg
    16441 agttttccgg cggtcagcta caacgaatta tgattgcaat ggcaattatc aacaaacccg
    16501 atttattgat tgccgatgag ccgaccaccg ccctggatgt cacaacccaa gcggaaattt
    16561 tggatttaat gcacgactta cagcgtgata tgggaatggc gattatctta atttcccacg
    16621 atttacgctt ggtgcataaa tacagtgact tagtctgcgt aatgcaaaac ggcgaaatta
    16681 ttgagcgagg cgaaacggaa actgtgttca ccaatccgca acatccttat acggtggagc
    16741 tacttacccc gattccaact aatttaaaag gggaattacc gccaaatgca ccaaccttgc
    16801 ttcagggtga gcatattaag gtagattata ttttaaaacg ctcgctattc ggtaagccga
    16861 aaaaggtgtt taatgcgtta aaagatattt cattgcactt gaaaaccggc gaaacgctcg
    16921 gcattgtagg cgaatccggc tccgggaaat ctacccttgg gcgtgccatt atgcagattt
    16981 tagattatcg cggcaatatt acatttgcag gtaagacact ggctaatctg aataaagccg
    17041 aacgtcaagc cctgaaaaaa gatatgcaaa tggtgttcca agacccgttc aactcacttt
    17101 ctcctcgctt aacagttggg gaaattattg ctgagggctt aaccgtacat tgccctcaaa
    17161 tgagcaaaac cgaacggcgg gaaaaagtaa tgaaaatgtt ggaagaggtt aatctcaacc
    17221 ctgcaatgat taaccgctat ccgcacgaat tttcgggcgg acaacgccag cggattgcga
    17281 ttgcaagggc aatcatttta gagccgaaat ttgtattgct tgatgagcca acctcagcac
    17341 ttgaccgctc tactcagatc actgtaattg aattgcttaa ccgcttacag aaaaaatacg
    17401 gcttaagtta tattttcatc agccacgatt tggcggtcat caaggcactt agcgaccgag
    17461 tgattgtaat gagccaaggt gagattgtag aaagcggtga tgtcactcaa atttttgaaa
    17521 atccacaaca agaatacacc aagcggttga ttttagcctc taatttgtaa ccccttccac
    17581 aaggtcttat tctaccaaaa aacaagacct tgtcatttta tgctagcaaa acattgtaat
    17641 tttgatattt tatttcaatt ttttgaaaaa atatggaact ttatataaaa ctcactttct
    17701 aaacaatacg ttatctgaac taagataacg cttatttaaa ggagaatata tgaaaaaatt
    17761 gatgatattc gcaacaactg ccatgattgt aagcaattta gcccatgcag caggtgaaca
    17821 aagtgatgca agagaagccc acgaaagcac cgtaaaaacc tcaactgtta agtatagctg
    17881 ccaaaacggc aaaaaattaa gcgtgaaata cggcttcaat aaacaaggca ttccaaccta
    17941 tgctgaggca aaattaagcg gtaaaaaacg ttttatgccg atcaacctat acacgacaga
    18001 tgcaaccggc actaactttg gtgacgaaaa caatttcagc ctttacggtg atccaatgga
    18061 atttaccaac caccgtaaag catcagtaaa tatccaaagc cccgcaagtg aaattttata
    18121 taaaggttgc acacctcaaa aatagataaa aagtaaaatg agtccgctct gataagtggc
    18181 tttttaatag ctgtttattg cagataggaa agcattttgt ttttatctac tatccagaaa
    18241 caagcggtta aatttgttta attttttaca aaaaatgggc caaaattaac cgcttgttta
    18301 ttactataaa gaaataagta cgtaaaaatt aactcttaca tttaatgctt atctctaaaa
    18361 tttaaactcc actgatgcca ttacatagcg ctctggagca aggtaacgat ttaagccttc
    18421 gccagttata ctgttggcaa ctccaggact agttatacca atagttctta aactatccca
    18481 agtccaatat tttttattaa agagattata aaccccaact ttaacaatcg ctctttctcc
    18541 aaactttttg tgtgcaacta agtcaaatac ggtggctgaa ttacttaagt atgggtaagt
    18601 ggttaatgat ttatcgtaac ttaaataccc agtattggct aaaatagcat actgttgtgc
    18661 atattttgct tttttgcctt gtgtgtatgt gccgtttagg tgtatcgccc agtcattgtt
    18721 cacagctcga taacctatgc cgaacaatgc ttttaacggt tgaaccgtga gtaagtcggt
    18781 accatcatta cgtttgccac gagcataacc aaatccacca ttaagctcaa agccttgtgg
    18841 aattgcattc cacgctgtgt ttaagtctaa agttccgtta atatcaacac ctttgatttt
    18901 agctcggtta acattttgcg attggtaaat attgttacca atagtggcaa gatcaatcag
    18961 atttctatag tgggtatggt aagcggctac gctcaagcta cctaatgaac cattacctac
    19021 cagtgaaatt tcattaccta agcttgtttc cggttttaag ttagtatttg ctaagaaagt
    19081 ttggcttgct ccacgccctt gaacttcacg gttaaagaac atttcacttg cggtaggtgt
    19141 tctaaaacca cggctgagtt tatagccaat ttgccataca ggagagagct gataatttaa
    19201 accaatatct cccgcccaat tgttaaaggt tttatctgac aaactattat catcagaatg
    19261 taatttcgac tgctcatatc gagcatttaa atgagcagat agattttgtg tcagtaaaat
    19321 agagtctccc atcaaaatat ggaaagactt ggtttttgcc ggagactgca tattttgatc
    19381 ggttggcgta atacctgctg tataggtttg catatcaaaa ctgaaatcag atacaccata
    19441 gcctaattcc gttgacaatt tatgttcggt attacctaga tcaacaggct tgaatgtaac
    19501 acctaagcta ccttcaacat ttttagtatt aaaagcccgt ttggtattat ctgtttttgt
    19561 accacgggca atagtgattt gatcgttaat acctgaaatt tcagtatcct gtttggttac
    19621 cgcaacttta atattttcaa gaatactgtt ttttggagtg tataaataag taacgccata
    19681 agtttcaatt tcctgcacat ccaaaaaatc acgtaatgtg acggtagaat aagaaagctc
    19741 ttgaccggcg tttttactgc gttgcttaga agtttttaat tcaaggcgat tttcatcatt
    19801 ccattttagc cctaattttg ccaagtagct gtattgatta tttttgtaag gatttggtaa
    19861 accacgacca cttcccataa tatccggacc tttagacgtt gtttgtgttt cgtgtccttg
    19921 acggtggcta tacataacca aagcatccgc atattgacca tccgcagcaa tacctgccgt
    19981 ctcaatattc tctttatttt gaccgttata ggtatatttc aacatcccac caaattggcg
    20041 acctttcatt aaaatatcac ttacggtttt agttttatag ttcacagagc cgcctaatgc
    20101 cccattgcct acttttgtgc ttgagccttt ttcaatagtt acatcgctca ttaattcagt
    20161 atcgatttca gggcgagatt tattgtagta actatagcga ctaaaggtgg catagttatt
    20221 tgatgctgct tgcgatatgc catcaactgt tactgccact tgattacctt caacaccacg
    20281 gatattaaac ccttgtgggc caaagcggcc tgtttgcgga acgcccatat cagtactata
    20341 acgtactaaa tcttcattgc tcatcaccgc ttgctgttta agtaggttct cctgctgctc
    20401 atttacttca atagtatcta gttcaatgct actaatcttt gtttcttgag ctaaggagag
    20461 ttgagaaata aagggagtaa tcgctattgc taatacagtt tgctttattt gcatatttta
    20521 gatcctctag ttagtttaat ccgtttaatg ttaaaaaaat gttaacacta tatcatttta
    20581 attgaaaatt gaaataataa ttattattat ttaagtttag ataaaaatca aatggagtat
    20641 aaaatgagaa ccttactttt ttctttcatt gccctatcaa tgtgtttaaa taccacaaag
    20701 gcggcagaaa aaattgtttc aactgcgggc tatgcttccg aaattgtcgc agcactagga
    20761 aaagctgata agttagtcgg tgtggatacc acttcagtta aacctcaaac gataatggag
    20821 aaaaaaccta aaatcggtta tcgtcgccag ctttctgctg aaggtattct ctctttaacc
    20881 ccagatttga ttattttagc ccctgatgcc ggccctaacg cagttattga gcaaattaaa
    20941 gccagcggta ttccattact tcacttagga gagaaaccga gcttagcagg gattcgtgag
    21001 gatattcagc aaattgccaa ggcgattggt gcagaatcag aggcaaaaca attaacagaa
    21061 accattctgg cagatgaaaa aaaattagcc gacattcgta gccaacattc tactgacaaa
    21121 gaagtcttag tgctgattga cactgcatcg caaggtatct ttgggctagg gaaaaagagt
    21181 gtgggtgatc atttcttaac cttgttaaat ctaaacaata gtttcaatgc tgagggcaat
    21241 aagccactcg ccaatgaggc tttagctgca agcaatgccg atgttatttt attagcctcc
    21301 cgtaatcagg caaaagaatc tgcaacgatc agtaaattag cagaatccag cccacaatac
    21361 gcccaactca caaatacaca agcgggcaaa aaaggctgtg tttttgcaat caatatttta
    21421 gatgccttgg gatttgggcc ttacactgct caatatgcac accaaattct caccactatt
    21481 caaccttgtt taaaataacg gatacgcttt gaaactgcat ttaaccttaa ttttcacttt
    21541 aattatcagc ttagtggttt gtacccattt aggggcgatg tcattacccc tatccgcttg
    21601 gcactgcaac aacgctgaat gcgaaagtgc agcctatatt ttatggcaaa tccgcctgcc
    21661 tcgattacta gggacattat tcatcggggc agctctcgct attagcggag caacaatgca
    21721 agggctgttt cgcaatccgt tagtagatcc ggggattatt ggtgtctctt caggtgctgc
    21781 attggctgcg gcattatata tcgtggtgat tcagccacta ctgccggaat caatcagcta
    21841 ttatgctcta cctattgccg cctttttagg gggctggctg gtgactttta tgctctattt
    21901 actttctcaa cgtaacgggc aactacatat cgccattatg ctattaattg gtattgctct
    21961 agctgcattt agtggcgctt taaccggcat actgatttat attgccgatg ataaccaact
    22021 tcgtagttta accttttgga gtatgggatc tttagcaggt atgaattggc aaatggtggc
    22081 aatgatggct gcggttgtct tcatttgtag cccacttatt tggcgggaac accgtgcgtt
    22141 aaacgcctta acattaggtg aagaagatgc acaatacatt ggttttgaca ttgcaaaagt
    22201 caaacgccgt cttgtgtttt acgttgcctt attaaccggc acaagcgttg cctgtgttgg
    22261 ggctatcggc tttgtcgggc tggttattcc acatttaatg cgtttactgg gaggcagtaa
    22321 ccaccgccat ttattactca atagtttagt ttgcggtgca atcttattaa tgattgcaga
    22381 tactcttgcc agaaccctag ccgccccggc agaaatcccg attggtattt ttaccgcctt
    22441 atttggtgcg ccattccttg cagtaatggt atggaaaagt gggaaacata tctaatgagc
    22501 aatttattaa cttgccaaaa tttaagcatc gttcatcaac aaaaaaagtt gctggagatt
    22561 caagaaattg cctttgaaca aggaaaattt accgccatca tcggtccaaa cggagccggc
    22621 aaaacaacct tgctcaaagc cttaagcggt aataatgcac aaactaacag tgccgtattt
    22681 tatcaacatc aacgcttaac agacttaaac ggatatgctc tggctcaaaa acgggcaatg
    22741 ctcagccaac agagccaaat tgcctttccc ttaaaagtag aagaattggt acaactcgga
    22801 agagagccat atcgccaaag tgaatttgca aaatttaatg aaaaaataac cgcttgggct
    22861 atggaaacaa tgtctttaac cgcattaaaa gaaagaagta gcatacaact ttccggtgga
    22921 gaacaacatc gagcgcatat tgctcgagtg ctggctcagc tcgttcctga accgaatgcc
    22981 gatttaagcg gaagctggct tttgcttgat gaacccacca accatttgga tattcatcac
    23041 caatatcagc ttttttcctt actacaaaag ctgaaacaac aaggcttaac tatcattgcg
    23101 attttgcacg atcctgcatt ggcaataaat caagccgatc atatcttaat gctaaaaaat
    23161 ggagaaaaat ttgctgaaat gtccgctaaa gccattgcag aaagttgttt attagatgag
    23221 ctgtatgaaa tgaaaatggg ctgtcgctat tgccctcaca gcgggcaata ttatctttgc
    23281 cctgcattag ctgaaagtta gggtgcgatg caccctatcg tttaatcttc tgcatatcca
    23341 caagcaggca gtagtttacc gtctaaatac gctttgccgt gctgagaaac taatctaccc
    23401 gaacagaacc actcaatcac caacggataa tatcgatgtt cttgctcaac cactcgagcc
    23461 attacatctt caatctcatc ttcaggataa atcggcactt tagcttgtaa taccaccgca
    23521 ccagcatcca cttcttcatt cacgaaatga atagtcgtgc cgtgttcact ttccccagcc
    23581 tctatcgctc tttgataagt gtttagccct gggtatttag gtaataagga agggtgaata
    23641 ttgagtattt tgccagcaaa atgttgcgta aattcaggcg ttaaaatttt catatagccg
    23701 gctaatacaa ttaactcagc tcctaactgc tcaatttgtt cggcaatcgc aagatccatt
    23761 tccacattgc tttggtaatc tttacgagaa aatacaaatg tcgggattcc cgcctgttta
    23821 gctcgaatca gcccataggc atctgcctta ttgcaaatta caccgcaaat ttgaccgctt
    23881 gtatccgcag atttttgcgc atcaatcatt gcttgtaggt tagaaccatt ccctgaaatt
    23941 aaaaccacaa attttttcat attacacacc ggctgaatat tcatcgttct ggatattatg
    24001 ccttgaatct caacatcagt acaggaaaga aaaagggctt attggttaaa ataagccact
    24061 ttcaagctag aaacgcacct ctgcccctag tgtgtaagtt cgacctctgg cactatttgt
    24121 gaaacgatag gtgtctgtat catagtcata ctcttctgcg gtggaagagt tatacgcatt
    24181 taaggcatca agataatttc ggttcatcac attttgtacc ccggctttta aggttaaatg
    24241 tttattcact ttgtaatttg catataaatc gataatcacc ggaatattcg gtaactgctc
    24301 gtgaataatg ttgccatctt cgtctaaatc aataccgaca cgtttcgctt tgcctgtata
    24361 tttaaaaatg ctagacagtg ttaatttttt atccagcaat ttagccccta gctccaaagt
    24421 cgcataatct tttggtagct gggtataagc ttcagtgcca aatccataaa gaatagtcgc
    24481 accttttgaa gtgggtgctg tgctttcagt tcgggtataa ctaccattga caaaaaagct
    24541 atcggaatcg taagccagct caacttccca acctcgattt ttaacctttt cagggtgatt
    24601 cacataaatt tggaactgta tggcattggt agcatcactt gaatttcgac atactgttgg
    24661 cccacaacca tacaacgaga ggttatacaa atagtcttta atgcgtgagt taaaatatac
    24721 cgcttttagc cccaagatat cattattgag taatacatta tcttgatgaa tattaaaccc
    24781 aatctcatag gttttcgcac tttctggttt caaaaacggg ttcacactca agccatttcg
    24841 actggtatta aacatttctt gcacattagg cgaacgggag gtttgtttgt aactgacaaa
    24901 aggttgaaac caatcagcaa cctgaaatgc taaagtggca gaaggattca acttataatg
    24961 ttttttatta acatcaaacc caaaagaagg gaaacaataa tccatatcat cgctatcgca
    25021 ttctggctta tatcctttta actgatagcg tgaatagtga agacttgaat ttaaagtgac
    25081 aatgccgtat ttccattcgg tgtctaggta aagggaatca atattttgct tacctgccgg
    25141 cccaaaagcg gcattgctaa tcgctccatc attattctta ctttccggta aatcacggga
    25201 atatttattt cgcattaaat ttataccata agtcaataat gctgataatt tccctgactc
    25261 aaaacggctg gtgttattca aatcaaacat ccacattttg tttttggttg aggcatcttc
    25321 taatgtccat agttttgcat catcgtggaa atcttgctta gtttctgtta ctgctgttag
    25381 gaatttcaga tcaatccaat cagattgagg ctggtaactg gattctaagg aataggtcac
    25441 actatcaata ttacgcccca ctaagctatt tttatattga cgtccggaca atttaaactc
    25501 tgaatcttga tttggcttaa aggccatttt tcccatataa gattcagggt gattttttaa
    25561 cccttgcata taagcagaat ccgttgccat tgtgccatta cctcggcgat aattttgact
    25621 gatactactt ttgctaaaag ccaataaact gccaagagaa ccactttcca gcacatcaac
    25681 ttttccggct agtgcaagca ttccgttttt tcacagatca ttattgccat aattaaaacg
    25741 gcttaatacg ccgactttat gttgttctag tagaatatct tcgacatcaa tcgttttaaa
    25801 attagcacta ccggctaaag cattcaatcc ggctgcacca ttgttgtagc ctttcgcaat
    25861 atcaactcgg gttaaaaaat tttgatcaat taaagtgctg aattggctca ttggcccgtt
    25921 accatcagtg tggaacttac caccgccgtt tgctgatgta ccaagctgtg tttgtggcac
    25981 gccatcaact aaggtattta cccgaccaag accactcata ttacgaatat tcacactcac
    26041 gcccccttgc cccgggtcaa tttgggtata gccacctgcg acagaacgga caatactatc
    26101 taaactttgc attgcattca cattcattgc tcgagaactc accgagccgg ctttactgaa
    26161 attctcaaaa ttagggtttt ccacagtgcc ataaaccgtc acagtatcaa gctccgcaat
    26221 atttttcgcc ttttcctctg ccaatgcagt ttgcgatcct cccatacaca aaaccggaaa
    26281 caaaatgcgt gtgattatat taaatttcat aattctcctt attgagtgac ccaaagataa
    26341 tctgcctgtg aagaagtaat ttcttctctc tctgttttaa gcattagcac cgctactttt
    26401 tttgttcggt catattccgc aatatgcaac ggaacgccaa gatctttgcg aacgtggtgg
    26461 ttacctgcaa tcagcaagct agtcgataac gcatttttta ctaatttttc tgccattctg
    26521 cgatcacgga attgttgtac tgccaccata ttttccatcg ggtaaggcat tccgtgattc
    26581 attgacagca acagttgtgc gatcttttgc ttaacttcag aggaagtgga tacagaacct
    26641 tttaatggct ccgccccatt taataaaatt tcaacctctt tatctgttaa gttagtggct
    26701 ataacacgac tatttgaacc taagctctct accactaaat cttgatacat cttccaatcc
    26761 catttttgcc attgaattgc ctctcttaaa tccgtggctg ataaattaac cggtcggtta
    26821 aactgaatgc gatcaatcgc aggctgctga tccacattta acatctccaa cgcaattaaa
    26881 tgtaacttgg ctttcttcgt aagttgatta aataaataca attcaatttg gtggtgaatt
    26941 aactgagtat gctcctcacc aactaaaacg taatcttgag aagatagacg ggaaagtagt
    27001 tcagtaaatg aaatcgtttg attggtattc agatcaataa tttggccgat agagtccacg
    27061 ttttgttcat taagggattg cgttgaaacc gagcagccta aaagaaaaaa agccataaaa
    27121 aggctagcta aaaaagagga aatacgaggt aacataacaa aaaataaatt aatgagaatt
    27181 attatcatta tgttatgtaa aaaaagaaaa atcaatccat tttcagaaaa cagataaaga
    27241 aaatgattga taaaacaaaa agcggtaata tttctaaaaa atatttcaaa cttaagtgag
    27301 cataagg
//
LOCUS       DANJWC010000013        26339 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.013, whole genome shotgun sequence.
ACCESSION   DANJWC010000013 DANJWC010000000
VERSION     DANJWC010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 26339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26339
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.013"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..2173
                     /locus_tag="PWX02_000392"
     CDS             <1..2173
                     /locus_tag="PWX02_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HDL1728441.1"
                     /translation="APLYYSRIPAMQVTEDNKLVVMFDLRWNRAYDQDRIDPGIAISS
                     DGGHTWTKKTAWSFDRTNHPARRSMDPTLLHNPIDNSLYVMHGTWSMSDQQWYGGRVP
                     HFNSGSWAATIYKSTDGGLNWEKNTEFSKFSNLDVFSKVTKNGKPTLGFLGGVGSGIV
                     MQDGTLVFPIQTAHQNGIATTIMYSKDNGKTWEMPEIDNPVAPNQSSLENMVFELEPG
                     KLVMTGRGNSRWAYSSTDMGKTWELFTPTNGLLPTSSQPTQGSSIYVTLPNGRKVLLI
                     SKPDGKNDGWKRGDITLWMLDAKDPSHKHKVHIIRPGSGNAAGAGYSSLAYKEGNLFV
                     AFENDGDISVKNLTEHIAEMEAKAIEWGLPDELTPALDKINALPYLNKAQKETLVEKM
                     RRANDTAIAQSFVINKEMYNLKNNSDELDKLAKNITKALPSQQNIYQRALAYVNKVTN
                     TADTTYLNYNGIMDTYANLYESYLALNTKLDFTRYIQKARKFNEYNTDILYRSFDNFF
                     AHYDTGVKHNNLSLGLNTKLSENLRGGLFFEYSNKNRNSVQIGARAKYEMDNHQLSGF
                     LRYRGVKHKDMLARNNSVDGYLNYAYRIQLDDKLSISPSVGAYVSRSSRSLIDEDLAI
                     NSRTVYASDVGLNIHYKLNDIDAYIRPSIGFVNGDITLSQSNDMTNTYKIKDKSNVYS
                     VTTGLKKRFANGVALGADFKLHRYGSQTTESNFGLNVSYNW"
     gene            complement(2340..2876)
                     /gene="hpt"
                     /locus_tag="PWX02_000393"
     CDS             complement(2340..2876)
                     /gene="hpt"
                     /locus_tag="PWX02_000393"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDL1728442.1"
                     /translation="MEKHHIETLHSKEEVLNRINELAKEINQHYHQKQCQNLVVVGLL
                     RGSFMFMADLVRKLELPVEVDFLTASSYGSSTESSRDVKILKDLDGEIQGKDVLIVED
                     IIDTGFTLSKVGEILKLREPNSLAICTLLDKPCRREVDVPVKWVGFEIPDEFVVGYGI
                     DYAQKYRHLDYIAKVVIE"
     gene            complement(2915..4264)
                     /gene="pmbA"
                     /locus_tag="PWX02_000394"
     CDS             complement(2915..4264)
                     /gene="pmbA"
                     /locus_tag="PWX02_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HDL1728443.1"
                     /translation="MSITDKQTLLQQEQELRQAVEFALNFAKQAGAEAEVGVTKVAGL
                     SVSSRLEQVENIEFNNDGSLGISVYLVKRKGNASTSDLQPESIQKTVEAALAIAKYTS
                     EDACTGLADKELLAFEAEDLELYHQADISVDQAVELAIEAEHYALNADEKIVNSDGAT
                     FNSHSGVRVYGNTHGMLQSYLSSRYSLSCSVISAYEDQLERDYEYTISREFDKLQAPK
                     WVGEQAAIKAVDRLNPQRIKTGEMPVIFYNDVATGLVGHLAGAISGGALYRKSSFLLD
                     KLGEQILPSWFAISERPHLLRQLASSAFDSEGVITQDREIITDGILQTYLMTSYSGRK
                     MGLKSTGHAGGIHNWLVKPNRTGGLNSLLKEMGTGLLVTEMLGSTINSVTGEYSRGAA
                     GFWVENGEIQYPVAEITIAGQLQDMYKNLVAVGDDIEHRSNIQTGSILLEKMMVSGE"
     gene            4363..4893
                     /locus_tag="PWX02_000395"
     CDS             4363..4893
                     /locus_tag="PWX02_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HDL1728444.1"
                     /translation="MAKKRSKNEIDWTDEEEEIIWVSKNEIKRDSEHLKKIGAELIEL
                     TPQNLEKIPLDDDLKEAIRQAQGFKMEARRRQIQFIGKLLRNRDQEPIQEALDKVKNR
                     HNQQQALLHKLELVRNQLIAMGDASLDNLLTEYPQLDRQHLRNLIRGAIKEREANKPA
                     KNYREIYQYLKTEIVE"
     gene            4899..5270
                     /locus_tag="PWX02_000396"
     CDS             4899..5270
                     /locus_tag="PWX02_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HDL1728445.1"
                     /translation="MEYLSTILKAHIGLAYISLILLLTRGFLSSKMVDWRQYKILRIA
                     PHAVDTFLLITGIAAVAIFLAYDFFTLAQFSWLLPKLLFLVLYIVFATKAFKKGQPFS
                     LKFYLLAVVSFLLMMLTATFH"
     gene            5283..5996
                     /locus_tag="PWX02_000397"
     CDS             5283..5996
                     /locus_tag="PWX02_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDL1728446.1"
                     /translation="MIGPFVNAGAIILGGVLGACLGSHLPERIRTTLSLVFGLCCFCL
                     GMMLTASVKYMSAVALALILGTIIGELIYLENRIGKVADLARGLIDRLFPPVHSLSQE
                     EFLEKFIALLVLFCVSGTGIFGAIKEGMTGDPSILYIKSILDFFTAMIFAASLGYAIA
                     AIAVPLILVQGILILLATEIMPLTTANMMADFSATGGLLLFATGFRICGIKNFPVANM
                     LPALLLAMPMSYFWEWLVH"
     gene            5999..6562
                     /locus_tag="PWX02_000398"
     CDS             5999..6562
                     /locus_tag="PWX02_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDL1728447.1"
                     /translation="MKKRIIVGISGASGFQYGYKVLELLKPFSEIETHLVMTKGAELT
                     RTLETDYTREALHELADEIHSLNNVGASIASGSFKTAGMIVAPCSMRTLAAIANGFSD
                     NLLTRAADVVLKERRKLVLMVREAPLNLAHIDNMRKVTEMGGIIFPPIPAFYQQPKNL
                     DEMVTHSVSHALSLLDIDIPNTPHWGE"
     gene            6625..7065
                     /locus_tag="PWX02_000399"
     CDS             6625..7065
                     /locus_tag="PWX02_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDL1728448.1"
                     /translation="MHNPIPTHILDFIQTNHVVNIAAHTEQDFWAANCFYAFDQENAR
                     LIILTAKKTRHAGLMLENPHIVGTICGQIEKINEIEGVQFSATAECLEDKQAEKAALQ
                     IYYQKHPLARLKPSDVWVLSFNTIKHTGNKLMFAKKTIWERNII"
     gene            complement(7069..7944)
                     /locus_tag="PWX02_000400"
     CDS             complement(7069..7944)
                     /locus_tag="PWX02_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1728449.1"
                     /translation="MMKTILIDEANQMKGFSHLYRLRSDLMLKYTEISVTETVEQEPA
                     EFEAGLRLVILLSGRSEIDFNSEVFELDASLKPQAAFLPLNRAEMGRKIFRKGQLQKE
                     LVIFLYPKWLIESQLNLASYHHFKQHLKAHQIILTQSMLKLIHDIVSEQEMQPFGLLE
                     KEGNLLALLASAFSQLTQAPRLKKDRVQQLTELLESGKFDGWSLKQIAIYFHTNVTTL
                     QNEFQHYHKTSIFAYLRRSKLEQAYKLLLQGQSITQAAEFAGYANSENFSTAFKRTFG
                     ITPSQIKRDKAKGII"
     gene            8050..10125
                     /locus_tag="PWX02_000401"
     CDS             8050..10125
                     /locus_tag="PWX02_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HDL1728450.1"
                     /translation="MKIKKYSLSLITLAIVAPQVLAETTSQAELNAIEVKEKLAATTG
                     YIPVYTTVGTKTLTSLARTPFSINVVSDDLMHDQAVRSVTDSVAYTSGMMGGYRGNNS
                     LIEISIRGIGNKSDGSTIPTYLNGSRYQTAFEIDPFFLESINVIKGPNSILYGQANPG
                     GVMDIITKKAGSNPHRHLQFVVGNHQRYQIGLDIEENLSNTLSARLIGQLERTNWNTQ
                     FVKEKGGAFAPSILWKPTTNTELNVYAYYAKKPKAGDRNFLPKEGTINSVNGKKLPYD
                     MFLSDLNYHGLKAEQMQVGYNFSHKFTPNLTFRQNASYHNGEENFKNLVYWGDVGSVL
                     ERRARRWDVKTQEINLDNQLEWVFSTANINHTLLAGIDYKRVREDLDNYMGIAPDFDW
                     QNPVYGVTVTEPSLFSSEVKHLKQLGVYLQDQIEIGNFDFLLGGRYDRAKNSNQDRLN
                     SKTENNQEGKFTWRTGVVYNFENGIAPYISYSTSFVPSAEKDAQGNFLKPTTAGQWEV
                     GMKYQPTQDTLLTLAGFKITQKDLANYQWQTRSYEQIGEVQTKGIEVSLNQQLSDKFS
                     IAPSYAYLKKEITADKDGTTIGKTQWGVPRHQASLWAKYKFFDKLTAGVGVRYMGTTF
                     GDNQNTFTVPAYTLYDMMLAYDFMPNLNLQLNAQNLTNKKYVASCANNFSCFYGKDRN
                     LSATLNYHF"
     gene            10169..10390
                     /locus_tag="PWX02_000402"
     CDS             10169..10390
                     /locus_tag="PWX02_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acid transporter"
                     /protein_id="HDL1728451.1"
                     /translation="MKKTTACILTFLVSSVLYANNITIELKQNDFVQSKLIQKAQQPL
                     SLSIIFPAKALACWLKMSLVNKILCLNQN"
     gene            10372..11736
                     /locus_tag="PWX02_000403"
     CDS             10372..11736
                     /locus_tag="PWX02_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3327 domain-containing protein"
                     /protein_id="HDL1728452.1"
                     /translation="MFKSELSGTATFSILANGATVSDQDFTLTIERHIPISAQIAPPK
                     VIENTQLEELSQKIAKNPEKQTALINDFWDKAKQAGTPLIEPLDEQQSRVTFLWRGAT
                     KNVRIWGGVSTEHDFMQRFGETDIWYKSYIVPNDTLVEYKLAPDVPTLPVDENTQRRA
                     LLATAQADPLNKTAYFEKIGQNIHNTDKFNYASLLKLPNAPTQSFLTEAPNIAKGKLQ
                     QLIFESATLGNKRRLFVYLPDGFSAEKNYAVLYLFDGVEYTERVPTKTILDNMIAQKV
                     ILPTVAVFIENPSSESRRVELPANPIFTQVLAKELVPFVEKQLNIKTKERIIGGSSYG
                     GLSSAYTVLNYPNVFSKALILSGSFWWQPKGTPSEQSHYIAQQFIQKEKLPLCFFISA
                     GFYESGKSAILETSRHLKDVLLAKGYPVTYVEQSTSHGYLAWQGLISEGLKTLLGSNK
                     CTSN"
     gene            complement(11733..11945)
                     /locus_tag="PWX02_000404"
     CDS             complement(11733..11945)
                     /locus_tag="PWX02_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDL1728453.1"
                     /translation="MNYSLDLTDYACPLPLLMAKKAMNDLKKGDSLEILLNQQSSLTD
                     FELLAKEQGWLCELQAVENRVIFTIA"
     gene            complement(11993..12504)
                     /locus_tag="PWX02_000405"
                     /pseudo
     CDS             complement(11993..12504)
                     /locus_tag="PWX02_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357669.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
     gene            complement(12517..13671)
                     /gene="tgt"
                     /locus_tag="PWX02_000406"
     CDS             complement(12517..13671)
                     /gene="tgt"
                     /locus_tag="PWX02_000406"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDL1728454.1"
                     /translation="MKYELKTTSGNARRGRLTFNRPKGEFHVETPAFMPVGTYGTVKG
                     MTPEEVKATGAQILLGNTFHLWLRPGQEVMKMHGDLHDFMQWHGPILTDSGGFQVFSL
                     GKLRKIKEEGVTFQNPISGEKIFLSPEKSMEIQYDLGSDIVMIFDECTPYPATFDYAK
                     KSMEMSLRWAKRSRDRFDELENPNALFGIIQGGIYEELRKVSVEKLVEIGFDGYAVGG
                     LAVGEPKEDMHRILEYVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNA
                     RNGHLFVTNGIVKIRNAKYRNDITPLDPECDCYTCKNYTKAYLYHLDKCGEILGARLN
                     TIHNLRYYQRLMAEIRQAIEEDRFDDFVVEFYARIGKEVPPLQSEVNGQK"
     gene            complement(13730..13870)
                     /locus_tag="PWX02_000407"
     CDS             complement(13730..13870)
                     /locus_tag="PWX02_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728455.1"
                     /translation="MKKLLLCVGILVLAACSSTGVGGSIGAGGGSNGVGIGLGVGTDF
                     RF"
     gene            13964..14536
                     /locus_tag="PWX02_000408"
     CDS             13964..14536
                     /locus_tag="PWX02_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1439 domain-containing protein"
                     /protein_id="HDL1728456.1"
                     /translation="MKILRKFAAVCVVSMAMLSANGNLLSISEQEINDYLATKLAEKI
                     PLQDKVGVPHLFELDYHLHSLTSQIGRTEEKKVAISGVVDGLLKAKGKKYDASIQLNM
                     DTTPYYDPQKGALFLKDVRLLSWSATPAKYQAELQMFLPVLADGLNSFLNSTPVYTLD
                     ENKAKEAMVKKFGKAIVIENGNIKLETTIF"
     gene            14638..14856
                     /gene="cspD"
                     /locus_tag="PWX02_000409"
     CDS             14638..14856
                     /gene="cspD"
                     /locus_tag="PWX02_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein CspD"
                     /protein_id="HDL1728457.1"
                     /translation="MDIGIVKWFNNVKGFGFITSDVCEGDIFAHFSEIQAEGYRSLKV
                     GQKVQFELVQGERGASASNITPISEKTL"
     gene            14947..15861
                     /locus_tag="PWX02_000410"
     CDS             14947..15861
                     /locus_tag="PWX02_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL1728458.1"
                     /translation="MLSPYQLVLINLLYIFGIGLNIVLLRFLSLHFDPLNNNGVRFLI
                     GGLTLLVFIFWRFRTSLSQLIKTPKLIAISLFVGLMMSANMYFWLKGVKITNAVTASI
                     FGVLAMPFGVIIAAIFFQDERERIKSKAFWFGSILTIIGSLGFIWHGKTLSIGEGFIL
                     GSMFLFLSIIIRNIQNLVVKFARELNVFTLSCVTSLTTSLSSLLLSQQAGKFNELFTA
                     PNWLLFSLMAAGIYAIMIGMVLTFHIIQTQGLITYQVLELLMPVSTAIIAYILLDERI
                     SIVQMGFACIVILGSSIALKLVKYPKHY"
     gene            15976..16836
                     /gene="araC"
                     /locus_tag="PWX02_000411"
                     /pseudo
     CDS             15976..16836
                     /gene="araC"
                     /locus_tag="PWX02_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250927.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
     gene            17023..17997
                     /locus_tag="PWX02_000412"
     CDS             17023..17997
                     /locus_tag="PWX02_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL1728459.1"
                     /translation="MKKPTFKHALFSSILALACYGSAFANDVVNISKIDGMPWFNRMA
                     EGVVQAGKDNNINAYQVGPSNTDAPQQVKLIEDLIAKKVSAISIVPNDANALEPVLKK
                     AKQNGIVVLTNESVGQPSADWDIEIIDNAQFAADYVEEVAKAMGGKGGYVIYVGGLTV
                     PQHNLWADLFVKYQKEHYPDMFEVTSRMPVAENINDARRTTIDLVKAHSDLKAVVSFG
                     SQGPIGAGLAVKEKRLKNKLSVFGMMIPSQAANLIKSGDITMGITYDPANAGYALAAV
                     AAKVLKGEKIEDGLEIPTVGKAKVDSEKKLLQFHNVLKVTKENIDKLY"
     gene            18054..19544
                     /locus_tag="PWX02_000413"
     CDS             18054..19544
                     /locus_tag="PWX02_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL1728460.1"
                     /translation="MAFISMRHISKTFHGIKALNQVNLSLDYGEALCLAGQNGCGKST
                     LIKILSGVYQPDKGAEIQIGATQYTKLTPQESIDKGIQVIYQDLALFPNLTVAENIAI
                     NLHRKFGWVSQDHIHQVALTAIQSINANLDLNAILEDLPIAQQQLVAICRALAQNARL
                     LIMDEPTASLTAKEVKDLLDVVIKLKSKGISIIFVSHKLQEVMSVSDSVLVLKDGNMV
                     GQYPIREMNEKRLGFLMTGLEIDYKRLDLPDFLQRKTVLEVRNLTLPHQYQEINFSLR
                     EGEIVALTGLLGSGRTELCLSLFGITQPQSGDILLNGEKVIFNTNRDAIAKGIAYVSE
                     DRMHTGLIMAESIHHNIISTIFHKITDKLQIIQSSKAYASSQALIDSLKIKVSDSDLP
                     VNTLSGGNAQRVSIAKWLAIDPKIIILDAPTIGVDIANKEGIFQIIKSLAQQGIAVIF
                     VTDEVEETYYNSHRVIVMKKGRIVDEILPAYSSEKSIAEVIYENQQ"
     gene            19528..20487
                     /locus_tag="PWX02_000414"
     CDS             19528..20487
                     /locus_tag="PWX02_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1728461.1"
                     /translation="MKISNNHILSSTLLILILLLTLFTRDFATIENLYDVVNNYAMLM
                     ILACGLFVVLLSGGIDISFPAITIISQYVMVTLIGKYDVGFAGVFVISSTIGILLGFI
                     NALFVNRLSVPSIIITISTLNIFYGGLLYFTKGVWLYEYPEWFTQEIVFFKQITAEGV
                     EFGLSFQALMAILAVILTGLITNKLTIGRKIYALGGNPDATSRIGFNLLFLHLFAYGY
                     MGFMAGLAGIVQSYTVQSVAPDSLLGYELTVLAAVVLGGASLSGGRGTLLGTVMGVML
                     LAIIQNGLNLMNVSSYWQTIITGIIIILSISATALSQRKKQGA"
     gene            20490..21440
                     /locus_tag="PWX02_000415"
     CDS             20490..21440
                     /locus_tag="PWX02_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1728462.1"
                     /translation="MKSKLDPTIFYLLALFAVAVIAFSIFIPDIFWSVSNFQSMASQI
                     PVLGILTLAMALPMLTGGINLSIIAGMNACSLIIAYFITQYTGISWLFIAILLSLCCA
                     LAIGLLNGTLIAIIRVSPILATLGTMTLINGINILVTNGSTIANFPETMLSINASTFL
                     WIPLPILIFLFLAGLIWFFLEKTAMGKAVYFIGNNEKATYYSGINTKKVLIWVYVISS
                     ILCVFAAILMMSKLNSAKASYGDSYLLISILAAVLGGINPDGGKGKLLGIMLALVLLQ
                     VIESGLNMLGISSYITMILWGSLLLFFIFLQKNRLFRLTQ"
     gene            21453..23048
                     /locus_tag="PWX02_000416"
     CDS             21453..23048
                     /locus_tag="PWX02_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDL1728463.1"
                     /translation="MQNEKQLIEQGNTVIGIELGSTRIKAVLISSDGTILATGGADWE
                     NRLIDGIWTYHQNEIWEKLQAAYMDLSKTVKEKYHTTMRTAKALGISAMMHGYLPFDK
                     HGNQLVAFRTWRNNITLTSSEKLTALFQYPIPQRWSIAHLHQALLNQEPHLPEIDYLT
                     TLSGYVHWQLTDHKVLGIGDASGMFPIDTELKSYNPTMLAQFDRAIADYRMPWKLEAI
                     LPQVRVAGEQAGKLTEKGAKLLDPTDNLQAGIPLCPPEGDAGTGMIATNCIKEKTGNI
                     SAGTSAFAMIVLEKALSKAYSELDIVTTPAGKLVAMAHANNCTSDINAWVNLFGECLS
                     VFGVNICTEELYEMLFLHALQGDADCGGLLSYGFHSGEHNVGLSEGCPVFLHPTNAHF
                     NLANFMRTHLYTAFGAMKLGMDILINQEKVEISRILGHGGIFKTEGVAQKILSSALNI
                     PLATASTASNGGAWGIALLADFLSVSDRYRLEEYLDNCIFNTTKLNLVQPDKTMSEGY
                     ERFMQRYKQGIPVVEKALFLNTD"
     gene            23272..24474
                     /gene="yedE"
                     /locus_tag="PWX02_000417"
     CDS             23272..24474
                     /gene="yedE"
                     /locus_tag="PWX02_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HDL1728464.1"
                     /translation="MLAIWQSFKQNYLIKYWNPVAAVIAAGIISAYYFGITGTYWAVT
                     GEFTRWGGHLLNALGVDVSNWSYFQLIGLDGTIFTRIDGVMILGMFAGCLSAALWANN
                     VKWRNQPHKKRIIQALIGGALAGFGARLAMGCNLASLFTGIPQFSVHAWFFTFATAIG
                     TYFGVKVTLLPMFRVKLELKKGAAKIKENDPKQANRRFWVGMLIFITYLSASLYVMQS
                     SIKLGFAMLCGLAFGLLIERAQICFTSAFRDLWITGRAYMAKAIIFGIIAGTIGVFSY
                     IQLGVSPKILWAGPNAIIGGLLFGFGIVLAGGCETGWMYRSMEGQVHFMWVGVGNVVG
                     STLLAYYWDDLAPVLTLDYEKINLLKEFGPVGGLFVNYGLLILCLIAVVWWEKRFLAK
                     AKAKLATA"
     gene            24515..24784
                     /gene="yedF"
                     /locus_tag="PWX02_000418"
     CDS             24515..24784
                     /gene="yedF"
                     /locus_tag="PWX02_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007241376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDL1728465.1"
                     /translation="MAFTVVQKLDKDPKDITPDYTLDTLGEPCPYPAITMLETMPQLQ
                     KGEILELLSDCAQSINNIPIDTKNHGYTLLSVEQDGTKLRYLIQR"
     gene            24885..25229
                     /locus_tag="PWX02_000419"
     CDS             24885..25229
                     /locus_tag="PWX02_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL1728466.1"
                     /translation="MNKYALLAIFWFIIGIYALLFRESTNDIPPFPHFDKVSHFALFF
                     IQIWLSANAFIRSKKGIPYFGLIIFALLYAFTSEWAQATFTSTREGSWLDGLADMLGA
                     SLALLLVKYCKK"
     gene            complement(25292..26239)
                     /locus_tag="PWX02_000420"
     CDS             complement(25292..26239)
                     /locus_tag="PWX02_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HDL1728467.1"
                     /translation="MNRITQILNIKHPIVQAPMSWLTDAHLVASVAEAGGLGFLAPHA
                     GQPTNPTSNEEVLDRMRNEIRKVKALTDKPFGVPFILSYDFSLIPLMVDLFIEERVPV
                     VLDNGWLDPEIYAKFKAAGIKIICRLPNPNLADALKAQALGADILVLTGFDEGGTLPM
                     KEVGSFNVLAEFVGKVDLPIMLAGGIADKKAVQAALTLGAEGVWIGTAFIATEECRAS
                     EKVKNWIVEANANDLLLFRTEPHYYRSLPTELAKKCFEMSENGATRSEIAKVMNASTG
                     MRLGMLEGDFENGYVSVGNGISAIDRIKTVKELIDELMA"
BASE COUNT         7878 a         5279 c         5111 g         8071 t
ORIGIN      
        1 tgcgccatta tattatagcc gtattccagc tatgcaagtt accgaagata acaaattagt
       61 tgttatgttt gatttacgct ggaatcgtgc gtatgaccaa gatcgtattg atcctggtat
      121 tgcgatttct tccgatggtg gccatacttg gacgaagaaa accgcttggt cttttgatag
      181 aactaatcac ccagcacgcc gttcaatgga tccaacgcta ttacataatc ctattgataa
      241 tagtttatat gtaatgcacg gaacttggtc tatgtcggat caacaatggt atggtgggcg
      301 tgttcctcac tttaatagtg gcagctgggc agctaccatt tataaatcta ctgatggtgg
      361 tttaaattgg gagaaaaata ctgagtttag taaattcagc aatttagacg tattttctaa
      421 agtcaccaaa aatggaaaac ctaccttagg ctttttaggc ggggtcggat ctggaattgt
      481 aatgcaagat ggtacgttgg ttttcccaat tcaaaccgca catcaaaatg gtattgcgac
      541 cactattatg tattctaagg ataatgggaa aacttgggaa atgccagaaa ttgacaaccc
      601 agttgcaccg aaccaaagtt ctttagaaaa tatggtgttt gaactcgaac cgggtaagct
      661 agtgatgact ggacgaggaa acagtcgctg ggcatactct tctactgata tgggtaagac
      721 ctgggaatta tttactccaa ctaatggatt attaccaact agctcacaac caactcaagg
      781 ctcttctatc tatgtaacac taccaaatgg tcgaaaagtg ctactaatct ctaaaccaga
      841 tggaaagaat gatgggtgga aaaggggtga tattacactt tggatgctag atgcaaaaga
      901 tccatcgcac aaacacaagg tgcatattat tcgtccaggt tcaggtaatg ccgctggtgc
      961 tggttattcc tctttagcct ataaagaagg gaatttattt gttgcttttg aaaacgatgg
     1021 tgatatttca gtaaaaaatt taactgaaca tattgctgaa atggaagcaa aagctatcga
     1081 atggggatta ccagatgaac ttactcctgc tttggataaa atcaatgcac tgccttatct
     1141 caataaagct caaaaagaga cgctagtcga aaaaatgcgt cgtgctaatg atactgcgat
     1201 tgcacaatct tttgtgatta acaaagaaat gtataatctg aaaaacaata gtgatgagct
     1261 ggataaattg gcaaaaaata ttactaaagc actgccgtct caacagaata tttatcaaag
     1321 agccttagcc tatgtgaata aagtcaccaa tacagcggat accacttatt taaattataa
     1381 tggtattatg gatacctatg ctaatttata tgaaagctat ttggctttaa atactaaatt
     1441 agacttcact cgctacatcc aaaaagcgag aaagttcaat gaatataaca ctgatatttt
     1501 atatcgtagc tttgataatt tctttgccca ttacgacaca ggtgtaaaac acaataatct
     1561 atccttaggc ttgaatacaa aattaagtga gaatttacgt ggtggcttat tctttgaata
     1621 cagtaataaa aatcgtaata gcgtacaaat tggtgcaaga gctaaatatg aaatggataa
     1681 tcaccaactt tctggcttct tacgctatcg tggcgtaaaa cataaagata tgctagcacg
     1741 taataacagt gttgatggct acctaaatta tgcttatcgt attcaattag atgataaatt
     1801 aagcattagc ccatcagttg gtgcttacgt ttcacgttct tctcgctcat taatagatga
     1861 agatcttgcg attaatagcc gcacggtcta tgcaagtgat gtaggattga atatccatta
     1921 taagctaaat gatattgatg cttatattcg tccaagtata ggctttgtca atggtgatat
     1981 aactttatct caatctaatg atatgacaaa cacatacaaa ataaaggata aaagtaacgt
     2041 gtattctgtc acgacagggt tgaaaaaacg ctttgcaaat ggtgtggcat taggggctga
     2101 tttcaaatta catcgttatg gttctcaaac tactgaaagt aatttcggtc taaatgtaag
     2161 ttataactgg taataccgta aactgattac gatttaaaag ttagactagc ccaagactga
     2221 gttctatatt ccatagagct cagtctttta atttttcttt ctcgataaga atattttata
     2281 tttcaacctt aatcgagaat gtaattagtc gtgaaaatct aagtagtgat tgagtttttt
     2341 tattcaatca ctactttagc aatataatct aaatgacgat atttttgggc ataatcaatc
     2401 ccataaccca ccacaaattc atcggggatc tcaaatccca cccattttac gggcacatca
     2461 acttcgcggc ggcaaggttt atctaataaa gtacaaatag caagtgaatt tggctcacgt
     2521 aacttgagaa tctcccctac cttgcttaag gtgaagccag tatcaataat atcttctaca
     2581 atcaatacat ctttaccttg aatttcgcca tctaaatctt ttagaatttt gacatcacgg
     2641 ctggattccg tgctgctgcc atagcttgat gcggttagaa aatccacttc tacaggtaac
     2701 tctagctttc ttactaaatc tgccataaac ataaacgagc ctcgcaataa accaaccact
     2761 acaaggtttt ggcactgttt ttggtgatag tgttgattga tttcttttgc taattcattg
     2821 attcggttta atacttcttc ttttgaatgt agcgtttcga tatgatgttt ttccattgat
     2881 ttctccaaat aaaattgatt acaagcggcc gtttttactc gcctgaaacc atcatttttt
     2941 ctaataagat tgaacctgtt tggatatttg agcggtgttc aatatcatcg cctaccgcca
     3001 ctaaattttt atacatatct tgcagttgtc ctgcaatggt aatttctgca accgggtatt
     3061 gaatttcgcc attttcaacc cagaaacctg ccgctccacg agaatattcg ccagtcacgc
     3121 tattaattgt cgaacctaac atttcggtca ctaacaagcc cgttcccatt tccttgagta
     3181 agctgtttaa cccaccggtg cggtttggct ttaccagcca gttatggata ccgccggcgt
     3241 gtcctgtgct tttcagtccc atttttctac ctgaatagct ggtcattagg taagtttgta
     3301 agatgccatc ggtaataatt tctcggtctt gggtaattac cccctcgcta tcaaaggctg
     3361 atgaagcaag ttggcgtaat aaatgtgggc gttctgaaat cgcaaaccag cttggcaaaa
     3421 tttgctcgcc aagtttatct aacaaaaagc tggatttacg gtataacgct ccaccactaa
     3481 tagctccggc caaatgcccc actaaaccgg ttgccacatc gttatagaaa atcacaggca
     3541 tttcgccggt cttaattcgt tgcggattca atctatctac cgctttaatt gcagcttgct
     3601 cccctaccca tttcggtgct tgtaatttat caaactcacg ggaaatggtg tattcataat
     3661 cacgctcaag ctgatcttca taagctgaaa tcacgctaca agagagtgaa tatcggctcg
     3721 acaggtaact ctgcaacatt ccgtgcgtat tgccataaac acgcacgcca ctgtgagaat
     3781 tgaaagttgc cccgtcacta ttcacaattt tttcgtctgc atttaaggca taatgctccg
     3841 cttcaatcgc caattcgact gcttgatcaa ctgaaatatc cgcttggtgg tacagttcca
     3901 aatcttctgc ctcaaaggca agcaattctt tatcagcaag cccggtacaa gcatcttctg
     3961 aggtatattt tgcaatcgca agggctgctt caaccgtttt ttgaatcgac tctggctgaa
     4021 gatcggaggt ggaagcatta cctttgcgtt tgactaaata aaccgaaata ccgagagaac
     4081 catcgttatt gaactcgatg ttttcaactt gctctaaacg gcttgaaacc gaaagcccgg
     4141 caactttagt cacgcctact tccgcctctg ccccagcctg ttttgcaaaa tttaaggcaa
     4201 attcgaccgc ttgtcgtagc tcctgttctt gttgtaacaa ggtttgttta tctgtaattg
     4261 acattttgtt ccattgattg attgcattga tgaatatatt ctaactcaaa tttctgttag
     4321 aatacggcat tttttagagt ttatgtaatg aaaggataaa gaatggctaa aaaacggagc
     4381 aaaaacgaaa ttgactggac agatgaggaa gaagaaatca tctgggtcag caaaaatgag
     4441 ataaaacgag attctgaaca cttaaaaaaa attggggctg aattgatcga attgacccca
     4501 caaaatttgg aaaaaattcc actagacgat gatttaaaag aggcaattcg ccaagctcaa
     4561 ggctttaaaa tggaggctcg ccgccgtcaa attcaattta tcggcaaatt attacgcaac
     4621 cgtgaccagg agccgatcca agaagcctta gataaagtga aaaatcgcca taatcagcaa
     4681 caagccctat tacataaatt ggaattagtg cgtaatcaac tgattgcaat gggcgatgca
     4741 tcattagata atttattgac ggaatatcct caattagacc gccaacattt gcgtaattta
     4801 attcgtgggg caattaaaga gcgtgaagcc aataaaccgg caaaaaatta ccgtgaaatt
     4861 tatcaatatt taaaaactga aatcgtggag tgattattat ggagtaccta tcaaccattc
     4921 ttaaagctca tatcggctta gcttatatta gtttaatttt attattaacc cgtggatttt
     4981 tatcgtcaaa gatggtggat tggcgacaat ataaaatttt gcgcattgcc ccacacgcag
     5041 ttgatacctt cttacttatc accggtattg ctgctgtcgc tattttctta gcttatgatt
     5101 ttttcacctt agcacaattc agttggttgc taccaaagtt actcttctta gtactttata
     5161 ttgtatttgc gacaaaagcg ttcaaaaaag gacaaccgtt ttcgctcaaa ttttatctgt
     5221 tagccgtagt ctcattctta ttaatgatgc taactgccac ttttcactaa ataagggaaa
     5281 ctatgatcgg gccttttgtg aatgccggtg caattatttt agggggcgtg ctgggtgctt
     5341 gtttagggag tcacctgcct gaacgtattc gcactacgtt gtcgctggta ttcggcttat
     5401 gctgtttctg cttaggtatg atgctgactg cctcagtaaa atatatgtct gcggttgcct
     5461 tagcattgat tttaggcacg atcatcggtg aactgattta tttagaaaac agaatcggta
     5521 aagttgccga tttagctcgt ggcttaattg accgcctatt cccaccggtg catagtcttt
     5581 cccaagaaga gtttttagag aaatttattg ctctgcttgt gctgttttgt gtgagcggta
     5641 ccggcatttt tggtgcaata aaagagggaa tgacgggtga tccgagcatt ctttacatta
     5701 aatcgatttt agacttcttc actgcaatga ttttcgccgc ttcattaggt tatgccattg
     5761 cagcgatcgc agtgccgtta attcttgtgc aaggtatctt aattctacta gcaacggaaa
     5821 taatgccttt aaccactgcc aatatgatgg cagatttctc tgcaacgggc ggcttattgt
     5881 tatttgcaac cggttttcgc atttgtggga ttaaaaactt cccagtggcg aatatgctac
     5941 cggcactgct ccttgcgatg ccaatgtctt atttctggga atggttggta cattaatgat
     6001 gaaaaaacgg attattgtcg gcattagtgg ggcaagcggt tttcaatatg gctataaagt
     6061 gttggaactg ctgaaaccgt tttcagaaat tgaaacccac cttgtaatga ccaaaggggc
     6121 ggaactgact cgcacgcttg aaacagatta tacccgagaa gctctacacg agttagctga
     6181 tgaaatacat tcgctgaaca atgttggtgc aagcattgcc agcggttcat ttaaaaccgc
     6241 aggaatgatt gttgcccctt gctctatgcg aacgcttgcc gctattgcta atggatttag
     6301 cgacaattta ctaacccgtg cggcagacgt ggtgttgaaa gaacggcgta aactggtatt
     6361 aatggtgaga gaagctccgc tcaaccttgc tcacattgac aatatgcgaa aagtgaccga
     6421 aatgggcgga attatttttc caccgattcc ggcattttat caacaaccta agaaccttga
     6481 tgaaatggtc acccacagcg tgagccacgc cctttccctg ttggatattg acattccaaa
     6541 taccccacat tggggagaat aattcggctt atcttttgct ttttgaaatg ctagaatacc
     6601 tgataaaaac tatcaggtat ttttatgcac aatccaatcc cgacccatat tttagatttc
     6661 attcaaacta accacgttgt caatattgca gctcataccg aacaggattt ttgggcagca
     6721 aattgctttt atgcctttga ccaagaaaat gctcgcttga ttatcttaac cgccaagaaa
     6781 accagacacg ctggcttgat gcttgaaaac ccacatattg tcggcacaat ttgtgggcaa
     6841 attgagaaga tcaacgaaat tgaaggtgtc caattctccg ctaccgccga atgtttagaa
     6901 gataaacaag ccgaaaaagc cgctttgcaa atttattacc aaaaacaccc gcttgcacgg
     6961 cttaagccta gcgatgtttg ggtattatca ttcaatacca tcaagcacac cggtaataaa
     7021 cttatgtttg cgaaaaagac gatttgggag agaaatataa tttgaagctc aaataatgcc
     7081 tttagcctta tcccgtttga tttggcttgg tgttatccca aaagttcgct taaatgccgt
     7141 tgaaaaattc tccgaattgg catatccggc aaattcagct gcttgtgtaa tactctgccc
     7201 ttgtaatagt aatttatagg cttgttctaa tttagaacgc cttaaatacg caaaaataga
     7261 ggttttgtgg taatgctgaa attcattttg taatgtggtc acattggtat ggaaatatat
     7321 tgcaatctgc ttcaaactcc agccgtcaaa tttaccgctc tcaagcaatt ctgttaactg
     7381 ttgcacccga tcttttttta accggggagc ctgtgtcagc tgagaaaaag cacttgctaa
     7441 taaagctaat agattacctt ctttctcaag taacccaaat ggttgcattt cctgctctga
     7501 cacaatatcg tgaataagtt ttaacatact ctgagtcaaa ataatttggt gggcttttaa
     7561 atgctgttta aagtgatgat agcttgccaa attcaactgg ctttctatta gccatttagg
     7621 gtagagaaag ataacaagct ctttttgaag ttgccctttc ctaaaaattt tacgccccat
     7681 ttcagctcta tttagcggta aaaatgcggc ttgaggtttt aaacttgcat ctaattcaaa
     7741 tacttccgaa ttaaaatcaa tttccgatcg accactcaac aatataacca atcttagtcc
     7801 ggcttcaaat tccgcaggtt cttgctctac ggtttcagta actgaaatct ctgtatattt
     7861 cagcataaga tctgaacgta gtcggtataa atgtgaaaaa cctttcattt ggttagcttc
     7921 atcaatcaag attgttttca tcattaaaaa ctttttttcg catagtaaaa attttatttt
     7981 acaaaggttt tttgcataaa aacaagatat tattatattc aataatcatt cttatttaag
     8041 gataaactaa tgaaaataaa aaaatattca ctttctctaa tcactttagc gattgtagct
     8101 cctcaagtat tagcagaaac aacctctcaa gcagaactaa atgctattga ggtcaaagag
     8161 aaattggcag ctaccaccgg ctatattcct gtttatacga ctgtaggaac gaaaacatta
     8221 acctctttgg ctcgtacacc ttttagtatc aatgttgtct ctgatgattt aatgcatgac
     8281 caagctgttc gttctgttac cgattcagtg gcttatacct ccggtatgat gggtggctat
     8341 cgtggtaaca attcgcttat cgaaatctcc attcgaggca ttggcaataa aagtgatggc
     8401 agtacaattc cgacctattt aaacggttct cgctaccaaa ctgcatttga aatcgatccg
     8461 ttcttcttag aaagtatcaa tgtgattaaa ggtccaaact ccattttata cgggcaagct
     8521 aatcccggtg gcgtaatgga tattatcacc aaaaaagccg gcagtaaccc acatcgccat
     8581 ttgcaatttg tggtgggaaa ccatcaacga taccaaatcg ggctggatat tgaagaaaat
     8641 ctctccaata ccttatccgc tcgtttaatc ggtcaattag aacgaacaaa ctggaatacc
     8701 caatttgtaa aagaaaaagg cggtgctttt gctccaagca ttttatggaa accgactaca
     8761 aacacagaac ttaatgttta cgcttactat gcaaaaaaac cgaaagccgg tgatcgtaac
     8821 tttttaccga aagaaggaac gattaatagc gtaaacggga agaaactacc ctatgatatg
     8881 ttcctcagcg atctgaacta tcacggttta aaagccgaac aaatgcaagt aggttacaat
     8941 ttctcacata aatttacgcc aaacctaacg ttccgccaaa atgcaagtta ccataatggc
     9001 gaagagaatt tcaaaaatct agtttattgg ggcgatgtcg gttcggtttt agaacgtaga
     9061 gctcgccgtt gggatgtcaa aacccaagaa atcaacttag ataaccaatt agaatgggta
     9121 tttagcacag caaatataaa tcataccttg ttagccggta ttgattataa acgagtgcgt
     9181 gaagatcttg ataactatat ggggattgcc cctgattttg attggcagaa ccctgtttat
     9241 ggcgtgaccg ttactgagcc tagcttattt tcaagtgaag tgaagcattt aaaacaactt
     9301 ggggtttatc tacaagatca aatagaaatc ggtaattttg acttcctgtt aggcggtcgt
     9361 tacgatcgag caaaaaacag caaccaagat cgtttgaaca gtaaaacaga gaataaccaa
     9421 gaaggaaaat ttacttggcg tacaggcgta gtttacaact ttgaaaacgg tatcgctccg
     9481 tatatcagct acagcacttc ttttgtgcca tcggctgaaa aagatgctca aggaaatttc
     9541 ttaaaaccaa caactgctgg gcaatgggaa gtgggaatga aataccaacc tacccaagac
     9601 accttactga ccttagcagg atttaaaatt acacaaaaag atttagccaa ttatcaatgg
     9661 caaacccgct cttatgagca aattggtgaa gtgcaaacca aaggaattga agtgagttta
     9721 aatcagcaat tatccgataa attttcaata gcgccttctt atgcttactt aaaaaaagag
     9781 attactgcag ataaagatgg cacgacgatt ggaaaaacgc agtggggagt gcctcgccat
     9841 caagcatctc tttgggcaaa atataaattc tttgataagc tgaccgccgg tgtaggtgta
     9901 cgctatatgg gaacgacttt tggcgataac caaaatactt ttaccgtacc ggcatacaca
     9961 ctttacgata tgatgctagc ttatgatttt atgcctaact taaacttaca gctcaacgca
    10021 caaaacctga cgaataaaaa atatgtcgca agctgtgcca ataatttttc ctgtttctat
    10081 ggaaaagatc gcaatttaag tgcaacgtta aactatcatt tttaatctca acagccctca
    10141 aatgagggct taaacttttg caaaaaatat gaaaaaaacg accgcttgta ttcttacttt
    10201 tttggtttca tctgtgcttt atgctaacaa tataaccatt gagcttaaac agaatgattt
    10261 tgtgcaaagc aaattgattc agaaggctca acagccactc tccttgtcga ttatttttcc
    10321 tgccaaagct ctcgcctgct ggctcaaaat gtctttggtg aacaagattt tatgtttaaa
    10381 tcagaactaa gcggcacagc tactttttct attctagcga atggtgcaac cgtttcagac
    10441 caagatttta ccttaacgat tgagcggcat attccaatct cggcacaaat tgctccacct
    10501 aaagtaattg aaaatacaca gcttgaagaa ctcagccaaa aaattgcaaa aaatccagaa
    10561 aaacagaccg ctttaattaa cgatttttgg gataaagcaa aacaagccgg cacaccattg
    10621 attgaaccat tggacgaaca gcaaagccgt gtaacttttt tatggcgtgg agcaacgaaa
    10681 aatgtccgta tttggggcgg tgtgagtact gaacacgatt ttatgcaacg ctttggtgaa
    10741 acggatatat ggtataaaag ttatatagtg cccaatgaca ctttagtgga atacaaatta
    10801 gctccagatg tgccgacatt gcccgttgat gagaataccc aacgcagagc gttactggct
    10861 acggctcaag ccgatccact caataaaaca gcatacttcg agaaaatcgg acaaaatatt
    10921 cataataccg ataaattcaa ctacgcttcg ctacttaaat tgccaaacgc acctactcaa
    10981 tcttttttga ctgaagctcc aaatattgca aaaggtaaac tgcagcagtt gatttttgag
    11041 agtgcaacac tcggcaataa acgcagatta ttcgtctatc tgcctgacgg ttttagtgca
    11101 gaaaaaaact atgcggttct ctatttattt gacggtgttg aatataccga acgagtgcca
    11161 actaaaacca ttttagataa tatgatcgct cagaaagtga ttctgcctac tgtagcggtg
    11221 tttatcgaaa atccatcgag tgaatcaaga cgtgtcgagc tacctgccaa tcccatattt
    11281 acgcaagtat tggcaaaaga acttgtacct tttgttgaaa aacagctcaa tataaaaaca
    11341 aaggagcgaa ttattggcgg ctctagctat ggcgggctat cctctgctta caccgtactc
    11401 aactatccta atgtatttag taaagcattg attctatccg gttcattttg gtggcaaccc
    11461 aaaggcacac ctagtgaaca aagccactat attgctcaac aatttatcca aaaagaaaaa
    11521 ttaccactct gttttttcat cagtgcaggt ttttatgaaa gcgggaaatc ggctatttta
    11581 gaaacttctc gccatttaaa agatgtttta ctcgctaaag gttatccggt tacttatgtc
    11641 gaacaatcaa cctctcacgg ttatttagct tggcaagggc ttattagtga agggttaaaa
    11701 acattactgg gtagcaataa atgcacctca aattaagcaa tggtaaaaat cacccgattt
    11761 tcaaccgctt gtagctcaca aagccaacct tgctctttgg caagcagttc aaaatcggta
    11821 aggctactct gctgattgag taaaatttcc aagctatcgc cctttttaag atcgttcatc
    11881 gctttttttg ccataagcaa aggtaacggg caggcatagt cggttaaatc taaagaataa
    11941 ttcatcttca atacacaaaa ggcgaactca aggttcgtct tattcatcgc aattataatt
    12001 tacggcgatc cgccataatt ttacccattg ctgagagctt ttcttctgct aaatgctctc
    12061 gtcctagctc aaaaagcggt tcttcaatgg caattgcaca tcataacctg ccacaaaacg
    12121 ctcaattaag tctctatcca cattttcacg tttacctgcg agcagatctt caagctgtgc
    12181 cgacaaatcg tcccaatttt tatgtaaatg aacgtgctgg cgttccagtt cgtctacctc
    12241 tgcttgagct tgtggttgca ccttaataag ctcagggaag aaatcatctt cttcatcttc
    12301 gtgatgtaaa ggggcagaaa tgttgaagta attcaaaatc tgctgcacat cattcttgac
    12361 cgcttggttt accccgtttt tctcaaggta atccggcaaa atttgtagct gtttgcagaa
    12421 actttttact ttgctgtggc aagcataaag catatcgatc ggctctgccc agcttgcaaa
    12481 ggtttgtggt tcaagttgtt gcatatatac tccttgttat ttctgaccgt ttacttcaga
    12541 ttgtaaaggt ggcacttctt tgccaattcg agcataaaat tccacgacaa aatcatcaaa
    12601 acgatcttct tcgatggctt gacgaatttc tgccattaag cgttgatagt aacgtaaatt
    12661 atgaatggtg tttagtcttg cccctagaat ttcgccacat ttgtctaagt gatataaata
    12721 ggccttagta tagtttttgc aagtgtagca atcacattca ggatctaaag gtgtaatatc
    12781 gttacggtat ttcgcattac gaattttcac aatgccgttg gtcacaaata aatgtccgtt
    12841 acgggcattg cgggttggca ttacgcagtc aaacatatca ataccacggc gaaccccttc
    12901 gactaaatct tccggcttgc ccactcccat taaatagcgt ggtttatctg ccggaatttg
    12961 cgggcaaaca tattctaaaa tgcggtgcat atcttctttc ggctcaccta ccgccaaacc
    13021 accgactgca tagccgtcaa agccgatttc aactaatttt tccaccgaga ctttgcgaag
    13081 ttcttcataa ataccacctt gaataatgcc aaataacgca ttcgggtttt caagctcatc
    13141 aaagcgatca cgagaacgtt tcgcccaacg aagcgacatt tccattgatt tttttgcata
    13201 atcaaaggtt gccggataag gggtacattc atcaaaaatc atgacaatgt ctgaaccaag
    13261 atcgtattgg atctccattg atttttccgg cgaaaggaaa attttctcac cgctaatcgg
    13321 gttttggaaa gttacacctt cttctttaat tttacgcagt ttgcctaagc taaaaacctg
    13381 gaaaccgccg gaatcggtta aaatcggacc gtgccattgc ataaaatcgt gcaaatcacc
    13441 gtgcattttc atcacttctt gccccggtct taaccataaa tggaaggtgt tacctaataa
    13501 aatttgtgcc cctgtcgctt tcacttcttc aggagtcata ccttttaccg tgccataagt
    13561 gccaacaggc ataaatgccg gtgtttcaac gtgaaattcg cctttcgggc ggttaaaagt
    13621 taaacgccca cgacgagcgt taccacttgt tgtttttaat tcgtacttca ttttaatcca
    13681 caaataaaca gttcgtgaaa gcggtcaaaa accgccaaaa atttaccaat tagaatcgaa
    13741 agtcggtgcc gacacctaag ccgattccaa cgccgttaga gccaccaccg gctccaatac
    13801 tgccacccac accggttgat gagcaagcag ctaacactaa aatgccaacg cataataaga
    13861 gttttttcat tttttgctgt cctaaacgaa aaagagagtc tatgatagac ccaatgtatt
    13921 ctaacctgtt taatggatta aacctaattt taaagggtaa attatgaaaa ttttacgtaa
    13981 attcgctgct gtatgcgttg tctcaatggc tatgctctct gctaacggta atttactcag
    14041 cattagcgag caagaaatca acgactattt agccaccaaa ttagctgaaa aaatcccttt
    14101 acaggataaa gtgggcgttc cgcatttatt tgaattggat taccatctgc attcgctgac
    14161 aagccaaatt ggtagaaccg aagagaaaaa agtggctatt tccggtgttg ttgatgggct
    14221 tttaaaagcg aaaggcaaga aatatgatgc gtctatccag ttaaatatgg ataccacgcc
    14281 ttattacgat ccccaaaaag gggcattatt tcttaaagat gtcagattgt taagctggtc
    14341 tgctacaccg gctaaatatc aagcagaact gcaaatgttt ttgccggtat tggctgatgg
    14401 attaaatagt tttttaaaca gcacgcctgt ttatacttta gatgagaata aagcaaaaga
    14461 agcaatggtg aaaaagttcg gcaaagccat tgtgatagaa aatggtaata tcaagctaga
    14521 aactactatt ttttaaataa ttttgtgatc ttcgtctaat ttttacactt tgatagttga
    14581 gcttttacgc gttttattta caatccaaat atcgtcattc attatttgga gagttctatg
    14641 gatatcggta ttgttaaatg gtttaacaat gtaaaaggat ttggttttat tacatcggat
    14701 gtatgtgaag gagatatttt tgctcatttt tctgaaattc aagcagaagg ttatcgctct
    14761 ttaaaagtcg gccaaaaagt acagtttgaa ttagttcaag gagagcgagg tgcctcagcc
    14821 tcaaatatca cgcctataag cgaaaaaacg ttataaccca tcgttaataa aattattgtt
    14881 ataaccatac tttattagat tttaaagctc agcttttaca gctgagccat ttttgttttt
    14941 ttgaatatgc tttcccctta tcaattagtc ttaattaacc tactttatat cttcggtatt
    15001 ggtctaaata ttgtactgct taggtttctc agtttgcatt ttgatccatt aaataataac
    15061 ggtgtacgct tcctaattgg tggactcact ttattggttt ttattttctg gagattccgc
    15121 acatcgctgt ctcagctgat taaaactcca aaattgattg cgatttctct atttgtcggg
    15181 ttaatgatgt ccgcaaatat gtatttttgg ctaaaagggg tcaaaattac aaatgctgtt
    15241 accgcctcta tttttggggt attggcaatg ccatttggcg tgattattgc cgccatattc
    15301 tttcaagatg aaagagaacg cattaaaagc aaagctttct ggttcggctc aattttaacg
    15361 atcatcggtt cacttggatt tatttggcac gggaaaacac tctcgattgg cgaaggattt
    15421 attcttggtt ctatgttctt atttctgtca attattatcc gtaacataca aaacctagtt
    15481 gtaaaattcg ctcgagagct gaacgtattt acactaagtt gtgtaacatc tttgactact
    15541 tcactctcaa gtctattact tagccaacaa gcaggtaaat ttaatgagct ttttactgca
    15601 ccaaactggc tactattctc tctgatggct gctggtattt atgcgattat gatcggaatg
    15661 gtattaacct ttcacattat ccaaacccaa ggactgataa cctaccaagt attggaacta
    15721 ttaatgccgg tttcaacagc catcattgct tatattttac tggacgaacg tatttccatt
    15781 gttcaaatgg gctttgcttg tattgtgatt ttaggctcaa gtatcgcctt aaagttagta
    15841 aaatatccta agcactatta ggatctttgc taattaatat tggtaaaatt acatttggtt
    15901 tactaatctg ggcgttcatt agtaatgata aacaagcggt tattttttat tattttttgg
    15961 taaatactga tagtcatgta taaaaacctt tctttgcttt caccgactta tccatttgac
    16021 cttgaattca tcgccggtct tactcaatgt gagaaaggca actatcttga ttctattgtc
    16081 gataggcaac aagggatgca aggttatatg ttgcaattaa ctacttttgg gcatggctct
    16141 gtttctgatg gtaaacacgt cttttcagcc catcgaggtc aattactcct atttacaccc
    16201 aatgcagttc agcactacca tcgtcaccca gaaagccaat attagcacta taaatgggtc
    16261 tattttctgc ctaatccaaa gtggaataaa tggctgaatt ggtcaaatat ggaagaaaaa
    16321 atagggcgga tcaccattag cgatgaccgt tactttcagg aaatcagcca acttttcagt
    16381 aaaattgaat tagaacttaa atccagtaaa ttttttaaag aagatattgc aacaagtttg
    16441 ttggaatact tacttatgaa atgtatttcc gccgagaaaa ttgaggttat tcctgtcatt
    16501 gatcaacgca ttttgacagt ttgtgattta atcttaacga atttggctaa aaatgagagc
    16561 ttagaagcaa tggcagaaaa agtattttta tcgccatctc gcctctcaca tcttttcaag
    16621 caatcgctgg ggatcagctt agtgcaatgg cgggaattac aacgcatttc cgaatccaaa
    16681 aaattgcttt atttctctaa catctctatc tctaatattg cgaaatcctt aggctatgag
    16741 gattctctct acttttccaa aattttcaaa aagcataccg ggctgtcacc ctctgaattt
    16801 agaaccttag aacggcgtaa agacagtgaa atgtgatctt gatctcactt tactttgcat
    16861 agcagaattt tccatatcga taacaaaaag ttacctgttt ttctctcact caaaagcata
    16921 agatacaaac agctttaata gtgctgtttc tattagagaa gggatatcaa gctctgtgag
    16981 tgatattcag tgaaataact tcttatgtat gagggaagaa caatgaaaaa accaacgttc
    17041 aagcacgcat tattttcaag tattttagct ttagcctgtt acggttcggc atttgccaat
    17101 gatgttgtta atatctccaa aattgacggt atgccgtggt ttaatcgtat ggcagagggt
    17161 gttgtgcaag caggtaaaga taacaacatc aatgcttatc aagttggtcc atcaaatacc
    17221 gatgctccac aacaagtaaa gttaattgaa gatttaattg cgaaaaaagt tagtgctatt
    17281 tccatcgtcc ctaacgatgc aaatgcctta gaaccggtac tgaaaaaagc gaaacagaac
    17341 ggcattgtgg tattgaccaa tgagtcagta ggacaaccta gtgccgattg ggatattgaa
    17401 attatcgata atgcacaatt tgcggcagac tatgtagaag aagtagcaaa agctatgggc
    17461 ggaaaaggcg gctatgtgat ttatgttggt ggtttaaccg tgcctcaaca taatctgtgg
    17521 gctgatttat tcgtgaaata ccagaaagag cattatccag atatgtttga agtgaccagc
    17581 agaatgcctg ttgccgaaaa tattaatgat gcacgccgta ccaccattga tttagtgaaa
    17641 gcccattccg atcttaaagc ggtcgtttct tttggctcac aaggtcctat tggtgcaggg
    17701 ttggcagtga aagaaaaacg cttaaaaaat aaattaagtg tcttcggaat gatgattcct
    17761 tcccaagcag ccaatttaat taagagtggt gatattacga tgggcattac ctacgacccg
    17821 gcaaatgcag gttatgcttt agctgctgtc gctgccaaag tattaaaagg cgaaaaaatt
    17881 gaggacggat tagagatccc aacggttggt aaagccaaag tagatagtga gaaaaaacta
    17941 cttcaattcc acaatgtact gaaagtcacc aaggaaaata tcgataaatt atattaattt
    18001 gcgctgacat atgggctgat aaccagccca tattttcccc tcaggaggca cttatggcat
    18061 ttatttcgat gcggcatatc agtaaaacat tccacggcat aaaagcatta aatcaagtca
    18121 atctctcatt agattatggt gaagcacttt gccttgccgg tcaaaatggt tgtggaaaat
    18181 caaccttgat caaaattctt tccggcgttt atcaaccgga taaaggggct gaaattcaaa
    18241 ttggtgcaac ccaatatacc aagctgacac cacaggaatc gattgataaa ggcattcaag
    18301 tgatttatca agatttagcc ctcttcccca atttaaccgt ggcagaaaat attgccatta
    18361 acctgcaccg aaaatttggc tgggtgagtc aagatcacat tcatcaggtt gccctcactg
    18421 ctattcaaag cattaatgca aatctagatc tgaatgcaat attagaagat ttaccgatcg
    18481 ctcaacaaca gttagtcgct atttgtcgag cgttagcaca aaatgccaga ttattgatta
    18541 tggacgagcc gaccgcctca cttactgcaa aagaggtcaa agatctgtta gatgtcgtca
    18601 tcaaacttaa aagcaaaggc attagcatca tctttgtcag tcataaattg caagaagtaa
    18661 tgagtgtttc cgatagcgta ttagtgctga aagatggcaa tatggttggg caatatccta
    18721 tccgtgaaat gaatgaaaaa cgccttggtt tcctaatgac tgggctggaa atcgattaca
    18781 agcggttaga tttgccggat tttttgcaaa gaaaaaccgt attggaagtc cgcaatttaa
    18841 cgctgcctca tcagtatcaa gagattaact tttcgctacg agaaggggaa attgttgccc
    18901 ttacaggatt gctcggttca gggcgtactg agctttgcct aagtttattc ggcattactc
    18961 aaccacaatc cggcgatatt ttattaaacg gtgaaaaagt gatatttaac actaaccgtg
    19021 atgccattgc caaagggatt gcttatgtat cggaagatag aatgcacacc gggctgatta
    19081 tggcagagtc gattcatcac aatattattt cgactatttt tcacaaaatc accgataaat
    19141 tacagattat tcaatcctcg aaggcttatg caagtagcca agccttgatt gattcattaa
    19201 agattaaagt atccgattca gatttgcctg ttaataccct ttccggaggg aacgcacagc
    19261 gagtttcgat tgcaaaatgg ctggcaatcg accccaaaat tattatttta gatgccccga
    19321 ctattggggt cgatatcgcc aataaagaag ggatcttcca aattattaaa tcattggctc
    19381 agcaaggcat tgcagttatt tttgtgactg atgaagtgga agagacctac tacaacagcc
    19441 atcgggtaat tgtaatgaaa aaaggtcgca ttgttgatga aatcttgccg gcatattcca
    19501 gtgaaaaatc gattgcggag gttatttatg aaaatcagca ataatcatat tctttccagt
    19561 acgttactga ttttaatcct attattaacc ctatttaccc gtgattttgc aactattgag
    19621 aacctttatg atgttgtaaa taattacgca atgttaatga ttttagcttg tggtttattt
    19681 gtggtattgc tgtctggcgg cattgatatt tctttccctg ctatcactat tatttcccaa
    19741 tatgttatgg tcactctgat cggtaaatat gatgtaggat ttgccggcgt ttttgttatt
    19801 tcttccacca tcggcatatt attaggtttt attaatgctc tatttgttaa tcggcttagt
    19861 gtcccttcca ttattattac gatctcaaca ctcaatattt tttatggcgg actactctat
    19921 tttaccaaag gtgtatggct ttatgagtat cccgaatggt ttacccaaga aatcgtgttc
    19981 tttaaacaga taactgctga gggagtggaa tttggattat cgtttcaagc attaatggct
    20041 attttggcgg tgatattaac cggcttaatt accaataaac tcactattgg tcgaaaaatt
    20101 tacgctcttg gtggtaatcc ggatgcaacc tcccgtattg ggtttaatct cttatttctc
    20161 catctatttg catacggtta tatgggcttt atggcaggct tggcaggcat agtgcaatct
    20221 tataccgtac aatctgtcgc accggattca ctactgggtt atgaattaac cgtcctagct
    20281 gccgttgtat taggtggtgc tagtttatca ggtggtagag gcaccctgct tggtacagta
    20341 atgggcgtaa tgttactggc gattattcaa aacgggctaa atttaatgaa tgtatcgtct
    20401 tactggcaaa ccattattac cggtatcatc attatcttaa gtattagtgc aacagcctta
    20461 agtcagcgga aaaaacaagg agcataacga tgaaaagtaa actggatcca acgattttct
    20521 atctcttagc actcttcgca gttgcagtta tcgcctttag tattttcatt ccggatattt
    20581 tttggtctgt cagtaacttt caatctatgg catcacaaat tccagtatta ggtattttaa
    20641 ccctagcaat ggcattaccg atgttaacag gtggaataaa tctttccatt attgccggaa
    20701 tgaatgcttg ctcattaatt attgcctact ttattacgca atataccggt atttcttggt
    20761 tatttattgc tattttatta agcctttgct gtgccttagc cattggatta ctcaacggca
    20821 cattaattgc cattattcgt gtttcaccaa ttttggcaac cttaggcaca atgactttaa
    20881 ttaacggcat taatattcta gtcactaacg gttcaaccat tgcgaacttt ccggaaacaa
    20941 tgttgagtat taatgcttca acctttttat ggattccgct acccattcta atctttctct
    21001 ttcttgctgg gctgatttgg tttttcttag aaaaaactgc aatgggtaaa gcggtttatt
    21061 tcatcggcaa taatgaaaaa gccacctatt attcgggtat taataccaaa aaagtgctga
    21121 tttgggttta tgttatctcc tcaattcttt gtgtatttgc agctattttg atgatgtcta
    21181 aactcaattc tgcaaaagcc tcttatggtg attcctattt gttgatctcc attttagccg
    21241 cagtattggg cggtattaac cctgatggag gtaaagggaa actattaggg attatgctcg
    21301 ccttagtttt attacaagtg attgaaagtg gcttaaatat gctcggcatt agcagttaca
    21361 ttacaatgat tttatgggga agcttattgt tattctttat cttcctccaa aaaaatcgcc
    21421 ttttccgact aacacagtaa tggtggtaga ttatgcaaaa tgaaaaacaa ctcattgaac
    21481 aaggtaatac tgttattggt attgaattgg gctcaacccg aattaaagcg gtgttaatta
    21541 gctcagatgg gacgatttta gcaaccggtg gggcggattg ggaaaatcga ctgattgacg
    21601 gtatttggac ctatcatcaa aatgaaatat gggaaaaact acaagctgct tatatggatt
    21661 taagcaagac agtcaaagaa aaatatcaca caaccatgcg cacagccaaa gcattgggaa
    21721 ttagtgcgat gatgcacggc tacttacctt tcgataaaca cggaaatcag ctggttgcct
    21781 ttagaacttg gcgaaacaac attaccctaa cctcatcgga aaaattgacc gcactctttc
    21841 aatacccaat tccgcaacgt tggagtattg ctcatttaca ccaagcattg cttaaccaag
    21901 agcctcactt gccggaaatt gattacctga caaccctctc aggttatgtg cattggcaac
    21961 tgaccgatca caaagtgtta gggattggcg atgcttccgg tatgttcccg atagatactg
    22021 agttgaaatc ctacaatcca acaatgctgg ctcaatttga tcgagccatt gccgactacc
    22081 gtatgccttg gaaattggaa gcgattttgc cacaagttcg tgttgctggt gaacaagcgg
    22141 gcaaattaac cgaaaaaggt gcaaaattgt tagatccaac cgataattta caagctggca
    22201 ttccactttg tccacctgag ggtgatgccg gaacaggtat gattgcaacc aactgtatca
    22261 aagaaaaaac cggtaatatt tctgccggca cctccgcatt tgcaatgatt gtgttagaaa
    22321 aagccctatc caaagcctat tcagagctgg atattgtgac gaccccggca ggtaaattag
    22381 tggcaatggc tcacgctaat aattgcacct ctgatattaa tgcgtgggtg aatttattcg
    22441 gcgaatgctt atcggttttt ggcgtaaata tctgcaccga agaactttat gaaatgttat
    22501 ttctccatgc cttgcaaggc gatgcagatt gtggagggct attatcctac ggcttccact
    22561 ccggtgaaca taatgtcggt ttatccgaag gctgtccggt tttcttgcac ccaacgaatg
    22621 cccattttaa cttagcaaac tttatgcgaa cccatctcta taccgctttt ggtgcaatga
    22681 aattaggaat ggacattctc attaaccaag aaaaagtgga aatttcccgt attctcggtc
    22741 acggtggtat atttaaaaca gaaggtgttg cacaaaaaat tctgtcttcc gcactgaata
    22801 ttccattggc taccgcaagt actgcttcaa atggcggagc ttggggaatt gcattactgg
    22861 cagactttct cagcgtatca gaccgttacc gtttagaaga atatttagat aattgcattt
    22921 tcaacaccac caaactcaat ctggttcaac cggataaaac aatgagtgaa ggttatgaac
    22981 gctttatgca acgttataag caaggcattc ctgtggtaga aaaagcgctg tttttaaata
    23041 cagattaaat aatttaagta tcacaagcgg ttatttttgc caaaaacgac cgcttgttat
    23101 ttacatttgt acaaaaaata cctactataa agacgcattt gtaaaatgtg cattgatata
    23161 tcgcaagttt tgtgtatagg aaatgaacta attactgtat caatcagtcc ctttcttcat
    23221 cgttataccc tcttgcaaat ccgattaatc tatccttagt taaggtgttt tatgttagcg
    23281 atttggcaaa gttttaaaca aaattatcta atcaaatatt ggaatcctgt tgcagcagtg
    23341 atcgcagctg gtattatttc agcttattat ttcggcatta ctggcactta ttgggcggtt
    23401 acaggggaat ttacccgttg gggcggtcat ctgttaaatg ctttgggtgt tgatgtttcg
    23461 aactggagct attttcaact catcggctta gacggcacca tctttacccg cattgacggt
    23521 gtgatgattc tcggtatgtt tgccggctgt ctttctgctg cattatgggc gaacaacgtc
    23581 aaatggcgta atcagccaca taaaaaacgg attatccaag ccttgattgg cggtgcgtta
    23641 gccggtttcg gagcccgttt agcaatgggc tgtaacctcg cctctctttt taccggtatt
    23701 ccgcaattct ccgtccacgc ttggtttttc acctttgcga ccgccatcgg cacttacttt
    23761 ggggtaaaag tgactttatt accgatgttt agagtgaaat tagagctgaa aaaaggggca
    23821 gcaaaaatca aagaaaacga tccgaaacaa gctaatcgcc gtttttgggt tggtatgctc
    23881 atttttatca cttatctttc tgcttccctt tatgtaatgc aatcctcaat caaacttggt
    23941 tttgcaatgc tttgcggctt agcctttggc ttattgattg aacgagccca aatttgcttt
    24001 acctccgctt tccgtgactt atggattacc ggacgggctt atatggcaaa agcaattatt
    24061 ttcggcatta ttgccggcac aatcggcgtg ttctcttata ttcagttagg tgtttcacct
    24121 aaaattttgt gggctggccc aaatgccatt atcggcggct tattattcgg atttggcatt
    24181 gtgcttgccg gcggctgtga aaccggctgg atgtaccgtt caatggaagg acaggttcat
    24241 tttatgtggg tcggcgtagg caatgtagtc ggttcaaccc tgctggctta ctactgggac
    24301 gacttagctc cggtgcttac ccttgattat gaaaaaatca acctactcaa agaatttggg
    24361 ccggttggcg gattattcgt gaattatggc ttattaattc tttgcttaat tgctgtggtt
    24421 tggtgggaaa aacgcttcct tgccaaagcg aaggcaaaat tggcaactgc gtaatctttt
    24481 taatttattt tcaaccaaca agaggaaaat gactatggca ttcaccgttg tgcaaaaact
    24541 agataaagat ccgaaagaca tcacgcctga ttacacctta gataccttag gcgaaccttg
    24601 cccttaccct gcgattacga tgttagaaac aatgcctcaa ctacaaaaag gcgagatatt
    24661 ggaactgtta agcgattgtg cccaatcgat taataatatt ccgattgata ctaaaaatca
    24721 cggctatacc ctattaagcg tggaacaaga cggcactaaa ttacgctatt taattcagcg
    24781 ttaattgcaa aacattagtg aaaaacgacc gcttgtcgta aaatagcaac tattcaagag
    24841 ccttgtaacg caaggctcgt tttattctaa gaggtaaaag aaaaatgaat aaatacgctt
    24901 tacttgcaat attttggttt attattggca tttatgcttt actttttcgt gaaagcacca
    24961 atgatatacc accttttcca cattttgata aagtaagcca ttttgcctta ttctttatcc
    25021 agatttggtt atctgcaaac gcctttatcc gctcaaagaa agggatacct tatttcgggt
    25081 taataatttt tgccttactc tatgctttca ccagcgaatg ggctcaggca accttcacct
    25141 caaccagaga aggatcttgg cttgatggac tggcagatat gctaggtgca tcacttgcat
    25201 tattgctagt aaaatattgc aaaaaataaa gcataaaaga ccacttgtta tatggtttag
    25261 ccatactaca agcggtcatt ttttaccaaa attatgccat caactcatca atcaattctt
    25321 tcactgtttt aatgcggtca atcgctgaaa tgccattgcc caccgacaca tagccgtttt
    25381 caaaatcgcc ttccagcata cccaaacgca ttcctgtgct agcgttcatc actttggcga
    25441 tttcagaacg agttgcccca ttttcactca tttcaaagca ttttttcgct aattcagtcg
    25501 gcagtgaacg gtagtaatgt ggttcagtac ggaataacag cagatcattg gcatttgcct
    25561 ccacaatcca gtttttcact ttttcgcttg cacggcattc ttcggtggcg ataaatgccg
    25621 tgccaatcca cacgccttca gccccaagcg ttaaggcagc ctgcaccgct tttttatcgg
    25681 caataccacc agccagcatt atcggcaaat ccaccttgcc cacaaattcc gccagcacat
    25741 taaaactgcc tacctctttc atcggcaaag taccgccctc atcaaaaccc gtcagcacca
    25801 aaatatctgc tcccaatgct tgggctttta aagcgtccgc caaattgggg ttaggcaaac
    25861 ggcagatgat tttaatgcca gcagctttga atttggcgta aatctctggg tcaagccagc
    25921 cgttatccag caccacaggc acacgctctt caatgaataa atccaccatt aacggaatca
    25981 gcgaaaaatc ataagacaaa ataaatggta cgccaaaagg cttatcggtc aatgccttaa
    26041 ccttacgaat ctcgttacgc atacggtcaa gtacttcttc gttggaagtt ggattggtgg
    26101 gttgccctgc gtgtggggct aaaaaaccaa gcccaccagc ctcggcaacg cttgctacca
    26161 gatgagcgtc cgtcagccaa gacattggcg cttggacgat agggtgcttg atatttaaga
    26221 tttgggtaat gcggttcatt ggattttcct gtttgaataa aaagaattgg acgtagttta
    26281 atggttttgg atttgagagg gaatgcgtga aaatgggaag gtggttttcg gatttggaa
//
LOCUS       DANJWC010000014        26010 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.014, whole genome shotgun sequence.
ACCESSION   DANJWC010000014 DANJWC010000000
VERSION     DANJWC010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 26010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26010
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.014"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(126..476)
                     /gene="rplS"
                     /locus_tag="PWX02_000421"
     CDS             complement(126..476)
                     /gene="rplS"
                     /locus_tag="PWX02_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDL1728468.1"
                     /translation="MSNIIKQLEQEQLKQNLPSFRPGDTLEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTLRKVSNGVGVERVFQTHSPVIDSISVKRKGAVRKAKLYYLRE
                     RSGKSARIKERLGD"
     gene            complement(502..1257)
                     /gene="trmD"
                     /locus_tag="PWX02_000422"
     CDS             complement(502..1257)
                     /gene="trmD"
                     /locus_tag="PWX02_000422"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDL1728469.1"
                     /translation="MWIGIISLFPEMFKAITDFGVTGRAVKQNLLQIECWNPRDFTLD
                     KHKTVDDRPYGGGPGMLMMVQPLRDAIHAAKKAASAVDGGEAKVIYLSPQGRKLDQTG
                     VQTLASNQKLILICGRYEGVDERLIQTEVDEEWSIGDYVLTGGELPAMTLIDAVARFV
                     PGVLGKQASALEDSFAEGLLDCPHYTRPEVLDGMPVPEVLMSGHHEQIRKWRLEQSLE
                     RTWLRRPELLDSLALTDEQRVLLAKIKKQHKIS"
     gene            complement(1302..1829)
                     /gene="rimM"
                     /locus_tag="PWX02_000423"
     CDS             complement(1302..1829)
                     /gene="rimM"
                     /locus_tag="PWX02_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDL1728470.1"
                     /translation="MSEQKIEVVGKLGSTYGIRGWLRLYSSTEQSESIFDYQPWFLKI
                     KGNWQPVELESWRYHSNDLIVKLKGTEDREAAQLLTNAEIGVDLSVFPELEEGDYYWH
                     DLIGCTVVNLEGYTMGTVTEMMETGSNDVLVVRANSKDAFGKQERLIPFLYEQVVKRV
                     DLATKAITVEWDAGF"
     gene            complement(1865..2113)
                     /gene="rpsP"
                     /locus_tag="PWX02_000424"
     CDS             complement(1865..2113)
                     /gene="rpsP"
                     /locus_tag="PWX02_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDL1728471.1"
                     /translation="MVTIRLTRGGAKKRPFYQIVVADSRSPRDGRFIERIGFFNPLAA
                     GQAERLRLDVAKVDAWVAKGADLSDRVASLVKEARKAA"
     gene            2378..2620
                     /locus_tag="PWX02_000425"
     CDS             2378..2620
                     /locus_tag="PWX02_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1728472.1"
                     /translation="MSDKSIILEIINALGNKENIKQVEACLTRLRVVLHNNGLLNKSA
                     LKKLGAVDVIKVGDTQQIIFGAKAGQYRDEINALIG"
     gene            complement(2664..3488)
                     /locus_tag="PWX02_000426"
     CDS             complement(2664..3488)
                     /locus_tag="PWX02_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL1728473.1"
                     /translation="MNGPTQLAHFQEQIRKEYDGLSKRLKQVAQYVLDNGNSVVFDTV
                     STIAERADVPPSTLIRFANAFGFSGFNEIKQLYRQDMMEGTSRYTERVQLFRQIEPLD
                     EQNSELNHILDVFVQGNTQALQQLSTQISEEQLAQTVDILHNAKRIFIVGLKRSFSIA
                     NYLNYALHHLDYDVFMIDGSGGMFDEQLSRIREGDAVIAVSFSPYANETLNVVTATAR
                     PGVKHIAITDSQLSPLLSFSDVSFIIKEAQVSGFRSQCATMTLAQTIAVSLALKKA"
     gene            complement(3626..4966)
                     /locus_tag="PWX02_000427"
     CDS             complement(3626..4966)
                     /locus_tag="PWX02_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1728474.1"
                     /translation="MSVSQRFINFMNTHVAPVARRMENQPHISAIRDGFIVVLPFLIV
                     GSFIMILLIPPFDENTQNAFGQAWWRFANWASPYGWNFFQMSFNAISLFTSASIAYNL
                     AKAYKREPLPAAFLSVMAFLLVAAPVKDGMMDIKFFGGIGLFSAIFIAIYSVEMTRLL
                     EFLKIKIRLPKEVPHAVAESLNIVIPILAILLTLYPFAIWVESATGRNIPQLIMDFMA
                     PLIAVSDSLGAICLFVIATHLLWFLGINGSLVLMQLWTPFLLQNMAANLAAFQAGEPL
                     PFIITNSFWDFYIVHGASGGVIALAFLLVRSKSAHLRSIGKIGLVPSFFSIGEPIVYG
                     VPMVVNPLFFIPLIFAPLANAVIAYLILDFNLIHRVYLMAPWTTPAPIGAYLVSAGDI
                     WAPILSIALIILDIVIYYPFFKMYEKVCVEKEKAQNLDEKAQQELIEKARMAAQ"
     gene            complement(4969..6309)
                     /locus_tag="PWX02_000428"
     CDS             complement(4969..6309)
                     /locus_tag="PWX02_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HDL1728475.1"
                     /translation="MSCNTSPKRFHASWESIKQHQVPEWYEDAKFGIFIHWGIYSVPA
                     FAPPTCQLGEIDIDEQWFCNNPYAEWYFNSINVKKGPTYEHHIKTYGESFAYNDFLPL
                     WKAEKWQPAEWAKLFAKAGAKYVVLTTKHHDGFCLYPSKYTDFNCMQAGPKRDLMGEL
                     TENVREQGLKMGAYYSGIIDWTYSSAPIFTESQNFSNACPTYEYADYAYKQVVELIDR
                     YKPDVLWNDIGWPKVGEHMLPHLFAHYYNSVQEGVVDDRWNKLWCDFTSKEYKHGVAS
                     RDKKWEMCRGMGLSFGYNQVEDESHLISVKDLISLLVETVADNGNLLLNIGPKADGTI
                     PQEQVERLLALGSWLEINGEGIYKSRCSQHQTEQIGDVTYRYTKVGADLYLYIDGLTE
                     AQISLPLSVYGELQALNTDLKFTIENTAQGRVLHIQNYQPDWYVLGFKIADGEK"
     gene            complement(6598..8514)
                     /gene="iolC"
                     /locus_tag="PWX02_000429"
     CDS             complement(6598..8514)
                     /gene="iolC"
                     /locus_tag="PWX02_000429"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HDL1728476.1"
                     /translation="MATVNHQDRPLDLICLGRVAVDFYGQQIGSRLEEMTTFSKYLGG
                     SSGNVAYGTAVQGLRSSMLARVGDEHMGRFLREELQSVGVDTSHLITDKERLTALVIL
                     GIKDKETFPLIFYRDNCADMAITKDDFSEAYIASSRCLAITGTHLSNPKTREAVLTAL
                     EYAGRNEVKRALDIDYRPVLWGLTSLGDGETRYIDSAEVTKSLQEVLHHFDLIVGTEE
                     EFHIAGGSTDTLTALKNVRKVSNAVLVCKRGALGCSVFEGELPNDLDGGLNVYGVRVE
                     VLNVLGAGDAFMSGLLRGYLNNEGWEQACHYANACGALVVSRHGCSPAMPTKRELDDY
                     LTRAESVPRPDLDERLNHLHRVTTRKKQWENLCIFAFDHRKQLVDMANKVGADLARIP
                     KLKQLLLKAMEQTATQSGLPQNQAGILADTTFGQDVLNEITGKDYWIARPIEEPSSRP
                     LELEYGDLGSQLASFPRDHVIKCLAFYHPNDESGLKARQDRKLKEVYQACCRTGHELL
                     LEIILPADMPQSEAFYNQAIAHFYQLGIKPEWWKLPDVSRAQWQAISATIEANDPDCR
                     GILILGLDAPLEVFKDTFEQAKGCEKVKGFAVGRTIFGEPSEKWLAGELDDNTLISAV
                     SEKYRTLIDLWQQR"
     gene            8782..10716
                     /gene="iolD"
                     /locus_tag="PWX02_000430"
     CDS             8782..10716
                     /gene="iolD"
                     /locus_tag="PWX02_000430"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HDL1728477.1"
                     /translation="MQTTRLTVAQALVQFLDNQYLDVDGKEIKFVHGVAAIFGHGNVL
                     GIGQALAQNAGGLTLYQGRNEQGMAHLAMGFAKQNLRQRIIACTSSVGPGAANMVTAA
                     ATATANRIPLLLLPGDVFATRQPDPVLQQIEQPYDLSISTNDAFRAVSKYWDRVQRPE
                     QLMSACINAFRVLTDPAETGAVTIALPQDVQAESFDFPNSFLQKRVHRLERTPPTTAM
                     IKQAVTLIQTKKKPLIVCGGGVRYSQAGQILQKFAKQFGIPFAETQAGKSAVVSDHAL
                     NVGGVGETGCLAANRLAKEADLVIGIGTRYTDFTTSSKWIFQNPNVQFLNINVARFDA
                     YKLDGVQLVADAKTALEALSQAIPTDYQAQWENEISQAKADFADEIQRLRAVQYHPDF
                     TPEINDNLDHKAVHNEFIQLTGSQLTQTQVLGIVNETIDNNAIIVAAAGSLPGDLQRT
                     WLSKGEETYHLEYGYSCMGYEVSAAVGVKIARPEQEVYALLGDGSYLMAHTELVTSIQ
                     EGKKINVVLFDNMANGCINNLQIGNGMDSFGTEFRFRNGETNQLNGGLVPIDFAKNAE
                     AYGCKTYRVTNEAELRAALDDAKKQTVSTLIDIKVLPKTMAHGYGCWWHVGVAEVSES
                     ERIQQARQRSEEQLAKARKY"
     gene            10843..11739
                     /gene="iolE"
                     /locus_tag="PWX02_000431"
     CDS             10843..11739
                     /gene="iolE"
                     /locus_tag="PWX02_000431"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011817366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HDL1728478.1"
                     /translation="MKAENIQLGIAPIGWTNDDLPELGAENTFEQCVSEMALAGYTGC
                     EVGNKYPRDVAVLKHKLDVRGIQICNAWFSTFFVDGKREETIAEFIKHRDFLHAMGAK
                     VIGCSEQSRSIQGTTKSVFKEKPIFNDEDWKRMAEGYNELAKLAAEKGMKVCLHHHMG
                     TGIQTPEEVDRYMAEVNDDVYLLFDSGHLYYSEGCQKAMLGVLEKYIDRIIHVHLKDV
                     RDEVVAEVKAKDLSFLEGVKKGTFTVPGDGVIDFRPIFDILEKHDYKGWMVVEAEQDP
                     ALANPFEYALKGRKYIRETAGV"
     gene            11741..12751
                     /gene="iolG"
                     /locus_tag="PWX02_000432"
     CDS             11741..12751
                     /gene="iolG"
                     /locus_tag="PWX02_000432"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005537901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HDL1728479.1"
                     /translation="MLKVGIIGAGRIGRVHSESISKYVKGAEIKAISDVKITEELTAW
                     AKSMGIPEVYDDYKKILQDPEIDAVLVCSSTNTHAPISIEAAQAGKHVFCEKPVDADP
                     AKIREVLSAVEKAGVKFQVGFNRRFDHNFKAIKDRVAAGDIGEPHVIRVTSRDPEAPP
                     IEYVKVSGGMFFDMTIHDFDMIRYLSGSEVEEVFAVGTVLVNPEIGKAGDIDTAVITL
                     KLKNGAIGVIDNSRKAAYGYDQRAEVFGSKGAIHITNDTGSTAVFSGENGVVAEKPKY
                     FFLERYMQSFADEISCFVDSVVNDKPTLVNGNDGLQPVLIALAAKKSLEENRPVKLSE
                     VA"
     gene            complement(12873..13064)
                     /locus_tag="PWX02_000433"
     CDS             complement(12873..13064)
                     /locus_tag="PWX02_000433"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728480.1"
                     /translation="MAKEWGNYPLGIRFTFEYIGMQTENFKYFIGIVLTLLIIPAFYL
                     FKQSIKIEHDYIYFPKDNK"
     gene            complement(13130..13549)
                     /locus_tag="PWX02_000434"
     CDS             complement(13130..13549)
                     /locus_tag="PWX02_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728481.1"
                     /translation="MSSIKEIFSTKNSPINVLMQTEWFLGFILIVVYLDAYFQLVHQV
                     PIYIAIQQKEIYLTKTIDYIAAIFFFSVTFTALFTMMRLLHIFILSDLLDKIGCNTYE
                     LPKIEDIHKYEMLSILKIQAAKENNAELRYYVKKKKK"
     gene            complement(13619..14752)
                     /locus_tag="PWX02_000435"
     CDS             complement(13619..14752)
                     /locus_tag="PWX02_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL1728482.1"
                     /translation="MKTIKIGLVGTGYIGRCHAIAYAQAPTLFPLKGKIQLEYLAEIN
                     QALADQKAREFGFQRATDDWRKLVTDPNVDVVDICTPNFLHKEIALEAIKHGKHVYSE
                     KPLALTAQDAKEMVIAAQQAGVKTLVGFNYIKNPTTQLARQIIQNGEIGEVVHFYGTH
                     NEDYLANPNTPIDWHCYKAKAGLGTLGDLAAHIVSIAHYLVGSDIERVIGDMQTVIEE
                     RPSPHNPAEKIKVENEDQASSLIRFANGVMGTLESSRISCGRKMGLTYVVTGTKGTLS
                     YTQERMAELKLYLHDDDKSRQGFKTILVGPEHPDYAAFCVSAGHGIGFNDQKAVEIRD
                     LINGIAADDPMYPDFEEGYKISRVLEAIAKSAEEKRWVEVSEI"
     gene            complement(14883..15911)
                     /locus_tag="PWX02_000436"
     CDS             complement(14883..15911)
                     /locus_tag="PWX02_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1728483.1"
                     /translation="MTTQFLKNMGMVDDKGKIDFIAFFERFGVLIFLILLIIFFTSQN
                     GAFLSQRNIYNVLTEVSIFGIMAVGMTFVILTAGIDLSVGSILAVTAMFAAYIIKGDN
                     TVTLEAGAWGGMSWLIGLSICLGLGTFIGWLHGLGVTKLKLPPFIITLGGMTIWRGLT
                     LVLNNGSPIAGFDEGYRWWGRGDILGVPVPVVIFAVVAIIGYFALHKTRWGRYVYSVG
                     GNPEAARLAGVNINRTLVSVYVVIGALAGLAGFILSARLGSAESVAGVSFELRVIASV
                     VIGGTSLMGGYGRVTGTIIGSIIMGVLINGLVLMDVSAYYQQIIMGLIIILAVAFDTY
                     AKSRRGAL"
     gene            complement(15923..17467)
                     /locus_tag="PWX02_000437"
     CDS             complement(15923..17467)
                     /locus_tag="PWX02_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL1728484.1"
                     /translation="MSTPLLEVKDLTKSFSGVMALNGVKLTVGKGEVHALLGENGAGK
                     STLLKALSGAQPQTSGEIIFNGETLSLSDSPFDRQLKGIVTIYQEFNLLPNMTVAENF
                     FLGREPVKGLFVNEKAVNEEAQLVLDNLGLNIKPDTQVSRLSVAQQQMVEIARAMTLN
                     AKLIIMDEPSAALSDKEVETLHQIVRDLKSRGISVIYVTHRLNEVFALCDRFTVFQDG
                     RYSGEGEVKNVNVDQIIKMMVGREVAFTRRPPEETHHTHRPIRLAVKNLHREKPAFDP
                     HGIALHGVSFNIHEGEVLGIAGLVGAGRTEIARCLFGVENYTADEISLDGKPYAATSP
                     LDALEKGVALVPEDRKKEGLVLGLPIKTNMTLPILSKLLKGNLFVDNQKEDDLIETYR
                     QALRIKMASSELEARKLSGGNQQKVILARCMALNPKVLIVDEPTRGIDVGAKSEVHQV
                     LFDMAKQGVAVLVISSDLPEIMAISDRIITLSEGRVTGEIHGDDATEEKLMSMMAVGV
                     QRKAAA"
     gene            complement(17518..18456)
                     /locus_tag="PWX02_000438"
     CDS             complement(17518..18456)
                     /locus_tag="PWX02_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL1728485.1"
                     /translation="MKKRIKFVLSALTLGLAMNSFAKDELVVFSLPNLSSPFEVQLQK
                     VAVETSKKLEIKLQVLDGQSSSTKQASDLENAITRGAKGIIISPNDVNAISGAVEEII
                     KEKIPAATLDRKVESSKPVPHFGANNYTGGQEVAKAVKAKYPNGAKIILLTGQPGSTS
                     NIERTKGIRDELAAGGDKYKIVVDQTGNWLRSEGLRIIESVLPTLKEKPEVIISANDD
                     MALGAIEALRSQGLKAGDILVTGFDSTPEALARVKDGWLYLTADQRPSFAVSTALEQV
                     VGNIRESKEVSGADYPPKIILKDNLQEAERFAEISE"
     gene            complement(18788..20299)
                     /locus_tag="PWX02_000439"
     CDS             complement(18788..20299)
                     /locus_tag="PWX02_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDL1728486.1"
                     /translation="MSQFVQNFINGAVVESQSQRVTSVFNPATGEETKKVKLSLPNEV
                     DAAIAVADQAFKSWSQQSPLRRARILFKFKELLEANFDELARLISSEHGKIYSDAIGE
                     LTRGLEVVEFATGIPHLQKGEFSANAGRGIDIHSIQQPLGVVAGITPFNFPAMVPMWM
                     FPVALACGNTFVLKPSEKDPSVSIRLAELLKEAGLPDGVFNVVQGDKEAVDILLTDSR
                     IQAVSFVGSTAIAQYIYEKGSANGKRVQALGGAKNHALIMPDADVQGTINALLGAAFG
                     AAGERCMALSVAVVVGDELADKIIEELIPKVKALKIGSGLLPQGVLENDMGPVISKEH
                     KAKIESYIDQGVAQGAKLCVDGRGYKVEGFENGYFVGGTLFDNVTPEMTIWQDEIFGP
                     VLSVVRVKDYAEGIALINSHQYGNGSAIFTADGDSARQFTQDVQAGMVGVNIPIPVPM
                     AFHCFGGWKASIFGPLNVYGTDGVRFYTRMKTITTRWPDSSVRSQAAFNFPTL"
     gene            20518..21342
                     /gene="iolB"
                     /locus_tag="PWX02_000440"
     CDS             20518..21342
                     /gene="iolB"
                     /locus_tag="PWX02_000440"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HDL1728487.1"
                     /translation="MSSPLLSKPNFSQPEGLIQHITPENAQWEYVGFDVYQLSKQQIT
                     LESGENELCLVFVTGFGSVYVDDYTFENIGNRTTPFEKIPPYAVYIPPHKSVLIKTEL
                     NLELAVCKAPSQGHYPVRLISPNHIGVEKRGFGNNQRLVHNILPETEPADSLLVVEVF
                     TDEGNTSSYPSHKHDDKHSTEETYLEETYYHRFDPPQGFAMQRVYTDDRTLDECMAVY
                     DKNVVKVPRGYHPVATIAGYNNYYLNVMAGPVRKWKFTWEQDHLWVNSETYKAKFE"
     gene            complement(21418..22869)
                     /locus_tag="PWX02_000441"
     CDS             complement(21418..22869)
                     /locus_tag="PWX02_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDL1728488.1"
                     /translation="MPNHKFLLLRFQAYINGKFVSSQNGKTFTVQNPTNGEKIGEVAD
                     LGTEETAQAIEAAAVAQKKWQALLPKERSVILRKWFELIMQNQEELAQILSLEQGKPL
                     AESRGEIAYGASFIEWFAEESKRIYGDVLPQDKAGRRLFVTKQPIGVVAAITPWNFPN
                     AMITRKAAPALAAGCAIVIKPAAETPLSALALAQLADQAGIPKGVFNIITSSDAEGIG
                     RELSTHPTVRKLTFTGSTRVGKILMQQSAGTVKKLALELGGNAPAIVFDDADLDTAVE
                     GVFASKFRNGGQTCVCANRIYVQAGIYEQFLAKLSEKVAQIKLGSPFEEEVTMGALIS
                     ERAVEKVESHINDALSLGAELVLGGERHALGGTFFQPTILKNVTQEMKVAREETFAPL
                     APIFKFETEEEVIQWANDTEFGLAAYLFSQNIGRIWRVSEQLEYGIVGINEGIISNEI
                     APFGGWKESGLGREGSKYGIEEFLEIKYLCMKV"
     gene            complement(23047..24357)
                     /locus_tag="PWX02_000442"
     CDS             complement(23047..24357)
                     /locus_tag="PWX02_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDL1728489.1"
                     /translation="MIYAEIFVVLAAIYLGLRSGGLGIGLYGGLGLAILTLGFGLPMG
                     SIPVDVMLIIMTVVIGAAVLQAVGGMDLLVRYAEVLMRKNPKYINFIAPMITWLMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRALAGSVVSSQLAIAGSPLSAAMAAMIAVMEN
                     NSVTFFQVIAVCLPVSFIASMVVAFVASKQGCELQNDVVYLERLKAGIVSPSRKLASE
                     STYSKGAKLLIGIFLLATVLVVLLAAVPGLKPVYENGKSMSTRDIIIVMMLASSCLMM
                     LVGKIKPDAIVASPIFRSGMTSIAVIIGIVTLGMTFVDAHLIEIKASIGNILTEYPML
                     LAVVLFFTCALLYSQGSTSALIIPLAVSLGIPNWAILASFIAITGIFILPTYPTSLAA
                     MELDTTGSTRAGKYIIDHPFMLPALVGVAVGLAFGFVWAPMIVS"
     gene            complement(24373..25068)
                     /locus_tag="PWX02_000443"
     CDS             complement(24373..25068)
                     /locus_tag="PWX02_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HDL1728490.1"
                     /translation="MARNIIGILGGMGPAATADMFQKFIRLTPADCDQAHIPLLISSI
                     PDIPDRTRYILANGENPAPVMEKYIEGLESAGATCIIIACNTAHYWFEHLKAKSQVEM
                     LSMIDCVVDEVLKCGKRRVGLLATDATLAVELYKKQLEAQGLTFICPTENGQKAVMDS
                     IYLLKSGELEKATELMLQQRDELIRLGAEVIILGCTEVPIILAQEIEREPMRYVDSTL
                     VLVKKAIDWYQTH"
     gene            25284..25955
                     /locus_tag="PWX02_000444"
                     /pseudo
     CDS             25284..25955
                     /locus_tag="PWX02_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250010.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         7580 a         5814 c         5265 g         7351 t
ORIGIN      
        1 aacattataa caaaaaagaa agcaagattt gtaaatcatt gaggaaaaat aactgcttgt
       61 aaatacaaaa agcaaaagcc ttgagtttcc tcaaggcttt tgtgcatttc actgttttgt
      121 agcaattagt cgccaagacg ctctttgata cgtgcagatt taccagaacg ctcacgtaag
      181 tagtaaagtt tagctttacg taccgcacct ttacgtttaa cagaaatgct gtcaattaca
      241 ggtgagtgag tttggaatac acgctcaacg cctacgccat ttgaaacttt acgtagagtg
      301 aatgcagagt gcaagccacg gttacgaatt gcaataacca cgccttcgaa cgcttgcaaa
      361 cgttttttac taccttctac tacccatacc ttaacttcta atgtgtcacc tggacggaag
      421 ctaggtaagt tttgttttaa ctgttcttgt tcaagttgtt tgatgatgtt actcattttg
      481 aaaaccttct aaatcctaga attaactgat tttatgttgc tttttaattt tagctaacaa
      541 cactctttgt tcgtcagtca gagctaggct atccaatagc tcagggcgtc ttaaccacgt
      601 tcgctcaagc gattgttcca atcgccattt acgaatttgt tcgtgatgtc cagacatcag
      661 cacctccgga acaggcatac cgtctaatac ttcagggcgg gtatagtgtg ggcaatctag
      721 taatccttct gcgaaagaat cttctaacgc tgaagcctgc ttacccaata cgcccggaac
      781 aaatcttgca acagcatcaa ttaatgtcat tgcaggtaat tccccccctg tgagaacgta
      841 atcaccgatt gaccattctt catcaacttc tgtttgaatc aatcgctcgt ccacgccttc
      901 atatcgtcca caaattaaaa tcagtttctg atttgaagcc agcgtttgca cgcctgtttg
      961 atcgagttta cgtccttgcg gtgaaagata aatcacttta gcttcaccac catctactgc
     1021 tgatgctgcc ttttttgctg cgtgaatcgc atcacgcaac ggctgcacca tcattaacat
     1081 tcccggccca ccgccataag gacgatcatc aaccgtttta tgtttatcta atgtgaaatc
     1141 acgtggattc caacattcga tttgcaatag attttgctta actgcacgtc ctgtcacccc
     1201 gaaatcggta attgctttaa acatttcagg aaacagtgaa ataataccaa tccacatctt
     1261 aacgcctcaa atgttttcca aagctcaccg catacctgag gttagaaacc agcgtcccac
     1321 tccaccgtta tggctttggt ggcgagatct actcttttaa ctacttgttc gtacaaaaac
     1381 ggaattaatc gttcttgttt accaaaagca tctttgctgt ttgcacgcac cactaataca
     1441 tcgtttgaac ctgtttccat catttcggtt acagttccca ttgtgtagcc ttccaagttt
     1501 actactgtac agccgattaa atcgtgccaa tagtaatccc cttcttccaa ttcaggaaat
     1561 acagataaat ctacaccgat ttcggcattc gttaataact gtgcggcttc acgatcttcc
     1621 gtacctttta acttcacaat caaatcgttg ctgtgataac gccaactttc taattcgact
     1681 ggctgccagt tgcctttaat ttttaaaaac cacggctgat agtcgaaaat gctttcagat
     1741 tgttctgttg atgaatagag gcgtaaccag ccacggatcc cataggttga acctaattta
     1801 ccgacaactt caattttttg ttcgctcata ctcacctact tatttaattt tggcttattt
     1861 aatattaagc tgctttgcga gcttctttta ctaaagaagc aacacgatct gataagtcag
     1921 cacctttagc aacccacgca tccactttag ccacatctaa acgtaaacgc tcagcttgac
     1981 cagctgctaa tggattgaag aaaccgatac gctcgataaa gcgaccatca cgaggtgaac
     2041 gactatccgc taccacgatt tggtaaaatg ggcgtttttt agctccgcca cgagttaaac
     2101 gaatggttac cataaccttt cctctaaatg ttgatagtaa aaaataaacc ctcgttcgag
     2161 gtgagggtga cgaacttgtt tcaaaagaga aaacaagcgg tcatattata cagatttttt
     2221 gcggaaagac aagggagatt cattgatatg actcttttaa agaaagatac atttagcgtt
     2281 tttttataca aattactaat aaaaattgtt ctaaccatca gaaaatgttt aaaatatcat
     2341 aataagagtt ttaaatataa ataggtatgg aggaaatatg tctgataaat ctatcattct
     2401 agaaattatt aatgctttag gaaacaaaga aaatatcaag caagttgaag cttgtttaac
     2461 ccgtttacgt gtggtgttac ataacaacgg tttattaaat aagtcggcac tgaaaaagct
     2521 tggggcggtt gatgtcatta aagtcggtga tacgcagcaa attatttttg gtgcaaaagc
     2581 cgggcagtat cgtgatgaaa ttaacgcttt aatcggctaa taaccaaaga cctgcattta
     2641 cgcaggtctt tcactttctt aatctaggct tttttcaacg ccagtgaaac ggcaatcgtt
     2701 tgtgcaagcg tcattgtagc acactgggat cgaaaaccac tcacctgtgc ttccttaata
     2761 ataaacgaca catcgctaaa tgaaaggagc gggctaagtt ggctatcggt gatcgcaatg
     2821 tgtttcaccc ccggtctggc ggttgctgtc accacattga gcgtttcatt cgcatacgga
     2881 gaaaagctca cggcaatcac cgcatcacct tcacgaattc ggcttaattg ctcgtcaaac
     2941 ataccgccgg aaccatcaat cataaaaaca tcataatcca aatggtgtaa cgcatagttt
     3001 aggtagttcg caatgctgaa tgagcgtttt aagcccacaa taaaaatgcg tttagcattg
     3061 tgtaaaatat ccaccgtttg tgctaactgc tcttcgctga tttgtgtcga taactgttgc
     3121 agtgcctgcg tattaccctg tacgaacaca tctaaaatat gattgagctc actgttttgc
     3181 tcatcaagcg gttcaatttg acgaaaaagt tgcacacgtt cggtgtaacg tgatgtgcct
     3241 tccatcatat cttggcgata aagctgttta atttcattaa aaccgctaaa tccaaatgca
     3301 ttcgcaaaac ggattaaggt tgaaggagga acatcagccc gttcagcaat ggtagaaacc
     3361 gtatcaaaca ccacgctgtt gccattatcc agcacatact gtgccacttg ttttaaacgt
     3421 ttacttagtc cgtcatactc ttttcggatt tgttcttgaa aatgtgctaa ttgagtaggt
     3481 ccattcattt caaattatct ctgcttgaat tttttatttc attatacgct tttcttgttt
     3541 acaagcggat aaattttgtc aattttttgc aaaaataagg gctaatcttt cgattaaccc
     3601 ctctcaatta cgctagaaat aagtgttatt gtgctgccat tcgtgctttt tctatcaact
     3661 cctgttgtgc tttttcatct agattttgtg ctttttcttt ctcgacgcag actttttcat
     3721 acattttgaa gaatggatag tagatcacaa tatccaaaat aatcagtgca atacttaaaa
     3781 ttggtgccca aatatcaccg gcagaaacca gatatgcccc aatcggtgcc ggtgttgtcc
     3841 acggtgccat caagtaaaca cggtgaataa ggttaaaatc taaaatcagg taggcaataa
     3901 ccgcattcgc tagtggtgcg aaaatcagcg ggataaagaa cagcggatta accaccattg
     3961 gcacaccata cacaattggt tcaccgatag agaagaagga cggaaccaag ccgattttac
     4021 cgatagagcg taagtgtgcc gatttactgc gtaccagcag gaatgccaag gctattacac
     4081 caccacttgc cccgtgtacg atatagaaat cccagaagga attggtgatg ataaagggga
     4141 gcggttcacc agcttggaat gctgctaagt ttgccgccat attttgcagt aggaatggtg
     4201 tccaaagttg cattagtacc aatgagccgt taatgcctaa gaaccagagc aaatgggtag
     4261 caataacgaa caagcaaatt gcccctagcg agtcactcac agcaatcaat ggcgccataa
     4321 agtccataat tagttgtgga atattacgac ctgtggcact ttccacccaa atggcaaacg
     4381 gatagagagt cagtaaaatc gctaaaattg gaatcacgat attcagagat tctgctacag
     4441 cgtgcggcac ttctttcggt aagcggattt taatttttaa gaactccaac aagcgggtca
     4501 tttctaccga ataaatggca ataaaaatcg cagagaatag cccaataccg ccaaagaatt
     4561 taatatccat cattccgtct ttgaccggtg cagccactaa caaaaatgcc attaccgaga
     4621 gaaatgccgc tggcaggggc tctcttttat aggctttggc aaggttgtag gcgatactgg
     4681 cagaggtaaa taatgaaatc gcattaaacg acatttggaa gaaattccaa ccgtaaggac
     4741 ttgcccagtt agcaaaacgc caccaagctt gaccgaaagc attttgggta ttttcatcaa
     4801 atggcggaat tagcaaaatc ataataaagc tgccgacaat taagaatggt aacactacaa
     4861 taaaaccgtc tcgaatagcg gaaatatgcg gttgattttc cattcggcgt gccactgggg
     4921 caacgtgggt attcataaag ttaataaagc gttgtgaaac agacatacct atttctcccc
     4981 atctgcaatt ttaaagccta atacatacca atccggttgg tagttttgaa tatgtaacac
     5041 tctgccttgt gcagtgtttt caatggtaaa tttcaggtcg gtattgagtg cttgtagctc
     5101 accataaact gacagtggca agctgatttg ggcttcggtt aagccatcaa tgtaaagata
     5161 gagatccgcc ccaactttgg tataacgata ggttacatca ccgatttgct cggtttggtg
     5221 ttggctacaa cgggatttat agattccctc tccatttatt tccagccagc tccccagtgc
     5281 taataagcgt tcaacttgct cttgcggaat agtgccatct gctttcgggc cgatattcag
     5341 caataaattg ccgttgtcgg ctacggtttc taccaatagg gaaatcaagt ctttcaccga
     5401 aatcaaatgg ctttcgtctt ctacctggtt ataaccgaac gataagccca taccacggca
     5461 catttcccat ttcttatcac gggaagccac gccgtgtttg tattctttgg aggtgaaatc
     5521 gcaccatagt ttgttccagc ggtcgtccac tacgccctct tgaacgctgt tgtaataatg
     5581 agcaaataga tgtggtaaca tatgctcgcc cacttttggc cagccaatat cattccacaa
     5641 tacatctggt ttatagcggt cgatcagctc taccacttgt ttataggcgt agtcagcgta
     5701 ttcgtaagtc gggcaagcgt ttgagaagtt ttgactttcg gtgaaaatcg gggcagagga
     5761 ataagtccaa tcgataatgc ccgagtagta agcgcccatt tttaagcctt gctcccgcac
     5821 attttcagtc agctcgccca ttaaatcccg cttcggtccg gcttgcatgc agttgaaatc
     5881 ggtatattta cttggataga gacagaaacc atcgtgatgt ttggtggtta ataccacata
     5941 tttggcgcca gctttggcaa acagtttcgc ccattctgct ggttgccatt tttccgcttt
     6001 ccatagtggc aggaagtcgt tataggcaaa ggattcgcca taagttttga tatgatgttc
     6061 gtaggtcggg ccttttttga cattgatgga attaaaatac cattctgcat aagggttatt
     6121 acagaaccac tgttcatcaa tatcaatttc acctaactga caggtcggtg gggcaaaggc
     6181 gggtacggag taaatccccc agtgaataaa aatcccaaac ttagcatctt cataccactc
     6241 tggtacttgg tgctgtttga tcgactccca actggcgtgg aaacgttttg gtgaagtgtt
     6301 gcaactcata cacagactcc tattgaatct aaatagaact aaactgaaat acattttcca
     6361 ttttgataaa aataaaacaa tttgtttcga taaaaagggg ggggtttatg tatgaaatgt
     6421 gaacttcatc acacttattt tgtgataagc agattgagga atgcccgata ttcgcttttt
     6481 taggaaaatt cgggcatttt tacggctagc aagcggtctg tttagccgga ttttttgcaa
     6541 aacaaaaccc cttgcttaat atcataagct aaggggtttt cacttaaagc aatatgatta
     6601 acgttgttgc cagagatcga ttaaagtgcg atatttctcc gataccgcag aaatcaaggt
     6661 attgtcgtcc aattcaccgg caagccattt ttcggacggt tcaccgaaaa tggtgcggcc
     6721 gactgcaaag cctttcactt tctcacagcc ttttgcctgt tcaaaggtgt ctttaaacac
     6781 ctctaacggg gcatctaaac ctaaaattaa aataccacga cagtccggat cgtttgcctc
     6841 aatggtggcg gaaatcgctt gccattgtgc gcgagaaaca tccggcaatt tccaccattc
     6901 cggcttaatg ccaagttggt agaaatgtgc tatcgcttgg ttatagaaag cctcactctg
     6961 tggcatatca gcaggcagaa taatttccaa caatagctcg tgaccggtac ggcaacaggc
     7021 ttgatacacc tcttttaact tgcgatcttg gcgggctttt aaaccgcttt catcattcgg
     7081 gtggtaaaat gccaagcatt tgatgacgtg atcgcgtggg aatgatgcta gctgagagcc
     7141 taaatctcca tactctaatt ccagcgggcg ggaagacggc tcttcaatcg ggcgggcaat
     7201 ccagtaatct ttgccggtaa tctcattaag tacatcttgc ccgaaagtgg tatcggctag
     7261 gatccctgct tgattttgtg gtaagccgga ttgagttgcg gtttgttcca tcgcttttaa
     7321 tagcagctgt ttcagtttcg gtatgcgagc aagatctgca ccgactttat ttgccatatc
     7381 caccaactgt ttgcggtgat caaaagcaaa aatacagaga ttttcccact gttttttgcg
     7441 ggtggtgaca cggtgtaagt ggtttaaacg ctcatctaaa tccggacgtg gtacactctc
     7501 tgcacgggtt aaataatcgt caagttcacg tttggtcggc attgccggtg aacagccgtg
     7561 acgagatacc actaacgctc cgcacgcatt ggcatagtgg cacgcttgtt cccaaccttc
     7621 gttatttaag tagccacgca gtaagcccga cataaaagca tcgcctgcac ccagcacatt
     7681 cagcacttct acacgcacgc cgtacacatt caagccaccg tctaaatcat tcggcagctc
     7741 gccttcaaat actgaacacc ctaatgcccc acgtttgcac actaatacgg cattagagac
     7801 tttacgcaca tttttgagtg cagtgagagt atcggtcgaa ccgcctgcga tatgaaattc
     7861 ttcctctgtg ccgacaatca gatcgaaatg gtgcaatact tcttgtagcg atttggtgac
     7921 ttcggcactg tctatataac gggtttcgcc atcaccgagt gaggttaatc cccataacac
     7981 agggcgataa tcaatatcta acgctcgttt tacctcattt ctacccgcat attcgagtgc
     8041 ggtcagcaca gcttcacggg ttttagggtt tgacaggtgc gtgccggtaa ttgctaagca
     8101 acggctggaa gcaatatagg cttcgctaaa atcgtctttg gtgattgcca tatccgcaca
     8161 gttatcacga tagaaaatca gcggaaaagt ctctttatct ttaatgccga gaataaccag
     8221 tgcagttaag cgttctttat cggtaattaa atggctggtg tccacgccta cgctttgtag
     8281 ctcttcacgt agaaaacgcc ccatatgttc gtcaccgacc cttgccagca ttgaagaacg
     8341 taaaccttgc accgccgtac cgtaagccac attgcctgac gagccgccga ggtattttga
     8401 aaatgtggtc atctcttcca aacgggaacc gatttgttgc ccataaaagt ccaccgccac
     8461 acgccctaaa cagataagat ccaatggacg atcttgatga ttaactgttg ccataaccgc
     8521 tccttacaac aagtaaattt gaatacacac tatacatcat acaaaaatga aatttttatt
     8581 tcaaatttat tctatttaaa ttatcatttc tgtgatctag ctctcacttt gaaaaataag
     8641 gcaataactt cgcagaaatg agaactggat cacatctttt tttcgatttc cttgctatta
     8701 taaatgaaat aaatttttca tttgtaaaaa tcagtagaaa accgaccgct tgcaaaagcg
     8761 atgtaagcca gaggaggctt tatgcagact actcgtttaa cggttgcaca ggcgttagtg
     8821 caatttttgg ataatcaata tttagacgtg gacggcaaag agattaaatt cgttcacggt
     8881 gttgccgcta ttttcggcca cggcaatgtg ctgggaatag gtcaggcgct tgctcaaaat
     8941 gccggtggtt taacgctcta ccaaggcaga aatgagcaag gtatggcaca tttggcgatg
     9001 ggctttgcta agcaaaatct gcgtcaaaga attattgcct gtacttcctc ggtcgggccg
     9061 ggggcggcaa atatggttac ggctgcggca acggcaacag cgaaccgtat tcctcttctt
     9121 ttattaccgg gcgatgtgtt tgcgacccgc cagccggatc cggtactgca acaaatcgaa
     9181 caaccttacg atttaagcat tagcactaac gatgcgttcc gtgccgtcag taaatattgg
     9241 gatagagtgc aacgccctga gcagttaatg agtgcttgta tcaatgcgtt tcgtgtgctg
     9301 accgaccctg ctgaaaccgg tgctgtcacc attgctctgc cgcaagatgt acaagcggag
     9361 agttttgatt tcccgaacag cttcctgcaa aaacgagtgc atcgcttaga gcgtacaccg
     9421 ccgacaacag cgatgattaa gcaagcggta acattaattc aaaccaagaa aaaaccgctg
     9481 attgtctgcg gtggcggtgt tcgctactcg caagcgggtc aaattctgca aaaatttgct
     9541 aaacagttcg gcattccatt tgctgaaacc caagcgggta aaagtgcggt ggtgtccgat
     9601 cacgcattga atgtaggcgg agtgggagaa accggttgtt tagcggcaaa ccgtctggca
     9661 aaagaagcgg atttagtgat tggcattggt acacgctaca ccgacttcac gacttcttcc
     9721 aaatggattt tccaaaaccc aaacgtgcag tttttgaaca ttaatgtggc acgttttgat
     9781 gcgtacaaat tagacggcgt gcaactggtt gccgatgcaa aaactgcatt agaggcgtta
     9841 agccaagcca ttcctactga ttatcaagct caatgggaga atgaaatttc acaggcgaaa
     9901 gcggattttg ctgatgaaat tcaacgcctg cgcgctgtgc aatatcaccc tgatttcacc
     9961 cctgaaatta acgataatct tgatcataaa gcggttcaca atgaatttat tcagctcacc
    10021 ggttcccagc tcacccaaac ccaagtgctg ggtattgtga atgaaaccat tgataacaat
    10081 gcgattattg ttgccgccgc cggaagtttg ccgggtgatt tacaacgcac ttggttaagt
    10141 aaaggtgaag agacttacca ccttgaatat ggctactcct gtatgggcta tgaagtgagt
    10201 gcggcagtcg gcgtgaaaat tgcccgcccc gaacaagagg tctatgcctt gctgggggac
    10261 ggctcttact taatggcaca caccgaatta gtgacctcaa ttcaagaggg caaaaaaatc
    10321 aacgtggtgc tgttcgacaa tatggcaaac ggctgtatca acaacctgca aatcggcaac
    10381 ggtatggaca gtttcggtac ggaattccgc ttccgcaatg gcgaaactaa ccaactcaac
    10441 ggcggtttag tgccgattga ttttgccaaa aacgccgaag catacggctg caaaacctat
    10501 cgggtaacta acgaagccga attgcgtgcc gcccttgatg atgcgaaaaa acagaccgtt
    10561 tctaccttaa tcgatattaa agtgttgccg aaaacaatgg ctcacggcta tggctgctgg
    10621 tggcacgttg gtgtcgcgga agtgtcagag agcgagcgta ttcaacaagc ccgccaacgc
    10681 tccgaagagc agttagctaa ggctcggaag tattagttaa aaaaacaact ccccactttg
    10741 agctacgagg ggagaatatc aagcggtcaa atttgcaaaa tttttgataa aaataaccgc
    10801 ttgtatccaa atttatctct ataaatcaaa cgaggtttct ctatgaaagc tgaaaacatt
    10861 caattaggca ttgccccaat cggttggacg aacgatgacc tgccggaact gggagcggaa
    10921 aacactttcg agcagtgtgt gagtgagatg gcacttgccg gctacacggg ctgtgaagtg
    10981 ggtaataaat atccgcgtga tgttgccgtg ttaaaacaca aactggacgt gcgtggcatt
    11041 caaatctgta atgcgtggtt cagcaccttc tttgtggacg gtaaacggga agagaccatt
    11101 gccgagttca tcaaacatcg tgatttcttg catgcaatgg gggcaaaagt gatcggctgt
    11161 tccgaacaga gccgtagtat tcaaggcaca accaaatctg tgttcaaaga aaaaccgatt
    11221 ttcaacgatg aagattggaa acgtatggca gaaggctata acgaattggc gaaacttgcc
    11281 gccgaaaaag gaatgaaagt ctgcctgcat caccatatgg gaaccggtat tcaaacaccc
    11341 gaagaggtcg atcgctatat ggcagaagtg aatgacgatg tctatctgtt gttcgattca
    11401 gggcatttgt actattccga aggctgccaa aaagcgatgc tcggcgtact ggaaaaatat
    11461 atcgaccgta ttatccacgt tcacttaaaa gatgtgcgtg atgaagtcgt ggcggaagta
    11521 aaagcaaaag atttaagctt tttagaaggc gtgaaaaaag gcacctttac tgtaccgggc
    11581 gatggtgtca ttgatttccg tccgattttc gatattttag aaaaacacga ctacaaaggc
    11641 tggatggttg tagaagccga acaagatccg gcacttgcca acccgtttga atacgccctc
    11701 aaaggcagaa aatatatccg tgaaaccgca ggagtttaat atgttaaaag taggtattat
    11761 tggtgcaggc cgtattggac gtgtgcattc tgaaagtatt agcaaatatg tcaaaggtgc
    11821 tgaaatcaaa gcaatctctg atgtaaaaat taccgaagaa ttgaccgctt gggcaaaatc
    11881 gatgggtatt ccggaagttt atgatgacta taaaaaaatc ctgcaagatc cggaaattga
    11941 tgcggtattg gtctgctctt ccaccaacac tcacgctccg atttcgattg aggcagcaca
    12001 ggctggcaaa cacgttttct gtgaaaaacc ggtagatgca gacccagcta aaatccgtga
    12061 agtattgagt gcagtcgaaa aagccggtgt aaaattccaa gtgggcttta accgccgctt
    12121 cgaccacaac ttcaaggcga ttaaagaccg tgtggcggca ggcgacatcg gcgaaccgca
    12181 cgttatccga gtaacctctc gtgatccgga agccccgcca atcgaatatg tgaaagtctc
    12241 cggcggaatg ttctttgata tgaccattca cgacttcgat atgattcgtt atttatccgg
    12301 cagcgaagtg gaagaagtgt ttgcagtcgg caccgtatta gtaaaccctg aaatcggcaa
    12361 agcgggcgat attgatactg ccgttatcac gttgaaactg aaaaacggag caatcggcgt
    12421 aatcgacaac agtcgcaaag ctgcctacgg ctacgaccaa cgtgcggaag tgttcggctc
    12481 gaaaggggca atccatatca ctaatgatac cggctcaact gccgtattct caggtgaaaa
    12541 cggcgtggtg gcggaaaaac caaaatactt cttcttagaa cgttatatgc aatctttcgc
    12601 cgatgaaatc agctgtttcg tagattctgt ggtaaacgac aaaccaacgt tagtaaacgg
    12661 caatgacggc ttacaaccgg tacttattgc cttagctgcg aaaaaatcac tggaagaaaa
    12721 ccgaccagtg aaattgagtg aagtggcgta attgatcata aatacaagcg gtcggtttta
    12781 ctcgctacac tcgcacttcg tgccatcggc atagccgatg ttcaaacgca agcattttgc
    12841 agaaaaattt gtaaaactga ccgtttgagt ttttatttat tatcttttgg gaaataaata
    12901 taatcgtgct caatttttat tgattgttta aataaataga aggctggaat tatcaaaaga
    12961 gttaatacaa taccaataaa atatttaaaa ttttctgttt gcattccaat atattcaaaa
    13021 gtgaatctaa tcccaagagg gtaattcccc cattcttttg ccaaacagat atgaataatc
    13081 aaaaagaaga taatacctaa aaaacgtcga gatattgata ttctgactct cattttttct
    13141 tctttttcac atagtatctc aactctgcat tattttcctt agctgcttgt atttttaata
    13201 ttgaaagcat ttcgtatttg tgtatatctt caattttagg cagctcataa gtgttacatc
    13261 ctattttatc aagaaggtct gataaaataa aaatatgtaa taaacgcatc attgtaaata
    13321 atgcggtaaa tgtaactgaa aaaaagaaaa ttgctgcaat ataatcaatg gtttttgtga
    13381 gatatatttc cttttgctgt atcgcaatat agataggcac ttgatgaaca agttggaagt
    13441 aagcatccaa ataaacaact atcaatatga agcctaaaaa ccattctgtt tgcataagaa
    13501 cattgatagg gctgtttttt gtgctaaaaa tttctttaat tgaactcata atttttcctt
    13561 aaatgtaaaa aaacgtacct caaccaattt gagatacgtt tctattttaa tgtaattatt
    13621 aaatctcact cacctccacc caccttttct cctccgccga ttttgctatc gcttccaata
    13681 ctctggagat tttatagcct tcttcaaaat cgggatacat cgggtcatca gcggcgatgc
    13741 cgttgatgag gtcacggatt tccaccgctt tttggtcgtt aaaaccgatg ccgtgaccgg
    13801 cactcacaca aaaagcagcg tagtcgggat gttccgggcc gactaaaatg gttttgaatc
    13861 cttggcggga tttatcgtca tcgtgcaaat agagtttgag ttctgccatt cgttcttggg
    13921 tatagctgag cgtgccttta gtacctgtaa cgacataggt cagccccatt ttacgcccgc
    13981 aggaaatgcg ggagctttcg agcgtgccca ttaccccatt agcaaatcgt atgagactac
    14041 ttgcctgatc ttcgttttct actttgattt tttctgctgg attgtgcgga ctagggcgtt
    14101 cttcaatcac tgtttgcata tcgccgataa cacgctcaat gtctgaacca accaaatagt
    14161 gtgcgatact gacaatatgt gcggctaaat ctcccaacgt accaagcccg gctttggctt
    14221 tgtagcagtg ccaatcaatc ggggtattag ggttggcgag ataatcttcg ttatgcgtgc
    14281 cgtagaaatg caccacctca ccaatttcgc cgttttgaat aatttggcga gccagttggg
    14341 tggttggatt tttgatatag ttgaaaccga ccaaggtttt aactcccgct tgttgagcgg
    14401 caatcaccat ttctttggca tcttgggcgg tgagtgcaag cggtttttct gaatagacgt
    14461 gtttgccgtg tttaatcgct tctaaggcaa tttctttgtg cagaaaattt ggtgtacaaa
    14521 tgtccaccac atcgacattc ggatcggtaa ccaattttcg ccaatcgtca gtagcacgct
    14581 gaaagccaaa ttctcgggct ttttgatcgg ctaatgcttg gttgatttcg gcaaggtatt
    14641 ccagttggat tttgccttta agtggaaaca gggttggggc ttgagcgtag gcaatggcgt
    14701 ggcagcgacc gatatagccg gtgccaacta agccgatttt gatggttttc ataatactat
    14761 cccagtaggg tgcgtttgct aacgcaccat tagaattttc agtgcattgg caaacgcacc
    14821 ctataagaaa gcggtctggt tttgcaaaat ttttacaaaa tcagaccgct tgtgttagct
    14881 atctataatg ctcctcgtct gctcttcgca taggtgtcga aggcaacggc aaggatgatg
    14941 attaagccca taatgatttg ttggtagtaa gcggaaacgt ccattagtac taaaccgtta
    15001 atcaatacac ccataataat ggagccgatg atggtgcctg tgactcgtcc gtatcccccc
    15061 attagggaag ttccgccgat aaccactgag gcaattacac gtagctcaaa agaaacgccg
    15121 gcaacggatt cggcactgcc taaacgggcg gagagaatga aaccggcaag tcctgccaat
    15181 gctccgatca ccacataaac actgactaag gtacggttga tattcacacc ggctaaacgg
    15241 gctgcttccg gattgccacc taccgagtac acatagcgtc cccagcgggt tttatgcaat
    15301 gcgaagtaac cgataatggc gactacggca aaaatcacta ccggcaccgg tacgcctaaa
    15361 atatcgccac gtccccacca gcggtaacct tcgtcaaatc cggcaattgg cgagccgtta
    15421 tttaacacaa gtgttaaacc acgccaaatt gtcataccgc caagcgtgat aataaacggt
    15481 ggcagtttga gtttagtgac gcctaagccg tgtaaccaac caatgaaagt gcctaaacct
    15541 aaacagatac tcaaacctat cagccagctc attccgcccc aagcaccggc ttctagcgtt
    15601 accgtattat cgcctttgat aatataggcc gcgaacattg cggtaacagc aaggattgag
    15661 ccgacagaga gatcgatccc tgcagttaag atgacgaaag tcattcctac cgccataatg
    15721 ccgaaaatcg acacttcggt taaaacgtta tagatgttgc gttgcgataa aaatgccccg
    15781 ttttgtgaag tgaagaaaat aatcagtaag atcaagaaaa tcagtacgcc gaaacgttca
    15841 aagaaggcga taaaatcgat ttttcctttg tcatcgacca tacccatatt ttttagaaat
    15901 tgcgttgtca tacaaacctc tcttatgctg ccgctttacg ctgaacgcca acagccatca
    15961 ttgacattaa tttttcttcg gtggcatcat cgccgtgaat ttcgccagtt acccgacctt
    16021 cggaaagagt gataatgcgg tcggaaatcg ccataatttc cggtaaatcg gaggaaatca
    16081 ctaataccgc aacaccttgt tttgccatat caaacagcac ttggtgaacc tcagattttg
    16141 ccccaacatc aatacctcgt gtcggctcat caacaattag tactttcggg ttgagtgcca
    16201 tacaacgggc taaaatcact ttttgctgat taccacccga taatttgcgg gcttcaagtt
    16261 cgctacttgc cattttgatg cgtaacgctt ggcggtaggt ttcgattaaa tcatcttctt
    16321 tttgattatc gacaaacaag ttacccttca ataatttgct taaaatcggc agggtcatat
    16381 tggttttaat cggtaaaccg agaactaagc cctctttttt gcgatcttcc ggcacaaggg
    16441 caaccccttt ttccaaagca tctaacggcg aggtggcagc ataaggctta ccgtctaggc
    16501 tgatttcatc agccgtataa ttttctaccc caaacaagca acgggcaatt tcagttcgcc
    16561 ctgcgccaac taagccggcg atccctaata cttcaccctc gtgaatgtta aaagagacac
    16621 cgtggagagc aatgccgtgt ggatcgaatg ccggtttttc ccgatgcaga tttttcacag
    16681 ctaaacgtat tggtcggtgg gtatggtgag tctcttctgg tggacggcga gtaaaggcaa
    16741 cttcacgtcc taccatcatt ttaataattt gatcgacatt cacatttttg acttcaccct
    16801 caccagaata gcgcccgtct tggaaaacgg tgaaacggtc gcatagggca aacacttcgt
    16861 ttaagcggtg ggtcacataa atcacgctaa tgccacggct ttttaaatca cgcacgattt
    16921 ggtgtagtgt ttccacttct ttatcactta aggcagcgga cggttcgtcc ataataatca
    16981 acttagcgtt taaggtcatt gcacgggcaa tctcgaccat ctgctgttgt gccacactta
    17041 aacgggaaac ttgggtgtca ggtttgatat ttaagccgag attatccagc actaattgtg
    17101 cttcctcatt caccgctttt tcattgacga ataaaccttt caccggctct ctgcccaaaa
    17161 agaaattttc agcaaccgtc atattcggta acaagttaaa ttcttgataa atcgttacga
    17221 tgcctttgag ttggcgatca aacggggaat cgctcaaact taaggtttca ccgttaaaga
    17281 taatttcgcc acttgtttgt ggctgagcgc ctgaaagtgc ttttaataag gtcgattttc
    17341 ctgcaccgtt ttcacccaac aatgcgtgaa cttcgccttt tcccacagtc agtttcacgc
    17401 cattgagtgc cataacacca gagaaacttt ttgtcagatc tttcacttca agtaagggag
    17461 tagacataca acctccgtaa aaaaacgccc tccggcctca gccggaaggc agataaatta
    17521 ttcgctgatt tctgcaaaac gttctgcttc ttgcagatta tctttcagga taatttttgg
    17581 cggatagtcc gcaccgctca cttctttgct ctcacggata ttgccaacca cttgctctaa
    17641 tgcggtgctt accgcaaaac ttgggcgttg gtcagcggtg agatacaacc agccatcttt
    17701 cacacgtgca agagcctcag gggttgagtc aaagccggtt actaaaatat cgccggcttt
    17761 caaaccttgg ctacgcaatg cttcaatcgc accaagtgcc atatcgtcat tcgctgaaat
    17821 aatcacttct ggtttctctt ttaaggttgg taacacactt tcaataatgc gtaatccctc
    17881 agaacggagc cagttaccgg tttgatcaac cacgatttta tatttgtcac caccggcagc
    17941 taattcatca cgaatacctt tggtacgttc aatgttagag gttgaacccg gctggccggt
    18001 taaaagaata atttttgccc cattcgggta tttggctttc actgcttttg cgacttcttg
    18061 accgcctgtg tagttatttg ccccaaagtg tggaaccggc ttactgcttt caacttttcg
    18121 gtctaatgtc gcagcaggaa ttttttcttt gataatttct tccactgcac ctgaaatcgc
    18181 attcacatca ttcggggaaa tgataatgcc tttagcacca cgggtaattg cattttctaa
    18241 gtcagaagcc tgcttagtag aagaactttg cccgtctaat acttgcaatt tgatctccaa
    18301 ctttttactg gtttctaccg ctactttttg tagttgaact tcaaacgggc tggataggtt
    18361 cggtaagcta aataccacaa gctcatcttt cgcaaagctg ttcattgcta agcctaacgt
    18421 taaagcactc aatacaaatt taatacgctt tttcattaca aaatcctctt tttaagatgg
    18481 gaattgacgg cgaatattgc caatgttact tcaaactgcg aaggaaactt aagtcttatt
    18541 tcaataataa acctcaatga aataaatttt tcaatttaga atatatcatt caaaattttc
    18601 caaaccaaat gctttgtttc aaatttgtta cgtagatcac atttttagga agagataaat
    18661 gcaagcggtc aaaacaggtc gcttttttgc aaaaatttga ataaaaatga ccgcttgtga
    18721 gatgaaaacc aataaaaaag cggtgatttc tcaccgctaa acactcaaaa aagaaatgta
    18781 ctcgttatta taaggttggg aaattaaatg ccgcttggct acgcaccgag ctgtccggcc
    18841 aacgggttgt gatggttttc atacgggtgt agaaacgcac gccgtctgtg ccgtacacat
    18901 tgagaggccc gaaaatagag gctttccaac cgccaaagca gtggaacgcc attggcacag
    18961 gaattggaat attcacgccc accataccgg cttgtacatc ttgggtgaat tgacgtgctg
    19021 aatcgccatc tgcggtgaaa attgcactac cattgccgta ttggtgactg ttaatgagtg
    19081 cgatcccttc ggcataatct ttcacacgca caacagagag aaccggtccg aagatttcat
    19141 cttgccagat ggtcatttca ggtgtcacgt tatcaaacag cgtacctccg acaaagtaac
    19201 cgttttcaaa cccttcgact ttatagcctc gaccatcgac acagagtttc gccccctgtg
    19261 ccaccccttg atcgatatag ctctcaattt tggctttatg ctctttggaa atcaccggcc
    19321 ccatatcatt ttcaagcacg ccttgtggta gtaagcctga accgattttc aatgctttga
    19381 ccttaggaat tagctcttca atgattttat cggctaattc atcaccaaca actaccgcta
    19441 ccgatagtgc catacaacgt tcaccggctg caccaaatgc tgcgcccagt aaggcattaa
    19501 ttgtgccttg tacatcagca tccggcataa tcaaagcgtg gtttttcgca ccgcccaagg
    19561 cttgaacccg tttgccgttt gctgagcctt tctcataaat atattgtgca attgcagttg
    19621 agccgacaaa actcaccgcc tgaatgcgag agtcggttaa aagaatatct accgcttctt
    19681 tatcaccttg caccacattg aatacaccgt ccggcaagcc ggcttctttt aataattctg
    19741 ccaagcgaat agacacactt gggtcttttt cagacggttt taaaacgaaa gtgttaccgc
    19801 aagcaagtgc cactgggaac atccacatcg gtaccattgc cgggaaattg aacggggtaa
    19861 tgcctgccac tacgcccaac ggttgctgga ttgagtgaat atcaatgcca cgccctgcat
    19921 tggcagagaa ctcacctttt tgtaagtgtg gaataccggt ggcaaattcc accacttcta
    19981 aaccacgggt taattcgcca atcgcatcgg aatagatttt gccgtgttct gagctgatta
    20041 aacgagccaa ctcatcaaag tttgcttcca ataactcttt gaatttaaat aaaattctgg
    20101 cacggcgtag tggtgattgt tgcgaccagc ttttgaaagc ctgatcagcc actgcaatcg
    20161 ctgcatcgac ttcgtttggc aggcttaatt tgactttttt ggtctcttca ccggttgcgg
    20221 gattaaatac cgaggtaaca cgctggcttt ggctttcaac gactgcaccg ttaataaaat
    20281 tttggacaaa ttggctcatt ggaggctcct tatcaactga ttgagaggaa aaacggataa
    20341 aatacagccg ataaactacc gctaaaaatg gaaaatatca atacacaata ctaaaaaatg
    20401 aaaaatttat ttcatttttg tgatctaagt agcaagttta cagattgcct gtattaaaat
    20461 gcgataaaat ttcgtatagt ttgaagacgt attccatttc gttaagcgag gtttcctatg
    20521 tctagtccat tattgtctaa acccaatttc agccaacctg agggattaat tcaacacatt
    20581 acccctgaaa atgcacagtg ggaatatgtt ggatttgatg tttatcagct cagtaagcaa
    20641 caaattacgc tggaaagtgg tgaaaatgaa ctttgtctag tctttgtgac cggttttggt
    20701 tctgtctatg ttgatgatta tacctttgaa aatatcggca accgtaccac accatttgaa
    20761 aaaattccgc cttatgcggt ctatattccg ccacacaaaa gcgtgttgat taaaactgaa
    20821 ctcaatttag agttagcagt gtgcaaagca ccaagtcaag gtcactatcc ggtgcgtttg
    20881 attagcccga accatatcgg tgtggaaaaa cggggctttg gcaacaatca acgcttggtg
    20941 cataatattc tgccggaaac cgaaccagcc gacagtttgc tggtggtgga agtctttacc
    21001 gatgaaggca ataccagttc ctacccaagc cacaagcacg atgacaaaca ctctaccgaa
    21061 gaaacctatt tggaagagac ctactaccac cgcttcgatc caccacaagg ttttgcaatg
    21121 caacgggttt ataccgatga tcgcacactt gatgaatgta tggcggtgta tgataaaaat
    21181 gtggttaaag tgcctcgtgg ctaccaccct gttgctacca ttgcaggcta taataattat
    21241 tacctaaatg ttatggcagg gccggtacgc aagtggaaat ttacttggga acaagatcat
    21301 ctttgggtga acagtgaaac ctataaagcc aaatttgagt aatacaagcg gtctttttcc
    21361 accaattttt tacaaaataa gggattaaaa atgggacgct tttggcgtcc cattcggtta
    21421 tactttcata caaagatatt ttatttctaa aaactcttca ataccgtatt tcgaaccttc
    21481 ccgcccaagc ccggactctt tccagccgcc gaatggggct atttcgttac tgatgatccc
    21541 ctcgttaatc ccgacaatac cgtattccaa ctgttccgac actcgccaaa ttcgcccgat
    21601 attttggctg aataagtaag ccgccaaacc aaactcagta tcattcgccc actggataac
    21661 ctcttcttcg gtttcaaatt tgaaaatcgg ggcaagggga gcaaaggttt cctcacgggc
    21721 aactttcatt tcttgagtca cattttttag aatagttggt tggaagaatg tgccgcccag
    21781 tgcgtggcgt tcaccgccta acaccagttc tgccccaagt gaaagggcat cgttaatgtg
    21841 gctttcgact ttttccaccg ctcgttcact aattagtgca cccatcgtga cctcttcctc
    21901 aaatggcgag ccaagcttga tttgagccac tttctcactc aattttgcaa gaaattgctc
    21961 ataaatgccc gcttgcacat aaattcggtt agcacagacg caggtttgcc caccgttacg
    22021 gaatttagag gcaaacacgc cctcaaccgc tgtatctaga tccgcatcat caaacacaat
    22081 cgccggtgca ttaccgccta gctccaatgc caattttttt accgtccctg ccgattgttg
    22141 cattaaaatt ttgccgacac gggtggagcc ggtaaaggtg agtttgcgta cggtcggatg
    22201 agtgctcaac tcccgcccta taccttcggc atcgctactg gtaataatgt taaatacccc
    22261 tttgggaata cctgcttgat ccgctaattg ggctaatgct aaggcagaaa gcggggtttc
    22321 tgccgccggt ttaatcacaa tcgcacaacc tgccgctaac gccggtgccg ctttacgggt
    22381 aatcattgca tttgggaaat tccacggtgt aattgctgcc accacgccaa tgggttgttt
    22441 agtcacaaac aagcggcgac cggctttatc ttgtggcaag acatcgccat aaatccgctt
    22501 gctttcttcg gcaaaccact cgataaaact ggctccatag gcaatctcgc ctcgactttc
    22561 tgccaatggt ttaccctgtt ctagacttaa aatctgggcg agttcttctt ggttttgcat
    22621 aatcagctca aaccatttac gcaaaatcac ggaacgctct ttgggcaata atgcctgcca
    22681 ttttttctgg gcaactgcgg ctgcttcaat cgcttgagcg gtttcttccg tgcctaaatc
    22741 agccacctca ccgattttct caccattagt cggattctgt accgtaaagg ttttcccatt
    22801 ttggcttgaa acaaatttac cgttaatata ggcttggaaa cggagtagca aaaatttgtg
    22861 atttggcata atcactcctt gaatggttaa aaagagaaca atgttgacta taatccaatt
    22921 tcagtaaaga gaaaagagct gaaaaataac tttttactca ataataaagc accttagaca
    22981 agcgttcttc taaggtgcag ccatttatta ttgagaatta acttaaattc tctacatatt
    23041 taataattaa gatacaatca ttggagccca tacgaatcca aaagcaagcc caacagcaac
    23101 cccaactaaa gctggaagca taaacggatg atctataata tatttgccag cacgagttga
    23161 acccgtagta tctaattcca tcgctgctaa tgaagttggg taggttggca ggataaaaat
    23221 gcctgtaatt gcaatgaaag aagccaaaat tgcccagtta gggatcccca agcttactgc
    23281 taatggaata ataagagcag aagttgaccc ttgtgaataa agtaatgcac aagtaaaaaa
    23341 gagtactaca gctaacaaca ttggatattc agttaaaata tttccaatag aggctttaat
    23401 ttctattaaa tgtgcatcga caaaagtcat acctaatgta acaatcccga taattaccgc
    23461 aatagaagtc attcctgagc ggaaaattgg actagccaca atcgcatcag gtttaatttt
    23521 tcctaccaac atcattaagc acgaagaggc taacatcatc acaataatta tatctcgggt
    23581 actcattgac ttcccatttt catatactgg ctttaaccct ggaacggctg caagtagaac
    23641 aaccaataca gttgctagta agaaaatgcc aatcaatagt tttgctcctt ttgaataggt
    23701 agattcactc gctaacttac gagatgggct gacgattcct gcttttaaac gttctaaata
    23761 aacaacatca ttttgcaatt cacagccctg cttagaagca acaaatgcaa caaccataga
    23821 ggcaataaaa gaaacaggta agcatactgc tattacttga aaaaatgtaa cactgttgtt
    23881 ttccattact gctatcatag ccgccattgc agcactcaat ggagaacctg caatagctaa
    23941 ttgagaagac acaaccgagc cggctaatgc tcttgaaggg cgaataccac tctcttttgc
    24001 cacttctgca atcacaggta aggtagaaaa gacgataaaa cctgtaccag ccataatcgt
    24061 cattaaccaa gtaatcatcg gtgcaataaa gttaatatat ttaggatttt tacgcatcaa
    24121 tacttccgca taacgaacaa gtaaatccat acctcctact gcttgtaaca cagcagcacc
    24181 tattacaaca gtcataataa tcagcattac atcaactgga atagaaccca taggtaaacc
    24241 aaaacctaga gttagaatcg caagccctaa accaccatat agtcctattc ctaagccccc
    24301 agaacgcagt cctaaataga ttgcagcaag taccacaaag atttctgcat aaatcatatc
    24361 catctcctta aattaatgag tttgatacca atcaattgct tttttaacca ataccaacgt
    24421 tgaatctaca taacgcattg gctcacgctc tatttcttga gctaaaataa taggtacttc
    24481 tgtacaacct aaaataatta cttctgcacc taaacgaatg agttcatcac gttgttgtaa
    24541 cattagctct gtggctttct ctaactcacc tgattttaat aaatagatac tatccattac
    24601 tgccttttga ccattttctg tcgggcaaat aaaagtcagt ccttgtgctt caagctgttt
    24661 tttgtatagt tctaccgcca aagttgcatc agttgccaac aatccaaccc gccgttttcc
    24721 acacttcaaa acctcatcaa ccacacaatc aatcatactt agcatctcta cctgactttt
    24781 ggccttcaag tgttcaaacc aataatgtgc agtattacat gcaataatga tgcaggttgc
    24841 ccctgcactc tcaagccctt caatatattt ttccattacc ggagccggat tctctccatt
    24901 tgctaaaata tagcgagttc tatctggtat gtcgggaata gaggaaatca ataatggaat
    24961 atgagcttga tcacaatcag ctggtgttaa gcggataaat ttttggaaca tatctgctgt
    25021 tgctgcaggt cccattccac ctaaaatgcc aataatattt cttgccatac acttctcctt
    25081 ataagattta atttactgcg atgacttata caaacaagtt atgcaaaaaa gaaatgcaat
    25141 aaatcgaatc agtatgcaaa aaatgcaaca aagctcttta tagatagaaa aaaaatcttt
    25201 aaaatcaata aaatactaat atatcaaata tagatttaat gcatttaatg aaatcataat
    25261 gcgaaatttg cattggttag gatatgaaaa acatagaaac aaaatggtta gaagatttct
    25321 taacgctaga agaatgtcgc catttttctc aggcagcaga aaaaagaaat ttgtcacaat
    25381 ctgcatttag cagacgtatt ttagctttag aagaagttgt gggagtgaag ttatttgatc
    25441 gtacctctat tccattacaa ctcacagaac aaggtaagct atttcattca caaacgcgaa
    25501 acttattaca acaattacag aataatctag atgaattatt aggacataat tgtaacttac
    25561 ctaatattaa atttgcggtg gctcattccc tctctctttc tattatgccc aaattaatta
    25621 agaaattatc acaaacaaat gaaaatttta tttattccgt tgaagcaatc gatgttgacc
    25681 aaacagtaaa tactctaatt gaagggaaaa gcgattttat tttttcattt tatgatgaaa
    25741 aattaatgca accacccttt atgtctttag aaataatgca atctaaactt taccctattt
    25801 ccccaataga cattacagga tctgcattat ttagtttaaa cgataaaaac ataccattat
    25861 taaattatac accaaattcc tatatgggac gcttagtgaa tagaaagtta gctaatacga
    25921 ttcaattaaa aacaaaattt atttcatcaa tgtcttaatt attaaaaaat atggtactaa
    25981 atcatcaagg cattgcatgg ctccctgaat
//
LOCUS       DANJWC010000015        25291 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.015, whole genome shotgun sequence.
ACCESSION   DANJWC010000015 DANJWC010000000
VERSION     DANJWC010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 25291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25291
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.015"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(435..1177)
                     /locus_tag="PWX02_000445"
                     /pseudo
     CDS             complement(435..1177)
                     /locus_tag="PWX02_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248553.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1348..1686)
                     /locus_tag="PWX02_000446"
     CDS             complement(1348..1686)
                     /locus_tag="PWX02_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL1728491.1"
                     /translation="MKLITTIIQPSKLDQVQDALSEIGIQGMTITEVKGFGRQYGHTE
                     QELSQMSRIDFRPKIKVEILVSNYQTDEVIDAILNASYSGQVGDGKIYVANIEQVIRT
                     RTGETDDEAV"
     gene            1944..3842
                     /gene="dnaK"
                     /locus_tag="PWX02_000447"
     CDS             1944..3842
                     /gene="dnaK"
                     /locus_tag="PWX02_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDL1728492.1"
                     /translation="MGKIIGIDLGTTNSCVAVMDGDKPRVIENAEGARTTPSIIAYTD
                     KETLVGQPAKRQAITNPKNTLFAIKRLIGRRFTDAEVQRDIEIMPFEISKADNGDAWV
                     TVKGEKLAPPQISAEILKKMKKTAEDFLGEPVTEAVITVPAYFNDAQRQATKDAGRIA
                     GLDVKRIINEPTAAALAYGLDSKKENQTIAVYDLGGGTFDISIIEIDNFDGEQTFEVR
                     ATNGDTHLGGEDFDNRVINYLVEEFKKEQAVDLRNDPMAMQRVKEAAEKAKIELSSAQ
                     ETEVNLPYITADASGPKHLNIKVTRAKLESLVEDLVNRSLEPLKTALSDAGLSVSDIN
                     DVILVGGQTRMPLVQKKVADFFGKEPRKDVNPDEAVAIGAAVQGGVLSGSVTDVLLLD
                     VTPLSLGIETMGGVMTTLIEKNTTIPTKKSQVFSTAEDNQSAVTIHVLQGERKRAADN
                     KSLGQFNLEGINPAPRGMPQIEVTFDIDANGIINVSAKDKNTGKEQQIKIQASSGLSD
                     EEVEQMVRDAEANAEADKKFEELVQTRNQADGIAHATRKQITEAGDALNADDKAKIDA
                     AIAELETAAKGEDKAEIEAKIEALIKASEPLMQAAQAKTQGGEQPQQSQKDDGVVDAE
                     FEEVKDNK"
     gene            3984..4286
                     /locus_tag="PWX02_000448"
     CDS             3984..4286
                     /locus_tag="PWX02_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728493.1"
                     /translation="MLKLYPTTENVQPPFYKIYDHKSGETVIATEMENSNTPILMDDF
                     VWFEQIPTTEALLVDTWSVIENRAKQGLLKRAYTKDFEKHLADGKVEIYIAVNPHC"
     gene            4452..5591
                     /gene="dnaJ"
                     /locus_tag="PWX02_000449"
     CDS             4452..5591
                     /gene="dnaJ"
                     /locus_tag="PWX02_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDL1728494.1"
                     /translation="MAKKDYYEVLGLSKGASEKDIKRAYKRLAAKHHPDKNQGSKESE
                     EKFKEITEAYDVLTDSEKKAMYDQYGHAAFEQGGAGAGGFGGFGGGGFGGFEDIFSEM
                     FGGGFGGGGRRQRVVRGDDLRYDIEITLEEAVKGCKKDIRIQTLAECDTCHGTGAEAG
                     SKVETCSHCHGSGRVRRQQGFFVTEAVCPSCHGTGKRIEKPCRSCHGDGRVQKAKNLS
                     VTIPAGVDTGNQLRLSREGAAGENGAPAGDLYVVIHVKEHDIFERDGSNLYCEVPISF
                     TLAALGGEIEVPTLDGKLKLKIPAETQTGKLFRVRGKGVTSPRGGYAGDLICKVVVET
                     PVNLTEEQKALLRQFEESLEGQGKHRPKHEGFFDGVKKFFDNLGK"
     gene            5879..7387
                     /locus_tag="PWX02_000450"
     CDS             5879..7387
                     /locus_tag="PWX02_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HDL1728495.1"
                     /translation="MSKCPVTHLTMNNGAPVVDNQNSLTAGPRGPLLAQDLWLNEKLA
                     NFVREVIPERRMHAKGSGAFGTFTVTNDITKYTRAAIFSQVGKKTEMFARFTTVAGER
                     GAADAERDIRGFALKFYTEEGNWDMVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNMRS
                     ATNNWDFWTLLPEAFHQVTIVMSERGIPASYRHMHGYGSHTYSFINAANERFWVKFHF
                     HTQQGIKNLTNEEAAAIIANDRESHQRDLYEAIERGDFPKWKMFVQIMPETDADKVPY
                     HPFDLTKVWPKSDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGISVSPDRMLQ
                     ARLFNYADAQRYRLGVNHHQIPVNAPRCPVHSNARDGQGRVDGNYGSTIHYEPNSFGK
                     WQEQAQYAEPPLKINGDAAHWNYREDDADYFSQPRVLFNMMNDEQKQALFNNTAAGMG
                     DALDFIKYRHIRNCYACDPAYGEGVAKALGMTVEDAIAARATDPALGQPGLL"
     gene            complement(7450..7671)
                     /locus_tag="PWX02_000451"
     CDS             complement(7450..7671)
                     /locus_tag="PWX02_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDL1728496.1"
                     /translation="MNVRLPASGLVCPFPLVEAKAAMAKLNKGDSLTIEFDCTQATEA
                     IPNWAEEEGYEVTDYQQLGDALWEITVVK"
     gene            complement(7693..8127)
                     /locus_tag="PWX02_000452"
     CDS             complement(7693..8127)
                     /locus_tag="PWX02_000452"
                     /inference="COORDINATES: protein motif:HMM:NF016064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HDL1728497.1"
                     /translation="MTAVLVGLIALLAWPLSSAAGRNFGLGITTPSANLVQYFVTGEV
                     KFINWAVFLVLGILIGSFVAAKFSNEFRFRVPDATTMLRSAGGGALMGIGASLAGGCS
                     IGNGLVETAFFSWQGWLSLPIMILGTFVGAYFTIIRPQRLKG"
     gene            complement(8124..8720)
                     /locus_tag="PWX02_000453"
     CDS             complement(8124..8720)
                     /locus_tag="PWX02_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HDL1728498.1"
                     /translation="MLITGLLCGFLLGFVMQRGRFCITGAFRDLYVTKNSRMFVAFLI
                     AITVQSIGIWLLYEAGSFSSPAEDLPLLAVIIGAFLFGIGIVYAGGCATGTWYRAGEG
                     LIGSWVALIVYGLFSASMRTGVLAPLNQELKSNVIEHRTIYETFGISPWVLVFILSVI
                     TFALTFYHLRKPRGKTITLKPRKTGLAHILFEKRWHPF"
     gene            complement(8829..10163)
                     /locus_tag="PWX02_000454"
     CDS             complement(8829..10163)
                     /locus_tag="PWX02_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDL1728499.1"
                     /translation="MQLYSYICLLFGLAMFISFFTRKLNENIQTTIAITASALVGSLL
                     ILTFGSLGWFHVDKLAISIFEQLDFKSFLLNGMLGFLLFAGSLGIKLPLMKEQRREIA
                     VYALLSTLISTFLIGALIYGVAKLCGLEIGFVYCLLFGSLISPTDPIAVLAIIKSLKA
                     PKRLSMHVEGESLFNDGIGLVIFTTIFAVAFGGQAPTLSGITGLFLQEAVGGIVFGLL
                     TGFVAHKFISATDDGSLEILITLTIPTVGFVIANYLHVSGALTMVVAGIMIGNWTRRS
                     GFSEQSQKYLDHFWEMIDHSLNYLLFLLIGLSTLLVDFTFIGGVLMLAAIPICLLARY
                     ISLWIPYQVLKRFRTYNPYTLRIMTWGGLRGGLALAMALSVPAGAAMVSSSEGELDVR
                     DLILLMTYAVVTFSILVQGSTVEPMIKKSKLVDPRRVALNKLGGPDDLIPHI"
     gene            complement(10274..11422)
                     /gene="dapE"
                     /locus_tag="PWX02_000455"
     CDS             complement(10274..11422)
                     /gene="dapE"
                     /locus_tag="PWX02_000455"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HDL1728500.1"
                     /translation="MKNEIISLAQNLIRRDSISPQDKGCQQEIAQRLEKRGFNIEWLP
                     FGDTLNLWATHGSENGKTEPCIVFAGHTDVVPVGDESQWAYPPFSAEIVDGMLYGRGA
                     ADMKGSLSALVVAAETFVQHHPNHKGKVALLITSDEEAAAKDGTVKVVETLMARNEAV
                     HYAVVGEPSSSKVFGDVIKNGRRGSITADLYIEGIQGHVAYPHLAENPVHTSLGFLTD
                     LTTYQWDNGNEFFPPTSLQIANIKAGTGSNNVIPGELYIQFNLRYCTEVTDEIIKAKV
                     TEMLEKQGLKYRISWNLSGKPFLAGNGKLVEAALQAVENVAKITPRLDTGGGTSDGRF
                     IALMGAEVVEFGPLNTTIHKVNECVSVDDLANCGEIYYQILEKLLANA"
     gene            complement(11552..11899)
                     /locus_tag="PWX02_000456"
     CDS             complement(11552..11899)
                     /locus_tag="PWX02_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HDL1728501.1"
                     /translation="MTTLVYGIKNCDTVKKALKWLEDHNQNPQLHDYRVDGLNADWLV
                     EMEAKFGWENLVNKRSTTWRNLDEEIKNNLNRESALKVLTEQPTLIKRPIVIKEDIAL
                     IGFNEKEYVAAFN"
     gene            complement(11899..12756)
                     /gene="folP"
                     /locus_tag="PWX02_000457"
     CDS             complement(11899..12756)
                     /gene="folP"
                     /locus_tag="PWX02_000457"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDL1728502.1"
                     /translation="MQIHFKNFQKNDRLLDLTTPQIMGILNFTPDSFSDSGKFFSLDK
                     ALFQAEKMLADGASIIDIGGESTRPNAAIVSLEQELERVVPVVEAIRSRFDCLISVDS
                     SKAEVFKESAKVGMDIINDIRALTEPNALAAAVELGLPVCLMHMQGNPQTMQQNPHYD
                     DVLEDVADFLNQRIFECITAGIPKEHIILDPGFGFGKSVKHNYQLLKHLKAFVDSGFP
                     VLAGLSRKSMIGAVLDKPVEQRLLGSVVGALLAAQNGAKILRVHDVAETADALKIWQA
                     MLESDNEEK"
     gene            12745..12882
                     /locus_tag="PWX02_000458"
     CDS             12745..12882
                     /locus_tag="PWX02_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728503.1"
                     /translation="MDLHIFAYILLVQASTLVPLYVDLQHKRGRLRYLTLSLRYLVIS
                     P"
     gene            complement(12865..13827)
                     /gene="rluC"
                     /locus_tag="PWX02_000459"
     CDS             complement(12865..13827)
                     /gene="rluC"
                     /locus_tag="PWX02_000459"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HDL1728504.1"
                     /translation="MTTEKVISAGVQFFTISEDEAGQRLDNFLLAKLKGVPKSLIYRI
                     VRKGEVRVNKGRIKPEYKLQPDDLVRVPPVRVAEKNEAPISTKLNKVAELESQILYED
                     DSMLVINKPSGIAVHGGSGLSFGVIEALRALRPQARFLELVHRIDRDTSGILLVAKKR
                     SALRSLHEQLREKVVQKDYLALVRGQWQAHTKVIKAPLLKNELASGERIVRVSEEGKP
                     SETRFSIEERYANATLVKASPITGRTHQIRVHTQYAGHPIALDDKYGDVEFDSKMKEV
                     GLQRLFLHAHAIRFEHPKTGEEMVITAPLDKTLKGVLAKLRANY"
     gene            13936..14839
                     /locus_tag="PWX02_000460"
                     /pseudo
     CDS             13936..14839
                     /locus_tag="PWX02_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
     gene            14937..15434
                     /gene="rraA"
                     /locus_tag="PWX02_000461"
     CDS             14937..15434
                     /gene="rraA"
                     /locus_tag="PWX02_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005717542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HDL1728505.1"
                     /translation="MRIDTSALCDLYSDQVDVVEPIFSSFGGASSFFGKITTVKCFES
                     NGLIADVLEEEGEGRVLLVDGGGAVRRALIDAELAQLAVDNGWEGIIVYGAVRQLDIL
                     ERLDIGIHALAPIPVGADNTDIGEVDTPVNFGGVTFFPEDYIYADLTGIILSQELLDL
                     SDISE"
     gene            complement(15482..16843)
                     /locus_tag="PWX02_000462"
     CDS             complement(15482..16843)
                     /locus_tag="PWX02_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDL1728506.1"
                     /translation="MLFLNKIKQLFIFAFTLVLSACSLVVYKPVETIKQVNVERGYRQ
                     KNVIAESAKDGNIVVIMFSGGGTRAAALGYGVLEEFKKVRVKETNKGNTLLENIDIAF
                     GVSGGSILATYFALEGKDIIPKFADRFLKANFQKDIITEVFSLSNLPRLSSPQYGRGD
                     LLQERLNFALYHGKKFGDLVKNRKGPFAVISATDMNIGQKLTFTQETFDGLCLNLNDL
                     EISRAVAASSSVPLIFAPLTLNNNGGNCHFSMPQEFSAREKAIDGLITKNIEETNHIL
                     SLYQDSKERPFIHLVDGGLTDNLGLASVLEISDVMGIEEMYLYIHQLGVKNIVVINVN
                     AQNEVSSEIDKTANVPGVADVVNTIVNVPIDRTTQINLQRYRQFTDGWNEYANKQPSK
                     IEMHFVSLGLKELPDGDLKRDVLNIATSFYLPEDDVDKLREAARVLLQQSQEYNAALK
                     ILQ"
     gene            17035..17586
                     /locus_tag="PWX02_000463"
     CDS             17035..17586
                     /locus_tag="PWX02_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL1728507.1"
                     /translation="MAKQIAVLIGSGSKTSFSHLTVRHLQKMAPASIQLNIVDIADLP
                     LYDRDLDENSPAQYTRVREAVAVADGVLLVTPEHNASFSAMLKNAIDVVSRPMGQSKW
                     IGKPAGIVTVGASPAGGVRAADQVRTITSGSFINMPTLPFALNVGNLFAGVFDEQGEI
                     ASEALKSAMQNFINAYAQFVEKY"
     gene            complement(17656..18404)
                     /locus_tag="PWX02_000464"
                     /pseudo
     CDS             complement(17656..18404)
                     /locus_tag="PWX02_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248535.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            18495..19559
                     /locus_tag="PWX02_000465"
     CDS             18495..19559
                     /locus_tag="PWX02_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HDL1728508.1"
                     /translation="MAKFRKLFTPFNIKNLALKNRVVMPPMCQYSATNGVPNDWHFVH
                     YTSRAVGGVGLSIVEMTNVAPNGRITPNCLGLWNDEQQQAFKKLVDSVHAQGAKIGVQ
                     IAHAGRKTQDEPDAVAPSAIHYGELDYAGQNLITPRELTTDEVKALVQAFKDSVKRAV
                     EAGFDTIEIHGAHGYLIHQFYSPKSNQRSDEYGQDKMLFGEQVIQAAREVMPSEMPLI
                     VRISAQEYGENGFGLDYGLQVAKRFADAGADVLDISGGGDGVLHAGNHPEFYAGYQVD
                     FARQVKLATGKPVIAVGMLDNPAVGDHILGMGDADLVAVGRALLRDPYWVLNAQYDQN
                     AADSSEMQFVPKQYERGLFM"
     gene            19744..20403
                     /locus_tag="PWX02_000466"
     CDS             19744..20403
                     /locus_tag="PWX02_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL1728509.1"
                     /translation="MQFFNKEDVINAFHYRASTRSYDGSRKIPAEDFNYILELGRLSP
                     SSVGSEPWQFLVLQNPELRQKIKPYCWGIPTMETSSHIVVILAKKNARYDSQYFSEIM
                     DRRGLQGEAREKAMAVYKKFQEKDIGILDSERSLFDWASKQTYIALGNMMTGAALIGV
                     DSCPIEGFAYQEVNDILAAEGLFDPSEWDVSVMCTFGYRDKEIRQKARKDFETVVKFI
                     E"
     gene            20539..21456
                     /locus_tag="PWX02_000467"
     CDS             20539..21456
                     /locus_tag="PWX02_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL1728510.1"
                     /translation="MTFIIGILAGVLIAFQSAINARLRMHVGAAFLTSFVSFSVGLLF
                     LLGLSLIMQQTPAFSLDNLGNVPLWAWTGGLLGMVGLTANVLIFPKLGGVQTAVMPIL
                     GQVLMGVLIDTFAWLNSPYIAFTASRLFGIGLVLFGVWIAVVLPHLSGLKRKENANLW
                     LWRAVGILGGTTLATQAAVNGEFGRQLGSSLGAATVSFGVGAIGLLLVVLFYEKSLPK
                     LCQPTEGKPFWIWSGSILGSLFILAGVWLVPQIGTGSVVMLVLSGLICGSLLVDKFGL
                     FGITQKPILPLQIIGVTLLLAGVAFIRLL"
     gene            21670..22548
                     /locus_tag="PWX02_000468"
     CDS             21670..22548
                     /locus_tag="PWX02_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL1728511.1"
                     /translation="MSKQIQFTKTGSPDVLQIVDVQIPAPKAFEVQIQIQAIGLNRAE
                     MMYREGAYVIDPVFPATLGYEGAGVVVAIGEGVNELAIGDKVSIIPSFMFTEYGTYGE
                     IVNMPKHAVVKHPDNLSMEQASASWMAFVTAYGGLIEFDKVQKGDVLLEKGADFVIAT
                     SEDDVTAKLFEITNGKGVNVVFDPVGGKEAAKIINAMAQDGRYIIYGALSHDDIAVPV
                     FPILGKHLTVRGYELFEITTVPEKLAQAKTFVYDGLASGQLRPEIDKIFAFDEMVKAH
                     EYMASNQQIGKIVVKV"
     gene            complement(22614..23204)
                     /locus_tag="PWX02_000469"
     CDS             complement(22614..23204)
                     /locus_tag="PWX02_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL1728512.1"
                     /translation="MNDLRVIKTHLAIQNALIELLDEKPFEKVQVQEIIEKALINRTT
                     FYRYYSGKSDLVGKLIKEVKAEYQDLLKKRFESDDLLRFMQTIGNRLLEKRRLILALW
                     QIKTPRHHLYDDMHTLLKNAFIAHAKRITSDDKNWADKNWAEKNWAEKNWDYQAHIFA
                     TIALESHKYYFEQGKLLPIPQVFEAWIEMVQVLKSV"
     gene            23335..24177
                     /locus_tag="PWX02_000470"
     CDS             23335..24177
                     /locus_tag="PWX02_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HDL1728513.1"
                     /translation="MIKVHCLTTGWVQIKIHHQVARFFAHPARVLDVLTDKKWSPKLP
                     IGCWLIEHDDGLILVDTGESSSANDKGYQPWWHPFMQFCERRGVKKHEEVGEILKANG
                     FDPLRIDTVVMTHMHGDHAGGLPNFPNSRFVMSETEKQAINAPNAVMNGYLTMHYPDW
                     FNPCGIAFNDGAFESFEQSHKLTKDGKIRLVPTAGHTLGHLAVVVDMGEYDILIGGDA
                     SYSEQDMLAGNIDGVCVDGHLHQQSTAKMRQLCQRKPTITQFAHDEKSEYRLKNKVFT
                     VVKN"
     gene            24360..>25291
                     /locus_tag="PWX02_000471"
     CDS             24360..>25291
                     /locus_tag="PWX02_000471"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03571 family LLM class oxidoreductase"
                     /protein_id="HDL1728514.1"
                     /translation="MNTLSPIFSQVFGQNQLTLGLIAPFMGYQNHIPDNLTTIADNAR
                     LAENLGFSALWVRDVPFYDPHFGDVGQGLDPMVTLGFLSAHTQNIALGTAGLIAPLRS
                     PIHIAKMATSVDSLSHGRFLLGLSSGDRPVEYPAFGADFASRSERFRESIDLINTLTE
                     QDFPHFKGKHYADLTDNLDLLPKPKHRLPIVAIGRARQEMDWLANVPDAWIWHGVNPA
                     DTANIINTLADLRQKDTPTAFGYAQFVEVLDDKNAKAQLFNNIYLRGGAKGLADFWAK
                     EQEKGVKHLALNLKPTQRPADEVLTELAEYALTQ"
BASE COUNT         7535 a         5460 c         5268 g         7028 t
ORIGIN      
        1 ctacctattt ttaattaacc aatatgcaac aaagccatat taataataag gttatcactg
       61 gtttttatcc cttatttttt atgaaaaatc ttataatatt gaatatttac tccagtttta
      121 atcgagaatc atgttctttt ttattttgtt attctcttta ggtataaaaa aacccggtaa
      181 ataccgggct tttatttcaa tgagttatcc aacttgctag agcgattacc gcaataaaaa
      241 taaataacca aataagttgt aattttacgc ttttgcggtt gatctcctgg ttgtgtaatc
      301 ggcgctgttc taacttttgc cgatatatca ctaaatcggc ttctttaaac gagaagccac
      361 aatgtatgca atgttctaaa ctgtcgctaa tacgctttcg acactctgga caacggtgta
      421 atgccataat tacctcaatt tattactttg cataatcggc tatccgtaca ttgttcaata
      481 tgaacatgat agtattgatg agtatcacga ttgaaaaaca agaaatattg attacctctt
      541 atttttgttc ccataatgta gccgttatca ctgttggtgg ataatatgta gtatttttga
      601 ttctctattt cgatgtaatc ataggtagtt cttagtccgg cacgaaataa ccaatatgca
      661 atgagagcaa actgaaatag agaaaaataa agatgttaaa cagagcaaaa cttcctccta
      721 atagcatctt tctaaagtct agatcaaact tggtattatt ccatctctta cggaagaaga
      781 atacactcac gccacttaca aaaatataga taaatgagat ataaataggc actataccgg
      841 taaataagta aaaagtgaga ataaccggta ctgtaaatac agaaaccaac acaatcaccc
      901 ttaaccaacc tagatttcgg aaataatgca gctgaaaaac tttatttagt aagctataac
      961 ctagcgcata acacaagaat aagataactg aagtaccgaa tacataagcc aaacttcttg
     1021 ccatactggc attgccgatt tcaacatgcc accaagggta gccatgaaat atggcttgtc
     1081 cccaaccgta acagtaagcg gtagcaaaac tagttaatgt aaaaaatgca agagaaaatg
     1141 aagaattgat aattttatta atgtgtttat ttagcataac aaagaatgtc tatttccatt
     1201 aaaacctaac tatttttgat tactataata acccgtatta cacaaaaaac aaacatttaa
     1261 ttcacaccaa attagaaaat ttgctttagt aaggttgaaa atataactct aaaacacgat
     1321 aataaaaagc ttctctgcta acaatggcta gactgcttca tcgtcagttt cgccggtacg
     1381 ggtgcggata acttgttcaa tattcgctac ataaattttg ccatcaccca cttgcccaga
     1441 ataggatgca ttgagaatgg catcgatcac ttcatcggtt tggtaattag aaaccaatat
     1501 ctccacctta attttaggac gaaagtcaat gcggctcatt tggctaagct cttgttcggt
     1561 atgcccatat tggcgtccaa agccttttac ttcggtaata gtcattcctt gaataccgat
     1621 ttctgaaagt gcatcttgca cttggtctaa ttttgaaggt tggataatgg tagtaatcag
     1681 tttcatatta tatccttctc tgtttgagtt gccgtaagta taaaaagcta ttgaggggta
     1741 tgcaaggtag gaggtatttt ctccgtgaaa tattctgcca aatctccccc acatacaagc
     1801 ggttactttt tccaaaaatt ttgcaatttt tttatttttg cccttgaaca gtgcattttc
     1861 gcccttattt atggtttcgt ctgaacggga aatgcaatcc cgtttcttat tcaaactgaa
     1921 tttattatta gaggaatcat aaaatgggta aaatcattgg tattgactta ggtacaacaa
     1981 actcttgtgt tgcggtaatg gacggcgaca aacctcgtgt aattgaaaat gcggaagggg
     2041 caagaacaac gccatctatc atcgcttaca ccgataaaga aaccttagtt ggtcagccgg
     2101 caaaacgtca ggcaattact aacccgaaaa atactttatt tgcaatcaag cgtttaatcg
     2161 gtcgccgctt tactgatgca gaagtacaac gtgatattga aattatgccg tttgaaatca
     2221 gcaaagcaga caacggcgat gcgtgggtaa cggtaaaagg cgaaaaattg gcaccaccac
     2281 aaatttctgc tgaaatcttg aaaaaaatga agaaaaccgc agaagatttc ttaggtgagc
     2341 cggtaaccga agcagttatt actgtgcctg catactttaa cgatgcacaa cgccaagcca
     2401 ctaaagatgc gggtcgtatt gcaggtttag atgttaaacg tatcattaac gaaccaacag
     2461 cggcggcact tgcctacggt ttagattcta agaaagagaa ccaaactatc gccgtttacg
     2521 acttaggtgg tggtacattc gatatttcaa ttatcgaaat cgacaacttt gacggcgagc
     2581 aaacctttga agtgcgtgca accaatggtg acacccactt aggtggtgaa gatttcgaca
     2641 accgagtcat taactaccta gtagaagaat tcaaaaaaga acaagcggtt gatttacgca
     2701 acgacccaat ggcaatgcaa cgtgtgaaag aagcggcgga aaaagcgaaa attgagcttt
     2761 cttcggcaca agaaactgaa gtaaatcttc cgtacatcac agcagatgca agtggtccga
     2821 aacacttaaa catcaaagtg actcgtgcga aattagaatc tttagttgaa gatttagtaa
     2881 accgttcatt agagccatta aaaacggctt tatctgacgc tggtttatca gttagcgaca
     2941 tcaacgatgt aatcttagtg ggcggtcaaa cccgtatgcc attagtacag aaaaaagtgg
     3001 cagacttctt cggcaaagaa ccacgcaaag acgtgaaccc agacgaagcg gtagcaatcg
     3061 gtgcagcagt gcaaggtggc gtattaagcg gctcggtaac agacgtattg ttattagacg
     3121 taaccccact ttcattgggt atcgaaacaa tgggcggcgt gatgacaacc ttaattgaga
     3181 aaaacactac gatcccaacc aaaaaatcgc aagtgttctc aacagcggaa gacaaccaat
     3241 ctgcggtaac catccacgtg ttacaaggtg agcgtaaacg tgcagcggat aacaaatcgt
     3301 taggtcaatt caatcttgaa ggcatcaacc cagctccacg cggtatgcca caaattgaag
     3361 taactttcga tattgacgcg aacggtatca tcaacgtgtc tgcgaaagac aaaaacactg
     3421 gcaaagaaca acaaatcaaa atccaagcat cttcaggctt aagcgatgaa gaagtagaac
     3481 aaatggtgcg tgatgcggaa gcgaacgctg aagcagacaa aaaatttgaa gagttagttc
     3541 aaacccgcaa ccaagcagat ggtattgctc acgcaacccg taagcaaatt actgaagcag
     3601 gcgatgcgtt aaatgcagac gacaaagcga aaatcgacgc agccattgca gaattagaaa
     3661 ccgctgcaaa aggcgaagac aaagcggaga tcgaagcaaa aatcgaagcg ttaatcaaag
     3721 cttcagaacc gttaatgcaa gcagcccaag caaaaaccca aggcggcgag caaccacaac
     3781 aatcccaaaa agatgacggc gtggtcgatg cggaatttga agaagtgaaa gataataaat
     3841 aatcttgcgt aacttgtagg ggcgaaatat ctttcgcccc ttttgtgtag atatatacca
     3901 cctagcacga gccgtgctag gttacacata attcagcccc tttggggctg tgcaagcggt
     3961 gcttttcaaa ggaaattttg caaatgctaa aactctaccc tacaacggaa aatgtacagc
     4021 caccgtttta taaaatttac gatcataaaa gtggtgaaac ggtcattgct acagaaatgg
     4081 aaaacagcaa taccccgatt ttaatggacg attttgtttg gtttgagcaa attccaacca
     4141 ccgaggctct tttggtagat acttggtctg tgattgaaaa ccgtgctaaa caaggcttgc
     4201 tcaaacgagc ttacaccaaa gattttgaaa aacatttagc agacggcaaa gtagaaattt
     4261 atattgcggt gaatccgcat tgttaggctt tgatagcgca agcgtccacg cttgtgctta
     4321 accatcaaat ttggtacaag cgtggacgct tgcaccatct tgtctagaac ctattttact
     4381 ataaaacgct ctcccacaag gggcgagggc aagcggtcaa aaacagagaa aaatttacaa
     4441 acggaacaac aatggcaaag aaagattatt acgaagtcct tggactgtct aaaggggcaa
     4501 gcgagaaaga catcaaacga gcatataaac gtttggcggc aaagcatcac ccggataaaa
     4561 accaaggcag taaagagtcg gaagagaaat ttaaagagat taccgaagcc tatgacgtgc
     4621 tgaccgacag cgagaaaaaa gcgatgtacg accaatacgg tcacgccgcc tttgaacaag
     4681 gcggtgccgg tgcaggcgga ttcggtggct ttggcggcgg tggtttcggt ggttttgaag
     4741 acatcttcag cgaaatgttt ggcggtggct ttggtggcgg tggacgtcgc caacgtgtgg
     4801 tacgtggcga tgatttacgt tacgacatcg agatcaccct tgaagaagct gtgaaaggct
     4861 gtaaaaaaga tattcgcatt caaaccttag ctgaatgtga tacttgtcat ggtacagggg
     4921 cggaagcagg gagcaaagtg gaaacctgtt cgcattgtca cggctccggc agagttcgcc
     4981 gtcagcaagg tttctttgtg actgaggcag tttgtccaag ttgtcacggt acaggtaaac
     5041 gcattgaaaa accgtgccgt tcttgccacg gcgatggacg tgtacagaaa gcgaaaaacc
     5101 tctctgttac cattccggca ggtgtggata ccggtaatca acttcgctta tcgagagagg
     5161 gagctgcagg cgaaaatggc gcaccggcag gcgatttata tgtggtgatt cacgttaaag
     5221 aacacgatat ttttgagcgt gacggttcga atttatactg tgaagtgccg attagcttta
     5281 ctcttgcagc tttaggtggt gaaattgaag tgccaacttt agacggcaaa ttaaaactca
     5341 aaattccggc agaaactcaa accggtaaac tattccgtgt acgaggcaaa ggggtaacaa
     5401 gccctcgtgg tggctacgca ggggatttaa tctgcaaagt ggtggtcgaa acgcctgtga
     5461 atttaaccga agaacagaaa gcactattac gacaatttga agagagttta gaaggacaag
     5521 gtaaacatcg tccgaaacac gaaggtttct ttgatggtgt gaagaaattc tttgataact
     5581 taggcaaata aaattataaa aagtacatat ttcttaaata tgtacttttt tattatctat
     5641 tctttgttga aatctacttc tcggccgtta gtttttacac ctacatactg caaaaaatag
     5701 tggaaaaatg accgcttatc atctttctca gaatacttga caattaaagt caactattta
     5761 aaattaatat ttaatagtca ttaattatca aattgataga ttgagaaaaa atctacaaca
     5821 cctataattc tcttcggatt aattacctat cttatttcta atttatgagg attctattat
     5881 gtctaaatgt cctgttacac atttgacaat gaacaacggt gcacctgttg ttgataacca
     5941 aaacagctta acagcgggtc cacgtggtcc attattagcg caagatttat ggttaaatga
     6001 aaaattagcg aacttcgtgc gtgaagtcat tcctgagcgt cgtatgcacg ctaaaggttc
     6061 cggtgcgttc ggtactttca ctgtaactaa cgacatcact aaatacactc gtgcggcaat
     6121 ttttagccaa gtcggtaaaa aaactgaaat gtttgctcgt ttcaccaccg ttgcaggtga
     6181 acgtggtgcg gcagatgctg agcgtgatat tcgtggtttc gcattaaaat tctataccga
     6241 agaaggtaac tgggatatgg tgggtaacaa cacccctgtg ttcttcttac gtgacccacg
     6301 taaattcccg gatttaaaca aagcggttaa acgtgaccca cgcacgaata tgcgttctgc
     6361 aactaacaac tgggatttct ggacattatt accagaagca tttcaccaag tgactattgt
     6421 aatgagtgaa cgtggtattc cggcttcata tcgtcatatg cacggttatg gctcacatac
     6481 ttatagcttc atcaatgcag ctaatgagcg tttctgggtg aaattccatt tccatacaca
     6541 acaaggcatt aaaaacttaa ctaacgaaga agcagccgct attatcgcta acgatcgtga
     6601 aagtcaccaa cgtgatttat acgaagcgat tgagcgtggc gatttcccga aatggaaaat
     6661 gtttgttcaa attatgcctg aaactgatgc agataaagta ccataccacc cgtttgactt
     6721 aaccaaagta tggccaaaat cagactaccc attaattgaa gtgggtgagt ttgaattaaa
     6781 tcgtaaccct gaaaacttct ttgctgatgt tgaacaatct gcgttcgcac caagcaactt
     6841 agtgccgggt atcagcgtgt ctccggaccg tatgttacaa gctcgtttat tcaactatgc
     6901 ggatgctcaa cgctaccgtt taggtgtgaa ccaccaccaa atccctgtaa acgctccacg
     6961 ttgcccggtt catagcaacg ctcgtgacgg tcaaggtcgt gtggacggta actacggtag
     7021 taccattcac tacgaaccaa acagcttcgg caaatggcaa gaacaagctc aatatgcaga
     7081 gccaccactg aaaattaatg gtgatgcagc ccactggaac tatcgtgaag atgatgctga
     7141 ctacttcagc caaccacgtg tattgtttaa tatgatgaat gatgaacaaa aacaagcatt
     7201 attcaataat actgctgcag gtatgggcga tgcattagac ttcattaaat accgtcatat
     7261 tcgtaactgc tatgcctgtg atccggcata cggtgaaggt gtggcaaaag cattaggtat
     7321 gacggttgaa gatgctattg ctgcaagagc aacagaccca gcattaggtc aaccgggttt
     7381 actctaatcc ttagtgaata caaaactcaa agcaaaagct ctacgaaagt agagcttttt
     7441 tactgctaat tatttaacca ccgtaatttc ccacaaggca tcgccaagtt gttggtaatc
     7501 ggtaacttca tagccttcct cttccgccca atttggaatg gcttcggttg cttgagtgca
     7561 gtcaaattca atcgttaagc tatcgccttt attcaatttt gccatcgccg ctttggcttc
     7621 gactaatgga aacgggcaga caagccctga ggcaggtaaa cgtacgttca tctttattac
     7681 tccttaaaaa aattaacctt ttaaacgctg tgggcggata atcgtaaaat acgctccaac
     7741 aaatgtaccg agaatcatta ttggtaatga aagccagcct tgccaagaga agaaagctgt
     7801 ttcaactaat ccgttcccga ttgaacaacc accggcaagg cttgcaccga ttcccattaa
     7861 tgcaccgcct cctgcactgc gtaacattgt ggtcgcatct ggcactctga aacggaattc
     7921 attcgagaac tttgcagcca caaacgagcc gatcaaaatc cctaacacca aaaataccgc
     7981 ccagttaata aatttaactt caccggttac aaaatattga accaaatttg cacttggcgt
     8041 agtaatgcct aagccgaagt ttctaccggc agcactgcta agaggccaag caagcagtgc
     8101 aattaagcct accaacactg ccgtcaaaac gggtgccaac gtttttcaaa cagaatatga
     8161 gctaaaccgg ttttgcgtgg ttttaacgtg atggttttac ctctcggttt tcttaaatga
     8221 tagaaggtta atgcgaatgt aattacactc agaataaaca ctaacaccca aggggaaata
     8281 ccaaatgttt cataaatcgt gcggtgttca attacattgc tttttagctc ttgattaagt
     8341 ggagctaaca caccggttcg catagaagca gaaaacagcc cataaacaat cagagcaacc
     8401 caactgccaa ttaaaccttc accggctcga taccaagtac cggtagcaca accgcctgcg
     8461 taaacaatgc caataccaaa taaaaatgcc ccgataatta ccgcaagcaa tggtaaatct
     8521 tctgccggtg agctaaaaga gccggcttca tataacagcc aaatgccgat agattgcact
     8581 gtaatcgcaa ttaaaaatgc gacaaacata cggctgtttt tggtgacata gagatcacga
     8641 aatgcaccgg tgatgcaaaa tcgccctctt tgcatcacaa atccaagcag aaaaccgcat
     8701 aacaatcctg ttattaacat aaaaaatacc tctcaacaaa taaatgtgag aggtatttaa
     8761 atataaagcc gtctagatgg ctaataatct ttagcgaaat gatatgttat tcagttatat
     8821 caatgccatt aaatatgcgg aataagatca tcaggcccgc ctaatttatt caacgccact
     8881 cttcttgggt caactaactt agatttcttt atcatcggtt caaccgttga accttgtact
     8941 aaaattgaga aagtgaccac cgcataagtc attaatagaa tcaaatctcg cacatcaagc
     9001 tcgccttccg aacttgacac catcgcagcc cctgccggta cggaaagagc catcgcaagg
     9061 gctaatccac cgcgtaatcc gccccaagtc ataatgcgta aggtgtaagg attataagtg
     9121 cggaagcgtt ttaacacttg gtatggaatc cacaggctga tgtagcgtgc aagcaaacaa
     9181 attggaattg ctgccaacat taacacccca ccaataaagg tgaaatcaac taacagtgtg
     9241 cttaaaccga taagcaggaa taacagatag tttaatgaat ggtcgatcat ctcccagaag
     9301 tgatcaagat atttttggct ctgttcggag aaaccggaac ggcgagtcca gttgccaatc
     9361 ataataccgg caacgaccat tgttaatgca ccagaaacgt gtaagtagtt cgcaatcaca
     9421 aagccaacgg ttggaatggt taaggtgatt aagatctcaa ggcttccgtc atcggttgca
     9481 gaaataaatt tatgtgccac aaaaccggtt aataagccaa aaacaatacc gcctaccgct
     9541 tcttgcaaga acagtccggt aatgccacta agtgttggag cttgtccgcc aaaagccaca
     9601 gcaaagatag tggtaaaaat cactaagccg ataccatcat taaacaacga ttccccttca
     9661 acatgcattg aaaggcgttt tggagctttc aagcttttga taattgccaa tacggcaatc
     9721 gggtcggttg gcgaaatcag tgagccgaac aacaagcaat aaacaaaacc gatctccaaa
     9781 ccacataatt ttgccacgcc ataaatcaaa gcaccgatta agaaagtgga aattaaggta
     9841 gaaagtaaag cataaaccgc aatttctcgg cgttgttctt tcattaacgg tagtttaatt
     9901 ccaagtgaac cagcaaacag taagaaacca agcattccgt ttagtaggaa gcttttaaaa
     9961 tcgagctgtt caaaaatgga aatggcaagt ttatcaacat gaaaccagcc aagagaaccg
    10021 aaggtaagaa taagaaggga gccgacaagg gcggaggctg taatagcaat ggtggtttga
    10081 atattttcgt ttaattttcg ggtaaaaaac gaaataaaca tcgcaagccc aaacagcagg
    10141 cagatatacg agtagagttg cataacattt tcctataaat ataaaaactg ctgctagtgt
    10201 aaactctgtt taacagaata gctacctaaa aaataggcag aatacaattt gataacattc
    10261 tgcctatttt tggttatgcg tttgccaata atttttctaa aatttgataa taaatttcac
    10321 cgcaattcgc taaatcatcc acactgacac attcgtttac tttatgaata gtggtattaa
    10381 gtggtccaaa ttccaccact tctgccccca ttaacgcaat aaaacgcccg tctgaagtac
    10441 cgccgccggt gtctaaccga ggcgtaattt ttgccacatt ttcgaccgct tgtaatgcag
    10501 cttctaccaa cttcccattg ccggcaagga acggtttgcc cgacaaattc caacttattc
    10561 ggtattttag cccctgtttt tcgagcattt ccgtcacttt tgctttaatg atttcatcgg
    10621 ttacttcagt gcaataacgc aagttaaact ggatataaag ctcgcccgga atcacattat
    10681 tgctacctgt gccggctttg atattggcaa tttgcaaact ggtaggcggg aaaaattcgt
    10741 taccgttatc ccactggtaa gtggtcagat cggttaaaaa acccagtgac gtatgcaccg
    10801 gattttccgc caaatgtgga taggcaacgt gcccttgaat gccctcgata tagagatctg
    10861 ccgtaattga accacgtctg ccgtttttaa tcacatcgcc aaacaccttg ctactagacg
    10921 gttcgcccac taccgcataa tgtaccgctt cattgcgagc cattaaggtt tcaaccactt
    10981 ttaccgtacc gtctttggct gctgcctctt catcggaggt aatcaataat gccactttgc
    11041 ccttatggtt tgggtgatgt tgcacgaaag tttctgccgc cacaactaac gccgagagcg
    11101 agcctttcat atctgccgcc cctcgcccat aaagcattcc atctacaatc tcagccgaaa
    11161 acggcggata agcccattgg ctttcatcgc ctaccggcac cacatctgta tgcccggcaa
    11221 acacaatgca aggctctgtt ttaccatttt ccgagccgtg cgttgcccat aaattcaagg
    11281 tgtcgccaaa cggcaaccat tcaatattaa agccccgttt ttcaagacgt tgtgcaattt
    11341 cttgctgaca gcctttgtct tgtgggctga tggaatcacg gcggattagg ttctgtgcaa
    11401 gggagataat ttcatttttc ataggttaca agcggttgtt ttgggtgaat tttttgctat
    11461 atatcctgta ggggcgggtc ctgtgcccgc ccgaacaatc ctaaagacta cggacaggca
    11521 caggacctgt ccctacaagc aggggaaaaa attaattaaa cgcagccaca tactctttct
    11581 cattaaaacc aatcaaagca atatcctctt taatcacaat cgggcgttta attagggtcg
    11641 gctgttcggt tagcactttt aatgccgact cacggttaag gttgttttta atttcctcat
    11701 caaggtttcg ccaagtggtg ctacgtttat taactaagtt ttcccaaccg aattttgctt
    11761 ccatttccac taaccaatcg gcatttaaac cgtccacacg gtaatcgtgc agttgtgggt
    11821 tttgattatg atcttcgagc catttcaagg ctttttttac tgtgtcgcaa tttttaatgc
    11881 cataaactaa ggttgtcatc atttttcctc attatctgat tccagcattg cctgccagat
    11941 ttttaacgca tcggcggttt ctgccacatc gtgcacccgt aaaattttcg ccccattttg
    12001 agcggctaat aacgccccta ctacagagcc aagtagccgt tgttctaccg gtttatctaa
    12061 caccgcacca atcattgatt tacgggaaag tccggcaagc acagggaagc ctgaatccac
    12121 aaaggcttta aggtgtttca ataattggta attgtgtttc acgcttttac caaagccaaa
    12181 accggggtct aaaataatgt gttctttggg aatgcctgcg gtgatgcact cgaaaatccg
    12241 ttgatttaag aaatctgcca catcttccaa cacatcatca tagtgcggat tttgttgcat
    12301 tgtttgcggg ttgccttgca tatgcattaa acaaaccggc aagcccaatt ctactgccgc
    12361 tgccaacgca ttcggctcgg ttaaggctcg aatatcgtta atgatatcca taccgacttt
    12421 tgccgactct ttgaatactt ctgctttgga gctatcaacc gaaatcaagc aatcaaaacg
    12481 gctgcggatc gcctccacca ccggcacaac acgctctaat tcttgttcta agctcacaat
    12541 cgccgcatta gggcgggtgg attcaccgcc aatatcaata atgcttgccc catcagctaa
    12601 cattttttcc gcttggaaca aggctttatc gagggaaaaa aacttgcccg aatcggaaaa
    12661 tgaatccggc gtgaagttta agatccccat aatttgcggt gtggttaaat ctaacaagcg
    12721 gtcatttttt tgaaaatttt tgaaatggat ttgcatattt ttgcttatat tttgttggtg
    12781 caagcgtcca cgcttgtacc tctctatgtt gatttacagc acaagcgtgg acgcttgcgc
    12841 tatcttactc tctcattacg ttatttagta attagccctt aattttgcca acaccccttt
    12901 taaggtttta tccaaaggtg cggtaatgac catttcttcg ccggttttgg ggtgttcaaa
    12961 acgaatagca tgggcgtgca gaaataaacg ctgtaagcca acttctttca ttttgctgtc
    13021 aaattccaca tcaccatatt tgtcatctaa cgcaatcggg tgtccggcat attgggtatg
    13081 cacacggatt tggtgggttc tgccggtaat aggcgaggct ttcaccaaag tggcattggc
    13141 ataacgctct tcaatgctga aacgggtttc ggacggtttg ccctcctcac tcactcgtac
    13201 aattcgttca ccgctggcaa gttcgttttt cagcaacggg gctttaatca ctttagtgtg
    13261 agcctgccat tgccctcgca caagggcaag atagtctttt tgcaccactt tctcacgtag
    13321 ttgttcgtgc aaggaacgta acgccgaacg cttttttgcc accagcaaaa tgcctgatgt
    13381 atcacggtcg atacggtgta ccaattccaa gaaacgagcc tgtggacgta acgcacgcaa
    13441 agcctcaatc acaccaaaac ttaaaccact gccaccgtgt acagcaatac ctgatggttt
    13501 gttaattact agcattgagt catcttcata cagaatttgg ctttccagct ccgccacttt
    13561 gtttaattta gtcgaaattg gggcttcatt tttttccgct acacgcaccg gtggcacacg
    13621 cactaaatcg tcgggctgta atttatattc gggctttatg cgccctttat tcacccgcac
    13681 ctcgcctttt cgcacaatgc ggtaaatcaa acttttcggc acgcctttga gctttgccaa
    13741 taaaaaatta tctaaacgct gtccggcttc atcttcactg atcgtaaaaa attgaacacc
    13801 ggcactaatt actttttctg ttgtcattat cttgtctttt agagaaaatt ttgctctaaa
    13861 ttctagcaca aaaccgctta gcgagtgagg caaattcgct aggcttattt ttcattttaa
    13921 ttttaaggaa acaatatgac tgaaaaagtc accgaaaccg ccccaaatat tgacgtggtt
    13981 gccgaaaccg aaaaagtaat taacaaagtc agcacaatgg attttaattc cgtattaaac
    14041 gagtggatta tcccttatgg caccaaaatt ttactagcga ttgcgatttt tgtagtcggg
    14101 aaatttcttg cccgcctgat tagccgttta ctcggcaaag cggcattaaa atcaaccaaa
    14161 gatgaaatgc tgcaaagttt tattgtctca atcagctatt ttttactgct gttaattgtg
    14221 gtgattgcct cgctttcaca aatcggcatt aacaccagct cgctggttgc attaatcggt
    14281 gctgccggtt tagccatcgg tttatcatta caaaactcat tacaaaactt tgctgccggc
    14341 gtgatgttgt taatcttcaa gccatttcgc aaaggggatt taatcgaaac cggcggaatg
    14401 accggcgtgg ctgaacaaat ggggctttta gtgttggaac ttcgcaccgg cgataacaaa
    14461 accgtgttat taccaaacgg caaagtgttt tctgacagca tcatcaacta ctctaacaac
    14521 ccgacacgcc gtattgattt cacctttgat atttcttatg aatctaactt taaaagaggc
    14581 gaaagagatt atccaacgca ttttagacgc caatacttat gtattaaaag atccggcacc
    14641 tgtagtagca gtcggcgctt tagcaccgca tagtgttcaa cttgttgttc gcccttgggt
    14701 tcagacgcca aattactggg cagcatattg ggaaattatc gaacaagtaa aattagcctt
    14761 tggtgaagca ggtatatcta ttccttacaa ccaaatggaa ttgcatatta aaagcggttc
    14821 actagagaat aaagcataag aaaaaagcct gtaagcggtc ttttttcgat aaaattttgc
    14881 aaattttggg cgaaggttta tactttagct aaataatcta cgaacaggag catattatgc
    14941 gtattgatac ctctgcactt tgcgatcttt attctgacca agttgatgtg gttgaaccaa
    15001 ttttctcaag ctttggcggt gcttcttcct tctttgggaa gattaccacg gttaaatgct
    15061 ttgaaagtaa cggtttaatt gctgatgttt tggaagaaga gggtgaaggg cgagtgctgt
    15121 tagtcgatgg tggcggtgca gttcgtcgtg ccttaattga cgcagagctg gctcaacttg
    15181 ctgtagataa cggctgggaa ggtatcatcg tttatggtgc ggttcgccaa ttagatattt
    15241 tggaaagatt agatatcggt attcatgctt tagcgccaat tccagtggga gcagataaca
    15301 ccgatattgg agaagtggac acaccggtca atttcggtgg cgtaacgttc ttccctgaag
    15361 attacattta cgctgattta accggcatta ttctttcgca agagttgttg gatttaagcg
    15421 atattagcga ataaatattt gcaaaaaaat accaaaaacc gaccgcttgg tcggtttttt
    15481 actactgcaa tatttttaat gccgcattat attcttgaga ttgctgtaat aaaactcgag
    15541 cggcttcacg taatttatct acatcgtctt ccggcagata aaatgatgtt gcaatattta
    15601 gcacatctct ttttaaatca ccatcaggta attcttttaa acctaaacta acaaaatgca
    15661 tttcgatctt cgatggctgt ttatttgcat attcattcca accatccgta aactggcgat
    15721 aacgctgtaa attaatttgt gtcgttctat caattggcac gttaactatt gtattcacta
    15781 catcagccac acccggcaca tttgcggtct tatcgatctc gctagagact tcattttgcg
    15841 catttacatt aatcactaca atatttttca ccccaagctg atgaatatac aggtacatct
    15901 cttcaatacc cataacatca gatatttcca gcacactggc aagccctaaa ttatccgtta
    15961 atccaccatc aactaaatgg ataaaggggc gctctttact atcttgataa aggctgagta
    16021 tatgattcgt ttcttcaata ttttttgtaa ttagtccatc aattgctttt tccctagcac
    16081 taaattcttg tggcatacta aaatggcaat ttccaccatt gttattcagt gtaagcggcg
    16141 caaaaatcag aggaacagaa ctagaagcag caacagcacg ggaaatttct aaatcattca
    16201 aatttaaaca aagcccatca aaggtttctt gcgtaaaggt cagtttttga ccaatattca
    16261 tgtctgttgc gctaatcaca gcaaaaggtc ctttacgatt tttcactaaa tcaccaaact
    16321 ttttaccatg ataaagggca aaatttaagc gttcttgcaa taaatcacca cgcccatatt
    16381 gaggagaact taaacgagga agattggata acgaaaacac ctctgtaata atatcttttt
    16441 gaaagtttgc ttttaaaaaa cgatcagcaa atttaggaat gatatccttt ccttccaacg
    16501 caaaataagt ggctaatatc gatccacctg atacgccaaa ggcaatatct atattctcta
    16561 ataaagtatt acctttattt gtttctttca cccttacctt tttaaactct tccaacactc
    16621 cataaccaag cgccgctgct cttgttccac cacctgaaaa catgataaca acaatattac
    16681 cgtcttttgc actttcagcg atcacatttt tttgtcgata acctctttca acatttacct
    16741 gtttaattgt ttcaactggc ttataaacaa ccaaactgca agcagataac actagcgtaa
    16801 aggcgaagat gaaaagctgt tttattttgt ttagaaaaag cataaaaacc ctaaattaag
    16861 atctatttct acaaattata acctttttag ctaaagataa atgcggtcaa atttggccta
    16921 aaatttgcaa aattatttaa tataagtaaa tagtgctttt catcataagc gctttatgaa
    16981 acagattgaa agggataaaa tgcctatatt ctctatgtac taaggaatta taaaatggca
    17041 aaacaaattg cagtattaat tggtagcggc agcaaaactt catttagtca cttgactgtt
    17101 cgtcacttac aaaaaatggc acctgcaagc attcaattaa acattgtgga tattgcagac
    17161 ttaccacttt acgaccgtga tttagatgaa aacagcccgg cacaatacac ccgagtgcgt
    17221 gaagcggttg ctgtggcaga tggcgtgtta ttagtcacac ctgagcataa tgcaagcttt
    17281 tctgcaatgc ttaaaaatgc gattgacgtg gtttctcgcc caatggggca aagcaaatgg
    17341 attggcaaac ctgcaggtat cgtaaccgtt ggtgcaagcc cagcaggtgg tgtgcgtgcc
    17401 gctgatcaag tgcgtactat tacttcaggc tcatttatca atatgccaac tttacctttt
    17461 gcattaaacg taggtaactt atttgcaggc gtatttgatg agcagggtga aattgcctct
    17521 gaggcattaa aaagtgcaat gcaaaacttc attaatgcct atgctcagtt tgtagaaaaa
    17581 tactaatctt ataatgcaaa aaagccgttt atctaaaaat ataaacggct tttttcgtag
    17641 taaaagaaag gataattaaa gagcctgttt aggttttacc acaccctgca gtacctccct
    17701 taaccaccga tgtgccgtat cttgttcggt tctttcgtgc caagccatca tcatggtgaa
    17761 gccttctacc tctataggcg gaggttgtaa ataaacattg ggtaaggtct ttgctaagcg
    17821 ttcgggcaat actgcgacta agtcggaacc ttgtagcagc tggggcagca gggaaatatg
    17881 gtttaccgag accattactt tgcgctgtag tcctatcttt tgtaacgcta gatccaatgt
    17941 tggcggctga ccaaggcaag atcgattttg ttttgctcga acaaggtttt gatattttgc
    18001 ccctgcactg gcagcaaggc gacttttaca ttcggagcaa gccgtcttaa acgcagaatc
    18061 agcggcaagg caataatttg ttgtacataa tccattgcgg caatacgcaa ggtcatattc
    18121 agatgttccg gttccaatat gggtggttgc agcatggagc tgatatcttg cagaatgttg
    18181 cgtgcggttt gccccagttg caacgcgcgg tcggttggct gcataccgtg ttgcactcgc
    18241 acaaataacg gatcgttaaa actctcgcgc aagcggttta aaataccgct cattgccggt
    18301 tgggtgatgg ataatcgttg tgctgcccgg ctaacgttgc attcgtccag cagcaccaca
    18361 aaagctttga gcaaattgaa atccagtgtt cttatatcac tcatttttat acctaactat
    18421 cacaataatc aatttgaata gataatagca ctgaattatc attccaccta atctatttca
    18481 ttaaggaagg caacatggcg aagttcagaa aattattcac cccgtttaac attaaaaact
    18541 tagcattgaa aaaccgtgtg gttatgccac caatgtgtca atattcggca accaacggcg
    18601 taccgaacga ttggcatttc gtgcattaca ccagccgtgc agtaggtggt gtcggcttga
    18661 gtattgtgga gatgaccaat gtggcgccaa acggcagaat tacgccaaac tgcttaggct
    18721 tatggaacga tgaacaacaa caggcattca aaaaactggt agacagcgtt catgctcaag
    18781 gggcaaaaat cggtgtgcaa atcgcccatg ccggacgcaa aacccaagac gagccggatg
    18841 cagtggctcc aagtgcaatt cactacggtg agttggatta tgccggacaa aatctgatta
    18901 cacctcgcga gctgacgaca gacgaagtca aagcattggt tcaagccttt aaggattcgg
    18961 tcaaacgtgc cgttgaggca ggctttgata caattgaaat ccacggcgca cacggttatc
    19021 taattcacca attctattcg ccgaaatcca atcagcgcag tgatgagtac ggccaagaca
    19081 aaatgttgtt tggcgagcaa gtgattcagg cagcacgtga agtgatgccg tctgaaatgc
    19141 cgttaattgt ccgcatttct gcacaagaat acggagaaaa cggctttggc ttggattatg
    19201 gtttgcaggt agcgaaacgt tttgccgatg caggcgcaga tgtcttagac atcagcggtg
    19261 gtggtgatgg tgtattgcat gcaggtaatc accccgaatt ttacgcaggt taccaagtag
    19321 actttgcccg tcaagtgaag ctcgctacgg gcaaaccggt aattgcagta ggtatgttgg
    19381 ataatccggc ggtaggcgat catatcttag gtatgggcga tgcggattta gtggctgtgg
    19441 gaagagcctt attgcgcgat ccgtactggg tattgaatgc gcaatatgac caaaatgcag
    19501 cagacagcag tgaaatgcag tttgtaccga agcaatatga acgcggatta tttatgtaaa
    19561 caaccaacgt taaagaccgt ctgaacattc agagggtctg ttttttctca tcacgtcaac
    19621 ttatacctaa atatcaaaat tatcaattat taaaaataac ttaaatcttt tatacttatc
    19681 cgtctttcat ctagtctttt cagaccgtct gaaaagactg ttttgcattt ttaggagtgt
    19741 tccatgcaat ttttcaacaa agaagacgta atcaatgcct ttcattaccg tgcatctacc
    19801 cgttcttacg acggcagcag aaaaattccg gctgaagatt tcaactatat tctggaactc
    19861 ggtcgtttaa gtccaagttc ggtaggctca gagccttggc agtttttagt gttgcaaaac
    19921 cctgaattac ggcaaaaaat caaaccttac tgctggggca ttccaactat ggaaacctca
    19981 agccatatcg tggtgatttt agcgaagaaa aatgcccgtt acgacagcca atatttttct
    20041 gaaattatgg atagacgagg tttgcaaggc gaagcgcgcg aaaaagccat ggccgtgtat
    20101 aaaaaattcc aagagaaaga tatcggcatt ttagacagcg aacgcagcct gtttgactgg
    20161 gcaagcaaac aaacctacat cgcattgggc aatatgatga ccggtgcagc tttaattggc
    20221 gtagattctt gtccgataga aggtttcgct taccaagaag tcaacgatat tttggcagcg
    20281 gaaggattat ttgacccaag cgaatgggac gtatcggtga tgtgtacctt cggttaccgt
    20341 gataaagaaa tccgccaaaa agcacgtaaa gatttcgaaa ccgtggtgaa attcatcgaa
    20401 taaaccggct aggcaaaacg gctattcaac atcaggtaaa cgccgatacc aagccttaag
    20461 gccgtctgaa tccgcattca gacggcattt cttttcaacc accactcatt ttgaggcggc
    20521 aacacaaccc agttagtcat gacatttatt atcggtattt tagccggcgt gctgattgca
    20581 tttcaatcag ccatcaacgc ccgtttgcgt atgcacgtgg gtgcggcatt tttaacctcg
    20641 tttgtctctt tcagcgtagg cctcctgttt ctactcggac tctcgctcat catgcaacaa
    20701 acaccagcat ttagcctcga caatttaggc aacgtcccct tatgggcatg gacgggcgga
    20761 ttactcggca tggtggggct aacggcaaac gtactgatct tccccaagct cggcggcgta
    20821 caaactgcgg tcatgccgat tttaggccaa gtgctgatgg gcgtgctgat cgacactttc
    20881 gcttggctta actcgcctta catagccttc acggccagcc gcctgtttgg cattggttta
    20941 gtcttattcg gcgtatggat tgccgtggta ctgccccatc tgtccggtct aaaacgcaaa
    21001 gaaaacgcaa atttatggct gtggcgcgca gtcggcatat taggcggaac cacattagca
    21061 acccaagccg ccgttaatgg cgaattcggc agacagctcg gctcatcact cggcgcagca
    21121 acagtctcct tcggtgtcgg tgccatcggc ttattactgg tagtgctgtt ttatgaaaaa
    21181 agcctgccga aactctgcca gccaaccgaa ggcaaaccgt tttggatatg gagcggcagc
    21241 atactgggca gcctgtttat cctcgctggc gtttggctcg tcccccaaat cggcacaggc
    21301 tcggtggtga tgctcgtcct ctctggcctg atttgcggca gcctgctggt agataaattc
    21361 ggtctgttcg gcattacaca aaaacccatt ctaccgctgc agatcatcgg cgttacctta
    21421 ctcttagccg gcgtcgcatt tatccgctta ctgtagccaa gctgacacct cttgcaaaat
    21481 aacccaaaaa aaccaccgct tgtgatcggc aagcggtggt tttcttttta aaatttgcaa
    21541 aacaactttt cagacattgt gaaaatgtat ttatgtaaat ttagcaaaag tgttttgcga
    21601 ttgatgtgat ttttaaaata aattcattgc attaaaatac gcccattatt ttcacaaaag
    21661 gtcaaaacaa tgagcaaaca aatccaattt accaaaacag gttcgcctga tgtcttgcaa
    21721 atcgttgatg tacaaatccc cgcccccaaa gcgtttgagg tacaaatcca aatccaagcc
    21781 attggcttaa accgtgccga aatgatgtac cgtgaagggg cgtatgtgat tgaccctgta
    21841 ttccctgcga ctttgggtta tgaaggggca ggcgtggtcg tggcgattgg cgagggtgtg
    21901 aacgagcttg ccattggcga taaggtcagc attattccat cttttatgtt taccgaatac
    21961 ggcacttatg gcgaaatcgt caatatgccc aaacacgccg tcgttaagca ccctgataat
    22021 ttatcaatgg aacaagcgtc cgccagttgg atggcatttg ttaccgctta tggcggactg
    22081 attgagtttg acaaagtaca aaaaggcgat gtacttttgg aaaaaggagc ggattttgtg
    22141 attgccacaa gcgaagacga tgtaaccgcc aaactttttg aaatcaccaa tggcaaaggc
    22201 gtcaatgtgg tatttgaccc tgtgggcgga aaagaggcgg caaaaatcat caatgcaatg
    22261 gcacaagatg gtcgttacat catttatgga gcgttaagcc atgatgatat tgctgtgcca
    22321 gtgttcccga ttttgggtaa gcatttgacc gtgcgggggt atgaactctt tgaaattacc
    22381 accgtacctg aaaaattggc acaagccaaa acctttgtct atgacggttt ggcaagcggt
    22441 cagttacgcc ctgaaattga caaaatcttt gcctttgatg aaatggttaa agctcacgaa
    22501 tatatggcaa gcaatcagca gattggtaag attgtggtta aggtttgatg atttgaaagc
    22561 accgcttttg tgatgaaagc ggtgtttttt atggaaaatt ttaccagtta tttctaaaca
    22621 cttttcaaca cctgcaccat ttctatccac gcctcaaaca cctgtggaat gggcaaaagt
    22681 ttgccttgtt caaaataata tttatggctt tcaagggcga tggtggcaaa aatatgggct
    22741 tgataatccc aatttttctc agcccaattt ttctcagccc agtttttatc agcccaattt
    22801 ttatcatctg atgttatccg tttggcgtgg gcgataaagg cgttttttaa cagcgtgtgc
    22861 atatcgtcat acaaatgatg gcgtggggtt ttgatttgcc aaagtgccaa aatcagccgt
    22921 cttttttcca ataagcggtt gccaatggtt tgcataaatc gcagtaaatc atcgctttca
    22981 aaacgctttt tgagcaggtc ttgatattcc gccttcacct cttttatcaa tttgccgacc
    23041 aaatcgcttt tgcccgaata ataacggtaa aaagtggtgc ggttaatcaa ggctttttca
    23101 atgatttctt ggacttgcac tttttcaaac ggcttttcat ccaataactc aatcaaagcg
    23161 ttttggattg ccagatgggt tttgattact cttaaatcgt tcatcttttt tcaaaataag
    23221 catacatttt tataatttta tctgcatatc ctgctaaata cgagcttttt aatgtttgtg
    23281 aaaagtgacc gcactttcta caatatgccc acgatttttt aatgagaaca caaaatgatt
    23341 aaagtacatt gtctgacaac aggttgggta caaatcaaaa ttcatcatca agtggcacgc
    23401 ttttttgccc atcctgctcg tgtgcttgat gtgctgaccg ataaaaaatg gtcgcccaaa
    23461 ctgccgattg gctgttggct gattgaacac gatgacggtt tgattttggt ggatacaggc
    23521 gaaagttctt ccgccaacga caagggttat caaccgtggt ggcatccatt tatgcagttt
    23581 tgcgaacggc gtggggtcaa aaaacacgaa gaagtgggcg aaattttaaa ggcaaacggc
    23641 tttgaccctt tacgcattga cacggtggta atgacccata tgcacggcga ccacgcaggc
    23701 ggattgccca atttcccaaa cagccgattt gtgatgagtg agactgaaaa acaggcaatc
    23761 aacgccccaa atgcggtgat gaacggttat ttgacaatgc actatcctga ttggtttaat
    23821 ccttgcggta tcgcctttaa tgatggggcg tttgaaagtt ttgaacaaag tcataaactc
    23881 accaaagacg gcaaaatccg ccttgtgcca accgcaggac atacgctggg gcatttggcg
    23941 gtggtggtgg atatgggcga gtatgatatt ttgattggcg gagatgcctc ttactctgaa
    24001 caagatatgc ttgctggcaa cattgacggc gtgtgcgttg acggacatct gcaccaacaa
    24061 agcaccgcca aaatgcgtca gctttgccaa agaaaaccga ccattaccca atttgctcac
    24121 gatgaaaaga gtgaatatcg cctgaaaaat aaggtgttta cggtggtaaa aaattaaggg
    24181 ggaatgaccg cttgttgaaa ggcaagcggc catttttatt aaaaattttg caaattttca
    24241 gtcagcttga aagcgtctat ttatgccaat ttagcaaaac actttctcaa tttatcccat
    24301 tttaagcaaa ctcaaaagcc cttaaaatac cttatcttaa ataagacagg tacgccaaaa
    24361 tgaacacgct ttcccccatt ttctcccaag tttttgggca aaatcaatta acactgggct
    24421 taattgcccc ctttatgggc tatcaaaatc acattccaga caatttgacc accattgccg
    24481 acaatgcacg gctggctgaa aacttgggct tttcagcatt gtgggtacga gatgtgccgt
    24541 tttatgaccc tcattttggc gatgtggggc aagggcttga cccgatggtt acacttggtt
    24601 ttttgtccgc ccacacccaa aacatcgccc ttggtacggc agggctgatt gcccctttgc
    24661 gtagtcccat tcacattgcc aaaatggcga caagtgtgga tagtttgagt catggacggt
    24721 ttttattggg cttatcctct ggcgatagac ctgtggaata ccctgcattt ggggcggatt
    24781 ttgcctctcg tagcgagcgt tttcgtgaaa gcattgattt gattaacacc ttaaccgagc
    24841 aggattttcc ccatttcaaa ggcaaacatt atgccgattt aacagacaat ttggatttgc
    24901 tccccaaacc caaacaccgc ttgcccattg tggcgattgg acgagcaagg caagaaatgg
    24961 actggcttgc taatgtacct gatgcgtgga tttggcacgg tgtgaatcct gccgacaccg
    25021 caaacatcat caacaccctt gccgatttac gccaaaaaga cacccccaca gcatttggct
    25081 atgctcaatt tgtggaagtt ttggacgaca aaaacgccaa agcacagtta tttaacaaca
    25141 tttatctgcg tggtggtgca aaaggcttgg cagacttttg ggcaaaagaa caagaaaaag
    25201 gcgtgaaaca tttggcactc aatttaaaac ccactcagcg tcctgctgat gaagtgctta
    25261 cagaattggc agagtatgct ttgacacaat t
//
LOCUS       DANJWC010000016        25231 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.016, whole genome shotgun sequence.
ACCESSION   DANJWC010000016 DANJWC010000000
VERSION     DANJWC010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 25231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25231
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.016"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(70..342)
                     /locus_tag="PWX02_000472"
     CDS             complement(70..342)
                     /locus_tag="PWX02_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005819506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDL1728515.1"
                     /translation="MNKTELIDAIAAGANLTKKDAKAALEATLDAISASLKAGDAVQL
                     IGFGTFKVNERKARTGRNPKTGEEIKIAAAKVPAFVSGKALKDLVK"
     gene            complement(478..1068)
                     /locus_tag="PWX02_000473"
     CDS             complement(478..1068)
                     /locus_tag="PWX02_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF416 family protein"
                     /protein_id="HDL1728516.1"
                     /translation="MRNPIHKRMEHFEPWQNTTFMACLCERMYPNYQLFCEVTEQPEK
                     AKVFQNILNLVWEYLTIKGAKINFDNQLEKLEEIIPDVNEYAFFGVLPAQEACEALSE
                     LLHSIIAGSTLEQAIRISQISLGTVASYLEMQQDKELNEQELKNSEEIQEELDVQWQI
                     YRLLNECEKRDLDLIFELKDEIRSSGISNIGLNINQ"
     gene            complement(1215..2279)
                     /gene="hemE"
                     /locus_tag="PWX02_000474"
     CDS             complement(1215..2279)
                     /gene="hemE"
                     /locus_tag="PWX02_000474"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDL1728517.1"
                     /translation="MSELKNDRYLKALLREPVDMTPVWMMRQAGRYLPEYKATRAEAG
                     DFMSLCRNADLACEVTLQPLRRYELDAAILFSDILTVPDAMGLGLSFGAGEGPKFARP
                     VASLNDVNQLPIPDPEDELQYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTKIKKMLYAEPHILHKLLDKLADSVILYLNAQIKAGAQAVMVFDTWGGVLAHED
                     YKAFSLHYMHKIVDGLIRENEGRKVPVTLFTKGGGLWLEAIAETGCDAVGLDWTVDIA
                     DARRRIGDKVALQGNMDPSVLYASPERIEQEVKNILAGFGNGSGHVFNLGHGIHLDVP
                     VESPKVFVDAIHNYSKQYHK"
     gene            complement(2377..2934)
                     /locus_tag="PWX02_000475"
     CDS             complement(2377..2934)
                     /locus_tag="PWX02_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HDL1728518.1"
                     /translation="MAITDSQQFKQLVSQLQIDDTPAEFHGFLCGLIAGGIQDESWKT
                     LTYQFTNDGHAFSIEPLNTLTDFYQQLSESFSEANTLFSLWLPQNEEDGFALADGISE
                     WTNSFLLGLGVAQPKLQQETDEVGEAIDDLDEIAKLGYSDDDNNEELLEAGEEVLEYL
                     RVLALFLHSHFALGVPKTEEKPPLH"
     gene            3013..3303
                     /locus_tag="PWX02_000476"
     CDS             3013..3303
                     /locus_tag="PWX02_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDL1728519.1"
                     /translation="MSANNIELQIFGQVLRLQCPVEQQEHLLASAQRLEERVAVLKEQ
                     SGIIQLEKVLAIVALNLNYELEQAQRQNAENKNVLVACIEQLDNSLGKFQSN"
     gene            3408..3599
                     /gene="ssrS"
                     /locus_tag="PWX02_000477"
     ncRNA           3408..3599
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PWX02_000477"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            3601..4179
                     /locus_tag="PWX02_000478"
     CDS             3601..4179
                     /locus_tag="PWX02_000478"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDL1728520.1"
                     /translation="MNQSSLTDLRSQLRQSMRLKRQSLTAGQQTQAAESIIPQALSLI
                     ESYQASHIAFYLPFNGEISPIPLMEQLLQQGKKLYLPLLHPFTSGQLLFVNYNCKTIL
                     KFNRLGIQEPILDVRNILPLQELEMIFTPLVACDKAGNRLGMGGGFYDRTLAQAPHLI
                     SVGLAHECQQVEQLPIESWDMPLDHIILGAAK"
     gene            4176..4964
                     /locus_tag="PWX02_000479"
     CDS             4176..4964
                     /locus_tag="PWX02_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDL1728521.1"
                     /translation="MNAKKEKESSGNQSLIRGLTLLELLAKFPNGCPLAHLAELSGLN
                     KSTVHRLLQGLQQEGYVRPAPTAGSYRLTTKCLAIGQRALSSINILHIAAPHLEALNL
                     TLGETVNFAMRENDHAILINKLEATTGMMRTRAYIGQQMQLYCSGMGKLFLAYGSADY
                     IPQYWQTKSDIIQKLTVNTITTIDGMQTELETIRRQGFAMDNEENELGVSCIACPIFD
                     HQQKVHYAISVSLSTARLNQLGRENLLPAIQETAEKISAELGGR"
     gene            5058..6572
                     /gene="putP"
                     /locus_tag="PWX02_000480"
     CDS             5058..6572
                     /gene="putP"
                     /locus_tag="PWX02_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HDL1728522.1"
                     /translation="MFGLDPTTIMFVVYILGMIAIGFIAYRVTNNLSDYILGGRRLGS
                     FVTALSAGASDMSGWLLMGLPGAVYAGGLIEGWIAIGLTIGAYLNWKLVAGRLRSHTE
                     HSGDALTLPEYFHNRFGDKTRLIKILSAVIFLLFFAIYCASGVVAGARVFENLFGLPY
                     HQAIWYGAVATILYTFIGGFLAVSWTDTIQASLMVFALILTPFFVMYNLGGFDEMQAL
                     IDRAGEMAQKDYNDIFTGTSVLGIISLMAWGLGYFGQPHIVVRFMAAESVKSLENARR
                     ISMTWMVICLLGAIGIGYFGMAYFFGHPEQVGNISKNPEQIFIELAKLLFNPWVAGIL
                     LSAILAAVMSTLSAQLLICSSAITEDLYKGILRPQASDKELVWLSRLMVLAVAALAIY
                     IAQDPDSKVFGLVRNAWAGFGSAFGPVVLLSLFWKRMNGFGAIAGMLTGALIVHFWGD
                     ITLKLALPEIYSMIPGFIAATLMIIVVSLITPMPSKAVLDTFDRANKAYLAEVK"
     gene            6724..7890
                     /locus_tag="PWX02_000481"
     CDS             6724..7890
                     /locus_tag="PWX02_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1728523.1"
                     /translation="MTNLLLAIIYLSFISLGLPDAILGSAWPSMHQQLAVPLSYAGVV
                     SMIMSIGTITSSLMSDKLTYHLGTGKVMVISVALTALALWGFSISDAFWILCLWAIPY
                     GLGAGAVDAALNNYVAVHYASRHMSWLHCMWGVGASIGPHILGYALLQGRGWEAGYFT
                     ISMLQIGLTLMLFCSLPFWKLRKQITAEINHSAKPMSLKETLKIPGVKYGILAFFGYC
                     GVEQTAGLWASSYFVMVKDVGLEQAAAFGSIFFIGVTVGRGVSGFLTLKFSNRQMIKL
                     GSALILVGIAILCLPLGKYVTLASLIIIGLGCAPIFPCLIHSTPAFFGEDKSQSVIGV
                     QMASAYIGVLLLPPIFGFIANAISIQLLPLYLLLMFAIMAYAYRNLVRETSPSP"
     gene            complement(8135..9007)
                     /gene="rsmI"
                     /locus_tag="PWX02_000482"
     CDS             complement(8135..9007)
                     /gene="rsmI"
                     /locus_tag="PWX02_000482"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDL1728524.1"
                     /translation="MKNRQNSSEINPNYGILYIVATPIGNLGDITQRALDTLSSVDLV
                     AAEDTRHSGLLLSHYGIKKPFFALHDHNEQQKAVVLVEKLAKGENIALISDAGTPLIS
                     DPGFHLVRHCRQAGVKVVPIPGACAAVTALCASGIASDRFCFEGFLPAKTKSRCDKLT
                     ELENEPRTLIFYESTHRILDTLADMQTVFGEDRYVVMAREITKTWETIYGDQLGNLIE
                     WLNEDSNRIKGEIVLVVEGKPQSSEEAFSSQAIKLLTLLCQELPLKKAAAIVAETFGY
                     KKNALYQYGLEHFE"
     gene            9081..10802
                     /locus_tag="PWX02_000483"
     CDS             9081..10802
                     /locus_tag="PWX02_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HDL1728525.1"
                     /translation="MATILNHTMKKALVPTAIALFISACTSVNPVTESIKNEAYSSSE
                     FYINKADQTKEAEDKISYQLLAVRKLIDENKEYEAQNTFSEILTAEMNEVQKLEYALV
                     SAQLAALQGKNEQATSQLKAIQDPLLSSAQRLRYIQTQARIAANQKDVIGIVRARSQL
                     NNFYKMNRERQENNDIIWQTLRDANRGMLEKTVPEAGEMELAGWLALINIYNQNVTTP
                     AQMPQAINNWKAQYPSHSAVAVMPTELQGVSNFQQTQLNSVALLLPLSGDAKILGDII
                     KRGFNDAKADDSTAVQTFDTDSSDVNSLISQAKQQGANVIVGPLLKSRVDEMLLSPEI
                     QNVNILALNATENVRNIAQVCYYGLSPESEAQSGAEKIYQDGNSVAIVAAPQDDYGNR
                     SAEAFAKRWRQLTNSDADVRYYNQPLDAVAAIQNAGVSKAALYILGNAEQVLEIKQGI
                     DSSTLKDRLAIYTSSRSNSPNNGIDFYTSMEGVKFSEVPLLADQSSSEYKKAENLANS
                     DFSMMRLYAMGADAWSLATKFNEFRHIPGYKISGLTGELKAGQHCNIERSLSWMQYRN
                     GAIQQAY"
     gene            10777..11148
                     /locus_tag="PWX02_000484"
     CDS             10777..11148
                     /locus_tag="PWX02_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDL1728526.1"
                     /translation="MELFNKLINKREQGSFYEQKARVFLEQQGLRFVAANQAFKGGEL
                     DLIMQDGQTVVFVEVRQRKSNRFGSAVESIDYRKQQKWQNAANMWLLKQNQSLETANC
                     RFDVVAFEGNQTPVWIPNFLG"
     gene            11154..11738
                     /locus_tag="PWX02_000485"
     CDS             11154..11738
                     /locus_tag="PWX02_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL1728527.1"
                     /translation="MLNKIQDRFTESIQTQISAAELLPAKLSEAANRIVACLLRGDKV
                     IVCGHGRSYGNAELLVSHLLHRYELARPSFAAVQLQLSGVLAGVVAQDNELEAVYKKQ
                     LQAVAKEGDLFIVFSPVGNEECILQAIHTAKNQHLDLDIIALTGSNNDHTLGLLDESD
                     IEISAPSTNQLRVIESHHFYVNLLCELIDHLLFS"
     gene            11748..12338
                     /gene="yraP"
                     /locus_tag="PWX02_000486"
     CDS             11748..12338
                     /gene="yraP"
                     /locus_tag="PWX02_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HDL1728528.1"
                     /translation="MNKLANKLLKGSLLVLGFISLQGCITTAVVTSVAVATKVATDPR
                     TTGTQVDDEILEEKVAYNINKDEQIKQEARINVVAYNGKVLLIGQAPSMDVVENAKNL
                     AAGAEGVTEIYNEIRQGEKIGFGQITQDSWITTQVKSKLLVNGEVKATEVKVVTENGE
                     VFLMGKVSQNQADAAAEAARNVGGVTKVVKVFSYAQ"
     gene            12628..12819
                     /locus_tag="PWX02_000487"
     CDS             12628..12819
                     /locus_tag="PWX02_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728529.1"
                     /translation="MLFKEQGYDEFLAEQIKAGQEAIARGETFTKEQINNELNHLFAD
                     MQKRQQLRENENLFEVNYA"
     gene            12812..13099
                     /locus_tag="PWX02_000488"
     CDS             12812..13099
                     /locus_tag="PWX02_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL1728530.1"
                     /translation="MPKSWELSLTARSNVIEILQSVEDYTGHWLSVVSLWEQLQNKLD
                     LIAFMPTIGRRREDGTLETFCRNYRIVYEIHLEKVYIITIIHSRRLYPRPS"
     gene            complement(13246..14388)
                     /gene="argE"
                     /locus_tag="PWX02_000489"
     CDS             complement(13246..14388)
                     /gene="argE"
                     /locus_tag="PWX02_000489"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016533842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HDL1728531.1"
                     /translation="MKQISFIQRYAQLIELPTISSLVAEEDLSNRRLIELLATWLADF
                     GFKTEILAVEGSHNKYNLLATYGEGEGGLLLAGHTDTVPFDEGKWRFNPFQLTEKAGK
                     LYGLGTADMKGFFAFVVEVVSQLDLTQIKKPIRILATADEETTMLGARTFAQHSHIRP
                     DCAIIGEPTSLKPIRAHKGHIGESVRITGRSGHSSDPAKGINAIELMHQSIGYLMKMR
                     DELREKYHNPLFQVTHPTMNFGNIHGGDAINRICACCELQFDMRPLPNMAVQDLYAMV
                     NEHLKPMLEQYGDLIEIRHLHDGIPGYECEHSAQIVQVVEKLLGEKCDAVNYCTEAPF
                     IQQLCPTLVLGPGSIEQAHQPDEFLEMKYIEPTKELLTKLIYHFCG"
     gene            complement(14392..15183)
                     /locus_tag="PWX02_000490"
     CDS             complement(14392..15183)
                     /locus_tag="PWX02_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL1728532.1"
                     /translation="MKTEWIKHLIQQLTTLSKAIENYFTSGSFGVKFYPFLMHPCFRK
                     SCAFLIFIIGMVSSFSGLFYLVAEHITILFSERSLTTYFHHSTLELMIPVGTMIDEKI
                     SELSPLSVVMRSMFVLQAVVFFFIYAIGITHIAHNKLRVIGLLLSLCFAIGCSLVAGI
                     QPSSRGEFGIYNLGASITFLIGNLTLIIAGFDLYHPSMQFFKRFSIGAGVIGAICILI
                     TIFLPTLFSPLIERAGIYTIMLWEILAGFAIIKRLLKLPSFRQQG"
     gene            complement(15199..15804)
                     /locus_tag="PWX02_000491"
     CDS             complement(15199..15804)
                     /locus_tag="PWX02_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HDL1728533.1"
                     /translation="MLDQTIIDQYDALIFDMDGTLIDTMPSHAKAWEKVGEVLGYPIN
                     PKPMYELSGSTTFVIAREIMQRSNIPEHYFEQVVQLKREFGIEMVLANATLLPAFEII
                     KANVGKKPMAIGTGSHRAMVELLDQKFNLRQYVSVIVDSDDVSNHKPAPETFLKCAEK
                     LGIAPHRCLVFEDADLGVQAALNGNMNVFDVRTNTITHSTR"
     gene            complement(15825..16733)
                     /gene="rdgC"
                     /locus_tag="PWX02_000492"
     CDS             complement(15825..16733)
                     /gene="rdgC"
                     /locus_tag="PWX02_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HDL1728534.1"
                     /translation="MVWFKNVMIYKLTGELALESNSLNELQQETRFTPCSQHDMSKFG
                     WATPLTGSDLLHFSQANQFLLISHKEDKLLPANVVKAETEQRIAELEEKEARKLKKTE
                     KQAVKDQIVSALLPRAFSKHQFTALWLDLDNQLVYVDAASSKRAEDTLALLRKTLGSL
                     PVVPISFTLPPAEVMTNWIAKGHTPSWLNLLEEAELKSFDTDSIIRCKRQDLETEEIA
                     RHLQAGKFITKLAIEWEGHFNCVLNEDATLSRLKFADEVREKNDDILKEDVAQRFDAD
                     FLLMTEELKLFTEKMIAEFGGIKSRI"
     gene            16810..17628
                     /locus_tag="PWX02_000493"
     CDS             16810..17628
                     /locus_tag="PWX02_000493"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDL1728535.1"
                     /translation="MNEKKLAFIGTGNMAYAIIQGLLKSGYSAEQIIACNKSNLARRE
                     ELQAVGIATNFANREAVEQADVIVLAVKPQMMAQVCAEFADVDFSNKWVISVAAGISV
                     QRLETLLPSAKNIIRTMPNTPSLISEGMTGLFAKKSVNPTACQFAEQLMSAVGKCYWV
                     ETEEQINHIIAITGSSPAYFFRFMEAMQQSAVQMGFSEQDARTLVQSVALGATKLVEA
                     NPTTPLSTLRENVTSKGGTTAKALEVFEQHDLAKTVDDAMWAAIHRAREMEQSL"
     gene            17625..18779
                     /locus_tag="PWX02_000494"
     CDS             17625..18779
                     /locus_tag="PWX02_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HDL1728536.1"
                     /translation="MMKVSPFQWAAFNYFGFFCAYGVLLPFLPVWLKHHGYSTEMIGL
                     FAAVGYLFRFAGSMLASQRVKTVSQLIPTARSLTWLNIAAAVALLFSGDFIWLLFPVL
                     MLFQMFNSGAMPIADSIASIWQHQIGLDYGRARLFGSMAFVVGSLSAGYLIGLLGENS
                     VVWIIIGFLVFLGIGQLATPKVGFEEQGNKANTSSVTYRQLLKEPETLKMLIAVSLIS
                     ASHAAYYTYSTIHWSNASISTAMTSLFWGLAVVAEILFFLVSKRLFKSWKISHLMILA
                     AVFAVIRWAIMGSTTEIVLIIVMQMLHSITFGAAHIAMIRYISMQSAERVTKLQGLYF
                     GLASCAFMAVFTFIAGMVYQHSPSLTFWLMAIFTLPAIFIVPKRFEVQIN"
     gene            complement(18839..19052)
                     /locus_tag="PWX02_000495"
                     /pseudo
     CDS             complement(18839..19052)
                     /locus_tag="PWX02_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034825.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
     gene            19380..19913
                     /gene="proQ"
                     /locus_tag="PWX02_000496"
     CDS             19380..19913
                     /gene="proQ"
                     /locus_tag="PWX02_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HDL1728537.1"
                     /translation="MSEQIEQKVEQTQNKTNPSTKEVIAYLAEKFPLCFTLEGEVKPL
                     KVGLFQELAEALKDDEKISKTLLRQVLRNYTNSWRYLAASQANVARVGLQGEEVGVVT
                     EDEATHAAEALAAAKVVAERKAQERKAQRKEYFKQKAREENAKKRAEFKAKKAIDNLP
                     KASEEKLAALADKFGRK"
     gene            19999..22011
                     /gene="prc"
                     /locus_tag="PWX02_000497"
     CDS             19999..22011
                     /gene="prc"
                     /locus_tag="PWX02_000497"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HDL1728538.1"
                     /translation="MKLTRLNKIFALALGLMIGNAFAVQPDIKESAIVMPKPNEQHSL
                     STKRVTARLTQSHYHKFQLNDEFAGKVFNRYVDWLDPSHNTFLQADIDEMRSKYATTL
                     DDDLYAGKLDAAFAMYELMSKRRYERYSYALSLLDKEPDLKGNDEIEIDREKAAFPKS
                     VEEANTLWEQRVKNDIINLYLKDKKWLEIKKTLTKRYNFAIKRLTQVKADDILQTYLN
                     SFSREIDPHTSYLSPRAAKAFQESMNLSLEGIGATLTQEDDVTSIRALVPGAPAAKSK
                     KLVVGDKIVGVGQGERGEIEDVIGWRLDNVVDKIKGKKGTKVRLEIEPEKGGKTKIIT
                     LVRDKVRIEDSAAKLTVEKVDGKNVGVIKIPTFYIGLTSDVRKLLTQIEKKNVEGLII
                     DLRENGGGSLTEVIELTGLFIKDGPVVQVQDAFNRIKVHEDPESKSVYDGKVMVMINR
                     HSASASEIFAAAMQDYNRAIIVGQQTFGKGTVQQSRSLNFVYDLDQEPLGFIQYTIQK
                     FYRINGGSTQLKGVEADIKFPEIINAAKTGESFEDNALPWDKIPAANYKEAGNARNAV
                     AALTAKHQERIAKNPEFIVLNENIAIRKERDERKFLSLNLEERLKENKADESKRLKDI
                     NARFAREGKKALKDIDDLPKDYEAPDFFLKEAEQMMLDWSEFDKKS"
     gene            22147..23634
                     /locus_tag="PWX02_000498"
     CDS             22147..23634
                     /locus_tag="PWX02_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HDL1728539.1"
                     /translation="MALAETATVAQVASEPVVQDNILPQLSFAAQQARAAELVKQADY
                     ALEQEQIRQEQARKFAEAEARIKQEIGNNALLLSQATTKVYLDSEFATMWNDKAAEKA
                     FLKEYALFAVSGVSSKSAKALQQILNQPEGLGRDILLTDGFLDYLYYNKNVFKNANNW
                     LYNLGSYVPKSPSSEQIFQWTNAVREGDSGQFVTNLVPRNHLYRDTAQRVLAMAGGSS
                     APKAKKGKAVTEVAGGGNGASFYKLALNAQRLRIIPSFNNGIFVNIPSYQLYYFRDGN
                     LALQSKVIVGREDRRTPVMDSKLSNVVVNPPWNVPTSIMTKDIIPKLARDPGYADRAS
                     FEILDSSGNKINPRSVNWSQYLNAKSSPYRIRQKAGDDSALGRYKFNMPSSDAIYLHD
                     TPNRGLFGKTGRALSSGCVRVEKSDELATILLKEAGWGSDKKNQVLGSKKTTSAQIRS
                     DNPVYLYYVTAWVEGGKVQTLPDIYNFDAKIPQHSVNWGKLKSII"
     gene            23804..24358
                     /locus_tag="PWX02_000499"
     CDS             23804..24358
                     /locus_tag="PWX02_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HDL1728540.1"
                     /translation="MKQINVQRRKWLSLGGLVLGASLLPGQVMAALSTPAPAALRFRN
                     VNTGDTYTAKFGAGGLNKTDLSQLNYLMRDRHINQVKAIDPKLFVKLNQLQRRLGFHN
                     AEILVLSGYRSAQTNARLRRRSRGVASHSYHILGQAVDFQVSGVPLYKVKQAAESLNN
                     GGVGYYPRSNFVHVDTGPVRTWRG"
     gene            24375..25004
                     /locus_tag="PWX02_000500"
     CDS             24375..25004
                     /locus_tag="PWX02_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL1728541.1"
                     /translation="MFNLQIIPVSPFQQNCTVIWDENKNAAIIDGGDNAEGIIQFVES
                     QQLKVQKLLITHGHLDHIMAVEKLREHFQVEVFGSHLADKPLFERLPEMCQQFGLPPV
                     NGFLPDHWLEEGDRVEVGELSYTVRHLPGHAPGHIGFFDFDNRIAFSGDVLFQNSIGR
                     TDLYLGNHSQLLETIREKMFDLDDDFIIIPGHGPHTTIGREKQTNPFLK"
     gene            complement(25039..>25231)
                     /locus_tag="PWX02_000501"
     CDS             complement(25039..>25231)
                     /locus_tag="PWX02_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HDL1728542.1"
                     /translation="FPLNLTYKLFWYNKDGVTQSHNGISDSIGWQHLWLQPKQKQTIA
                     LIKPTEESHNYRLYLRGVK"
BASE COUNT         7285 a         4910 c         5564 g         7472 t
ORIGIN      
        1 agctatcgaa acacgctaaa aataaagcct ggattataat ccaggcttta tttttatatc
       61 tttaaagaat tattttacta aatcttttaa tgctttacct gaaacgaatg caggtacttt
      121 agctgctgca attttgatct cttcaccagt ttttgggtta cgaccagtac gtgctttacg
      181 ctcatttact ttaaaagtac cgaagccgat taattgaaca gcgtcgcctg cttttaagct
      241 tgcagagatt gcatcaagag ttgcttctaa tgctgcttta gcatctttct tggttaagtt
      301 tgcaccagcg gcgatagcat cgattaactc agttttgttc atagttttat cctctaagat
      361 atgtgttttt aataaatgac gtaaaacgtc tgagaagtta ttgaaacctt gcgatagctt
      421 aattcaaagt taagatttct caaagtatta ttaagcaaga ttttgcaaat atcaatgcta
      481 ttggttaata tttaacccga tattactaat cccgctgctt ctgatttcat ctttaagttc
      541 aaaaatgaga tcaagatcac gtttttcaca ttcgtttagc aagcggtata tttgccactg
      601 tacgtccagt tcttcttgaa tttcttcaga attctttagc tcttgctcgt tcagttcttt
      661 atcttgttgc atttccaaat agctggcaac tgtgcctaat gaaatttggc tgatgcggat
      721 tgcttgttca agggttgaac cagcgataat actgtgaaga agttcagaaa gggcttcgca
      781 agcttcctgt gcaggtaaga caccaaaaaa cgcatattcg ttcacatcag gaatgatttc
      841 ttctaatttt tccagttggt tatcgaaatt aatttttgcc ccttttatgg ttaaatattc
      901 ccagactaaa tttaaaatat tttggaatac cttagctttt tcaggctgtt cggttacttc
      961 acaaaaaagc tgatagttgg ggtacattct ttcgcatagg cacgccataa atgtggtgtt
     1021 ttgccaaggc tcgaaatgtt ccatgcgttt atgaatcggg tttctcattg tatttccttc
     1081 tttatttttc agagagaagg gaacgatttt gcaaaattct gtataaaaat gaccgcttgc
     1141 ccctctccct gatttttctt ctgaacaaag aaaaatctgt ccctctccca caaggagaga
     1201 gggcatttta tccattattt atgatactgc ttcgagtagt tgtgaatcgc atcaacaaac
     1261 actttcgggc tttccaccgg cacatctaaa tgaatgccgt gtccaagatt aaagacgtgt
     1321 ccgctgccgt tgccgaagcc ggcaagaatg ttttttactt cttgttcgat tcgttcaggg
     1381 cttgcataca gcacgcttgg atccatattg ccttgcaacg caactttgtc gccaatacgg
     1441 cggcgagcat cggcaatatc gactgtccaa tctagcccaa cggcatcgca gcccgtttct
     1501 gcaatcgctt ctaaccataa gcctccgcct ttggtgaata gggtaacagg cactttacgt
     1561 ccttcatttt ctcgaattaa gccgtccaca attttgtgca tatagtgtaa tgagaaggct
     1621 ttgtaatcct cgtgagccaa tacaccgccc caagtgtcga ataccatcac cgcttgagca
     1681 ccggctttga tttgggcatt taagtataag atgacactgt cagctaactt gtccagcaat
     1741 ttgtgtagaa tgtgaggctc agcgtaaagc atttttttga ttttggtaaa tgctttgctt
     1801 gaaccacctt ccaccatata ggtggcgagt gtccaagggc tgccagaaaa accaattagt
     1861 ggcacttcgc ctttcaattc tcggcgaatg gtacgcaccg cattcattac atattgcaac
     1921 tcgtcttccg gatcaggaat cggtagctga ttgacatcat tgaggcttgc caccgggcgg
     1981 gcaaatttag ggccttcacc tgcaccaaaa cttaaaccta atcccattgc atcaggcaca
     2041 gttaaaatat cggaaaatag gatagctgca tcgagttcat aacggcgtag cggttgtaag
     2101 gtgacttcac acgctaaatc agcgttacgg cagagtgaca taaaatcgcc ggcttctgct
     2161 cgggtggctt tatattccgg caaataacgc ccggcttgac gcatcatcca gaccggggtc
     2221 atatccacag gttcacgcaa taacgctttt aaatagcgat cgttttttaa ttctgacatt
     2281 ggtttttcct ttgattttgg taggggcgaa atatttttcg ccccgcataa tatatttgca
     2341 aaatagagat gaaattgaac cgcttgtatt tattcattag tgtaatggtg gcttttcttc
     2401 ggttttcggc acgcctaaag caaaatggct gtgcaagaat aaggctaaaa ctcgcagata
     2461 ttccaacact tcttcgccag cctccagtag ttcctcgtta ttatcatcgt cactgtagcc
     2521 tagtttggcg atttcatcta aatcatcgat tgcctcgcca acctcatcgg tttcttgttg
     2581 taatttaggt tgagcaacgc caagtccaag taagaaactg tttgtccatt cggagatacc
     2641 atcagctaag gcaaaaccat cttcttcgtt ttgtggcaac caaaggctaa acagcgtgtt
     2701 ggcttctgaa aagctttcac ttaattgctg ataaaaatcg gttaaagtgt ttaacggctc
     2761 gatggaaaat gcgtgtccgt cattggtaaa ttggtaggtg agggttttcc aagactcatc
     2821 ttgaataccg ccggcaatta aaccgcataa aaaaccgtga aactctgccg gtgtatcatc
     2881 aatttgtaat tgtgatacca gttgtttaaa ttgttgggaa tctgtgatag ccataatggg
     2941 attatttgtg attttgctaa agaatggcat aatatagcaa aaattttgcg attaagtgag
     3001 gatttcgtat ttatgtcggc aaataatatc gaactacaga tttttgggca ggttttacgc
     3061 ttgcaatgcc ctgttgaaca gcaagagcat ttactggctt cagcacagcg tttggaagag
     3121 cgtgtggcgg tattgaaaga gcagtccggc attattcagc ttgaaaaagt gttagcgatt
     3181 gtggcactga atttaaatta tgaactggag caagcccaac gccaaaatgc agaaaataaa
     3241 aatgtgttgg tggcttgtat tgagcagtta gataattcgc ttggcaaatt tcaatccaat
     3301 taggtaattt ttaatcgaat tgattaggaa tagagccaaa aagattgaga atttgcattt
     3361 tgtgggatta aatactagga aaaatcatca aattccgtta ctataagtct tgcctggggt
     3421 gtgcgttacg agttacgtcc ctgagccgat aatttgaaac tttgagttgg acatctaacg
     3481 ctggtgagca agcttaccct aatttaatta ggagtaagaa gcctaaaagc agttaagttt
     3541 gactcccttg aaccgactgg ttcaaggacc attactggta gcggcatctc gggtctttat
     3601 atgaatcaat catctttaac cgatcttcgt agccaattac ggcaatcaat gcggcttaaa
     3661 cggcaaagtt taactgccgg tcagcaaact caagcagccg aatccattat tcctcaagct
     3721 ctttcattga ttgaatctta ccaagcctca catattgcct tttatttgcc ttttaacggt
     3781 gaaatttctc ctattccttt aatggaacaa ttacttcaac agggcaaaaa gctgtatctg
     3841 ccgctgttgc acccttttac aagcggtcaa ttattgtttg taaattacaa ttgcaaaact
     3901 attctaaaat ttaaccgctt ggggattcaa gagccgatat tagatgttag aaatatcctt
     3961 ccgttgcaag aattagagat gatttttacc ccacttgttg cttgtgataa agccggaaat
     4021 cggctgggaa tgggaggtgg cttttatgat agaacccttg ctcaagcacc tcatctgatt
     4081 agtgtcggtt tggcacacga atgtcagcaa gtcgaacaat taccgattga gagttgggat
     4141 atgccgttag atcatattat tttgggagca gccaaatgaa tgcaaagaaa gaaaaggaat
     4201 cgagtggaaa tcaaagttta attcgtggct taacactatt ggaattgtta gcgaaattcc
     4261 caaacggctg tccgcttgcc catttagccg agctttcggg gctgaacaaa agcacggttc
     4321 accgtctgct acaaggctta caacaagagg gctacgtccg ccctgcaccg actgcgggca
     4381 gttatcgttt aaccaccaaa tgtttggcaa tcgggcagcg agccttaagt tcgattaaca
     4441 ttttgcatat tgccgcccca catttagaag ccctaaattt aaccttaggc gagacggtaa
     4501 attttgcgat gcgggaaaac gatcacgcta ttttaatcaa taaattagaa gctaccaccg
     4561 gaatgatgcg tacccgagcc tatatcgggc aacaaatgca actctattgc tcgggaatgg
     4621 gaaaactgtt tcttgcttat ggttcggcag attacattcc acaatattgg cagactaaat
     4681 cggacatcat tcaaaaactg acggtcaata ccatcaccac gattgacgga atgcaaaccg
     4741 agctggaaac cattcgccgt caaggttttg caatggataa cgaagaaaat gaattagggg
     4801 tttcttgtat tgcttgcccg atttttgatc accaacaaaa agtccattat gcgatttctg
     4861 tttcgctttc aactgctcgt ttaaatcagc tggggcggga aaatctacta ccggctattc
     4921 aagaaacagc agaaaaaatt tcagctgagc tgggcggacg gtaattttgt gttgccattt
     4981 tgattatttc aggtaatctt ttacccttta ctcactttaa tctttataca ataagaaatc
     5041 ttgaggattc attaataatg tttggtcttg atccaacaac aattatgttt gttgtctata
     5101 ttctaggaat gattgccatc ggctttattg cctatcgtgt taccaataac ctttcagact
     5161 atattttagg cggtcgccgt ttaggtagct ttgtgaccgc actttctgcc ggtgcatcag
     5221 atatgtccgg ctggttgctg atgggattac ccggagccgt ttatgcaggc ggtttgatag
     5281 aaggctggat tgctatcggt ttaactatcg gtgcttattt aaactggaaa ttagtcgcag
     5341 gcagattacg ctctcacacg gaacacagtg gcgatgcctt aaccttaccc gaatacttcc
     5401 acaatcgttt tggcgataaa acccgtttaa ttaagatttt atcagcggtc atttttttac
     5461 tcttttttgc aatttactgt gcatcgggcg tcgtggccgg tgcgagagta tttgaaaact
     5521 tatttggctt accttaccac caagcaattt ggtatggggc tgttgctacc attctttaca
     5581 cctttattgg tggtttctta gcagtaagtt ggacggatac tattcaagct tcattaatgg
     5641 tttttgccct gattcttacc ccgttttttg tgatgtataa cttaggtggt tttgatgaaa
     5701 tgcaagcctt aattgatcgt gcaggcgaga tggcacaaaa agactacaac gacattttca
     5761 ccggcaccag cgtattaggc attatcagct taatggcgtg ggggctgggt tatttcggac
     5821 aaccgcatat tgtggtgcgt tttatggcgg cagaaagtgt gaagtctctt gaaaatgcac
     5881 gccgtatcag tatgacttgg atggtgattt gcttattagg tgcaatcggt attggttatt
     5941 ttggtatggc atatttcttc ggacacccgg agcaagtcgg caatatcagc aaaaatccgg
     6001 aacaaatttt cattgaatta gctaaattgt tatttaaccc ttgggtagcc ggcattttat
     6061 tatctgccat tcttgctgcg gtaatgagta ccctttccgc tcaattactg atttgttcaa
     6121 gtgcgattac cgaagattta tacaaaggca ttttacgccc tcaagcctcc gataaagaac
     6181 tggtttggtt aagccgcttg atggtgcttg cggtggcagc attagctatt tatattgccc
     6241 aagacccgga cagcaaagta tttggcttag tgcgtaatgc gtgggcaggt ttcggctcgg
     6301 catttgggcc tgtggtattg ctttcactat tctggaaacg tatgaacggt tttggggcga
     6361 ttgccggaat gctgaccggt gcgttaatcg tgcatttttg gggcgatatt acacttaagc
     6421 tcgccttacc ggaaatttac tcgatgatcc cgggctttat tgctgctacc ctaatgatca
     6481 ttgtggtatc acttatcacg ccaatgccaa gcaaagccgt attagacacc tttgaccgtg
     6541 ctaataaggc ctatttggca gaagtgaaat aataaaattt ggcttaaggt ctccttaagc
     6601 cattttttta caaaaaattt atcaaaaatt attcacaatc cttgtaagga ttgttcacag
     6661 cttcgctccc gttggcagag ccaacgttca aaaagcgatg ctttttgtga ccgattgtaa
     6721 gctatgacta acctcctact cgctattatt tatctctctt ttatcagcct tgggttgcct
     6781 gatgcaattt taggctcggc gtggccgtca atgcaccaac agcttgctgt tccgctttcc
     6841 tatgccggtg tggtctcgat gattatgtcg attggcacta ttacctctag cttaatgtcg
     6901 gacaaactga cctaccatct cggcacaggc aaagtaatgg tcatcagcgt tgccttaacg
     6961 gctttagcat tgtggggctt ttcaatcagt gatgctttct ggatcttatg tttatgggca
     7021 attccttacg gcttaggggc tggagcggtt gatgcggcgt tgaataacta tgtagcagtt
     7081 cactacgcaa gccgccatat gagctggctg cattgtatgt ggggcgttgg agcttctatc
     7141 gggccgcaca ttttaggcta cgccttattg caagggcgag gctgggaggc aggctatttc
     7201 accatttcaa tgttacaaat cgggctaacg ctaatgctgt tttgctcact gcctttttgg
     7261 aaattacgca agcagattac cgctgaaatc aaccattcgg caaaaccgat gagcttaaaa
     7321 gaaacgctaa aaattccggg cgtgaaatat ggcattttag ctttttttgg ctattgtggc
     7381 gtagaacaaa ccgccggact gtgggcgagt agctattttg taatggttaa agatgtcggc
     7441 ttagaacaag cggctgcgtt cggcagtatt ttctttatcg gcgttaccgt tggcagaggc
     7501 gtaagtggct tcttgacgct taaatttagc aacagacaaa tgattaaact tggttcagcc
     7561 ttgatcttag tcggcattgc gatactgtgc ttgccgttag gcaaatatgt tacgctggca
     7621 agtttaatta ttatcgggct aggttgtgcc ccgattttcc cgtgcttaat tcattcgacc
     7681 cctgcgtttt tcggcgagga caaatctcaa tcggtgatcg gcgtgcaaat ggcaagtgcc
     7741 tatatcggcg tcctgttatt gccaccgatt ttcggcttta ttgctaatgc tatcagcatt
     7801 caattattac cgctttactt gctgttgatg tttgcgatta tggcgtatgc ttatcggaat
     7861 ttagtgcgag aaacttcccc ctctccctga tttttcttct gaacgaagaa gaatctgtcc
     7921 ctctcccacg agtggagagg ggtcgacaag cgaataattt agctctctcc ccttatggga
     7981 caaatgttta catttgaacg ttggctttgc caacggctcc gaaggagcga gcgtagcgag
     8041 tattgagacc gggaaatgtt tcgttcagaa acatttttca gagagagggg agtgccaatc
     8101 tgcaaaaaac tcacaaaatc tgaccgcttg tatgttactc aaaatgctcc aatccatatt
     8161 gatacagggc atttttctta tagccgaagg tttctgccac aatggcagcg gcttttttaa
     8221 gcgggagttc ttggcagagt aatgtcagta atttgatcgc ttggctggaa aaggcttcct
     8281 cactactttg cggtttacct tcaaccacca gaacaatttc gcctttaatt cggttgctat
     8341 cctcatttag ccattcaatt aagttaccca gttgatctcc ataaatggtt tcccacgttt
     8401 tcgtgatttc acgcgccatt accacatagc gatcttcgcc aaaaacagtc tgcatatcgg
     8461 caagagtgtc taaaatacgg tgggtggatt cataaaaaat cagtgtgcga ggctcgtttt
     8521 caagctcggt aagtttatcg caacgggatt ttgtttttgc cggtaagaac ccttcaaaac
     8581 aaaaacggtc tgaagctatg cctgaagcac ataaggcagt aacagcggca caagccccgg
     8641 ggatcggcac aacttttacc ccggcttggc ggcaatgacg gacaagatga aagcccgggt
     8701 cgctaatcag tggcgttccg gcatcggaaa ttaacgcaat attttcccct tttgctaatt
     8761 tttcgactaa aacgaccgct ttctgctgtt cgttatggtc gtgcaaggca aaaaacggtt
     8821 ttttaatacc gtaatggctc agcaataaac cgctgtggcg ggtgtcttcg gcggcgacta
     8881 aatcgacaga ggaaagggta tctaacgctc gttgggtaat atcgcctaaa ttgccgatgg
     8941 gggtggcaac aatatataaa attccgtaat ttgggttgat ttcagatgaa ttttgtctgt
     9001 ttttcatttg cttttagctc gtttgataag taagatttca cattaaattt agcacattat
     9061 attctattga ggatctgaca atggcaacta ttttaaacca tacaatgaaa aaagcattgg
     9121 tgccaacggc gattgcatta ttcatttctg cctgtacatc ggtaaatcct gtcaccgaat
     9181 ccattaaaaa tgaagcctat tcaagctcgg aattctatat taataaagca gatcaaacca
     9241 aagaagcgga agataaaatt tcgtatcaac ttttagctgt acgcaaatta attgatgaaa
     9301 ataaggaata tgaggctcaa aataccttta gtgaaattct gaccgcagag atgaacgagg
     9361 tgcaaaagtt agagtatgcc ttggtttccg cccagcttgc cgccttacaa gggaaaaacg
     9421 agcaagcaac aagccaatta aaagccattc aagatccgct attaagttcg gcacagcgtt
     9481 tgcgttatat tcaaacccaa gcccgtattg ctgccaatca gaaagatgtg attggcattg
     9541 tgcgtgcaag atcgcaactt aacaatttct ataaaatgaa ccgtgaacgc caagaaaata
     9601 acgatattat ttggcaaacc ttgcgtgatg caaatcgagg aatgctggaa aaaactgtgc
     9661 ctgaagcggg tgagatggag cttgctggtt ggttggcatt aattaatatc tataatcaaa
     9721 atgtgacgac accggcacaa atgccacaag caattaataa ttggaaagcc caatacccga
     9781 gccatagtgc ggttgccgta atgccaacgg aattacaagg ggtgagtaat ttccaacaaa
     9841 cccagttaaa cagtgtcgca ttgctattgc ctctaagtgg tgatgcaaaa attttgggtg
     9901 atattatcaa gcgtggtttt aatgatgcta aggctgatga ctcaaccgca gtgcaaactt
     9961 ttgataccga ttcatctgat gttaattcct taatttcaca ggcaaaacag caaggggcaa
    10021 atgtgattgt cgggccattg ctcaaatctc gtgttgatga aatgctactt agcccagaaa
    10081 tccaaaacgt gaatattttg gcactgaatg ccactgaaaa tgtgcgtaat attgctcagg
    10141 tgtgttacta cggtttatct cctgaatctg aggcacaatc aggggcggaa aaaatttatc
    10201 aagacggtaa tagtgtcgct atcgttgcgg cccctcaaga tgactacggc aaccgctcgg
    10261 cagaagcctt tgccaaacgc tggcgtcagc ttaccaatag cgatgctgat gtgcgttact
    10321 acaatcaacc gcttgatgcc gttgccgcta ttcaaaatgc aggtgtgagc aaagcagctt
    10381 tatatatttt ggggaatgcc gagcaagtat tagaaattaa acaaggtatt gatagctcaa
    10441 ccttaaaaga tcgtttagcg atctatacct catctcgcag taattcgcca aataacggca
    10501 ttgattttta cacttcaatg gaaggggtaa aatttagtga agtgccactg ttagctgatc
    10561 aaagttctag tgaatataaa aaagccgaga atttagccaa tagcgatttc tcaatgatgc
    10621 gtttatacgc aatgggggct gatgcgtggt cattggcaac caaattcaat gaattccgcc
    10681 atattccggg ctacaaaatt tcaggtttaa ccggtgagtt aaaagccgga caacattgta
    10741 atattgaacg tagtttatct tggatgcaat atcgcaatgg agctattcaa caagcttatt
    10801 aataaacggg aacaaggctc gttttatgaa caaaaagccc gagtgttttt agaacagcaa
    10861 gggcttcgtt ttgttgccgc taaccaagca tttaaaggtg gcgaactgga tctgattatg
    10921 caagacggac aaaccgttgt gtttgtagaa gttcgccagc gtaaaagcaa ccgcttcggt
    10981 tcagccgttg agagtattga ttaccgcaag caacaaaaat ggcaaaatgc cgctaatatg
    11041 tggttgctta aacaaaacca aagtttagaa actgcaaatt gccgttttga tgtggttgct
    11101 tttgaaggaa accaaacgcc tgtgtggatt cctaactttt taggttagaa agaatgttaa
    11161 ataaaatcca agatagattt accgaaagta ttcaaaccca aatttccgcc gctgagctat
    11221 tgccggctaa attaagtgaa gcggcaaacc gcattgttgc ctgtttgttg cgtggcgata
    11281 aagttatcgt ctgcggacac ggtcgctctt acggtaatgc cgaattattg gtcagccact
    11341 tacttcaccg ttatgaactg gcaagaccga gttttgctgc cgtacaactg caattaagcg
    11401 gtgtattggc aggagttgtg gcacaagata acgagttaga ggctgtttat aaaaaacagt
    11461 tgcaggcggt tgcaaaagag ggtgatctct tcattgtttt ttcgcctgtg ggtaatgaag
    11521 aatgtatatt gcaagctatt catacggcga aaaatcaaca tttagattta gatattattg
    11581 ctttaacagg cagtaataac gaccacacac tcggcttatt agatgaaagt gatattgaaa
    11641 tttcagcccc ttccaccaat caactgaggg tgattgaaag ccatcatttt tatgtgaatt
    11701 tattatgtga acttatcgac catttactat tttcgtaagg agagattatg aacaaattag
    11761 caaataaact attaaagggc agcctattag tattaggttt tatctcttta caaggttgta
    11821 tcaccacggc ggtggtgact tctgtggcgg ttgcaactaa agtggcgaca gacccacgca
    11881 ccaccggcac gcaagttgat gatgaaattt tagaagaaaa agtcgcttac aacattaata
    11941 aagacgagca aatcaaacaa gaagcccgaa ttaacgtggt ggcttacaac ggcaaagtgt
    12001 tattaatcgg gcaagcacca agtatggacg tagtggaaaa tgcgaaaaac ctcgctgctg
    12061 gggcggaggg cgtgacggaa atttacaacg aaatccgcca aggcgaaaaa atcggctttg
    12121 gacagattac acaagacagc tggattacca cccaagtgaa atccaaactg ttagtaaatg
    12181 gcgaggtaaa agccaccgaa gtgaaagtcg tcaccgaaaa cggcgaagtg tttttaatgg
    12241 gcaaagtcag ccaaaaccaa gctgatgccg ccgccgaagc cgctcgcaat gtaggtggag
    12301 taaccaaagt ggtgaaagta tttagttatg cgcagtaatt gataagctca attagtttat
    12361 atttttgtaa ctcacaagcg gtctaaattt gtcatttttt tgcaaattta gaccgcttgt
    12421 ttgatataag aagatgacct tagataggaa gataaaatct atgagtggat gatcgtaatg
    12481 atgattttta attcatttga ctcgaaaaat atgcacaccc tatcaacata aggattccct
    12541 tgactcttca ttgctttgag cgtagattta aacttaaact atgtcttgaa catagtggaa
    12601 tcaagacaca aatcaaagga gcagaatatg ctattcaaag aacaaggata tgatgagttt
    12661 cttgcagagc aaattaaagc tggtcaagaa gcaatagctc gaggagaaac ctttaccaaa
    12721 gaacaaatca ataacgaatt aaatcattta tttgcggata tgcagaaacg tcagcaatta
    12781 cgagaaaatg aaaatttgtt tgaggttaat tatgcctaaa tcttgggagc tgtcacttac
    12841 cgcaaggtct aatgtcattg agattttaca gtcggtagaa gattatacag ggcattggtt
    12901 gagcgtagtt tcactttggg aacaattaca aaataaactc gatctcattg catttatgcc
    12961 tacaataggg cgacgtagag aagatggcac attagaaaca ttctgccgca attatcgtat
    13021 tgtctatgag atacacctag aaaaagtata tattattact ataattcatt ctcgccgttt
    13081 gtatccaaga cctagctaga gattaatacc tatctaacag aatctcctta aatcaatgat
    13141 gaagtatgga agtttatagg tcactcaata ctaaatatca agcggtcatt tttgccgatt
    13201 cttttgcaaa aagtatggcg aaaatgagcg cttgtttctg ttgatttatc cacaaaaatg
    13261 ataaatcaat ttagtcaata actcttttgt cggttcgata tatttcattt ccaaaaactc
    13321 atcgggctgg tgggcttgtt caatggagcc ggggcctagt accagcgttg ggcagagttg
    13381 ttgaatgaat ggggcttcag tgcagtaatt gacggcatcg catttttcgc ccagtagctt
    13441 ttcaaccact tgcacgattt gtgccgagtg ttcgcattcg tagcccggaa ttccatcgtg
    13501 taaatggcgg atttcaatta aatcgccata ttgttccagc atcggtttta aatgctcgtt
    13561 taccattgca tacaaatctt gcaccgccat attcggtagt ggtcgcatat caaattgcaa
    13621 ttcgcaacag gcacaaatgc ggttaatggc atcgccgccg tgaatattgc cgaagttcat
    13681 tgtcgggtgg gtgacttgga acagcggatt gtggtatttc tctcgcagtt catcacgcat
    13741 tttcattaaa taaccgatag attggtgcat taattcaatc gcattaatgc ctttggcggg
    13801 gtcgctggag tgtccgcttc tgccggtaat gcgaaccgat tcgccaatat gccctttgtg
    13861 ggctcgaatc ggtttgagcg aggtcggctc gccaataatc gcacaatcgg ggcgaatgtg
    13921 gctgtgctga gcaaaggttc ttgcccccag cattgtggtt tcttcatcag cggtggcaag
    13981 aatgcgaatc ggctttttga tttgggttag gtcgagttgg ctcacaacct ccaccacaaa
    14041 ggcgaaaaag cctttcatat ctgccgtgcc taagccgtag agtttgcctg ctttttcggt
    14101 taattggaat ggattgaagc gccattttcc ttcatcaaac ggcacggtgt cggtatgccc
    14161 tgccaataat aaaccgccct caccctcgcc ataggtcgca agtaaattgt atttattatg
    14221 gctaccttct accgctaaaa tctcggtttt aaagccgaaa tctgccagcc aagtggcgag
    14281 caattcaatc aaacggcggt tcgataaatc ttcttcggca acaaggctgg aaatggtcgg
    14341 tagctcaatc agttgagcat agcgttgaat aaaggaaatc tgtttcattg gttatccttg
    14401 ttgtctgaat gagggcaatt tcaacaggcg tttaatgatg gcaaaaccgg ctaaaatttc
    14461 ccacagcata atggtataaa tacctgctcg ttcaattaac gggctaaaca gggttggtag
    14521 gaaaatcgtg attaaaatac agattgcccc gataacgccc gcaccaatgc taaagcgttt
    14581 aaaaaattgc attgaagggt ggtagagatc gaagcctgcg atgatcagcg ttaaattgcc
    14641 gattaaaaac gtaatgcttg cccctaaatt gtaaatgccg aattcgccac gactgcttgg
    14701 ctgaataccg gcaactagtg agcagccaat cgcaaagcat agcgaaagca ataagccgat
    14761 gacccgcaat ttgttgtgag caatgtgggt gatgccgatt gcataaataa agaaaaaaac
    14821 gactgcttgt agcacaaaca ttgaacgcat taccaccgaa agtggtgata attcgctgat
    14881 tttttcatct atcattgtgc cgactggaat catcaattca agggtgctat ggtggaagta
    14941 agtggtgagc gatctttcgc taaataaaat cgtgatgtgt tccgccacca aataaaatag
    15001 cccgctaaaa cttgaaacca taccaataat aaaaatcaaa aaggcacagc ttttacgaaa
    15061 acaagggtgc attagaaaag ggtagaattt caccccaaat gaaccgctgg taaagtagtt
    15121 ttcgatggct ttggaaagcg tggttaattg ttgaattaaa tgtttaatcc actctgtttt
    15181 catataaacc tatcattttt aacgggttga gtgagttatc gtattcgtac gtacatcaaa
    15241 cacgttcata ttgccgttga gggcggcttg cacgcctaaa tcggcatctt caaacaccaa
    15301 acagcggtgt ggggcaatgc caagtttttc cgcacatttt aaaaaggttt caggggctgg
    15361 cttatggttg cttacatcat cagaatctac aataaccgaa acatattgac gtaagttaaa
    15421 tttttggtct aataattcca ccattgcacg gtgtgagcct gtgccgattg ccattggttt
    15481 tttgccaacg ttagctttaa taatctcaaa ggcaggcaac agagtggcat tggctaacac
    15541 catttcaatc ccaaattccc gttttagttg taccacttgc tcaaaataat gctcggggat
    15601 attggagcgt tgcattattt ctcgggcaat cacaaaagtg gttgagccac tcagttcgta
    15661 cattggcttt ggattgattg ggtagcctaa cacttcgccc accttttccc acgccttggc
    15721 gtggcttggc atcgtgtcaa ttaatgtgcc gtccatatca aaaatcaagg cgtcatattg
    15781 gtcgataatg gtttgatcta acattgcttt ccttaaattt ttttctaaat tctggattta
    15841 attccgccaa attcggcaat cattttttcg gtgaaaagtt tcagctcttc ggtcattaat
    15901 aagaaatcgg catcaaagcg ttgagccaca tcttctttga gaatatcgtc atttttctct
    15961 cgcacttcat cggcaaactt caggcgtgaa agcgtggcgt cttcatttaa cacgcaattg
    16021 aaatgccctt cccattcaat cgcaagtttt gtgataaatt tgcccgcttg caagtgccgg
    16081 gcgatttctt ccgtctctaa atcttggcgt ttacagcgaa taatgctgtc ggtatcgaaa
    16141 gatttcagtt ctgcctcttc caacaaattc aaccaacttg gcgtatgccc tttggcgatc
    16201 caattcgtca ttacctctgc cggcggtagg gtaaaggaaa tcggcacaac cggcaacgag
    16261 ccaagtgttt ttcgcagtaa cgccagcgta tcttccgccc gtttgctcga ggctgcatcc
    16321 acataaacca actgattatc taaatccaac caaagagcgg tgaattgatg tttgctgaat
    16381 gctcgaggca ataacgcaga aacgatctga tctttcacgg cttgtttttc ggtttttttc
    16441 agcttacgag cctctttttc ttccagttct gcaatgcgtt gctcggtttc ggctttcacc
    16501 acattagcag gtaataattt atcttctttg tgtgaaatca gcaaaaattg gttcgcttgt
    16561 gagaaatgca gtaaatccga gccggtaagt ggcgtagccc agccgaattt gctcatatcg
    16621 tgctggctgc aaggggtaaa gcgggtttct tgctgtaact cgttaaggct attgctttcc
    16681 aacgccagct cgccggttaa tttatagatc atcacatttt tgaaccaaac catttttgca
    16741 tatcctttat cgggtgtaga atagtgttat tttaacagac cccaacccaa cacagtagag
    16801 aatttgcgaa tgaacgagaa aaaacttgcc tttatcggca ccggaaatat ggcgtatgcg
    16861 attattcaag gcttattaaa aagcggttat tctgccgaac aaatcattgc gtgcaataaa
    16921 agtaatttgg ctcgccgaga ggagttacaa gcggtcggaa ttgcgacaaa ttttgcaaat
    16981 cgtgaggcgg tagagcaggc tgacgtgatc gtgctggcgg taaaaccaca gatgatggca
    17041 caagtgtgtg ccgagtttgc cgatgttgat ttttccaaca aatgggtgat ttcagtagca
    17101 gcaggtattt ctgtgcaacg cttggaaacg ctgttgccga gtgcgaaaaa tatcattcgc
    17161 acgatgccaa acaccccgtc tttaatcagc gaaggaatga ccggattatt tgcaaaaaaa
    17221 tcggtaaatc cgaccgcttg ccaatttgcc gaacagttga tgtcagccgt tggtaaatgt
    17281 tactgggtgg aaaccgagga gcaaatcaac cacattattg cgataaccgg ctcaagcccg
    17341 gcgtatttct tccgttttat ggaggcaatg cagcaaagtg cggtgcaaat gggcttttcc
    17401 gagcaggacg caagaacgct tgtacaatct gtcgcattgg gggcgacgaa attggtggaa
    17461 gcgaacccga ctacgccact ttcgaccttg cgggaaaatg tgacctcaaa gggaggcaca
    17521 acggcgaaag cccttgaggt gtttgagcag cacgatcttg ctaaaaccgt tgatgatgca
    17581 atgtgggcgg caattcatcg agccagagaa atggagcaat cgttatgatg aaagtttccc
    17641 cgttccaatg ggcggcattt aattactttg gctttttttg tgcctatggc gtgttgttgc
    17701 cgtttttgcc agtgtggctt aaacatcacg gctattcgac tgaaatgatc ggcttatttg
    17761 ccgcagttgg ctatttgttc cgctttgccg gcagtatgct tgcctcacaa cgggtaaaaa
    17821 ccgtgagcca actcattccc accgcccgca gtttaacttg gctgaatatt gcggctgcgg
    17881 tggcgttgct gttttcaggg gatttcattt ggctactttt tccggtgtta atgttgtttc
    17941 aaatgttcaa ttcgggggca atgccgatag ccgatagcat tgcctcaatt tggcaacatc
    18001 agatcgggct ggattacggc agagcaaggt tattcggctc aatggcgttt gtggtaggct
    18061 cactttctgc cggctatctt atcggcttgc ttggtgaaaa ttcggtggtg tggattatca
    18121 tcggtttctt ggttttttta ggaatagggc agcttgctac gcccaaagtc gggtttgaag
    18181 aacaaggtaa taaagccaat acttcaagcg taacttatcg ccaattattg aaagagccgg
    18241 aaaccttaaa aatgttgatc gcggtgtcgt taatttcagc ctcgcacgcc gcttattaca
    18301 cttacagcac cattcattgg tcaaatgcca gtatttcaac cgcaatgact agcctgtttt
    18361 ggggcttggc ggtggtggcg gaaattttgt tctttttggt ctctaaacgc ttgtttaaat
    18421 cgtggaaaat cagccattta atgattttag ctgccgtttt tgcagtgatt cgttgggcaa
    18481 taatgggtag tactacagag atcgtgctga ttattgtaat gcaaatgcta cattctatta
    18541 ccttcggggc ggctcatatt gcgatgattc gctatatttc gatgcaatcg gcagagcgtg
    18601 tgacgaaatt acaaggtctc tattttggct tggcaagctg tgcttttatg gcagtattta
    18661 cctttattgc cggaatggtc tatcaacatt ccccaagcct gacattttgg ttgatggcaa
    18721 tttttaccct acctgccatt tttattgtgc cgaaaaggtt tgaagtacag attaattgat
    18781 agcaaaaatg ccgacaagtt gtcggcattt ttatcgaaat tcattgaatt tgctgaaatt
    18841 agattgtttt cacgtttacg gctgaagctc cacgctctga atcttggatt tcaaattgta
    18901 ctttagcacc gtcttctaat gtgcggtagc cgccgtttgc ttcgatagca gagaagtgta
    18961 cgaatacatc tttgccacct tgatcagggg tgataaaaac caaaaccttt ggtggcgttg
    19021 aaccatttta ctgtgccggt aattgctgac ataaaatttt ccttatataa atacaaaaag
    19081 tgataaaaga tagctaaacg ggatattaca gacgtatcta tcaatcactt gccttgagga
    19141 aaataactaa caagattcaa ctttaattaa cgcacgaaca gcttgagcca ttacaccact
    19201 tattcatttt ttaagcaaat catttttata cttttatgaa cattcaaatg aaaaaagagt
    19261 ctatctcttt tgtaaacgta atgtggaatt tatatggacg aagccttata aatcaaatat
    19321 agtatccagt tagattttca cttatctaat tattagattt ttattaaatg ggacttatta
    19381 tgtcagagca aattgagcaa aaagtagaac aaactcagaa taaaaccaat ccaagtacca
    19441 aggaagtgat tgcttattta gcggagaaat ttccgctttg tttcacgctt gaaggtgaag
    19501 taaaaccatt gaaagttggg ttgtttcagg aattagctga agcattaaaa gatgatgaga
    19561 aaatcagtaa aacgttattg cgccaagtat tgcggaatta cactaattct tggcgttact
    19621 tggctgccag ccaagctaat gttgctcgtg tcggcttaca gggtgaagaa gttggcgtgg
    19681 taacagaaga cgaagctact cacgccgcag aagctttggc tgctgcgaaa gtggttgcag
    19741 aacgtaaagc acaagagcgt aaagcacaac gtaaagagta cttcaaacag aaagcacgtg
    19801 aagaaaatgc gaaaaagcgt gcagaattta aggcgaaaaa agcaatagat aatttaccta
    19861 aagcttcaga agaaaaatta gctgcattag cagataaatt cggtcgtaaa tagttttcaa
    19921 gtcgctttgt gcttacaagc ggtcggattt tagtaaaatt ttgcaaaaaa atgaccgctt
    19981 gattatttgg aaaagaagat gaaattaaca agattaaata aaatttttgc cctagcccta
    20041 ggtttaatga taggaaacgc ttttgccgtt cagccggaca ttaaagaaag tgcgattgtg
    20101 atgccaaaac ctaatgagca gcatagctta tcaaccaagc gtgtaacagc ccgattaact
    20161 caatcgcatt atcataaatt tcaactgaat gatgagtttg cagggaaggt ttttaaccgc
    20221 tatgtcgatt ggcttgatcc ttctcataat acttttcttc aagccgatat tgatgaaatg
    20281 cgtagtaaat atgctacaac attagatgat gatttatatg ctggtaaatt agatgcagca
    20341 tttgcaatgt atgagttaat gtctaaacgt cgttatgagc gttatagcta tgccttatct
    20401 ttactagata aagagcctga cttaaaaggg aatgatgaaa ttgagattga ccgtgagaag
    20461 gccgcatttc caaaaagtgt agaagaggca aatacacttt gggagcagcg agttaaaaac
    20521 gatattatca atttatatct taaagataaa aaatggcttg aaattaagaa aacactcact
    20581 aaacgttata actttgcgat taagcgttta actcaagtta aggctgatga tattttacaa
    20641 acttacttaa attcattctc tcgtgagatc gatccccaca ctagctattt gtctcctcgt
    20701 gcggcaaaag cgttccaaga gagcatgaat ctgtcgctag aaggaattgg tgcaacgcta
    20761 acccaagaag atgatgtaac cagcattaga gcattagtgc caggagcccc tgccgctaaa
    20821 agtaagaagc tggtagtcgg cgataaaatt gtcggtgtag gtcaaggtga gagaggtgag
    20881 attgaagatg ttatcggttg gcgtttggat aatgtagtcg ataaaatcaa aggtaaaaaa
    20941 ggcactaaag ttcgtttaga aattgagcct gaaaaaggtg gaaaaactaa aattattacc
    21001 cttgtgcgtg ataaagttcg tattgaggat agtgctgcta aattaacggt agaaaaagta
    21061 gatggaaaaa atgtaggggt aattaagatc cctacttttt atatcggttt gacttcagat
    21121 gtacgtaaat tacttactca aatagagaaa aaaaatgttg aaggattgat cattgattta
    21181 cgtgaaaacg gtggtggttc gttaactgaa gtgattgaat taactggttt atttattaaa
    21241 gatgggcctg tagtgcaagt acaagacgca ttcaatcgta tcaaagtaca cgaagatcca
    21301 gaatcaaaat cagtttatga tggaaaagtg atggtgatga ttaatcgcca tagtgcttca
    21361 gcctctgaaa tcttcgctgc ggcaatgcaa gattacaacc gtgcaattat tgtagggcaa
    21421 caaacctttg gtaagggaac tgttcaacag agccgttcat tgaattttgt atatgatttg
    21481 gatcaagagc ctcttggttt tatccaatat acgattcaaa aattctaccg tatcaatggt
    21541 ggaagtaccc agctaaaagg ggttgaggcg gatattaaat tcccggaaat cattaatgct
    21601 gcaaaaacag gtgaaagttt tgaagataat gcgcttcctt gggataaaat tccagcagct
    21661 aattataaag aggctgggaa tgctcgtaac gcggttgctg cattaacagc gaaacatcaa
    21721 gagcgtattg ctaaaaaccc agagtttata gtattaaacg agaatattgc gattcgaaaa
    21781 gaacgtgatg agcgtaagtt tttatcgtta aatttagaag aacgcctgaa agaaaataaa
    21841 gcagatgaaa gcaagcgctt aaaagatatt aatgctcgtt ttgcccgtga aggcaaaaaa
    21901 gcattaaaag atattgatga tttaccaaaa gactatgaag caccggactt tttcttaaaa
    21961 gaagctgaac agatgatgct tgattggtca gaatttgata aaaaatcata aatgtgttta
    22021 taatgcgcac ataacgtacc gcttagctct gctaagcggt ttattttttt ataaatttgc
    22081 aaatattaag aggaagagtg ggtgaaaacc tttaaattat tacctattat ggtggcgttt
    22141 tctggcatgg ctttggctga aacagccaca gttgctcagg tggcttctga gcctgttgta
    22201 caagataata ttttaccaca attaagtttt gcagctcagc aggctcgtgc tgcagaattg
    22261 gtaaaacagg ccgattatgc gttagaacag gaacaaattc gtcaagagca agcaagaaaa
    22321 tttgcagaag cggaagcaag aattaagcaa gaaataggta ataatgcctt attgctgagc
    22381 caagcaacaa ctaaggttta tttagacagt gaatttgcga caatgtggaa tgataaagcc
    22441 gcagaaaaag catttttgaa agaatatgca ttatttgcag taagtggggt atcctctaaa
    22501 tcagctaagg cattgcagca aatcttaaat caaccggaag gtttaggtag agatatttta
    22561 ttaactgatg gcttcttaga ttatttgtat tacaacaaaa atgtgtttaa aaatgcaaat
    22621 aattggttat ataacttagg cagttatgta ccaaaatcgc cctcttcaga gcaaatcttc
    22681 caatggacta atgccgtgag ggaaggcgat tctgggcagt ttgtaacaaa ccttgttcca
    22741 agaaaccatt tatatcgtga tacggcacag agagtattgg caatggcagg aggctcttca
    22801 gcacctaaag ccaaaaaagg taaagctgtt acggaggtag ctggtggcgg taatggggct
    22861 tctttctata aattagcgct caatgctcaa cgtttacgta ttattccaag ttttaacaat
    22921 ggaatttttg tgaatatccc aagctatcaa ttatattatt tccgtgatgg taatttagct
    22981 cttcaatcta aagtgattgt tgggcgagaa gatcgccgta caccggtaat ggatagtaag
    23041 ctgagcaatg tggtagtgaa tccaccttgg aatgtgccga ctagtattat gactaaagat
    23101 attattccta aactagcgcg tgacccaggt tatgcagatc gtgctagttt cgaaattctt
    23161 gatagtagtg gcaataaaat taatccgcgt agcgtaaatt ggtcacaata tttaaatgct
    23221 aaaagttcac cataccgtat tcgccagaaa gcaggtgatg atagcgcttt aggacgttat
    23281 aaatttaata tgccaagttc ggatgcaatt tatttgcatg atacgccaaa tagaggatta
    23341 tttggtaaaa cggggcgtgc gttaagttct ggttgtgtcc gtgtagaaaa atctgatgaa
    23401 ttggcaacca ttttattaaa agaagcgggt tggggatctg ataaaaagaa tcaagtctta
    23461 ggcagtaaga agacgacctc ggctcagatt cgttcagata acccggttta tctctattat
    23521 gtgacggctt gggttgaagg tggaaaagtg caaacccttc cggacattta taactttgat
    23581 gccaaaatcc cacaacattc tgtgaattgg ggaaaattaa agagcattat ttaatcgtgc
    23641 tatttctgat aaattccaag aagatatcat cttcttggaa ctttttgaaa atatagctta
    23701 cataaattta cgattgtaaa tttttacttt aagtatggtg tatagaaatt cgctcatttg
    23761 tgagtgattt tctataaatc attagctaac ctatgaggaa aaaatgaaac aaattaatgt
    23821 tcaacgccgt aagtggctat cactcggcgg acttgtttta ggggctagcc ttctgccggg
    23881 tcaagtaatg gcagcgcttt ctactcctgc cccagctgcg ttacgttttc gtaatgtgaa
    23941 tacaggcgat acttacactg ctaaatttgg tgcaggtgga ttaaataaaa ccgatttaag
    24001 ccagttaaac tatttaatgc gggatcgtca tatcaatcaa gttaaagcaa ttgatccaaa
    24061 attatttgtc aaattgaatc aattacaacg ccgtttaggt ttccataatg cggaaatcct
    24121 agtattaagt ggctatcgct ccgcacaaac aaatgcaaga cttcgtcgta gaagccgtgg
    24181 tgttgccagc catagttacc atattcttgg gcaagcagta gatttccaag tatcaggtgt
    24241 gccgttatat aaagtaaaac aagcggctga gagcttgaat aatggtggcg taggttatta
    24301 tcctcgtagt aactttgttc acgtagatac tggcccagtt cgcacttgga gaggctaaca
    24361 agcggtctta ttttatgttt aatttgcaaa ttattcctgt tagtccattt caacaaaatt
    24421 gcacggtgat ttgggacgaa aataagaatg cggctattat tgatggcggc gataatgccg
    24481 aaggcattat tcaatttgtt gaatctcagc agcttaaggt tcagaaatta ttgattacgc
    24541 acggtcattt agatcatatt atggcggtgg aaaagttgag agagcatttt caagtagagg
    24601 ttttcggctc gcatcttgca gataaacctt tatttgaacg tttgccggaa atgtgccaac
    24661 aatttggatt accaccagta aatggctttt tgccggatca ttggttggag gaaggtgatc
    24721 gggtcgaagt cggcgagctg agctataccg ttcgacattt gccgggtcat gctccgggtc
    24781 atatcggttt ttttgatttt gacaatcgta ttgcttttag tggcgatgtt ttattccaaa
    24841 atagcatagg tcgaacagat ctctatttag gcaaccatag ccagttattg gaaacgatca
    24901 gggaaaaaat gtttgattta gatgatgatt tcatcatcat accgggacat ggtccacaca
    24961 caactatcgg gcgggaaaaa cagaccaacc cgtttttgaa ataaataaag aagcagagca
    25021 gttggctctg cttttttgct attttacgcc tcgtaaatag aggcggtaat tatggctttc
    25081 ttccgtcggt ttaatgagtg caatggtctg tttttgctta ggttgcaacc atagatgttg
    25141 ccagccgata ctatctgata taccgttatg ggattgtgtt accccatctt tattatacca
    25201 aaataattta taagtaagat taagcggaaa t
//
LOCUS       DANJWC010000017        24070 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.017, whole genome shotgun sequence.
ACCESSION   DANJWC010000017 DANJWC010000000
VERSION     DANJWC010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 24070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24070
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.017"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..244
                     /locus_tag="PWX02_000502"
     CDS             <1..244
                     /locus_tag="PWX02_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HDL1728543.1"
                     /translation="NPSLPIKMVEHCVKELLEQLTATLEEGERIEVRGFGSFSLHYRQ
                     PRLGRNPKTGESVLLGAKYVPHFKAGKDLKERVDLL"
     gene            281..568
                     /locus_tag="PWX02_000503"
     CDS             281..568
                     /locus_tag="PWX02_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HDL1728544.1"
                     /translation="MIKYILGILIVIAVIIVAVTIGANNDQLITFNYIFAQSELRLST
                     LVAILFGLGLILGWLVTGIFYLKIKFQNVALNRRVKRQAQQITELTTPKAE"
     gene            572..1768
                     /gene="lapB"
                     /locus_tag="PWX02_000504"
     CDS             572..1768
                     /gene="lapB"
                     /locus_tag="PWX02_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HDL1728545.1"
                     /translation="MLELLFLLLPIAALYGWYMGQRSAKKDQETASNKFSREYVTGLN
                     FLLSNQQEKAVDLFLSMLQKQESENHIASESQFEAELTLGNLFRSRGEVDRALRIHQT
                     LVNSPHYSFDQKLLAKQQLAKDFMVAGFYDRAENYYISLVDEPEFAKYSLSQLAVIYQ
                     KTREWKKAINVTEKRLRIEPDADKIPLSHFYCEYAQGVKFDDKAAFIESLNKALEYYP
                     QSTRASIILGDFYLEEGEYLRALSYFEQILVQAPDYISEVLGRIKQSYMALEDLNGYE
                     LFLIRANRVKHNSSVDIALTEFIEEKDGINAAHSKLYQQLSTYPNLITFHRFIRYQAD
                     FAEEGNGKESLVLLHNMVGNQIKRSFQYRCLNCGYQSYRLMWQCPSCNQWEKIKPVQS
                     IEGIIQ"
     gene            1792..2484
                     /gene="pyrF"
                     /locus_tag="PWX02_000505"
     CDS             1792..2484
                     /gene="pyrF"
                     /locus_tag="PWX02_000505"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDL1728546.1"
                     /translation="MNNKVIVALDYETEREALEFVDLVDPSLCRLKVGKEMFTTLGTN
                     FVKQLHERKFDVFLDLKYHDIPNTVARAVRSAADLGVWMVDLHASGGLAMMEEAKKIL
                     EPYGKEAPLLIAVTVLTSMEDLDLLQIGINASPMEQVIRLAHLAQRAGLDGVVCSPQE
                     VEVLRTHCGQDFKLVTPGIRPKGTDFGDQRRVMTPKQAVETGSDFLVIGRPITQAQDP
                     LAVLKSINQSIS"
     gene            2567..2878
                     /gene="yciH"
                     /locus_tag="PWX02_000506"
     CDS             2567..2878
                     /gene="yciH"
                     /locus_tag="PWX02_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HDL1728547.1"
                     /translation="MTLVYSTETGRIKSEVIKEERPKGDGIVRIQRQTSGRKGKGVCL
                     ITGLDLEDKALNSLASELKRKLGCGGSFKDGVIEIQGDNRGFIKQILEQKGFKVKLAG
                     G"
     gene            2981..5143
                     /locus_tag="PWX02_000507"
     CDS             2981..5143
                     /locus_tag="PWX02_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HDL1728548.1"
                     /translation="MWKKTLIAVFLASQYAFAVGTGKEPSKSYTETELKQLKNDWLAI
                     YQKELQYAENLRLKQRENFQQINYLADAAISQKNLSLSTEQIIQRLLLTLAGYPLEMK
                     AEWKLLNAKITLKQATESEVKAFISKYPDSLYQRQLQNLMFELLYREQKFPELMEYAQ
                     NVKPSSLADQCRLFSARYELAASKAQINPEVSQVTEQKAENAELLALVQEFDRFWLAT
                     PKLTSDCANLESYWRDQGFKTDEKVRLKAVELIKQNANAELANLAVNVNNENLKIWLS
                     SVEKVANNPADLQNFIENQPLDSPFYAENKAIIQQLFAKYVRTLSENMENPSFQQYQA
                     WAEKYQFSNEEMNSWKNTFITRLFDNPEPTFQLWRDEQIKLLNTDNLTERRLRMAIWQ
                     QTALNEWLDLLSTEAKGKTEWRYWAAKEEKNEAKRKADFENLAKERGFYAMLAAQQLG
                     IIYQIPMLEVPELTQTQLELYENQLARIQELRELGQFEQARRVWIDWLKNIKAEAGEN
                     TPTKIQLALIKYAKDKNWYDLAVEGTIQIKAFDYLDLRLPNAYSDWFDLHLQDKKISK
                     TFAQAIARQESAWNAQAQSHANARGLMQLLPTTAQKTAKDNGLTFKDGNDLFQPFNNI
                     MLGTTHLMELNEKYPNNRILIASAYNAGAGRVEQWLKRSNGQLAMDEFIASIPFYETR
                     GYVQNVLAYDYYYQMLYSDINDKNGLKMFYQEELTRKY"
     gene            5196..5272
                     /locus_tag="PWX02_000508"
     tRNA            5196..5272
                     /locus_tag="PWX02_000508"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5230..5232,aa:Met,seq:cat)
     gene            5499..5960
                     /gene="rimP"
                     /locus_tag="PWX02_000509"
     CDS             5499..5960
                     /gene="rimP"
                     /locus_tag="PWX02_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDL1728549.1"
                     /translation="MATLEQKLEDLVLDSIEALGFELVGIECQRAGRFLTVRLYIDKE
                     GGVTIDDCSDVSRQVSAIFDVEDPIADKYNLEVSSPGLDRPLFTVAHYERFIGQDVVI
                     HLRIPMFDRRKWQGKIKQVEGDFITLTVENEDCQFAFGNIQKANLVPVFNF"
     gene            5981..7477
                     /gene="nusA"
                     /locus_tag="PWX02_000510"
     CDS             5981..7477
                     /gene="nusA"
                     /locus_tag="PWX02_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDL1728550.1"
                     /translation="MSKEILLAAEAVSNEKQLPKEAIFEALETALTIATKKSRDLDID
                     VRVVVDRKTGDFQTFRRWAVVENVNNMTREISLEAAQFEDPNVQLGDFIEDEVESIEF
                     GRVAIQTFGQVVKTKIREAEKTKIIDEFRSDLGKIILATVKKVTRDNIFLELQGKSED
                     IKGEAVIVREDMIPRENFRPGDRVRGVLYEIKPESKGPQLFVTRAKPVMLEELFKLEV
                     PEIGEGVIEIKGSARDVGSRAKIAVKTHDKRIDPVGACVGMRGARVQAISNELGGERV
                     DIVLWDENPAQFVINAMAPADVSSIVINDETHSMDIAVEAKNLAQAIGRNGQNVRLAT
                     QLTGWALNVMTVEELTKKSEEEDGKVINLLTSALEIDEEFAQVLIEEGFTSLEAIAYV
                     PVDTLTAIDGLEDEDLVEELQSRAKNAITKKALEEEKLLKSAQIEEKLLTLEGMDRHI
                     AFKLAEKQITTLEELAEQGVDDLADIEELSEEKAGELIMAARQICWFN"
     gene            7462..10029
                     /gene="infB"
                     /locus_tag="PWX02_000511"
     CDS             7462..10029
                     /gene="infB"
                     /locus_tag="PWX02_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDL1728551.1"
                     /translation="MLVQLSKEDNKMTEEKKTLSLGGARKASKVNTTTTSGKVKAVEV
                     KEKKPKIDAKALQEKAAKEAAAAKARAEKEAAEKLAKEKAEAEAKEKAAKKAAESTAS
                     KPISVPVMPSSKAKEEKPKAQQVQPKQEKAVDPEKEAKRKEEAELRRKQEELAQQKAE
                     MEAKRAAENARRLAEIAREEAEDNGNEDFDDERFTSSYAREAERDNDRRSEGNRGRNG
                     KGNVAKAKKGGREEDKNERSADRRNHKEVKGKGKQGKKGAALQQAFTKPAQVVKADVV
                     IGETITVAELANKMAVKATEIIKMMMKMGEMVTINQVIDQETAQLVAEELGHKVILRK
                     ENELEEAVLEDRDTHAEKVTRAPVVTIMGHVDHGKTSLLDYIRKAKVAAGEAGGITQH
                     IGAYHVETDDGKMITFLDTPGHAAFTSMRARGAKATDIVVLVVAADDGVMPQTIEAIQ
                     HAKAAGAPLVVAVNKIDKPEANPDRVEQELLQHEVISEKFGGDVQFVPVSAKKGMGID
                     DLLEAIILQSEVLELTAVKDGMASGVVIESYLDKGRGPVATILVQSGTLNKGDIVLCG
                     FEYGRVRAMRDENGKDVESAGPSIPVEVLGLSGVPAAGDEATVVRDEKKAREVALYRQ
                     GKFREVKLARQQKAKLENMFSNMAAGDVAELNIIVKADVQGSVEAISQSLMELSTDEV
                     KVKIVGSGVGGITETDATLAAASNAIMVGFNVRADASARRVIENENIDLRYYSIIYEL
                     LNDVKAAMSGMLQPEFKQEIIGLAEVRDVFRHPKFGAIAGCMVTEGIVKRNNPIRVLR
                     DNVVIFEGELESLRRFKDDVSEVRNGMECGIGVKNYNDVKVGDQIEVFEVVEVKRTI"
     gene            10114..10494
                     /gene="rbfA"
                     /locus_tag="PWX02_000512"
     CDS             10114..10494
                     /gene="rbfA"
                     /locus_tag="PWX02_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDL1728552.1"
                     /translation="MSREFKRSDRVAQELQKEVAVILQREVKDPRIGMVTVSDVEVSR
                     DLAYAKIFVTFLFDNDAEAIKQGMQGLEKASPYIRTLLGKAMRLRIVPELRFIYDESL
                     VEGMRMSNLVSSVIKNDEEKHKED"
     gene            10497..11420
                     /gene="truB"
                     /locus_tag="PWX02_000513"
     CDS             10497..11420
                     /gene="truB"
                     /locus_tag="PWX02_000513"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDL1728553.1"
                     /translation="MSRPRKRGRDIHGVFLLDKPQGMSSNDILQKVKRIFQANKAGHT
                     GALDPLATGMLPICLGEATKFSQFLLDSDKRYLVTAKLGERTDTSDAEGQVVETKAVA
                     VTKSQILAALEKFRGDILQVPTMFSALKHNGKPLYEYARAGITVEREARPITIFELNF
                     IEYKAPFLTLEVHCSKGTYIRTLVDDLGEELGCGAHVTMLRRTAVADYPIEKMMSYAD
                     LQNLAENQPLAELDQHLLPMDTAVSALAKINLSSEQTKAVGFGQRIRFDNLNQLYGLV
                     RLYSEDEQFLGVAEITNDTVIRPNRMINLTE"
     gene            11423..12517
                     /locus_tag="PWX02_000514"
     CDS             11423..12517
                     /locus_tag="PWX02_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HDL1728554.1"
                     /translation="MKSTIFTLPLAIITLALAGCSSDNQESYAGLNLDAKYSKSRSVS
                     HFNDYVHFLKQKAAGAGVSAKTLNAQKFIHYNARAVQLDQQQAARRRDPNVPPPPPNP
                     SGVTHYLNKVLTQAKVDQASERWYEYNSQLTHASQKYGVQKEYILALWGMESSFGRNQ
                     GDFDVLSVLATLAFDGRREKLFTQEFVNAMKMLDDGTIKRYELLGSWAGAMGQTQFMP
                     TSYLTYAADGNNDGKKDIWENEADAFASIANYLSTVGWDNQLPWGVEVKLASPMDLSF
                     AGLETNKAKTLAEWQALGIYLAYPNGQESAKLAQLHGKEVWLIRPDKEAGRAFLVSNN
                     FRTLMDWNKSNNFGISIGKFADRILASVGQ"
     gene            complement(12555..13079)
                     /gene="tadA"
                     /locus_tag="PWX02_000515"
     CDS             complement(12555..13079)
                     /gene="tadA"
                     /locus_tag="PWX02_000515"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDL1728555.1"
                     /translation="MFIKPNQTSCPLQISEQDIAFMQYALDLADLAEAKGEIPVGAVL
                     VDKNQQIIGRGWNQTIQLCDPSAHAEMQAIRQAGQTLGNYRLLDCTLYVTLEPCPMCA
                     GAILHSRIKRLVFGASDYKTGAVGSRYHLFEDYKMNHFLEVQGNVLGRECSQKISDFF
                     KQRRLQKKGDALIK"
     gene            complement(13161..14966)
                     /locus_tag="PWX02_000516"
     CDS             complement(13161..14966)
                     /locus_tag="PWX02_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL1728556.1"
                     /translation="MTTESLTFADLGLPQPILDAVNEMGFVNPSPIQQECIPHLLAGR
                     DVLGMAQTGSGKTAAFSLPLLAQIDPTQRHPQMLVMAPTRELAIQVADACEQFAKNMK
                     GIRVVTVYGGQRYDIQLRALKQGSQVVVGTPGRILDHIRRGTLDLSELKFIVLDEADE
                     MLRMGFIDDVETVMAELPETHQTALFSATMPEPIRRITRRFMKDPQEVKIKATERSAP
                     DIEQSYWLVNGFRKNDALLRFLEVEEFDAAIIFTRTKTGTIDITELCERNGYRTAALN
                     GDMTQQAREQTLDKLRSGRLDILVATDVAARGIDIERISLVINYDIPLEEESYVHRIG
                     RTGRAGRSGRALLFVEPRERRLLRNIENLIKKPINEVAIPNHEILMEKRREKFKARIS
                     KQLEHHDLEKYRELLEDLFTADQDHEELAAAMMMLLQEKQKLILPPDPVVRAARSERG
                     RDRNERNDRRNGREHRENNGVAMDLYRIELGREDGVEVRHIVGAIANEGDISSRYIGH
                     IKLYDKYSTIELPQGMPNHLVQHFAHKARVLSKPMQMTLLGSNSASNANPFEGRGRRG
                     NDRNDRGGRRDGGFNKDRKGGFKEKRFSEKRGRRD"
     gene            complement(15169..16092)
                     /gene="nlpI"
                     /locus_tag="PWX02_000517"
     CDS             complement(15169..16092)
                     /gene="nlpI"
                     /locus_tag="PWX02_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HDL1728557.1"
                     /translation="MVQLFKLFRIRFLLLNLISVLMLSGCTSRYADLVSIKNLALAEL
                     NPQLRFEQEAMVVRLSQVLEEAPLTPSEQADLYFERGVIYDSLGLWSLARYDFAQAIS
                     LNQRMAAAYNYMGLYLLLEGDYDSSIDAFNAVLELDREYSYTYLNRGLAFYYSGRYSE
                     AQRDLLHFYEEDKKDPYRALWLYFNELEFTPTEAKNNLQKRANNLSSDYWGTNIVNYF
                     LGKLNLAQVRAIMERDAKENSSQYAEILTETYFYLAKQQLKLDHKDEAISLFRLALAN
                     QVFNFVEYRFALFELSQIRGMTQTTPVVDKE"
     gene            complement(16173..18323)
                     /gene="pnp"
                     /locus_tag="PWX02_000518"
     CDS             complement(16173..18323)
                     /gene="pnp"
                     /locus_tag="PWX02_000518"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDL1728558.1"
                     /translation="MTPIVKQFKYGQHTVTLETGAIARQATAAVMASMDDTTVFVTVV
                     AKKDVKEGQDFFPLTVDYQERTYAAGRIPGGFFKREGRPSEGETLIARLIDRPVRPLF
                     PEGFFNEIQVIATVVSVNPQISPDLVAMIGASAALSLSGVPFNGPIGAARVGFINDQF
                     VLNPTTSEQKISRLDLVVAGTDKAVLMVESEADILTEEQMLAAVVFGHEQQQVVIENL
                     KEFVKEAGKPRWDWVAPEPNTDLINKVKALAETRLGDAYRIVEKQVRYEQIDAIKAEV
                     IAQLTAEDETVSEGTIIDIITALESQIVRSRIIAGEPRIDGRTVDTVRALDICTSVLP
                     RTHGSALFTRGETQALAVATLGTERDAQIIDELTGEKSDRFLFHYNFPPYSVGETGRI
                     GSPKRREIGHGRLAKRGVLAVMPTAEEFPYVVRVVSEITESNGSSSMASVCGASLALM
                     DAGVPIKAAVAGIAMGLVKEDEKFVVLSDILGDEDHLGDMDFKVAGTRTGVTALQMDI
                     KIEGITPEIMRIALNQAKGARMHILGVMEQAIPAPRADISDYAPRIHTMKIDPKKIKD
                     VIGKGGATIRALTEETNTSIDIDDDGTVKIAATDGNAAKAVMARIEEIVAEVEVNQIY
                     NGKVTRVVDFGAFVSILGGKEGLVHISQITNERVERVADYLTVGQEVQVKVVEIDRQG
                     RIRLTMKDINNTNEANAEETVAENVVETEQENNF"
     gene            complement(18585..19865)
                     /gene="phoR"
                     /locus_tag="PWX02_000519"
     CDS             complement(18585..19865)
                     /gene="phoR"
                     /locus_tag="PWX02_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HDL1728559.1"
                     /translation="MKVKLSLKHFLLELLLAAAVAGVFNLFFPAYFFWFSFCLLVLLI
                     WHHHTESSLLQLLRPNEQSGRSKVTTMEHLSQSAAFYHERLRQDKIKTLRLLSKLNKH
                     IQYLPDAIIICQENGEISWCNNVAQELFEFYWDKKSNKSIFNVIFYPEFKSYFRQQRH
                     HRPLVLLTHNKRYIELNLNRYDSNSRLIVARDVTSFVRLMHSRQTFLANMNHELRTPL
                     TILQGYLEMLENEPHNMALQQKALNAMKEQSQRMTNLLRQLSLLVKIEHSNDSEHNEV
                     QMSALISAIQKNTEILSQNQQILFDIEPNLTVWGDQEQLQSAVSNLIYNAIRHAGEGV
                     TIQICCQRDNQEPNTAIFSVQDDGIGIDAHHITHLTERFYRVDESRSNKTGGSGLGLA
                     IVKHALEQHHSTLEIESEAGKGSRFSFKLPLIKK"
     gene            complement(19868..20554)
                     /gene="phoB"
                     /locus_tag="PWX02_000520"
     CDS             complement(19868..20554)
                     /gene="phoB"
                     /locus_tag="PWX02_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006996031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HDL1728560.1"
                     /translation="MNNKILIVEDERAIREMLSLYLSKQYQVIEAEDYQSAINKLGEK
                     PELILLDWMMPGRSGIHLIKYLKKQPETEKIPIIMLTARSSEEDCITGLNAGADDYIT
                     KPFSPQVLLARIEAVWRRSYDPPSNLINIDGLILDQQAQRVLVAEQEVKLSGTEYKLL
                     HFLITHPEKVYRREQLLDFIWGNDIYVEDRTVDSYIRRLRKSLEPYGFERYIQTVRGS
                     GYRFSNHFQD"
     gene            complement(20638..21408)
                     /gene="pstB"
                     /locus_tag="PWX02_000521"
     CDS             complement(20638..21408)
                     /gene="pstB"
                     /locus_tag="PWX02_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HDL1728561.1"
                     /translation="MLPTDLETKLHINNLNFYYGDFHALKNINMRIAKNKVTAFIGPS
                     GCGKSTLLRTLNRMFELYPNQRATGEINLDGENLLTSDTDISIIRAKVGMVFQKPTPF
                     PMSIYDNVAFGIRLFEKLPKSELNDRVEWALTKAALWNEVKHKLNQNGDSLSGGQQQR
                     LCIARGIAVKPEVLLLDEPCSALDPISTMKIEELINELKHDYTVAIVTHNMQQAARCS
                     DYTAYMYLGELIEFGDTKQIFDKPKFRQTEDYIKGKMG"
     gene            complement(21418..22266)
                     /gene="pstA"
                     /locus_tag="PWX02_000522"
     CDS             complement(21418..22266)
                     /gene="pstA"
                     /locus_tag="PWX02_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005650601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDL1728562.1"
                     /translation="MKNYQNRRFYCRKIKNVLMLSLAYSAVLFGLFWLVWIIVTLLQK
                     GIPALSWELFTRPTPAPNETGGLRNAIVGSILIVSTATLIATPIGILAGTYLSEYGRY
                     TKLAKFTRFLNDVLLSAPSIIIGLFIYAIYVAQVKHYSGWAGSLALAVLVIPIVVRTT
                     DSMLNLVPNNLREAAAALGCPRWRVITMLSYRAARSGIITGVLLAVARISGETAPLLF
                     TALSNQFASLDMNAPMANLPVVIYQYAASPFADWNTLAWAGAMLITLFVLSLNIITRL
                     YFSKTK"
     gene            complement(22263..23222)
                     /gene="pstC"
                     /locus_tag="PWX02_000523"
     CDS             complement(22263..23222)
                     /gene="pstC"
                     /locus_tag="PWX02_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDL1728563.1"
                     /translation="MHKHKIDNKCLNHPWVEGLFHHTTRFFALLVFALLVVVLVSLLL
                     GSWEALSRFGVSFLWENEWNPVNDRYGALVPIIGTLISAGIALVIAVPVSFGIATFLT
                     ELAPQWLKRPVGTAVEMLAAIPSIIYGMWGLFVFVPLFQEYVQPYLIDTFGQLPFIGM
                     LFSGAPFGIGLFSAGLVLAIMIIPFIASIMRDVFSVVPPLLKESAYGLGSTTWEVIWK
                     VVLPYTKTGVIGAIMLGLGRALGETMAVTFVIGNAFMLPDSVFSPSTSIASAIANEFN
                     EAGGLQKSALMSLGLILFLITSIVLAASRLLIARMGKQEGKRE"
     gene            complement(23303..>24070)
                     /gene="pstS"
                     /locus_tag="PWX02_000524"
     CDS             complement(23303..>24070)
                     /gene="pstS"
                     /locus_tag="PWX02_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005756281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDL1728564.1"
                     /translation="LLQFPAIIGGTVPVVNIPELKSGELKLSGKVLADIFLGKITQWN
                     DPAIVALNQDIALPAKTILVVRRSDGSGTTFGWTNYLSKVSAEWKEKVGEGKSVKWPV
                     GQGGKGNEGVAAYVKQLKYSIGYVEYAYAKQNALAWVSMQNADGKFVQPSRETFAAAA
                     ANADWSQTAGMGVILTNEAGEKAWPITAASFILIHQHSDKPEQTKGVFKFFDWAFKHG
                     KDAAAELDYVPLPDNVVEKIQTQWKTEVKDGQGNSLY"
BASE COUNT         7249 a         4675 c         5344 g         6802 t
ORIGIN      
        1 aaatccaagt ttaccaatta aaatggtgga gcattgtgta aaagagcttt tagagcagtt
       61 aacggctact ttagaagaag gtgaaaggat agaagtaaga ggatttggta gtttttctct
      121 acattatcgg cagccgcgtt taggtcgcaa tcccaaaaca ggagaaagtg ttcttttagg
      181 cgcgaaatat gtaccacatt ttaaagctgg taaagatcta aaagagagag ttgatctgct
      241 ttaagtctta ttaattcaat aatcgtagta aggagattat atgattaagt atattttagg
      301 tattttaatt gtgattgcag ttattattgt cgctgttacg attggtgcca ataatgatca
      361 acttatcacc tttaactata tttttgctca gagtgaatta cgcttatcaa cgttagttgc
      421 gattttattt ggcttaggat tgattcttgg ttggttggta acgggtattt tttacttaaa
      481 aattaagttt caaaacgttg ctttaaatcg tcgtgtaaag cgccaagctc agcaaataac
      541 tgaattaacg actccaaagg ctgaataatc aatgcttgaa ttattgttcc ttttattgcc
      601 tattgcagca ctatatggct ggtatatggg acaacgtagt gcaaagaagg atcaagaaac
      661 cgctagtaat aagttctctc gtgagtatgt tacggggtta aattttcttt tatctaatca
      721 gcaggaaaag gcggtggacc ttttcctctc tatgttacaa aaacaagaat ccgaaaatca
      781 tattgcatct gaatcccaat ttgaagcgga gttaacccta ggtaatcttt tccgttcaag
      841 aggtgaggta gatcgtgcct tacgtattca ccaaacatta gtgaatagcc ctcattattc
      901 atttgatcaa aaattattag caaaacagca attagctaaa gactttatgg ttgcaggttt
      961 ctatgaccgt gcagaaaatt attacatttc attagttgat gaacctgagt ttgctaaata
     1021 ttctttatct cagcttgctg ttatttacca aaaaaccaga gaatggaaaa aagcgattaa
     1081 tgttacagaa aaacggcttc gaatagagcc ggatgcagat aaaataccgc tttcacattt
     1141 ttattgtgaa tatgcgcaag gggttaaatt tgatgataaa gcggcattta ttgaatcgtt
     1201 aaataaagcg ttagaatatt atcctcaatc cacacgcgct tctataatat taggtgattt
     1261 ctatttagaa gagggagaat atctaagagc attaagctat ttcgaacaga ttcttgtgca
     1321 agcccctgat tatattagcg aagtgcttgg acgaattaag caaagctata tggctcttga
     1381 ggatttaaat ggctatgagt tatttctcat tcgagcaaac cgagtaaaac ataatagtag
     1441 tgtagatatt gctttaactg aatttattga agaaaaagat ggtatcaatg ctgcacattc
     1501 taaactatac cagcagttga gtacttatcc gaatttaatt acttttcacc gctttattcg
     1561 ctatcaagca gattttgcgg aggaaggaaa tggtaaggaa agcttagttt tattacataa
     1621 tatggtggga aatcagataa aacgcagctt ccaatatcgc tgtttaaatt gtggttatca
     1681 aagttatcgc ttaatgtggc aatgtccttc ttgtaatcaa tgggaaaaaa ttaagccggt
     1741 acaaagtatc gaaggtatta ttcaataaag aaagaatctg aggaattgaa aatgaacaat
     1801 aaagtaattg ttgcgttaga ctatgaaact gaacgtgaag cattagaatt tgtggatttg
     1861 gtcgatccgt cgctttgtcg tttaaaagta ggcaaagaaa tgtttacaac cttaggcaca
     1921 aattttgtca aacaattaca cgaacgtaaa tttgatgtat tccttgattt aaaatatcat
     1981 gatattccaa atacggttgc tcgagcagta cgttctgctg ctgatcttgg tgtgtggatg
     2041 gttgacttgc atgcttctgg tggtttagcc atgatggaag aggcaaagaa aattttggag
     2101 ccttatggca aagaggcacc attattaatt gcagtgacgg ttctaaccag tatggaagat
     2161 ttggatttgc ttcaaatagg tattaatgct tcgccgatgg aacaagttat tcgtttagca
     2221 catttagcac aacgggctgg tttagatggc gtggtatgtt ctccacaaga agttgaggta
     2281 ttacgtaccc attgtggtca agattttaaa ttagtgaccc caggaatccg gccaaaagga
     2341 acagattttg gggatcaacg ccgagtgatg acacccaaac aagcagtaga aacaggctca
     2401 gattttttgg ttattggtcg tcctattacc caagctcaag atccgcttgc ggtattaaaa
     2461 tctattaacc aatctatcag ttaattcatt gcatttttaa tgaaggcgga aaaatttccg
     2521 ccttttttaa tcaaacaagc ggttattttt gaggaaaaat ttgtaaatga cattagtcta
     2581 ttcaacggaa acaggcagaa taaagtccga agtaataaaa gaagagcgcc ctaaagggga
     2641 tggtattgtg cgtattcaac gtcaaacaag cggtagaaag gggaaaggtg tttgccttat
     2701 caccggtctt gatttagagg ataaagcctt aaattctctt gcttcagagc ttaaacgtaa
     2761 attgggttgt ggtggctcat ttaaagatgg agtaattgaa atccagggcg ataatcgagg
     2821 attcattaag caaatcttag aacagaaagg tttcaaagtg aaactggcag gtggttgagt
     2881 tttaaacaat tatcagctgg cagtttttaa ataacttcat tttatttgaa acttcattcc
     2941 tacttttctg cctaaaactc acgattaagg agaaaaacta atgtggaaga aaacgttgat
     3001 tgctgtattt ttagcaagcc aatatgcttt tgcggtagga accgggaaag aaccatcaaa
     3061 aagttatact gaaacagaat taaaacaatt aaaaaatgat tggttagcga tttatcaaaa
     3121 agagctacag tatgcagaaa atttgcgttt aaaacagaga gaaaattttc agcaaattaa
     3181 ttatttggca gatgcagcga ttagtcagaa gaatttatca ctttcaacgg agcagataat
     3241 tcagcgttta ttactaacct tagcgggtta tccgttggag atgaaagctg aatggaagct
     3301 gctaaatgct aaaataacgt taaaacaagc tacagaaagt gaagttaaag catttatttc
     3361 aaaatatcct gattctttgt atcaaaggca gttacagaac ttgatgtttg agctgctcta
     3421 tcgtgaacag aaatttccgg agttaatgga atatgctcaa aatgtaaaac cgagtagtct
     3481 tgccgaccaa tgccgtttat tttctgctcg ttatgaactg gcagcaagta aagctcaaat
     3541 caatccggaa gtttctcaag taactgagca aaaagcagaa aatgcagaac tgcttgcttt
     3601 ggtgcaagag tttgatcgct tttggcttgc tacgccgaaa ttaacttctg attgtgcgaa
     3661 tttagaaagt tattggcggg atcaggggtt taaaaccgat gaaaaagtgc gtttgaaagc
     3721 ggtcgaatta attaaacaaa atgcaaatgc ggaattggcg aatttagcgg taaatgtaaa
     3781 caatgagaat ttgaaaatct ggctatcttc cgttgaaaaa gtagcgaata atccggcaga
     3841 tttgcaaaat tttatcgaaa atcaaccgct tgattctccg ttttatgctg agaataaagc
     3901 gattattcag caactctttg ctaaatatgt gcgaacttta tcggaaaata tggagaaccc
     3961 aagtttccag cagtaccaag cgtgggcgga gaagtatcaa ttttccaatg aggaaatgaa
     4021 tagctggaaa aataccttta tcactcggct ttttgataat ccagaaccaa cattccagct
     4081 ttggcgtgat gagcaaatta aactgctcaa tacagacaat ttaaccgaac gccgtttacg
     4141 aatggcaatt tggcagcaaa cagcgttaaa tgagtggttg gatttactct caactgaggc
     4201 gaaaggtaaa acagaatggc gttattgggc agcgaaagag gagaaaaatg aagcgaagcg
     4261 taaagctgat tttgaaaact tagctaaaga gcgaggcttt tatgctatgt tggctgctca
     4321 acaattaggc attatttacc aaatcccaat gctcgaggta cctgaattga cgcagactca
     4381 gcttgaattg tatgaaaacc aattagcgcg tattcaagag ttacgagagc tagggcagtt
     4441 tgagcaggca agaagagtat ggattgactg gctaaaaaac attaaagcgg aagcgggtga
     4501 aaatacacca acgaaaatcc aattagccct gataaaatac gctaaggata aaaattggta
     4561 tgatttggcg gtggaaggta cgattcaaat aaaagccttt gattatcttg atttgcgatt
     4621 accaaatgcc tattcggatt ggttcgattt acatctgcaa gataaaaaaa tcagcaaaac
     4681 gtttgctcag gcgattgctc gtcaagaaag tgcgtggaat gctcaggctc aatcgcacgc
     4741 taatgcgaga gggctaatgc aactgttacc gacaacagca caaaaaaccg ctaaagataa
     4801 cggtttaacc ttcaaagatg gcaatgatct ctttcaaccg tttaacaata ttatgttagg
     4861 cacaacccat ttaatggagt taaacgaaaa atatccgaat aatcggattc tgattgcctc
     4921 tgcttataat gcaggagcag ggcgtgttga gcagtggctg aaacgtagta acgggcagtt
     4981 agcaatggat gaatttattg ccagtatccc gttttatgaa accagaggct atgtacaaaa
     5041 tgtgctggcg tatgactact attatcaaat gctctatagc gatataaatg ataaaaatgg
     5101 attaaaaatg ttctatcaag aggaattaac aagaaaatat taaaaaatgc ttgtgttttt
     5161 gtgtagaacc gctataatgc accctatcag acagacgcgg ggtggagcag cttggtagct
     5221 cgtcgggctc ataacccgaa ggtcgttggt tcaaatccgg cccccgcaac cagttttaaa
     5281 ctcacagctt tggttaaagt tgtgagtttt gttttatatg ctgtagcaaa tttgcaaaaa
     5341 atttggcaaa aaagaccgct tgttgtggtt gctacagcaa aaaacaaacc gcttaaagcc
     5401 ccacggtttc ccgtctgggg cttttttgcg gaaattggta cagcattaac gtatcaaagc
     5461 tggtttatcc ggcttttttt atatttggag attatgactt ggcaacttta gaacaaaaat
     5521 tagaagattt agtgctcgat tctattgaag cattaggctt tgaattagtc ggcattgaat
     5581 gccaacgtgc aggccgtttt ttaacggtgc gtttatatat tgataaagaa ggtggcgtga
     5641 cgattgatga ttgtagcgat gtcagccgcc aagtgagtgc gatttttgat gtggaagatc
     5701 cgattgccga taaatataac ttagaggttt cttcaccagg gttagatcgt ccattattta
     5761 ccgttgccca ctatgagcgt tttatcgggc aagatgtagt gattcattta cgcattccga
     5821 tgttcgatcg ccgtaaatgg caaggtaaaa tcaaacaagt agaaggtgat ttcatcactt
     5881 taacggtaga aaatgaagat tgccagttcg catttggcaa tattcaaaaa gcaaatttag
     5941 ttccggtttt taatttttaa gataaaggta aactgacaaa atgagtaaag agattttatt
     6001 agctgcggaa gcagtttcaa acgaaaagca attaccaaaa gaggcaattt ttgaagcctt
     6061 agaaaccgct ttaaccattg caaccaaaaa gagccgtgat ttagatattg acgtgcgtgt
     6121 ggtggtagat cgtaaaaccg gtgatttcca aactttccgt cgttgggctg tggttgaaaa
     6181 tgtgaataat atgacccgtg aaatcagctt agaagcggca caatttgaag atccaaacgt
     6241 acaattaggc gattttattg aagatgaagt agagtctatc gagttcggtc gtgtggcgat
     6301 tcagactttt ggtcaggtag tcaaaaccaa aattcgtgaa gcggagaaga cgaaaatcat
     6361 tgatgaattc cgttcggatt taggcaaaat tattcttgcc actgtgaaaa aagtaacccg
     6421 tgataatatt ttccttgagt tacaaggtaa atcagaagat atcaaaggcg aagcggtcat
     6481 tgtgcgtgaa gatatgatcc cacgtgaaaa cttccgcccg ggtgaccgtg tgcgtggtgt
     6541 gttatacgaa atcaagcctg aatcaaaagg acctcagctt ttcgttaccc gtgctaaacc
     6601 ggtgatgtta gaagagctat tcaaattgga agtgcctgaa attggcgaag gcgtaattga
     6661 gatcaaaggt tcagcccgtg atgtcggctc tcgtgcaaaa attgccgtga aaacccacga
     6721 taaacgtatt gacccggtag gggcttgtgt gggtatgcgt ggtgctcgtg ttcaagctat
     6781 cagcaatgaa ttaggcggcg agcgtgtgga tatcgtactt tgggacgaaa atccggctca
     6841 atttgtgatt aatgcaatgg ctccggcaga tgtaagctct attgtgatta acgatgaaac
     6901 tcactcaatg gatattgcgg ttgaagcgaa aaatctggct caagctatcg gtcgtaacgg
     6961 tcaaaacgtg cgtttggcga cacaattaac tggttgggca ttaaatgtaa tgaccgttga
     7021 agagctaacg aagaagagcg aagaagaaga tggtaaagtc atcaatttat taacttctgc
     7081 tttagaaatt gatgaagaat ttgctcaagt tttaattgaa gagggcttta cgtctcttga
     7141 agcgattgct tatgtgccgg ttgatacttt aacggcgatt gacggtttag aagatgaaga
     7201 tttagtcgaa gaattacaat ctcgtgccaa aaatgcgatc acgaaaaaag cgttagaaga
     7261 agagaagtta ttaaaatcag ctcaaattga agaaaaatta ttaacgcttg aaggtatgga
     7321 tcgccatatc gcattcaaat tagctgaaaa acagattacg actcttgaag aacttgcaga
     7381 gcaaggtgtt gatgatttag ccgatattga agaactttca gaagagaaag ctggcgagct
     7441 aattatggca gcacgccaaa tttgttggtt caactaagca aagaggataa taaaatgaca
     7501 gaagagaaaa aaacactttc gcttggtggc gctcgcaaag cgagtaaagt gaatacaacc
     7561 acaaccagtg gtaaagtaaa agcggttgaa gtaaaagaga aaaaaccgaa aattgatgca
     7621 aaagcattgc aagaaaaagc agcaaaagaa gctgccgcag cgaaagctag agctgaaaaa
     7681 gaagcagctg aaaagttagc caaagaaaaa gccgaagctg aagctaaaga gaaagctgca
     7741 aaaaaagcag cagaatcgac cgctagtaaa ccgatttctg tgccggtaat gccaagcagc
     7801 aaagcaaaag aagaaaaacc gaaagcacaa caggtacagc caaaacaaga gaaagcggtt
     7861 gatcctgaaa aagaagctaa acgcaaagaa gaagctgagt tacgccgtaa gcaagaggaa
     7921 ttagctcagc aaaaagcgga aatggaagca aaacgtgctg cagaaaatgc ccgccgttta
     7981 gcagaaattg ctcgtgaaga agcggaagat aacggcaacg aagattttga tgatgagcgt
     8041 tttacttctt cttatgctag agaggctgag cgtgataatg accgccgtag tgagggaaat
     8101 cgtggtcgta acggcaaagg caatgtagct aaagcgaaaa aaggcggacg tgaagaggat
     8161 aaaaacgaac gcagtgcaga tcgtcgcaac cataaagaag taaaaggcaa aggtaaacaa
     8221 ggtaagaaag gtgcagcttt acaacaagcc tttaccaaac cagctcaagt tgttaaagct
     8281 gatgtggtga ttggtgaaac tatcactgtg gctgaattgg ctaataaaat ggcagtgaaa
     8341 gcgactgaaa tcatcaaaat gatgatgaaa atgggtgaaa tggtgacgat caaccaagtt
     8401 atcgaccaag aaaccgcaca attagtggcg gaagaacttg ggcataaagt gattttgcgt
     8461 aaagaaaatg agttagaaga agcggtttta gaagatcgtg atactcacgc agaaaaagta
     8521 acccgtgcac cggtagtgac tattatgggc cacgttgacc acggtaaaac ctcgttactt
     8581 gactacattc gtaaagccaa agtggcggca ggtgaagcag gtggtattac ccagcacatt
     8641 ggtgcatacc acgttgaaac cgatgacggc aagatgatca ctttcttaga taccccgggg
     8701 catgcagcct ttacctcaat gcgtgcgcgt ggtgcgaaag caactgacat cgttgttctc
     8761 gtcgttgcgg ctgatgacgg tgtaatgcca caaactattg aagctatcca gcacgccaaa
     8821 gcagcgggtg cgccattagt ggtagcggtg aataaaattg ataaaccgga agcgaaccct
     8881 gaccgtgtag aacaagagtt actacaacac gaggttattt ctgagaaatt cggtggcgat
     8941 gtgcaattcg tgccggtatc tgcgaaaaaa ggtatgggga tcgatgattt actagaagcc
     9001 attattctgc aatccgaagt gcttgaatta accgcagtaa aagacggtat ggcaagcggc
     9061 gtggtaatcg aatcttactt agataaaggt cgtggccctg tggcaactat cttggttcaa
     9121 tccggtacgc taaacaaagg cgacattgtt ctatgtggtt ttgaatacgg acgtgtgcgt
     9181 gcaatgcgtg atgaaaacgg taaagatgtt gaatcagcag gtccgtctat tccggtagaa
     9241 gtattaggct tatcaggcgt gcctgctgcc ggtgatgagg caaccgtcgt gcgtgatgag
     9301 aagaaagccc gagaagtggc tctttatcgc caaggtaaat tccgtgaagt aaaactggct
     9361 cgtcagcaaa aagctaaact ggaaaatatg ttcagtaata tggcagcagg tgatgtggca
     9421 gaattgaaca tcattgttaa agcagacgta caaggctcgg tagaagcgat cagccaatcc
     9481 ttaatggaac tttcaaccga tgaggtgaaa gtgaaaattg ttggttcagg tgtaggtggt
     9541 attaccgaaa ctgatgcaac cttagccgca gcttctaatg ctattatggt cggctttaac
     9601 gtgcgtgccg atgcctctgc ccgccgtgtg attgaaaacg aaaatatcga tttacgttac
     9661 tactccatca tttatgagct attaaatgac gtgaaagcag caatgagcgg tatgttacag
     9721 cctgaattca aacaagaaat tatcggcttg gcagaagtgc gcgatgtgtt ccgccacccg
     9781 aaatttgggg cgattgccgg ttgtatggtg acagaaggta tcgttaaacg taacaaccca
     9841 atccgtgttc tgcgtgataa cgtagtaatt tttgaaggcg agttagaatc tctccgccgt
     9901 ttcaaagatg acgtatctga ggtgcgtaac ggtatggaat gtggtatcgg cgttaaaaac
     9961 tacaacgacg taaaagttgg cgaccaaatc gaagtgtttg aagtggttga agtaaaacgt
    10021 actatttagc ctaattttgt gtaggggcgt attgcatacg ccctttgtat tttttagggc
    10081 gtgaagcatc acgcccctat aaaagagaaa aatatgtcca gagaatttaa acgtagtgac
    10141 cgtgtggcac aggagctaca aaaagaagtg gcggtgattt tacaacgtga ggtaaaagat
    10201 cctcgcattg gtatggtgac tgtttctgat gtggaagtat caagagattt ggcgtatgcc
    10261 aaaatttttg tgactttctt atttgataac gatgctgaag ctattaagca aggtatgcaa
    10321 ggcttggaga aagcaagccc ctatattcga acccttttag gtaaagcaat gcgtttgcgt
    10381 attgtgcctg aactgcgttt tatctacgat gaatcgctag tcgaaggtat gcgtatgtct
    10441 aatcttgtgt ctagtgtaat caaaaatgac gaagaaaaac ataaagagga ctaattgtgt
    10501 ctagacctcg caaacgtggc cgtgatattc acggcgtttt cttattagat aaaccacaag
    10561 gaatgtcttc aaacgatatt ctgcaaaagg taaagcgaat tttccaagca aataaagccg
    10621 gacataccgg ggcgttagac cctctcgcaa ccggaatgct gccgatctgt ttaggtgaag
    10681 cgaccaaatt ttcgcaattt ttgttggatt cggataaacg ttatctcgtc accgcaaaat
    10741 taggcgagcg aaccgatact tcagatgcgg aagggcaagt ggtggaaacc aaagcagttg
    10801 cagtgacaaa atctcaaatt ttggctgctt tggagaaatt tagaggtgat attttgcaag
    10861 tgccgacaat gttttcggcc ttaaaacaca acggcaaacc cttgtatgaa tatgcccgag
    10921 cagggattac ggtggagcgt gaggctcgcc cgattacgat ttttgaacta aatttcattg
    10981 aatacaaggc tccattttta acccttgagg tgcattgttc taaaggtact tatatccgaa
    11041 ctttggtcga tgatttaggc gaagagttgg gctgtggtgc acacgttacg atgctacgcc
    11101 gtacagcagt ggcagattat ccgattgaaa aaatgatgag ctatgcagat ttgcaaaatt
    11161 tagccgaaaa tcaaccgctt gcagagttag accagcactt attgccgatg gatacggcag
    11221 tttctgcatt agcaaaaata aatttaagtt cagagcaaac taaagcggtg ggttttggtc
    11281 agagaattag atttgataat ctgaaccaac tttatggttt ggtacgttta tattcagaag
    11341 atgaacaatt tctcggtgtg gcagaaatca ccaatgatac tgtgattcgt cctaaccgaa
    11401 tgattaactt aacggaataa gaatgaaatc gacaattttt accttacctt tagcgattat
    11461 cactttagcc ttagccggtt gttcaagtga taatcaagaa agttatgccg gcttaaactt
    11521 agatgctaaa tacagtaaat ctcgctcggt cagtcatttt aatgattatg tgcattttct
    11581 gaaacagaag gcagcagggg caggagtgtc tgcaaaaacc ttaaatgctc agaaattcat
    11641 tcattacaac gccagagcgg tgcagttaga tcaacagcaa gctgcacgaa gacgtgaccc
    11701 gaatgtaccg cctccgccac caaatccaag tggtgtgacc cattatttaa acaaagtatt
    11761 aactcaagcg aaggtggatc aggcaagtga acgttggtat gaatataata gccagcttac
    11821 ccatgccagc caaaaatatg gggtacaaaa agagtatatt ttggcacttt ggggaatgga
    11881 gagcagcttt gggcgtaacc aaggggattt tgatgtactt tcggtgttgg caaccctcgc
    11941 ctttgatggt cgccgtgaga agttatttac gcaagaattt gtgaatgcga tgaaaatgct
    12001 cgatgacggc acaattaaac gctatgaatt gcttggatct tgggcaggag cgatgggaca
    12061 aactcaattt atgccaactt cttacttaac ctatgcagct gatggcaata atgacggtaa
    12121 aaaagatatt tgggagaatg aagccgatgc atttgcctcg attgccaatt atctttcaac
    12181 agtaggttgg gataatcaac taccttgggg tgttgaggtt aaattagctt caccgatgga
    12241 tttaagtttc gccggtcttg aaaccaacaa agcgaaaaca ttggcggaat ggcaagcctt
    12301 aggtatttat ttagcttatc cgaatggaca agaatcggca aaattagctc aattacacgg
    12361 caaagaggtt tggttgattc gtccggataa agaagcggga agagcgtttt tagtttctaa
    12421 taatttccgt actttaatgg attggaataa atcgaataac tttggtatca gtatcggaaa
    12481 atttgccgat cgaattttag cttcagttgg tcaataatcg aataaaagca tagtagcaaa
    12541 gcctatctat gcttttattt tatcaaagcg tcgcctttct tctgcaaacg acgttgctta
    12601 aaaaaatcac tgatcttttg gctacattct ctgcccaata cattgccctg cacttctaag
    12661 aaatggttca ttttataatc ttcaaacaga tgatagcgtg aacctaccgc acctgtttta
    12721 taatcacttg ccccaaaaac caatcgctta atccgactat gtaaaattgc accggcacac
    12781 attgggcaag gctcgagcgt aacataaagg gtacaatcta acagacggta attccctaaa
    12841 gtttgtcctg cttgacgaat tgcctgcatt tcagcgtgtg cagatggatc acagagttga
    12901 atggtttggt tccagcctct gccgataatc tgttggtttt tatcaaccag tactgcccca
    12961 accgggattt cgccttttgc ttcggctaaa tcagcgagat caagggcgta ttgcataaag
    13021 gcgatgtctt gctcggagat ctgaagtgga caagaagttt gatttggttt gataaacata
    13081 gttgcaaaaa attgagtttt tttgaccgct tgtaaaatgc cccacgaggg tggggcatcg
    13141 cttacttcgc ttagtgtcgt ttagtcacga cgaccacgtt tttcactgaa acgtttttct
    13201 ttaaagccac ctttgcggtc tttattgaaa ccaccgtctc ggcgaccacc acgatcatta
    13261 cgatcgttac cacggcgacc acgaccttca aatggatttg cattacttgc actgtttgag
    13321 ccaagtaatg tcatctgcat tggtttgctt aatacacgtg ctttgtgagc gaaatgttgt
    13381 acgagatgat ttggcattcc ttgcggcaac tcaatggttg agtatttatc ataaagtttg
    13441 atatgcccga tgtaacggct gctgatatcc ccctcgttgg caatcgctcc aacaatatga
    13501 cgaacttcca caccgtcttc acgccccaat tcaatgcggt ataaatccat tgccacacca
    13561 ttgttttcgc ggtgttcacg accgttacga cgatcgtttc gctcattgcg gtcacgtcca
    13621 cgttcagaac gggcagcacg cacaaccgga tccggtggca gaatcagttt ttgtttttct
    13681 tgcagtaaca tcatcatcgc cgctgctaat tcttcgtggt cttgatccgc agtaaataaa
    13741 tcttccaaca attcacgata tttttctaaa tcgtgatgtt ctaactgctt agaaatgcgg
    13801 gctttgaatt tctcacggcg tttttccatt aaaatttcgt ggtttggaat cgccacttca
    13861 ttaattggtt ttttgattaa attttcgata ttgcgtaata agcggcgttc acgaggctca
    13921 acaaacagta aggcacgacc tgaacgaccg gcacgaccgg tacgtccgat gcggtgaaca
    13981 taagattctt cttctaacgg aatatcgtag ttaataacca agctgattcg ctcaatatca
    14041 ataccacggg cagcaacatc ggtggcgact aaaatatcta aacgaccgga gcgtaattta
    14101 tctaaggttt gctcacgggc ttgctgggtc atatcgccgt ttaatgccgc agtacggtag
    14161 ccgttgcgtt cacacaattc ggtaatatca atagtaccgg ttttggtgcg ggtaaagatg
    14221 atagcagcat caaattcttc tacttctaag aaacgcaata acgcatcgtt tttacggaag
    14281 ccgtttacca gccagtagct ttgttcaata tccggggctg aacgctcggt tgccttaatt
    14341 tttacctctt gcggatcttt cataaaacga cgggtaatac ggcgaatcgg ctccggcatg
    14401 gtggcagaga agagagcagt ttgatgtgtt tccggtaatt cagccattac cgtttcaaca
    14461 tcgtcaataa agcccatacg cagcatttca tcggcttcat ctaacacgat gaattttaat
    14521 tcggataaat ctaatgtgcc acggcggata tggtcaagaa tacgcccagg tgtacccact
    14581 actacttgtg agccttgttt taaagcacgt aattgaatgt cgtaacgttg tccaccataa
    14641 accgtcacta cacgaatgcc tttcatattt tttgcaaact gctcacaagc atcggctact
    14701 tgaatcgcaa gctcacgggt gggtgccatc accaacattt gcgggtggcg ttgtgttggg
    14761 tcaatttgtg ccaacagcgg cagcgagaat gcggcggttt taccgctccc ggtttgtgcc
    14821 ataccaagca catcacgacc ggctaataaa tgaggaatac attcttgttg aatgggtgat
    14881 gggtttacaa agcccatttc atttacagca tcaaggattg gttgaggtaa gcctaaatcg
    14941 gcgaaagtta aagattcagt tgtcataaaa aaccatttgt ttggaacgga acaaagcaga
    15001 aaatatcgac tatttttctg cacagccatt aacgaaaatt atattgttga aatttaggaa
    15061 gaaatgaaat aacaaagcaa gtgatgcttt aaaaatcccc tgagtataag cggttaaatt
    15121 tacaaaattt tttgcgaaat taaccgcttg tattgtccta atctttccct attctttatc
    15181 aacaacggga gtcgtttgag tcataccacg aatttgtgat aactcaaaca gggcaaaacg
    15241 atactcaacg aagttaaaca cttgatttgc aagagcaaga cgaaagaggc tgattgcctc
    15301 atctttatga tctaatttga gttgttgttt tgctaagtag aaatacgttt ctgtcagtat
    15361 ttctgcatat tgtgagctat tttccttagc gtcacgttcc attatagcac gaacttgagc
    15421 taaattgagc ttccctaaaa aataatttac gatatttgtt ccccagtaat ccgaagaaag
    15481 attattagct cgcttttgta aattattttt agcttctgtg ggtgtaaatt ccagctcatt
    15541 aaaatagagc cataatgctc gataaggatc tttcttatct tcttcataaa aatggagaag
    15601 atcacgctgg gcttcagaat atctgccgct ataatagaaa gccaaacctc gattgagata
    15661 ggtgtagcta tattcccgat ctaattccaa tacggcatta aacgcatcaa tcgaactgtc
    15721 gtaatcgcct tccagcagca gatacagccc catatagtta taagctgccg ccatacgttg
    15781 attgagcgaa atggcttgag caaaatcata acgggctaac gaccataaac ctaaactgtc
    15841 gtaaataacg cctctttcaa aatagaggtc tgcttgctca gagggcgtta atggggcttc
    15901 ttccaatact tggctgagcc ttaccaccat tgcctcttgt tcaaagcgaa gttgaggatt
    15961 aagttctgcc aatgctaagt ttttaattga aaccaaatcg gcataccgac tggtacagcc
    16021 gcttagcatt aaaacagaaa ttaaattcag taataagaaa cggattcgaa acaacttaaa
    16081 aagttgtacc attacagcat atccctcaat taaggaaaat taaaaactga ataacggaca
    16141 ctcaaagtcc gttattcgcc tttattattg aactagaaat tattttcttg ttctgtttct
    16201 accacatttt cagctacagt ttcttctgca tttgcctcgt tggtattatt gatgtctttc
    16261 atcgtcagac gaatgcgtcc ttgacggtca atttctacca ctttcacttg tacttcttga
    16321 ccaacggtta agtagtccgc tacacgctca acacgttcgt tggtgatttg tgaaatgtgg
    16381 actaaacctt ctttgccacc taagatggaa acgaatgcac cgaagtccac cacacgggtt
    16441 actttaccgt tgtagatttg gtttacttcc acttcggcaa cgatctcttc aatacgagcc
    16501 attactgctt tcgctgcatt gccgtcagtt gccgcaattt tcaccgtacc gtcatcatca
    16561 atgtcgatag aagtattggt ctcttcggtt aaagcacgaa ttgttgcacc gcctttacca
    16621 atcacatctt tgattttctt cggatcgatc ttcattgtgt gaatgcgtgg ggcatagtca
    16681 gaaatatctg cacgaggtgc cggaatggct tgttccatta cacctaaaat gtgcattctt
    16741 gcacctttag cttggtttaa ggcaatacgc ataatttcag gggtgatccc ttcgattttg
    16801 atgtccattt gcagcgcagt cacaccggta cgggttcccg ctactttaaa gtccatatcg
    16861 cctaagtggt cttcatcacc taagatgtca gaaagtacca cgaatttctc gtcttctttc
    16921 acgagcccca ttgcgatacc ggcaaccgct gctttgattg gcacacctgc gtccatcaac
    16981 gcaagagacg caccacacac agatgccatt gaagaagaac cgttagattc agtgatttca
    17041 gacaccacac gcactacata cgggaactct tcagcggttg gcatcacggc taatacgcca
    17101 cgttttgcta aacgaccgtg accgatttca cggcgttttg gcgagccgat acgaccggtt
    17161 tcgcccacag agtatggagg gaaattgtag tggaataaga aacggtcaga tttttcgccg
    17221 gtcaattcgt caatgatttg ggcatcacgc tctgtgccca atgttgctac tgctaatgct
    17281 tgggtttcgc cacgggtgaa aagagcagaa ccgtgggtgc gtggtaacac actggtgcaa
    17341 atatccaatg cacgcacggt atccaccgta cggccgtcaa tgcgtggttc gcctgcaata
    17401 atacggctac gcacgatttg gctctctaat gcggtgatga tgtcgatgat agtcccttca
    17461 gaaacagttt catcttctgc ggtaagttgt gcaatcacct ctgctttaat cgcatcgatt
    17521 tgctcgtaac gaacttgttt ttctacgata cgataagcat cgccaaggcg tgtttctgct
    17581 aatgctttta ctttgttgat taaatctgta tttggctctg gtgcaaccca atcccaacgt
    17641 ggtttgcccg cttctttaac aaattctttg aggttttcga ttacaacctg ttgttgctcg
    17701 tggccgaaca ccaccgccgc taacatttgc tcttcggtta agatatccgc ttcagattcc
    17761 accatcaaca cggctttgtc tgtacctgca accactaaat ctaagcggct gattttttgc
    17821 tcgctggtgg ttgggtttaa tacgaattga tcgttgataa aaccgacacg agccgcaccg
    17881 attggaccgt taaacggcac gccggataat gaaagggcag ccgatgcacc gatcatcgca
    17941 accagatcag gactgatttg tgggtttacc gatactacgg tcgcaatcac ttgaatttcg
    18001 ttaaagaaac cttctgggaa aagtggacgc acaggacggt cgatcaagcg agcgattaag
    18061 gtttcacctt cgctaggacg tccttcacgt ttgaagaaac cgcccggaat acgaccggct
    18121 gcgtaagtac gctcttgata atctacggtt aatgggaaga aatcttgccc ttcttttacg
    18181 tcttttttcg ctactacggt aacaaatacg gttgtgtcgt ccatacttgc cattactgct
    18241 gccgttgctt ggcgtgcgat agcaccggtt tctaaggtca cggtgtgctg accgtattta
    18301 aactgtttta caattggagt cattgaaatt ttccttttaa agaatttact attattgtcg
    18361 ttttatttct ctaaattgcg actagcaaaa aggcttttaa gtaaaacatt tttgatagcc
    18421 gcctctcaat aacaagaaat aaaacttgtc tagtatagcg tgatttttat aaaaaatcgc
    18481 ctattatttt gatcttgcac ctcagttata aagaaattca aaacttcagt gtaaaatggg
    18541 gattagtttg tgaaaagcct tgtgaaaata gtttgattgc atcgctattt ttttattaga
    18601 ggtagtttaa aactaaatct gcttccttta ccggcttcac tttcaatttc cagtgtggaa
    18661 tggtgctgtt caagagcgtg tttgacaata gccagaccta aaccgctacc gccggttttg
    18721 ttgctacgag attcgtccac acgatagaaa cgttctgtca gatgggtaat atgatgggca
    18781 tcgataccaa taccgtcatc ttgcacgctg aaaatcgcag tattcggctc ttgattatca
    18841 cgttgacaac atatttgaat ggtaactcct tcaccggcgt gccgtattgc attgtaaatt
    18901 aaatttgata cagcgctttg gagttgctct tgatctcccc ataccgttaa attaggctca
    18961 atatcaaaca gtatttgctg attttggctt aaaatctcgg tatttttttg tatggcggag
    19021 attaacgccg acatctggac ctcattgtgt tcggaatcat tggaatgctc gattttaacc
    19081 aaaagtgaga gttggcgaag caggttagtc atacgttgac tttgctcttt catcgcattg
    19141 agcgcttttt gctgtaatgc catattatgc ggctcgtttt ccagcatttc caaataacct
    19201 tgtaatatgg taagaggcgt tcgcagttcg tggttcatat ttgccaaaaa ggtttgtcgt
    19261 gagtgcataa ggcggacaaa agaggttaca tctcgtgcga caataagtct ggagttactg
    19321 tcatatcgat tgagattcaa ttcaatatag cgtttattat gagtcagcag taccaatggg
    19381 cgatggtgtc gttgttggcg gaaatagctt ttaaattcag gataaaaaat cacattaaaa
    19441 atgcttttat ttgatttttt gtcccaataa aactcaaata gctcttgtgc aacattattg
    19501 caccaagaaa tctccccgtt ttcttggcaa ataatgatcg catcgggtaa gtattgaata
    19561 tgcttattta attttgataa cagacgtaag gttttgattt tatcttggcg taaacgttcg
    19621 tgatagaatg cggcactttg agataaatgc tccattgtcg ttacttttga cctgccgctt
    19681 tgctcattag gtctgagtaa ctgaaggaga gaagattcgg tgtggtgatg ccaaataagt
    19741 agaaccagta aacaaaaact aaaccagaaa aaataagcag ggaaaaacag attgaatacg
    19801 ccggcaacgg ctgctgccag caaaagttcc aataaaaagt gtttaagtga gagcttaact
    19861 ttcattatta gtcctgaaaa tggttggaaa aacggtatcc cgagccacgt acggtttgaa
    19921 tataacgctc aaaaccatag ggttcaagac tcttacgcaa tcgccgaata taagagtcta
    19981 ccgtgcgatc ctcaacataa atatcattac cccaaataaa atcaagcaac tgttcacgac
    20041 gatacacttt ttcagggtga gtaattaaaa agtgaagcag tttatattcc gtgccgctta
    20101 atttcacttc ctgttcggca accaaaaccc gttgtgcctg ttgatcaaga atcaatccgt
    20161 ctatattgat gagattgctt ggcggatcat agcttcgccg ccatactgct tcaatccggg
    20221 caagcaacac ctgtggtgaa aacggtttgg taatgtagtc gtctgcaccg gcattcagac
    20281 cggtaataca gtcttcttcg ctactgcggg ctgttagcat aatgattgga attttttcgg
    20341 tttcaggctg tttttttagg tatttaatta aatggatacc cgaacgcccc ggcatcatcc
    20401 aatccagtaa aataagctcg ggtttttcgc ccagtttatt gatagcactt tggtagtctt
    20461 ccgcttcaat tacctgatat tgtttgctga gatagagact tagcatttca cgaatagcac
    20521 gttcgtcttc aacgatcaga attttgttat tcattcttcc tctccgatgc ggtgtgacaa
    20581 gcggtcgatt tatcgcagaa atttgcaaat tttttactaa aaccgaccgc ttgcagatta
    20641 ccccatttta cctttaatgt aatcttccgt ctgccggaat tttggtttgt cgaaaatttg
    20701 tttggtatca ccgaattcaa tcagctcgcc tagatacata taagcggtat agtcagaaca
    20761 gcgggctgct tgctgcatat tgtgggtaac tatcgctacc gtgtagtcgt gcttgagttc
    20821 gttgataagt tcttcgattt tcatagtcga aatcgggtca agtgcggagc agggctcatc
    20881 cagcagtaac acctcgggtt tcactgcaat acctcgtgca atacagagcc gttgctgttg
    20941 cccgccggag agactgtcac cattttggtt taatttatgc tttacctcat tccataaagc
    21001 cgctttggtt aatgcccact ctacacgatc gtttagctcg gatttcggca atttttcaaa
    21061 cagacgaata ccaaaagcaa cattatcgta aatggacatc gggaatgggg tcggtttttg
    21121 gaaaaccata ccgactttgg ctcgaataat cgaaatatcc gtatcggagg tgagtagatt
    21181 ctctccatca agattgattt cccccgttgc acgttgattc gggtagagct caaacattcg
    21241 gtttagggtt cgcagtaatg ttgattttcc acaaccggaa ggaccgataa atgcggtcac
    21301 tttatttttg gcaatacgca tattgatatt tttcagagcg tggaaatcgc cataataaaa
    21361 attgaggttg ttaatatgta attttgtctc taaatccgtt ggtaacatat agagtcctta
    21421 ttttgttttg ctgaaataca gacgagtgat gatattaaga gaaagcacaa acagagtaat
    21481 aagcattgca cctgcccaag ccaatgtatt ccagtcagcg aacgggctgg ctgcgtattg
    21541 ataaatcacc acagggaggt ttgccattgg tgcattcata tctaaagagg caaactggtt
    21601 ggataatgcg gtaaacaata gcggtgcggt ttctccggaa atacgggcaa cagcgagcaa
    21661 cacgccggta ataatgccgg agcgggcggc acgataagag agcattgtaa ttaccctcca
    21721 tcgtgggcag ccgagagccg cagctgcttc acgcagatta ttaggcacaa gattgagcat
    21781 actgtcggta gttcgaacaa caatcggaat cactaatacc gccaatgcta atgagcctgc
    21841 ccagcctgaa tagtgtttaa cctgtgcgac ataaatcgca taaataaata agccgataat
    21901 aatagaagga gcagagagca acacatcatt caaaaaacgg gtgaattttg ccaatttggt
    21961 ataacgcccg tattcgctca agtaagtgcc ggctaaaata ccgattggtg ttgcaatcag
    22021 tgttgcggta ctgacaatca gtatagagcc gacaattgca ttacgtaatc ctcctgtttc
    22081 atttggtgca ggagtcgggc gggtaaacag ttcccaagac agtgcaggga tacctttttg
    22141 gagtaaagta acaataatcc agaccagcca aaataagcca aataatacag cggaataggc
    22201 aagacttaac atcaagacat tttttatttt tcggcaataa aagcgtcggt tttggtaatt
    22261 tttcattctc gttttccttc ctgtttaccc attcgtgcga ttaataaacg tgaagcggct
    22321 aaaacaatcg aagtaataag gaacagaatt agccctaggc tcatcaacgc tgatttttgt
    22381 aatccgccgg cttcattaaa ttcattggcg atagcagaag cgatggaggt tgaaggggaa
    22441 aataccgaat caggtagcat aaaggcatta ccgatcacaa aggtaactgc cattgtttcg
    22501 cctaatgctc gacctaaccc gagcataatt gcaccgatta ctccggtttt ggtgtaaggt
    22561 agcacgactt tccaaataac ttcccaagtg gttgagccta aaccgtaagc agactctttc
    22621 agtaaaggcg gtacaacact gaaaacatca cgcataattg aggcaataaa gggaataatc
    22681 ataatcgcca ataccaagcc ggcactgaat agcccgatcc cgaaaggagc cccgctaaaa
    22741 agcataccga taaagggaag ctgaccaaat gtatctatca aatacggttg cacatattct
    22801 tgaaataagg gtacaaatac aaacagcccc cacataccgt aaataataga gggaatagcc
    22861 gccagcattt ctaccgctgt gccgacaggg cgttttagcc attgcggtgc aagttcggtt
    22921 aagaaagtcg caatgccgaa cgagactgga acggcaatga ctaatgcaat gccggcagaa
    22981 atcaatgtgc cgataatcgg cactaatgca ccgtatcggt cattcacagg gttccactcg
    23041 ttttcccata ggaatgatac cccgaatcgg cttaatgctt cccaactgcc aagcaataat
    23101 gaaaccagca ccaccaccaa caaggcaaaa accagcaaag caaagaaacg ggttgtgtga
    23161 tgaaataacc cctctaccca aggatggttt aagcatttgt tatcaatttt atgtttatgc
    23221 atagtgacaa ctcttttgca aaatgttgtg aaaaaacgac cgcttgttgt tagttaagct
    23281 ccatacaagc ggtcgttttg cattagtaga gcgaatttcc ttgtccatct ttaacttccg
    23341 ttttccattg agtttggatt ttctctacta cattatccgg tagaggcaca taatccagtt
    23401 cggcagccgc atctttaccg tgcttaaatg cccagtcgaa gaatttaaat acccctttag
    23461 tttgctctgg tttgtcgcta tgttgatgaa tcaagataaa actggcggcg gtaatcggcc
    23521 aggctttttc tcccgcttcg ttggttaaaa taacgcccat accggctgtt tggctccaat
    23581 cggcgttagc agcggcagca gcaaacgttt cacgggaagg ctgaacaaat ttaccatctg
    23641 cattttgcat agaaacccaa gccagagcat tttgtttagc ataggcatat tctacgtaac
    23701 caatagaata cttgagctgt ttcacataag ctgcgacacc ttcatttcct tttccgcctt
    23761 gtccgaccgg ccatttcacc gattttcctt cgccgacttt ctccttccat tcggcagaga
    23821 ctttagaaag atagtttgtc caaccgaaag ttgtgcctga accgtctgag cggcgtacga
    23881 ctaaaattgt ttttgccggc agggcgatat cttgattaag tgcgacaatt gccggatcgt
    23941 tccattgtgt aattttgcct aggaatatat cggcaagaac tttaccggac agtttcagtt
    24001 cgcccgattt aagttcagga atattgacaa ccggtacagt tcccccaatg atggcgggaa
    24061 attgcaaaag
//
LOCUS       DANJWC010000018        23051 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.018, whole genome shotgun sequence.
ACCESSION   DANJWC010000018 DANJWC010000000
VERSION     DANJWC010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 23051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23051
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.018"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            120..470
                     /locus_tag="PWX02_000525"
     CDS             120..470
                     /locus_tag="PWX02_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL1728565.1"
                     /translation="MNTVISQRKPIHWVGSTLDDIREFPDNIKRELGFDLDLVQQGLL
                     PRDFKAMANLGSGVMEIRVKDPSGAFRLVYLAKFKDAIYCLHAFQKKTQKTAPKDIAV
                     IKARYAAVKELENE"
     gene            463..786
                     /locus_tag="PWX02_000526"
     CDS             463..786
                     /locus_tag="PWX02_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL1728566.1"
                     /translation="MNNIDTKIRHTTEAQGNVFEDLGFPIDEAQKLKSASQQLIETKL
                     MLMNEMSNWIDKNNLKQSEAASILGVSRPRISDMVNGKLEKFTLDTLVTFVAKTGKTI
                     QLQLS"
     gene            894..1373
                     /gene="smpB"
                     /locus_tag="PWX02_000527"
     CDS             894..1373
                     /gene="smpB"
                     /locus_tag="PWX02_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDL1728567.1"
                     /translation="MAKKPKVASNTIALNKRARHEYFIEDEIEAGLELQGWEVKALRA
                     GKGNISDSYVIFRHGEAFLFGAMITPLNMASTHIVSDPTRTRKLLLNKRELDSLFGKV
                     NREGYTIVATSMYWKNAWVKVKIGLAKGKKLHDKREDIKDREWQVAKQRIMKNANRG"
     gene            1576..2883
                     /gene="serS"
                     /locus_tag="PWX02_000528"
     CDS             1576..2883
                     /gene="serS"
                     /locus_tag="PWX02_000528"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDL1728568.1"
                     /translation="MIDQNLLRTNLEDVATALKIKRNFILDVERVKTLEEQRKTLQVK
                     TETLQAERNTRSKNIGAAKARGEDISALLAEVDSMGNELDTAKVELDKVQAEIRELLL
                     SVPNLPADEVPLGKDDSENLEVLRWGEPRQFDFEVQDHVTLGENLNGLDFAAGVKLTA
                     SRFVVMKGKLARLHRALSQFMLDLHTEQHGYVETNVPFLVNHDTLYGTGQLPKFGEDL
                     FHTQPLTGQDPNEVQRPFGLIPTAEVPVTNLVRDEIIDEDSLPLRYTAHTPCFRAEAG
                     SYGRDTRGLIRMHQFEKVEMVQIVAPEKSMEALEELTGHAEKVLQLLGLPYRKVLLCT
                     GDMGFGAAKTYDLEVWLPAQNTYREISSCSNMWDFQARRMSARCKAKGDKKTRLVHTL
                     NGSGLAVGRTLVAVLENYQNADGSITVPEVLRPYMGGLEVITA"
     gene            complement(2942..3268)
                     /locus_tag="PWX02_000529"
     CDS             complement(2942..3268)
                     /locus_tag="PWX02_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728569.1"
                     /translation="MVSVDFKNNLLEFIFYGLFSEHTQTNIINGLQFYPKAGVAFRKD
                     LRFRKRAVYQREIMVFYQLDKIDEPKKVTIIAFRLGRMKNPIRLDTVQNRRVVTEIFM
                     NYPEKD"
     gene            complement(3261..3473)
                     /locus_tag="PWX02_000530"
     CDS             complement(3261..3473)
                     /locus_tag="PWX02_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728570.1"
                     /translation="MSIVIVDTDFVVHKEDEEWTTEEIKMIEEHLLEEQSAQYQVKRE
                     VPKQFYADIAERLSRGDLTAWEQLDG"
     gene            3660..6245
                     /gene="leuS"
                     /locus_tag="PWX02_000531"
     CDS             3660..6245
                     /gene="leuS"
                     /locus_tag="PWX02_000531"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDL1728571.1"
                     /translation="MQQQYNPSEIEPKVQRYWAENKVFKAVKDVTKEKYYCLSMLPYP
                     SGKLHMGHVRNYTIGDVVSRYQRMIGKNVLQPMGWDAFGLPAEGAAVKNNTAPAKWTY
                     ENIEYMKGQLKMLGFSYDWDREVTTCRPEYYKWEQWFFTELYKKGLVYKKTSTVNWCP
                     NDETVLANEQVHEGCCWRCDTPVEQREIPQWFIKITDYAEELLNQLDNLPQWPDQVKT
                     MQRNWIGRSEGVEITFKIAGSNVELPVYTTRPDTFFGVSYVAIAAAHPLAELAAQNNP
                     ALAEFIREAKNTKVAEAELATMEKKGMATGLFAIHPLTGKEVPVWVANFVLMHYGTGA
                     VMAVPAHDERDFEFAQKYDLQINQVIQPLDGSEWDFSKAAYTEHGKLINSAEFDGLEF
                     DVAFTAIADKLEAMGVGKRQVNFRLRDWGVSRQRYWGAPIPMMTTEEGEVVTVPLQDL
                     PVILPEDVVMNGVQSPIKADPEWAKTTYNGKPALRETDTFDTFMESSWYYARYTCPQY
                     HEGMLDSDEANYWLPVDQYIGGIEHATMHLLYFRFFHKLLRDAGILNSDEPATKLLCQ
                     GMVLADAFYYTSPTNERIWVSPTQVTLERDEKGRIIKATDPQRRELVHSGMTKMSKSK
                     NNGIDPQEMVEKYGADTVRLFMMFASPAEMTLEWQESGVEGAKRFLGRVWNLVYSYAQ
                     NPATTALDVNALDKDQKELRRDVHKTIAKVSDDIGRRQTFNTAIAAVMELMNKLTKAP
                     LETEQDKAVMAEALSAVVRMLYPITPHICFELWQVLGNEQTIDFAPWVQADESAMVED
                     EKLVVVQVNGKVRGKITVSATATEDEVKAIAKADPNVAKFLDGQQIVKEIYVPFKMLS
                     FAVKA"
     gene            6445..6933
                     /locus_tag="PWX02_000532"
     CDS             6445..6933
                     /locus_tag="PWX02_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728572.1"
                     /translation="MIKKLSLIAVFALTACGWHFKNNEVLPESFRTLTFESADQHSEM
                     SRILRNQLQLSDVKLVPSTANVAKLRLVSTSTNSKVVSVFKQAREAEKILTLNVDAIA
                     DIPNKGQYPLEVSVHRTFFDDSRAALAKSAEKEMIMKDMYEQASRQLIIKMAGLKKEL
                     SK"
     gene            6976..8001
                     /gene="holA"
                     /locus_tag="PWX02_000533"
     CDS             6976..8001
                     /gene="holA"
                     /locus_tag="PWX02_000533"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDL1728573.1"
                     /translation="MQKIFPETLQFSLNKGLQPFYLLTGNDLLLVNETKDAIIHTARL
                     NGFDEKKEVEIKNDTNWEELFEAAQSMGLFFARQIMVLNFPDSPTATQFKKLAELCAF
                     IHSDLLFILHLPKFSKTMKKQAWFTQIELNLVQINCQTPELSKLPQWVSHRAKAMNLQ
                     IENEANQLLCYSYEGNLLALKQALQLLQLNATDGKITLTKAQEVVEHSAQFTPFQWID
                     ALFEGKIQRAQRILYHLQNEEVQAVVLLRIVQKELMILLEITRNPTAVVSSEQSLFIG
                     NLRTEFDRLKIWQNRRPFYNQITQRLTYQKLYALIQQLAELERKVKQEFSDDIWQELE
                     RFSAQFT"
     gene            complement(8002..8187)
                     /gene="yacG"
                     /locus_tag="PWX02_000534"
     CDS             complement(8002..8187)
                     /gene="yacG"
                     /locus_tag="PWX02_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HDL1728574.1"
                     /translation="MPETIVNCPTCEKEVVWSKESKYRPFCSERCQIIDLGDWAAEKH
                     SIASEEAELFSEDLEKY"
     gene            complement(8169..8804)
                     /gene="coaE"
                     /locus_tag="PWX02_000535"
     CDS             complement(8169..8804)
                     /gene="coaE"
                     /locus_tag="PWX02_000535"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDL1728575.1"
                     /translation="MTYVVGLTGGIGSGKTTVSDLFSALGVEVIDADVVARQVVEPGT
                     PLLAKIVEHFGEEILTAEKALERTKLRQIVFNNEQEKTWLNNLLHPAIREEMVKKLQE
                     STACYVIWVVPLLIENKLTEYCDRILVVDVSPEIQLERATKRDNSKIETIKNIMAAQV
                     SREERLSYADDVIENNLPFEQGLLLIKEQVQALHYKYLALAKKEKECQKLL"
     gene            complement(8840..9496)
                     /locus_tag="PWX02_000536"
     CDS             complement(8840..9496)
                     /locus_tag="PWX02_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDL1728576.1"
                     /translation="MLILFSLFIAVWLKAEIPRFAIRVNRQIYQETTSLVNIGLTEQQ
                     FLAQSVLQPKQPRSANLFFLLFPLIMLCSESPIIGTIFILLCFLSLLDMCYYLTDIRY
                     LVLIFLLVLWESLADFQHQTFTFTLAFCLLIGLVSQLMFKKEAFGSGDMLLLVALSPL
                     FRLEKMLLLILTASLLGIGYYLAYWLMKKQKLAKLAFIPFISTAMLLVLMLESRGINY
                     "
     gene            complement(9499..10695)
                     /locus_tag="PWX02_000537"
     CDS             complement(9499..10695)
                     /locus_tag="PWX02_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDL1728577.1"
                     /translation="MTKIYEFHWQAQSRFGQKQRGKRLATNRDMLENLLLAQGYQHIR
                     ISRNFVLSANPKKEEVNSLLSQLALLVNSAMPLKQALSMLLQNCRNIRLYLWLNELIQ
                     HIESGYAFSQSLEKLGEFVTNQEIQLIKMGEQSGKLGIMLTNLAESRAKLEKLAKKVK
                     KILFYPVIVLLISLALSIGLLVLIVPQFAQLYGSKDKTLPLITDILFTLSECLIEQGT
                     VLLGTLLLAVIFYLFFAKKSRLVAKWKMALWAKMPLFSRIIEQSRIIFFSQNIALMLN
                     AHIPLDVALRSFLSEKNDDPILQQEIGTMLNLLQQGYKFSQGINPNVFGAEMAQMLEV
                     GEKSGNVAKMCEHISEMYQQKLDYQIDILSQLLEPMLMLIMGVIVGTIIVGLYLPIFD
                     MGALVE"
     gene            complement(10679..12082)
                     /locus_tag="PWX02_000538"
     CDS             complement(10679..12082)
                     /locus_tag="PWX02_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDL1728578.1"
                     /translation="MEYSVTDLNSQRFIEISAEQWQKNCNEKQILLRYLAVPLQETAE
                     QLWLGIDDMQNLNACEIFAFIYHKQIEPVLISSEELKFLLNALSPEQSSPQSEIYEEN
                     PLYPFSENTEQPDRNDPIIQILENLFKFCLQQNASDIHLEPQKERLLIRLRIDGVLHI
                     YKSLPSSLSSRLISRVKLLAKLDISETRLPQDGQFHFKTLLAETLDFRVSTLPTHFGE
                     KVVLRLQKNKPTHFDFLDLGFNPTQKANLLNALSQPQGLILVTGPTGSGKSITLYSAL
                     SHLNSSEKHILTAEDPIEIEIEGIIQTQVNRGINLDFSQLLRTFLRQDPDIIMLGEIR
                     DEESAEMALRAAQTGHLVLSTLHTNDAPSAVERLLQLGIKEYELKNSLLLVIAQRLLR
                     RVCVKCGGAGCEHCYQGYKGRIGVYQLLNRSAKNFEKNTACLDFKTLADSAKEKLTEG
                     KTNQAEILRVLGNDENL"
     gene            complement(12103..12582)
                     /gene="ppdD"
                     /locus_tag="PWX02_000539"
     CDS             complement(12103..12582)
                     /gene="ppdD"
                     /locus_tag="PWX02_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HDL1728579.1"
                     /translation="MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNS
                     YTQKAALSELLQASASYKSDVELCIYNSGGLDNCSGGTNGIQANKTSSNTTKYLNSIA
                     VTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG"
     gene            12764..14143
                     /gene="radA"
                     /locus_tag="PWX02_000540"
     CDS             12764..14143
                     /gene="radA"
                     /locus_tag="PWX02_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDL1728580.1"
                     /translation="MAKAPKTAYVCNDCGAEYSRWMGQCRECKSWNTISEVRLISSKE
                     TAKSDRFSGYAGETSGKVQTLSDISLQEVPRFSSGFNELDRVLGGGVVPGSAILIGGH
                     PGAGKSTLLLQVMCGLAQHLPTLYVTGEESLQQVAMRANRLGLPTANLKMLSETSVEH
                     ICNIADQEKPKIMVIDSIQVMHLSDIQSSPGSVSQVRECASFLTRYAKTRQVAIIMVG
                     HVTKDGTLAGPKVLEHAIDASLLLEGESDSRFRTLRSQKNRFGAVNELGVFAMTEQGL
                     KEVKNPSAIFLSRSEEQTPGSSVMVLWEGTRPLLVEIQALVDHSMLANPRRVAVGLDH
                     NRLSLLLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALILALISSFRNRPLPQD
                     LVIFGEVGLAGEIRPVTSGQERISEAAKHGFKRAIIPHGNVPKKAIKDMQVFTVKKLS
                     DALDILYNL"
     gene            complement(14198..14428)
                     /locus_tag="PWX02_000541"
     CDS             complement(14198..14428)
                     /locus_tag="PWX02_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HDL1728581.1"
                     /translation="MDYVEILGYVAMILVACSFLLKDVIKLRVVNSLGCACFVVYGLL
                     IGSIPVTGLNALVVCINLYYISKGNRPTATHS"
     gene            complement(14548..15042)
                     /gene="folA"
                     /locus_tag="PWX02_000542"
     CDS             complement(14548..15042)
                     /gene="folA"
                     /locus_tag="PWX02_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HDL1728582.1"
                     /translation="MKISIIVARTINKVIGKDNQMPWHLPVDLAWFRQNTLGKPVIMG
                     RKTYESIGRLLPKRPNIILSRSGFTVEGAYAVQSLEQAVELAKNFANTAEIMIIGGGE
                     LFKQALPLAERLYLTEIQADIEGDTFFEFDEQNWALVEEKWSEIDENNPFRCRFMCLE
                     RKSV"
     gene            15154..16245
                     /gene="proB"
                     /locus_tag="PWX02_000543"
     CDS             15154..16245
                     /gene="proB"
                     /locus_tag="PWX02_000543"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDL1728583.1"
                     /translation="MSKTIVIKFGTSTLTHGTKSLSRPYMLELVKQIAQLHQQHRVIV
                     VTSGAAAAGRDYLGHPELPKTLASKQMLAAVGQSQLIRVWENLFDIYNIHIGQMLLTR
                     ADLDDRERFLNARDTLDALLAQKIIPVINENDAVATEEFKVGDNDNLSALVAILAQAD
                     QLYLLTDQEGLFSADPRSNPDAKLLSVVEKITPEIRQIAGGSSTGLGTGGMSTKITAA
                     DIATRSGVETIIASGSRPNVLVDLANGQNIGSKFLVQSDRLEGRKRWLFAAPQVGEIV
                     VDFGAEQALLQHKSLLPVGVSAIKGEFTRGEVIKIFSQDGKALALGIARYSSEALLRI
                     KGKKSKEIESLLGYEFGSVAVHSDEMVVY"
     gene            16245..17009
                     /locus_tag="PWX02_000544"
     CDS             16245..17009
                     /locus_tag="PWX02_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDL1728584.1"
                     /translation="MELGIDVLAILFAVATLAGFIDAIAGGGGLLTIPALMAAGLPPA
                     MALGTNKLQACGGSLSSSLYFIRKKAVNLKQIWLLILCTFLGSAAGTIFVQMINVDFL
                     KTLLPFLILIIGIYFLVSPKIGDEDRKQRISFTLFAFTAAVALGFYDGMFGPATGSFM
                     TLAFILLLGFNLSKAVAHAKVINFTSNFASLIFFTLGGAVMWKIGLIMMVGQFIGGTL
                     GAKMVMTKGKKLIRPMLVTMSFMMVGKMLYEQGIFN"
     gene            17012..17644
                     /gene="nth"
                     /locus_tag="PWX02_000545"
     CDS             17012..17644
                     /gene="nth"
                     /locus_tag="PWX02_000545"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDL1728585.1"
                     /translation="MNKAKRIEILTRLRNENPKPTTELNYSNPFELLIAVILSAQATD
                     KGVNKATDKLFPVANTPQAILDLGVDGLKEYIKTIGLFNSKAENIIKTCRDLIEKHNG
                     EVPEDRDALEALAGVGRKTANVVMNTAFGHPTIAVDTHIFRVSNRTNFAPGKNVVQVE
                     EKLLKVVPDEFKVDVHHWLILHGRYTCIARKPRCGSCIIEDLCEFKEKTE"
     gene            17839..19089
                     /locus_tag="PWX02_000546"
     CDS             17839..19089
                     /locus_tag="PWX02_000546"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HDL1728586.1"
                     /translation="MDIYLVGGAVRDQLLGLPVKDRDFLVVGATAEQLLAQGFQQVGA
                     DFPVFLHPETKEEYALARQERKNGNGYNGFICDFSPNVTLEQDLIRRDLTINAIAQDE
                     NSTLFDPYGGVQDLENRLLRHVSPAFSEDPLRVLRVARFAARFHSFGFTIAPETLKLM
                     REMVQSGELEHLTAERVWLETQKAFETDNPHVYFDILRLIGALKVLFPELDRLFGVPQ
                     PPQHHPEIDSGKHTLMVLQQAKRLAKKAENPTALLFAALCHDLGKGLTPADILPHHYG
                     HEVKGIQPTRELANRLKIPTEIKDFALLVTEYHTHCHKMAELRPETVIKLFNSLDVWR
                     KPNRFFDFLFACEADARGRLGFEQRNYPQPELAKKYYQAAIEIDVQQVIQDGFEKQAI
                     REELNKRRVYAVRSIKLDSDLINH"
     gene            complement(19190..20125)
                     /locus_tag="PWX02_000547"
     CDS             complement(19190..20125)
                     /locus_tag="PWX02_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDL1728587.1"
                     /translation="MSESKPFPITTGFFAMPLGLGALGLAWLNATPITPYATTIGNII
                     AGTSVILWVALLGCYVYRVFFCFEQIKAEFMHPVQCCLFSLVTMTTMISGDVLYVWGF
                     YLGELLIFLGVMVHLIFLVFIIGGLWKGTQFLPETLHPVHYLPPIAGSFTMASSLSLI
                     GYSEIASLFLGIGIISWLVYEPTMLQRIRLSPLFPIARPSLGIVLAPAFVGASAYFSM
                     GGELDLLAKMLWGYGFLQFCFILRNLKWIAENGFSMGFWAFSFGLAAMARGAIEFYQF
                     EPLSFIGLAGFTIANFGIGLLLIFTLKLWLQQSRA"
     gene            complement(20254..20448)
                     /gene="iscX"
                     /locus_tag="PWX02_000548"
     CDS             complement(20254..20448)
                     /gene="iscX"
                     /locus_tag="PWX02_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HDL1728588.1"
                     /translation="MKWTDTRMIAENLYDMNPDLDPTTVRFTDMHKWICEMEDFDDDP
                     EASNEHILEAILTIWLEEYE"
     gene            complement(20448..20789)
                     /gene="fdx"
                     /locus_tag="PWX02_000549"
     CDS             complement(20448..20789)
                     /gene="fdx"
                     /locus_tag="PWX02_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HDL1728589.1"
                     /translation="MPKIVFLPHEEFCPEGMVVEAKAGDNLLEVAHEAGVEIHHACDC
                     SCACTTCHVVIREGFDSLNDTSDQEEDMLDKAWGLEIDSRLSCQCIVGEEDLVVEIPK
                     YNLNHANEAAH"
     gene            complement(20808..21173)
                     /locus_tag="PWX02_000550"
     CDS             complement(20808..21173)
                     /locus_tag="PWX02_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 51 family protein"
                     /protein_id="HDL1728590.1"
                     /translation="MSNYVKTEISEQFRTVAVEFDLEDNRIFELGERINTEYDVAYMN
                     GYNWAAFLRAYLSIYRPSLLELLEEDPDVGCYYVTYPLSEKSKEKARELKKIIVYLVE
                     NEEEILNFIGEHLDNIEWD"
     gene            complement(21183..23036)
                     /gene="hscA"
                     /locus_tag="PWX02_000551"
     CDS             complement(21183..23036)
                     /gene="hscA"
                     /locus_tag="PWX02_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HDL1728591.1"
                     /translation="MALLQISEPGQTAAPHQHKLAVGIDLGTTNSLVATVRSGQTQVL
                     LDEQERALVPSVVHYAETEKTVGIEAFAKAALDPKNTVISAKRLIGRSLADVQQRYQN
                     LPYQFVASENGLPLIQTAQGNKSPIEVSADILSHLAQFAEKRLAGELSGVVITVPAYF
                     DDAQRQSTKDAARLAGLNVLRLLNEPTAAAIAYGLESGQEGVIAVYDLGGGTFDLSIL
                     RLRKGVFEVLATAGDTALGGDDFDHLLAEWIAEQANYKPQTASEQRELLTLATQTKVA
                     LSQAVEFEVKFANWTGVVSRSQFNELIQPLVKRSLMTCRRALKDAGVEAEEVREVVMV
                     GGSTRVPFVREQVGEFFGKTPLTSIDPDKVVAIGAAVQADILVGNKPDSDMLLLDVVP
                     LSLGIETMGGLVEKIIPRNTTIPVARAQEFTTGKDGQTAMTIHVLQGERELVEDCRSL
                     GKITLRGIPAMAAGVAQVRVTYQVDADGLLNVTAMEKSTKVQASIQIKPSYGLTDEEV
                     TQMIKSSMANAKEDMEARQLVEQRVEADRVIDSVVTALKEDGSAVLSVEEFQAIEAQL
                     ARLIELKEGTARFAIQQGIKEVDLATQEFAARRMNLSIQKALAGKKMDDLA"
BASE COUNT         6906 a         4981 c         4624 g         6540 t
ORIGIN      
        1 attatagcca agcgattcca cacttcagaa tggattcggt gctacaattt ttattggcgg
       61 atatgtttga gtaaaatctt gttgcatatt tccattaaag gtataaaatt ttatacatta
      121 tgaatactgt aatttcccaa cgaaaaccaa ttcactgggt tggatcaacc ttagatgata
      181 ttcgtgaatt tccggataac attaaaagag aactcggttt tgatcttgat ttagttcagc
      241 aaggcttatt accgagggat tttaaagcga tggcaaattt gggttcaggc gtaatggaaa
      301 ttcgagtaaa agatccctca ggggcttttc gcttagtgta tttagctaaa tttaaagatg
      361 caatttactg tttacacgct ttccaaaaga aaacgcaaaa aacagcccct aaagacattg
      421 ctgtcattaa agcacgctat gctgccgtaa aggagttaga aaatgaataa tattgatact
      481 aaaattcgcc atactacaga agctcagggt aatgtttttg aagatcttgg tttccctatt
      541 gatgaagctc aaaaattgaa atctgccagt cagcaactga ttgaaactaa actgatgcta
      601 atgaatgaaa tgagcaactg gattgataaa aataatctca aacaatcaga agcggcttct
      661 attctaggtg ttagccgccc tcgaatttct gatatggtca atggcaagtt agaaaagttt
      721 accctagata ccttagtcac ttttgttgca aaaaccggca aaacaatcca attgcaatta
      781 tcctgatatt tgcaaaattt tactcaaatt caaccgcttg ttgcttttat cattcactaa
      841 caaggttatt tcaagtaaaa ttgtgactaa ttttttatta ataaacacaa attatggcaa
      901 aaaaacctaa agttgcatca aatacgattg cgttaaataa acgtgccaga cacgaatatt
      961 tcattgaaga tgaaattgaa gccggcttag agctacaggg ctgggaagtc aaagcattgc
     1021 gtgccggaaa aggcaatatc agcgatagct atgtcatctt tcgccacggc gaagccttct
     1081 tattcggggc aatgattacg cctttgaata tggcatcaac ccatattgtc agcgacccga
     1141 cccgcacccg caaacttcta ctgaataaac gtgagttaga ctcactgttc ggtaaagtta
     1201 atcgtgaagg ttataccatt gttgccacct caatgtattg gaaaaatgcg tgggtgaaag
     1261 taaaaatcgg cttagccaaa gggaaaaaac tgcacgataa acgtgaagat attaaagacc
     1321 gtgaatggca agtggctaaa cagcgtatta tgaaaaacgc caatcgtggc taaatgaata
     1381 attaccctct ccccttgtgg gagagggaca gaaaaattgc gttcagcaat ttttcaggga
     1441 aagggggatt tgtaaaagtt caaggaaaaa caacgcttgc cctctctctg aaaaatattt
     1501 ctgaacgaaa tatttttcgg tctctctccc ataaagggag agagtgaaac acaaatttaa
     1561 actaaggaac atacaatgat cgaccaaaac ctacttcgta ccaatttaga agacgttgct
     1621 acagctctta aaatcaaacg taattttatt ttagatgtag agcgagtaaa aacccttgaa
     1681 gaacagcgta aaacgctaca agttaaaacc gaaaccttac aagcagaacg caacactcgc
     1741 tccaaaaaca tcggtgcggc aaaagctcgt ggcgaagata tttcagcttt attagccgaa
     1801 gtagattcaa tgggcaacga attagacacg gcaaaagtag aattggataa agtacaggca
     1861 gaaatccgtg agttgttgct ttctgtgcca aacttaccgg cagatgaagt accactcggt
     1921 aaagacgata gcgaaaactt agaagtatta cgttggggcg aaccacgtca attcgacttt
     1981 gaagttcaag atcacgttac ccttggcgaa aacttaaacg gtttagattt tgctgccggt
     2041 gtaaaattaa ccgccagccg ttttgtggta atgaaaggta aacttgcccg cttacaccgt
     2101 gcattatctc aatttatgtt ggatttacac actgaacaac acggctatgt ggaaaccaat
     2161 gtgccatttt tagtcaatca cgacactcta tacggcacag gtcagctacc aaaatttggc
     2221 gaagatttat tccatactca accattaaca gggcaagatc caaacgaggt gcaacgccca
     2281 ttcggtttga ttccaaccgc tgaagtgcca gtaacgaact tagtgcgaga tgaaatcatt
     2341 gatgaagaca gcttaccact gcgttatacc gcccacaccc cttgtttccg tgcggaagcc
     2401 ggttcttatg ggcgtgatac tcgtggttta attcgtatgc accaatttga aaaagtggaa
     2461 atggtgcaaa ttgtcgcccc tgaaaaatca atggaagcct tagaagaatt aaccggtcac
     2521 gcagaaaaag tcttacagct tttaggcttg ccataccgca aagtgctact ctgcactggt
     2581 gatatgggct ttggtgcagc aaaaacctac gacttagaag tttggctacc ggcacaaaac
     2641 acttaccgtg agatttcttc ttgctcaaat atgtgggatt tccaagcccg ccgaatgtct
     2701 gcacgttgta aagctaaagg cgacaagaaa actcgcttgg ttcacacctt aaacggctca
     2761 ggtttagcgg ttggtcgcac actggtcgct gtgttagaaa actaccaaaa tgccgatggc
     2821 tctattactg tgcctgaggt gttaagacct tatatgggtg gtttggaagt aattaccgct
     2881 taagattaat aaatgaaacc aatctcagaa atggggttgg tttttttaat aaatcttatc
     2941 cctaatcttt ttcaggataa ttcataaaaa tttcagtaac tactcggcgg ttttgcactg
     3001 tatctaaacg aataggattt ttcattctcc ctaaacgaaa tgcaataatg gtgacttttt
     3061 tcggttcatc tattttatct aattgataga ataccattat ttcccgttga taaaccgctc
     3121 gctttctgaa acgtaaatcc tttcgaaatg cgacacctgc ttttggataa aactgtaaac
     3181 cattgatgat attcgtttga gtatgctccg aaaataatcc atagaaaata aactctaata
     3241 gattattttt aaaatcaaca ctaaccatct aactgctccc aagcagttaa atctcctcgt
     3301 gataaacgtt cagcaatatc agcataaaat tgtttcggta cttccctttt tacttggtat
     3361 tgagctgatt gttcttctaa taaatgttct tctatcatct tgatttcttc tgttgtccac
     3421 tcttcatctt ctttatgtac aacaaaatcg gtatctacaa tcacaataga catacctagc
     3481 tccttcaagt actggttata tattctgtat tttgcctgtt ctaaattcag ctgtctagct
     3541 tttctgttac tcacaatttg caaatttttc atcaaattcc accgcttgcc attcaaaaaa
     3601 aggcgttttt gcggtatcct atcggcaatt ttttcagtta aatttaaagg ttaaatttta
     3661 tgcaacagca gtataaccca agtgagattg aacctaaggt tcagcgttat tgggcagaaa
     3721 ataaagtttt taaagcagtt aaagacgtta ccaaagagaa atactattgc ctttcaatgt
     3781 tgccttatcc gtcaggtaag ctacatatgg ggcacgttcg taactacacc attggtgatg
     3841 tagtgtctcg ttatcagcgt atgatcggta aaaatgtatt acagccgatg ggttgggacg
     3901 ctttcggttt accggcggaa ggtgctgcgg tgaaaaataa taccgcaccg gcaaaatgga
     3961 cttacgaaaa catcgaatat atgaaaggtc agcttaaaat gttgggcttc tcttatgact
     4021 gggatcgtga agtcaccact tgccgtccgg aatactacaa atgggagcaa tggttcttca
     4081 cagaacttta taaaaaaggc ttagtgtata aaaaaacctc aaccgtaaac tggtgtccga
     4141 atgatgaaac tgtgcttgcc aacgagcaag ttcacgaagg ttgttgctgg cgttgtgaca
     4201 ccccagtgga acagcgtgaa attccacaat ggtttatcaa aatcactgac tatgctgaag
     4261 aattactcaa tcagttagat aatctcccac agtggccgga tcaagtaaaa accatgcaac
     4321 gcaactggat cggtcgttcc gaaggggtag aaatcacctt taaaattgcc ggttcaaacg
     4381 tagaattacc ggtttacact acccgtccgg ataccttctt cggcgtaagc tatgtggcga
     4441 ttgctgcggc tcacccatta gcagaattag ctgcacaaaa taacccggca ttggctgaat
     4501 ttatccgtga agcgaaaaac accaaagtgg cagaagcaga attagccacc atggagaaaa
     4561 aaggaatggc aaccggcttg ttcgccattc acccgttaac aggaaaagaa gtaccggttt
     4621 gggttgctaa ctttgtgtta atgcactacg gtacaggtgc ggtaatggcg gttccggctc
     4681 acgatgagcg tgactttgaa tttgcacaaa aatacgattt gcaaattaat caggtaattc
     4741 aaccgcttga tggctcagag tgggatttca gcaaagccgc ttacaccgaa cacggtaaat
     4801 taatcaactc tgccgagttt gacggcttgg aatttgatgt ggctttcact gcgattgccg
     4861 ataaattaga ggcaatgggc gtaggtaaac gccaagtgaa tttccgtctg cgtgactggg
     4921 gtgtttctcg tcaacgttat tggggtgcac caatcccaat gatgaccaca gaagagggcg
     4981 aagtggttac cgttccatta caagatttac cggtgattct ccctgaagat gtggtaatga
     5041 acggcgtgca aagcccaatc aaagccgacc cagagtgggc gaaaaccact tacaacggca
     5101 aaccggcatt gcgtgaaacc gatactttcg atacctttat ggagtcctct tggtattatg
     5161 cacgttatac ttgcccacaa tatcacgaag gaatgttaga ttcagacgag gcaaactact
     5221 ggctaccggt cgatcaatat atcggcggta ttgaacacgc cacaatgcac ttgctctact
     5281 tccgtttctt ccataaattg ttgcgtgatg cgggtatttt aaattcggac gaaccggcga
     5341 ctaaactgtt atgccaaggt atggtgcttg ccgatgcgtt ctactacacc agcccaacta
     5401 acgagcgtat ttgggtcagc ccgacacaag tcaccttaga acgtgatgag aaaggtcgta
     5461 tcatcaaagc aaccgatcca caaaggcgtg agctggttca cagcggtatg actaaaatgt
     5521 cgaaatcaaa gaacaacggt atcgatccgc aagaaatggt ggaaaaatat ggtgctgaca
     5581 cagttcgctt gtttatgatg tttgcctccc ctgcggaaat gacattagaa tggcaagaat
     5641 ccggcgttga aggggcaaaa cgtttcttag gtcgtgtttg gaatttagtt tatagctatg
     5701 cccaaaatcc ggcaacgact gcattagatg tgaatgcctt agacaaggat caaaaagaac
     5761 ttcgccgtga tgtacacaaa acgattgcta aggtgagtga tgatattggt cgtcgtcaaa
     5821 ccttcaatac agcgattgct gcggtgatgg aattgatgaa caagctcacc aaagccccac
     5881 ttgaaaccga acaagataaa gccgtaatgg cagaagcttt aagtgcggtg gtgcgtatgc
     5941 tttacccaat cacgccacat atctgctttg agttatggca agtgttaggt aatgaacaaa
     6001 ccatcgattt tgccccttgg gtacaagccg atgaatccgc aatggtggaa gatgaaaaat
     6061 tagtggtagt acaggtcaac ggaaaagtgc gtggcaaaat cactgtttca gctactgcta
     6121 cggaagatga agttaaagct atcgctaaag ccgatccaaa cgttgctaaa ttcttagatg
     6181 gtcagcagat tgtaaaagaa atctacgttc catttaaaat gttgagcttt gcggttaagg
     6241 cgtaacaaac acactctccc ttatatggga gagagaccga taaatatttc gtgcagaaat
     6301 atttatcaga gagagggtac aagcggttat tttcttggaa aattttgcaa aaaatagccc
     6361 cctctccctg ctttttcttc tcaacgaaga aaaacctgtc cctctcccac aaggggagag
     6421 gggaataaac aaggaaatct cattatgatt aaaaaactct ctctcatcgc tgtttttgct
     6481 cttaccgcct gcggttggca ctttaaaaac aatgaggtat tacctgaatc gttcagaaca
     6541 ttaacctttg aaagtgcaga tcaacacagc gaaatgtctc gaattttacg caatcagtta
     6601 caactaagcg atgtaaaatt agtgcctagc acggctaatg tggcaaaatt acgtttggtt
     6661 tcaacctcaa caaacagcaa agtggtttct gtgtttaaac aagctcgtga agcagaaaaa
     6721 atcttaacgc taaacgttga tgcgattgcc gatattccaa ataaagggca atacccttta
     6781 gaagtttcgg ttcatcgtac tttctttgat gactctcgtg cagctttggc aaagtctgct
     6841 gaaaaagaga tgattatgaa agatatgtat gaacaagcct ctcgccaatt aattatcaaa
     6901 atggcgggct taaaaaaaga actctcaaaa taatcattgt aggggcgaaa tatgtttcgc
     6961 ccattgttta tctgtatgca aaaaatcttt ccggaaacac ttcaattttc attaaacaaa
     7021 ggactacagc ccttttatct attaacaggg aatgatctgc tattggtgaa tgaaaccaaa
     7081 gatgccatta ttcacaccgc ccgtctcaac ggttttgatg aaaaaaaaga agttgaaatc
     7141 aaaaacgata ctaactggga agaacttttc gaagcagctc aatcaatggg attgtttttt
     7201 gcacgccaaa ttatggtgct gaattttccg gattccccca cagcgacaca gtttaaaaaa
     7261 ctggctgagc tttgtgcctt tattcatagt gatttgctct ttatcctgca cttacctaaa
     7321 ttttccaaaa caatgaaaaa acaggcttgg ttcacgcaaa ttgagctaaa tctcgtgcaa
     7381 attaactgcc aaacaccgga acttagcaaa cttccacaat gggtcagtca tcgagcaaaa
     7441 gcgatgaatt tgcaaattga aaatgaagca aaccaactcc tttgttatag ctacgaaggc
     7501 aatctactgg cgcttaaaca ggcattacaa ctgctacaac tcaatgcaac tgatggaaaa
     7561 atcacgctga caaaagccca agaagtggtt gaacactccg cccaatttac tccatttcaa
     7621 tggattgatg cattatttga aggaaaaatc caaagagctc aacgcatttt gtaccatctg
     7681 caaaatgaag aagtacaagc ggtcgtttta ttaagaattg tgcaaaaaga gttgatgatt
     7741 ttactcgaaa tcactcgcaa cccaaccgca gtcgtttcca gcgaacaatc actttttatc
     7801 ggtaatttac gaacagaatt tgaccgcttg aaaatttggc aaaaccgcag acctttttac
     7861 aaccagatca ctcaacgact gacttaccaa aaactttacg ccctaatcca acaactggct
     7921 gaattagagc gtaaggtgaa acaagaattt agcgatgata tttggcaaga attagagcgt
     7981 ttttccgctc aatttaccta actaatactt ttctaaatct tcgctaaaca actccgcttc
     8041 ttcactggcg atagagtgct tttctgccgc ccaatcgcct aaatcaataa tttgacaacg
     8101 ctcgctacaa aacgggcggt atttactttc tttactccac accacttctt tttcacaagt
     8161 cgggcagttt acaatagttt ctggcattct ttttcctttt ttgctaatgc taagtattta
     8221 taatgtaagg cttgaacctg ctctttaatt aaaagcaagc cctgttcaaa tggcaagtta
     8281 ttttcaatca catcatcagc ataacttaat ctttcttcac ggctgacttg ggctgccata
     8341 atatttttaa tggtttcaat tttactgtta tcacgtttgg ttgctctttc cagttggatt
     8401 tcaggagaaa catcaacaac caatatacga tcacaatatt cggtgagctt attttcaatt
     8461 aaaagcggca cgacccaaat cacataacaa gcggtcgatt cttgcaattt ttttaccatt
     8521 tcttctcgaa tggcggggtg aagtaaatta ttgagccacg ttttttcctg ttcattgtta
     8581 aacacaattt ggcgaagttt agtgcgttct aatgcttttt cagcggttaa gatctcttca
     8641 ccaaaatgct ctacaatctt ggctaaaagc ggtgtacccg gttcaacaac ttggcgagcc
     8701 accacatcag catcaattac ctctactcca agagctgaaa ataaatcaga cactgtggtt
     8761 ttgccactgc ctattccacc ggttaagcct acaacgtaag tcataaaaat tgattcctca
     8821 aaataaaaac aaaccaattc tagtaattaa tccctctgct ttcaagcatt agcaccagca
     8881 acatcgctgt gctaataaac ggaataaacg ccaattttgc taatttctgc tttttcatta
     8941 gccaataagc caagtaataa ccaatcccta gcaggctggc ggttaatatc aataagagca
     9001 ttttttcgag ccgaaagagt ggggataacg caaccaataa caacatatcg cccgagccaa
     9061 atgcctcctt tttaaacata agctgtgaaa ccaagccaat caacaggcaa aaagcaaggg
     9121 taaaggtaaa agtttggtgt tggaaatcgg ctaaactctc ccataacacc aacaaaaata
     9181 taagcacgag gtagcgaata tccgttaggt aatagcacat atccagcagt gataaaaaac
     9241 agagcagaat aaaaatcgtg ccgataatag ggctttccga gcagagcata atcaatggaa
     9301 aaaggagaaa aaatagattt gccgatcggg gttgtttcgg ttgtagcacc gattgagcca
     9361 aaaactgttg ctcggttaag ccaatattca ctaatgaagt tgtttcttgg taaatttggc
     9421 gattgactcg aatggcaaat cgagggatct ccgctttgag ccaaaccgca atgaagaggg
     9481 aaaacagtat taacatcgct actccaccaa agcccccata tcaaaaatcg gtaaatacag
     9541 cccaacaata atagtgccga caatcacgcc cattatcaac attagcatcg gttctaatag
     9601 ctgagacaaa atatcaattt ggtaatctaa tttttgttgg tacatctcac taatatgctc
     9661 acacattttc gccacattgc cacttttctc cccaacctca agcatttgtg ccatctcagc
     9721 accaaacaca ttcgggttga tcccttgtga aaatttgtag ccttgttgta acaaattaag
     9781 catagtgcca atttcttgct gtaaaatcgg gtcatcattt ttctcggata aaaacgacct
     9841 caaggcaaca tcaagcggaa tatgagcatt cagcattaag gcaatatttt ggctgaaaaa
     9901 gataatgcga gattgctcaa taattcggct aaacagtggc attttagccc atagagccat
     9961 tttccacttt gcgactaacc tgctcttttt tgcaaaaaac aggtaaaaaa tgaccgctag
    10021 tagtaacgtg ccaagtaata ctgtgccttg ctcaatcaag cattcggaaa gggtaaataa
    10081 aatatcggta atgaggggta aagttttgtc ttttgagcca taaagttggg caaactgcgg
    10141 tacaatcaaa accaatagcc cgatagataa agcgagcgaa atcagcaaca caatcaccgg
    10201 gtagaacagg attttcttca cttttttcgc caatttttcc aatttcgctc ggctttcagc
    10261 taaattagtc agcataatgc ccagttttcc gctttgttcg cccattttga ttagctgaat
    10321 ttcctgattg gtcacaaatt cacccaattt ctccaacgat tgtgaaaaag catagccact
    10381 ttcaatatgc tgaatcagct cattaagcca tagataaagg cggatatttc ggcagttttg
    10441 caacaacata gataatgcct gttttaacgg catggcagaa ttaaccaaca atgcaagctg
    10501 tgatagcaag ctattcacct cttctttttt cgggtttgct gaaagcacaa aatttcggct
    10561 aatccgaata tgctgataac cttgtgccag caaaaggttt tccaacatgt ctcgattcgt
    10621 tgccaaccgc ttgccccgct gtttttgccc aaatcggctc tgagcttgcc aatgaaattc
    10681 atagattttc gtcattgcct agcaccctta aaatttccgc ctgattggtt tttccttccg
    10741 tcaatttttc ttttgcactg tcggcaaggg ttttaaaatc caaacaagcg gtgttttttt
    10801 caaaattttt tgcagatcgg tttaagagtt gatatacccc aattcgccct ttataccctt
    10861 gataacaatg ctcacagcct gccccaccgc atttcacaca cactcggcgt aacaggcgtt
    10921 gagcaatcac gagtagcaat gaatttttaa gttcatactc ttttatgccg agctgtagca
    10981 accgttctac cgctgaaggg gcatcattgg tgtgcaaagt cgaaagcacc aaatgcccgg
    11041 tttgtgccgc ccgtagtgcc atttctgcac tctcttcatc acgaatttcg ccgagcataa
    11101 taatgtccgg atcttggcgt agaaaggttc ttaacagttg gctgaaatct aagttaatcc
    11161 ctcgattcac ttgggtttgg ataattccct caatctcaat ttcaatcggg tcttcggcag
    11221 tcaaaatatg cttttcactg ctgtttaaat ggcttaatgc actgtaaagc gtgatgcttt
    11281 taccgcttcc tgtcgggccg gttactaaaa tcaatccttg aggctgactc aaagcattca
    11341 gcaaattggc tttctgagtt gggttaaagc ctaaatctaa aaaatcaaaa tgggttggtt
    11401 tatttttttg tagccgtagc accacttttt cgccgaagtg cgttggcaag gtagaaacac
    11461 ggaaatccag cgtttcagct aacagtgttt tgaaatgaaa ttgtccgtct tgtggcaaac
    11521 gagtctcgct gatatcgagc ttagccaata atttaacccg tgaaatcaaa cggctggata
    11581 aactactcgg caggctttta taaatatgta gcacgccatc aattcttaac cgaattaaca
    11641 atctctcttt ttgtggttca agatggatat cggaagcgtt ctgttgcaaa cagaacttaa
    11701 acaaattctc taaaatttgg atgatcggat catttctatc cggctgctcg gtattctcgg
    11761 aaaaaggata aagcggattt tcttcataaa tttcactctg cgggctactc tgttcaggcg
    11821 aaagggcatt gagcaaaaat tttaattctt cggaggaaat caaaacaggc tcaatctgtt
    11881 tgtggtagat aaatgcaaaa atctcgcagg cattaagatt ttgcatatca tcaattccca
    11941 gccaaagctg ttctgctgtt tcttgcagtg gaacggctaa gtaacgcaac aaaatctgct
    12001 tttcattaca atttttctgc cattgttctg ctgaaatctc gatgaatctt tggctgttta
    12061 agtcggttac cgaatactcc attttgattt cctcacctca aattagccgt ttggtgtaac
    12121 agttgtgcta cagaaattag ctggaaatag gctcgtatca ttgccactac aacttgccgt
    12181 ccaactaatc gtattatttg agaacgacgg tgttaaggta tagccataac cattcagaga
    12241 gcctttgccg gttaccgtaa tcacgccttt tgttaccgca attgagttta ggtatttagt
    12301 ggtattacta ctggttttat tcgcctgaat cccattcgtg ccaccggagc agttatccag
    12361 cccgccactg ttgtaaatac agagttcaac gtctgattta tacgaagcag aggcttgcaa
    12421 taactccgat aacgccgcct tttgggtgta tgagttatag gaagggatcg caatggtggc
    12481 aagaatggca ataatcgcaa tcacgatcat caactcaatt agggtaaatg cccgatgcag
    12541 ttggcgaatg ccaagcggtc gaaaatgtac aattttttgc atataaattt ccttataaat
    12601 tgcaccaaca aattgtggcg aatcaagtgt gcaaattttt tgccggcttt gcgaccggca
    12661 aatgtcaatt tttcgatgca gatcgaaaaa ataaattttt catctgttta tccatacagt
    12721 tttttgatag aatttgctaa atttcgtcac tcaaggataa aaaatggcaa aagcacctaa
    12781 aaccgcttat gtttgtaatg attgcggggc tgaatattcc cgttggatgg ggcaatgtcg
    12841 ggaatgtaag tcttggaata ccatcagtga agtgcggttg atttccagca aagaaaccgc
    12901 caaatctgac cgctttagcg gctatgcagg tgaaacttca ggcaaagtac aaacgctgtc
    12961 tgacatcagc ttgcaggaag tgcctcgttt tagcagtggc tttaacgagc tggatcgtgt
    13021 actcggtggc ggtgtggtac cggggtcagc aatactgatt ggtggccacc cgggggcagg
    13081 gaaaagtacg ttattactgc aagtaatgtg cggtttagcc caacatctac caacgcttta
    13141 tgttaccggt gaagagtcgt tacaacaagt tgcaatgcgg gcaaatcgct taggcttacc
    13201 aacggcaaat ctgaaaatgt tgtctgaaac ctctgtcgag catatttgca atattgcaga
    13261 ccaagaaaaa ccgaagatta tggtgattga ctcgattcaa gtgatgcact tgtcggatat
    13321 tcaatcctca ccgggcagtg tttcacaagt gcgtgaatgt gcctctttcc ttacccgcta
    13381 cgccaaaacc cgccaagtgg cgattattat ggttgggcac gtcactaaag atggcacgct
    13441 tgccggcccg aaagtgttgg aacacgccat tgatgcttcc ttgctgttag aaggcgaatc
    13501 cgattcccgt ttccgtactt tgcgtagcca gaaaaaccgc tttggtgccg ttaatgaatt
    13561 gggcgtattt gcgatgaccg agcaagggct aaaagaagtg aaaaacccat ctgcgatttt
    13621 tttaagccgt agcgaagagc aaacgccggg cagttcggtg atggtgttat gggaaggaac
    13681 acgcccattg ttggttgaaa ttcaagcctt agtcgatcac tcaatgctgg caaaccctcg
    13741 ccgtgtggcg gttggcttag atcataaccg tttatcgcta ttacttgcgg tgctacaccg
    13801 acacggtggc ttgcagatgt cggatcaaga tgtgtttgta aatgtggtcg gtggggtgaa
    13861 agtcacggaa accagtgccg atttagcctt aattttggcg ttaatttcca gcttccgtaa
    13921 ccgtccattg ccgcaagatt tggtgatttt tggggaagtc ggcttagcag gggaaatccg
    13981 cccggttacc agtgggcaag agcggatcag cgaagccgca aaacacggct ttaaacgggc
    14041 gataattcct cacggcaatg tcccgaaaaa ggcaattaaa gatatgcagg tgttcaccgt
    14101 gaaaaaactc agtgatgcgt tagatattct gtataatctc taaaatcaag aaccgcttgt
    14161 aagtctgtct tacaagcggt ctgttttagg taaaaattta cgaatgtgtg gcagttggtc
    14221 tattgccttt ggaaatatag tataaattaa tacacaccac caacgcattt aagccggtga
    14281 ccggaatcga gccaatcagc aaaccataaa caacgaaaca ggcacaaccc agcgaattca
    14341 ccacccgcag tttaatcaca tcttttaata aaaaagaaca ggcaactaaa atcatcgcca
    14401 catagccgag tatttcaaca tagtccattt agaacctcct caaataaaat aaactgccga
    14461 tattttaaag gctgtttttt tatttgtatc tagctctaaa aatagcaaag gtttgcgttc
    14521 acatttttac ttttaaccgc ttagttatta aacagatttt cgttctaaac acataaaacg
    14581 gcaacggaaa gggttatttt catcaatttc tgaccacttt tcttctacta acgcccaatt
    14641 ttgttcatca aattcaaaga acgtatcccc ttcaatgtct gcttgaatct cagttaaata
    14701 aagtctttct gctagtggta aggcttgctt aaacagttcc cccccaccaa taatcattat
    14761 ttcagctgta tttgcaaaat ttttcgctaa ttcaaccgct tgttcgagcg attgtaccgc
    14821 ataagcccct tccaccgtaa aacctgaacg ggaaagaata atattagggc gttttggcaa
    14881 taacctgccg atagattcat aggttttccg ccccataatc accggtttgc cgagcgtgtt
    14941 ttggcgaaac caggctaaat ctaccggcaa atgccacggc atttggttat ctttgccaat
    15001 caccttgttg attgttcggg caacaataat gctgattttc attttttctc ctgttatgca
    15061 aattggtttg attttatatc aaattaagcg tgattttaca ccctaaaagt gatacttttg
    15121 gcaacattat ttttagttac aagtgaacag attatgtcaa aaactatcgt gataaaattt
    15181 ggcaccagca ctctcacaca cggcactaaa agtttaagcc gtccttatat gttagagctg
    15241 gtaaaacaaa tcgctcaatt acaccaacag catcgtgtga ttgtggtgac atccggagct
    15301 gccgctgccg gtcgggacta tttaggacac cctgaactcc ctaaaacctt agcctctaag
    15361 caaatgttag cagcggtcgg gcaaagtcag ctcattcgag tgtgggaaaa tttattcgat
    15421 atttataata tccatatcgg gcagatgctc ttaacccgag ccgatctgga cgatcgtgaa
    15481 cgtttcctca atgcccgtga tacccttgat gccctgcttg cacaaaaaat cattccggtg
    15541 attaatgaaa acgatgccgt tgctaccgaa gaatttaaag taggcgataa cgataattta
    15601 tcggctttag ttgcgatttt ggctcaagca gatcagctct atttattaac cgaccaagaa
    15661 ggcttattca gtgccgatcc tcgctcaaac cctgatgcta aattgctctc ggtggtggaa
    15721 aaaatcacgc ctgaaatccg ccaaattgcc ggtggtagtt caaccggttt aggcacgggc
    15781 ggaatgagta ccaaaattac tgctgctgat attgctaccc gctccggtgt ggaaaccatc
    15841 atcgcctccg gttctcgccc aaatgtgtta gttgatttag caaacgggca aaacatcggc
    15901 agtaaatttt tagtccaatc tgaccgcttg gaaggcagaa aacgctggtt atttgctgct
    15961 ccgcaagtgg gtgaaattgt ggtagatttc ggggcggaac aagccttgct tcaacataaa
    16021 tcattactgc cggtgggagt ttctgccatc aaaggcgaat ttactcgagg tgaagtaatc
    16081 aaaattttca gccaagacgg caaagcctta gcactcggca ttgcccgtta tagcagtgag
    16141 gcattgctgc gaattaaagg taagaaatca aaagaaattg aatcgctcct tggttatgaa
    16201 tttggttctg ttgcagtgca tagtgacgaa atggtggtgt attaatggaa ttgggaattg
    16261 atgttttagc aatcttgttt gccgttgcga cccttgccgg ctttatcgat gccatcgctg
    16321 gcggcggtgg cttactcact attccggcat taatggcagc agggcttcct ccggcaatgg
    16381 cacttggcac gaataaatta caggcgtgtg gcggatcact ctcttcctca ctctatttta
    16441 ttcgcaaaaa agctgttaat ttaaaacaaa tttggttgct aattctatgc acttttttag
    16501 gctctgccgc cggtactatt tttgtgcaaa tgatcaatgt cgatttctta aaaacattgt
    16561 tgcctttctt aattctgatt atcggcatct attttttggt cagccctaaa attggcgatg
    16621 aagatcgtaa acaacgcatt agctttaccc tatttgcctt taccgccgct gtcgctctcg
    16681 gtttttatga tggaatgttc ggaccggcaa ccggctcttt tatgactctg gcatttattt
    16741 tgctgctcgg cttcaacctc tctaaagccg tagcacacgc caaagtgatt aattttacct
    16801 ccaactttgc ctcattaatt ttctttacgc ttggtggtgc ggtaatgtgg aaaatcggct
    16861 taattatgat ggtcgggcaa tttattggcg gcacactagg tgcgaaaatg gtgatgacaa
    16921 aaggcaaaaa attaatccgt ccgatgctag tgactatgtc ttttatgatg gtcggcaaaa
    16981 tgctctacga gcaaggaatt tttaattaac aatgaataaa gcaaaacgta ttgagatttt
    17041 aacccgttta cgcaatgaaa atccaaaacc gacaactgaa ctcaattaca gtaatccgtt
    17101 tgaattatta attgccgtga ttctatccgc ccaagcgacc gataaaggcg taaataaagc
    17161 caccgataag ctcttccctg tcgccaacac gccacaagcg attttagatt taggcgtaga
    17221 tgggctaaaa gagtacatca aaactatcgg cttatttaac agcaaggcag aaaatattat
    17281 caagacctgc cgagatctga tcgaaaaaca taacggcgaa gtgcctgaag acagagatgc
    17341 cctcgaagcc cttgccggtg ttggcagaaa aaccgccaat gtggtgatga ataccgcatt
    17401 cggacaccct acgattgcag tcgataccca tattttcagg gtgtcgaacc gcacgaactt
    17461 cgctcccggc aaaaatgtag ttcaggtaga agaaaaactg ctcaaagttg tgccggacga
    17521 atttaaagtc gatgttcacc attggttgat tttacacgga cgctacactt gcattgcccg
    17581 taagcctcgt tgtggttctt gtattattga agatttatgt gagtttaaag agaagacgga
    17641 ataacattat ctggtggtat ttaatgaata actccccttt tagaaaagta ggggctgggg
    17701 gagatttgat actatttcaa catttcgtat gagcgtatag aaattctgcc aaatcttccc
    17761 taccccctct ttgctaaaga ggggaactag atcgagcgta aaatataaca agcggtcatt
    17821 ttttgagatt ttttgcaaat ggatatttat ttagtcggtg gagcagtgcg ggatcaactt
    17881 cttgggctgc cagtgaaaga tagagatttt ttagtggttg gcgcaactgc cgaacaactt
    17941 ttagcccaag gatttcaaca agttggggca gattttccgg tgtttttaca ccctgaaacc
    18001 aaagaagaat atgcccttgc tcgccaagaa cgcaagaacg gcaatggtta caacggcttt
    18061 atttgcgatt tctccccaaa cgtcacgctt gagcaagatt tgatccgccg agatttaacc
    18121 attaacgcca tcgcccaaga tgaaaacagc acactttttg atccttatgg cggtgtgcaa
    18181 gatttagaaa atcggctact ccgccacgtt tctcctgcat tcagcgaaga tccgctacgg
    18241 gtgctacggg ttgcccgctt tgccgcccgt tttcattcat ttggttttac cattgctcct
    18301 gaaaccctga aattaatgcg agagatggta caaagcggtg aattggaaca tttaaccgct
    18361 gaacgtgtgt ggttggaaac acagaaagcc tttgaaaccg ataatccgca cgtttatttt
    18421 gacattttac gcttaattgg ggcattaaaa gtgctattcc ctgaactgga tcgtctgttt
    18481 ggcgtaccac aaccacctca acaccaccct gaaattgact ccggcaaaca cactctaatg
    18541 gtgctacaac aggcaaaaag acttgcaaaa aaagcggaaa atccgaccgc tttactcttt
    18601 gcagcccttt gccacgactt aggcaaaggc ttaacgcctg ctgatatttt gcctcatcac
    18661 tacggacacg aagtcaaagg cattcaacca acccgagagt tagccaatcg gttaaaaatc
    18721 ccaactgaga ttaaagattt tgccctttta gtgaccgaat accacacgca ttgccataaa
    18781 atggcggaac ttcgccctga aacggtaata aaactgttta acagcctaga tgtgtggcgt
    18841 aaaccaaacc gcttttttga tttcctgttc gcctgtgaag cggatgctag agggcgatta
    18901 ggttttgaac agaggaatta cccccaacct gaattggcga aaaaatatta ccaagccgca
    18961 atcgaaattg atgtgcagca agttattcaa gacggttttg aaaaacaagc aattcgggaa
    19021 gagctgaata aacgcagagt gtatgcggta agaagcatca agctagatag cgacctaata
    19081 aaccattagg ttatccgcca ctttctcaaa atcaattccc aaagtttaag ttggttaaat
    19141 gtatggattc tttaaaaggt ggcgttattt tagtgaattt ataatgttcc tatgctcttg
    19201 attgttgtag ccacaatttc agggtaaaga tcagcaataa cccaatgccg aaattggcaa
    19261 tagtgaagcc ggctaaccca ataaaagata aaggttcaaa ttgataaaat tcaatcgcac
    19321 ctcttgccat tgcagctaaa ccaaatgaaa atgcccagaa acccatcgag aagccatttt
    19381 cagctatcca tttgaggttt cgtaaaataa aacagaattg taagaatcca tatccccata
    19441 acatttttgc caacaagtct aattctccac ccatagaaaa ataggcagaa gcgcccacaa
    19501 atgccggagc aagaacaatt cctaaagatg gtctagcaat cggaaaaagc ggactaagcc
    19561 gaattctttg taacatagtg ggttcatata ctaaccagga aattatgcca atgcctaaaa
    19621 ataaagaggc aatttcactg tagccaatta agcttaatga acttgccata gtgaagctcc
    19681 ctgcaattgg cggtaggtaa tgtaccgggt gtagcgtttc cggcaaaaat tgtgtgcctt
    19741 tccataatcc accaataata aaaaccaaga aaattaaatg caccatgacg cctaaaaaaa
    19801 tcaatagttc gcctaaataa aaaccccata catagagcac atcaccgctt atcatggttg
    19861 tcattgtcac taaggaaaat aaacaacatt gcactgggtg cataaattct gctttaatct
    19921 gctcaaaaca aaaaaacaca cgatacacat aacagcctaa taaggctacc caaagtatga
    19981 ctgacgttcc agcaatgata ttaccaattg ttgtagcata aggagtgata ggcgttgcat
    20041 ttaaccaggc aaggcctaac gcccccagcc ctaatggcat tgcaaaaaag cctgtagtta
    20101 ttggaaaagg tttagattca ctcataaatt tatcctcata ttgaaataat gcataactgt
    20161 attcggcttt ataacagtca tatgtaaaaa attttatttt tctaaccgct tgtaataaga
    20221 aaaacctaaa gcacttgctt taggttccta ctactattca tactcttcta gccagatagt
    20281 taaaatcgct tcaagaatat gttcatttga tgcttccgga tcatcatcga aatcttccat
    20341 ttcacaaatc catttatgca tatctgtaaa acgaaccgtg gtcgggtcta aatccgggtt
    20401 catatcatac agattttcag cgatcattcg ggtatctgtc catttcatta gtgcgctgcc
    20461 tcatttgcgt gatttaaatt atattttgga atttcaacaa ccaaatcttc ttcaccaaca
    20521 atacattggc aacttaaacg gctgtctatt tccaatcccc aagctttatc cagcatatct
    20581 tcttcttgat ctgaagtatc atttaacgaa tcaaaaccct cacgaattac cacgtggcaa
    20641 gtggtacaag cacaagaaca gtcgcaagcg tgatgaattt caacgcctgc ttcgtgtgct
    20701 acttctaata aattatctcc ggctttagct tcaacaacca taccttccgg gcaaaattct
    20761 tcgtgtggaa gaaaaacaat ttttggcatt ttaaagtttc cttattacta atcccattca
    20821 atattatcca gatgttcgcc gatgaagttt aaaatctctt cctcattttc aaccagataa
    20881 acaataatct tctttaattc ccttgctttc tctttacttt tctcggaaag cggataggta
    20941 acataataac atcctacgtc cggatcttct tctaataact ctaataggct tggacgataa
    21001 atactaagat aagcccgtaa aaatgcagcc cagttataac cgttcatata ggcaacatca
    21061 tattcagtat taatccgctc gcccaactca aaaatgcggt tatcctctaa atcgaattca
    21121 accgcaacgg ttctgaattg ttctgaaatc tcggttttta catagttaga catactgcct
    21181 ccttatgcta aatcgtccat ttttttaccg gctaacgctt tttgaattga taaattcata
    21241 cgccgtgctg caaattcttg cgtagctaaa tccacctctt tgataccttg ctgaattgca
    21301 aagcgagcag taccttcttt taattcgatt aaacgggcta attgtgcttc aatcgcttgg
    21361 aactcctcca cactcaacac tgcagagcca tcttctttca atgccgttac cacgctgtca
    21421 atcactcggt cggcttctac tcgctgttca actaactggc gggcttccat atcttctttg
    21481 gcatttgcca ttgaggattt aatcatctgc gtaacttctt catcggttaa gccataagac
    21541 ggtttgattt gaattgaagc ctgcactttg gtcgattttt ccattgcagt cacgtttaat
    21601 aaaccgtctg catcaacttg gtaagttacc cgaacctgtg caacccctgc tgccattgcc
    21661 ggaatgccac gcaaagtgat tttaccgaga gagcgacaat cttcgactaa ttcacgctca
    21721 ccttgtaaaa cgtggattgt catcgccgtt tgaccgtctt taccggtagt aaactcttga
    21781 gctcgagcta ctgggatagt agtgttgcgt ggaataattt tctccaccaa accacccatc
    21841 gtttcaatac caagcgaaag tggcacaaca tcaagcaaca acatatcgga atccggtttg
    21901 tttccaacta aaatatccgc ctgcactgcc gcaccaatgg caacaacttt atccgggtcg
    21961 atagaggtta atggcgtttt accgaaaaac tcgccgactt gttcacgcac aaacggtaca
    22021 cgggtcgaac cgcctaccat taccacttca cgaacctctt ctgcttcaac tccggcatct
    22081 ttcaaggctc ggcgacaagt catcagcgag cgttttacaa gcggttgaat tagctcatta
    22141 aattgcgaac ggcttactac gcctgtccaa tttgcaaatt tcacttcaaa ttcaaccgct
    22201 tgcgaaagtg cgactttggt ttgagttgcc aaagttaata attcacgctg ctcgcttgcc
    22261 gtttgaggtt tgtaatttgc ctgttcagca atccattccg ctaataggtg gtcgaaatca
    22321 tcgccaccga gtgcggtatc acctgcggtt gctaatactt caaacacacc tttgcgtaaa
    22381 cgcaaaatag ataaatcgaa agtaccgcca cctaaatcgt aaacggcaat cacaccttct
    22441 tgtccgcttt ctaaaccata agcaatcgct gctgccgttg gttcattgag caaacgtaat
    22501 acatttaaac ccgcaagacg tgcggcatct ttggtacttt ggcgttgtgc gtcatcaaaa
    22561 taagccggta cggtaattac cacgcctgaa agctcaccgg ctaaacgctt ttcggcaaat
    22621 tgtgccaagt ggcttaaaat atccgccgaa acttcaatcg ggcttttatt accttgcgct
    22681 gtttggatta aaggtaaacc gttttcgctc gcgacaaatt gataaggtaa attttgataa
    22741 cgttgctgaa catctgccag agaacgccca atgagacgtt tagctgaaat gacggtattt
    22801 ttcgggtcaa gtgcagcttt ggcaaaggct tcaatcccaa ctgttttttc tgtttccgca
    22861 taatgcacta ctgacggcac taatgctctt tcttgttcat caagcaatac ctgagtctgc
    22921 ccgctacgca cagttgccac taatgaattg gttgtaccta gatcaattcc taccgctaat
    22981 ttatgttgat gtggtgcagc ggtttgaccg ggttcagaaa tttgtaataa tgccatttat
    23041 ttatctcttt a
//
LOCUS       DANJWC010000019        22433 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.019, whole genome shotgun sequence.
ACCESSION   DANJWC010000019 DANJWC010000000
VERSION     DANJWC010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 22433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22433
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.019"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            139..1791
                     /locus_tag="PWX02_000552"
     CDS             139..1791
                     /locus_tag="PWX02_000552"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HDL1728592.1"
                     /translation="MNGARFITESLKAHGVTTLFGYPGGAIMPTYDAIYDSGLDHLLC
                     RNEQGAAMAAIGYARATGKVGVCIATSGPGATNLITGLGDAALDSIPVVAITGQVASH
                     LIGTDAFQEADVLGLSLACTKHSFIVQNIEELPEILALAFQIAQSGRPGPVLVDIPRD
                     VQLAQTSALPIVYPKEPADAQDPNKLVVAKQLLATAKRPVLYVGGGVSMADGVQAVRN
                     FVKIANIPSVSTLKGLGTIDPTDPLYMGMIGMHGTKAANYAVQECDLLIACGARFDDR
                     VTGKLDTFAPHAKVIHIDIDNAEINKLRQAQVALKGNLIEAVNYLAQPLDIEPWREDV
                     RNLKQQFDFQYQENSGDGNIDAVALLHTLSQRKAKNSIVTTDVGQHQMWSAQHLTYHD
                     PKNYITSAGFGSMGFGLPAAIGAIKARAEDQVILITGDGSIMMNIQELGTLKRADLAV
                     KILLLDNQRLGMVRQWQSLFFHGRHSQTILDDNPDFVLLAKAFDIDGERIEKASEVSD
                     AIDRMLNAKGAYLLHVCIPQEDNVWPLVPPGACNADMLDDEV"
     gene            1801..2019
                     /gene="ilvM"
                     /locus_tag="PWX02_000553"
     CDS             1801..2019
                     /gene="ilvM"
                     /locus_tag="PWX02_000553"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HDL1728593.1"
                     /translation="MEEYQLTLKANKRPETLERILRVIRHRGFEVKCLNVESTDSTFN
                     LNFTLASKRPLSLLSHQLEKLFDIIEIN"
     gene            2232..2705
                     /gene="nrdG"
                     /locus_tag="PWX02_000554"
     CDS             2232..2705
                     /gene="nrdG"
                     /locus_tag="PWX02_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDL1728594.1"
                     /translation="MENLRFSSEQIVWQEVPNETSLAFLITGCPLGCKGCHSADSWKA
                     CLGKELSSEYLSERLKRYQGLISCVLFMGGEWEAEKLLPLLQLAKKQGLNTCLYSGLE
                     QHEIAETLLHELTYLKTGRWIAERGGLESLTTNQRFTDLRNNQNLNHLFIRGASI"
     gene            2702..4483
                     /gene="nrdD"
                     /locus_tag="PWX02_000555"
     CDS             2702..4483
                     /gene="nrdD"
                     /locus_tag="PWX02_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDL1728595.1"
                     /translation="MIRLQPTQLDGKLHFINQYIQAKNAADGSKMDANANVTQKNIAT
                     MEQELMKDFFVQINRAKVSEKIAKIFGKETACEYLRQIEAHEIYVHDETSLKPYCVSV
                     TMYPFLRDGLTKLGGESKAPKHLASFCGSFINFVFAVSSQFAGAVATVEFLTYFDYFA
                     RKDYGDNYLQTHRSDIENHLQQVVYSINQPAAARGYQSVFWNISVYDKFYFEAMFSRF
                     VFPDFEKPNWETTSKLQLFFMQWFNKERSKAILTFPVVTAAMLTEKGKCKDDEFANQM
                     AKELSQGNSFFIYQSDNPDSLASCCRLRNEISDHSFSYSLGAGGVATGSINVITINMN
                     RLVQDNRDLSEEVSKIHKYQYAYRKLMEEYLAAGMLPVYDAGFISLHKQFLTIGINGM
                     AEAAESQGLTVGYNPDYIDFVQSRLKIIFQANQAAGEKYGVKFNTEFVPAENLGVKNA
                     KWDKEDGYFVPRDCYNSYFYVVEDEEINALDKFLLHGRELIEWLDGGSALHLNLDEAL
                     TQTGYRSLLDTAAKTGCNYFCINVKITICNECSHIDKRTLHSCSKCGSDNIDYGTRVI
                     GYLKRVSAFSSARQKEHSLRFYHREAA"
     regulatory      4540..4636
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4693..5511
                     /gene="thiM"
                     /locus_tag="PWX02_000556"
     CDS             4693..5511
                     /gene="thiM"
                     /locus_tag="PWX02_000556"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDL1728596.1"
                     /translation="MTTSLPYHSHYLQKIRQNRPLVHNITNIVAANFSANGLLALGAS
                     PIMAAAIEEMESVPALSNAVVINIGTLIGKEVEAMELAGKTANRLGIPLILDPVGVGA
                     TPFRRQTVETLVDQIHFTAIRGNAGELATLAGVEWQAKGVDAGEGSHNVVEIAQQIAK
                     RYQTLVAISGETDVVSSGSHTALLHNGSPMFPKITASGCLLSAVCGAFLAVADKEHYF
                     NALIEACAAYAVAGEVAAQALKPTQYGQFAVNLLDTLGALTPETVEQYVRVEYV"
     gene            5504..6817
                     /locus_tag="PWX02_000557"
     CDS             5504..6817
                     /locus_tag="PWX02_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1728597.1"
                     /translation="MSKTHSNPTKVAVASMVGTAIEYFDNYIYTMAAVLVFNVQFFNS
                     DDPVSNQLMSLSVLALAFFTRPMGSILFGHFGDKYGRKQTLIASLLLMGLSTVAIGLL
                     PNYAAIGIWATVLLCLCRIGQGLGLGGEWGGAALVATEHAPKHKRAFFGIFPQMGAPI
                     GLLLANGAFYLVNLIYGEQTFLDWAWRIPFVASIVMIAIGLYIRIKISESPIFLKAEQ
                     QGKNRHTPLKTLFSQHLKPFMIGVLIATAGYVLFYILVAFTPIFVKSPVAVSQAGHAT
                     GLGLPVNLYTQYLLITSVIFGIAIFFSGIYSDKIGRRLLLLLATGATVVYGLTMPFFL
                     ENGTPTSIFIFLTIGMILIGLSFGPMAVILPELFPTEVRYTGASLAYTAAGILGASVF
                     TIIAVKINENFGLFGVGGYLSVASLLSFWALWQVHETKDLELEEI"
     regulatory      6953..7049
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7117..8646
                     /gene="thiE"
                     /locus_tag="PWX02_000558"
     CDS             7117..8646
                     /gene="thiE"
                     /locus_tag="PWX02_000558"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDL1728598.1"
                     /translation="MSNYSNSPYQPNVIWTVAGSDSCAGAGLQTDLHTFHDFNLVGCS
                     VVTSVTAQHPHGVLCVTPVDDHTFRQQFEALLVQGYPNAIKIGLLCSQAQVEILCEYI
                     QKIRAESSHYCYVVYDPVAVASSGQALSDSILLPIVQQKLYPLVDLITPNGTELALLS
                     DTEIQTFGDVKTASHKLFAQGIKAVLAKGGHFEWLGETVRDYLLTANEIYAFSHARQQ
                     SVNTHGTGCTYASAVGAMLATGFDLADAVTVATAYLQKGLSEKQGRGQTALSSLCHTG
                     YPDDIAFFPQTELISAPLKLPKEPFAPTEYQLGLYPVVDSVKWLERLILVGVKTLQIR
                     IKNRPLAEIEQEIAHSVALAKQHNVRLFVNDYWQLAMKYQAYGVHLGQEDLATADLNA
                     IQQSGLRLGVSTHCYFEIMRALAVKPSYIAFGHVFPTQTKVMPSQPQGLTNLAKYVAL
                     VAPLNIPTVAIGGINEQSIENVMHTGVGSAAVVSAVTQAKDWQQAVKNLAKFANGGRN
                     E"
     gene            8639..9811
                     /gene="cytX"
                     /locus_tag="PWX02_000559"
     CDS             8639..9811
                     /gene="cytX"
                     /locus_tag="PWX02_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HDL1728599.1"
                     /translation="MNRTLSTLNSGLIWAGAGISASAIVVGTWIAPLGWQQGLLAIVL
                     GHLLGGILFFAAGLIGAKTGKNAMQTVQISFGRGSVFFSLANVLQLVGWTAIMIYTGA
                     EISNALSLALWEYSAFSLWAIVLGLLIVLWLFTGSNQVGKFKLVTLIAMFLLTLWLSI
                     KVLNGQENFPVSGEMPFSTAVELATIMPLSWLPLVSDYTAKAKKPFAATLSATIGYLI
                     TSAWMYALGLGMVLFTGQTEIAPMLLMTGMSLIGIMVMILSTVTTTFLDAYSAGVSAG
                     NITAKFKETPTAILVTIIGTLLAISLPVTQYENFLLLIGSVFAPMIAVQIADFFVLKR
                     HQAQPNVDWVAAGLWLVGFVLYHLLSYHQVHLLLGLTIPVMLVIFLSTIFVRRFTK"
     gene            9850..10059
                     /locus_tag="PWX02_000560"
     CDS             9850..10059
                     /locus_tag="PWX02_000560"
                     /inference="COORDINATES: protein motif:HMM:NF015737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL1728600.1"
                     /translation="MESLVLNNDWVVTPQAQLAYHTINGKADEERLNLLVARAGVRVA
                     KGFALNNGWNLQPYAEVNGIAEKGQ"
     gene            10067..10243
                     /locus_tag="PWX02_000561"
     CDS             10067..10243
                     /locus_tag="PWX02_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL1728601.1"
                     /translation="MQVNQYQFGVAENKGRVQGSLGLSAGNGNHRLGLEASVASGKKL
                     KQPFNVLVNYRYQW"
     gene            complement(10284..12140)
                     /gene="sppA"
                     /locus_tag="PWX02_000562"
     CDS             complement(10284..12140)
                     /gene="sppA"
                     /locus_tag="PWX02_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDL1728602.1"
                     /translation="MLTILKAFYRIFRCIRECVINLFFILFILLLVPVVAFISSSGST
                     QKPVFNQGALRLNLDGYLADNREEFTDFYRLVQSELGSSEPFKISTFDVVQAISHAKN
                     DPKITGLVLDLQKLQSADFSSLDFIGNEINNFKQSGKPVIAVGEDYSQKQYYLASFAD
                     EIYLNKVGAVDLKGLNYSNTYFKTLLDKIEAEPHIFRVGTYKSAVEPFMRDDMSEEAK
                     QNARGWLNGTWQQTVATLAQNRNIEPQALDLSPANYIEKYKQAKGDDAQLALNQKWVS
                     QLVSSQESRDKLIAQFGKNSEGSYNQIEFLDYMTELNDRFAEVNAPKIAVVNVEGAIA
                     GGESDEMNVGSETLVKLLQQARDDKNVEGLILRINSPGGSAVASELIRQEVEAFQQAG
                     KPVVASMGGMAASGGYWIAATSDKIIASPNTLTGSIGIFGLAVTFEKTAKNLGVSEDG
                     IATSALAQQAGLKSLPQEQAEVLQIGIENGYDRFLELVSRGRKMPKEVVDKVAQGQVW
                     LGTDALKHGLVDELGNFNVAYNALSELINQKREAENQPKVEQFGLQWFAEQDDSLFGT
                     LARDFKAQLQFSVANWLDLPLLKQPQQQLETLSRFNDPKQMYLYCLNCGKID"
     gene            12248..12811
                     /locus_tag="PWX02_000563"
     CDS             12248..12811
                     /locus_tag="PWX02_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL1728603.1"
                     /translation="MQILDLLHHRRSNKKFGDKAPNAEQLEQILKAGLRVPDHGRLKP
                     YRFVVIEKSGMPQFGECLKSAVAEFDMGEERLKKAEKLANQAPMIIGVVATLDDSIAK
                     VPSWEQMITAGCATYAIQLAANAQGFDTVWITNKWINGSALRSAFGCQESDKIIALIM
                     LGSPAEGEQIALAPESESLEGFVRYLK"
     gene            complement(12882..15206)
                     /locus_tag="PWX02_000564"
     CDS             complement(12882..15206)
                     /locus_tag="PWX02_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDL1728604.1"
                     /translation="MTDLNLKISQIIAGELNVGSHQILAAITLLGEGNTIPFIARYRK
                     EVTGGLDDTQLRHFETRLIYLREMDERRQTILKSIEEQGKLTDELKAKILSSESKTEL
                     EDLYLPYKPKRRTRGQIAIEAGLEPLADSLWNDPKQDPETLAANFVDAEKGVADTKAA
                     LDGARYILMERFSEDAELLAKLRQYLTAYATLESKVIDGKEEEGEKFRDYFAHSEPFN
                     SVPSHRALAMFRGRNEGVLSLSLNADPTAEEGTKSSHCEEIIREHLGVIFNNQPADKW
                     REQVIAWTWKIKAALHLETELMATLREKAEEEAIDVFARNLSALLMAAPAGARNTMGL
                     DPGLRTGVKVAVVDNTGKLLDTATIYPHTGREAEAQLAIFSLIKQHNVELIAIGNGTA
                     SRETERFAKAVIKEIKENKPQTVVVSEAGASVYSASEFAANEFPNLDVSLRGAVSIAR
                     RLQDPLAELVKIEPKAIGVGQYQHDINQSQLARKLDAVVEDCVNAVGVDLNTASAPLL
                     ARVAGMTKTLAQNIVAFRDENGRFTSRSELKKVPRLGPKAFEQCAGFMRIIGGKNALD
                     ASSVHPEAYPVVEKILQATTSTLAELMGNNSKIHSLNAKDFVDEQFGLPTVNDIFKEL
                     EKPGRDPRGEFKTAVFMDGVEEITDLKAGMILEGTVTNVANFGAFVDIGVHQDGLVHI
                     SMLSNSFVEDPHTVVKAGDVVKVKVLEVDAARKRIALTMRLDDKPTDKKEPTSGRNSA
                     EIRKNTQKNDRLQSNNRNAFGNNAFADALKGWKK"
     gene            complement(15206..15760)
                     /gene="pilW"
                     /locus_tag="PWX02_000565"
     CDS             complement(15206..15760)
                     /gene="pilW"
                     /locus_tag="PWX02_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HDL1728605.1"
                     /translation="MFAKFFPKMTACILIFLLAACSSLPPSSSEVEFNRSEAVKARIN
                     LALAYLEQNDFPKAKQNIDKALAHDEKDYLPYSVLAYYYQQTGDAENAEKAYQTALKL
                     SQNRPDVLNNYGTFLCKQGQFQQAYQQFEQAVKSKAPYYHQADSLENMVLCAKKEPNL
                     QKIAETLTELEKLDKPRANLLKEN"
     gene            15980..16231
                     /locus_tag="PWX02_000566"
     CDS             15980..16231
                     /locus_tag="PWX02_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL1728606.1"
                     /translation="MKIVVFQRKALKQLRKIPTGAEIRRKCDDLAYFPYCANIKALTN
                     HQYDYRFRVGNYRIFFNIEGETMNIVSIEEVKKRDERTY"
     gene            16215..16562
                     /locus_tag="PWX02_000567"
     CDS             16215..16562
                     /locus_tag="PWX02_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1728607.1"
                     /translation="MKELTNIQYLNNEEGKPAFAVLPIATLEWLKQKANIKTAIETGI
                     PSAVAGIALDNNYSALRAWREYLGLTQEEVAQRLGISQSAYSQHENAKNLRKTTRIKI
                     AYALGLNEEQLDF"
     gene            complement(16603..17763)
                     /gene="trmG/rlmN"
                     /locus_tag="PWX02_000568"
     CDS             complement(16603..17763)
                     /gene="trmG/rlmN"
                     /locus_tag="PWX02_000568"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HDL1728608.1"
                     /translation="MSELITNAGCGSAEQPKEKINLLNLNRQEMRELFAEMGEKPFRA
                     DQLMKWIYHFGEENFDNMSNINKVLREKLKRIAEIKAPEVAVEQRSADGTIKWAMQVG
                     DQQIETVYIPEDDRATLCVSSQVGCALACTFCSTAQQGFNRNLSVAEIIGQVWRASKI
                     IGNFGVTGVRPITNVVMMGMGEPLLNMNNVIPAMEIMLDDFAYGLSKRRVTLSTSGVV
                     PALDKMREQIDVALAISLHAPNDELRDELVPINKKYNIKMLMDSVNKYLEVSNANHGK
                     VTIEYVMLDHVNDSTDHAHQLAEVLKNTPCKINLIPWNPFPEAPYGKSSNSRVDRFQK
                     TLMEYGFTVTVRKTRGDDIDAACGQLAGDVIDRTKRTLEKRKFGQGIAVKTH"
     gene            18023..18919
                     /locus_tag="PWX02_000569"
     CDS             18023..18919
                     /locus_tag="PWX02_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL1728609.1"
                     /translation="MKLSTIKKALVLTFGLSAAMAMAEPFKVVTTFTVIQDIAQNVAG
                     DKATVESITKPGAEIHDYQPTPKDIVKAQKADLVLWNGMNLERWFERFFENVKGKPAV
                     VVTEGITPMPITEGEYKNLPNPHAWMSTDNALIYIENIRAALVKYDPQNADTYNANAK
                     AYAEKVKGIAEPLRQRLAFIPEQQRWLVTSEGAFSYLAKDYNLKELYLWAINAEEQGS
                     PKQVKKVIDGVKANKIPVVFSESTVSDKPAKQVAKETGALYGGVIYVDSLSEKDGPVP
                     TYLDLLKVTIGTIVEGFERSKK"
     gene            18932..19675
                     /locus_tag="PWX02_000570"
     CDS             18932..19675
                     /locus_tag="PWX02_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL1728610.1"
                     /translation="MTTVSASISVENLSVRYNNGHIALQDVSFHLENGTICALIGVNG
                     GGKSTLFKSLMGLVKPLTGSVKLSQLPIAKALKQNLVSYVPQSEEVDWQFPVSVYDVV
                     MMGRYGYMNFLRRPSQTDKLKVEQAMQRLDVLHLKDRQIGELSGGQKKRVFLARALAQ
                     ESKIILLDEPFTGVDVKTENAIVELLKQLKNEGHLILVSTHNLASVPSFCDQVLMVNR
                     TLLAKGKTEEIFNNQNLERVFGGLLHYQK"
     gene            complement(19732..20541)
                     /locus_tag="PWX02_000571"
     CDS             complement(19732..20541)
                     /locus_tag="PWX02_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL1728611.1"
                     /translation="MLNWILEPLQFEFMQIALITAILVAGVSAVLSCYLVLKGWSLMG
                     DAISHAVLPGVVISSLAGLPLSIGAFFSGLGCALSVGYLKENSRLKEDTVMGIVFSGM
                     FAFGILLFTAFNTGEHLTHILFGNLLGVTKSDLLQTLLISLFTFVVMVAKRKDFLLYC
                     FDVNQAKVAGLPVRLLHYGLLSLLALTIISAMQVVGVILVVAMLITPGITAYLLTKRF
                     DSMLLIALGVAIFSSVFGTLLSYHIDAATGPTIILVQAAIFFLSFAYSKIH"
     gene            complement(20534..21394)
                     /locus_tag="PWX02_000572"
     CDS             complement(20534..21394)
                     /locus_tag="PWX02_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL1728612.1"
                     /translation="MLDLLLEPFTYNYMQKAIFVSMGVGLVCAFLSAFLMLKGWSLIG
                     DALSHAVVPGVAIAYALKLPYAFGAFFSGILAALSILWVKSLTKIKEDAVIGFIFTTF
                     FALGMFIVSLNPTSVDVNSIVMGNILGIADDDLWQVVLIIAISLLGLILFWKDLLLVF
                     FDEHHALSVGLSPLRYKVIFFTLLSACVVVALQTVGAILVIAMVVTPGATAYLLTDRF
                     PRLVLIAMLIGTLTSGIGAYLSYFLDGATGAVIVCLQTLIFLFAFCFAPKYGLITQKR
                     KLHRVEVQNA"
     gene            complement(21468..22253)
                     /locus_tag="PWX02_000573"
     CDS             complement(21468..22253)
                     /locus_tag="PWX02_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HDL1728613.1"
                     /translation="MMKKITILGLGWLGLPLAEALQNKGWRVAGTKRTVGELAIECYP
                     LDLNALAVTSEIEQLLAADALVIALPLSQIRPENYGCGIQTLVSLAIEKGLKHLVFTS
                     STSVLPMKSGVFDENAVVDSANLLVKMENWLLSQPIHCDILRLAGLVGKKRHPVFYLA
                     GKQNLSGAGQPVNLVHLEDCIAAISLLLEQPNGQRIFHLCAEQHPSRKDYYSEMAKRF
                     GLADLHFSEENQPLVRLIKADKICQELGFRYYYPDPYQFKVDI"
     gene            complement(22341..22416)
                     /locus_tag="PWX02_000574"
     tRNA            complement(22341..22416)
                     /locus_tag="PWX02_000574"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22381..22383),aa:Gly,seq:gcc)
BASE COUNT         6448 a         4983 c         4718 g         6284 t
ORIGIN      
        1 aaaaacgact tttatttgtc caaataaatg atggaatcct atttattttt actgaaaata
       61 aagtttaaaa aacagggaat ttatgttatc tatattgcct ggttttaaaa taataaaaat
      121 attaaggaca caacatccat gaatggagca agatttatca ccgagagttt aaaagctcac
      181 ggggtaacga cactttttgg ctatccgggc ggggctatta tgcctaccta tgatgcaatt
      241 tatgattcag gcttagatca tttactttgc cgcaatgaac aaggtgctgc aatggcagct
      301 atcggttatg cccgtgcaac aggtaaagta ggggtttgta ttgcgacctc cggccctggg
      361 gcaactaatt taatcaccgg tttaggcgat gccgcacttg attctattcc tgtggtagca
      421 attaccggac aagttgccag ccatttaatc ggcactgatg ccttccaaga agctgatgta
      481 ctaggtttgt cactcgcttg cactaaacac agttttattg ttcagaatat tgaggagctc
      541 cctgaaattt tagccctcgc attccaaatt gcacaaagtg gcagacctgg tccggtgctg
      601 gtcgatattc cacgtgatgt gcaactagct caaacttcgg ctctgcctat cgtttatcct
      661 aaagaacctg ccgatgcaca agatcccaac aaattagtgg ttgccaaaca attattagcc
      721 accgccaaac gcccggtact ttatgtcggc ggtggcgtta gtatggctga tggtgtacaa
      781 gcggtcagaa attttgtaaa aattgcaaat attccgtctg tttctacctt aaaagggtta
      841 ggcactatcg atccaaccga tccgctctat atgggaatga tcggtatgca cggcacaaaa
      901 gcagcaaatt acgcggtaca agagtgtgat ttactcattg cctgtggtgc ccgctttgat
      961 gaccgtgtta ccggcaaatt agacactttt gcccctcacg ctaaagtgat tcacattgat
     1021 atcgacaatg cggaaatcaa caagctccgt caagcacaag ttgccttaaa agggaatttg
     1081 attgaagccg tgaattactt ggctcaaccg ttagatattg aaccatggcg tgaagatgtg
     1141 cgtaatttaa agcagcaatt tgactttcaa tatcaagaaa atagtggtga tggcaatatt
     1201 gatgccgtgg cgttattaca cacgctttca cagcggaaag ccaaaaacag catcgtcact
     1261 accgatgtag ggcagcatca aatgtggtcg gcacaacatt taacttatca cgatccgaaa
     1321 aactatatca cctctgccgg ttttggctct atgggttttg gcttgcctgc ggcgattggg
     1381 gcaatcaaag ctcgagcgga agatcaggtt attttaatta ccggtgatgg ctcgattatg
     1441 atgaatattc aagaactcgg cacgcttaaa agagctgatt tagcggtcaa aatcctatta
     1501 ctggataacc aacgtttagg tatggttcgc caatggcaat cgctgttctt ccacgggcga
     1561 cacagccaaa ccattttaga tgataaccct gattttgtgt tgttggcgaa agcctttgat
     1621 attgacggtg agcgaattga gaaagcgagc gaagtgtcag atgcaataga cagaatgctc
     1681 aatgccaaag gggcttactt acttcacgtt tgtattccac aggaagataa cgtttggcca
     1741 ctggtgccgc cgggggcgtg taatgccgat atgttagacg atgaggtata ggagaataag
     1801 atggaagaat atcaattaac gcttaaagct aacaaacgcc ctgaaacctt agagcgtatt
     1861 ttacgggtta tacgtcaccg tggttttgag gtgaaatgct taaatgtaga atcaacagac
     1921 agcaccttta acctaaattt tacattagca agtaaacgtc cactctctct tttaagccat
     1981 caattagaaa aactgtttga tatcattgaa atcaattaat cagacttttc attcgccgtg
     2041 taaaaaaata cacggctttt ttatgcttaa aatttatcat tcgctatcct tttgatttta
     2101 ttcatttctt caaatttcaa ctcttttttt tcacttattg ctctttattt tcccattcaa
     2161 cagatctaac atactatata tagtgttaaa taatgttaaa aaacacaata tattgtgtta
     2221 atgaattagt aatggaaaat ctcagattca gctcggaaca aattgtttgg caagaagtgc
     2281 cgaatgagac ctcacttgct tttctgatta caggctgccc gcttggctgt aaaggttgcc
     2341 atagtgcgga tagctggaaa gcctgtcttg ggaaagagct tagcagtgaa tatctctctg
     2401 aacgcctaaa acgctaccaa ggcttaatta gctgcgtatt gtttatgggc ggagaatggg
     2461 aagcagaaaa attactgccg ctgttgcaac tggcaaaaaa acaggggcta aacacctgtc
     2521 tttatagtgg tttggagcag cacgaaatcg ccgagacttt attacacgag ctaacctact
     2581 taaaaaccgg gcgttggatt gccgaacgag gaggattgga aagtttaacc accaaccaac
     2641 gctttactga tttacgcaat aaccaaaatc ttaaccatct ttttattcga ggagcttcaa
     2701 tatgattcgc ttacaaccga cacaattaga cggaaaacta catttcatta accaatatat
     2761 tcaagccaaa aatgccgcag acggttcaaa aatggacgcc aacgctaacg tcacgcaaaa
     2821 aaacatagcg acaatggaac aggagctaat gaaagatttt tttgtgcaaa tcaaccgagc
     2881 aaaagtgagt gaaaaaattg caaaaatttt tggaaaagaa accgcttgtg aatatttacg
     2941 tcaaatcgaa gcacacgaaa tttacgtaca cgatgaaacc agcctaaaac cctactgcgt
     3001 ttcagtcact atgtaccctt tcttgcgtga tggtttgact aaactcggcg gcgaatccaa
     3061 agccccgaaa catttagcct cattctgcgg ttcttttatc aattttgtct ttgcagtcag
     3121 ttctcagttt gccggtgcag tcgccacggt tgaatttctg acctattttg actattttgc
     3181 ccgcaaagat tacggcgata attacttaca gactcatcgt tcagatattg aaaaccacct
     3241 gcagcaggtt gtgtatagca tcaaccaacc ggcagccgct cgtggttatc aaagcgtctt
     3301 ttggaatatt tcagtgtatg acaaattcta ttttgaagcg atgtttagcc gttttgtttt
     3361 tcctgatttt gagaaaccaa attgggaaac cacttcaaaa ttacaacttt tctttatgca
     3421 atggttcaat aaagagcgaa gcaaagcgat tctcactttc cctgttgtga ccgcagcaat
     3481 gcttaccgaa aagggcaaat gtaaagatga cgagtttgcc aatcaaatgg caaaagagct
     3541 atctcaaggc aattcattct ttatttatca atcagataat ccggattccc ttgcttcttg
     3601 ctgccgcttg agaaatgaaa tcagcgatca tagcttctcc tattcattag gggctggagg
     3661 tgttgcgaca ggttcaatca atgtaattac tattaatatg aaccgtttgg tgcaagataa
     3721 ccgtgattta agcgaagaag tcagcaagat ccataaatat caatacgctt accgcaaact
     3781 gatggaagag tatttagctg ccggtatgtt gccggtttac gatgccggtt ttatttcact
     3841 gcataaacaa ttcctaacta ttggcattaa cggaatggca gaagctgctg aatcacaagg
     3901 tttaaccgta ggttacaatc cagactatat tgattttgtg caatctcggt taaaaattat
     3961 tttccaagct aaccaagcag ccggtgaaaa gtatggggta aaatttaata ccgaatttgt
     4021 gcctgctgaa aatttagggg tcaaaaacgc caaatgggac aaagaggacg gctatttcgt
     4081 accacgagat tgctacaatt cctatttcta tgtggtagaa gatgaagaaa ttaatgcttt
     4141 ggataaattc ttactgcacg gcagagagct gattgaatgg ctggacggag gctccgctct
     4201 acacttgaat ttagacgaag ccttaaccca aaccggatat cgctccttgc tggataccgc
     4261 cgctaaaacc ggctgcaatt atttttgtat taacgttaaa atcacgattt gcaacgaatg
     4321 tagccacatc gacaaacgca cgttgcacag ttgtagcaaa tgtggctccg ataatattga
     4381 ttacggcact cgggtaatcg gctatttaaa acgagtctcc gcctttagtt ccgcccgcca
     4441 aaaagagcac agcttgcgtt tctatcaccg agaagcagct taattgaaaa ctgcagttgt
     4501 agccctttac aaccgctttt ttagtgatta tattaaagcc tcatttagtc ggggtgcttg
     4561 cctaagggca agctgagaaa tacccgttga acctgatgca gttagcactg acgtaggaaa
     4621 ctaaaggcgt aaacccatct attccccgtt ggtgttggtg cgttgttttt caatcatatt
     4681 aaggaataaa cgatgaccac atcactcccc tatcactcgc attatttgca aaaaattcga
     4741 caaaatcgac cgcttgttca caacattacc aacattgttg ccgctaattt ttctgccaac
     4801 ggcttattgg ctcttggtgc ttctcccatt atggcagctg ctattgagga gatggaatcc
     4861 gtgccggcac tcagtaacgc cgtggtgatt aatatcggca cgcttatcgg caaagaagta
     4921 gaagcaatgg agcttgctgg caaaaccgcc aaccgtttag gtattccgct cattcttgac
     4981 cctgtcggcg ttggagcaac acctttccgc cgtcaaacag ttgaaacctt agtcgaccaa
     5041 attcacttca ccgccattcg tggaaatgcg ggggaactag ctaccctcgc cggtgtagaa
     5101 tggcaagcca aaggcgttga tgccggcgaa ggctcgcata atgtggttga aatcgcccaa
     5161 cagattgcca aacgttacca aaccttagtc gcaatcagtg gtgaaaccga tgtggtaagc
     5221 tctggctcac acacagcttt attgcacaac ggcagcccga tgttccccaa aattaccgct
     5281 tcaggctgtt tattaagtgc ggtatgcggg gcattcttag ccgttgccga caaagagcac
     5341 tatttcaacg cacttattga ggcttgcgcc gcttatgccg ttgcaggcga agtggctgct
     5401 caagcactca aaccaactca atacgggcaa tttgccgtca atctgctgga tacccttggg
     5461 gcattaacgc ctgaaaccgt tgagcaatat gtgagggttg aatatgtcta aaactcactc
     5521 aaacccaact aaagtggcgg ttgcctcaat ggtcggcacg gcgattgaat attttgataa
     5581 ctacatctac acgatggcag cagtgctggt gtttaatgtg cagtttttta atagcgatga
     5641 tccggtttcc aaccaattaa tgtcgctttc cgtactagct cttgcctttt tcacccgccc
     5701 gatggggtcg attttattcg ggcatttcgg cgataaatac ggacgcaaac aaactctgat
     5761 tgcctcgctc ttattaatgg gcttatctac cgtagcaatc gggcttttgc ctaattatgc
     5821 cgccattggc atttgggcaa ccgtgttact ctgcctctgt agaatcggac aaggtttagg
     5881 cttaggcggc gaatggggcg gagctgctct cgttgccact gaacacgccc cgaaacacaa
     5941 gcgggcattt tttggtattt ttccgcaaat gggcgcccca atcggcttgc tgttggcaaa
     6001 cggagcgttc tacttggtga atttaattta cggtgaacaa accttcttgg attgggcgtg
     6061 gcgaattccg tttgtcgctt ctattgtgat gatcgccatt gggctttata tccgaattaa
     6121 gatcagcgaa agcccgattt tccttaaagc ggaacaacaa ggtaaaaacc gccatacccc
     6181 gcttaaaact ttgtttagcc aacacctcaa gccttttatg atcggcgtgc tgattgccac
     6241 cgccggctat gtgttgttct acattttagt tgctttcacc ccgattttcg tcaaaagccc
     6301 ggtggcagtt tctcaagcag gacacgctac aggcttaggt ttaccggtaa atctctacac
     6361 ccaatatttg ctgattacct ccgtgatttt cggcattgcg attttcttct ccggcattta
     6421 ttcagacaaa atcggacgcc gtttactgct gttacttgcc acaggagcaa ccgtagttta
     6481 cggcttaaca atgccgtttt tcctcgaaaa cggcacaccg acttcgatct ttattttcct
     6541 caccattgga atgatcttaa tcggcttaag cttcggcccg atggcagtaa ttctacccga
     6601 actcttccca acagaagtac gttacacagg ggcttcgctt gcctacaccg ctgccggtat
     6661 tttaggagct agcgtgttca ctattattgc ggtcaaaatc aatgaaaatt tcggcttatt
     6721 cggtgtaggc ggctatttat cggttgcctc attactgagc ttctgggcat tgtggcaagt
     6781 ccacgaaacc aaagatttgg aattagagga gatataaaaa accactcact taacgacggg
     6841 gcaagttacc accttgcccc aagatgaggg aatgcaaatt tccctctaat agacttcaca
     6901 acggatattt tgcatttttt tataaaaaat aaccgcttgt aagctatact aaaaacgctt
     6961 gtcggagtgc cgtaaggctg agatcgcaac agcgagatcc gtggaacctg taggttagta
     7021 cctgcgtagg gaacaagttt tacccaaatt cagattttgg gatcattacc acatctctgg
     7081 caagcaaaac cctaagcata ttatgaggat tttgcgatgt ctaattattc aaactctcca
     7141 taccaaccta acgtcatttg gacggttgcc ggctcagatt cctgtgccgg tgccggtctg
     7201 caaaccgatt tacatacctt tcacgatttt aacctagtgg gttgttcagt agttacttct
     7261 gtaacggctc aacatccgca cggtgtgctg tgtgtaacgc ctgttgatga tcacaccttc
     7321 cgccaacagt ttgaagcctt gctggttcaa ggctacccga acgcaatcaa aatcggctta
     7381 ctctgctcgc aagcacaagt agagatcttg tgtgaatata tccaaaaaat ccgtgcagaa
     7441 agctcgcatt attgttatgt ggtttacgac cctgtggcag tggcaagcag tgggcaggcg
     7501 ttatccgaca gtattttatt gccgatagtg caacaaaaat tgtatccgct ggtggattta
     7561 atcactccaa acggcactga actcgcttta ttaagcgaca cagaaatcca gacctttggt
     7621 gatgtgaaaa ccgctagcca caaactgttt gcacagggaa tcaaagccgt gttagccaaa
     7681 ggtggacatt ttgaatggct gggggaaacg gtaagagatt atctgctgac tgcaaatgaa
     7741 atttatgcct ttagtcacgc ccgacagcaa agtgtgaata cccatggcac aggttgcacc
     7801 tatgcttcag cggttggagc gatgttggca accggctttg atttagctga tgccgtcact
     7861 gttgccaccg cttatttgca aaaaggctta tcggaaaaac aaggcagagg gcaaactgct
     7921 ttaagttcac tttgccacac cggctaccct gatgatatcg ctttcttccc acaaaccgag
     7981 ctgatttctg cccctcttaa attgccgaaa gagccttttg ctccaacgga atatcaactt
     8041 gggctttacc ctgttgtaga tagcgttaaa tggcttgaac gcttaattct tgtaggggta
     8101 aaaaccttgc aaattagaat caaaaatcga ccgcttgcag agattgagca agaaattgcc
     8161 cattcggtcg cactggcgaa acagcacaat gtgcgtttgt ttgtaaatga ttactggcag
     8221 ttagccatga agtatcaggc ttacggcgtg catttagggc aggaggattt agccaccgcc
     8281 gatttaaatg caattcagca atctgggtta cggcttggtg tttcaaccca ctgctacttt
     8341 gaaataatgc gggcattggc ggtgaaaccg tcttatatcg cttttggcca cgttttccct
     8401 actcaaacca aagtaatgcc gtctcaacca caaggcttaa caaatttagc caaatatgtt
     8461 gctctcgttg caccgcttaa cattccaact gtggcgattg gtggaattaa tgagcaaagc
     8521 attgaaaatg taatgcatac cggtgtcggc agtgccgcgg tggtaagtgc cgttacccaa
     8581 gcaaaagatt ggcaacaagc ggtcaaaaac ttagcaaaat ttgcaaatgg aggccgtaat
     8641 gaatagaact cttagtacgc taaacagtgg tttgatttgg gcgggagcgg gcattagtgc
     8701 cagtgcaatc gtggttggca cttggattgc cccacttggc tggcaacaag gcttgctggc
     8761 gatagtgcta gggcatttat taggcggcat tttgtttttt gctgccggct taattggggc
     8821 gaaaaccggc aaaaatgcga tgcaaacggt gcaaatttcc tttggcagag gctcggtgtt
     8881 tttctccctt gccaatgtgc tacagcttgt tggttggaca gccattatga tctatacagg
     8941 ggcagagatc agtaatgccc ttagccttgc cttatgggaa tattcggctt tctcgctttg
     9001 ggcaatcgtg cttgggttgc tgattgtgct atggttattt accggctcta atcaggtcgg
     9061 aaaattcaag ttagtgacat tgatagcgat gtttttgctc accttatggc taagcattaa
     9121 ggtattgaat gggcaggaaa attttcctgt ttcgggtgaa atgccgttta gcaccgcagt
     9181 ggaattagca actattatgc ctctttcgtg gctacctttg gtatcagact ataccgctaa
     9241 agcgaaaaag ccttttgccg cgaccctttc ggcgaccata ggctatttga tcaccagtgc
     9301 gtggatgtat gcgttaggtc tgggtatggt gctatttacc gggcaaaccg aaattgcccc
     9361 tatgttacta atgacaggaa tgagccttat cggcattatg gtgatgattc tttcgactgt
     9421 gactaccacg tttcttgatg cctattccgc cggtgtcagt gccggcaata tcacggcaaa
     9481 attcaaagaa accccaaccg ccattctagt cacaatcatc ggcacgctac tggcaatcag
     9541 tttaccggtt acccaatatg aaaatttcct gctgctgata ggctcggtat tcgccccgat
     9601 gattgccgtg caaattgcgg atttctttgt gttaaaacgc caccaagcac agcctaatgt
     9661 tgattgggtt gctgccggat tatggcttgt cggcttcgtg ctataccacc ttctctctta
     9721 ccaccaagtg catttactac tcggcttaac cattccagtg atgttagtga tttttctcag
     9781 caccattttt gtgaggaggt tcacaaaata gaaagataag cggttcaatt ggagtgaaaa
     9841 tttgctaaaa ttgagtcgct tgttttaaac aatgactggg tagtaacacc acaagctcaa
     9901 cttgcctacc acaccattaa cggtaaagcc gatgaggaac ggttaaatct tttagttgcc
     9961 cgtgccggcg taagagtagc aaaaggcttt gccttgaata atggttggaa tctgcaacct
    10021 tatgcggaag taaacggcat tgctgaaaaa ggccaatagt acgaaagtgc aagtaaacca
    10081 atatcagttt ggagttgccg aaaacaaggg cagagtgcaa ggttcacttg gcttgagtgc
    10141 aggaaacggt aatcaccgtt taggtttaga ggcatcagtt gcttcaggta agaaactgaa
    10201 acaaccattt aatgtattag taaattatcg ttatcaatgg taatttaaga gggcaggtga
    10261 aaacctgccc ttaaattcaa taattaatct atcttcccac aatttaaaca gtaaagatac
    10321 atctgtttcg gatcgttaaa acgtgaaaga gtctctagct gttgttgcgg ttgcttcaac
    10381 agcggtaaat caagccaatt tgccacgcta aattgtaatt gagctttgaa atcacgggca
    10441 agcgtgccaa ataagctgtc atcttgctcc gcaaaccatt gtaagccgaa ctgttcgact
    10501 ttaggttggt tttccgcttc acgtttttgg ttgatcaatt cagataacgc attatatgcc
    10561 acattaaaat tgcccaactc atcaaccaat ccgtgtttta acgcatcagt tcccagccac
    10621 acttgccctt gagccacttt atctaccacc tcttttggca ttttcctgcc acgagaaacc
    10681 agctctaaaa aacggtcgta gccgttttca atcccaattt gcagcacttc tgcctgctct
    10741 tgcggtaggc tttttaaacc ggcttgttgt gctagggcag aagtcgctat gccgtcttcg
    10801 ctcacaccga gatttttagc ggttttttca aaagttactg ctaaaccgaa aataccgata
    10861 gagccggtaa gcgtattcgg gctagcaata attttatcgc tggtagccgc aatccaataa
    10921 ccgcccgatg ctgccattcc gcccattgaa gcaaccaccg gtttgccggc ttgttgaaag
    10981 gcttccactt cttggcgaat cagttcagac gccacagcac tgccgccggg gctgttaatg
    11041 cgtagaatta agccttccac atttttgtca tctcgagctt gttgcagtaa ttttaccagc
    11101 gtctcgctgc ccacattcat ttcgtcactt tcaccgccag caatcgcccc ttccacattc
    11161 actaccgcaa ttttcggagc attcacttct gcaaagcggt cgtttagttc agtcatataa
    11221 tctaaaaact caatctggtt ataactgcct tcgctgtttt tgccaaactg tgcaatcagt
    11281 ttatcccgac tttcttggct ggacactaac tgactaaccc atttttgatt taatgccagt
    11341 tgagcatcat ctcctttagc ttgtttatat ttttcaatgt aattggcagg cgataaatcc
    11401 agtgcttgcg gttcaatgtt gcggttttga gctagtgttg ccacagtttg ttgccaagtg
    11461 ccgtttaacc agcctcgggc attttgtttc gcctcctcag acatatcatc acgcataaac
    11521 ggctcaaccg ccgatttata agtaccgaca cggaaaatat gcggttcagc ctcaatttta
    11581 tcgagcaagg ttttgaaata agtgttggaa taatttaagc cttttaaatc caccgcaccg
    11641 actttattga gataaatttc atcagcaaag gaagcaagat aatattgttt ttgcgagtag
    11701 tcttcgccta ctgcaatcac cggcttgccc gattgtttga agttgttgat ttcattgccg
    11761 ataaaatcca atgagctgaa atcagcactt tgtagctttt gcagatctaa caccaagccg
    11821 gtaattttcg ggtcattttt agcgtggcta atcgcttgca ccacatcaaa agtcgagatt
    11881 ttaaacggct cggaactacc taattccgat tgcaccaagc ggtagaaatc agtgaattct
    11941 tcccgattat ctgctaaata accatctaaa tttaaacgta acgccccttg attgaacacc
    12001 ggcttttgcg tgctgccaga agagctgata aatgcgacca ccggcactaa tagcaaaata
    12061 aacagaataa aaaagagatt aatgacacac tctctaatac aacggaagat tcggtaaaat
    12121 gcttttaaaa tcgttaacat cggaaattcc taaaaataaa ctcagtcggc acagtataaa
    12181 gcaatcgcta aatgaagaaa atatgctatt cttgacttga acgcaaaaaa ttaacaaagg
    12241 aacgcttatg caaatcttgg atttattaca ccatcgccgt tcaaataaaa aatttggcga
    12301 taaagccccg aatgcagaac aactggaaca aattctaaaa gccggcttgc gagtgccgga
    12361 tcacgggcgg ttaaagcctt atcgctttgt ggtgattgaa aaaagcggta tgccacagtt
    12421 tggtgaatgc ttaaagtctg cggtagcaga atttgatatg ggcgaggaac gcttaaaaaa
    12481 agcggaaaaa ttagctaatc aagctccaat gattatcggc gtggtagcaa cattagacga
    12541 cagcatcgcc aaagtgccaa gttgggaaca aatgattacc gcaggctgtg caacttatgc
    12601 gattcagtta gcggcaaatg cacagggctt tgatacggtt tggatcacca ataaatggat
    12661 taacggctct gctcttcgct ccgcttttgg ttgccaagaa tctgataaaa tcattgcctt
    12721 aattatgctt ggctcaccgg cagagggcga gcaaattgca ttagcccctg aaagtgaaag
    12781 tttagagggg tttgtacgtt atcttaagta agcaggttaa cagataaaag ttagtaataa
    12841 gggcgaaaaa catttcgccc ttgcttttat ctgttttcgc cttacttctt ccagcctttt
    12901 aacgcatcgg caaaggcatt gttaccaaaa gcgttgcgat tgttgctttg caagcggtca
    12961 tttttctgcg tatttttgcg aatttctgct gaatttcgac cgcttgttgg ctctttttta
    13021 tcggttggtt tgtcgtctaa acgcatcgta agggcaatac gtttgcgggc agcgtccacc
    13081 tcaagcactt tgactttcac cacatcaccg gctttcacta ccgtgtgcgg atcttcaaca
    13141 aaagagtttg agagcattga aatatgcacc aagccatctt ggtgtacgcc aatatcgaca
    13201 aaagccccaa agtttgccac attggtcact gtgccctcca aaatcatacc ggctttgagg
    13261 tcggtaattt cttccacgcc gtccataaac acggcggttt taaactctcc acgagggtca
    13321 cgccccggtt tttctaactc tttgaaaata tcgttcaccg ttggtaaacc aaactgctca
    13381 tccacaaaat ctttggcgtt gagcgagtga attttgctgt tgttacccat taattctgca
    13441 agtgttgagg tagttgcctg taagattttt tctaccaccg ggtaagcttc cgggtgaacg
    13501 cttgaggcat caagggcatt tttcccgccg ataattcgca taaaaccggc acactgttca
    13561 aaggctttcg gccctaaacg aggcactttt ttcagctctg agcggctggt aaaacggccg
    13621 ttttcgtcac ggaatgccac gatattttgt gccaatgttt tggtcattcc cgccacccga
    13681 gccagcaacg gagcggaggc tgtattcaga tccacgccca ctgcgtttac acaatcttct
    13741 actaccgcat cgagtttacg ggcaagttgg gattggttta tatcgtgttg atactgccca
    13801 acaccaatcg cttttggttc gattttcacc aactcggcaa gtggatcttg caaacggcgg
    13861 gcaatcgaaa ccgctccccg taatgaaacg tctaaatttg gaaactcgtt agccgcaaat
    13921 tcagaggcag aatagaccga tgccccagcc tcactcacca ccaccgtttg cggtttattc
    13981 tctttgattt ctttaatcac cgctttggca aaacgttcgg tttcacgaga ggccgtaccg
    14041 ttaccaatcg caatcagttc cacattgtgt tgtttgatta agctgaaaat cgcaagctgt
    14101 gcctccgcct ctcgcccagt gtgagggtaa attgtggcgg tatccagcaa tttaccggtg
    14161 ttatccacca ccgccacttt cacgccggta cgcaagcccg ggtctagccc cattgtattt
    14221 ctcgcaccgg caggggctgc cattaataat gccgagagat tgcgggcaaa cacatcaatc
    14281 gcctcttctt cagctttttc acgcagggtt gccattaatt cggtttctaa atgcagagct
    14341 gctttgattt tccacgtcca agcaatcact tgctcacgcc atttatctgc tggttgatta
    14401 ttaaaaatca cgcccaaatg ctcacgaata atttcttcac aatggctgga ttttgtgcct
    14461 tcttctgcag tcggatcggc atttaatgaa agtgataaca ccccttcatt tcgcccacgg
    14521 aacatcgcca aagcacggtg ggaaggcacg ctgttaaacg gctcgctatg agcaaaataa
    14581 tcacggaatt tctcgccctc ttcctctttg ccgtcaatca ctttcgattc caacgtggcg
    14641 taagcggtca aatattggcg aagttttgca agtagctcag catcttcgct aaaacgctcc
    14701 attaaaatat atcttgcccc gtctaatgcg gccttggtat ccgcaacgcc tttttccgca
    14761 tcaacaaagt ttgccgctaa ggtttcagga tcttgtttcg gatcattcca taaactgtcc
    14821 gccaatggct ctaaccctgc ttcaatcgca atttgccctc tggtgcgacg ttttggttta
    14881 tacggcaaat agagatcttc cagctcggtc ttgctttcac tgcttagaat tttggctttt
    14941 aattcatcgg ttaatttgcc ttgttcttca atcgacttca aaatggtttg gcgacgttcg
    15001 tccatttcac gcaaataaat taagcgggtt tcaaaatggc gaagttgggt gtcgtctagc
    15061 ccgccggtca cttctttacg gtaacgagca ataaatggaa tggtattgcc ctcacctaat
    15121 agcgtgatgg ctgcaagaat ttggtgtgag ccaacgttga gttcgccggc aataatttgg
    15181 ctgattttta gatttaagtc agtcattagt tttccttcaa taaatttgct cgaggtttat
    15241 ccaatttctc aagctcggtc aatgtttcgg caattttttg cagattcggc tcttttttcg
    15301 cacataacac catattttct aagctatccg cttggtgata gtagggtgct ttgcttttga
    15361 cagcctgctc aaattgctga taagcctgtt gaaattgacc ctgtttacat aaaaaagtgc
    15421 cgtaattatt cagcacatca gggcggtttt gactcaattt cagtgccgtt tggtaagctt
    15481 tttcggcatt ttcagcatca cctgtttgtt ggtaataata ggcaagcacc gaataaggga
    15541 ggtaatcttt ttcatcgtga gccaaggctt tatcaatatt ctgcttagct tttgggaaat
    15601 cattttgctc cagataagct aaagccaaat taatacgggc ttttaccgct tccgagcggt
    15661 taaattcgac ttcagaggaa gaagggggaa gagaagaaca agccgctaat aaaaaaatca
    15721 gaatacaagc ggtcattttt ggaaaaaatt ttgcaaacat aacgttctcc tacaacaaag
    15781 ggcttaattt tactgttcca tcctcgctaa gactagcgat tttttgcgaa gaagaaaaat
    15841 ttttcgtttt catttttcga cgcagatcgc aaaaataata atggggtgtt gtgctagggt
    15901 aaatggtagg aatacactct ctatttaata taagaatttc attgaaaaaa cttataaaac
    15961 ttataaaata gaggcaaaaa tgaaaatagt ggtattccag cgtaaagcct taaagcaatt
    16021 acgaaaaatc ccaactggtg cagaaattcg ccgaaaatgt gatgatttag cttactttcc
    16081 ctactgtgcc aatattaagg cattaacaaa tcatcaatat gattatcgct ttcgggtcgg
    16141 aaattaccgt atttttttca atattgaagg cgaaacgatg aatattgtgt ctatcgaaga
    16201 ggttaaaaaa cgagatgaaa gaacttacta atattcaata tttaaataat gaagaaggaa
    16261 aacctgcctt tgccgtactt ccgatagcaa cacttgaatg gcttaagcag aaagcaaata
    16321 ttaaaaccgc aatagagacc ggtattccat ctgctgttgc tggaattgca ttagataata
    16381 actactcagc tctgcgtgct tggcgggaat atttaggttt aactcaagaa gaggtggcac
    16441 aacgcttggg gatttcgcaa tcagcttata gccaacacga aaatgcaaaa aatctacgaa
    16501 aaacgacccg cataaaaatt gcctatgcgt tgggtttaaa tgaagagcaa ttagatttct
    16561 aataacaaaa aggcaggaac ctctgccttt tcactattca gattaatggg ttttaaccgc
    16621 aataccctgc ccaaatttac gtttttcaag ggtacgttta gtgcggtcaa tcacatcgcc
    16681 ggcaagctgt ccgcaagcgg catcaatatc atcgcctcgt gttttacgca ctgttacagt
    16741 aaaaccgtac tccatcaagg ttttttggaa acgatccacc cgagagttgg agctttttcc
    16801 ataaggtgct tccgggaacg ggttccacgg aatcaagtta attttacacg gcgtgttttt
    16861 caatacttca gcaagttggt gggcgtggtc ggtgctgtca ttaacgtgat ctaacatcac
    16921 atattcaatc gtgaccttgc cgtggttggc gttagaaact tcaaggtatt tattcaccga
    16981 atccatcaac attttgatgt tgtacttttt attaatcggc actaattcat cacgcagttc
    17041 atcgttcgga gcgtgaagtg aaattgccaa cgccacatca atttgctcac gcattttgtc
    17101 taatgccggc accacacctg aagtggaaag cgtgacacga cgtttagata agccgtaagc
    17161 aaaatcatct aacatgattt ccattgccgg aatcacgtta ttcatattca gcaatggttc
    17221 acccattccc atcatgacca cattggtaat cgggcgaaca ccggtcacgc caaaattgcc
    17281 gataatttta gaagcacgcc acacttgccc gataatttcc gccacgctta agttacggtt
    17341 aaagccttgt tgagcggttg agcagaaggt acaagccaag gcacagccta cttgtgaaga
    17401 aacacacagg gttgcacggt catcttcagg aatatatacc gtctcgattt gctgatcgcc
    17461 cacttgcatt gcccatttaa tcgtaccgtc tgcagaacgt tgttctaccg ccacttcagg
    17521 ggctttaatt tcggcaatac gctttaattt ttcacgcaat actttattga tattgctcat
    17581 attatcgaaa ttttcttcgc caaaatggta gatccatttc attagctggt cggcacggaa
    17641 aggtttttca cccatttctg caaacagttc tcgcatttct tgacgattaa gatttaataa
    17701 attgattttt tcttttggtt gttcagcaga cccacagcct gcattggtaa tcagttctga
    17761 cataaagttg aaagcctcgt tattacacgg attacggcaa aaggttaggg caaatgccct
    17821 tattttgcga ttggatttta aagcgtgcaa ttttactggc tcttgaggga ttaggctagt
    17881 aatttttaat caaaagagtt gaattgcctg agtaaactta tcaagaataa ccttataaaa
    17941 acaagactat ttacatcatt cccaatgttc tatagaatag ctttcgataa ttctcattct
    18001 caataacaaa aggaggctaa aaatgaaact atcaacaatt aaaaaagcat tggtattaac
    18061 ctttggttta agtgcagcga tggcaatggc agagccgttt aaagtagtta ccacttttac
    18121 cgtgattcaa gatattgcac aaaatgtcgc cggcgataaa gccactgtgg aatccattac
    18181 taaaccgggt gctgaaattc acgattatca accaacacca aaagacattg taaaagctca
    18241 aaaggcagac ttagtgttat ggaacggtat gaaccttgag cgttggtttg aacgtttctt
    18301 tgaaaatgta aaaggcaaac cggcggttgt cgtcaccgaa ggcatcaccc caatgccgat
    18361 taccgaaggt gaatataaaa atctaccaaa tccgcacgct tggatgtcca ccgataacgc
    18421 attaatttac attgaaaata ttcgtgcggc tttagtaaaa tatgacccac aaaacgcaga
    18481 cacctataat gccaatgcaa aagcctatgc tgaaaaagta aaaggtattg ctgagccact
    18541 ccgccaacgt ttagctttta ttcctgagca gcaacgttgg ttagtaacca gtgaaggggc
    18601 atttagctat ttagcgaaag actacaactt aaaagaactt tatttatggg caatcaatgc
    18661 ggaagagcaa ggttcgccaa aacaagtgaa aaaagtgatt gatggtgtaa aagccaacaa
    18721 aatccctgtt gtgtttagcg aaagtaccgt ttccgacaaa ccggcaaaac aagtggcgaa
    18781 agaaaccggt gcattatacg gtggcgtaat ttatgtggat tcactttcag agaaagatgg
    18841 tccggttcca acctatttag atttgcttaa agtgactatc ggcaccattg tcgaagggtt
    18901 cgaacgaagc aaaaaataat aagatttagc aatgacaact gtttcagcct ccatttccgt
    18961 cgaaaattta tcggtccgtt ataataacgg acatatcgct ttgcaagatg tctcgtttca
    19021 tctggaaaac ggcacaattt gtgcattaat cggcgtgaat ggaggtggaa aatccactct
    19081 gtttaaaagt ttaatggggc tggttaagcc attaaccggc tctgttaagc tgagtcagct
    19141 cccgattgct aaagccctca aacaaaattt ggtttcctat gtgccacaaa gtgaagaagt
    19201 ggactggcaa tttccagttt cggtttacga tgtggtaatg atggggcgat acggctatat
    19261 gaattttcta cgccgcccaa gccaaaccga taagctcaaa gtagagcaag caatgcaacg
    19321 ccttgatgtt ttacatctca aagacaggca aatcggcgaa ctttccggcg ggcaaaaaaa
    19381 acgggtattt ttagcccgtg ccttagctca agaaagtaaa atcatcttac ttgatgagcc
    19441 tttcaccggt gtggatgtta aaaccgaaaa tgcgattgtt gagctactta aacagcttaa
    19501 aaatgagggg catttgattt tagtttccac ccacaacctt gccagcgtac caagcttttg
    19561 cgatcaggtt ttgatggtca atcgcacgct actggcaaaa ggtaaaacgg aagaaatctt
    19621 taataatcaa aatttagaac gggtgttcgg cggtttattg cattatcaaa agtagtgttt
    19681 cagcaacaaa tcacaaacaa atagggaggc aaatgcctcc ctatttttgt atcaatgaat
    19741 cttagaatat gcaaaactga ggaaaaaaat ggccgcttgc acgagaataa tcgtcggccc
    19801 tgttgcggca tcaatatggt agctaagcaa cgtgccgaat accgatgaaa atatcgcaac
    19861 gcctaaggca atcagtaaca tactatcaaa ccgcttagtc agcaggtaag cagtgatccc
    19921 cggggtaatc aacattgcca ccactaaaat cacgcccacc acttgcattg cactaataat
    19981 agttaaagca agcaaggaaa gtaaaccata atgcaataat ctaaccggta agcctgctac
    20041 tttggcttga ttaacatcaa agcaatagag caagaagtct ttgcgtttcg ccaccatcac
    20101 cacaaaggta aagaggctta tgagtaaagt ctgaagcaaa tcgcttttgg ttacgcctaa
    20161 tagattacca aataaaatat gggttaaatg ctcaccggta ttaaaggcgg taaacagcaa
    20221 gatcccaaag gcaaacatac ctgaaaatac aatccccatt accgtgtctt ctttcaagcg
    20281 actattttct tttaaatagc ccaccgataa ggcacagcct aatcctgaaa aaaacgcccc
    20341 tattgagagt ggtaacccgg caagtgagga aatcaccaca ccgggcagaa cagcgtgcga
    20401 aattgcatcg cccattaaag accagccttt cagcaccaga tagcaagata aaaccgcact
    20461 cacgccagca actaaaattg cggtaattaa cgcaatttgc ataaattcaa attgcaacgg
    20521 ctctaaaatc caattaagca ttttgaacct ccacacggtg caatttccgc ttttgagtaa
    20581 tcaagccata tttaggagca aagcagaatg cgaataagaa aattaaggtt tgtaagcaca
    20641 caattaccgc accggttgca ccgtctaaaa agtagcttaa atatgcccca atccctgatg
    20701 tcagcgtgcc gataagcatt gcgatcagca ctaatctagg gaaacggtcg gtaagtaaat
    20761 aagcagttgc tcccggcgtg acgaccatcg caataaccaa aattgcccca accgtttgca
    20821 gagccactac tacacaggca cttaataagg taaagaaaat cactttatag cgtaatggcg
    20881 aaagcccgac tgacaatgcg tggtgttcgt caaaaaagac gagtagcaaa tccttccaaa
    20941 ataggattaa acccagtaac gaaatcgcaa taatcagtac cacctgccac aagtcatcat
    21001 cagcaatgcc taaaatattt cccattacaa tcgaattaac atcaaccgat gttggattga
    21061 gggaaacaat aaacatacca agcgcaaaaa aagtcgtgaa aataaagccg ataaccgcat
    21121 cttctttaat tttcgtcagg ctttttaccc aaagaatcga taatgccgcc aaaatcccgg
    21181 aaaaaaacgc accaaaggcg tagggcaatt ttagtgcata agcgatggca acacctggca
    21241 ctacagcgtg tgataaggca tcaccaatca aagaccagcc ttttaacatc aaaaaggcgg
    21301 aaagaaaagc acaaactaac cccacgccca tactgacaaa aatcgctttt tgcatatagt
    21361 tgtaggtaaa aggctctaat aaaagatcaa gcataaaatc ctaacaagtt gttgataata
    21421 gttcgcatat actatcatat aaactccttt tcttgtatgg attaaactta aatatctact
    21481 ttaaattgat aaggatcagg atagtaataa cgaaaaccaa gctcttggca aattttgtct
    21541 gcttttatca gccttacaag cggttgattt tcttcagaaa aatgcaaatc cgctaaccca
    21601 aaacgttttg ccatttcact ataataatct ttacggcttg ggtgctgttc ggcacataaa
    21661 tgaaaaatac gctgcccgtt cggctgctcc aacagcaatg aaatggctgc aatacagtcc
    21721 tctaaatgca ctaaattgac cggctgccct gctccactca agttctgttt tcccgccaaa
    21781 taaaaaaccg ggtgccgttt tttgcccacc aagccagcta aacgcaaaat atcacaatga
    21841 attggctggc tcagcaacca attttccatt tttactaata ggttagcaga atcaaccact
    21901 gcattttcat caaaaacgcc cgatttcatt ggcaaaaccg aagtcgagct ggtgaaaacc
    21961 agatgtttca agcccttttc tattgcaagc gaaactaatg tttgaatacc gcaaccgtaa
    22021 ttttcagggc gaatttggct aaggggcaag gcaatcacta acgcatcagc agcaagtaat
    22081 tgctcgattt cgcttgtcac tgctaacgca tttaaatcta gaggataaca ttctatcgct
    22141 aattctccga ccgttcgctt agtacctgca acacgccagc ccttattttg taatgcttca
    22201 gctaaaggta gtccgagcca gccaagccct aaaatggtaa tttttttcat catttacgtt
    22261 tctcaagatt aaaattggaa tgaggggaaa ttcaggtaca aaaaaagcac ctcgtggtgc
    22321 tatctatttt atttctagat tggagcggga aacgaggctc gaactcgcga ccccgacctt
    22381 ggcaaggtcg tgctctacca actgagctat tcccgcatat ttgcttaatt atc
//
LOCUS       DANJWC010000020        22424 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.020, whole genome shotgun sequence.
ACCESSION   DANJWC010000020 DANJWC010000000
VERSION     DANJWC010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 22424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22424
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.020"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..189
                     /locus_tag="PWX02_000575"
     CDS             <1..189
                     /locus_tag="PWX02_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HDL1728614.1"
                     /translation="DYRIFVKANPQTLPLFLAENAEQFHYFAKGKVQKMHYWSIPELA
                     IEEADELVKWIRLGLQAV"
     gene            279..992
                     /locus_tag="PWX02_000576"
     CDS             279..992
                     /locus_tag="PWX02_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
                     /protein_id="HDL1728615.1"
                     /translation="MNQKKLVSLIVVIVLAIISQIFSFFDKKEPSKAQEKSTQSSQSH
                     QVSTSSLGNYDVQMKNDRLKQHIVDTDYYTLALSWSPAFCQKQKRNNHGKIPSHLEYQ
                     CGGSADFGWVIHGLWPQSGKARSVDEHPRYCQGDLPMVAEQTIKQYLPESPGATLLQG
                     EWEKHGACAFNQADDYFAKQKELFNQLKLPDTEMSKLALFNWMRANNPHLQNVYLGGS
                     KDELYICYDKAFQPMDCPR"
     gene            992..1486
                     /locus_tag="PWX02_000577"
     CDS             992..1486
                     /locus_tag="PWX02_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HDL1728616.1"
                     /translation="MADLRQLKMQVQRQLKRDLERANHYFNTTFTPPSISYAVRGVKA
                     GVAYLERNEVRFNPVLLQENEQAFIRQVVPHELAHILVYQHFGRVQSHGKEWKMMMET
                     VLGVPAEIYHCFDTQSVQQQFSYQCACQTHQLSIRRHNAVMRDKKSYICRLCKTQLHF
                     VEKS"
     gene            1591..2871
                     /locus_tag="PWX02_000578"
     CDS             1591..2871
                     /locus_tag="PWX02_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL1728617.1"
                     /translation="METIVIVGGGAGGLELATYLGNKLGRKQKAKVVLIDRNATHLWK
                     PLLHEVATGTIDEGTDALSYRAHAKNHSFEFQQGTLVAVDREKKTVSLAPIYNDQNQL
                     LVKERHISYDKLVIAIGSKSNDFGTKGVAENCIFLDGSEQAKDFQKRMMELFLRFSHS
                     ENKDVKIAIVGGGATGIELSAELYHVVKNLNSYGFGKLNRASLKVTLIEAGPRLIPAL
                     PEKVSVSAFHELKKAGVSVRLGTMITEARPDGLMTRLGEKIEADIMVWAAGVKAPEIT
                     REFGFETNRLNQIEIKDTLQTTVDDSVFVIGDCASLIQDGKPIPPRAQAAHQMATRCG
                     KNIVALIDGKEMKPFRFKDKGSLLSFSKFGTVGNLMGNLVSGDMFIEGKIARLAYLSL
                     YRMHQVALHGCVKTGLIVLVGQINRFLRPTMKLH"
     gene            complement(2958..3995)
                     /locus_tag="PWX02_000579"
     CDS             complement(2958..3995)
                     /locus_tag="PWX02_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1728618.1"
                     /translation="MNVLKLISLKSILIASGIFLSAQAAQAEGRLTVYCSVQNNVCEE
                     MTKQFSHKYNVETKFIHGGTGTIFGKVKAEASNPQADIWYGGTIEPHFQAGELGLLEA
                     YRSPKQAEILPQFKSLVESEQGKFTSIVYMLVLGLGVNTEKLAKLGIEAPKKWEDLSD
                     PRLKGEVQLPDPHSSGTTYTFMTTLSTLWGEEKTFDYLKKLNNNVSQYVKSTLVTSNL
                     ARGESAVTVGFVHSYATEKEKGAPIEAIIPEGKVGYALGGVSIIKNARNLDNAKLFMD
                     WVLSKEAQEIPWKKHGVYQTPTNINAEVAPQSTKADALDFVEIDYQKAGSSEEGKRLI
                     DKWLAEIKLAN"
     gene            4217..4913
                     /locus_tag="PWX02_000580"
                     /pseudo
     CDS             4217..4913
                     /locus_tag="PWX02_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249137.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
     gene            4924..5226
                     /locus_tag="PWX02_000581"
     CDS             4924..5226
                     /locus_tag="PWX02_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL1728619.1"
                     /translation="MFAALVLGSQICRFLPEFLPKKVLSSPILQKLNKMLPLVIMLLL
                     LLTSLNFPKNGEGFNLILAQILALIGVLGSYVWLKNTVLSVALGIFGVNVFLSLLG"
     gene            complement(5347..6648)
                     /locus_tag="PWX02_000582"
     CDS             complement(5347..6648)
                     /locus_tag="PWX02_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL1728620.1"
                     /translation="MSLFKFEERGSNARQEIIAGLTTFLAMVYSVIVVPGMLSQAGFP
                     AESVFIATCLVSGLGSILIGLWANAPMAIGCAISLTAFTAFSLVLGQQVSIPVALGAI
                     FLMGVVFTLISATGIRTWILRNLPSSIAHGAGIGIGLFLLIIAATNVKLVVNSGMDIP
                     VKLGDFTSFPVLVSLIGLAAIIGLEKLKVKGSILWVIIAITIVSLIFDPNVKYTGFFK
                     MPSFGENSQFLNLDIMGALNTAILPVVFALVMTAIFDATGTIRAVAGQAGLLDKDGQI
                     INGDKALTSDSFGSILSGLFGTAPAAVYIESAAGTAVGGKTGLTAVVVGIGFLFMLFF
                     QPLAFLVPSYATAPALMYVGLLMLSNVSKLDFSDFVGAMSGLVCAVFIVLTANIVTGI
                     MLGFATLVIGRLISGEANKLNLGTVIIAIILVAFYAFDLAI"
     gene            complement(6781..7755)
                     /gene="secF"
                     /locus_tag="PWX02_000583"
     CDS             complement(6781..7755)
                     /gene="secF"
                     /locus_tag="PWX02_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HDL1728621.1"
                     /translation="MAIENVQQDVAEIKLPYKLIPFMKYRYVGFIFSLIVTALCIFTI
                     ATKGFNWGLDFTGGTVIETQFSQPADLAKLRTELENSGYGNALVQTTGSQKDLMIRLA
                     ASAGNMSVGNKVMEMIHQKLDPNATIKSIEFVGPNVGEELTQGAIYATLATLAMLLIY
                     VGTRFEWRLAVGGIVALFHDVLVTIGIFSFLQIEIDLTFVAAILSVVGYSLNDSIVVF
                     DRVRENFSKVRRSSSIEIIDISLSQTLSRTLMTSVTTLFVVLALFYLGGPTLHSFSLA
                     LLIGIGFGTYSSIYIAIGVALQLGLKREHMVQPKVEKEGADQDAFIDY"
     gene            complement(7765..9606)
                     /gene="secD"
                     /locus_tag="PWX02_000584"
     CDS             complement(7765..9606)
                     /gene="secD"
                     /locus_tag="PWX02_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HDL1728622.1"
                     /translation="MLNRFPLWKNLMVIFVIAIGALYALPNLYGDDPSVQISGTRGQQ
                     ATPETLSQVQSTLSAMNIQPKSAVLENGSILVRLESSEQQLPAKEKISEALGNNYSVA
                     LNLAPATPQWLSDIGGSPMKRGLDLLGGVRFLMEVDMNTALAKQQDTLQDSLRSDFRK
                     EKLQYQAVKKGENFATEVEFADNDTAEKAVRLVRLSHPTLDILVNDNKVTLTPSAQAL
                     SESRTMAIEQNLSILRKRVEELGVSEPTIQRQGADRIVVELPGVQDTARAKELLGATA
                     TLEFRLVNTEANVASAAKGIVPADSELQNTRDGEPVVLRRKPSLGGEHIIDATSGTDE
                     RGLPQVSIRLDTEGGEMMGEITKMAIQKPMATLYTEFKDSGRKDANGKVILEKHQEVI
                     NVATIQTRLGNQFQITGINSPAEAQNLAVLLRSGALIAPIVIVEERTIGASLGADNVK
                     QGMEASILGLGLTILFCLAFYKVFGIFASLALIVNMILTIGLMSLIGATLTMPGIAGI
                     VLAVGMSVDANVLIYERIKEEIRNGRPIQQAIHEGYNGAFSSIFDSNLTTILTSLVLY
                     GVGTGPVKGFAVTLALGVIISMFTAITGTRMLVNWVYGGKRVKKLWI"
     gene            complement(9646..9939)
                     /gene="yajC"
                     /locus_tag="PWX02_000585"
     CDS             complement(9646..9939)
                     /gene="yajC"
                     /locus_tag="PWX02_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDL1728623.1"
                     /translation="MQQGGGMEMIFILIIFGAIFYFMIYRPQAKRQKQQRDLLASLTK
                     GDEVLTSGGLIGKITKVTADNDNIVIALNDTTEVTIKRDFVVAVLPKGSLKSL"
     gene            10140..10697
                     /locus_tag="PWX02_000586"
     CDS             10140..10697
                     /locus_tag="PWX02_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDL1728624.1"
                     /translation="MKKLFAQAVIFGAFFASSAFAHNVQLNQALPAVSVAKDGELAVA
                     GGKVSYKNWHSSSLAGKVRVIHHFAGRSSVKEKNEDLMTAIKNAGFDRSKYQTVTIVN
                     ADDAIVGTGVFVKNSVENGKLDNPHSQVVLDQQGSVKKAWGLKEKESFIAVLDKSGKV
                     QFTSEGKLSAKQIQEVIGLVKTLTQ"
     gene            complement(10738..11706)
                     /locus_tag="PWX02_000587"
     CDS             complement(10738..11706)
                     /locus_tag="PWX02_000587"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HDL1728625.1"
                     /translation="MKLFTLENAFLRITLSDVGASWLSCIVKMPNEEREVLVTTSAKN
                     WQKQTAFFGATIGRYANRIANAEYRLNGKTYQLAKNNGEHNLHGGELGADKIVWQLES
                     QSEQAVKFSYFFADGEEGFGGEVKAFVEYRLTENELQISFDAVANQDTPLCLTNHAYF
                     NLQGSETILDHELIINADGYLPVDASGIPNAPLKKVESTSFDFRNAKKIGQDLLADDD
                     QKAVKGYDHSFHLAKNSENPTACLAVEDLKMELRTSMPALQLYTGNWLKGQPNLTKGE
                     YEDYAGVALEPEFFPNSLNQPELLQFGGITKAGEEYHHTISYRFII"
     gene            complement(11832..12995)
                     /gene="galK"
                     /locus_tag="PWX02_000588"
     CDS             complement(11832..12995)
                     /gene="galK"
                     /locus_tag="PWX02_000588"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDL1728626.1"
                     /translation="MKPQEIALQQFEQHFGYKAERTVFAPGRVNIIGEHTDYNDGFVM
                     PCAINYGTAVSFAKRSDNKWRVFAIDLNQQDEFDLSQAIEPSEQKWANYVRGVVKFIQ
                     AKHPEFKQGADLAITSDVPMSSGLSSSAALEVSIGKTAQLLGDLPLSLPQIALIGQEA
                     ENKFVGCNCGNMDQLTSALGQQDHLVMIDCRSLEITPTPVPQGYSIAIINSNVKHDLV
                     TGEYNSRREQCEFAAHFFGKAKLRDVTAEQFIERASELSEENELAYKRAKHIISENQR
                     VLEAVEALKANDMVKLGELMAQSHDSMRDDFEITCPEVDCLAEIAQQAIGKNGGARMT
                     GGGFGGCVVCLVPNDKVEDLRQAVANNYEKQTGIKETFHVCTASDGVRECPAR"
     gene            complement(13005..14051)
                     /gene="galT"
                     /locus_tag="PWX02_000589"
     CDS             complement(13005..14051)
                     /gene="galT"
                     /locus_tag="PWX02_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDL1728627.1"
                     /translation="MSEFVLNDHPHRRFNPLKNQWILVSPHRAKRPWQGQQEEIVQDE
                     KPSYDPSCYLCPGNKRITGEQNPNYTKPFVFKNDFSALLPDTPAPIPNDDPLFQISHT
                     QGESRVICFSPDHSKTLPQLSIEEITDVVTVWQEQANELKEHYQWVQIFENKGSMMGC
                     SNPHPHGQIWASNFLPNEIAIEDECQAKYFAQYGSPLLLDYAQRELDKQERIVAETED
                     WIAVVPYWAAWPFETLLLPKAKQFKSITDLNEAEKADLALALKKLTTRYDNLFNISFP
                     YSMGFHFAPFNGKENAHWQLHAHFYPPLLRSATVRKFMVGYEMMAESQRDLTPEQAAE
                     RLNAVSDTKHYKDK"
     gene            14264..15115
                     /locus_tag="PWX02_000590"
     CDS             14264..15115
                     /locus_tag="PWX02_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF817 domain-containing protein"
                     /protein_id="HDL1728628.1"
                     /translation="MERLNKFDAWLMNHKPNARLSGASRLLTEFWFFGLKEARACLFA
                     GLFFIAMFSVPKTGLWGLPRYDILLIFAIVVQIAMVKLGLETWDELKAISLFHLVGFV
                     LEVFKTSSNIQSWAYPDFAYTKLFNVPLFTGFMYAAVGSYIIQAWRLFDLKIKSHLPY
                     WLATLTALMIYLNFFTHHYIGDYRWYIAAFALGLYARTFVFFTPYDITRKMPLLLSFF
                     LIGFFIWLAENIGTFAGIWRYPNQFGAWSMVHMSKWSAWALLVMMTFTIIVHLKHIKQ
                     TVRVSSD"
     gene            complement(15201..16286)
                     /locus_tag="PWX02_000591"
     CDS             complement(15201..16286)
                     /locus_tag="PWX02_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL1728629.1"
                     /translation="MEYRYYITTALVIAVLQLFLFNFAKTVSWLFNLSSKNRKTVGIL
                     TFLVANGLVILHLTRTFALFRIMAFILVLLLFAFFISFFTACIYKIFNGNTTVNRWLK
                     IAYPFAFFGLLGLGYYNAYTPTVVHYSVKLDKPLEKPLRIGMASDLHLGKLFGTKQLD
                     QLAEIFNQQKVDLILLPGDIMDDNTEVYVADKMQPHLAKLKAPLGVYATLGNHDFFGA
                     QETIAKEITDAGITVLWDQTAEINGKFTLIGRNDDLVKNRPSAEQLLQNVNTKLPVIL
                     LDHRPTEIEQHAKLPIDIQVSGHAHKGQVFPASIITKLIYRLHYGYEKIGFGHYFVTS
                     GYGFWGVPMRLGSQSEVMIIDVEGENP"
     gene            complement(16308..17288)
                     /locus_tag="PWX02_000592"
     CDS             complement(16308..17288)
                     /locus_tag="PWX02_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDL1728630.1"
                     /translation="MLFDSIQFSIGVTLPTILLMLFGIFPRRRKFIDDTFCNMASKVV
                     FNFTLPPMIFLNMLKSPLDFSSQINLISTGVGGSILIYLWAEWWAARHIENRRYRCIF
                     TQGTFRTNAAILGIALILNAYGDKALAAASVYISCLVITFNVLAVITLTSSLSEKKVN
                     FGRLILSILRNPLILAVIAGLTFNYFELSHQIPTPLESAGKLMASLTLPLALICTGAS
                     INFKQLKQFNQGVESTINKIVLFSASIRLIFAPIFLLLLGKFVFQLPPMELGIVFVAA
                     SAPVASATYAMTRNYGGDGEAAANLIGITTLGSMFSASIGLFLLRQIGWV"
     gene            complement(17391..18489)
                     /gene="prfB"
                     /locus_tag="PWX02_000593"
     CDS             complement(join(17391..18413,18415..18489))
                     /gene="prfB"
                     /locus_tag="PWX02_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014550889.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDL1728631.1"
                     /translation="MFELNPIKNQLTDLSERTAVIRGYLDFEHKVERLEEVNAELEQP
                     DVWNKPEKAQALGKERAMLEQIVNTIKALEQGIEDVEGLIELAVEAEDQDTFNEAQVE
                     ADELEKKLADLEFKRMFSGPHDSADCYLDLQAGSGGTEAQDWTEMLLRMYLRWAESKG
                     FKAELMEASDGDVAGIKSATVKISGDYAYGWFRTETGIHRLVRKSPFDANNGRHTSFS
                     AAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNKTESAVRITHIPSGIVVQCQN
                     GRSQHQNKDQAMKQLKAKLYELEMSKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DHRTGVEHRTTQQVLDGDLDKFVEASLKAGL"
     gene            18745..19707
                     /locus_tag="PWX02_000594"
     CDS             18745..19707
                     /locus_tag="PWX02_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005823743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDL1728632.1"
                     /translation="MAKRPSDIDATLLQLEILRRIPKLPAKIDAKSLHQQLLDAGFER
                     DIRTVQRSLKMLCEHFDIECDERSKPFGYSWKIRSEGLALPILNEQQSLLLKLAEQQL
                     KYLLPANLMASMKPFFEQADRMVSGAKDKPEYQWLNKVCSTPTSLPLIPAKIKEEIFN
                     SVSQALFQNKLLKIEYQNQHGKKHQATIMPLAISQQGASIYLVCRYEGFDDNRLLALH
                     RIRKAEISTFSFERPKDFNLQAYQDAGHLGFGNGEKASLTFSISRDAGFHLTETPLAK
                     DQKILEESETHYRIQAIVADNDMLTWWLRKFGEDIWDIEKEALK"
     gene            19954..20595
                     /locus_tag="PWX02_000595"
     CDS             19954..20595
                     /locus_tag="PWX02_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HDL1728633.1"
                     /translation="MPFQLTFCQQAGNQHQKNQDALFNGKAVYQWQLKNAESYLLDND
                     KVIFGVADGVSSHPRNHLASHYLMALLSQCQSLDSQWLRQAHGDFCEKLAKDYFGASC
                     TFVACELHHNGRGRMVNVGDSRAYKITANGEWQQLSFDHTILAEMKQKGLVKEGSNYA
                     SMYNGLTDCIVADFDADDFRIHIAEFQLEKGESILLCSDGLHEQLAPEAIQAI"
     gene            20762..21583
                     /locus_tag="PWX02_000596"
     CDS             20762..21583
                     /locus_tag="PWX02_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HDL1728634.1"
                     /translation="MKNDFHYAAELIRNADGILITAGAGMSVDSGLPDFRSVGGLWNS
                     YPPLKKFNLQFMQIATPLAYQERPELAKWFYAHRLNQYRQAEPHQGYAILKRWAESKQ
                     HGYFVFTSNVDGHFRKAGFSEDRIYECHGTLDHLQCLANCKNKSWWAYDYQPVTDDEH
                     CLVLSEPPTCPDCGGLARQNVLMFNDWSYAEGYQLTKETLLDEWLRKAQRPVVIEIGA
                     GKVVPTVRNFSERTAKQKKGGLIRINPIDAGVPKMHFLSLEMGGLEALQGIEECL"
     gene            21659..>22424
                     /locus_tag="PWX02_000597"
     CDS             21659..>22424
                     /locus_tag="PWX02_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDL1728635.1"
                     /translation="MSQNNTEKEIAEIRKIEDVVRTKLSIPDYQRPYKWSVKHITQLL
                     NDLYFHFENGKKVYRVGSVVIHTYTGKDENSESKTKFDIVDGQQRLISLSLILFLLSN
                     DNTFPLLSEKLSHSISADNLIKNKVAVEQFIRDRVQDKQEFATYILETCEFVYIELGD
                     IDEAFQFFDAQNARGKSLAPYDLLKAYHLRELKADKETIYQCVENWEQAVEAEIADLK
                     EIISETLFRLRRWQKYQTAEKFTNKELDTFKGASSDK"
BASE COUNT         6520 a         4565 c         4802 g         6537 t
ORIGIN      
        1 gattatcgca tttttgtcaa agctaatccg caaaccttgc cgttattctt agctgaaaat
       61 gccgaacaat ttcactattt tgctaaagga aaagtgcaaa aaatgcacta ctggagcatt
      121 ccggaattag cgattgaaga agccgatgaa ctggtcaaat ggattcggct aggcttacaa
      181 gcggtgtaat ttagcgaaaa ttttgtaaaa aaacgctaaa atatgcccgc ttgttagggg
      241 ggggacctaa tatttctata ctttaagatt ttatatttat gaaccaaaaa aaattagttt
      301 ctttgattgt cgttattgtg ttggcaatca tttctcaaat ttttagcttt tttgataaaa
      361 aagagccttc aaaagcacaa gaaaaatcga cccagagtag tcagtctcat caagtttcaa
      421 cttcatcact agggaactat gatgtgcaga tgaaaaacga ccgtttgaaa cagcatattg
      481 tagataccga ttattacacc ttagcgttgt cgtggtcgcc ggcgttttgc caaaaacaaa
      541 agcgaaataa tcacggtaaa attccgtctc atttggaata tcaatgtggt ggctcagcgg
      601 attttggttg ggttattcac ggcttatggc cgcaaagtgg caaggcaaga agtgttgatg
      661 aacaccctcg ttattgccaa ggcgatttgc cgatggtggc agagcagacg attaagcaat
      721 atttacctga atcgccgggg gcaactttgt tgcaaggcga atgggaaaaa cacggtgctt
      781 gtgcctttaa ccaagcggat gattacttcg ccaaacaaaa agagctattt aatcagctca
      841 aattaccgga taccgaaatg tctaaattag cactgtttaa ctggatgcga gccaataacc
      901 ctcatttgca aaatgtctat ttagggggta gcaaagatga gctgtatatt tgctatgata
      961 aggcatttca accaatggat tgcccacgct aatggcagat cttcgccaac tgaaaatgca
     1021 ggttcagcgc caattaaagc gtgatcttga acgtgcaaat cactatttta ataccacttt
     1081 tacgccacca agtattagct atgcggtgcg tggcgtaaaa gccggtgtgg cttatcttga
     1141 acgcaacgaa gtacggttta atcctgtgtt attgcaagaa aatgaacagg catttatccg
     1201 ccaagttgtg ccgcacgaac tggctcatat cttggtttat caacattttg ggcgggtaca
     1261 atctcatggt aaagagtgga aaatgatgat ggaaaccgta ctgggtgtgc cggcggaaat
     1321 ttaccattgt tttgataccc aaagtgtgca acagcaattt agttaccaat gtgcctgcca
     1381 gactcaccag ctttcgattc gccgacacaa tgccgtgatg cgagataaaa aaagctatat
     1441 ttgccgttta tgtaaaactc aactccattt tgtagaaaaa agttaatgtg gagaaaagtt
     1501 aagagaatgt tacgatttag ctagacattc ctgtttaaac gcctacaatt tgccgtagtt
     1561 tttattttta attttatttc cggagtattt atggaaacaa ttgtaattgt aggtggcggt
     1621 gcaggcggtt tggagcttgc aacctatcta ggcaacaaat tagggcgtaa acaaaaggca
     1681 aaagtggtgc taattgaccg caatgcaacg catttatgga aaccactgtt gcacgaggtg
     1741 gcaaccggta ctattgatga gggaactgat gcgttaagct atcgggctca cgctaaaaat
     1801 catagttttg aatttcagca aggtacgctg gtggcggtag atagagagaa gaaaacggtt
     1861 tcactcgccc cgatttataa tgatcaaaac caacttctcg ttaaagaacg ccatatcagt
     1921 tatgacaaat tagtgatcgc gatcggcagt aaatccaatg attttggcac caaaggggtt
     1981 gcagaaaatt gcattttctt agatggctca gagcaagcaa aagatttcca aaaacgaatg
     2041 atggagctgt ttttacgttt ttcacatagt gaaaataaag atgtaaaaat tgcgattgtg
     2101 ggggggggag caacaggtat tgagctttca gcggagcttt accacgtggt gaaaaattta
     2161 aattcttacg gctttggtaa attaaaccgt gccagcttga aagtcacttt aattgaggca
     2221 ggccctcgtt tgattccggc attaccggag aaagtgtcgg tttctgcttt ccacgaactg
     2281 aaaaaagccg gtgtttcggt gcgtttaggc acgatgatta ccgaggctcg cccagacgga
     2341 ttaatgaccc gcttaggtga gaaaattgag gcggatatta tggtttgggc tgccggagta
     2401 aaagcacccg aaatcacccg tgaatttggt tttgaaacta accgcttgaa ccaaattgag
     2461 attaaagata cgttacaaac caccgttgat gattcggtat ttgtcattgg tgattgtgca
     2521 tcgttaatcc aagatggcaa accaattcca ccaagagcgc aagcggctca ccaaatggca
     2581 acccgttgtg gtaaaaatat tgtggcgctg attgatggga aggaaatgaa accgtttcga
     2641 tttaaagata aaggctcgtt gctttcattc tcaaaattcg gcacggtggg caacctaatg
     2701 gggaatttag tgagcggtga tatgtttatt gagggtaaaa ttgcgcgatt ggcgtatctc
     2761 tcgctttatc gaatgcacca agtggcatta cacggctgtg tcaaaaccgg cttgattgtg
     2821 ttggtagggc aaattaaccg tttcttaaga ccaacaatga aactacatta aacaaaaatc
     2881 cgcatcaaat gatgcggatt ttttatggca aaataacaag cggttatttt cttgcctatt
     2941 tttgcaaata ctcacgccta gtttgctaat ttaatttccg ccaaccattt atcaattaag
     3001 cgtttgccct cttcgctgga acctgctttt tggtaatcaa tttccacaaa gtctaacgca
     3061 tcggctttgg ttgattgtgg agcaacttcg gcgttgatgt ttgttggggt ttggtaaaca
     3121 ccgtgttttt tccaaggaat ttcctgtgct tcttttgaaa gcacccaatc cataaagagt
     3181 ttggcgttat ctaaattacg ggcatttttg ataatgctga caccgcctaa cgcatagccc
     3241 actttacctt ccggaataat ggcttcaata ggcgcacctt tttctttttc agtggcataa
     3301 ctatgtacaa agcctaccgt aaccgcactt tcaccacgag caagattgct ggtgactagg
     3361 gtgcttttca catattgcga gacgttattg ttgagttttt tcagataatc aaaggttttc
     3421 tcttcgcccc ataaggttga aagagtggtc ataaacgtat aagtcgtgcc ggagctatga
     3481 ggatcaggca gttgtacttc accttttaag cgaggatctg ataaatcttc ccattttttc
     3541 ggtgcctcaa tgcctagttt ggcgagtttt tcggtattca cgcctaaccc taatacaagc
     3601 atataaacga tagaggtaaa tttaccttgt tcactttcaa ctaaggattt aaattgtggc
     3661 agaatttcag cttgttttgg tgaacggtag gcttccagta agcctaattc gcctgcttga
     3721 aagtgaggtt caatcgtgcc gccataccaa atatctgcct gagggttcga tgcttccgct
     3781 tttacttttc cgaaaattgt accggttccg ccgtgaatga atttagtttc gacattatat
     3841 ttgtgtgaaa attgcttggt catttcttca caaacattat tttgtaccga gcaataaaca
     3901 gttaaacgac cttctgcttg tgctgcttga gctgataaga aaatgccaga ggcgattaag
     3961 atcgatttta atgagattaa tttgagtacg ttcatcactt tcctgaaaac ataaaataaa
     4021 aggtagtgaa gtctagcatt tcaaagcgct ggttgaaaat agttaatcgc aatgaagtag
     4081 ggcaattgtg cctattactt taatatttac caactatttg caagttttag caaaaaataa
     4141 ccgcttgtag tagctcttgc tattttgtca aaattcagaa taataaatgc ctttagtcta
     4201 atgttaaatt ttagttatgg aaaatagttg gaaatatgct tttaaattaa ccacgccgat
     4261 ttttatgggc tattttgcgg caggggttgc ttacggaatg cttgccacca atgccggaat
     4321 gcctgcgtgg tttacgattg taatgagttt taccgtggta tcggggacgg cacaatatgc
     4381 agcgattcca ttttttgtct ccggtgttgg ggcggtgtcg gtctttctca gtacgctttt
     4441 aatgagccta cgttttagtt tttacacctt aaatatgatg gagcataagc cgatatccaa
     4501 agcgaaaagt ctggtcgcta ttgcaggttt aactgatgaa ggctttgcgg ttatcttcat
     4561 taccgaaaga gcaaaagcaa gcatcattct ttaaaatcgc tgtgctatgt gtggcttatt
     4621 ggacattttc cacgattgtc ggtgtgttgt taggcgattc agtggcgaac tatattccgc
     4681 atttggattt tgctctgcct tgtttgtttg caattttagc ctatgagcaa tacaaaaacc
     4741 aaaaacaatg gaaaccgatt gtgattgcgt taatcggatt tttacttgct cgtcagatta
     4801 ccgaaaccag cgtgttattg gtggcaattt tagtagcgat tgtcattgtg gctttattac
     4861 ctcaatcttc ttcaatcaaa ctagagaaag gagtagaaaa tgagcacaaa tgatattttg
     4921 ataatgtttg cagcattagt gctcggctcg caaatttgtc gttttttgcc ggaattttta
     4981 ccgaaaaagg tgttatcttc gcccattttg caaaaattaa acaaaatgtt accgcttgtg
     5041 attatgcttt tattgctttt aacgagccta aactttccta aaaacgggga aggctttaac
     5101 ttaatattgg cacaaattct ggctttaata ggcgtattag gtagctatgt ttggcttaaa
     5161 aataccgttt taagcgtagc gttggggatt ttcggtgtga atgtattttt atccctgtta
     5221 ggataaatac caaaggtcat tttataggag cgaaacacct tcgctcaatc attaacagat
     5281 gtggggcgaa aaatttttcg ccccttattt atcacaagcg gtctttttct ttcaattttt
     5341 tgcaaattaa atcgctaaat caaacgcata gaaggcaact aaaataatcg caatgattac
     5401 tgtacctagg tttaatttat tggcttcgcc tgaaattaag cgaccaatca caagggtggc
     5461 gaaacctagc ataatgcctg tcacaatgtt ggcggttaat acgatgaaca ccgcacagac
     5521 taaacctgac attgcaccta caaaatcgct gaaatctaat ttagagacat tacttagcat
     5581 taataagccc acatacatta atgccggtgc ggtggcatag cttggtacta agaatgcaag
     5641 aggttggaag aacagcataa ataagaaacc gataccaacc actacagcgg ttaaacctgt
     5701 tttaccgcca acggctgtac ctgctgccga ttcaatatac actgccgctg gtgctgtacc
     5761 aaataagccc gataaaattg agccgaatga gtcagaagtt agggctttat cgccattgat
     5821 aatttgtccg tctttatcta ataaaccggc ttgccctgca accgcacgga tagtacctgt
     5881 tgcatcaaaa atagcggtca ttactaacgc aaacactacc ggtaaaatgg cggtatttaa
     5941 tgctcccata atatcaagat ttaggaattg cgaattttca ccaaaactcg gcattttgaa
     6001 aaagcctgtg tattttacgt tagggtcaaa aattaaactt acgatggtaa tagcaataat
     6061 gacccataaa atactgcctt ttactttcag cttttctaac ccgataatgg cggctaaacc
     6121 gattaatgac actaaaaccg gaaatgaagt gaagtcccct aatttaaccg gaatatccat
     6181 accgctattg accactaatt taacgttagt ggcggcaata atcagtaaga ataaaccaat
     6241 accaattcca gcaccgtggg caatgctgct tggcaaatta cgtaaaatcc aagtacgaat
     6301 accggtagca gaaatcaagg taaatactac gcccattaag aaaatcgccc cgagtgcaac
     6361 aggaatagaa acttgttgcc ctaatactaa gctaaaggca gtaaaagcag taagtgaaat
     6421 cgcacaaccg attgccattg gggcatttgc ccataaaccg attaagatag agccgagacc
     6481 tgaaactaaa caggtggcaa taaataccga ttcggcagga aagcctgctt ggcttagcat
     6541 tcccggcaca acgatgacgg aataaaccat ggctaaaaag gtagttaaac cggcgatgat
     6601 ttcttgacga gcgtttgaac cacgctcctc aaatttaaat aacgacataa gaacctcaaa
     6661 agaagaaaat gaaaggggaa attttaggat gcggaatttt acagaaattt aaaataatta
     6721 gcaaacgttt gcgtaagaag tgaaagaaaa gcaggaataa atcctgcttt tgtttgatgg
     6781 ttagtaatcg atgaaagcgt cttgatctgc cccttccttt tcaactttag gctgaaccat
     6841 atgttcacgt tttagcccaa gttgtagtgc cacaccaatt gcaatgtaga tggatgaata
     6901 agtaccgaaa ccgataccga tcaataatgc aagtgagaaa ctatgaagcg ttggaccacc
     6961 tagatagaat aaggctagta ccacaaagag ggtggttact gatgtcatta aagtacgcga
     7021 aagtgtttgg ctcaaggaaa tatcgataat ttcaattgag ctggagcgac gaactttaga
     7081 aaagttttca cgtacacggt caaacaccac aattgagtca tttaacgagt aaccgacaac
     7141 agataaaatc gctgctacaa aggtcaagtc aatttcaatc tgtaagaaag agaagattcc
     7201 gatcgtcact aatacatcgt gaaataaagc cacaataccg cctaccgcta aacgccactc
     7261 aaaacgtgtt cccacgtaaa taagtagcat agcaagtgtt gccaaagtgg cataaatagc
     7321 cccttgggtt agctcttccc ccacgtttgg gccaacgaac tcaatgctct tgatagtcgc
     7381 attcggatct aatttttgat ggatcatctc catcacttta ttcccaacag acatattacc
     7441 ggcactggcg gcaagtcgga tcattaaatc tttttgactg cctgtggttt gcactaaggc
     7501 attgccgtag ccgctatttt caagctccgt acgcagtttt gctaaatcag caggttgtga
     7561 aaattgagtt tcgattactg tcccgccggt aaaatctaac ccccagttaa agccttttgt
     7621 cgcaatagta aaaatgcata aagcagttac aatcagcgaa aaaatgaatc cgacatagcg
     7681 gtatttcata aaggggatta atttataagg cagtttaatt tctgctacat cttgttgtac
     7741 attttcaata gccatttttc caccttaaat ccaaagtttc ttcacgcgtt tgccaccgta
     7801 aacccagttt acaagcattc gagtacctgt aattgcggta aacatagaaa taattacccc
     7861 taatgcaagc gttaccgcaa agccttttac cggacctgta cctacaccat aaagcacaag
     7921 agaagttaaa attgtggtga ggttagagtc aaaaatactt gagaatgcgc cattataacc
     7981 ttcgtggatt gcttgttgaa ttgggcgacc gttacgaatc tcttctttga tccgctcgta
     8041 gattagtacg ttggcatcaa ctgacatacc cacagcaagt acgataccgg caattcccgg
     8101 catagtaagg gttgcaccga ttaatgacat taaaccaatc gttaagatca tattcacaat
     8161 caaggcaaga gaggcaaata taccaaatac tttataaaaa gctagacaga acaggatcgt
     8221 taagcctaag cctaaaatac tagcttccat accttgttta acattatctg ccccaagaga
     8281 agcaccgata gtacgctctt ccacaatcac gattggagca atcaacgcac ctgaacgtaa
     8341 taataccgca aggttttgag cttccgctgg tgaattaatc cctgtaatct ggaattgatt
     8401 acctaaacgg gtttgaatgg ttgcaacgtt aattacctct tggtgttttt ctaaaatgac
     8461 tttaccgtta gcatctttac gacctgaatc tttaaattcg gtataaagtg ttgccatcgg
     8521 tttttggatt gccattttag taatttcacc catcatctcg ccgccttcgg tatcgagtcg
     8581 gatactcact tgcggcaagc cacgctcatc agtgcctgag gttgcatcaa taatatgttc
     8641 accccctaac gaaggtttac ggcgtaatac aactggctcg ccatcacggg tgttttgcag
     8701 ttctgaatca gccggcacaa tgcctttcgc cgcagaggcc acattggctt cggtattgac
     8761 taaccggaac tcaagggttg cagttgcccc taaaagctct ttggcacgag cggtatcttg
     8821 cacacccggt aattcaacta cgatacgatc tgcaccttga cgctgaatgg tcggctcaga
     8881 aacgcccaat tcctcaacac gtttacgcaa aatagaaagg ttttgttcga ttgccatcgt
     8941 gcgagattca gaaagagctt gtgctgatgg cgttaaggtg actttattat catttaccaa
     9001 aatatcaagc gttgggtgag agagacgcac taaacgcact gctttttcgg cggtatcgtt
     9061 atcggcaaat tcaacctcag ttgcaaaatt ttcgcctttt ttgaccgctt gatattgcaa
     9121 tttttcttta cggaaatcac tacgcaggct gtcttgcaaa gtgtcttgtt gttttgcaag
     9181 cgccgtgttc atatccactt ccattaagaa acgaacaccg ccaagcaagt ccaagcctcg
     9241 cttcatcggg ctaccgccaa tgtcagacaa ccactgtggc gttgctggtg caaggtttaa
     9301 ggcaacagag tagttattcc ccaatgcttc tgagattttt tctttggcag gcaattgctg
     9361 ttcgctgctt tcgagacgaa ccaaaatcga gccattttct aacacggcag atttcggctg
     9421 aatattcatt gctgaaagtg tggattgcac ttgagacagt gtctcaggtg tggcttgttg
     9481 cccacgagta ccggaaattt gtaccgaagg atcatcgcca tataaatttg gaagggcata
     9541 taaagcacca atggcgatca cgaagatcac cattaaattc ttccatagag ggaaacggtt
     9601 taacacaata actccccgaa tgttaaagtt ggataagagg gttgattaaa gtgattttaa
     9661 tgaacctttt ggtaacaccg caacaacgaa atcacgtttg atggtcactt cagttgtgtc
     9721 atttaatgca atcacgatgt tatcgttatc agcggtcact ttggtaattt taccgattaa
     9781 accaccgctg gttaatactt catcgccttt tgtcaagctt gccagtaaat cacgttgttg
     9841 tttttggcgt tttgcctgag gacgataaat cataaaatag aaaattgcac cgaaaataat
     9901 caggataaag atcatttcca ttccgccacc ttgttgcata aaaactcctt aaatgtttga
     9961 gtggtataaa aataagtgtt ataaaaaacg cctgttaaaa taccataaat tcaatgaatt
    10021 aacgagtaga aaaattcatc gaacgctttt cttttaaaca aaaatttgag tagaatcagc
    10081 acttttagtt taggattagg ttcttggcgt gttcttgcgc tgacacaata ggagaaaaaa
    10141 tgaaaaaatt attcgctcaa gcggttattt ttggggcttt ttttgcaagt tccgcttttg
    10201 ctcacaatgt gcaattaaat caagcattac ctgctgtttc ggttgcaaaa gacggggaat
    10261 tggcagtggc aggtggaaag gttagctata aaaattggca ttcatcatct ctggcaggta
    10321 aagtccgtgt tattcatcat tttgccggta gaagttcggt gaaagaaaaa aatgaagatt
    10381 tgatgacagc aattaaaaat gccggttttg atcgctcgaa ataccaaacg gtgacgattg
    10441 taaatgctga tgatgcgatt gtcggcacag gggtatttgt gaaaaatagt gtggaaaacg
    10501 gcaaacttga caatcctcac agccaagtag tgttagacca gcaaggcagt gtgaaaaaag
    10561 cgtggggctt aaaagagaaa gagagcttta ttgcagtgct ggataaatcg ggtaaggtac
    10621 aatttacctc tgagggcaaa ctctctgcaa aacagattca agaagtgatt gggttggtga
    10681 aaacattaac gcaataacca gtgtttttat gggcggattt tgtatccgcc catcattcta
    10741 gataataaat cgataactta tcgtgtgatg atactcttct cctgcttttg ttatcccgcc
    10801 aaattggagt aattcaggct ggtttagtga atttgggaaa aactcaggct ctaatgctac
    10861 accagcgtaa tcttcatatt cgcctttagt caaattaggt tgtcctttta gccagttgcc
    10921 ggtgtaaagt tgtaatgctg gcatggaggt gcggagttcc atttttaagt cttccacggc
    10981 taaacaagcg gtcggatttt cactattttt tgcaagatga aaggagtgat cgtaaccttt
    11041 taccgctttt tggtcgtcat cagccaataa atcttgccca atttttttag cattgcggaa
    11101 gtcaaaactg gtactctcca cttttttcag tggagcatta ggaataccgc tggcatcaac
    11161 cggtaagtat ccgtctgcat taattatcag ctcgtgatct aaaatcgtct ctgaaccttg
    11221 caaattaaaa taggcgtgat tagttaagca aagcggtgta tcttggttag caacggcatc
    11281 aaagctgatt tgaagctcat tctctgttaa gcgatactcc acaaaagctt tcacttcgcc
    11341 gccaaatcct tcctcgccat ctgcaaaaaa ataactaaat ttgaccgctt gttcgctttg
    11401 gctttcaagt tgccacacta ttttatctgc cccaagctcg ccaccgtgga gattatgttc
    11461 accgttattt ttagcgagct ggtaggtttt gccgtttaag cgatattcgg cattggcaat
    11521 gcggttagcg taacgcccaa tggtcgctcc aaagaaagcg gtctgttttt gccaattttt
    11581 tgcagacgtg gtgactaata cttcccgttc ctcgttcggc attttgacaa tgcacgaaag
    11641 ccaagaagcc ccaacatcag aaagggtaat gcgtaaaaac gcattttcaa gggtgaataa
    11701 tttcatcggt tgaccttaat tacctagcac ggcatagccg tactaggtta agcataattc
    11761 agccctgcta gggctgaggt tatttatttt agagtagcgg agctacttcc ttatgcttaa
    11821 cccggtacgc tttagcgtgc cgggcattct cgcaccccat cggaggcagt acaaacgtgg
    11881 aaggtttctt taatgccggt ttgcttttcg taattgttag caacggcttg gcggagatct
    11941 tccactttgt catttggcac taggcacacg acacaaccgc caaagccgcc gccagtcatt
    12001 ctggctccgc cgtttttacc gatagcttgt tgggcgattt cggctaagca atccacttca
    12061 gggcaggtga tttcaaaatc atcacgcatt gagtcgtgag attgagccat caactccccg
    12121 agtttgacca tatcattcgc ttttaaggcc tctactgctt ccagaactcg ttgattttcg
    12181 ctgatgatat gttttgctcg tttatacgcc agctcattct cttcactaag ttctgaggct
    12241 cgttcaataa attgttccgc tgtgacatca cgcaattttg ctttgccgaa gaagtgggcg
    12301 gcaaattcgc actgttcacg gcgactgttg tattcgccgg taaccaagtc gtgttttacg
    12361 ttggagttaa taatcgcaat cgaatagcct tgtggcacag gggttggggt gatttctaaa
    12421 ctacggcagt cgatcatcac taaatgatct tgttgcccga gagcggaggt gagctgatcc
    12481 atattgccgc agttgcagcc gacaaattta ttctcagcct cttgcccgat taatgcgatt
    12541 tgcggtaaag aaagcggtaa atcgccgagt aattgagcgg ttttaccaat cgacacttcc
    12601 aatgcggcgg aagaacttaa acctgatgac atcggcacat cgctggtaat ggctaaatct
    12661 gctccttgtt tgaattcggg gtgtttggct tgaataaatt tcactacgcc acgcacataa
    12721 ttcgcccatt tttgctcgct tggctcaatc gcctggctta aatcaaattc atcttgttgg
    12781 ttgagatcaa tggcgaacac ccgccatttg ttgtcactac gtttggcaaa gctaaccgct
    12841 gtgccgtaat tgatggcaca aggcattaca aacccatcgt tgtaatcagt atgttcgccg
    12901 ataatattca ctctgcccgg ggcaaatacc gtgcgttctg ctttataacc aaaatgttgt
    12961 tcaaattgtt gcaaagcgat ttcttgtggt ttcatttcaa ttccttattt atctttatag
    13021 tgtttggtat cacttactgc atttaaacgt tcggcagctt gttccggggt taaatcacgt
    13081 tggctttctg ccatcatttc ataaccgacc ataaatttac gcactgttgc cgaacgcaag
    13141 agcggcggat agaagtgagc gtgtaattgc cagtgagcat tttccttgcc gttaaacgga
    13201 gcgaagtgaa agcccattga ataaggaaag ctgatgttaa acagattgtc gtaacgggtg
    13261 gtgagctttt tcaatgcaag ggctaaatcg gctttttccg cttcgtttaa atcggtaatg
    13321 gatttgaact gcttggcttt cggcaacagc aaggtttcaa acggccaagc cgcccagtaa
    13381 ggcacaaccg caatccaatc ttcggtttct gccacaattc gctcttgttt gtctaattca
    13441 cgttgtgcgt agtcaagcaa cagtggcgag ccatattgag caaaatattt cgcttggcat
    13501 tcgtcttcaa tcgcaatttc attaggtaga aaattacttg cccaaatttg cccgtgtggg
    13561 tgagggttag aacaccccat cattgagcct ttgttctcaa aaatttgcac ccactgataa
    13621 tgctctttca gctcattcgc ttgctcttgc caaactgtta ccacatcagt aatttcctcg
    13681 atggaaagtt gcggtaaggt tttactatga tcaggcgaaa aacagatcac acggctttcg
    13741 ccttgtgtat gagaaatctg aaacagcgga tcatcattcg gtattggtgc cggtgtgtct
    13801 ggtagcaggg cagagaagtc gtttttaaac acaaacggct tggtgtaatt cgggttttgt
    13861 tcgccggtaa tccgcttatt gccggggcag aggtaacaac ttgggtcgta gctcggtttt
    13921 tcatcttgca cgatttcttc ttgttgccct tgccacgggc gttttgcccg atgcggagag
    13981 actaaaatcc attgattttt taatggatta aaacgacgat gtgggtgatc gttgaggaca
    14041 aattcgctca tataaaactc ctcgaaatta gttatgtaaa gagtatagcg aaagtcagac
    14101 aaaatgactg tgacatagct cacaaaataa tgatgtcaag cggtcaaatt tcagaaaaat
    14161 tttgcaagtt ttcctccatt ttgcgattta aggtagctta cgtggaattg aatgtggctt
    14221 ttggtagaat agatgaatat gcaagatatt aagacaaaaa tagatggaaa gactgaataa
    14281 atttgatgct tggttaatga atcataaacc taatgctagg ttaagcggag cgagtcgttt
    14341 gttgacagag ttttggttct ttgggttaaa agaagctaga gcctgtttat ttgcgggatt
    14401 attttttatt gcgatgtttt cggtaccaaa aacgggatta tggggcttac ctcgttatga
    14461 tattttgctg atttttgcca tagtggtgca aattgcaatg gtgaaactgg ggctggaaac
    14521 gtgggatgag ctaaaagcaa taagcctatt tcatttggtt ggttttgtgt tagaagtctt
    14581 taaaacatcg agtaatattc aatcttgggc atatccggat tttgcctata ctaaattgtt
    14641 caatgtgcca ttatttactg gttttatgta tgcagcagtt ggttcttaca ttatccaagc
    14701 gtggcggttg tttgatttga aaattaaaag ccacctgccc tattggttag ccactttaac
    14761 ggcactgatg atttacctga atttctttac gcatcattac attggggatt accgttggta
    14821 tattgccgct tttgcattag ggctgtatgc acgtactttt gtatttttca ccccttatga
    14881 cattacacga aaaatgccac tgctgctttc tttcttcctg ataggttttt tcatttggtt
    14941 ggcggaaaat atcggtacgt ttgccggaat ttggcgctac ccgaatcagt ttggggcttg
    15001 gtctatggtg catatgagca agtggagtgc ttgggcgtta ttggtgatga tgacttttac
    15061 catcattgtg catttaaagc atatcaagca aacagtcaga gtctcgagcg attaacaagc
    15121 tgtgggtttt ggcgaaaatt ttactcatca gttattttca gtggaattag gggcaaataa
    15181 taatttgccc ctatatcaaa ttatggattt tccccttcca catcaataat catcacttcc
    15241 gactgtgagc ctaaccgcat cggcacgccc cagaatccgt agccggaggt gacaaaataa
    15301 tgcccgaagc cgattttttc atagccgtaa tgtaatcgat agatcagttt ggtaataata
    15361 cttgcaggga acacttgccc tttgtgagcg tgtcctgaaa cttgaatatc aatcggcaat
    15421 ttagcatgtt gctcaatttc agtcgggcgg tggtcaagca aaattaccgg tagtttagtg
    15481 ttcacgtttt gtaaaagctg ttcggcagaa ggacgatttt tgactaaatc atcattacgc
    15541 ccaataaggg taaattttcc gttaatttcg gccgtttgat cccataaaac agtaataccg
    15601 gcgtcggtaa tttcttttgc aatcgtttct tgagccccaa aaaagtcgtg attgccaagg
    15661 gtagcataca cgccaagagg agcttttaat ttggctaaat gaggctgcat tttatcggca
    15721 acataaactt cggtattgtc gtccataata tcgcccggca ataaaatcag atcgactttt
    15781 tgctggttga aaatctctgc cagttggtca agttgcttgg ttccgaacag ttttcctaaa
    15841 tgcagatcgc ttgccatacc aatgcgaagc ggtttctcta atggtttatc caatttcacc
    15901 gaataatgta ctactgttgg ggtataagcg ttgtaatagc caagcccaag taagccaaag
    15961 aatgcaaatg ggtaggcgat tttcagccag cgattgacgg tagtattgcc gttgaatatt
    16021 ttgtaaatac aagcggtgaa aaaactgata aaaaatgcaa aaagtaacaa taccaatatg
    16081 aatgccatta ttctaaatag cgcaaaagta cgagtaagat gcaaaatcac taatccgttt
    16141 gcgactaaga aagtgagaat accaactgtt ttacggtttt tacttgataa attaaataac
    16201 cagctgacag tctttgcaaa gttgaataaa aagagttgta acaccgcaat cacaagggcg
    16261 gtggtaatgt agtaacgata ttccatattt ttccttcaat aaattggcta aacccagccg
    16321 atttgtctta ataaaaataa gccgatgctg gcactaaaca ttgagccgag agtcgtaatg
    16381 ccgatcagat ttgcggctgc ctcgccgtct ccgccatagt tgcgtgtcat tgcgtaggtg
    16441 gctgaagcaa cgggagcaga agcagccaca aagacaatgc ctagttccat cggcggaagt
    16501 tgaaacacaa atttcccgag tagtagcaag aaaattggag caaaaatcag ccgaatcgag
    16561 gcagaaaaca gcacaatttt attgatggtg ctttccactc cttgattaaa ttgtttcaac
    16621 tgtttgaaat taatgcttgc tccggtgcag ataagtgcta atggtagtgt caggctcgcc
    16681 attaatttac cagcagactc aagcggggtt ggaatttggt ggcttaattc aaaatagtta
    16741 aaagttaagc ctgcaatgac ggctagaatc agcggatttc tcaatatcga caatattaat
    16801 ctgccaaaat tcactttttt ctcgcttaac gatgaagtga gggtaatcac tgcaagcaca
    16861 ttaaaggtaa tcaccaagca cgaaatataa actgaagcgg cagcgagagc tttatcgcca
    16921 taagcattga gaattaaggc aatgcctaaa atcgccgcat tggtacggaa agtgccttga
    16981 gtgaaaatac aacgataacg gcggttttca atatgtcgag ccgcccacca ttccgcccat
    17041 aaatagatca aaatcgaacc gccgactccg gtcgagatca ggttgatttg gctggagaaa
    17101 tctaacgggc tttttagcat attcaagaaa atcatcggtg gcagggtaaa attaaatacc
    17161 acctttgatg ccatattgca aaaggtatcg tcaataaatt ttcgtctgcg tggaaaaata
    17221 ccgaacagca ttaacaaaat ggttggtaag gtaacgccaa tgctaaattg gatagagtcg
    17281 aaaagcataa tgtttgcctt aaatgatggc acgagtgtct tttgcttgta ccgtagatta
    17341 tcaataaagt accagcgtgg acgctggtac tatcatcaat ggaaatgaaa ttacaatcct
    17401 gcctttaagc ttgcttcgac aaatttatcc aaatcaccgt ccagcacttg ttgggtggtg
    17461 cgatgttcca caccggtacg gtggtcttta atccgtgaat cgtctagcac ataagagcgg
    17521 atctggctgc cccagccgat gtcggatttg ttgtcttcca tcgcctgttt ttccgcattt
    17581 ttcttgctca tttccagctc atatagcttc gctttaagct gtttcatcgc ctgatcttta
    17641 ttttgatgtt gtgaacgacc gttctggcac tgcaccacaa tgccgctcgg tatatgggta
    17701 attcgcaccg ccgattcggt tttattgacg tgctgaccac ccgcccctga ggcacgatac
    17761 acatcaatcc gcaaatccgc agggttgatt tcaatatcaa tatcgtcatc gacttccgga
    17821 taaacgaaag ccgccgagaa cgaggtatga cgaccgttat tggcatcaaa cgggctttta
    17881 cgcaccaagc ggtgaatacc cgtttcggtt ctgaaccagc cgtaagcata gtcgccggaa
    17941 attttcacgg tggcggattt aatccctgcc acatcaccgt ctgaggcttc cattagttcg
    18001 gctttaaacc ctttgctctc tgcccaacgc aaatacatac gcagtaacat ttctgtccag
    18061 tcctgagctt ccgttccacc cgaaccggct tgcaaatcca aatagcaatc ggcactgtcg
    18121 tgtgggccgc tgaacatccg cttaaattct aaatcggcaa gttttttctc taattcgtct
    18181 gcttccactt gggcttcatt gaaggtatcc tgatcttctg cttccaccgc cagctcaatc
    18241 aacccttcaa catcttcaat cccttgctct aaggctttaa tcgtattgac gatctgctcc
    18301 aacattgccc gctctttacc aagcgcttgt gctttttccg gcttgttcca tacatcgggt
    18361 tgttccagct cggcattgac ctcttctaag cgttcgactt tatgctcaaa gtcaaagata
    18421 ccccctgatc accgcggtgc gctcgcttaa atcagttagt tggtttttaa ttgggtttaa
    18481 ttcaaacatt gtcgttcctg tttttgctta ttttaaaaag atgaattata gctgaagtta
    18541 agcaagcggt cgaattttgc aagaaatgga gcaatgatcg tttctagtaa tgagatttgg
    18601 ttcataaaag ttgcaactgt ttctgctatt tggctttgat agctaaaaat ctttaaaaaa
    18661 acaagcggtc gttttaccac attttttgca aaacgaccgt agcttcttgc tataatcgct
    18721 gaaaaaatgc tgcaaggaga ttttatggca aagcgcccaa gtgatattga tgcaacccta
    18781 ttgcaactgg aaatcctacg ccgtatcccc aaattacccg ctaaaattga tgctaaaagt
    18841 ttgcaccaac agctattaga tgccggcttt gaacgggata ttcgcactgt tcagcgttcg
    18901 ctcaaaatgt tatgcgagca ttttgatatt gaatgcgatg agcggagcaa accctttggt
    18961 tatagctgga aaatccgctc agaggggctg gcattgccga ttctcaacga acaacaatca
    19021 ttactgctaa aacttgccga gcaacagctg aaatatctgt tacccgcaaa tttaatggca
    19081 tcaatgaaac ccttttttga gcaggcagac agaatggttt ccggggcgaa agacaagccc
    19141 gaataccaat ggctaaataa agtttgctcc acgccaacca gcctaccgct gatccctgca
    19201 aaaattaaag aagagatttt taattccgta agccaagcct tatttcaaaa taagttgtta
    19261 aagatcgaat atcagaacca gcacggcaaa aaacaccaag cgacaattat gccgttggcg
    19321 atttcacaac aaggtgcgag catttatttg gtctgccgtt acgaagggtt tgacgacaac
    19381 cgcctgttgg ctcttcaccg cattcgtaaa gcggagattt ctacttttag ttttgaacgc
    19441 ccgaaagatt ttaatttgca agcctaccaa gatgccggac acctcggttt tggcaatggc
    19501 gaaaaagcta gccttacctt ttcgattagc agagacgccg gtttccattt aaccgaaaca
    19561 ccgcttgcca aagatcaaaa aattctcgag gagagtgaaa ctcactaccg tattcaagcc
    19621 atagtcgctg ataatgatat gctaacctgg tggctacgca aatttggcga agatatttgg
    19681 gatatcgaga aagaagcgct aaaataagct aaacaagcgg tcggttttgt gggaattttt
    19741 gctaaatagt atcgaccgct cctccctaaa cagaccttta tcataaatac aactctttca
    19801 atcagatatt cttactgtct attgatgaac gattcgaatt tttctagtgg ttgaaattta
    19861 aactaatttt cttgcttttt ggcatttttc agacttttaa ttctctccat tagcctgttt
    19921 ttttacgctc tttttcataa acaggaataa ccaatgcctt ttcaacttac tttctgccaa
    19981 caagccggca atcagcacca gaaaaatcaa gatgcactgt ttaacggcaa agcggtgtac
    20041 caatggcaac ttaaaaatgc cgagagttat ctgctggata acgacaaagt aatttttggg
    20101 gttgcggacg gcgtttccag ccaccctcga aatcatcttg cctcacatta tttaatggcg
    20161 ttactcagcc aatgccaatc ccttgatagc caatggttgc gacaggctca cggcgatttt
    20221 tgcgaaaaat tagcgaaaga ttattttggt gcttcttgca cttttgttgc ttgtgaatta
    20281 caccataacg gcagagggcg tatggttaat gtgggcgaca gtcgagccta taaaattacg
    20341 gcaaacgggg agtggcaaca actgagtttt gatcacacca tactggctga aatgaaacag
    20401 aaaggcttgg ttaaggaagg gagcaactat gcctcaatgt ataacggctt aaccgattgt
    20461 attgttgccg attttgacgc cgatgatttc cgcattcata tagccgaatt tcaactggaa
    20521 aaaggcgaga gcattttgct ctgttctgac ggtttgcacg aacaactcgc cccagaagct
    20581 attcaggcga tttaggacgg atacgaaagc ccgtctcatc gtctgcaaat ttgcagaaaa
    20641 atggtaaaaa tgcaccgctt gtatgacgat ttttctgcgg tgatttgtga aattgtgtaa
    20701 ttaaaacgac attatttgtc gcattggtgc ttaaaatccg cataaaacag aggagaaaaa
    20761 aatgaaaaac gatttccatt atgccgctga attaatccgc aatgcagacg gcattttaat
    20821 taccgcaggg gctggaatgt cggtggacag tggcttgccc gatttccgta gcgtgggagg
    20881 gttatggaat tcctacccac cgctcaaaaa atttaatttg caatttatgc agattgccac
    20941 accgcttgcc tatcaggaac gccctgagtt ggcaaaatgg ttctatgccc atcgcctaaa
    21001 tcaataccgc caagccgaac cgcaccaagg ctatgccatt ctcaaacgct gggcggaaag
    21061 caaacaacac ggctatttcg tcttcaccag caatgtggac gggcatttcc gcaaagccgg
    21121 tttttcggag gatcggattt acgaatgcca cggcacgcta gaccatttgc aatgtttggc
    21181 aaattgcaaa aataaatcgt ggtgggcgta cgactatcaa cctgtaactg acgatgaaca
    21241 ttgccttgta ttgagcgaac caccaacttg ccctgactgc ggaggattag cccgccaaaa
    21301 tgtactgatg tttaatgatt ggagttatgc cgaaggctac caacttacta aagagacact
    21361 gttagatgaa tggctacgca aagcccaacg ccctgtggtg attgaaattg gtgcaggcaa
    21421 agtcgtacca actgtgcgta atttttccga acgcaccgcc aagcagaaaa aaggtggttt
    21481 aatccgcatc aacccaatag atgccggcgt acccaaaatg cactttttaa gtttggaaat
    21541 ggggggatta gaggcgttgc aggggattga ggaatgctta taagcggtct aaattcacca
    21601 agactttgca aattttgcta caataaaaac attgaaatat tgtaataaag gatctgaaat
    21661 gagccaaaat aatacagaaa aagaaattgc ggaaattaga aaaattgaag atgttgtaag
    21721 aacgaaatta tctattcctg attatcagcg cccttataag tggtcggtaa aacatattac
    21781 ccaattatta aacgatctct attttcattt tgaaaatggt aagaaagttt atcgagtagg
    21841 ttcggttgtt atccatacat atacaggtaa agatgagaat agtgagtcta aaacaaaatt
    21901 cgatatagtt gatggacaac aacgtttaat tagcctttct ttaattttat ttttattgag
    21961 caatgataac acttttccac ttttatctga aaagctctca catagtatta gtgcggataa
    22021 tcttataaaa aataaggttg cagtagaaca atttattcga gatagagtac aagataaaca
    22081 ggaatttgct acctatattt tagaaacttg cgagtttgtt tatattgaat taggggatat
    22141 tgacgaggct ttccaatttt ttgatgctca aaatgcaaga ggaaaatcgc ttgcacctta
    22201 cgatctatta aaagcgtatc atttaagaga acttaaagca gataaagaga cgatttatca
    22261 atgtgtagag aattgggagc aagcagtaga agctgagatt gctgatttaa aagaaatcat
    22321 cagtgagacg ttatttagat tacgccgttg gcaaaaatat caaacagcag aaaaattcac
    22381 gaataaagaa ctcgatacat tcaaaggtgc tagctcggat aaaa
//
LOCUS       DANJWC010000021        21883 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.021, whole genome shotgun sequence.
ACCESSION   DANJWC010000021 DANJWC010000000
VERSION     DANJWC010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 21883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21883
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.021"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..829)
                     /locus_tag="PWX02_000598"
     CDS             complement(<1..829)
                     /locus_tag="PWX02_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDL1728636.1"
                     /translation="MQSYPYLLVNVFATSHFGGNPLAVFYEADGLTDLQMQLIARQFN
                     LSEVVFIQSSTDSQAVKKLKIFTPDYEMPFAGHPTVGAAFVLHSAFDFPTTYRLQTNA
                     GLVAIQHQHNVTTFALTNGVNLENTPLSRAECASILGLNVADIASEPCYVNTGVEQLL
                     VELASQQAVEKCKINANLAEKAFLKDSVYVWCREGNEAKVRLFFQSQGSIVEDPGTGS
                     AAANLGGWHIAQGKIPIQLQISQGDEIQRPNRLLLKVDQNGKIFVGGEVILVGKGEFY
                     "
     gene            1018..2352
                     /locus_tag="PWX02_000599"
     CDS             1018..2352
                     /locus_tag="PWX02_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDL1728637.1"
                     /translation="MLYAEFLLLLAFLYAGSRYGGIGLGVVSGIGLFVEVFFLGMPLS
                     APPISVMLVILAVVTCASILEAAGGLKFMLQVAERILRSNPKRITFLGPLVTYLMTLM
                     LGTGHSVYSIMPIIGDIALKNKIRPERPMAAASVASQLGITGSPLSAAVAYYLTEIVK
                     LPGFEGVTLLNVVGVTITATFCGVIAMSLYSLRRGKELEDDPEYQRRMQDPELRKQIE
                     QTSATSLDEKLPASAKNSVYLFLAAIATIVIIAMIPAIKPTVAATGKVAGMDQIIQIV
                     MLTFGGLILILTKTDPKKVPNGIVFKSGMVATIAIFGIAWMSDTYFTHAMPAFKEGVT
                     EMVRAQPWTFAFALFAVSVVVNSQAVTAKMLLPVGIAMGLPAPLLVGLMPATYAYFFI
                     PNYPSDIATVNFDVTGTTKIGKYYFNHSFMAPGLVGVITACIVGIAVANVLI"
     gene            complement(2432..2842)
                     /locus_tag="PWX02_000600"
     CDS             complement(2432..2842)
                     /locus_tag="PWX02_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDL1728638.1"
                     /translation="MNSTLTNLQPLAFLITRVVAGYMFLLHGTAKFFEFPMSMTNGNG
                     SVPLASIYGVGGILEIVGGIFFILGLFTRQTAFILSGMMAYAFFIAHGAQGNVLLPLL
                     NGGELAGVYSVLFLTYIFIGGGKYSLDAKLFNKA"
     gene            complement(2965..3642)
                     /locus_tag="PWX02_000601"
     CDS             complement(2965..3642)
                     /locus_tag="PWX02_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL1728639.1"
                     /translation="MNLFGFRCFHCNHPLAIGVQGICSHCQKLLKPTPYCRHCGMSAV
                     EFRNACGYCLKNEPKWHKLVRVNEYRAPLSHWVHQFKFQQQYWWDQALARQLLLAIKQ
                     AQREQVLSLPEVIIPVPLFWHRHWKRGFNQSELLCDWLSKWLQIPLDTQSLQRVRATV
                     SQRELTAAERRKNLRGAFFYQPLKAYKRVAIVDDVLTTGSTLNVICAELLKAGVVEIQ
                     VWVLARA"
     gene            3760..4572
                     /locus_tag="PWX02_000602"
     CDS             3760..4572
                     /locus_tag="PWX02_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL1728640.1"
                     /translation="MKSLQKPIKIVFFDIDDTLYVKHKAYIPKSITEQVLPRLKAKGI
                     IPAIATGRNHGCFPEALKPYLNPEIGFELFVTINGQYNFYKDQIISDYSLSREQIERI
                     IEKANALGILYAFITPMTIAVSETNPIVHEAVLPITPNYLIDPHYYEKEKIVQMLLFY
                     TEEQKQAVENSGIFANDLKEARWHPNAVDILRKENSKARGIQDVLATLNIEIESAMAF
                     GDGINDLEMLPTVGVGVAMGNGVPELKAVADFITKPLEEDGILYALETLEVI"
     gene            complement(4620..4889)
                     /locus_tag="PWX02_000603"
     CDS             complement(4620..4889)
                     /locus_tag="PWX02_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDL1728641.1"
                     /translation="MGLLYLLLLNTLIGYGSYGEALNHWDTSKVSIVTTMLPIFTMIF
                     SNLSYYFYPHIFAKPDMNWISYLGALVVVSGAILAIAGDKLFRRN"
     gene            complement(4862..5539)
                     /locus_tag="PWX02_000604"
     CDS             complement(4862..5539)
                     /locus_tag="PWX02_000604"
                     /inference="COORDINATES: protein motif:HMM:NF013088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL1728642.1"
                     /translation="MKQRPLLGFSLALTAAMTWGTLPIAAQQVLKVIDAQTLVWVRFV
                     VASVGLLLILGFAKRLPKLTACSKKELGLILLGVCGLSANFFLFAEALHYVSPTINQV
                     LWQLAPFSMILLGVLLFKEKFGLFQKIGFVLLIAGLVAFFNDKFAEILQLNTYALGVL
                     LSAGASLIWVLYGVAQKLLLAKFNSQQVLFIIYIGCSLLMLPFASPAQLGNLEDGFLW
                     GCFIYCC"
     gene            complement(5641..5898)
                     /gene="rpmA"
                     /locus_tag="PWX02_000605"
     CDS             complement(5641..5898)
                     /gene="rpmA"
                     /locus_tag="PWX02_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDL1728643.1"
                     /translation="MATKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGSNVGMGKDHTLFATADGKVKFEVKGEKSRKYVSIIAD"
     gene            complement(5919..6230)
                     /gene="rplU"
                     /locus_tag="PWX02_000606"
     CDS             complement(5919..6230)
                     /gene="rplU"
                     /locus_tag="PWX02_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDL1728644.1"
                     /translation="MYAVFQSGGKQHRVSEGQVVRLEKLELATGEKVEFDSVLMVVNG
                     EDVKIGAPVVAGAKVVAEVVAQGRGEKVKIVKFRRRKHSRKQQGHRQWFTEVKITGIQ
                     A"
     gene            6508..7506
                     /gene="ispB"
                     /locus_tag="PWX02_000607"
     CDS             6508..7506
                     /gene="ispB"
                     /locus_tag="PWX02_000607"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HDL1728645.1"
                     /translation="MAVQLFSLEQIQALANEDMQAVNAEILAQLNSEVLLINQLGHYI
                     IAGGGKRIRPLIAVLAARALGYRGKKHITAAAFIEFVHTATLLHDDVVDESDMRRGRP
                     TANAHFGNAASVLVGDFIYTRSFQMMTDLDSLRVLKVMSAATNALAEGEVQQLMNVND
                     PNTTEANYMRVIYNKTARLFEAATQCVAIVADASEEIETALKNYGCYLGTAFQLIDDI
                     LDYSAQSESLGKNVGDDFVEGKPTLPLLHAMHAADKAGKTTQAELIRNAIENGGELEQ
                     IPQVLAIMQEHQSLDYAMQKAREEAQKAVDSLGVLPESEYKQALVSLAYLSVDRSY"
     gene            7609..8364
                     /locus_tag="PWX02_000608"
     CDS             7609..8364
                     /locus_tag="PWX02_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDL1728646.1"
                     /translation="MTEQNQQNQQPQEHIESVRARRRAQNQKKDPNAPFVREKLTLPN
                     GHNKLLLHSCCAPCSGEVMEAIHASGIEFTIYFYNPNIHPLKEYLIRKEENIRFAEKW
                     GIPFIDLDDDYENDRKEWFKKAKGMEWEPERGIRCTMCFDMRFEKAAEYAHNNGFPVF
                     TSCLGISRWKDMNQINDCGHRAAAKFDDVVYWDYNWRKGGGSQRMIEISKRERFYQQE
                     YCGCVYSLRDSNKWRLETGRQRIEIGKLYYSPD"
     gene            8781..9716
                     /gene="fabD"
                     /locus_tag="PWX02_000609"
     CDS             8781..9716
                     /gene="fabD"
                     /locus_tag="PWX02_000609"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL1728647.1"
                     /translation="MSKFAMVFPGQGSQAVGMLADLAPQYPIVEETFKQASDVLGYDL
                     WDLVQNGTAEELGQTHRTQPALLASSVALFRIWQQKFPEQKPAVMAGHSLGEYSALVC
                     AGVLDFQDAIKLVELRGRAMQEAVPAGSGAMFAIIGLDNESIIKACEQAASETGEIVS
                     AVNFNSPGQVVIAGTKAAAEKAGELCKAAGAKRALPLAVSVPSHCALMKPAAEKLAEA
                     LQNIELNQPLVPVINNVDVAVETDADTIRNALVRQLYSPVRWTETVEKMAQDGITTLY
                     EIGPNKVLTGLAGRIVKELSAKAVNDVASFEAVEA"
     gene            9733..10197
                     /locus_tag="PWX02_000610"
     CDS             9733..10197
                     /locus_tag="PWX02_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728648.1"
                     /translation="MSKETLFMLISALIALVILPLAYTVGIAKETLGGNMGMLSIAAG
                     CSFLAYFLSLNPKIRHAFIGGSAITAAAIQAWVLYGVLLSENDPKSAVVLFQIYLFSS
                     LGPLFFSAIWAFTRPRWIYPKQGSAVKYALFGGLTTVINYLITLFIFYILTL"
     gene            10218..10940
                     /gene="fabG"
                     /locus_tag="PWX02_000611"
     CDS             10218..10940
                     /gene="fabG"
                     /locus_tag="PWX02_000611"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDL1728649.1"
                     /translation="MSKIALVTGATRGIGKAIAEELVAKGYIVVGTATSEKGAESISA
                     YLGENGKGLVLNVADAESIESVLKQIKETFGDVDVLVNNAGITRDGLLMRMKDEDWFD
                     IIQTNLTSVYRLSKAVLRPMMKKGGRIITIGSVVGSMGNSGQTNYCAAKAGLVGFSKS
                     LAKEVASRGITVNVVAPGFIATDMTDELTEDQKNAILSQIPAGELGSPQDIAKAVAFL
                     ASDDARYINGETIHVNGGLYMS"
     gene            11053..12351
                     /gene="brnQ"
                     /locus_tag="PWX02_000612"
     CDS             11053..12351
                     /gene="brnQ"
                     /locus_tag="PWX02_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL1728650.1"
                     /translation="MTRNTLVVGFLLFAIFFGAGNLIFPPKLGYESSTEFFSAISGFV
                     LTGVGLPLLGIIVSAFYNGGYKSALDKIHPWFSLAFLTSIYLAIGPFFAGPRTAATAY
                     EMAIMPFLGDSSQVSMLIFTLIYFAFTLWLALNPNKMVDRIGAILTPILLITIIGLVI
                     RAGFILDTDPQITTPNTNEQSYFFKGMVDGYLTMDALASLAYSVIVLNAIRGKGVSQA
                     ALQRQTVSAGLVAAVALAVIYISLGWIGNNMPISADTLSMLSERKLDLGTYILNTVTT
                     QAFGELGRSVLGMIVSLACLTTAVGLAVSVSEYFNEIFPKISYKVYAILFTVISFTVA
                     NQGLKEVISKSIPVLLVLYPIAMTVILMLLINLVIKLPLSAQRLSLLLVTIVAIASVA
                     GVGFTENLPLKSYSMEWLPFAIIGAIIGAIIHPLTGRKHK"
     gene            12397..13221
                     /gene="mutM"
                     /locus_tag="PWX02_000613"
     CDS             12397..13221
                     /gene="mutM"
                     /locus_tag="PWX02_000613"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDL1728651.1"
                     /translation="MPELPEVETSVRGVSPYLVGQTIEQIIVRQPKLRWAVSPELSQM
                     AGAKITAVERRAKYLIIRTDKGDILVHLGMSGSLRILSQTADIAFGKHDHIDLVTTNG
                     TILRYNDPRKFGCWLWAEKAEDHELLRKLGPEPLSNDFTADYLFAKSRKKTTACKTFI
                     MTNEVVVGVGNIYACESLFMAGIHPELAAQNLTKKQCERLVKTIKEVLTKAIIQGGTT
                     LKDFIQPDGKPGYFSQVLQVYGRKGEACNDCGKTIETKVIGQRNTYFCPKCQRLPK"
     gene            complement(13304..13738)
                     /gene="dtd"
                     /locus_tag="PWX02_000614"
     CDS             complement(13304..13738)
                     /gene="dtd"
                     /locus_tag="PWX02_000614"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDL1728652.1"
                     /translation="MIGLIQRVKFAKVEVEGQVVGEIASGLLVLLGVEKDDDEAKADK
                     LLEKVLNYRVFSDSEGKMNLNVQQAGGSLLVVSQFTLAADTNKGLRPSFSKGASPVDA
                     ERLYAYFHQQAATKIHTQTGQFAADMQVSLQNDGPVTFWLQV"
     gene            complement(13713..14558)
                     /locus_tag="PWX02_000615"
     CDS             complement(13713..14558)
                     /locus_tag="PWX02_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HDL1728653.1"
                     /translation="MLNRLWRFIKLFAKRYNENNIAISAGYLTYSTMLAIVPFIVVLF
                     SIFTFFPIFHEANEQLKAFIYDNFAPSASEMVSEYIELFVSNAKQMGLVSFIGLFVVT
                     IMLIKSIDEALNTIWHDNRQRPMWFSFLLYFLILLFGPLLAGASIAISTYVMSLSIFN
                     GSGALSLGQHLLELTPFLLIWLLFTLVYKFIPIRKVKFIHAAIGALMAAIFFTLGKKA
                     FIWYITAFPSYQAIYGALAVLPIMLLWIQLSWQVVLLGGQFAAVLDEMETDKGSVNDR
                     LNSAG"
     gene            complement(14560..15798)
                     /gene="proA"
                     /locus_tag="PWX02_000616"
     CDS             complement(14560..15798)
                     /gene="proA"
                     /locus_tag="PWX02_000616"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDL1728654.1"
                     /translation="MQMIELAQQAKQASFELAQFGNLQKNQALLTIADSLEQRAEEIL
                     YANAKDIEFAQAQGISLAIIDRLLLNEDRLKDIANDVRNVAKLADPVGQVMDGGVLDS
                     GLKIERQRVPLGVILTIYEARPNVTIDVASLCLKTGNAVILRGGKETKFTNAVLVDVV
                     QDALEKAGLPKLAVQAVTDPDRALLLELLKLDRYIDMVIPRGGAGLHQFCKENSTIPV
                     IVGGIGVCHLFVEKTADQEKSLEVIANAKTQRPSTCNTLETLLVEKAIAVEFLPKLVA
                     KMQQLGVTIHSNDLQKIEGIEPLDQAKLSQEWLSLDLNVVVVNDLQQAVEHIRHYGSQ
                     HSEGILTSDYQLARQFVQQVDATAVYINASTRFTDGGEFGLGAEVAVSTQKLHARGPM
                     GLEALTTYKWVCEGDYLVRG"
     gene            16082..17965
                     /gene="mutL"
                     /locus_tag="PWX02_000617"
     CDS             16082..17965
                     /gene="mutL"
                     /locus_tag="PWX02_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDL1728655.1"
                     /translation="MINTTPKQLIHILPPQLANQIAAGEVVERPASVVKELVENSLDA
                     GANTIQIDIEKGGSQLIRIRDNGCGISKQDLPLALARHATSKISSLEDLEAILSLGFR
                     GEALASISSVSRLTLTSRTAEQTEAWQAYAQGREMAVEIQPASHPIGTTIEVANLFFN
                     TPARRKFLRTDKTEFAHIDEVVRRIALAKPNISFSLSHNGKIVRQYRKVQDNSVEQQQ
                     KRVAAICGDEFIQHSTYIDWQHGDLHLHGWVGSPALARLQNDLCYSYVNGRMMRDKTI
                     NHAIRQAYGDSIASGYYPAFVIFLDLDPTMVDVNVHPAKHEVRFHQGRLVHDFILQGV
                     LNSLQQTQPTLELRNEVNEPLPSYIQDTNRQAAGQNIFVSQTAPQAAEFTQKNASRSS
                     YTPKFERSNSVSSSAQKWYKELVGGDLHQTTPTIKTQSTLVEPKTEPQTEHKTEAVSQ
                     KITQIAPLASHHSQALAIVKNQAVLIKEEENFYLLPLATLAELKLKLQLEKGQSQALL
                     IPLNLSLDQQQTERWQQTKEELTELGFSISEKQWQAQTRLTVLSVPQAVREQNLQQLL
                     FALFNQTQAVNLAEFFAKKCEKPTACTLSDVIALLSEIELYANGKKELEGIRKEVDFS
                     KFL"
     gene            complement(17984..18253)
                     /locus_tag="PWX02_000618"
     CDS             complement(17984..18253)
                     /locus_tag="PWX02_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HDL1728656.1"
                     /translation="MREIEYSNAFKRDYKKCAVESLSSSLIEALYFLINDKPLPEKYC
                     DHALTGEWKGFRDCHIQPDLVLIYRKVEDDKLELLRIGSHSHLFG"
     gene            complement(18240..18506)
                     /locus_tag="PWX02_000619"
     CDS             complement(18240..18506)
                     /locus_tag="PWX02_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL1728657.1"
                     /translation="MANSALVSVRISPEIKEQASEVLAGIGLTVSDVMRMTLAKIATE
                     KRFQFDYQPNFETAEVLRAVKNGEEKLHSANSIADLMEQLNARD"
     gene            18952..19905
                     /gene="miaA"
                     /locus_tag="PWX02_000620"
     CDS             18952..19905
                     /gene="miaA"
                     /locus_tag="PWX02_000620"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDL1728658.1"
                     /translation="MNKKPLALFLMGPTASGKTDLAIALRQELPIEVISVDSALIYKG
                     MDIGTAKPSKEELALAPHRLIDILDPAESYSAMNFHADALREMADITSQGKIPLLVGG
                     TMLYYKALLEGLSPLPSADPAIRAEIESRAAQHGWATLHQELVKIDPIAGARINPNDS
                     QRINRALEVFYITGKTMTGLTAQQGEALPYNILQFAIAPQDRAVLHQRIEQRFHKMIE
                     LGFEEEVKRLFLRKDLHINLPSIRCVGYRQMWEYLQGDISLDEAIFKGICATRQLAKR
                     QITWLRGWQGELTWLDSLAPTSSKAKMVEKIDHYLKNCDNI"
     gene            19974..20246
                     /gene="hfq"
                     /locus_tag="PWX02_000621"
     CDS             19974..20246
                     /gene="hfq"
                     /locus_tag="PWX02_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDL1728659.1"
                     /translation="MAKGQSLQDPYLNALRRERIPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPARALSHNNNNQAQHAQPAAEVTEQAE"
     gene            20266..21651
                     /gene="hflX"
                     /locus_tag="PWX02_000622"
     CDS             20266..21651
                     /gene="hflX"
                     /locus_tag="PWX02_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDL1728660.1"
                     /translation="MLNESLPNSEENSAFGFALSEASQTTEAASPTDPENQDKAILVH
                     LYLGQHKDVENLLEFKTLAESAGVEVLATLTTSRQSPHIKYYVGQGKAEEIAEAVEQL
                     GATVVLVNHQLSPAQTRNLQSLCGCRVVDRTGLILDIFAQRARSHEGKLQVELAQLRH
                     LSTRLVRRVTNQDQQKGGAVGLRGPGETQLETDRRLIKVRIQQLLNRLEKVSKQRNQN
                     RQTRQKADIPTVSLVGYTNAGKSTLFNAITQAGVYAADQLFATLDPTLRRIQVQDVGT
                     TILADTVGFIRFLPHDLVSAFKSTLQETTEATLLLHVIDGSDERKNENIDAVNQVLDE
                     IGALDIPTLLVFNKIDKLEDVEPHIERNADAMPVAVYLSAQENSGIELLFQAIQERLR
                     SNLVCENLLLPVTAGAIYAQFKTENCIKTEHFNEFGERMIYIEINEVQWNKWVKQFPD
                     LAEYIELAKWG"
BASE COUNT         6548 a         4828 c         4495 g         6012 t
ORIGIN      
        1 aataaaactc gcctttgccg actaaaataa cctctccgcc aacaaaaatc ttcccgtttt
       61 gatcgacttt taataacagg cgattcggcc gttgaatttc atcaccttgt gagatctgaa
      121 gctggattgg tattttgcct tgtgcaatgt gccaaccgcc taaatttgcc gctgccgaac
      181 cggtgccggg gtcttcgaca atcgaacctt gcgattgaaa aaacagtcgc actttcgcct
      241 catttccctc acgacaccaa acatatacac tgtctttcaa aaatgctttt tcagccagat
      301 ttgcatttat tttgcacttt tcgaccgctt gttgagaagc taactcaacc aataactgct
      361 ccacgccggt gtttacataa caaggctcgg acgcaatatc cgcaacattc agccccaata
      421 ttgaggcaca ttctgcacgg cttagcggtg tgttttccag gttcacacca ttggttaaag
      481 caaaagtagt cacattatgt tgatgttgaa tcgctaccaa tccggcatta gtttgcaagc
      541 gataggttgt tggaaaatca aaggcggaat gcaacacaaa agccgctcca acagttgggt
      601 ggccggcgaa cggcatttca taatcaggcg taaaaatttt gagttttttg accgcttgtg
      661 aatcggtgga agattgaata aataccactt cagaaaggtt aaattggcga gcaataagtt
      721 gcatttgtaa gtcggttaat ccgtccgctt cataaaatac tgcaagcggg ttgccgccaa
      781 aatggcttgt tgcaaacaca ttcaccagca aataaggata gctctgcatt tatcactcct
      841 tacataaact taaagccaca ttgggcgaaa attctaccat tttggtggcg aatcgtaaaa
      901 ttggaaaatt tatcggtatc cctctttaga gttaactatt tttgatttat ttcaaagttt
      961 taaaaaaaga ttaggttata cttctttcta gtgattattt tttaatttga ggttcttatg
     1021 ctttatgcag aatttttact tttattagcc tttttatatg caggtagccg ttacggtggc
     1081 atcggtttag gtgtggtttc cggcatcggc ttatttgttg aagtgttctt cttaggaatg
     1141 ccgctttccg ctccgccaat tagcgtgatg ctcgtgattt tggctgtagt tacttgtgcc
     1201 tccattctag aagccgctgg cggtttgaaa tttatgttgc aagttgccga acgcattttg
     1261 cgtagcaacc caaaacgtat tacctttctt ggcccacttg tcacttatct gatgacctta
     1321 atgctcggca cagggcattc ggtttactct attatgccga ttatcggtga tattgcctta
     1381 aaaaataaaa ttcgcccgga acgcccgatg gcagcagcct ctgttgcttc acaattaggt
     1441 attaccggta gcccactttc tgctgcagtt gcctattact taaccgaaat cgttaaatta
     1501 ccgggctttg aaggggtgac tttattgaat gtagtcggcg taaccatcac cgcaaccttc
     1561 tgtggtgtga ttgcgatgtc attatatagc ttacgtcgtg gtaaagagtt ggaagacgat
     1621 cctgagtatc aacgccgtat gcaagaccct gagctacgca aacaaatcga gcaaacgagt
     1681 gcaacgtctt tagacgaaaa actaccggca agtgcgaaaa actcggttta tctgttccta
     1741 gccgctattg caaccattgt gattattgca atgattccgg ctattaaacc aacggttgct
     1801 gctacaggaa aagttgccgg tatggatcaa atcattcaaa tcgtgatgct aactttcggg
     1861 ggtttaatct taattttaac taaaaccgac ccgaaaaaag taccaaacgg catcgttttc
     1921 aaatccggta tggtagcaac cattgcgatt ttcgggattg cttggatgag tgatacttac
     1981 ttcacccatg caatgccagc gttcaaagaa ggggtaacag aaatggttcg agcccaacct
     2041 tggacattcg ccttcgcctt gtttgcggta tctgtagtag taaacagcca agccgtaacc
     2101 gcaaaaatgt tattaccggt aggcattgca atgggcttgc ctgctccatt attagttggt
     2161 ttaatgccgg caacttatgc ttacttcttt attccgaatt atccgtctga tatcgcaacc
     2221 gttaactttg acgtaaccgg tacaactaaa atcggtaaat attacttcaa ccacagcttt
     2281 atggctccgg gtttagttgg cgtaattact gcctgtattg tcggtatcgc cgttgcaaat
     2341 gtgctaattt aattttaaca aaaaaacaag cggtcagatt aagtaaattt tttaccaaat
     2401 ctgaccgctt gattatttac tgcaaaacca attaggcttt gttaaagagt tttgcatcta
     2461 acgagtattt tccgccaccg ataaaaatat aggttaagaa taataccgaa tacacgcctg
     2521 ctaattcacc gccgtttaat aaaggtaata gcacattgcc ttgagctccg tgagcaataa
     2581 agaacgcgta tgccatcata cctgaaagga taaacgctgt ttggcgggta aatagaccta
     2641 aaataaagaa aattccgccc acaatttcca aaataccgcc tacaccataa atggaggcaa
     2701 ggggtacact gccgtttccg ttggtcatcg acattggaaa ttcaaaaaat ttcgctgtgc
     2761 cgtgtaataa aaacatataa ccggcaacaa cacgggtgat taaaaacgct aaaggttgta
     2821 aatttgttaa agtagagttc atttgctttc cttaaaataa attgtgatat aaaggtcaat
     2881 tgattgaggc aggcattcta ttttaaaaac ctatacttca caatccagcc gtcatgaaat
     2941 agattattta ccattaataa acaattaggc tctcgctaac acccaaacct gaatttctac
     3001 tactccagct ttgagtaact cggcacaaat tacatttaag gtggaacctg tggtgagcac
     3061 atcatcaaca atcgccactc gtttataggc ttttaacggt tgatagaaaa atgccccacg
     3121 cagatttttc cgccgttcag cagcggtcaa ttctctttgt gagacggttg ctctcacacg
     3181 ttgtaaactc tgggtatcca gcggaatctg taaccacttc gataaccaat cacacaataa
     3241 ttcgctttga ttaaaccctc ttttccaatg tctgtgccaa aaaagtggta caggtataat
     3301 cacttcaggc aagctcagaa cctgttccct ttgagcttgt ttgattgcca gcagcaattg
     3361 tcgggcaagg gcttgatccc accaatattg ctgctggaac ttaaattgat gtacccaatg
     3421 tgaaagtggc gctcggtatt catttaccct cactaattta tgccacttcg gttcattttt
     3481 caaacaatag ccacaagcgt ttcgaaactc caccgcactc attccacaat gacgacaata
     3541 aggggttggt tttagcaatt tttggcaatg gctgcaaatg ccttgcacgc caatagcgag
     3601 tggatgatta caatgaaagc aacggaagcc gaataaattc acatttcctc cttttatttg
     3661 cagattatca aatttagcct tattttttcc ggctattttt gtgacatcgc tcgaaaaaag
     3721 agtaaaatct cattatcagt aaataaaaag gaaaccgaga tgaaatccct ccaaaaaccg
     3781 atcaaaattg tctttttcga tattgatgac accctctatg taaaacataa agcctatatt
     3841 ccaaaatcaa ttaccgagca ggtattacct cgtttaaaag ccaaaggcat tattccggcc
     3901 attgccaccg gcagaaatca cggctgtttt cctgaagctc tcaaacctta tttaaaccct
     3961 gaaatcggtt ttgagctgtt tgttaccatt aacgggcaat ataatttcta taaagatcaa
     4021 ataattagtg attattcgtt aagccgtgag caaattgaac gtattatcga aaaagcgaat
     4081 gctttgggta ttctgtatgc ctttattacg ccaatgacga ttgcagtttc cgaaaccaat
     4141 ccgattgtgc acgaagcggt tttaccgatc acaccgaatt atttgatcga tccacactac
     4201 tacgaaaaag agaaaattgt gcaaatgtta ctgttctaca ccgaagagca gaaacaagcg
     4261 gtcgaaaata gtggcatttt tgcaaatgat ttaaaagagg ctcgctggca ccccaatgcg
     4321 gtagatattc tgcgaaaaga aaattcgaaa gctcgtggca ttcaagatgt attagcaacc
     4381 ttgaatattg agattgaaag tgcgatggca tttggcgatg gtattaacga cttggaaatg
     4441 ctcccgaccg taggtgttgg tgtggcaatg ggtaatggcg tgccggaatt aaaagcggtg
     4501 gcagatttca tcaccaagcc gcttgaggaa gacggcattc tatacgctct tgaaactctt
     4561 gaggtgattt aaaagcaagc ggttgtaagt ttttctccat ttacaaccgc ttgtagtttc
     4621 taattacgcc taaatagctt atcgcctgca atcgctaaaa tcgccccact gacgaccacc
     4681 aacgccccaa gataactgat ccaattcata tccggcttgg caaaaatatg cggataaaaa
     4741 tagtagctaa ggttggaaaa gatcatcgta aaaatcggca acatcgtggt tacaatgcta
     4801 acctttgaag tatcccaatg attcaacgcc tcaccgtatg aaccgtagcc aattaaggtg
     4861 ttcaacagca ataaataaag caaccccaca gaaaaccatc ctcaagattg cctaactgtg
     4921 ccggtgaggc aaacggtaac atcagcagag aacagccgat gtaaataata aacagcactt
     4981 gctgagagtt aaatttcgct aataataatt tttgagccac gccgtatagc acccaaatca
     5041 agctggctcc ggcactcaat aacactccga gagcgtaggt gttgagctgt aaaatttcag
     5101 caaatttatc gttaaaaaat gcgacaagcc ctgcaatcaa taacacaaat ccaatctttt
     5161 gaaacaagcc gaacttttcc ttaaacaata atacgcctag tagaatcatc gaaaatggag
     5221 ctaactgcca caatacttgg ttaatggtgg gcgaaacata gtgtagagcc tctgcaaaca
     5281 aaaagaaatt tgccgacaga ccacacaccc ctaacaaaat taaaccgagt tcttttttgc
     5341 tacaagcggt caatttgggt aaacgttttg caaatcctag aatcagcaat agccctactg
     5401 atgccacaac gaacctaacc caaactaaag tttgggcatc tatcactttc aatacttgct
     5461 gggcggcaat cggtagcgtt ccccaggtca ttgctgcggt taatgcaagt gaaaagccaa
     5521 gtaaggggcg ttgtttcatc gtggaaattc ctttttctat tctatgtttc tatattttag
     5581 aaagattgag atacaaaaaa accccgccat tggcgaggtc tcttattgat tttgaaatga
     5641 ttagtcagca atgatgctta catatttacg gcttttctcg cctttcactt caaatttaac
     5701 tttaccgtca gcagttgcaa ataaagtgtg gtctttaccc atacctacat ttgaacctgc
     5761 gtggaattta gtaccacgtt gacgaacgat aatgctacct gctaatacag attcaccacc
     5821 gaaacgttta acaccaaggc gtttagcttc agaatcacga ccgttacgag ttgaaccacc
     5881 agctttttta gttgccatct tctagatctc ctctgaaatt atgcttgaat cccagtgatt
     5941 ttcacttctg tgaaccactg acgatgacct tgttgtttac ggctgtgttt acgacgacgg
     6001 aacttaacga ttttaacttt ctcgccacga ccttgtgcta caacttcagc cactactttt
     6061 gcacctgcta caaccggtgc accgatttta acatcttcac cgttaacgac cattaacact
     6121 gagtcaaatt caactttctc accagttgca agttcaagtt tttctaaacg aactacttga
     6181 ccttcactta ctcggtgttg tttgccgcca ctttggaaaa ctgcgtacat atttactccg
     6241 ctatgtgcca actgcttttg cttttaggca ctaaattaat tcataaaaat agggttgcaa
     6301 ttctacggaa aaagattcga aagagcaaga gcgattttga gaattttttg aaatttgcaa
     6361 aaaatggtaa aaatactacc gtttgtttca actgcgacta aaaaacaata gactgctccg
     6421 attttacaac aaaatcataa ttcttttttg aacacaggct aaattaaggt agaatctact
     6481 caattttcgt cttactttat ggattcgatg gctgtgcaac ttttttcttt agaacaaatt
     6541 caagcccttg cgaatgaaga tatgcaagcg gtaaatgccg aaattttagc tcaacttaat
     6601 tccgaagtgc tactgattaa ccagctcgga cattatatca tcgcaggcgg tgggaaacgt
     6661 attcgcccgt taattgcggt gcttgcggct cgtgccttgg gttatcgtgg taaaaagcat
     6721 atcacggcgg ctgcttttat tgagtttgtg catactgcta cacttttgca cgatgatgta
     6781 gtcgatgaat ccgatatgcg tcgtggcaga ccaaccgcaa acgcccattt tggcaatgcg
     6841 gctagtgtat tggtagggga tttcatctat acccgttcgt tccagatgat gacggattta
     6901 gactctctgc gtgtgttaaa agtgatgtct gccgccacca atgcgttggc agaaggggaa
     6961 gttcagcaat taatgaacgt gaacgaccca aacaccactg aagcgaacta tatgcgggtc
     7021 atttacaata aaaccgcccg cttgtttgaa gccgccactc aatgcgtggc gattgtggct
     7081 gatgcttctg aggaaattga aaccgcatta aaaaattacg gttgctactt aggcacggca
     7141 ttccagctga ttgacgatat tttagactac tccgctcaaa gcgaaagttt aggcaaaaac
     7201 gtaggcgatg actttgtgga aggtaagcca accttgccat tgctccacgc aatgcacgct
     7261 gccgacaaag caggcaaaac tactcaagca gagctgattc gcaatgctat cgaaaacggc
     7321 ggcgaattgg aacaaattcc acaagtattg gcaattatgc aagaacatca atctctggat
     7381 tatgccatgc aaaaagcccg tgaagaagcc caaaaagcgg tggattcatt aggggtttta
     7441 ccggaaagcg agtacaaaca agccttggtt tcattggcgt atttatcggt ggatcgctct
     7501 tattaatttg caaaaaatca tataaattta accacttgta ggggcaaatg atatttgccc
     7561 cataacctat aattcttttg ggcaaatgtt atttgcccca acataacaat gactgaacaa
     7621 aaccaacaaa accaacaacc ccaagaacat attgaaagcg tacgagcgag acgccgtgct
     7681 caaaatcaga aaaaagaccc gaatgccccg tttgtgcgtg agaaattaac gctgccaaac
     7741 gggcataaca aattactact gcactcttgc tgtgcccctt gctcgggtga ggtaatggag
     7801 gctattcacg cctccggcat tgagtttacg atctattttt ataacccgaa cattcacccg
     7861 ctgaaagagt atttgatccg caaggaagag aatattcgct ttgccgaaaa gtggggtatt
     7921 ccgtttatcg atttagacga tgactacgaa aacgaccgaa aagagtggtt caaaaaagcc
     7981 aaaggaatgg aatgggagcc ggaacgaggc attcgttgca caatgtgctt tgatatgcgg
     8041 tttgaaaaag cagcggaata cgctcacaac aacggtttcc ctgtatttac cagttgctta
     8101 ggcatttccc gatggaaaga tatgaaccaa atcaacgact gtggtcaccg tgcagcagcc
     8161 aaatttgatg acgtggttta ctgggactac aactggcgta aaggcggcgg ctcacaacga
     8221 atgatcgaaa tcagcaaacg tgaacgtttc taccagcaag aatactgcgg ttgcgtctat
     8281 tcgttaagag atagcaacaa atggcggttg gaaacagggc gtcagcggat tgaaatcggt
     8341 aagctctatt actcacccga ttaaatgtta cttttctttg cttcgccaaa gaaaagtaac
     8401 caaaagaaag gcggcccgaa cttcgcttag ttacgatttc cttaacggaa aatttgtaaa
     8461 ctcgctacgc tcaaacagta caaattttcc tagaaatcgc aagtcgctca gacgggacaa
     8521 gcggtacatt attcaaaatt ttttacaaaa tatagtgatg tactcttccc ttctgaaccg
     8581 cccgaacgtg tttgaattgg taggaaaatt tactctgagc gaagcgaatg taaattttcc
     8641 gtgaaaccaa ttcaaactaa gtgaggataa gcggtgaagt aggggtgttt ctttggttac
     8701 tttctttgca caagcaaaga aagtaacgaa aactcagata tagatttcaa cttaaaaatt
     8761 tacagaagga ataataacaa atgtcaaaat tcgctatggt tttccccggt caaggctcac
     8821 aagcggttgg aatgcttgcc gaccttgcgc cacaatatcc aatcgtggaa gaaaccttca
     8881 aacaagcaag tgatgtgctt ggctatgact tatgggattt agtgcaaaac ggtactgccg
     8941 aagagttagg gcaaacccac cgcactcaac ctgcgttgct tgcctcaagc gtggcacttt
     9001 tccgcatttg gcaacaaaaa ttccctgagc aaaaaccagc cgtgatggca gggcatagtt
     9061 tgggtgaata ctcagcgttg gtgtgtgccg gtgtgttgga tttccaagat gcgattaaat
     9121 tggtcgagtt gcgtggtcgt gcaatgcaag aagccgtgcc ggcaggcagc ggtgcgatgt
     9181 ttgcgattat cggtttagat aacgaaagca ttattaaagc gtgcgagcaa gcagcaagcg
     9241 agaccggtga aattgtctct gcggtaaact ttaacagccc ggggcaagtt gtgattgccg
     9301 gcacaaaagc agcggcagaa aaagcaggag aattgtgtaa agccgcaggt gcaaaacgcg
     9361 cgttaccatt agcggtgagc gtaccttcac actgtgcttt aatgaaaccg gcggcagaaa
     9421 aattagccga agccttgcaa aatattgagc taaatcaacc gcttgtccct gttatcaaca
     9481 acgtggatgt ggcagtggaa acagatgccg ataccattcg taatgcactt gttcgccaac
     9541 tttacagccc tgttcgttgg acggaaaccg ttgaaaaaat ggcacaagat ggcatcacta
     9601 ctttatatga aatcgggcca aacaaagtcc taaccggttt agcaggtcgt attgtaaaag
     9661 aattatcagc caaagcggta aatgatgttg ccagctttga ggctgttgag gcttaatttt
     9721 cgggagcgta aaatgagtaa agaaacccta tttatgctaa taagtgcatt gattgcctta
     9781 gtgattttac ctttagctta tactgttggt atcgctaaag aaacattagg cggaaatatg
     9841 ggaatgttat ccattgctgc cggttgctca tttttagcct attttttaag tctaaacccg
     9901 aaaattcgcc acgcctttat cggaggttca gctattacag ctgctgctat tcaagcttgg
     9961 gtattatatg gtgtgctatt aagcgaaaat gaccccaaaa gtgcggtcgt tttatttcaa
    10021 atttatctgt ttagtagttt agggccactg tttttttcag ctatttgggc atttactcgc
    10081 ccacgttgga tttaccctaa acaaggtagt gcggtgaaat atgccttatt tggtggctta
    10141 acgaccgtga taaactactt gattaccctt tttatttttt atattctgac gttataacgt
    10201 catactaagg agaatttatg agcaaaatcg cattagttac aggggcaacc cgtggaatcg
    10261 gcaaagcgat tgccgaagaa ttggtagcaa aaggctacat cgtagtcggc accgcaacct
    10321 ctgaaaaagg ggcggaatct atttcagctt atttaggcga gaacggcaaa ggcttagtgc
    10381 taaatgttgc cgatgcagag tcgattgaga gcgtattaaa gcagatcaaa gagacctttg
    10441 gcgatgtgga cgtgttagtc aataacgccg gcattacccg tgacggctta ttaatgcgta
    10501 tgaaagatga ggattggttc gacatcattc aaactaactt aacttcggtt tatcgtttat
    10561 ccaaagcggt attacgcccg atgatgaaaa aaggcggacg cattatcacg atcggttctg
    10621 tggtcggctc aatgggcaac tcggggcaaa ccaactactg tgctgccaaa gcaggcttag
    10681 ttggtttttc taaatcttta gcgaaagaag tagcaagccg cggcattacg gtaaacgtgg
    10741 ttgctccggg ctttatcgcc actgatatga ccgatgagct aaccgaagat cagaaaaatg
    10801 cgattttaag ccaaattcct gccggtgaac tcggttctcc acaagatatt gccaaagcag
    10861 tcgctttctt agccagcgat gatgcccgtt atatcaacgg tgaaaccatt cacgttaatg
    10921 gcggtttata tatgagctaa tccgctacaa gcggtctttt ttcaaccgag ctttgtaaat
    10981 tcagagttcg gttttgttat ttgttatttt gtgttatcac ctgctttata ggtgaaaaag
    11041 tgtgaatatg ttatgactag aaatacatta gtagtaggct ttttattgtt tgctattttc
    11101 tttggtgccg gcaatctgat tttcccacca aaattaggtt atgaaagtag tacagaattt
    11161 ttctcagcca tttcaggctt tgttttaact ggagtagggc ttcctctact cggtattatc
    11221 gtcagtgcat tttataatgg aggctataaa tctgcattag acaaaatcca cccttggttt
    11281 tctttagcat tcctaacttc tatctattta gcgatcggtc cattctttgc cggtccgcgc
    11341 acggcagcaa ctgcctatga aatggcgata atgccgtttt taggggattc cagccaagtg
    11401 tcaatgttaa tttttacctt aatttacttt gcctttaccc tatggttagc gctaaatcca
    11461 aataaaatgg ttgatcgcat tggtgcaatt cttaccccga tcttgcttat aaccatcatt
    11521 ggattagtta ttcgagccgg ctttatttta gataccgatc cacaaatcac tacgcctaat
    11581 acgaatgaac aatcttactt ttttaaaggt atggttgatg gctatctaac tatggatgca
    11641 cttgcttcgt tagcctattc ggtaattgta ttaaatgcta ttcgtgggaa aggggtaagc
    11701 caagccgcat tacaacgcca aacagtttct gccggcttag ttgcagcagt tgctctagct
    11761 gtaatttata tttcattggg ttggattggt aataatatgc caattagtgc agacacactt
    11821 tcgatgttat cagaaagaaa attggatcta ggtacttaca ttcttaacac tgtgactacc
    11881 caagccttcg gtgaactcgg acgtagtgtc ttaggaatga ttgtttcact tgcctgttta
    11941 accactgctg tcggtttagc cgtatcagtc agtgaatact ttaatgaaat tttcccgaaa
    12001 atttcatata aagtttatgc tatcttattt actgtgatta gttttactgt tgctaaccaa
    12061 ggattaaaag aagttattag caaatccatt ccggtattat tagttctcta cccaatagca
    12121 atgactgtca ttttaatgtt gctgattaat ttagtgatta aacttccgtt gtctgcacaa
    12181 cgtctatcac tcctattagt gacaattgta gcgatagctt cagttgccgg tgtcgggttt
    12241 accgaaaact tgcctctaaa atcctactcg atggaatggc tacctttcgc tatcattgga
    12301 gctattattg gagcaattat ccacccgctt accggtcgaa agcataaata aagtaaaggc
    12361 agtgagcatt cactgcctct tttttaggag agaataatgc ctgaattacc cgaagttgaa
    12421 accagcgttc gtggtgtatc gccctatttg gttgggcaaa ctattgaaca aatcatcgtt
    12481 cgtcagccaa agttacgttg ggcggtgagt cctgaattaa gccaaatggc aggggcaaaa
    12541 attaccgcag tagaaagacg tgccaaatat ctgattatcc gcaccgacaa aggcgatatt
    12601 ttggtgcatc taggaatgtc cggctcgctc aggattttgt ctcaaaccgc cgatatcgct
    12661 ttcggtaagc acgaccatat cgatttagtc accaccaatg gcacaatttt acgctacaac
    12721 gaccctcgca aattcggttg ttggctatgg gcagaaaaag cagaagatca cgaactgttg
    12781 cgtaaactcg ggcctgaacc gctttcaaac gatttcacag cagattactt atttgcaaaa
    12841 agccgcaaaa aaacgaccgc ttgtaaaacc tttattatga ctaatgaagt ggttgttggg
    12901 gtggggaata tctatgcgtg cgaaagttta tttatggcag gcattcaccc tgagcttgca
    12961 gcacaaaacc tgactaaaaa acagtgcgaa cgtttagtaa aaaccatcaa agaagtacta
    13021 acgaaagcga ttattcaagg cggcacaaca ctgaaagatt ttatccaacc tgatggcaaa
    13081 ccgggctatt tctctcaagt gttgcaagtg tatggaagaa aaggggaagc ctgtaatgac
    13141 tgcggaaaga caattgaaac caaagtaatc gggcagcgta atacctattt ttgcccgaag
    13201 tgtcagagat tgccgaaata atctaataat aaactctctc cccctgaggg agagagacag
    13261 cttgttgaaa cgttaagtga aacaagcaga gagagggtaa aacctaaacc tgcaaccaaa
    13321 aggtaacagg cccgtcattt tgtaggctga cttgcatatc ggctgcaaat tgtccggttt
    13381 gggtgtgaat tttagtggcg gcttgttgat gaaagtaagc atacaaacgt tcggcatcaa
    13441 ccgggcttgc cccttttgag aaactaggac gcaagccttt attggtatcc gccgcaaggg
    13501 taaactgtga aaccactaat aagctgccac cggcttgctg tacgtttaaa ttcattttcc
    13561 cctcgctatc cgaaaacacc cgataattca gcactttttc cagtagctta tctgctttcg
    13621 cttcatcatc gtctttttcc acaccgagca acaccaacaa accgcttgca atttcgccaa
    13681 cgacttgccc ttcaacctcc acttttgcga atttaacccg ctgaattaag cctatcatta
    13741 acgctcccct tatctgtttc catttcgtct aaaaccgccg caaattgtcc gccaagtaat
    13801 accacctgcc aacttaactg aatccataac agcataatcg gtaacaccgc taatgctcca
    13861 taaattgcct gataagacgg aaaagcagta atataccaaa taaaggcttt cttccctaag
    13921 gtaaagaaaa tcgctgccat taatgccccg atagcagcgt gtataaattt tacctttcta
    13981 atcggaataa atttatacac taaggtaaaa agtaaccaaa ttaataaaaa cggcgtcaat
    14041 tctaataaat gctgcccgag cgataacgcc ccacttccat taaaaataga aagcgacatc
    14101 acataggtgc taattgcaat actcgcgccg gcaagcagcg gcccaaacag caaaatgaga
    14161 aaataaagca aaaaagagaa ccacatcggg cgctgacgat tatcgtgcca aatggtattc
    14221 aatgcttcat caatactctt aatcagcata atagtaacca caaataagcc gataaaactc
    14281 accagcccca tttgtttagc attactgaca aacaactcaa tgtattccga caccatctcg
    14341 ctcgcactcg gggcaaagtt gtcataaatg aacgctttta actgctcgtt tgcttcgtgg
    14401 aaaatcggaa aaaaggtaaa aatggaaaac agcacgacaa taaacggcac tatcgccagc
    14461 attgtgctat aagtgagata accggctgaa atcgcaatgt tattttcatt gtaacgcttg
    14521 gcaaagagct taataaatct ccacagtcga tttaacatct taacctctca ccaaataatc
    14581 gccttcacac acccatttgt aggtggttaa ggcttctaat cccatcgggc ctcgtgcgtg
    14641 taatttctgc gtgctaactg ccacttctgc ccctaaacca aactcaccac catcggtaaa
    14701 acgggtgctg gcattaatat aaaccgccgt ggcatcaacc tgttgtacaa attgacgggc
    14761 aagttgataa tcggaggtta aaatcccctc tgaatgctgc gaaccataat ggcggatatg
    14821 ctctaccgct tgttgcaggt cattcaccac caccacgtta agatcaagcg ataaccactc
    14881 ttggctcaat tttgcttgat caagcggttc aattccttca attttttgca aatcgttact
    14941 gtgaatagta acgccaagct gttgcatttt ggcgaccaat ttcggcaaaa attccaccgc
    15001 aatcgctttt tccaccaaca gcgtttccag cgtattgcaa gtgcttgggc gttgcgtttt
    15061 ggcattagcg atgacctcta gagatttctc ctgatccgct gttttttcca caaacagatg
    15121 acaaacccca atccccccca caatcaccgg aatggtcgaa ttttctttac agaattgatg
    15181 taaacctgca ccgcctcgag ggatcaccat atcaatgtag cgatctagct tcaatagttc
    15241 caataataag gctcgatccg gatccgtcac cgcttgcact gccaatttcg gcaagccggc
    15301 tttttctaac gcatcttgca ctacatcaac caacacagca ttggtaaatt tggtctcttt
    15361 accgccacgc aaaatcactg cattgccggt ttttaggcat aatgatgcca catcaatggt
    15421 cacattcggg cgagcttcgt aaatggttaa aatcaccccg agcggcacac gctggcgttc
    15481 gattttcaaa ccgctgtcta acacgccacc gtccatcacc tgccccaccg gatccgctaa
    15541 tttggcaaca tttcgcacat cgttagcaat gtcttttagc cgatcttcat tcaataataa
    15601 gcggtcaata atcgccaatg aaatcccttg tgcttgagca aattcaatat ctttggcatt
    15661 agcgtataaa atttcttctg cacgttgctc aaggctatcg gcaatcgtga gtaaggcttg
    15721 gtttttctgt aggttgccaa attgagccag ctcaaaacta gcttgtttgg cttgctgggc
    15781 aagttctatc atctgcataa attaggtctc atttaatcaa tattctccat aatctaacac
    15841 gttcttttat gtgaagaaat atgcaaaagc caagaaaatt ggataaaatg ggggaatttt
    15901 aaaacattac gcaattcccc tctccctgat ttttctcctg aacggagaaa aatctatccc
    15961 tctcctacaa ggggagaggg tagaatttaa ctctctcccc ttgtgggaga gagaccgcct
    16021 acagattcgt tcagaatctg taggcagaga gaggggtaaa aacatataaa acttaaacaa
    16081 aatgataaac actaccccta aacagctaat ccacattctt ccgcctcaac tggcaaacca
    16141 aattgctgcc ggcgaggtgg tggaacgccc tgcgtctgtg gtaaaggagc tagtggaaaa
    16201 cagtttagat gccggagcca atactattca aatcgacatt gaaaaaggcg gctctcagct
    16261 tatccgcatt cgagataacg gttgtggtat cagcaagcaa gatttaccgc tggcacttgc
    16321 tcgccacgct acgagcaaaa tcagcagctt ggaagattta gaagccattt taagtttagg
    16381 ctttcggggc gaagcattgg caagtatcag ctcggtttca cgtttaacac tgacctctcg
    16441 taccgcagag caaaccgaag catggcaagc ctatgcacaa gggcgggaaa tggcggtgga
    16501 aattcagcct gcctcacacc cgattggcac aaccattgaa gtagcaaatc tgttttttaa
    16561 cacaccggca cggcgtaaat ttctacgcac cgataaaacc gaatttgccc atattgacga
    16621 ggtggttcgc cgcattgccc ttgccaagcc gaatatcagt ttcagcttaa gccataatgg
    16681 taaaatcgtc cgccaatatc gcaaagtgca agacaattcg gtagagcagc aacaaaaacg
    16741 ggttgcagcc atttgcggtg atgagtttat tcaacattca acttatattg actggcaaca
    16801 cggggatctg cacctgcacg gctgggttgg ctcaccggct cttgcccgtc tgcaaaacga
    16861 cctttgctac agctatgtga acgggcgaat gatgcgggat aaaaccatca atcacgccat
    16921 tcgccaagcc tacggcgaca gcattgcaag tggttactat ccggcttttg tgatcttttt
    16981 ggatttagat ccgacaatgg ttgatgtaaa cgttcacccg gctaaacacg aggtgcgctt
    17041 tcatcaagga agactggtac acgactttat tttgcaaggc gtactcaaca gcctgcaaca
    17101 aacccaacct acgcttgaac tgcgaaatga agtgaacgag ccgttaccga gctatattca
    17161 agacactaac cgccaagctg cggggcaaaa tatttttgtg tcgcaaaccg ctccacaagc
    17221 ggccgaattt acgcaaaaaa atgcaagtcg atcaagctat acgcctaaat ttgaacgaag
    17281 caacagtgtg agttcgtccg ctcaaaaatg gtataaggaa ttggttggtg gcgatttgca
    17341 ccaaacaacg ccaactatca aaacgcaaag cacgcttgtt gagccaaaaa cagaacccca
    17401 aactgaacat aaaacagaag ccgtttcgca aaaaattacg caaattgcac cgcttgcaag
    17461 ccaccattca caagccttag cgattgtaaa aaaccaagcg gtgttaatta aagaagagga
    17521 aaatttttac ctgcttccac tggcaacatt agccgagctt aaactcaagc tacaactgga
    17581 aaaagggcaa tctcaagcgt tgttgatccc actgaatctc agtttagatc aacaacaaac
    17641 cgaacgctgg caacaaacca aagaagaact aactgagctt ggcttttcga tcagcgaaaa
    17701 acagtggcaa gcccaaacac ggctcacagt gctttccgta ccacaggctg tcagagaaca
    17761 aaacctgcaa cagcttttat ttgctctgtt caaccaaaca caagcggtca atttagccga
    17821 attttttgca aaaaaatgcg aaaaacccac cgcttgtacg ctaagtgacg tgattgctct
    17881 actctcagaa attgagcttt atgccaatgg gaaaaaagag ctggagggaa taaggaagga
    17941 agtggatttt tccaagtttt tatagtctaa agggcaatac cctttagcca aataaatgag
    18001 agtggctgcc aattcgcagc agctctagct tatcatcttc cacttttcga tagataagaa
    18061 ccaaatcagg ttggatatgg caatccctaa aacctttcca ttcgccggtt aaagcgtgat
    18121 cgcagtattt ctcaggtaat ggcttgtcat taatcagaaa atacaacgcc tcaattaatg
    18181 aagaggataa agactcaacc gcacattttt tatagtcacg tttaaaagca ttactatact
    18241 caatctctcg cattgagttg ctccattaaa tccgcaatac tatttgctga atgtagcttt
    18301 tcctcgccat tcttaacagc ccttaatact tctgctgtct caaaattagg ctgataatca
    18361 aactgaaaac gtttttctgt tgcgattttc gctaatgtca ttcgcatcac atcagagaca
    18421 gtcaagccga taccggctaa cacctcactt gcttgctctt taatttcagg gctgattctt
    18481 acgcttacta atgcactatt tgccatcaaa aactcctttt gtatatcaaa tgatatacaa
    18541 aataacattt aacaaaaaat tgtgcaataa aaaagaaatt caaagaaaag aaatgaatag
    18601 ttaaaattag ctcgttacaa gaaatcattg agttactcaa gaaatcaagg taatataaag
    18661 aagtctgggc ggaacacgaa cccgccccta gcatctagcc gcttacaaga acagatagat
    18721 tagttgaaag agcgtgaaaa tttcaactgc tgcacacaga aacccaagtg caactaaaat
    18781 caatagtagt tctttcattg taagcctcct tagtgctact aaggatgatt taggctcagg
    18841 ctactgcgaa tagccctaac gagcctacca gtagcagtag cacaatcagg attgtaacaa
    18901 aaaagctgtg aatttcacgg ctttttttgt atctatatac agtatattaa tatgaataaa
    18961 aaaccccttg cccttttttt aatggggccg actgcctccg ggaaaaccga tttagctatt
    19021 gctttacgcc aagaattgcc gattgaagta atcagtgtcg attctgcctt aatttacaaa
    19081 ggaatggata ttggcacggc aaagccaagc aaggaagagc ttgcccttgc accgcatcgc
    19141 ttgattgata ttttagaccc tgccgagagc tattcggcga tgaatttcca cgctgatgcg
    19201 ttgcgtgaaa tggcagatat tacctcacag ggtaaaattc cgctcttggt gggtggcaca
    19261 atgctttatt acaaggcatt gcttgaaggg ttatctcccc tgccctctgc cgatccagcc
    19321 attcgggctg aaatcgaaag ccgagcggca caacacggct gggcaacatt gcatcaagaa
    19381 ttagtgaaaa tcgacccgat tgccggtgca agaattaacc caaatgacag ccaacggatc
    19441 aaccgagcgt tagaagtttt ttacattacc ggcaaaacga tgaccggact tactgctcag
    19501 caaggtgagg ctctacctta taatatctta caatttgcaa tcgctcctca agatcgtgca
    19561 gttctacacc aacggattga acaacgcttt cacaaaatga ttgagctagg ctttgaagag
    19621 gaggtcaagc ggttattttt acgcaaagat ttgcatatta atttgccctc gattcgctgt
    19681 gtgggctacc gccaaatgtg ggaatatttg caaggcgata tttcgctcga tgaggcgatc
    19741 tttaaaggca tttgtgccac ccgccagctg gcaaaacggc agattacttg gctgcgtggc
    19801 tggcaaggcg aactgacttg gttagatagt ttagctccga ccagttctaa agcaaaaatg
    19861 gtggaaaaga tcgatcatta tttaaagaat tgtgataata tctaaacgcc tttggcactt
    19921 atcgcattat ttggtgtctt aaaaatcaca caactataaa aaggaaaata acaatggcaa
    19981 aaggtcaatc tttacaagat ccatatttga atgcacttcg tcgtgagcgt attcctgttt
    20041 caatttattt agttaacggc attaaattac aaggtcagat cgagtcgttc gaccaatttg
    20101 tgattttatt aaaaaacact gtaagccaaa tggtgtataa acacgctatt tcaacggttg
    20161 ttcctgcccg tgcgttatcg cataacaata acaaccaagc ccaacacgct cagcctgctg
    20221 ccgaagtaac tgaacaagcg gaataataac attattactc aaccaatgtt aaacgaatca
    20281 ttgccaaact cagaagaaaa ttctgcgttt ggctttgctt tatctgaagc aagccaaact
    20341 acagaagccg cctcgcctac cgatccagaa aatcaagata aagccatttt ggtgcatctc
    20401 tatctcgggc aacataaaga tgttgaaaat ttgctggaat ttaaaacgct ggcagaatct
    20461 gccggtgtcg aagtgctggc aacgcttacc accagccgcc aaagtccgca tatcaaatat
    20521 tatgtggggc aaggcaaagc ggaggaaatt gccgaagcgg tagaacaact cggggcaaca
    20581 gtagtgctgg tgaaccatca acttagcccg gctcagaccc gtaatttgca atcgttatgc
    20641 ggctgccgtg tggtggatag aaccggcttg attttggata ttttcgctca acgggcaaga
    20701 tcgcacgaag gcaaactaca agtcgagctg gcacaacttc gccatttatc gacccgtttg
    20761 gttcgccgag ttactaatca agaccaacaa aaaggcggag cagttggctt gcgtggtcca
    20821 ggtgaaacac agctcgaaac cgatcgccgt ttgattaaag tgcgaatcca acagttactc
    20881 aaccgcttgg aaaaagtcag caagcaacgc aatcaaaatc gacaaaccag acaaaaagcc
    20941 gatattccaa cagtatcctt agtgggctat accaatgccg gcaaatctac cctgtttaat
    21001 gcgattaccc aagctggtgt ttatgctgcc gatcaacttt ttgcgaccct tgacccgact
    21061 ttacgccgga ttcaggtgca agatgtcggc acgacaattc ttgccgatac ggtaggtttt
    21121 atccgttttc taccgcacga tttagtttca gcctttaaat ctactctgca agaaaccacc
    21181 gaagctactt tactgctcca cgtcattgac ggctcagacg aacgcaaaaa cgaaaatatt
    21241 gatgcggtaa atcaagtgct ggacgaaatc ggagcgttag atattcctac tttattagta
    21301 ttcaataaaa tcgataaatt agaggatgta gaaccgcata ttgaacgcaa tgctgatgct
    21361 atgccagttg cggtgtatct ttccgcccaa gaaaatagcg gcattgagct gttattccaa
    21421 gccattcaag aaaggcttcg cagtaactta gtgtgtgaaa acttgctcct cccggttact
    21481 gccggtgcaa tttacgcaca atttaaaaca gaaaactgca ttaaaaccga gcattttaat
    21541 gagttcggtg aacggatgat ctatattgaa attaacgaag tgcaatggaa caaatgggtg
    21601 aaacaatttc cggatctagc cgaatatatt gaactggcaa aatgggggta attaatctca
    21661 cttctgagta agttatattt aaattatgca agcggtcaat tctttacgtt tttttgcaaa
    21721 aaagtgtaaa gaattgaccg cttgttttat ccattaaatc caatttagcc aataaaaaat
    21781 ccctcaaata aaccagattt ccccgttcta tttgaaggat tttatttatt cttccccaaa
    21841 agtggggaag aaaagcttaa tcaagcagtt actaccactg ata
//
LOCUS       DANJWC010000022        21068 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.022, whole genome shotgun sequence.
ACCESSION   DANJWC010000022 DANJWC010000000
VERSION     DANJWC010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 21068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21068
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.022"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(204..1553)
                     /locus_tag="PWX02_000623"
     CDS             complement(204..1553)
                     /locus_tag="PWX02_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDL1728661.1"
                     /translation="MLLFIMFAAIILLLVLIMKFKVHAFVALIIVSLLTALAAGIPVD
                     KILPTLLNGFGSTLASVALLVGLGAMIGRLLEITGGAKVLADTLINKFGKEKAPFTLG
                     VASLLFGFPIFFDAGLVVMLPIIFSVAKQFGGSVLRYAFPSAGAFAVMHAFLPPHPGP
                     VASGDLLGVNMGLLVLVGLVCAIPTWYIGTYLFSQFISKRIHVDLPKAFLNGLSANEM
                     AVQNPPSFGKVLTVLVLPIILILFDTGLNTLSVAKVIDGSEMWVQSLRLIGKTPVALL
                     ITLIVAIMLLRDNRSFDQIEKICNNALGPICSIVLVTGAGGMFGGVLRASGIGDVLSE
                     MMKDTGMPIVVAAFIIATVFRVVQGSATVALTTTAALIAPTVAAATDLSQFDLCFIVI
                     AIASGATVLSHVNDSGFWLMSRFLEMDEKTTLKTWTMLETSIGLTGFACALIGSWLL"
     gene            1740..2255
                     /locus_tag="PWX02_000624"
     CDS             1740..2255
                     /locus_tag="PWX02_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDL1728662.1"
                     /translation="MANGKAFILMGVSSTGKTSVGTAVAQRLGIKLIDGDDLHPRANI
                     LKMASGTPLNDADRTPWLERINDAVFSFEQKSEKGIIVCSALKKKYRDQIRQGNADVT
                     FLFLHGSFDLVLARMQQRKGHFMKPEMLQSQFNTLEIPQADEPDVIFIDIDGSFDEVV
                     ERCIKAAEPLI"
     gene            complement(2309..3307)
                     /gene="gntR"
                     /locus_tag="PWX02_000625"
     CDS             complement(2309..3307)
                     /gene="gntR"
                     /locus_tag="PWX02_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HDL1728663.1"
                     /translation="MSKPKRPTLQDIASHLGITKMTVSRFLRDPNTVAKETGERIAQA
                     IELFGYIPNRAPDILSNAKSRAIGVLVPSLTNQVFADVIKGIEQITDQAGYQTMLAHY
                     GYSEQKEEQRIESLLSYNIDGIILSENHHSPRTLKMLEVANIPVIEIMESGECGIQQA
                     VGFDNISAAQAMVETMIHRGCRQVVYFGARMDKRSQLKMQGYEQAMLKHGLSPHSITT
                     AEHSSFTLGASQLKQALEQIPQLDGIFCTNDDLAIGALFECQRLGINVPQQIKIAGFH
                     GHDVGQSLTPQLASVMTPRLEMGRVAAQELLDRIHGVPQQSPIINLGYHIHLGESV"
     gene            complement(3400..4560)
                     /locus_tag="PWX02_000626"
     CDS             complement(3400..4560)
                     /locus_tag="PWX02_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDL1728664.1"
                     /translation="MIEQSYKIALLFNANKIYDRGVMQGIGQFIQASQCTWDIFVEDD
                     FVYHKNSIESLLIDGIIADFDDLETVELLKDIDVPIVAVGGSYQNSDYYPNLPYVATD
                     NYALIEAAFLHLKQKGIDQFAFYGYPAETEKHWAIERQNAFIQLMEKYEHQPNCYIGD
                     QFHSDNWVELQAKLGEWLQTLPPHTGIIAVTDARARHLLQTCEHLKIAVPEQLCVIGI
                     DNEELIQYLSRVSLSSVTQGTSQIGYHAAKLLYRKLNNLPVSLKPILIPPVKVEERRS
                     TDYRSLQDPMVIQAMHFIRHRACQGIKVEQVLDHLKVSRSNLEQRFKEEMNKTIHQVI
                     HEEKLARAKYMLKFTEISTQEIANVCGYPSLQYFYAVFKKEFGKTPKEFRQE"
     gene            complement(4683..5069)
                     /gene="rplQ"
                     /locus_tag="PWX02_000627"
     CDS             complement(4683..5069)
                     /gene="rplQ"
                     /locus_tag="PWX02_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDL1728665.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMASALVEHEIIKTTLPKAKEL
                     RRVVEPLITLAKADSVANRRLAFARTRNVDTVAKLFNELGPRFAQRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRPDVATEAAAE"
     gene            complement(5105..6094)
                     /gene="rpoA"
                     /locus_tag="PWX02_000628"
     CDS             complement(5105..6094)
                     /gene="rpoA"
                     /locus_tag="PWX02_000628"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDL1728666.1"
                     /translation="MQGSVTEFLKPHLVNIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSSKEGVQEDILEVLLNLKGLAVKVQNKDDIILTL
                     NKSGIGPVTAADITHDGDVEIVNPDHVICHLTDKNASINMRIRVQRGRGYVPASARLH
                     TQNDDRPIGRLLVDARFSPVDRIAYNVEAARVEQRTDLDKLVIEMETNGTLDPEEAIR
                     RAATILAEQLAAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAETIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIAED"
     gene            complement(6125..6751)
                     /gene="rpsD"
                     /locus_tag="PWX02_000629"
     CDS             complement(6125..6751)
                     /gene="rpsD"
                     /locus_tag="PWX02_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDL1728667.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIESKCRNRLDVAPGQHG
                     ARKPRLSDYGSQLREKQKVRRIYGILERQFRNYYTEANRLKGNTGENLLVLLEGRLDN
                     VVYRMGFAATRAEARQLVSHKSIVVNGRVVNIPSFQVSVDDVVAVREKSKKQARIKAS
                     LELATQREKPTWLEVDATKMEGVFKRRPERSDLSADINEHLIVELYSK"
     gene            complement(6780..7169)
                     /gene="rpsK"
                     /locus_tag="PWX02_000630"
     CDS             complement(6780..7169)
                     /gene="rpsK"
                     /locus_tag="PWX02_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDL1728668.1"
                     /translation="MAKTPVRARKRVKKQIADGVAHIHASFNNTIVTITDRQGNALAW
                     ATAGGSGFRGSRKSTPFAAQVAAERCAEMVKEFGLKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            complement(7186..7542)
                     /gene="rpsM"
                     /locus_tag="PWX02_000631"
     CDS             complement(7186..7542)
                     /gene="rpsM"
                     /locus_tag="PWX02_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDL1728669.1"
                     /translation="MARIAGINIPDQKHTVIALTAIYGIGKTRAKAICAATGIAEDVK
                     IRELSEEQIEKLREEVGKFTVEGDLRREVTLSIKRLLDLGCYRGLRHRRSLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            complement(7680..7793)
                     /gene="rpmJ"
                     /locus_tag="PWX02_000632"
     CDS             complement(7680..7793)
                     /gene="rpmJ"
                     /locus_tag="PWX02_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDL1728670.1"
                     /translation="MKVRASVKRMCRNCKVIKREGVVRVICSDPKHKQRQG"
     gene            complement(7818..9137)
                     /gene="secY"
                     /locus_tag="PWX02_000633"
     CDS             complement(7818..9137)
                     /gene="secY"
                     /locus_tag="PWX02_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDL1728671.1"
                     /translation="MAKQPGYQGSTQKGTGELKSRLLFVLGALIVFRIGSFIPVPGID
                     ASVLSELIRQQQGTIIDMFNMFSGGALSRASIFALGIMPYISASIIIQLLTAIHPPLA
                     ELKKEGEAGRRKISKYTRYGTLLLAFIQSIGISLALPNMVHGLVPNPSILFYVTSVVS
                     LVTGTMFLMWLGEQITERGIGNGISLIIFAGIVADLPATIGQTIEQARQGELSFIVLL
                     LVAIIAFAVTYFVVFVERGQRRIVVNYASRQQGRMMAPARTSHLPLKVNMAGVIPAIF
                     ASSIILFPASITQWFGQSEGFEWLFNLSQLLQPGQPLYVVLFTMAVIFFAFFYTGMQY
                     NPRDTADNLKKSGAFVPGYRPGEQTSRYIDKVMTRLTLIGALYIAFVCLVPYIITSLW
                     KVPFQLGGTSLLIVVVVIMDFIAQVQSHLMSQQYDSVLKKANLKGLN"
     gene            complement(9140..9574)
                     /gene="rplO"
                     /locus_tag="PWX02_000634"
     CDS             complement(9140..9574)
                     /gene="rplO"
                     /locus_tag="PWX02_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDL1728672.1"
                     /translation="MRLNSLSPAEGAKHSAKRLGRGIGSGLGKTGGRGHKGQKSRTGG
                     GVRRGFEGGQMPLYRRLPKFGFTSLKSFHVAEIRLNDLAKVDGNEVTLETLKAANIIT
                     KDILSAKVILAGKIERAVVVKGLRVTKGAKAAIEAAGGSIEE"
     gene            complement(9579..9755)
                     /gene="rpmD"
                     /locus_tag="PWX02_000635"
     CDS             complement(9579..9755)
                     /gene="rpmD"
                     /locus_tag="PWX02_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDL1728673.1"
                     /translation="MAKIKVTQTRSSIARLPKHKATLKGLGLRHIRHTVELEDTPAVR
                     GMINQVSYMVKVEE"
     gene            complement(9762..10262)
                     /gene="rpsE"
                     /locus_tag="PWX02_000636"
     CDS             complement(9762..10262)
                     /gene="rpsE"
                     /locus_tag="PWX02_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDL1728674.1"
                     /translation="MSNIEKQAGELQEKLIAVNRVSKTVKGGRIMSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNEGTLQHPVKGSHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVRNVLSKAYGSTNPINVVRATIDALENMKSPEMVAAKRGKT
                     VEEILG"
     gene            complement(10277..10630)
                     /gene="rplR"
                     /locus_tag="PWX02_000637"
     CDS             complement(10277..10630)
                     /gene="rplR"
                     /locus_tag="PWX02_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDL1728675.1"
                     /translation="MDKKVARIRRASRARHLMREQGATRLVVHRTPRHIYAQVIAPNG
                     SEVLAAASTVEKVIKEQVKYTGNKEAAAVVGKIVAERAIAKGIKAVAFDRSGFKYHGR
                     VQSLADAAREAGLQF"
     gene            complement(10645..11178)
                     /gene="rplF"
                     /locus_tag="PWX02_000638"
     CDS             complement(10645..11178)
                     /gene="rplF"
                     /locus_tag="PWX02_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDL1728676.1"
                     /translation="MSRVAKAPVNIPAGVEVKLNGQLLTVKGKNGELSREIHNAVEVK
                     QEADALTFAPREGIANADAQAGTARALVNNMVIGVTEGFTKKLQLVGVGYRAQMKGNA
                     VALSLGFSHPVEHTLPAGVTGECPSQTEIILKSADKQLIGQVAADIRAYRKPEPYKGK
                     GVRYSDEVVRTKEAKKK"
     gene            complement(11195..11587)
                     /gene="rpsH"
                     /locus_tag="PWX02_000639"
     CDS             complement(11195..11587)
                     /gene="rpsH"
                     /locus_tag="PWX02_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDL1728677.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKVAISMPSSKLKVAIANVLAEEG
                     YIESVKVVEGVKPELEITLKYFQNKPVVESIQRVSRPGLRIYKRKDELPKVMGGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            complement(11624..11929)
                     /gene="rpsN"
                     /locus_tag="PWX02_000640"
     CDS             complement(11624..11929)
                     /gene="rpsN"
                     /locus_tag="PWX02_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL1728678.1"
                     /translation="MAKQSMIARDVKRAKLADKFYAKREELKKIISDVNASDEDRWAA
                     VLKLQTLPRDSSPVRQRNRCRQTGRPHGVLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            complement(11941..12480)
                     /gene="rplE"
                     /locus_tag="PWX02_000641"
     CDS             complement(11941..12480)
                     /gene="rplE"
                     /locus_tag="PWX02_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDL1728679.1"
                     /translation="MAKLHDYYRDTVVNELKAKFNYSSVMQVPRIEKITLNMGVGEAL
                     TDKKLLDNAVADLAAISGQKPLVTKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLNAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTSA
                     KTDEEGQALLAAFNFPFRK"
     gene            complement(12498..12809)
                     /gene="rplX"
                     /locus_tag="PWX02_000642"
     CDS             complement(12498..12809)
                     /gene="rplX"
                     /locus_tag="PWX02_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDL1728680.1"
                     /translation="MAAKIRQNDEVIVLTGKDKGKRGKVTKVLPNGKVFVEGINIITK
                     HEKPVPALGKAGGLVKKEAAIDVSNVAIFNLETNKADRVGFRFEDGKKVRFFKSSEKT
                     I"
     gene            complement(12820..13191)
                     /gene="rplN"
                     /locus_tag="PWX02_000643"
     CDS             complement(12820..13191)
                     /gene="rplN"
                     /locus_tag="PWX02_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDL1728681.1"
                     /translation="MIQEQTMLDVADNSGARSVMCIKVLGGSHRRYAAIGDIIKVTVK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGAVIRFDGNACVILNNNTEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            complement(13414..15249)
                     /gene="glmS"
                     /locus_tag="PWX02_000644"
     CDS             complement(13414..15249)
                     /gene="glmS"
                     /locus_tag="PWX02_000644"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDL1728682.1"
                     /translation="MCGIVGAVAQRDIAEILVDGLHRLEYRGYDSAGVAVLNADDKTM
                     QIVRRVGKVQALDEALDTKPLLGGTGIAHTRWATHGEPSETNAHPHRSGKIAVVHNGI
                     IENYEELKIVLQERGYVFQSQTDTEVIAHLVEWELRSASSLLEAVQKTVVQLRGAYGT
                     VVLNEEEPEHLIVARSGSPLVIGYGVGENFLASDPLALLSVTRRFAYLEEGDVAEITR
                     RTVDIYDREGNKVEREIHEGNFEADAADKGQYRHYMQKEIFEQPVAIMNTLDGRIKDG
                     KVDISAIAPNAAEILSKVEHVQIVACGTSYNAGMVSRYWFESIAGVSCDVEIASEFRY
                     RKFVTRPNSLLITLSQSGETADTLAALRLAKESGYMAAMTVCNVASSSLVRESDFAFL
                     TKAGVEIGVASTKAFTTQLTCLLLLNVAIGRLQGRMSEEQEHHIVQSLQRLPAQIESA
                     LVFDKQIEKLSEDFADKHHTLFLGRGEYYPIAMESALKLKEISYIHAEAYAAGELKHG
                     PLALIDSDMPVVVVAPENDLLEKVKSNIEEVRARGGQLYVFADHDAGFEEAEGFKTIV
                     MPKVDEVTAPIFYTVPLQLLSYHIALIKGTDVDQPRNLAKAVTVE"
     gene            complement(15297..16058)
                     /locus_tag="PWX02_000645"
     CDS             complement(15297..16058)
                     /locus_tag="PWX02_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDL1728683.1"
                     /translation="MSKRNTQQRRHLIVQMVQEKNEVSVDELAHFFETSEVTIRKDLT
                     ALEESGFLLRKYGGAVKIPSEMMEEAFIEQLSIQKKMIAQAARDCIRDHNRIIIDSGS
                     TTGALVKALNQTGLVVMTNSLNLAAELTSLECEPTVLMTGGTWDSRSESFQGKVAEQV
                     LRSYDFDQLFIGADGLDLARGTTTFNELVGLSQVMAEVSREVIVLIESQKIGRKMPNI
                     ELEWQNITKIITDSQLSFETKEQIEKMGVEVVIAK"
     gene            complement(16215..16415)
                     /locus_tag="PWX02_000646"
     CDS             complement(16215..16415)
                     /locus_tag="PWX02_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDL1728684.1"
                     /translation="MKSILKVVGAMVLMVAVSGCNNMTKTQRNTAIGAAIGGVAGHAI
                     GESTGATLGGAALGGVIGSQIK"
     gene            16640..17674
                     /locus_tag="PWX02_000647"
     CDS             16640..17674
                     /locus_tag="PWX02_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1728685.1"
                     /translation="MKLKKLSLALSTLLLGSAFSNSVLAEGRLTVYCSAANELCEQEV
                     QAFGKKYDVKVAFVRNGSGSTLAKIEAEKNNPQADVWYGGTLDPHSQAAEMGLLEPYK
                     SPNLEQIIEKFRDPAKLKGNYSSAVYMGILGYGVNLDRLKKLGIEKVPSTWEELLDPR
                     LAGEIQIADPQSSGTAYTAIATFVQLWGEEKAFDYFRKLHKNISQYTKSGITPARNTA
                     RGETTIGIGFLHDYAIEKKNGANIEMTAPTDGTGYELGGVSILKGARNLDNAKLFVDW
                     SLSKEAQELSWQKGNAFQVLTNTTAEASPYALDPKNLNLINYDFEKYGSSEERKRLID
                     KWVNEVKLAK"
     gene            17742..19778
                     /locus_tag="PWX02_000648"
     CDS             17742..19778
                     /locus_tag="PWX02_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016534092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL1728686.1"
                     /translation="MQNFSFSRPLVWVFIGLLGFLLLPSKALDYGLFDSTSDELLAAM
                     GWSSINITWAWFLPLLVFLLPNSTACKTRAKLELVGLGVLFAFIFLSAIVLKISLGYA
                     VLPLIIAIMGLATQNLATLKVMQGDRFIIGSLLSIILLIFFFIVYPTIAIFVSMFYNG
                     SEFVPSQVLTILQQPYVVRVIGNSLSVAGTVGVLSTLFGLAFALYTTRIAKRSAFIGR
                     IFSILPIVTPPFVVGLGVTLMLGRSGYVTEFLVDYFGFANNWLYGFTGIVIAHTLALT
                     PMSFMILEGALKSIHPSVEEAAYTLRSNRYQAFFNIIFPLLKPALANSFLVVAIQSLA
                     DFSTPLVLGGSFDVIATQIYFYIAGSQLDYASASTLGAILLVFSLAIFVIQYLWIGNR
                     SYVTVSGKSYRGDVQDLPSGMKWLIICTLLFWGTFNLVLYGSIFYGSFTVNWGVDYTL
                     TLDNYIKLFGQGFSDGGFPSLIQTVIFAAAAAPITALFGLLIAYVTVRRDFKGKKTLE
                     FLTLLCFAVPGTVAGVSYILAFNDAPIYLTGTGMIIILSMVMRNMPVGMRAAIAGLRQ
                     LDKSLDEASLSLKAGSFKTMLFVVLPLLKPALLSALVTSFVRAMTTVSAIVFLVTADT
                     RVATSYILNRVEDGEYGIAIAYGSILIVVMMAIILIFDWIVGDTRIARSKAKKM"
     gene            19876..20925
                     /gene="fbpC"
                     /locus_tag="PWX02_000649"
     CDS             19876..20925
                     /gene="fbpC"
                     /locus_tag="PWX02_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HDL1728687.1"
                     /translation="MNNDFLVLKNVTKSFGKVTVIDNLNLSIPQGKMVTLLGPSGCGK
                     TTVLRLVAGLENPTSGQIFIDGKDVTQSSIQHRDICIVFQSYALFPHMSIGDNVGYGL
                     KMQGVPKEKRLQRVKEALELVDLGGFEDRFVDQISGGQQQRVALARALILKPKVLLFD
                     EPLSNLDANLRRSMREKIRELQQRLNITSLYVTHDQSEAFAVSDEVIVMNKGKIMQKA
                     PAKELYLRPNSLFLANFMGESSIFEGTIAGNQININGYQFTLSNAEQFELPDGECLVG
                     IRPEAIHLAEQGNISQLCKIKNAVYMGNHWEIIAEWEGKDLLVNTKPENFYPHIKQAY
                     VHFIEQGVFLLKKEL"
BASE COUNT         5966 a         4730 c         4166 g         6206 t
ORIGIN      
        1 ttccaataaa aggttatatt tacagaatga atacgagact aaataatgca gcagaaaggt
       61 tttggaataa tttacaaaat attcagttta taaatgaaga tatattaaat aaaccctaac
      121 attctataat aagaaaaagc ggtcgattct ctgccaaatt ttgcaaattt tagaagaaaa
      181 cagaccgctt gtcagggttg gaattagagt aaccagctgc caatcaatgc acaggcaaaa
      241 ccggttaaac cgatggaggt ttcaagcatt gtccaggttt tgagggtggt tttttcgtcc
      301 atttcgagga aacggctcat cagccagaaa ccggagtcat tgacgtggga aagcacggta
      361 gcaccagagg caatggcaat cacgataaag cagaggtcga actggcttaa atcggtggct
      421 gctgcgacag ttggtgcaat taacgccgct gtggtggtta aggcaaccgt tgccgagcct
      481 tgtacaacac ggaaaacggt ggcaataata aaggcagcca ccacaatcgg cattccggtg
      541 tctttcatca tttccgataa cacatcgcca atcccgctgg cacgcaacac gccaccaaac
      601 attccgccag caccggtcac taagacaatc gagcagattg ggccaagggc attgttacag
      661 attttttcga tttggtcgaa actgcgatta tcccgcaaca gcataatcgc cacaattaag
      721 gtaatcagta aagcaacagg ggttttgccg attaagcgaa ggctctgcac ccacatttca
      781 ctgccatcaa tcactttggc aaccgagagg gtattcaagc cggtatcaaa caaaatcagg
      841 ataatcggca gcaccaacac cgtgagtacc ttgccgaaac ttggcggatt ttgcactgcc
      901 atttcgtttg ccgataagcc gtttaaaaag gctttcggta gatcaacgtg aatgcgtttg
      961 ctgataaatt ggctgaacag ataagtgccg atataccacg ttgggattgc acaaaccaag
     1021 cccaccaaga ctaacagccc catatttaca ccaagcagat cgccggaggc caccgggccg
     1081 gggtgtggcg gtagaaaagc gtgcattacc gcaaacgcac cggcagacgg aaacgcataa
     1141 cgcaataccg agccaccgaa ctgtttcgcc acactaaaaa taatcggcaa catcaccacc
     1201 aagccggcat caaagaaaat agggaagccg aataacagcg aggcaacacc caatgtaaag
     1261 ggtgctttct ctttaccgaa tttattgata agcgtatcgg caagcacttt tgcaccaccg
     1321 gtgatttcaa gcaagcgacc aatcatcgcc ccaagcccga ccagtaacgc cactgaagca
     1381 agcgtgctgc caaagccgtt taacaaagtt ggcaggattt tatccaccgg aataccggca
     1441 gctaatgcag tgagcaggct cacaataatc aatgccacaa aagcgtggac tttaaatttc
     1501 ataatcagca ccagcaataa aatgatggca gcaaacataa tgaacagtaa cataggatct
     1561 cctaaggttg aattgttatc ggtaacataa cccaaaagca gtttagagta aacggttgca
     1621 gggcttttat tttaaaaatg tgatctactt caagttttct ggaaatagca gatgattttt
     1681 cagcataacc tgttacatta acggctaagt taacgttacc agtaacacaa ggaaattcaa
     1741 tggcaaacgg caaagcattt attttgatgg gcgtgtccag caccggcaaa accagcgtgg
     1801 gaacggcagt cgcacagcgg ttgggaataa aattgattga tggggacgac ttacaccctc
     1861 gtgccaatat actgaaaatg gcaagtggca cgccactcaa tgatgcagat cgcacaccgt
     1921 ggttggagcg gattaacgat gcagtgttca gttttgaaca aaaaagcgaa aaagggatta
     1981 tcgtctgctc agcattgaag aaaaagtacc gagaccagat ccgacaaggc aatgccgatg
     2041 tgacatttct attccttcac ggctcatttg atctggtgct ggctcgaatg caacaacgta
     2101 aagggcattt tatgaaaccg gaaatgctcc aaagtcagtt caatacactg gaaattccac
     2161 aagcagacga gccagatgtg atttttattg atattgacgg cagttttgat gaggttgtag
     2221 agcgttgcat aaaggctgct gagccgttga tttaatcaca agctgttgat tttgcagatt
     2281 tttttgcaaa tttaaccgct tgcctttcct acacactttc ccccaaatga atgtgatagc
     2341 ccaagttaat tattgggctt tgttgcggta cgccgtgaat gcggtcgagg agttcttggg
     2401 cggcaactct gcccatttca aggcggggcg tcattacgct ggcaagctgt ggcgtgaggg
     2461 attgcccaac atcgtggccg tgaaagcctg cgattttaat ctgctgcggc acattaatgc
     2521 ctaaccgttg acactcaaat aatgcaccga ttgccaagtc gtcattagtg caaaaaatgc
     2581 cgtccagttg tggaatttgc tccaaagctt gttttagctg gcttgcgcca agcgtgaaag
     2641 aagaatgctc tgcagtagta atgctgtgtg ggcttaagcc gtgtttgagc attgcctgtt
     2701 cgtagccctg cattttcagc tgagagcgtt tatccattct cgccccgaaa tacaccacct
     2761 gtcggcagcc tcgatgaatc attgtttcta ccatcgcttg agccgcagaa atattgtcaa
     2821 atccaaccgc ttgctgaatc ccacattcac cactttccat tatctctatt accggaatat
     2881 tcgccacctc cagcattttc aaggtgcggg gcgagtggtg attttccgat aaaataatgc
     2941 cgtcaatatt gtacgaaagc agcgactcaa tccgctgttc ttccttctgc tcgctgtaac
     3001 catagtgggc aagcatagtt tggtagccgg cttggtcggt aatctgctca atgcctttaa
     3061 tcacatcagc aaacacttgg ttggtaagcg agggaaccag cacgccaatc gctcgacttt
     3121 tggcattaga gagaatatcc ggtgcacggt ttgggatata gccaaacagc tcaatcgctt
     3181 gtgcaatccg ttcgccggtt tctttggcaa ccgtgtttgg atctcgcaaa aaacggctaa
     3241 ccgtcatttt agtgatgccg agatgagagg cgatgtcttg caaagttggg cgtttcggtt
     3301 tactcattgg aatagataac gaaaaaattt aattttgctg attataactg aagataaggc
     3361 aataattgca aaatattcgg tgaaattaac cgcttgttac tactcttgcc tgaactcttt
     3421 cggcgttttg ccaaattctt ttttaaacac ggcatagaaa tattgtaaag agggataacc
     3481 acacacattg gcaatttctt gggtcgaaat ctcggtaaat ttgagcatat attttgctct
     3541 tgctaatttt tcttcgtgaa tcacttggtg aatcgtctta ttcatttcct ctttaaaacg
     3601 ttgttcgaga ttagagcggg aaactttgag atgatccagc acttgctcta ctttaatacc
     3661 ttgacatgct cgatgacgaa taaagtgcat agcttgaatt accattgggt cttgtaaaga
     3721 tcggtaatcg gtagaacggc gctcctcaac ttttaccggt ggaattaaaa tcggcttaag
     3781 agaaacgggt aaattattta atttacgata cagtaatttc gcggcgtgat aaccaatttg
     3841 cgatgttccc tgtgtaactg aagaaagcga aacccgagag aggtattgaa tcagctcttc
     3901 attatcaatg ccgatcacgc aaagttgttc aggtacagca atttttaaat gctcgcaggt
     3961 ttgaagtaga tgtcttgccc ttgcatcagt taccgcaata ataccagtat gaggtggtaa
     4021 agtttgcaac cactcgccta atttagcttg taattccacc cagttgtctg aatggaattg
     4081 atcgccaata tagcaattcg gctgatgttc gtatttttcc atcaactgaa taaaggcatt
     4141 ttggcgttct atcgcccagt gtttttccgt ttctgccgga tagccgtaaa atgcaaattg
     4201 atctatacct ttctgtttta gatgcaaaaa tgcagcctca attaaggcgt aattatcggt
     4261 ggcaacataa ggaagattag ggtaatagtc ggagttttgg taagaaccac ctaccgccac
     4321 aatcgggaca tcaatatctt taagcaactc gaccgtttct aaatcgtcaa aatcggcaat
     4381 aataccgtca atcaacaggc tttcgatact gtttttatga tagacaaaat cgtcttccac
     4441 aaaaatatcc caagtacatt gtgaggcttg aataaactgc ccgatgcctt gcattactcc
     4501 cctgtcataa attttgttag cattaaaaag taaggcaatt ttataagatt gttctatcat
     4561 tacttcctcc agtacccact ttcaaaagta actataaaat aaaattattt tttgatcaaa
     4621 gcgaataaaa gaataaaacc ccagtacttc tactggggtt ttaagctcaa ttttgattaa
     4681 gattattccg cagctgcttc agttgcaaca tctgggcgat ccactaactc aatgtaagcc
     4741 attggagcat tatcacctgc acggaaacca cattttaaga tgcgagtgta accacccgca
     4801 cgttgtgcaa aacgtggacc taattcattg aataatttag caactgtatc aacgttgcgt
     4861 gtacgggcaa aagctaaacg gcgatttgca acgctgtctg ctttagctaa agtaattaac
     4921 ggctcaacta cacggcgtaa ctctttcgct tttggtaaag ttgtcttgat gatttcgtgt
     4981 tcaacaagcg cacttgccat attacggaac atcgcttggc gatggctgct gttacggttt
     5041 aattgacgtc cactcttacg atggcgcata acctattcct tcttagaaaa tctataccga
     5101 aaaactagtc ttctgcaata cttgcaggtg gccagttttc aaggcgcata ccgagtgata
     5161 aaccacgaga agcaagaaca tccttaatct ctgtaagaga ttttttaccc aagttcgggg
     5221 ttttaagaag ttcaacttct gtacgttgta ctaaatcacc gatatagtga attgtctctg
     5281 ctttcaaaca gttagcagaa cgaactgtca gctctaggtc atctactgga cgaagaagaa
     5341 taggatcaaa ttccggtttt tcttctttca cttcaggttg acgaacatca cgcaaatcaa
     5401 cgaatgcagc aagttgttct gctaaaatcg ttgcagcacg acggatagct tcttcaggat
     5461 ctaacgttcc gttagtttcc atctcaataa cgagtttatc taagtctgta cgttgttcaa
     5521 cacgagcagc ttcgacattg taagcaatgc gatctacagg tgaaaaacgt gcatctacta
     5581 ataaacgacc gataggacgg tcatcgtttt gagtatgaag acgtgctgat gcaggaacat
     5641 aacctcgacc acgttgaaca cgaatgcgca tattaattga cgcattctta tctgttaaat
     5701 gacagattac gtgatcagga ttcacaattt ctacatcgcc atcgtgtgtg atgtcggctg
     5761 cagttacagg gccaattcca gatttattta gtgtcaaaat aatatcatct ttattttgca
     5821 cttttaccgc tagaccttta aggtttaata atacctcaag aatatcttcc tgaacgcctt
     5881 ctttgctgct gtactcatgt agtacaccat caatttcaac ttcagtaact gcacaacccg
     5941 gcatagatga aagtaaaatg cggcgaagtg cgttacctaa tgtatggcca aaaccacgtt
     6001 ctaacggctc taatgtcact ttagcatgag ttgaactaac ttgttcaata tttactaagt
     6061 gcggctttaa aaattctgtc acagaaccct gcattttttc ctctctttgc ggttaagcta
     6121 actattattt agagtaaagc tcaacgatca gatgttcatt aatatctgct gatagatcag
     6181 aacgctctgg acgacgttta aatacgcctt ccattttagt tgcatcaact tctaaccaag
     6241 ttggtttttc acgttgagtt gctaattcta atgatgcttt aatacgtgct tgttttttcg
     6301 atttttcacg aacagcaaca acatcatcaa cagaaacttg gaatgaagga atgtttacta
     6361 cacgaccgtt tactacgatt gatttatggc taacaagctg acgtgcttct gcacgtgttg
     6421 ctgcaaaacc catacgataa actacgttat ctaaacgacc ttccaataat actagtaagt
     6481 tttcaccggt attacctttt aagcggttcg cttctgtata ataattacgg aattgacgct
     6541 ctaagatacc ataaatacgg cgaacttttt gtttttcacg taactgacta ccatagtcag
     6601 ataaacgtgg cttgcgagca ccatgttgac caggcgctac atcaagtcta tttctacatt
     6661 ttgattcaat cgcacgaacg cctgatttta agaataaatc agtaccttca cgacggctta
     6721 gcttgagctt aggacccaaa tatcttgcca ttttctttct ccaattcttt taacgtcaat
     6781 taaacacgac gttttttcgg tggacgacaa ccgttatgtg gaatcggagt cacatcagta
     6841 atattcgtga tacggaaacc tgctgcattt aatgcacgga ttgttgactc acgacctgga
     6901 cccggtcctt taaccataac ttccaaattc tttaaaccaa actctttaac catttctgca
     6961 caacgctctg cagcaacttg agcagcgaat ggagtcgatt tacgagaacc acggaaacct
     7021 gaaccacctg cagttgccca agctagagca ttaccttggc ggtcagtaat agtaacgatt
     7081 gtgttattga aagatgcgtg gatatgagct acgccatctg caatctgttt ttttacgcgc
     7141 ttacgtgcgc gaactggtgt tttagccatt atttatctac tccgactatt ttttgatcgg
     7201 tttgcgtgga cctttacggg tacgcgcatt agtcttagta cgttgaccac gtaccggtaa
     7261 actacgacga tgacgtaaac cacggtagca acctaagtct aaaagacgtt tgatgcttaa
     7321 agttacttca cgacgtaaat ccccttcgac agtaaattta ccaacttctt cacgcagttt
     7381 ttcaatctgc tcttcagaca attctctgat ctttacatct tcggcaatac ccgttgcagc
     7441 acagatggct tttgcacgag ttttaccgat accgtaaatt gcagttaatg cgattacagt
     7501 gtgtttttga tcaggaatgt taatgcctgc aatacgggcc actatgcact cctattattt
     7561 aagtttattg atatactgat cttgaaaagc ccgtttcagg atactcaaac agtatatcag
     7621 gacgaatgag ctgagcagta taactgctca gcgggttctt tgcaagaaat atgcgtaaat
     7681 taaccttgac gttgtttgtg tttaggatcg ctacaaatta cacgaaccac accttcacgt
     7741 ttgattactt tacaattacg gcacattctt tttactgaag cacgaacttt cattgcttat
     7801 cctttattaa cggcctatta atttagacct tttaagttag ctttcttcaa cacagaatca
     7861 tattgttgag acattaaatg actctgaacc tgtgcgatga aatccataat aacaaccact
     7921 acaatcaata atgatgtacc acctaattgg aatggcacct tccataacga cgtaatgatg
     7981 taaggaacca aacaaacaaa agcaatatat aacgcaccaa ttaaggttaa acgtgtcatt
     8041 actttatcga tataacgaga tgtttgttcg cctggtcggt aacctggtac aaacgcacct
     8101 gatttcttaa gattatccgc tgtatcacga ggattatact gcattcctgt ataaaagaaa
     8161 gcaaagaaaa tcactgccat tgtgaataat acaacataca gtggttgacc tggctgtaat
     8221 agttgtgaaa ggttaaataa ccactcaaat ccttcgcttt gaccaaacca ttgagtaata
     8281 cttgccggga ataaaataat acttgaagca aaaattgccg gtattacccc tgccatattc
     8341 acttttaatg gtaagtgaga agttcttgca ggcgccatca ttcgaccttg ttggcgactt
     8401 gcatagttta caactattct tctttgtcca cgttcaacaa aaacaacaaa ataagttact
     8461 gcaaatgcaa ttattgcaac tagtagtaag acgataaatg ataactcacc ttgacgggct
     8521 tgttcaattg tctgccctat cgttgctgga agatctgcaa caatacctgc aaaaataatc
     8581 aatgagatac cgtttccgat accacgctca gtgatttgtt cgcctagcca cattaggaac
     8641 attgttcccg tcactaagct tactacagaa gttacataga ataatatact tggattagga
     8701 actaatccat gtaccatatt cggtagggct agtgaaatac caatagattg aataaatgct
     8761 aataacagtg tgccatagcg tgtatattta ctgattttcc gacgacctgc ttcaccttct
     8821 ttctttaatt cagctagagg tgggtgaatc gctgtcaata attggataat aattgacgct
     8881 gaaatataag gcataatacc tagtgcaaat atagatgcac ggcttaaagc accaccagag
     8941 aacatattaa acatatcaat gatggtgcct tgctgttgtc gaattaattc ggataataca
     9001 gaagcatcaa tgccaggaac aggtataaaa gaaccgatac ggaaaacgat taacgcacct
     9061 aaaacaaaca atagtcttga tttaagctcg ccagtccctt tttgtgtact accttggtac
     9121 cctggttgct ttgccatctt tattcctcga tagaaccgcc agcagcttca atagcagctt
     9181 tagcgccttt agttacacgt aagcctttaa caactactgc tctttcaatt ttgcctgcta
     9241 aaattacttt agcagataaa atatctttag taatgatgtt tgctgctttt aatgtctcta
     9301 aagttacttc gttgccatct actttagcta agtcgtttaa acgaatttca gccacatgga
     9361 atgattttaa tgaagtaaaa ccaaattttg gcaaacgacg atataaaggc atttgaccac
     9421 cctcgaaacc acgacgaaca ccgccgcctg tacgagattt ttgaccttta tgaccgcgcc
     9481 caccagtttt acctaaacca gaaccaatac cacgacctag acgttttgca ctgtgcttag
     9541 caccttcagc tggagaaaga gaatttaaac gcattctatt actcctctac tttaaccata
     9601 taagacactt gattgatcat accacgcact gctggtgtat cttcaagttc tacagtatga
     9661 cgaatatgac gaagacctaa gccttttaac gttgctttat gtttcggtaa acgagcgata
     9721 gagctacgag tttgagttac tttaattttt gccatatcca attaccccaa aatttcttca
     9781 actgttttgc cacgttttgc agcaaccatt tcaggtgatt tcatgttctc aagtgcatcg
     9841 attgttgcac gaacaacgtt aattgggtta gttgaaccat acgctttaga aagaacgtta
     9901 cgaacacccg caacttctaa tactgcacgc attgcaccac cagcgatgat acctgtacct
     9961 tcacttgctg gttgcataaa tacgcgtgaa cctgtatgtg aacctttaac tggatgttgt
    10021 aatgtacctt catttaaagc tacattaatc atattgcgac gagctttttc catcgctttt
    10081 tggattgctg ctggaacttc acgtgcttta ccgtaaccaa aacctacacg accgttacca
    10141 tcgcccacta ctgttaaagc agtgaaactc ataatacgac caccttttac ggtttttgaa
    10201 acacggttaa ccgcgattag cttttcctgc agttcaccag cttgtttttc gatgtttgac
    10261 atctcaaatt cctctattag aactgtagac cagcttcacg agcagcatct gctaatgatt
    10321 gcacacgacc gtggtattta aaaccagaac ggtcaaaagc aaccgcttta atacctttgg
    10381 caattgcacg ctcagcaacg attttaccca ctactgctgc ggcttcttta ttgccggtat
    10441 atttaacttg ctctttaatt actttttcaa cagttgaagc tgctgctagt acttctgagc
    10501 cattaggtgc aatcacctgc gcatagatat ggcgaggtgt gcgatgcaca actaaacgtg
    10561 ttgcaccttg ctctctcatt aaatgacgtg cacggcttgc acgacggata cgagctactt
    10621 tcttatccat agtgttacct tactttactt tttcttcgct tcttttgtac gcactacttc
    10681 atcagagtaa cgtacacctt tacctttata aggttcaggt ttgcgatatg cacgaatatc
    10741 tgctgccact tgaccaatta actgtttgtc agcacttttc agaataattt ctgtttgtga
    10801 tggacattca ccagttacgc ctgctggcaa cgtgtgttct actgggtgag agaaacctaa
    10861 acttaaagca acagcattgc ctttcatttg tgctctataa ccaacaccca ctaattgcaa
    10921 tttcttagta aagccttcag taaccccgat taccatatta tttactagtg cacgagcagt
    10981 acctgcttga gcatctgcat tagcgatacc ttcacgtgga gcgaaagtta atgcatctgc
    11041 ttcttgtttt acttcaactg cattgtgaat ttcacgagat aactcgccgt ttttaccttt
    11101 aactgttaat agctgaccgt taagttttac ctcaacaccg gcaggaatat taacaggtgc
    11161 ttttgcaaca cgagacattt ttcctacctc tctattatgc tacgtaacag ataatttcac
    11221 cgccgaggcc cgcttgacgc gcagcacggt cggtcattac acctttagat gtagaaacaa
    11281 ctgcgatacc taaaccaccc attacttttg gtaattcgtc tttacgttta taaatacgca
    11341 gacccggacg gctcacacgt tggatacttt ctacaaccgg tttgttttgg aaatatttta
    11401 aagtaatttc cagttcaggt ttaacacctt caacaacttt aacgctttcg atataacctt
    11461 cttcagctaa aacatttgca atagcaactt ttaacttaga tgaaggcata ctgattgcaa
    11521 ctttgttcgc agcttgaccg ttacgaatac gggtcagcat atctgcgatt ggatcttgca
    11581 tgctcatgta tttactccca tgattccaaa ttaaaagagg ttattaccag cttgccttct
    11641 taagacccgg aatttcaccg cgcatagcag cttcacgaac cttaatacgg cttaaaccaa
    11701 acttacgaag tacgccatgt ggacgaccag tttggcggca acgattacgc tgacggactg
    11761 ggctagaatc acgaggaagt gtttgtaatt ttaacactgc tgcccaacgg tcttcatcag
    11821 atgcattcac atcagagatg attttcttta attcttcacg ttttgcgtag aatttatcag
    11881 ctaatttagc acgttttaca tcgcgtgcga tcattgattg ttttgccacg ataacctacc
    11941 ttatttacgg aatgggaaat tgaaagcagc taataaagct tgaccttctt catcagtttt
    12001 cgctgaggtg gtgatagtaa tatctaaacc acgaacacga tctactttat cgtagtcgat
    12061 ttcagggaaa atgatttgtt cacgaacacc catactgtaa ttaccacgac catcgaatga
    12121 cttagcgttt aaaccgcgga agtcacgaat acgtggaaca gcgatagtaa tcaatctttc
    12181 aaagaattcc cacatacgtt caccacgtag ggttactttg cagccgattg gatacccttg
    12241 acggatttta aagcctgcaa cagatttgcg agctttagtt actaaaggtt tttgaccgct
    12301 gattgctgct aaatccgcta ctgcgttatc taacagtttt ttgtcggtca atgcttcacc
    12361 cacacccatg ttaagggtaa tcttttcgat tcgtgggact tgcatgacag atgagtagtt
    12421 gaattttgct tttaattcat taactactgt atctctgtag taatcatgca gtttcgccat
    12481 cacattactc cagttaatta aattgttttc tcgctagatt tgaagaaacg cacttttttg
    12541 ccatcttcga atctaaatcc tacacggtca gctttgttgg tttcaaggtt gaagatcgca
    12601 acgtttgaaa catcgattgc agcttctttc ttcactaaac caccagcttt gcctaatgca
    12661 ggaactggtt tttcgtgttt agtgatgatg ttgataccct caacaaaaac tttaccattt
    12721 ggtaacactt tagttacctt accacgtttg cccttatctt taccggtaag aacaattact
    12781 tcatcgtttt gacggatttt tgcagccatt acattctcct tatagtactt ctggagctaa
    12841 agagatgatt ttcataaact tctcagaacg aagttcacga gtcaccggtc caaaaatacg
    12901 agtaccgatt ggttgctcag tgttattgtt taaaattaca caagcattgc catcgaaacg
    12961 aataactgcg ccatctgggc gacgaacacc cttcttggtg cgcacaacaa ctgcttttaa
    13021 cacatcacct tttttcactt taccgcgtgg aattgcttct tttacagtaa ctttaatgat
    13081 atcgccaata gcagcgtaac gacggtgcga tccacctaga accttgatac acattacgct
    13141 acgagcccct gagttatcag caacatccag catagtctgt tcttggatca tattagtgct
    13201 ccgttaaaat aaaaaaacac cctttcggga cgaacctatt tatacaaata taaatagggt
    13261 gcaaaatata ctacaaaaac cactagttaa tcaagtgaat ttttaagcta atttaatagt
    13321 ttaagtaagc ttatttataa attgtttcaa ctatcaataa actgtcctat aactcatata
    13381 aaaaccccaa gatatcttgg ggtttaacta acattactct acagtcaccg cttttgcaag
    13441 gttacgaggt tggtcaacgt ccgttccttt gattaaggca atgtgatatg aaagtagttg
    13501 taacggcacg gtgtagaaaa tcggtgcggt tacttcatca actttcggca ttacaatcgt
    13561 tttgaagcct tctgcttctt caaaacctgc atcgtgatcg gcaaacacat ataactgacc
    13621 gcctcttgca cgcacttctt caatattgga tttcactttc tcaagcaagt cattttccgg
    13681 tgctaccacc actaccggca tatcgctatc aattaatgct aacgggccgt gttttaactc
    13741 gcctgcggca taagcttctg cgtgaatata tgaaatctct tttaatttca atgcagattc
    13801 catcgcaatc gggtaatatt ctccacgacc taagaacaat gtgtggtgtt tatccgcaaa
    13861 atcttccgat aatttttcga tttgtttatc gaacactaac gcactttcaa tttgtgctgg
    13921 taagcgttgt aaagattgca cgatatggtg ttcttgctct tcactcattc tgccttgtaa
    13981 gcgtccaatc gccacattta ataacaataa gcaagttaat tgagtagtga atgctttggt
    14041 tgatgctaca ccaatttcaa caccagcttt ggttaagaat gcaaaatcgg attcacgcac
    14101 taatgaagaa cttgccacat tacacaccgt cattgccgcc atatagccag attctttcgc
    14161 taaacgcagt gcagctaacg tatctgcggt ttcacctgat tgtgaaagcg tgattaataa
    14221 gctgttcggg cgagtaacga atttacggta acggaattcg gaggcaattt ccacatcaca
    14281 gctcacgcct gcaatggatt caaaccaata acgtgaaacc ataccagcat tgtaagaggt
    14341 tccacaagcc acaatttgca cgtgttctac tttcgataaa atctctgccg cattcggtgc
    14401 aatggctgaa atatctactt taccgtcttt gatacgacca tctaaggtat tcataatcgc
    14461 aaccggctgc tcaaaaatct ctttttgcat atagtgacga tattgtcctt tatctgccgc
    14521 gtctgcctca aaattgcctt cgtgaatttc acgctctacc ttgttgcctt cacggtcgta
    14581 aatatccact gtgcgacggg taatttcggc tacatcacct tcttctagat aagcaaaacg
    14641 gcgagttacg cttaataatg caagtgggtc tgaagcaagg aagttctcgc ctacaccgta
    14701 accaatcact aacgggctgc ctgagcgagc cacaattaaa tgctccggct cttcttcatt
    14761 taataccact gtgccgtaag caccacgcag ttgcaccacg gttttttgta ccgcttctaa
    14821 taatgaagaa gcagaacgca attcccattc aactaaatga gcaatgactt cggtatcggt
    14881 ttgagattgg aacacataac cacgctcttg taacactatt tttaactctt cgtagttttc
    14941 gataatcccg ttatgaacta ctgcaatttt gccgctgcgg tgtgggtgag cattagtttc
    15001 tgacggctcg ccgtgcgttg cccagcgagt gtgtgcgatc ccagtgccgc ctaatagtgg
    15061 cttagtgtct aacgcctcat ctaaggcttg gactttaccg acacggcgta caatttgcat
    15121 tgttttatca tctgcattta atactgctac accagcagag tcatatccac ggtattctaa
    15181 gcgatgtaaa ccatcaacca agatttctgc gatatcacgt tgtgctactg cacctacaat
    15241 tccacacata aatttgcttt cctatgttta agtttaaaga ttcatttgag ctactgctat
    15301 tttgcaataa ccacttcgac acccattttt tcgatttgtt ctttcgtttc aaaacttaat
    15361 tgactgtcgg taataatttt ggtaatattt tgccattcca attcaatatt cggcattttc
    15421 cgaccgattt tttgcgattc aattaacaca atcacttcac ggctcacttc tgccatcacc
    15481 tgacttaagc caactaattc attaaaggta gtcgtgcctc gagccaaatc taaaccgtcc
    15541 gcaccaataa aaagctgatc gaaatcatat gaacgcaata cctgttctgc tactttgcct
    15601 tgaaaggact cagaacggct atcccacgta ccaccggtca tcaacactgt tggttcgcat
    15661 tcaagggaag tcaattccgc cgctaaattc agcgaatttg tcatcaccac caagccggtt
    15721 tggttcaatg cttttaccaa agctccggtc gtgctgccgc tgtcaataat gatgcgatta
    15781 tgatcacgaa tacaatcacg tgcggcttga gcaatcattt tcttttgaat cgaaagttgc
    15841 tcaataaacg cttcttccat catttccgat ggaattttta ccgccccacc atacttacgc
    15901 aataaaaaac cactttcttc caaagcggtt aaatcttttc taatcgtaac ttcagacgtc
    15961 tcaaagaaat gagcgagttc atctacactc acttcatttt tctcttgtac catttgaaca
    16021 attaaatggc ggcgttgttg cgtgttacgt tttgacattg taatcttcac ttaaatttcg
    16081 tttcaaaact tttgcattat aaagaaagct cgcgaaaaga aaagcagaaa atgcaaaatt
    16141 tacaaaatct atacaaaata aaaccgcttg taatattgat ttacaagcgg ttgatttctt
    16201 gaaatttttt gcaattattt gatttggcta ccaatcacgc caccaagtgc ggcaccacct
    16261 aaagttgcac cggtagattc accaatcgcg tgaccagcca caccgccaat tgctgcaccg
    16321 attgcagtat tacgttgcgt ttttgtcata ttgttacagc cagacacagc aaccattaat
    16381 accattgcac caacaacttt caaaatagat ttcataataa tccctcataa ttaatcaatt
    16441 tcaaataagc caatattctt ggctatgcta taagatttaa tttatttaga aaggttcaac
    16501 tactaccacc tatgaattgc aaattttttc acaatttagg ccgcttgttg attgaactgt
    16561 gagctcgctc acagaatttc atgacatttc ttgctatatt gtgtagcaat ctatgcgata
    16621 actcatcaag gaaataaaaa tgaaactgaa aaaattatct ttagcactct cgactttatt
    16681 actcggctca gccttttcca actctgtatt agcagaaggt cgcttaaccg tttattgtag
    16741 tgcggccaac gaactttgtg agcaagaagt gcaagcattc ggcaagaaat acgatgttaa
    16801 agtggctttt gtgcgtaacg gttcaggcag cacactggcg aaaattgagg ctgagaaaaa
    16861 caatccccaa gccgatgtat ggtatggtgg cacgctagac ccacattcac aagcggcaga
    16921 aatgggcttg ttagagccgt ataaatcgcc aaacctcgaa caaattatcg aaaaattccg
    16981 tgatccggca aaactaaaag gcaactactc ctccgctgtg tatatgggga ttttaggtta
    17041 cggcgtgaat ttagaccgtt tgaaaaaact cggcattgaa aaagtaccta gcacttggga
    17101 agaattgtta gaccctcgcc tagccggtga aatccaaatt gccgatccgc aaagctccgg
    17161 cacagcttat acagcaattg cgactttcgt gcaactttgg ggcgaagaga aagccttcga
    17221 ctatttccgc aaattgcata aaaacatttc gcaatatacc aaatccggca tcacaccggc
    17281 tcgcaacact gcccgtggtg aaaccaccat cggtatcggt ttcttacacg actatgctat
    17341 cgagaagaaa aacggtgcta atattgaaat gaccgcccca acagacggta caggctacga
    17401 attaggtggc gtgagtatcc tcaaaggagc aagaaacctt gataatgcca aactgttcgt
    17461 ggattggtcg ctttctaaag aggctcaaga actctcttgg caaaaaggca atgcgttcca
    17521 agtattaacc aacaccaccg ccgaagcctc gccttacgcc ctcgatccga aaaacttaaa
    17581 cttaatcaat tatgattttg agaaatatgg ctcaagcgaa gagcgtaaac gcttgatcga
    17641 taaatgggta aatgaagtaa aattagcgaa atagggttga tctttccact gaggggataa
    17701 gtgattatcc cctctctcat tataaaaatc tgtgaggtgt tatgcagaat ttttcctttt
    17761 cccgaccgct tgtttgggtg tttatcgggt tactcggctt tctcttatta ccgtccaaag
    17821 ccttagatta cgggctgttt gactcaacct ctgacgaact gctcgccgca atgggttggt
    17881 cgagtatcaa cattacttgg gcgtggtttt taccgctttt ggtattttta ttgccaaatt
    17941 cgaccgcttg caaaaccaga gccaagctgg aattagtcgg gttgggcgta ctattcgcct
    18001 ttattttcct gtcggcgatt gtgctgaaaa tcagtcttgg ttatgcggtg ttgccgttga
    18061 ttattgccat tatggggctt gcaacccaga atttagccac gcttaaagta atgcagggtg
    18121 accgttttat tatcggctcg ttactgagca ttattctgct gattttcttt tttattgtat
    18181 atccgaccat cgccattttt gtgtcgatgt tctacaacgg ctcggaattt gtgccaagcc
    18241 aagtgctaac gattttgcaa caaccttatg tcgttcgggt cattggcaac tcgctctcgg
    18301 ttgccggcac agtcggcgtt ctctccaccc tattcggctt ggcgtttgcc ctctacacca
    18361 cccgaattgc caaacgctca gcgtttatcg ggcggatttt ctcgattttg cccatcgtca
    18421 caccaccgtt tgtagtcggt ttaggcgtaa ccctaatgct tgggcgttcc ggttatgtga
    18481 ctgaatttct agtggactat ttcggctttg ccaacaactg gctgtacggt ttcaccggta
    18541 ttgtgatcgc ccataccctc gctctcacgc caatgtcgtt tatgattttg gaaggggcat
    18601 tgaaatccat tcacccatcg gtggaagagg cggcttacac cctgcgctcc aatcgctatc
    18661 aagccttttt taacatcatt ttcccattgt taaaaccggc gttggcaaac tcctttttgg
    18721 tggtggcgat ccaatccctt gccgatttca gtacgccgtt ggtgttgggc ggcagttttg
    18781 atgtgattgc aacccaaatc tacttctaca tcgccggctc ccaactggat tacgcctccg
    18841 ccagcacact cggggcaatt ttgctggtat tctccctagc cattttcgtg atccaatacc
    18901 tctggatcgg caaccgctct tatgtgacgg tgtcgggcaa atcctatcgg ggcgatgtgc
    18961 aagacttacc aagcggtatg aaatggctga ttatttgcac tttattgttc tggggaacgt
    19021 tcaatttagt gctatatggc agtattttct acggtagttt caccgtgaac tggggcgtgg
    19081 attatacctt aacccttgat aactatatca agctgttcgg gcaagggttt agcgatggtg
    19141 gtttcccttc gttaattcaa accgtgattt tcgctgctgc cgccgctcca attaccgccc
    19201 tatttggctt gctgatcgcc tatgtgacgg ttcggcgtga tttcaaaggc aagaaaaccc
    19261 tagagttttt aaccctgctc tgctttgccg tgccgggaac ggtggcaggc gtctcttaca
    19321 ttcttgcctt taacgatgcc ccgatttact taaccggcac cggtatgatc attattctct
    19381 caatggtaat gcggaatatg ccggtgggta tgcgggcggc gattgccggc ttgaggcagt
    19441 tagataaatc actggacgaa gcctcgctct cgctgaaagc cggttcgttc aaaacaatgc
    19501 tgtttgtggt gttaccgctg ctcaaaccgg ctctgctctc cgccctcgtc accagctttg
    19561 tgcgagcaat gaccaccgtg agtgcgattg tgttcttagt cactgccgac acccgtgtgg
    19621 caacctccta cattcttaac cgtgtggaag acggcgaata tggcattgcg attgcctacg
    19681 gctctatttt aattgtggtg atgatggcga ttatcctgat ttttgactgg attgtcggcg
    19741 acacccgtat cgcccgttct aaggcgaaaa aaatgtaaaa agacatctac tctctccctt
    19801 tatgggagag agacagcaaa atacttcgtt cagaagtatt tttcagagag agggatataa
    19861 ttaaaaggac aatatatgaa caacgatttc ttagttttaa aaaacgtcac caaatctttt
    19921 ggcaaggtaa cggtcattga caacctcaac ctcagcattc cgcaaggcaa aatggtgacc
    19981 ttacttgggc cgtctggttg cggaaaaacc acggtgctac gtttggtggc tgggctggaa
    20041 aacccaactt cggggcagat ttttattgat ggcaaagatg tgacccaaag ttcgatccag
    20101 caccgtgata tttgcattgt gttccagtct tacgctctgt ttccgcatat gtcgattggc
    20161 gataatgtcg gctacggctt gaaaatgcag ggcgtaccga aagaaaaacg gctgcaacga
    20221 gtgaaagagg cgttggaatt ggtggattta ggcggttttg aagatcgctt tgtcgatcaa
    20281 atctccggcg gacagcaaca acgtgtcgcc ctcgcccgtg ccttaattct gaaaccgaaa
    20341 gtgctactgt tcgatgaacc gctgagtaac cttgacgcta acttacgccg ttcaatgcgg
    20401 gagaaaatcc gtgaattaca gcaacgttta aacattactt ccctttatgt gactcacgat
    20461 cagtccgaag cctttgcggt ttcagatgaa gtaattgtga tgaacaaggg aaaaattatg
    20521 caaaaagctc cagccaaaga gttatacctt cgccctaatt ctttattctt agcaaatttt
    20581 atgggtgaaa gcagcatttt tgaaggcacg atagctggca accaaattaa tataaatgga
    20641 tatcaattta cgttatcaaa tgctgaacaa tttgagttac ccgatgggga atgcttagtt
    20701 ggcattcgtc cagaagctat tcatttagct gaacaaggaa atataagtca actctgtaaa
    20761 attaaaaatg cagtttatat gggaaatcat tgggaaatta tagctgaatg ggaagggaaa
    20821 gatttattag tcaacactaa acctgaaaat ttttaccctc atatcaaaca ggcttatgta
    20881 cattttatcg aacaaggggt ttttctttta aagaaagagc tataacgaat ttatactaaa
    20941 ttaagaggaa ggtagttata actatttagc aattttgaga aaaataggcc gcatgcaaaa
    21001 tctcttgatg acaagcggtc ttttcttgcc gattttttgc aaatgcaatg atatggctgg
    21061 cttaatcg
//
LOCUS       DANJWC010000023        20794 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.023, whole genome shotgun sequence.
ACCESSION   DANJWC010000023 DANJWC010000000
VERSION     DANJWC010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20794
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.023"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..911
                     /gene="terL"
                     /locus_tag="PWX02_000650"
     CDS             <1..911
                     /gene="terL"
                     /locus_tag="PWX02_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HDL1728688.1"
                     /translation="GTILHYDSVLNRVLSTKGWKKAHFKAILRFPDNMALWDEWENIY
                     LSEEGDDDTLSDLFYQQHKAEMDKGAVVSWLARPILYLMKIRASDGHSSFDSEYQNDP
                     VSGDDAIFANSIQYWTELPENLIYFGALDPSMGKAGASRDPSAILVGGYHRESGKLYV
                     VEAQIKKRLPDLIIEDVIRLHSQYNCHRWFVETVQFQEFLQTELVKRSAQRGKPVPAT
                     ATKPNSDKMLRIESLQPHIANGLILLHRSQSTLESQLRHFPKADHDDGPDALEMLWRN
                     ALSSSAPIEWESVDDFDGEYRSKWRH"
     gene            980..2653
                     /locus_tag="PWX02_000651"
     CDS             980..2653
                     /locus_tag="PWX02_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HDL1728689.1"
                     /translation="MSKKKHNKQIKPNLNPIKNQELQTDLAEITATGRVLSDHPSNFI
                     TPAKMKAIFEDAENGDITAQHELFMDIEERDSTIFANIQTRKRAALGVDWAIVAPRNA
                     TPQEEKLRDEVDELFYQLGNLEDFVMDCMDAVGHGFAALEIEWAFNGKLWYPNAFIHR
                     PQSWFKWDKVDNLLLKTRQNTEGEPLREFGWVVHTHKSRSTQAARNNLFRSLAWLYMF
                     KHYSIHDFAEFLELYGMPIRIGKYGAGATPNEKQTLKRALAEIGHNAAGIMPESMSIE
                     LHNAANAGGAAGNNPFLQMVDWCEKSIARLILGQTLTSGADGKSSTNALGNVHNEVRR
                     DLLVSDVKQLGQTFTQQIILPFLQINFGNIDPTRIPSFEFDTKEPADLALFAESLPKL
                     VDIGLPIPTQWAMDKLGIPEAQEGEAVLGRVAPLPTSGQAVGLSAHFVKSPPNPLFVK
                     EGEQITGCSCGCGGKVQALSAQPQTGIKEQDALDSLANEAFAVPDFNKQFDPITKKAV
                     AVVMACSSYDEAAEKLAAAYPDLVSEEHERYLANALFLADLLGAANAES"
     gene            2640..3929
                     /locus_tag="PWX02_000652"
     CDS             2640..3929
                     /locus_tag="PWX02_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDL1728690.1"
                     /translation="MQKVNFAIGLEPKQAIEFLRQKKLLANKVFKQELFDSALGRATT
                     ISKLTSLEMTRDIYESMEKARREGKSFNEWKKTLTAEFERKGWVYGHDKAISRGIDGK
                     LLADPKTGEHFGTPRRLNTIYRTNMQQAYSAARYQRYMDNVDNRPYWQYSAVGDQRTR
                     PAHQALNGKIYRYDDPFWATFYPPNGFNCRCTVIALGERDLKRKGIDEVGSSEPFLVK
                     AKRPKDKLGNQEETIGFKLPDGTVRLADKGFDYNVGRLTYKPNLDLYPEKLAHQFAKA
                     EMNGGEFKQAFKKLEDAVNQAKGSHKKLSADEMVKVRNALNQNFKFAAGRLSEETQKQ
                     IDSKVGTVWLSDDTLIKQFNSREGQNFDVSDYANLPDIFNNPLKVELDERDGWRFYAE
                     LNGKRYFAVIKVLAQYNEIFVQSFRLVSDKQWAKVFN"
     gene            4077..4493
                     /locus_tag="PWX02_000653"
     CDS             4077..4493
                     /locus_tag="PWX02_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HDL1728691.1"
                     /translation="MIEIQINGIDEIIATLNRIATKTKDKTDLMRNIAGTMESAVLTN
                     FDEGGRPKWLGIKHRQGTPLVDTENLMGSITADYNNDSAIVGTNEPYAAIHQFGGMAG
                     RGRKVKIEARPFLQLTSQDRQDILDDVQAYWQKLLD"
     gene            complement(4490..4708)
                     /locus_tag="PWX02_000654"
     CDS             complement(4490..4708)
                     /locus_tag="PWX02_000654"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728692.1"
                     /translation="MRKKSNGEQLPQVANFQKKVTAYIKQEMQPILKRFKSVLKNFKK
                     VGMINRTIKNKNATESDLGAICGDYLIA"
     gene            4752..5819
                     /locus_tag="PWX02_000655"
     CDS             4752..5819
                     /locus_tag="PWX02_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HDL1728693.1"
                     /translation="MFKQKFKLNPIALSFKLNQEANGRIQLCPFGWFEAQDGRGGRWY
                     VGDENGYALADDINNTAIDLMIDYEHQTLYIAENGKGNPAAGWITKAEYISGEGLFAD
                     VKWTPQATQEIKDGVYRYLSPLFLTDGSGMVIKVLNAALTNRPALHNLQEVVAMSSRF
                     APFLTPKEENPKMKELLIKLFALSANATDEQITEKLTALEKAKGDSPVALSDVYAELA
                     KEKGNVVALSAKVNNPDPAKFVALSDLQAVQTELNGLKQQINDKERDALIQTALSDGR
                     LLPAQKEWAENLGKQNLVALSDFLKTVTPNPALAGTQSGGEDPNKGAEKVALSAAEIA
                     TAKTLGLTPDEFAQKYKEEAK"
     gene            5819..6736
                     /locus_tag="PWX02_000656"
     CDS             5819..6736
                     /locus_tag="PWX02_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HDL1728694.1"
                     /translation="MFKKSELLKALDTAFKKEFGAGLALIKPQWEMVAMKISSSTKTN
                     TYGWLGQFPKMREWVGKRQIKKMQAEAMSIDNKTFESTVSVARTDIEDDQVGLFTPIV
                     QQAGQSAAELPDDLVFGLLAKGKETLCYDGQNFFDTDHPVYENVDGTGTSKTQSNITT
                     GAASGKPAFYILDDTNAIKPLIWQERTKPEIETKFDPSKSDTVFMEDEYLWGVRARGN
                     AGFGFWQLIHRVENSELNAETVMAVIAKMRMLKGDGGKLLNIRPSTILVPPTLEYAAR
                     QLVEADIINGTSNPLKGVLKVAVSAQIAE"
     gene            6782..7099
                     /locus_tag="PWX02_000657"
     CDS             6782..7099
                     /locus_tag="PWX02_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728695.1"
                     /translation="MMSRKKKDDETLNPKENTPPEDKVEEQPKPPEEETKPDDGAVID
                     PIAFEIKLKSIHPQATYGRCGYRFNKETAVEIARADLTDEQIITLANDPYLEFVPVVE
                     GDA"
     gene            7099..7524
                     /locus_tag="PWX02_000658"
     CDS             7099..7524
                     /locus_tag="PWX02_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 domain-containing protein"
                     /protein_id="HDL1728696.1"
                     /translation="MYALVDDFVLRIGEQESIELTDREMVGVVDEATLEVALADSSSQ
                     IDGYLSGRYRLPLKTVPQNLKRICCDLARYHLTSKSLVTMTEEVENRYKLCLKELENI
                     SKGVVSLAVDETNEEESATGENTVQFFNGGNRIWGRDKR"
     gene            7521..8162
                     /locus_tag="PWX02_000659"
     CDS             7521..8162
                     /locus_tag="PWX02_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HDL1728697.1"
                     /translation="MISKIERALIERLTLGLGKMVYAVAGYAGEINDEKLDVLRLPAC
                     LVSYGGSAFEAKSMGVRGKRYQATDVFVVLVITRSMRNQTAGRLGGVTSREVGVNQLL
                     GAVKTLLINQTLDGLVHPIQPKRIRTIWNNAEVKKEKLSAYAIEFEISYNECNVLDDG
                     RFPEGVEPNEQVFKQYQGKLDEPQGDLSGFNNRIFDPNNNAETAFRTEFKDGD"
     gene            8163..8342
                     /locus_tag="PWX02_000660"
     CDS             8163..8342
                     /locus_tag="PWX02_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HDL1728698.1"
                     /translation="MLKVKAAKGVRVPVENRPHHYIEQEAVEVENTLYYQRRIADGDL
                     IVVKETKSAKETGGK"
     gene            8342..9751
                     /locus_tag="PWX02_000661"
     CDS             8342..9751
                     /locus_tag="PWX02_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDL1728699.1"
                     /translation="MSKIEFEKIPNSIRKPSVLTEYNNKDAVTTLPTNEQEVLIVAPM
                     LNGEAAFTAPQKIFSDVEAENLFGKGSVAHLMVRQAIQNNPLIRLTVVGLKDHEAGIA
                     ATGQVSFTGTVTYAGVVRITIAGTAYEIAAAKGEEAQAIVARLVNVINAASYSPVVAS
                     VESETTLKLTSKAKGEIGNEITLATRNTATGLTLEARAFDNGQRNALIAPALASVAGT
                     HYNVIISPFSDDENALALRSHLESVSAPIEDKPAIGVMGWRGTYATGTTLTASLNSER
                     IIVGWYKGATESNAMIAAGLGAVIAGEEDPARPLNTLEVKGLTVVDDSQKPLFSEVNQ
                     ALYHGLSPIEVVASRVQITRAITTYTKNVTNTDDPSYLDLTTIRTLDYVRKAIQTRQR
                     LRFPRAKNSHRIVAKVRSEILDVLYQLESEEVIENVEAWKNRLLVVRNQDPTYLDLEI
                     PADVVNGLHVIRNKITLIL"
     gene            9762..10136
                     /locus_tag="PWX02_000662"
     CDS             9762..10136
                     /locus_tag="PWX02_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL1728700.1"
                     /translation="MSIVYEGSCVLEVDGVEIEITKLDVKTVTGRKLVKTMNSSGRAR
                     GYSQGIAEYTLSVTAVEPQDGTVIDWDNIKNAKLTKYPLDNSEKRTSYLGCFTTDVSE
                     SYTVDGESVRDIQLNALRKVNE"
     gene            10136..10504
                     /locus_tag="PWX02_000663"
     CDS             10136..10504
                     /locus_tag="PWX02_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728701.1"
                     /translation="MKITLNFGIEYNGKIRRDCTVRLLTMGGQCTAQEMIADLGFEEN
                     SEALSKREQTLVDMAYLSQQVTFDGIYQGNVTAQFLFDNLAPEDYWQLIEATLTLRKK
                     RLENGVSQSESESPANPDLA"
     gene            10534..10722
                     /locus_tag="PWX02_000664"
     CDS             10534..10722
                     /locus_tag="PWX02_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728702.1"
                     /translation="MILLSKFAITAEQVWQMPLLEVFAWIESYLESEGVKTPHSPMGA
                     NSEVKRESYVFTRCGKIE"
     gene            10775..13054
                     /locus_tag="PWX02_000665"
     CDS             10775..13054
                     /locus_tag="PWX02_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL1728703.1"
                     /translation="MAKNMKVQLELTAKDTASPVLNQAAKTTEKAFKNTERAAQQSGN
                     AQTQAAQKAANATESSNRKIEQSYRNARRVAADVSRARETLGVRAENAIQREIEQTRA
                     AYDRLKRSGVASQNELRRASEATKKRVQELNAELGKTSFGDKAGSFGRGLMGAGVGIA
                     AGAAMARQPARNQMEFDRQLAMVANTAFSDRDVKGRIDGKRELLDAVQKAVSVGGGSK
                     EEALSSLDALLASGAVSTETALNLLPTLQKGAVATGASANDMAQIAISSMQQFGIKEE
                     DIGRVLDMAVAAGQAGSFELANMASWLPQQMAAAKQAGLSGIEGFERLLIANQQARVT
                     AGTSNEAGNNLVNLLGKITAKETNERFKNIEYKDPKTGKTKAIDFAKAMEHYKGKGQD
                     SLQAFMSIMDDVVGSDKRYQELQAKLKTAKGEDQKRLFNELTNLVEGTAIGEIISDRQ
                     ALMALLGIKNNVQLGEKVQKDIQNSEGAVETSHAVIRDTNSHKVEALKNATEFAQMEN
                     FEKVNGVLGTLAEHLTSYANQYPNLTQFVSGATDAVKIFGAALAASSVLDLLTGKKGN
                     DGGMGDLFDKSSKATGSKGKVSKFLSVGGSFASVAGVATMLHGDQAPMSEERKAQLND
                     PNYQARQMYEYKKEILGSRFHSFFRSNETLEQYRKDVSAYEAKQKNGNTMGAVQGVLS
                     TIPSLQELRPKVDSVTQTLSTYQADFQAFGATLSAGIEAGLASQSHTIANQITVELDG
                     SVVAEKVAEYQFNFNKRMA"
     gene            13054..14346
                     /locus_tag="PWX02_000666"
     CDS             13054..14346
                     /locus_tag="PWX02_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HDL1728704.1"
                     /translation="MSGWTMPIQQASFKGVRFDVLAVDDSFERAVIEHAYPFVNGADL
                     EDMGLNTQTIRLQAVFFGSSYYTDYIRLLSVLQQKGADVLVHPIRGRLPNMLLVSANL
                     HTDAENINYVALDLTFKEATEMQPIFAIEDSLITKIDRLLLDLENLFDDGLRFWDSVA
                     VFDLKSRLLGLWGALFASFEAVRNLFDLDKKKYSIASSTSQRTYLKDGSGALHQLKEM
                     VDSGIQGRADLTTLSFKSQLLNITDAVDNIQAIVSKVARDEGFNRSKTVKLLGSDVVE
                     LGIMLDLIATTKLTKVLTELIEDREEELLIGDLELINQIVREHWLNIINKLREQQKHA
                     QKGIMSDKTKSIYETAEKLINRIRNVAHQFTVLVLAVINKKPPLTVRMVMDSGTIHKV
                     AHYFYGDYERADELLRLNPHIRQPNFIERGTLLNCYWE"
     gene            14349..15476
                     /locus_tag="PWX02_000667"
     CDS             14349..15476
                     /locus_tag="PWX02_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL1728705.1"
                     /translation="MENEIVVEIDGSQHKNWKSYDIDSDFLIPADSFNFELGKSSNIE
                     VLPNYSGKTAIVKINGKTVLTGIVDTTHHTISKSGRGFSLNGRDKAAILVDCSAPITN
                     VQGLSLLDAVKKIVTPLGIKEVQLKAEKNPTLSKVDIDVGESAWEAIMRCANSAGLHC
                     WFDPKGVLIVGGADYAQPPVATLYCVKESGDSRKNNFVDATLSYDVSQSYSEVTFLAQ
                     KHGKDSDNAKHDFKWVYKNEEMELYKPKTIVLGDVENLEALMKQAKKQVSDWQLESFN
                     LTIIVPDHKTKDGTLWQAGQRVHVVCEEYDLDGIFFLMGRRFRLSRTGGTTTELRLKL
                     DGVWTPDAYQAKAQEARKRKGKKGRKKKEPVEYVGSWELGK"
     gene            15481..16128
                     /locus_tag="PWX02_000668"
     CDS             15481..16128
                     /locus_tag="PWX02_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005711896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HDL1728706.1"
                     /translation="MRKLAKQTKEVAKGVQDSVRAAFRGVLNLVKSGSDIQQVQVSAL
                     ADETIQDLELMQHFGFTSVPPAGTQAVVIPLGGKTTHGIIVATENGSFRVKNLNDGEV
                     AIYDSSGSTIILKNGRVIDIECDSFNIKCKQYSVTASGGANFNTPKLETDQQFVSQGQ
                     ISGNGGMAVQGGEGASFSGNVNQTSGSFKTSGDVTAGTVSLKNHTHQSDGRGKPN"
     gene            16237..16587
                     /locus_tag="PWX02_000669"
     CDS             16237..16587
                     /locus_tag="PWX02_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HDL1728707.1"
                     /translation="MDREISPLTRDYTSDNIATLQNAVYIRLTTPKGSWWADGRVGSL
                     LHLIQREKDLSRVGLLAQQYAEEALQPIIDDGRAKSITVTHEQPKDGSLNLLISVIDN
                     RGVKFEFKHPVKLV"
     gene            16600..17661
                     /locus_tag="PWX02_000670"
     CDS             16600..17661
                     /locus_tag="PWX02_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDL1728708.1"
                     /translation="MYQIPTLEDVRREILRDIQSLEPTADIEVDSDYFVRASALASCV
                     VGLYAHQNWIVRQYFPDTADSEYLEMHAKLRNLYRKNATYASGSLKVFGTAGSVINEG
                     LQVKFGERFYVTKSSDVIGDDGSATVRVISMATGASSNVKSESKAQLTAAPAGVSSDC
                     LLLPDVTGGTEVESDATLLARLLEQIRRPPSGGNKNDFRQWAESVDGVTSAFVYPLRR
                     GEGTVDIAITSGDTVPSDEIVRATQAYIDEVRPVTAKSVYVLKPVEKRVNFNIKVQLD
                     TDTTLDGVKAEIENALENYFLSLKPADTLIISQIEAVISDLVGVVDREIVQPTRNQTI
                     DAYREMGWFRLGDVTVELM"
     gene            17661..18227
                     /locus_tag="PWX02_000671"
     CDS             17661..18227
                     /locus_tag="PWX02_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HDL1728709.1"
                     /translation="MVTINSKHGKALGGLLPPVSYDPNGRWLALSLEVEGRELDRIDA
                     RAKTLIDAVDVSTGIFIDDWERVCGLTANSSLLLETRIERVIAKLNQLGGLSISYLME
                     QAKALGYEFKIHEPEPFRCGINRCGDRLWDKDVIWMFFVDFNRAENTDPYLPAPVIKT
                     PQALKLLLDEIKPAFTRYWVRDFTAWNN"
     gene            18228..>20794
                     /locus_tag="PWX02_000672"
     CDS             18228..>20794
                     /locus_tag="PWX02_000672"
                     /inference="COORDINATES: protein motif:HMM:NF023638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728710.1"
                     /translation="MRQTMNPITTDNGRFKDGNPATGEYGTVVTAEHMNNVQDSVISM
                     QNEIITVLNEAGIAVNPNDNTQLWQALQMIAGQVESIADLRQFEPVKDRQVVFVKAYH
                     TGSDKGGGYFVSDFADTVSADNSVTLFVTANGKRWKRLYSTLNIFDFGVRLDSVNNDA
                     INRLQAWQEPVLGLGNVIPASSRIVPASHISQVAYRFNGIDYLSEDYYKADLSQITRT
                     GYYTAWTQDKAFVHNGVIYAPFMLAFRHGYDDLRIAWVKSFDNGNTWTTPEILMDYHP
                     QNPTLGWHCFSMGIVGNRLFMLVEERDVASKKMVRSFLYSRVMTKNFFVTGGISQSGN
                     VVTVELEEHGLFVGDSISFSNSGISGVSGKMTVTSVLDANRFTVMNSKEQTITNNDSW
                     LCATYFEDNQWQIQELSTFANSAGVAATHLHSFCPVNRSQFAFGFHNGDASPREIGFV
                     KCSINWATGRVTTDKIILSETLSRAAAEPCLRYYKNKLFLTTRSQNPNVGSLLIRCDL
                     NGENISSFNISTEKIHYTNMPFHIENDVIYMFAAERAENEWETGQSDKSGNRYRANRP
                     RMFLLKHRLSDWGKPEKTEVQVISQGIYSGEAANSACGVGSVVYKDGVLYCLFGDEDY
                     RNSHSLTENARRANNAHIDSGYQPEIYALRIVLDKTLSGLTDRPLKGADNLHLPVFRG
                     VNGVRTIQSPIEFDETTLFNKLASFVSMQVQKILATGDDYGMYGTDYLNGVNRLYLSS
                     THKANSQNGSTITLHNDKSEEGSIIDYRATAHKFLGGVHINTGVINTPPESAGNTEIV
                     NAKWVNSQIEKNNTEKVLFSGSTQEPVSVQVSSLRGEIHVLASIRGVKRWDSFSLWS"
BASE COUNT         6057 a         4002 c         5257 g         5478 t
ORIGIN      
        1 tcggcacaat tctacattat gacagcgtac ttaaccgagt tttaagcacc aaaggctgga
       61 aaaaagccca ttttaaagcg attttacgct tccccgacaa tatggcgtta tgggacgaat
      121 gggagaatat ctatctttct gaagagggcg atgatgacac gctttcagac ctattctatc
      181 agcaacataa agctgagatg gataaagggg ctgtcgtttc gtggttggct cgccctattc
      241 tctatttaat gaaaattcgt gctagtgatg ggcatagttc ttttgactcc gagtatcaaa
      301 acgaccctgt ttcgggtgat gatgcgattt ttgccaatag tattcaatac tggacggagt
      361 tgcccgaaaa cttaatctat tttggggcat tagacccttc aatgggtaag gctggagcaa
      421 gccgagaccc atctgcaatt ttggtgggcg gttatcaccg tgagagcggt aagctctatg
      481 tggttgaggc tcaaattaaa aaacgattgc cggatttaat tattgaggac gtgattagac
      541 tgcattcgca atataactgt caccgctggt ttgtggagac ggtgcaattt caggagtttt
      601 tgcagacgga actggtcaag cgttctgccc agcgtggtaa gccggtgccg gcaacggcaa
      661 ccaagccgaa cagtgacaag atgttacgga ttgagagctt gcaaccgcat attgccaacg
      721 gtttgatttt gttgcaccgc tcgcaatcta cgcttgaaag ccagcttcgc cattttccga
      781 aagccgatca cgatgatggt ccggatgcgt tggaaatgtt atggcgaaat gcactcagca
      841 gctctgcccc gattgagtgg gagagtgtag atgattttga cggtgagtat cgcagtaagt
      901 ggcggcatta atgtaactcc cctctttgct aaagaggggg actatattga gcgtaactaa
      961 gctctggaaa ggaaaattta tgagcaaaaa gaagcataac aagcagatta aaccgaattt
     1021 aaacccgatt aaaaatcaag aattgcagac ggatttagcc gaaattaccg ctacaggtcg
     1081 ggtactctcc gaccatccga gcaattttat tacccctgct aaaatgaaag cgatttttga
     1141 agatgcggag aacggcgata ttaccgccca gcacgagctg tttatggata ttgaagaacg
     1201 ggacagtacg atttttgcca atatccaaac ccgtaagcgt gccgcattag gcgtagattg
     1261 ggcgattgtt gcccctcgca atgccacacc gcaggaagaa aaactgcgtg atgaggttga
     1321 tgagttattt tatcagctcg gcaaccttga agattttgtg atggactgta tggacgctgt
     1381 gggacacggt tttgctgccc tcgaaattga atgggcgttt aacggtaaat tgtggtatcc
     1441 gaacgccttt atccaccgtc ctcaatcgtg gtttaagtgg gataaggtag ataatctgtt
     1501 gctgaaaacc cgacaaaata ccgaaggtga gccattacga gaatttggct gggtggtgca
     1561 tacgcataaa tcccgctcca cacaggcggc acgcaataat ttattccgct cgctggcttg
     1621 gctgtatatg tttaaacact actctatcca cgactttgcc gagtttttgg agctgtatgg
     1681 tatgcctatc cgcattggta aatacggtgc aggggcaaca ccaaacgaaa aacaaacgct
     1741 taaacgtgca ttagcggaaa tcggacacaa cgctgccgga attatgccgg agtcgatgag
     1801 tattgagttg cataacgccg ctaatgcagg cggtgcagcc ggcaataatc cgtttttgca
     1861 aatggtggat tggtgtgaga agtctatcgc ccgcttgatt ttggggcaaa cattaaccag
     1921 cggtgcagac ggcaaaagct ctaccaatgc actcggcaat gtgcataacg aagtgcggcg
     1981 tgatttgttg gtgtcggacg tgaaacagct ggggcagacg tttacccaac aaattattct
     2041 gccgtttctg caaattaact tcggcaatat cgacccgacc cgcattccga gttttgagtt
     2101 tgataccaaa gagcctgccg acttggcgtt atttgccgag agtttgccaa aattggtgga
     2161 tattggctta ccgattccga cccaatgggc gatggataaa ttaggtattc cggaggctca
     2221 agagggcgaa gcggtgctgg ggcgtgtcgc tcctctgcca acatcggggc aagcggtggg
     2281 attatcggca cattttgtta aatctccccc taaccccctc tttgtcaaag agggggaaca
     2341 gattactggc tgttcttgtg gttgtggggg taaggttcag gccttgtcgg ctcaacccca
     2401 aaccggtatt aaagagcaag atgcgttaga tagcttagcc aatgaagcct ttgccgtgcc
     2461 ggattttaac aagcagttcg accccatcac gaaaaaggcg gtggcggtgg taatggcgtg
     2521 tagctcttat gatgaggcag cagagaaact agcagcagcc taccccgact tagtgagtga
     2581 agaacacgaa cgctacttag ccaatgcgtt atttttagcg gatttattag gggctgcgaa
     2641 tgcagaaagt taattttgcc attggactag agcctaagca ggcgattgag tttttacggc
     2701 agaagaagtt gcttgcgaat aaagtgttta agcaggaatt atttgattct gcattggggc
     2761 gagccaccac cattagcaag ctcactagcc ttgaaatgac ccgtgatatt tatgagtcga
     2821 tggaaaaagc acggcgtgag gggaaatcct ttaatgaatg gaagaaaacg cttacggcag
     2881 aatttgagcg gaaaggctgg gtgtatggac acgacaaggc gattagtcgt ggtattgatg
     2941 gcaaattact tgccgacccg aaaactggcg agcattttgg cacgcctcgc cgcttgaata
     3001 cgatttatcg taccaatatg cagcaggctt attctgcggc tcgttatcag cggtatatgg
     3061 ataatgtgga taatcgccct tattggcaat attcggcggt aggcgaccaa cgcacccgcc
     3121 ctgctcatca agcgttaaac ggcaaaattt atcgttatga cgatccgttt tgggcgacct
     3181 tttacccacc taacggcttt aattgccgtt gcacggtgat tgcactgggt gagcgtgatt
     3241 taaaacgcaa agggattgat gaggtcggta gtagtgagcc gttcttagtt aaggcaaaac
     3301 gcccgaaaga taagctcggc aaccaagaag aaaccattgg ttttaaactg cctgacggca
     3361 cggtgcgatt ggctgataaa gggtttgatt ataacgtggg gcgtttaacc tataagccga
     3421 atttggattt ataccctgaa aagctggctc accaatttgc taaggcggaa atgaatgggg
     3481 gtgagtttaa gcaggctttt aagaagctag aagatgcggt aaatcaagcc aaaggcagcc
     3541 ataaaaaact ctccgccgat gagatggtta aggtgcgaaa tgccctcaat cagaacttta
     3601 aatttgcggc aggtcggtta agtgaggaaa ctcaaaagca gattgatagc aaagtgggta
     3661 ctgtatggct ttctgatgat acgctgatta agcaattcaa tagccgtgag gggcaaaatt
     3721 ttgatgtatc ggattatgca aatttacccg atatttttaa taacccatta aaagtagaat
     3781 tagatgagcg tgatgggtgg cgtttttatg cggaattgaa cggtaagcgt tattttgcag
     3841 tgataaaagt gttggcccaa tataatgaga tatttgtgca gtcgtttaga ttagtaagcg
     3901 ataagcagtg ggcaaaggtt tttaattagc cgctaggtag ggctcgaacc acctacacac
     3961 agtccagagt ctgttgcacc tcgtcgcttg cgatcctcga gattatcatc gcttttctag
     4021 cggctgttta aaatataccc ctgtttattt taaaaatcaa ctcagatggt taaaaaatga
     4081 ttgaaatcca aattaacggt atagatgaga ttattgccac tctcaaccgt attgcgacta
     4141 aaaccaaaga taaaactgat ttaatgcgga atatcgccgg tacgatggaa tcggcagtgc
     4201 tgacgaattt tgatgagggc ggtcgcccga aatggctggg tataaaacat cggcaaggca
     4261 cgccgttagt tgatacggaa aatttaatgg gcagcattac tgctgattat aataatgatt
     4321 cggcgattgt tggcacaaat gagccttatg ctgctattca tcaattcggt ggtatggctg
     4381 ggcgtgggcg taaagtaaaa attgaagccc gacctttttt acaattaacc tcgcaagaca
     4441 ggcaagatat tttggacgat gtgcaagcct actggcaaaa actgctggat taagcgataa
     4501 gataatcgcc acagatagcc cctaaatcgc tctctgtggc gtttttgttt tttatggtac
     4561 gatttatcat tccaactttt ttaaaatttt ttaaaacgga tttaaagcgt tttaaaatgg
     4621 gttgcatttc ttgcttaata taagcggtca cttttttctg aaaatttgca acttgtggaa
     4681 gttgctcccc gttactcttt tttctcaatc ttctattctt tagcctcatt aatttgtttt
     4741 gaggtgtttt tgtgtttaag cagaaattca aactaaaccc cattgcattg agttttaaac
     4801 tcaaccaaga ggcaaatgga cgtattcaac tttgcccttt tggttggttt gaggctcaag
     4861 acgggcgtgg aggacgttgg tatgtaggtg acgagaatgg ctatgcgtta gccgatgata
     4921 ttaacaacac tgcgattgat ctgatgattg attatgagca tcagaccctg tacatcgccg
     4981 agaacggcaa aggcaaccct gctgcgggtt ggattaccaa ggcggaatat atctcgggcg
     5041 aaggcttgtt tgcggatgtt aagtggacac cacaagcgac ccaagagatt aaagacggcg
     5101 tttatcgcta tctctcgcca ctgtttttga cagacggcag cggaatggta attaaggtac
     5161 ttaatgccgc cttaactaac cgccctgcct tgcataatct gcaagaggtg gttgcgatgt
     5221 ctagccggtt tgccccattt ttaaccccaa aagaggaaaa tccaaaaatg aaagaactct
     5281 taattaaatt atttgcattg tcggcaaatg cgaccgatga gcagattacc gaaaaactga
     5341 ccgcacttga gaaagccaaa ggcgacagcc cggtggcgtt gagcgatgtg tatgccgagc
     5401 ttgccaaaga gaaaggcaat gttgtggcgt tatcagccaa agtgaataac cctgacccgg
     5461 cgaagtttgt ggcgttaagc gacttgcaag cagtgcaaac cgagctgaac ggcttaaaac
     5521 agcaaatcaa cgataaagag cgtgatgcgt taattcaaac tgctctctct gatggtcgtt
     5581 tattaccagc tcaaaaagag tgggcggaaa acttaggtaa gcagaattta gtggcgttat
     5641 ccgacttttt gaaaacggta acacctaacc ctgctttagc aggtacgcag tcggggggtg
     5701 aagacccgaa caaaggtgcg gagaaagtgg cgttatcggc tgcggaaatt gcaaccgcta
     5761 agacgttggg cttaacccca gatgaatttg ctcaaaaata caaagaggaa gcgaagtaat
     5821 gtttaaaaaa tcggaattat taaaagcctt agatacggcg tttaaaaaag aatttggtgc
     5881 aggtttagct ttaattaaac cgcaatggga aatggtggcg atgaagatta gttcttcgac
     5941 caaaaccaac acttacggct ggttgggtca attcccgaaa atgcgtgagt gggtgggcaa
     6001 acgccaaatc aagaaaatgc aagcggaagc gatgtcgatt gacaataaaa cctttgaatc
     6061 gacagtcagc gtggctcgta ccgatattga agacgatcag gtcgggttat tcaccccgat
     6121 tgttcaacaa gccggtcaaa gtgcggcgga attgccggat gacttggtat ttggcttgtt
     6181 ggcaaaaggt aaagaaacgc tttgctatga cggtcagaac tttttcgaca ctgatcaccc
     6241 tgtttatgaa aacgtggacg gcactggcac gagcaaaact caaagcaata tcaccaccgg
     6301 tgcagcaagt ggcaagcctg cgttttatat tttagacgat accaatgcga ttaagccgtt
     6361 aatttggcag gaacgcacta agcctgaaat tgaaaccaag tttgacccgt caaaatccga
     6421 tactgtcttt atggaagatg aatatctgtg gggggtgcgt gctcgtggta atgccggctt
     6481 tggtttctgg cagttaatcc accgcgtaga aaattcagag ctgaatgcgg aaaccgtaat
     6541 ggctgttatc gctaaaatgc gtatgctcaa aggcgatggt ggcaagttgc tgaacatccg
     6601 accaagcacg attttagtgc cgccaacatt ggaatatgca gctcgccagt tagttgaggc
     6661 ggatattatt aacggcacaa gcaacccatt aaaaggtgtg ctgaaagtag ctgtttctgc
     6721 tcagattgca gaatagtaag ttaatcgggc gtagcaatac gccccttttg agaggatagc
     6781 aattatgagt cgtaagaaaa aagacgatga aaccttaaac ccaaaggaaa atacaccacc
     6841 tgaagataag gttgaagagc aaccaaaacc gcctgaagag gaaacaaaac ctgatgatgg
     6901 tgctgtgatt gaccctatcg cctttgaaat caagctgaaa tctatccacc cacaggcaac
     6961 atatggtcgt tgtggctacc gttttaataa agaaacggcg gtggaaattg ctcgtgctga
     7021 tttaactgat gagcagatta tcacattagc taacgaccct tatttggagt ttgtacctgt
     7081 tgttgagggc gatgcgtaat gtatgcgttg gttgatgatt tcgttttgcg aattggcgaa
     7141 caggaatcca ttgagctaac cgaccgtgaa atggtaggcg tggttgatga agcgactctt
     7201 gaagtagctt tagcggactc ttccagccaa attgatggct atttaagtgg gcgttaccgt
     7261 ctgccattaa agactgtacc gcaaaattta aagcgtattt gctgtgattt ggctcgttat
     7321 catttaacca gcaaatcatt agtaacgatg accgaagagg tggaaaaccg ctacaaactt
     7381 tgcttaaaag agctggaaaa catctctaag ggcgtagtgt cgttagcggt tgatgaaacc
     7441 aatgaggaag aatcggctac tggcgaaaat acagtgcagt tttttaacgg gggcaaccgt
     7501 atttgggggc gtgataagcg atgattagca aaattgagcg tgctttgatt gagcgtttaa
     7561 cgctgggctt aggcaaaatg gtgtatgccg ttgctggtta tgccggggaa attaacgatg
     7621 agaagttaga tgttctccgt ttgcctgctt gtcttgtctc ttatggtggc tcggcgttcg
     7681 aggcgaaatc aatgggagta agaggtaaac gctatcaagc cactgatgtg tttgtggtgt
     7741 tggttattac tcgctcaatg cgaaatcaga ctgctgggcg tttaggtggg gtaacaagtc
     7801 gtgaggtggg ggttaatcag ttgctgggag cggtgaaaac cttattgatt aaccagactt
     7861 tagacggttt agtccaccct atccagccta agcgaatccg cacgatttgg aataatgcgg
     7921 aggtgaagaa agaaaaactc tctgcttatg ctattgagtt tgaaatcagc tacaacgagt
     7981 gcaatgtgct tgatgacggt agattccctg aaggggttga gccgaatgag caagtcttta
     8041 agcagtacca aggcaagcta gatgaaccgc aaggcgattt gtcgggcttt aataatcgga
     8101 tttttgaccc gaataacaat gccgaaacgg catttagaac agaatttaag gatggtgatt
     8161 agatgttaaa agtgaaagcc gctaaagggg tgcgtgtgcc ggtagaaaac cgaccgcatc
     8221 actatattga acaagaggca gtagaggttg aaaacaccct ttactatcaa cgccgcattg
     8281 cggatggcga tttgattgtc gtcaaagaaa ccaaatccgc taaagaaaca gggggcaaat
     8341 aatgtcaaaa attgaatttg agaaaatccc gaacagtatt cgcaaaccaa gtgtgctgac
     8401 cgaatacaac aacaaagacg cagtaaccac cttgccgacc aatgagcaag aggtgctaat
     8461 tgttgcacca atgctaaacg gtgaggctgc ttttactgct ccgcaaaaga tttttagcga
     8521 tgtggaggcg gaaaatttat ttggtaaagg ctcggtagct catttaatgg ttcgccaagc
     8581 aatccaaaat aatccgctaa tccgtttaac ggtggtgggc ttaaaagacc acgaggcagg
     8641 tattgcagca acagggcaag ttagttttac cggcacagta acttatgccg gtgtggtgcg
     8701 tattacgatt gccggcacag cttacgaaat tgcagccgca aaaggcgaag aagcacaggc
     8761 gattgtggct cgtttagtta atgtgattaa tgcggcgagt tatagccctg tggtggcaag
     8821 tgtggaaagt gaaacgacac ttaaactgac ctcaaaagca aaaggcgaaa tcggcaatga
     8881 aatcacgctt gcaacacgca atacagcaac aggtttaacc cttgaggctc gtgcgtttga
     8941 taacggtcag cgtaatgccc taattgctcc ggcgttggcg agtgttgccg gtacgcatta
     9001 taacgtgatt atttcgccgt tttcagatga tgaaaatgcc cttgccttgc gtagccattt
     9061 agaatcggtt tctgccccga ttgaagataa accggctatt ggtgtaatgg gctggcgtgg
     9121 cacttatgcc accggcacaa ccttaaccgc aagccttaac agtgagcgaa ttattgtagg
     9181 ctggtataag ggagcaaccg aatcaaatgc aatgattgcg gcagggcttg gtgcggtaat
     9241 tgccggtgaa gaagatccgg ctcgcccgtt aaataccctt gaagttaaag gcttaacggt
     9301 cgtagatgac tcgcagaaac cgttatttag cgaagtaaac caagcattat atcacggttt
     9361 aagcccgatt gaggtggtgg caagccgtgt gcagattacc cgtgcgatta ctacatacac
     9421 caaaaacgtg acgaatactg atgacccaag ctatttggat ttaaccacca ttcgtacgct
     9481 tgactatgtg cgtaaggcaa tccaaacccg tcagcgtttg cgtttccctc gtgcgaaaaa
     9541 ctcccaccgt attgtggcaa aagtacgctc ggagatttta gatgtgcttt atcagcttga
     9601 gagcgaagag gtaatcgaaa atgtggaagc gtggaaaaat cgcttattag tggtgcgtaa
     9661 tcaagacccg acttacttgg atttagaaat ccctgcggat gtggttaatg gcttgcacgt
     9721 tattcgtaac aaaattacgt taattttata gggggtgcta gatgtcaatt gtttatgaag
     9781 gctcttgcgt gcttgaggta gatggcgttg agattgaaat taccaaactt gatgtaaaaa
     9841 ccgtaaccgg tcgcaagctg gttaaaacga tgaacagttc cggacgtgca agaggttatt
     9901 cgcaaggcat tgcggaatat actctctcgg tgactgcagt tgagcctcaa gatgggacag
     9961 tgattgattg ggataatatc aaaaatgcta agctcactaa atatccactg gataacagtg
    10021 aaaaacgtac ctcttattta ggctgtttta ctaccgatgt gagcgagtct tacaccgttg
    10081 atggtgagtc ggtgcgtgat attcaactca acgcattgcg taaggtgaat gaataatgaa
    10141 aatcacttta aattttggca ttgaatataa cgggaaaatt cgccgtgatt gtacggtgcg
    10201 attgctgacg atgggcggtc aatgcacggc tcaagaaatg attgcggatt tagggtttga
    10261 ggaaaactcg gaagccctat caaagcgtga acaaaccttg gtcgatatgg cttacctttc
    10321 gcagcaagtt acctttgatg gtatttatca aggtaatgtg acggctcaat ttttatttga
    10381 caatttagct cccgaagact actggcagtt gattgaggca acgctcacac tccgaaaaaa
    10441 gcggttagaa aatggggtga gccaaagcga gagcgaaagc ccggcaaatc ccgatttggc
    10501 gtagcagaag cctttaagca gtaccgacaa gcggtgattt tactctcaaa atttgcaatt
    10561 accgccgagc aggtatggca aatgccgttg cttgaggtat ttgcgtggat tgagagctac
    10621 ttggagagtg agggcgtaaa aacaccgcat agcccaatgg gtgcgaatag cgaagtgaaa
    10681 cgcgaaagct atgtctttac tcggtgtggc aaaattgaat aggggcgaaa gccccttttt
    10741 tgtactttaa aattagttta aagagacttt aaaaatggca aaaaatatga aagtgcaact
    10801 ggaattgacg gcaaaagata ctgcctcccc agttttaaac caagccgcta aaaccaccga
    10861 aaaagccttt aaaaatacag agcgagcggc acaacaaagt ggcaatgctc aaacgcaagc
    10921 agcacagaaa gcagcgaatg ctacagaaag ctcaaatcgg aaaatagaac agagttaccg
    10981 taatgcccgc cgagtggctg ccgatgtgag ccgagcccgt gaaacattag gtgttcgggc
    11041 ggaaaatgca atccaacgtg aaattgaaca aactcgtgca gcctatgacc gcttaaaacg
    11101 ctcaggtgtt gcctcgcaaa atgagctacg ccgtgcttca gaggcaacaa agaaacgtgt
    11161 gcaggaattg aatgcggaac tggggaaaac gagttttggg gataaagctg gaagttttgg
    11221 gcgtgggttg atgggtgcag gggttggaat tgctgcaggt gcagcaatgg ctcgccagcc
    11281 tgcaagaaat caaatggaat ttgatagaca gcttgcaatg gtagcgaata ccgccttttc
    11341 agatcgtgat gtgaaagggc gtattgatgg gaaaagagag ttacttgatg ccgtacagaa
    11401 agcagtaagt gtaggcggtg gctcaaaaga agaagcgttg agctcactag acgctttact
    11461 tgcctcaggt gctgtttcta ccgaaaccgc tctcaattta ctcccaacgt tgcagaaagg
    11521 tgcggtcgca acaggggcaa gtgcaaatga tatggctcaa attgctattt cctcaatgca
    11581 acaatttggg attaaagaag aggatattgg tcgagtctta gatatggcag ttgctgctgg
    11641 gcaagccggt agctttgagc ttgcaaatat ggcaagttgg ttgcctcaac aaatggccgc
    11701 tgcaaaacaa gctggcttaa gcggcattga gggatttgag cgtcttttaa ttgccaatca
    11761 gcaagctcgt gtcactgccg gtacaagtaa cgaggctggt aataatttag tgaatctgtt
    11821 gggtaagatt actgctaaag aaactaatga gcgttttaag aatattgaat ataaagatcc
    11881 gaaaactggc aaaaccaaag cgattgattt tgcaaaagct atggagcatt acaagggaaa
    11941 agggcaagat tcattgcagg cttttatgtc tattatggat gatgttgtcg ggagtgataa
    12001 acgctatcaa gaattacaag ccaaactaaa aacagctaaa ggtgaagatc agaaacgctt
    12061 attcaatgaa ttaaccaact tggttgaagg tactgcaatc ggagaaatta tttctgatcg
    12121 tcaagcatta atggctttgc tggggataaa gaataatgtt cagttaggtg aaaaggtaca
    12181 aaaagatatt cagaatagtg aaggggctgt tgaaacctcc cacgctgtta tccgtgatac
    12241 taattcgcac aaagtggagg cattaaagaa tgcgaccgaa tttgcccaaa tggagaactt
    12301 tgagaaagta aacggtgtgt tgggtacttt agcggaacat ttaacgagct atgctaacca
    12361 ataccctaat ttaactcagt ttgttagcgg agcgactgat gccgttaaaa tctttggggc
    12421 tgcattagca gcctctagtg tgctggattt attgactggc aaaaagggaa atgatggtgg
    12481 aatgggtgat ttatttgata aatcatcgaa agctactgga agtaaaggaa aagttagtaa
    12541 atttctttca gttggaggct catttgctag tgttgcgggc gttgccacaa tgctacacgg
    12601 agaccaagct ccgatgagtg aggagcgaaa agcacaatta aacgacccta attatcaagc
    12661 tcgtcaaatg tacgaatata aaaaagagat tttaggtagc cgtttccact cgttttttcg
    12721 tagtaatgaa acccttgagc aatatcggaa agatgtgagt gcttatgagg ctaagcaaaa
    12781 gaatggcaat actatgggag ctgtacaagg ggttttaagt accattccga gcttacaaga
    12841 gttacgccct aaagttgatt cggttactca aaccctatcc acctaccaag ccgactttca
    12901 ggcattcgga gcaactcttt ctgccggtat tgaggcaggg cttgcctcgc aatctcatac
    12961 gattgcaaat caaatcaccg tggagttgga cggttcggtg gtggctgaaa aagtggctga
    13021 atatcagttt aattttaaca agaggatggc gtaatgagcg gttggacaat gcctatacag
    13081 caggcaagtt ttaaaggtgt gcgtttcgat gtgcttgcgg tagatgacag ctttgagcga
    13141 gcggtgattg aacacgccta cccttttgtg aatggggctg acttagagga tatggggcta
    13201 aacacccaaa ctattcggct gcaagcggtc tttttcggct caagttatta cacggactat
    13261 atccgcttac tgtcggtatt gcagcaaaaa ggggctgatg tattagtcca tcctattcgt
    13321 gggcgtttac cgaatatgtt actcgtttcg gcgaatctac ataccgatgc cgaaaatatc
    13381 aattatgttg ccttagattt gacttttaaa gaggcaaccg aaatgcagcc gatttttgca
    13441 atcgaagatt ctctgattac taaaattgat aggttgttgc ttgatctcga aaacctattt
    13501 gatgatggat tgcgtttttg ggatagcgtt gcggtgtttg atcttaaatc tcgcttgctt
    13561 gggctttggg gggcattatt tgcctcattt gaggcggtgc gtaacttgtt tgatttagac
    13621 aagaaaaaat acagcattgc ctcatcaacc tcacaacgaa cctatttgaa agatggttcg
    13681 ggggcattac accagttaaa agaaatggtg gatagcggta ttcaggggcg tgctgattta
    13741 acgacactct cgtttaaatc tcagttactc aatattacgg atgcggtaga taacattcag
    13801 gcgattgtga gcaaagtggc tagagatgag ggctttaacc gctcaaaaac ggttaagtta
    13861 cttggctctg atgtggttga gcttggtatt atgcttgacc ttattgccac gactaagctc
    13921 actaaagttt taacagaact gattgaggat cgagaggaag agctgctaat cggtgattta
    13981 gagttaatta accaaattgt gcgtgaacat tggttaaaca ttatcaataa actgcgtgag
    14041 caacaaaagc acgctcaaaa aggcataatg agtgataaga caaaatctat ctacgagacc
    14101 gcagaaaagt tgattaatcg tatccgtaat gtagctcatc aatttactgt acttgtgctt
    14161 gcggtaatta ataaaaaacc gcctttgacc gtgcgaatgg taatggatag cggcacaatc
    14221 cataaagtag cccattattt ttatggggat tacgagcggg cggatgagct gttacgcctt
    14281 aatccgcata ttcgccagcc gaactttatt gagcgaggca cgctgttaaa ttgttattgg
    14341 gagtaaaaat ggaaaatgaa attgtggtgg agattgacgg ctcgcagcat aaaaactgga
    14401 aaagttatga tattgacagc gattttttaa ttcctgctga tagttttaat tttgagttgg
    14461 gtaaatctag caatatagag gttttaccta attattcggg caaaacggca atagttaaaa
    14521 tcaatggcaa aacggtgctc actggcattg tggataccac acatcacacc attagcaaaa
    14581 gcgggcgtgg gtttagccta aatggacgtg ataaagccgc tattttggtg gattgctctg
    14641 ccccgattac caatgtgcaa gggctctctt tgcttgatgc tgttaaaaaa attgtaaccc
    14701 ctcttgggat taaagaggtg caactgaaag ccgagaaaaa cccgacctta agcaaggtag
    14761 atattgatgt gggcgagtca gcgtgggaag cgattatgcg gtgtgccaac tcggcaggtt
    14821 tgcattgttg gtttgacccc aaaggcgtgt taattgtcgg cggtgcggat tacgctcaac
    14881 cgcccgttgc cacgttatat tgcgtgaaag agtcggggga tagccgtaaa aataactttg
    14941 ttgatgcaac actttcttat gatgtgtcgc aaagctattc ggaggtcact tttcttgctc
    15001 aaaaacacgg caaagattct gataatgcca agcacgattt taaatgggtg tataaaaatg
    15061 aggaaatgga gctttataaa ccgaaaacta tcgtgctggg cgatgtggag aacctcgaag
    15121 ccttgatgaa acaggcgaaa aaacaggtat cagattggca gttggagtca tttaacctca
    15181 cgattattgt acctgaccat aaaaccaaag acggcacgtt gtggcaagct gggcaacgtg
    15241 tgcacgtggt atgtgaggag tatgatttag atggtatttt ctttttaatg gggcgacgtt
    15301 ttcgactctc tcgcacgggt ggcacaacca ccgaattgcg gttaaaactg gatggcgtgt
    15361 ggacacctga tgcttatcaa gctaaagcac aagaggctcg caagcgtaaa ggcaaaaaag
    15421 gacgtaagaa aaaagagcct gtagaatatg taggctcgtg ggagttggga aaataatatg
    15481 atgagaaaat tagcaaaaca gactaaagag gtggcaaaag gtgtgcaaga tagcgtgcgt
    15541 gctgctttta gaggggtatt aaatttagtc aagagtggca gtgatattca gcaggtacag
    15601 gtgtccgctt tagcggacga aactatccaa gatttagagc taatgcagca ttttggtttc
    15661 acttccgtgc cacctgccgg cacgcaagcg gttgttatcc ccttaggtgg taaaaccacg
    15721 cacggtatta ttgtggcgac tgaaaacgga tcgttccgag tcaaaaatct caacgatgga
    15781 gaggtggcaa tttacgattc gagtggctca accatcatat taaaaaacgg gcgagtgatt
    15841 gatattgagt gcgatagctt taacattaaa tgcaagcaat attccgttac cgcaagcggt
    15901 ggagcaaatt ttaacacccc gaaactagaa accgaccagc agtttgtttc acaagggcaa
    15961 atcagcggca atggcggaat ggcggtgcaa ggtggagaag gggcaagttt tagcggcaat
    16021 gtaaaccaaa ccagtggtag ctttaaaaca agtggggatg tgactgcagg tacggtatcc
    16081 cttaaaaacc atactcatca aagtgatggg cgaggtaaac caaactgatg atttcaagcg
    16141 gttatttttt gcaaaatttt tgcagaatct aaccgcttgt tgtttgtgga agtctctccc
    16201 cgttactcct gtctttaatg tctctaccat agcaatatgg acagagaaat cagcccgctc
    16261 actcgggact atacaagtga caacatagct acactgcaaa atgccgtgta tatcagacta
    16321 acaacgccaa aaggctcatg gtgggcagat gggcgtgtag gttctttgct ccatcttatt
    16381 cagcgagaaa aagatttaag ccgcgtaggg cttttagccc aacagtacgc cgaagaggct
    16441 ttgcagccga ttattgatga cggacgagcc aaaagcatta cggtgacaca cgagcagcca
    16501 aaagacggct cgttaaatct tttaatctcc gttattgata atcgtggggt taaatttgag
    16561 tttaaacacc ctgtaaaact ggtttaagtg gtttttaaaa tgtatcaaat cccaacgctt
    16621 gaagatgtta gaagagaaat tctacgagat attcaatctt tagagccaac tgctgatatt
    16681 gaggtggatt cggattattt tgttcgtgcc tccgctttgg caagctgtgt ggtggggctg
    16741 tatgctcacc aaaactggat tgttcgtcag tatttcccag atacggcaga ctccgaatac
    16801 ctcgaaatgc acgctaaatt gcgaaatctt taccgtaaaa atgcgaccta tgcgagtggt
    16861 tcgttgaaag tttttggtac tgctggctca gtaatcaatg agggcttgca agttaaattt
    16921 ggcgagcgtt tttatgtaac caaaagctcg gacgttattg gtgatgatgg ctctgcaacg
    16981 gtgcgagtga tttcaatggc aacaggggca tcaagcaatg ttaaatctga aagtaaagca
    17041 caactgactg ctgctcctgc tggcgtatca agcgattgct tgttattgcc tgatgtaaca
    17101 ggcggaacag aagtggaaag tgatgcgaca ctgcttgctc ggttattaga gcagattcgc
    17161 cgaccaccgt caggcgggaa taaaaatgac ttccgccaat gggcagaaag cgttgatggc
    17221 gtaacctctg cgtttgttta tccattacgg cgtggcgagg gtacggtaga tattgcaatc
    17281 acctcaggtg ataccgtgcc aagtgatgaa atcgtgcgag caactcaagc ttatattgat
    17341 gaagtccgcc ctgttacggc taaatcggtt tatgtactta agccagttga aaaacgggtc
    17401 aattttaaca tcaaagtgca acttgatacg gatacgacgc ttgatggtgt aaaagcggaa
    17461 atcgaaaacg ctttagagaa ttatttttta tcactcaaac ctgctgacac cttaattatt
    17521 tcgcaaattg aggcagtaat tagtgattta gtgggcgtag tagatcgtga gattgtacag
    17581 ccaaccagaa accaaacgat tgatgcctac cgtgaaatgg gctggttcag attgggtgat
    17641 gtaactgtgg agttgatgtg atggtaacga ttaactctaa acacggcaag gcattgggcg
    17701 gtttattacc gcctgtatcg tatgacccaa acggcagatg gctagcgtta tcccttgaag
    17761 ttgaggggag agagctagac cgcattgatg cgagagccaa gactttaatc gatgccgttg
    17821 atgtatcaac aggcattttt attgatgatt gggagcgggt ttgtggctta acggcgaata
    17881 gctcactact gcttgaaacc cgaattgagc gtgtgattgc gaagttgaat cagcttggtg
    17941 ggttgtcgat ttcgtactta atggagcaag cgaaagcact aggctacgaa tttaaaatcc
    18001 acgagcctga accctttaga tgcggtatta accgttgtgg cgatagactt tgggataaag
    18061 atgtgatttg gatgtttttt gtcgatttta accgagccga aaacaccgac ccttatttgc
    18121 ctgcacctgt gattaaaacg ccacaagcat taaaactatt attggacgaa attaaacctg
    18181 catttacccg ttattgggta agagatttta cagcgtggaa taactaaatg agacaaacga
    18241 tgaaccctat tacgactgat aacggtcgat ttaaagacgg taatcccgca acgggagagt
    18301 acggcacagt tgtaacagcc gaacatatga acaatgtgca agactccgtt atctcaatgc
    18361 agaatgagat tataactgta ttgaatgagg ctgggattgc agttaatcct aatgataata
    18421 ctcaactttg gcaggcattg cagatgattg ctgggcaagt tgagagtatt gctgatttac
    18481 gccaatttga gccagtaaaa gaccgccagg ttgtatttgt taaagcgtat catactggca
    18541 gcgataaagg tggtgggtat ttcgtttcgg attttgcaga tacagtttct gcagataata
    18601 gcgtaaccct atttgtgaca gcaaatggaa aacgctggaa acgactttat tcaacactca
    18661 atatctttga ctttggagtc agattagaca gcgtgaataa cgatgccatc aaccgcttgc
    18721 aagcgtggca agagccggtt ttagggttgg ggaatgtgat tccggcaagt tcaagaattg
    18781 tgccggctag ccatatctca caagtagctt atagatttaa cggtattgat tacttgtctg
    18841 aggattatta taaggcggac ctatcacaaa ttacccgaac aggttattac acagcttgga
    18901 ctcaagataa agcatttgtt cataatggag tgatttatgc cccctttatg ttggctttcc
    18961 gccacggcta tgatgacttg cgtattgctt gggtcaaatc ctttgataat ggcaatactt
    19021 ggacgacgcc agaaatttta atggactatc atccgcaaaa tccaacattg ggttggcact
    19081 gtttttctat gggcattgtt ggaaatcggt tatttatgct tgtagaagag cgtgatgtgg
    19141 cgagtaagaa aatggttcgg tcattcttgt atagccgagt aatgactaag aatttttttg
    19201 taacaggtgg catttcgcaa tccggtaatg tagtaaccgt agaattagaa gaacacgggt
    19261 tatttgtagg cgattccatt tcgttctcca atagcggaat ctccggtgtg agtggcaaga
    19321 tgacagtcac ctccgtatta gacgcaaacc gttttacggt gatgaatagc aaagaacaga
    19381 ctattaccaa caacgatagc tggctttgtg caacctactt tgaagataac caatggcaaa
    19441 ttcaagaatt aagcacgttt gccaatagtg ctggcgttgc cgctacgcac ttgcatagtt
    19501 tttgcccagt gaatcgttca cagttcgcct tcgggtttca taatggtgat gcttcacctc
    19561 gtgaaatcgg ctttgtgaaa tgtagcatca actgggcaac aggtagagtg acaacggata
    19621 agattattct ttctgaaacg ttaagtcggg ctgccgctga gccttgtttg cgatattaca
    19681 aaaacaaatt atttttaacg acccggtcac aaaatccgaa tgtagggtca cttctaattc
    19741 gttgtgactt aaacggggaa aatatcagtt cattcaatat ttccacggaa aagattcact
    19801 ataccaatat gccatttcat attgagaatg atgtgattta tatgttcgcc gccgaacgag
    19861 cggaaaatga atgggaaaca gggcaaagcg ataaaagtgg caaccgctat cgagcaaatc
    19921 gtccacgaat gtttttgctt aagcatagat taagcgattg gggcaaaccg gaaaaaacag
    19981 aagttcaggt tatttcgcaa ggcatttatt ccggcgaagc agctaacagt gcgtgcggtg
    20041 tggggtcggt tgtgtataaa gatggggtgc tttattgctt gttcggcgat gaggattacc
    20101 gcaacagcca ctcactcacg gaaaacgcta gaagagctaa caacgcccac attgatagcg
    20161 gctaccagcc agagatttat gctttacgta ttgtgttaga taaaacgcta tcaggtttaa
    20221 ctgatagacc gttaaaagga gcggataatt tacatttacc cgttttccgt ggcgtaaatg
    20281 gcgtgcggac aattcaaagc cccatagaat ttgacgaaac aacactcttt aacaagttag
    20341 cctcttttgt gtctatgcaa gtccaaaaaa tcttagctac aggcgatgat tatggtatgt
    20401 acggcacaga ttatctaaat ggcgtaaacc gcctttattt gtctagtaca cataaggcaa
    20461 atagtcagaa cggctcaaca atcacgctac acaatgataa aagcgaagag ggtagcatta
    20521 ttgactatag agcaactgcc cataaatttt tgggaggtgt gcatattaac acaggggtga
    20581 ttaatactcc gcccgaatca gcgggaaaca cagaaattgt caatgctaag tgggttaact
    20641 cgcaaatcga aaaaaataac accgaaaagg tgttatttag tggctctaca caagagcctg
    20701 tgtcggtgca ggtaagtagt ttgcgaggcg aaatccacgt gttggcaagt attagaggcg
    20761 taaaacgttg ggatagcttt agcctctgga gcag
//
LOCUS       DANJWC010000024        20510 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.024, whole genome shotgun sequence.
ACCESSION   DANJWC010000024 DANJWC010000000
VERSION     DANJWC010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20510
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.024"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            79..762
                     /locus_tag="PWX02_000673"
     CDS             79..762
                     /locus_tag="PWX02_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2572 family protein"
                     /protein_id="HDL1728711.1"
                     /translation="MNKYKNASALLVSLILISTILSVLFLSKEKWLGQQNITHFYREK
                     YLSDQYNFLALYPQDKTQICQKFQKEQISQEDLSDKQFRFLQYQFACQFHSLFKDKKP
                     TKEKYIHFTRLADYLDLSNVPNQEIYTIHSLAELPSSSEREPKIVLAKNAINENLPQD
                     FYGIVITDYLFDITGKKMYGTLYSSYDNAREERNLTFKKEVLAELEARYSAWRYLPYS
                     EHLMGAVDD"
     gene            755..1048
                     /locus_tag="PWX02_000674"
     CDS             755..1048
                     /locus_tag="PWX02_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5374 domain-containing protein"
                     /protein_id="HDL1728712.1"
                     /translation="MIKYKAETALSLLVAITLFAIVVLSFSQWQSAQNHRLNQHFQQQ
                     QAVMILENQLALKLAGLDCESSITQNQIRYQIQCSGNRLTIRFPLGKIELNND"
     gene            1097..4387
                     /gene="recC"
                     /locus_tag="PWX02_000675"
     CDS             1097..4387
                     /gene="recC"
                     /locus_tag="PWX02_000675"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HDL1728713.1"
                     /translation="MFTLYQSNQISSLAEMLVKIQQVNPLEDPFEPETILIQSQGMAQ
                     WLQMQIAELNGVMGNCDFLYPTTFLWQQYRLLFPELPKENIFERSSLVWRIMRLLPSC
                     LTLSEFAPLKRYLGDQPNKEQYQLKLYQLSAKIAELFDQYLVYRPHWLVSWEEGNLQA
                     IENEIKIATAAKPIDFDLIRADMKWQSHLWQQIVSDLQSESDEQVFTTSHRAYLQQNY
                     FAKLDDLTETEKAKLPKRIFIFGISSFSPLQLAVFKKLSEHCDIHLFFFNPSEKYWGD
                     SVEEKVWQKLALSHKISQDDLDNLLAEQRNKLLNLWGKQGRDFLAQLIEFEPNTIDVF
                     VEPEENSNLNKLKKQIFSLENDATITDLTADNSLQIHSCHSPMREVEVLHNQLLNLFE
                     QDSTLLPKDIIVMSPDINKYAPYIDAVFSRYNTGNRDPRYIPFSLSDQAFTQVDPIIH
                     SFLQLLAMKESPFNAEELFDLLEVNAIQTRFGFSTEDIHTLRVWVRNAGVRSGVEIEQ
                     PHWQNYNSWENGLNRLLLGSSLKEEQGIWENTLAFGDSYGLNAELVGRLASFIEALLA
                     WVNFLQTPQPLAAWQQAIKNLTADLYQENAENAASILLLNNVNDQIIEQISATHFSDD
                     LQIEVLALLFEQQLSQHSQQLNFLVGRVNFATLLPMRAIPFKVICLLGMNEADFPRQQ
                     QINSFDLMQYAPKKGDRAKRDDDRYLFLEALLSAQQMFYISYIGQSQKDGKEMLPSIL
                     VSQLLDAMNEALTPECKQQLSDEVEVLVRKQPLSVFSKKNFTHPRDHAYNAEWIIKPT
                     VAVQDFLSEPLTNESVASLELDELISFVQNPIRFFFLKVLGISFNTYDDTIDESEIFE
                     MSGLDNYRILNELLTINLAQQQRFFAEEQLKGNLPASYFAQIATQDLIGKITEMQAAL
                     SHYLASESRILSVNREVELERQTLTLNGNIANLYQNEVVLWRVGHLRDKDKIQTWIYH
                     LLLCAYFPEKTVKFYYREGEQVGVLSFETVSQIEALSQLSIYLNAYLDGLSQAQLVIY
                     DRIEEYLKGDGTNSEQVLIDLAEKDERDGSYLHRLLAQTKELDSQAIHQRTQSWFSLM
                     NQKIAKGD"
     gene            4523..7468
                     /gene="ptrA"
                     /locus_tag="PWX02_000676"
     CDS             4523..7468
                     /gene="ptrA"
                     /locus_tag="PWX02_000676"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HDL1728714.1"
                     /translation="MKLSFLTQFVAAAFALNVMTYPAFAEVAKNSEKTTASQPQSQLG
                     FELIKQSINKSPNDKAIYQGIKLDNGMEVLLISDSQANKSLMSAIIPIGSMDDPISQQ
                     GLAHYLEHMILMGSKNYPETNSLDGFLTKNGGMNNASTASHRTAYYLQVNNEAFSEAV
                     TRLADTLAFPLLLETNAKKEVNAVNAEMVRAKSHDGHLLHSVNLATANPAHPATKFAV
                     GNKETLSDKPDSKLQTELEQFYKQHYSANLFKVVLYSNQSIEQMAKLAANTLGKMENK
                     HFSIPQTAEPRYRDEDKSVFIQYKPVKSNKMLVIGFDFPNDEMQFKHKTGKYIAYMLN
                     NNTDGTLSDYLIKQGLSDGGIEAVSSANIGRDRGSFDIYIELTDKGLSEQDQIISLVF
                     QQIEKIKQEGIQESYFSEVKESLKQDFQHLQVEKNMDFVEFIADQMLHFPLEHVIDQP
                     YLTETMDNEAIKAKLAAMNIDNARIMLVSDTAKTDKKTPYFEAGYSVAKITQEQKQKW
                     LDFSKNPQLNLPELNPYFTTDFTLNKVETGLTKPKLINDQAGNLIYTMPSVYFTNEPK
                     AKIGVGFNIRPRKDDLKSLTAASILGYMNNLAQTKLDFQSSVAGMNLSLAPAANGLAL
                     SAEGYTQNLAKLMLDSVKQFSRFEFNEDVLAQAKQRLHQELDRNEKENSLNQANAALS
                     RFASYPYFDVPKQREMIDSITLADIEQLRQRILQQSSGFKLLSVGNLSAEQVSALAED
                     LQAVIKNQKSANATGQYVDVSTSQRKINVIKSVPNEDNALLMAFMPNGYNELESLTRA
                     KLLRSIISRWYFDDLRTDKQLGYVVYATDNIIGKTAGIQFMVQSPNTTPAGILEHNER
                     FFTQSFERLKNLSDEEFGKYRDSLVEKLQYKPESLAEEFSEFGSDFSRNNPKFDRLQQ
                     VIELTKALTKADIVAFYQKAVIEKQGFVFVSQAVGTKTKAEDVAKFDGFEQIESIEKL
                     QREFEIKDY"
     gene            complement(7517..9163)
                     /locus_tag="PWX02_000677"
     CDS             complement(7517..9163)
                     /locus_tag="PWX02_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1728715.1"
                     /translation="MKFPTLTKISASVLAVLALAACDDKSNENKPTAAAAKPQTEKTF
                     VNCVSRSPTGFSPILMMDGLSYNASSQQIYNRLVEFVPGTTDIEPALAESWEISEDGL
                     TYTFKLRKGVKFHSNKDFTPSRELNADDVVFSFNRQLDKNHPYHTVSNGTYPYFNAMK
                     FPTLLKSVEKVDDSTVRITLTKRDASFLSSLGMDFTSIYSAEYADKMMKAGIPEAVDT
                     TPIGTGPFVFSGYVLDQASRYVANKEYWKGKPDIDRLIFEIVPDATTRYAKLQSGQCD
                     LMDFPNATDIEKMKNDPKINLLSQPGLNIAYVAFNTEKAPFDNVKVRQALNLAVDKKA
                     IIDVVYQGNGFAAKNPLPPTIWGYNDSLADSEYNVEKAKQLLAEAGFPNGFETELWVQ
                     PVVRASNPNPRRMSEIIQADWEKIGVKAKLVTYEWGDYIKRTKAGELTAGTYGWSGDN
                     GDPDNFLSPLFGTPNIGNSNYARFTNPELDALLEKALVSSDKAERTKLYEQAQVILRE
                     QAPWINVAHSINFAPTSKRVEGYKQSPFGYTYLYPVKIVD"
     gene            9458..11596
                     /locus_tag="PWX02_000678"
                     /pseudo
     CDS             9458..11596
                     /locus_tag="PWX02_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694042.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
     gene            11667..13355
                     /locus_tag="PWX02_000679"
     CDS             11667..13355
                     /locus_tag="PWX02_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HDL1728716.1"
                     /translation="MKFYPSLIASAVSFAVVLSGQAAANVPDAGSLQRELDQRQAQQK
                     VKPSGEWFNQAAKAPYTATDGQELTFPLTQVQVFDWTNQEISTPELAPILANYVGKAV
                     SLTDLSNLANEITEFYRSHNYLVAKAILPPQEIEQGKVKILLFKGNVGEVRLQNQSAL
                     SDKFVARLANTTVNGSEFILKDELEKFALTMNDVPGVNTGLELSAGKQAGEANLLIKL
                     QNAERFSGYVSADNQGNKNTGRYRLATGIKANNLIGWGDELKVDLMSSNNANLKNARL
                     DYSSLIDGYATRLGLTANYLDYELGGNFKALQSQGHTHSIGAYVLHPTIRLPDFRLNT
                     KVSFNHQGLTDKQQAVNVAQKRQVNSLTVGINGSWNSLKDGTSYFSLSALFGNLANHT
                     NEQAHYVATDFQPKSRFTVYQYSLSHEQALPKSFAFNLGVNGQFADKTLDSSQKMLLG
                     GLYGVRGHQAGVASVDEGHLVQTELKHYLPLFSKSLLTSSVFYDYGWGKYYKHSGFLA
                     RGVNNSVKLQSIGAGLSLSEAGSYAINASVAKPLGKKIENADKHQFWFSAIKTF"
     gene            13367..16189
                     /locus_tag="PWX02_000680"
     CDS             13367..16189
                     /locus_tag="PWX02_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HDL1728717.1"
                     /translation="MFKRNAISLCILTACSGLAYANTADLPQGERVRVGTATIERTTD
                     KMTVNQSSNKVQIDWNSFDIGKNKEVEFKQPTEMAVAYNRVTGGNASQIQGKLTANGK
                     VYLANPNGVLITKDAEVNVGGLLVTTKDLEKISENGTTKDKFSRKLAKEGKILNQGKI
                     TAKGERAFVVLVGDDVANEGEINAENYYKKETKRVRECYNDPWSWGQQCYDIDKEIVK
                     STSGEVHLSSGENVTFTLSDRYIDVALDDNTVNSIIKNEGAIIANGDITFTAKGRNQA
                     LDSLIINNGVLQANKVQNKNGTIILSAGETQLHEKSQIKGEKVSFAEASYYDGPKENI
                     KVVSKTGSKVTAPVIDFKGKTVNIQGSFGREDRTEHYNDELKTLRTVVNIEVPNTEDI
                     RIADTDNGGSGSFIQAGALGSLLANNGRVNVQGKGFDVSGKLNVNAFKQTDSVLKFIN
                     QADINIHNADIHSNGRLFMLTNLPNDTDFQSNIRITDSKINLGNGAMGFGRSAKRWEM
                     HDVVRRADTELRKKFNLDMKNVELNQVDDFVVAGGFQQVNLDNVKARGKTNFYLDSGN
                     SRVFVGEQIYEYGVTDIEKRTQLANLDQRRKRWNSDNYDEIDLNWEYNQRFDGFAAKH
                     NHRAPIAGTEINIANSDIGLDNGFVHLMAEKINLDDSKINITFNQDNSLDESTQINRL
                     GLNGKVSMKNSHINVVGDEKDGISPARHYATMFLVGELLGEKSSIFLKSHQGAVLRTD
                     SNVKIAGKNSKDDLKITAITTGKKMGNEIIGPDGLPTSEDNDANASNTAFAIGYEPNA
                     TAIVENASITALAPNGGLAYTGTSKTQIDAKDNATFTFFEKPRAGYDKLLGDIKGNPD
                     KLTPRDFARLYDNIKGIKASSLSEQQLGLADTAKKTSEHTLANSLDVENLVSVTVCDE
                     QNKCEERMLGNKNTNAKVIVGDIKFE"
     gene            16312..16443
                     /locus_tag="PWX02_000681"
                     /pseudo
     CDS             16312..16443
                     /locus_tag="PWX02_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587011.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(16498..17259)
                     /locus_tag="PWX02_000682"
     CDS             complement(16498..17259)
                     /locus_tag="PWX02_000682"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDL1728718.1"
                     /translation="MKLNQAQIQNALICGQAVVFDEIDSTNEYLLTHHQRLENGTVCL
                     AEKQTAGRGRRGRTWYSPESENLYFSILWHYPMEQAADLPPLSLVVALIIAESLTAQN
                     VKDIQIKWPNDIYYQGKKMGGILLETQANRNGLDVVIGIGLNLGMAKVDENIVTQAWA
                     DLSQYHFNRNELVCRLAYELQKNLKIYPLVGFAHYAERWQSFDLFRHKAVKLITEAEE
                     IHGISQGINEQGELILKQGDNLRYFGIGEISLRAD"
     gene            complement(17271..18302)
                     /gene="murB"
                     /locus_tag="PWX02_000683"
     CDS             complement(17271..18302)
                     /gene="murB"
                     /locus_tag="PWX02_000683"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDL1728719.1"
                     /translation="MMKHSLTPFHTFHLPAKATQIIEFTTVEQLLSEWQKAFNAQLPI
                     LILGQGSNVLFLEDFDGVVLVNKLKGITHHEDEHFHYLHVQGGENWHELVKWTLAHNI
                     AGIENLALIPGVAGSAPIQNIGAYGVEFEQLCDFVEVLNLRSGERFKLSKSECEFGYR
                     ESVFKHQYRDEFAIISVGLKLAKAWKPVLSYGSLAQLNPETVTPKQIFDEVCAVRSSK
                     LPNPDEFGNAGSFFKNPVISNEQFAPIQTKFPTIPHYPQANGSVKLAAGWLIDQTGLK
                     GLQIGGAAVHTQQALVLINKENATGQDVLALAKTVRQRVREKFGVEIQPEVRFIGKNG
                     EVDSEKITR"
     gene            complement(18406..19080)
                     /gene="crp"
                     /locus_tag="PWX02_000684"
     CDS             complement(18406..19080)
                     /gene="crp"
                     /locus_tag="PWX02_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010787261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HDL1728720.1"
                     /translation="MQDMTLSTAPLENVTPPPSIHDPAVEWFLTHCHIHRYPAKYTLI
                     HAGEDAESLFYIVNGSVSVYIKDDETKEMLLTNLGTGEFVGELSLFEEKVQQRTAYVR
                     TKDPCEIAEISYKKFKQLVHLNPDILMYLSAQMARRLRQTSRQVGNLAFLDVAGRIAQ
                     TLLNLAKMPDAMTHPEGMQIKITRQEIGQMVGCSRETVGRIMKMLEDEGLISAHGKTI
                     VVYGAR"
     gene            complement(19142..19360)
                     /locus_tag="PWX02_000685"
     CDS             complement(19142..19360)
                     /locus_tag="PWX02_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HDL1728721.1"
                     /translation="MIIPWQELEESTLHNILDSFILREGTDYGERELSLAEKRERLLT
                     QLKADKVVIVWSELHESLDIKDKKSFLG"
     gene            complement(19363..19968)
                     /gene="slmA"
                     /locus_tag="PWX02_000686"
     CDS             complement(19363..19968)
                     /gene="slmA"
                     /locus_tag="PWX02_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HDL1728722.1"
                     /translation="MTEPKIKMPKKSVVERRQQVLEVLISLLNSEEGMQRITTERLAT
                     AVGVSEGALYRYFPSKTKMFEALIEKIEQTLTSYIHANKRQDSSANSVRAILYVILEF
                     ARKNPGVTRILTGHALMFEEELLKARVAKFFDNLELQFANILQLSKLRERKSFEDERA
                     LAGYLVNFCEGQFLRLVRSNFSYNQHQHFEKQWAFIKPLFD"
     gene            complement(19980..20435)
                     /gene="dut"
                     /locus_tag="PWX02_000687"
     CDS             complement(19980..20435)
                     /gene="dut"
                     /locus_tag="PWX02_000687"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014550997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDL1728723.1"
                     /translation="MKQIDLKILDSRIGNEFPLPAYATEGSAGLDLRAMLNEPLTVKA
                     GETVLIPTGISIYIADPNLAAVILPRSGLGHKNGIVLGNLIGLIDSDYQGELKVSLWN
                     RSQADFTVNVGDRIAQLVFVPVVQASFNIVQEFEQTDRGEGGFGHSGKQ"
BASE COUNT         6151 a         4290 c         4483 g         5586 t
ORIGIN      
        1 aatagaatca cctaaacaaa aaggattgat ttatacggca gtacgttatg tttttatctt
       61 aaataatacg aggtaaaagt gaataaatat aaaaatgcca gtgcattatt ggtttcatta
      121 atacttattt ctacaatttt gtcggtttta tttttaagta aagaaaaatg gcttggacaa
      181 caaaacatta cccattttta tagagagaaa tacctttccg atcaatataa ttttttagcg
      241 ttatatccac aagataaaac gcaaatatgc caaaaatttc aaaaagagca gatcagccaa
      301 gaggatttat ccgataaaca attcaggttt ttacagtatc aatttgcctg ccaattccat
      361 agtttattta aagataaaaa accgactaaa gagaaatata ttcactttac ccgtttggcg
      421 gattatttag atttatcaaa tgtgccaaac caagagattt atactattca ttctttagct
      481 gaattaccta gtagcagtga acgtgagcct aaaattgtgc ttgctaaaaa cgcaattaat
      541 gaaaacttac cgcaagattt ttacggcatt gtgataaccg actatctgtt tgatattacc
      601 ggcaaaaaaa tgtacggcac gctttattcc tcttatgata atgcccgaga ggagcgaaat
      661 ttaacgttta aaaaagaggt actggcagag cttgaggcga ggtattccgc ttggcgatat
      721 ttgccatatt ccgaacattt gatgggagcg gttgatgatt aaatataaag cagaaacggc
      781 tttgtcgctt ttggttgcca tcacgctatt tgcgattgtg gtgttgagtt tcagccagtg
      841 gcaatcggca caaaaccaca ggctaaatca acatttccaa cagcaacaag cggttatgat
      901 tttagaaaat cagcttgccc tcaaattagc cgggttagat tgtgaatcaa gtattactca
      961 aaatcagatt cgctatcaga ttcaatgctc tggcaatcgc ttaaccattc gctttccgtt
     1021 aggcaaaatt gagctgaata atgattgaat cgtccctcgc tttggaggac aataattcaa
     1081 ttagcgaggc gttattatgt ttacacttta ccaatccaac caaatttcat cactggcgga
     1141 gatgctggtg aaaatccagc aggtaaaccc acttgaagat ccgtttgagc cggaaaccat
     1201 tttgatccaa agtcagggaa tggcacagtg gctacaaatg cagattgctg aattaaacgg
     1261 tgtgatgggg aattgcgatt ttctctaccc tactacattt ttatggcaac agtatcggct
     1321 gttgttcccg gagctgccga aagagaatat ttttgagcgt agttcgttgg tttggcggat
     1381 tatgcggtta ttgccgagtt gcttgacact atcggagttt gctccgctaa agcgttattt
     1441 aggtgatcaa cctaataaag aacagtatca gcttaagctc tatcaacttt ctgccaaaat
     1501 tgccgaattg ttcgaccaat atttggtgta tcgcccccat tggctggtta gctgggaaga
     1561 aggcaactta caagcgatcg aaaatgagat aaaaattgca actgccgcta agccgattga
     1621 ttttgactta attcgagccg atatgaaatg gcaaagccat ttatggcaac agattgtatc
     1681 tgatctgcaa tcggaatctg atgagcaggt ttttactacc tcccaccgtg cctatttgca
     1741 acaaaattac ttcgctaagt tagatgacct tactgaaacg gaaaaagcca aattgccaaa
     1801 acgcattttc atttttggga tttcgtcctt ctcgccatta cagttagcgg tgtttaagaa
     1861 actcagcgag cattgcgata ttcacctgtt cttttttaac ccaagcgaaa aatattgggg
     1921 cgacagtgtg gaagaaaaag tgtggcaaaa attggcgtta agccacaaga tttcgcaaga
     1981 cgatctggat aacttattgg cggagcaaag aaataaacta ctgaatcttt ggggcaaaca
     2041 agggcgggat ttcttagctc aattaatcga atttgagccg aataccattg atgtctttgt
     2101 cgaaccagag gaaaacagca acctcaataa actcaaaaaa cagattttca gcttagaaaa
     2161 tgacgccact attaccgatt taaccgccga taattcactc caaattcatt cgtgccacag
     2221 cccaatgcgg gaagtggaag tgttgcataa ccagctcctg aatttgtttg agcaagatag
     2281 cactttgctg ccgaaagata ttattgtgat gtcgccggat attaataaat acgctcctta
     2341 cattgatgcg gtgttttctc gctacaatac tggtaatcgg gatcctcgct atattccttt
     2401 ttcattatcc gaccaagcat ttacgcaggt tgatccgatt atccacagtt tcctgcaact
     2461 gctggcaatg aaagaaagcc cgtttaatgc cgaagaactg tttgatttac tggaagtgaa
     2521 tgcgatccaa acccgttttg gctttagtac ggaagatatt cataccctgc gggtttgggt
     2581 gagaaatgcc ggcgtacgtt cgggtgtaga aatcgagcaa cctcactggc aaaactacaa
     2641 ttcgtgggaa aacgggctaa accgtttatt gcttggctca agcctgaaag aagagcaggg
     2701 gatttgggaa aacacccttg cctttgggga tagctatggc ttaaatgccg agctggtggg
     2761 gcgacttgcc tcttttattg aggctttatt agcgtgggta aactttctgc aaacgccaca
     2821 gccgttggca gcgtggcagc aggcaataaa aaatttgact gctgatctgt atcaagaaaa
     2881 tgccgaaaat gctgcatcaa ttttattgtt gaataatgtg aatgatcaga ttatcgagca
     2941 aatttccgca acccattttt cagacgatct gcaaattgag gttttagcct tattgtttga
     3001 gcagcagctc agccaacatt cacagcagct taatttcttg gtcggacggg tgaattttgc
     3061 caccttgctt ccgatgcggg cgattccgtt taaggtaatt tgcttgctgg gtatgaatga
     3121 ggcagatttc cctcgccaac aacaaatcaa tagctttgat ttaatgcagt acgccccgaa
     3181 aaagggagac cgtgccaaac gtgatgatga ccgctacttg ttcttagaag ccttactttc
     3241 cgctcagcaa atgttttaca tcagctatat cgggcagtcg caaaaagacg gcaaagagat
     3301 gctaccgtcg attttggttt cccaactgtt agatgcaatg aatgaagctt taacgcctga
     3361 atgcaagcaa caattaagcg atgaagtgga agttttggtc agaaaacaac ctttaagtgt
     3421 gtttagcaaa aagaatttta cccacccacg agatcacgct tataacgcag agtggattat
     3481 caagccgact gttgccgtac aagattttct tagtgagcct ttaaccaatg aaagcgtggc
     3541 ttcgctagaa cttgatgaat taatctcttt tgtacaaaac ccgattaggt ttttctttct
     3601 aaaagtgttg gggatttcgt ttaacactta tgatgacacc attgatgaaa gcgaaatttt
     3661 tgagatgtcg gggctggata actaccggat tttaaacgaa ttattaacga taaatttagc
     3721 acaacagcaa cgtttttttg ccgaagagca actcaaaggc aatttacctg cttcttattt
     3781 tgctcaaatt gccacacaag atttgatcgg caaaattacc gaaatgcaag cggcgttaag
     3841 ccactattta gccagcgaaa gccgtatttt gagtgttaat cgggaggttg agctggaacg
     3901 ccaaaccctc accctaaacg gcaatattgc caacctatat cagaatgaag tggtgctatg
     3961 gcgagtaggg catttacgag ataaagacaa gattcagact tggatctacc atttattatt
     4021 gtgtgcctat ttccctgaaa aaacggtgaa attctattac cgagagggtg agcaagttgg
     4081 tgtgttgagc tttgaaaccg tctcgcagat agaggcatta tcgcaattaa gcatttattt
     4141 gaatgcttat ttggacggat tatcgcaagc ccagttagtg atctacgacc gaattgagga
     4201 atatctcaaa ggtgatggca caaacagcga acaagttctt atcgatttag cagagaaaga
     4261 tgagcgagac ggcagctatc tccatcggct tctcgctcaa actaaagagc tggattctca
     4321 agcgattcac caacgtactc aaagctggtt tagcttaatg aatcagaaaa tagcgaaagg
     4381 cgattaacta caagcggtcg tttttggctg aaaatttgca aaatgagaag gcgaataata
     4441 attcgcctac ctataaaaat agaatttttt ccgtatttta ctgtcaattt attactacaa
     4501 tgctaatttc aaaaggagat gtatgaaatt atcttttctt acccaattcg ttgcagcggc
     4561 ttttgccctc aatgtgatga cttaccctgc atttgcggaa gttgcaaaaa attctgaaaa
     4621 aacgaccgct agtcaacccc aatcccaact cggttttgag ctgattaaac agagcattaa
     4681 caaaagcccg aatgataaag cgatttatca aggtattaaa cttgataatg gaatggaagt
     4741 gctactgatt tccgacagtc aagcgaataa atcgttgatg tctgccatta ttccgattgg
     4801 ctcaatggat gacccgattt ctcagcaagg tttagcccac tatcttgagc atatgatctt
     4861 gatgggctcg aaaaactatc cggaaaccaa cagcttagac ggttttttaa ccaaaaatgg
     4921 cggtatgaat aatgcctcga ctgcttctca ccgcacggcg tattatctac aagtcaataa
     4981 cgaagccttt agcgaagcgg taacacgact ggcggatacg ttagctttcc cgttattgct
     5041 ggaaaccaac gccaagaaag aggttaatgc ggtcaatgcg gagatggtgc gagctaaatc
     5101 gcacgatggg catttattgc acagtgttaa tttagccacc gcaaacccgg ctcatcctgc
     5161 taccaaattt gcggtaggca ataaagagac gttatctgac aagccggata gcaaattaca
     5221 aaccgagttg gagcaatttt ataaacagca ttattctgcg aatctgttta aagtggtgct
     5281 ttattccaat caatcgattg agcagatggc aaaattggca gccaacactt taggcaaaat
     5341 ggaaaacaaa catttcagca taccgcaaac cgctgagcca cgttatcgtg atgaggacaa
     5401 atcggtattt attcaatata agccggtgaa atccaacaaa atgttagtca ttggctttga
     5461 ttttccgaat gacgaaatgc agtttaagca caaaaccggt aaatatattg cttatatgtt
     5521 aaacaataat accgacggca cgctttcgga ttacttgatt aaacaggggt tgtctgacgg
     5581 cggtattgag gcggttagca gtgccaatat cgggcgggat cgaggctcgt ttgacatcta
     5641 tattgaactg acagacaaag gtttgagcga gcaagatcaa attatttcat tggttttcca
     5701 acaaattgag aagatcaaac aagaaggcat tcaagagagc tattttagcg aagtgaaaga
     5761 gagcctgaag caggatttcc aacatttgca agtggaaaaa aatatggatt tcgtggaatt
     5821 tattgccgat caaatgctcc atttcccgtt agaacacgtt atcgatcagc cttatctgac
     5881 cgaaacaatg gataacgagg caattaaagc aaaattggcg gcaatgaata ttgataatgc
     5941 ccgcattatg ttagtcagcg atactgccaa aaccgacaag aaaacccctt attttgaagc
     6001 aggttattcg gtggcaaaaa tcacgcaaga acaaaagcaa aaatggctgg atttcagtaa
     6061 aaatccacag ctcaatttgc ctgaattgaa cccgtatttc actaccgatt ttaccttaaa
     6121 caaggtcgaa accggcttaa ctaaaccgaa attgattaac gaccaagcgg ggaatttaat
     6181 ctatacaatg ccgagcgtgt attttaccaa tgaaccgaag gctaaaatcg gcgttggctt
     6241 taatattcgc ccgagaaaag atgacttaaa gagcctgacc gcagcgtcta ttttgggtta
     6301 tatgaacaat ttagcccaaa ccaagttaga ttttcagtct tcggtggcgg gtatgaattt
     6361 aagtttagct ccggcggcaa acggtttagc cttaagtgca gagggttata cccaaaatct
     6421 ggctaaatta atgctagaca gcgtaaaaca gttcagccgc tttgagttta acgaagacgt
     6481 gttggcacag gctaaacaac gtttgcacca agagctggat cgtaacgaaa aagaaaacag
     6541 ccttaatcaa gctaatgcag cattaagccg ttttgcctct tacccttatt ttgatgtgcc
     6601 gaaacagcgt gagatgattg atagcattac ccttgcggat attgagcaac tacgccagcg
     6661 aattttgcag cagagcagcg gctttaagtt gttatcggtg gggaatttgt cggctgagca
     6721 ggtttctgct ctggcagagg atttacaagc ggtcataaaa aaccaaaaat ctgcaaatgc
     6781 taccggacag tatgtggatg tgagtaccag tcagcgtaag attaatgtga ttaaaagcgt
     6841 gccgaatgaa gataatgcgt tgctaatggc atttatgccg aacggctata acgagctgga
     6901 aagccttact cgagccaaat tgctcagaag cattatttcc cgttggtatt ttgatgattt
     6961 gcgtactgat aagcaactag gctatgtggt ttatgcgaca gataatatca tcgggaaaac
     7021 cgccggtatc cagtttatgg tacaaagccc gaataccaca ccggcaggga ttttagagca
     7081 taacgaacgt ttctttacac aaagtttcga acgtttaaaa aatctatctg atgaggaatt
     7141 tggaaaatat cgtgatagcc tagtggaaaa actgcaatat aaaccggagt ctttagcgga
     7201 agagttttct gagttcggtt cagactttag cagaaataac ccgaaatttg accgcttgca
     7261 acaggtgatc gaattaacca aagcactcac caaagcagat attgtggcat tttaccaaaa
     7321 agctgtgatt gaaaagcaag gcttcgtgtt tgtcagccaa gctgtcggca ccaaaaccaa
     7381 agctgaagac gttgcgaaat ttgacggttt tgaacagatc gaaagcattg aaaagctaca
     7441 acgagagttt gagattaagg attattagag agttattaga gagagttagg ggcgaaaaat
     7501 tttcgcccct cattttttaa tccacaattt tcaccggata taagtaagta tatccgaacg
     7561 gactttgttt gtagccttca acacgtttgc tggttggggc aaagttaatt gagtgagcca
     7621 cgttaatcca cggagcttgc tctcttaaga taacctgtgc ctgctcgtat aacttagtac
     7681 gttctgcttt atcagatgag actaatgctt tttctaataa cgcatctaac tcagggttag
     7741 taaagcgagc atagttactg ttgccgatat ttggcgtgcc gaatagtggc gataagaagt
     7801 tatccggatc gccgttatcg cccgaccaac cgtaagttcc ggcggttaat tcaccggctt
     7861 tggtacgctt gatgtaatca ccccattcat aggtgactaa ttttgctttt acgccgattt
     7921 tttcccagtc ggcttgaata atttcagaca tacggcgtgg gttcgggtta gaagcacgca
     7981 ccacaggctg cacccataat tcggtttcaa aaccgttcgg gaagcctgct tcagctaata
     8041 gttgcttcgc tttttccacg ttatattctg aatccgctaa gctgtcgtta tagccccaaa
     8101 tggttggcgg aagtgggttt ttcgccgcaa aaccgttacc ttggtaaact acatcaataa
     8161 tcgctttttt atccactgct aagtttaacg cttggcgaac tttcacgtta tcaaacggag
     8221 ctttttcggt attgaatgcc acataagcaa tgttcaaacc cggttgagaa agtaaattga
     8281 ttttcggatc atttttcatt ttctcaatgt cggttgcatt tgggaagtcc attaaatcac
     8341 attgaccgga ttgtaatttc gcataacggg ttgttgcatc cggcacgatc tcaaaaatca
     8401 aacggtcaat gtcaggctta cctttccaat actctttgtt tgctacataa cgactggctt
     8461 ggtctagcac ataaccgcta aacacaaacg gacctgtgcc gattggcgtg gtatctaccg
     8521 cttcaggtat gccggctttc atcattttat ccgcatattc cgccgaataa atggaggtga
     8581 aatccatacc aaggctggat aagaatgacg catcacgttt ggttaaggta atacgaacgg
     8641 tgctgtcgtc cactttttcc accgatttca ataatgtcgg gaatttcatt gcgttgaaat
     8701 acggataagt gccgttagag acggtgtgat acggatgatt tttatctaac tgacggttaa
     8761 atgaaaatac tacatcgtcc gcattcaatt cacggcttgg agtgaagtct ttgtttgaat
     8821 ggaatttcac gcctttgcgt aatttaaagg tgtaagttaa gccgtcttcg ctgatttccc
     8881 agctttctgc caatgccggt tcaatatcgg ttgtacccgg cacaaattcc accaagcggt
     8941 tgtaaatttg ttgcgagctg gcgttataag ataaaccgtc catcattaaa atcgggctaa
     9001 aaccggttgg cgaacggcta acgcagttta caaaggtttt ctcggtttgc ggttttgctg
     9061 ctgccgcagt aggtttattt tcgttgcttt tatcatcgca agccgctaga gctaataccg
     9121 ctaaaacgct tgcactaatt ttggttaacg ttggaaattt catattattc tccgcttaat
     9181 cttaggatta acttacgaag attacctttt ccacaatgag gagtaaagaa caaaaagcat
     9241 tttgttataa cgaaatgata taaaaaataa agtacggatg cttatttttc aaaatttcag
     9301 ataagaaagg aaagatagta gctaattctt aagataaccc tattttaaac gtgtatataa
     9361 aaccttcata tacaaataat tctcattagc taattgatag caattctcaa tagtgttata
     9421 ttattgctct accttatacc taaaaagagg agtaaaaatg caactttcta aactatccct
     9481 tgcgattact gttagtttat tctctgcaaa tatttttgca aatcaatcca tagaattaga
     9541 taccgtcaac gtcattgcca cccgtgatcc aagtaaattt gctgatacgc cacaaaaaca
     9601 aactaaagat gcacttttgg taaaacaagc gacgagtgtg gcggtggcat taaaagcgtt
     9661 gccgaatgtg gatgtgcaag gaggggcccg tgcgattgcg tagaagccgt ctattcgtgg
     9721 gttgagcgat aatcgtgttg tgcaggtgat tgacggagtg cgtcagaatt ttgatttggc
     9781 tcaccgtggt tcttattttg tgccgatgtc attgattcaa gaaattgaag taatcaaagg
     9841 cccaagcagt tcgttgtggg ggagcggtgc cttaggtggt gtggtggcaa tgcgcacacc
     9901 gaatgcgttg gatttattga aaaataatga caaattcggg gcaaaaatcc gtcaaggtta
     9961 tcaaacggcg aataatcttt ccgaaaccga agcctcggtg tttgcagcaa atgatcggtt
    10021 tgatgccttg ctgagtggtt tctacaataa tgctgacaat ttgcgtagtg gtgaaggcag
    10081 ggagctgaat aacaccggct ataagcaagc cggtggcttg gtaaaattcg gttggcaact
    10141 gaatgaggca aaccgtgtgg agctttcaca ccgtgagagc caatttaagc aaacagcacc
    10201 aagtaacaat gatgtcgaaa atcaatttac cactgcagac atcaacgcca aaattgcggc
    10261 gtggcacgca gccaatccgc cttcaacacc gaatttcttt gcgaaattag cggcatttta
    10321 tggcggtttg ggcgcacaat ttggtagcgt gagttatttg agcgatcaaa aaattaacga
    10381 taaaagcacc gcacttaact actatttcaa cccgagcaat ccttatgtca atgcgcaagt
    10441 gacagtctat caaaacagca ccacggaaaa agagcaacgt aaagtgagtg gtgtggaaga
    10501 tcgaaccaaa ttaaccacgc gcggcatcaa tctgcgtaac cattccgaat tgccttggtt
    10561 ctcgttggtc tatggtgtgg attatatgca ggataaagcc gacaccgagc gtggcacaaa
    10621 caatagtgat gcccgctatc gttctaatcc gtatcaagca aaatccaata cgacaggtgc
    10681 atatttaatc gcccatattc cgctttgggg cgaaaaactg ctgttctcgc cgagtgtacg
    10741 ttatgaccgt ttcaacacct caagcgaggc ggtgaaatac caagacagcc attggtcgcc
    10801 ggcggcaaaa ttgacatgga aagcgacaac ttggttagat tttaccgcaa aatataatga
    10861 agccttccgt gcgccttcaa tgcaagagcg ttttacaggc ggttcgcact ttggtactca
    10921 aggacagggc ggtgcaccga ttatcaatat ttttgtacaa aacccgaatt tacgtcctga
    10981 aactgcgaaa aataaagaga tcactgccca tgtgcatttt gatgatctga tcacacaagg
    11041 ggataaattc agcgttgaag ccacttattt ccgtaacgat gtgagggatt tcatcaatct
    11101 tcaactttac aaagccaatc cggcaagccc gatggaagtg attccaaccc gttcgcaata
    11161 tcgcaatgta accaacgcac gattaagcgg tgtggagtta caaacccaat atcaaaccga
    11221 gcgtgttacg ttcttggcaa actacggctc aaccaaaggt aaagataaag acagcggaga
    11281 agccttgtcg aacattgctg caagcaaaat cggctttggg gtcaattatg ccgtggtgca
    11341 agacaaattc acggtggggg caaacgtaac ccgttatcaa gcacaacatc gtgtgccgaa
    11401 aaaccacggt gtgacttacc aaggctatac gctgaccgat ctacacgcca cttacgcccc
    11461 attaaaaggc gaatggaaaa acctgcgttt agacttggcg gttgaaaacc tattcgataa
    11521 aaaataccaa ccggcattca gcttaatgga aggcagcggt cgtaatgtga aactcagtgc
    11581 ggcttacagc ttctaatgtc tttaacggaa gacggcacaa gcggtcgttt tccggttatt
    11641 ttttgcaata ttaaaaggga gttactatga aattttatcc gtcgctgatc gcaagtgcgg
    11701 tcagttttgc cgttgttttg agtggacaag cggcagcgaa tgtgccggat gccggctcgt
    11761 tacagcgtga attagaccaa cgtcaagctc aacaaaaagt caaaccgagt ggcgaatggt
    11821 ttaatcaagc ggcaaaagca ccttataccg caacggacgg tcaagaactg acatttccat
    11881 taactcaggt acaagttttt gattggacca accaagaaat ctctaccccg gaattagccc
    11941 cgattttggc aaactatgtg ggtaaagcgg tttcgctgac cgatttaagc aacttggcaa
    12001 atgagatcac cgagttttat cgcagccaca attatctggt ggcaaaagcc attttaccgc
    12061 cacaggaaat tgaacaaggt aaggtgaaaa tcctgttgtt taaaggcaat gtcggtgaag
    12121 tgcggttaca aaatcagagt gctttaagcg ataaatttgt tgcacgcctt gccaacacca
    12181 cagtgaatgg ttcggaattt atcctaaaag acgagctgga aaaatttgcc ctcacgatga
    12241 atgatgttcc cggggtgaat accggcttgg agctttctgc cggtaagcaa gcaggcgagg
    12301 cgaatttgct gattaagctc caaaatgccg agcgtttctc aggctatgta tcggcagata
    12361 accaaggcaa taaaaatacc ggacgttacc gtttagccac agggataaaa gccaataatc
    12421 tgataggttg gggcgatgag ttgaaagtgg atttgatgag ttccaacaac gctaatttaa
    12481 aaaatgcgcg tttggattat tcgagcttga ttgacggcta cgctacccgt ttgggcttaa
    12541 cggcgaatta tctcgactac gaattaggcg gtaactttaa agccctacaa tcccaagggc
    12601 atactcacag catcggggct tatgtgctgc acccgacaat ccgcttgccg gattttcggt
    12661 taaacaccaa agtcagcttt aatcatcaag gtttaaccga caaacaacaa gcggtcaatg
    12721 tggcacaaaa acgccaagta aacagcttaa cggtgggcat taacgggtcg tggaattcgc
    12781 ttaaagacgg cacaagctat ttctctctgt ctgccttgtt cggcaattta gctaaccaca
    12841 ccaacgagca agcgcattat gtggcaacag attttcagcc caaatcccgt tttacggtct
    12901 atcaatacag cctgtctcac gaacaagcgc tgccaaaatc ctttgccttc aaccttggcg
    12961 tgaacgggca gtttgcggat aaaaccctcg acagctcaca aaaaatgtta ctgggcggtt
    13021 tatacggcgt gcgtggtcat caagccggtg tcgcttcggt ggatgagggg catttagtgc
    13081 aaaccgaact caagcattat ttaccgctct tttccaaaag cctgctgacc agctcggtct
    13141 tctatgatta tggttgggga aaatactata aacacagcgg atttttggcg cgtggtgtaa
    13201 acaatagcgt gaaattacag agtatcggag cagggctttc gctctctgaa gcaggcagtt
    13261 atgccattaa cgccagtgtt gccaaaccgc tgggtaaaaa aatcgagaat gcagataaac
    13321 accaattctg gttctccgcc atcaaaacct tttaaggaga caatttatgt ttaaacgtaa
    13381 tgcaatttct ctctgcattt tgaccgcttg cagcggttta gcctatgcca atacagcaga
    13441 tttgccgcaa ggtgaaaggg tgcgtgtcgg gacggcaacg attgagcgca caaccgataa
    13501 aatgaccgtg aatcaatcca gcaataaagt acaaattgat tggaacagct ttgatattgg
    13561 taagaataaa gaagtggaat tcaaacagcc gacagagatg gcggtggcat ataaccgtgt
    13621 taccggcgga aacgccagcc aaattcaagg caagctgacc gcaaacggca aagtgtattt
    13681 agccaacccg aacggtgtac tcatcaccaa agatgcggaa gtgaatgtgg gcggtttgtt
    13741 agtgaccaca aaagatttag aaaaaatcag cgaaaacggc acaacaaaag ataaattcag
    13801 ccgcaaatta gcgaaagaag gcaaaattct taaccaaggt aaaattactg ccaaaggcga
    13861 gcgggctttt gtggtattgg tgggcgacga tgtggctaat gaaggggaaa ttaacgccga
    13921 aaattactac aaaaaagaga ccaaacgggt gagagaatgc tataacgacc cgtggagctg
    13981 ggggcaacaa tgctacgata ttgataaaga gatcgtaaaa agcaccagtg gcgaagtgca
    14041 tttatcctcc ggagaaaatg tcacctttac cttaagcgac cgctatattg atgtggcgtt
    14101 agacgataat acggtcaata gcattatcaa aaatgaaggt gccattattg ccaacggcga
    14161 cattactttt accgccaaag ggcgaaatca agccttggac agcttaatta tcaataatgg
    14221 cgtgttacaa gccaataagg tgcaaaataa aaacggcacc atcattttat ctgccggtga
    14281 aacccaatta cacgaaaaaa gccagattaa aggtgagaaa gtgagctttg ccgaagcttc
    14341 ctattacgac ggcccgaaag aaaacatcaa agtggtgtcc aaaacaggct caaaagtgac
    14401 cgcacctgtg atcgatttca aaggcaaaac cgtgaatatt caaggcagtt ttggtcgtga
    14461 agacagaaca gagcattaca atgatgaact caagacattg cgtacagtgg taaatattga
    14521 agtgccgaat acggaagata ttcgcattgc cgataccgac aacggcggta gcggttcctt
    14581 tatacaagcg ggtgctttag gctcattact tgcaaataac ggtcgagtga atgtacaggg
    14641 caaaggcttt gatgtctcag gcaagttgaa tgtgaatgcc tttaaacaaa cggattccgt
    14701 gctgaaattc atcaatcagg cggatatcaa tatccacaac gcagatattc actcaaacgg
    14761 gcgtttattt atgctgacta acctcccgaa tgacacggat ttccaatcaa atatccgcat
    14821 taccgacagc aaaatcaacc taggaaacgg tgcaatgggc tttggtcgca gtgcaaaacg
    14881 ttgggaaatg cacgatgtgg ttcgacgtgc cgatacggaa ctgcgtaaaa aattcaatct
    14941 ggatatgaaa aacgtggaat taaaccaagt ggatgatttt gtggtggcgg gcggtttcca
    15001 acaagttaat ttggataacg tgaaagcccg tggcaaaacc aacttctatc tggattccgg
    15061 caactcccgc gtgtttgtcg gtgagcagat ttatgaatat ggcgtaaccg atattgaaaa
    15121 acgcacccaa ttggcgaatt tagaccagcg tagaaaacgt tggaattcgg ataactatga
    15181 tgagatcgat ctcaactggg aatacaacca acgttttgac ggctttgcag caaaacacaa
    15241 tcaccgcgcg ccgattgccg gtacggaaat caatattgcc aatagtgata tcggcttaga
    15301 taacggtttt gtgcatttaa tggcagaaaa aatcaatctg gacgacagca aaatcaatat
    15361 caccttcaac caagacaaca gcttggatga aagtacccaa atcaaccgct taggactaaa
    15421 cggcaaagtg tcgatgaaaa acagccatat taacgtagtg ggcgatgaaa aagacggtat
    15481 ctcaccggct cgccattatg ccacgatgtt cttagtgggg gagctgcttg gtgaaaagag
    15541 ttctattttc ctcaaatccc accaaggtgc agtattgaga accgacagca atgttaaaat
    15601 tgccggtaaa aacagcaaag acgacctcaa aattaccgcc attaccaccg gtaaaaaaat
    15661 gggcaatgaa atcatcggac cggacggctt gccaacctcg gaagataacg atgccaacgc
    15721 cagcaacacc gcctttgcca tcggctatga acctaacgct acggctatcg tcgaaaatgc
    15781 aagcattacc gcattagcac caaacggcgg cttggcatac accggcacgt ctaaaaccca
    15841 aattgacgcg aaggataatg ctacctttac cttctttgaa aaacctcgag ccggctatga
    15901 caaattgctt ggcgacatca aaggtaaccc cgataaatta accccacgcg atttcgcccg
    15961 tttatacgac aacattaaag gcatcaaagc cagcagccta tccgaacaac agcttggcct
    16021 agcagacacc gcgaagaaaa ccagcgaaca caccttagca aacagcctag acgtagaaaa
    16081 tttggtttca gtgacagtgt gtgatgagca gaataagtgt gaggagcgta tgttaggtaa
    16141 caaaaatacc aatgctaaag ttattgtcgg agatatcaaa tttgagtagc gttgaagaaa
    16201 gtggtagaag ctcagacttg atctgataaa gcactctaaa aagtgagagt ttcccgttta
    16261 gaatatgagt gtcaaaattc aatctaaaca aaaaggaaac tctcatgttt tattacttac
    16321 aatcccctca ttaaacacaa gaccggttta ttaaatttag cagaagaacc gggcaatatc
    16381 tctcaagcct gcaatgtggt gggtatgagc ctagatacat tctattgcta tcaacaagcg
    16441 gttatttttg gcaactaatt tgcaaaaaaa caataaaatc cgaccgcttg tagagggtta
    16501 atccgccctt agtgaaattt caccgatgcc gaaatagcgg aggttgtcgc cctgttttag
    16561 aatgagttcg ccctgctcgt taatgccttg cgaaatgccg tggatttctt cggcttcggt
    16621 aatgagctta accgctttat ggcggaaaag gtcgaaactt tgccagcgtt cggcataatg
    16681 agcgaagccg acaagcggat aaattttgag atttttttgc aattcatagg ctaaacggca
    16741 aaccaattcg ttgcggttga aatgatattg cgataaatct gcccacgctt gcgtcacaat
    16801 attttcatcc acttttgcca ttcctaggtt cagcccaatg ccgataacca catctaaacc
    16861 attgcgattg gcttgggttt ccaacaaaat gccccccatt tttttgcctt ggtaataaat
    16921 gtcattcggc catttaattt gaatatcttt tacattttga gcagtaaggc tttccgcaat
    16981 gatcaaggct accactaaac ttaagggcgg taaatctgcc gcctgctcca tcgggtaatg
    17041 ccataaaatc gagaaataga gattttcact ctcgggcgaa taccaagttc gacctcgtct
    17101 gccacgtccg gcagtttgtt tttcagcgag gcaaaccgtg ccgttttcca aacgttggtg
    17161 atgagtgagc aaatattcat tggttgaatc aatttcatcg aacacaacgg cttgcccgca
    17221 aattaacgca ttttggattt gagcttggtt aagtttcatt atgtccccac ttagcgagtg
    17281 attttttcgc tatcgacctc gccgttcttg ccgataaaac gcacttcagg ctgaatttct
    17341 accccaaatt tttctctcac tctctggcga acggttttgg ctaacgccaa aacatcttgc
    17401 cctgttgcat tttctttgtt aatcagcact aaggcttgct gggtatgtac cgccgctccg
    17461 ccaatttgta agcctttcag cccggtttgg tcaatcaacc aacctgcggc taattttacc
    17521 gaaccattag cttgaggata gtgaggaatg gttgggaatt tggtttgtat tggtgcaaat
    17581 tgttcgtttg aaatcaccgg atttttaaaa aagctgccgg cattgccaaa ttcatcaggg
    17641 ttaggcaatt ttgaggagcg aaccgcacaa acttcatcaa aaatctgttt tggcgtaacg
    17701 gtttccggat tgagttgggc taatgatccg taacttagca ccggtttcca cgctttggct
    17761 agctttaagc ctaccgagat aatcgcaaat tcatcacggt attggtgttt aaacacactt
    17821 tcacggtagc caaattcgca ctcggatttg gatagcttaa agcgttcgcc ggaacgtaaa
    17881 tttaacactt ccacgaaatc acacagctgt tcaaattcca ccccgtaagc cccaatattt
    17941 tgaattggag cagagccggc gacacccgga attaacgcca gattttcaat gccggcaata
    18001 ttgtgtgcca acgtccattt aaccaattcg tgccagtttt ccccaccttg aacgtgcaaa
    18061 tagtggaaat gttcgtcttc gtggtgcgta atccctttga gtttatttac taacaccaca
    18121 ccgtcaaaat cctctaaaaa caaaacgtta gagccttgcc cgagaatcaa gattggcagt
    18181 tgagcgttaa aggctttttg ccattcgctc aatagctgtt cgactgtggt aaattcaata
    18241 atttgggtcg cttttgcagg caaatggaaa gtgtgaaaag gggttaagct atgtttcatc
    18301 atcaaattag ttagttaaga agtggaaata acaagcggtg atatttttac aaaattttgc
    18361 aattaagatt ttgggcaggc acaggatctg cccctacatt cagaattacc ttgccccata
    18421 taccacaatg gttttgccgt gagcagaaat taagccttcg tcttccaaca ttttcataat
    18481 gcgtccaacg gtttcacggg aacaaccgac catttgcccg atttcttggc gggtaatttt
    18541 gatctgcata ccttccgggt gagtcattgc atccggcatt ttagctaagt tgagcaaggt
    18601 ttgagcaatg cgaccggcaa cgtctaaaaa ggcaagatta ccgacttggc gtgaagtttg
    18661 acgtaaacgg cgagccattt gtgccgataa atacattaaa atatcggggt ttaaatggac
    18721 tagttgtttg aattttttat aggaaatttc cgcaatttca caaggatctt tagtgcggac
    18781 gtaagcggta cgttgttgga ctttttcttc aaataaactt aattcaccga caaactcacc
    18841 tgtgcctaag ttagttaaga gcatctcttt ggtttcatca tctttgatgt aaaccgacac
    18901 tgagccattc acgatgtaga aaagtgattc tgcatcttca ccggcgtgaa tgagtgtata
    18961 tttcgccgga taacgatgga tatgacaatg ggttaaaaac cactccaccg cagggtcgtg
    19021 aatagagggc ggaggagtta cattttccag aggagctgtt gaaagtgtca tatcttgcat
    19081 agtatttcct ataactctta tactaggtta aatttttaga attttaacac gaaattaaag
    19141 gttagcctaa aaaagatttt ttgtctttaa tatcaaggct ttcgtgtaat tccgaccaaa
    19201 caatcaccac tttatctgcc ttaagttgtg tgagtaatcg ttctcttttt tctgccagag
    19261 aaagttccct ttccccgtag tctgtgcctt ctcgtaaaat gaaggaatct aaaatattat
    19321 gtaaggttga ttcttcgagt tcttgccaag gtataatcat aattaatcaa ataaaggctt
    19381 aataaatgcc cactgttttt caaaatgttg gtgttggtta tagctgaaat tagaacgaac
    19441 caagcgtaaa aattgccctt cgcagaaatt cactaaataa ccggctaagg ctctttcgtc
    19501 ttcaaagctc ttgcgttcac gcagtttact aagttgcaaa atattggcaa attgtaactc
    19561 caagttatca aagaattttg ccactcgagc tttgagcagc tcctcttcaa acatcaaggc
    19621 gtgtccggtc aggatgcggg taacgcccgg gtttttgcga gcaaattcta aaatcacata
    19681 taaaattgct cgcaccgaat tggcactgct gtcttggcgt ttattggcgt gaatatagct
    19741 ggtgagggtc tgctcgattt tctcgattaa cgcttcaaac atcttggttt tacttgggaa
    19801 atagcgatac aacgccccct ccgacacccc caccgcagtg gctaaacgct cggtagtgat
    19861 acgctgcatt ccctcttcag aatttaacaa gctgattaac acttccaaca cttgttggcg
    19921 gcgttccacc actgattttt tcggcatttt aattttaggt tctgtcattg ccaatcctat
    19981 tattgcttac ctgaatgccc aaaaccgcct tcgccacggt cggtctgctc aaactcttgc
    20041 acaatgttaa aacttgcctg caccaccggc acaaagacta actgagcaat acggtcgccc
    20101 acgttcacgg taaaatcagc ttggctacgg ttccaaagcg acactttcag ctcgccttgg
    20161 taatcgctat cgatcaagcc gattaagttg cctagcacga tcccgttttt atgccctaaa
    20221 cctgatcttg gtaagatcac cgccgccaaa ttcggatcgg cgatataaat tgaaatcccc
    20281 gttgggatca acaccgtttc acccgctttt accgtcagcg gctcgttgag catcgcccgt
    20341 agatccaagc ctgccgagcc ttcggtcgca tacgcaggta atggaaattc attgccgata
    20401 cggctatcta aaatttttaa atcaatttgt ttcattcttt ttcctttttg taaattttta
    20461 aaactatatt attcagttat aaaaggtttt aattgtatcg ttccaagtgg
//
LOCUS       DANJWC010000025        20026 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.025, whole genome shotgun sequence.
ACCESSION   DANJWC010000025 DANJWC010000000
VERSION     DANJWC010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20026
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.025"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..328
                     /locus_tag="PWX02_000688"
     CDS             <1..328
                     /locus_tag="PWX02_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728724.1"
                     /translation="DEFTFIKTFASAHKLTLQNFVMSAIQAKVAEMSAKNKISEESNI
                     HQPLSKDEKIMLNEAKVFYSARSHLKAENVILPSDFMEQIKSGCGAEKMAEMMGYTKE
                     DWESMK"
     gene            330..653
                     /locus_tag="PWX02_000689"
     CDS             330..653
                     /locus_tag="PWX02_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728725.1"
                     /translation="MGQITFSNIFTKVIFENVLSASTKEKIKQMLFQSVVALPPLHSE
                     RKMILQLKKQKITIFYQVIEEQSVQILAYQFGGTDKLTGVSKAHFKTLDAIFQMTDEE
                     KEGKF"
     gene            complement(663..995)
                     /locus_tag="PWX02_000690"
     CDS             complement(663..995)
                     /locus_tag="PWX02_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL1728726.1"
                     /translation="MQIEQYQQWVRQFYQQQGWYERSPFMRVTYLAEEIGEVACAVRA
                     IEIGRERPDETEASQQQKRDNLIEELGDVMDNLFVLADKYGISMTEVLERHQAKFEKR
                     YLQKNMEK"
     gene            complement(1088..2284)
                     /locus_tag="PWX02_000691"
     CDS             complement(1088..2284)
                     /locus_tag="PWX02_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDL1728727.1"
                     /translation="MNIPFCLPENISPETFLREYWQKRPLLIRNGLPQIVGLFEPEDI
                     LELALEEEVTARLIQCENEKWSVKSSPLVEEDLEELPVQWSVMVQNLEQWSPELGELW
                     QAFGFIPQWQRDDIMVSCSPLNGTVGKHYDEYDVFLVQGYGQRRWQLGKWCDPSTEFK
                     PNQPIRIFDDMGELVMDEVMNPGDVLYVPSRMAHYGVSESEQGLTFSFGLRYPNATDL
                     LENLCKTIEQQADNINSSEFHLPFRLTPNEQPNALLDPKMVKMFKHQLIDLLQNSEQF
                     DELLTHSVATAASSRRYELLQTDNEYYPDEVQEILEAGGWLQQDANVKILYTENPQRV
                     YVNGEWIDELNEAEMALLIRIANGDSISWNKLASKVQDQAELELMLDTICDWLDSGWI
                     ILSEAE"
     gene            complement(2433..3776)
                     /gene="accC"
                     /locus_tag="PWX02_000692"
     CDS             complement(2433..3776)
                     /gene="accC"
                     /locus_tag="PWX02_000692"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDL1728728.1"
                     /translation="MLEKVVIANRGEIALRILRACKELGIKTVAVHSTADRELKHVKL
                     ADETICIGPAPSVKSYLNIPMIIAAAEVTNADAIHPGYGFLSENANFAEQVEKSGFIF
                     IGPTADVIRLMGDKVSAINAMKKAGVPCVPGSDGPVGNDPKKNKEIAKRIGYPVIIKA
                     SGGGGGRGMRVVHHEKDLEESIAMTKAEAKAAFNNDMVYMEKYLENPRHIEIQVLADT
                     HGNAVYLAERDCSMQRRHQKVVEEAPAPGITPELRQFIGERCANACREIGYRGAGTFE
                     FLYENGEFYFIEMNTRLQVEHPVTEMITGVDLVKEQLRVAAGLPLSITQDDIKVKGHA
                     IECRINAEDPNTFLPSPGKITRLHVPSGLGVRWDSHIYSGYSVPPHYDSMIGKLITFA
                     ENRDIAIRRMENALSETIVEGIKTNIPLHTQILADKEFRKGGTNIHYLEKKLGLK"
     gene            complement(3848..4315)
                     /locus_tag="PWX02_000693"
     CDS             complement(3848..4315)
                     /locus_tag="PWX02_000693"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDL1728729.1"
                     /translation="MDIRKIKKLIELVEESGITELEVSEEEGTVRISRAAPAVAPAAV
                     QYAAAPVVAPAAPQVASTAPVAAPAEAPKAAETSGHAVLSPMVGTFYRSPSPDAKPFV
                     EVGQTVKIGDALCIVEAMKMMNRIESDKAGVVKEILVNDGEPVEFDQKLFIIE"
     gene            complement(4568..5350)
                     /locus_tag="PWX02_000694"
     CDS             complement(4568..5350)
                     /locus_tag="PWX02_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL1728730.1"
                     /translation="MAVKNEQFIVICNGVFRLFGNIVKILSYPFHALFPKKRFTIPEY
                     SPAKIQSSIPSKITKTIWQTNYSNKVTLPMYANYLFTRLMSLSYDYRYVSTEEREAYI
                     KANADERTFNAYSKLTDGAAQADFWRIFTLLREGGIYIDIDGHLVYPLSKIIGENDSE
                     VLIKRRDKYTNFFLASEAGNWILKDTLELIISNIENRRIEGGVFVMTGPDTLNNAIGD
                     KEVNFRRDKVTCAQGTFTNEYFQYIDKPGSKWNYKKNEDLLK"
     gene            complement(5360..5797)
                     /gene="aroQ"
                     /locus_tag="PWX02_000695"
     CDS             complement(5360..5797)
                     /gene="aroQ"
                     /locus_tag="PWX02_000695"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HDL1728731.1"
                     /translation="MKKILLLNGPNLNMLGKREPQIYGSQTLADIENHLKKLAAAQDV
                     ELESFQANGEEPIINRIHQAFQHTDFIIINPGAFTHTSVAIRDALLSVSIPFVEVHLS
                     NVHSREPFRHHSYLSDIAKGVICGLGAKGYEYALDFAISHLKA"
     gene            complement(5804..6262)
                     /locus_tag="PWX02_000696"
     CDS             complement(5804..6262)
                     /locus_tag="PWX02_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1728732.1"
                     /translation="MAFTIRKMVEQDTHQVFPLMQKLAVFEHYIDSFAITPEVVMESG
                     FRKSPPDFYCLVAENASNIIGILVYYFLPYTAQNRPAIYMKELYVDETARNQKVGEAL
                     MQALKNEAKAHHCKTIKWTVAPWNDGGIRFYQRLGAKENRKWLNYELQVE"
     gene            complement(6322..7146)
                     /gene="dapD"
                     /locus_tag="PWX02_000697"
     CDS             complement(6322..7146)
                     /gene="dapD"
                     /locus_tag="PWX02_000697"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HDL1728733.1"
                     /translation="MSLQAIIEAAFERRAEITPKTVDAETKAAVEEVIAGLDNGSLRV
                     AEKKDGEWVVNQWVKKAVLLSFRIADNEIIDGAETKFYDKVPTKYGNYTEEQFKADGI
                     RAVPGAVVRKGSHIEKNVVLMPSFVNIGAYVGEGTMVDTWVTVGSCAQIGKNVHLSGG
                     VGIGGVLEPLQANPTIIGDNCFIGARSEIVEGVIVEDNCVISMGVFIGQSTKIYDRET
                     GEVHYGRVPAGSVVVSGSLPSKDGSHNLYCAVIVKKVDEKTLSKVGLNDLLRSIDE"
     gene            7294..7956
                     /locus_tag="PWX02_000698"
     CDS             7294..7956
                     /locus_tag="PWX02_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDL1728734.1"
                     /translation="MENFANLLTGKTREHLVPLPNALSDKHYLQPQVVEAFLTLQHAA
                     KQAGFNLQPASTFRDFERQKGIWNAKFRGERKVHDDNGVAIDMAALSDLEKCKAMLRW
                     SAVPGASRHHWGTEIDIFDPDLLPENTPLLLEPWEYLEGGYFADLTKWLHVNAETFGF
                     YFPFDGKHSRVGFEPWHISYRPISNEYEQRFNDEILKAAWQDEEVAGKECLLEHFEEL
                     LK"
     gene            7953..8705
                     /locus_tag="PWX02_000699"
     CDS             7953..8705
                     /locus_tag="PWX02_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL1728735.1"
                     /translation="MIQLINETQQHDKFRQICEKWQLVEDKSSPLALVLTEERLELRK
                     LDEPKLGAIAVNFVDGTMAHRRKFGGGRGEAVAKAVGIKGDYLPTVIDATAGLGRDAF
                     VLAAVGCKVLLVERNPIVAALLEDGLNRAYQDVEIGSFMQERMILADVRDISQLDPQS
                     QMAEVVYLDPMYPHKQKSALVKKEMRVFQHLVGADLDSDSFFLPAKGLAKKRVVVKRP
                     DYAPFLAEQKPDFSHTTKNHRFDVYLSHLKND"
     gene            8836..9672
                     /gene="ftsN"
                     /locus_tag="PWX02_000700"
     CDS             8836..9672
                     /gene="ftsN"
                     /locus_tag="PWX02_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HDL1728736.1"
                     /translation="MPQRDYVARSNEKKSKKNQKESNVKKILMRIIGVILFFGIGLWF
                     LKSSAPVPTVPSAPQPANSQTKSELPSRPEETWSYIRDLETRTVITDNSQTSQERLAQ
                     LTAEQKQQIEERRKQEEARLAAEQAQQKPATDVSATELAPKTEEVKSLTEEELAAKKA
                     QEQALAQKQAEEKRRQAELKKQQEAKKEQPAKIVASAATLADKAANQAGKFGLQCGAF
                     KNKAQAENMQARLAMAGFNARIASSADWNRVFVGPIGDRAATSRAQSNARSVAECVIV
                     AM"
     gene            complement(9714..10895)
                     /locus_tag="PWX02_000701"
     CDS             complement(9714..10895)
                     /locus_tag="PWX02_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HDL1728737.1"
                     /translation="MKTELDMIVIGGGMIGAATALGLAKQGLRLALLEKNPLPTFNPD
                     SDYDLRISAISMGSIQLLEKLGVWEQIVAMRSTPYTQLETWEIDGFNTLFSAADLGLE
                     KLGDMVENNVIQIALWEQLKQYPNCLQAVGFEQIFANKAGEYWEVTVDGNTFAAPLLI
                     AADGANSQVRQWAGIGLTAWQYRQHCLLATVTTEKPAPSGTWQQFYPTGPRAFLPLSG
                     NHGCVVWYDSPQKIAELKMLPAEKLGVAIEQHFPSRLNAELGNIQVQASGAFPLTRQH
                     AQHYVQNGVVLVGDAAHTINPLAGQGVNLGFKDVKTLLEVIEQAVKKGKNFASEQVLQ
                     RYEQKRKPDNLLMQTGMDVFYKAFKTDLLPLKIARNVGLVLADKVSPIKKQALKYAIG
                     I"
     gene            11002..11337
                     /locus_tag="PWX02_000702"
     CDS             11002..11337
                     /locus_tag="PWX02_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728738.1"
                     /translation="MSCKTHANHEHEHGEGCGHTAIKHNDHIDYLHDGHLHHKHGDHY
                     DEHVLEVNETNPADCNAAHCCGDHVHGEGCGHEAVPHGDHIDYIVNGRLHHQHGDHCD
                     DHGPVEIVK"
     gene            11469..13001
                     /locus_tag="PWX02_000703"
     CDS             11469..13001
                     /locus_tag="PWX02_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDL1728739.1"
                     /translation="MTLAIVYSRASIGVEAPLVTIEVHISNGQPKLTIVGLPEATVKE
                     AGDRVRSALLNANFIYPPQRITINLAPADLPKEGGRFDLPIAIGILAASGQIDSDLLT
                     QFEFLGELALTGHLRGVHGTIPAVISADKAKRQMILAKQNANEASLVSNATTYFAGSL
                     LEVVNMLNKRDKLPICQHISQHSAEIRPLVSRDLTDIIGQQHAKRALMIAAAGQHNLL
                     FLGPPGTGKTMLASRLADLLPEMTDEEAIETASVTSLVQNELNFQNWKQRPFRSPHHS
                     ASMVALVGGGSIPKPGEISLAHNGVLFLDELPEFERKVLDALRQPLEAGEIVISRANA
                     KVQFPARFQLIAAMNPSPTGHYQGTHNRTSPQQVMRYLNRLSGPFLDRFDLSIEVPLL
                     PQGALQSGTENRGETTEQVRKRVFLARKMQFERAGKINSQLTTREIERDCQLSAQDAL
                     FLETALTKLGLSVRAYHRILKVSRTIADLAGEANIQQLHLAEALGYRAMDRLLQRLQG
                     EP"
     gene            complement(13050..13889)
                     /gene="menH"
                     /locus_tag="PWX02_000704"
     CDS             complement(13050..13889)
                     /gene="menH"
                     /locus_tag="PWX02_000704"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDL1728740.1"
                     /translation="MLSYQWHAQQGTPVIFLHGLLGSQQDWQAVLQHLQNFPQIRPLT
                     IDLPFHGNSKHITCADFADLRQQLHATLESLIGSRPFYLVGYSLGGRAALDYVLNMDN
                     PNLQGVVLEGANIGLSTENDRQIRRQNDRHWANRFRQEPIEQVLEDWYKQPVFADLSD
                     TERECYIDSRKHNQGEQIAQMLEATSLAKQPDYTEKLKQTDKNIVFFIGEKDQKFRKM
                     ASSHQLPTQLIANAGHNAHRANAKNFVEKLGLLILHSDSDLRSQSVKHNGAALLHKSP
                     SGA"
     gene            complement(13938..15644)
                     /locus_tag="PWX02_000705"
     CDS             complement(13938..15644)
                     /locus_tag="PWX02_000705"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDL1728741.1"
                     /translation="MSVSSFNRTWAKVILSALMRYGVKHFCIAPGSRSTPLTLEAVQL
                     QQTQYAECHTHFDERGLGFYALGIAKATNSPVAVIVTSGTAVANLYPAVIEASLSHQN
                     LIILSADRPTELIGCGANQAITQQGIFANYPAASINLPKANEAYNASWLVSTIEQACT
                     TQAQQGGVLHINAPFEEPLYEADINEILNTPWLRPIQGWLNNSQTKWIDQQAIQSEVS
                     MHENWDYWRTKRGVIVVGKLPPEQGTGLKNWAETMGWCLLSDVQSCIEASLPYADIWL
                     SNETVKQRLLQADIIIQFGTQIVSKRVNEFLKAYQGEFWLVDESQDYINPNAHHQTRF
                     VAKAHHFLRVHPPLRQKPWLLEPLALSQFCATFIEQQVGGSLSEASLAHNIQDILAIN
                     GNLFIGNSLFVRLVDALCKLPEGYPVYTNRGASGIDGLIATMAGIAKGSDRPTVGVVG
                     DISALHDLNSIALLTKINQPTILFVVNNSGGAIFDMLPVESKAKDQYYRLAHNFEFAQ
                     VATMFGLEYIRPFTWADLKTKLKHAYARKGVTIVEIKVNEHDGSNMYKSLLKQISQAE
                     IA"
     gene            complement(15825..17108)
                     /gene="dctM"
                     /locus_tag="PWX02_000706"
     CDS             complement(15825..17108)
                     /gene="dctM"
                     /locus_tag="PWX02_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003105529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter large permease
                     protein DctM"
                     /protein_id="HDL1728742.1"
                     /translation="MIILVLFSLLFILMFLGVPIAISLGLAGAVTIMFFSQDSVSSLA
                     IKLFETSEHYTLLAIPFFLLAGAFMTTGGVAKRLIDFANATVGHIRGGLAIAAVMSCM
                     LFAALSGSSPATVAAVGSIAIAGMVRSGYPVGFGTGIICNAGTLGILIPPSVVMVVYA
                     ASTETSVGKLFMAGVVPGLMLGLVLMIAIYVVARIKNLPAQPRATVKEWFTAARKAIW
                     GLLLMVIILGGIYSGIFTPTEAAAVAAVYSWFVAVFIYKDVKLRDCPKVLLESAKLSV
                     MLMFIIANAMLFAHVLTTEQIPQLITNTVVEWNLQPWTFLLVVNIVLLIAGAFMEPSA
                     IILILAPILFPIAVQLGIDPIHLGIIMVVNMEIGLITPPIGLNLFVASAVSKMPLSDV
                     IKAAMPWLLLLLGFLVLITYIPAISMALPNWIGIN"
     gene            complement(17108..17731)
                     /locus_tag="PWX02_000707"
     CDS             complement(17108..17731)
                     /locus_tag="PWX02_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003105527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL1728743.1"
                     /translation="MSSLKWFWERFEECFIAILLMVMTLVTFTYVMFNNLYNVFFDLA
                     EAVPFTASFAEPLGEWIMEMAQEMTWSVALTKVCFGWLIFFGASYGVRTAGHIGVDVL
                     VKKLSRNAQKVVGLIAIAACLAYAFLIASASYEWISAMYVADIGAEDLHQFHLKQWHV
                     GLIMPVGFALIGIRFIEILVRILQGKQVGLGLADESEDAMKLAQGEE"
     gene            17955..18902
                     /locus_tag="PWX02_000708"
                     /pseudo
     CDS             17955..18902
                     /locus_tag="PWX02_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012621529.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(19039..20013)
                     /locus_tag="PWX02_000709"
     CDS             complement(19039..20013)
                     /locus_tag="PWX02_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL1728744.1"
                     /translation="MAAALAVTAAFSVSAFANDPIVIKFSHVVANETPKGQGALMFQK
                     LVDERLNGKVKVEVYPNSSLYADGKEMEALLLNNVQMLAPSLAKFDKYTPKIQIFDLP
                     FLFDDIKAVERFEQSEAGQSLLKSMEDKGITGLSYWNNDLKQLSANKELREPKDARGL
                     KFRVQASEVLDAQFKALKAVPRKMAFGEMYQGLQTGVVNGAENPYSNIYSQKIHEVQK
                     FITASNHGLLSYMVIVNTDFWNKIPEDVRTELTKILDEVSVEVNKQSHDLNQSDKQKI
                     VDSGASQIITLTDEQREKWREAMRPVWKQFEDAIGADLIKAAEAANKQ"
BASE COUNT         5690 a         4352 c         4361 g         5623 t
ORIGIN      
        1 cgatgaattt acttttatta aaacatttgc ttctgctcat aaactgactt tacaaaattt
       61 tgtgatgtcc gcaatccaag ctaaagttgc agaaatgtct gctaaaaata aaatatctga
      121 agaaagtaac attcatcaac ctctttctaa agatgaaaaa ataatgctaa atgaggcaaa
      181 agtattttat tctgctcgtt cgcatttaaa ggcagaaaat gtgattcttc cgagtgattt
      241 tatggagcaa attaaatcag gctgtggagc agaaaaaatg gcagaaatga tgggatatac
      301 caaagaagat tgggagtcaa tgaagtaata tggggcagat tacatttagt aatattttca
      361 caaaagtgat ttttgagaat gtgctttctg cttcaactaa agagaaaata aaacaaatgc
      421 tttttcagtc tgtggttgca ttacctcctc tccattcaga aagaaaaatg attttacagt
      481 tgaaaaagca aaaaattacc attttttatc aagttattga ggaacaatct gtgcaaatcc
      541 tcgcttatca atttggtgga actgataaat tgacaggtgt gtctaaagct cactttaaaa
      601 cactagatgc catttttcaa atgacggatg aagagaaaga aggaaaattc taatgtaagc
      661 ggttattttt ccatattttt ttgcaaataa cgtttctcaa attttgcttg atggcgttca
      721 agcacttccg tcatcgaaat gccatattta tcggctaata caaaaagatt atccatcaca
      781 tcgcctaact cttcgattaa gttatctcgt ttttgctgtt gtgaagcttc ggtttcgtcc
      841 ggtcgttcac gcccgatttc aatcgctctt actgcacaag ccacttcgcc gatttcttct
      901 gctagataag tcacccgcat aaaagggctt cgttcatacc aaccttgttg ttggtaaaac
      961 tggcgaaccc attgttgata ttgttcaatt tgcattttat ttctgattca aaaagtaggg
     1021 acgaaaaatt tttcgccctt ttataagaaa atgtatcagg gcaaatgatg atttgcccct
     1081 aggagtgtta ctctgcctca gagagaataa tccagccact atccagccag tcgcaaatgg
     1141 tgtctagcat tagctccagc tccgcttggt cttgcacttt ggaggcaagt ttgttccacg
     1201 aaatcgaatc tccattggcg atacgaatca gtaatgccat ttctgcttcg tttaactcat
     1261 caatccactc gccatttaca taaacccgtt gagggttctc ggtgtagagg atttttacat
     1321 ttgcatcttg ctgtaaccag ccgccggctt cgagaatttc ttgcacttcg tcagggtaat
     1381 attcattgtc agtttgcaat aattcataac ggcgggagct tgccgcagtg gcaacactgt
     1441 gagtgagtaa ctcatcgaac tgttcggaat tttggagcaa gtcgattaac tgatgtttaa
     1501 acattttcac cattttcgga tcaagcaacg cattcggctg ttcattcggc gttaagcgga
     1561 acggaagatg aaattccgat gaattaatgt tgtcggcttg ttgttcgatg gttttgcaga
     1621 gattttccag taaatcggtc gcatttggat aacgtaaacc aaaagagaaa gttaaacctt
     1681 gctcgctttc ggaaacacca tagtgagcca ttcgagacgg aacataaagc acatcacccg
     1741 ggttcattac ctcgtccatt accagttcgc ccatatcatc aaaaatgcgg atcggttggt
     1801 ttggtttgaa ttcggtgctt ggatcgcacc atttgcctaa ttgccaacga cgttgcccat
     1861 agccttgcac caagaacaca tcgtactcat cataatgttt gccgacagtg ccgttgagcg
     1921 gtgagcaaga aaccataatg tcatcacgtt gccattgcgg gataaaacca aaagcctgcc
     1981 aaagctcgcc tagctcagga gaccattgct cgaggttttg caccattaca gaccattgta
     2041 caggtaactc ttccaaatct tcctcaacaa gcgggctgga tttaacagac catttctcgt
     2101 tttcgcattg aatcaggcgt gcagtgactt cttcctctaa tgccagttct aaaatatctt
     2161 ccggctcaaa caatccgaca atttggggta agccgttacg aattaatagc ggtcgttttt
     2221 gccaatattc tcgcaaaaag gtttcagggc tgatattttc aggtaagcag aatggaatgt
     2281 tcataatggc tccttgctac gtgcttcttt gagattactg atattctacc cgagaaatca
     2341 ggaaaaacgg gaaatttttt atgatttagc gacatcattc acattttgaa gaggaaataa
     2401 tgaaaaaggc gaacattgtt cgcctttgaa atttatttta aaccgagttt tttctcgaga
     2461 tagtggatat tggtgccacc tttgcggaat tctttatccg caagaatttg agtatgtaaa
     2521 ggaatattgg ttttaatgcc ttccacaatg gtttcggaaa gggcattttc catccggcga
     2581 atcgcaatat cacgattttc agcaaaggta atcaatttac caatcattga atcgtaatgt
     2641 ggcggcacgc tatagcctga gtaaatatgt gaatcccaac gcacacctaa accactcgga
     2701 acgtgtaaac gggtaatttt acccggcgat ggcaagaagg tgtttggatc ttctgcgttg
     2761 atacggcact caatggcgtg acctttcact ttaatatcat cttgtgtgat cgaaagtggt
     2821 aagccggctg ccacacgcag ttgctctttt accaagtcca caccggtaat catttcggtt
     2881 acagggtgtt ctacctgtaa gcgggtgttc atttcaatga agtaaaactc gccgttttca
     2941 tataagaact caaaagtacc tgcaccacgg tagccaattt cacggcaggc attggcacaa
     3001 cgctccccaa taaattgacg caattcaggt gtaatacccg gagcaggtgc ttcttccacc
     3061 actttttggt ggcgacgttg cattgaacag tcacgctctg ctaaataaac cgcattaccg
     3121 tgagtatcgg caagtacttg gatttcaata tggcgtggat tttctaaata tttctccata
     3181 tacaccatat cgttattaaa tgccgctttc gcttcggctt tcgtcattgc gatagattct
     3241 tccaaatctt tctcgtgatg aaccacacgc ataccacgac cgccaccacc acctgatgct
     3301 ttgatgatca ccgggtagcc gatacgttta gcgatttctt tatttttctt cggatcgtta
     3361 ccaaccggac cgtcagaacc cggtacgcaa ggcacgccgg cttttttcat tgcattgata
     3421 gcagagactt tatcgcccat taagcgaatc acatcagccg ttggaccaat aaagataaag
     3481 cctgatttct caacttgttc ggcaaagttt gcgttttcag ataagaaacc gtagcccgga
     3541 tgaatagcat cggcattcgt tacttcggca gcggcaataa tcatcgggat atttaaatag
     3601 ctttttaccg atggagcagg accgatacaa atggtttcat cggctaattt aacgtgtttt
     3661 aattcacggt cggcagttga atgcactgca accgttttaa tgcccaattc tttacaagca
     3721 cgcaaaatac gcagggcaat ttcaccacgg tttgcgataa caactttttc taacataggg
     3781 aaattccttg tgttctagaa aatgtagggc gtgttgcaca cgcccgttgg ataatttaag
     3841 gaagagctta ttcaatgatg aataattttt ggtcgaattc taccggttcg ccatcattaa
     3901 ctaagatttc ttttaccaca ccggctttgt ctgactcgat acggttcatc attttcattg
     3961 cttctacaat acataacgca tcaccgattt tcacggtttg acccacttca acgaacggtt
     4021 ttgcatctgg gcttgggcta cggtagaaag tccccaccat tggtgaaagc actgcgtggc
     4081 cactcgtttc tgcggctttt ggtgcttcag ccggagctgc aacaggtgct gtcgaagcta
     4141 cttgaggtgc cgctggtgca acaacaggag ctgctgcata ttgaactgca gctggtgcaa
     4201 cagctggagc agcacggcta atacgcaccg taccttcttc ttcagacact tctaactcgg
     4261 taatgccaga ttcttcaact aattcaataa gttttttgat tttgcgaata tccataataa
     4321 gattccgtat gtgattaaat aaaataaaaa agagaactcc acatttgggg tataaattca
     4381 aatgtagagt taaaaaatgc tgggcgtaga atacctgaat ttcgctaatt ttgcgaataa
     4441 aatctgataa ttttggcgaa aatggacgat tttagcattc atcgtaccag ttaggatttt
     4501 tggaaaatgg tgactcacaa gcgattgaaa attgccaatt ttttgtaaaa aacaaccgct
     4561 tgtcagatta tttcaataaa tcttcatttt tcttgtaatt ccacttgcta ccgggcttgt
     4621 cgatatactg aaaatattcg ttggtaaacg taccttgagc acaagttact ttgtctcggc
     4681 ggaaatttac ttctttatca ccaatggcat tatttagggt gtcaggaccg gtcataacaa
     4741 acacgccacc ttcaatccga cgattctcaa tattgctgat aatcagctca agggtgtctt
     4801 ttagaatcca atttccagct tccgaagcca agaaaaagtt ggtgtattta tcacggcgtt
     4861 taattaacac ttcgctatca ttttcgccaa taattttaga gagtgggtaa accaaatgcc
     4921 cgtcaatatc aatataaatg ccaccttctc ggagcaaagt aaaaattcgc caaaaatcag
     4981 cttgtgccgc accatcggtt aatttgctat aggcgttaaa ggtgcgttca tcggcgttgg
     5041 ccttgatgta tgcctctcgt tcttctgtgc taacgtagcg ataatcgtag ctcaatgaca
     5101 ttaggcgggt aaacagataa ttcgcataca ttggcaaggt gactttgtta gaataattcg
     5161 tttgccaaat ggttttggta attttgctcg gaatgcttga ttggattttt gccgggctat
     5221 attccggaat ggtgaaacgt tttttcggaa aaagtgcatg gaacggataa ctcaatattt
     5281 tgacgatatt accgaataaa cggaagactc cgttacagat cacaataaat tgttcatttt
     5341 taacagccat tttattacct tatgctttta agtgtgaaat tgcaaaatcc aacgcatatt
     5401 catagccttt tgcccctaaa ccgcaaataa cgccttttgc aatatcactt agataggagt
     5461 gatggcgaaa aggttcacgg ctatgaacgt tagacaaatg cacctcaaca aacggaatcg
     5521 acacactcaa taacgcatca cgaattgcca cgctggtgtg agtaaatgcc ccggggttaa
     5581 taataataaa atcggtatgc tgaaaggctt ggtgaatacg gttgataatc ggctcttcac
     5641 cgttggcttg aaagctctcc agctcaacat cttgggccgc agccaatttt tttaagtggt
     5701 tttcaatatc cgccaaagtt tgcgaaccgt aaatttgtgg ctcacgtttg cccaacatat
     5761 ttaaattcgg gccgttaagg agaaggattt ttttcatatt tacttattca acttgtaatt
     5821 cataattaag ccatttacgg ttttctttcg cccccaagcg ttgataaaaa cgaataccac
     5881 cgtcattcca aggagcaact gtccatttta ttgtcttaca atgatgtgcc ttcgcctcat
     5941 ttttcaaggc ttgcattaac gcctctccca ctttctgatt ccgagcagtt tcatcaacat
     6001 aaagctcttt catataaata gcggggcggt tttgggcagt gtaaggaaga aaataataaa
     6061 ccaatatgcc gataatgtta gacgcatttt cagccactaa acagtaaaaa tcgggtgggc
     6121 ttttgcgaaa gccggattcc attacaactt caggagtaat cgcaaaagaa tcgatatagt
     6181 gctcaaacac agccagtttt tgcatcagag ggaacacttg gtgggtatct tgttcgacca
     6241 ttttgcggat tgtaaatgcc ataaaattcc ttacaagcgg taaaaaaatc cctcactttt
     6301 gcaaagcgag ggaggtttta attattcgtc aatagaacgt aataagtcgt ttaagcccac
     6361 tttgcttaag gttttctcat ccactttttt cacaatcacc gcacagtaga ggttgtgtga
     6421 accatctttt gatggaagcg agcctgaaac cacaacagag cctgccggca cacggccgta
     6481 atgtacttcg ccggtttcac ggtcgtaaat tttggtagat tgaccgataa acacgcccat
     6541 tgagatcacg cagttatctt caacaatcac gccttctacg atttcagaac gtgcaccgat
     6601 aaagcagttg tcgccgatga tggttgggtt cgcttgtaat ggctctaaca caccaccgat
     6661 gcccacgcca ccagaaaggt gaacattttt accgatttgc gcacaagaac ccactgttac
     6721 ccaagtgtcc accattgtgc cttcgccaac gtatgcaccg atgttcacga aagatggcat
     6781 taacaccacg tttttctcga tatgtgaacc tttacgcacc accgcacccg gcacggcacg
     6841 aatgccatct gctttgaatt gctcttcggt gtagttgccg tatttggttg gcactttgtc
     6901 gtagaatttg gtttctgcac cgtcgatgat ttcattgtcg gcaatacgga atgaaagtaa
     6961 taccgctttt ttcacccatt ggtttactac ccactcgcca tcttttttct cggcaacacg
     7021 cagtgaaccg ttgtctaatc ctgcgatcac ctcttctact gctgctttgg tttcagcatc
     7081 aacggttttc ggagtaattt ccgcacggcg ttcaaatgcc gcttcaataa tagcttgtaa
     7141 agacattgtt gtgttccttc aatatataaa ttaaaacaga aaattcggct cactttttaa
     7201 catagaaagg cgtttatttc cacaagaaaa aacatatcgc agaaatttag tttataattc
     7261 caagccactt atttgataca agcggtgatt tttgtggaaa attttgcaaa tctgcttacc
     7321 ggtaaaacgc gtgagcattt agtgccgttg ccgaacgctc tttcggataa acattacctt
     7381 cagcctcagg tggttgaggc ttttttgacg ttgcagcatg ctgcaaaaca agccggcttt
     7441 aatttacagc ctgccagtac ttttcgtgat tttgaacgcc aaaaagggat ttggaatgcg
     7501 aagtttaggg gtgagcgtaa ggttcacgat gataacgggg ttgcgattga tatggcggcg
     7561 ttatcggatc ttgaaaagtg caaagcaatg ttgagatggt ctgcggtgcc gggggcaagt
     7621 cggcatcatt ggggaacgga aattgatatt tttgaccctg atttattgcc ggaaaatacg
     7681 cctttattgc ttgagccgtg ggaatatttg gaaggtggct attttgccga tttaaccaag
     7741 tggttacacg ttaatgcgga aacatttggc ttttatttcc cgtttgacgg taaacattct
     7801 cgagtgggct ttgagccgtg gcacattagc tatcgcccga tttcaaatga atatgagcaa
     7861 cgctttaacg atgaaatctt aaaagctgcg tggcaagatg aagaagtggc gggtaaggaa
     7921 tgcttacttg aacattttga agaattacta aaatgatcca attaattaac gaaacccaac
     7981 aacacgacaa attccgccaa atttgcgaaa aatggcagct ggttgaagat aaatccagcc
     8041 cgttagcctt agtgctaacg gaggaacgat tggaactacg caaattagac gaacccaagc
     8101 tcggggcgat tgcagtgaat tttgtggacg gtacgatggc acatcggcgt aaattcggtg
     8161 gtgggcgagg tgaagccgtt gcgaaagcgg tcggcattaa aggggattat ttgccaactg
     8221 tgattgatgc gacagccggt ttagggcgag atgcttttgt attagcagcg gttggttgta
     8281 aagtgctgtt agtggaacgc aatccaattg tcgctgcctt gttggaagat ggtttaaacc
     8341 gagcttatca agatgtggaa atcggcagtt ttatgcaaga aagaatgatc ttagccgatg
     8401 ttcgagacat ttctcagcta gatccacaat cccaaatggc tgaggtggtg tatttagatc
     8461 cgatgtatcc gcacaaacaa aaaagtgcgt tggtgaaaaa agaaatgcgg gtgtttcaac
     8521 atttagttgg ggcagattta gattcggaca gctttttctt accggcaaaa ggattggcaa
     8581 aaaaacgtgt ggtagtgaag cgtccggact atgccccgtt tttagcggag caaaagcctg
     8641 atttttcaca caccaccaaa aatcaccgtt ttgatgttta tctttctcac ctgaaaaatg
     8701 attaaaggtg ggtcaggatc ggtttttaaa aaactttaac ttgaaaagct gcctatgcga
     8761 taggcaagcg gttattttta cagtaaaatt tacaaattat gttatcgttt atttatattt
     8821 aaaaaggcgg ttatcgtgcc acaacgtgat tatgtcgctc gttccaacga gaaaaaaagt
     8881 aaaaaaaatc aaaaagaaag taatgttaaa aagattttaa tgcggattat cggggtaatt
     8941 ttattcttcg gtattggttt atggtttttg aaatccagtg cacctgtacc aacagttccg
     9001 tcagcaccgc agcctgctaa ttcacagact aaaagcgaat taccaagtcg accggaagag
     9061 acgtggagct atattcgtga tttagaaacc cgtacggtaa ttaccgataa tagccaaact
     9121 tcacaagaac gtttggcaca attaacggcg gaacaaaagc agcaaattga agaaagacgc
     9181 aaacaagaag aagcacgctt agccgctgag caagctcaac aaaagcctgc gaccgatgta
     9241 tctgctacag aattggctcc gaaaacagag gaagttaagt cgttaacgga agaagaatta
     9301 gcagcaaaaa aagcacagga gcaagcttta gcacaaaagc aagcggaaga gaaacgcaga
     9361 caagcagagc ttaaaaaaca gcaggaagcg aaaaaagagc agccggctaa aattgtagca
     9421 agtgctgcaa cactggcaga taaagctgca aatcaggcgg gtaaatttgg gttacagtgc
     9481 ggtgcattta aaaataaagc ccaagccgaa aatatgcaag caagattggc aatggcaggt
     9541 tttaacgccc gtatcgcaag cagtgccgat tggaaccgtg tttttgtcgg cccgataggt
     9601 gacagagcgg caacttccag agctcaatcc aatgcacgca gtgtagcgga atgtgtgatt
     9661 gtggcgatgt aaaattgatt gaaatattga agagagctat aggctctctt tttttatatc
     9721 ccaatcgcat atttcaaggc ttgctttttg atgggtgaca ctttatcagc caatactaaa
     9781 ccgacattgc gggcaatttt gagcggtaat aaatcggtct taaacgcttt ataaaacacg
     9841 tccatgcctg tttgcatcag taaattatcc ggtttacgtt tttgctcata gcgttgaagc
     9901 acctgctcac ttgcaaaatt tttgcctttt ttgaccgctt gttcgatcac ctcaagcaag
     9961 gttttaacat ctttaaagcc taaatttaca ccttgtccgg caagcggatt aatggtgtga
    10021 gccgcatcgc cgactaatac caccccgttt tgcacatagt gctgagcgtg ctggcgggtt
    10081 agcgggaaag cccctgaagc ctgcacctga atgttgccta attctgcgtt gagccggctt
    10141 gggaaatgtt gctcaatcgc aactcccagc ttttctgctg gaagcatttt cagttcggca
    10201 attttttgtg gcgaatcgta ccaaacaacg cagccgtgat tgccggaaag cggtaaaaag
    10261 gctcgagggc cggttggata gaattgttgc caagtgccgc ttggagcagg tttttcggtg
    10321 gtaacggtgg cgagtaaaca gtgttggcga tattgccaag cggttaaacc aatgcccgcc
    10381 cattggcgaa cctgtgaatt tgcaccgtct gcggcaatca gcagtggggc agcaaaggtg
    10441 ttgccatcaa ccgtcacttc ccaatactcg cccgctttat ttgcaaaaat ttgctcaaat
    10501 ccgaccgctt gtaggcagtt tggatattgc tttaattgct cccataacgc aatttgaata
    10561 acgttgtttt cgaccatatc gccgagcttt tccaaaccca aatcagctgc cgaaaaaaga
    10621 gtgttgaatc catcaatttc ccaggtttcg agctgggtat aaggggtgct tctcattgca
    10681 acaatttgct cccacacgcc caatttttct aacagttgaa tcgagcccat actaatggcg
    10741 gaaattcgca aatcgtagtc tgaatcagga ttaaaggtcg gcagagggtt tttctccaac
    10801 aaggctaaac gcagcccctg ttttgctaag cctagagctg ttgctgcccc aatcataccg
    10861 ccaccgatta caatcatatc taattcagtt ttcatttttt cttccttttt agttaggcaa
    10921 aatactgcta aaacgccttg aattgtagtg catttcagat taggatcaaa ccgatttatc
    10981 tatttatcta aggagcgttt tatgtcttgc aaaactcatg caaaccacga gcacgaacat
    11041 ggcgaaggct gtggtcatac tgctattaaa cataacgacc atatcgacta tttacacgat
    11101 gggcatttac accacaaaca cggcgatcac tatgacgaac acgttttaga agtgaatgaa
    11161 accaacccgg cagactgtaa tgccgcccat tgctgcggcg accacgttca tggtgagggt
    11221 tgcggacacg aagctgtgcc gcacggtgat catatcgact acatcgtgaa cgggcgtttg
    11281 caccaccaac acggcgacca ctgcgatgac cacgggccgg ttgaaattgt gaaataaggt
    11341 ttttgatggg aggttttagc ctcccattat tatttttgcg atctcgctcg aaaaattgaa
    11401 cattgctgct acccctcttc cgattttcct tttatgctct cggcattgat ttctaacacc
    11461 gaaatgtgat gaccttagcg attgtttaca gccgagcctc cattggcgtt gaagccccgt
    11521 tggtgacgat tgaagtgcat atcagcaacg gacaaccaaa attaaccatt gtcggcttac
    11581 ccgaagccac agtgaaagag gcaggtgatc gtgtgcggag tgcgttgttg aacgccaatt
    11641 ttatctatcc gccacaacgg atcacgatta accttgctcc ggcagattta cccaaagaag
    11701 gcggacgttt tgacttgccg attgcgattg gcattctggc agcctcaggg caaattgatt
    11761 ctgatttatt aacgcagttc gagtttttgg gcgagttggc actgacaggg catttgcgag
    11821 gagtgcacgg cacgattccg gcagtgattt ctgccgataa agccaaacgg cagatgatct
    11881 tagccaagca aaatgcgaat gaggcatcgc ttgtttccaa tgccaccacc tactttgccg
    11941 gctcgttgtt ggaggtggta aatatgctca acaaacggga taaactgccg atttgtcagc
    12001 acatttcgca acattctgcc gaaattcgcc cgcttgtcag ccgagatctt actgatatta
    12061 tcgggcaaca acacgccaag cgagccttaa tgattgccgc tgccgggcaa cataatttgc
    12121 tgtttctcgg accaccggga acgggtaaaa caatgttggc aagccggctg gcggatttac
    12181 tgccggaaat gaccgatgag gaggcgattg aaaccgcctc ggtgaccagc cttgtgcaaa
    12241 atgagctgaa ttttcagaac tggaaacaac gcccgtttcg ttcgccccat cacagtgcat
    12301 caatggtggc attagtggga ggtggctcga tccccaaacc gggggaaatt tcacttgccc
    12361 ataacggtgt gctgttctta gatgaattgc ctgaatttga acgtaaagtg cttgatgcgt
    12421 tacgtcagcc gttggaggca ggcgaaattg tgatttcccg agccaacgct aaagtacaat
    12481 ttccggcacg ctttcaatta attgcagcga tgaacccaag cccgacaggg cattatcaag
    12541 gcacgcataa ccgcacttcg ccccaacagg taatgcggta tttaaaccgc ctttccggac
    12601 ctttcttaga ccgtttcgat ctctctatag aagtgccact tttgccacaa ggagcattgc
    12661 aaagtgggac ggagaatcga ggagagacca ccgagcaggt tcgcaagcgg gtgtttttag
    12721 ccagaaaaat gcaatttgag cgagcaggta aaatcaattc tcaactcacc acccgagaaa
    12781 ttgagcgaga ttgccaatta tcggcacaag atgccctatt tttagaaact gcattgacaa
    12841 agctcggttt atcggtacga gcctaccacc gtattttgaa agtttctcgg actattgctg
    12901 atttagccgg cgaggcgaat attcaacaac tgcatttagc ggaagcactt ggttatcggg
    12961 cgatggatcg cttattgcaa cggttgcagg gggagcccta acacctagca cggctgagcc
    13021 gtactaggtt atatttagcc tacggctgat taagccccac tggggctttt gtgcagcaga
    13081 gctgcaccgt tatgcttaac cgattgcgac cgtaggtcgc tgtcggagtg gagtatcaat
    13141 aaccccaatt tctccacaaa atttttagca ttagcccgat gagcgttatg ccctgcatta
    13201 gcaatcagtt gtgttggtag ttggtggctt gatgccattt tgcgaaattt ctgatccttt
    13261 tcaccgataa aaaagacaat attcttatcc gtctgcttga gtttttcggt gtagtcaggc
    13321 tgtttggcga ggctggtggc ttccagcatt tgagcaattt gctctccttg attgtgcttt
    13381 ctgctatcaa tataacattc tctttccgta tcgcttaaat ccgcaaaaac aggctgtttg
    13441 taccaatctt caagcacttg ttcgatgggt tcttgacgga aacggtttgc ccaatggcgg
    13501 tcattttgcc ggcgaatctg gcggtcgttt tctgtgctta agccgatatt cgccccttct
    13561 aacaccacgc cctgcagatt cggattatcc atatttagca cataatcgag tgccgctcgt
    13621 ccaccgagtg agtagccgac taaatagaaa ggtcgtgagc cgattaggct ttcaagggtg
    13681 gcgtgtagtt gttgtcgcag gtcggcaaaa tcggcacagg taatgtgctt actatttccg
    13741 tgaaaaggaa ggtcaatggt aagcggtcga atttgcggaa aattttgcaa atgttggaga
    13801 acagcttgcc agtcttgttg tgagccgagt agcccgtgta agaaaataac aggtgtgcct
    13861 tgttgagcgt gccattgata ggaaagcata gtcgatacat ttttacccct cgcaggattg
    13921 gcgaggggca gttgtgatta ggcaatttcc gcctgtgaaa tttgttttag caatgattta
    13981 tacatattgc tgccgtcgtg ttcatttact ttgatttcca caatggtaac acctttacga
    14041 gcataggcgt gtttgagttt ggtttttaaa tcagcccaag taaacggacg gatatattcc
    14101 agcccaaaca ttgtcgccac ttgtgcaaat tcaaagttat gtgccaagcg gtaatattga
    14161 tctttcgctt tgctttcaac cggtaacata tcaaaaattg ccccaccgct gttattcacc
    14221 acaaataaaa tggtcggctg gttaattttg gtgagcaatg caattgagtt aagatcgtgt
    14281 aatgcggaaa tatcacctac cacaccgaca gtcgggcggt cgctaccttt tgcaatgcct
    14341 gccattgtgg caattaagcc atcaatgcca cttgccccac gattagtgta aaccgggtag
    14401 ccttcaggca atttgcataa ggcatcaact aaacgcacaa ataaactatt gccgatgaat
    14461 agattaccat taattgctaa aatatcttga atattatgtg caagtgatgc ctcacttaaa
    14521 cttccaccca cttgctgctc aataaaggtc gcacagaatt gtgaaagtgc taagggttca
    14581 agcaaccacg gcttttggcg aagtggtggg tgaacacgca agaagtggtg tgctttagca
    14641 acaaaacgag tttgatgatg tgcgttcggg ttaatgtaat cttgcgactc atcaaccagc
    14701 caaaattcgc cttgataggc ttttaagaac tcattcactc gtttgctgac aatttgtgtg
    14761 ccgaattgaa tgatgatatc ggcttgcaat aaacgttgtt tcacggtttc gttagagagc
    14821 caaatatccg catacggtag gcttgcttca atgcaagatt gcacatcgct taataagcac
    14881 cagcccattg tttccgccca gttctttaag cctgtgcctt gctctggcgg taatttgcct
    14941 accacaataa caccacgttt ggttcgccag taatcccaat tttcgtgcat cgagacttca
    15001 ctttgaattg cctgttggtc aatccatttg gtttgggaat tattcaacca accttgaatt
    15061 gggcgtaacc aaggggtatt tagaatttcg ttaatgtcag cttcataaag cggctcttca
    15121 aaaggggcat taatgtgcaa cacgcctcct tgttgagctt gagtagtgca agcctgttca
    15181 atggttgaaa ctaaccagct tgcattataa gcctcgtttg cttttggtaa gtttatgctc
    15241 gcagcaggat aatttgcaaa aatgccttgt tgagtaatgg cttggtttgc accacaacca
    15301 attaattctg ttgggcgatc tgcagaaaga ataatcaggt tttgatggct aaggcttgcc
    15361 tcaattaccg cagggtaaag attagcaacc gctgtgccgg aggtaacaat caccgcaaca
    15421 gggctattag ttgctttggc aatgcctaac gcataaaagc ctaaaccacg ctcatcaaag
    15481 tgggtgtggc attctgcata ttgggtttgc tgaagttgta ctgcctcaag ggttaatggg
    15541 gtggaacgag aacccggtgc gatacaaaaa tgctttacgc cataacgcat taacgcactt
    15601 aaaatcactt ttgcccaagt gcgattaaaa gaacttacac tcatattctt ctctactcat
    15661 taataattcg ataaatagcc caaattgtag caaaaagtta aattattttg gtaagttttt
    15721 agcaaaatag gtgattttta ataaaattac cgcccaatgc tgatgagtag cattgggcgg
    15781 taatcataga tttgtaaaaa atttacaaaa aataaccgct tgtattaatt aattccaatc
    15841 caattcggta acgccatcga gatagcagga atgtaggtga ttaacaccaa gaagcctaat
    15901 agcagtaata gccacggcat tgcggcttta attacatcag acagtggcat tttcgatacc
    15961 gcagaggcaa cgaataagtt caagccgatt ggtggggtga tcaagccgat ttccatattc
    16021 accaccataa taatccctaa gtggattggg tcaatgccga gttgtaccgc aatcgggaat
    16081 aaaattgggg caaggattaa gataatcgca gacggctcca taaatgcccc cgcaattaat
    16141 agcacgatgt ttaccactaa taggaatgtc cacggctgta aattccattc aaccactgta
    16201 ttagtgatga gctgtggaat ttgttcggtc gtcagaacgt gggcgaataa cattgcattg
    16261 gcaatgataa acatcaacat tacgcttaat ttggcggatt ccagcaagac tttcgggcaa
    16321 tcacgcagtt tgacatcttt atagataaag acagccacaa accacgagta aaccgctgct
    16381 accgctgcag cttcggttgg ggtgaagatg ccgctgtaaa taccgcctaa gataatcacc
    16441 atcagcaata agccccaaat cgctttacgg gcggcggtaa accactcttt taccgttgca
    16501 cgaggctgtg ccggtaagtt tttaatacga gcgacaacgt agatagcaat catcaatacc
    16561 aagccaagca ttaagcccgg cacaacccct gccataaata atttaccgac tgaggtttca
    16621 gttgaagcgg catataccac cattactacc gatggcggaa ttaagatacc aagtgtacct
    16681 gcattacaga taatccccgt accaaaaccg accggatagc ctgaacgcac cattcctgca
    16741 atggcaatcg aacctactgc tgctaccgtt gcggggcttg agcctgaaag agcagcgaat
    16801 agcatacaag acattaccgc cgcaatagct aaaccaccac gaatatgacc gacggttgca
    16861 ttggcgaaat caattaagcg ttttgctaca ccgccagttg tcataaacgc acctgcaagc
    16921 aagaagaatg ggattgcaag gagcgtatag tgttctgagg tttcaaatag tttgatggct
    16981 aaggagctga cagaatcttg gctgaaaaac ataatagtaa cagcaccagc taaaccgagt
    17041 gaaatcgcaa tcggcacgcc aagaaacatt aagataaata acaagctaaa tagcactaaa
    17101 attatcatta ttcttctcct tgtgcgagtt tcattgcgtc ttcactttca tctgctaagc
    17161 ctaagcccac ttgtttgccc tgtaaaatac gcactaaaat ttcaataaaa cggataccga
    17221 ttaaggcaaa accgacaggc ataattaaac caacgtgcca ctgtttgagg tggaattgat
    17281 gtaaatcttc tgctccaata tctgccacat acatcgctga aatccactca tagctggcag
    17341 aggcaattaa gaaggcatag gctaagcagg cggcaatcgc aatcaatcct acgacttttt
    17401 gggcgttgcg tgagagtttt ttcaccaata catcgactcc gatatgccct gcggtacgca
    17461 ccccataaga cgcaccaaag aagataagcc aaccaaagca gactttggtt aaagccacgc
    17521 tccaagtcat ttcttgagcc atttccataa tccattcgcc taacggctca gcaaaactcg
    17581 cggtaaacgg cacagcttcg gctaaatcaa agaagacatt ataaaggttg ttaaacatca
    17641 cataagtgaa ggtaacaagc gtcattacca ttagcaaaat ggcgataaaa cactcctcaa
    17701 aacgctccca gaaccatttt aaagaagaca tttggcactc cataaaaatg tggtatcgat
    17761 gttttctcgt aggggcgggt cttgtgcccg ccccttgtat atctcaaatg agttaaacta
    17821 agcaacacaa cccgttatcg gcatctcgtc tcgtcccttg aaagcacttc gttgtgtagc
    17881 ttagagagcc gataactttc atcacaaaaa ccgaacagtt gcacgaacac aacccgaata
    17941 caaggcaggt tacgatgaat gaacttaatt tctgtggcat tgatgttgct aaacgtagtt
    18001 ttgttgtcgg tatcagcagt cgaaaacaga ccaaaacttt taccaacaac cccaaaggta
    18061 tccaacaagc ggtggaatac ctgaaaaatt gtgcaaatct aagcctagtt gtcttagaaa
    18121 gcacaggcgg cttggaaatc ccactcgcca aagcacttca tcgtgcggat tttcgggtgg
    18181 tgattgctaa tccacgccaa acacatcagt ttgcaatggc taaatcgttg gcgaaaaccg
    18241 atgcaaaaga tgcaaaaatg ctggcatctt acgccaaaat gctggttttg gacgatgagg
    18301 ttgaaaaaca actttatctt ccgccaacac ctgaaatgga gcaattagaa gcattagttt
    18361 tacgccgtac ccaactggtt gaaatgcggg tggcggagaa aaaccgcctt catcaagtcc
    18421 acgacagcca gcgggaaagt gtccaacagt tgattgccca ttttgaccgt ttaattgatg
    18481 agctggaaac acaaatcaat caagcgactg ctgtatttga cgacacttta aaaaagttca
    18541 atgacatcaa agggataggt gcaatcacga gtgcaacttt gatgtgaatg ctccctgaac
    18601 tcggcaagct caaccacaag caaatcgctt cactggttgg tgttgcccca ctcccgaaag
    18661 agagcggtag ccagaaatgg catagtcgct gttcgggtgg gcgacaagcg gtacgaaacg
    18721 tactttataa tgcggcgaat gtcgcccgcc aacacgaacc gaagttcaag gcattttatg
    18781 aacggctgat tgctcgtggc aaaccgttca aagtggcggt aaatgcctgt atgcgtaagc
    18841 ttttaaccgt gattaatgcc atcgtccgtg atggctcaca gtggaatgct cacgcggttt
    18901 aattaacgaa tttgtatctt gccaaaaatg ttggggagat acagttgcta cacatcattt
    18961 cacattatca ataaaaataa gaatgcgacg agcctaaaaa gccaccgcat tcgctcaaaa
    19021 gaacaataga taaagagact actgtttatt tgccgcttcg gccgctttga ttaaatctgc
    19081 accgatagca tcttcaaact gcttccatac agggcgcatt gcttcacgcc atttttcgcg
    19141 ttgctcatca gttaaagtga tgatttgcga agcacctgaa tcgacgattt tttgcttatc
    19201 gctttggttg agatcgtgag attgtttgtt cacttccaca gacacctcat ctaagatttt
    19261 ggttagttct gtacgcacat cttcaggaat tttgttccag aaatcggtgt tcacgataac
    19321 catataagat aataagccgt gattagatgc agtaatgaat ttttgtactt cgtgaatttt
    19381 ttgtgagtag atgtttgaat acgggttttc cgcaccgttt acaacgcctg tttgtaagcc
    19441 ttgatacatc tcgccgaatg ccattttacg cggtacagct tttaatgctt tgaactgtgc
    19501 atctagcact tctgaagcct gtacacggaa ttttaaacca cgagcatctt tcggctcacg
    19561 taactctttg ttcgcagaaa gttgttttaa atcattgttc cagtaagata aacctgtaat
    19621 acctttgtct tccattgatt ttaacaatga ttgacctgct tcagattgct caaaacgttc
    19681 aaccgctttg atatcgtcaa acaagaacgg tagatcgaaa atttgaattt ttggtgtgta
    19741 tttatcaaat ttagccagtg atggagcaag catttgtacg ttatttaaca ataatgcttc
    19801 catctcttta ccgtctgcat aaagtgaaga gtttggataa acttccactt tcactttgcc
    19861 gtttaagcgt tcgtccacta atttttggaa cattaacgca ccctgacctt tcggggtctc
    19921 gtttgctaca acgtgtgaga atttgatgac gattgggtca ttagcaaaag cagataccga
    19981 gaatgcagca gttacagcta atgccgcagc caattttttg aatggt
//
LOCUS       DANJWC010000026        19255 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.026, whole genome shotgun sequence.
ACCESSION   DANJWC010000026 DANJWC010000000
VERSION     DANJWC010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19255
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.026"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..9464)
                     /locus_tag="PWX02_000710"
     CDS             complement(<1..9464)
                     /locus_tag="PWX02_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HDL1728745.1"
                     /translation="MNKHCFRTIFSKTLQRIVVVSELAKSAGKSESESMTSGAILQKI
                     CKIRPLAFSLFCALGFITLSDNAMAETLIIQADSTAPKNQQPIILQTANGLPQINIQT
                     PNDKGLSHNKYSQFDVAEKGAILNNSRTNIATQQAGMVQGNPYLARGEAKVILNEVNS
                     SKPSVMKGYVEVAGKKAEVIIANPSGLHCDGCGIINSDRATFTTGKPQINNGNLDSFV
                     VEKGKVTVAGKGLDNSRVDYTDILAREAKINAGVWSKKETKVITGKNTVKRSNSDNNL
                     QIIHTNQPLAGEEKPQVAIDVGELGGMYAGKIHLIGTEQGVGVRNAGHIGASADTLTI
                     DSQGRIVNSGTFNAQRQVQLNAQHGIDNQGKIENKQGNIQLNSKADIQNSGSIVARGG
                     NIQKKAETKIQQSGETVAKGNITYTARQIHADKNSLIAAGVDVTDTAQGEVRKLEAQS
                     VAGKTIQLTAPEKTTVQGKNIASGKINVAAAEIDANQSQNNAHTVEYSAKSGDIQANS
                     AQFSANQIKLTTPKWLSTEGSQLTAEVIEAQQANLNARGSVWKQTGEQDFRLKAESIH
                     TDSARFSVGGHFEIDGNRLTNNQGLLSSGKRLGLNLNDAVDSVDGKLVAKGELSIRAP
                     LLNNQQGLILSGEKQAITVNTLKNQSGVITSQTEQTLDITSELQNQGGRISASGVAIN
                     AQSLDNREQGEISSVKELALTVSQQVDNRHGVIQSVGNLTLNSTLLDNQGGVLKSATD
                     ITLNVPTVLNNRISENGSLIEAGGSLTMNSFEVENQGTLARQPQPIQGIIASQFVLNS
                     TKQLNNEKGGIYIGSSARFTLSDSLNNRNGEILSWGDLAIFGGKSLTMTNTEGKVSAN
                     NGLEITAKALSGDGDVQAGDIRLNLQENFDITKDINANRSMIIQTEGDILNRKKLAAN
                     DRLQLTANQITNAQNARISSADTRLTATSTVYNEGLINSRSDNDNAQTVIKAQEIHNI
                     GTGNIYGDKVALGAERILNQDKDGTSATIAARQSLGLAAKVIENNTLVYEANKKSGSL
                     IYSGGEIAIGRQLNEQDTVIGNAEQLHNRSSIIEAEGRIQLNADKVANVNSHYRSELE
                     TTDRQTVNEHFILPRDKHNRGGSDVYDNGHLPTINVNQLRKGYYRKTWSYWQKYDEAN
                     LPVITDISQLNATTVLAKPYAQSCANDESVDCTLLPAGNYKKDNPVWTWFNLTPPAED
                     APEMTPEIIQAMNEEDLANQHLSEAEQEKLQKEQGQGIAPVVPARPIEPVRGSTETEA
                     QYQKRLQTYQAELKAYEAAEAAYQRYVLLKPLIDWEAKYGANIQALDEAIAKHNKANL
                     SQEYPHFWNLWIKERMVKENITKASLPAQILAGGDLTYQSDEFINDKSWVIAGKALNP
                     IGRSRIANLDDADAVRQDWELGRRQFSFTEYHGGTRRRHSRYDENDGPLARLKEVHKD
                     MNIFVELENTSPTAYTDYVDAKAANAVKPEGNQVERGNLPDAPNPPTMAIERIPSPNA
                     AAFEVRSMTVDTRLPNQGLYRINPQADSHVLIETDPDFTDKKRWLSSDYMFNALRHDH
                     NNVHKRLGDGFYEQRLIREQINRLTGRQFLGDYTDFDSQYKALMDAGITFAQKFNLRL
                     GITLSPEQVASLTSDIVWFETQTVRLPSGETVSVLVPKVYALARKGDIDGKGSLISAD
                     ALTINSKEIVNQGTIAGRRLAQFNAERLENSGTLSGGVLAAKVSGDLENIGGKIEADK
                     AILLDIAGNFNHTSTTRTTQIDLDGFKRAETNLDRKALLHVKDENGLLQVQAGNITSA
                     GADIINDGQGQTYLSAKNQLNLTALQVGFDEKMGGGNHYRNEALNDVVISRVQGKGDV
                     VVSGKNLYSEGADLDAKARLALLAENDMVLGSATRSRDYEEYHKTKSGGALAKKTKTT
                     YDNISETEHKGTELTGEDILVKAGHDIQGESLLAVSRSGDIDIIGGNNVTLSSATNQL
                     SEQHIRETKRSGFLNGGEIGFTIGSQKTRQENSLEGAIQSTARNTLGSEGGNIRIQAG
                     NKASVSNVDVLISNEKTAVISGKNGVRVESGKDVINSTDNYEFSQSGLSIALSTPVTD
                     AVQSARQSVQKAKATQNEKLKGVYAVKAAEDAVIAAQNAQKVAETLGNLGNALTENHA
                     AAENPAVKISVSLGTQKQQRESRSQSVTHSKSTLNGGNIALISGAGKVALEGVDTRVK
                     DTLLLDGKLGIESKGVADTYQNNTKNKNHSASVGVFIGLDRDSFGIGFEAGVSGGKGK
                     ENIETETWQNNQLQAGKIVTNSANGKLMLDATNVKANHWEGEVQDFEARSRQDISKYK
                     SEYTEGSVHGTLAVGTTGVNGHVAYNGAKLNTAQVENQTAIDIGKGGMDVKVKKNAHF
                     EGAVMTSQAEKENNRFQAGTLTTGDIENHSELKTRSVAISGGTSGVNPMSALSLLGNK
                     NESERSTTKAAIGENIAITLTEDLNAETTLNHLNRDTQNANQKVTKHDISEVKETQEL
                     VKGIGEIAEKAYQIYTHSEREKVTEAKLELGKAQAQKASKEEIGKLEANLDRLQKEFD
                     KNYGTGSKTKRAVEAVTAALQGLATKDVGQAAVGLASPYLNAEIKKYTEGDTQANLIA
                     HALLGAVEAAAAGNNVLAGAAAGAGSEAAADFIVKTLYKGKSTQDLTEAEKQNVALLS
                     QLASGLASGLIGDSTQSAAVGADIGKRAVENNYLSNWQEQKRSQELKSCHGDARCENQ
                     VRLYWGLVDIGQDVSFVSGAVAGVPENLAESVEGLISMFSSPIETIVAMKELIAREDT
                     LSYIANMTKQEYLAKLDKMQVYYEQAGAVGSFNAGKEFGNMMTDVVTALTGAGALVKT
                     GVGTTAKIVTKSGKELAIPKTRSSASLGTCSFHGDMEVKTDKGYRQISSIKVGDKVLA
                     KNERTGITTYQKVQAHYSNPYDYTVYVEVADSKGKYHTIVSNKIHPFFTQVLSGRVPI
                     SSEGHSYNGEIAKAQWVDAQHLQKGYRLLAESGEWQTVTKVEIKQERLKAYNMTVEKD
                     HTYFIRGTNADNEGVWVHNDCWHALPNDAKQVKNIDGYKAYQFTDPKDGKVVTVVQKD
                     LNRFETPNHQAGVDPHFNRLSQQIDAETGRYLSTDNPVDGLYNRAYLRSETLQKIYAN
                     YEKLPNGNYRDLDGNIIKGPIDIGHMYGWEHRRLSLA"
     gene            complement(9517..11292)
                     /locus_tag="PWX02_000711"
     CDS             complement(9517..11292)
                     /locus_tag="PWX02_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HDL1728746.1"
                     /translation="MKTPQNLILSTVLLSASFVAEANSSSLVQQARIDATQQQIDAAQ
                     QHRQQQYQQNQAEKFQPKADVRLETQITDTLTLSNHELPCYPIHQITLIDYSPDNSLT
                     TSQFQWAFEKAAKSLHLTLPHCFGGEGLAVLMKQIQNEIIGKGYVTTRVVTAEQDLRG
                     GKLVLTVIPGKIRNTLVVDSGKVPRFTKLHALTGFTFAEGDILNVREIEQSLENLKRV
                     PTAEANIEILPSSSEKAEVGDSDLKISYSQAFPFRLNLGLDDAGSKSTGKLQTSATLS
                     IDNIFSANDLFYTSFTHSMKQKGDDNGRRASKNLTLYYSVPFGYWQLAFSHTHSRYHQ
                     EVFGAFDKSYLYAGESDTDKLTLSYLLYRDAKRKTTLSGSLWSRQSQNYIDGAEIGVN
                     KRRMAGWEAGFSHKEYFPTATLELSANFKRGTAARGALSAPEEDYGEGTSRPKIITAS
                     ASLTKPFFWGKQPWQWQSSLNAQWNKTPLVPQDRFSIGGRYTIRGFDGELTLSGERGW
                     NWRNELSWQFNPSHAFYWAVDGGRVSGWATENQLGHHLMGTAIGFRGGVKGFSYDLFA
                     GRPMRKPEGFKTSDTVAGFNIGYNF"
     gene            11448..12533
                     /gene="queA"
                     /locus_tag="PWX02_000712"
     CDS             11448..12533
                     /gene="queA"
                     /locus_tag="PWX02_000712"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDL1728747.1"
                     /translation="MLVSDFHFNLPDELIARYPTAERSASRLLHLNGETGEFADQQFV
                     DLYDHINEGDLLIFNNTRVIPARLYGRKPSGGKVEALVERVLDEHCCLAHVKASKAPK
                     EGAELIFGEDKLGEGKGFTATMVARHDTLFELVFNEDQPLFELLQQAGHMPLPPYIDR
                     PDEDADQERYQTVYSKVLGAVAAPTAGLHFDNAMLEKLKAKGVDTAFVTLHVGAGTFQ
                     PVRVETIEDHKMHAEYAEVSQEVVDKILATKAKGKRVIAIGTTSVRSVESAAQAAEKD
                     GKLIAPFFSDTAIFLYPGKTFKVIDALVTNFHLPESTLIMLVSAFAGYRNTMNAYAHA
                     VETKYRFFSYGDAMFINKNPNALSDVP"
     gene            complement(12560..13098)
                     /locus_tag="PWX02_000713"
                     /pseudo
     CDS             complement(12560..13098)
                     /locus_tag="PWX02_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13111..15402)
                     /locus_tag="PWX02_000714"
     CDS             complement(13111..15402)
                     /locus_tag="PWX02_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDL1728748.1"
                     /translation="MTLDRFCIALILFHLPILWLPKAFLFVGIVIGTLVLILSVYLRS
                     KLTAVLGLLVCIGYIQIIQVAKNAEKTTASKTPVQFEISKILKQTDYQTAIATLNTGE
                     KIYLNWQSEQPLLLNQFYQAELALRPISARSNIGNFDRQRWYFANHINMTATVRKVET
                     LAKTDYSLRTNWLNKVKAETDALPTQGFLLALAFGERAWLKTEHWEQFQQTATAHLIA
                     ISGLHIGLAMLFGVYFAKGVQWLCLKSELTALQAVGFSLYFARIVSFITAFGYSYLAG
                     FAGFAVPTARALLAIAFVLLCQVLRRHYTPWQYWGRIVALLILIDPLSLLSDSFWLSI
                     LAVASLILWYQYFPLSRFLNDEYRKKITKFNRLWLSLLHLQIGIWLIFSPVQLYFFEG
                     ISAFALLANLIIVPLYSFLLVPLILFTLLTDNLFSTWLLADYLAQFSLWLLDPFSNAW
                     FALSHWQQWQLLSLNLLVLVLLYCKLYRLSYKKWLSAVAVSLLFNLSFYLPKLFNQTQ
                     TEWIMFDVGQGLAMAFIYQQNKAVIYDTGSSWQANDGSRNSMAKMEILPYLKRNGIEV
                     EAIFLSHDDNDHSGGVVELLQAYPKARLISSSKVTYHNINPEPCIAGTSWQFGEWQLL
                     AIYPSHIAERAKNQDSCVILAKNDRLKILLTGDSGVEQERQFAHQLGKIDFLQVGHHG
                     SKSSTGETLLSITQPAFAVISAGRWNPWKLPNKQIIEKLNRRHINVLNTVETGMIRVK
                     FYPSSIEIEQARGAFSPWHKQFY"
     gene            complement(15544..16758)
                     /gene="sstT"
                     /locus_tag="PWX02_000715"
     CDS             complement(15544..16758)
                     /gene="sstT"
                     /locus_tag="PWX02_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDL1728749.1"
                     /translation="MSQSLSSKILGGNLVLRIAIGLVLGVLLAFINKEWATSIGVLGQ
                     FFVKSLRAIAPILVFVLVLAAIANKEVGSDSKLKPILVLYVLGTFFAALTAVVLSFML
                     PTTLELTASPDGLTPPQGIGEILKTVIFNLVDNPLVALSNANFIGILAWSIGLGIAFR
                     HGSASSKAFLNDLANAVSFVVKVVIAFAPIGVFGLVAETVAINGIDAFEDYARLLVVL
                     VGAMLFVALVLNPLLVYWKIRRNPYPLTFTCLRESGVTAFFTRSSAANIPVNMNLAKR
                     LGVRDEIASVAIPLGASINMAGAAITVTVLTLAAAYTQGIQPDFATALLLSVVASICA
                     CGASGVAGGSLLLIPLACSLFNIPNDIAAQVIGVGFVIGVIQDSVETALNSSTDVLFT
                     AAVSYAEDQKSA"
     gene            17013..17375
                     /locus_tag="PWX02_000716"
     CDS             17013..17375
                     /locus_tag="PWX02_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL1728750.1"
                     /translation="MKPTNKADFQHVINAAGYSMKGLKAAYINEAAFRQEIWCATILF
                     PLGLILGETNIEKALLVGTVLLVLVTELLNSAVESVVDRIGADFHELSGRAKDIGSAA
                     VFMAMVLLAITWILILFC"
     gene            17412..19055
                     /locus_tag="PWX02_000717"
     CDS             17412..19055
                     /locus_tag="PWX02_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HDL1728751.1"
                     /translation="MRLKKTLLSVALLSATSAMAYETGKTYRFTVLHTNDIHGHFWQS
                     DKGEYGLAAQKTLVDNIRKEVAEKGGSVLMLNAGDANTGVPESDMQNARPDIEGMNAI
                     GYDAMVLGNHEFDYPLQILSMQEKWAKFPFISANVINKKTDKPIVKPYVMLERGGLKI
                     AVIGLTTEDTAKLGNPDVTENAFFKNPIETAKNTLAEINQKEKPDVRIALTHMGYYFD
                     GKHGTNAPGDVTMARTLEKGAFDLIIGGHTHDTVCIDEKGQFKAKYTPGEECKPDFQN
                     GTWIVQAGEWGKFVGRADFEFKDGKTTLVKYELIPVNLKKTIKKEDGTKEYRLYQPEI
                     KADEATYTLLKKYQDEGDKKLNIEVGRVKGLLDGKREHIRFQQTNLGRLIAQSQMERV
                     KADIGIMNSGGIRTSLEEGVITYKHILTVQPFGNMIATVDLKGQELLDTLTTIALKEV
                     DTGAYPQFAGISMVVDRTAKTISDVKIGGKPFDKEKTYKVSLPDYVAAGGDGYPLLKK
                     HPSYVNTGFIDAEMLKKFFEENKIIEADKYDPKNQIIFK"
BASE COUNT         5135 a         4411 c         3841 g         5868 t
ORIGIN      
        1 gctaatgata atcggcgatg ttcccagcca tacatatgac ctatatctat aggacctttg
       61 attatatttc catctaaatc tcgataatta ccatttggca atttttcata attagcataa
      121 attttctgta acgtttccga gcgtaaataa gctcggttat atagcccatc taccggatta
      181 tctgtcgata aatagcgtcc cgtttcggca tcaatttgct gagacaaacg gttaaaatgt
      241 ggatctaccc cggcttgatg gtttggcgtt tcaaaacgat tgagatcttt ttgaactact
      301 gtcaccactt tcccgtcttt cgggtctgtg aactgataag ctttatagcc atcaatattt
      361 tttacctgtt tggcatcatt cggtaaagcg tgccaacaat cattatgcac ccatacccct
      421 tcattatcgg catttgtacc tctaataaag taagtatgat ctttctctac cgtcatattg
      481 taggctttta atctttcctg tttgatttct accttagtga cagtctgcca ctcaccactt
      541 tcagcaagta agcgatagcc tttttgcaaa tgttgagcat caacccactg agcttttgct
      601 atctctccgt tgtaagaatg cccctccgaa gaaatcggca ctctgcccga taatacttgc
      661 gtaaagaacg ggtggatttt gtttgacaca atggtgtggt attttccttt gctgtctgcg
      721 acttctacat acaccgtata atcgtatgga ttactgtaat gagcttggac tttctgatag
      781 gtggttatac cggtgcgttc atttttcgcc agcaccttat cgcccacttt aatgctggaa
      841 atctgtcgat aacctttatc agttttgact tccatatcac cgtggaatga gcaagtgcct
      901 aaactagcgc tcgatctggt cttaggaatc gcaagttctt tacctgattt ggtgactatt
      961 ttcgccgttg tccctacacc tgttttaaca agagcaccag caccggttaa tgctgtcacc
     1021 acatcagtca tcatattgcc aaactctttt ccggcattaa aagaacctac agcccctgcc
     1081 tgttcatagt aaacctgcat tttatcgagt ttagctaaat actcctgttt agtcatatta
     1141 gcaatatagg agagcgtatc ttctcttgcg atcaattctt tcatcgccac aatagtttct
     1201 attgggcttg agaacatgga gatcaatcct tcaacgctct cagcgagatt ctcaggaaca
     1261 ccagcaacgg cgcctgatac aaaactaaca tcttgcccaa tatcaactaa tccccaatat
     1321 aatcgaactt ggttttcaca gcgggcatca ccatggcagg attttaattc ttgtgagcgt
     1381 ttctgctcct gccagttcga gaggtagtta ttctccactg cccttttccc aatatccgcc
     1441 ccaaccgctg ccgattgtgt actatcccct atcaagcccg aagccagtcc gcttgcaagc
     1501 tggcttagca acgccacatt ctgtttctcg gcttcggtta agtcttgcgt ggatttgccc
     1561 ttgtagaggg ttttaactat aaagtccgct gccgcttcgc tccctgcgcc tgccgcagcc
     1621 cctgccagta cattgttccc tgccgcagcc gcttccaccg cacctagcaa tgcgtgagca
     1681 atcaagttag cttgggtatc gccttcggtg tactttttga tttctgcatt taagtatggt
     1741 gaggctaaac ctactgccgc ttgtcccacg tctttcgtcg ctaaaccttg caaggctgcc
     1801 gtcaccgctt ccaccgctcg tttggtttta ctgcccgtgc cgtagttttt atcaaactct
     1861 ttttgcaagc ggtctaaatt ggcttctaat ttaccaatct cttccttgct cgctttttga
     1921 gcttgtgctt tgcccaattc cagcttcgct tccgtcactt tttcacgctc actatgcgtg
     1981 taaatctgat aagccttttc cgcaatctcg cctatgcctt tcaccagctc ctgtgtttct
     2041 ttcacctcac tgatatcgtg tttcgtcact ttttgattgg cgttttgcgt atctcggttg
     2101 agatgattaa gcgtagtttc cgcatttagg tcttcggtga gggtgatagc gatattttct
     2161 ccaatcgctg cctttgttgt gcttcgctcg ctttcgtttt tattgcccag taagctcagt
     2221 gccgacatcg ggttcacccc acttgtccct ccgctaatcg cgacacttcg ggttttcaat
     2281 tcactgtggt tttcaatatc gccggtggtc agcgtgccgg cttggaagcg gttgttttct
     2341 ttctccgctt ggctagtcat taccgctccc tcaaaatggg cgttcttttt cactttaacg
     2401 tccattccac ccttgcctat gtcgattgca gtttggtttt ccacctgagc agtgttcaac
     2461 ttcgctccat tatatgccac atgaccattt actcctgttg tccctaccgc caatgtgcca
     2521 tgtacgctgc cttctgtgta ttcgctttta tatttgctta tatcttgtcg acttcttgcc
     2581 tcaaaatctt gtacctcacc ttcccaatga tttgctttga cattggttgc atccagcatg
     2641 agtttaccat ttgcagaatt tgtaacaatt ttacccgctt gcagttggtt gttctgccac
     2701 gtttcagttt cgatattctc cttgccttta cctccactca ctcctgcctc aaaaccaata
     2761 ccaaaactat ctctatctaa accgataaat acgcccacac tcgcactgtg attcttattc
     2821 ttagtgttgt tttgataagt atccgcaaca cctttggatt caatacccag cttaccgtca
     2881 agcagcaggg tgtctttcac tcgggtatcc accccttcca gcgcaacttt gcccgctccc
     2941 gaaatcaagg caatattgcc gccgttcagg gtgcttttgc tgtgggtcac gctttgacta
     3001 cggctttctc tttgctgttt ttgcgtgccg agactgactg aaatcttcac cgccggattt
     3061 tctgccgcag catggttttc tgtgagtgca ttgccaagat tgcccaacgt ttctgccact
     3121 ttctgagcat tttgtgctgc aatcacggca tcctccgccg cttttaccgc atatacccct
     3181 ttgagtttct cgttttgtgt tgccttcgct ttttgcaccg attgccgtgc cgattgcacc
     3241 gcatccgtta ccggtgtact caacgcaatg cttaagcccg actggctaaa ttcataatta
     3301 tcggtgctgt taataacatc tttccctgat tcaacccgta ccccgttttt gcctgaaatc
     3361 acggcggttt tttcgttgga aatcagcaca tcgacattgc tcacgctcgc tttattgccg
     3421 gcttgaatcc gaatattgcc cccttcactg cccaatgtat tgcgtgccgt gctttggatt
     3481 gccccctcta agctgttctc ttggcgtgtt ttttggctac cgatagtaaa gccgatttca
     3541 ccaccgttca agaaaccgct gcgttttgtc tcacgaatat gctgttctga aagttggttt
     3601 gttgccgaac tgagcgttac attattaccg ccgataatat caatatcgcc actgcgtgag
     3661 acggcaagaa gactttcacc ttgaatatcg tgaccggctt tcactaaaat gtcttcaccg
     3721 gttagttcag tgcctttgtg ttcggtttca gaaatattgt cataagtcgt ttttgttttc
     3781 ttcgctagcg caccaccact ttttgttttg tgatactctt cataatcgcg agaacgtgtt
     3841 gcgctaccca acaccatatc attttcagcc agtaacgcta aacgcgcttt ggcatccaaa
     3901 tccgctcctt cactgtaaag gtttttccct gaaaccacca catcgccttt gccttgcacc
     3961 cgtgaaatga ccacatcatt caaggcttca ttacgatagt gattgccacc gcccattttt
     4021 tcatcaaaac caacctgtaa agcggtcaaa ttcaactgat tttttgcaga taagtaggtc
     4081 tgcccctgtc cgtcattgat gatgtctgcg cctgctgagg tgatgttgcc tgcctgtact
     4141 tgcagcagtc cattttcatc tttaacgtgc agtagggctt tgcggtcaag gttggtttca
     4201 gcgcgtttga aaccgtctag gtcaatctgt gtggttcgag tggtggaggt atggttaaaa
     4261 ttgcccgcaa tatccagcaa aatcgcttta tccgcttcaa tcttgccgcc gatattctct
     4321 agatcaccgc tcactttggc agcaagcacg ccaccactga gtgtgccact attctctaac
     4381 cgttcggcat taaactgtgc taaacggcgg cctgctatcg tgccttggtt tacaatctct
     4441 ttactgttga tggtaagcgc atcggctgaa atcaggctgc ctttgccgtc aatatcacct
     4501 ttgcgtgcaa gggcatacac tttcggcact aataccgata ccgtttcacc gcttggcaag
     4561 cgaaccgttt gggtttcaaa ccaaacaata tcgctggtca agcttgccac ttgctccggc
     4621 gataaggtaa tgcctaaccg aagattgaat ttctgcgcaa aggtgatgcc ggcatccatc
     4681 agggctttat attggctatc aaaatccgta tagtcgccca aaaattgtct gccggttaag
     4741 cggttgattt gctctcgaat taagcgctgt tcataaaaac catcgcccaa acgtttatgc
     4801 acattgttat ggtcgtggcg tagggcattg aacatataat cactgcttaa ccagcgtttt
     4861 ttatcggtga aatccggatc ggtttcgatc agaacgtggc tatccgcttg agggttaatg
     4921 cgataaagcc cttggtttgg caaacgggta tcgaccgtca ttgagcgaac ctcaaatgcg
     4981 gctgcattcg ggcttggtat gcgttcaatc gccattgttg gcggattagg ggcatcgggt
     5041 agattacccc gttcaacctg attgccttcc ggcttcacgg catttgctgc tttagcgtcc
     5101 acataatctg tataagcggt cggtgaagta ttttccagct cgacaaaaat attcatatcc
     5161 ttatggactt cttttaaacg agcgagagga ccatcatttt catcgtagcg ggagtggcga
     5221 cgtcttgtgc ccccgtggta ctcagtaaaa gagaattgtc gccttcccag ttcccaatct
     5281 tggcgaacag catctgcatc atcaaggttg gcaattctac tgcgaccaat cgggtttaac
     5341 gctttgcctg caataaccca acttttatcg ttgataaatt catcgctttg ataagtgaga
     5401 tcgccacctg ccaagatctg tgccggtaag ctggctttgg tgatattctc ttttaccata
     5461 cgctctttaa tccacaaatt ccagaaatgc gggtattctt ggcttaaatt cgctttattg
     5521 tgctttgcaa tcgcttcatc taatgcttga atattggcac catattttgc ttcccaatca
     5581 attaacggtt ttaatagcac ataacgctga taagccgctt ctgccgcttc ataggctttc
     5641 agttccgctt gatatgtttg tagtcgtttt tggtattggg cttccgtttc cgtagagcct
     5701 ctaacaggtt caatcggtct tgccggaacc accggtgcaa tgccttgccc ttgttccttc
     5761 tgtaattttt cctgctctgc ctcacttaaa tgctgattcg ctaaatcttc ttcattcatt
     5821 gcttgaataa tttcaggcgt catttccggt gcatcttcag ctggtggtgt taaattaaac
     5881 cacgtccata ctgggttatc ttttttataa ttacctgcag gtaatagagt acaatctaca
     5941 ctttcatcat tcgcacagct ttgagcatag ggttttgcca atacggtcgt ggcattcagc
     6001 tggctaatat cggtaattac cggcaagttt gcttcatcat acttttgcca atagctccac
     6061 gttttgcgat agtagccttt gcggagttga ttcacattaa ttgtcggcaa atgcccgtta
     6121 tcatatacat cacttccacc tcgattatgc ttatctcttg gcaaaataaa atgttcgttc
     6181 acagtttggc gatcagtggt ctccaattcc gaacgataat gactatttac attcgctacc
     6241 ttatccgcat tcaactgaat acgcccttca gcttcaataa tactgctgcg gttatgtagc
     6301 tgctctgcgt tgccaataac ggtatcttgc tcatttaatt gacgtccaat cgctatctct
     6361 cctccgctat atattaatga accgcttttc ttattcgctt cataaactag ggtattattt
     6421 tcaattactt tagccgctaa accgagagat tgacgagcag caatagtggc agaggtcccg
     6481 tctttatctt gatttagtat cctctccgca cctaacgcca ccttatcgcc ataaatattc
     6541 cctgtgccga tattgtggat ttcttgagct ttaatcacag tttgagcgtt atcgttgtcc
     6601 gagcggctat tgattaagcc ttcgttatag acggttgaag ttgcagtcaa acgggtatct
     6661 gccgagctta ttcgggcatt ttgtgcattg gttatttggt ttgccgtgag ctgcaagcgg
     6721 tcatttgcgg ctaatttttt gcgattgaga atatcgccct cagtctgaat aatcatcgac
     6781 ctattcgcat tgatatcttt ggtaatatca aagttctctt gcaaattcag gcgaatatcg
     6841 cctgcttgta catcaccatc accggataac gctttggcag tgatctccaa accattattt
     6901 gcagaaactt ttccctcggt attcgtcatt gtgagtgatt ttccgccgaa aatagccaga
     6961 tcgccccaac ttaaaatttc cccattacgg ttatttaagc tgtcgctgag ggtaaatcgt
     7021 gctgatgagc cgatataaat accgcctttt tcattattga gctgttttgt gctgttcagt
     7081 acaaactgcg atgcaataat cccctgtatt ggctgaggtt ggcgtgccaa ggtaccttga
     7141 ttttccacct cgaaactgtt catcgtaagt gagccacctg cttcaataag tgagccattt
     7201 tccgagatcc gattatttaa gacagtcggt acattgagcg tgatatctgt ggccgatttc
     7261 agtaccccac cttggttatc caaaagcgtg ctatttaggg tgagatttcc cacactttga
     7321 ataacaccgt gccgattatc tacttgttgg gaaaccgtga gcgctaactc ttttactgaa
     7381 ctaatttctc cctgctcacg gttatctaag gattgagcgt taatcgcaac accgctggcg
     7441 gaaattcgcc cgccttgatt ttgcagttcg ctagttatat caagggtttg ctccgtttga
     7501 gaggtgatca cccccgattg attttttaac gtattgaccg taatcgcctg tttttcgcca
     7561 ctcaaaatca gaccttgctg attattcagc aatggagcac gaatagataa ctcgcctttt
     7621 gcaaccaatt tgccatcgac tgaatccaca gcatcattta aatttagccc taaacgcttg
     7681 ccactactta atagcccttg gttatttgtt aaacgattac cgtcaatctc aaaatgtcca
     7741 cccactgaaa agcgagccga gtcagtatgg atactctctg cttttaggcg aaaatcctgc
     7801 tcaccagtct gcttccaaac cgagccacga gcatttaaat tcgcttgttg ggcctcaatc
     7861 acctctgcgg ttagctggct tccttcagtg gaaagccatt ttggggtggt gagcttgatc
     7921 tgatttgcac taaactgcgc actattcgcc tgaatatcgc ccgattttgc actgtattca
     7981 acggtatggg cattattttg gctctgattg gcatcaatct cagctgccgc cacgttaatt
     8041 ttccccgaag cgatattttt tccctgcaca gtggtttttt ctggggcagt gagttgaata
     8101 gttttccctg ccacagattg agcttccagc ttccgtactt caccttgtgc agtgtcggtt
     8161 acgtccaccc cagcggcaat cagtgagttt ttgtcggcgt gaatttggcg ggcggtgtag
     8221 gtgatattgc cttttgcgac cgtttcgcca ctttgctgga ttttagtttc ggcttttttc
     8281 tgaatattgc cgcccctcgc cacgatggaa ccgctatttt gaatatcggc tttcgagtta
     8341 agctgaatat tgccctgttt attttcaatt ttgccttggt tgtcaatccc gtgttgggca
     8401 ttgagttgca cttgtctttg agcgttgaac gtgccgctgt ttacaatccg cccttgactg
     8461 tcaatggtga gcgtatcggc acttgcaccg atatgcccag cgttacgcac gcccacgccc
     8521 tgttccgtac caatcaggtg gattttgccg gcatacattc cgcccagctc acccacatca
     8581 atggccacct gcggtttttc ttcccctgca agcggttgat ttgtatgaat aatttgcaaa
     8641 ttgttgtccg aatttgaccg cttgacggtg tttttgccgg taatgacttt agtctctttt
     8701 ttcgaccaga caccggcgtt gattttcgct tctcgggcga ggatatcggt gtaatccact
     8761 cggctgttgt ccagcccttt gccggcaacc gttaccttgc ctttttccac cacaaagcta
     8821 tccagattgc cgttgttaat ctgtggttta cctgtggtaa aagttgctcg gtctgaattg
     8881 ataatgccgc agccgtcaca gtgcaagccg ctcgggttgg cgataatcac ttccgctttt
     8941 ttgccagcca cttccacata acctttcatt accgaaggct tgctggaatt gacttcattt
     9001 aaaatcacct tcgcttcgcc acgggcaaga tacgggttgc cctgcaccat tccggcctgt
     9061 tgggtggcga tgttggtgcg gctgttgttg agaattgccc ccttctctgc tacgtcaaat
     9121 tggctatatt tgttgtgcga taatccttta tcgttcgggg tttgaatatt gatttgtggc
     9181 agcccgttgg cggtttgcag aataatcggc tgctgatttt tcggggcggt gctatcggct
     9241 tgaataatca aggtttccgc catagcgtta tctgataagg taatgaatcc caacgcacag
     9301 aacaagctga acgcaagcgg tcgaattttg caaatttttt gtaaaattgc accgcttgtc
     9361 atactttcac tttctgattt accggctgat tttgctaatt cagataccac cacgatacgt
     9421 tgtaaggtct tgctgaaaat agtgcgaaaa caatgcttat tcataataat tccttaatag
     9481 cacaatatag ttaaatataa tacgctcaat tatttattaa aaattgtagc caatattaaa
     9541 accggcaacg gtatctgagg ttttaaaccc ttccggtttt cgcatcggtc taccggcaaa
     9601 caggtcataa ctaaaccctt taactccacc ccgaaaccca atggctgttc ccattaaatg
     9661 atgtccgagc tgattttccg ttgcccaacc ggacactcga ccaccatcta ccgcccaata
     9721 aaaagcgtgg ctcggattaa attgccagct caactcatta cgccagttcc agccccgctc
     9781 accggaaaga gtcagttcac cgtcaaatcc acgtatggtg taacgcccgc caatcgaaaa
     9841 acggtcttgc ggaaccagag gggttttatt ccattgggca tttaggctac tttgccactg
     9901 ccatggctgc ttgccccaaa aaaacggttt cgtcaggctt gcacttgccg tgatgatttt
     9961 agggcgagaa gtcccttcgc cataatcttc ctcgggagca gataatgccc ctcgtgctgc
    10021 cgtacctcgt ttgaaattag ccgaaagctc aagcgtagcg gtcgggaaat attctttatg
    10081 cgaaaaaccc gcttcccagc ccgccattcg gcgtttgttg acaccgattt ctgctccatc
    10141 aatatagttt tgcgactggc gtgaccaaag tgaaccggac aaggtcgttt tgcgtttggc
    10201 atcacgataa agcaaataag agagcgttaa tttatcggta tcactttcac cggcgtagag
    10261 ataactctta tcaaaggcac caaatacttc ttggtgatag cgactgtgag tgtgtgaaaa
    10321 cgctaactgc caatagccaa acggcacgga atagtaaagc gtgagatttt tactggcacg
    10381 cctgccgtta tcatcgcctt tttgtttcat cgaatgggta aaagaggtat agaataggtc
    10441 atttgccgaa aaaatattat ctatagacag agtggcggag gtttgtaatt tacctgtgga
    10501 tttcgagccg gcatcgtcca gcccgagatt taagcgaaat ggaaacgctt gcgaatagct
    10561 gatttttaaa tcactgtctc ccacctccgc tttttcgctt gagctgggta aaatctcaat
    10621 atttgcctcc gccgtcggca cccgttttag gttttccaac gattgctcaa tctctctcac
    10681 attcagaata tcgccttcgg caaaagtaaa accggttaag gcgtgtagct tggtaaaacg
    10741 cggtactttg ccgctatcta ccactaaagt attgcgtatt ttgcccggaa tcacagttaa
    10801 aactaatttt ccaccacgta aatcttgctc cgcagtaacc actcgtgtag tgacataacc
    10861 tttgccgata atttcatttt ggatttgctt catcaacaca gctaaccctt cacccccaaa
    10921 acaatgcggt aatgtcaaat gaagtgattt tgctgctttt tcaaacgccc attggaattg
    10981 gctggtagtc agagaattgt caggagaata gtcaatgagg gtgatttgat gaatggggta
    11041 acaaggcaat tcgtgattgg aaagagtaag tgtatcagtg atttgtgtct cgagccgtac
    11101 atcagctttc ggctggaatt tttctgcttg attttgttgg tattgctgtt ggcgatgttg
    11161 ctgagcagca tcaatttgtt gttgagttgc atcaatgcgt gcttgttgta caaggcttga
    11221 agaatttgct tcagcaacaa aagaagcact taacaatacc gtgcttaaaa tcagattttg
    11281 tggggttttc ataagatcaa ataagtaaga cattcaacgt ttttagtcta taaaaattta
    11341 tgttgttttg caaactccgt tttatggagc tttatacata tctgacgact ttggtttttt
    11401 atgctattat ctccgctatt tttatactat agagatatag aataaccgtg ttagtttcag
    11461 attttcattt caacttaccc gatgagctga ttgctcgcta cccgactgcc gaacgctctg
    11521 ccagccgttt attacactta aatggcgaaa caggcgaatt tgccgatcaa caatttgtgg
    11581 atttatacga ccatatcaac gaaggtgatc tgttgatctt caataacact cgagtgatcc
    11641 cggctcgcct ttacggtcga aaaccgagtg gcggtaaagt ggaagcatta gtagaacgtg
    11701 tgttagacga acactgttgc ttggctcacg ttaaagcctc aaaagcaccg aaagaagggg
    11761 ctgaattgat ctttggggaa gataagctag gtgaaggaaa aggctttaca gcaacaatgg
    11821 tagctcgcca cgatacccta tttgaattgg tgtttaacga agatcaaccg ttattcgaat
    11881 tgctacagca agctggtcat atgccacttc caccttatat tgaccgcccc gatgaagatg
    11941 ctgaccaaga gcgttatcaa accgtttata gcaaagtgtt aggtgcagtt gccgccccta
    12001 ccgccggttt gcattttgac aatgcaatgc ttgaaaaact aaaagcaaaa ggggtggata
    12061 ctgccttcgt aacattgcac gttggtgccg gcactttcca gccggtacgg gtagaaacca
    12121 ttgaagatca taaaatgcac gccgaatatg ccgaagtgag ccaagaagta gtcgataaaa
    12181 ttctcgcaac gaaagccaaa ggaaaaagag tgattgcaat tggtacgaca tcagtacgct
    12241 cggttgaaag tgccgcccaa gcggctgaaa aagatgggaa attgattgct ccgttctttt
    12301 ctgacaccgc tattttcctt tacccgggaa aaacctttaa agtcattgat gcgttagtca
    12361 ccaatttcca tttaccggaa tccacgctaa ttatgttagt ctcagcattt gccggctata
    12421 gaaatacgat gaatgcctac gcccacgcag tagaaactaa ataccgtttt ttcagctatg
    12481 gcgatgcgat gtttatcaat aaaaacccaa atgcgttgag tgatgttcct taatttatga
    12541 gggttataag ggaataacat tatgaacaaa aacaatcttc caaccatttt aagggattgt
    12601 tttcaatata ttcaataatt tggaagtacg atgtttcatt acgaataatg tgttcataat
    12661 aattcctttg ccaaatttca gaaatatcgg tattttgacg aacccattta gttacgccaa
    12721 ttttaaaacc acgcacaata gagccgattg ttcgtgatgt tccccgtaag ggcgaaacat
    12781 ttttcgccct tatacatggg gcaaatgatg attttcccct acagtttcga cgatttcaat
    12841 aatgccatgt aaatgattag gcataatcac aaagctatgc aagcttacat ttggaaaatg
    12901 atcgggtata tcttcccaac attgacgtac aatattccct atcttattaa gcctcatttc
    12961 atcatcaaca atctcaccga gaaaatggtg tttattttta caacaaatag taatgaaata
    13021 gaatccttgt gatcggtaat cataatgctt tagacgaatt gaacgcctcc tatgaatctc
    13081 cggctgatat ttctgcattt ttcttattcc ttaataaaac tgcttatgcc aaggggaaaa
    13141 tgcccccctt gcctgctcaa tttcaataga acttgggtaa aattttactc ttatcattcc
    13201 cgtttctacg gtatttaata cattaatatg tcttctgttt aatttctcaa taatctgttt
    13261 attcggtaat ttccacgggt tccatcttcc agcagaaatc actgcaaatg ctggctgggt
    13321 aatcgataat aaagtttcgc cagtgctgct tttgctaccg tgatgtccaa cctgtagaaa
    13381 atcaattttg ccgagctgat gggcaaattg gcgttcttgt tccacgccag aatcccctgt
    13441 cagtaatatc ttcaagcggt cgtttttggc taaaataacg caagagtctt ggtttttagc
    13501 tcgttctgca atgtgtgacg ggtaaattgc caataattgc cattcgccaa attgccacga
    13561 tgttccggca atacaaggct cgggatttat attgtgatac gtcactttgc ttgaactaat
    13621 taaccttgct tttgggtatg cttgcaataa ctccactacc ccacctgaat gatcattatc
    13681 atcgtgactt agaaaaatcg cctctacctc aatgccattt cgctttaaat acggcaagat
    13741 ttccattttt gccattgaat tgcgactacc atcatttgct tgccaactgg agccggtatc
    13801 gtaaattacc gctttatttt gttgataaat gaacgccatt gccagccctt gcccaacatc
    13861 aaacataatc cattctgttt gggtttgatt aaagagctta gggagataaa aactcagatt
    13921 gaacaatagg gataccgcta ctgcacttaa ccacttttta taagatagcc gatataattt
    13981 gcaatacagc aatactaaaa ccaataaatt cagtgaaagt aactgccact gttgccaatg
    14041 gcttaatgca aaccacgcat tagaaaatgg atctaacagc cataggctaa attgagcaag
    14101 atagtccgcc aacagccacg ttgaaaacag attgtcggtt aagagcgtaa ataaaataag
    14161 cggtacaagc aaaaagctat acaacggcac aataatcagg ttggcaagca gtgcaaaagc
    14221 agagatgccc tcaaaaaagt agagttgaac cggtgagaaa atcagccaaa taccgatttg
    14281 taagtgcaaa agcgataacc acaagcggtt aaattttgtg atttttttac gatattcatc
    14341 gtttaaaaat cggctaagag gaaaatattg ataccataaa attaaacttg ctaccgctaa
    14401 aattgagagc caaaaactat cagaaagcag gctcaatggg tcaattaaaa tcagtaacgc
    14461 cactattctc ccccaatatt gccaaggggt atagtgtcgc cgcagaactt ggcaaagcaa
    14521 gacaaacgca atcgccagca acgctctggc ggtcggcacg gcaaaaccgg caaaaccggc
    14581 taaatagctg taaccaaatg cggtgatgaa gctaactatt cttgcaaaat aaagtgaaaa
    14641 tccgaccgct tgtaatgcgg taagctcgct tttcaagcaa agccattgca cgcctttggc
    14701 aaagtaaaca ccaaacaaca ttgccagccc gatatgcaag cctgaaatgg caattagatg
    14761 ggcagtagcg gtttgttgga attgctccca gtgttctgtt ttcagccacg ccctttcacc
    14821 aaaagccaga gccaataaaa agccttgcgt aggcaaagcg tccgtttcag ctttcacttt
    14881 attcagccaa tttgttcgca agctataatc agttttggct aatgtttcca ccttgcgaac
    14941 cgttgctgtc atattgatat gattggcaaa ataccaacgc tgtcggtcaa aattaccgat
    15001 attgctacga gctgaaatag gacgaagagc cagttcggct tgatagaatt gattaagtaa
    15061 caacggctgt tccgattgcc agttcaagta gatcttctcg cctgtattta gtgtcgcaat
    15121 cgctgtttga taatcggttt gttttaagat tttactgatt tcaaattgaa caggcgtttt
    15181 actagcagtc gttttttccg cattttttgc aacttgaatg atctgaatat agccaataca
    15241 aaccaataag ccgagtacag ccgttaattt tgagcgtaga taaacactca atatcagcac
    15301 taaggttcct attactatac ccacaaatag aaacgcttta ggcaaccata aaataggcag
    15361 atgaaataga atcagggcaa tacaaaaacg gtctaaagtc ataattcggc ttacttttta
    15421 aagctgagta tgccgtttat gcttaatatt ttcagaataa attttcagat tttcgacaca
    15481 gttcgcaaaa atacttgaaa aaataaaggc agacttttta gcctgcctta taaagaagag
    15541 atattatgct gatttttgat cttcagcgta acttactgct gcagtgaata atacgtctgt
    15601 tgatgaattt aatgcggttt caaccgagtc ttgaatcaca ccaatcacaa aacccacccc
    15661 aattacttga gcagcaatat cgtttggaat attaaataaa ctacaggcta atggaatcaa
    15721 taataatgaa ccgcctgcca cacctgatgc gccacaagca cagattgatg caactacgct
    15781 taatagtagt gctgttgcga aatcaggttg aataccttgt gtataagctg ccgctagggt
    15841 taatacggtt acagtaatcg ctgcacctgc catattaata cttgcaccta atggaatagc
    15901 tacagaagcg atctcatcac gaacgcccaa acgcttggct aaattcatat tcaccggaat
    15961 gttggctgct gaactgcggg taaagaatgc cgttacacca ctctctcgta aacaggtaaa
    16021 tgtaagtggg tatgggttgc ggcgaatttt ccaatacact aataatggat ttagtactaa
    16081 tgccacaaat aacattgcgc caactaagac tactaataaa cgtgcgtaat cttcaaacgc
    16141 atcaatacca ttgatggcaa cagtttcagc tactaaacca aataccccaa taggcgcaaa
    16201 tgcaatcaca actttcacaa caaacgaaac tgcgttagct aaatcattta agaatgcttt
    16261 tgaacttgcc gaaccatggc ggaatgcaat ccctaaaccg atagaccacg caagaatacc
    16321 gataaagttt gcattactta atgcgactaa tggattatca actaagttaa aaataacggt
    16381 ttttagaatt tcaccaatgc cttgtggcgg ggttaaaccg tctgggcttg cagtaagctc
    16441 taatgtagtt gggagcataa agcttaaaac aacagccgtt aatgccgcaa aaaatgtacc
    16501 taatacataa agcaccagaa tcggctttaa tttgctatct gaaccaactt ctttatttgc
    16561 aatcgcagcc aaaactaata caaatactaa aataggcgca atggcacgta gtgatttcac
    16621 aaaaaattga cctaatacac caatgctggt agcccattct ttattaataa atgccagcaa
    16681 tacccctagc actaaaccaa tggcgatacg taatactaaa ttaccgccaa gaatttttga
    16741 tgataatgat tgactcataa cccttcactt ctgtaagaca acaaaaaata aggataaata
    16801 aaatttatcc gcctgaaaaa ataggtttat aagattagcg gaaaataaac aaatatggaa
    16861 taaacaagcg gtttattttt cagatttttt tacaaaaaag tgaagttttt tacattccat
    16921 ggctatcata ttattttttc cttaggttct ttgaacatca ccattctttc agtacaatag
    16981 ttctctaaac tcttttattt attttattta ctatgaaacc aaccaataaa gcagattttc
    17041 aacatgtgat caatgcagct ggctactcta tgaaggggtt aaaagccgct tatattaatg
    17101 aagccgcctt tcgccaagaa atttggtgcg ctactatttt attccctcta gggcttattc
    17161 taggcgaaac caatattgaa aaagcattat tagtaggcac agttttactt gttttagtta
    17221 cagaactact caatagtgcg gttgaatctg ttgttgaccg cattggggct gatttccacg
    17281 agctttctgg tagagctaaa gatattggct ctgcagctgt atttatggcg atggttttac
    17341 tcgctataac ttggatatta attttgtttt gttaactcta tttttcaacc aacactctta
    17401 ctgaggtatt tatgcgctta aaaaaaacat tactttcggt agcactactt agtgcaacat
    17461 cagcaatggc ctacgagaca ggtaaaacat atcgttttac ggttttacat actaacgata
    17521 tacacggtca cttttggcaa agtgataaag gcgaatacgg tctcgctgct caaaaaacat
    17581 tggttgataa tattcgcaaa gaggtcgcag aaaaaggtgg ttcggtatta atgctgaatg
    17641 caggtgatgc gaatacaggc gtgccggagt cagatatgca aaatgcacgt cctgatattg
    17701 aaggaatgaa tgcgattggt tacgatgcaa tggttctagg taatcacgaa tttgattacc
    17761 cactgcaaat tctctctatg caagaaaaat gggctaaatt cccctttatt tcggccaatg
    17821 tcattaataa aaaaacggat aaacccattg taaaacctta cgtgatgtta gaacgaggcg
    17881 gactaaaaat tgctgttatc ggattaacaa ccgaagatac cgcaaaatta ggtaatccag
    17941 atgtaacgga aaatgccttt ttcaaaaacc ctattgaaac agcaaaaaac acattagctg
    18001 aaattaatca aaaagaaaaa cccgacgtac gtatcgcttt aacccatatg ggttactatt
    18061 ttgacggtaa acatggtact aatgcaccag gtgatgtaac aatggcccgt accttagaaa
    18121 aaggagcgtt cgatttaatc attggcggtc atacccacga tactgtttgt attgatgaaa
    18181 aaggtcaatt caaagccaaa tatacgccgg gtgaggaatg taaaccagac ttccaaaacg
    18241 gtacttggat tgtgcaagcc ggcgaatggg gcaaatttgt tggacgggct gattttgagt
    18301 ttaaagatgg aaaaactacc ttggtaaaat atgaattaat tccggtcaac ttaaaaaaaa
    18361 ccattaaaaa agaagacggt actaaagaat accgacttta ccagcctgaa atcaaagccg
    18421 atgaagcaac ctatacctta ttgaaaaaat atcaagatga gggcgataaa aagcttaata
    18481 ttgaagttgg tcgtgtgaaa gggttattag acggcaaacg tgagcatatt cgcttccaac
    18541 aaaccaatct cggtcgttta attgcacaat cgcaaatgga aagggtaaaa gccgatatcg
    18601 ggattatgaa ctccggcggt attcgtacct cccttgaaga aggtgtaata acttataaac
    18661 atattctgac tgttcaacct ttcggtaata tgattgcaac ggtggattta aaaggacaag
    18721 aattgttaga taccctcacc actattgctc tgaaagaagt tgatactggc gcttatcctc
    18781 aatttgccgg catttcaatg gtagtcgacc gtacagcaaa aactatttct gatgttaaaa
    18841 tcggtggcaa accttttgat aaagagaaaa cttataaagt ttctctgcct gactatgttg
    18901 cggctggtgg cgatggctac ccattactga aaaaacaccc tagctatgtc aatacaggct
    18961 ttattgatgc cgaaatgctg aaaaaattct tcgaagaaaa taaaattatt gaggcagata
    19021 aatacgatcc aaaaaaccag atcattttca aatagagcta tagaaaatga aactcactct
    19081 ggcagtctct gctcaaaaga gctgtggctg cctgtagatt gttttcttac tttcaagtgt
    19141 tataggaaat cgataaaaat ccctaccaaa ttaattcatc accatatgat gaaaattaaa
    19201 aaacctctgg caatactttg ccaaaagttt ttgactttta tagggtaata tcact
//
LOCUS       DANJWC010000027        19053 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.027, whole genome shotgun sequence.
ACCESSION   DANJWC010000027 DANJWC010000000
VERSION     DANJWC010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19053
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.027"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..601
                     /locus_tag="PWX02_000718"
     CDS             <1..601
                     /locus_tag="PWX02_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728752.1"
                     /translation="CVGLISGRTFLLMSVISIALYFKWKYILPLIAFTALILFLAIVF
                     PENRYVEHALEPVINLLSGTGKVSSSTDNLVKNHLFLPTLNQFISGDGLYYTETGGYY
                     GGSDSGYIRQVLYGGVSYMLACFAFTYYFVRKVAVNWFEGSWIFTLSTIGILAILNIK
                     ADTFAFPGLMMLFLMFLSLFGTSGRNVTFFCKNKEAEYV"
     gene            594..1562
                     /locus_tag="PWX02_000719"
     CDS             594..1562
                     /locus_tag="PWX02_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005578999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL1728753.1"
                     /translation="MFSIIVPSYNRNQEINALLESLKQQTAYNFEVIIVDDCSKIPVS
                     ETLGILNEFNDISTKEKVRELLTRDSLSHIYSYPFDVKVVRNNPNVGAAQSRNVGATN
                     ATQEWLLFLDDDDRFMPEKCERLAAEIANNPTANFVYHPAKCEMVNEGFTYVTKPFKH
                     KSELTKENILKANKVGGMPMIGLKKSLFCKIGGLSDRLRSLEDYDFLLKLLEEPSFEP
                     LYVDEALTYCTFHTKRSSVSTDTTNTEKAIEYIQQTYIKTPEQANNFKLNSLYILSYP
                     HVMNLSRKAAGYYFEMFKQTKNIKQLIIALVIFISPKLAINLKRFM"
     gene            1562..2362
                     /locus_tag="PWX02_000720"
     CDS             1562..2362
                     /locus_tag="PWX02_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_162680708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL1728754.1"
                     /translation="MKFSVLMSLYIKEQPQYLRECFESLKVQTVQADEIVVCFDGTVT
                     PELEAIVEEYSAILPINAVKFPQNRGLGKTLNDGLTYCKNEWVFRMDTDDICMPERFE
                     KQVAFIEQNPETVIFGGQIAEFGQDIHDLVAYRNVPTMVEEIVRFTRLRCPFNHMTVA
                     YQKSKVLEVGGYQDLQEDYYLWIKLIAQFKQVANLPDLLVYARVGNGMVGRRRGMAQA
                     EAEWRLYKLKNRCQIHDPISGFGIFIMRAIPRLLPVSALKKVYGLLRK"
     gene            complement(2399..2905)
                     /gene="msrA"
                     /locus_tag="PWX02_000721"
     CDS             complement(2399..2905)
                     /gene="msrA"
                     /locus_tag="PWX02_000721"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL1728755.1"
                     /translation="MQNIREIYLAGGCFWGVESFMQRIKGVIKAQSGYANGNTDNPSY
                     QEVCAGSGHAEVVKVTYDANQISLAKLLDYFFKVIDPVSINKQGGDIGIQYRTGIYYT
                     DPQDEPVVTAALAQLQTQFDQPLAVENQMLEQYFPAEEYHQEYLVKNPNGYCHIDIQL
                     MNEILKNQ"
     gene            3086..4348
                     /locus_tag="PWX02_000722"
     CDS             3086..4348
                     /locus_tag="PWX02_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDL1728756.1"
                     /translation="MGTLNSFLGIIVLLFIAFLFSSNKRAINVRTVLGALALQVAIGA
                     LVLYVPAGRDALNAMATGVSKVISYGNEGISFLFGGLVSDKMFEVFGGGGFVFAFRVL
                     PVIVFFSALISLLYYIGVMQWVIRILGGLLQKALGTSKAESMSAAANIFVGQTEAPLV
                     VKPYISKMTESELFAIMAGGLASIAGSVMAGYAGMGVPLTYLIAASFMAAPAGLLFAK
                     IMVPQTEKFRDELEAVDLEKPANILDAAASGASSGMQLALNVGAMLIAFVALIALVNG
                     ILGGIGGWFGYDTLNLGQIFGWVFKPLAWVIGVPWEEAEIAGQMIGLKLAVNEFVGYL
                     EFAKYLAPESTVHLTDKTVAIITFALCGFANFSSIAILIGGIGAMAPSRRGDIARLGI
                     KAVIAGSLANLMSATIAGLFIGLGGAVL"
     gene            4493..5269
                     /gene="deoC"
                     /locus_tag="PWX02_000723"
     CDS             4493..5269
                     /gene="deoC"
                     /locus_tag="PWX02_000723"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDL1728757.1"
                     /translation="MNLKKSAEIALSLMDLTTLNDNDTDEKVIALCQQANTKFGTPAA
                     ICIYPRFIPIARKALKQQGIEQVKIATVTNFPHGNDDIEIAVAETKAAVAYGADEVDV
                     VFPYKALIAGNEQIGFDLVKQCKAVCAANNVLLKVIIETGKLKSAELIRKASEISIQA
                     GADFIKTSTGKVAVNATLESARIMLETIRDLGVADRVGFKAAGGVKTSEEAAQYLALA
                     ESILGNDWVSASHFRFGASSLLANLLATLNDEQQQAVQGY"
     gene            5290..6009
                     /gene="deoD"
                     /locus_tag="PWX02_000724"
     CDS             5290..6009
                     /gene="deoD"
                     /locus_tag="PWX02_000724"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL1728758.1"
                     /translation="MATPHINAPEGAFADVVIMPGDPLRAKYIAETFLEDAKEVTNVR
                     NMLGYTGTYKGRRISVMGHGMGIPSCSIYAKELITEYGVKKIIRVGSCGAVRNDVKVR
                     DIVIGVGACTDSKVNRIRFRDNDFAAIADFDMTLAAHQAAKAKGIEARFGNLFSADLF
                     YSPDVEMFDVMEKYGILGVEMEAAGIYAVAAEYGAKALAICTVSDHIRTGEQTTSEER
                     QLTFNGMIEIALESVLIGDKQ"
     gene            complement(6085..6936)
                     /locus_tag="PWX02_000725"
     CDS             complement(6085..6936)
                     /locus_tag="PWX02_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL1728759.1"
                     /translation="MQTHRAIIESIFALAPKNELWATPISGLTVHAADRSIRFNGYIQ
                     DPSLCVVLLGERQICTNGHCQVFGNQEMMFCPVNLPIVTQTLSVAENQPYLAISLRLD
                     FDLIAKIITQLPPQKISENINQRTKWELENEIKDCLLRLLNLLNSPNHIHFLAPLILN
                     ELYFYLLQSEQGGYIRSLLSQDGNIAKIANATKILERKFADTLNMSALAEEVGMSVAG
                     FYAHFKSVTSMTPLQYQKSLRLNAAREKLQAGVSVSETAYQVGYESPSQFSREYKRQF
                     GESPKRR"
     gene            7051..7482
                     /locus_tag="PWX02_000726"
     CDS             7051..7482
                     /locus_tag="PWX02_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL1728760.1"
                     /translation="MKIFYHTSATATGGRDGRTQVDDGSIGFDLVGFQNESGKVGTNP
                     EQLFAMGYAACFDSAMNHVAPTLGIKPTKSSTTVAVGIGQKADGAFGLDLDITITVEG
                     ISAEEAKTLISKAHEVCPYSNATRGNVDVRLHVKVVSEFDL"
     gene            7499..8179
                     /locus_tag="PWX02_000727"
     CDS             7499..8179
                     /locus_tag="PWX02_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDL1728761.1"
                     /translation="MKMLDFETTVKTRQSIRKFLPLPMTETEIKQILADAQNAPSACN
                     TQPWLVHVASGETLARLKARFKKTFEAGINDADFEFNQTKFTGDYEPRWRNQYSHVFT
                     TSYGIPREDKAGRQVVYDDNIVGYGAPHMAFLFMPSIDGHDVNIASDVGMYSQTFLLS
                     LTARGFGGIPQLALAMFAKDVREELGIPEHYKLLHAISFGHPDWDAMQNKHHLGRVPV
                     EESATLHW"
     gene            8243..9367
                     /locus_tag="PWX02_000728"
     CDS             8243..9367
                     /locus_tag="PWX02_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDL1728762.1"
                     /translation="MINLFTPYTLNNDVEIKNRLVVAPMTHSASNDDGTISEQERHFL
                     HNRAADMGLFITAATGVMRNGKAFYGQPEALDERYLASLTETANILKNQGTKAILQIF
                     HGGAQAIPALLDGANAVAPSEHANGAKALTADEVKQMIQAFANATDLAIQAGFDGVEI
                     HGANGYLIQQFFSTEFNQRTDEWGGSLEKRLRFPLAVVEAAVSVREKHQRPDFIIGYR
                     FSPEEPGEHGLTMTDTYALIDALCEKPLQYLHISLWDFGKKARRGADTNLTRMQLVHQ
                     HINGRLPLIGVGNLLTGEQIRKAYATGWAEFIALGKAVMLNPNVATLLKDNRDSEIVS
                     ELDPQQADHYGIPDRLWGYCVQGGAWLPPLKGQNWQPMDI"
     gene            9451..10068
                     /locus_tag="PWX02_000729"
     CDS             9451..10068
                     /locus_tag="PWX02_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HDL1728763.1"
                     /translation="MITLHYLNSSCSHRIVWLLEALGLDYELKIYHREESGFAPMEVK
                     ALHPLGKAPVLQDGDLVLAEGNAIIQHLIARYAEGKFEPTRGSEAYSMYHYWLSISAS
                     LFSANLVIMLANRSDLGAFGDYAKAQTPLYFNYIEQTLSDKKWIVGDELTGADFSLCF
                     PLQWGRNFVEMANYPNIARYLEQVENHPAYQKANQKALKLDMNRF"
     gene            10211..11152
                     /gene="argC"
                     /locus_tag="PWX02_000730"
     CDS             10211..11152
                     /gene="argC"
                     /locus_tag="PWX02_000730"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDL1728764.1"
                     /translation="MSKYKIFVDGAVGTTGLRIHERLSQADDIELLTLPEEQRKDLNA
                     RVAKVAEADLTFLCLPDEASKELVANAPENARICDTSTAHRVNPNWVYGFPELSGQRE
                     KIQNAKRVAVPGCHATGYIALVRPLVEKGILPADYPLSCHSLTGYSGGGKAMIANYEN
                     ENSPNAFNAPRVYGLTLQHKHLPEMQAVTQMQNPPIFTPVVADYYSGMLVTVPLPIFA
                     LQAVKSGEEIANLLADYYQTSRLIHVHPFQSLPEDGMLAANAYAGKGSLEIFIYGNST
                     QLLLAARFDNLGKGASGAAVQCMNIMLGRDEVEGLEL"
     gene            11244..12098
                     /gene="argB"
                     /locus_tag="PWX02_000731"
     CDS             11244..12098
                     /gene="argB"
                     /locus_tag="PWX02_000731"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDL1728765.1"
                     /translation="MQQQEINQFANLLEKSTACLVKWQGKIIVVKYGGNAMINEELKK
                     SVMADLLLLHRLGVKVVLVHGGGPEISQGVKLLGKEPQFINGLRVTDSDTINVVLQML
                     AGKANKSLVALLKGKGIGLCGIDGAMLQCKKLEAEVDYGFVGDIVKVDPTVIEIALNA
                     GLIPVVSTVGVDENGQAYNINADTAASEIAIALQAEKLVSMTDIAGLLRDRFDESTLI
                     PEVEVSEVQSLIEQGIIAGGMIPKIACCTDFIRAGGSEANIIDGRIDHAILVEMFGGK
                     NGTRFYKK"
     gene            12111..13295
                     /locus_tag="PWX02_000732"
     CDS             12111..13295
                     /locus_tag="PWX02_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HDL1728766.1"
                     /translation="MTSSNTIKQLDKDYIAQTYGRFDLALSHGKGCEVWDFEGNKYLD
                     FTSGIGVNSLGWADEDWLQAVTTQAGKLAHTSNLFFTEPASQLAQKLVAASGLKRAFF
                     CNSGAEANEGAIKTARKYSFDKYGSGRSTVLTLVNSFHGRTISTLAATGQEVFHKHFF
                     PFTEGFEYLPVNDIEALKARLETENVCGILLEVVQGEGGVCPLEADYLHAVQSLCQEK
                     DILFMIDEVQTGIGRTGTLFAYQQFDLTPGLVTLAKGLGGGLPIGAFLLGEKVEMTLG
                     KSDHGSTFGGNPVSCAAANVVIDKINAEFLAQVQQKGQLLQQRLASLPKVKSVSGLGL
                     MLGVEFEEGILAADIVAKCINKGVLFLTAKSKLRLLPPLMISEQQIEQGIRVLEDVLK
                     DS"
     gene            13478..13909
                     /locus_tag="PWX02_000733"
     CDS             13478..13909
                     /locus_tag="PWX02_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1728767.1"
                     /translation="MSNQIALLLMSHGSFARAALASAELIVGQQENVAAIGVVAVDNV
                     ADIKRQMEQTIAELNTDKGLIILTDIVGGTPMNLASSQLTHPNVFACSGLNLPLLLEV
                     LMSRDGNLQEIRENLPLAYQNGFSLRTQQDFVSQEDDDDLL"
     gene            13896..14363
                     /locus_tag="PWX02_000734"
     CDS             13896..14363
                     /locus_tag="PWX02_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1728768.1"
                     /translation="MICFSRIDDRLIHGQVVTTWVNLHRIEQIIVLNDAVAADPTQKQ
                     ILTMAAPQGIKVVAFGIEKFGEVLQKTQITRRTMLLFTNSIDVLRTVELGVSIDKLNV
                     GGMRYQAGREQLSKAVSVTPEERQAFKTLLEKGVEVTVQMVPNDEKLNLQEII"
     gene            14366..15133
                     /locus_tag="PWX02_000735"
     CDS             14366..15133
                     /locus_tag="PWX02_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1728769.1"
                     /translation="MLTALLVACWAGICALDDIGTQMIRRPLLIAPVVGLIMGDLETA
                     LIIGATLEVMWMGIGNVGAYSAPDMISGTAIGTALGIAAGEASTAVALAVPTSLLAQQ
                     LLILYRSGVCALNPIAERWAASGDFDKIFRIHYIPMLIAFSIRAIPTFLAVYYGADII
                     AQAVSALPAKVITGLNVAGKIIPAIGIGLLMLMMIKKAELWTFLIAGFTLAVYLKLSV
                     LPITLIALPLALIYDLAVSKKAEAAPTKTADEEDYDL"
     gene            15130..15936
                     /locus_tag="PWX02_000736"
     CDS             15130..15936
                     /locus_tag="PWX02_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL1728770.1"
                     /translation="MSNKITKQDLNKVFWRMQVLNVTNNFQSMQAIGFLSSFIPVLKR
                     LYKDRPQEERAEAMKRHLKFFNSHVNSDALILGIAAAVEETTKEDEKETVTAIKTGLM
                     GPLAGLGDSLLKFTWLPICGSIGAALALSGNVLGPILMFLLYNSVNIATKYYGIHLGY
                     SKGVDLIHGAGNNILQRLGNMANVVGLMVIGALIASVVKVKVVSQIAAGDNVIKFQEM
                     LDKVMPGLLSLLLTVVIYWILKKTNGKYAATMILATMGICIGLVYAGILG"
     gene            15936..16973
                     /locus_tag="PWX02_000737"
     CDS             15936..16973
                     /locus_tag="PWX02_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HDL1728771.1"
                     /translation="MDSVYDIIRLEQGRYLFRQQPAQSVQIFFLDNPDSKDSKWLAET
                     DLAEFELKLSACEARPFFLIQSANGEQIVAERTLPVAGMNNFRDMGGYVAHQGKRVKW
                     GKLYRSDHLHNLRDEGVAYLDKLGIQTVIDYRSPNEVAKYPNPPINGREQTFRLDPNA
                     HTAELAAQFSADKHDEDRNLVNKIIAQKAEGNLINRYDIVMAQYRNFVEKAECQTAFA
                     EMLRLATDPENAPLVQHCRGGKDRTGFGAMLLLGILGVSKADIIADYMLTHDNRLARN
                     EEKMAIYRTFTQDQDVLDYLLSLIDTQPEFIEQSLNTIETQYGTIEQYAQRVLGITAK
                     EIEALRANYLA"
     gene            17006..18073
                     /locus_tag="PWX02_000738"
     CDS             17006..18073
                     /locus_tag="PWX02_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine-type phosphatase"
                     /protein_id="HDL1728772.1"
                     /translation="MEKFHMRYFAKHWYKTTACLTTIFTAFPLFAQPIENIPTSHYEL
                     EKIIIFSRHGLRSPLTEKGSDLEKSTPYKWANWSVPTGHLTHKGTLLETYFGQYLGQW
                     LTHNGLLDKASCESGKQIEVYANSLQRTIGTAQALVAGGFSGCNVQVKHHQQIGKMDP
                     VFNPIIRSDDQTFVQTALSKIDLNKTHQYLAPSYHLLSEIIDYAHSEKCLQEKQCEFF
                     NDVGKLRIKKDKEPGVSGTMRLSENIVDALLLQYYEGLPLKEIANNQLDSDQKWQTLN
                     KIKNDYQFMLRGDPFIAKHISLPLLKYIQQDLNSENKITLLVGHDSNIIALLSALNVK
                     PYKLAHQYEQTSIGGKVFIEI"
     gene            18591..>19053
                     /locus_tag="PWX02_000739"
     CDS             18591..>19053
                     /locus_tag="PWX02_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL1728773.1"
                     /translation="MSQDIQADLKKILALLSRNRRFQSLFLLLLRSEQFINSDALSTE
                     LQLSVRTVKKEIKLLKEKLEPYGIQIFSRPFYGYKLLIQFEELHTQLKQYFQISQPMT
                     VNNKFESRVNAILRRLLVSQRALKVEDFQSELYLSFTSALTKEFSQVKALLG"
BASE COUNT         5567 a         3645 c         4280 g         5561 t
ORIGIN      
        1 atgtgttggt ttaatttccg gtagaacctt tttattaatg tcagtaataa gtattgcact
       61 ctattttaaa tggaaatata tattgccact gattgccttc actgcgttaa ttctattttt
      121 ggcgattgtt tttccggaaa accgttatgt tgaacacgcc ttagaaccgg taattaattt
      181 attgtccggc accggaaaag tcagctcttc aaccgataat ttagtgaaaa accatctgtt
      241 tttaccgact ttaaatcagt ttatttccgg tgatggttta tattacaccg aaactggtgg
      301 ctattatggt ggatcggatt ccggctatat tcgccaagta ttatatggtg gagtaagtta
      361 tatgttggcg tgttttgcct tcacttacta ttttgtacgc aaagtggcgg tgaattggtt
      421 tgaaggcagt tggattttta ccttatccac catcggcatt ttagctattt taaatattaa
      481 agcagatacc tttgctttcc cggggctgat gatgctgttt ctaatgttct tgtcgctgtt
      541 tggaacaagc ggtcgaaatg taacattttt ttgcaaaaac aaagaggctg aatatgttta
      601 gtattattgt gccttcttat aaccgtaatc aagaaatcaa tgcgttgctt gagagtttaa
      661 agcaacaaac ggcatataat tttgaagtga tcattgttga tgactgctca aaaataccgg
      721 tcagtgaaac tttaggaatt ttaaatgaat ttaacgatat ttctactaaa gaaaaagtaa
      781 gagaattact tactagagat agtttatctc atatttatag ctatccattt gatgtgaaag
      841 tggtgcgaaa taacccaaat gttggggcgg cacaaagccg aaatgtaggg gcaactaatg
      901 ccacacaaga atggctactg tttttagatg atgatgaccg ctttatgcct gaaaaatgcg
      961 aacgtttagc ggcagagatt gcgaacaatc caactgccaa ttttgtgtac cacccggcaa
     1021 aatgtgaaat ggtaaatgag ggttttactt atgtcaccaa gccgtttaaa cataaaagtg
     1081 agctaactaa agaaaacatt ctaaaagcga ataaagttgg cggaatgccg atgattggct
     1141 tgaaaaaatc actcttttgt aaaattggtg gattatctga ccgcttgcga tcacttgaag
     1201 attatgattt cttactgaaa ttattggaag aaccgagttt tgagccattg tatgtggacg
     1261 aagccttgac ctattgtact ttccatacca aacgctccag tgtttcaacc gataccacta
     1321 atacagaaaa ggcgattgag tatattcaac aaacgtatat caaaacacct gagcaggcga
     1381 ataattttaa gctcaattcg ctttatattt taagttatcc acacgtaatg aatttatccc
     1441 gcaaggcagc cggttattat tttgagatgt ttaaacagac taaaaatatc aaacaactga
     1501 ttattgcgtt ggttattttt atttcgccga agttggcgat taatttgaaa aggtttatgt
     1561 aatgaaattt tccgttttaa tgtcgttata cattaaagag caaccgcaat atttaagaga
     1621 atgttttgaa agcttgaaag tacaaactgt gcaggctgat gaaatcgttg tttgttttga
     1681 tggtacagtt acgcctgaat tagaggcgat tgtagaagaa tatagtgcaa ttttgccgat
     1741 taatgcggtg aaattcccac aaaatcgagg tttaggtaaa acgctaaatg atgggctaac
     1801 ctattgtaag aatgaatggg ttttcagaat ggatacagac gatatttgta tgccggaacg
     1861 tttcgagaaa caagtggcat ttattgaaca gaatcctgaa accgtgattt ttggcggcca
     1921 aattgctgaa tttgggcaag atattcacga tcttgtcgct taccgcaatg taccaaccat
     1981 ggtggaggaa atcgtcagat tcactcgctt gcgttgcccg tttaaccata tgacggttgc
     2041 ttatcagaaa tccaaagtgt tggaagtcgg tggctatcaa gatctgcaag aagattacta
     2101 tttatggatc aaactcattg ctcaatttaa acaagtggca aacttgccgg atttattagt
     2161 gtatgcccga gtcggtaacg gaatggttgg cagacgccgt ggaatggccc aagcagaggc
     2221 agaatggcgt ttatataaat taaaaaaccg ctgccagatt cacgatccga tttcaggttt
     2281 tgggattttc ataatgcgtg caattcctcg tttattaccg gtttcagcac tgaaaaaagt
     2341 gtacggtttg ctcagaaaat aaagctaaaa aaaccacaga ttgctctgtg gttttttgtt
     2401 attgattctt caaaatctca ttcattagtt gaatatcaat atggcagtag ccgttcgggt
     2461 tttttactaa atattcttgg tggtactctt cggcagggaa atattgctct aacatttggt
     2521 tttccactgc cagcggctga tcaaattggg tttgtaattg tgcaagtgcg gcggttacca
     2581 ccggttcgtc ttgtgggtcg gtataataaa taccggtgcg gtattgaata ccgatgtcgc
     2641 caccttgttt gttaatgctc accgggtcta ttactttaaa gaagtaatct agtaattttg
     2701 ccaggctaat ttgattggca tcataagtca cttttaccac ttcagcgtgt ccgctaccgg
     2761 cacacacctc ttgatagctt gggttatcgg tattaccgtt ggcatagcct gattgtgcct
     2821 taatgacacc tttaatccgt tgcataaaag attccacgcc ccagaaacag ccaccggcta
     2881 agtaaatttc acgaatattt tgcatattac ctctctataa ttgtaaattt ttcgataaat
     2941 ttgactgctt gtgatgaata ataaaatacc ataatttagt ttttgtcaaa ttttgtcttg
     3001 aatgatgata atttcacaag attttttgcc taaaaatagg tatagtatcg tcaccttttt
     3061 taatcaattt ataattagag gttctatggg tactcttaac agctttttag gcattattgt
     3121 tttactattc attgcatttt tattttcgag caataaacgt gcaataaacg tgcgtaccgt
     3181 attaggcgca ttagcattgc aggtagctat tggtgcattg gtgctttatg tgccggcagg
     3241 gcgtgatgcc ttgaatgcga tggcaaccgg tgtgtcaaaa gtgatttctt acggtaatga
     3301 agggatttca tttttattcg gtggcttagt cagcgacaaa atgtttgaag tatttggcgg
     3361 cggcggcttt gtgttcgctt tccgtgtact tccggtgatt gttttcttct ctgcactcat
     3421 ttctttgctt tactacattg gtgtgatgca atgggtaatc cgtattttag gtggtttatt
     3481 acaaaaagca ttaggtacat ctaaagcgga atctatgtct gcggcagcga atattttcgt
     3541 tggtcaaact gaagctccat tggtagtaaa accttatatc agcaaaatga cagaatctga
     3601 actctttgcg attatggcag ggggcttagc ttctattgca ggttctgtta tggctggtta
     3661 cgcaggaatg ggcgtaccat taacttactt aatcgcagcc tcctttatgg cggctccggc
     3721 agggttatta ttcgccaaaa ttatggtacc acaaaccgaa aaattccgtg atgaactaga
     3781 agcggtagat ttagaaaaac cggcaaatat tcttgatgca gcagcttcag gtgcttcttc
     3841 aggtatgcaa ctggcattaa acgtaggggc aatgttaatt gcattcgtgg cgttaattgc
     3901 attagtgaat ggtattttag gcggtattgg cggctggttc ggttatgata ccttaaactt
     3961 aggtcaaatt ttcggctggg tatttaaacc gcttgcgtgg gtaatcggtg tgccttggga
     4021 agaagcagaa attgcggggc agatgatcgg cttaaaatta gcggtaaatg aattcgtggg
     4081 ttatttagag tttgctaaat acttagctcc tgagtcaacc gtacatttaa cggataaaac
     4141 tgttgcgatt attacctttg cactttgtgg ctttgcgaac ttcagctcga ttgcgatttt
     4201 aatcggtggt atcggggcaa tggctccaag ccgtcgtggt gatattgccc gacttgggat
     4261 taaagccgtc attgctggct cacttgccaa cttaatgagt gcaaccattg ccggcttgtt
     4321 tatcggcttg ggtggagcag ttctctaatc cagtttgtat aaaaccacta gaaatagtgg
     4381 ttttatcttt ttttatctga cgtaaacggt tgcttttggt ttgtctattt acaagcggtc
     4441 agatttttat aaaattttac caaccaccac gcatttaata aaaggagaca ctatgaattt
     4501 aaaaaaatcc gctgagattg ctctttcgtt gatggatctc accacattaa atgacaatga
     4561 tactgatgag aaagtcatcg cattatgcca acaagcgaac accaagtttg gcacaccggc
     4621 agcaatttgc atttatccac gttttatccc gattgcccgc aaagcattga aacagcaagg
     4681 tatcgagcaa gtaaaaattg cgacagtcac gaatttccct cacggtaatg atgatattga
     4741 aattgcggta gctgagacca aagccgcagt tgcttatggg gcagatgagg tagatgtggt
     4801 attcccttat aaagcattaa ttgcaggtaa tgagcaaata ggtttcgact tggtaaaaca
     4861 atgcaaagca gtttgtgctg ccaataatgt gttgcttaaa gtgattattg aaaccggcaa
     4921 gttaaaatca gcagagctta ttcgcaaagc aagcgaaatt tcgattcaag caggggctga
     4981 ttttattaaa acctcaaccg gcaaggtggc agtgaatgct acccttgaat ctgcccgcat
     5041 tatgcttgaa accattcgtg atttaggtgt ggcagaccgt gttggcttta aagctgcagg
     5101 tggggtaaaa acctcggaag aagccgccca atatcttgca cttgcggaat ctattttagg
     5161 caatgactgg gtgagtgcat cacattttcg cttcggtgca tcaagtttat tagctaattt
     5221 attagcaaca ttgaatgatg aacagcagca agctgttcaa ggttattaat taataaagag
     5281 gaacataaaa tggctactcc acacattaac gcacctgaag gtgctttcgc tgacgttgta
     5341 attatgccgg gcgatccgct tcgtgctaaa tatatcgctg aaaccttttt agaagatgca
     5401 aaagaagtga ccaatgttcg caatatgtta ggctacaccg gcacttataa aggtcgccgc
     5461 atttcagtaa tggggcacgg tatggggatt ccatcttgct cgatttacgc taaagaatta
     5521 attactgaat atggcgtgaa gaaaattatc cgtgtcggct cgtgcggtgc ggtacgtaat
     5581 gatgtcaaag tgcgtgatat tgtcatcggt gttggggctt gtaccgattc taaagtaaac
     5641 cgtattcgct tccgtgataa cgattttgcg gcgattgctg attttgatat gacattagct
     5701 gctcaccaag cagctaaagc gaaaggcatt gaagctcgtt tcggtaattt attctcagcc
     5761 gatttattct actcacctga tgtcgaaatg tttgatgtaa tggaaaaata cggcatttta
     5821 ggcgtagaaa tggaagcagc aggcatttat gcggtagcgg cagagtatgg agcaaaagca
     5881 ttagcaattt gcaccgtttc agaccatatc cgcaccggtg agcaaactac ctctgaagaa
     5941 cgtcaattaa cctttaatgg tatgattgaa attgcgttag agtcagtatt aattggcgat
     6001 aagcagtaat ttctaacctg acaagcggtg aaattttgca aaaaatttgc agaatttgac
     6061 cgcttgttat ttttgattct tcctttatct tcttttcggg ctttcgccaa actgcctttt
     6121 atattctcga ctaaactggc ttgggctttc atagccgact tgataggcgg tttcactcac
     6181 gcttactcct gcttgtagtt tttcccttgc cgcatttagt cttaacgatt tctgatattg
     6241 cagtggtgtc attgaagtta cgcttttaaa atgagcgtaa aaccccgcca cgctcattcc
     6301 tacctcttca gccagtgctg acatatttag tgtatcggca aattttcttt ctaagatttt
     6361 tgttgcattg gcaattttag caatattgcc atcttgagag agtagagagc gtatatagcc
     6421 accttgctca gattgcaata aatagaaata gagttcgttt aatatcaatg gtgcaagaaa
     6481 atgaatatga tttggagaat taagcaaatt tagtaagcgt aacaaacagt ctttaatttc
     6541 attctccagc tcccatttag tgcgttgatt aatgttttca cttattttct gtgggggaag
     6601 ctgcgtgata attttggcaa taagatcaaa atccaagcgt agtgaaatcg caagataagg
     6661 ctgattttct gcaactgata aggtttgggt aacaatcggc aaattcacag ggcaaaacat
     6721 catttcctga ttaccgaaga cttggcaatg tccattggta cagatttgcc gctcgcctaa
     6781 taatactaca cataaacttg ggtcttggat ataaccgtta aatctaatag aacgatcggc
     6841 agcgtgaacg gttagccctg aaatgggagt agcccaaagt tcatttttcg gagcgagggc
     6901 aaaaatagat tcaataatag ctcgatgagt ttgcataata atctcacttt tagaaaatta
     6961 ggcatatttt atagaaaatc aggcaagacg acaaatagca aaaagcggat aatatcccca
     7021 atcaaataaa gcatttaata ggagcaaaat atgaaaattt tttatcacac atcagcaact
     7081 gcaaccggtg gtcgtgacgg cagaacccaa gtggatgacg gttctatcgg ttttgattta
     7141 gtcggtttcc aaaatgaaag tggcaaagta ggcactaatc cggaacaatt attcgcaatg
     7201 ggctatgctg cctgctttga cagtgcaatg aatcacgttg ccccaacctt aggcattaag
     7261 ccaaccaaat ccagtaccac tgttgcagtt gggattggtc aaaaagctga tggtgctttc
     7321 ggcttagatt tagatattac cattactgtg gaaggcatta gtgcggaaga agcgaaaact
     7381 ttaattagca aggcacacga agtttgccca tattcaaatg caacccgtgg taatgtagat
     7441 gtgcgtttac acgttaaagt cgtgagtgaa tttgacttat aaaaaataaa taggggaaat
     7501 gaaaatgtta gattttgaaa ccacggtaaa aacacgacaa tcaatccgca agtttttacc
     7561 gcttccgatg acggaaactg aaatcaaaca aattcttgcc gatgcacaaa atgccccttc
     7621 ggcttgtaat acacagccgt ggttagtaca cgttgcctct ggtgaaacgc tagcacgctt
     7681 aaaagctcgt tttaaaaaga cttttgaagc gggcattaat gatgcggatt ttgagttcaa
     7741 tcaaacgaaa tttaccggtg actacgaacc acgttggcgt aaccaataca gccacgtttt
     7801 taccaccagc tatggcattc cccgtgaaga taaagccggt cgccaagtgg tgtatgatga
     7861 caatattgtc ggttatggtg cgccacatat ggcattccta tttatgccaa gcattgacgg
     7921 acacgatgtc aatattgcca gcgatgttgg tatgtattcg caaactttct tgctctcact
     7981 caccgctcgt ggttttggcg gcattccgca gcttgccctt gcgatgtttg ctaaagatgt
     8041 gcgtgaagag ttaggcattc ctgaacatta caagctcctc cacgccattt ctttcggaca
     8101 cccagattgg gacgctatgc agaacaaaca ccatttaggg cgtgtgccgg tggaagagag
     8161 tgcgacctta cattggtaat aaacaagcgg ttgatttttc gcaaaaattt gcgaaatcaa
     8221 ccgtattttt taggagaata cgatgataaa tttatttacc ccttatactc tgaataacga
     8281 tgtagaaatt aaaaaccgcc ttgtagttgc cccaatgacc cattccgcct ccaacgatga
     8341 cggcacaatt agcgagcaag aacgccattt cttgcataat cgtgcagcag atatgggctt
     8401 atttatcacc gcagcaaccg gggtaatgcg taacggcaaa gcgttttacg gtcagccgga
     8461 agctcttgat gaacgctatt tggcaagcct gacagaaact gccaatatct tgaaaaatca
     8521 aggcaccaaa gcgattttgc aaattttcca cggtggggca caagctattc ctgccttgct
     8581 tgacggggcg aatgcagttg cgccgtccga gcacgccaac ggtgctaaag ccctaacggc
     8641 ggacgaagtg aagcaaatga tccaagcttt cgctaatgcc accgatttag ctattcaagc
     8701 cggttttgat ggtgtggaaa ttcacggggc gaacggctat ttaattcaac aatttttctc
     8761 tactgaattt aatcaacgca ctgatgaatg gggcggtagc cttgaaaaac gcttgcgttt
     8821 cccgttagcg gtggttgagg cagcggtcag cgtgcgtgaa aaacaccaac gccctgattt
     8881 tattatcggc taccgtttct caccggaaga gccgggcgaa cacggcttaa ccatgaccga
     8941 tacctacgcc ctgattgatg ctttgtgcga aaagccgttg caatacttgc acatttcact
     9001 ctgggatttc ggcaaaaaag cccgccgtgg tgcagatacc aacctcaccc gtatgcaact
     9061 ggtacaccaa cacatcaacg ggcgtttacc gctgatcggc gtgggtaact tattaaccgg
     9121 tgaacagatc cgtaaagctt atgcaacagg ctgggcagaa tttatcgcct taggtaaagc
     9181 ggtaatgctc aacccgaacg tggcgacctt gttgaaagat aatcgagaca gtgagattgt
     9241 cagcgaactc gacccgcaac aagccgacca ctacggcatt cccgatagac tatggggcta
     9301 ctgcgtgcaa ggtggtgctt ggttaccgcc gcttaaaggg caaaactggc aaccaatgga
     9361 tatttaatca ttacagacaa gcggtcattt ttttgtaaaa atttgcaatt aagtgaccgc
     9421 attttttcaa ttatctgaag gagaaaaatt atgattacgt tacattacct gaacagttcc
     9481 tgctctcacc gcatcgtttg gttgttggaa gccttaggct tggattacga gctaaaaatc
     9541 taccatcgtg aagagagcgg ttttgcacct atggaggtaa aagccttgca cccacttggc
     9601 aaagcccctg tgttgcaaga tggcgatttg gtgcttgccg aaggcaatgc gattatccag
     9661 catctgattg cccgttacgc cgaagggaaa tttgagccga ctcgtggtag cgaagcctat
     9721 tcaatgtatc actactggtt gtcgatttct gcttcattat tctcggcaaa ccttgtgata
     9781 atgttggcta atcgctctga tctcggagct tttggcgact acgcaaaagc gcagactccg
     9841 ctgtatttta actatattga gcaaacactt tcagataaaa aatggattgt gggtgatgaa
     9901 ctaaccggtg cggatttttc tctctgtttc ccactacaat gggggcgtaa ttttgttgaa
     9961 atggcaaatt acccgaatat tgcacgctat ttagaacagg tggaaaatca ccctgcttac
    10021 caaaaagcga atcaaaaagc cctgaagctt gatatgaata gattttaata ctctttttaa
    10081 tccctaacat tttgttaggg atttttgaat ataaaatcaa ttaatttgaa taattattca
    10141 aaaataaact tgagtttagc cttaaatagc cttagactac cgagcagaaa aagtcaaaga
    10201 aggattttca atgagcaaat acaagatttt tgttgatggg gcagtaggaa caaccgggct
    10261 acgcattcac gaacgtttaa gccaagcgga cgatattgaa ttattaacct tgcccgaaga
    10321 gcagcggaaa gatttgaacg cccgtgtggc aaaagtcgca gaggcggatt taactttcct
    10381 ctgtttgccg gatgaagcct caaaagaatt agtagcaaat gcaccggaaa atgcccgaat
    10441 ttgcgatact tcaaccgctc atcgggtcaa tcctaattgg gtttatggtt ttccggaatt
    10501 gagcggacaa cgtgaaaaaa tccaaaatgc caagcgtgtt gcggtgccgg gttgccatgc
    10561 cacgggctat attgcgttgg ttcgtccact ggtggaaaaa gggattctgc ccgccgatta
    10621 ccctttaagt tgtcattcct taaccggcta ttcaggcggt ggcaaagcga tgattgctaa
    10681 ctatgaaaat gaaaacagcc caaatgcttt caatgcccct cgtgtttacg gtttaactct
    10741 ccaacacaaa catttgcctg aaatgcaggc agttacccaa atgcaaaacc caccgatttt
    10801 cacccctgtg gttgccgatt attacagcgg aatgttagtc actgtgccat tgccgatctt
    10861 cgccttacaa gcggtcaaat ccggcgaaga aattgcaaat ttgctcgccg attactacca
    10921 aaccagccga ctaattcacg ttcacccttt ccaatctcta cccgaagacg gaatgcttgc
    10981 cgcgaatgct tatgcaggta aaggcagctt agagattttt atttacggca acagcaccca
    11041 acttttactt gctgcccgtt ttgacaattt aggcaaaggg gcatcaggtg ccgcagtgca
    11101 gtgtatgaat attatgttgg gaagagatga ggttgagggg ttagagcttt aaatataaat
    11161 gtagggacgg gtcctgtgcc cgtccgaata tgggcgatga ttatcggaca ggcacaggac
    11221 ctgtccctac aagagaaaga aatatgcaac aacaagaaat caatcaattt gcaaatttat
    11281 tagaaaaatc caccgcttgc ttggtgaaat ggcagggtaa aatcattgtg gtgaaatatg
    11341 gcggtaacgc aatgattaat gaggaactga aaaaatcagt gatggcagat ctgctgttac
    11401 ttcatcgttt aggcgtaaaa gtggtactgg tacacggtgg cgggccggag atttcacaag
    11461 gcgtgaaatt attgggtaaa gaaccgcaat ttatcaacgg gttgcgtgtt accgatagcg
    11521 atacgattaa cgtggttttg caaatgttgg caggcaaagc taataaaagc cttgtcgcct
    11581 tattaaaagg gaaaggcatt gggctgtgtg gtattgatgg ggcgatgttg caatgcaaaa
    11641 aattagaggc agaggtggat tacggctttg tgggcgatat tgtgaaagtc gatccaactg
    11701 tgattgaaat tgctcttaat gcaggcttga ttccggttgt ttccacggtg ggcgttgatg
    11761 aaaacgggca ggcttataac attaatgcag acaccgctgc cagcgagatt gcgattgcgt
    11821 tacaagcgga aaaattggtc tcgatgaccg atattgcagg tttattgcga gatcgctttg
    11881 atgaaagcac cttaattccg gaagtggaag taagcgaagt gcagtccctg attgagcaag
    11941 gcattattgc cggtgggatg atccccaaaa ttgcgtgctg taccgatttc atcagagctg
    12001 gtggctctga ggcaaatatt attgacgggc gaatcgacca cgccattttg gtagaaatgt
    12061 tcggtggcaa aaacggcaca cggttttata aaaaataaca ggaaagtgaa atgacaagca
    12121 gtaatacgat taagcaatta gataaggatt atatcgccca aacctatggc agatttgacc
    12181 ttgcactgtc gcacggaaaa ggctgtgaag tgtgggattt tgagggcaat aagtatcttg
    12241 attttaccag tggcattggg gtaaatagtt taggctgggc ggatgaggat tggctacaag
    12301 ctgttaccac acaagccggc aaattagctc acacctcaaa cctgtttttt accgaaccgg
    12361 caagccaatt agcccaaaaa ttagtggctg caagcggtct aaaacgggca tttttttgca
    12421 attcaggggc agaggcaaat gaaggagcaa ttaaaaccgc ccgcaaatat agtttcgaca
    12481 aatacggttc cggtcgttca acggttttaa ccctcgtgaa ctcttttcac ggacgaacca
    12541 tttcaaccct cgcagccact gggcaagagg tattccacaa gcattttttc ccctttacag
    12601 agggctttga atatttacct gtaaatgaca ttgaggcact gaaagctcgc ttggaaactg
    12661 agaacgtttg cgggattttg ctggaggtgg tgcagggcga gggcggtgtt tgcccattag
    12721 aggcggatta tctgcacgcc gtgcaaagcc tgtgccaaga aaaagatatt ttatttatga
    12781 ttgatgaggt gcaaaccggc attgggcgaa cgggcacact ttttgcttat caacaatttg
    12841 atttaactcc tggtttagtc acgctcgcta aaggcttggg cggcggcttg ccgattggtg
    12901 ctttcctgtt aggcgaaaaa gtggaaatga cattaggcaa aagcgaccac ggctcaacct
    12961 ttggtggcaa tcctgtttcc tgtgctgctg caaatgtagt aatcgataaa ataaacgccg
    13021 aatttctcgc ccaagtgcaa caaaaagggc agttgttaca gcaacgctta gcgagcttac
    13081 caaaagttaa atccgtttca ggcttaggtt taatgctcgg cgtagaattt gaggaaggca
    13141 ttttagccgc tgatattgtt gcaaaatgta tcaacaaagg tgtgctgttt ttaaccgcta
    13201 aaagtaagtt aaggttactc ccaccgctga tgatctctga acaacagata gaacaaggaa
    13261 tcagagtgtt agaggatgtc ttgaaagatt cttgagttat tataaacagt aggaatatct
    13321 tcttactgtt ttttattact attatatagt agcctacttt aatctgttgt cactttatat
    13381 ttcttcttat gtgtacgata tatcgtgcaa taacttccct tgtttatctg tataacattt
    13441 tatttaatag aatgacttat aaatcaagga gtgacttatg tctaatcaaa ttgcgttatt
    13501 gttgatgagc cacggcagtt ttgcgagggc ggcgttggcg agtgccgagc tgattgtcgg
    13561 gcagcaggaa aatgtggcag ctatcggcgt agtagcagtg gataatgtag cggacattaa
    13621 acggcagatg gagcaaacca ttgctgagtt aaacaccgac aaaggcttga ttattctcac
    13681 cgatattgtt ggtggtacgc cgatgaatct tgcctcttct caattaaccc accctaatgt
    13741 ttttgcctgt tcgggtttga atttaccttt attgctagaa gtgctgatga gccgtgacgg
    13801 caatttgcaa gaaattcggg aaaatctacc gcttgcatac caaaacggat ttagcctacg
    13861 cacacaacaa gattttgttt cacaggagga cgatgatgat ctgctttagc cgtattgatg
    13921 accgtttaat tcacggtcaa gtagtcacca cttgggttaa tttacaccgt attgaacaaa
    13981 ttattgtgtt aaatgatgcg gtggcagcgg atccaaccca aaaacagatt ttaacgatgg
    14041 ctgcaccgca gggaattaaa gtggtcgcgt ttggcattga aaaatttggt gaagtgttgc
    14101 agaagacaca aattacccgc cgtactatgt tgttgtttac caatagcatt gatgtgttac
    14161 gcactgtaga actaggtgta tcgattgaca aattgaatgt cggcggaatg cgttatcaag
    14221 ctgggcgtga gcagttaagt aaagcggttt cagttacgcc agaagagcgt caagcattta
    14281 aaaccttatt ggaaaagggt gttgaagtaa cggtacagat ggtaccgaat gatgaaaaac
    14341 ttaacttaca ggagattatc tagctatgct gactgcacta cttgttgcct gctgggcagg
    14401 tatttgtgcc ttagatgata ttggcacaca gatgatccgc cgtccgttgt taatcgcacc
    14461 ggtggtcgga ttgattatgg gggatttgga aacggcgttg attattggtg ctacgcttga
    14521 agtgatgtgg atggggatcg gcaatgttgg tgcatattct gcaccggata tgatttccgg
    14581 aaccgctatt ggcaccgcac ttggtattgc tgctggtgaa gcttctactg cggttgcact
    14641 cgcagtaccg acctcactct tggcacagca gttactcatt ctttaccgct ccggcgtatg
    14701 tgcattgaac ccaattgctg aacgctgggc ggcaagcggt gattttgaca agattttccg
    14761 cattcactat atcccaatgt taattgcctt ttctattcgt gctattccaa cgttcttagc
    14821 cgtctattac ggggcggaca ttattgccca agcggtctct gctctgccgg caaaagtgat
    14881 taccggatta aatgttgcag gcaaaattat tcctgccatc ggtatcggtt tgctgatgtt
    14941 aatgatgatt aagaaagccg aactttggac gttcttaatt gctggcttta cgttggcggt
    15001 ttatctcaaa ttatcagtat tgccgattac cttaattgca ctaccgcttg cgttgattta
    15061 cgacttagca gtaagcaaaa aagctgaagc tgcaccaacc aaaaccgcag atgaagagga
    15121 ttatgaccta tgagcaataa aattaccaaa caagatttaa acaaagtgtt ttggcggatg
    15181 caggtgctga acgtcacaaa caacttccaa tcgatgcagg cgattggatt tttaagtagc
    15241 tttattccgg tattaaaacg actttataaa gatcgtccac aggaagaacg agcggaagca
    15301 atgaaacgcc acttaaaatt ttttaacagc cacgttaatt ctgatgcatt gattttagga
    15361 attgccgctg cggtagaaga gactaccaaa gaggatgaaa aagagacagt aaccgcgatt
    15421 aaaaccggtt taatggggcc gttagcaggg ctgggagaca gcttgttgaa attcacctgg
    15481 ttaccgattt gcggtagtat cggggcggca ttggcactaa gtggcaatgt gttaggcccg
    15541 attttaatgt tcttgttgta taacagtgta aacattgcga cgaagtatta cggtatccat
    15601 ctaggttaca gcaaaggggt tgatttaatt cacggtgcgg gtaataacat tttgcaacga
    15661 ctcggcaata tggcaaatgt ggttggttta atggtgattg gcgcattaat tgcctccgtt
    15721 gtgaaagtca aagtagtttc acaaattgcc gctggtgata atgtgattaa attccaagag
    15781 atgttagata aagtgatgcc gggactactc agcttacttt taactgtcgt gatctactgg
    15841 attttgaaga aaactaacgg caaatatgct gcaacgatga tccttgctac gatggggatc
    15901 tgtatcggct tagtctatgc aggaatactc ggctaatgga cagtgtgtat gacattattc
    15961 ggttggaaca gggcagatac ctgttccgcc aacagccggc tcaatcggta cagattttct
    16021 ttctggacaa ccctgacagt aaggatagca aatggctggc ggagactgat ttggcagagt
    16081 ttgagctgaa attgagcgct tgtgaagccc gtccgttctt cctcattcaa tcagcaaacg
    16141 gagaacagat tgttgctgaa cgcaccttac ctgttgccgg aatgaataat tttcgggata
    16201 tggggggcta tgtcgctcat cagggcaagc gagtgaaatg gggcaagtta taccgctccg
    16261 atcatctgca taatttaaga gacgaaggcg tggcttatct tgataaattg ggaattcaga
    16321 ctgttattga ctatcgcagc ccgaatgaag tcgccaaata cccaaatccc ccaattaatg
    16381 gcagagagca aacttttcgg cttgatccga atgcacatac tgccgagctt gctgcccaat
    16441 ttagtgcaga caagcacgat gaagatcgta atttggtcaa taaaatcatt gctcaaaaag
    16501 ctgaaggtaa tcttatcaat cgctacgaca ttgtaatggc acaatatcgt aattttgtcg
    16561 aaaaagcaga atgccaaacc gcctttgctg aaatgttaag gttggcgaca gaccctgaaa
    16621 atgcaccatt ggttcaacac tgccgtggag ggaaagatcg cactggtttc ggtgcaatgt
    16681 tactgcttgg tattttaggc gtgagtaaag ctgatattat tgctgattat atgcttactc
    16741 atgataaccg ccttgctcgt aacgaagaga aaatggcgat ttaccgtacc ttcactcaag
    16801 atcaagatgt gttggactac ctattgtctt taatcgatac tcaacccgaa tttatcgaac
    16861 aatcgcttaa tactattgaa acccaatatg gcactattga gcaatatgcc caaagggtat
    16921 taggtattac tgcgaaggaa attgaagcat tacgggcaaa ttatttggcg tagaacggta
    16981 gggtgtaata ttgaacacac tacaaatgga gaaatttcat atgcgttatt ttgcaaaaca
    17041 ttggtataaa actaccgctt gtctgacaac aattttcaca gcatttcctc tttttgctca
    17101 accaattgaa aatataccaa cttcccacta cgaattagaa aaaatcatta tatttagccg
    17161 acacgggtta cgttcccctc ttacagaaaa aggcagtgat ttagaaaaat caacccctta
    17221 taaatgggca aattggagtg taccgaccgg tcatttaacc cataaaggga cgctgttgga
    17281 aacctatttt gggcagtatc ttggtcaatg gctcacacat aatgggttat tggacaaggc
    17341 aagctgtgaa agtgggaagc agattgaggt ttatgctaat agcctgcaac gtacaattgg
    17401 cactgcacaa gctttggttg ccggtgggtt ttcaggttgt aatgtacaag ttaaacacca
    17461 ccagcagatt ggaaagatgg atccggtgtt taatccaatt attcgctccg atgaccagac
    17521 atttgtgcaa acagcattaa gcaaaattga cctcaataaa acccatcagt atttagcgcc
    17581 atcctatcat ttattaagcg aaataatcga ttatgctcat tcagaaaaat gtttgcagga
    17641 aaaacagtgt gaatttttta atgatgttgg taaattaagg attaaaaagg ataaagaacc
    17701 aggtgtttcc ggtacgatgc gattaagtga aaatattgtc gatgccttat tgctgcaata
    17761 ttatgaaggt cttcctttaa aagagattgc aaataaccag cttgatagtg atcagaaatg
    17821 gcaaacactc aataaaatta aaaacgatta ccaatttatg ctacgaggag atccatttat
    17881 tgctaaacat atttcattac cgctattaaa atatattcag caagatttaa atagtgaaaa
    17941 taaaataaca cttttagtcg gacacgattc caatattata gccttacttt ctgcattaaa
    18001 tgttaagcct tataaattag cacatcaata tgaacaaact tcaataggag ggaaagtctt
    18061 tattgaaatc tgaaaagaaa aaagtagcca agcacaaaaa gttaaactgg aatatgtata
    18121 tcaatctact gaccaaattc gtaaagcgat tccactaagc cttgataaac caccgcaaca
    18181 tcacattttg agtttggatt attgccaagc tgaccaatat ggattatgcg attatatgga
    18241 atttaaacag ttactggaaa aagtcattgc tgatacattg tgagattttg acaaagtgag
    18301 cgaaaaaggt agcataaaaa agtcgggaca gttgccagct gctccctgta agatgttgtt
    18361 tacagcacta gtaatgctac tatccccaaa aagctgctga aaggcagctt ttttgttgta
    18421 tataaaagta cacctttcta cgcttcattc tttttctcca attaaaccta atttaggact
    18481 tctagtgata aattcggtat catatcttaa ttcactacat atcgtgcaag ccttaaaatt
    18541 gaaagggctg acgaaatcct atttaattct gacacacata agggaatatt atgtcgcaag
    18601 atattcaagc cgatttaaaa aagatattgg cactattaag ccgtaaccgt cgatttcaat
    18661 cattattttt attactgtta agaagtgaac aatttattaa tagtgatgca ctttctacgg
    18721 agttacagct ttcagtacga acggtaaaaa aagagattaa gttattaaaa gaaaaacttg
    18781 aaccttacgg cattcagatt ttttctcgac cgttttatgg ctataaatta ttgattcagt
    18841 ttgaagaatt acacacgcaa ctaaaacagt atttccaaat atcacagccg atgacggtaa
    18901 ataataaatt tgagtctcgg gtaaatgcga ttttacgccg attactggtg agccagcgtg
    18961 cattgaaagt tgaagatttt cagagtgagc tttatttaag ttttaccagt gccttaacaa
    19021 aagaatttag tcaagttaaa gccttgttag gaa
//
LOCUS       DANJWC010000028        18981 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.028, whole genome shotgun sequence.
ACCESSION   DANJWC010000028 DANJWC010000000
VERSION     DANJWC010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18981
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.028"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..1932
                     /locus_tag="PWX02_000740"
     CDS             <1..1932
                     /locus_tag="PWX02_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDL1728774.1"
                     /translation="NDTAEISIFDEIGFWGVTAQQFAKDLKALGNNLKQINLHIHSPG
                     GDVFDGIAIYNLLKNHPANKTVYIDGLAASMASVIAMAGSEIIMPENAMMMIHKPWGI
                     QGGDADDMRKYADLLDKVESTLVTAYSSKTGKSTDELAEMLKEETWLNARECIELGFA
                     DKLANPLVAMASIQSKKLEDYTKMPNEIKAMLFTPQGNAGAATPTPEQPQPPAQAVEN
                     PQNVTKPDAMAVLAQRNAAIKATFAAFGTQFDGLLAECLADVSMTAEQAKDKLLAKLG
                     ENTTPSVPQNHIHAGNGNIVGDSVKQSLMARAGQDTDKTNARDNAYNAMTLRELARAS
                     LVDRGVGISGYTPMQMVGLAFTHSSSDFGQILIDVAHKSLIKGWETATENYEQFTTRG
                     ILTDFRPAKRVGLGEFGYLPEVGEGEEYTYGTIGDEGAQVALATYGQLFSITRQAIIN
                     DDMHLLTKIPEKMGQAAKATIAKLVFALITGNAVAQDGKKLFDSSHKNTLANAKLDVE
                     HIDKAIQLMNGFVNSRGEPLSIEPEFMLLPTSLHTKAKQILGSSSVEGADINSGIINP
                     IRDIVSPIKSPRLQIADPKSWYLLNKEAIEVSYLDGVDSPYIEQQHGFTVDGVSTKVR
                     IDAGVNVIDYRGVVKVTNA"
     gene            2011..2172
                     /locus_tag="PWX02_000741"
     CDS             2011..2172
                     /locus_tag="PWX02_000741"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728775.1"
                     /translation="MQYNIAREVRKMLELMNKSTKANVLIFGIWAMGLLFALSAFISA
                     MTNFISILH"
     gene            2185..2508
                     /locus_tag="PWX02_000742"
     CDS             2185..2508
                     /locus_tag="PWX02_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HDL1728776.1"
                     /translation="MAKNYIQDGNTVRLTTTKAVTSGDVIVTEDLIAIAVTDAAKNEA
                     VVGLTTGVFSVKAKQADDIKQGAVLYWSEADGATITAGSNKRLGIAWKASGTSSATVD
                     VKINV"
     gene            2501..2803
                     /locus_tag="PWX02_000743"
     CDS             2501..2803
                     /locus_tag="PWX02_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL1728777.1"
                     /translation="MSSPFEQAMAAADQTIQHTMMSEWLIGGKPYPATYDEAPAIFNG
                     LHSSDDRAIHGTERTLTLFRASGYKPRLDDRVSGNGKKYLVKSYHFVDQLIVLQLE"
     gene            2807..3331
                     /locus_tag="PWX02_000744"
     CDS             2807..3331
                     /locus_tag="PWX02_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL1728778.1"
                     /translation="MGSKVTGLKELQASLKKIARQTVPKAAAQAIRTVGRQAMNKAVK
                     SVAAEIGVNQKTIKGRAKMTAKPTPSRLQATIKVNRTHMPMIRILERKSNRLSVSKGS
                     IRVGKHTVQRGFRQRLANGRTHIMFRQGRKRYGIDVAKVPLSQPLTQAFEQELKKYPE
                     QVQAELAKQLAGKL"
     gene            3328..3720
                     /locus_tag="PWX02_000745"
     CDS             3328..3720
                     /locus_tag="PWX02_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL1728779.1"
                     /translation="MKIHTKIRKEVLELLQTKLPDIENFYNGLPTFIDIEEQQLAVSV
                     SLDDISLQEMTVCNDQWSAKLNITIHLKSVENAEDELDEWAEKIREIIDYSDFEYLET
                     ISLSQYQYEQDQNQRTWYTATLIFDVEY"
     gene            3748..4389
                     /locus_tag="PWX02_000746"
     CDS             3748..4389
                     /locus_tag="PWX02_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728780.1"
                     /translation="MAKTTKVQGTKFRISVGREAQKAITAINLNTATLTIASSGYKKG
                     DAIEITGCGQLDGIYPVLSVTGEQVKLCEEVKWEGKDLPTSYTKAKAALVQFSEQFCA
                     VKNIEKSDDTLNTEDVTTVCSEGTETEPGEIEFGSIKLSFFHKPTTEMQARLRELFFN
                     KSTFAYKLELPDNHGTTYGVGFIESGNGFSGEVKGKYEGSVSIKPSKRDYLLV"
     gene            4472..4873
                     /locus_tag="PWX02_000747"
     CDS             4472..4873
                     /locus_tag="PWX02_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005714571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728781.1"
                     /translation="MTLREKLLANKPKLQPIEINGETYYLREATVGDMNKQIFETRSW
                     LIQQAEQENVELPAEDDETFDEALNRFGEKYRLAQSVAYRLCDENGALLFNPLNIDDL
                     NAIAELDSKVIIDFNQAVSAPKDSASEESSS"
     gene            4918..5148
                     /locus_tag="PWX02_000748"
     CDS             4918..5148
                     /locus_tag="PWX02_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HDL1728782.1"
                     /translation="MPERHFAEYQLFYQEQPFGLWREDYRTAQLAHLTAMINRDPKGK
                     APELAEFMPFFNRSEDVEEMEDNGVADYLAKR"
     gene            5211..5438
                     /locus_tag="PWX02_000749"
     CDS             5211..5438
                     /locus_tag="PWX02_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728783.1"
                     /translation="MFEALDRFMINTIKIAIIVFLVACIFISMPNWWIASILVLFPTA
                     YLLDKYWWRNTEMYRRKMDKLVKEMKNNRQI"
     gene            5492..9043
                     /locus_tag="PWX02_000750"
     CDS             5492..9043
                     /locus_tag="PWX02_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL1728784.1"
                     /translation="MSSSNRLTITLDVENSKLLAGFAQSDTVANKFARSFILNMDQAQ
                     QKARQFADRSTKYLKNIEQAANNLNSSVSKERFWQIANRLQFPTSKVISYANSYTELG
                     NKLKLVTESEAQQARAMAAVYDISLKTAQSTQATSSVYQTFAQNAKQLGISQNDVAKL
                     TETVAKSVAISGASSATASNALVQFSQSLLMGKLKAQEFNSLMTQTPSVIQAIAKGLG
                     ITTAELKAMVDNGEMSAEKMIEGLKKAESYVNGQYAKTTTTISGAMQNLSTAAEKWVG
                     EMDSTVGASQKAVAVLNALNAHFDTFAKTALVAGGAFVLFKGYMSVRPFIEARAAIYE
                     NRKAIIAENQARQQTIATRQQELVVLSQQTALKLEQTLQTYNQNKAELEAIAVKQQKI
                     TKEREEIAVAIQAAATRTERLALSAELQALDQKEIALGQQKIAVERQQIATKQALKVA
                     YAENAVAQSAMNAGMVTATTTTRAMNGVLAAARAELNLLKAAALSNPLMTLAMVATTA
                     GTALWGFFESQKAAREEALRYADSLDSVRANIEKMTKAQTDAEMVKLSRSIKEQETAL
                     ASLKQRQAELTREIEQGATVLDDAFGAGVRFEKSAKELAKAQEELKLVTADVEKAQNT
                     LNASLEAQEALQAHVPIANLREEFVKLYPHIDESQIKVDGLNIAIGNFTVKSPEMVIA
                     ANNIANALGGVAGEAMRAAILVANLSSMKLDANGAAIIDPKHLEKIEQIERNNAIASA
                     KGKDKIALQVKDALIKSGMKEGDAGYERLKAAYEQQFTLQNAPKRGGKPKKTKAERSA
                     ESYQNQVAEMTNRLSGLKIDKEDLVKYGGISQYQEVRKLTEDIALNAEKYKNYGTEGV
                     AKLKELAAKIDSEQQLLDIEQFQVDHDKKLEAMQFELDLLGKTRTEKELLQFNNQLDL
                     EAERLKINMTKENIALLDQKIAKIKEEYELHQKKQEETRASVMMGIKEGWNNIETDVS
                     NVAANVANITQNTFDGMADSLTNFVMTGKADFRSFANSILSDLSKMIIKMMLFNAIKQ
                     GATMLFGYQGPTTTVNGNAVYGLGTWATGGYTGNGGKYTPAGIVHKGEYVITKEATSR
                     LGLDYLNYLNYGRRGFASGGGVAVPRVPSSSYQPKSAQSSISVQVINNGEPVDAKVSQ
                     KQQGEQTQITVELMRKIARQEANGMIQNNFRAGGVFA"
     gene            9043..9372
                     /locus_tag="PWX02_000751"
     CDS             9043..9372
                     /locus_tag="PWX02_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL1728785.1"
                     /translation="METLQWCVRTDLAVENTPEIMEVKFGDGYTQRSPKGLNNLLRAY
                     TGTIKVKKGEHLAVEAFFAKHRGVSPFYFKDPYTQQNKKVVCSSWPAKMVGLNHWEFS
                     VTFKEVP"
     gene            9372..10088
                     /locus_tag="PWX02_000752"
     CDS             9372..10088
                     /locus_tag="PWX02_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL1728786.1"
                     /translation="MPVSISNQMKLDLAKLEQNAMLDLYEVDLRTLKDKSGNAGSVYR
                     FYSGLNELKTSIVWQGRTYDPYPIEATGFERNSSGPSNRPTLTLSNLFGLVTGIANQF
                     DECIGAIVRRHQVYAQYLDAVNFAGGNAKADPNQEIISHFVIEQLTSLTRETATFTLA
                     LPIETDNAKIPSRIIMADTCTWIYRSAECGYTGKQYFDEKDKATSDPKADKCSHCLNG
                     CELRGNQRNFGGFVSVNKLG"
     gene            10092..10823
                     /locus_tag="PWX02_000753"
     CDS             10092..10823
                     /locus_tag="PWX02_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL1728787.1"
                     /translation="MELEQQIIDYALQHEPHEMCGFVVFDGNKNRFIPCENQAEDKAN
                     YFEISDLDYIKAEEKGELVAVVHSHPEPNGKPILSVLDRKMQVQTGLDWWLVHNRQIH
                     KFRNVPHLIGREFKHGTMDCYTLYRDAYMLAGYEMDEFERQDDWWHTGQNLYLDNIQG
                     QGFERVETPQIGDVILMQVGADVPNHAAIYIGEQMVIHHSPNRLSKRDLYDGYWLRHT
                     HSIWRHKLADKLDFDGILNDIAVNN"
     gene            11092..11682
                     /locus_tag="PWX02_000754"
     CDS             11092..11682
                     /locus_tag="PWX02_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDL1728788.1"
                     /translation="MITVKFYGHLKTFGTDFKILVKDIAEAIRALCSQLKGLREALRD
                     GVYKVRIGKQYLDPSALEKGLFYCLKKGQTIHFTPVIKGAKSGGVFQAVLGVALIGAA
                     FFLGPVGWGVLGASSAMMMGAMGASMLLGGVAQMLTKMPNAPNIGNEKEKENSTAFSN
                     LNNLVAQGKPVPLAYGLIRTGSLVISQGVETMTVEV"
     gene            11685..18818
                     /locus_tag="PWX02_000755"
     CDS             11685..18818
                     /locus_tag="PWX02_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HDL1728789.1"
                     /translation="MGLFSRKRKNHTPKEAPETGRSKQIVNIVELLCEGEIEGLVDGF
                     KSIYLDGTQIQNDDGSYNFNNVSGQLNVGTQDQNVLDGYDSSQNEVSVGVEVKKKSGA
                     IVRTVTDERISRLRLTLGVRSLFHQNNQGDTNTTNVDLKITIGTRQYSHSFNGKYSSQ
                     YLESVVFDNLPPVPFNISVERVTKDSNSQRLQNGTIWSSYTEIIDTEFTYPNSAVAGI
                     SFDSEYFNNIPTRNYLIKAKKVKVPSNYDPVKRTYTGFWDGTFKVAWTNNPAWEIYDL
                     APILSKMLGVEISFDKWALYDVARYCDQLVPDGMGGMEPRFTCNVWLTEVKTAYDLLN
                     DFCSVFRAIPIWTGTEVSVIIDRPRDPVWTYTNANVVGGFERSYSARKSRHNAVQVTY
                     SDKTNGYESAIEYVSDDEEIKKHGLNLSQITAFGCTSRGQAHRTGKWILETEKREKET
                     ITFTVGREGLMHLPGDIIRVADSHYAGTEIGGRVLAINGRKVTLDREISIDNASYFTY
                     INGEATHSSIKIQSVNGKEITLDSTPTGLETYGVWSLSTKQISSGLYRSISIVENADG
                     TNTITALQHEPQKEAIVDNAAHFVETARTLYKAPQINAVEVSTGYDGKLYISSDISSG
                     DGKLTYDIKINKDGNLYQFKKGLADPNIELSDLPNGDYSVIIYGKNAKGQIVTEKTQT
                     FTIDRPPAPTGVVVTGGLGQITLEWDWVNEVTQTEIFAAETDNFALAKKIAKVTARTY
                     AHTLKGNKVVRYYWLRHTRGINVGPFYQQQGVRGQTAVDLDARLTELNTQLSRNIVNE
                     VFDVAAPARGLELVKTVANLTDKGTKLASSQVYNQADGKLYTWNGTAYSATVAAEDVT
                     GKLSKSKIDTSLISQLTGADNTANLARRLAETAQSNINQEITNRQDAVTAEANNRTKA
                     IQAESANLTKKIQAEATARGAAVTQLQNVDAQQAQLISAVTAKANNALSGLEEEKTAR
                     ANGDKAEAQAREALTARMGAAESNIVTIQRTVANNAQSISEVSQNLNAKIDNINIGGR
                     NLLRDSEFNAYNKWGSPQIEFAENANRRTIKVTSTGTNGPVGICSSNRHSTSYFQQGE
                     TYTLSLFARGSKALDYLYLMRQDGNNVRLPVINVASETEFNYYKLTFKAPFTTQQGYF
                     LVGFFQSTPNQFVEFHSLKLEKGNVATDWTPAPEDVDSAVSAVSADLTSYKQTQAATD
                     SAQAQQLNQLSVNLTKAETNFNAKITEEKKARVDADKANAERLTDITSRVANAESTIT
                     NFQSTKANKSEVASIAQQNLQSIWRTDAQSAVDALKIGGANLLVDSEYLTTARWGGSS
                     RVASSQYGDRRLTQVFVTQAGTGHFGVTQGTQKATTRIRQGETYTLSLNAQGTAGFTR
                     TGLNYVYLIREDGGNFRLPTLPLTASLSQRPKVTFTAPWTSNQVRLLIGANGIFEATD
                     WFAFHSVKLEMGNVATGWTPTAKDIDDKVSAVQSNLTAYQAAQAKADQAKATQISGLT
                     TRMGAAESNLTRTERAVTELNQTTVTTLRDLTARTKTTEGSLSRLETAKANKTEVASI
                     AQSSLQSIWKADAKSAVDSLSIGARNLLIDSTYNQHINYNTKMSSITRTVYRGNMLIR
                     LGMLGGAGVAGIVQAPAAQVSNIRQGQEYTLSLNVQGTAGIQKTGLNYVFLMRADGAN
                     QRLATIPVIASLLNRPKITFTAEWTSERAYLLVGVNGTYETTDWLAFHSVKLEKGNVA
                     TDWTPAPEDVDSAVSAVSAKIDTIQETLANADSALSSRIDTVTASVGSNLAKITQVSN
                     AVAGVDGKLSSTHTIKTETIAGGRKAIAGIALGAAADNRNAESSVIVMADKFEVVKNA
                     QDGQPVRLFGVAQNKVAINGDLIATGTIKGSHIDADSVRAGILTAGAIRTEHLAAGQI
                     SADKLAIGLGGNLLVNPIFATPDLSIAPFGWNYWRGEVGDRLNIDTRNIFNRNKQDNY
                     GLKNGGLPNELVFSMRYSTTKETSDTGRIGWLSQDINVVPNKKYIASVWLACHRGQAK
                     LVIENIEKPGGAYLGRIGNSEVITGYSAHQGEFKNMKRVKVVFTAPTSGCIRFALRLD
                     NIQGKANPFLFARRPMLEEASDNVTDITQPSPWQNAGVTAIHGGSIVTNTVTAQQIAA
                     NTITANEIAAGTIAARNMAANSINASHIVGSSITADKLNVNNLAAISANLGKVTAGTI
                     TGTTISGGTISGTTITGGIIKGARIEGLTGKFTGELEINQLIGGNIYEAKTWTSKATG
                     KSKTYSETKNSFALSDTYYEYVCQFVLPATKATRTLYVVHTPTGESKSKTFYGSGSNR
                     TPTTITLPSVIDISNTISPNVSNPFNDSGAILIANTQYVVTCRAWCAGRGRSAKVNFR
                     AIISSTNGSSYQL"
BASE COUNT         6031 a         3914 c         4325 g         4711 t
ORIGIN      
        1 aatgacaccg ccgaaatctc aatttttgac gaaatcggct tttggggtgt caccgcccaa
       61 caatttgcta aagacttaaa agcccttggc aacaatttaa aacaaatcaa tctgcatatc
      121 cactcgcccg gtggtgatgt gtttgacggc attgccattt acaacctgct aaaaaatcac
      181 cctgctaaca agaccgtcta tattgacggt cttgctgcat ctatggctag cgtaattgca
      241 atggcaggca gcgaaatcat tatgccagaa aacgctatga tgatgatcca caaaccgtgg
      301 ggcattcaag gcggcgatgc cgatgatatg cggaaatatg cggatttgct tgataaagtg
      361 gaaagcacgc tagttacggc gtacagctcg aaaactggta aatctaccga tgaattagca
      421 gaaatgctga aagaagagac ttggcttaat gcccgtgagt gcattgagtt gggttttgct
      481 gataaactcg ccaatcccct tgtggcgatg gcgtctattc aatctaaaaa attagaggac
      541 tatacaaaaa tgccaaacga aatcaaagca atgttattta cgccacaagg caatgcgggg
      601 gctgcaaccc caactcctga acaaccccaa cccccagcgc aagcggtcga aaatccacaa
      661 aatgtgacaa aacccgatgc gatggcggta ttagctcaac gtaatgcggc gatcaaagcg
      721 acctttgccg ctttcggcac acaatttgac ggcttgctgg cagaatgttt agctgatgtg
      781 tcaatgaccg ctgagcaagc caaagacaag ctccttgcca aactgggcga aaacaccacc
      841 ccaagcgtac cgcaaaacca cattcacgca ggtaacggta acatcgttgg cgacagcgtg
      901 aagcagtcgt taatggctcg tgctggtcaa gataccgaca aaaccaacgc aagagataat
      961 gcctacaatg caatgacctt gcgtgaattg gctcgtgcct cattggtcga tcgtggcgtg
     1021 gggattagcg gttatacgcc aatgcagatg gtcggtcttg cctttaccca ttcgagttcg
     1081 gactttgggc aaattctgat tgatgtggcg cacaaatcct tgattaaggg ctgggaaacg
     1141 gcgacagaga attacgagca gtttaccact cgtggcattc tgactgactt ccgaccggca
     1201 aaacgggttg gattgggtga gtttggttac ctgccggaag tgggcgaagg ggaagaatat
     1261 acctacggca ccatcggcga tgaaggggcg caagtggcat tggcgaccta cgggcaatta
     1321 tttagcatta cccgtcaggc gatcatcaat gacgatatgc accttttaac caaaatcccg
     1381 gaaaaaatgg ggcaagcggc aaaagccact atcgccaaat tggtgtttgc cttaatcacc
     1441 ggcaacgctg tcgcccaaga cggaaaaaaa ctgtttgata gcagccataa aaatacgctt
     1501 gcgaatgcga agttagatgt tgagcatatc gacaaggcaa tccagttaat gaatggcttt
     1561 gtaaacagcc gtggtgaacc gctctctatt gagccagaat ttatgctgtt accaacctca
     1621 ttgcatacca aagcaaaaca gattcttggt tcgtcaagcg tggaaggtgc agacatcaac
     1681 agcggtatca ttaacccgat ccgtgatatt gtcagcccga ttaaatcgcc tcgcttacaa
     1741 attgcagatc cgaaatcatg gtacttgctc aataaagagg cgattgaagt ctcttatctg
     1801 gacggggtgg atagccctta catcgagcaa cagcacggct ttacggtgga cggtgtttcg
     1861 accaaagtcc gtattgatgc cggtgtcaat gtgattgact accgtggcgt ggtgaaagtg
     1921 accaacgctt aattgcaaaa taataaaccc cgagttagcg gaataactcg gggttttgtt
     1981 ttaaccctta tcagtcaagg attaaattaa atgcagtata acatagcaag agaggtacgc
     2041 aaaatgttag aactaatgaa taaatctacc aaagccaatg tgttgatttt cggtatttgg
     2101 gcgatggggc tactttttgc tttatcggcg tttattagtg caatgaccaa ttttatttct
     2161 attcttcatt aaaggaacct aattatggct aaaaactata ttcaagacgg caatacggtg
     2221 cgtttaacca ccaccaaagc cgtaacctcc ggtgatgtga ttgtcaccga agatttaatt
     2281 gcgattgcag taactgatgc ggcaaaaaac gaggcggtag tcggtttaac caccggtgta
     2341 tttagtgtga aagcgaagca agcagacgac atcaaacaag gtgcggtgct gtattggtcg
     2401 gaagcagacg gggcaacgat cacggctggc tcaaacaaac gcttaggcat tgcctggaaa
     2461 gcctcaggca catcatctgc aacagttgat gtgaaaatca atgtctagcc cgtttgagca
     2521 ggcaatggca gccgccgatc aaaccatcca acacacaatg atgagcgaat ggttgattgg
     2581 cggtaagccc taccctgcga catacgatga ggcaccagcg atttttaatg gcttgcactc
     2641 aagcgatgac cgagctatcc acggcacgga acgcacgcta accctatttc gggcgagtgg
     2701 ctacaaaccg agattggacg accgagtgag cggaaatggc aagaaatacc ttgttaaatc
     2761 ctatcacttt gtcgatcagt taatcgtatt gcaattggag taaacaatgg gttctaaagt
     2821 gaccggttta aaagaactgc aagcctccct gaaaaaaata gctaggcaaa ccgtacctaa
     2881 agcagccgct caagcaatcc gtactgttgg gcggcaagca atgaataagg cggttaaatc
     2941 ggtggcggca gagattggcg taaatcaaaa gacaatcaaa ggcagagcaa aaatgacggc
     3001 aaaacccaca ccgtcccgac tgcaagccac gattaaagtc aatcgtaccc atatgccgat
     3061 gatccgcatt ttagagcgaa aatcgaatcg cttgtcagta agcaaaggca gtattcgggt
     3121 aggaaaacat acggtgcaac ggggctttcg ccaacggctt gcgaacggga gaacgcacat
     3181 tatgtttcgt caaggtcgta aacgctacgg cattgatgta gcaaaagttc cgctatccca
     3241 accacttacg caagcatttg agcaagagtt gaaaaaatac ccagaacaag tgcaagctga
     3301 attagccaag caactcgcgg ggaaattatg aaaatccaca ctaaaatccg aaaagaagtc
     3361 cttgaattat tacagaccaa attaccggat attgagaatt tttataacgg gctgccaact
     3421 tttattgata ttgaggagca gcaacttgca gtgtcggttt ctctcgatga catcagtctg
     3481 caagaaatga ctgtttgtaa tgatcaatgg agtgccaagc tcaacattac aatccatttg
     3541 aaatctgtcg agaatgcaga agatgaatta gatgagtggg cggaaaaaat tcgcgaaatt
     3601 atcgactatt ccgactttga gtatttagag accatctcac tctctcaata tcagtatgaa
     3661 caagaccaaa accaaagaac atggtacaca gcgacgttaa tctttgatgt tgaatattaa
     3721 acaaacaatt tcaataggag aattacaatg gcaaaaacga caaaagtcca aggtacgaaa
     3781 tttagaatta gcgttggacg tgaggctcaa aaagcaatta ccgcaattaa cttaaatact
     3841 gctactctaa cgattgcatc atcaggctac aaaaaaggcg atgccatcga aattaccggt
     3901 tgcggtcagt tagacgggat ttatcctgtg ctgtccgtga caggcgaaca agttaaactt
     3961 tgcgaagaag taaaatggga aggtaaagac ttaccgacta gttatactaa agcgaaagct
     4021 gctttggtac agttctccga gcagttctgt gcggttaaaa atatcgaaaa atccgatgac
     4081 accttaaata cagaagatgt tacaacggtt tgctcggaag gcacagaaac tgagccgggt
     4141 gaaattgagt ttggctcaat taaattgagc tttttccaca agccaactac agaaatgcaa
     4201 gcccgcttac gcgaactgtt tttcaacaaa tccacctttg cctataaact ggaattaccg
     4261 gataaccacg gcacaactta cggtgtaggc tttatcgaat ccggcaatgg ttttagcggt
     4321 gaagtcaaag gcaaatacga aggctctgta tctattaagc caagcaaacg tgactacttg
     4381 ttagtttaat tgcaagaaat caaaaaattc gaccgcttgt agaaataaca agcggtcttt
     4441 ttatatcaga attttacaaa aggaacgaac aatgacactg cgtgagaaac tccttgccaa
     4501 caaacctaaa ttacaaccta tcgaaatcaa cggtgagacc tactacctac gtgaagccac
     4561 tgttggcgat atgaataagc agatttttga aacccgaagc tggctcattc aacaagcaga
     4621 acaagaaaat gttgaattac cggcagaaga tgatgaaacc tttgatgagg ctctcaaccg
     4681 ttttggcgaa aaataccgcc ttgctcaatc ggttgcctac cgcttatgtg acgaaaacgg
     4741 tgcattactg tttaacccac ttaacattga cgatctcaat gcgattgccg aattagacag
     4801 taaagtgatt atcgacttta accaagccgt gtccgcccca aaagactcag cgagcgaaga
     4861 aagttccagc taaccctttc gctcgcactg agcaaaaccc ttgaagaaat cgaacaaatg
     4921 cccgaacgcc actttgccga atatcaactt ttctaccaag aacagccttt cggcttgtgg
     4981 cgggaagatt accgcaccgc ccaacttgcc cacttaaccg caatgatcaa ccgtgatccg
     5041 aaaggcaaag cccctgaact tgcagaattt atgccgtttt ttaatcggag tgaggatgtg
     5101 gaggaaatgg aagataatgg tgtggcagat tatttggcga aaaggtaaag aaaacagctt
     5161 gaaattcctt gctcttggat atatatttcc tcaaagagaa ggagggaagt atgtttgaag
     5221 cactagaccg ttttatgatt aatacgatta aaattgcaat tattgtattt ttggtcgctt
     5281 gcatctttat tagtatgccg aactggtgga tagcttctat cttagtactt ttcccgactg
     5341 cctatttgct tgataaatac tggtggcgaa ataccgaaat gtatcgccgc aaaatggata
     5401 aattagttaa agagatgaaa aataatcgtc aaatctaaat taacatcaat tctagcccgc
     5461 tttatgcggg ctttttattt ggggtgaaaa agtgagtagc tcaaacagat taactattac
     5521 cttagatgta gaaaattcta agttactagc tggattcgca caatctgata cagtagcaaa
     5581 taagtttgct cgtagcttca ttttgaatat ggatcaggct cagcaaaaag cacgtcaatt
     5641 tgctgatagg tcgacaaaat atctaaaaaa tattgaacaa gcagcaaata atcttaatag
     5701 ctctgtatcc aaagaacgtt tttggcaaat tgcaaatcgg ttgcaattcc cgacaagtaa
     5761 agtgattagt tatgccaata gctacaccga actcggtaat aaactcaaat tagtcaccga
     5821 aagtgaagcc caacaagccc gagcaatggc ggcggtgtat gatatttcgc tcaaaaccgc
     5881 ccaatctaca caggcaacct cttcggttta tcagaccttt gctcaaaatg caaaacagct
     5941 cggtatttct caaaacgatg tggcaaaact aacggaaacg gtagccaaat cggttgctat
     6001 ttccggtgca agttcggcaa ccgcttcaaa cgccctagtg caattcagcc aatcgttgtt
     6061 aatgggtaag ctcaaagccc aagaatttaa ctcattgatg acccaaacac cgtctgttat
     6121 tcaggcgatt gccaaagggt taggcattac cactgccgaa cttaaagcaa tggtggataa
     6181 cggtgagatg tctgctgaaa aaatgattga ggggctaaaa aaagcagaga gttatgtaaa
     6241 cggacagtac gccaaaacaa ccactacaat tagtggagca atgcaaaatc tttcaactgc
     6301 ggctgaaaag tgggttggcg aaatggatag taccgttggt gcgtcacaga aagccgttgc
     6361 cgttctgaat gcgttaaatg ctcattttga tacgttcgct aaaacagcac ttgtcgcagg
     6421 tggtgcattt gtgcttttca aaggctatat gagtgtacgc ccatttatcg aggctagagc
     6481 ggcgatttat gagaatcgaa aagcaattat tgcggaaaat caggctcgcc aacaaacgat
     6541 agctactcgt cagcaggaat tggttgtact ctctcaacaa accgccttaa aactggaaca
     6601 aacattacaa acctacaatc aaaataaagc cgaattagag gcgattgcgg ttaaacagca
     6661 gaaaatcaca aaagaacgag aagaaattgc tgttgctatt caagcggcgg caactcgtac
     6721 ggaaagatta gcgttatctg ctgaactgca agcgctagat cagaaagaaa tcgctctcgg
     6781 gcaacagaaa atagcggttg aacgccagca aattgcaaca aaacaggctc tcaaagtggc
     6841 gtatgctgaa aatgcagttg ctcaaagtgc aatgaatgca ggtatggtca ctgcaaccac
     6901 aacaaccaga gcgatgaatg gggttttagc ggctgcaaga gctgaattga acttactgaa
     6961 agcagctgca ctgtctaacc cattaatgac tcttgcaatg gtggcaacga ctgccggtac
     7021 tgccctttgg ggattttttg aaagtcaaaa agcagctcgt gaagaagccc tccgctacgc
     7081 ggacagctta gactccgttc gagccaatat cgaaaaaatg actaaggctc aaactgatgc
     7141 agaaatggtt aaattgtctc gttctatcaa agaacaggaa acagcattag ctagtctgaa
     7201 acaacgccaa gctgaactta cacgagaaat cgagcaagga gctacggttt tagatgatgc
     7261 ctttggtgcc ggtgtccgtt tcgaaaaaag tgcgaaagag ttagcgaaag cacaagaaga
     7321 gttaaagtta gttactgctg atgttgaaaa agcacaaaat acattaaatg ccagtttaga
     7381 agctcaagaa gccttacaag ctcacgttcc aatcgctaac ctacgagagg aatttgtcaa
     7441 attatatcct cacattgacg aaagccaaat caaagttgat ggcttaaata ttgctatcgg
     7501 taattttacc gtcaaatcac cggagatggt tattgccgct aataacattg ctaatgctct
     7561 tggtggcgtt gccggagagg ctatgagagc ggctattcta gtcgctaatc tgtcaagtat
     7621 gaagctggat gctaacggtg ctgcaattat cgatcctaaa catcttgaaa aaatcgagca
     7681 aattgaacgt aacaatgcga ttgcttcggc taaaggcaaa gataaaatcg cattgcaagt
     7741 aaaagacgct ctgattaaat caggtatgaa agagggcgat gccggatacg agcgactaaa
     7801 agcagcatat gagcaacaat ttacgttgca aaatgcccct aagcgcggcg gaaaaccgaa
     7861 gaaaaccaaa gccgaacgct ctgccgaaag ttaccaaaac caagtagcgg agatgaccaa
     7921 tcgtttatcc ggcttaaaga tagataaaga agatctcgta aaatatggtg gcatttctca
     7981 atatcaggaa gtgcgtaaac ttactgaaga tattgctctg aatgctgaaa aatataaaaa
     8041 ctacggcact gaaggcgtgg ctaaactcaa agagctggct gctaaaattg attctgaaca
     8101 acagctttta gacattgagc aattccaagt cgatcacgat aaaaagctag aggcaatgca
     8161 attcgagctg gatttactgg gaaaaactcg tactgaaaaa gaattgttac aatttaacaa
     8221 tcaattagat ttagaggccg agcggctgaa aattaatatg acgaaagaga atattgctct
     8281 tttagatcag aaaattgcca aaattaagga agaatacgag ctacatcaga aaaagcagga
     8341 agaaactcgt gcttctgtaa tgatgggaat aaaagaaggc tggaacaata ttgaaaccga
     8401 tgtcagcaat gtggcggcaa atgttgccaa tattactcaa aatacctttg acggtatggc
     8461 ggatagcttg actaattttg tgatgacggg taaagcggat tttcgtagtt ttgctaattc
     8521 catattatct gatcttagca aaatgattat caaaatgatg ttatttaatg ctatcaaaca
     8581 aggtgcaact atgttgtttg gctaccaagg accgactaca acggtaaatg gtaatgctgt
     8641 ttatggatta ggtacttggg caacaggtgg ctacacaggc aatggcggca aatacacccc
     8701 agcgggtatc gtccacaaag gggaatacgt catcaccaaa gaggcaacct ctcgtcttgg
     8761 tttggattat ttgaattatc ttaactatgg cagacgtggt tttgcctctg gcggtggtgt
     8821 cgctgtacca agagtaccgt catcatctta tcagccgaaa tcggctcaaa gtagtatcag
     8881 cgtacaggtc atcaataatg gtgagccggt agatgccaaa gtcagccaaa aacagcaagg
     8941 cgagcaaacg caaattactg ttgagttaat gcgtaaaatt gcccgtcagg aagcgaacgg
     9001 aatgattcag aacaacttta gagccggagg agtatttgcc taatggaaac attacaatgg
     9061 tgtgttcgca ccgatttagc ggttgaaaat acccctgaaa taatggaagt caaattcggc
     9121 gatggttata cccaacgctc gcccaaaggc ttaaacaatt tattacgagc ctatacaggc
     9181 acaatcaaag tgaaaaaggg ggaacattta gcggtggagg cattttttgc caaacatcgt
     9241 ggtgtttccc ctttttactt taaagatccg tacacacaac aaaacaaaaa ggtagtgtgt
     9301 agcagttggc ccgcaaaaat ggttggctta aaccattggg aatttagtgt aacctttaag
     9361 gaagtaccct aatgcctgtt agcatatcca atcaaatgaa actcgacctt gccaaacttg
     9421 agcaaaatgc aatgcttgat ttgtacgagg tagatttacg cacattaaag gataaaagcg
     9481 gtaatgctgg ttcagtttac cgcttttatt ctgggctaaa tgaactcaaa acaagtattg
     9541 tctggcaagg tcgaacttac gacccttacc ccatcgaggc aacaggattt gagcgtaaca
     9601 gcagcggccc aagcaatcgc cctactctta cgctatcaaa tctgtttggc ttagttacgg
     9661 gtattgctaa tcagtttgac gaatgtatcg gtgcgattgt ccgccggcat caggtttatg
     9721 ctcaatattt agatgccgtg aattttgccg ggggtaatgc gaaagctgat ccaaatcagg
     9781 aaataatcag tcattttgtg attgagcagc ttaccagcct cacacgagag actgctactt
     9841 ttacgcttgc gttgccgatt gaaaccgata atgccaaaat ccccagcaga attattatgg
     9901 cggacacctg cacttggatt taccgctcgg cggaatgtgg ctacaccggc aagcagtatt
     9961 ttgatgaaaa agacaaggca acatcagacc caaaagccga taaatgcagc cattgcctga
    10021 acggttgtga attgcgaggc aatcagcgta attttggtgg atttgtatcg gtgaataaat
    10081 tggggtaatg aatggaattg gaacaacaaa taatcgacta cgccctccaa cacgaaccgc
    10141 acgaaatgtg cggctttgtt gtttttgacg gcaataaaaa tcgatttatc ccttgcgaaa
    10201 accaagcgga agacaaagcc aattactttg aaatctccga tcttgattac atcaaagcag
    10261 aagaaaaagg cgaactggta gcggtggtac attcacatcc cgagccaaac ggcaagccta
    10321 tattatctgt gcttgatcgc aaaatgcagg tacagaccgg tttagattgg tggctagtgc
    10381 ataatcggca aatccataaa ttccgcaatg taccacattt aatcgggcgt gagtttaaac
    10441 acggcacaat ggattgttac acgctctacc gtgatgccta tatgcttgcc ggttacgaga
    10501 tggacgagtt cgagcgacaa gatgactggt ggcataccgg gcaaaatctt tatttggata
    10561 atatccaagg gcaaggtttt gagcgagtgg aaaccccaca aatcggcgat gtgattttaa
    10621 tgcaagtagg tgctgatgtg ccgaaccacg ctgccattta cattggcgaa cagatggtaa
    10681 tccatcatag cccgaaccga ttatctaagc gtgatttata cgacggctat tggttacgtc
    10741 atacccacag catttggcgg cataaactgg cggataagtt agattttgac ggcatattga
    10801 atgatattgc tgtaaataac taacaaaaaa tgaccgcttg taacaaaaac aaaccccgaa
    10861 gcgtttgcag cacttcgggg tttttcatat ccacttacct aactaagagg acataaattg
    10921 aatgaatgat aaacgattta cattcaaatt tctaggagtt cttatggaag caataaatat
    10981 taccccaaaa gaaatcagaa aaacaatgtg gacagccgca ataattctct ttttgtttgc
    11041 gttcatctgg aaagcccctg aatttatcac tgctattcgt tggtggtaac tatgattaca
    11101 gttaaatttt acggacacct taaaacattc ggcacagatt ttaaaatttt agttaaagac
    11161 atcgctgagg caattcgtgc tctgtgttca cagcttaaag gattgcgtga agcgttgcga
    11221 gacggtgttt ataaagtccg tatcggtaag cagtatttag acccatcagc ccttgaaaaa
    11281 gggctttttt attgcctgaa gaaagggcaa accattcatt ttacccctgt tatcaaaggg
    11341 gcgaaaagcg ggggtgtttt ccaagctgtg ttaggtgtag ccttaattgg agccgcattt
    11401 tttctcgggc cggtcggttg gggagttcta ggggcttctt ccgcaatgat gatgggagca
    11461 atgggggcat caatgttgct tggcggtgtt gctcaaatgc ttaccaaaat gccgaatgcc
    11521 ccgaatatcg gtaatgaaaa agagaaagaa aactcaacgg ctttttcaaa tttaaataac
    11581 ttggtggctc aaggcaaacc tgtgccgttg gcttatggct taattcggac cggctcgctt
    11641 gtgatatcgc agggagttga gacgatgact gtagaggtat aattatggga ttatttagtc
    11701 gtaagagaaa aaatcatact cctaaagaag caccggaaac cggtcgctct aaacagattg
    11761 taaatattgt ggaattgctg tgcgagggcg aaatcgaggg cttggttgat ggctttaaat
    11821 cgatttactt agacggcacg cagatccaaa acgatgacgg cagctataac tttaataatg
    11881 tgagtggtca gttaaatgtc ggtacgcaag accaaaatgt gttagacggt tacgacagct
    11941 cacaaaatga agtgagtgtg ggtgtagaag tcaagaaaaa aagcggtgca atcgtccgca
    12001 ccgttactga tgaacgcatc agccgtttgc gtttaacgtt gggcgtgcgg tcactattcc
    12061 atcaaaataa tcaaggcgat accaatacca ccaatgttga tttaaaaatc acgattggca
    12121 cacggcaata ttcgcatagc tttaacggca aatacagctc gcaatacctt gaatctgtgg
    12181 tgtttgataa cctgcctcct gtaccgttta atatctcggt tgaacgtgta acaaaggata
    12241 gcaattctca acgactacaa aacggtacaa tttggagcag ctataccgaa attatcgaca
    12301 ccgaatttac ttaccctaat tcagcggttg ccggtatcag ttttgattcg gagtatttta
    12361 ataatatccc gacccgaaac tacctaatta aagcgaaaaa agtcaaagtg ccaagcaatt
    12421 atgatccagt taaacgcact tacaccggct tttgggacgg cacatttaaa gttgcgtgga
    12481 cgaacaaccc tgcttgggaa atttacgatt tagctccgat tttaagcaaa atgcttgggg
    12541 ttgaaatcag ttttgacaaa tgggcgttgt atgatgttgc tcgctattgc gatcaacttg
    12601 tgccggacgg tatgggtgga atggagcctc gtttcacctg taacgtatgg ctcacggaag
    12661 ttaaaactgc ctatgacttg ttgaatgatt tctgctcggt attccgtgcg attccgattt
    12721 ggaccggaac agaagtatcg gtcattatcg accgtccgcg tgatccggtt tggacttaca
    12781 caaatgctaa tgtagttggt ggatttgaac gatcctactc tgctcgcaaa tcacgccata
    12841 atgcagtgca agttacttat tcggataaaa caaatggcta tgaaagtgcg attgaatatg
    12901 tctctgatga cgaggaaatc aaaaagcacg gcttaaattt aagtcaaatt actgctttcg
    12961 gttgtacctc tcgggggcaa gcacaccgta ccggaaaatg gattttggaa accgaaaaac
    13021 gggaaaagga aaccattact tttaccgtgg gacgtgaggg cttaatgcac ttgccgggtg
    13081 atattatccg tgttgccgac agccattatg ccggtacgga aattggcgga cgtgtactgg
    13141 cgattaatgg cagaaaagtt acccttgacc gtgaaattag cattgataac gccagttatt
    13201 ttacctatat caacggtgag gcaacgcact ctagcattaa aatccaatcg gtaaacggta
    13261 aagaaatcac gctagatagc acgccaaccg gcttagaaac ctacggcgta tggtcgttat
    13321 cgactaaaca aatcagctcg ggcttatacc gctccatctc gattgttgaa aatgcggacg
    13381 gtacgaatac gattacggca ttgcaacacg aaccgcagaa agaggcgatt gtagataacg
    13441 ctgctcactt tgtggaaacg gcgagaacgc tttacaaagc cccgcaaatt aacgcagtag
    13501 aagtctcgac tggttatgac ggcaagctct atatctccag tgatattagt tctggcgacg
    13561 gtaagctaac ctatgatatt aaaatcaaca aagatggtaa tttgtatcag ttcaagaaag
    13621 gattagccga cccaaatatt gagttgagcg atttaccgaa tggcgattac agcgtgatta
    13681 tttacggcaa aaacgccaaa gggcaaatcg tcaccgaaaa aacacaaacc tttactattg
    13741 atcgacctcc agctccgacc ggtgtagtag taacaggtgg attagggcaa attaccctcg
    13801 aatgggattg ggtaaatgaa gtcacacaaa ccgaaatttt tgcggccgaa accgataatt
    13861 tcgcccttgc aaaaaaaatc gcaaaagtga ccgctagaac ctacgctcac acactaaaag
    13921 gtaacaaggt tgtacgctat tattggctac gccataccag aggcattaat gtcggaccgt
    13981 tttatcaaca acagggggta agaggacaaa cagcggttga tttggatgct cgattaaccg
    14041 aattaaacac tcagttatca cgcaatattg ttaatgaggt ttttgatgtt gctgcaccgg
    14101 caagaggttt agaattagtg aaaacagtcg ctaatttgac cgacaaaggt actaaactgg
    14161 cgtcatctca agtttacaat caggctgacg gcaagctcta tacttggaac ggtacagcat
    14221 actcagctac agtggctgcg gaagatgtta ccggcaaatt atcaaaatct aaaattgata
    14281 cgtcccttat ttcgcaatta acaggtgcag ataacaccgc aaatttggca agaagattag
    14341 cggaaacagc tcaaagcaac attaaccaag agattactaa ccgacaagat gccgttactg
    14401 ccgaagcaaa taaccgtact aaagccattc aagccgaaag tgcgaattta acgaaaaaaa
    14461 ttcaagctga agcaaccgct cgtggggctg ctgttacaca gttacaaaat gttgatgctc
    14521 aacaagcaca actcatttct gctgtcacag caaaagctaa taatgctcta tcaggcttgg
    14581 aagaggaaaa aacagcacga gcaaacggcg ataaagctga agctcaagca cgtgaagcat
    14641 taacagcaag aatgggagca gctgaaagca atatcgtaac tattcaacgt acggtagcaa
    14701 ataatgccca aagcattagt gaagtcagtc agaatttaaa tgctaagatt gataatatta
    14761 atatcggcgg tcggaattta ttaagagaca gcgagttcaa tgcgtacaat aaatggggta
    14821 gcccacaaat tgaatttgct gaaaatgcaa atcgccgaac tatcaaagta acctcaacag
    14881 gtactaacgg tcctgttggt atttgttcat ctaatcgcca ttccacatct tatttccaac
    14941 aaggagaaac ttatacactc tcattatttg ctcgaggaag taaggcacta gactatctct
    15001 atctaatgcg tcaagatggg aataacgtta gattaccagt gattaacgtt gcctctgaaa
    15061 cagagttcaa ttattacaaa ctgacgttca aagcaccgtt taccacacaa caaggctatt
    15121 tcttagttgg ttttttccaa tctactccaa atcaatttgt tgagtttcat agtttaaagc
    15181 tagaaaaagg aaacgtagcg acagattgga caccagcacc ggaagatgtg gacagtgcgg
    15241 ttagtgctgt ttcggctgat ttaacgagtt ataaacaaac tcaagcggct accgattcag
    15301 cacaagcaca gcaacttaat cagttatctg tgaacttgac caaagcagag actaatttca
    15361 acgccaaaat cacggaagaa aaaaaagctc gtgttgatgc agataaagcg aatgcagaga
    15421 ggctcacaga cataacaagc cgtgttgcaa atgcggaatc aactattacc aatttccaat
    15481 caaccaaagc gaataaaagt gaggtggcaa gtattgccca gcagaactta cagagcattt
    15541 ggcgcaccga tgcgcaaagt gcggtggatg ccttgaaaat cggtggtgca aatctactgg
    15601 ttgatagtga gtatttgacg acagcccgtt ggggcggtag ctcacgagtg gcaagctcgc
    15661 aatatggtga tcgtcgcttg actcaagtgt ttgtgaccca agcaggcaca gggcattttg
    15721 gtgtgactca aggcacacaa aaagccacta cgcgtattcg tcagggtgaa acctatacct
    15781 tgtcactaaa tgcgcagggg acggcaggtt ttacccgcac agggttaaac tatgtgtacc
    15841 ttatccgcga agatggtggc aattttagat tgccaacttt gccgcttact gcatcattgt
    15901 cccaacgccc taaagtgaca tttactgcgc cgtggacgag caatcaagtt cggttactga
    15961 ttggcgcaaa tggcatattt gaggcgacag actggttcgc atttcacagc gttaagctcg
    16021 aaatgggtaa tgttgccaca ggctggacac caaccgccaa ggatattgac gacaaagtat
    16081 cagcggttca gtccaacctc accgcttatc aggcggcaca agctaaagca gatcaagcca
    16141 aagctacgca gatttcgggg ctcacaaccc gaatgggggc agcggaaagt aacctcactc
    16201 gcaccgaacg ggcagtgacg gaacttaatc agacaacggt gacgacactg cgtgatctaa
    16261 ccgcgcgcac caaaacaacg gaaggttcgc tctctcgttt ggaaaccgcc aaagcaaata
    16321 aaactgaggt ggcaagtatt gctcaaagct ccttacagtc aatttggaaa gctgatgcaa
    16381 aatctgccgt tgattcactt tctatcggtg caaggaactt actgatagac agtacttaca
    16441 accaacacat caactacaat acaaaaatgt catcaatcac acgcacagtc tatcgcggca
    16501 atatgttgat acgactcgga atgcttggtg gcgcaggtgt ggccggtatt gtacaagcac
    16561 cagccgctca agtaagtaat atcagacagg gtcaagagta cacacttagc ctgaatgtac
    16621 agggtacagc cggcatacaa aaaacggggc tcaactatgt attcttgatg cgtgctgatg
    16681 gggctaatca acggttagca acaatacctg ttatagccag cctattaaac agacctaaaa
    16741 taacctttac tgccgaatgg acaagtgagc gagcctatct gcttgttggt gtaaatggta
    16801 cttatgagac cacagattgg ttagcgtttc acagcgtcaa acttgaaaaa ggcaacgtag
    16861 ccacagactg gacgccagca cctgaagatg tggacagtgc ggttagtgca gtttcggcta
    16921 aaattgatac tattcaggag actttagcga atgccgactc tgcattaagc tctcgtattg
    16981 ataccgttac tgcttctgtt ggctcaaact tggcaaaaat tactcaagtg agtaatgctg
    17041 tagccggtgt agatggtaaa ttatcgtcta ctcataccat caaaaccgaa accattgccg
    17101 gtggacgtaa agcgattgcc ggtatcgcac ttggtgccgc ggcagacaat cgcaatgcag
    17161 aatcctctgt cattgtaatg gcagacaaat ttgaggtagt aaaaaatgct caagacggcc
    17221 aaccggtacg attgtttggt gtggctcaaa ataaagttgc gattaatggg gatttaattg
    17281 caacaggtac aattaaaggt agccacattg atgcagactc agtaagagcc ggaatattga
    17341 cggcgggagc tatccgtacg gaacacttgg cagcagggca aatatcggcg gataagttag
    17401 cgattgggct aggcgggaat ttgctggtta atcctatttt tgcaacaccc gatttgtcaa
    17461 tagctccgtt tggctggaac tactggcgag gagaagttgg tgatcggcta aatattgaca
    17521 cccgtaatat tttcaatagg aataaacaag ataattacgg actaaaaaat ggaggactac
    17581 caaatgagtt agtattttcg atgcggtata gcactacaaa ggaaacctct gatactgggc
    17641 gaattgggtg gttatctcag gatattaatg ttgtgcctaa taaaaaatat attgcctccg
    17701 tatggttagc ttgtcatcgt gggcaagcta aattagtcat tgagaatatc gaaaaacctg
    17761 gtggtgctta cttaggtcgg ataggcaaca gcgaggtaat tactggctat agtgctcatc
    17821 aaggcgaatt taaaaatatg aaacgggtta aggttgtatt tactgcacct acttcaggct
    17881 gtattcgttt cgcattacga ttagataata ttcaaggtaa agctaatcca tttttatttg
    17941 ctcgtagacc aatgcttgag gaggctagtg ataatgttac agatatcact cagcctagcc
    18001 cttggcaaaa tgccggtgta accgctatcc acggtggctc aatagttact aataccgtta
    18061 ctgctcaaca aattgcagca aataccatta ccgccaacga aatcgcagcc ggcacgattg
    18121 cagctcgaaa tatggcagca aactcgatta atgcaagtca tattgtgggg agttcgatca
    18181 ctgcggataa gctaaatgta aacaatttag cggcaatatc ggcaaatctt ggtaaggtaa
    18241 ctgctggtac aattacaggt acgacaatta gtggtgggac aatttcaggt acaacaatta
    18301 cagggggaat aattaaagga gcaagaattg agggattaac aggtaaattt actggagaac
    18361 ttgaaatcaa ccaattgatc ggaggtaata tctatgaggc taaaacttgg acatcaaaag
    18421 caacagggaa aagtaaaaca tattcagaaa cgaaaaacag ttttgcatta agtgatacat
    18481 attatgagta tgtatgtcaa tttgtactac ctgctacaaa agcgacgaga acattgtatg
    18541 ttgtccacac tccgacagga gagagtaaaa gcaaaacttt ttatggtagt ggtagtaatc
    18601 ggacaccgac aacaattaca ttaccgtctg taattgatat tagcaatact atttcaccta
    18661 atgtatctaa cccatttaat gattctgggg caatactcat tgctaatact caatatgtag
    18721 taacgtgtcg tgcgtggtgt gcagggcgtg ggagaagtgc taaagtgaat tttcgagcaa
    18781 ttatatcatc tacaaatggt tcatcttatc aattatagga ggcaaatatg aaaaaaatta
    18841 tactacttac aacaattatg gtttcagctt gtacaactca atataagcaa gactatacaa
    18901 caaaaccaac cttttgttat caactagccc ctcaagagca attaccgggg aaaaactgta
    18961 ttggctcagg agggcattca g
//
LOCUS       DANJWC010000029        18906 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.029, whole genome shotgun sequence.
ACCESSION   DANJWC010000029 DANJWC010000000
VERSION     DANJWC010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18906
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.029"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(4..1206)
                     /gene="coaBC"
                     /locus_tag="PWX02_000756"
     CDS             complement(4..1206)
                     /gene="coaBC"
                     /locus_tag="PWX02_000756"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDL1728790.1"
                     /translation="MLQNKKILVGISGGIAAYKTIEFIRHLKKANAEVRVVLTPAAEA
                     FVTPLTLQAISGNAVSTSLLDPQAELAMGHIELAKWADLIVIAPASADFIARLRMGMG
                     NDLLSTLCLATASPILLAPAMNQQMYKQIAVQENLSVLVNRGVQLIGPNSGFQACGDV
                     GAGRMSEPEEIYQAVLAHFSNNQDLEGLNVTITAGPTREAIDPVRYISNHSSGKMGYA
                     IANAFAERGAKVTLISGPVNLPTPKNVERINVESAVEMEQQAVKFAQKSAIFIGCAAV
                     ADYRMAAVSDQKIKKTDANDELTLKLVKNPDIIANVANLAENRPFVVGFAAETQNVAE
                     YAKSKLERKNLDMICANDVSGGQVFGQDQNALHLFWKNGEKTLPLADKSKLAESLVQE
                     IVKHYREK"
     gene            1356..2093
                     /gene="radC"
                     /locus_tag="PWX02_000757"
     CDS             1356..2093
                     /gene="radC"
                     /locus_tag="PWX02_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDL1728791.1"
                     /translation="MEEKMNDFFDEDSQEPTMMPREKLLTQGAASLTDAELLAIFLRT
                     GTKAVPVLALAESVLMAFGSLRQLLNANIDDFCKIYGLGKTKYIQLQASKEMTKRYLA
                     QQMEFSEMIQAPYMAIMYFQTELEEEEREVFMVLFLDNQNRLIYKEKMFFGTINQTAV
                     HPREIIKRALKYNAAAIIVAHNHPSGSCLPSESDRSLTKKIEMACELVDIRFVDHIIV
                     GKGDYFSFEEEKLEMKEHSFLQISDRK"
     gene            2324..2560
                     /gene="rpmB"
                     /locus_tag="PWX02_000758"
     CDS             2324..2560
                     /gene="rpmB"
                     /locus_tag="PWX02_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDL1728792.1"
                     /translation="MSRVCQVTGKRPAVGNNRSHAMNATKRRFLPNLHTHRFWVESEN
                     RFVTLRLTAKGMRIIDKKGIDAVLAEIRARGEKI"
     gene            2572..2742
                     /gene="rpmG"
                     /locus_tag="PWX02_000759"
     CDS             2572..2742
                     /gene="rpmG"
                     /locus_tag="PWX02_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL1728793.1"
                     /translation="MAAKGAREKIRLVSTAETGHFYTTDKNKRNMPEKMEIKKFDPVV
                     RKHVIYKEAKIK"
     gene            complement(2823..3578)
                     /locus_tag="PWX02_000760"
     CDS             complement(2823..3578)
                     /locus_tag="PWX02_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL1728794.1"
                     /translation="MSKNRLEQILLFLKNHNIATVEQLVKVTEASPATIRRDLIKLDE
                     EGVVIRTHGGVSLNQFIPFQPTTNEKQFKQIVEKERIAEYAVSLISPGNSILLDAGTT
                     TLCIAKKIAHMPLRVITTDLHIALLLSEYKQIELIITGGRVDNSSQSCIGNHSLTLLE
                     NINPDIAFVSCNSWSLEKGVTAPTEEKANLKRMLQQNAKQKVLVADSSKYGKYSLFRA
                     TQLDNFTQIITDKGLPIEYSQALSSNDFKLSLV"
     gene            complement(3686..4892)
                     /locus_tag="PWX02_000761"
                     /pseudo
     CDS             complement(3686..4892)
                     /locus_tag="PWX02_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248712.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
     gene            complement(4889..5887)
                     /locus_tag="PWX02_000762"
     CDS             complement(4889..5887)
                     /locus_tag="PWX02_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDL1728795.1"
                     /translation="MSTQTSKSIVPIYDGMNKIPGGAMVIPLILGSIIGTFFPEFLNL
                     GSFTTALFKSSAGPLIALLIFATGMQITVRSSGPVLAHTGVVLLFKTIIPALLVVILG
                     LWVGNDGVMGVSLLAMLTVVANSNGGIWLAFTGKYGDYRDKGAYVASAVNDGPFFVLL
                     FLGVSGLADIPLSLMLAAVIPLIIGMVIGNLDPKWTELMKPTGAIVIPFFAFALGTGI
                     NLQSIITGGATGIILGIVASLFTGFLVFIGYKTILRRGNQSGIGFAAGTTAGNAIITP
                     EIVAQADPTLAPFVQTATAQIAAAVLVSAILAPLFAAWVLKRQGGLEQDTIKGENL"
     gene            complement(5898..6899)
                     /gene="pdxA"
                     /locus_tag="PWX02_000763"
     CDS             complement(5898..6899)
                     /gene="pdxA"
                     /locus_tag="PWX02_000763"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HDL1728796.1"
                     /translation="MKPILGITMGDAAGIGPEVIIKALANQRIYELAHPVVIGDRKII
                     QRALPIVHSELKLRILNNLDNFVAEYGVIDILDLNNLPEDLPFAQVDPRAGKAAYEYI
                     EEAVNLAMGGKIHAIVTAPLNKEALHAGGKPFPGHTEILATLSNTKDYSMMLTSEKLN
                     VIHVTTHVQLRKACDLITKERVATVIDLAEENARILGFKEPRIAVAGLNPHSGENGMF
                     GDEDQKEIVPAIQEAQAKGFNVTGPVPPDTLFHRAANLNEFDIVVVMYHDQGHIPIKL
                     LGFDTGVNVTVGLPFIRTSVDHGTAFPIAGKGTADSRSMTEALYLGAKMANIKYATH"
     gene            7333..8697
                     /locus_tag="PWX02_000764"
     CDS             7333..8697
                     /locus_tag="PWX02_000764"
                     /EC_number="2.6.1.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005820549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HDL1728797.1"
                     /translation="MTIKTPIQAVLASNQYFLDRQDAMESNVRSYPRKLPFAYQKAQG
                     VWVTDVEGNEYLDFLAGAGTLALGHNHPKLIQAVKDVLDSGLPLHSLDITTPLKDAFT
                     EELLSFFPKDKYILQFTGPSGADANEAAIKLAKTYTGRGNVIAFSGGFHGMTHGALSL
                     TGNLSAKNAVQGLMPGVQFMPYPHEYRCPFGIGGEAGAKAVEHYFENFIEDVESGVVK
                     PAAVILEAIQGEGGVVPAPISFLQKVREVTQKHGILMIVDEVQAGFCRSGKMFAFEHA
                     GIEPDIVVMSKAIGGSLPLAVLAIKKEFDAWQPAGHTGTFRGNQLAMATGYVSLKIMC
                     EENLAKNAQERGEYLTNALRELSKELPCIGNVRGKGLMMGIDIVDERQPKDATGAYPR
                     DCDLAVAIQKYCFKNKLLLERGGRGGNVVRVLCAVNITQAECEEFIKRFKQSVAEAVK
                     AVRG"
     gene            8787..10322
                     /gene="ddc"
                     /locus_tag="PWX02_000765"
     CDS             8787..10322
                     /gene="ddc"
                     /locus_tag="PWX02_000765"
                     /EC_number="4.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,4-diaminobutyrate decarboxylase"
                     /protein_id="HDL1728798.1"
                     /translation="MSNLEKHRQSLFCSDPKSIADYESAMNNAVKAVSDWLKEEKMYT
                     GGSIKELRAKINGFNPTQAGVGVQKSQEHLVEIFLKPSLKVHHPHSLAHLHCPTMVTS
                     QIAEVLINATNQSMDSWDQSPAGSIMEEHLIDWLRQKVGYGKGTSGVFTSGGTQSNLM
                     GVLLARDWAIANHWKKADGSEWSVQADGIPADAMQKVKVVCSENAHFSVQKNMAMMGM
                     GFQSVVTVPSNANAQMDVEALAKTLADLKAEGKIVACIVATAGTTDAGAIDDLKAIRK
                     LADEHQAWVHVDAAWGGALLLSKDFRHFLDGIELTDSITLDFHKHFFQSISCGAFLLK
                     DEANYRFIDYKADYLNSEYDEEHGVPNLVSKSLQTTRRFDALKLWFTVEALGEELYAS
                     MIDHGVYLSKEVEAYINATDGLEMLVPAQFASVLFRVVPQGYPAEFVDTLNQNVADEL
                     FARGEANIGVTKVGEHQSLKMTTLSPIATLENVRALLAQVLAEADRIKDSIVNGTYVP
                     PID"
     gene            10425..10793
                     /locus_tag="PWX02_000766"
     CDS             10425..10793
                     /locus_tag="PWX02_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NirD/YgiW/YdeI family stress tolerance protein"
                     /protein_id="HDL1728799.1"
                     /translation="MKKVLGLTLMAMALSACNSTASNGSSQANTGVAPANVISVKQAL
                     AAADDSYVTVEGTLLNKVGDETYLFKDATGQIRVEIDDEVWAGQHVGSKGNKVRLYGE
                     IDKGFNKTELDVKTLTVIKP"
     gene            complement(10868..11455)
                     /locus_tag="PWX02_000767"
     CDS             complement(10868..11455)
                     /locus_tag="PWX02_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL1728800.1"
                     /translation="MNHLIIYAHPNPSSFNHAILEKTIAASVPNKVVVRDLYQLNFKP
                     ILDWAEFQGTLSKHYEADVAIEHQYWLEADVITLIYPLWWMGFPAILKGYLDRVLSYD
                     FAYRNGEIESIGLLTGKKMQQFVTLGNSNEKYERKGFLAAFEHTLGKGLFEFCGIRDV
                     KMHYFGNIGLKETDYTAILQQITASCSTILKEQAV"
     gene            11631..12179
                     /gene="napF"
                     /locus_tag="PWX02_000768"
     CDS             11631..12179
                     /gene="napF"
                     /locus_tag="PWX02_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HDL1728801.1"
                     /translation="MTDYPNQSLPRRSFLRGQFLDSLKSQKVSQQGYEPIRPPWANLA
                     NFLEKCTACGNCIQACETQIIVKGAAGYPEIDFSRGECTFCEKCVHSCEAEVFRAVSE
                     QPWSHKIEIQAGCLLKLGTECRSCGDSCEMRVIRFRPSLGGIAEMVLNLESCNGCGAC
                     LSVCPTSAIKIQPLQTQANRDE"
     gene            12172..12438
                     /locus_tag="PWX02_000769"
     CDS             12172..12438
                     /locus_tag="PWX02_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HDL1728802.1"
                     /translation="MSEKLSEQENWYVCSLVVQAKPEKLEEVKAEILKIPHTEIHGEK
                     TDEGKLVVTIESNVHLALSDRIDQVRDIKGVIVVSLISNYIDEQ"
     gene            12477..14975
                     /gene="napA"
                     /locus_tag="PWX02_000770"
     CDS             12477..14975
                     /gene="napA"
                     /locus_tag="PWX02_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005566990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HDL1728803.1"
                     /translation="MELNRRDFMKANAAVAAAAAAGIMIPVKNVQADDTGIRWDKAPC
                     RYCGTGCSVLVGTKDGKVVATQGDPDAEVNRGLNCIKGYFLSKMMYGADRMQEPMLRM
                     KDGKFDKNGDFTPVSWDQAFSIMAEKIKTTIKNDGPNAVGMFSSGQTTIFEGYAKVKL
                     WKGGFRSNTIDPNARHCMASAAVAFMRTFGMDEPMGCYNDIEKTDAFVLWGSNMAEMH
                     PILWSRISDRRLSDNKVKVVVMSTYEHRSFELADTPIIFKPHSDLAILNYIANYIIQN
                     DKVNWDFVNKHTKFKRGETNIGYGLRPEHKLQQNTNAKTAGKMYDSDFEEFKKIVAPY
                     TLEEVHRISGVPKDQLETLAKLYADPNQKLVSFWTMGFNQHTRGVWVNHMMYNVHLLT
                     GKIALEGCGPFSLTGQPSACGTAREVGTFIHRLPADMVVTNPKHREIVEKSWKLPAGA
                     IPDVPGFHATAQSRALKDGKMKFLWQMCTNNMQGGPNINEEIFPGWRNPETFIVVSDP
                     YPSVSAVAADLILPTCMWVEKEGAYGNAERRTQFWRQQVKGPANARSDTWQLVEFSKY
                     FKVEEVWPEELLAKAPELRGKTLYEILYRNGKVDSYQVPTNIPGYMNDEAEHFGYYIQ
                     KGLFEEYAEFGRGHGHDLADFDTYHKVRGLRWPVVKDEKTGEYKETLWRYREGYDPYV
                     KAGEEVAFYGNADKKAVILGVPYEPPAEAPDEEYDLWFCTGRVLEHWHTGTMTRRVPE
                     LHKAFPTNLVWMHPNDAKKRGLRHGDKVKVISRRGEYVSNLDTRGRNKCPEGLIYSTF
                     FDAGQLINKVTLDATDPISFETDFKKCAVKVVKA"
     gene            15064..15969
                     /gene="napG"
                     /locus_tag="PWX02_000771"
     CDS             15064..15969
                     /gene="napG"
                     /locus_tag="PWX02_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HDL1728804.1"
                     /translation="MKMDPNRRQFLKNATRTAAGVCGVGIILGLQQQQSFARQGVALR
                     PPGALPEQDFLSACTRCGQCVQACPYDMLHLASLISPVEAGTPYFIARDKPCEMCVDI
                     PCMNACPTGALSEELKDIDDARMGLAVLLDHETCLNWQGLRCDVCYRVCPLIDKAITL
                     EKVHNDRTSIHAKLIPTVHSDACTGCGKCEQACVLEEAAIKVLPMDLAKGLLGRHYRL
                     GWQEKQNAGKSLIEDVHPEGLRPAYDARMPEGLHEPVYQHMEVKPDVKVATPSRATQN
                     YVPNSTTVEAPEHFPDLDLNLKGVK"
     gene            15969..16847
                     /gene="napH"
                     /locus_tag="PWX02_000772"
     CDS             15969..16847
                     /gene="napH"
                     /locus_tag="PWX02_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HDL1728805.1"
                     /translation="MAANSPKNAGLEARQKLGWWHAYRFLILRRLSQLSIILMFLSGP
                     LWNVWILKGNYSGSLLLDTVPMTDPLMTAEVLATGYRPEWTVLVGALIMVAFYGIVGS
                     KLFCSWVCPMNMVTDTAAWLRRKLGIRQTAKIPRNLRYVILAVILVGSAISGTLLWEW
                     INPVAALGRVFVFGLGAMLWLVLVVFLFDLLVAEHGWCGHLCPIGATYALIGAKSLIK
                     VKVVDRNRCDNCMDCYNVCPEPQVLRVPLHGKPEESTIILSKDCITCGRCIDVCAENV
                     FAFGSRFQGSIDVKNI"
     gene            16893..17321
                     /locus_tag="PWX02_000773"
     CDS             16893..17321
                     /locus_tag="PWX02_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HDL1728806.1"
                     /translation="MRKYLALILAVMSGFAMAEAPKLSGTIDETTESVAPGFHNPQKD
                     VGTIPTTFPFQPPLVPHSIRGLQVSKNVNHCLGCHAIEPSKTTGATRLPPSHFTDRDG
                     KVYENESPRRYFCLQCHVQQTDVNPIIQNKFESVRESQAK"
     gene            17332..17940
                     /locus_tag="PWX02_000774"
     CDS             17332..17940
                     /locus_tag="PWX02_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDL1728807.1"
                     /translation="MLRRFCNWLRSPSKMAIGAVILISALGGIFAWSGFNAGLAMTNT
                     EEFCSDCHMNDVVPEYRASAHYSNRSGVKAICSDCHVPHEFFPKWQRKIIAAKEVYAH
                     FIGKVDTKEKFEAHRGEMAEREWARMKANGSQECRNCHNFDDMDFTQQKNIAQQMHAL
                     AQEQGKTCIDCHKGIAHNLPHMEAIQKNFIPDDLLKKAEEKK"
     gene            complement(18002..18844)
                     /gene="cpdA"
                     /locus_tag="PWX02_000775"
     CDS             complement(18002..18844)
                     /gene="cpdA"
                     /locus_tag="PWX02_000775"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HDL1728808.1"
                     /translation="MDAKRMHEYIKLPVTGVIKLLQITDPHLFSNPEETLLNVKTVKS
                     FSAVIEQINKQAKQYFDLVLATGDLIQDHNIAGYHYFAQITNSLNSPIVWLEGNHDVQ
                     PSMSEILAQYKHILPHKQILLGEQWQLLMLNTQVVGMPYGELSYSQLKWLEEKLAQYP
                     QRFTLVALHHNILPTNSAWLDQHSLKNSNELSEIFAKFKNVKAIVHGHIHQQVDDIWH
                     GIPIYATPSTCIQFKPNCDQFTLDLQPQGWREIELHENGTIETVVKRLNSNDFLPNVN
                     AIGY"
BASE COUNT         5610 a         3719 c         4096 g         5481 t
ORIGIN      
        1 tcattatttt tctcgataat gttttacaat ttcctgtacc aaactttctg ccaatttgct
       61 tttatccgcc agaggaagcg ttttttcacc atttttccag aacaaatgca acgcattttg
      121 atcctgcccg aacacttgtc cgcctgagac atcattggca caaatcatat ccagattttt
      181 gcgttcgagt ttcgatttag cgtactccgc cacattttgc gtttctgcgg caaaaccaac
      241 cacaaaaggg cgattttcgg caagattggc gacgttagcg ataatatcgg ggttttttac
      301 caattttagc gtgagttcat cattggcatc ggttttctta attttttggt cagagactgc
      361 cgccattcgg taatcggcaa ccgctgcaca gccaataaaa atcgcagatt tttgggcaaa
      421 tttgaccgct tgttgctcca tctcaaccgc agattccaca ttaattcgtt ccacattttt
      481 cggcgttggt aaattcaccg gaccgctaat caacgtaacc tttgctccac gttcggcaaa
      541 ggcattagca atcgcgtagc ccattttgcc ggagctgtgg ttgctgatat aacgcacagg
      601 gtcaattgcc tcacgagtcg gtccggcggt aatggttaca tttaagccct ctaaatcttg
      661 attgttggag aaatgagcca acaccgcttg gtaaatttct tcaggctcag acattctgcc
      721 agcccccaca tcaccacagg cttggaagcc actattcggg ccgatgagct gaacgccacg
      781 attgactaaa acagacagat tttcttgcac cgcaatctgt ttgtacattt gttgattcat
      841 tgccggtgca agcaaaatcg ggcttgcagt cgcaaggcag agagtggaaa gtaaatcgtt
      901 gcccattccc atacgcaggc gagcaataaa atcggcactt gccggggcga taacgattaa
      961 atccgcccat tttgccagct caatatgccc catcgccagt tcggcttgtg ggtcgagtag
     1021 tgaagtggaa accgcattgc cggaaatcgc ttgtaaggtc aaaggcgtta caaaggcttc
     1081 tgctgctggg gttagcacta cacgcacttc cgcatttgcc ttttttaagt ggcggataaa
     1141 ttcaatggtt ttgtaggctg caatcccacc ggaaatgccg actaaaattt ttttgttttg
     1201 taacatcttt actatcctta aagcgtggat ttattttgca tattttagcg gaataaggag
     1261 ttgctcacaa cttcactatt tttgcgatct tgctcgaaaa attgaataaa tccctttgtc
     1321 tgcaaaggtt ggctctttta tgcttaacta aattgatgga ggaaaaaatg aacgattttt
     1381 ttgatgaaga ttctcaagag ccaacaatga tgccgcgtga gaaattatta acacaggggg
     1441 cagcctcatt aaccgatgcc gaattattag cgatattttt acggactggt acgaaagctg
     1501 tgcctgtgct ggcattggca gagagtgtgt tgatggcatt tggctcgtta cgccaattac
     1561 tgaatgcaaa tattgatgat ttttgcaaaa tttacggttt aggcaaaacc aaatacattc
     1621 agttacaagc ctctaaagag atgaccaaac gctatctggc tcaacaaatg gaatttagcg
     1681 agatgattca agccccttat atggcgatta tgtatttcca aaccgagttg gaggaagaag
     1741 aacgggaagt ttttatggtg ctgtttttgg acaaccaaaa ccgattgatt tataaagaaa
     1801 aaatgttctt cggcaccatt aatcaaactg ctgttcaccc ccgagaaatt attaaaagag
     1861 cgttaaaata taatgccgcg gcgattattg tggctcataa tcacccctca ggttcttgtc
     1921 tgccgagtga gtcggacaga agtttaacta aaaagatcga aatggcgtgc gaattggttg
     1981 atattcgctt tgtggatcat attattgtcg gcaaagggga ctatttttcg ttcgaggaag
     2041 agaaattaga gatgaaggag cattcatttt tgcaaatttc tgatagaaaa tgaccgcttg
     2101 ctttatgtaa cctttaacat ttctcctgaa acagtaaaag atacgcatat aaattctact
     2161 tgaatataac aaatgaaatc ggtataatcg gcagtcttta ttacatagac tgggtcgggt
     2221 taatgcctga cgaggcaatc aaaatcggag gggttggttg agcaacgcct tttgtgagat
     2281 ctcccgaacc atttttaagc ttaaaattat tggagaaatt caaatgtcaa gagtttgcca
     2341 agtaaccggc aagcgtccag cagttggtaa caaccgctca cacgcaatga atgcgactaa
     2401 acgtcgtttc ttaccaaacc ttcacactca ccgtttctgg gttgagagcg aaaaccgttt
     2461 cgtaacctta cgtttaactg cgaaaggtat gcgtattatt gataaaaaag gcattgatgc
     2521 agtattagct gaaatccgtg ctcgtggcga aaaaatctaa ggagctaaaa aatggcagct
     2581 aaaggcgctc gtgagaaaat ccgtttagtt tctacagcag aaactggtca cttctacaca
     2641 acagataaaa acaaacgtaa tatgcctgaa aaaatggaaa tcaaaaaatt tgatccagtt
     2701 gtgcgtaaac acgttatcta caaagaagca aaaattaaat aattttgttt taagatttaa
     2761 aacccagcga atgctgggtt tttcatatac aaaaaattag tgaaaaaaga ccgcttgtat
     2821 gattaaacta atgatagctt aaaatcatta cttgacaagg cttggctata ttcaataggt
     2881 agtcctttat ctgtgataat ttgtgtaaag ttatcgagtt gagttgcacg aaataaagag
     2941 tattttccat atttgctgct atcggcaacc agtacttttt gtttggcgtt ttgttggagc
     3001 attcttttta aattagcttt ctcttctgta ggtgctgtaa ctcctttttc taagctccaa
     3061 gaattacagc tcacaaaagc aatatcagga ttaatatttt ctaataaagt taaactatga
     3121 ttaccaatac aagattgact actattatct actcgtcccc ctgtaataat taattctatt
     3181 tgcttatatt cagagagtaa taaagcaata tgtagatcag ttgtaatcac tcgaagtggc
     3241 atatgagcga tttttttcgc aatgcatagc gttgtagtgc cagcatctaa taaaatagaa
     3301 tttcccggtg aaataagcga aacggcatat tcagctatgc gttctttttc gacaatttgc
     3361 ttaaattgct tctcatttgt tgttggttga aacggtataa attggtttaa gctgacccct
     3421 ccgtgtgtgc gaatcacgac gccttcttca tctaatttta ttaaatcacg gcggatagtt
     3481 gcaggagaag cctcagtcac ttttaccagt tgctctacgg tagcaatatt gtggtttttt
     3541 aggaatagta aaatttgttc tagacggttt ttagacatat aaactctcta atttttaact
     3601 gcttttacta taagtaattt tttaaaaata agatagttaa aattaaagaa cctaaaacat
     3661 tgttgcttta ggttctttgt tgttactagt tcattatatt gcttaagagt gtgttgtttc
     3721 caaatcctcc agatttagtc attacagtga tgcctgagta gttgcccttt tcaatggagc
     3781 ataaaggaac gcccggtact accgcatatt gtacattcat ctctgttaca ttcatttgat
     3841 ctaataatgc tgctgcacca tctccaccaa ataatataag gctatcaaat ttttcattat
     3901 caaggcagaa tttacttaaa tcagctaaat gttgagaaat ttctttagaa gtttccgtga
     3961 tattggcaga ttttgcattt acaacttttg cagggtttgc ccgaataata acatgatctc
     4021 tactaaaacg tattagagcg tttaattgta gtttcagtga atgttctgat tgcgagtagt
     4081 taatcaactg tgaaggcgaa ggattaaata caatagattt tcctcctaaa gttgaaatat
     4141 attcatcaac ttgcagttgg cttgtttcgt gaattgaggt tacgacaatt aatgctctac
     4201 ctaatttaag atttcgctct ggttgttgtt gattttggat atttttcaac ttctgtgcca
     4261 atccggctga gccaacagga ataattttat tacctatgtg atttatgact tgggcaatca
     4321 ttgagagatc ttcgtgcgtt ttagcatcaa taacgatctg tttatattca ctattttcaa
     4381 tagatgcctg caaattatcg accgtatcac acgggattac ttgagcatca ggcaatagtt
     4441 tttgcataga agatgaatgc acagggcaga taggatcttt tcctgaagca gtttcattta
     4501 caggaacacc attaacatat aaatgaccag cttctatagt tcttcccatt tctggatatg
     4561 caggacaaat aattgcttta acatcgggat ataaaccagc ccaagcttta atagcacctt
     4621 caatctgata tttaacagag cctctcattg tggaatcaat tttaaggtaa atttgcccga
     4681 tactattttg aatagcattg ctaattgcaa ttctctccaa ttgctcttga atctgtagaa
     4741 acagaaatta cttgggcttt atcaggatga atttgggcta atgccgaaac tttagttttt
     4801 aagacagtgt caaatcctga ttcagcaaac ataaccgcgg tatcatttgc accagtaagg
     4861 ccatcagcaa taactaagag tgtctctttc atagattttc tccttttata gtgtcttgtt
     4921 ctaagccacc ttgacgtttt aaaacccacg ctgcaaataa tggagctaag attgctgaaa
     4981 ctaatacagc tgcggcaatc tgtgcagtcg cagtttgaac aaaaggtgct aatgtagggt
     5041 ctgcttgagc aacaatttca ggtgtaataa tggcatttcc agcagtagtt cctgctgcaa
     5101 acccgattcc tgattgatta ccacgtctta aaattgtttt gtaaccgata aatacaagaa
     5161 aacctgtaaa gagagaagct acgataccta aaataattcc tgttgctcct ccagtaataa
     5221 tagattgcag attgattcct gttcctaatg caaaagcaaa gaaaggaatg actattgcac
     5281 ctgttggttt cattaactct gtccatttag gatctaagtt accaataacc ataccaataa
     5341 taagaggaat aacagctgca agcattaatg aaagaggaat atctgctaat cctgatacac
     5401 ctaaaaatag taaaacaaag aaaggtccat cattaacagc cgaagcgaca taagctcctt
     5461 tatctctata atcaccatat ttccctgtaa acgctaacca aataccacca tttgaatttg
     5521 caacaacagt tagcatagct aataaagaaa cgcccataac accatcatta cctacccata
     5581 atcctaaaat cacaactaaa agagcgggaa taatagtttt aaatagtaat acaacaccgg
     5641 tatgggcaag tacaggtcca gaacttctca cggtaatttg catacctgta gcgaaaatta
     5701 atagtgcaat aagtggtcct gctgatgatt tgaataaagc cgtcgtaaat gaacctaaat
     5761 ttaaaaattc aggaaagaat gtaccaataa tagaaccaag aattagcggt attaccattg
     5821 caccgccagg aattttattc ataccatcat atatcgggac gatggattta gatgtttgag
     5881 tagacatatt aatctcctta gtgtgtcgca tatttgatat ttgccatttt tgcacccaaa
     5941 tagagtgctt cagtcatact acgactatct gctgttcctt tgcctgctat tgggaaagca
     6001 gttccgtgat cgacagatgt tcgaataaat ggaagaccga cagtaacgtt tacacctgta
     6061 tcaaagccta ataatttgat agggatatgc ccttgatcgt gatacatcac aacaacaata
     6121 tcgaattcat ttaggttagc tgcacggtga aataaagtat ccggagggac aggccctgta
     6181 acattaaacc ctttggcttg agcctcttga attgcgggaa caatttcttt ttgatcttca
     6241 tcgccaaaca ttccattttc acctgaatga ggatttaaac cagcgactgc aatacgaggt
     6301 tctttaaacc caagaatgcg agcattttct tctgctaaat caataactgt agcaactcgt
     6361 tctttggtaa ttaaatcaca ggctttacga agttggacgt gagtagtaac gtgaataaca
     6421 ttgagttttt cactggttag catcattgag taatctttag tattactgag ggttgcaagg
     6481 atctctgtat ggccaggaaa tggtttacca cctgcgtgta gggcttcttt attcagtggt
     6541 gctgtaacaa tagcgtggat ttttcctccc attgctaaat taactgcctc ttcaatatat
     6601 tcataagctg cttttccagc acgcggatca acttgagcaa aaggcaaatc ttcaggcagg
     6661 ttattcaaat ctaaaatatc aataacgcca tattcggcta caaagttatc tagattattg
     6721 agaattctta atttcaattc tgaatgaaca ataggtaagg ctcgttgtat aatttttcga
     6781 tcaccgatca caacagggtg tgctaactca taaatccgtt gattagctaa tgctttaata
     6841 ataacttctg gtccgatacc ggcggcatct cccatcgtaa ttcccaatat aggtttcata
     6901 tttactcctt gattgaaatt aatcattttg aacgaagtat agcttatata aaatcataaa
     6961 agtgtgataa acatcacaaa agtgatcttt tttaatctta ttgatttaat ttaatcaatt
     7021 ttaaggtggt aaattggata gggtatgaaa gtgaaaggca tttattgcta gataaaagct
     7081 tcccttttca ataaaaaaga gtatcatacg ctctcttaaa cgtaacccta catttaagtc
     7141 tgtttccaac ctatcccaat ttctttattc cttaggggca tatagggtaa aaacaaaaaa
     7201 gctgaaacgc tttttttaat aagtagcgag tcaaatgccc catggcatct attcttttag
     7261 gaatagaaag cttaggcttt ccgcaaggca cccgacttat ttttttcacc tcttgtagaa
     7321 ggtttatttt caatgacaat taaaactcct attcaagccg ttcttgcttc aaaccaatat
     7381 tttttagatc gccaagatgc aatggaatca aacgtgcgta gctacccacg taaattacct
     7441 tttgcttacc aaaaagcaca aggcgtttgg gtaactgatg ttgaaggcaa tgaatacctc
     7501 gatttcctag caggtgcagg aacgcttgca ttaggtcata accaccctaa attaatccaa
     7561 gcagttaaag atgtattaga cagtggttta cctttacact cgttagatat tacgactccg
     7621 ttaaaagatg cttttacgga agaattactc tctttctttc caaaagataa atatatcctc
     7681 caatttactg gcccgtctgg tgcagatgct aacgaagcgg caattaaatt agccaaaacc
     7741 tacactggtc gtggcaacgt aattgctttc tccggtggtt tccacggaat gacccatggt
     7801 gcgttatcgc tcaccggtaa tttaagtgcg aaaaatgcag tacaaggctt aatgccgggc
     7861 gtacagttta tgccttatcc gcacgaatac cgctgtccgt ttggcattgg tggcgaagcc
     7921 ggtgcaaaag cagttgaaca ttattttgag aactttattg aagatgttga aagtggtgtg
     7981 gtaaaaccgg cagcggtgat ccttgaagcc attcaaggtg aaggcggtgt ggttccggct
     8041 ccaatcagct tcttacaaaa agtgcgtgaa gtcactcaaa aacacggcat cttaatgatt
     8101 gtggacgaag ttcaagctgg tttctgccgt tcagggaaaa tgtttgcctt tgaacacgca
     8161 ggcattgagc cggatatcgt agtaatgtca aaagcgattg gtggtagctt accgcttgcg
     8221 gtattagcga tcaaaaaaga gtttgacgct tggcaaccgg caggtcatac cggtacattc
     8281 cgtggtaacc aattagcaat ggcaaccggc tatgtatcgc ttaaaattat gtgtgaagag
     8341 aatttagcga aaaacgcaca agagcgtggc gaatacttaa ctaacgcatt acgtgaatta
     8401 agcaaagaat taccttgtat tggcaatgtg cgtggtaaag gcttaatgat gggaattgat
     8461 attgttgatg aacgtcagcc aaaagatgca acaggtgcat acccgcgtga ttgcgattta
     8521 gccgttgcaa ttcaaaaata ctgctttaaa aacaaattgt tattagagcg tggcggacgt
     8581 ggcggtaatg tggtgcgtgt tctttgtgcc gtgaatatta cccaagcaga atgtgaagag
     8641 tttattaaac gctttaaaca atctgttgct gaagcagtga aagcggtgcg tggttaatag
     8701 ttagaatggt aggggcgaaa catttttcgc cctaatgtaa aaaggggcga atatttattc
     8761 gcccctacac caataggata gaaataatgt caaacctaga aaaacacaga caatccctgt
     8821 tttgtagcga tccaaaatcg attgcggatt atgaatcggc aatgaataac gctgtgaaag
     8881 cggtttcaga ctggctgaaa gaagaaaaaa tgtacaccgg tggctcaatt aaagagttgc
     8941 gtgccaaaat caacggtttt aacccaaccc aagctggtgt gggcgtgcaa aaatcacagg
     9001 agcatttggt ggaaattttc ttaaagccaa gcctcaaagt tcaccaccct cattctttgg
     9061 cccacttaca ttgcccgaca atggtaacca gccaaattgc agaagtgtta attaatgcca
     9121 ccaaccaatc aatggactct tgggatcaaa gcccggcagg ttcgattatg gaagagcatt
     9181 taattgactg gttacgtcaa aaagtcggct atggcaaagg aacttcagga gtctttacct
     9241 caggtggtac gcaatcaaac ctgatgggcg tattattggc tcgtgactgg gcgatagcga
     9301 accattggaa aaaggcagac ggttcagagt ggtcggtgca agccgacggt attcctgccg
     9361 atgcaatgca aaaagtgaaa gtggtttgtt ccgaaaacgc tcacttctcg gtgcagaaaa
     9421 atatggcaat gatgggaatg ggtttccaat cggtagtgac tgtaccgtca aatgccaatg
     9481 cacaaatgga cgttgaagct cttgcgaaaa cgttggcgga tttaaaagcc gaaggcaaaa
     9541 ttgtggcttg tatcgtcgca accgcaggga cgaccgatgc cggtgcgatt gatgatttaa
     9601 aagcgatccg taaattagcc gatgaacatc aggcttgggt tcacgttgat gctgcttggg
     9661 gtggtgcatt attactgtcg aaagatttcc gccacttctt agacggcatt gaattaaccg
     9721 attccattac ccttgatttc cacaagcatt tcttccaatc aatttcgtgt ggggcattct
     9781 tgctaaaaga tgaagcgaat taccgcttta ttgattacaa ggcagactac ctgaattcag
     9841 aatatgacga agaacacggc gtgccgaatt tagtgtcaaa atcattgcaa actacccgcc
     9901 gttttgatgc gttaaaatta tggtttaccg ttgaagcctt gggtgaggag ctatacgctt
     9961 caatgatcga ccacggggtt tatctttcta aagaagtgga agcctacatt aatgccaccg
    10021 atggcttgga aatgttagtg ccggcacaat ttgcctcagt cttattccgt gtcgtaccac
    10081 aaggttaccc ggcagaattt gtggatacct taaaccaaaa tgtggcagat gagttatttg
    10141 ctcgtggcga agctaacatt ggtgtgacca aagtcggtga gcatcaatca ctgaaaatga
    10201 ctactttaag cccgattgca acgcttgaaa acgtaagagc attattagct caggtgttgg
    10261 cagaagcaga tcggattaaa gattcgattg taaatggaac ttatgttccg ccaattgatt
    10321 aaactgcgtt aaaaataaaa aggtgttttc gacatttatt tgtgttttaa tgtttgcatt
    10381 cggtagtctt tatcgaatgc aaattttttt aacaaggagt ttttatgaaa aaagttctgg
    10441 gtttaacatt gatggcaatg gcgttatcgg cgtgtaattc aactgcttcc aacggttctt
    10501 cacaagcaaa tacaggtgtt gcaccggcga atgtgattag cgtgaaacag gcgttagcgg
    10561 cagcagatga cagctacgtg acggtagaag gcacactctt aaacaaagtc ggtgacgaaa
    10621 cttacttatt taaagatgcg acagggcaaa tccgtgttga gattgacgat gaagtatggg
    10681 caggtcagca cgttggtagc aaaggcaata aagtccgttt atacggagag attgacaaag
    10741 gtttcaataa aaccgagtta gatgttaaaa cactcactgt aatcaaacct taatttggtt
    10801 ccccgccttg tttggcgggg tttttacgtt aattaatcaa accatccttt tgcaaaaaaa
    10861 cgcaaaatta taccgcttgt tccttcaata ttgtgctaca agatgctgtg atttgctgca
    10921 aaatagcggt gtaatcagtc tcttttaaac caatattacc gaaatagtgc atttttacat
    10981 cacgaatccc acagaactca aacagccctt ttcccagcgt atgttcaaat gctgctaaaa
    11041 agccttttcg ttcatatttt tcattggaat taccgagcgt tacaaattgt tgcatttttt
    11101 tgccggtcag caagccgata ctttcaattt ccccgttacg ataggcaaaa tcataactta
    11161 acaccctgtc taaatagcct tttaaaattg ccggaaagcc catccaccaa agcggataaa
    11221 ttaacgtaat cacatcagct tcaagccaat attgatgctc aattgccaca tctgcttcat
    11281 agtgtttact gagtgtgcct tgaaattccg cccagtctaa tatcggcttg aagttgagtt
    11341 ggtagagatc tcgtacaacc accttatttg gaacggaagc tgctatcgtt ttttccaaaa
    11401 tcgcatggtt aaagctggag ggattcgggt gtgcgtaaat aatcaggtga ttcatagcgt
    11461 tttcctttac tgtgtcgagg cagctactag aagattttag cgaaactttc tttgatgaaa
    11521 atcaatattt tggcgaatat accggcaaat accccgtttg aggaatgaac ggttttaagt
    11581 gaacattaga taatttagcc tattatagtg atgagtaaaa aggtgttttc ttgactgatt
    11641 atcctaatca atcacttcct cgccgtagct ttttgcgagg gcaattttta gactcgttaa
    11701 aaagccaaaa agtcagtcag caagggtatg agccgattcg tccgccttgg gctaatttag
    11761 caaatttttt agaaaaatgc accgcttgcg gtaactgcat tcaggcttgc gaaacccaga
    11821 ttattgtaaa aggtgctgcg ggctaccctg aaattgattt tagccgtggt gaatgcactt
    11881 tttgtgaaaa atgtgtgcat tcttgcgagg cagaggtatt ccgagcagtt tcagaacagc
    11941 cttggagcca taaaattgag attcaagccg gctgtttatt aaagctcgga acggaatgcc
    12001 gaagctgtgg cgatagctgt gagatgagag tgattcgttt ccgcccgagt ttaggcggca
    12061 ttgccgaaat ggtattaaat ttggaaagct gtaatggctg tggggcttgt ttgagcgttt
    12121 gtccgacaag tgcgataaaa attcagccat tacaaacaca ggcaaacaga gatgagtgaa
    12181 aaattatccg aacaggaaaa ctggtatgtg tgcagtctcg tggtgcaagc aaaaccggaa
    12241 aaattggaag aagtaaaagc cgagattctg aagattcctc ataccgaaat tcacggtgag
    12301 aaaacagatg aaggcaaatt ggtcgtgacg attgaaagca atgtgcattt agccctttcc
    12361 gatcgaatcg atcaagtaag agatattaaa ggcgtgattg tggtgtcttt aatttctaat
    12421 tacattgatg aacagtgatt aatgattatt tttgaagcca aacgtgggac atagttatgg
    12481 aactcaatcg tagagatttt atgaaagcga acgccgctgt ggcagctgct gcagccgcag
    12541 gaattatgat tcctgtgaaa aacgttcagg cagatgacac cggtattcgt tgggataaag
    12601 ccccttgtcg ttactgtggt acaggttgta gcgtgctagt cggtactaaa gacggtaaag
    12661 tagtggcaac ccaaggtgac ccggatgcag aagtaaaccg tggtttaaac tgtattaaag
    12721 gttatttctt atccaaaatg atgtacggtg cagaccgtat gcaagagcca atgttgcgta
    12781 tgaaagacgg taagttcgac aagaacggcg atttcacgcc ggtttcttgg gatcaagcct
    12841 ttagcattat ggcggagaag attaaaacca ctattaaaaa tgacggcccg aatgctgtag
    12901 gtatgttctc atcaggtcaa acaaccattt ttgaaggtta tgcgaaagtg aaactttgga
    12961 aaggtggttt ccgttcaaat acgattgacc cgaatgcccg ccactgtatg gcgtccgctg
    13021 cggttgcctt tatgcgtacc tttggtatgg acgagccgat gggctgctat aacgacatcg
    13081 aaaaaaccga tgcgttcgtg ctttggggtt caaatatggc agaaatgcac ccgattttat
    13141 ggtctcgtat ttctgatcgc cgtttatcag acaataaagt gaaagtggtg gtaatgtcta
    13201 cttacgaaca ccgttcgttt gaattagccg atacaccgat tatcttcaaa ccacactcag
    13261 atttagcaat cttaaactac attgctaact acattatcca aaacgataaa gtaaactggg
    13321 attttgttaa caaacacacc aaatttaaac gtggtgaaac caatatcggt tacggtttac
    13381 gccctgaaca caaattacaa caaaatacca atgcgaaaac cgccggtaaa atgtatgaca
    13441 gcgattttga agagtttaag aaaatcgttg ccccttatac attagaagaa gtacaccgta
    13501 tttccggtgt gccgaaagat cagcttgaaa cattagcaaa attatatgca gatcctaacc
    13561 aaaaattagt gtcgttctgg acgatgggct ttaaccaaca tacccgtggt gtgtgggtaa
    13621 accatatgat gtacaacgtc catttattaa ccggtaagat tgcgttagaa ggttgcggac
    13681 cattctcact caccggccag ccgtcagctt gtggtactgc ccgtgaagtg ggtacattta
    13741 ttcaccgttt accggcagat atggtggtta cgaatccgaa acaccgtgag attgtagaga
    13801 aatcgtggaa attaccggct ggagcaattc ctgatgtacc gggcttccac gctacagcac
    13861 aaagccgtgc cttaaaagac ggtaaaatga aattcttatg gcagatgtgt accaacaata
    13921 tgcagggtgg cccgaatatc aatgaagaga ttttcccggg ctggcgtaac cctgaaacct
    13981 ttattgtggt ttcagatccg tacccatctg tttcagcagt ggcagccgac ttaattcttc
    14041 caacctgcat gtgggtggaa aaagaagggg cttacggtaa cgcagaacgc cgtacccaat
    14101 tctggcgtca gcaagtgaaa ggaccggcaa atgcccgctc ggatacttgg cagttagtgg
    14161 agttctctaa atactttaaa gtagaagaag tatggccgga agagttatta gccaaagcac
    14221 ctgaattgcg tggtaaaaca ctatatgaaa tcctttatcg caacggcaaa gtagatagct
    14281 accaagtgcc aaccaacatt ccgggctata tgaatgatga agccgaacac ttcggttact
    14341 acattcaaaa aggcttattt gaagagtatg cggaatttgg acgtggtcac ggtcacgatt
    14401 tagccgactt tgacacctac cacaaagtac gtggtttacg ttggccggta gtaaaagacg
    14461 agaaaaccgg cgaatacaaa gaaactttat ggcgttaccg tgaaggctat gacccatacg
    14521 ttaaagcggg tgaagaagtg gcattctatg gcaatgcgga caaaaaagca gtgattttag
    14581 gtgtgccata cgagccacct gcggaagcac cggacgaaga atatgactta tggttctgta
    14641 ccggtcgagt gttagagcat tggcacaccg gtactatgac ccgtcgagta ccggagttac
    14701 acaaagcatt cccaaccaac ttagtttgga tgcacccaaa tgatgcgaaa aaacgtggct
    14761 tacgtcatgg tgataaagtg aaagtgattt cacgccgtgg tgaatatgtt tctaacttag
    14821 atacccgtgg acgcaataaa tgtccggaag gtttgattta ctcgactttc ttcgatgccg
    14881 gtcagttaat caacaaagtg acacttgatg cgactgatcc aatttcattt gaaaccgact
    14941 tcaaaaaatg tgcggttaaa gtggtgaaag cttaattgta ggggcaaatt atttgcccca
    15001 gacaagcggt caaatttgca aattttttta caaaaacgac cgcttaccta agaggaaccc
    15061 acaatgaaaa tggatccaaa tcgtcgccag tttttaaaaa atgcaacccg taccgcagct
    15121 ggtgtttgcg gtgtgggaat cattttaggg ctacagcaac aacaaagttt tgcccgccaa
    15181 ggggtggcgc tgcgtccacc gggtgcattg ccagagcaag atttcctgag tgcttgcacc
    15241 cgttgcggac aatgtgtgca ggcttgtcct tacgatatgt tgcatttggc atcgcttatt
    15301 tcaccagtgg aagccggcac gccatacttt attgcacggg ataaaccttg cgaaatgtgc
    15361 gtggatattc cctgtatgaa tgcttgccca accggggcat taagcgaaga gctaaaagac
    15421 attgacgatg cacgaatggg gcttgcggtg ttgctcgacc acgaaacttg tcttaactgg
    15481 caaggcttgc gttgtgacgt gtgttaccgt gtttgtccgt taatcgacaa agcgattact
    15541 cttgagaaag tgcataacga ccgtacctcg attcacgcca aactgattcc gaccgtgcat
    15601 tctgatgctt gcaccggttg cggtaaatgt gaacaggctt gcgtattgga agaggcagcg
    15661 atcaaagtgt taccgatgga tttagccaaa ggcttgctcg gtcgtcacta tcgtttaggc
    15721 tggcaagaga aacaaaatgc cggtaaatcg ctgattgaag atgttcaccc agagggttta
    15781 cgcccagctt atgatgccag aatgccggaa ggcttacacg agccggtgta tcagcatatg
    15841 gaagtaaaac ctgatgtgaa agtggctacg ccaagtcggg cgacacaaaa ctatgtgccg
    15901 aactctacga cggtggaagc tccggagcat ttccctgatt tagacttgaa tctcaagggg
    15961 gtgaaataat ggcagccaat tctccaaaaa atgcgggctt agaggctcgt cagaaactag
    16021 gttggtggca cgcttaccgc tttttgattt taagacgctt aagccaactc agcattattt
    16081 tgatgttttt aagcggccca ctttggaatg tatggatttt aaaaggaaac tacagcggta
    16141 gcttattact agataccgtg ccgatgacag acccattaat gaccgctgaa gtgctggcaa
    16201 ccggctatcg ccctgaatgg acggtattgg ttggggcgtt aattatggtg gcgttttacg
    16261 gcattgtcgg cagcaagctg ttttgtagtt gggtttgccc gatgaatatg gtaacagata
    16321 ctgctgcttg gttaagacgc aaactcggta ttcgccaaac tgctaaaatt ccacgcaatt
    16381 tacgctatgt gattttggca gtcattttag tcggtagtgc aatttccggc acgctattgt
    16441 gggagtggat caaccctgtt gcggctctcg gtcgggtgtt tgtattcggc ttaggggcaa
    16501 tgctctggct ggttttagtg gtgttcttgt ttgacttatt ggtagcagag cacggctggt
    16561 gtggtcatct ttgcccgatt ggtgcaactt atgccttaat tggggcgaaa agcctgatta
    16621 aagtcaaagt cgtggatcgt aaccgttgtg ataattgtat ggattgttac aacgtttgcc
    16681 cggaacccca agtgttgcgt gtgccgctac acggtaaacc ggaggaaagc acgattatct
    16741 tatctaaaga ttgcattacc tgcggacgtt gtattgatgt ttgtgccgaa aatgtgtttg
    16801 ccttcggaag tcgttttcaa ggcagtattg atgtgaaaaa tatttaattt ttatcttttt
    16861 gaaaacaaga attttaatgg agtgaacctc aaatgagaaa atatctcgcc ttaattttag
    16921 ctgtgatgtc cggctttgca atggcggaag ccccgaagtt atccggcact attgatgaga
    16981 caaccgaaag tgttgcaccg ggatttcata acccacaaaa agatgtgggc acgattccaa
    17041 ccactttccc tttccaacca ccacttgttc cacatagtat tcgtggctta caagtgagca
    17101 aaaacgtaaa ccattgttta ggttgccacg ctattgaacc atctaaaacc acaggtgcga
    17161 cccgtttacc gccaagccat tttaccgacc gtgatggcaa ggtgtacgaa aacgaatctc
    17221 ctcgtcgtta tttctgtttg caatgccacg tgcaacaaac tgatgtaaac ccgattattc
    17281 aaaataaatt tgaatctgta cgtgaatctc aagctaaata aggagtaagt aatgttgaga
    17341 agattttgta actggttacg tagcccaagc aaaatggcaa ttggtgctgt aatccttatt
    17401 tctgcattag gtggtatttt cgcttggagt ggttttaatg cagggttagc aatgacaaat
    17461 actgaagagt tttgttcaga ttgccatatg aacgatgttg tgccggaata ccgtgcctct
    17521 gctcactatt caaaccgcag cggtgtgaaa gcgatttgtt cagattgcca cgtaccgcac
    17581 gaattctttc caaaatggca acgtaaaatc atagccgcaa aagaagttta tgcacacttt
    17641 atcggtaaag tagataccaa agagaaattt gaagctcatc gtggcgaaat ggcagaacgt
    17701 gaatgggcac gtatgaaagc gaacggctct caagagtgcc gtaactgtca taattttgat
    17761 gatatggact tcactcaaca aaaaaacatt gcgcagcaaa tgcacgcatt ggcacaagag
    17821 caaggtaaaa cctgtattga ctgccacaaa gggattgcac acaatctccc acatatggaa
    17881 gcgatccaga aaaactttat ccctgatgat ttgctgaaaa aagcagaaga gaagaaataa
    17941 cttaacgtgt ttcaatagaa aaccctcaat ttccattgag ggtttctttt tagtagccaa
    18001 gttagtaacc aatggcattg acatttggta aaaaatcgtt ggaatttaac cgcttgacga
    18061 ccgtttctat cgtgccgttt tcgtgcaatt caatttcacg ccagccttgt ggttgtaagt
    18121 ctaaggtaaa ctggtcgcaa ttcggcttaa attgaatgca agtagatggg gtggcataaa
    18181 tcggaatacc atgccaaata tcatcaacct gctgatgaat atgaccatgc acaatcgctt
    18241 ttacattctt aaattttgcg aaaatctcgg ataattcatt gctgtttttt aagctatgct
    18301 gatccagcca cgctgagttt gtcggcaaaa tattgtggtg cagagcaacc aaggtaaacc
    18361 gttgcggata ttgtgccaat ttttcttcta accatttaag ctgtgagtaa gagagttcgc
    18421 cataaggcat acctaccacc tgggtattta gcatcagaag ctgccattgc tcaccgagta
    18481 aaatttgttt atggggaagg atatgtttat attgcgccaa aatttctgac atactcggct
    18541 gaacatcgtg attcccctct agccacacta tcggcgagtt tagcgaatta gttatctgtg
    18601 caaaatagtg ataaccagca atattgtgat cttgaatcaa atcacctgtg gcaagaacta
    18661 gatcaaaata ttgcttcgct tgtttgttta tttgctcaat aactgccgaa aaacttttta
    18721 ctgttttgac attaagtaga gtttcctccg gattggagaa aaggtgaggg tcagttattt
    18781 gtaataactt aattacccct gttacgggta acttaatata ttcgtgcatt cgttttgcat
    18841 ccattcaaat tctagtaatt tttaactggt gagattctat cagtaagcta aaagtaaaaa
    18901 tgtgga
//
LOCUS       DANJWC010000030        18831 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.030, whole genome shotgun sequence.
ACCESSION   DANJWC010000030 DANJWC010000000
VERSION     DANJWC010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18831
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.030"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..271)
                     /locus_tag="PWX02_000776"
     CDS             complement(<1..271)
                     /locus_tag="PWX02_000776"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HDL1728809.1"
                     /translation="MFKFKKQSNQSSSNQENNKISIADELDKLVSLKEKGILSELEFE
                     KEKDKLLNGAKYQAQQPTFIIQNSASSSSSASATAVVKRSGCLSSI"
     gene            complement(294..956)
                     /locus_tag="PWX02_000777"
     CDS             complement(294..956)
                     /locus_tag="PWX02_000777"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728810.1"
                     /translation="MKRAFILLPIIFSSNAFADEMLFSCLTEKNNIISLINKGDMVEY
                     QVASAKTDKITFSFEQPIGNINLSSSVEQGVTQKVYQISNNGYRYDLVVWEKENNKLP
                     VGVEAKDPSGKTVSTNRCKEFIDFINSDTDNKAEENTSSNEPVSNQVYEEGMYRVGRD
                     IPAGEYKLTTTNKERGYFTIYSSSERDSVIAVDTFKNSTYLTVKEGQYLELRNSNAQL
                     VE"
     gene            complement(956..3625)
                     /locus_tag="PWX02_000778"
     CDS             complement(956..3625)
                     /locus_tag="PWX02_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HDL1728811.1"
                     /translation="MSEVIDTLAIEIAANDSFTAVAKPLLSLLERLEGAVNKNTEALD
                     NHGKAAESAAKGTKKLNTEQGKGEKSTKKSTKALQEQEKQIKKNEKVAKNLLQAIGGF
                     TKAIGALGTMILAGVGLDRLVLDTAKANKELAMTSKNLGMTSQSLAAWRGAAELSGGS
                     AQGLTGYLNNLSAGLTRLTVQGDASVTQFFSQLGINLLDGSQKAKKLEDIMLELADKF
                     SSMDRVKAFGIAQQMGIDEGTFEMLAQGRQGLEEHLQKTAKIYKSNQQDLETARKLTA
                     ATAYLNQQFEGLKLMIANAAMPVLLKIADVTNAFFEYLQKNENLVKGVFYGIAGAISI
                     ALIPMLISGASAALAFIAPFLPMILIVAGLAAAIGLLYDDYEKWANGGTSLFNWGLFK
                     KWIFDADFTVGNLKNAFADLLTGYKSWEDAVNAAKSWLELKGFTKDGKMSIDSLATGF
                     KNVAKDIYNSLLPALDKVLNVINLLLDGEFVAAWDAAKELGKDAFNAAVDFVGDNIVG
                     DALKGVGGIIDAGVTNIEEAVNTGKKYWNGEITGSEALKEVTGNFAKNAEKAVDEVVN
                     FADNAITRVENGFVRFVNIWGGFDPDEVGPTGNENKLMDSKKGFSKGNFVFEGQQSVK
                     GLTQEETGALAAQMVKRESGGNLRAENQYGYLGLYQFGAAALVDAGLIDDAKYRAAVR
                     KHGKGLSNGSDADVHKAFLADSSNWTIKGGREAFLSNKAIQDNAIVALMNKNVGYLGS
                     TYQGSAEHKAGLLMAAHLKGAGGAKAFANSGIDSSDGNGTKISDYYNTGRKAIQLAKQ
                     GKSIQSKPINLNTPVGGYAVANALKANQHTFTTMQNLAQPQNISNNQKTEVAFNGGIH
                     INSTAGTLGGTAQDFVNGVQNRVNVMQFNTGLS"
     gene            complement(3625..3771)
                     /locus_tag="PWX02_000779"
     CDS             complement(3625..3771)
                     /locus_tag="PWX02_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728812.1"
                     /translation="MNVSSVVGVVVSSKLATLHELQTIYGLEDALDLIEVFSVDTYNH
                     RDNK"
     gene            complement(3798..4202)
                     /locus_tag="PWX02_000780"
     CDS             complement(3798..4202)
                     /locus_tag="PWX02_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728813.1"
                     /translation="MALKTKKITIEKGRDAGTTFLITEMPIAKADKWASRALLALASG
                     MEGIPSASEGMLGMARVALAAFKQIPEEKALPLLDELLDCVEIVMDSGSTRPIDLAIG
                     DVSDITTLWKLRKEAFALHIDFLDLELTPISE"
     gene            complement(4270..4791)
                     /locus_tag="PWX02_000781"
     CDS             complement(4270..4791)
                     /locus_tag="PWX02_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HDL1728814.1"
                     /translation="MKALKAKFFGSDILVINQNDKPYVPMKQVAENIGLVWHAQFERL
                     QRNEVLSQGIRVIRIPSNGGEQEAVCLPLHYLNGWLFGVNPSRVNPEIKEKLIRYQTE
                     CYEVLWDYWTTGIAKWEDIRQQRENLEENESESKKRGSEAGRALQKRKVEKHTYEIGI
                     ERLDRMEQLLLEF"
     gene            complement(4926..5369)
                     /locus_tag="PWX02_000782"
     CDS             complement(4926..5369)
                     /locus_tag="PWX02_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728815.1"
                     /translation="MRTNIDKTLTAANSILLVRAKGYNKSWVKMEGYAADNAFDFGQG
                     AIGETVIGVDGVQSGGFTPYEVDLNIQLQANSPSRSFFDGVINHINNNQETVPFEFSC
                     EIPSIGKRYSATGFLVNIPGGTNAKKLLESATYSFKVVNNGAEDI"
     gene            complement(5427..6905)
                     /locus_tag="PWX02_000783"
     CDS             complement(5427..6905)
                     /locus_tag="PWX02_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 domain-containing protein"
                     /protein_id="HDL1728816.1"
                     /translation="MFQSIPASRIVNISPAVLSAGGSPLSMNAVFLSKNQNLPTAQAV
                     PFATADAVGEYFGFASEEYQAAAVYFKGFDNSTIKPGTLFFFAYNEAAEGAFLVGNSV
                     KGLSLEELKTVSGSLTINIDGVEKTASSINLRSSKSFSQAAETISSALGDVTVSFDPQ
                     LQSFKISSGTTGSSSAITFATGDVADKLGLSQSVGAVISQGSAETSPTETMQQVIKST
                     LNWATFTTVFEPSLDDKKAFAKWSNDQDQRFLYVAWGKEAGAVQAGNNTCFGAWLKES
                     AYNGTVAIYGGLDKAAFVCGATASIDFTERQGRITYKFKGQAGLTADVTDATVAQILE
                     DNGYNYYGAWATANDRFLFLSPGQIAGKWKWIDAYINQIRLNSQLQLALITLLTSAKS
                     VPYNALGVALQRAACQDPIQEALDFGSIQIGVALSEQQKALINKDTGMDTAAQIEAQG
                     YSLYIGKTSAQVRGKRQSMPMKLWYTDGGSVHAINLASINVQ"
     gene            complement(6921..7427)
                     /locus_tag="PWX02_000784"
     CDS             complement(6921..7427)
                     /locus_tag="PWX02_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728817.1"
                     /translation="MAAEVTLNTTHNDIYREVRAYLLGLFHLEGDQVIRGYSNNAPLP
                     NPPFILMNIIHEQALSTNAYDYSIDEGNVEVMQSLEIQMQLDFYGETSGEISRKFCTL
                     WRNLYACERLERCQPLHCDEPKYLPFTNEASEYEERYMVTAYLTYNPVITHEQDFITN
                     PRISIHSL"
     gene            complement(7412..7792)
                     /locus_tag="PWX02_000785"
     CDS             complement(7412..7792)
                     /locus_tag="PWX02_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728818.1"
                     /translation="MNLRQIANRAITTVNPNIPAVLKLNAGTETDDTGKRVAKFDEIN
                     VKIQPQSLSTNDLQLFDSLAQQGQLLNVYISGQIHALRRLSQQGADKLVFKAFGEKTE
                     SEWLIKSVAESFPNWCKVVIWRQK"
     gene            complement(7800..8504)
                     /locus_tag="PWX02_000786"
     CDS             complement(7800..8504)
                     /locus_tag="PWX02_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728819.1"
                     /translation="MQLTQSHIERINRLWLAMSFDSKEWDESKHPRAANGQFGKDGSQ
                     SAMKSVKANIGRGRSAMNTAIAEKRTVHRAMYHNELGWIDFEWGDTGKIKASGKTKGA
                     MGISHIIESRMRKDNLSYEQATKMLTKNVVEAIAKGKTVDKFEKGNVTALKIDHEKCR
                     VSLRKSKGSNAWIITAFELFEDAGSKGDGKTTATEHQSYSARIDAGASSKNSITPRSI
                     SNQVKKRINSLFQRNR"
     gene            complement(8507..9112)
                     /locus_tag="PWX02_000787"
     CDS             complement(8507..9112)
                     /locus_tag="PWX02_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728820.1"
                     /translation="MSAKFKAALEARIKAVKDANKKVVKVGIVGHQRYDDGTPVAYVA
                     AIHEYGGIFTVPARQATIYRQIDKNGEWTQKNGSRFVSKKKSNFATEVTIPAHTITIK
                     PRPFFRPAFSNNRKKWQKEISNLLKQGHSVEEALELVGSVAAGDVFEALSKVETPKLA
                     ESTIKAKEKRYKSKSDYRPDKPLFASGLLSESISYVVVDKE"
     gene            complement(9109..9540)
                     /locus_tag="PWX02_000788"
     CDS             complement(9109..9540)
                     /locus_tag="PWX02_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HDL1728821.1"
                     /translation="MNITDFRTMFPVFDDLDDPTIELWAEVAEEHLKASWVLNGKRLE
                     IAMRLMTAHLLHTNNVVINADGTAQQNSSGGGIVASASEGSVSVSFATPPTKNAWQHW
                     LAGSPYGLQLWALLKQWSAGGFYIGGLPERKAVRKVGGVFV"
     gene            complement(9543..9917)
                     /locus_tag="PWX02_000789"
     CDS             complement(9543..9917)
                     /locus_tag="PWX02_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728822.1"
                     /translation="MPYIYSTLTCDNAYTIYSDGANGLKVPQGSVIVKGGTGIANDRL
                     ITPLGIITSVSEEELKQLKANPIFKQHLENGFIVVQEDGKAQDAEKVAADMSLDNKDA
                     PVTPSDYQAENAGTKVKNNKGK"
     gene            complement(9987..11117)
                     /locus_tag="PWX02_000790"
     CDS             complement(9987..11117)
                     /locus_tag="PWX02_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HDL1728823.1"
                     /translation="MTKSIQKGHISGRRFATQMAAQKPVIAMDSENVKEFAALAKIGI
                     GFSQDYLNKAPQAWAMDDVQGGVFTGSTGAPIQFLQAWLPGFVHAATAPRKIDELIGM
                     TTVGEWHDEEVVQGVLEATGNAVPYGDLTPIPLANWNVEFARRTVIRFEQGIAVGKLE
                     SARAGAMRLDSAAAKRVAATNALHIERNKVGFYGYNDGNSRTYGFLNDPALLPYNTVA
                     TGKAGGTKWATKTFLEITNDITIAFSQLAAQSNGIIEPKTTPTVLALPTGADVYLGVV
                     SDMGVSVASWLKDTYPNCRVATAPQLVKANGGVDVLYLYAEEVYGDSSDDDNRVWVQA
                     VPAQFMALGAYPEGKIVKEDYTNATAGVMCKRPYAVYRASGI"
     gene            complement(11129..11638)
                     /locus_tag="PWX02_000791"
     CDS             complement(11129..11638)
                     /locus_tag="PWX02_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728824.1"
                     /translation="MTFQQKINIEQGFGVPGDIHLDSPMRAESLVMNSEGSQKNIIGY
                     AFTKDAATNEAKVGGEIASGRVFAGILANSKEYPLHGTEKGTLEPTLALPDHSRGDFV
                     TMGDVVVRVKSACKIGDFVVYDATTGELSTVADKGNLGGKQLVPNAVIYRYPVTNSSG
                     GLTVVRLTN"
     gene            complement(11650..12906)
                     /locus_tag="PWX02_000792"
     CDS             complement(11650..12906)
                     /locus_tag="PWX02_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HDL1728825.1"
                     /translation="MTLAQDKKDIDTNGWFEVKDNPLSKEGVFLYRGSQIRLPDGTQP
                     PDLNQLYPVYRPAEELEKSIPSFRLIPWVDEHTMLGGEEMGMTPAEKKGVNGVIGEDV
                     YFKNGILFGNIKVFSERFARQIENGKKELSLGYRCSYEHSPGVWNGQKYDYIQRDLRG
                     NHLALVEKGRMGADVRVMDSSDVEPNHEQFIFTCDSIMEKKQMTLKELLENAAKQMNE
                     TSSLLSKAAELAEGGAEDDEPTPEQEQEPEMPSQESDTEPEFNEEGQDDEGDVLSKLL
                     DVVERLDARIAKLEGGSGQDDDDMPPEETGKDDDNKQSNEPNAMDAAEITSRAIKAIG
                     ERDALYKKVSAFTGSFNVSAMDSADAVAKYACSKLGLKAPKGQEMAMITGYMANRTPA
                     NKQHQVSVMDGLPASGKSFLDGQINQ"
     gene            complement(12903..14093)
                     /locus_tag="PWX02_000793"
     CDS             complement(12903..14093)
                     /locus_tag="PWX02_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728826.1"
                     /translation="MDDKWITVKPNGEKGKGSPVKIDDEGRIIAGMGGKFKGEKINEI
                     RKSFNGPKTPQRETLETKKQLEKNVQTVTRKTEEKIKSADVPELRSLSKTLENISRSA
                     VATLNNESVQQEYLKLKGKVDKRIAELENLSKPLSIAEKIKALSRTQAKVERVEKSKP
                     FSAQIKEIYSHQRNTEGARDFSKISETDTVNFAKALQKINKQGGYSAQKFNEIATFAW
                     QRESFDVRQLGLNKVADDYVKAGLMKAVDPDRHIYGLTEQGAYIANSVANIVEKEKAN
                     NQPKKKGKSIPALPAGLTPVKIVNEEQVSSWGISTNSNPSASGSNYVKSEATIKKSNA
                     TYLNVPYAEKDGAKALGAKWDKFKKKWYLPAGKEMPEGLKKYATDGFSISERLDILLQ
                     RMKQ"
     gene            complement(14146..14976)
                     /locus_tag="PWX02_000794"
     CDS             complement(14146..14976)
                     /locus_tag="PWX02_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HDL1728827.1"
                     /translation="MHQNNTQIEGKPLIANASTGERYAKSIKRLMRAMHKEALNGIQS
                     CFEAYAEDDDLPKNGSIVAQLRILFSRLLSKYNPIFAILAKKTTARMVQETLKHSTAT
                     IKMSLKEVGKDLTVNPDFMDERLKEITQSGTLEAVSLIKTLPEKYLGDVQKVLTHSIS
                     TKGKGFAELKPFLQKLYKGNERKAELVALDQTRKVYHNVQAHKLQKLGVKKFKWLHSG
                     GGREPRALHVKLSGRVFSFDDPPYIGDMYGEKVYGLPGHLPNCACSMKPVFDFEVEND
                     "
     gene            complement(14951..16462)
                     /locus_tag="PWX02_000795"
     CDS             complement(14951..16462)
                     /locus_tag="PWX02_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HDL1728828.1"
                     /translation="MFGWFKKEEPKQEIEEPKSYWANGKFYRGQPSKLFLDVLANIVQ
                     KNNEINTALSVGQDGDSESRLKMSVRNPEAISVALADWYASKAFIGYQMCAILSQNWL
                     VGKACAVPARDATRNGFDVVSINGDEISDETIKLLQRFDKKYRIRWHCEEFVRLGRVF
                     GMRIALFDIESSDPEFYEKPFNLDGVEPNSYKGIIQIDPYWCVPILVGGELNNPASQH
                     FYEPTYWQINGKKYHRSHLMIFRNDEVPDILKPVYMYGGISTPQKIMERVYSAERTTD
                     ESLGLVTSKRTTVWQTNMDAFMADFDGNREKIQAWIATRDNYGIKVGDKDDDQFSQYD
                     TTLSDLDEVIMTNYQIVAAAANVPVTKLLGTSPKGFSTGAEEAKNYHQELESIQEHDL
                     TEFVERHHQLVMKSFGNEVEDTTINWRPVDSPTAKELAELNKLKADTYSTLIMAGVID
                     GADARTVLVKDREAGFNDLGNVSEDLDLTEEEIALLNSVNVEQLQNAPEQHAD"
     gene            complement(16530..17909)
                     /gene="terL"
                     /locus_tag="PWX02_000796"
     CDS             complement(16530..17909)
                     /gene="terL"
                     /locus_tag="PWX02_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HDL1728829.1"
                     /translation="MFQFSAAQKIVAAELARTDLYFFTRYMFQQKRGYQWLQALHHEL
                     ICAALMRVFRGECKRLIINIPPRYSKTEIAIVNFVAWCFGQVPDCEFIHASYSSTLAV
                     NNSSNIRSLIQHEAYQEIFPNVTLDSEAKNHWTTSQGGAFYATGAGGTITGFGAGKER
                     AGFGGAILIDDPHKADEARSEVMRQNVIDWFQNTVESRKNSPENTPIIVIMQRLHEND
                     LAGWLLNGGNGEEWELLCLPAIQKDGSALWPEKHSIEQLRQMEQAAPYMFAGQYMQRP
                     APLDGGVFKPTQMPIIEALPVGKIKWVRGWDLGATVGGDPTAGVKLGKCEDGSLIIAD
                     IAHGDLGPDERDNMIRNTAALDGKSVRISLPQDPGQAGKTQALYLTRMLQGYPVKTSP
                     ESGDKLTRAEPFASQVNVGNVKMLKGAWNEGLINEMRLFPNGNHDDRIDACSRAYAEL
                     LAKGSSYFG"
     gene            complement(17912..18409)
                     /locus_tag="PWX02_000797"
     CDS             complement(17912..18409)
                     /locus_tag="PWX02_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1728830.1"
                     /translation="MAKKGEVKATSKSRGQPTKYKPEYVTQVEKLCLLGATDKDIADF
                     FEVAESTINNWKIEYPEFLESIKKGKLLADANVANSLYKRALGYEAPDIDIRVIDNQI
                     VETPLIKHYPPDPTSAIFWLKNRQPDKWRDKQIQEVSGTVTSQNINLSNDEFRAIAKE
                     LLEKV"
BASE COUNT         4889 a         4477 c         3488 g         5977 t
ORIGIN      
        1 ttatgctaga taaacaaccc gaacgcttaa ccaccgcagt tgcagaagct gacgaagatg
       61 aagatgctga gttttgaata ataaacgtag gttgttgagc ttgatattta gctccattta
      121 acaacttatc tttttctttt tcaaattcca gttctgataa aatacctttc tcttttaacg
      181 aaacaagttt gtccaattca tcagcaatag atattttatt attttcttgg ttactgctac
      241 tttgattaga ttgtttttta aatttaaaca taatagacct caaattaacc ggattattcc
      301 acaagctgtg cattactatt tctaagttct aaatactgcc cttctttcac cgtgagatat
      361 gttgaatttt taaaagtatc aacagcaatt acagaatcac gctcagagct agaataaatt
      421 gtaaaataac cacgctcttt atttgtggtt gttaatttat actctcctgc tggaatatct
      481 cgcccgacac gatacatccc ttcttcataa acctgattgg atacaggttc attgctcgaa
      541 gtattttcct ctgctttatt atcagtatcg ctatttatga aatctataaa ttccttacaa
      601 cgattagtcg atactgtttt gccgctagga tctttagcct ctaccccaac agggagttta
      661 ttattttctt tttcccacac aacaagatcg tatctatatc cattgtttga tatctgatac
      721 accttttgag taacaccttg ctcaacagaa gaagaaagat tgatattccc tattggctgt
      781 tcaaatgaaa atgtaatttt atccgtttta gcgctagcaa cttgatattc aaccatatcc
      841 cctttgttga tgagactaat gatattattc ttttctgtta aacaagaaaa caacatctca
      901 tcagcaaaag cattggaaga aaaaattata ggtaaaagga taaatgctct tttcattatg
      961 acaatcccgt attaaattgc atcacattca ctcggttttg caccccatta acaaaatctt
     1021 gagctgttcc gccaagtgtt cccgctgttg agtttatatg aattccccca ttaaatgcaa
     1081 cttctgtttt ttggttgttg ctaatatttt gcggttgagc caagttttgc attgtggtaa
     1141 aggtatgctg attagctttt agtgcatttg ctactgcata gccaccaact ggcgtattca
     1201 aattgatagg cttgctctga atagatttac cttgtttagc taactgaatc gcctttcgcc
     1261 ctgtattata gtaatcagat atttttgttc cattcccatc tgagctatct atacctgaat
     1321 ttgcaaaagc tttcgctccg ccagctcctt ttaagtgagc tgccatcaat aatccagctt
     1381 tatgctccgc agaaccttga taagtagagc ctaaatatcc aacattctta ttcataagag
     1441 ctacgattgc attatcttga atagctttat tgcttaagaa agcttctcga ccacctttaa
     1501 tagtccagtt gcttgaatct gctaaaaatg ccttgtgaac atcagcatca gaaccattag
     1561 ataatccttt accgtgtttt cttactgctg ctctatattt tgcatcatca attaaccctg
     1621 catcaactaa tgcggccgca ccaaactgat ataatcctaa atagccgtat tgattttccg
     1681 ctctgaggtt tccaccactt tcacgcttca ccatttgagc agccaatgcc cctgtttctt
     1741 cttgcgtaag acctttaaca gattgttgcc cctcaaagac aaaatttcct ttactaaatc
     1801 ctttcttgct atccatcagt ttgttctcat taccggtagg accaacttca tctgggtcaa
     1861 atccgcccca aatattcaca aatcgaacaa atccattttc tacccttgta atggcgttat
     1921 ctgcaaaatt aacaacctca tcgactgctt tctcagcatt cttggcaaaa ttccctgtta
     1981 cttctttaag agcttctgag cctgtaatct cgccattcca atatttttta cccgtattga
     2041 cagcctcttc tatattagta acgccagcat ctattattcc accgacacct ttcagtgcat
     2101 caccaacaat attatcacca acaaaatcta ctgcggcatt aaacgcgtct ttccctaact
     2161 cttttgctgc gtcccacgct gcaacaaatt ctccatcaag taataggttg atgacattta
     2221 atactttatc tagtgcagga agaagcgagt tatatatatc tttcgccaca tttttgaaac
     2281 ctgttgccaa gctatctata gacatcttcc catctttggt aaagccttta agttctagcc
     2341 aagacttagc agcattaacc gcgtcttccc aagatttata accggttaat aaatcggcaa
     2401 acgcattttt cagattgcca acggtaaaat ctgcatcaaa aatccacttc ttaaataatc
     2461 cccagttaaa tagcgaagta cctccgttcg cccacttttc ataatcatcg tagagcaatc
     2521 caatagctgc tgctaacccc gcaacaatta aaatcattgg caggaatgga gcaataaacg
     2581 ctaaagcagc acttgcaccg ctaataagca ttgggattag agcaatagag atcgcccccg
     2641 caattccata aaataccccc tttacaaggt tttcattttt ctgcaaatat tcaaaaaacg
     2701 cattggttac atcggcaatt ttgagtaata ccggcattgc ggcattggca atcatcagct
     2761 ttaaaccttc aaattgctgg tttaaatagg cagttgctgc ggtcagtttg cgtgcggttt
     2821 ctaaatcctg ttgattggat ttgtagattt tagcggtttt ctgcaaatgc tcttcaagac
     2881 cttgtctgcc ctgcgccagc atttcaaacg taccttcatc aatccccatt tgctgggcaa
     2941 tgccaaaggc ttttactcta tccattgagc tgaatttgtc tgccaattcc agcataatgt
     3001 cttcaagttt tttggctttt tgtgagccgt ctaatagatt gatacccagt tgagaaaaaa
     3061 actgagtaac agaggcatca ccttgtacag ttaaacgggt aagaccggca gaaaggttat
     3121 tcagataacc ggtaagcccc tgtgcagaac cgcctgaaag ctctgctgca cctcgccaag
     3181 ctgctaacga ttggctggtc attcccaggt tcttagatgt catagccaat tctttgttag
     3241 ctttggctgt atcaagcaca aggcggtcaa gtccaacgcc agccaaaatc attgtgccta
     3301 atgcaccaat cgctttggta aaaccgccaa tcgcttgcag taagttctta gcgacttttt
     3361 catttttctt gatctgcttt tcctgctctt gcagggcttt agtcgatttt ttagtggatt
     3421 tttcgccttt accctgctca gtattgagct ttttagtgcc tttggcggca ctttcagccg
     3481 ctttcccgtg gttgtctaag gcttcggtgt ttttattgac cgctccctct aaacgttcca
     3541 atagggataa taacggcttt gctactgccg taaaactatc attcgccgca atttctatcg
     3601 ctaatgtatc aataacttct gacattattt attatcccta tggttataag tatccacgct
     3661 gaacacttca attagatcta gagcatcttc cagcccataa atagtctgta attcgtgcaa
     3721 ggttgccagc ttactactta ccacaacccc aacaaccgaa cttacattca catacgcaac
     3781 agcattgctt tctttactta ctccgagatc ggggtaagtt cgagatccaa aaaatcaata
     3841 tgcaacgcaa acgcctcttt tcgcaatttc caaagcgttg tgatgtcact cacatcacca
     3901 atcgctaaat caatcggtct ggttgagccg ctatccatca cgatttctac acaatccaat
     3961 agttcatcta ataacggtaa ggctttttct tccggaatct gcttaaaagc cgctagtgca
     4021 actcttgcca ttcccagcat cccttccgaa gctgacggga taccttccat tccactcgcc
     4081 aaggcaagta atgcacgaga agcccacttg tccgctttgg caatcggcat ttccgtaatc
     4141 aaaaacgtcg tgcctgcatc tcgtcccttt tcaatcgtga ttttttttgt ttttaaagcc
     4201 atttttttat tcctctgagg tggttaaata tctaagaaca gatccatttg ctgaatttac
     4261 cctatatctt taaaattcaa gtaacagctg ttccattcta tctagtctct ctatgccaat
     4321 ttcataggta tgcttttcta cttttcgttt ttgcaacgct cgcccagcct cactgccacg
     4381 cttttttgat tcactttcat tttcttctaa gttttcacgt tgttggcgga tgtcctccca
     4441 tttggcaatg ccggttgtcc aataatccca taggacttcg taacactctg tttgataacg
     4501 aatcagcttt tctttgatct cgggatttac acgagaagga tttacaccaa acagccagcc
     4561 attaaggtaa tgtaacggca aacaaactgc ttcttgctca ccaccatttg aaggtattcg
     4621 tataacacga ataccttgtg aaagtacttc atttcgttgt aatcgctcaa attgagcgtg
     4681 ccaaactaaa ccgatatttt ctgccacctg cttcattggt acatatggct tatcattttg
     4741 attgataact aaaatgtctg aaccaaaaaa ttttgctttt aatgctttca ttattttttc
     4801 cttttatgaa ttttggataa taaaaaaccc gaccatttct gatcgggttg tgaattaccg
     4861 caacattcct accttattac aggctcgtaa actcacgatt taaagctgtt tgggaacatt
     4921 aaaggttaaa tatcttctgc accgttattc acgactttga atgaataggt cgcagattca
     4981 agcaatttct tcgcattcgt accgcccgga atgttcacta aaaagccggt tgccgaatag
     5041 cgtttgccga tagacggaat ttcgcacgag aattcaaacg gcacagtttc ttggttgttg
     5101 ttgatatggt taatcacacc atcaaaaaaa gaacggctcg ggctgttggc ttgaagttga
     5161 atatttaaat cgacttcata aggcgtaaat ccgcctgatt gcacaccatc aacgccgatc
     5221 acggtttcgc cgattgcacc ttgcccgaag tcaaaggcat tatcggcggc gtatccttcc
     5281 attttgaccc acgatttgtt ataacccttt gcccgcacaa gtagaatgct gtttgcggcg
     5341 gtcagagttt tatcaatatt tgtacgcata atttattcgt ttcctttttt tgcaaaaaag
     5401 cggtcgaatt tgaccgcttg ttactgttat tgaacgttaa tagaagcaag gttaatggcg
     5461 tgaacactgc cgccgtcggt gtaccataac ttcatcggca ttgactggcg tttacctcgc
     5521 acctgtgccg aagttttgcc aatatagagt gaataacctt gtgcctcaat ttgggcggcg
     5581 gtatccattc cggtgtcttt attgattaag gctttttgct gttcgcttaa tgccacacca
     5641 atttggatag agccgaaatc taacgcctct tgaatcggat cttgacaagc cgcacgttgt
     5701 aaagccacgc cgagagcgtt atatggcaca gacttcgccg aagtaagcaa ggtaatcaag
     5761 gcaagttgta attggctatt caggcgaatt tgattgatgt aagcgtcaat ccatttccat
     5821 ttgccggcaa tctgacccgg cgacaagaac aagaagcggt cgtttgcggt tgcccaagct
     5881 ccataatagt tgtagccgtt atcttccaaa atctgtgcaa cagtggcatc agttacatcg
     5941 gcagttaaac cggcttgccc tttgaattta taagtaattc gtccttgtcg ttcggtgaaa
     6001 tcaatagaag cggttgcacc acacacaaaa gcggctttat ctagtccgcc ataaatagca
     6061 actgtgccgt tataagcaga ttcttttaac cacgctccga aacaagtgtt attaccggct
     6121 tgtaccgcac cggcttcttt gccccaagca acatacaaga aacgctgatc ttggtcgtta
     6181 ctccatttcg caaaggcttt tttgtcgtct aaacttggct caaatacggt ggtaaatgtc
     6241 gcccagttta aagtggattt gatcacctgc tgcattgttt cagtcggcga tgtttcagca
     6301 ctaccttggg aaataaccgc accgactgat tgcgataagc ctaacttgtc tgcaacatca
     6361 ccggttgcga aagtgatagc agatgatgaa cctgtggtgc cggatgaaat cttgaatgat
     6421 tgcaactgcg gatcaaaaga aaccgttaca tcgcccagtg cggaagaaat agtttctgcc
     6481 gcctgcgaaa aactttttga tgaacggagg ttaattgacg aagcggtctt ctctacacca
     6541 tcaatattga tagtaagaga gcctgaaacc gttttcaatt cttccaatga caaccctttt
     6601 actgaattac caaccaagaa agccccttct gctgcctcgt tgtaggcaaa gaaaaacagt
     6661 gtgccgggtt tgatggttga gttgtcaaag cctttaaagt aaactgccgc cgcttgatac
     6721 tcttctgagg cgaagccgaa gtattcccct accgcatcag cagtcgcaaa cggtacggct
     6781 tgagcagtcg gcagattttg atttttgctt aaaaacacgg cattcattga gagtggcgaa
     6841 ccaccggcac ttaataccgc aggactgata ttcacaatcc gggaagcagg aatagattga
     6901 aacatagcta tgttgttctc ctataaagaa tgaatagaaa ttcttgggtt ggtaataaaa
     6961 tcttgttcgt gagtgatcac agggttgtag gttaaatatg ccgttaccat atatctctcc
     7021 tcgtattcgc tggcttcatt ggtaaacggc aaatatttcg gttcgtcaca atgaagcggc
     7081 tggcaacgct ccaatcgctc acaggcgtat aaattccgcc ataatgtgca aaatttccgt
     7141 gaaatttcac cgcttgtctc gccgtaaaag tcaagttgca tctgaatttc aaggctctgc
     7201 atcacttcta cattgccttc atcaatgctg taatcataag cattagtgga taaggcttgt
     7261 tcgtgaataa tattcatcaa aataaacggc ggatttggta gcggtgcgtt attgctatag
     7321 cctcgaataa cctgatcgcc ctctaaatga aaaagcccga gcaaataagc ccggacttct
     7381 cgataaatat cattatgagt ggtgtttaag gttacttctg ccgccatatc accaccttgc
     7441 accagttagg aaaactttcg gcaacggatt taatcagcca ttctgattcc gttttttcgc
     7501 caaaagcctt aaacactaat ttatccgctc cctgttgaga aaggcggcgt agggcgtgaa
     7561 tctgccctga aatataaaca ttaagcaact gaccctgttg ggcgagactg tcaaaaagct
     7621 gtaaatcatt ggttgaaagg ctttgaggct gaattttgac gttgatttca tcaaactttg
     7681 ccactctttt gccggtgtca tccgtttctg ttccggcatt gagtttcaat actgccggaa
     7741 tattcggatt gactgtggtg atcgcacgat ttgcgatttg gcgtaggttc attgttcatt
     7801 tacctatttc tttggaaaag cgaatttatt cgttttttca cttgattaga aattgatctt
     7861 ggggttatac tgtttttgct tgaagctcct gcatcaatac gagcggaata ggattgatgt
     7921 tcagtagcgg tagtcttgcc atcccccttg ctacctgcat cttcaaataa ctcaaaggca
     7981 gtaataatcc aagcattact gccttttgat tttctaagag atactcggca tttctcgtgg
     8041 tcgattttaa gtgccgttac gtttcctttc tcgaacttat caacagtctt ccctttagca
     8101 atggcttcca cgacattctt tgtcagcatt ttggtagctt gttcataact taaattatct
     8161 tttctcatac gagattcaat aatgtgagat atacccatag cacctttggt tttcccactg
     8221 gctttaattt ttccagtatc accccactca aaatcaatcc agccaagttc gttatgatac
     8281 attgcacgat gaacagttct tttttctgcg attgcggtat tcatcgctga gcgacctcgc
     8341 ccgatattcg ctttcacaga cttcattgca gattgtgagc catctttccc aaactgcccg
     8401 tttgccgccc tcgggtgttt gctttcgtcc cactctttgc tgtcaaatga cattgccagc
     8461 cataagcgat taatccgttc aatgtgtgat tgtgtcagtt gcacaactac tccttatcta
     8521 caacaacata tgagatggat tctgaaagca agccacttgc aaaaagaggt ttgtctgggc
     8581 ggtaatctga tttagattta taacgctttt ctttcgcttt aattgtactc tcagctaatt
     8641 tgggtgtttc tactttgctt agtgcctcaa atacatcacc ggcggcaaca ctaccgacaa
     8701 gctctaatgc ctcttctaca gaatgccctt gctttaatag gtttgaaatt tctttttgcc
     8761 actttttcct attattggag aaagcaggtc taaaaaaagg tctcggctta atggtgattg
     8821 tatgagcagg aatagttact tctgtcgcaa aattcgattt tttcttgctg acaaagcgac
     8881 taccattttt ctgtgtccat tcgccatttt tatctatttg tcgataaata gtcgcctgtc
     8941 tggcaggaac agtaaatatt ccgccgtact cgtgaatcgc cgcaacataa gcaacaggcg
     9001 tgccatcatc atagcgttga tgtccaacaa taccgacttt aaccaccttt ttatttgcgt
     9061 ctttcacggc tttaatccgt gcctccaacg ctgctttaaa ttttgcactc acacaaacac
     9121 cccaccgact ttccgcactg ctttacgttc cggcaaaccg ccgatataaa aaccacctgc
     9181 agaccattgt tttagcaacg cccacaactg caagccatag ggcgaaccgg caagccaatg
     9241 ctgccaagcg tttttagtcg gcggtgttgc gaatgaaacg cttacgctac cctcggaagc
     9301 agaagcaaca atgccaccac cgctgctatt ttgttgtgct gtgccgtctg catttatcac
     9361 aacattgttg gtatggagta aatgagcggt cattaatcgc attgcaatct ctaaccgctt
     9421 gccgtttaat acccaacttg cttttaaatg ttcttccgcc acctccgccc aaagctcgat
     9481 tgttggatcg tctaaatcat caaatacagg aaacattgtg cgaaagtcgg tgatgttcat
     9541 tgttacttac ctttgttgtt ttttactttg gtgccggcat tctcagcttg atagtcagac
     9601 ggcgtaaccg gtgcatcttt attgtctaac gacatatccg cagccacttt ttcagcatct
     9661 tgtgctttgc cgtcttcctg caccacaata aaaccatttt ctaaatgctg tttaaaaatc
     9721 gggtttgctt tgagttgttt aagttcttct tccgataccg aagtgataat ccctaatggt
     9781 gtgattaaac ggtcattcgc aatccctgtg ccacctttaa caattacgct accttgtggc
     9841 acttttaagc cgtttgcacc gtctgaataa atggtataag cattatcaca agttaaggtt
     9901 gaatagatat aaggcattat gtttctcctg tttaggtaat aaaaaaccgc tcataaagag
     9961 cggtcgtttt ttgttgtttt tttgcattaa atgccggaag ctcgataaac agcataaggg
    10021 cgtttacaca ttacaccggc agttgcgttg gtgtaatctt ccttcacaat tttgccttca
    10081 gggtaagcac cgagagccat aaactgtgcc ggtacggctt gcacccaaac acgattatca
    10141 tcgtccgagc tgtcgccata aacttcttca gcgtagagat ataacacatc aacgccacca
    10201 ttcgctttta ctaattgcgg agcagttgct acacgacaat ttgggtaagt gtcttttaac
    10261 cacgaagcta cgcttacgcc catatcagac acaacaccaa gatatacatc cgcaccggtt
    10321 ggtaatgcta ataccgttgg cgtagttttt ggctcaataa tgccgtttga ttgggcggct
    10381 aattgcgaaa acgcaatggt gatgtcgtta gtaatttcta agaacgtctt agttgcccat
    10441 tttgtgccac ctgctttgcc ggttgcaacg gtgttatatg gcaataatgc cggatcgttt
    10501 aagaaaccgt aggtgcgaga gttgccatcg ttatagccgt agaaaccgac tttgttacgc
    10561 tcaatatgta acgcattggt tgccgccaca cgttttgcag ctgcactgtc taaacgcatt
    10621 gcaccggcac gggcagattc taatttacct accgcaatgc cttgttcaaa gcgaattacg
    10681 gtacggcgtg caaattctac gttccagttt gctaacggaa ttggcgttaa gtcgccgtat
    10741 ggtactgcat tgcctgtggc ttctaacaca ccttgaacga cttcttcatc gtgccactcg
    10801 cccacggtcg tcataccgat taactcatcg attttacggg gtgcagttgc agcgtgaaca
    10861 aaacccggta gccacgcttg caagaattga atcggtgcac cggtgctacc tgtaaatact
    10921 ccgccttgca catcatccat cgcccaggct tgtggcgctt tatttaagta gtcttggcta
    10981 aagccaatac cgatttttgc cagtgctgca aactccttaa cattttcaga gtccattgca
    11041 atcaccggtt tctgtgccgc catttgagtt gcaaaacggc gaccgctgat atgccctttt
    11101 tgaattgatt tagtcatcta tcatttcctt aatttgttaa acgaactacg gttaaaccgc
    11161 cgctactatt ggttaccgga tagcgataaa tcaccgcatt cggcactaac tgtttaccac
    11221 ctaaattgcc tttatctgca acggtagaaa gttcgccggt agtcgcatcg tacacaacaa
    11281 aatcgccgat cttacacgca gatttcacac gaacgacgac atcacccatc gtaacgaaat
    11341 caccacgact gtgatctggc aatgctaatg tcggctctaa tgtgcctttt tctgtaccgt
    11401 gtaatgggta ttctttggag tttgccaaaa tacctgcaaa cacccgacca cttgcaattt
    11461 caccgcctac ttttgcctca ttggttgcag cgtctttagt gaaagcgtaa ccaataatgt
    11521 ttttttgtga accctctgag ttcattacaa ggctttctgc acgcattggg gaatcaagat
    11581 gaatatcacc cggtacacca aagccctgtt caatattgat tttttgttga aatgtcattt
    11641 ctacctacct tattgattga tttgaccatc aaggaatgat ttaccgcttg ccggtaagcc
    11701 gtccattaca ctcacttggt gctgtttatt tgccggcgta cggtttgcca tataaccggt
    11761 aatcatcgcc atttcttgcc cttttggggc ttttaagcct aatttcgagc aagcatattt
    11821 agctactgca tcggctgagt ccattgctga tacattaaac gacccagtaa atgcagagac
    11881 tttcttatac agagcatctc gttcaccaat agctttgata gcacgactgg taatttccgc
    11941 cgcatccatt gcatttggtt catttgactg cttgttgtca tcgtccttac cagtttcttc
    12001 cggtggcata tcgtcatcat cttgaccgct gccaccttct aatttcgcaa tacgggcatc
    12061 taaacgctcg actacatcaa gcaatttgct taacacatcg ccttcatcgt cttgcccctc
    12121 ttcattaaat tcaggttcag tatcagactc ttgacttggc atttcaggct cttgctcctg
    12181 ctcaggcgtt ggctcgtcat cttccgcacc accttccgcc aattctgcgg ctttgctcaa
    12241 aagtgaggag gtttcattca tctgttttgc tgcgttttcc agcaattctt ttaatgtcat
    12301 ttgttttttc tccattatgg aatcacaagt aaaaataaac tgttcgtggt ttggctcaac
    12361 atcactgcta tccataactc gaacatctgc ccccattctg cctttttcga ctaaggctaa
    12421 atgattgccc cttaaatccc gctggatata gtcatatttc tgcccattcc aaacgccggg
    12481 actatgttca taactgcacc gataaccgag agaaagctct ttcttgccgt tctcgatttg
    12541 tcgggcaaac cgttctgaaa acaccttaat gtttccgaat aatatgccgt ttttaaaata
    12601 aacgtcttcc ccaattaccc catttacgcc tttcttttca gccggtgtca ttcccatttc
    12661 ctcaccgcct aacatcgtgt gttcgtccac ccacggaatt aagcgaaagc tagggattga
    12721 cttttccaac tcctcagcag ggcggtaaac agggtaaagt tggtttaaat ccggtggttg
    12781 tgtgccgtca ggtaatcgaa tttgcgaacc ccgataaaga aaaaccccct ctttgctgag
    12841 ggggttgtct ttaacttcaa accaaccgtt tgtgtcaata tcttttttgt cttgtgcgag
    12901 tgtcattgtt tcatcctttg caacaaaata tctaagcgtt cggagattga aaaaccatca
    12961 gtcgcatatt tctttaaccc ttccggcatt tctttacctg ccggtaaata ccatttcttc
    13021 ttaaacttat cccattttgc accaagagct tttgccccgt ctttttcggc gtatgggaca
    13081 tttaagtaag tagcattaga tttttttatt gtagcttctg atttaacata attactccca
    13141 gaagctgacg gattactgtt tgttgatatt ccccaagagc ttacctgctc ctcatttaca
    13201 attttcacag gagttaaccc agcaggaaga gccggaatgc ttttaccttt tttcttaggt
    13261 tgattgttcg ctttttcctt ttcaacaata ttagcaacac tatttgcaat ataggcacct
    13321 tgctctgtta gtccataaat atgcctatca ggatcgactg ctttcatcaa acctgctttg
    13381 acataatcat cagcaacctt atttaagcct aattgcctta catcaaaact ttctctttgc
    13441 cacgcaaatg ttgcgatttc attaaatttc tgagctgaat atccaccttg tttgttaatc
    13501 ttttgcaatg cttttgcaaa attaacggta tcagtttcac ttatttttga aaaatctctt
    13561 gctccttcag tatttcgttg atgggaataa atctctttaa tttgagcaga gaaaggtttt
    13621 gacttttcca cccgttctac ctttgcttga gtacgtgata aagccttaat tttttctgct
    13681 atacttagtg gtttagataa attttctaac tcagcaattc ttttatcaac cttgcctttc
    13741 aacttcaggt attcttgctg tacgctttca ttgtttaaag tcgctacggc acttcgagag
    13801 atattttcta acgtcttaga taaacttcgt agttcaggca catccgcact cttaatcttt
    13861 tcctcagttt tacgagttac tgtctgaacg ttcttttcaa gttgtttctt tgtttctaat
    13921 gtttctcgct gtggcgtttt tggcccatta aaactcttac gaatttcgtt aattttctcg
    13981 cctttgaatt tgccacccat accggcaatg attcgacctt cgtcgtcaat tttgaccggt
    14041 gagcctttac ctttttcgcc gttaggctta actgtgatcc atttatcgtc cattgcgaca
    14101 accgaaaaca gtctttcaat acggtttcta tgtgattgtg tgatgttaat cattttctac
    14161 ctcaaaatcg aagacaggct tcatactgca cgcacaattt ggcagatgac cgggcaaacc
    14221 ataaaccttt tcgccgtaca tatcgccaat ataaggcggg tcatcaaacg aaaatacccg
    14281 accgcttagt ttaacgtgta aggccctcgg ctcacgccca ccgcctgaat gtagccattt
    14341 gaattttttt acacccaatt tttgcaattt atgagcctga acattgtgat aaaccttacg
    14401 agtttgatcg agagcaacca gttccgcttt tcgctcattc cctttgtaga gtttttgcaa
    14461 aaacggtttt aattccgcaa agcctttccc ctttgttgaa atcgagtgtg tcagcacctt
    14521 ttgtacatcg cccaagtatt tctcgggcaa ggtttttatt agcgataccg cttcaagcgt
    14581 gccggattgg gtaatttctt tcagtcgctc atccataaaa tcaggattaa ccgttaaatc
    14641 tttgccgacc tctttcaagc tcatcttaat ggttgcagta gagtgtttga gcgtctcctg
    14701 caccattcta gcggttgttt ttttggctaa aattgcaaaa atcggattat acttacttaa
    14761 aagccgacta aacaaaatcc gcaactgcgc cacaatcgag ccgttttttg gcaggtcatc
    14821 atcttcggca taagcctcaa agcaagattg aataccattt aacgcctctt tgtgcattgc
    14881 tcgcattaac cgcttaatgc ttttggcata acgctcacct gttgaagcat tggctattag
    14941 tggcttgcct tcaatctgcg tgttgttctg gtgcattttg cagttgctcc acattgaccg
    15001 aattaagtaa tgcgatttct tcttcagtta aatctaaatc ttcgctgaca ttgcctaaat
    15061 cattaaaacc ggcttctcgg tcttttacta acacagttcg agcgtcagca ccatcaatta
    15121 caccagccat aattagcgta gagtaagtat cggctttcag cttattcaac tctgccagct
    15181 cttttgctgt tggagaatca accggtcgcc agttaattgt ggtatcttct acctcattac
    15241 caaatgattt catcacaagc tgatgatgac gttctacaaa ctcggtcaaa tcgtgttctt
    15301 gaatactctc taactcttgg tgatagttct tcgcctcttc cgcaccagtc gaaaatcctt
    15361 tcggtgatgt gccaagtaat tttgttaccg gcacatttgc agcagcggct actatttgat
    15421 agttagtcat aatcacttca tcaaggtcgc tcaatgtcgt gtcgtactgg ctgaactggt
    15481 catcgtcttt atcgccaacc ttaataccgt agttatcacg agttgcaatc cacgcttgaa
    15541 ttttttcccg attgccgtca aagtccgcca taaaagcgtc catattggtt tgccatacgg
    15601 ttgtgcgttt tgaggtaact aatcccaacg attcatcggt tgttcgctcc gcactgtaaa
    15661 cccgttccat aatcttctgc ggcgttgaaa taccgccata catatagaca ggctttaaaa
    15721 tatccggcac ttcatcattg cggaaaatca tcaagtgcga acggtggtat tttttaccgt
    15781 tgatttgcca ataggtcggt tcgtagaaat gctgacttgc cgggttattc aactcaccgc
    15841 cgaccaagat aggcacacac cagtaaggat cgatttgaat aatccctttg tagctgtttg
    15901 gttctacacc gtctaaatta aacggttttt cataaaattc aggatcgctt gactcaatgt
    15961 caaacaatgc aatccgcata ccaaacacac gccctaagcg tacaaattct tcacaatgcc
    16021 agcggatgcg gtatttttta tcaaagcgtt gaagcagctt gattgtttcg tctgagattt
    16081 catcaccatt gatagacacc acgtcaaaac cgtttctggt agcatctctt gccggtacag
    16141 cacaggcttt acctacaagc cagttttgcg acaaaatcgc acacatctga tacccgataa
    16201 atgcctttga agcataccaa tccgccaagg caacgctaat cgcttcggga tttcgcaccg
    16261 acatcttcaa gcgtgattca ctatcgccat cttgaccgac agaaagtgcg gtattgattt
    16321 cattattttt ctgaacaata ttcgccaaca catcaagaaa cagtttagac ggttgcccac
    16381 gatagaattt accgttagcc caataggatt taggctcttc tatttcttgt tttggctctt
    16441 cttttttaaa ccaaccaaac atatttctct ctgtaataaa aaaccgcctg tattgctaca
    16501 agcggttgga tttcaataaa attttgcaat taaccaaaat atgaagaccc tttcgccaac
    16561 agctctgcat aagcacgaga acaagcgtct atgcggtcat cgtgattgcc gttcgggaat
    16621 aaccgcattt cattgattaa tccctcattc cacgcccctt taagcatttt cacgttacct
    16681 acattcactt gtgaggcaaa cggctcggct cgggtgagct tgtcgccact ctcagggctg
    16741 gttttaacgg gatagccttg tagcattcgg gttaaataca gggcttgagt tttcccagct
    16801 tgccccgggt cttgtggtaa gctaatacgc acacttttcc cgtctaaggc tgccgtgttg
    16861 cggatcatat tatcccgttc gtccggccct aaatcgccgt gtgcaatatc tgcaatgatc
    16921 agcgaaccat cttcgcattt gccgagctta acaccggcgg tcggatctcc gcccactgtt
    16981 gcccccaagt cccagccacg cacccatttg attttaccta ccggcaacgc ttcaatgatc
    17041 ggcatttgcg ttggcttaaa tacaccgccg tcaagtggtg ctgggcgttg catatattga
    17101 ccggcaaaca tatagggagc tgcttgttcc atttggcgaa gttgctcaat actgtgcttc
    17161 tccggccata atgctgaacc gtctttctga atagcaggca aacacaacaa ttcccactct
    17221 tcaccgtttc cgccgtttaa taaccaaccg gctaaatcgt tctcgtgcag gcgttgcata
    17281 atcacgataa tcggcgtgtt ttccggactg ttcttacggc tctcaacggt attctgaaac
    17341 caatcaatca cattttgccg cattacttcg gagcgagcct catcagcttt atgcggatcg
    17401 tctatcagaa ttgctccgcc aaaaccggct cgctctttac ctgcaccaaa accggttatc
    17461 gtaccgcctg caccggtggc atagaacgcg ccgccttgtg aggttgtcca atggtttttt
    17521 gcttcactat caagtgtgac attcgggaag atttcttgat aggcttcgtg ttgaataagt
    17581 gagcggatat ttgaactatt attaaccgca agcgtagatg aataactagc gtgaataaac
    17641 tcgcagtccg gcacttgtcc aaagcaccac gccacgaaat tcacaatcgc aatttctgtc
    17701 tttgaataac gtggtggaat gttgatgatt aaccgcttac actcgccacg aaacactcgc
    17761 attaacgcgg cacaaatcaa ttcgtgatgt agtgcttgta gccattgata accccgcttt
    17821 tgctgaaaca tatagcgagt gaaaaaatag agatcggttc ttgccagttc agccgccacg
    17881 attttttgtg ctgcactaaa ctgaaacata actacacctt ttctaataat tctttggcaa
    17941 tcgccctaaa ttcatcgttg gataggttga tgttttggct ggtaacagtc cctgaaacct
    18001 cttgaatttg cttatcacgc catttatcag gctgcctatt tttgagccaa aaaatggctg
    18061 atgttggatc tggcggataa tgcttaatca aaggcgtttc aacaatttga ttatcaatca
    18121 ctcgaatatc aatatctgga gcctcatagc ccaatgctct tttataaagg ctgttggcta
    18181 cattcgcatc agctaataac tttccttttt ttatggactc caagaattca ggatattcaa
    18241 ttttccagtt attaatagta gattcagcca cttcaaaaaa atcggctata tctttgtccg
    18301 ttgcgccaag cagacataat ttttcaactt gagtaacgta ctctggttta tattttgtag
    18361 gttgaccacg tgacttagac gtggctttaa cctcgccttt tttagccatt gggttaaatt
    18421 cctttacttc attgcattta atagattagc aacagcatta atcagctttg gcgacacgaa
    18481 cgccaaaata gggactgtta ctgctaggct tatttgccat aaaccatatt ccataagaat
    18541 ctccttgatg gttacggtta aaaatgtaat aatttccata acttgttact cattccgttt
    18601 caatgggtaa taaaaaaaac ctcgaacacc gcaaatgttc ggggttttgt tttatataaa
    18661 tcacatcact taggtatttc ctcggtgttt tatccaacca accccgaaac catagctaaa
    18721 tcatgtagca tttacttttt attaaaacaa aagggagcaa ttaagctccc attctcatta
    18781 gcggcttaat ccgccaagtt atttaaaccc ttgcttagtc tgtgcttgcc a
//
LOCUS       DANJWC010000031        18660 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.031, whole genome shotgun sequence.
ACCESSION   DANJWC010000031 DANJWC010000000
VERSION     DANJWC010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18660
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.031"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            106..597
                     /locus_tag="PWX02_000798"
     CDS             106..597
                     /locus_tag="PWX02_000798"
                     /inference="COORDINATES: protein motif:HMM:NF019151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL1728831.1"
                     /translation="MYLTILEEIADKHYKKNKSDAPNFFENGQSTPHYIFNYLDYLLW
                     KNWDKYLDKKENIFIEKNQFRFSLSRTSIEHYLAQNRTSDSIVHNFGNLCLISPHQNS
                     ALSDYETTTKRSFYEGASKRFDCMSLKQAIMLSKENWTEKDIEEHCEDMKKLLDTKPS
                     QNK"
     gene            complement(740..922)
                     /locus_tag="PWX02_000799"
     CDS             complement(740..922)
                     /locus_tag="PWX02_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728832.1"
                     /translation="MYQFGYLRTKIGTDTQRAMLWNCPTYVLIIPFLLNTSVKKQYHA
                     FWQKYHQHNNDVSFPY"
     gene            1072..2112
                     /gene="selD"
                     /locus_tag="PWX02_000800"
     CDS             1072..2112
                     /gene="selD"
                     /locus_tag="PWX02_000800"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020909986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1117..1119,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDL1728833.1"
                     /translation="MSEPIKLTQFSHGAGUGCKISPKVLGTILQTQLEQFVDPNLLVG
                     NETADDAAVYDLGNGQAIISTTDFFMPIVDAPFDFGRIAAANAISDIYAMGGKPIMAI
                     AILGFPVNKLPAEVAQQIVEGGRFACQQAGIALAGGHSIDSPDPIFGLAVTGIVPTER
                     VKRNASAVAGCELFLTKPLGIGILTTAEKKGLLKPEHQHLASEVMCQINLIGSAFSEL
                     PEVTAMTDVTGFGLLGHLSEVCQGSGLRAEVDFEDIQVLDGTKEYIALGAVPGGTNRN
                     FDSYGHLISAMTDEQKAILCDPQTSGGLLVAVLPQAVEKVQQIANRANVPLFHVGRLL
                     EMEQGKPRIEVI"
     gene            2112..2585
                     /locus_tag="PWX02_000801"
     CDS             2112..2585
                     /locus_tag="PWX02_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL1728834.1"
                     /translation="MFKPNVTMACVVHCKGKFLFVEEIEYGKRTLNQPAGHLEKDETL
                     LEGASRELFEETGIRAEMANLVKIYQWQAPRSQTDFLRFLFSVELEEWLEPNPQDADI
                     TQALWLTLAEFEDYIQQQGQCARSPLVVQSVKDYLSGERYPLSLLNAFEWARNGK"
     gene            2575..3783
                     /gene="ubiH"
                     /locus_tag="PWX02_000802"
                     /gene_synonym="visB"
     CDS             2575..3783
                     /gene="ubiH"
                     /locus_tag="PWX02_000802"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HDL1728835.1"
                     /translation="MENKTTTQFDVVIVGGAVTGSVLALALSSFTHHKISIAIVEKSL
                     PDYEQQGGFDARSIALAEGSLQKMGQIRPLAGSSLGEMISEIGTPIQQIQVADKGHFG
                     KTTLKASEQNVAQLGMVVELAKFGRKLTACLAQQANITLFCPNQVVGIERSQQHCGLS
                     LKNGEKLSCSLLVAADGIQSQIAKQCGVETVKVRDYAQSAIIANVELSESHQNQAFEY
                     FTPQGPFALLPLSGNMMSLVWCVKEPNELIQLSDSEFLARLQQQFGWKLGQFVRVSKR
                     FVYPLILQKAQSHIHHRLAVVGNAAQMLHPVAGQGFNLGLRDLTTLAELVALAFNQGK
                     DIGEFSLLSTFENERTQDQARMINATSGLISLFCCEMLPVQVVRNLGLLTVSHSKIAR
                     DFVANQALGW"
     gene            4046..5278
                     /locus_tag="PWX02_000803"
     CDS             4046..5278
                     /locus_tag="PWX02_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HDL1728836.1"
                     /translation="MTTENMSTPKQAFIGLQMLFVAFGALVLVPLITGLDPNVALLTA
                     GVGTLIFQICTKRQVPVFLASSFAFIAPIQFGVQQWGIPVTMGALLCSGFIYMALSAL
                     VKAKGVNILHKYFPPIVIGPVIMVIGLGLAPVAVDMATGKSANIAYNTAIIVSMITLV
                     TTLIVAVFAKGLLKLVPILAGLTVGYILSVFMGLVDFTKVVEAPWFSLPKVSAPEFKL
                     DAILYLLPIALAPAVEHLGDVMAISQVAGKDFMKKPGLHRTLLGDGLATSAAAALGGP
                     PNTTYSEVTGAVMLTKNFNPKIMTWAACWAIAFSFCGKIGAFLATIPTFVMGGIMMLL
                     FGAVAVVGINTLIKAKVDLSIPRNLCIASVVMTFGIGGMLINIGEFSLKGISLCALVA
                     IVLNIVLPKEKVEDSAAH"
     gene            5345..6331
                     /locus_tag="PWX02_000804"
     CDS             5345..6331
                     /locus_tag="PWX02_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL1728837.1"
                     /translation="MQKLTFSPPLLNTPCPWCSELESLRELYACEYTGAVTTRTSMPQ
                     PYPHDWAKNQYVLFDSNAQKTASVNTQDATSLQTASLNTIGLSPNNLDTTISFVRTIS
                     NELTAPSSKAFIISVFGSPEEVGECYEKIVAFQTEVKMPLAMEINISCPNIPGEISPA
                     YSAEELSHYLHALQTSLKKLAQGDNALPIGIKIPPFTYQNQYDELIKGLLQAVENGEN
                     LPISFITSTNTLGSSLVLNAGKAVLNSEVGTGVGGMAGTAIHALSLGNVYTIRQMLDQ
                     EPKLKHLQIIGVGGVNDKAGFERMKSVGADFVGLATALGVEGIGVFKEILSE"
     gene            complement(6372..6782)
                     /locus_tag="PWX02_000805"
     CDS             complement(6372..6782)
                     /locus_tag="PWX02_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HDL1728838.1"
                     /translation="MLYSVLTAHILLNEPYRNGAHSTQHEHLVVMFEIDEESGYFYAM
                     DLHQTEQPVVDSLFVFRVSDIEQNSLKEPRRLEICWSEDGYQAFLLINGYPHAVFDFR
                     QFVGYNHTKFPMPELGSMWVHKETTNELVEKWLG"
     gene            complement(6848..8158)
                     /gene="eno"
                     /locus_tag="PWX02_000806"
     CDS             complement(6848..8158)
                     /gene="eno"
                     /locus_tag="PWX02_000806"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDL1728839.1"
                     /translation="MAKIVKVIGREIIDSRGNPTVEAEVHLEGGFVGLAAAPSGASTG
                     SREALELRDGDKGRFLGKGVLKAVSAVNNEIANALVGKDGSAQAEIDQIMIDLDGTEN
                     KSKFGANAILAVSLATAKAAAASKGLPLYAYIAELNGTPGVYSMPLPMMNIINGGEHA
                     DNNVDIQEFMIQPVGASTLKEALRIGAEVFHNLAKVLKAKGLNTAVGDEGGFAPNLAS
                     NADALACIKEAVEKAGYVLGKDVTLAMDCASSEFYDKERNVYDMKGEGKTFTSQEFTH
                     YLEGLCKEYPIVSIEDGQDESDWEGFAYQTKVLGDKVQLVGDDLFVTNTKILKNGIEK
                     GIANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQ
                     IKTGSMSRSDRVTKYNQLIRIEEALERAGTPAPFNGRKEIKGQA"
     gene            complement(8573..9292)
                     /locus_tag="PWX02_000807"
     CDS             complement(8573..9292)
                     /locus_tag="PWX02_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1728840.1"
                     /translation="MSKPNIYDESFSERMKWIAKNSFKDNYSEFARAVGVAQASLSRW
                     VKGEADPSRSNLVKIAEVSNVSLEWLATGKESKPQSENTSIVAREFERLEKMADDIVS
                     MINGFSTINVSAGFGSFNEGVTKPDGQEPYADSLLQSLGVKAHNCGVFWANGTSMHPT
                     IADGDQMLVDFSKREPRGDDKIYLVQNGESVWVKRIRKEWDYIELISDNESYRPIQIT
                     AEEAQNLQIIGQVVHIGHSLV"
     gene            9469..9747
                     /locus_tag="PWX02_000808"
     CDS             9469..9747
                     /locus_tag="PWX02_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1728841.1"
                     /translation="MSVLSDVKKTAQRDWHRADILAELRKKGWSLRTLADEGNVSYNT
                     LKSVLDKPYPKMERLVANAIGVAPEVIWAGRTAERIERNRRPTLTNKF"
     gene            9791..11626
                     /locus_tag="PWX02_000809"
                     /pseudo
     CDS             9791..11626
                     /locus_tag="PWX02_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            11674..12555
                     /locus_tag="PWX02_000810"
     CDS             11674..12555
                     /locus_tag="PWX02_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL1728842.1"
                     /translation="MKNQELRALMDSKGYQQKQVAQLLGVSVATVSLYLKGDYNGNVA
                     EMDRKVDELIERDKAKVVEAKYNAAFVPTLAARRGMEVMQFAHIEGEINVIFGAAGLG
                     KTQMLKEYERRNSSATLIEVDPSCTPKVLLRKIAEAVGANARGVNNELLESIVNKLKG
                     SERLLMIDEAELLSTRSLEFIRRIHDLTGVGVVLAGMPRLLVNLKGKNNELAQLYSRV
                     GFACDLGNALSEEDLGLLAESALGTNEFNAPLIKACKGNARRLSKLMRGVVRSSEINQ
                     TPISKDLVEQYSKMLIS"
     gene            12566..12814
                     /locus_tag="PWX02_000811"
     CDS             12566..12814
                     /locus_tag="PWX02_000811"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728843.1"
                     /translation="MTCFRHWTQRVTDGTEDLFWLDELLVGKDERDGSFTLSIFDPDI
                     DDFLLYEKVKTRKDWEIAYRKAREIINEQDIDCRSFRV"
     gene            12824..13144
                     /locus_tag="PWX02_000812"
     CDS             12824..13144
                     /locus_tag="PWX02_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728844.1"
                     /translation="MQKVFSEKAGEMMNPFNAYIHDSLVKLEIATLECEALDLEIERV
                     EFEAASPPRLVLRDNRKTQDFVKNGLASLFGMKTKGGRRYDSYQMQVRGVKCVWESER
                     VTKV"
     gene            13147..13338
                     /locus_tag="PWX02_000813"
     CDS             13147..13338
                     /locus_tag="PWX02_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728845.1"
                     /translation="MKKRRMIYALYVGEENVADGTAEELAKRLGVEAKTIRFMSSPAH
                     NKRVKYNGKAVRTVKVGLE"
     gene            13351..13968
                     /locus_tag="PWX02_000814"
     CDS             13351..13968
                     /locus_tag="PWX02_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3164 family protein"
                     /protein_id="HDL1728846.1"
                     /translation="MSKVTIGNEIYWKDADGNLKPEALVKEIDKERDELVRQFVSKAQ
                     ALSKALGDFKQSVFDDVGAFVSLSAEKYGVKIGGAKGNITLFTYDGEYKLQLAVQENI
                     RFDERIHAAKALIDECLHDWSEGAKPELKALIDNAFEVDKEGNLSTAKILSLRRVEID
                     DARWNQAMTAISDSVQVIGSKGYIRFYKRDENGKYQPISLDMAGV"
     gene            14287..14499
                     /locus_tag="PWX02_000815"
     CDS             14287..14499
                     /locus_tag="PWX02_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HDL1728847.1"
                     /translation="MKTEQKATKFDRFRYYAEKAAEAERKGNYIEAQDHWEVAKLSAK
                     STANLGWAEQRAEFCKRMHNKPFEGE"
     gene            14505..14687
                     /locus_tag="PWX02_000816"
     CDS             14505..14687
                     /locus_tag="PWX02_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728848.1"
                     /translation="MTEQDKNELNSQLNEALMQIIQAQKYLKQSDFIRSGVYLGTVQD
                     LLPKVHLKLLTANRKH"
     gene            14710..15285
                     /locus_tag="PWX02_000817"
     CDS             14710..15285
                     /locus_tag="PWX02_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5420 family protein"
                     /protein_id="HDL1728849.1"
                     /translation="MKFRFFKGDITQEPLKSIYADYQVSKEERKDKLNAILEKYPFND
                     GLISRSGWFHRIVIGIACKATNISQVLGVKGFKVSKFDDEFYLVKPDKRYKKGKELAE
                     DFKAINEIYEQHRDFSPYILKRLNMFYFASDFSYSPTSGRSYLAVADIFENTLLVKVP
                     MPIDKNDQPFPDIAEGLVEIKESEFLAIQGK"
     gene            15298..15666
                     /locus_tag="PWX02_000818"
     CDS             15298..15666
                     /locus_tag="PWX02_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728850.1"
                     /translation="MQQQIIKWLANGETGTSSKTMAFVIGFDVIPSRSGYPHDVSDFR
                     LCFQLVEAAPEMRNHIYKMKAISPIWSEFAENWEELERRYRAEQGQGRLPHTYALIQQ
                     ITDKDQNVIRMGNVSFSFGQ"
     gene            15738..15908
                     /locus_tag="PWX02_000819"
     CDS             15738..15908
                     /locus_tag="PWX02_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728851.1"
                     /translation="MEYQKMPEYRLCKKKSGDIVLQQLVICEITPATWHNPYPDSRTY
                     WVDLETIEEGAE"
     gene            15908..16462
                     /locus_tag="PWX02_000820"
     CDS             15908..16462
                     /locus_tag="PWX02_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728852.1"
                     /translation="MKLCKCPICHSEISLEALIEDDAGRELLNTIVNIGGNCGRAVVA
                     YLGLFKPQKNSLANSRALKIVQSLLELYTPSNVLEKALMDTVEQVRRNRRETGRCEPL
                     ANHNYLKKVYESVKPQFAVVRSEAKAVSSLEQQQRQADQKKHDAVMYVERMKQMGQLE
                     AVKNTESYQIWLKWQEEKQNATAN"
     gene            16446..16868
                     /locus_tag="PWX02_000821"
     CDS             16446..16868
                     /locus_tag="PWX02_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein GemA"
                     /protein_id="HDL1728853.1"
                     /translation="MQPQTRKQMIQKIHIGKNELKLDNNAYKMLLLEAVDKPSCSMMT
                     DSELMTVLQTMKAKGFVVKSKKYGKRPTASKTAPHRQGLINKIGALLYQSQKPWDYAH
                     AIAKRSFGIERVQWLTDEQLHKLVQMLASYNYRHGMSV"
     gene            17224..17823
                     /locus_tag="PWX02_000822"
     CDS             17224..17823
                     /locus_tag="PWX02_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HDL1728854.1"
                     /translation="MNNFQIATFTGSLQNQSTLLINARDLHQMLQIGRDFSNWIKSRI
                     ESYGFVENEDFTCSPNLASEKSKGLASFWGSQNKIDYHITLDMAKELCMLERSELGQQ
                     ARRYFIQKEKEANALKQAQPKATIEISTDKYIELLETQNQLLKQAMKPQKYKLRLSAE
                     EKSHIFRLHQQGLSTGQIVQQSGRSESAVRAVIRENLGK"
     gene            17834..18040
                     /locus_tag="PWX02_000823"
     CDS             17834..18040
                     /locus_tag="PWX02_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728855.1"
                     /translation="MRTEFEDIVQNTENLASGLYSLSHLLEILDNTEDQISMSGLAHL
                     VAIIASATKKQANDAATALNCVDN"
     gene            18133..>18660
                     /locus_tag="PWX02_000824"
     CDS             18133..>18660
                     /locus_tag="PWX02_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728856.1"
                     /translation="MFSFIKKLFDNKPSSIQNIEEPPKEIESELTSYDETIKTLFEKF
                     LVNYSIPERHNYNVRKRVSKEIIFSQLLESDSFSMPEDKRDRLYNALVISESVDYQEL
                     HAIFLLDDFKWRLFDNCREFCEENKIYPYAYQFLANKPAIPDNLESALLFVKVSQLRE
                     LLKDKLSQKIPTRKDE"
BASE COUNT         5712 a         3454 c         4313 g         5181 t
ORIGIN      
        1 catacttcgt tcaggtcaaa aatttataaa aattggcttt ttgatgtgtt gtataagatt
       61 agtgaacatc cgtccattaa aggtagttat aacttaaagg atgatgtgta tttaactatt
      121 ttagaggaaa tagctgataa acattataaa aagaataaat ctgatgctcc taattttttt
      181 gagaatggtc aatcaactcc tcactatatt tttaattatt tagattatct tttatggaaa
      241 aattgggata aatatctaga taagaaggag aatatattta tagaaaagaa tcagtttaga
      301 ttttctttgt cacgaacatc aatagaacat taccttgctc aaaatagaac aagtgattca
      361 atagttcaca attttggtaa tctttgtttg atttctccac atcagaattc agcattaagt
      421 gattatgaaa cgactacaaa gagaagtttt tatgagggag caagtaaacg tttcgattgt
      481 atgagtttaa aacaagctat tatgctttca aaagaaaact ggacagagaa ggatatagaa
      541 gaacattgcg aggatatgaa aaaactttta gatactaagc cttctcagaa taaataaatt
      601 ttcacaagcg gtgcaacttt gcaaaaactt cggtattatg tagcagttgc acaaaacccc
      661 aaattcatta aaaccgctta aaaatgaaat gccctaaact gatttttcag tctagggcat
      721 ttattatcac atattatggt taatagggaa aactcacatc attgttatgc tggtgatatt
      781 tttgccaaaa tgcgtgatat tgttttttca ccgatgtgtt taataaaaac gggattatca
      841 agacataggt cgggcaattc catagcattg cacgttgtgt atccgtgccg attttggtgc
      901 gtaagtaacc aaactgatac attagaccca atccaaccgt cacccattct aatagcataa
      961 taactaaggt cgcatcatta aattaaaagc tcattttttg caaaaaattt cccaaattcc
     1021 accgcttgtt ggcgtacaat aggcataatt ttgatttacg atggaggcag aatgtctgaa
     1081 ccgataaaac ttacgcagtt cagccacggg gcgggttgag gctgtaaaat ttcgcctaag
     1141 gtgttaggga caattttaca aactcaatta gaacaatttg tcgatccgaa tttattggta
     1201 ggcaatgaaa cggcggacga tgctgccgtc tatgatttag ggaacgggca ggccattatc
     1261 agcaccaccg atttctttat gccgattgtg gacgccccgt ttgatttcgg gcgaattgct
     1321 gctgccaatg cgattagcga tatttatgcg atgggtggca agccgattat ggcgattgcg
     1381 attctgggct ttccggtgaa taaattgccg gctgaagtgg ctcaacaaat tgtcgaaggt
     1441 gggcgttttg cgtgccagca agccggtatt gccttggcgg gcgggcattc gattgattcg
     1501 cccgatccga ttttcggctt ggctgttacc ggcattgtgc cgaccgaacg ggtgaaacgc
     1561 aatgcctctg cggtggctgg gtgtgagctg tttttaacca agcctttggg gattggcatt
     1621 ctaaccacgg cagagaaaaa aggtttgttg aagcctgaac atcaacatct ggcaagcgag
     1681 gtaatgtgcc aaatcaactt gattggttca gcgttttccg aactgcctga agtcacggca
     1741 atgactgatg taaccggttt tggcttactc gggcatttaa gcgaggtgtg tcaaggctcg
     1801 ggcttacggg cggaggttga ttttgaggat attcaagtcc ttgatggcac taaggagtat
     1861 attgcattgg gagcagttcc gggtgggaca aatcgcaatt tcgacagcta tgggcatttg
     1921 atttcggcaa tgacggacga gcaaaaagcg attttgtgcg atccgcaaac ctctggcggt
     1981 ttattagtgg cggtgctgcc acaagcggtc gaaaaagtgc aacaaattgc aaatcgggca
     2041 aatgtgccgt tattccacgt tggcagattg ctggaaatgg aacagggcaa accacggatt
     2101 gaggtaattt gatgtttaaa ccaaatgtaa caatggcttg cgtggtgcat tgtaagggaa
     2161 agtttctgtt tgtggaagaa attgaatatg gcaaacgtac ccttaaccag ccggcagggc
     2221 atttagaaaa agatgaaacc ttgctggagg gggcaagccg tgagctgttt gaggaaaccg
     2281 gtattcgggc agaaatggca aatttggtga aaatttatca atggcaagcc cctcgttcac
     2341 aaaccgattt cttgcgtttt ctgttttcgg tggaactgga agagtggctg gagccgaatc
     2401 cgcaagatgc ggatattacc caagcccttt ggcttacttt agcagaattt gaagactaca
     2461 ttcagcaaca agggcaatgt gccagaagcc cgttggtggt tcaatcggtc aaagattatt
     2521 taagcggtga gcgttatcca ttaagtttgt tgaatgcgtt tgaatgggcg agaaatggaa
     2581 aataaaacaa caacccaatt tgatgtggtg attgtgggcg gggcagtgac cggctcggtg
     2641 ctggcattgg cgttaagtag ttttactcat cacaaaatct cgattgcgat agtggaaaaa
     2701 tcgctgccgg actatgaaca gcaaggcggt tttgatgctc gcagtattgc cttagccgaa
     2761 ggcagtttgc aaaaaatggg gcaaatcaga ccgcttgcag gctcaagttt aggcgagatg
     2821 attagcgaaa tcggcacgcc gattcagcaa attcaagtag cggataaagg gcattttggt
     2881 aaaacgacct tgaaagcaag cgagcagaat gtggctcaac tgggtatggt ggtggaactt
     2941 gcaaaattcg gcagaaaatt gaccgcttgt cttgcacagc aagctaatat tacgcttttt
     3001 tgcccgaatc aagtggttgg cattgagcgt tcccaacaac attgtggatt gagcttgaaa
     3061 aatggtgaga aactaagctg ttccttgctg gtggcggcag atggtattca atctcagatt
     3121 gcaaaacagt gcggtgtgga aaccgtaaaa gtaagggatt atgcacaatc ggcaattatc
     3181 gccaatgtgg aactgagtga atctcaccaa aatcaagcct ttgaatattt tacgcctcaa
     3241 ggcccttttg ctttattgcc tttgagcgga aatatgatgt cgttggtgtg gtgtgtgaaa
     3301 gagccgaacg agttaatcca actttcagac agtgaatttt tagcccgttt gcaacaacaa
     3361 tttggttgga agttagggca atttgttcga gtgagcaagc ggtttgttta tccgctcatt
     3421 ttgcaaaaag cccagtcgca tattcaccac cgcttagcag ttgtcggcaa tgccgcacaa
     3481 atgcttcacc cggttgctgg gcaaggtttc aacttaggct tgcgtgattt gactacctta
     3541 gcggaattag tggctttggc gtttaatcaa gggaaagata taggcgaatt ttcgctctta
     3601 tcaacttttg agaatgagag aacgcaagat caagccagaa tgataaatgc tacaagcggt
     3661 ctgatttcgc ttttttgttg cgaaatgtta ccagttcagg ttgtgaggaa tttagggtta
     3721 ttaacggttt cccattcaaa aatagccaga gattttgttg ctaatcaggc attaggttgg
     3781 taaaaaacac aaaagcagca attttctatt tgacactgtt tttaaaaagg cgtattatcc
     3841 agcccgttta aaaatctcac caaagccttg aatgcaaatt caaggctttt tcctttttag
     3901 tgaaagccta tgaattttgt ttgccgtaaa atcggcaaaa aaattttgcc taaaattcgc
     3961 aatcgtttgc gaaataggtt tatagcgttg ttttcatctg ggtaactgca atggttacat
     4021 cgcaatacat caaagagagt aaaaaatgac cacagaaaat atgagtacgc caaagcaggc
     4081 gtttatcggg ctgcaaatgc tgtttgttgc atttggggcg ttagtattag ttccgctgat
     4141 taccggttta gatcctaatg ttgcccttct taccgccggt gtcggtacgt taatcttcca
     4201 aatttgtact aaacgtcaag ttcctgtttt cctcgcatca agtttcgctt ttatcgctcc
     4261 aatccaattc ggtgttcaac aatggggaat tcccgtcaca atgggagcat tgctctgttc
     4321 cggttttatt tatatggcat tatctgcttt agtgaaagcg aaaggggtga atattctgca
     4381 taaatacttc cctccaatcg tgattggacc ggtaattatg gtgattggtt taggtcttgc
     4441 tcctgttgcg gtggatatgg caacgggtaa atcggcgaat attgcctata acaccgctat
     4501 tattgtttca atgataacgc tggtaactac gctgattgtg gcggtgtttg cgaaagggct
     4561 attaaagctc gtgccaatat tagcaggttt aacggtggga tatatcttgt ctgtttttat
     4621 gggattggtg gattttacta aagtggttga agctccttgg tttagtctac cgaaagtttc
     4681 cgcaccggaa ttcaaattag atgcaatctt gtatttatta cctatcgcac ttgctccggc
     4741 agtcgaacac ttaggcgatg taatggcaat cagccaagtt gccggtaaag attttatgaa
     4801 aaaaccgggg ttacaccgca cgcttttagg tgatggttta gcaacttcag cagctgcggc
     4861 attaggtggc ccaccaaata cgacttactc tgaagtaacc ggtgcggtaa tgctaaccaa
     4921 aaacttcaat ccgaaaatta tgacttgggc tgcgtgctgg gctatcgctt tctcattctg
     4981 cggaaaaatc ggggcattcc ttgccactat ccctactttt gtgatgggcg gtattatgat
     5041 gttgctgttc ggtgcagttg cggttgtggg gatcaatact ctaattaaag ctaaagttga
     5101 tttaagcatt ccacgtaatc tttgtattgc ttctgtagta atgacattcg gtattggcgg
     5161 tatgttaatt aacatcggcg aattctcatt aaaagggatc agcttgtgtg cattagtcgc
     5221 tattgtactt aacattgtat taccaaaaga aaaagtagaa gattctgctg ctcattaatc
     5281 aaaggacgct gtggcgtcct ttttgctaat aaccctctat ttgtaaataa aaaggaataa
     5341 cactatgcaa aaactcacat tttcaccacc acttttaaat acgccttgcc cttggtgttc
     5401 ggagttagag agcttgcgtg aactttatgc gtgtgaatat accggtgcgg taacaacccg
     5461 tacttcaatg cctcaaccat atccacacga ttgggcgaag aaccaatatg tactctttga
     5521 ttccaacgca cagaaaacag caagtgtgaa tactcaagat gcaactagct tacaaacagc
     5581 aagccttaac accatcggtt taagcccaaa taatctggat accaccatca gctttgtacg
     5641 caccatttca aatgagctga cagcgccatc aagcaaagca tttattattt cggtgtttgg
     5701 ttcacccgaa gaggtggggg aatgttatga aaaaattgtt gctttccaaa ctgaagtgaa
     5761 aatgcctttg gcaatggaga ttaatatctc ttgcccgaat attccgggtg aaatttcgcc
     5821 tgcttattcc gcagaagaat tatcacatta cttgcacgca ctacaaactt cgctaaaaaa
     5881 actggctcaa ggtgataatg ctttaccaat cgggattaaa attccacctt ttacttacca
     5941 aaatcaatat gatgaattaa tcaaaggctt attacaagcg gtcgaaaatg gcgaaaattt
     6001 accaatttcc tttattactt caaccaatac gcttggctca tcgcttgtct taaatgctgg
     6061 caaagcggta ttaaattcag aagtgggaac gggcgtaggc ggaatggcag gaacggcaat
     6121 ccatgcactt tctttgggga atgtttacac gattcgccaa atgttagatc aagagcctaa
     6181 gctcaaacat cttcaaatta ttggtgtggg cggcgtgaat gataaagccg gctttgagcg
     6241 aatgaaatcg gttggggcag actttgtggg gttagctacc gcattagggg tggaaggaat
     6301 cggcgttttt aaagagattc tgtctgaata atgaagagga gggcgtgcat tgcacgcccg
     6361 aataaatgaa attagcctaa ccatttctcc actaattcat tagtggtttc tttatgtacc
     6421 cacatactgc caagttccgg cattgggaat ttggtgtggt tgtagccgac aaattggcgg
     6481 aaatcaaaca cggcgtgcgg gtagccgttg atcagtaaaa aggcttgata gccgtcttca
     6541 ctccagcaaa tttctaaacg gcgaggttct tttaatgaat tttgctcaat atcgctgacc
     6601 cgaaacacaa ataaactatc cactaccggc tgttcggttt ggtgtaagtc cattgcataa
     6661 aagtagccgc tttcttcatc aatttcaaac atcactacta aatgttcgtg ctgggttgaa
     6721 tgagcgccat tacggtaagg ttcgttcagt agaatatgag cagttaaaac agagtagagc
     6781 attaaattat ccttataagt aaaatgccac cgatttctca gtggcattta aatatttaat
     6841 caggcaatta cgcttgacct ttaatctctt tacggccgtt gaacggagcc ggtgtaccag
     6901 cacgctctaa cgcttcttcg atacggatta actggttgta tttcgttaca cggtctgaac
     6961 ggctcattga accggttttg atttgacctg ctgctgtacc tactgctaaa tctgcaatgg
     7021 ttgcatcttc agtttcgcct gaacggtgag agataactgc ggtgtaacct gcatctttcg
     7081 ccattttgat tgcagctaaa gtttcagtta aagaaccgat ttggttgaat ttgattaaga
     7141 ttgagtttgc gatacctttc tcaataccgt ttttcaagat tttggtattg gttacgaata
     7201 agtcatcacc aactaattgt actttgtcgc ctaatacttt agtttggtat gcgaaacctt
     7261 cccagtcaga ttcgtcttga ccatcttcga tagacacgat tgggtactct ttgcataagc
     7321 cttctaagta gtgagtgaac tcttgtgaag taaaggtttt gccttcgcct ttcatatcgt
     7381 acacattgcg ttctttgtcg tagaattcag atgatgcaca gtccatcgct aaagttacgt
     7441 ctttacctaa cacatagcct gctttttcta ccgcttcttt aatgcacgct aatgcgtctg
     7501 cgtttgaagc caagtttggt gcgaaaccac cttcgtcacc tacagcagta tttaagcctt
     7561 tcgcttttaa tacttttgca agattgtgga atacttcagc accgatacga agagcttctt
     7621 ttaatgtaga agcacctacc ggttgaatca taaattcttg aatgtccacg ttgttgtcag
     7681 cgtgttcacc accattgatg atgttcatca ttggtaatgg cattgagtaa acacccggtg
     7741 tgccgtttaa ttcagcaatg tatgcgtaaa gcggtaagcc ttttgaagcg gctgctgctt
     7801 ttgctgttgc taaagaaacc gctaagatag cgtttgcacc gaattttgat ttgttttctg
     7861 tgccatctaa atcgatcata atttggtcga tttcagcttg tgcagaaccg tctttgccta
     7921 ctaatgcgtt tgcaatttcg ttatttacgg cagaaaccgc ttttaataca cctttaccta
     7981 agaaacgacc tttgtcgcca tcacgtaatt ctaatgcttc acgtgaaccg gtagaagcac
     8041 ctgatggagc ggctgctaag cctacaaaac caccttctaa gtgaacttct gcttctacag
     8101 ttgggttacc acgagagtcg atgatttcac gaccgattac tttaacaatt ttagccattg
     8161 ttgatgtcct ctaatatacg agttaataga aaaaagaata ggtatatagt aaagcgattt
     8221 tctgcttttg tcttggaaaa actgattgag tggcagaaaa tttgccacta atttgatcta
     8281 agactatatt cgcctgagat tatagcaacc tttgcaaaaa acggcaaaaa tttgcccgct
     8341 tgtatgctta ttttttgatt aaatgtgggg tactgatctt ttttgcatag agagaaagaa
     8401 aaaaatttgg gcggaaaaat aacggcgagg ctaggaaata caaggcttcg ccgtttttgt
     8461 ttggttgggg ctatgcaaaa aaacaaaatg agcaggatta gaaaaaaatg agcagttttt
     8521 gcgatatttt ttgcataggt ttgatcggtt ttaaaaccat tttaaagcag ttttaaacga
     8581 gagaatgccc gatatgtacc acttgtccga tgatttggag gttttgagct tcttcggcgg
     8641 taatctgtat tgggcggtag gactcgttat cgctaattaa ttcaatatag tcccattctt
     8701 ttctaatccg tttaacccat acgctttccc cattctgcac aagataaatc ttatcatcac
     8761 cacgaggctc tcgtttgctg aaatctacca gcatttggtc accgtctgca atggtagggt
     8821 gcattgatgt tccatttgcc caaaacacgc cgcagttgtg ggcttttacg ccgaggcttt
     8881 gcagcaggct gtcggcgtaa ggttcttgcc cgtctggctt cgttacgccc tcgttaaagc
     8941 tgccaaagcc tgctgaaacg ttgattgtgc tgaatccgtt aatcattgaa acaatatcat
     9001 cagccatttt ttctagtcgt tcaaactctc ttgccacaat actggtattc tctgattgtg
     9061 gtttgctttc cttacctgtt gccagccatt caaggctcac atttgacacc tctgctattt
     9121 ttacaagatt tgaacgagat ggatctgcct ctccttttac ccacctagat agtgatgctt
     9181 gggctacccc cactgctctt gcaaactcac tgtaattgtc cttaaagcta ttttttgcaa
     9241 tccatttcat tctttctgag aagctctcat cgtaaatgtt tggtttactc atcttttacg
     9301 tttccattaa taaagtttta ctcgtaaaac atactcataa accattgatt ttcaagtgat
     9361 ttgtgaataa agcaaaaata ttttaaatat ttttatgttt tacggttgat ttatttaaag
     9421 ttttacggtt aaatacacac atcagataga taaaaagagg tgtatcaaat gagtgtatta
     9481 agcgacgtta aaaaaaccgc acagagagac tggcatcgag ccgatattct ggcggaactt
     9541 cgtaaaaaag gttggtcact tcgcactttg gcagatgaag gtaatgttag ctataacacc
     9601 ctgaaatcag tcttagacaa gccttatccc aaaatggaac gtctagtcgc aaatgctata
     9661 ggcgttgctc ctgaagtaat ttgggcagga cgaaccgcag aacgtataga acgtaatcgg
     9721 cgaccaacct taacgaataa gttctaatca taaatgatct ttaacgtaaa agatacaaaa
     9781 aggagcattt atgagtgaga taagtttaaa aacacactat tcagtagctg aattattaag
     9841 ttttaagtta tctagtttgc caagtgctca taaaaatgtg cttgataaag ctataagaga
     9901 aaactggcaa tctcaaaaac gcaaagggcg tggcggtggt gttgagtatg aattagcctg
     9961 cctccctcaa gaaatccaaa ccgaaatccg ctcccgcttt atgtcggcgg tggtggaagc
    10021 caagccgaaa aagttaccgg tagtgcgtgc ggaggtggag ttaggcaacc ttactaccaa
    10081 acagcgagaa attgctgatg cacgaatggc actggtggcg tatgtgttgg agttggaagg
    10141 ctcaatgagc cgaattaaag cgataaccta cctttgcaac cttgccaaac aaggcgaaat
    10201 gccaccgcac ttggcggagc tggttgccgt agcaaatgct aaaaagacgg caaaacgcac
    10261 actctcggtg cgaaccttaa acggctgggt ggtggattac tgcaaggcgg agaacgccga
    10321 gcaacggttg aaactgcttg ccccacaggt acggcaggag accaagccag aagaaatttg
    10381 gtggctgagt gcctttttgg ggacgtatcg tcagaaaaac ggcatttgcc tcaccgaagc
    10441 ctaccgagaa tttgaggtgg aatgggcgta tcagtatgca gacaacccgc tactgttggc
    10501 acaatgcccc agcctaagcc aagtacagcg agcgatgaat aagttgccgt tgtatgtgaa
    10561 agagtacggc agacgcaccg gctcgcatta caagcagttg cttagctatg tgaagcggga
    10621 ttggtcggta cttcgagcaa atgatgtgtg gattggtgac ggtcactcgc tcaaactcaa
    10681 agtggcccac ccgatacacg gcaagccgtt tacccctgag ctgacaatga ttgttgatgg
    10741 tgcaggcaga aaggtggtcg gttggtcgct ggcactcagc gagaatgctt ttgcagttgc
    10801 tgatgcccta aggcacgcca ttagcctaca cggtgtgccg gcaatctact actcggataa
    10861 cggtggcggt gagaaaaaca aatttttgga tgcagaggtg acggggatgt tgccccgcct
    10921 cggtatccga cacgaaacag ggattgcagg caatccgcag gggcgaggca ttatcgaacg
    10981 gttaaataaa accatcggta tgacgattgc ccgacagttt gaaaccggtt acgcaagcgg
    11041 tgcagatccg gaaacagtgc gtaaaaacct gtatgcggtg aactcgctgg caaatgccaa
    11101 aggggaatta accccactac aacgtaaagc acaaggcaaa ttgccaacgt ggcagcagtt
    11161 gattgatgtg atccagtctg tgattgattg gtacaacaac gagcatattc actcggaaat
    11221 ccgtaccacg ccggcacgca aatatcagca gatgttgcac gcagaagatg tggtgatgtt
    11281 aagccctgtt gagttgcggg atatgagccg accggaattt atccgtaagc cggagcgagg
    11341 ttgggtaagt tggaataaca accactattt taacctgaaa ttactggatt ttgaccgtga
    11401 agaagtggtg atcggggttg atattcacaa tgccgagagc gtgcaggtgc gtaccaaaga
    11461 cggcagattt atttgcgagg caatttggaa cggcaacacc cgagaggcat tcccggtggc
    11521 gatggtggaa cagcagcgta aagaacgcca taaacgccga tccaacctta aacagcaaca
    11581 gcttgatgag attaatgcgg aattaaaccc ggttttaacc attgagcaga aaaagaggat
    11641 tacgaaaaaa agttagcagt gggaggctaa acgatgaaaa accaagaact tagagcctta
    11701 atggatagca aaggctatca acaaaaacaa gtggcacagc ttttaggtgt atccgttgcc
    11761 acagtcagtc tgtatctcaa aggcgattat aacggcaatg tggctgaaat ggatcgcaaa
    11821 gtagacgagt tgattgagcg ggataaggcg aaggtggtgg aagcgaaata taacgctgca
    11881 tttgtgccaa ctttagcggc acgcagaggg atggaggtaa tgcagtttgc ccatattgaa
    11941 ggcgagatta atgtgatttt tggggcagcc ggtttaggta aaacccaaat gctcaaagaa
    12001 tacgaacgcc gaaacagctc tgccacatta attgaggttg atccgagttg cacacctaaa
    12061 gtcttgctcc gcaagattgc cgaggcggtg ggggcaaatg ccagaggggt aaataacgaa
    12121 ctattagaaa gcattgtgaa caagttaaaa ggctctgaaa ggttgctaat gattgatgaa
    12181 gctgaattac tttcgacccg ctcgctagag tttatccgcc gaatccacga tttaaccggc
    12241 gtgggcgtag tgttagccgg tatgccgaga ttgttggtta atcttaaagg taaaaataat
    12301 gagctggcac agctttatag ccgagtaggt tttgcttgtg acttaggcaa tgccttgagc
    12361 gaggaagatt tagggctgtt agcagaaagt gcgttaggta caaatgaatt taatgccccg
    12421 ctgattaaag cctgcaaagg taacgcacgc cgattaagta agttaatgcg tggtgtcgta
    12481 cggtctagcg agattaacca aacgccaatt agtaaagatt tggttgagca atatagcaag
    12541 atgttgatta gttagggagc gaattatgac ttgttttaga cactggacac aaagagtaac
    12601 agatggaaca gaagatttgt tttggttgga tgagttgcta gttggtaaag atgagagaga
    12661 tggtagtttt acactttcta tctttgatcc tgatatagat gactttttgt tatatgagaa
    12721 agtaaaaaca agaaaagatt gggaaatagc ttatcgaaaa gccagagaaa ttattaatga
    12781 acaggacata gattgtagaa gctttagagt ttaaggggaa agtatgcaaa aggtatttag
    12841 cgaaaaagca ggcgaaatga tgaacccatt taacgcttat atccacgaca gccttgtgaa
    12901 gttagaaatt gccacgcttg agtgcgaggc gttggatttg gaaattgagc gggtggaatt
    12961 tgaggcggca agcccacctc gccttgtgtt gcgagataac cgcaaaacgc aggattttgt
    13021 gaaaaacggc ttagcaagcc tatttggaat gaaaacaaaa ggcggcagac ggtatgacag
    13081 ctaccaaatg caggtgcggg gtgtgaaatg cgtgtgggag tctgaacgtg ttacgaaggt
    13141 gtagaaatga aaaaacggcg aatgatttat gccctgtatg tcggcgaaga gaatgtggct
    13201 gacggcacgg cggaagaatt agcaaaacgg ttgggagtgg aagcaaagac gatccgattt
    13261 atgtcgtccc ctgctcataa caaacgagtg aagtataacg gtaaagcagt ccgcaccgtg
    13321 aaagtcgggt tggaatagag aggaagtatt atgagtaaag taacaatcgg taatgaaatt
    13381 tactggaaag atgctgatgg caatttaaag ccggaggcat tagtaaaaga aatcgacaaa
    13441 gaacgtgatg aattagttcg ccaatttgta agtaaagcac aagcgttaag caaagcgtta
    13501 ggcgatttta aacaatccgt atttgatgac gtaggggctt ttgtcagcct atcggctgaa
    13561 aaatatggag taaaaattgg cggtgcaaaa ggcaatatca cactatttac ttatgacggt
    13621 gaatataagt tgcagttagc agtgcaagag aatattcgct ttgatgagcg tattcacgca
    13681 gcaaaagcct tgattgatga atgcttgcac gattggtcgg aaggggcaaa accggagctt
    13741 aaagccttaa ttgataatgc gtttgaggtg gataaggaag gcaatttatc tacggcgaaa
    13801 atcctatccc tacgccgtgt tgagattgat gatgcacgct ggaatcaagc aatgacggca
    13861 atttcagaca gcgtgcaggt aattggtagt aaaggttata tccgttttta taaacgtgat
    13921 gaaaatggca aatatcagcc gattagttta gatatggcgg gggtttagaa tgtttattac
    13981 ttttaatgtt aattatacgg ataaacctgt tgtggttaat acagacaagg tttgttcaat
    14041 agaaaatatt aacgggaatg taacagtaca cttttgtgat aacacaaaat taataatgat
    14101 ggactttgat gataaagaat atgtatcgtt attaaatcat ttgcatattt taaatcagtt
    14161 aaaaagaaag tcttagttaa aaaacctttt caatgccctt taaaaatctc ccctagcccc
    14221 tctttttcaa agagggggac aagatagaag ggcatttata aagtgtttta accacaggag
    14281 gaaaaaatga aaacagaaca aaaggcgact aagtttgacc gctttcggta ctatgccgag
    14341 aaagccgcag aagcagaacg caaaggcaat tatatagaag cccaagacca ctgggaagtt
    14401 gcaaaattat cggcaaaatc gaccgctaac cttggctggg cggaacaacg ggcggaattt
    14461 tgtaaacgta tgcacaacaa gccatttgaa ggggaataaa tatgatgacc gaacaagata
    14521 aaaacgaact taacagccaa ttaaacgagg cgttaatgca gattattcaa gcccaaaagt
    14581 atttaaaaca aagtgatttt attcgcagcg gcgtgtatct cggcacggtg caagatttgt
    14641 tgccaaaggt gcatttgaag ttattaacgg caaatcgtaa acattaacta aactagcaag
    14701 gagttaaaaa tgaaatttag attttttaaa ggggatataa cccaagagcc actaaaaagc
    14761 atttatgcag attatcaagt atctaaagaa gaacgcaagg ataagctcaa tgctatttta
    14821 gaaaaatatc ctttcaatga tggattaatt agtaggtcag gctggtttca tagaatagtt
    14881 atagggattg cttgtaaagc aaccaatatt agccaagttt taggtgttaa agggttcaag
    14941 gtcagtaaat ttgatgatga gttttacctc gtcaaacctg ataaacgtta caaaaaaggg
    15001 aaagaacttg cagaagattt taaagcgatt aatgaaattt atgaacaaca tcgtgatttc
    15061 tcaccctaca tcttaaagcg tttgaatatg ttttattttg caagtgattt tagttattcc
    15121 ccaacatcag gtcgaagtta tttagccgta gcagacattt ttgaaaacac cttactggtt
    15181 aaagtgccaa tgcctattga taaaaatgat caaccatttc ctgatattgc agagggattg
    15241 gttgagatta aagaaagtga gtttttagca atacagggga aataaaaaat gacagaaatg
    15301 caacaacaaa tcattaaatg gctagcaaat ggtgaaacag ggacaagtag caaaacaatg
    15361 gcatttgtaa ttggttttga tgttatccca agtcgtagcg gttaccctca cgacgtgtct
    15421 gatttccgcc tttgttttca gttggtagaa gccgctccag aaatgcgtaa ccatatttac
    15481 aagatgaagg caatcagccc aatttggtcg gaatttgccg aaaattggga agagttagaa
    15541 agaagatata gagcggagca aggtcaaggg aggttaccgc acacctatgc cttaattcaa
    15601 cagattactg ataaagatca gaatgtcata agaatgggga atgtgagttt ttcatttggt
    15661 caataagcaa aacccattta cagcccattt aacccctatt taagtgggct gaataatgtg
    15721 ttttatagga gcataaaatg gaatatcaaa aaatgcctga atatcggtta tgtaaaaaga
    15781 aatcgggaga tattgtgtta caacaattag ttatttgcga gataactcca gcaacttggc
    15841 ataacccata ccctgatagt cgaacttatt gggttgatct cgaaacaata gaagaaggag
    15901 cagaatgatg aaactatgca aatgcccgat ttgccattcg gaaatctcgc tggaggcgtt
    15961 gattgaagat gatgcggggc gtgagttgtt aaatacgata gtcaatatcg gtgggaattg
    16021 tgggcgtgca gtggtggctt atttagggtt atttaagcca cagaaaaaca gccttgccaa
    16081 tagccgagcc ttgaaaattg tgcagagttt gttggagctt tatacaccct ctaatgtgtt
    16141 agaaaaagcc ttaatggata cggtggagca agtccgccga aatcgtagag aaaccggtag
    16201 atgtgagccg ctggcaaatc ataattactt gaagaaagta tatgaaagcg tgaagccaca
    16261 atttgcagta gtaagaagtg aagcgaaagc agtgagtagc cttgagcaac agcaacgcca
    16321 agcagaccaa aagaaacacg atgcggtgat gtatgtggaa cgaatgaagc aaatggggca
    16381 actggaagcc gttaagaaca ccgaaagtta tcaaatttgg ctgaaatggc aggaggaaaa
    16441 gcaaaatgca accgcaaact agaaagcaga tgatacagaa aatccatatc ggtaaaaatg
    16501 agctaaaact ggataacaac gcctacaaga tgctattgct ggaagcggtg gataagccga
    16561 gctgctcaat gatgaccgac agcgaattaa tgaccgtatt gcaaacaatg aaagccaaag
    16621 ggtttgtggt gaagtcgaaa aaatacggca aacgcccaac agcaagtaaa accgcccctc
    16681 accggcaagg gttgataaat aaaatcgggg cgttgcttta tcaaagccaa aagccgtggg
    16741 attacgccca cgccattgcc aaacgctcgt ttggtattga gcgggtgcaa tggctgaccg
    16801 atgagcagtt gcataaatta gtgcagatgt tggcgagtta taactacagg cacggaatga
    16861 gtgtatagaa tttatttaca gccttctgcg gagggctgaa taataaattt tacgaaatat
    16921 tttgcatagt tatgattttt gggcttgact gtcaagcgga aaaagtttat ttttcgacaa
    16981 cgatctcaaa aatcgacaat acagcttgca cggctgaaag tatgtgctat ttttttgcct
    17041 gaatatcaga aagcctttga gcttcggaat atccgtgtag aaaaataaat ataatttaaa
    17101 tcaatgaccg acagtgcgag gaatacaaga cccgaaaggg gaataactcc gctgtttatt
    17161 gtgcagtttt gagctgtcgg tcgccctact caaaataggg tttccaattc aaggaaaaac
    17221 acaatgaata acttccaaat tgcaaccttt acgggttctc ttcaaaatca atctacctta
    17281 ctaattaatg cacgtgatct acatcagatg ttacagattg ggcgtgattt ttctaactgg
    17341 attaaatcaa gaattgaaag ctacggcttt gttgaaaatg aagattttac ttgctcgcca
    17401 aatttggcga gcgagaaaag caaaggttta gccagctttt ggggcagtca aaataaaatt
    17461 gactatcaca tcaccctcga tatggcaaaa gaactctgta tgcttgaacg ctccgaattg
    17521 gggcaacagg cacgccgtta ctttatccaa aaggagaaag aagccaacgc cctgaaacaa
    17581 gcccaaccca aagccacaat cgaaatcagc accgataaat acattgagct actcgaaacc
    17641 caaaaccaac tgcttaaaca agcgatgaaa ccgcaaaagt acaagctacg cctaagtgcc
    17701 gaagaaaaaa gccatatttt ccgcttgcac cagcagggat taagcacagg gcaaattgtg
    17761 caacaatcgg ggcgttcaga aagtgccgtg cgtgcggtta ttcgtgaaaa tttaggcaaa
    17821 taaggagtgg gttatgagaa cagaatttga agatattgtg caaaataccg aaaatttggc
    17881 aagtggcttg tatagtttaa gccatttact ggaaattttg gataataccg aagaccaaat
    17941 ttcaatgagt ggcttagccc atcttgtcgc aatcattgcc agtgcgacta aaaaacaagc
    18001 aaatgatgca gcaacagcct taaattgtgt cgataattaa aaatttacac tattttttgc
    18061 atagcccact ttttaaagtg ggcttttttg tggtagattt taaacaattc aggcagagaa
    18121 ttgagggtta agatgttctc atttattaaa aaattatttg ataataaacc atcatctata
    18181 caaaatattg aagagccacc taaggaaata gaatcagaat tgacatcata cgatgagacc
    18241 ataaaaactc tttttgagaa atttttagtc aattattcta ttcctgaaag acataattac
    18301 aatgtcagaa agcgagtaag taaagaaatt atcttctctc aattattaga atctgatagt
    18361 tttagtatgc cagaagataa aagagatagg ctatataatg ccctagttat ttcagagagc
    18421 gtagattatc aagaattaca tgctattttt ttgctagatg atttcaaatg gaggctattt
    18481 gataattgca gagagttttg tgaagagaat aaaatttatc catatgcata tcaattttta
    18541 gcaaataaac cagctatccc tgataattta gaaagtgcgt tactttttgt aaaagtttca
    18601 caacttagag agttgttaaa agataaatta tcccaaaaaa tccctacaag aaaagatgaa
//
LOCUS       DANJWC010000032        18530 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.032, whole genome shotgun sequence.
ACCESSION   DANJWC010000032 DANJWC010000000
VERSION     DANJWC010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18530
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.032"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..558)
                     /gene="cas4"
                     /locus_tag="PWX02_000825"
     CDS             complement(<1..558)
                     /gene="cas4"
                     /locus_tag="PWX02_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HDL1728857.1"
                     /translation="MDDLQNSTEILPLVQPKFGYDKRSISLSALQHYAFCPRQCALIH
                     NEQVWAENYLTAQGQVLHERVDSGEPETRKGIRFERTVHVAAEKLGISGILDLVERDL
                     KTGELKPVEYKRGKPKPEPMDEIQLCAQALCLEEMTGQTINEGALWYMQTRHRLPITF
                     STELRTKTLATISEVRSLLNSGTTPL"
     gene            complement(558..1421)
                     /gene="cas7c"
                     /locus_tag="PWX02_000826"
     CDS             complement(558..1421)
                     /gene="cas7c"
                     /locus_tag="PWX02_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HDL1728858.1"
                     /translation="MSIQNRYEFVFFFDVTNGNPNGDPDAGNMPRLDPETSKGLVTDV
                     CLKRKIRNFIEMSYENEAGYEIYVKEKSVLNLQNKRAYEALGIESEAKKLPKEEAKAR
                     EITAWMCKNFFDIRTFGAVMTTEVNSGQVRGPVQLAFAQSIDPIIPLEVSITRMAVTN
                     EKDLEKERTMGRKYIVPYALYRVHGFISAKLAEKTGFSDEDVQKLWQALQLMFEHDRS
                     AARGEMAARKLIVFKHDSELGNQPAHKLFDSVKVERINGEKDTPAKDYDDYRISVQAE
                     GLTGVTVEELL"
     gene            complement(1431..3215)
                     /gene="cas8c"
                     /locus_tag="PWX02_000827"
     CDS             complement(1431..3215)
                     /gene="cas8c"
                     /locus_tag="PWX02_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HDL1728859.1"
                     /translation="MILSALTRYYYRLAAQEDRLTGEAKVPSYGFSEEKIGWVLELDS
                     KGNLVNVISNLTEDKKPKPKLMSVPRPEKRTSGIKPNFLWDKTAYVLGIESNPDKATA
                     KDQPINFAEKTFEAFKQYHLDLLADSNDEGLMAIRNFLQQWQPTHFAESICPREILDA
                     NVVFKLVGTSGYIHQREAAQTLWTSLLINQSDNAEKGLCLISGKLSPIQRTHPPIKGV
                     FGGQSSGGSIISFNKESFTSFGKEQGSNAPVSEQAAFAYTTALNYLLRTEKHRLTIGD
                     ASTVFWAEADNTTQAEAAEGFFAALMTPPDDEQENQKIFEELQKISKGRPLAEISPEL
                     SENTRFYVLGLAPNAARISVRFWLDTTFGQLAKNLSEHWQDLALEPCAWKTPPSIWRL
                     LLQTASLGKSENISPVLAGEMARAVISGHLYPMSLLSQLVTRIRADGDINGLRVALMK
                     AVLQRKFRKGFIKEGVPMSLNKESDNQAYLLGRLFAVLERIQTQALGELNAGIADRYY
                     GSASAVPFSVFPRLLSGAKHHLSRLRKDKGGMAVNLDKDLGEIIASLPENFPRHLSID
                     EQGRFAIGYYHQKQSYFAKKETTESIEN"
     gene            complement(3212..3889)
                     /gene="cas5c"
                     /locus_tag="PWX02_000828"
     CDS             complement(3212..3889)
                     /gene="cas5c"
                     /locus_tag="PWX02_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5"
                     /protein_id="HDL1728860.1"
                     /translation="MPNKIRLHIWGDYACFTRPEMKVERVSYDVITPSAARGILAAVH
                     WKPAIRWVIDKIYVLQPIQFESVRRNELGSKISASKISGAMKRKTVADLYTVIEDDRQ
                     QRAATVLKNVGYVIEAHAVLTKMAGEAETVTKHIEMFKRRAKKGQCFQQPCMGVREFA
                     ANFALIDDDEPLPECFLAENERDRDLGWMLHDIDFEHGNQPRFFRAEMKNGVIDVPPF
                     YAEEVKS"
     gene            complement(4127..5878)
                     /locus_tag="PWX02_000829"
     CDS             complement(4127..5878)
                     /locus_tag="PWX02_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDL1728861.1"
                     /translation="MANETLNFKDNARLGRVVTVDTSRVLISVEEHQQMTSIAVGNLI
                     AIQGRTASEYLIGIIERVTRSISEKLLLEEISNEGEIPLGESQDDFIKAVLIGTYKAV
                     DGSLSNTFKRGADCFPQIDRDCFHISGSNLQLFMNLLSANVKENERLTLGHFMADETA
                     VAIANGDKFFQRHAAILGSTGSGKSWSVALILERAAALKAANLIVFDMHGEYSTLCEG
                     KNAIAQQYRIAGPGDLDGSDERSLFIPYWLLNREEMLALILDRSDDNAPNQAARFTEH
                     VRALKEQTLNKEERNDVLETFTVDSPIPYSIELLLARLDKDNSEMVPKEGGSGTKQGP
                     WYSKLSRFMGRMKAKIDDRRCGFMFQPPVECNSYGWLENMLSKLFSASESEKGIKIID
                     FSEVPSDVLPIVTGVLARLLYNIQFWMDKDSRHPLTIICDEAHLYLPVRDSAEAAEKR
                     ALEAFERIAKEGRKYGVSLLVVSQRPSDVSRTILSQCNNFLVLRLTNDQDQSVVKRLL
                     PESMVGITDILPLLDLGEALLLGDSILLPTRIKLAKPHIAPESATRQFWSDWANQEPK
                     PNAVSNAVEALRRQVRS"
     gene            complement(5868..6941)
                     /locus_tag="PWX02_000830"
     CDS             complement(5868..6941)
                     /locus_tag="PWX02_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HDL1728862.1"
                     /translation="MEGKRKMREPLLDEDELKKWLQTIFTDNLVIIVGSGLSCAEGLP
                     GMGALADRLKERMPECLDDIDKVTWNTISDCLDSEGLEGALLKHQANETIEAAIIKIT
                     AEYVLSEEQKAINKCIAENQKLKFSYLLPHISASNPKIARVITTNYDRLIEFAAEYEN
                     WGIDSMMVGRYWGKHNPDLSRKLQIRDIRVKGKCPKLVYRDHIKIFKPHGSLDWFMAG
                     DVPMTSCIGKVEEPLIITPGVGKYKKGYGQPFDAHREKANEAIDSASSILCIGYGFND
                     DHLQTHLTGKIKSGVKTLLLTRGLSENARNVVKEAPNCKALIFDGDKAGTRVVSKQEE
                     TFIPNVHWWDVEFFVKEILENGK"
     gene            complement(7054..9342)
                     /locus_tag="PWX02_000831"
     CDS             complement(7054..9342)
                     /locus_tag="PWX02_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDL1728863.1"
                     /translation="MEEHTIQSLYAHSGKLSDHSDWQTLLSHANNVGLLAAEFAAPFA
                     SQEIARCTGLLHDLGKYSERYQKRLHGGERVNHSTAGAKIAVERWGPLGKMMAFCIAG
                     HHAGLANGSGEGKNRSTLTQRLQEQFGYGHKDLPVLDAIWQQELNLPEKLPLPPLKPS
                     KNEPFFSYAFFIRMLYSCLVDADFLDTEAFYANLEKRPLERGNSPNLTALHQQFNQFI
                     AQFRERITIQTPNTQEAQRKAEVNRLRSEILDYAVSQVKQETGLFSLTVPTGGGKTFT
                     SMAFALEHAKQYGLCRIIYVIPFTSIIEQNAAEFRKAFGELGEEAVLEHHSTFDHDSL
                     PDKSSRDKLKLASENWDAPVVVTTAVQFFESLFADRSSRCRKLHNIAGSVIILDEAQM
                     LPLNLLRPIMAAIDELARNYQCSVVLCTATQPAIHQANGFYKGFENVREIAPNPTALF
                     EKLKRTTVKHIGIQTDVELQSKLADNPQMLIIVNNRRHARALYDSAKQLEGAYHLTTL
                     MCAKHRSQTLTEIRLRLKQNLPCRVIATSLIEAGVDVDFPLVMRAEAGLDSVAQAAGR
                     CNREGKRLSVESNVWVFQPEEQWKAPPELGLLSGCMRSTVQVHADDLLNPKAINHYFQ
                     AVYEAKGKELDKPNILKACHDAGQSLNFPFQKIAEDFRMITSHLIPVIIPFDQEAENL
                     IDRLRYADQVGGILRKLQPYTVQVPEKALAELYNAGRVETINETNFGKQFYCLIGMDL
                     YDEVAGLSWENVDFLESEKLVL"
     gene            9605..11368
                     /locus_tag="PWX02_000832"
     CDS             9605..11368
                     /locus_tag="PWX02_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HDL1728864.1"
                     /translation="MSEQTFITGKDAALEDSIATFQHKLKTLGFNIEEASWLNPVPNV
                     WSVHIRDADCPQCFSNGKGASKKAALASALGEYFERLSTNYFWADFYLGQEIANSEFV
                     HYPTEKWFPIDDEAIPPMEMMDEFLWEYFDPNGEITPELLVDLQSGNYDRGIVTLPYV
                     RQSDNETVYIPQSIIGNLFVSNGMSAGNTKNEARVQGLSEVFERFVKNRIITEAISLP
                     EIPQSVIDGYPTIKASIEKLEQEDFPILCYDASLGGEFPVICVILLNQNNGTCFASFG
                     AHPNFQVAFERTVTELLQGRSLKDLDVFAPPSFNNEDVADHANLETHFIDSSGLISWD
                     LFKRDADYPFVHWDFSGTTEQEFNNMMAIFNKHNQPVYIMDYEHLGVYACRILAPGMS
                     NIYPSDDLVYANNNMGMDWREILLDLPHFHHDAETYQELLDELDEQSIDDLTRVREFI
                     GVVAEKGSAMQTLRIGELKSMLHLALGNLEQASDWANWTANMNASVFSAERNNYYRCL
                     IQSIELFLDEERDSEQYRMVFEKMYGKQAVEAAWGAIQGGNPFHGLTASDESLENMTA
                     HQKLLNAYRKVQKAKVNPIAR"
     gene            11465..12427
                     /gene="cmoB"
                     /locus_tag="PWX02_000833"
     CDS             11465..12427
                     /gene="cmoB"
                     /locus_tag="PWX02_000833"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005707797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HDL1728865.1"
                     /translation="MIDFRPFYQQIATTDLSAWLETLPLQLKQWEKTTHADYAKWAKV
                     ADFLPNSTACINLKDKVESISSKPLSEGEVKQITHHLKQLMPWRKGPYHLHGIHIDTE
                     WRSDFKWDRVLPHLAPLKDRTILDVGCGSGYHMWRMVGEGAKMVVGIDPTELFLCQFE
                     AVRKLLGNDRRANLIPLGIEQMQPLAAFDTVFSMGVLYHRKSPLDHLSQLKAQLVRGG
                     ELVLETLVIDGDINDVLMPADRYAKMKNIYFIPSVAALINWLEKVGFKNVRCVDEAIT
                     TLEEQRKTDWLENESLVDFLDPNDHSKTIEGYPAPKRAVILANA"
     gene            complement(12480..14912)
                     /gene="gyrB"
                     /locus_tag="PWX02_000834"
     CDS             complement(12480..14912)
                     /gene="gyrB"
                     /locus_tag="PWX02_000834"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDL1728866.1"
                     /translation="MSEQTQATSYDSSSIKVLRGLDAVRKRPGMYIGDTDDGTGLHHM
                     VFEVVDNAIDEALAGHCKDIIVTIHSDNSVSVQDDGRGIPVGIHPEEGVSAAEVIMTV
                     LHAGGKFDDNSYKVSGGLHGVGVSVVNALSSKLQLTIRREGHVHEQFYSLGEPDAPLA
                     IIGNTEKTGTSVRFWPSLDIFKNKTEFEYKILSKRLRELSFLNSGVSIKLIDERDGKE
                     EHFKYEGGIKAYVEYLNEGKTHIHNTPFYLTTEKDGIGVEISLLWNDSYNENVYCFTN
                     NIPQRDGGTHLAGFRGALTRALKNYMDNSGVLKKADANIDASGDDAREGLVAVISVKV
                     PDPKFSSQTKDKLVSSEVRGAVESSMNEALSDYLAENPDDAKNIVTKIIDAARAREAA
                     RKAREMTRRKGALDLGGLPGKLADCQEKDPALSELYLVEGDSAGGSAKQGRDRKNQAI
                     LPLKGKILNVEKARFDKMLSSQEVATLITALGTGIGRDDYNLEKLRYHKIIIMTDADV
                     DGAHIRTLLLTFFYRQMPELIENGYIYIAQPPLYKVKKGKQERYIQDNDEMSQYEIDI
                     ALEGAGLYVSEDAPALSGVALENLIAQYNGVQKLIERLSRRYPTLLLNELIYSKPLSV
                     EFAKNQANMTAWSEQFVATLMAKEVGGSFYRINTLFNEERQIYEPEIIVTTHGMDSTY
                     RLDYNFINGNEYARIVALGNELNDLLSDSAYVVRGERRQEISSFAEALDWLVKESRKG
                     LTIQRYKGLGEMNPEQLWETTMDPVARKMLQVNITDAIAADKLFSTLMGDEVEPRRDF
                     IETNALYAQLDI"
     gene            complement(15044..17680)
                     /locus_tag="PWX02_000835"
     CDS             complement(15044..17680)
                     /locus_tag="PWX02_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL1728867.1"
                     /translation="MELQFETLEYQLQAVNATVNLFVGQPNAATEFSLKAQNDMRFVP
                     NLGLQISDEQLQQNLANLQNRQKIDRTLLVEQGKNFTVEMETGTGKTYVYLRTIFELN
                     RQYGWQKFVIVVPSVAIREGVLHTLETTKSHFNTVFDNPSVNQKFEYKSNQISRLKSF
                     ASANHIEILVMNIDSFTKESNVINTVNESGDAPIFYIQQANPIVIIDEPQNMETEIRR
                     NAIESLSPLFTLRYSATHKNAYNPIFSLNPVQAYEMGLVKQIEVDSVLAENEVNGAYL
                     SLKEIKTGAKSWSAKIEISFNDKKAMKKKVISVKPNQDLYELSNQNEMYRSGFILSGM
                     DTDSQMVSFSNGTQIFQGAEHSQLKEDVQKMQIRRTIGEHLQKEKKLRPLGIKVLSLF
                     FIDKVDNYRPSAQSPTGGKFAKWFEEIYLELAKEEPQGVHNGYFSQDKKGVAKDTNGT
                     TQADNETYQLIMRDKEKLLSLENPLRFIFSHSALKEGWDNPNVFQICTLNDTASEMKK
                     RQEIGRGLRLPVNQQGLRIYDRHLNTLTIIANESYEQFAATLQREIEEDCGVQFAKSN
                     VKKREDKKAVRYRKDFSLHPEFAAIWERIKHQTTYRVDFSNAELIESAVEKIRKMPDI
                     DATKIKHSKVKLEMNETGISTNYRSSNETAVNKQWAIPDLLSEIQKKTGLTRATLCKI
                     LQKSDRLQEVFRNPQRFIDLIAEQINLALQQIMTDGVEYHKIAGKTYEMTLFGDFDFY
                     LNQYTFEVQDKTKTIYEDYIALDSQTENIFARHCESSEQVEFYFKLPKRFKIPTPLGS
                     YNPDWAVVFKGENKIYLVAETKNTGKGIQAGVDEEKLDESERMKIDYARKHFKGLQRD
                     YKDLDYRVVQKVEELSVEYCKI"
     gene            complement(17690..>18530)
                     /locus_tag="PWX02_000836"
     CDS             complement(17690..>18530)
                     /locus_tag="PWX02_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL1728868.1"
                     /translation="LNNKNYKAVSAGNLTNYTLDVKTTSAGTLLKELFETKLPVFSAP
                     KNPNFLRHLITLFEDKNFISLDFFAGSSSFPHAILESNRIDKGNRKFIAVQYPEEIDI
                     KSKNGKVAKQFCQRKNLPLYITEISKERIRRAGKQILQNNPENRPLDVGFKVFKLTES
                     HFKQWQSPTSENLADQLEMFIDPVSEKADTQAVLYELLLRLGLKLTAKVRLENKVFWV
                     EENGLIFALLLNAADEEIIQTVIAQQPKKVIALDRLFNGNDARKKNTELQMQDAGITF
                     FVI"
BASE COUNT         5149 a         4079 c         3548 g         5754 t
ORIGIN      
        1 gagtggtgtt gttccgctgt ttaaaagtga ccgcacttcg gaaatggtgg cgagggtttt
       61 ggtgcgtagt tcggtggaga aagtgatcgg caagcggtgg cgggtttgca tataccacaa
      121 tgctccctcg ttaatagttt gccccgtcat ttcttccaaa cataacgctt gggcgcaaag
      181 ttggatttcg tccatcggtt caggttttgg cttgccacgt ttatattcca ccggttttag
      241 ttcgcctgtt ttcagatctc gctcgaccaa atccaaaata ccactgatgc ctaatttttc
      301 cgctgcaacg tgaaccgttc gctcaaagcg aatgcccttt cgggtttctg gctcaccact
      361 atctacccgc tcgtgcaaca cttgaccttg tgcggtgaga tagttttctg cccacacctg
      421 ttcgttatga attaacgcac attgacgtgg gcagaaggcg tagtgttgca aagcggaaag
      481 cgaaatcgac cgcttgtcat agccaaattt tggctgaaca agcggtaaaa tttccgtaga
      541 attttgcaaa tcgtccatta cagcaattct tcaacggtta cacccgttaa gccttcagct
      601 tgtacgctga tgcggtagtc gtcatagtct ttcgctggcg tgtctttttc accgttgatt
      661 cgctcaactt ttaccgagtc aaacagtttg tgtgcaggtt ggttgcctaa ttcgctgtcg
      721 tgtttaaata cgatcagttt acgagctgcc atttcgcctc gtgcggcaga acggtcgtgt
      781 tcaaacataa gttgcaaggc ttgccaaagt ttttgtacat cttcatcaga aaagcccgtt
      841 ttttcagcga gtttagctga aataaagccg tgaacacgat aaagtgcgta aggcacgata
      901 tatttacgcc ccatagtccg ttctttttca agatcttttt cgttggtcac tgccattcgt
      961 gtgatcgata cttctagcgg aataatcgga tcgatagatt gagcgaacgc caattgcact
     1021 gggcctcgca cctgtccgct gtttacttcg gtagtcatta ccgccccaaa ggtgcgaata
     1081 tcgaaaaagt ttttgcacat ccaagcggta atttctcggg ctttagcttc ctcttttggt
     1141 agttttttcg cttccgattc aatccctaat gcttcataag cacgtttgtt ttgtaaattc
     1201 agaacgcttt tttctttaac atagatttcg taacccgcct cgttttcata gctcatttca
     1261 ataaaattgc ggattttgcg ttttaggcaa acatcggtga ctaaaccttt actggtttca
     1321 ggatcaaggc gtggcatatt tcctgcatct ggatcaccat ttgggttacc gttggttaca
     1381 tcaaagaaaa aaacaaattc atagcggttt tgaatagaca tagtgtttcc ttagttttca
     1441 atagactcag tggtttcttt tttagcgaaa tagctttgtt tttggtggta atagccaatc
     1501 gcaaagcggc cttgctcatc gatacttaaa tggcgaggga aattttccgg caagctggca
     1561 ataatctcac ctaaatcctt atccagatta actgccattc cgcccttatc ttttctcaag
     1621 cgagataaat gatgttttgc tccgcttaat aagcgaggga ataccgaaaa tggcacggca
     1681 gaggctgaac cataataacg gtcggcaatg ccggcattta attcacctaa cgcttgggtt
     1741 tggattcttt ctaataccgc aaataacctg ccgagcaagt aagcttggtt atcgctttct
     1801 ttatttaagc tcataggaac tccttcttta ataaaacctt ttctaaattt tcgttgtaag
     1861 accgctttca tcaatgctac tcgcaagccg ttaatatctc cgtctgctcg gattcgagtc
     1921 accagctgag aaagcaagct catcgggtat aaatgaccgc taataaccgc ccgagccatt
     1981 tcgcccgcca agacaggaga aatattctca cttttaccta atgaagcagt ttgtaataaa
     2041 agtcgccaaa tggagggagg cgttttccat gcacaaggct caagggcaag atcttgccaa
     2101 tgctcgctaa gattttttgc cagttgccca aaggtggtat ccagccaaaa acgcaccgaa
     2161 atgcgagctg cattcggagc aagccccaac acataaaaac gggtgttttc agataattct
     2221 ggggaaattt ctgcaagcgg tcgtcctttg ctgatttttt gcaattcttc aaagattttt
     2281 tggttttctt gctcatcatc tggaggcgtc attaaagcag caaaaaagcc ttcagcagct
     2341 tcagcttggg tggtattgtc tgcttctgcc caaaagacag tgctggcatc accaatggtt
     2401 aaacgatgtt tctcggtgcg taacaaataa tttaaagcag tagtgtaggc aaaggcggct
     2461 tgttcagaaa caggggcatt actaccttgt tccttgccaa atgaagtgaa ggattcttta
     2521 ttaaaagaga tgattgaacc accggaactt tgcccaccaa ataccccttt aataggagga
     2581 tgagttcgtt gaattggcga taatttgcct gaaattaagc ataaaccttt ttcagcatta
     2641 tcactttgat ttatcaacaa acttgtccaa agggtttgtg cagcttctcg ctgatgaata
     2701 taaccgcttg tgccgactaa tttaaacacg acattcgcat caagaatttc tcttgggcag
     2761 attgattcgg caaaatgtgt gggttgccat tgttgcagga aatttcttat cgccattaaa
     2821 ccttcatcat tggaatctgc aagcaaatcc aaatggtact gtttaaatgc ctcaaaggtt
     2881 ttctcggcga aattgatggg ttgatctttg gcggtggctt tatcaggatt actttcaatg
     2941 cctagcacat aagcggtttt atcccacaga aaattgggct taatgccaga cgtgcgtttt
     3001 tcagggcgag gtacgctcat caatttcggt tttggtttct tatcttctgt taaatttgag
     3061 atcacattga ccaaatttcc ctttgaatct aattctagta cccaaccgat tttttcttca
     3121 ctgaaaccat aagaaggcac tttagcttcg cctgttaagc gatcttcttg tgctgcaagg
     3181 cggtaataat agcgagttaa ggcggagaga atcatgattt gacctcctct gcataaaatg
     3241 gtggcacatc aatcacgcca tttttcattt ccgcacggaa gaaacgaggt tgattaccgt
     3301 gctcaaaatc aatgtcgtgc aacatccagc ctaaatcccg atctcgctca ttttctgcca
     3361 aaaaacactc aggtaacggt tcatcgtcat caatcaaggc aaaatttgcg gcaaattcac
     3421 gcacgcccat acaaggctgc tggaaacatt gccccttttt cgcacggcgt ttaaacattt
     3481 caatatgctt ggtgacagtt tccgcctcgc ctgccatttt ggtcagcact gcgtgagctt
     3541 cgatcacata accgacattt ttcagcacag tagcagcacg ttgttggcgg tcgtcttcaa
     3601 tcacagtata taaatccgcc actgttttac gcttcattgc accactgatt ttactggcag
     3661 aaattttact gcctaactca ttacggcgaa cagactcaaa ttgaatcggt tgtaatacat
     3721 aaattttatc aatcacccag cgaattgccg gcttccaatg caccgcagcc aaaatccccc
     3781 gtgccgcaga cggcgtaatc acatcataag acacccgttc taccttcatt tcaggacggg
     3841 taaaacaagc ataatcaccc caaatatgga gcctgatttt gtttggcata atagaatgct
     3901 ccttttaatt atgtaacaca cacctccccg aagggaggtg aaatttcttt agcttataac
     3961 tgctttggtt tcaacccacg cactcggtaa aatgcgaaaa attttatggc ttattatatt
     4021 aagcggtcgg atttacttag aaagttgcaa agttatttga gtatttaacc gcttgtattt
     4081 caaaatagtc agaaaaataa cagcactttc taggtacaat atttttctaa gaacgaactt
     4141 gtctccgcaa tgcctctact gcgtttgaca cagcatttgg tttgggctcc tggtttgccc
     4201 aatcagacca gaattggcgt gtagcacttt ctggggctat atgcggttta gcaagtttga
     4261 ttcgtgtagg taatagaatc gaatcgccta gcaataacgc ttcgcctaaa tcaagtaacg
     4321 gcaatatatc agtaattcca accatagatt ccggcaataa acgctttaca acactttgat
     4381 cttgatcatt tgttaatcgt aaaaccaaga aattattaca ttgactcaaa attgttcggc
     4441 ttacatctga aggacgttgg ctaacgacca acaaagacac gccatatttt ctaccttctt
     4501 tcgcaatccg ctcaaaggct tccaatgcgc gtttttccgc tgcttccgct gaatctcgaa
     4561 cagggagata taaatgtgct tcatcgcaaa taatcgttag tggatggcgg gaatctttat
     4621 ccatccaaaa ttgaatgttg tataaaaggc gggcaagaac tcccgtaaca atcggtaaaa
     4681 catccgacgg gacttccgaa aaatcaataa tttttatccc tttttcgctc tcagaagcag
     4741 aaaacagttt tgacaacata ttttccagcc acccatagct gttgcactct actggcggtt
     4801 ggaacataaa accgcaacgt ctgtcatcta ttttggcttt catccttccc ataaacctag
     4861 acaacttact ataccaagga ccttgctttg ttcctgaacc tccctcttta ggtaccatct
     4921 cagaattatc tttatctaaa cgagctaaca ataattcaat agaatacgga attggcgaat
     4981 caacagtaaa cgtttccaaa acatcatttc tttcttcctt attcaatgtt tgctctttta
     5041 atgcgcgaac atgctcagta aaacgcgccg cctgattcgg agcattatca tcactgcgat
     5101 ctaaaattaa agccagcatt tcttcccgat tgagcagcca atagggaata aataaactgc
     5161 gttcatctga accatccaaa tctcccggtc cagcaatacg gtattgctga gcgatagcgt
     5221 ttttaccttc gcatagagtg ctatattcac cgtgcatatc gaatacaata agattagcag
     5281 cttttaaagc tgctgcacgt tccaatatta atgcaactga ccagctttta ccggaaccag
     5341 tactacccaa aatagcagcg tgccgttgaa agaatttatc tccgtttgct atagctacag
     5401 ccgtttcatc agccataaaa tgacctaaag tcagccgttc attttctttt acgttagcgg
     5461 acaaaagatt cataaataat tgcagattgc tgccactaat atgaaagcaa tctctatcaa
     5521 tttgcggaaa acaatccgct ccccgcttaa aagtgttgct caaactacca tcaacagctt
     5581 tataagtacc gattaatact gccttgataa agtcatcctg actttcaccc agaggaattt
     5641 caccctcatt agatatttct tctagcagca atttttcact gatagaacgc gttacccgtt
     5701 cgataatgcc gataaggtac tcactagccg ttctgccttg aatcgcaatc agattaccca
     5761 cagcaatact agtcatttgt tgatgctctt caacagaaat caatacacgg ctggtatcta
     5821 ctgttacaac cctgcccaaa cgggcattgt ctttaaaatt caatgtttca tttgccattc
     5881 tctaaaatct cttttacaaa aaactcaacg tcccaccaat gaacattagg aataaaagtt
     5941 tcttcttgct tgctaaccac ccttgttcca gccttatctc catcaaaaat cagagcctta
     6001 caattcggag cttccttcac cacgtttctg gcattttctg aaagtcctct tgtcagaagt
     6061 agtgttttca caccactttt aattttgcct gtcaaatgtg tttgaaggtg gtcatcatta
     6121 aaaccatagc cgatacataa aattgaagaa gcagaatcaa tagcttcatt tgctttttct
     6181 cgatgagcat caaatggttg tccatagcct tttttatact tcccaacacc aggcgtaata
     6241 attaaaggct cttccacttt acctatacaa cttgtcattg gaacgtctcc agccataaac
     6301 caatctaaac taccatgcgg tttaaagatt ttgatatgat ctcgataaac caattttgga
     6361 catttacctt ttactctaat gtctcttatc tgcaatttcc tactcaaatc aggattatgt
     6421 tttccccaat aacgaccgac catcattgaa tctattcccc aattttcata ctctgccgca
     6481 aactcaatca atctatcata attagtagta attacacggg caattttagg atttgaagct
     6541 gaaatatggg gaagcaaata agaaaatttg agcttctgat tctcagcaat acatttgttt
     6601 atcgcttttt gctcttcgga gagtacatac tcggctgtta ttttaataat agcggcttct
     6661 atcgtctcat ttgcttgatg cttcaacaat gcaccttcca agccttcaga atccagacaa
     6721 tcagaaatgg tattccatgt taccttatct atatcatcaa gacattcagg cattcgttct
     6781 tttaatctat cagctaatgc tcccattccc ggcaaacctt cggcacagga caaacccgaa
     6841 ccgacaataa tgaccaaatt gtcagtaaaa atagtttgca accatttttt taattcatct
     6901 tcatctaata atggttctct cattttacgt ttcccttcca aatatctatt cttacaaact
     6961 agctgtttac tacgagacgt cataggctat ttggtatatt aaagtatatc tatgcaaaat
     7021 taaacttata aagaagttaa aataaacatt catctataaa accaatttct ccgactcaag
     7081 aaaatccaca ttctcccaac tcaaccccgc cacttcatca tacaaatcca ttccaattaa
     7141 gcagtaaaac tgtttgccga agttggtttc attaatggtt tcaactctgc cagcgttata
     7201 gagttcggct aatgcttttt ccggtacttg cacagtgtaa ggctggagtt ttcttaaaat
     7261 accgcctact tgatccgcat agcgtaaacg gtcaataagg ttttcggctt cttggtcgaa
     7321 agggataatc accggaatta aatggcttgt gatcattcgg aaatcttcag ctattttttg
     7381 gaatgggaaa ttaaggcttt gcccagcgtc gtgacacgcc tttaaaatat ttggcttatc
     7441 cagctctttt cctttagcct cataaaccgc ttggaaataa tgattgattg ctttcggatt
     7501 aagtagatca tctgcgtgaa cctgaaccgt tgaacgcata cagccagaaa gtaagcctag
     7561 ctctggtggt gctttccact gttcttcagg ttggaaaacc caaacattgc tttctacaga
     7621 taaacgtttc ccctctcggt tgcaacgccc tgctgcttga gccactgaat ctagtcctgc
     7681 ttcagctcgc ataaccagtg ggaaatcgac atcaacaccg gcttcaatta aagaagtggc
     7741 aatcactcgg caaggtaggt tttgttttag gcgaagacga atttcagtca gcgtttgtga
     7801 gcggtgttta gcacacatta gcgtggtgag gtgataagct ccctctaact gtttcgctga
     7861 atcatataac gctcgggcgt gacggcggtt attcacgatt atcagcattt gtgggttatc
     7921 tgcaagtttg ctttgcaact ccacatctgt ttgaataccg atatgcttta ccgtagtacg
     7981 tttcaatttt tcaaaaagtg cggttggatt aggggcaatt tctcgcacat tttcaaatcc
     8041 tttataaaaa ccgttcgctt gatgaatagc gggttgtgtc gctgtgcaaa gcactaccga
     8101 acattgataa ttgcgagcta attcatcaat cgcagccata attgggcgta ataaattcaa
     8161 cggtagcatt tgggcttcgt ccaaaataat cacgctgccg gcgatgttgt gcaatttgcg
     8221 gcaacgtgaa gaacggtcgg caaacaaact ttcaaagaat tgcactgcag ttgtaaccac
     8281 aacaggagca tcccaatttt ccgatgccaa tttcagctta tctcggcttg atttatcagg
     8341 taggctatcg tgatcaaaag tgctgtgatg ctccaataca gcctcttctc ccagctcgcc
     8401 aaaagcttta cgaaattccg cagcattttg ttcaataatg ctggtaaacg gaataacata
     8461 aataatgcgg cataaaccat actgtttagc atgctcaagg gcaaacgcca ttgaagtaaa
     8521 ggttttacca ccgcccgttg gcacagtcag agaaaacaag cctgtttcct gtttcacttg
     8581 agatacggca taatccaaaa tctcactccg caagcggttt acttccgctt tacgttgtgc
     8641 ttcctgcgta ttaggcgttt gaatggtaat tcgctcccta aattgtgcaa taaattgatt
     8701 aaattgctga tggagtgcgg ttaaattggg ggaatttcct cgttcaagcg gtcttttttc
     8761 gagattggca taaaaagctt ctgtatcaag aaagtcggca tcgacaaggc aagaatagag
     8821 cattcgaata aaaaaggcat aagaaaagaa aggttcgttt ttagaaggct ttaacggcgg
     8881 taagggcaat ttttcaggca aattaagttc ttgctgccaa atagcgtcaa gtaccggtag
     8941 atctttatga ccatagccaa attgctcttg caaacgttgt gtgagtgtag aacgattttt
     9001 cccttctcca gaaccatttg ctaacccagc gtggtgtcct gcaatgcaaa acgccatcat
     9061 tttgcccaga ggcccccaac gttccactgc aatttttgca ccggcagtag agtgatttac
     9121 ccgttctccg ccatgtaaac gcttttggta acgttcacta tatttgccca aatcgtgcaa
     9181 tagccccgtg cagcgagcaa tttcttgtga agcaaacggc gcagcaaatt cggcggcaag
     9241 caagccaaca ttattggcgt ggctgagtaa ggtttgccaa tctgaatgat ctgaaagctt
     9301 ccccgaatgg gcatacaaag actgaatagt atgttcttcc attttatacc ttcaatattt
     9361 ccttcctaac acaagcaata ctacctctaa ccttttactc tgtatatatc ctagataata
     9421 ttttcttaat ctaaatcaaa cacaaatttc acttgcaaaa ttcaagtaaa atttgaccgc
     9481 ctgtaaccat tttttctaag ttgtgaaccc tgttctttca gcagaaattt cacaaaaaat
     9541 ttacttatct atggggattt ttgctagaat gttgccattt ttacgacaag ttaaaggtat
     9601 tatcatgtca gaacaaactt ttattaccgg taaagatgct gcacttgaag acagcattgc
     9661 gacctttcag cataaattaa aaaccttagg ttttaatatt gaagaagcct cttggttaaa
     9721 tcctgttccg aatgtgtggt cggtgcatat tcgtgatgca gactgcccgc aatgtttttc
     9781 taacggtaag ggagcaagca aaaaagccgc tcttgcctct gcattaggtg agtattttga
     9841 gcgtttatct accaactatt tctgggcgga tttttattta ggtcaagaga ttgctaatag
     9901 tgaatttgtg cattatccaa ccgaaaaatg gtttccgatt gatgatgaag ccattccacc
     9961 aatggagatg atggacgagt ttttatggga atattttgat ccgaatggtg aaatcacgcc
    10021 ggaattattg gttgatttac aatcaggaaa ttatgatcgt ggaattgtta ccctacctta
    10081 cgttcgccaa tctgacaatg aaacggttta cattccgcaa agcattatcg gcaacctgtt
    10141 tgtttcaaac ggtatgtcgg caggcaacac aaaaaatgaa gctcgagtgc agggcttatc
    10201 cgaagtgttc gagcgttttg tcaaaaaccg tattattact gaggcaatca gcttgccgga
    10261 aattccgcaa agtgtgattg acggttatcc gacaatcaaa gcctctatcg agaaactaga
    10321 gcaagaagac ttcccgattt tatgctatga cgcttcatta ggcggagaat ttccagttat
    10381 ttgtgtgatc ttgttaaacc aaaataacgg tacttgtttt gcctcttttg gtgcacaccc
    10441 gaatttccaa gtggcgtttg aacgtaccgt aaccgagtta ttacaaggcc gtagcttaaa
    10501 ggatttagat gtgtttgccc caccttcatt taataatgaa gatgtagcag atcatgcgaa
    10561 ccttgagaca cactttattg attcaagcgg tctaatttcg tgggatcttt tcaaacgtga
    10621 tgcggattat ccgttcgtgc attgggactt ctccggcaca acggagcaag aatttaataa
    10681 tatgatggcg attttcaaca agcataatca gcctgtgtat attatggatt atgagcattt
    10741 aggtgtgtac gcttgccgta ttcttgcacc gggtatgtcg aatatctatc caagcgatga
    10801 tttggtctat gccaataaca atatgggtat ggattggcgt gaaatcttgt tagatttgcc
    10861 acatttccat cacgatgctg aaacttacca agaactgctt gatgaattag atgagcaaag
    10921 cattgatgat ttaacccgtg tgcgtgaatt tattggcgtg gtggcagaga aaggctctgc
    10981 aatgcaaaca ttgcgtatcg gtgagcttaa atcgatgctg catttagctt taggtaactt
    11041 agagcaagcc tcagactggg caaactggac agcgaatatg aatgcaagcg tattctcagc
    11101 agaacgtaac aattattacc gctgcttaat ccaatcgatt gagttgttct tagatgaaga
    11161 gcgtgattcg gaacaatatc gtatggtgtt tgagaaaatg tacggtaaac aagcggttga
    11221 agctgcttgg ggtgcaatcc aaggcggtaa cccgttccac ggtttaactg catcggacga
    11281 atcgcttgaa aatatgactg cacatcagaa attactgaac gcttatcgca aagtgcaaaa
    11341 agcgaaagta aatccgattg cgagataatt ttatattttt cgtaggggta ggttctgtgc
    11401 ctgtcctttc tatttactac ctttcgggag ggcataggtc tctcccctag acataaattt
    11461 caaaatgatt gactttcgtc ctttttatca acaaattgcc acaaccgacc tttctgcttg
    11521 gttagaaacc ttacccttac aactcaaaca atgggaaaag accacccacg ctgactatgc
    11581 caagtgggca aaagttgcag attttttacc aaattcgacc gcttgtatca atctaaaaga
    11641 caaggttgag tctatttcaa gcaagccttt atctgagggg gaagtcaagc aaattaccca
    11701 ccatttaaag caactaatgc cgtggcgtaa aggcccttat catctacacg ggattcatat
    11761 tgataccgaa tggcgttccg attttaaatg ggatcgtgtg cttccgcatc ttgcaccgtt
    11821 aaaagaccgc accattttag atgtcggctg cggtagcggt tatcatatgt ggcgaatggt
    11881 aggcgaaggg gcaaaaatgg tagttggtat tgacccgacc gagctgttcc tgtgccaatt
    11941 tgaggcagtg cggaaactct tagggaatga tagacgggct aatctgattc cacttggtat
    12001 tgagcaaatg caaccgcttg ctgcttttga taccgtgttt tcaatggggg tgctttatca
    12061 tcgcaaatca ccgcttgatc atctttctca actcaaagcc caattagtca gaggcggtga
    12121 attggtgtta gaaacgctgg tgattgatgg cgatattaat gatgttttaa tgccggctga
    12181 tcgctatgcg aaaatgaaaa atatctattt tattccttct gttgctgcac tcattaattg
    12241 gctggaaaaa gtcggcttta aaaatgtgcg ttgtgttgat gaggcaatca ctacactaga
    12301 agagcagcga aaaaccgatt ggttggaaaa tgaaagttta gtggattttt tagatccaaa
    12361 cgatcatagc aaaaccattg aaggctaccc tgctccgaag cgggcggtga ttttagcgaa
    12421 tgcttaaaat gctagtttaa gagaataaaa aatccgcatg tcaaatgcgg attttttaac
    12481 taaatatcta actgagcata caaagcgtta gtttcaatga aatctcggcg tggttcaact
    12541 tcatcgccca tcagcgtact gaagagttta tctgccgcaa tcgcatcggt aatattgacc
    12601 tgtagcattt tacgagccac cggatccatc gtggtttccc ataattgttc cggattcatc
    12661 tcacctaacc ctttataacg ttgaatagtt aagcctttac gagattcttt caccaaccaa
    12721 tctaaggctt cagcaaaact gctgatttct tggcgacgtt caccacgcac cacataggct
    12781 gaatcagata gtaaatcgtt cagctcgttg cctaatgcca caatgcgagc gtattcatta
    12841 ccgttgataa agttgtaatc taaacgatag gtactgtcca taccgtgtgt agtcacaata
    12901 atttccggct cataaatttg gcgttcttca ttgaacaagg tattaatacg gtagaaactg
    12961 ccgcccacct cttttgccat caatgtagcg acaaattgct cgctccaagc ggtcatattt
    13021 gcttgatttt ttgcaaattc caccgaaagc ggttttgaat aaattaattc gttaagtaat
    13081 aatgttgggt aacgacggct taaacgttca attaattttt gtacgccgtt atattgggca
    13141 attaagttct ctaacgctac gccacttaac gctggagcat cttcactcac atataagcct
    13201 gcaccttcta atgcaatatc aatttcatat tgcgacattt catcgttatc ttgaatataa
    13261 cgctcttgct tacctttttt cactttataa agcggtggct gtgcaatata aatatagccg
    13321 ttttcaatca gctccggcat ttgacggtag aagaaagtta ataaaagcgt acgaatatgt
    13381 gcaccatcca cgtccgcatc ggtcataata atgattttgt ggtaacgtaa tttttccaaa
    13441 ttataatcat cacgcccgat gcctgtgcct aaagcggtaa tcaaggttgc tacttcttgt
    13501 gaagaaagca ttttatcaaa gcgggctttt tccacgttca gaattttccc ttttaacggc
    13561 aaaatcgctt ggtttttacg gtcacgccct tgttttgccg agccaccggc agagtcaccc
    13621 tccaccaagt aaagctccga taacgccgga tctttttcct gacaatcagc taatttaccc
    13681 ggtaagccgc ctaaatctaa cgccccttta cggcgggtca tttcacgggc tttacgagct
    13741 gcttctcttg cacgggctgc atcaataatt ttggtaacga tattttttgc atcgtccgga
    13801 ttttcggcta aatagtcgga taaagcctca ttcattgagc tttccactgc tccacgcact
    13861 tctgacgaca ccaatttatc tttggtttgt gaggagaatt tcggatccgg cactttcacc
    13921 gaaatcaccg ctactaaacc ttcacgggca tcatcacctg aggcatcaat gttcgcatcg
    13981 gcttttttca ataccccgct gttatccata tagtttttca acgcacgagt taatgcccca
    14041 cggaaaccgg ctaagtgcgt accgccatca cgctgtggaa tattattggt aaagcagtac
    14101 acattttcgt tgtaactgtc attccacagt agggaaattt ccacgccaat gccgtctttc
    14161 tcagtggtca aatagaatgg cgtattgtga atatgagttt tgccttcgtt taaatactca
    14221 acataggctt taataccgcc ctcatattta aaatgctctt ctttgccgtc tcgttcatca
    14281 attaatttaa tcgaaacgcc tgaattcaaa aatgatagtt cacgcaagcg tttagacaga
    14341 attttatatt caaattcggt tttatttttg aaaatatcca aacttggcca gaaacgaacc
    14401 gacgtccccg ttttttcggt attgccgata atcgctaaag gtgcatcagg ctcacctaag
    14461 ctatagaatt gctcgtgaac gtgtccttca cggcgaatag tgagttgtaa tttagaggaa
    14521 agtgcattca ccaccgatac gcccacaccg tgcaaaccgc cggaaacctt ataggagtta
    14581 tcgtcaaatt taccgcctgc gtgaagaaca gtcataatga cttctgctgc cgaaacgcct
    14641 tcttccgggt gaatccccac cggaattcca cgaccgtcat cttgcacaga aaccgaatta
    14701 tcggaatgaa ttgtgacaat aatgtcttta caatgcccgg ctaatgcttc gtcaatcgca
    14761 ttatcaacca cctcaaacac catatggtgt aagcccgtac cgtcatcggt atcgccgata
    14821 tacattcccg ggcgtttacg caccgcatct aagccgcgta acaccttaat actggaagaa
    14881 tcataacttg ttgcttgagt ttgttcagac attcttattc ctactaattt gaaatcgaaa
    14941 aaattgtgcc gattatagca gaaaaatgcg taaaagtatc gcaaaacaag cggttaaatt
    15001 ttagttgaaa ttgcagtcta tctcaattct acaagaccac agattaaatt ttacaatact
    15061 ccacagacag ctcctccact ttctgtacaa ccctataatc caaatcctta taatctctct
    15121 gtaagccttt gaaatgctta cgggcatagt cgattttcat tcgttcactt tcatctaatt
    15181 tttcttcatc tactcctgct tgaatacctt tgcctgtatt tttcgtttcg gcaaccaagt
    15241 aaattttatt ttctccctta aacacaactg cccaatcggg attatagctg cctaatggcg
    15301 ttggaatttt aaaacgtttg ggtaatttaa aataaaactc cacttgctca ctagattcac
    15361 aatgtcgggc gaagatattt tctgtttggg aatccagtgc aatatagtct tcataaatcg
    15421 tttttgtttt atcttgaact tcaaaagtat attgattgag gtaaaaatca aaatcgccga
    15481 ataacgtcat ttcataggtt tttccggcaa ttttgtgata ttccaccccg tctgtcataa
    15541 tttgttgtaa agcaaggttg atttgctctg caattaaatc aataaagcgt tgcggattgc
    15601 ggaagacttc ttgcaagcgg tcggattttt gtaaaatttt gcaaagggtc gcacgcgtca
    15661 gcccggtttt cttttgaatc tcactcaata aatcaggtat cgcccattgt ttatttaccg
    15721 ctgtttcatt agatgagcga taattagttg aaatccctgt ttcattcatt tccaatttca
    15781 ctttggaatg cttaattttt gtcgcgtcga tatcgggcat tttgcggatt ttttccaccg
    15841 cactttcaat caattccgca ttactgaaat ctacccgata agtggtttgg tgtttaatcc
    15901 gttcccaaat tgccgcaaat tccggatgta agctgaaatc tttacggtag cgaacggctt
    15961 ttttatcttc ccgtttttta acattgcttt tggcaaactg tacaccacaa tcttcctcaa
    16021 tttcacgctg taatgtggcg gcaaattgtt cgtaactttc atttgcgata attgtcagcg
    16081 tatttagatg acgatcataa atccgcaatc cttgttgatt cacaggcaaa cgcaagcccc
    16141 gcccaatctc ttggcgtttt ttcatttcgc ttgcagtatc gtttaaagta caaatttgaa
    16201 acacattcgg attatcccat ccttctttta aggcagaatg cgagaaaata aaccgtaacg
    16261 gattctccag cgaaagcaat ttttctttat cccgcataat cagttgataa gtctcgttat
    16321 ccgcttgcgt tgtgccatta gtatcttttg ctacgccttt tttatcctgt gagaaataac
    16381 cgttatgcac gccttgaggc tcttctttgg ctagctccaa ataaatttcc tcaaaccatt
    16441 ttgcaaattt tccccccgta gggctttgtg cagaagggcg ataattatcc actttatcga
    16501 taaagaacaa agacaacacc ttgatcccaa gcggtcgtaa tttcttttct ttttgcaaat
    16561 gctcgccaat agtgcggcga atctgcattt tttgcacatc ttctttaagt tgggaatgtt
    16621 ccgccccttg aaaaatttgt gtgccgttgg agaagctaac catttggcta tcggtatcca
    16681 ttccgcttaa tataaaccct gaacgataca tttcattttg gttggaaagc tcatacaaat
    16741 cctgattcgg tttcacgcta atcactttct ttttcatcgc ttttttatcg ttaaatgaaa
    16801 tttcgatttt tgccgaccag ctttttgccc ctgttttaat ctctttgagg gaaagataag
    16861 ctccatttac ctcattttcc gccaatacgc tatctacctc aatttgttta actaatccca
    16921 tttcataggc ttgcaccgga ttgaggctaa aaatcgggtt atacgcattt ttatgggtgg
    16981 cagaataacg caaagtgaaa agcggactta aactctcaat cgcattacgg cgaatctccg
    17041 tttccatatt ttgcggttca tcaataatca caatcggatt cgcctgttga atatagaaaa
    17101 tcggagcatc accgctttca ttcacggtat taatcacgtt actttctttg gtgaaagaat
    17161 caatattcat cactaaaatt tcaatatgat tagcactagc aaaggatttt aagcgtgaaa
    17221 tttggttgct tttatattca aatttttgat tgacgctcgg gttatcaaat accgtattaa
    17281 aatggctttt tgtagtttcc aacgtatgca acactccctc acgaattgcc acactcggca
    17341 cgacaatcac aaatttttgc cagccgtatt gacggtttaa ttcaaaaatc gtgcgtaaat
    17401 agacataggt tttccctgta ccggtttcca tttccacggt aaaatttttt ccctgttcga
    17461 caagcaaagt gcggtcgatt ttttgcctgt tttgcagatt agccaaattc tgttgtaact
    17521 gctcatcact aatctgcaag cctaaattcg gcacaaaccg catatcattt tgggctttga
    17581 gtgaaaattc agttgcggca ttcggctgtc cgacaaacaa atttactgtg gcattaacgg
    17641 cttgtaattg atactctaag gtttcaaatt ggagttccat tttttctcct taaatcacaa
    17701 aaaacgtaat gccggcatct tgcatttgca attcggtgtt tttcttccgg gcatcattgc
    17761 cgttgaataa acggtctaaa gcgatgactt ttttcggttg ttgagcgata acagtttgaa
    17821 taatttcttc gtcggctgca tttaacagca aggcgaaaat cagcccgttt tcttccaccc
    17881 aaaacacctt attttccaac cgcacttttg ccgtgagttt taaccctaaa cgcaataaaa
    17941 gctcatataa aacggcttgc gtatccgctt tttcactaac aggatcaata aacatttcaa
    18001 gttgatcggc taaattttca gatgtgggcg attgccattg tttaaaatgg ctttctgtca
    18061 gtttgaagac tttaaaacct acatcaagcg gtcggttttc ggggttattt tgcaaaatct
    18121 gctttccggc acggcgaata cgttcttttg aaatttcagt tatatatagt ggtaaatttt
    18181 ttctttggca aaattgcttt gctacttttc cattcttact cttgatatca atttcttcag
    18241 gatattgaac cgcaataaat tttctattac ccttatcaat tctattactt tccaatattg
    18301 cgtgtggaaa tgatgaactg ccagcaaaga aatctaaaga aataaaattt ttatcttcaa
    18361 atagagttat taaatgtctt aaaaaattag gatttttagg tgcagagaaa acagggagtt
    18421 ttgtttcgaa taattctttt agcagtgttc ctgccgaagt tgtcttaaca tctagggtgt
    18481 aattggttaa attaccagcc gaaactgctt tatagttttt attattcagt
//
LOCUS       DANJWC010000033        18122 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.033, whole genome shotgun sequence.
ACCESSION   DANJWC010000033 DANJWC010000000
VERSION     DANJWC010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18122
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.033"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..674
                     /gene="malE"
                     /locus_tag="PWX02_000837"
     CDS             <1..674
                     /gene="malE"
                     /locus_tag="PWX02_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005537801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HDL1728869.1"
                     /translation="NLSEPYFTWPIAASNGAYAFKFANGKYDPKDIGVNNEGAVKALQ
                     FVVDMVKNKHISADMDYAVAEASFNKGQSALTINGPWSWGNIDKSGIKYGVTLLPTLN
                     GQASKPFVGVLSAGVNSSSPNKDLAKEFLENYLLTDDGLDAVNKDKPLGAVALKSYQE
                     KLAADPRIAATMANAKNGEIMPNIPQMSSFWYAEKSAINNAVTGRQSVKEALDDAHSR
                     IQKQQ"
     gene            793..2331
                     /gene="malF"
                     /locus_tag="PWX02_000838"
     CDS             793..2331
                     /gene="malF"
                     /locus_tag="PWX02_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HDL1728870.1"
                     /translation="MLTQTQSKSTHWLKYLLAGLVLLLDFYLVVLMYSQGEYLFAILT
                     LIILTSGVYIFTNKNTYAWRYVYPGITGMAIFILFPLVATIAIAFTNYSGSNQLSFER
                     AVSVLTEQRYFAGDKYQFTLYPQADNKYQIALTNPTTEQTFVSEPISLATGTNVVVTE
                     QNRPTGEVAPLKVITQNRAALQSMKVVLPNNNELTMSSLRQFSEQKPRYQFDEENKIL
                     LNNETGKRYQANDETGFFQSIDENGHWQSETLEPGYTVTSGFNNFIKIFTDEGIQKPF
                     VQIFIWTVIFSLLTVVFTVILGMVLACLVQWEALKGKAVYRVLLILPYAVPSFISILI
                     FKGLFNQSFGEINMILNQLFGISPEWFNDPLLAKVMILIVNTWLGYPYMMILCMGLLK
                     AIPADLYEASAMDGASTWQNFSKITFPLLLKPLTPLMIASFAFNFNNFVLIQLLTNGR
                     PDMIGTTTPAGYTDLLVSYTYRIAFEGSGTQDFGLAAAIATIIFLLVGGLALLNIKAT
                     KMEL"
     gene            2384..3274
                     /gene="malG"
                     /locus_tag="PWX02_000839"
     CDS             2384..3274
                     /gene="malG"
                     /locus_tag="PWX02_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HDL1728871.1"
                     /translation="MAIVQSKSVRYRVWATHFILICFLALIIFPLLMVIGISLRPGNL
                     AIGDIIPSQISWEHWQAALGFDVTHADGTVTPPPFPVLRWLWNSIKVATITSIGIVTL
                     STTCAYAFARMKFKGKKTILQGMLIFQMFPAVLSLVALYALFDRLGQYVPFLGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDGSLEEAASLDGATPWQAFRLILLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLYPQNYLWGDFAAAAVLSAIPITL
                     VFLLAQRWLIGGLTAGGVKG"
     gene            3349..5406
                     /locus_tag="PWX02_000840"
     CDS             3349..5406
                     /locus_tag="PWX02_000840"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDL1728872.1"
                     /translation="MKNALPYLCLFYTSSLFAQSWQHSHFSDFNDNLEKRLYQSQVQL
                     EKGSYPLRFKFGEQCYQPQQAIKLNQSVALVPCINEAPQLRLFRQGNYLAQIDLRSGT
                     PTLTISVEQQLQHNEAMFQSCPNWDKKPIEIDVSSTFAEGESVSDFYSGNTAIVKNGK
                     VTLMPNENASGLILLEKSNTENTATFDWKNATVYFVLTDRFYNGDPTNDHSYHRQKDG
                     MQEIGTFHGGDLKGLTEKLDYLQQLGVNVLWISSPLEQMHGWVGGGDKGDFPHYGYHG
                     YYHLDWTKLDANMGTEDDLSRFVQQAHQRGIRVLFDVVMNHTGYATLADMQEFNFGGF
                     YLTPDEISRTLGEKWTHWKPKSGQNWHSFNDFIRFNDNQAWQNWWGKEWVRADIADYD
                     SPKFDDLKMSLAALPDLKTESEQAVKLPEFFANKNTNAKTLPNAKVRDYLIGWLSDWV
                     RKYGIDGFRVDTAKHVEKSTWLVLKQSAQQALNEWQKANPNAGFNDRFWMTGEAWGHG
                     VFKSDYYQNGFDAMINFDFQDQAKQGLNCFAHIEPIYAEMNRKLSDFNVLSYLSSHDT
                     RLFFHSDSEQNIEKHKTAANLLLLSPGAVQIYYGDESGRTFGATGSDLIQGTRSDMNW
                     QELQQDPQKQALHQHWQKLTQFRQRHPAIGAGVHQLIKNDSYFAFKRVLNEDKVMVVW
                     AGN"
     gene            complement(5520..8246)
                     /gene="malT"
                     /locus_tag="PWX02_000841"
     CDS             complement(5520..8246)
                     /gene="malT"
                     /locus_tag="PWX02_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HDL1728873.1"
                     /translation="MLIPSKLVCSFRLQNSIPRKRLIQELDKSCSYPVVLINAPAGYG
                     KTTLVSQWIENKKNIGWYALDEGDNNSDRFATYFSAALHSAINEEIDVLLEENRKANL
                     LALFNQLLIKAAAFSEHFYLAIDDYHIIENDEIHEALKYWIKHQPSNMTLILISRSIP
                     PLSIASLRVQEQLLEIDINQLMFDHQESTAFFQARLGSELNQQDVIALCNEVEGWPTA
                     LQLISLFAKNKSHTLQVPLQDIAKRLAKSNHFHINEYLADEVLNKVDEETRLFILRCS
                     VLHSINESLVQAVTGEQNSRTKLESLEKQGLFLQQMANSKWQSSDDSWWKFHPLFASF
                     LNLCCQNELYAELPQLHQKAANAWLQLGYVTEALHHAMQLSDTGLLLNILSKHAWTVF
                     HQGELKLLEESLNCLDYAHLTEHTNLVLLKAWLVQSQHRHIEVGGILADFSRALTENK
                     IELSKTANAEFNVLRAQVAINSGDENTALQLASEALNDLSENAYYAHIVATSIIGEAH
                     HCHGNLAEALAMLQKAERMARQHHTYHNILWSQLQQSEILLAQGFSQAAYEMLDKASE
                     FVKENHLQKVPMYEFLLRSKGKILWEWYNLDKAESMAVAGMNALSKFEDKLQCLTLLT
                     KISLARGNLDNTARLLGEVEQLEHSHSYHHDWIANADQVRIFYWQMTNDVSAVRNWLI
                     QNPSPVSDKNHFTQVQWRNIARSRILLEEYHEAKAILDNLIETAEKFSLISDLNRALI
                     VRNRLYFLQGEKELAQQDLIQALKLTRQTNFISAFVIEGDVMAQQIRHLLQLNVLDEL
                     VLHKAQFILRNINQFYRQKFAHFDENFVNQLLKNPKVPELLKISPLTQREWQVLGLIY
                     SGYSNEQISDELQVAATTIKTHIRNLYQKIGVTNRNEAISYTKELLMLMGYH"
     gene            8410..10779
                     /gene="malP"
                     /locus_tag="PWX02_000842"
     CDS             8410..10779
                     /gene="malP"
                     /locus_tag="PWX02_000842"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HDL1728874.1"
                     /translation="MTFNSIVEKYCRYFDVTDPKHFTLQQWYQVVAEGSLELIYSQPA
                     TKPTESRHVNYLSMEFLIGRLTGNNLMNLGYYEQIRDYLKQYQVELVDVLEQERDPAL
                     GNGGLGRLAACFLDSMATVGQNATGYGLHYQYGLFKQSFKEGMQKEAADTWARDSYPW
                     HRFNPSKTRHIGFGGKIKHIQADQYEWQPKLTIQGKAFDLPIVGYKNNIIQPLRLWQA
                     DSDQSFDFDAFNEGKFLSADKTIVNAAALTQVLYPNDNHQAGQKLRLMQQYFHCACSV
                     ADILDRHISEGYSLEDLAKRQVIQLNDTHPTLAIPELMRVLLDEHQFTWEQAWDICSN
                     TFAYTNHTLLPEALEQWDQRLFKQLLPRHYQIVEKINDIFHDKVQAEFGDDAKIWEKL
                     AVLFDYRVRMANLCVVTCFRVNGVAEIHSDLLVTDLFPEYHKLFPTKFCNVTNGITPR
                     RWIRQANPQLSDLLDRTLKQDWTHNLELLKGVEQYADDTGFREEYQRIKRNNKVVLAN
                     EIHKTLGLNVNPNAIFDVQIKRFHEYKRQHLNLLNIIATYQSLKANPNQDYTPRLFLF
                     SGKAAPGYYLAKNIIHAINNVADIINNDKQVNDRLQVAFLPDYRVSLAEKIIPAADVS
                     EQISMAGKEASGTGNMKLALNGALTLGTLDGANVEIADMVGEENVFIFGHTVESVREL
                     LAKGYQPKAYYNQDPILKNAVDFLATGKASNGDKEAFKLMLDSLLERDPFLVFADFDS
                     YRQAQEKIGVAYLDQETWLRSAILNTARLGAFSSDRSIKDYQQRIWLNR"
     gene            10861..12939
                     /gene="malQ"
                     /locus_tag="PWX02_000843"
     CDS             10861..12939
                     /gene="malQ"
                     /locus_tag="PWX02_000843"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDL1728875.1"
                     /translation="MKITAKQYYRAGIMPYFFDERGVKKTASEKIKHSLFKAFNGNTQ
                     ARETPIPTVNIFYQHQPCFLPFHLADKKHPLTGTWQLQLENSNQIISGKIKARGITLP
                     QDLPLGYHQLQLKTVDKTFNCTIIIAPERCYQPQAVIEQKRLWGTFLQLYTLKSAHNW
                     GIGDFGDLKDFIQQIAPYQADFIGLNPIHSLFPANPNSASPYSPSSRRWLNIAYIDIN
                     QLAEFQQSDEAQTWFHSAEIQQQLSELRTQDWLNYEQIIPLKLKGLRFAFAKFQQNMT
                     ACAAPAFIEFVQKGGESLQVQATFDALHQHLSAQFADQWGWDFWEKAYQDYHSAAVQQ
                     FRETHKTDIEFYLWLQFVADQQLAECDAICRQQNMRIGMYRDLAVGVSPNGAETWNDK
                     QLYCLNASVGAPPDVLGPQGQNWGLTPMNPHVLKQQAYRPFIELVRANMSHCGALRLD
                     HIMSLLRLWWIPKGDSAVNGAYIRYPVDDLIAILALESQRHQCLIIGEDLGTVPKEIV
                     SKLKNAGILSYKIFYFEFDEQGKSRDLQHYPYQAMTTLSTHDLPTINGYWRGYDFELG
                     EKFGVYPNSDILAILKQDRVNAKAKILQRLQEHNVEIAPEISEQLNSPVNTAFIHCLQ
                     TYVANVSSGLFGFQPEDWLGMTEPVNIPGTSTQYANWRRRLSVNLEEIFTDKDIQALL
                     KEVNLIRKNQ"
     gene            complement(13004..14665)
                     /locus_tag="PWX02_000844"
     CDS             complement(13004..14665)
                     /locus_tag="PWX02_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HDL1728876.1"
                     /translation="MSEIALTVSLLSLVAVLGLWIGHIKIRGVGLGIGGVLFGGLFVS
                     HFMAQFDVKLDAHTLHFIQEFGLILFVYSIGIQVGPGFFASLKHSGLKLNGFAVLIVL
                     ISGILVILIHKFFNVPLPVILGIFSGAVTNTPSLGAGQQVLAELSAESVTEIMGMSYA
                     IAYPFGIIGILLSMWLIRILFRINIEAEAEAFNKQQNRGKDQLSTLNVRLTNPNIHGL
                     ALKQIPDFNLHEVAYSRLKRGNELSIPRVDTILQIGDILHLVGEESSLRKMQIILGEA
                     VDVSVSTKGTIYRSERAVVTNEKVFGKQIRQLMLKGKYDVVISRLNRAGVELVPTGDM
                     TLQFGDVLNLVGRQADIEMVMTIIGNAHSKLQQVQMLPIFIGIGLGVLLGSVPIYLPG
                     FPVALKLGLAGGPLVVALILARIGSFSKLYWFMPPSANLALREIGIVLFLAVVGLKSG
                     GRFLETLLSSEGVEWICYGAIITFVPLMIAGLIARMYNKVNYLTICGLLAGSMTDPPA
                     LAFANEIKEGNGAAALSYATVYPLVMFLRIMLPQVLAIMLWVSGG"
     gene            14883..16259
                     /locus_tag="PWX02_000845"
     CDS             14883..16259
                     /locus_tag="PWX02_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HDL1728877.1"
                     /translation="MLSNEVFISIIVLLILSLLRINVVIALIISALTCGLIGNLGVEE
                     AGFTEALEKTIKSFTGGLGGGAETAMNYAVLGAFAVALSKSGVTDLLAYKVIKSFGHS
                     PSGRSVTWFKYIVLFVLVAFAMSSQNLIPIHIAFIPILIPPLLSVMNKLQIDRRAVAC
                     ALTFGLTATYMLIPAGFGQIFIESILVKNINQAGEPFQLVTTTAEMTKAMLIPVSGMI
                     LGLLFAFFVSYRKPRVYIEDKPEPTSDDIEARVANVKPFHIGISILAIIATCSVQLAT
                     NSTIIGGLVGLAIFALGGVFRLKESNDVFQAGLRLMAMIGFVMIAASGFAGVVNSTGG
                     VPALVESLRDVISSKEMAALLMLVVGLFITMGIGSSFSTVPIITSIYVPLCVSFGFSP
                     LATMAIVGVAAALGDAGSPASDSTLGPTSGLNADGKHDHIWDSVVPTFIHFNLPLLVF
                     GWIAAMTL"
     gene            16360..17163
                     /gene="xthA"
                     /locus_tag="PWX02_000846"
     CDS             16360..17163
                     /gene="xthA"
                     /locus_tag="PWX02_000846"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDL1728878.1"
                     /translation="MKFISFNINGLRARPHQLEAIIEKHQPDVLGLQEIKVADEDFPY
                     DLVNHLGYHVFHHGQKGHYGVALLTKKEPIAVRKGFPTDVLDAQKRMIMADIETDFGV
                     LTVLNGYFPQGENRSHETKFPAKQKFYADLQNYLESELKPENPIIIMGDMNISPTDLD
                     IGIGEPNRKRWLRDGKCSFLPEEREWLDRLYSYGLVDTFRAGNPDVNDKFSWFDYRSK
                     GFDDNRGLRIDLILASKSLAERCVETGIDLDIRAMEKPSDHSPVWATFK"
     gene            17317..17901
                     /gene="lptC"
                     /locus_tag="PWX02_000847"
     CDS             17317..17901
                     /gene="lptC"
                     /locus_tag="PWX02_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HDL1728879.1"
                     /translation="MNKRLTVILLIIVAVLGGWYYSLQEKENTGLDQLIKKEGQPEYV
                     GNKMSTTVYDLKGNPQYFAQATEIKRYESTERTEFFKPFIELFAKESTQKQWKVSADY
                     AEITKEKMLNLKGNIKLVALDPASRLQQITTDTLTVDLNTQDIFTESTVKSIGSGFTT
                     TGVGLKGNLKQQVATLQKDVKSYIEPTIIQETKE"
     gene            17925..>18122
                     /locus_tag="PWX02_000848"
     CDS             17925..>18122
                     /locus_tag="PWX02_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HDL1728880.1"
                     /translation="MKLMFKSILVSVLLGSSFSAYALKGDTDQPINIDSGSQSLDMTN
                     NIVTFSDNVVITQGSIKVTAAN"
BASE COUNT         5519 a         3757 c         3512 g         5334 t
ORIGIN      
        1 ggaacttatc cgagccctat ttcacgtggc cgattgcggc ttcaaacgga gcttatgcat
       61 tcaaatttgc taacggcaaa tacgatccaa aagatattgg tgtgaacaat gaaggggcag
      121 taaaagcact acaatttgta gtcgatatgg tgaaaaataa acatattagt gcggatatgg
      181 actatgccgt tgccgaagcc tcctttaata aaggacaaag tgccttaacc atcaatggtc
      241 cttggtcttg gggcaatatt gataaaagcg gtattaaata tggtgtaact ttactcccaa
      301 cattaaacgg tcaagcttct aaaccattcg taggggtgtt aagcgccggt gtaaacagct
      361 caagcccaaa taaagattta gccaaagaat tcttagagaa ttatttactc accgacgacg
      421 gtttagatgc agtcaataaa gataaacctt taggtgcggt ggcgttaaaa tcttaccaag
      481 agaaattagc ggctgatcct cgtattgctg caacgatggc aaatgcgaaa aacggtgaaa
      541 ttatgccaaa tattccgcaa atgtcttctt tctggtatgc cgaaaaatct gctatcaata
      601 atgcagtgac cggtcgtcaa tcggtgaaag aagcattaga tgatgcacat tcacgcattc
      661 aaaaacaaca ataattcttc atcattctga gtgggttatt atgacccact ctatttttag
      721 attcacccta aatacaataa aagtacagta aaagtaccgt aacaaaatgc agtaaaaatt
      781 cgaggtgctg ttatgctaac tcaaactcaa tccaaatcca ctcattggct caaatatctc
      841 ttagccggat tagtcttgct ccttgacttc tatcttgttg tcttaatgta ctcacaaggt
      901 gaatatcttt ttgctatttt aacgctgatt attctaactt ccggtgtcta tatttttacc
      961 aataaaaata cctatgcttg gcggtatgtt taccctggca ttaccggaat ggcaattttt
     1021 attttgtttc cgctggttgc gactattgca attgcgttta ccaactatag tggttctaat
     1081 caattatcct ttgaacgagc ggtatccgtc ttaactgaac aacgctattt tgccggagat
     1141 aaatatcaat tcacccttta tccgcaagca gataataaat atcaaattgc attaaccaat
     1201 ccgacgacag agcaaacttt tgtgtcagag ccgatttcat tggctaccgg aacgaatgtg
     1261 gttgtaaccg agcaaaacag accaaccggc gaagtcgctc cccttaaagt gattacgcaa
     1321 aatcgggccg cacttcaatc aatgaaagtg gttttaccga ataataatga actgacaatg
     1381 agctcattac gtcaattctc cgagcaaaaa ccacgctatc aatttgatga agaaaataaa
     1441 atattactca ataatgagac tggcaaacgt tatcaagcaa atgacgaaac cggtttcttc
     1501 caatcgattg atgaaaacgg tcattggcaa agcgaaacac ttgaaccagg ttatactgtc
     1561 acatcaggct ttaataactt cattaaaatt tttaccgatg aaggtattca aaaacctttt
     1621 gtccaaatat ttatttggac ggtgatcttc tctttattaa ccgttgtctt taccgttatt
     1681 ctcggtatgg ttcttgcctg tttggtacaa tgggaggcat taaaaggtaa agcagtctat
     1741 cgtgtcttat tgattttacc ttatgccgta ccatcattta tttcgattct gatttttaaa
     1801 ggactattta accaaagttt tggtgagatc aatatgattt taaaccaact attcggcatc
     1861 agccctgaat ggtttaacga tccgctcctt gccaaagtga tgatcttaat tgtcaatacg
     1921 tggttaggtt atccttatat gatgatttta tgtatggggt tactcaaagc tattccggct
     1981 gatttatatg aagcctccgc aatggacggt gcttccactt ggcaaaactt cagtaaaatc
     2041 actttcccat tactgttaaa accacttact ccgttaatga ttgcttcgtt tgcatttaac
     2101 tttaataact ttgtattaat tcaactatta acaaacggtc gcccggatat gattggaaca
     2161 accactccag ccggttatac cgatttatta gtcagctaca cctatcgtat tgcctttgaa
     2221 ggaagtggta cgcaagattt cggcttagca gccgccatcg caaccattat tttcttatta
     2281 gtcggtggct tggcactctt aaatatcaaa gcaaccaaaa tggaattata aaagcgggag
     2341 ggatttaaca atcccgaccc caaactaact ttaggagtta tatatggcaa ttgttcaatc
     2401 taaatctgtg cgttatcggg tatgggcaac ccatttcatc ttaatctgtt tccttgcctt
     2461 aattattttc cctttattaa tggtgattgg catttcgtta cgaccgggaa atctggctat
     2521 cggtgatatt attccaagcc aaatttcgtg ggaacattgg caagccgctc ttggttttga
     2581 tgttacacac gccgatggta cagtcacacc gccgccattc ccggtgctac gctggttatg
     2641 gaactccatc aaagtggcaa caattacctc tatcggcatt gtaaccttat ctacgacctg
     2701 tgcttatgca tttgcccgaa tgaaatttaa aggcaaaaaa accattctac aaggtatgct
     2761 gattttccaa atgttcccgg cagtgctttc cttagttgcc ttatatgcgt tgtttgatcg
     2821 tcttgggcag tatgtaccgt ttcttggttt aaacacacac ggtggtgtta tttttgccta
     2881 cttaggcgga attgccttac acgtttggac aatcaaaggc tactttgaaa cgattgatgg
     2941 ttcattggaa gaagccgctt cccttgatgg tgcaaccccg tggcaagcgt tccgattaat
     3001 cttattaccg ctttcagtgc cgattttggc ggtagttttc attctttcat ttattgccgc
     3061 cattaccgaa gtaccggttg cctcactcct attgcgtgat gtaaacagct acaccctagc
     3121 cgttggtatg caacaatacc tctatccgca aaactattta tggggtgact tcgcagcagc
     3181 ggcggtctta tccgcaattc cgattacgct ggtcttctta ttggcacaac gctggttaat
     3241 cggtggttta actgccggcg gcgtgaaagg ctagtttgca aaaaattctc taaaaacgac
     3301 cgcttgtcga gctgatactg cggtcgtcaa tctttattag ggctgtctat gaaaaatgcg
     3361 ttaccttatc tctgcttatt ctatacctca agcctttttg cccaatcttg gcaacattct
     3421 catttttctg attttaacga taatctcgaa aaacggttgt atcaatctca agttcaattg
     3481 gaaaaaggga gctatccgct taggttcaaa tttggcgaac aatgttatca accccaacaa
     3541 gcgataaaat taaatcaatc ggttgcgtta gtcccttgta taaatgaagc tccacaactg
     3601 cggttgtttc gtcaaggcaa ctatctggca caaattgatc tgcgaagcgg cactccgaca
     3661 ctcacaatca gcgtagaaca gcagctacag cacaatgaag cgatgtttca aagttgcccg
     3721 aattgggata aaaaaccgat tgaaattgat gtttcttcca cttttgccga aggcgaatcg
     3781 gttagcgatt tttattcagg caacaccgct atcgttaaaa acggcaaagt caccttaatg
     3841 cccaatgaaa acgcaagcgg tctgattttg ctggaaaaaa gcaataccga aaatacggca
     3901 acgtttgatt ggaaaaatgc caccgtctat tttgtgctga ccgaccgctt ttataacggc
     3961 gatccgacca atgaccacag ctaccaccgc caaaaagacg gaatgcaaga aatcggcacc
     4021 tttcacggtg gtgatttaaa aggattaacc gaaaagctcg attacctaca acaacttggg
     4081 gtaaacgtat tgtggattag ctctccgtta gaacaaatgc acggctgggt tggcggcggt
     4141 gataaggggg attttccgca ttatggctat cacggttact atcatttaga ttggacgaaa
     4201 ttagatgcca atatgggtac tgaagacgat ttaagtcgct ttgttcaaca agcccaccag
     4261 cgtggtattc gggtgctatt tgatgtggtg atgaaccata ccggctacgc gacgcttgcc
     4321 gatatgcagg agtttaactt tggcggattt tacttaacac cggacgaaat tagccgcaca
     4381 ttaggcgaaa aatggacaca ctggaagccg aaatcaggac aaaattggca cagtttcaat
     4441 gatttcattc gctttaacga taatcaggct tggcaaaatt ggtggggaaa agaatgggtt
     4501 agagctgata ttgccgatta cgacagccct aaatttgatg atttaaaaat gtcgctcgct
     4561 gcccttcctg atctaaaaac cgagagcgaa caagcggtca aattacctga attttttgca
     4621 aataaaaata ccaatgccaa aacattacca aacgccaaag tgcgtgatta tttaattggt
     4681 tggttatccg attgggtcag aaaatatggc attgacggtt ttcgggttga taccgccaaa
     4741 cacgttgaaa aatccacgtg gctggtatta aaacaatcgg cacaacaagc cttaaacgaa
     4801 tggcaaaaag ccaatccgaa cgccggtttt aacgatcgct tttggatgac aggagaagcg
     4861 tggggacacg gcgtgttcaa aagtgactat tatcaaaacg gttttgatgc aatgatcaat
     4921 ttcgattttc aagatcaggc taaacaaggg ttaaattgct ttgcccatat tgagccgatt
     4981 tatgccgaaa tgaatcgcaa attaagcgac ttcaatgtgt tgagctattt atcttcacac
     5041 gatactcgat tatttttcca ttcggatagt gaacaaaata tcgaaaaaca caaaaccgcc
     5101 gcaaatttgc tgttgctttc acccggtgca gtgcaaatct attatggtga tgaaagtggg
     5161 cggacatttg gtgcgaccgg ttcagacctg atacaaggca cccggtcgga tatgaattgg
     5221 caagagttgc agcaagatcc tcagaaacag gcattgcatc aacattggca aaaattgaca
     5281 caattccgcc aacgccatcc ggctattggc gcaggtgtcc atcaactcat caaaaatgat
     5341 agctactttg catttaagcg tgtgttgaat gaagataaag tgatggtggt ttgggccgga
     5401 aattaaatct actttggctt caagttttct ctcttctggg gaaaacttga agccggttcg
     5461 gggaaacaag ttgcaaaaaa ttactaaaaa tagaccgctt gttccccatg agcttgtgat
     5521 caatgatacc ccatcaacat cagcagttct ttcgtatagc tgattgcttc gttacgattc
     5581 gtcacaccga ttttctgata aagatttcgg atatgggttt taatcgtggt tgcagccact
     5641 tgcaattcat ctgaaatttg ttcattgcta tatccggagt aaatcaaacc caatacttgc
     5701 cattcccgct gagtgagcgg actaattttt aagagttccg gcactttcgg atttttcaat
     5761 aattgattca caaagttttc atcaaaatgg gcaaattttt ggcgataaaa ttgattgatg
     5821 ttgcgtaaaa taaactgtgc tttgtgcaat accagttcat ctagcacatt aagttgcaat
     5881 aaatggcgga tctgctgtgc cattacatcc ccttcaatca caaacgcact gataaaattc
     5941 gtttggcggg tgagttttag tgcctgaatt aaatcttgct gagccaattc tttctcgcct
     6001 tgtaaaaaat aaagccggtt acgaacaatt aatgcgcgat ttaaatcgct aatcagagaa
     6061 aatttttcag ccgtttcaat taaattatct aaaatggctt tcgcctcgtg atattcctca
     6121 agcaagattc gagaccgtgc aatatttcgc cattgcactt gggtaaaatg atttttatct
     6181 gaaaccggtg aggggttttg aattaaccag ttcctcaccg cagaaacgtc attggtcatc
     6241 tgccaataaa atatacgtac ctgatcggcg ttggcaatcc aatcgtggtg ataagaatga
     6301 gaatgttcta attgttcgac ctcgcctaat aatcgggcag tgttatccaa attgcctcga
     6361 gccaacgaga tcttcgtgag taaagttaag cattgtaact tatcttcaaa tttagataag
     6421 gcattcattc cggcaactgc cattgattca gctttatcta agttatacca ttcccataaa
     6481 atcttgcctt tagaacgtag caaaaattca tacatcggca ctttttgtag atggttttct
     6541 ttcacaaact cgcttgcttt atctaacatt tcataggctg cttgtgaaaa cccttgggca
     6601 agcaaaattt cagattgttg aagctgtgac cacaaaatat tgtggtaagt atgatgctga
     6661 cgtgccatac gctctgcttt ttgcaacatt gctaatgctt ctgccagatt gccgtggcaa
     6721 tgatgagctt cgccaataat agacgttgca acaatatgag cataataggc attttctgaa
     6781 agatcgttta acgcttcaga cgctaattga agtgcggtat tctcatcacc ggaattgata
     6841 gcaacttgtg cccgtagcac attaaattcc gcattggcag ttttgctcaa ttcaatttta
     6901 ttttcagtta atgcacgaga aaaatccgct aaaataccac ctacttcaat atggcggtgc
     6961 tggctttgta ccaaccaggc tttgagtaac accaaattag tatgttcggt taaatgggcg
     7021 taatctaagc aatttaagct ctcttccaat aatttgagtt ctccttgatg aaacaccgtc
     7081 caagcgtgtt tgctcaaaat attgagtagt aatccggtat ccgataactg cattgcgtga
     7141 tgtaaggctt ctgtaacata gcctaattgt aaccacgcat tcgccgcctt ttgatgcagt
     7201 tgcggtaatt cagcatataa ctcattttgg caacataaat ttaaaaagga agcaaaaagc
     7261 ggatggaatt tccaccagct atcatcacta ctctgccact tactgtttgc catttgttgt
     7321 aaaaacaaac cttgcttttc gagagactct aatttggtgc gactgttttg ctcccctgta
     7381 acagcctgca ctaaagactc atttattgaa tgcaaaaccg aacaacgcaa aataaacaaa
     7441 cgagtctctt catcgacttt attaagcact tcatcggcta aatattcgtt aatatgaaaa
     7501 tggtttgatt tggctaaacg tttggcaata tcttgcagtg gtacttgtag cgtgtgtgat
     7561 ttattttttg caaaaaggct aattagctga agggctgtgg gccacccttc aacttcgtta
     7621 cacagtgcga taacatcttg ctgatttaac tccgaaccta atcttgcttg aaaaaaagcg
     7681 gtactttctt ggtgatcaaa cattaactgg ttaatatcaa tttccaataa ttgttcttgc
     7741 actcttaaac tggcaatact taacggggga attgatcgag aaatcaagat aagcgtcata
     7801 ttagacggtt gatgtttaat ccaatatttt aatgcttcgt gaatttcatc attttcaata
     7861 atatgataat catcaatagc aagataaaaa tgctctgaaa atgccgcggc tttaatcaat
     7921 aattgattaa ataaagctaa taaattagct ttacgatttt cctctaacaa tacgtctatt
     7981 tcttcattga ttgctgaatg caatgccgca ctaaaataag tggcaaaacg atctgaatta
     8041 ttatcgcctt catctaacgc ataccagccg atattctttt tattttcaat ccattgggaa
     8101 accagagtgg ttttgccata acctgccggt gcatttatca atacgaccgg ataactgcaa
     8161 gatttgtcta attcttgaat taaacgtttt cgaggaatgc tgttttgtaa tcgaaaagaa
     8221 caaacaagtt ttgatggtat taacataata aattcaattt tctctcataa caaaataggg
     8281 atgatatatc caccatttgt gttctcacta cttccttata atcataccat acaagatgat
     8341 tattgggatg atttgatttt atttggattt tacataatag cgacattcat ctttaaccgg
     8401 gggtttccta tgacatttaa ttctattgta gaaaaatact gtcgctactt tgatgtgaca
     8461 gatccaaaac actttacctt acagcagtgg tatcaagttg ttgctgaagg ttcccttgag
     8521 ctaatttata gccagcctgc aaccaaaccg acagaatcac gccacgtgaa ctacctctca
     8581 atggagttct taatcggtcg gttaaccggc aataatttaa tgaatttagg ttattacgaa
     8641 caaattcgtg actatcttaa acaatatcaa gttgaattag tagatgtttt agaacaagag
     8701 cgtgatccgg cactgggtaa tggcggatta ggtcgtcttg ctgcctgctt tttagattcg
     8761 atggcaactg tcggacaaaa tgcaaccggc tatgggcttc attatcaata tggtctgttt
     8821 aaacaaagct tcaaagaggg aatgcagaaa gaagcagcgg atacttgggc tcgtgacagc
     8881 tacccgtggc atcgttttaa tccgtctaaa acccgccata tcgggtttgg tggaaaaatc
     8941 aaacatatcc aagccgatca atatgaatgg cagcctaaat tgaccattca aggcaaagcc
     9001 tttgacttgc cgattgtagg ttataaaaac aacatcattc aaccattacg tttatggcaa
     9061 gcagatagtg accaatcttt tgattttgat gcctttaatg agggtaaatt tttaagtgcc
     9121 gataaaacga ttgtcaatgc agccgcacta actcaagtgc tgtatccaaa cgacaaccat
     9181 caagccggac aaaaattacg cttaatgcag caatacttcc attgtgcctg ctcggttgcc
     9241 gatattttag atcgtcacat tagcgaaggt tattcgttag aagatttagc aaaacgccaa
     9301 gttattcaac tcaatgatac ccatccaacg ctcgctatac ctgaattgat gcgtgtactg
     9361 ttagatgaac atcaattcac ttgggagcag gcttgggata tttgcagcaa tacctttgct
     9421 tacactaacc ataccttatt acctgaagca cttgaacaat gggatcaacg cctatttaag
     9481 cagctattac ctcgccatta tcaaattgtg gaaaaaatca atgatatttt ccacgacaaa
     9541 gtacaggcag aatttggtga tgatgcaaaa atttgggaaa aattagccgt gctatttgat
     9601 taccgtgtgc gtatggcaaa cctctgcgtt gtcacctgct tccgtgtaaa cggcgttgcg
     9661 gaaattcact ccgatctctt agtgaccgat ctattcccgg aataccacaa attattccca
     9721 acaaaattct gcaatgttac taacggcatc acacctcgcc gctggattcg acaagctaat
     9781 ccacaattaa gtgatttatt ggatcgtacc ttaaaacaag attggacaca caatctggaa
     9841 ttattaaaag gcgttgaaca atatgcggat gacaccggtt tccgtgaaga atatcaacgc
     9901 attaaacgca ataacaaagt ggttttagcc aatgaaatcc ataaaacatt agggttaaat
     9961 gtcaatccta atgcgatttt tgatgtgcaa attaagcgtt tccacgaata taaacgccaa
    10021 catttgaatt tgttaaacat tattgcgacc tatcaatcgc taaaagctaa cccgaaccaa
    10081 gactacacac cacgtctatt cctattctcg ggcaaagctg caccgggtta ttatttagca
    10141 aaaaatatta ttcacgctat taataatgtg gcagatatta ttaataacga caagcaggta
    10201 aatgatcgac tacaagtggc attcttaccg gattatcgag tcagtcttgc ggaaaaaatt
    10261 attccagcag ctgatgtatc agaacagatt tcaatggcag gtaaagaagc ctccggtacc
    10321 ggtaatatga aattggcact caacggcgca ttaaccttag gtacgctaga cggtgccaat
    10381 gttgaaattg ctgatatggt cggtgaggag aatgtcttta tcttcggtca caccgtagaa
    10441 agcgtgaggg aattattagc aaaaggctac caaccgaaag cctactataa ccaagatccg
    10501 attctgaaaa atgcggttga tttcttagcc acaggaaaag cctctaacgg agataaagag
    10561 gcattcaagc tgatgttaga tagcctgttg gaacgagatc ctttcctagt ctttgccgac
    10621 tttgatagct atcgccaagc acaagagaaa attggtgtag cctatttaga tcaagaaact
    10681 tggctgcgta gtgcaatttt aaatactgct cgtttgggtg catttagctc agaccgctca
    10741 attaaagatt atcaacaacg tatttggttg aatagataat aaattcgggc gacttctaca
    10801 ataaagaaag tcgcctattt tctgctgtat ttcatatgta tttatatatg gagagaaatg
    10861 atgaaaataa ctgccaaaca atattatcgg gctggaatta tgccctattt tttcgatgag
    10921 cgtggtgtaa aaaaaacagc atctgaaaaa ataaaacact cgttattcaa agcctttaac
    10981 ggcaatactc aagcaagaga aacacctatc cctacggtca acatttttta ccagcaccaa
    11041 ccctgttttt taccttttca tttagcggat aaaaaacatc cgttaaccgg tacttggcag
    11101 ctacaactag aaaattcaaa ccaaattatt tccggcaaaa ttaaagcaag gggaattaca
    11161 ttacctcaag atttgccctt aggttatcac caattacagc taaaaacggt agataaaacc
    11221 tttaattgca ccattattat cgctccggaa cgctgctacc aaccacaagc cgtcattgaa
    11281 caaaaaagat tgtggggaac cttcctacaa ctatacacct taaaatcagc ccacaactgg
    11341 gggattggcg attttggcga tttaaaagac ttcattcaac agattgcccc ttaccaagca
    11401 gattttatcg gcttgaaccc gattcactca ctttttccgg caaacccgaa ttctgccagc
    11461 ccatacagtc catcctctcg ccgatggctg aatattgctt acattgatat caatcagcta
    11521 gcggaatttc aacaaagtga tgaagcacaa acttggtttc attctgctga aatccaacaa
    11581 caattatcag aattacgcac tcaggattgg ttgaattacg agcaaatcat tccgttgaaa
    11641 ttgaaagggc tacgctttgc tttcgcaaaa tttcagcaaa atatgaccgc ttgcgctgcc
    11701 cctgctttta tcgaatttgt acaaaaaggt ggtgaaagct tacaagttca agccacattt
    11761 gatgcccttc atcagcatct atcggcacaa tttgccgacc aatggggctg ggatttctgg
    11821 gagaaagcct accaagacta ccactccgca gccgtgcagc aatttagaga aacgcataaa
    11881 accgacatag aattttacct ttggttacaa ttcgttgccg atcaacagct agcagaatgt
    11941 gatgctatct gccgtcagca aaatatgcgt attggtatgt atcgcgatct cgccgttggc
    12001 gtcagcccga atggtgcaga aacgtggaat gacaaacaac tttattgctt aaatgcctct
    12061 gtcggcgctc cgcctgatgt attaggtccg caaggacaaa actggggctt aaccccaatg
    12121 aatccgcatg tattaaagca acaagcctac cgtccgttta ttgagttagt cagagctaat
    12181 atgagtcatt gcggtgcatt acgccttgac catattatgt ctttactccg tttatggtgg
    12241 atccccaaag gcgatagtgc ggttaatggt gcttatattc gttatccagt tgatgattta
    12301 atcgcaattt tggcacttga aagccaacgc caccaatgcc taattatcgg tgaagattta
    12361 ggtactgtgc caaaagaaat tgtgagcaag ctcaaaaatg cgggaatttt atcttacaaa
    12421 attttctatt ttgaatttga tgaacaaggg aagagtcgag acttacagca ttacccttac
    12481 caagcaatga ccacattaag cacccacgat ttaccgacta tcaatggtta ttggcgtggc
    12541 tatgattttg aattaggcga aaaattcggc gtctatccta attcggatat tttggcaatc
    12601 cttaagcaag atcgagtcaa tgccaaagcc aaaatcttac aacgcttaca agaacataac
    12661 gtagagatcg cccctgaaat tagcgagcaa cttaattctc cggtgaatac cgcatttatt
    12721 cactgtttac aaacctatgt tgcaaatgta agcagcggat tattcggctt ccaacccgaa
    12781 gactggttgg gtatgactga gccggttaac attccgggaa ccagtacaca atatgcaaac
    12841 tggcgacgtc ggcttagtgt taacctcgag gagatcttca cagataaaga tattcaagcc
    12901 ttattaaaag aagttaattt aatccgtaaa aatcaataaa agttagataa gaaaaaaggc
    12961 gactttccgt cgccttttgg gcttatatgc ttgatgtcta tctttatcca cctgaaaccc
    13021 acaacataat cgctagcact tgaggtagca taatacgcaa aaacattact agtgggtaaa
    13081 cagttgcata ggataacgct gcagcgccat ttccctcttt aatttcattg gcaaaagcaa
    13141 gcgctggagg atcagtcatt gagccggcaa gtaatccaca aatcgttaaa tagtttactt
    13201 tgttatacat tctggcaatc aatcctgcta tcatcaatgg aacaaaagta ataatcgccc
    13261 catagcatat ccactcaacg ccctcactgc ttaatagcgt ttcgagaaat ctaccgccag
    13321 attttaaacc aactaccgct aaaaacagta caataccgat ttcacgcaat gcaagattgg
    13381 cacttggtgg cataaaccag tagagtttac tgaaactacc aattcttgca agaataagag
    13441 caaccaccag tggtccacca gccaagccca gctttaacgc aacagggaaa cccggtaaat
    13501 aaatcggtac agaaccaagt aatactccca aaccgatccc gataaatatt ggcaacattt
    13561 ggacttgttg caactttgag tgcgcattgc cgataatcgt cattaccatt tcaatatcag
    13621 cttgccgccc tactaaattg agaacatcac caaattgtaa cgtcatatca cctgttggta
    13681 ctaattctac accggctcga tttaaacgag aaatgacaac atcgtatttg cctttaagca
    13741 ttagctgacg aatttgtttc ccgaagactt tttcattcgt cactacagca cgttcagaac
    13801 ggtaaatcgt gcctttggtt gagacagata catctactgc ctcacctaaa ataatctgca
    13861 tttttcgtaa tgaggattct tcacctacta agtgtaaaat atctcctatc tgtagaatcg
    13921 tatctacacg tggaatagaa agctcattcc cccgttttaa gcgtgaataa gctacttcgt
    13981 gcaaattaaa atcaggaatt tgtttcaacg ctaaaccgtg aatatttgga ttagtcagac
    14041 ggacatttaa ggtactaagt tgatctttac ctctattttg ctgtttgtta aaagcctctg
    14101 cttccgcctc aatattgatt cgaaataaaa tccgaatcag ccacattgaa agtagaattc
    14161 cgataatgcc gaacgggtaa gcaatcgcat aactcattcc cataatttca gttacactct
    14221 cagcgcttaa ctcagccaat acctgttgac ctgctcctaa agaaggggta ttggttacag
    14281 ccccggagaa aatgcctaaa atcacaggca aaggaacatt gaagaattta tgaataagaa
    14341 taactaaaat accgctaatc agtacaatca gtaccgcaaa accattgagt tttaagccag
    14401 aatgttttag tgaggcaaag aatccaggtc ctacttgaat tccaattgaa taaacaaata
    14461 aaatcagccc gaactcttga ataaaatgaa gcgtatgggc atcgagcttc acatcgaatt
    14521 gcgccataaa atgggagaca aacagcccac caaataacac tccccctata cctagcccca
    14581 cgcctctgat tttaatatgg ccaatccata agcctagaac ggccactaaa gataataaac
    14641 tgacagtaag tgcaatttcg ctcattacag cctctcttta atatgaaata cctcaaaaat
    14701 ggtatccaat tctaccaaat ctcaaaaacg aaaaatgaga tctatgtcaa aaaaataaaa
    14761 tattgatagt attactccaa gatgaacttc aaaaaagttc ctgaatttaa aaaaaattct
    14821 cgacaatact tttttttaca gtaaattaaa caattattta aataacctaa ggatcaaaca
    14881 caatgttatc aaacgaagtc tttatttcga ttatcgtgct gttaatccta agcttgctta
    14941 gaattaatgt agtaatcgca cttattattt ctgcccttac ttgcggtcta atcggtaatc
    15001 ttggtgtaga agaggctgga tttaccgaag cattggaaaa aacaataaaa agttttactg
    15061 gtggtttagg tgggggtgcg gaaactgcta tgaactatgc cgttcttggt gcgtttgccg
    15121 tagctctatc aaaatcaggc gtgaccgatt tattagccta taaagtgatc aaatcctttg
    15181 gtcacagccc gtctggtcgc tctgtcactt ggtttaaata catcgtatta tttgtgttag
    15241 tggcgtttgc gatgtcatca caaaacttaa tcccgattca tattgccttt attcctattt
    15301 taattccgcc actattaagc gtaatgaata aattgcaaat tgaccgccgt gcagtagcct
    15361 gtgctttaac ctttggttta actgctactt atatgttaat tccggcgggt ttcgggcaga
    15421 tctttattga aagcatctta gttaaaaata tcaaccaagc aggtgaaccg ttccaattag
    15481 tgactaccac agcggaaatg accaaagcga tgttaatccc tgtatcaggt atgatcttag
    15541 gtctattatt tgctttcttt gtgtcgtatc gcaaacctcg tgtctatatt gaagataaac
    15601 ctgagccaac ctcagatgat attgaagctc gtgtcgcaaa tgtcaaacca ttccatatcg
    15661 gcattagtat tttagccatt attgcaacct gttcagtgca attggcaaca aattcgacca
    15721 ttattggtgg tttagtcggg ctagctattt ttgccttagg tggtgtgttt agattgaaag
    15781 aaagtaacga tgtcttccaa gctggtttac gtttaatggc gatgattggt tttgtaatga
    15841 ttgctgcttc tggctttgcc ggtgtcgtca attcaaccgg tggcgtgcca gcgttggttg
    15901 aaagcctgcg tgatgtgatt tcatcaaaag aaatggctgc attgttaatg ttagtcgttg
    15961 gtttatttat tacgatgggg atcggctctt cattctccac agtgccgatc attacctcaa
    16021 tttatgtacc actttgtgtt tcatttggtt tttcgccact agcaacaatg gcgattgtcg
    16081 gtgttgctgc tgcacttggt gatgcaggtt ctccggcatc agattccaca ctaggtccaa
    16141 cctccggttt gaatgcagat ggtaaacacg atcacatttg ggattctgta gtaccaacct
    16201 ttatccactt taatcttcca ttattagtgt tcggttggat tgcagcaatg acactttaag
    16261 ctataatatg caaaaatttt gctaaaaatg accgcttgta gccattacaa gcggtctatt
    16321 ttcttcttta ttttgcaaac cctacgatta ggattatcaa tgaaatttat ttcttttaat
    16381 atcaacggat tacgagccag accacatcaa ctggaagcta ttatcgaaaa acaccagcct
    16441 gatgtactcg gtttacaaga aatcaaagtc gctgatgaag acttccctta cgatttagtc
    16501 aatcacttgg gctaccacgt tttccaccac gggcagaaag gtcattacgg cgtggcatta
    16561 ctgactaaaa aagagcctat agcggtgcgt aaaggcttcc cgacagatgt gcttgatgct
    16621 caaaaacgaa tgattatggc ggatatcgag accgatttcg gcgtgctaac cgtattaaac
    16681 ggctatttcc cacaggggga aaatcgcagc cacgaaacca aattcccggc aaaacaaaaa
    16741 ttctatgctg atttgcagaa ctacttggaa agcgaattaa agccggaaaa tccgattatt
    16801 attatgggcg atatgaacat cagcccgact gatttggata tcggcatcgg cgagccaaac
    16861 cgcaaacgct ggctgcgtga cggtaaatgt tcattcttac ccgaagagcg tgaatggcta
    16921 gatcgcttat acagctacgg cttggttgat actttccgtg caggcaaccc agacgtgaac
    16981 gataagttct cttggtttga ctaccgttca aaaggttttg atgataatcg tggcttgcgt
    17041 attgatttaa ttcttgcttc aaaatcactt gctgagcgtt gcgtggaaac aggcattgat
    17101 ttagacattc gagcaatgga aaaaccgtct gaccactccc ctgtttgggc aacctttaaa
    17161 taaagttgca aaaaaatctg agttttcaac cgcttgtcag tttttagccc tacaagcggt
    17221 tattttttct cacttttttg cactttttac gctgtactat atccctattc aaatctgtgc
    17281 tacaatcaaa cactattttt agattatttt tggattatga acaaacgttt aaccgttatt
    17341 ttactgatta ttgttgccgt acttggtggc tggtattaca gcttacaaga gaaagagaat
    17401 acggggcttg atcagcttat taaaaaggaa ggacagcctg aatatgtggg gaataaaatg
    17461 tccaccacgg tctatgatct aaaaggaaac ccacaatatt tcgctcaagc tactgaaatt
    17521 aaacgctatg agtctactga gcgtactgag ttttttaaac cttttattga gctttttgcc
    17581 aaagagtcga cgcaaaaaca gtggaaagtc agtgccgatt atgcagaaat taccaaagaa
    17641 aaaatgttaa atttaaaagg taatatcaaa cttgttgcat tagatcctgc atcacgtttg
    17701 caacaaatca ctaccgatac gttaactgtt gatttaaata cacaggatat ttttaccgaa
    17761 agtacggtca aatcgatagg ttccggtttt actacaacgg gggttggatt aaaaggtaac
    17821 ttaaaacaac aagttgcgac tttgcaaaaa gatgtaaaat cttacattga accgaccatt
    17881 attcaagaaa ccaaagaata atttgcttgg aacaaggaat aacaatgaaa ttaatgttta
    17941 aatctatttt agtgagcgtg ttactgggta gcagcttttc cgcttatgct ctaaaaggcg
    18001 ataccgatca gccgattaat attgattcag gtagccaatc gttagatatg actaacaata
    18061 tcgtgacctt tagcgataat gtcgttatca ctcaagggtc gattaaagtg accgcagcaa
    18121 ac
//
LOCUS       DANJWC010000034        17610 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.034, whole genome shotgun sequence.
ACCESSION   DANJWC010000034 DANJWC010000000
VERSION     DANJWC010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17610
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.034"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            9..1616
                     /gene="pckA"
                     /locus_tag="PWX02_000849"
     CDS             9..1616
                     /gene="pckA"
                     /locus_tag="PWX02_000849"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDL1728881.1"
                     /translation="MLNRIEQELADLGITNVKEIVRNPSYEQLFEEEMKSDLKGYEKG
                     VLTNLGAVAVDTGIFTGRSPKDKYIVLDETTKDTVWWTSDAAKNDNKPMTQSTWQSLK
                     TLVTDELSNKRLFIIDAFCGASPEHRINVRIITQVAWQAHFVKNMFIRPTEEELKDFK
                     PDFVVMNGSQVTNPNWKEQGLNSENFVAFNITEGVQIIGGTWYGGEMKKGMFSMMNYF
                     LPLKGVASMHCSANVGEEGDVAVFFGLSGTGKTTLSTDPKRKLIGDDEHGWDENGVFN
                     YEGGCYAKTINLSIENEPDIYRAIRRDALLENVVVREDGTIDFDDKSKTENTRVSYPI
                     YHIDNIAVPSKAGHAKKVIFLTADAFGVLPPVSKLTPEQTKYYFLSGFTAKLAGTERG
                     ITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMEAAGAEAYLVNTGWNGTGKRISIKDT
                     RGIIDAILDGSIEKAEMSTLPIFNLAIPTALPGVETHILDPRNTYEDKAQWQAKAEDL
                     AGRFVKNFEKYATNEEGKALIAAGPKL"
     gene            1709..2710
                     /gene="bioB"
                     /locus_tag="PWX02_000850"
     CDS             1709..2710
                     /gene="bioB"
                     /locus_tag="PWX02_000850"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDL1728882.1"
                     /translation="MTTFQLRLKAKDELTPHPTAQYWRKCQVEELFDLPFMDLVFRAA
                     QVHRENFDANAIQLSTLLSIKTGGCPEDCEYCPQSARYDTGIEKQTMLDVETIVEKAK
                     IAKERGASRFCMGAAWRGPRPKDIKVVSEIINAVKSLGLETCGTFGMLEEGMAEELKQ
                     AGLDYYNHNLDTDPERYNKIIHTRNHEDRMDTLGKVRNAGLKVCCGGIVGMNETRPER
                     AGLIASLANLDPQPESVPINQLIKVEGTPLTNAEDLDWTEFVRTIAVARITMPQSFVR
                     LSAGRTTMPEAMQALCFMAGANSIFYGDKLLTTENPEEDHDRQLMDKLDLYPLQYIA"
     gene            2866..2963
                     /gene="ffs"
                     /locus_tag="PWX02_000851"
     ncRNA           2866..2963
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PWX02_000851"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2998..3705
                     /gene="gloB"
                     /locus_tag="PWX02_000852"
     CDS             2998..3705
                     /gene="gloB"
                     /locus_tag="PWX02_000852"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HDL1728883.1"
                     /translation="MLQITPIPALKDNYIWAIRNGQDVIIIDPSEHQPALNFIAKNAL
                     NLTAILITHNHHDHTDGVSGIVEQYPNIPVYGPQEVAEFANVIVSPEQHISLFGYDVR
                     IIESGGHTAGHISYLFGYDYLFCGDALFSGGCGRVFTGDYQAQFDALQRFKNLPNSVK
                     AYPAHEYTKSNLKFAEAVLPPSCVLSEYQEQVDLLRSQNKPTLPTTIGLEMQVNPFMQ
                     AVDLAEFIALRQQKDNF"
     gene            3864..4577
                     /gene="uppS"
                     /locus_tag="PWX02_000853"
     CDS             3864..4577
                     /gene="uppS"
                     /locus_tag="PWX02_000853"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005619022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HDL1728884.1"
                     /translation="MNFDPQNMPKHVAIIMDGNGRWAKQKGKLRIFGHQNGVKAVRSA
                     VSFAAKHHIQVLTLYAFSSENWSRPETEVSALMSLFMKALDSEVKKLHKNNIRLKIIG
                     DKSAFSENLQKRIAESEKLTENNTGLILNIAANYGGYWDIVQAAQQLAVHVKSEQLAV
                     EQITPALFQQHLVTENQPQVDLLIRTSGEQRISNFLLWQIAYAELFFSPVLWPDFNDE
                     AFSDAIYAYQQRERRFGGC"
     gene            4601..5470
                     /locus_tag="PWX02_000854"
     CDS             4601..5470
                     /locus_tag="PWX02_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDL1728885.1"
                     /translation="MLKERVLSAIIMIIAVLAAIFWLSPLPLTLVLSAIVVAAMWEWA
                     QFAGFKHPVPRAIVAMVTICLLLFAIVANTDYIRATRFITDETTPILFIGCIWWLIAL
                     VLVATYPNSAKLWEKSVAAKFLFGLATLVPFLIGTLALRFHNYAFDPYQGTYLFLYVC
                     LLVWGADSGAYFAGRAFGKRKLAPKVSPGKSWEGVIGGVLTSSLIAFTFLQLTPANVF
                     GKALATTPFILVSVGTVAISVLGDLAESMFKRQAGIKDSSQLIPGHGGILDRIDSLTA
                     AVPFFATFFFFVL"
     gene            5474..6784
                     /gene="rseP"
                     /locus_tag="PWX02_000855"
     CDS             5474..6784
                     /gene="rseP"
                     /locus_tag="PWX02_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDL1728886.1"
                     /translation="MTSIIAFFILICVLVFVHEYGHFWAARKCGVKVIRFSIGFGKVL
                     FKKKDKQGTEFVFSLIPLGGYVQMWNGETDINAPAQQALNTKSVLQRAFIIIAGPAAN
                     FIFAILAYWVVFIAGIPTVKPVIGEILPNTIASQARIPTELEITKIGHQSIQDWESAS
                     LALVGFVGNKNVPLEGNINGNSTQKYELDLSNWSIDSAKENPLTALGIRPKSAKVTAE
                     IKQVVENSPASQMGIQAGDRILQINQQPFNWFNLVELVQAGKPIELKIEQRGQIKDLV
                     VQPEKKDERYIIGVIPSYEPLADKYRTELKYDILTAFYKSIEKVWSLTQTILQFIGNL
                     ISGDLSIKNLGGPISMAKGAGATAEIGLVYYLSFMALISVNLGVMNLFPLLPLDGGQL
                     VLLAVEAIRGKALSEKIQLKFQQIGFAFVLSLMLFAFANDIIHF"
     gene            6899..9280
                     /gene="bamA"
                     /locus_tag="PWX02_000856"
     CDS             6899..9280
                     /gene="bamA"
                     /locus_tag="PWX02_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HDL1728887.1"
                     /translation="MKKLLLSSLLIANGVVAAPFVVKDIRVEGVQPTTGESIISSIPV
                     RVGQTATDTDVSNVVRHLFSQQRFADVRATREGNTLVIKVAEKPIIGKVEIEGNQAIP
                     KDALEQNLKANLINQGEIFDAAKLEAFKEGLIEHYHSTGRYEAKIDTSVANNTEGGVN
                     IKLAINEGEVAKAKTIKFEGNNAFSDKKLLENLDIQPDVSWWNIFESSKFEQTAYQKD
                     LETLRDFYLNRGYAKFAIQDTDVQFNDNKTEVALTYKLDEGSQYHVSEMRIIGNTANM
                     DSQLNALLKEFKPGQLFRKSDLTQIEEEIKSILGDAGYGSAKVDIYPQFNEENKTVKI
                     SFVVDAGRRLYVRKIRFEGNGVTADSTLRREMRQQEGAWLSTNKASLGKARLERTGFY
                     ESVDMSMPNVEGTADQVDVVYKIKERNTGSINFGIGYGTENGISYQAGIKQDNFLGMG
                     STISLNGTRNNYGTSVNLGYTEPYFTKDGVSLGGNIFYEDYDNSDNDSVASYKRQTYG
                     INGTLGFPVNENNSYYLGLGYTHDKIKNAAREYTREKYVKSMNFEFDPATDYYKKIKA
                     DDFDFSLGWNYNSLNRGYFPTEGTTANIGGKITIPGSDNKYYRVSADFRNYYPLNREH
                     KWVISTKASIAYTNGFGGKEVPFYQLYTAGGIGTLRGFSYGAIGPQAIYYNTKSGKFD
                     SMKNDIIGGNALAAASLELITPTPFVSEKYQHNVRTSLFVDAASAWHTKWKKDEYTNL
                     PDYGDFKRFRASAGIAFQWMSPIGPLSFSYAKPIRKYDNDDIEQFQFNIGSSF"
     gene            9360..10151
                     /locus_tag="PWX02_000857"
     CDS             9360..10151
                     /locus_tag="PWX02_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HDL1728888.1"
                     /translation="MKKLFKMTGLAVALASTTGIANAADTIGFVDPSYVLQNHPVLLD
                     ASAKFDKFMKESQEKFAADEKKLADENKTLTAERNKINEDAQKLQNEQKNVEASIKKK
                     VAALEKDAPRLRSKEIQARQNAIQKEANAFQAKVTAIQKREADFAKKAEAFQKRADEF
                     QKRIEQANKENGGINPQEAQKQAVDEVNATIKEIAKSKGYTLVLQPQVALYAEDEGKD
                     ITEAVLDAIVKKHPEIKIEKPATEAQPATEKETKAEETKPEEAKK"
     gene            10151..11176
                     /gene="lpxD"
                     /locus_tag="PWX02_000858"
     CDS             10151..11176
                     /gene="lpxD"
                     /locus_tag="PWX02_000858"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HDL1728889.1"
                     /translation="MANFRLIDLAEHIGGTLRGNADLAIQSIAALDKADSSQITFISN
                     AKYRENLTACNAGAIIVSPADVEFCRADQNLIIVANPYVAYAQLAQYMDSTPKAASEI
                     HPSAVISPEAKLGNNVSVGANAVIESGVELGDDAVIGAGCFVGKNSKIGARTQLWANV
                     SVYHNVQIGADCLIQSSTVIGSDGFGYANDKGQWIKIPQTGGVIIGNRVEIGACTCID
                     RGALDPTVIEDNVIIDNLCQIAHNVHIGFGTAVAGGVIMAGSLKVGRFCQIGGASVIN
                     GHMEICDGAIITGMGMVMRPITEKGIYSSGIPLQTNKEWRKTAALVMNIDEMNKRLKS
                     LEKRFND"
     gene            11205..11663
                     /gene="fabZ"
                     /locus_tag="PWX02_000859"
     CDS             11205..11663
                     /gene="fabZ"
                     /locus_tag="PWX02_000859"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL1728890.1"
                     /translation="MTTENREPNIIEVTEIMDMLPHRYPFLLVDRVTDYEEGKWLKAI
                     KNVTFNEPCFTGHFPGAPIFPGVLILEAMAQATGVLAVATYGKLSEDELYYFAAIDNA
                     RFKRPVVPGDQLQLEVEFLKEVRGITKFTGKAYVDGKLVCEADLMCARRK"
     gene            11697..12491
                     /gene="lpxA"
                     /locus_tag="PWX02_000860"
     CDS             11697..12491
                     /gene="lpxA"
                     /locus_tag="PWX02_000860"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HDL1728891.1"
                     /translation="MRLIDSTAKISPLAIIEEGAEIGAHVEIGPFSVIGKDVKIGART
                     KIHSNVVINGMTEIGEDNHIFQFASIGEINQDLKYQGEPTKVVIGNRNRIRESVTIHR
                     GTVQGGGVTKIGDDNLFMINTHIAHDCSIGNRCIIANNGTLAGHVTLDDFVIVGGMSA
                     IHQFVVIGSHVMLGGGSMVSQDVPPYVMAQGNHARPFGINFEGLKRRGFDKPTMHAIR
                     KVYKLIYSSGKTLEECLVEIEQIAATEPAIAIFKQFFKRSTRGIIR"
     gene            complement(12529..14529)
                     /gene="mnmC"
                     /locus_tag="PWX02_000861"
     CDS             complement(12529..14529)
                     /gene="mnmC"
                     /locus_tag="PWX02_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HDL1728892.1"
                     /translation="MGKLTFATLTFNDADTPVSEQFDDIYFSTQDGLAESYYVFQEGN
                     QLWEKWQSHPKEAFVIAETGFGTGLNFLAVADKFQQFRAAFPQSKLKRLYFISFEKFP
                     LTASQLADIHQRYPQFATLSENLTACWQPRQIGCQRYHFEQVYLDLWFGEIAENLPQL
                     GDLYSGTIDAWFLDGFSPDKNLEMWNEQLYLQMFRLSANGGSFATFTASSMVRRGLEA
                     AGFAVKKRKGFGKKREMLWGEKPLDSQVSEHNYPYFYAKNETADDIAIIGGGVASFFV
                     ALSLLEKGKQVTLYCKDETLAINASGNLQGAIYPQLSDDDERNVQFYIHSFDYALQRL
                     QQLKSNIEFEHNLSGVVLYAYNEKTAKKLEKMVEQTADDTLLKSCSQAELSEKLGLSV
                     PNSGAFIPQAGWLSPVQLVRNGFTWLEQKGLKIVLNHKVEHLTFSNGMWQWQTRGETV
                     RHQAVVLANGHTLNEFSQANGIPLYPVRGQVSQIPTTSELQKLKCVLCYDGYLTPMSA
                     YGTHCIGASHIRDNAETEFSLSEHWENVAKLQQNVTVCEWTQGIDLSENKAKVGIRAA
                     FRDRVPMVGAVHHFEQQKSQYANLYNQLIRKQAVENAEIFTNLYMVNGLSSRGLTTAP
                     LLGELLASLIVNEPVPISEDIWHNLSPNRAWIRKLLKGSTVA"
     gene            complement(14754..16190)
                     /gene="trpCF"
                     /locus_tag="PWX02_000862"
     CDS             complement(14754..16190)
                     /gene="trpCF"
                     /locus_tag="PWX02_000862"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HDL1728893.1"
                     /translation="MQNIVQDNAQPNQPTILQKIVQDKAIWVAQKEQAFPLSEFQHKI
                     VKSDHDFYAALAKGTHQEPAYILECKKASPSKGLIRAEFDLDSIAQVYKNYAAAISVL
                     TDEQYFQGDFRYIDQVKRQTTQPILCKDFMISAYQVYLARFYNADAILLMLSVVNDET
                     YRELATLAHELGMGVLTETSTEAEFERALALGAKVIGVNNRDLHTLTIDMNRIVRLVE
                     KYQAQIPADVCLISESGINDHQQVKNIKPYVHAFLIGSSLMGSADLNNAVRAVIFGEN
                     KVCGLTRVQDVKAVYEQGALYGGLIFAEGSPRQLSLRQAQELVVNAPLRYVGVFQNQA
                     VEFVEKMAKQLELFAVQLHGSEDEQYIAELAEKLGSKTQIWKALSVDVEVENLEFNDN
                     PQIARYVLDSKVGNQQGGTGKTFNWALIPETLKQKALLAGGIGTENIEQALAQGCVGL
                     DLNSGVESAKGVKDLQKLTACFGKIIQA"
     gene            16278..16589
                     /locus_tag="PWX02_000863"
     CDS             16278..16589
                     /locus_tag="PWX02_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HDL1728894.1"
                     /translation="MKSQVIQHLSYLEIALRVNQLWEATPPSPEALANEQPFCVETLT
                     PTQWLQWIFIPRMRAILDANADLPRNFAITPYLEEALKNEGYLQAICEPLLGLEKLLK
                     D"
     gene            16743..17480
                     /gene="truC"
                     /locus_tag="PWX02_000864"
     CDS             16743..17480
                     /gene="truC"
                     /locus_tag="PWX02_000864"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HDL1728895.1"
                     /translation="MTLDILYQDNSLIAINKPAGVLVHRSWLDKHETVFAMQTLRDQI
                     GQHVFPIHRLDRPTSGVLLFALNSETARIMSEQFEQHLVHLVQKTYLAVVRGYLQGSA
                     QIDYPLKVILDKIADKFSQEKEAQAAITDYKGLATVEMPYPVGKHQTSRYSLVELSPK
                     TGRKHQLRRHLKHLFHPIMGDSKYGDLHQNRALVEKSGVSRLMLHAHKLQFQHPKNLQ
                     KIEIIASLDEQWQRLFAFFDWNFTQYY"
     gene            17498..>17610
                     /locus_tag="PWX02_000865"
     CDS             17498..>17610
                     /locus_tag="PWX02_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HDL1728896.1"
                     /translation="MDNSLLSLTHEQQQAAVEEIQKLMAQGISAGEAIQIIA"
BASE COUNT         5471 a         3538 c         3546 g         5055 t
ORIGIN      
        1 taaccactat gttaaatcgt attgaacaag aacttgcaga cttaggcatt acaaatgtaa
       61 aagaaatcgt gcgtaaccct agctatgagc aactgtttga agaagagatg aaatcggatc
      121 ttaaaggtta tgaaaaaggc gtcctaacaa atttaggcgc agtagcggta gataccggca
      181 ttttcacggg gcgttcgccg aaagataaat atatcgtatt agatgaaacg acaaaagata
      241 ccgtctggtg gacatctgat gcagctaaaa atgataataa accgatgact caatctactt
      301 ggcaaagttt aaaaacctta gtgaccgatg agttatctaa taagcgttta tttattattg
      361 atgctttctg tggtgcaagc cctgagcatc gcatcaatgt gcgtattatt acccaagttg
      421 cgtggcaagc tcactttgtg aaaaatatgt ttattcgtcc aacagaagaa gaattaaaag
      481 atttcaaacc ggattttgtg gtaatgaacg gttcccaagt aactaacccg aactggaaag
      541 agcaaggctt aaattcagaa aactttgtgg catttaatat cacagaaggt gttcagatca
      601 ttggcggtac ttggtacggt ggtgagatga aaaaaggtat gttctctatg atgaactact
      661 tcttaccatt aaaaggtgtc gcttcaatgc actgttcagc caatgtgggc gaagaaggtg
      721 atgtggcggt tttctttggt ttatcaggaa ccggtaaaac gacactatca accgatccga
      781 aacgcaaatt gattggtgac gatgaacacg gttgggacga aaacggcgta tttaactatg
      841 aaggtggttg ctatgcgaaa accatcaacc tttctattga aaacgagccg gatatttatc
      901 gtgcaatccg ccgtgatgcg ttattagaaa acgtggttgt gcgtgaagat ggcactattg
      961 attttgatga taaatcaaaa acagaaaata cccgtgtgag ctatccgatt tatcatattg
     1021 ataatattgc cgtgccgtca aaagcaggac acgctaaaaa agtgattttc ttaacggcag
     1081 atgcattcgg cgtattgcca ccggtgtcta aattaacgcc tgaacaaact aaatactact
     1141 tcttatcagg ctttaccgca aaattagcag gtacagagcg tggtattact gagccaaccc
     1201 caactttctc tgcttgtttc ggtgcggcgt tcttatcact ccacccgaca caatatgctg
     1261 aagtgttagt gaaacgtatg gaagcagccg gtgcggaagc ctatttagtg aataccggtt
     1321 ggaacggcac aggtaaacgt atttcgatta aagatacacg gggtattatt gatgccatct
     1381 tagacggctc aattgagaaa gcggaaatga gtacattacc aatcttcaat ttagcaattc
     1441 caactgcgtt accgggtgta gaaacccaca ttttagaccc tcgtaacact tatgaagaca
     1501 aagcacaatg gcaagctaaa gcagaagatt tagctggcag atttgtgaaa aactttgaaa
     1561 aatatgcgac caacgaggaa ggcaaagcat taattgctgc tggtccgaag ttataagttg
     1621 taattataag ttataatcgc taaaaaatta gtttaggact aaaccttgaa tgaggtttag
     1681 tccttttgta taaaggagaa gtacaataat gacaacattc caattaaggc tgaaagccaa
     1741 agatgagctt accccacacc caacagcaca atattggcga aaatgccaag tggaagaact
     1801 atttgatctg ccgtttatgg atttagtgtt tagagcggca caagttcacc gtgaaaattt
     1861 tgatgctaat gcgatccagc tttcaacgtt actctcaatt aaaacaggag gctgtccgga
     1921 agattgcgaa tattgccccc aatctgcccg ctatgatacg ggaatagaaa agcaaacaat
     1981 gcttgatgta gagacgattg tagaaaaagc taaaattgcc aaagagcgtg gagcaagccg
     2041 cttttgtatg ggggctgcgt ggcgaggacc taggcctaaa gatattaaag tagtctcaga
     2101 aatcatcaat gcggtgaaat cattggggct agaaacctgt ggtactttcg gtatgcttga
     2161 agagggaatg gcagaagaat taaagcaagc aggactggat tactacaacc ataatttaga
     2221 taccgaccct gaacgctata acaaaattat ccatacccga aatcacgaag atcgaatgga
     2281 cactttaggt aaagttcgca atgccggctt gaaagtttgc tgtggcggta ttgtgggaat
     2341 gaacgaaacc cgccccgagc gagcaggcct aattgcaagc ctcgctaacc tcgatcctca
     2401 acctgaaagc gtaccgatta atcagttgat taaagtagaa ggtacaccgc ttaccaatgc
     2461 cgaagattta gactggacag aatttgttcg taccattgcg gtagcaagaa ttactatgcc
     2521 tcaaagcttt gtccgacttt ctgctggcag aactactatg ccggaggcaa tgcaagccct
     2581 ctgttttatg gcaggagcaa attctatttt ctatggcgat aaattactta ctacagaaaa
     2641 tccggaagaa gatcatgatc gccaattaat ggataaatta gatttatatc cgctacaata
     2701 tatagcctga gtgccaatga ttttcgcctg aaaattaaag aatcaagcgt tattttacgc
     2761 aaatttctaa cataaaataa ccgcttgcaa aattcgttct tgtcttattt ttaaatttat
     2821 ctataatgtg aggccttacg caagcttgcg taattcgatg gggcgttatt ggttcctcgc
     2881 aatggtgttc agcttacttg atcaggttcg gaagaaagca gtctgggttg aaatttccgt
     2941 gtgccgaggg tggctggtaa tgccccaccc ttttttcagt tctatcagga gaacgttatg
     3001 ctccagatca ctcccattcc ggcattaaaa gataattaca tttgggcaat ccgtaacggg
     3061 caagatgtga ttattattga tccatcagaa catcagccag cattaaactt tattgcaaaa
     3121 aatgcgttaa atttaaccgc tatcttgatt acccataatc accacgacca tactgatggg
     3181 gtgagtggta ttgttgagca atatccgaat atccctgttt atggtccgca agaagtggct
     3241 gaatttgcca atgtgattgt ctcgcctgaa caacatatca gcctgtttgg ttatgatgta
     3301 aggattattg aaagtggtgg gcataccgca gggcatatca gttatttatt tggctatgat
     3361 tatctgtttt gtggcgatgc gttattctcc ggcggttgcg ggcgggtttt caccggtgat
     3421 taccaagccc aatttgatgc gttacaacgc tttaaaaatc tgcctaactc ggttaaagcc
     3481 tacccggctc acgaatacac caagagcaat ttgaaatttg cagaagcggt tctaccacca
     3541 agctgtgtat tatcggaata tcaagaacag gttgatcttt tacgttcgca aaacaaaccg
     3601 actttgccaa ccactattgg cttggaaatg caggtgaacc cgtttatgca agcggtcgat
     3661 ttagccgaat ttattgcact tcgtcagcaa aaagacaatt tttaacctca tatttctaat
     3721 taagcattaa agcctctcac tttaatgctt tttggttaaa taaaaccttt gcaaacatcg
     3781 cttgtcaatt ccactctggc ttatcctatg gtatagttac gactattttt agcttaattt
     3841 attattttag tgagtaaaat tctgtgaact tcgatcctca aaatatgcct aagcacgttg
     3901 ccattattat ggatggcaat ggcagatggg ctaagcagaa aggaaaatta cgcatttttg
     3961 gtcatcagaa tggtgtaaag gcagttcgtt ctgcagttag ttttgcagca aagcatcata
     4021 tccaagtttt aaccctctac gcatttagca gtgagaattg gagcagacca gaaactgaag
     4081 tttcagcttt aatgtctctg tttatgaaag ctttagattc tgaagttaaa aaattacata
     4141 aaaataatat tcgcttaaag attataggcg ataaatccgc ctttagcgaa aatttacaaa
     4201 aacgcattgc cgaatcggaa aaattaaccg aaaacaatac tgggctaatc ttaaacattg
     4261 ctgccaacta tggtggttac tgggatattg ttcaagctgc tcaacaacta gcagtacacg
     4321 ttaaatctga acaacttgct gtggagcaga ttacaccagc attgtttcaa caacatttag
     4381 tgacagaaaa ccagccgcaa gtggatttat taattcgaac cagtggcgag cagcggatca
     4441 gcaattttct attatggcaa attgcttatg ccgagctgtt ttttagcccg gttttatggc
     4501 ctgattttaa tgatgaggct tttagtgatg caatttatgc ttatcagcaa cgtgaacgcc
     4561 gattcggtgg ttgttaatat aaaaagaata aggaaataca atgcttaaag aacgtgtact
     4621 ttcagcgatt attatgatta ttgccgtgct ggctgccata ttttggctct caccacttcc
     4681 tttaacttta gtgttatcgg caattgttgt ggctgcaatg tgggaatggg ctcaatttgc
     4741 cggctttaaa caccctgtgc cacgtgcaat tgtggcaatg gtgacaatct gcttattact
     4801 ttttgcgatc gttgcgaata ctgattatat ccgagccacc cgttttatta ctgatgagac
     4861 tacccctatc ctctttattg gctgtatttg gtggttaatt gcccttgtcc tagtggcaac
     4921 ttaccccaac tcggcaaaac tgtgggaaaa atctgttgca gctaaattcc tatttggact
     4981 tgctacctta gtgccgtttt taattggcac gctcgcattg cgtttccata attatgcatt
     5041 tgatccatac caaggaactt atttattcct ttacgtctgt ttattagttt ggggagctga
     5101 ttcaggtgct tattttgctg gcagagcctt tggcaaaaga aaacttgccc caaaagtatc
     5161 tccgggtaaa tcgtgggaag gcgtaattgg tggcgtactc acctctagtt tgattgcctt
     5221 taccttttta caacttacac cggcaaatgt attcggcaaa gcattagcca ctaccccatt
     5281 tattttagtt tcggtcggta cggttgctat ttcagtctta ggcgatttgg cagaaagtat
     5341 gtttaaacgc caagcaggca ttaaagacag tagccagtta attcctgggc atggaggtat
     5401 tttagatcgt attgatagtt taaccgctgc cgttccgttt tttgctacat tcttcttttt
     5461 tgtactatag aatatgacgt ccatcattgc attttttatt ttaatttgcg tactggtttt
     5521 tgtgcatgaa tacgggcact tttgggctgc tcgcaaatgc ggggtaaagg tgatccgttt
     5581 ttcgatcgga tttggtaaag tcttatttaa gaaaaaagat aaacaaggaa ccgaatttgt
     5641 tttttcactt attcctctgg gcggctatgt acaaatgtgg aatggtgaaa cggatatcaa
     5701 cgctccagct caacaagctc ttaacacaaa atctgtattg caacgtgcat ttattattat
     5761 agccggtcct gctgcaaatt ttatttttgc gattttggca tactgggtcg tttttattgc
     5821 cggtattcca actgttaaac ctgttattgg ggaaatttta ccaaacacta tcgcctccca
     5881 agcaagaatt cctaccgaat tggaaattac aaaaatcggt catcaatcta tccaagattg
     5941 ggaaagtgca tctttagcac tagttggttt tgtgggcaat aaaaatgtac cgttagaggg
     6001 aaatatcaac ggcaactcaa cacaaaaata tgaactcgat ttatctaact ggagtattga
     6061 ctcagccaaa gaaaacccat taaccgcatt aggtattcgc cccaaaagtg caaaagtcac
     6121 cgctgaaatt aaacaagttg tcgaaaattc tcctgcaagc caaatgggta ttcaagcagg
     6181 tgatcgtatt cttcaaatta accagcagcc atttaattgg ttcaatttag ttgaactggt
     6241 acaagcaggt aaacctattg aacttaaaat tgaacaacga ggtcagatta aagaccttgt
     6301 cgtccaacca gaaaaaaaag acgaacgcta tattatcggg gtaataccaa gttatgagcc
     6361 tcttgcagat aaatatcgta ccgaattaaa atatgatatt cttaccgcat tttataaaag
     6421 tatcgaaaaa gtgtggtctc ttacccaaac catactccaa tttatcggta atttaataag
     6481 cggtgatttg tccattaaaa atttaggtgg gccaatttca atggcaaaag gagccggtgc
     6541 aacagctgaa atcggcttag tttattacct tagttttatg gctctaatca gtgtcaattt
     6601 gggggtaatg aacttatttc cacttttacc cttagatggt gggcaacttg ttttattggc
     6661 tgttgaagca attcgtggaa aagcattatc agaaaaaata caactcaaat tccaacaaat
     6721 tggatttgca ttcgtgctga gtttaatgtt attcgccttt gccaacgata taattcactt
     6781 ttaacaagcg gttaaaaaag gtgacttttt tgcaaaaagc accctgacat caaaatccat
     6841 aatatggatt tacactatat tcttaataat tttgcaaaac gatcatagga taatttcaat
     6901 gaaaaaatta ttactttcct ctcttttaat tgcaaacggt gtagttgctg cacctttcgt
     6961 agtaaaagac attcgtgttg aaggtgtgca acctacaaca ggcgaatcta tcatttcatc
     7021 tattccggtt agagtaggtc aaacagcaac ggatactgac gtatctaatg tcgtccgcca
     7081 tctattcagc caacaacgtt ttgcagatgt tcgtgcaaca cgagaaggca atacgcttgt
     7141 catcaaagtc gctgaaaaac cgattattgg caaggtagag atcgaaggta accaagcgat
     7201 tcctaaagat gccttagagc aaaatttaaa ggcaaactta attaaccaag gtgaaatttt
     7261 tgatgctgcc aagcttgaag cttttaaaga aggactaatc gaacattatc actctaccgg
     7321 ccgttatgaa gccaaaattg acacatctgt tgcgaataac accgaaggtg gcgttaatat
     7381 taagttagcg attaatgaag gtgaagttgc taaagccaaa acaattaagt ttgaaggtaa
     7441 taatgccttc tctgataaaa aattattaga aaacttagat attcaacctg atgtttcttg
     7501 gtggaatatt tttgaaagta gcaaattcga acaaaccgcc tatcaaaaag acttagaaac
     7561 attaagagat ttttatttaa atcgtggcta tgcaaaattt gctatccaag ataccgatgt
     7621 tcaattcaat gataataaaa cggaagttgc attgacctat aaattagatg aagggtcaca
     7681 gtaccatgtc agtgaaatgc gcattatcgg caatacagcc aatatggata gccagctcaa
     7741 cgccctacta aaagaattca agcctggtca attattccgt aaatcagacc ttacacaaat
     7801 tgaagaagag attaaatcta ttttagggga cgcaggttat ggttcagcta aagtagatat
     7861 ttatcctcaa tttaatgaag aaaataaaac cgtaaaaatc agttttgttg tggatgccgg
     7921 tcgtcgtctt tatgttcgta aaatccgttt tgaaggtaat ggtgtcactg cagattcaac
     7981 attacgtcgt gaaatgcgcc aacaagaagg tgcttggctt tccacaaaca aagcttctct
     8041 aggtaaagct cgtttagaaa gaaccggttt ctatgaaagt gttgatatgt caatgccaaa
     8101 tgtagaaggt acagccgatc aagttgatgt agtgtataaa attaaagaac gcaataccgg
     8161 tagtattaac tttggtatcg ggtacggcac ggaaaacggt atcagctatc aagcaggaat
     8221 taaacaagat aatttcttag gtatggggtc aactatcagt ctaaatggta cccgtaataa
     8281 ctacggtacc agcgttaacc taggctatac cgaaccttac tttactaaag atggtgtaag
     8341 tttaggcggt aatattttct atgaagatta tgataactca gacaatgatt cagtggcttc
     8401 atataaacgc caaacctatg gtattaacgg cacattaggc ttcccggtaa atgaaaataa
     8461 ctcatactac ttaggtttag gctatacgca cgataaaatt aaaaatgcag cacgtgagta
     8521 cactcgtgag aaatatgtaa aatcaatgaa ttttgagttc gatccagcta ccgattatta
     8581 caaaaaaatc aaagcggacg attttgattt ttctctcggt tggaactaca acagcttgaa
     8641 tagaggctat ttcccgactg aaggaactac tgcaaatatt ggcggtaaaa ttacaattcc
     8701 aggctctgac aataaatatt accgagtcag tgcagacttt agaaattatt acccgttaaa
     8761 ccgtgagcac aaatgggtaa tttcaacgaa agctagcatt gcttatacta atggatttgg
     8821 tggtaaagaa gttccattct atcagcttta tactgccgga ggtattggta cactaagagg
     8881 tttctcttac ggtgctattg ggcctcaagc aatttactat aatacaaaat ccggtaaatt
     8941 tgatagcatg aagaatgata ttatcggtgg taatgcgtta gctgcggcaa gcttggaatt
     9001 aatcacacca acgccatttg tgagcgaaaa atatcagcac aatgtaagaa catctctctt
     9061 tgttgatgct gcaagtgcat ggcatacaaa atggaagaaa gatgaatata cgaacttgcc
     9121 tgactatggt gatttcaaac gtttccgtgc ttccgccggt attgcattcc aatggatgtc
     9181 tccaatcggg ccattatcat tctcttatgc gaaaccaatt cgtaaatatg ataatgatga
     9241 tattgaacaa ttccagttca acattggtag ttcattctaa ttaaccacaa gcggttaata
     9301 aatgtaaaaa atttgcaact tagccgctta tttcaaaact gttcatacaa ggaaaacaaa
     9361 tgaaaaaatt atttaaaatg actggcttag ccgtagcttt agcctctaca accggaatcg
     9421 cgaatgctgc agatactatt ggtttcgtag atccaagcta tgtattacaa aaccaccctg
     9481 tattattaga tgcttcggca aaatttgata aattcatgaa agaaagccaa gaaaaatttg
     9541 ctgctgatga aaaaaaatta gcagatgaaa ataaaacctt aacggctgag cgcaataaaa
     9601 ttaatgaaga tgcgcaaaaa ttacaaaatg aacaaaaaaa tgtagaagct tctatcaaga
     9661 aaaaagtggc agcattagag aaagatgcgc ctcgcttacg ttcaaaagag attcaagcac
     9721 gtcaaaatgc gattcaaaaa gaagcaaacg cattccaagc taaagtaaca gcaattcaaa
     9781 aacgtgaagc tgattttgca aaaaaagcag aagccttcca gaaacgtgca gatgaattcc
     9841 aaaaacgtat tgaacaagca aataaagaaa atggtggcat taacccacaa gaagcgcaaa
     9901 aacaagcggt tgatgaagta aatgcaacca ttaaagagat cgcaaaatcg aaaggttaca
     9961 ctttagtatt acaaccacaa gtagcacttt atgctgaaga tgaaggtaaa gatattactg
    10021 aagcagtact tgatgctatt gttaaaaaac atccggaaat taaaatagaa aaacctgcaa
    10081 ctgaagctca accagctaca gaaaaagaaa ccaaagctga agaaactaaa ccagaagaag
    10141 cgaaaaaata atggcaaatt tccgtttaat tgatttagcg gagcatatcg gcggtactct
    10201 taggggtaac gccgatttgg ctattcaaag tattgctgct ttagataaag ccgacagctc
    10261 gcaaattacc tttatctcaa atgcaaaata tcgggaaaat ttgaccgctt gcaatgctgg
    10321 agctattatc gtcagcccgg cagatgtaga attttgccga gcagatcaaa atcttatcat
    10381 cgttgccaat ccgtatgttg cttatgctca actggctcaa tatatggatt ctactccgaa
    10441 agcagcgagc gaaatccacc caagtgcggt gatttcacct gaagctaaac tgggtaataa
    10501 cgtatcggta ggggcaaatg ccgtgattga aagtggcgta gaattgggcg atgatgccgt
    10561 gatcggtgca ggctgttttg tgggaaaaaa cagtaaaatc ggggcaagaa cccaactttg
    10621 ggcaaatgtt tcggtgtacc acaatgtaca aatcggagct gattgcttga ttcaatcctc
    10681 aacggttatc gggagtgatg ggtttggcta tgcgaatgac aaagggcaat ggattaaaat
    10741 cccacaaacc ggtggtgtga ttatcggtaa tcgtgttgaa attggtgctt gcacttgtat
    10801 tgaccgaggt gcattagatc caaccgtaat cgaagataat gtgattattg ataacctttg
    10861 ccaaatcgcc cataatgtac acattggttt tggcactgcc gttgccggcg gcgtcattat
    10921 ggcaggcagt ctaaaagttg gtcgcttttg ccaaattggt ggagcaagtg ttatcaacgg
    10981 tcatatggaa atctgtgatg gtgccataat taccggaatg ggtatggtta tgcgcccaat
    11041 tacagaaaaa ggaatttact catcaggtat tccgctacaa accaataaag agtggcgtaa
    11101 aactgccgca ttagtgatga atattgatga aatgaataaa cgtttaaaat cacttgaaaa
    11161 acgttttaac gattaatttt attagaggtc ttaaagtgac agaaatgaca actgaaaatc
    11221 gtgagccaaa tattattgaa gtaaccgaaa ttatggatat gttaccgcat cgctaccctt
    11281 tcctgttagt tgatcgcgta accgattatg aagaagggaa atggttaaaa gccattaaaa
    11341 atgtaacctt caatgaacct tgttttaccg ggcattttcc tggcgcacca atcttcccag
    11401 gagtattgat tcttgaagca atggctcaag caactggcgt attagccgtt gccacttatg
    11461 gtaagttaag tgaagacgaa ctctactatt ttgccgcaat tgacaatgct cgctttaagc
    11521 gaccggttgt gccgggcgat caacttcaac ttgaggttga atttcttaaa gaagtacgtg
    11581 gtattactaa atttaccggt aaagcatacg ttgatggcaa attagtctgt gaagcagatt
    11641 taatgtgcgc tcgccgtaaa taattcgata taagcccaaa ctagagggaa caaactatgc
    11701 gtctaattga ttcaacagca aaaattagcc cgcttgccat cattgaagaa ggtgctgaaa
    11761 ttggcgctca cgttgaaatt ggtccatttt cagtcatcgg taaagatgtc aaaatcgggg
    11821 ctagaacgaa aattcactca aatgttgtca ttaacggcat gactgaaatt ggtgaagata
    11881 accatatttt ccaatttgcc agcattgggg aaatcaacca agatctaaaa taccaaggtg
    11941 aaccaaccaa agtggtgatc ggtaatcgta accgtattcg tgaaagtgtt actattcacc
    12001 gaggtacggt acaaggcggt ggcgtgacga aaatcggtga tgataactta tttatgatca
    12061 acacccacat cgcacacgac tgctctatcg gtaaccgttg tattatcgcc aataatggta
    12121 cgctagcagg tcacgttacc ctagatgatt tcgtgattgt gggaggaatg tccgccattc
    12181 accaattcgt tgtgattggt tcacacgtta tgcttggagg cggttcaatg gtcagccaag
    12241 acgtaccgcc ttatgtcatg gcacaaggta accacgcacg cccatttggt attaactttg
    12301 aagggttaaa acgtcgtggc tttgataaac caactatgca cgctattcgc aaagtctata
    12361 aactgattta ctccagcgga aaaacattgg aagagtgcct agtagaaatt gagcaaattg
    12421 cagcaacaga acctgcgatt gctattttta aacaattctt caaacgttct acccgtggta
    12481 ttattcgtta ataaacgaaa ccccgaaatc aattcggggt ttgttttatc aagcgaccgt
    12541 cgaacctttc aacaattttc gaatccaagc tcgatttggg ctaaggttat gccaaatatc
    12601 ctcactgatt ggcacaggct cattcacaat caggcttgct aacaattccc ctagaagtgg
    12661 tgcagtcgtc aaacctcgag aagacaggcc gtttaccata tacagattgg taaaaatttc
    12721 agcattttct accgcttgtt tccgtatcaa ctggttgtat aaattggcat attggctttt
    12781 ttgctgctca aaatgatgca ctgccccaac catcggtaca cgatcacgaa acgccgcacg
    12841 aataccgact ttggctttat tttcagataa atcaattcct tgcgtccact cacaaacggt
    12901 tacattttgc tgaagttttg caacattctc ccaatgctcc gataaactaa actcggtttc
    12961 tgcattatcc cgaatatggc ttgctccaat gcaatgcgtg ccgtaggctg acattggcgt
    13021 taaataacca tcataacaaa gcacacactt cagtttttgt aattcgcttg tagtcggaat
    13081 ttggctcact tgccctcgta ccggatacaa cggaatgccg ttcgcttggc taaattcatt
    13141 caacgtatgt ccatttgcca gcacaaccgc ttgatgtcga accgtttctc ccctcgtctg
    13201 ccattgccac attccattag aaaaggttaa atgctcaact ttgtgattca gtacaatctt
    13261 caaccctttc tgctccagcc aagtaaagcc atttctaaca agttgtaccg gtgaaagcca
    13321 accagcttgc ggaataaatg ccccgctgtt tggcacgctt aaacctaatt tttcacttaa
    13381 ttctgcctgt gaacaagatt ttaataaggt gtcatcagcc gtctgctcca ccattttttc
    13441 cagtttttta gccgttttct cgttataggc gtaaagcact accccgctta aattatgctc
    13501 aaactcaatg ttggatttca gttgttgcaa gcgttgcaat gcataatcaa aactatggat
    13561 ataaaactgt acatttcgct cgtcatcatc gcttaactgt gggtaaatcg ccccttgtaa
    13621 atttcctgaa gcatttattg ccaaggtttc atctttgcaa tacagcgtga cttgcttgcc
    13681 tttttctaac agcgataacg caacgaagaa actcgctact ccgccaccaa taattgcaat
    13741 gtcatcagcg gtttcatttt tggcataaaa ataggggtaa ttatgctcgg aaacttggct
    13801 atctaacggt ttctcgcccc aaagcatttc tcgttttttg ccaaatcctt tgcgtttttt
    13861 cactgcaaaa cctgcggctt ccagccctcg tctgaccata cttgaagcgg taaaagtagc
    13921 aaaactgccg ccattggcac ttagtcgaaa catttgcaga tataactgtt cgttccacat
    13981 ttctaggttt ttatcaggcg aaaagccatc taaaaaccaa gcgtcaatcg tgccgctata
    14041 aagatcgcct aattggggta agttttcagc aatctcaccg aaccataaat ctaaataaac
    14101 ctgctcaaaa tgataacgtt ggcaacctat ttggcgaggc tgccaacaag cggttaaatt
    14161 ttcagataat gttgcaaatt gcggatagcg ttggtgaata tcagctaatt ggctggccgt
    14221 cagaggaaat ttctcaaatg agataaaata gagtcgtttt agtttacttt gcggaaatgc
    14281 tgcccgaaac tgttggaatt tgtcagcaac ggctaaaaaa ttcaagcccg tgccaaagcc
    14341 ggtttccgca ataacaaaag cctctttcgg gtgagattgc cacttttccc acagttggtt
    14401 accttcttga aacacatagt aactttccgc caaaccatct tgggtagaaa aataaatatc
    14461 atcaaattgc tccgacaccg gtgtatctgc atcattaaat gtaagtgtcg caaaagtcag
    14521 tttacccatc atatcccctt aaaaacaaaa cacggaaaac cccaagattt tccgtgtttc
    14581 aaccagttca atagtaccaa tattagaaaa tgttgctggc aaaaataacg atcataccaa
    14641 gtgcgcctac aaccgttagc attgaataac caaaagtacc cattttacaa tcctcattgt
    14701 taattatgat taaaacggct ttattctagc ataatttcca tcgctttgaa tgcctatgct
    14761 tgaataattt tgccaaaaca agcggtcaat ttttgtaaat cttttacgcc tttggcactt
    14821 tccacgccag aatttaaatc taacccgaca cagccttgag caagggcttg ctcaatattc
    14881 tcagtgccaa tgccacctgc caataacgct ttttgtttta aggtttctgg aatcaacgcc
    14941 caattaaagg ttttgcctgt gccgccttgc tgattgccta ctttgctgtc taatacataa
    15001 cgagcaatct gtggattatc gttaaattct agattttcaa cctcaacatc aaccgataag
    15061 gctttccaga tttgagtttt actacctaat ttctctgcca gttctgcaat gtattgctca
    15121 tcttccgagc cgtgcaattg aactgcaaat aactcaagct gttttgccat tttttccaca
    15181 aattcgaccg cttgattttg gaacaccccc acataacgca acggtgcatt caccaccaat
    15241 tcttgggctt ggcgaagtga aagctggcga ggcgagcctt cggcaaaaat taaaccgcca
    15301 tataatgccc cttgctcata aaccgctttc acatcttgca cacgggttaa gccacacact
    15361 ttgttttcac caaaaatcac cgcccgcacc gcattattga gatcggcact tcccattaaa
    15421 ctgctgccga ttaaaaaagc atgaacatac ggcttaatgt ttttcacttg ctggtgatcg
    15481 ttaatacccg attctgaaat caaacagaca tcagccggaa tttgagcttg gtatttttcc
    15541 accaaacgca caatgcggtt catatcaatc gtgagcgtgt gcaaatcacg gttattaacg
    15601 ccaattacct tcgcccctaa ggctaaggca cgttcaaatt ccgcctccgt gctggtttca
    15661 gtcaaaacgc ccattcctaa ctcgtgagcc agtgtggcaa gttcacggta ggtttcatca
    15721 tttaccaccg ataacatcaa taaaatcgca tcagcattat aaaaacgggc aaggtaaacc
    15781 tggtatgccg aaatcataaa atctttacac aaaatcggct gtgtggtttg gcgtttgact
    15841 tgatcgatat aacggaaatc gccttgaaaa tattgctcat cggttaaaac cgaaatcgcc
    15901 gcagcgtaat ttttatacac ttgggcaata ctgtctaaat cgaactcagc acgaattaac
    15961 ccttttgacg gtgaagcttt tttacattct aaaatataag ctggttcttg gtgtgtgcct
    16021 ttggcaagag ccgcatagaa atcatggtcg ctttttacaa ttttgtgctg aaattcactt
    16081 aacggaaagg cttgctcttt ttgtgccacc caaatggctt tatcttgtac gatcttttgt
    16141 aaaatcgtcg gttggtttgg ttgagcgtta tcttgaacga tgttttgcat ataaattcct
    16201 agagcgaaat cggtttgaat ggtaaaatct gcccttattt ttacccgaaa aagtctcatt
    16261 tcacaagcaa aactaaaatg aaatcacaag ttatacagca tttatcttat ttagaaatcg
    16321 ccctgagagt taatcaactt tgggaggcga caccaccaag ccctgaagcc ttagccaatg
    16381 agcaaccttt ctgcgtggaa accttaaccc caacccaatg gctacaatgg atttttattc
    16441 ccagaatgcg ggccatttta gatgcaaatg ccgatttacc tcgcaatttt gcaatcaccc
    16501 cttatttaga agaggcattg aaaaacgaag gttatttaca agcgatttgt gaaccgctat
    16561 taggtttgga aaagttgttg aaagattaac ttctctctca gttcttttat taatctgaca
    16621 aattcgttga tagtgccagc gtccacgctg gtactaattt taacggttaa ttaatcaaaa
    16681 tttggcacaa gcgaggacgc ttgcgccatc atagagattt gataaattat acttaatgta
    16741 ttatgacctt agatatttta taccaagaca actcgcttat cgccatcaac aaacctgccg
    16801 gagtgttggt gcatcgttct tggttagata aacacgaaac ggtgtttgca atgcaaaccc
    16861 tgagggatca aatcgggcaa cacgtttttc cgattcaccg ccttgaccgc ccaacctcag
    16921 gtgtattgct gtttgctctc aacagcgaaa ccgcccgaat aatgagcgag cagtttgaac
    16981 aacacctagt acacctagta caaaaaactt atttggcggt ggtgcgtggc tatttacaag
    17041 gttcggcaca aattgattat ccgctcaaag tgattttaga taaaattgcg gataaatttt
    17101 ctcaggaaaa agaggctcaa gcggcgatta ctgattataa aggcttagca acggttgaaa
    17161 tgccttaccc tgtcggcaag caccaaacct ctcgttatag tttggttgaa cttagcccta
    17221 aaaccgggcg gaaacatcag cttcgccgcc acctgaaaca cctgttccac ccgattatgg
    17281 gagacagcaa atatggcgac ttacaccaaa accgtgcttt agttgaaaaa tcaggcgtaa
    17341 gtcgtttaat gttacacgcc cataaattgc aatttcagca cccgaaaaat ttgcaaaaaa
    17401 tagagataat tgcatcgctt gatgagcaat ggcaacgcct ctttgcattt tttgactgga
    17461 actttactca atattattag acaaaaaaga ggattgaatg gataattcac ttttatcatt
    17521 gacacacgag caacaacaag ccgcagtaga agaaattcaa aaactgatgg cacaaggcat
    17581 cagtgccgga gaggcaattc aaattattgc
//
LOCUS       DANJWC010000035        17114 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.035, whole genome shotgun sequence.
ACCESSION   DANJWC010000035 DANJWC010000000
VERSION     DANJWC010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17114
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.035"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            113..1288
                     /locus_tag="PWX02_000866"
     CDS             113..1288
                     /locus_tag="PWX02_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HDL1728897.1"
                     /translation="MKKTSLFSLFTFSLLSACSSLPTSGPSYSEVLASNENNAETQLP
                     EVNLIKLDNITVQNLYQAQQSQRFSGFDGSIGVGGYAGTVNVGDILEISIWEAPPAVL
                     FGGTFSAEGQGNGHLTQLPQQMVNKNGTVTVPFIGNIKVAGKTPEAIQNQIVGALSRK
                     ANQPQALVRIANNLSSDVTVIRQGNSIRMPLSANNERILDAVAAVGGTSENIEDVTVR
                     LTRGNKVRSMAFETLIADPSQNITLRSGDVVALMNTPYSFTGLGAVGNNQQMRFSSKG
                     ITLAEAIGKMGGLIDDRSDPRGVFVFRHIPFTQLDYDNQAQWREKGYAEGMDVPTVYQ
                     VNLLKPQSMFLLQRFPIQDKDIVYVSNAPLSEFQKFLRMIFSITTPITGTANTIRAY"
     gene            1360..2463
                     /locus_tag="PWX02_000867"
     CDS             1360..2463
                     /locus_tag="PWX02_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HDL1728898.1"
                     /translation="MSKKKKHFLANPLLWLTVIIPTACSGIYYSLFASDIYVSESSFV
                     VRSPNNQSSLTGVGALLQNSGFARSQDDTYSVQEYMRSRTALEQLEAEIPVKRFYSEQ
                     GDLLSKFNGFGFNDTQEAFYRYFKEHLNIDVDSISGIATLRVRAFNAEEGQKINENLL
                     QQGETLINRLNERARQDTVEFAEERVKEAEKDVNNTALALSQYRTKNKIYDLPAQSGV
                     QLSLISTLKSELIRVETQLAQLQSITPDNPQVAALQMRQKSLKKEIDDQSKQLAGGNT
                     SVATQTAEYQRLVLANELAQQQLSAAMTSLQNTRGEADRQQLYLEVIGKPSKPDWALE
                     PYRIYNIISTFFIGLILFGVLSLLIASIREHKN"
     gene            2463..3260
                     /locus_tag="PWX02_000868"
     CDS             2463..3260
                     /locus_tag="PWX02_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1728899.1"
                     /translation="MRYGDNTTFKRSCVIQKRVIHALLMRELITRYGRRNIGFLWLFI
                     EPLLLTLIISLAWKYIKADKYSTLNIVAFMLTGYPIAMMWRNTTNRSKGAISANISLL
                     YHRNVRVLDTIFTRALLEVAGTSIAQILLMAFFIAIGWIDVPHDIFYMLCAWVLMAFF
                     ALGLGLIICALAHYIEPFGRIWGTFSFVLLPLSGAFFFVHNLPSQAQQIALWFPMIHG
                     TEMFRHGYFGETVITYESISYIIVSDLILLFIGLLFVRSFSKGIEPR"
     gene            3257..3904
                     /locus_tag="PWX02_000869"
     CDS             3257..3904
                     /locus_tag="PWX02_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1728900.1"
                     /translation="MISIRNVSKRYRTNSGWRTVLKDINFELKKGEKVGILGRNGAGK
                     STLIRLMSGVEPPTTGTIERQMSISWPLAFSGAFQGSLTGMDNLRFVSRIYDADFDYV
                     KSFTEEFSELGEYLYEPVKSYSSGMKARLAFALSLSIEFDCYLIDEVIAVGDARFAAK
                     CKHELFEKRKDRSLILVSHSPASIKEYCDSAMVLEEGIMHTFDSIDNAYNFYNKT"
     gene            complement(3982..4611)
                     /locus_tag="PWX02_000870"
     CDS             complement(3982..4611)
                     /locus_tag="PWX02_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HDL1728901.1"
                     /translation="MDFSKILSQVLDTAKDAATNGLIKGNSKNDQIAKIGGGAVAIGL
                     ISTLFGRNGGSSLAKLGSLAAIGSLAYQAYQKYQAQNSSVSELSQNNFENVDENVASK
                     VILQAMIAAAASDGAITEEEKAAIVSQVGDEAEVIEWIRQEMDTPATVEEIARQVGND
                     QALATQVYLASRAVCRDLERKEIVFLANLADALGLAEALVEQLEKDAGF"
     gene            complement(4683..5156)
                     /locus_tag="PWX02_000871"
     CDS             complement(4683..5156)
                     /locus_tag="PWX02_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HDL1728902.1"
                     /translation="MLEKITACPCQTGKRYAECCQPFHLKQTFASTAEQLMRSRYSAY
                     TLVNIPYIVETTVPSQQKLLDQTAMKEWAENTKWVGLSVLNHLPSLSRIHSQVEFKAF
                     FDTDEGIQGHHETSLFVQIDERWFFVDPTVPLPHQKQPCVCGSGKKFKHCCGNYL"
     gene            5605..7497
                     /gene="mnmG"
                     /locus_tag="PWX02_000872"
     CDS             5605..7497
                     /gene="mnmG"
                     /locus_tag="PWX02_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDL1728903.1"
                     /translation="MIYNQIYDVIVVGGGHAGTEAALAPARMGLKTLILTHNVDTLGQ
                     MSCNPAIGGIGKGHLVKEIDAMGGLMAKATDVAGIQFRTLNNSKGPAVRATRAQADRV
                     LYRNAVRTALENQPNLDIFQQEVVDILVENNRAVGAVTKMGLTFKARAVVLTAGTFLA
                     GKIHIGLDNYAGGRAGDPAATMLADRLRDLNLRVDRLKTGTPPRLDARTINFDVLAKQ
                     HGDEVLPVFSFMGDVSQHPRQIPCYITHTNDQTHDLIRNSLDRSPMYTGIIEGVGPRY
                     CPSIEDKVMRFADRNSHQIYLEPEGLNTIEVYPNGISTSLPFDVQMGIVNSMKGLENT
                     RIIKPGYAIEYDYFDPRDLKPTLETKAIDGLFFAGQINGTTGYEEAGAQGLLAGINVA
                     LQVQGKEAWYPTRDQAYTGVLVDDLCTLGTKEPYRVFTSRAEYRLLLREDNADIRLTP
                     KAHELGLIDEARWVRFNQKMEAIEQERARLKSTWAHLQMPNLDELNALLANPLAREAS
                     GEDLIRRPEMSYEKLTQIALFAPAIEDKQAAEQVEIAIKYQGYIEHQYNEIERHKRHE
                     NTLIPVEFDYDKVESLSNEVRAKLMQHRPVSIGQASRISGITPAAISILLVNLKKQGM
                     LKRGEL"
     gene            7592..7816
                     /locus_tag="PWX02_000873"
     CDS             7592..7816
                     /locus_tag="PWX02_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728904.1"
                     /translation="MIYLRKLGFSPFDHQMFAQPSWYFCNALVHTNYENLRQGVFVTK
                     AYLVMFLENLLFEKSHSLSNRALRINENND"
     gene            7829..8443
                     /gene="rsmG"
                     /locus_tag="PWX02_000874"
     CDS             7829..8443
                     /gene="rsmG"
                     /locus_tag="PWX02_000874"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDL1728905.1"
                     /translation="MLAKLNRLLAQAEINLTDQQKEQLVGFVRLLDKWNKAYNLTSVR
                     NPDEMLVKHILDSLVVSRHLQGETFIDVGTGPGLPGIPLAIANPDKQFVLLDSLGKRI
                     TFIKNALRELGIKNVTPVQSRVEEYTESQFDGVLSRAFASLNDMIDWCYHLPAENGKF
                     YALKGVYDEQEITEIKQPIQLVEVIKLEVPELVGERHLVVLKKP"
     gene            8711..10073
                     /locus_tag="PWX02_000875"
                     /pseudo
     CDS             8711..10073
                     /locus_tag="PWX02_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248294.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            10096..11142
                     /locus_tag="PWX02_000876"
     CDS             10096..11142
                     /locus_tag="PWX02_000876"
                     /inference="COORDINATES: protein motif:HMM:NF016497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL1728906.1"
                     /translation="MQIQNPVLPGFNPDPSIVRVGDTYYIATSTFEWFPGVRIHESKD
                     LVHWNLVKNVLDTTTLLDMRGNPSSGGIWAPDLSYAEGKFWLVYTDVKITDGAFKDMT
                     NYLTTAKEIQGPWSAPIKLNGIGFDASLFHDDDGRKYLVQQTWDHREYHHPFDGITLT
                     EFDVATMKLKPETQRTIFEGTEVKLVEGPHIYKINGEYYLFAAEGGTVFTHQEVVARS
                     KTLEAHSFQLAPNTPFITNFDTPNSYLQKQGHGSLVDTPSGEWYYASLTARPWNHATE
                     SATDPRGWSTLGREAAIQKVEFDQDGWPYIVGGHGGKTFVEAPKDAIFTEAPIDHSQH
                     DEFDSSTLDINWNT"
     gene            11145..11783
                     /locus_tag="PWX02_000877"
     CDS             11145..11783
                     /locus_tag="PWX02_000877"
                     /inference="COORDINATES: protein motif:HMM:NF036826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,4-xylosidase"
                     /protein_id="HDL1728907.1"
                     /translation="MPFSKEMGEVGNGVLKLIGRGSLANTHDLSLIARRWQAFYFDAE
                     TKVKFTPFSYQSMAGLTNYYNHSHWSWIFITKNDQGQQVIEVAENKGGLRNGQYTSYL
                     KDKAIVIPDGTEYVWFKTKVRKETYRYEYSFDGKQWHTIPVELDAAVLSDDYVLRSYG
                     GFFTGAFVGLAAVDYSSYESTAEFSYFDYKELGDKQLTDGSFSWEKSESCFG"
     gene            11802..12782
                     /locus_tag="PWX02_000878"
     CDS             11802..12782
                     /locus_tag="PWX02_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL1728908.1"
                     /translation="MKQRRFGKTNKMVSEIGLGTWQLGTKWGEPFNHQEAMAILETAY
                     EQGINFIDTADVYNNGQSEKAIGDILKKYPDFFYVVTKCGRALNPHTAEMYTPQAIEK
                     FVDGSLSHLGTEKLDMILLHCPPTLVYRNDEIFTKLEQLKAGGKLIDYGVSIETVEEG
                     LLAMEYDIAAMEVIFNMFRLKPLEQLFPTAQQKNVGIIARVLLASGLLTGRYNACTQF
                     GKDDHRTFNRNGEAFDKGETFSGVDYQLGLQAVEELKALFGTEDLIPYALRWVLMNEA
                     VSTVIPGASKVSQVITNAAVDNFPPLTENEMQKVEDIYNCLIRPSVHHLW"
     gene            complement(12844..14184)
                     /locus_tag="PWX02_000879"
     CDS             complement(12844..14184)
                     /locus_tag="PWX02_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1728909.1"
                     /translation="MFSFLKASPPAEQKVEASRVDAEYRKLRWQVFAGVFIGYAAYYL
                     IRKNFSLAMPYLIDEYGFTKADLGTVGVALSLAYGFSKFIMGNVSDRSNPKYFITIGL
                     LGSAIVSLVFGLVPGVLSSIPIMIILAALNGWFQGMGYPPGAKTMTNWFSTSERGVWW
                     SWWNVSHNLGGGLIGPLAILGLSIFGVWQSLFYLPALIAIVLAFVMFYLMRDTPESQG
                     LPPIDEYKGEKVVEKVESAHTLSSKDIFYKYILNNKFLWAIAIANVFVYFIRYGIIDW
                     APTYLKEVKHFSVDKQSWAYFLYEYAGIFGMLASGYLSDKVFKGHRAPPMLLFLAGVL
                     IAIVVYWKNPAGNPLIDNICLMAIGFLIYGPVMMIGLQAADLVPRVATGTATGLTGLF
                     GYLLGSASAGYVMGKLVDMFGWDGGFYALILSCFLAFAFIALTLFKKTSKQLEV"
     gene            complement(14374..15447)
                     /gene="glpQ"
                     /locus_tag="PWX02_000880"
     CDS             complement(14374..15447)
                     /gene="glpQ"
                     /locus_tag="PWX02_000880"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL1728910.1"
                     /translation="MKFKFKTLVAALALSATALGAYAQQSDKLVIAHRGASGYLPEHT
                     LESKALAFGQQADYLEQDLAMTKDNRLIVIHDHFLDGLTDVAKKFPERKRADGRYYVV
                     DFTLDEIKTLEMTENFEVKDGKQAQVYPNRFPMWQSHFTIHTLEEELEFIQGLEKSTG
                     KKIGIYPEIKAPWLHHKEGKDIALETLKVLKKYGYDSKTSPIYLQTFDFNELKRIKTE
                     LLPKLGMDVKLVQLVAYTDWHETEEKDKNGNWVNYDYDWMFKDGAMAEVAKYADGVGP
                     GWYMLVDKEKSKVGNIVYTPLVKELAKYKMELHPYTVRKDALPDFFQNVDEMYDALLN
                     KAGATGVFTDFPDTGVEFLKKQK"
     gene            complement(15517..16962)
                     /gene="glpT"
                     /locus_tag="PWX02_000881"
     CDS             complement(15517..16962)
                     /gene="glpT"
                     /locus_tag="PWX02_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDL1728911.1"
                     /translation="MFGPFKPAPHIAELPADKVDARYKFLRWQVFAGIFFGYAAYYFV
                     RANFDLAQKGLIEAGMYTKTDLGVIGTAAGLAYGLSKFVMAGMSDRSNPKVFLPTGLL
                     LSGLCMTLMGLMPWATSSILVMWVMIFLNGWFQGMGWPPCGRTMVHWWSKSERGTIVS
                     IWNTAHNLGGMVPGAMVLLASALYFSEYGVEATAKDVWQQALYFPGIAAMIAAIPIYL
                     VMKDTPQSCGLPPVEKWRNDYPDDYNEKTYEHDLSTKEIFVTYVLKNKLLWYIAIANV
                     FVYLIRYGVLKWSPVYLGEVKHFNIKGTAWAYTIYELAAIPGTLLCGWVSDKLFKGKR
                     GLTGFIFMILTTAAVVAFWLNPATPESEIAQYAGHAWYENPYQLMDFIWMTTIGFLIY
                     GPVMLIGLHALELAPKKAAGTAAGFTGLFGYLGGTVAASAVVGWAADKFGWDGGFYVM
                     IGGGVLAILLTFIVMVEEGKHKAKLEDHYGK"
BASE COUNT         5094 a         3439 c         3525 g         5056 t
ORIGIN      
        1 aaatcaaata caaaaataca acctattgtt tctattaaat agtattgata agttaagcta
       61 cgcctagctt aacattagtg ctatactact atcaaatgta atgaggtatc taatgaaaaa
      121 aacatctttg tttagtctat tcacttttag cttactatca gcctgctcca gcctaccaac
      181 gtccggcccg agctatagcg aagtcttagc aagtaatgaa aataacgctg aaacacaatt
      241 acctgaagtc aatttaatca agttagataa catcaccgta caaaacttat accaagcgca
      301 acaatctcaa cgtttttcag gctttgatgg ctcaatcggt gtgggtggtt atgccggaac
      361 agttaatgta ggcgatatat tggaaatatc catttgggaa gcgccacctg ccgtgctatt
      421 tggtggaaca tttagtgcag aagggcaagg taatgggcat ttaactcaat taccacagca
      481 aatggtgaac aaaaatggta cggtaactgt gccttttatc ggtaacatta aagtggcagg
      541 gaaaacgcct gaagctattc aaaatcaaat tgttggtgca ttaagccgta aggccaatca
      601 gcctcaagca ttagttcgta ttgcgaataa cttgtcgtct gatgttactg ttatccgcca
      661 aggaaatagc attcgtatgc cattaagcgc aaataacgag cgcattttag atgccgttgc
      721 ggcagtaggt ggtacaagcg aaaatattga agatgtaacg gttcgtttaa cacgtggaaa
      781 taaagtacgt tcgatggcgt ttgaaacctt aattgccgat ccctcacaaa atattacatt
      841 acgttcaggc gatgttgttg ccttaatgaa tacgccttat agctttaccg gcttgggggc
      901 tgtgggaaat aatcagcaaa tgcgtttctc aagtaaaggg attacacttg ctgaagctat
      961 aggtaagatg gggggattaa ttgatgatcg ttcagaccct cgtggtgtgt tcgtattccg
     1021 ccatattcct tttacacaat tagattacga taaccaagct caatggcgtg aaaaaggtta
     1081 tgcagaggga atggacgttc caactgttta tcaggtgaat ttattaaaac cgcaatcaat
     1141 gtttttattg cagcgtttcc ctattcaaga taaagatatt gtatatgtgt cgaatgctcc
     1201 actgtctgaa tttcaaaaat tcttgagaat gattttctcc attactacac caattacagg
     1261 tacagctaat acaattagag cgtactaatt agctcataga taaaatggta aaaaatcatt
     1321 aaaaaatgac cgcttgtaac acttttcgtt gaggatttta tgtcaaagaa gaaaaaacat
     1381 tttttagcca accctttgtt gtggctaact gtaattattc caactgcttg ttccggaatt
     1441 tattattcct tatttgcctc tgatatttat gtttcagaat ctagctttgt ggttcgttca
     1501 ccgaataatc aatccagctt aaccggcgtg ggtgctttat tgcaaaactc cggtttcgcc
     1561 cgttctcaag atgataccta ttcagttcag gaatatatgc gttcccgtac cgcattagag
     1621 caacttgaag cagaaattcc ggtaaaacgt ttttatagtg agcaaggtga tttactaagc
     1681 aaatttaacg gtttcggatt taacgatacc caagaagcgt tttaccgtta ctttaaagaa
     1741 catctgaata ttgatgtcga ttctatttca ggtattgcga cgttacgcgt tcgtgctttt
     1801 aatgccgaag aagggcagaa aattaacgag aatttattac agcaaggaga aacattaatt
     1861 aaccgtctta atgaaagagc aagacaagat acggttgagt ttgcagaaga acgcgtaaaa
     1921 gaggccgaaa aagatgtcaa taatactgcg ttagcattaa gccaatatcg aaccaaaaat
     1981 aaaatttatg atttacctgc acaatccggc gttcagctct cattaatttc taccttaaaa
     2041 agtgaattaa ttcgggttga aacacaatta gcacaattgc agtcaattac gccggataat
     2101 cctcaggtag ccgcattaca aatgcgtcaa aaaagtttga aaaaagaaat tgatgaccaa
     2161 tctaaacaac ttgcaggagg taatacttct gtggcaacgc aaacagctga atatcagcgt
     2221 ttagttttag ctaacgagct agctcaacaa cagctttcag cggcaatgac atcgctacaa
     2281 aatacaagag gagaggctga tcgccagcaa ctttatttag aggtaattgg taaaccaagc
     2341 aaaccggatt gggcattaga accctacaga atttataata ttatatcaac tttttttatc
     2401 ggcttaattc tatttggagt attaagtttg ttaatagcaa gtatcagaga gcataaaaac
     2461 taatgcgata tggtgataat acaaccttta aacgttcctg cgtgattcaa aaacgagtga
     2521 ttcacgctct tttaatgcga gagttgatca cccgttatgg gcgtagaaat attggctttt
     2581 tatggctgtt tattgaaccc ttattattaa ctctaatcat ttcattagct tggaagtaca
     2641 ttaaagctga taaatattct acgcttaata ttgttgcatt tatgcttacc ggctatccga
     2701 ttgcaatgat gtggagaaat acaacaaata gatctaaggg agctatatcc gcaaatatta
     2761 gcctgcttta tcatcgaaac gttcgtgtat tagataccat attcactcga gctttattgg
     2821 aagttgcagg gacatctatt gctcaaatcc tattaatggc gttttttatc gctattggct
     2881 ggatagatgt accccacgat attttctata tgctttgtgc ttgggtatta atggcatttt
     2941 ttgcattagg tttaggctta attatctgtg cattagcaca ttatattgaa ccatttggca
     3001 gaatttgggg aacctttagc ttcgtattac ttccattgtc cggagcattc tttttcgtcc
     3061 ataacttacc atcacaagca cagcaaatcg cactgtggtt tccaatgatt cacggtacag
     3121 aaatgttcag acatggctat tttggcgaaa ctgtcattac ctatgaaagc attagttata
     3181 ttatagtcag cgatcttatt ctgttattca tcggcttact ctttgtgaga agttttagta
     3241 aaggaatcga acctagatga ttagtattag aaatgtcagt aagcgttata gaacaaatag
     3301 cggttggcgt accgttctta aagatattaa ctttgagcta aaaaaaggtg aaaaagtggg
     3361 tattttaggg cgcaatggtg caggaaaatc aaccttaatc cgtttaatga gcggagtaga
     3421 acctccaaca acaggtacga ttgaacgtca aatgtcgatt tcttggccac tggcatttag
     3481 tggtgcattc cagggtagtt tgaccggaat ggataatctt cgtttcgtca gccgaattta
     3541 tgatgctgat tttgactatg taaaatcttt caccgaagaa ttttcagaac tcggcgaata
     3601 tttatatgaa ccggtaaaaa gctattcttc tggtatgaag gcccgtttag cctttgcatt
     3661 atcgctttct attgaatttg attgttactt aattgatgaa gtgattgcag taggcgatgc
     3721 tcgattcgct gccaaatgta agcacgagct ttttgaaaaa cggaaagacc gttcattgat
     3781 tttagtttct catagcccag cctcaattaa agaatattgc gattctgcaa tggttttaga
     3841 agagggtatc atgcatactt ttgattctat agataacgct tataatttct acaataaaac
     3901 ataaaagaac aagcggttat atttcacaag tttttcgcaa aaaactctca aaatataacc
     3961 gcttgttact aaaactgaaa attagaaacc tgcgtctttt tctaattgtt caactaatgc
     4021 ctcagctaaa cctaaagcat cagctaaatt agctaagaaa acgatttctt tgcgttctaa
     4081 atctctgcat actgcacggg aagctaagta aacttgtgta gccaatgctt ggtcattacc
     4141 tacttggcgg gcaatctctt caactgtagc cggagtgtcc atttcttgac gaatccactc
     4201 aatgacttca gcctcatctc caacttggga aacaatagct gctttttcct cttcagtaat
     4261 agcaccatct gaagcagcag cggcaatcat tgcttgtaaa atcactttac ttgccacatt
     4321 ttcatctaca ttttcaaaat tattttggct aagttctgaa accgatgaat tttgcgcttg
     4381 atacttttgg tatgcttgat aagcaaggct accgatagcg gctaaagagc ctaattttgc
     4441 taaacttgaa ccgccattac gcccaaataa cgttgaaatt aaaccaatag cgacagcgcc
     4501 gccaccaatt ttagcgatct gatcgttttt agaattacct ttaattaatc cgtttgtagc
     4561 ggcatctttt gctgtatcta atacttgact taaaatctta ctgaaatcca tttttgttcc
     4621 tcttggtaag taaatgaaat ttactcatta gtccttagat tcaaacaaga gttcaattca
     4681 aattaaagat aatttccaca acagtgctta aacttctttc cggagccaca tacacaaggt
     4741 tgtttttgat gaggcaaagg tacggtagga tccacaaaaa accatcgctc atcaatttga
     4801 acaaaaagag aggtttcgtg gtgtccctgt atcccctcat ctgtatcaaa aaaggcttta
     4861 aattcaacct gactatgtat tctcgaaaga gaaggtaagt gatttagcac ggataatcct
     4921 acccattttg tattttcagc ccactctttc attgcagtct gatcaagtaa tttctgctga
     4981 cttggcactg tcgtttccac aatataggga atattaacca aagtataagc actgtaacgg
     5041 gagcgcatta attgctctgc cgttgatgca aatgtctgtt ttaaatggaa aggttggcaa
     5101 cattcagcgt agcgtttgcc tgtttggcaa ggacaagcgg tgattttttc taacattttt
     5161 acatttccgc ataaaaataa agatgtgaaa agtttacgct ataaataaag aaaaataaat
     5221 tttataaaaa agcgtgacat accccttgtg gataacttat gcaaaaatcg tgaatttttt
     5281 gggtattcat tgctttaaaa agcctttatt ctatagattg tggataactt aagattttat
     5341 ccacaggtta atttattggt taattgatag tattcaccta tttaaattat actaacttat
     5401 tgtttttaaa tgaaaagatc ttttgttcac agaaaaatta ggatctaata agagtaagaa
     5461 taaagatctt tatatagatc tatattatat ttagtgtcgc atttagatct taacctctct
     5521 aaaaatctat ttcccagatg atcccttttt acgctaaaat atgcgctcat ttttcagcag
     5581 aatttgtaag atagtagaag agatatgatt tacaaccaaa tttacgatgt gatcgtggtc
     5641 ggcggtggtc acgccggcac agaagcggcc ctagcaccgg cacgaatggg cttaaaaacc
     5701 ctgattttaa cccacaatgt tgatacctta gggcaaatgt cgtgcaaccc ggctatcgga
     5761 gggatcggca aaggccatct ggttaaagag atcgatgcga tgggcggttt aatggcaaaa
     5821 gcaacagacg ttgccgggat ccaattccgt accctaaaca actcaaaagg gcctgcggtg
     5881 cgtgccaccc gtgcccaagc tgaccgtgtg ctttaccgta atgcggtacg cactgcgtta
     5941 gaaaatcaac caaatttaga tattttccag caagaagtag tggatatttt ggtggaaaac
     6001 aaccgtgcag tgggtgcagt taccaaaatg ggcttaacgt ttaaagcccg tgcggtagtt
     6061 ttaaccgctg gtacgttctt agcgggtaag atccacattg ggttggataa ctatgcaggt
     6121 ggtcgtgccg gcgatcctgc tgcaacaatg ttggcagatc gtctgcgtga tctgaatttg
     6181 cgtgttgatc gccttaaaac aggtacacca cctcgcctcg atgcccgtac catcaacttt
     6241 gacgtacttg ccaaacagca cggcgatgag gtgttacctg tcttctcttt tatgggcgat
     6301 gtctcacagc acccacgcca aattccgtgc tacatcaccc acactaacga tcaaacccac
     6361 gatttaattc gcaacagtct tgatcgcagc ccgatgtaca ccggtattat cgaaggggtt
     6421 ggtccacgtt actgcccgtc tattgaagat aaagtaatgc gttttgccga tcgtaatagc
     6481 caccagatct accttgaacc ggaaggctta aatacgattg aggtttaccc gaacgggatc
     6541 tccaccagct tgccgtttga tgtgcaaatg gggatcgtca actcaatgaa aggcttggaa
     6601 aacacccgca tcatcaaacc gggctatgcg attgagtacg actacttcga cccgcgtgat
     6661 ttaaaaccaa cccttgaaac caaagcgatt gacggtttat tctttgccgg acaaattaac
     6721 ggcacgaccg gttacgaaga ggcaggtgcg caaggtttgc tggcagggat taacgtagcg
     6781 ttacaggttc aaggtaaaga ggcgtggtat ccaacccgtg atcaagccta caccggcgtg
     6841 ttggtggacg acctctgtac attaggcacc aaagagccat accgtgtgtt tacctctcgt
     6901 gccgaatacc gtctgttgct gcgtgaagac aatgcggaca ttcgtttaac accaaaagcc
     6961 cacgaactag gattaattga cgaggcacgt tgggtgcgtt tcaaccaaaa aatggaagcg
     7021 attgagcaag aaagagctcg tttgaaatcc acttgggcac atttgcaaat gccaaatctg
     7081 gacgaattga acgcgttatt ggctaatcct ttagcccgtg aagcaagcgg tgaagactta
     7141 atccgccgtc ctgaaatgag ttacgaaaag ctgacccaaa tcgccctatt tgcccctgct
     7201 attgaagaca agcaagcggc agaacaggtg gaaattgcga tcaaatatca gggctatatt
     7261 gagcatcaat acaacgaaat cgaacgccat aaacgccacg aaaacaccct gattccggta
     7321 gagtttgact acgacaaagt cgaaagcctc tccaacgaag tgcgagctaa attgatgcaa
     7381 caccgccctg tctccatcgg gcaggcaagt cgcatttccg gcatcacacc ggcagcgatt
     7441 tccattctgt tggtgaattt gaagaagcaa ggaatgttga aacggggtga gctgtaaata
     7501 ctacaagcgg ttggttttgc agaaattttt gcaaaattga ccgcttgtat tctttggaga
     7561 aggaaatacc cgaactaccg ccgtattttt aatgatatat ctgcgtaaac tcggtttttc
     7621 gccgttcgat caccaaatgt ttgcccaacc ttcgtggtat ttctgcaatg cacttgttca
     7681 taccaattat gaaaatctgc gacaaggtgt atttgtgact aaagcgtatt tagtgatgtt
     7741 tcttgaaaat ttattgtttg aaaaaagtca ttcgttaagt aatcgggcat tgcgtattaa
     7801 tgaaaataat gattaaggaa tttgcaaaat gttagctaaa ttaaaccgct tgcttgctca
     7861 agccgaaata aatttgaccg atcaacaaaa agagcagtta gttggttttg tgagattatt
     7921 ggataaatgg aataaggcgt ataacctcac ttccgttcgc aaccctgatg aaatgttggt
     7981 taaacatatt ttggacagcc ttgttgttag tcgtcattta caaggcgaaa cgtttattga
     8041 tgtcggcacg gggccggggc taccgggcat tccgctggcg attgctaacc ctgataaaca
     8101 gttcgtgcta ctagacagct taggcaaacg catcactttt atcaaaaatg cgttgcgtga
     8161 gcttggtatt aaaaacgtta ccccagtgca aagtcgagtg gaggaataca ctgaaagcca
     8221 gtttgacggc gtattaagcc gagcctttgc ctcgcttaat gatatgatcg actggtgtta
     8281 tcatttaccg gctgaaaacg gtaaatttta tgcattaaaa ggcgtgtatg atgagcagga
     8341 aataactgaa atcaaacaac cgattcagct agtggaagtg attaagcttg aagtacctga
     8401 gttggtagga gaacggcatt tagtagtgtt gaaaaagccc taatacattg gaagtttaat
     8461 gatacaagcg gtcgaatttg taaatttttt tgcaaatctg actgcttgtg ttaactattt
     8521 ttcataaagc atacctggtt aataaggtgt gcttttttta taacctatta tttcatctga
     8581 tttatttcta ttttgtgatg tgcttcacaa aaacaaaaaa acataatgta gtaaaaaata
     8641 ttcgtaattg aagcccttat ttctttacct gaaaataagc gaacttaatt atataacgag
     8701 gaggttcgta atgaacaggg taacaaataa ggcagatgag tttgctaaac tcagtacgtt
     8761 tgaacgtatt gcttatggta gtggcgactt agcccaaaat ttagtttttg gtacagttgg
     8821 tggtttctta ctcacttacg taactactgt aaacggcatt tctgccgtag caggagtaac
     8881 gattttctta attgtaaaat ggggaaatgt gttttgggat ccgtttgtcg gtgcctttat
     8941 tgacaaaagc aagccctcag cacagggtaa ataccgcccg tatttactga aatttgggat
     9001 tcctcttgtg gttttagctt cactactatt tcttcctctt gaatcagtac gtggctcagt
     9061 gatttatgct ttcctttctt attttgccac tgcgatgggt taatattcct tatggctcgc
     9121 tttctgcttc tcttactcga gacacagacg aaattgctaa attaaccaca acccgtatga
     9181 caatggcaaa tatcgctaat ttgtttgttt ataccttatt cccacttttt gtgtagttac
     9241 ttgcaccaca cgccgaatta aaaaacaccg gctttttcgg tattgagctc tatttaggtg
     9301 attatatgtc tgctgctgca ggccctgctt ggtttaaggt ttatgcggtc tatatggtaa
     9361 ttggatttgt ggcgttaatg ctctcttatt ttggtactaa agagcggatt attccaacag
     9421 cggaagaaac agataaagtc aagtattcag atttagttaa tgaactcaaa cgcaatcgtc
     9481 cattacagat tttagggtta ttcttcttaa taggttttac ttttatgttc ttcggaaata
     9541 cggtgtggcc tttttaccta aaatataata ttgggcattc tgagtggatt gcctccgtcg
     9601 gtttattagg ctctatcctt gggattttcg ttctagggca actttgtcta tgggtatggc
     9661 aatttgactc cttcaaatac tctccaacct taggctatat cggtcgtttc ttacaacaat
     9721 ggggattaac ttctgctacc ggttttatgt gggcgttagt gcctgaggtt gttacctttg
     9781 gagagtttaa gtcgaaaaaa cgtgtagcag gtattattaa tgcgttaatg ggcttgttct
     9841 ttaaaatcgg cttggcgtta ggcggtatta ttccgggtta tattaatgcg gtttatggtt
     9901 ttaacggctc gcttttggct caaaccgaag aggctttatt tggtatcaat ctttcaatga
     9961 tttgggctcc aattatttta gcgattattg caggatttgt gatgagtcgc tatccgttaa
    10021 gcgatactga tattgatcat atcaacgaaa aaattactgc ttattccaaa taatttgagt
    10081 ataggagaat agattatgca aattcaaaat ccagttttac ccggtttcaa tccagatcca
    10141 tctattgtgc gtgtgggtga tacttactac attgcaactt ctacttttga atggtttccg
    10201 ggagtgagaa ttcacgaatc gaaagattta gtgcattgga atttagttaa aaatgtctta
    10261 gatacaacca cgttgttaga tatgcgaggc aatccctctt caggtggtat ctgggctcct
    10321 gatctttctt atgcggaagg taaattttgg ttggtttata ccgatgtaaa aattacagac
    10381 ggtgcattca aagatatgac taactatcta actactgcca aagaaattca aggtccttgg
    10441 tcagctccga ttaaattgaa tggtattggg tttgatgctt cgttgtttca cgatgatgat
    10501 gggcgaaaat atttagtgca acagacttgg gatcaccgtg aatatcatca cccctttgac
    10561 ggtattacgt taacggaatt tgatgttgcc acaatgaaac tcaaacctga aacccagcgt
    10621 acgatttttg aaggtacaga agtgaaattg gtggaaggtc cacatattta taaaattaat
    10681 ggcgaatatt atctgtttgc ggcggaaggc ggcacagtat ttacgcatca agaagtagtg
    10741 gctcgttcta aaacattgga ggctcactct ttccaactgg ctccaaatac accgtttatt
    10801 actaattttg atacacctaa ttcttaccta caaaaacaag ggcacggttc attagttgat
    10861 accccaagtg gtgaatggta ttacgcttcc ctcacagcaa gaccttggaa tcatgcaaca
    10921 gaatccgcaa ccgatcctcg tggttggtca acattagggc gtgaagccgc cattcaaaaa
    10981 gtagaatttg atcaagacgg ctggccttat attgtgggtg gacacggtgg aaaaaccttt
    11041 gtagaagccc caaaagatgc aatttttacc gaagctccta ttgatcattc tcaacacgat
    11101 gagtttgata gctcaacgtt agatattaac tggaacactt aagagtgcct tttagtaaag
    11161 agatgggcga agttggtaat ggtgttttaa aattgattgg gcgaggctct cttgcaaata
    11221 cacacgattt atcgctaatc gcacgccgtt ggcaagcctt ctattttgat gcggaaacca
    11281 aagtgaaatt tacaccgttt tcttatcaat ctatggcagg tttaactaac tattacaatc
    11341 actcacactg gtcttggatt ttcatcacca aaaatgatca aggacaacag gtgattgaag
    11401 ttgcagaaaa taaaggtgga ctcagaaatg ggcaatatac ttcttacctt aaagataaag
    11461 caattgtcat tcctgatgga acggagtatg tttggtttaa gactaaagtt cggaaagaaa
    11521 cttaccgtta tgaatattca ttcgatggca aacaatggca cactattccg gttgaattag
    11581 atgcggcagt gctttcagat gattatgtgt tacgcagtta tggcggtttc ttcacaggag
    11641 cttttgtcgg gctggctgca gtggattact ccagttatga atctacagca gaatttagct
    11701 actttgacta taaagagtta ggcgataagc aacttacaga cggctcattc tcttgggaaa
    11761 aatctgaaag ttgctttggc taaaatgagg agaatatcac aatgaaacaa cgcagatttg
    11821 gtaaaaccaa caaaatggtg tcagaaattg ggttaggcac ttggcagctt gggacgaaat
    11881 ggggtgaacc gttcaatcat caagaggcga tggcaatttt agaaaccgct tatgagcaag
    11941 gcatcaattt tattgatacc gctgatgtgt ataacaacgg gcaaagcgag aaagccattg
    12001 gtgatatttt gaaaaaatac cctgactttt tctatgtcgt taccaaatgt ggacgtgcgt
    12061 taaacccgca tactgctgaa atgtacacgc cacaggcaat cgaaaaattt gttgatggta
    12121 gtttaagcca tcttggcacg gaaaagttgg atatgatttt gctccactgc ccgccaacgt
    12181 tggtttatcg caatgatgag atttttacga aattggaaca gcttaaagcc ggcggaaaat
    12241 taatagacta cggtgtgagt atcgaaacgg tagaggaagg cttgttggca atggagtatg
    12301 acattgcggc aatggaagtt atcttcaata tgttccgctt aaaaccattg gagcaactct
    12361 tcccaaccgc tcagcaaaaa aatgtgggta ttatcgcccg tgtactgttg gcaagtggtt
    12421 tgcttacagg cagatataat gcctgcaccc aatttggcaa agacgaccac cgcacattta
    12481 accgcaatgg tgaagccttc gataaaggcg aaaccttttc gggtgtggat tatcaactcg
    12541 gtttacaagc ggtggaagaa ttgaaagccc tattcggcac ggaagattta attccttacg
    12601 ccctacgttg ggtattgatg aatgaagctg tgagtacagt aatcccggga gcaagtaagg
    12661 tttcacaagt aattactaac gcagcggtgg ataatttccc tccactcact gaaaatgaaa
    12721 tgcaaaaagt ggaagatatt tacaactgct taattcgccc aagtgtgcat catttgtggt
    12781 aatttatcca acgctataaa aaatggaact ctaacttgag ttccattttt gtcaatttta
    12841 gccttaaacc tctaactgtt tagaggtttt cttaaacaga gtcaatgcaa taaacgcaaa
    12901 ggctaagaaa caggaaagga tcagggcata gaaaccaccg tcccagccga acatatcaac
    12961 cagtttaccc atcacataac ctgcacttgc agagcctaac aagtagccga ataaaccggt
    13021 taaacctgtt gctgtaccgg tggcaacacg aggcactaaa tccgctgctt gtaaaccgat
    13081 catcatcacc ggcccgtaga ttaagaaacc aatcgccatt aagcagatgt tgtcgattaa
    13141 cgggttgccg gctgggtttt tccagtacac cacaatcgca attaacacac cagcaaggaa
    13201 taacaacatc ggcggcgcac ggtgaccttt gaatactttg tcgcttaaat aaccgcttgc
    13261 aagcataccg aagataccgg catattcata caagaagtac gcccagctct gtttatccac
    13321 agagaaatgt ttcacttctt ttaagtaagt cggtgcccag tcgataatgc cgtagcgaat
    13381 gaaatagaca aacacgttgg caatcgcaat tgcccacagg aatttgttgt ttaaaatata
    13441 tttgtagaaa atgtctttgg aagagagtgt gtgggcagac tccacttttt ccaccacttt
    13501 ctcgcctttg tactcatcaa tcggcggtaa gccttgagat tccggcgtat cacgcatcaa
    13561 atagaacatc acaaacgcca gtacaatcgc aatcaacgcc ggcagataga acagggattg
    13621 ccacacgcca aaaatagata agcctaaaat cgccaacggc ccgattaaac cgccgcccaa
    13681 gttgtgcgac acgttccacc agctccacca cacgccacgt tcagaggttg agaaccagtt
    13741 ggtcatcgtt ttcgcccccg gcggatagcc cattccttgg aaccagccgt ttaacgccgc
    13801 caaaatgatc ataattggaa tagacgaaag cacgcccggt actaaaccga acaccagact
    13861 cacaatcgct gaaccgagca agccgatagt gataaagtat ttcgggttag aacggtcgga
    13921 aacgttaccc ataataaatt tactgaagcc gtaagcgagt gataatgcca cgcccaccgt
    13981 acctaagtct gctttggtga agccatattc atcaatcaaa tacggcattg ccaacgagaa
    14041 gtttttacga attaaataat atgccgcata gccgataaag acgcctgcaa acacttgcca
    14101 acgtaatttg cgatattccg catcaactct gctggcttcc actttttgtt ctgctggtgg
    14161 agaggctttt agaaaggaaa acattttact actcctttaa gaatgaaatc ctatggctac
    14221 tataatcttt catttggagc agaaaaagtg gatttttgaa aatttgtgag caagattaaa
    14281 attattttga gcgaaaacga aaatcggtta aatttccttc tttttttgca aaaaatttga
    14341 gaaatctaac cgcttgtttc acttaaaaat cagctatttc tgctttttca aaaattccac
    14401 gcccgtatca gggaagtcag taaagacgcc tgttgcaccc gctttattca gcagggcatc
    14461 gtacatttca tcgacatttt ggaaaaagtc cggcagagca tctttgcgca cggtgtatgg
    14521 gtgaagttcc attttgtact tcgccaactc tttcactaat ggcgtgtaaa caatgttgcc
    14581 gactttagat ttttctttat ccaccaacat ataccagccc gggccgacac catcggcata
    14641 ttttgccact tctgccatcg caccgtcttt gaacatccag tcgtagtcgt aattaaccca
    14701 gttgccgttt ttatctttct cttcggtttc gtgccaatcg gtgtaggcaa ctaactgcac
    14761 caattttacg tccataccca gtttcggtag taattcggtt ttgatccgtt tcagctcgtt
    14821 gaaatcgaag gtttgcaggt aaattggtga ggttttgctg tcgtagccat attttttcaa
    14881 cactttcagg gtttcgagtg caatgtcttt accctcttta tggtgcaacc aaggggcttt
    14941 aatttcagga tagatcccaa tttttttgcc ggttgatttc tctaatcctt gaataaattc
    15001 cagctcttct tccaaagtgt gaatggtgaa atgggactgc cacatcggga agcggttcgg
    15061 gtaaacttgt gcctgcttgc cgtctttcac ctcaaaattc tcggtcattt caagggtttt
    15121 aatctcatca agggtgaaat ccaccacata ataacgaccg tctgctcgtt tacgctcagg
    15181 gaattttttc gccacatcag ttaaaccgtc taaaaagtgg tcgtgaatca caatcaggcg
    15241 gttatctttg gtcatagcaa gatcttgctc taaatagtcc gcttgttgcc cgaatgctag
    15301 agctttagat tcaagcgtat gctcaggtaa ataaccgctt gcaccacggt gggcaatcac
    15361 taatttatca gattgttgtg cataggcacc caatgctgtc gcggacaatg ctaatgcagc
    15421 aaccaatgtt ttgaatttaa atttcataga attacctcat ataatgtaaa aataaaagga
    15481 agaacaaaaa tgtccttcct tttgataagt tatttattat ttaccatagt gatcttctaa
    15541 ttttgctttg tgcttgcctt cttcaaccat cacaatgaag gttaagagaa tggctaatac
    15601 accaccaccg atcatcacgt agaaaccgcc gtcccagcca aatttatctg ctgcccagcc
    15661 aactactgca gaggctgcca ctgtaccacc taagtaaccg aataaaccgg tgaaacctgc
    15721 tgctgtacct gctgcttttt tcggtgcaag ttcaagggcg tgtaagccaa tcaacattac
    15781 cgggccgtag attaagaaac cgatagtggt catccaaatg aagtccatta attgatatgg
    15841 gttttcatac caagcatggc ctgcatattg tgcaatttca gattccggtg tcgcagggtt
    15901 gagccagaac gcaaccactg ctgcggtggt aagaatcata aagatgaaac cggttaaacc
    15961 acgtttgcct ttgaacagtt tgtcagagac ccaaccacag agtaatgtac ctggaatcgc
    16021 agctaattca taaatggtat atgcccatgc tgtaccttta atgttgaagt gttttacttc
    16081 gcctaagtac accggcgacc atttcaatac gccatagcgg attaagtaaa cgaagacatt
    16141 cgcaatggca atgtaccata acagtttgtt tttcagcaca taagtcacga aaatctcttt
    16201 ggtgcttaaa tcgtgttcat aggttttttc gttatagtcg tcagggtagt cgttacgcca
    16261 tttttcaact ggcggtaaac cgcaagattg tggagtatct ttcatcacaa ggtagattgg
    16321 aatagctgcg atcattgctg caattcccgg gaaataaagg gcttgttgcc atacatcttt
    16381 tgccgttgct tctacaccat actcactgaa atagagcgca cttgctaata gcaccatcgc
    16441 ccccggcacc ataccgccta agttgtgagc ggtattccag atagaaacga tagtaccacg
    16501 ctcagattta gaccaccagt gaaccattgt tcgtccacac ggtggccaac ccataccttg
    16561 aaaccagccg tttaagaaga tcattaccca cattactaaa atgcttgatg ttgcccaagg
    16621 cattaagccc ataagggtca tacatagacc tgaaagtaac aatccggtcg gtaaaaatac
    16681 tttcgggtta gagcggtcgg acattcctgc catcacgaat tttgataaac cgtaggccag
    16741 gccggcagct gtaccaatca caccgagatc ggtttttgta tacataccag cttcaatcaa
    16801 gcctttttgt gctaagtcga agtttgcacg cacaaaatag taagcggcat agccgaagaa
    16861 aattcctgca aacacttgcc aacgcaagaa tttatatctt gcatcgactt tgtccgccgg
    16921 taattccgca atgtgcggag cgggtttaaa tggaccaaac atagtattct ccaatattac
    16981 ttagtaaaac ctctgagtaa ttccttttta ggaaattagg gaagcaataa taaatgaaaa
    17041 taaaaaatga caaagttatt ttttataaaa atgtgattca tttcacaaaa aatttacctt
    17101 aaaccacaaa aaat
//
LOCUS       DANJWC010000036        17063 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.036, whole genome shotgun sequence.
ACCESSION   DANJWC010000036 DANJWC010000000
VERSION     DANJWC010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17063
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.036"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..138)
                     /locus_tag="PWX02_000882"
     CDS             complement(<1..138)
                     /locus_tag="PWX02_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484215.1"
                     /note="response regulator in two-component regulatory
                     system with CpxA; part of the envelope stress response
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HDL1728912.1"
                     /translation="MSKILLIDDDIEFTELLTELLSLEGFKMKVVHNGLEGLNELESN
                     QY"
     gene            complement(206..988)
                     /gene="mazG"
                     /locus_tag="PWX02_000883"
     CDS             complement(206..988)
                     /gene="mazG"
                     /locus_tag="PWX02_000883"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HDL1728913.1"
                     /translation="MATIEQFLDVVNQLRHPEHGCPWDLKQNFDTMLPHLLEETYEVA
                     EAIHTQDRTALREELGDLLLQVVFLSQLAKEEGAFTFDDVVTDIHDKLIYRHPHVFGD
                     VKATNSEEALQSWEAQKANDAKHRSQDSILDDLPFALPALTRANKLQKRCAKVGFDWD
                     NPHDVLAKVEEELDEVKAEIAQYPARSAELAEELGDLLFAIVNLCRHYHTDAEEILRN
                     ANLKFEHRFRKVEKSVKKSGRTVKECKLEELDVIWHKIKVVE"
     gene            1298..2194
                     /gene="accD"
                     /locus_tag="PWX02_000884"
     CDS             1298..2194
                     /gene="accD"
                     /locus_tag="PWX02_000884"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HDL1728914.1"
                     /translation="MSWLDRILGRDSSSSSSAKSKVPEGVWTKCTSCEQVLYSEELQR
                     NQQVCPKCDHHMRIDARTRLLNLLDKDSAVEIAADLEPEDVLKFKDLKKYKDRLTAAQ
                     KQTGEKDSFIVLAGTLHNMPVVTASFNFEFMGGSMGSVVGAKFVKAAEKALAEKIPFI
                     CFSASGGARMQEALFSLMQMAKTSAILAKMKEQGVPFISVLTDPTLGGVSASLAMLGD
                     VNIAEPKALIGFAGPRVIEQTVREKLPEGFQRAEFLLEHGAVDMIVHRKEMRDILARL
                     CAKLTNQPTPFKVAELVVENAE"
     gene            2210..3511
                     /gene="folC"
                     /locus_tag="PWX02_000885"
     CDS             2210..3511
                     /gene="folC"
                     /locus_tag="PWX02_000885"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDL1728915.1"
                     /translation="MNTLSIPKATDSLETWLSYLEHAHSKPIDMGLDRIRKVATELDL
                     LKPAPYVITVAGTNGKGSTCRLLEVTLLKAGFRVGVYSSPHLIHYNERVRINGEVLPD
                     ADHVQAFAKINAKKTASLTYFEFSTLSAFELFKQNQLDIVILEVGLGGRLDATNIIDP
                     NLAVITSIDIDHVAFLGDNREDIGREKAGIFRENIPVVIGEPDCPHSIKQIADSLNCH
                     AFYRGKDWQFEINNNLFHWYSEEKSFTDLPIPLIPIPNAGTALAVLMQLPFEIDEKII
                     RQALNEAQMTGRFQTLTDTDFTHFSQPKPAAKVIIDVGHNPHAARYLADKLRELKQPN
                     QKIYAVFSALEDKDLSGIVEPLANIIDEWHCAGLDCWRGQTGEAVLAKLVNVLPNSTA
                     CSYENVAQASRVLFETANENDIVLVFGSFHTVADFMLWLET"
     gene            complement(3618..4808)
                     /locus_tag="PWX02_000886"
     CDS             complement(3618..4808)
                     /locus_tag="PWX02_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HDL1728916.1"
                     /translation="MFSHIQAAPADPILGLGEAFKADSREGKINLGIGVYMTDEGKTP
                     IVKAVKEAEKRLLETENSKNYLTIDGVQAFNAATQALLFGEGAEVITSGRAKTAQSLG
                     GTGALRIAAEFIKRHTNAKNVWISTPTWPNHNGIFDAVGVNVKGYRYYNKETNGLDWE
                     NLIADLSQAEAGDVVLLHGCCHNPTGIDPTPAQWEQLAALSAEKGWLPLFDFAYQGFA
                     NGLEEDAYGLRAFVKNNRELLVASSFSKNFGLYNERVGAFTLIADNADDANRAFTQIK
                     SIIRVLYSNPSAHGASALAVALADPDLKAQWIAELDEMRNRIKEMRSQLVQKLKEKGA
                     NKDFSFITEQNGMFSFSGLTPEQVDKLKDEFAIYAVRSGRINVAGITSKNIDALADAI
                     VKVL"
     gene            complement(4916..6889)
                     /locus_tag="PWX02_000887"
     CDS             complement(4916..6889)
                     /locus_tag="PWX02_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HDL1728917.1"
                     /translation="MRTIAIITSPNNADFIQQGYFYIPENHQLKAIPFSNAKTILGQE
                     YPFAIYTMQAENGVNFNLDAFAILAGTIQENGTLFLLCPQWDNLENELDFDALRWNEN
                     QAIACPNFYQHFKQLVAKFGFEVRTNLPKLPATSGQIPSKIYQLTQEQQNILQNLPLD
                     PADIHLITAPRGRGKSTLAGRLAKALPAQNKVLITARSYSALPSFWRSAEQKIPFFAP
                     DNLIEQVKSKNISPNQWLFIDEAASLPLPILHTLCAYFEKVVLTTTTQNYEGTGRGFE
                     LKFPVMLDKPFKHWTLSQPLRWSENDPLEAFVTELLLLSEEKSPLFSKEGKGEIYSLR
                     SHYVPLLKQRSNAKRLWQKQLSKNISYSNKHLQEKIAFYHLLANAHYKTTPTDLRRLF
                     DGENQLLFSQQENEQLIGGIWAMQEGGLDEKLTQAIWLGERRPQGSLVAQYLCFQGNL
                     PQACKLHSIRISRIAVLPEKQNQGVGKRLISQFILHNSVEKRPLDFISVSFGLTTPLH
                     AFWQSCGFQLVQITPNKEASSGYQSAMMIYPISEQGKLFAQQATQAFARDFALQPFFT
                     EICEELQNFEQFRPLATHNLDERDWKNLTAFAHGKRTFSAAYVSLKRLYLSDKARFSE
                     LDIFERKGQKISKMEVENYRKLVLQNLNNKSLK"
     gene            complement(6967..7449)
                     /gene="ybeY"
                     /locus_tag="PWX02_000888"
     CDS             complement(6967..7449)
                     /gene="ybeY"
                     /locus_tag="PWX02_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDL1728918.1"
                     /translation="MENLYIDLQIACENTENLPSEQQFYTWVQKALAVEAKTDDFPES
                     EITIRIVDEAESHELNLTYRGKDKPTNVLSFPFEAPEGIEMPLLGDLVICRQVMEKEA
                     EEQGISAESHWAHLAIHGTLHLLGYDHIEEDEAVEMESLETEIMQSLGYEDPYLSEKI
                     "
     gene            complement(7467..7922)
                     /gene="moaE"
                     /locus_tag="PWX02_000889"
     CDS             complement(7467..7922)
                     /gene="moaE"
                     /locus_tag="PWX02_000889"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HDL1728919.1"
                     /translation="MFETLIEVQEAEFDQNSIYQWLSEQHSVGAATLFIGKVREMNLG
                     DNVSGLFLEHYPAMTKKALQEIVAQARTRWDLQRVAVIHRIGQLHTGDEIVLVGVSSA
                     HRGDAYHANEFIMDYLKTKAPFWKREQTQAGERWIEGRESDQQAAKKWQ"
     gene            complement(7924..8169)
                     /gene="moaD"
                     /locus_tag="PWX02_000890"
     CDS             complement(7924..8169)
                     /gene="moaD"
                     /locus_tag="PWX02_000890"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HDL1728920.1"
                     /translation="MIKILFFAQVRELVATDRLDVEPTFTTAEALREHLSQQGKKWQL
                     ALEKGKLLVAINQTICPLETEIKDGDEVAFFPPVTGG"
     gene            complement(8162..8638)
                     /gene="moaC"
                     /locus_tag="PWX02_000891"
     CDS             complement(8162..8638)
                     /gene="moaC"
                     /locus_tag="PWX02_000891"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDL1728921.1"
                     /translation="MTTFTHINQNGEANMVDVSMKQETVRIARAEAFVRMNAETLQMI
                     VSGNHHKGDVFATARIAGIQAAKRTWELIPLCHPLLLSKVEVQLEALPESNQVRIESL
                     CKLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMIIENVRLLSKSGGKSGDFKVD"
     gene            complement(9139..10164)
                     /gene="moaA"
                     /locus_tag="PWX02_000892"
     CDS             complement(9139..10164)
                     /gene="moaA"
                     /locus_tag="PWX02_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDL1728922.1"
                     /translation="MQSIIPIKNVGDDVVSGLVDRFQRQYTYLRLSITDVCNFRCNYC
                     LPDGYKPPSHKQTFLSVDEIQRVAKAFANLGTEKIRITGGEPTLRKDFLEIAHTISQT
                     PGIRKVALTTNGYRLERDVELWQQAGITDINVSVDSLDPRQFQLITGENKLQSILKGI
                     DKAFEIGYPKIKVNAVLMKQYTAPELDKFLTWIKDKPIQMRFIELMETGEMDSFFQAQ
                     HLSGQSVMTRLLQEGWQLQPKAMSDGPAKVLSHPDFKGEIGLIMPYEKNFCASCNRLR
                     VSALGKLHLCLFGEEGIDIRDLLASDEQQLQLETRLKSSLQGKREHHYLHIGDSGVRN
                     NLASIGG"
     regulatory      complement(10153..10309)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            10473..12224
                     /locus_tag="PWX02_000893"
     CDS             10473..12224
                     /locus_tag="PWX02_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1728923.1"
                     /translation="MDKRSRWSCLVNEQGEISEWEDKAGADYFAELPQICHYAQALLY
                     SDKNSLQFYYSSNHQWLKISLERHQSFVLTQAEHTDLPYGLSKREIELLTLISSGLSN
                     EEIANELCISKRTVDKHIENIFAKTHTESRTLLAVFAITEHLVCLPTPGKLTQSILAS
                     FNIEQLALQIIAKKQPLVTPSTRHELPMFTISHHHTRPIIIGVPYVEQGIGQIDTREL
                     LNGTQLAVETINQQGGIHGREVQIATAGFCVKDKASILQAYHHLFDQEVDAISTAYAC
                     YLPEVHELIATKGIPYLHIATHSGSNKLAQNLPNQRIENMFQVCASDVTYGLGISHFL
                     QYYRYHYAPWLQNKTVMVVTVSWQKVDIGIESLTLSLREQGWNVDYLELTQEQDNYAK
                     LMTQVHNLTPSIIVFASYFAEDIVQFYQTFIRNPTNAIIYSIYAPSAFLPHEKLCEGV
                     LWSTNSGLSENYLGRQFSQHYEQFFGKSPTYSQASIAYDQANILANAWKQSVSPRHFK
                     AVSNAIRLQPHYGVNGTYYFNTDSQIGLTYAETHDLSISLPQLVYQIQQGQSRVIAPE
                     LFANAQFILPPWFSEKA"
     gene            12366..13481
                     /locus_tag="PWX02_000894"
     CDS             12366..13481
                     /locus_tag="PWX02_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDL1728924.1"
                     /translation="MAKMKKIAVFVGIMALGAAVLGGTQYAMKATSSTEFCVSCHSMT
                     HPQAEWEGSVHFSNRKGIRAECSDCHLPQDGLHYVKAKIIALKDVWHTVVTNKLPDQE
                     AYEAHRLEMAQRVWAEMKENDSMTCKSCHSFDAMVLSEQTEAAQKMHKLAQETNQTCI
                     DCHKGIAHFMPDVPVDNAAAMGELAKHGAEFSTNDKTLYTLAMTPVQLAQGGEIRLLP
                     YAELHQWTAEGEQVKATVNGWQQEGAESVVYMDLGKRIMVALLPDDAQSKVNVTKTVF
                     DNVTNSNWKEINFEITAPKNAVTANITALNHFGDNLNQTHCSGCHAPIGAEHYTANQW
                     IGVVNSMKDRTSMSADDVRAVTIYLQRNAKDVAGSSH"
     regulatory      13485..13680
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            13699..16185
                     /gene="torA"
                     /locus_tag="PWX02_000895"
     CDS             13699..16185
                     /gene="torA"
                     /locus_tag="PWX02_000895"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDL1728925.1"
                     /translation="MKKQDSIDVSRRGFLKNSSLSLAAGAGLASGTTGVVGALASTSA
                     KAAEMKTVVTAAHWGPLGVVVENGKVVKSDSVFEISVPNELQHVVADQVHGETRVKYP
                     MVRKGYLEGNKDTTIRGRDEWVRVSWDKAFELVANEMKRVREAHGANAIFGGSYGWYS
                     SGALHASRTLLQRYLNVTGGFVGVKGDYSTGAAQVIMPHVLGTIEVYEQQTSWEVILE
                     SSDIVVLWSANPLNTLRIAWTSTDQQGIEYFKKLKESGKRIICIDPVKSESCEFLGAE
                     WVPVNTGTDVALMLGMAHTLVAEGKHDQAFLKKYTTGFEKFEEYLLGKADNQPKDAKW
                     ASQICGVPEETIKKLATDFQSKRTMLMAGWGIQRQRHGEQGHWMLVTLAAMLGQIGLP
                     GGGFGLSYHYSNGGVPSATGGILGSITANPAVEAGAKTWLDETSKSSFPVARISDALL
                     NPGKTIDYNGTQITYPDIKIVYWAGGNPFTHHQDTNTLVKAWQKPDTIIVNECHWTPT
                     ARMSDIVLPATTSYERNDLTMSGDYSMMNIFPMKQVVEPQFEAKNDYDIFAELAKRAG
                     KEAEFTEGKSEMDWLQSFYQTAFEAARKNRVIMPKFEKFWQENKPLSFKASDKAKKWV
                     RYEEFRNDPLLNPLGTPSGKIEIFSEVIAKMNYDDCKGHPTWMEHEEYSGKTTAEEPL
                     ALVTPHSKWRLHSQLAYSSLRKQYAVNDREPVLIHTEDAAARGITSGDIVRIFNKRGQ
                     VLAGAVVTDGIIKGTLALHEGAWYDPLDLGTSEQPLCKNGNPNVLTRDEGTSKLAQGN
                     SPNTATVQVEKFTGQAPEVTVFKEPKYTQG"
     gene            16326..16487
                     /locus_tag="PWX02_000896"
     CDS             16326..16487
                     /locus_tag="PWX02_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase N-4/N-6"
                     /protein_id="HDL1728926.1"
                     /translation="MTTLTWYGKEEAVKKAKQTPYCLLRELPDFSHNIARQPTAQLLL
                     KPYLKSSKI"
     gene            16526..>17063
                     /locus_tag="PWX02_000897"
     CDS             16526..>17063
                     /locus_tag="PWX02_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL1728927.1"
                     /translation="MPFYAGQVKCIFIDPPYNTKSAFTHYDDNLEHSVWLSMMYPRLE
                     ILRDLLAEDGSIWITLDDNESHYLKIMLDEIFGRKNFISNLIWEKKRKPSFLGKVGKV
                     TDNLFCYAKNKNELNDLTYLETSGDETYPLYNAGNSREILVFPPRSIKFKKHSDGLYK
                     PTIFSKKTSSITLISELII"
BASE COUNT         5003 a         3508 c         3535 g         5017 t
ORIGIN      
        1 atattgatta gactctaact catttaagcc ttctaaacca ttatgcacaa ctttcatttt
       61 aaatccttct aatgaaagta attcagttag tagctcagta aactcaatat catcatctat
      121 taataagatt tttgacataa aatatttcct ataaaattaa aactaacttt acaaaggtaa
      181 aaattattat aacaattgat gttttttatt ctaccacttt tattttgtgc caaattacat
      241 ctaattcttc taatttacat tcttttacag ttcttcccga ttttttcaca gatttttcaa
      301 cttttcgaaa gcgatgttca aattttagat ttgcattgcg taaaatttcc tcggcatcgg
      361 tatgataatg gcggcataaa tttacaatcg caaacaagag atcacctaat tcttcagcaa
      421 gttcggcgga gcgagccgga tattgtgcga tttctgcttt gacttcatcg agttcttctt
      481 caacttttgc cagcacatcg tgcgggttat cccaatcgaa tccgactttc gcacaccgtt
      541 tttgtagctt atttgcacgg gtaagagcag gcaaagcaaa aggtaaatcg tctaaaatag
      601 aatcttggct acggtgtttt gcatcattcg ctttttgtgc ctcccaactt tgtaaggcct
      661 cttcgctatt agtagctttc acatcaccaa aaacgtgagg gtggcggtaa atcagtttat
      721 cgtgaatatc ggttactaca tcatcaaacg taaaagctcc ttcttctttc gctaattggc
      781 ttaaaaagac cacttgcagt aataaatcac ccaattcttc acgcagagca gtgcgatctt
      841 gggtgtgaat cgcttccgct acctcatagg tttcttccaa caagtggggc aacatcgtgt
      901 caaaattctg tttgagatcc cacgggcagc cgtgttcagg gtggcgaagt tggtttacaa
      961 catcaagaaa ttgttcaatg gttgccatag tatttccttt ttacaagcgg ttatttttga
     1021 tcaaattttt gcaaattcta ccatagcccg tactttttcg cttataatgc ggaaatttat
     1081 taagtatgag cagagcaatg gcagcggaaa aattaaccaa aaaacgctta attcaaattg
     1141 tggtgatgtt aatcattctt ctgattgcgt ttttttatcg aacttataca catagttagt
     1201 gatctgatat gtcaagaaaa catatcaaaa accgataaat tatgctttaa tagcaaccat
     1261 ttagtgaacc caacaactca atccaaaagg aataataatg agctggttag acagaatttt
     1321 aggtagagac tcttcgtcat caagcagtgc aaaatcaaaa gtgccggaag gcgtgtggac
     1381 aaaatgcaca agttgtgaac aagttctcta tagcgaagaa ttacaacgca accaacaggt
     1441 ttgtccaaaa tgcgatcatc atatgcgtat tgatgcccgc acccgtttat tgaatttgtt
     1501 agataaagat tctgcggtcg aaatcgctgc cgacttagag ccggaagatg tgttgaaatt
     1561 taaagattta aagaaatata aagatcgttt aaccgccgca caaaaacaaa ccggtgagaa
     1621 agattctttt atcgtgcttg ccggtacatt gcataatatg ccggtggtta cggcctcctt
     1681 taactttgag tttatgggcg gctcaatggg ttctgtggtg ggagcaaaat ttgtgaaagc
     1741 cgcggaaaaa gctttagctg aaaaaattcc gtttatctgt ttttcggcat cgggcggggc
     1801 tcgtatgcaa gaggcgttat tctctttaat gcaaatggca aaaaccagtg cgattttagc
     1861 caaaatgaaa gagcagggcg taccgtttat ttctgtatta accgacccaa ctttaggtgg
     1921 ggtttccgca agtttggcaa tgctgggcga tgtgaatatt gctgagccaa aagccctaat
     1981 cggctttgct ggtcctcgtg taattgagca aaccgtacgt gaaaaactgc cggaaggttt
     2041 ccaacgtgcc gagttcttat tagaacacgg ggcagttgat atgatcgtgc atcgtaaaga
     2101 gatgcgtgat atccttgccc gtctttgtgc aaaattaacc aatcagccaa cgccgtttaa
     2161 agtggcggaa ttggttgtag aaaacgcaga ataatttaac ttaatctgaa tgaatacatt
     2221 aagcatacct aaagccacgg attctttgga aacgtggctt tcctatttag aacacgccca
     2281 ttctaaaccg attgatatgg gcttagatcg tattcgtaag gtcgcaacag agctggattt
     2341 actcaagcct gccccttatg tgattaccgt tgccggtact aacggcaagg gttcgacctg
     2401 ccgtttgtta gaagttacct tattaaaagc cggatttcgg gttggggttt actcttcacc
     2461 gcacttaatt cactataatg aacgggtgcg aattaacggc gaagtattac ctgatgccga
     2521 ccacgtccaa gcatttgcaa aaattaatgc aaaaaagacc gcttcactca cttattttga
     2581 gtttagtacg ctgtctgcat ttgagctatt caaacaaaat cagctcgata ttgtgatttt
     2641 agaagtcgga ttaggtgggc gattagatgc cactaatatt attgatccaa accttgccgt
     2701 gattacctcg attgatatcg accacgttgc atttttaggc gataaccgtg aagacatcgg
     2761 gcgtgagaaa gccggcattt tccgtgaaaa tattccggtt gtcatcggcg agcctgattg
     2821 ccctcattcg attaaacaga ttgctgattc gttaaattgt cacgcttttt accggggtaa
     2881 agactggcag tttgaaatta ataataatct gtttcattgg tattctgaag aaaaatcctt
     2941 taccgattta ccaatcccgc ttattccgat tccaaacgcc ggcaccgcat tagcagtatt
     3001 aatgcaactg ccatttgaaa ttgatgaaaa gattattcga caagccttaa atgaagctca
     3061 aatgacaggg cgttttcaaa ccctgacgga taccgatttt actcattttt ctcaacctaa
     3121 accggctgca aaagtaatta ttgatgtagg gcataaccca cacgcagcac gttatttagc
     3181 tgacaaactg cgggagttaa agcaaccaaa tcaaaaaatc tatgcggtgt ttagtgcgtt
     3241 agaagataag gatttatccg gtattgtcga gccattagct aatattattg atgaatggca
     3301 ttgtgcaggg ctagattgtt ggcgtggaca aacaggcgag gcggtgttag cgaaattggt
     3361 aaacgttttg ccaaattcaa ccgcttgtag ctatgaaaat gtggcacagg caagccgagt
     3421 attgtttgaa acagccaacg aaaatgatat tgtgctggtg ttcggctcat ttcatacggt
     3481 agcagatttt atgctttggt tggaaaccta gttctacgaa taatcaacgt tggtgcaagt
     3541 gtccacactt gtacctaaaa gataaaaaag aggcacaagc actcaggctt atgcctctat
     3601 atatctttaa ggtttaatta aagcactttc acaatcgcat cggcaagagc atcaatgttt
     3661 ttgctggtaa tgcctgctac attgattctg cctgaacgca cagcataaat tgcaaactca
     3721 tcttttaact tatccacttg ctccggtgtt aagccactga atgagaacat accattttgc
     3781 tcagtaatga aagagaagtc tttgtttgcc cctttttctt ttaatttctg cactaattgg
     3841 ctacgcattt ctttgatacg gttacgcatt tcatctaatt ccgcaatcca ttgtgcttta
     3901 aggtcaggat cagccaatgc aactgctagt gcacttgcac cgtgagcaga tgggttagag
     3961 taaagtacac gaataatcga tttgatttga gtgaaagcac ggttggcatc atcagcatta
     4021 tctgcaatta gggtaaatgc accgacacgc tcgttatata acccaaagtt tttcgagaat
     4081 gagcttgcga ccaacaattc acggttattt ttcacaaaag ctcgtaaacc gtaagcatct
     4141 tcctctaaac cgtttgcaaa gccttgataa gcaaagtcaa ataacggcaa ccagcctttt
     4201 tcggcagata atgctgctaa ctgttcccat tgtgcaggtg ttgggtcaat tccggtcggg
     4261 ttgtggcagc aaccgtgcaa taacaccaca tctccggctt ccgcttggct taaatcggca
     4321 attaaatttt cccaatctaa accgttagtc tctttattgt agtagcgata gccttttaca
     4381 ttcacgccta ctgcatcaaa aatgccgtta tggtttggcc aagtcggggt tgaaatccat
     4441 acatttttcg cattcgtatg acgtttgata aattccgccg caatgcgtaa tgcccctgtc
     4501 ccacctaaac tctgtgcagt tttagcacga cctgaggtaa tcacttcagc cccttcgcca
     4561 aataataacg cttgcgttgc cgcattgaat gcttgaacac cgtcaatggt taaataattt
     4621 ttactgtttt ccgtctctaa taaacgtttt tctgcttctt ttaccgcttt gacaatcggg
     4681 gttttgcctt catcggtcat ataaacaccg ataccaaggt tgattttacc ttcacggcta
     4741 tctgctttaa acgcttcacc taagcctaaa attgggtctg ccggagcagc ttgaatgtga
     4801 ctaaacattg acattggagt tcctcatcaa aatgaaaaat aaacagtgaa tttatacgat
     4861 taaattgcag atttagcaag agcaaaaaca caaaaaatgt aataaaacct ataaattatt
     4921 ttaatgattt attgtttaag ttttgcaaaa cgagttttcg ataattttcc acctccattt
     4981 tggagatttt ttgccctttt cgttcaaaaa tatccagctc tgaaaagcgt gctttatccg
     5041 ataaataaag ccgttttaaa ctcacatacg ccgctgaaaa cgtccgcttg ccatgagcaa
     5101 acgcggttaa atttttccaa tcacgttcat ctaaattgtg tgtggcaagc ggtcgaaatt
     5161 gttcgaaatt ttgcaattcc tcacaaattt ccgtgaaaaa tggctgaagg gcgaaatctc
     5221 tggcaaaggc ttgggttgcc tgctgtgcaa ataactttcc ttgctccgaa atcgggtaaa
     5281 tcatcattgc actttgataa ccactgcttg cctctttatt cggtgtgatt tgcacaagtt
     5341 ggaaaccaca agattgccaa aatgcgtgga gtggagtagt tagcccaaag ctcacagaga
     5401 taaaatcaag cggtcgtttt tctaccgaat tgtgcaaaat aaactgagaa atcaaccgct
     5461 tgccaacgcc ttgattttgc ttttcaggca ataccgcaat ccgagaaatg cgaatggaat
     5521 gcaatttgca agcctgcggt aaattccctt gaaaacagag atattgagcc actagactgc
     5581 cttgcggacg gcgttcccct agccaaatgg cttgggttaa tttttcatct aaaccaccct
     5641 cttgcattgc ccaaattccg ccgataagtt gctcgttttc ctgttgtgaa aacaaaagct
     5701 gattttcgcc atcaaataaa cggcgtaagt cagttggagt ggttttgtaa tgggcgttgg
     5761 cgagtaagtg gtagaaagcg attttttcct gtaaatgctt attcgaataa cttatattct
     5821 tcgataattg cttctgccac aaacgctttg cgtttgaacg ttgctttagc aacggcacat
     5881 agtgtgagcg aagcgaataa atctccccct tcccctcttt gctaaagagg ggagattttt
     5941 cttcggaaag taatagcaac tcagtcacaa atgcttcgag aggatcattt tcgctccacc
     6001 gcaatggttg agataacgtc caatgtttaa acggtttatc caacattacc gggaatttca
     6061 gctcaaatcc tcgccccgtg ccttcgtaat tttgggttgt tgtggttaaa accacttttt
     6121 caaaataggc acaaagtgtg tggagtatcg gcaagggtag gctagctgcc tcatcaataa
     6181 agagccattg gttaggtgaa atgtttttac ttttaacttg ctcgattaaa ttgtcaggtg
     6241 cgaaaaaagg gattttttgc tctgccgaac gccaaaaact gggcaatgca gaataagacc
     6301 gtgcggtaat taatacctta ttttgtgcgg gtaacgcttt tgcaagtcgc cctgcaaggg
     6361 tagatttgcc ccgcccacga ggggcggtaa tcaaatgaat atcggcagga tcaagcggta
     6421 gattttgcaa aatattttgc tgttcttgcg ttaattggta aatttttgaa ggaatttgac
     6481 cgcttgtagc cggtaatttt ggcaaattag ttctgacttc aaaaccaaat ttagcgacta
     6541 attgtttaaa atgttgatag aaattcgggc aggcaatagc ctgattttcg ttccaacgta
     6601 acgcatcgaa atctaattca ttttccaaat tatcccactg aggacaaagc aaaaataaag
     6661 taccgttttc ttgaatagtg ccggcaagta ttgcaaaagc atctaagtta aaattcaccc
     6721 cattttctgc ttgcatagta taaatggcaa aagggtattc ttgtcctaaa atcgttttgg
     6781 cattggaaaa ggggatagct ttaagctgat gattttcggg aatgtaaaaa tagccttgtt
     6841 gaataaagtc ggcattgtta ggagatgtta tgattgcgat cgttctcata taacttgtag
     6901 gaacagctcc tgtgcctgtc cgattataat ggagtactgg agggcacagg accctcccct
     6961 acaagactat attttttctg aaagatacgg atcttcatac cctaaacttt gcataatttc
     7021 ggtttcgagg ctttccattt ccactgcttc atcttcctca atatggtcgt agccgagcag
     7081 atgaagtgtg ccgtgaatcg ccaaatgagc ccaatgggat tcggctgaaa taccttgttc
     7141 ttccgcctct ttttccatta cttgacgaca gatgactaaa tcacccaata gtggcatttc
     7201 gattccttcg ggggcctcaa acgggaaaga tagcacattg gttggcttgt ctttaccacg
     7261 ataagtcaga tttagctcgt ggctttctgc ttcatcgaca atccgaatgg tgatctcact
     7321 ctcagggaaa tcatcggttt ttgcctcaac tgcgagggct ttttgtaccc aagtataaaa
     7381 ttgttgttca cttggtaagt tttcggtatt ttcgcaggcg atctgcaaat caatatataa
     7441 attttccatt cgttaccttt tgcaaattat tgccattttt tggcggcttg ttggtcactc
     7501 tcacgccctt caatccaacg ttcaccggct tgggtttgtt ctcgtttcca aaatggggct
     7561 ttggttttga gataatccat aataaactcg ttggcgtggt aggcatcacc tcgatgagca
     7621 gaactcacac cgactaatac gatttcatca ccggtatgca gttgcccaat gcggtgaatc
     7681 accgcaacgc gttgtaaatc ccaacgagtg cgagcttgag ccacaatttc ttgcaatgct
     7741 tttttagtca ttgccggata gtgttcaaga aacaaaccgg aaacattatc gcccaaattc
     7801 atttcacgta ctttaccgat gaatagagtg gctgccccca cgctatgttg ttcactgagc
     7861 cattggtaaa tgctgttttg gtcaaattca gcttcttgta cttcaatcag ggtttcaaac
     7921 attttagccc ccggttaccg gtgggaagaa tgccacttca tcgccgtctt tgatctccgt
     7981 ttcaagcggg cagatcgttt gattaattgc aaccagcagt ttgccttttt caagggcaag
     8041 ttgccatttt ttaccctgtt ggcttaagtg ttcacgcaac gcttcggcag tggtaaaagt
     8101 aggctcaaca tctagtctat ccgttgccac aagttcacgc acttgggcga aaaaaagtat
     8161 cttaatcaac cttgaaatct cctgattttc cgccgctttt actaagtaat cttacatttt
     8221 caataatcat atctttttgc acggctttac acatatcgta aatagtcaga gcagcaacac
     8281 tagctgcggt taatgcctcc atttccacgc cggttttgcc ggttagttta cagagtgatt
     8341 caattcgcac ttggttgctt tccggcaacg cctccagttg aacttccact ttagaaagca
     8401 ataacgggtg gcagagtgga atcagctccc aagttctctt cgccgcctga ataccggcaa
     8461 ttcgagcggt tgcgaataca tcgcctttgt ggtgattacc actgacgatc atttgtaatg
     8521 tttcggcatt cattcgcaca aatgcctccg ctcgggcaat gcgtacggtt tcttgtttca
     8581 ttgaaacatc aaccatattg gcttcgccgt tttggttgat gtgggtgaag gttgtcattt
     8641 gtttgtctct ttaaatttga ttgtaaaatt aatattgagc gttttcttgt tactttcttt
     8701 gcttgtgcaa agaaagtaac caaagaaagc acaccctact tcaccgctat cttcatttta
     8761 tttgaatttg tttaacggaa aaatttaaaa ctcgccaact tcgttggctc aaacactaaa
     8821 tttttcctat aaattcaaag tcgctcaggc ggttcagaag ggagaagctg gaacattaca
     8881 agcggttatt tttaaataaa aacttgcaaa aatcttaaat aatgtaccgc ttgcccctat
     8941 tttccgccgt agcgattgcg taaacttttg aggacaactt ttcgtgtttg agccaacgca
     9001 gttggcgagt ttgaaaagtt cgtcccaaaa gttagcaaca agcgaaggat taaggaaaat
     9061 ctggggtcgc ctttcttttg gttacttttc tttggcgaag caaagaaaag taacagaaaa
     9121 aacacgctcg gtggttgttt aaccaccgat actcgccaaa ttattcctga ccccactatc
     9181 cccaatatgc aaatagtggt gttctcgctt gccttgtaag gaagatttta ggcgggtttc
     9241 aagctgtaac tgttgctcat cgcttgccag taaatcacga atatcaatcc cttcttcacc
     9301 aaataagcaa agatgcagtt taccaagtgc ggaaacacgc aagcggttgc aagaggcaca
     9361 gaaatttttc tcgtagggca taattaagcc gatttcgcct ttaaaatcag ggtgagaaag
     9421 cacttttgcc gggccgtcag acatcgcttt gggttgtaat tgccagcctt cttgtaataa
     9481 gcgggtcatc accgattgac cagaaagatg ttgagcttgg aagaaactat ccatttcacc
     9541 ggtttccatc agctcaataa accgcatttg aatcggcttg tctttgatcc aagtgaggaa
     9601 tttatccaat tcaggggcgg tatattgctt cattagcacc gcattgactt taatttttgg
     9661 gtagccaatt tcaaaggctt tatcaatacc ctttaaaata gattgcaact tgttctcgcc
     9721 ggtaatcagt tgaaattggc gtgggtcaag gctatcaaca ctcacattaa tatcggtaat
     9781 accggcttgt tgccacagtt caacatcacg ctctaggcga tagccgttgg tggttagggc
     9841 aactttacgg ataccgggtg tttgtgaaat agtgtgagcg atttcgagga aatctttgcg
     9901 taaagttggc tcaccaccgg tgatacggat tttttccgtc cctaaattgg caaaggcttt
     9961 agccacccgc tggatttcat caacgctaag aaaagtttgc ttatgtgaag gcggcttata
    10021 gccatcaggc aagcaataat tacaacggaa gttacaaaca tcagtaatgg atagtcgcaa
    10081 ataagtatat tgacgctgaa agcgatcaac taaacctgaa acgacatcat cacccacgtt
    10141 tttaattggg ataatagact gcatttgaca cctttctaaa cacgagagga caacccattt
    10201 ctaagttatc ctttgaacag ctttatgctg ttactggcat aaattccata tcaaaaattt
    10261 gtctttaatt tttagagtta ggaaataatg ctcggagtct tcaataaatt ggttttcatt
    10321 ttatggtgag aaacctaatt aggcaagagg tataatttaa attgtatgct ctacctataa
    10381 aaatctggca accgttgctt agtttgattt atatcatatt aatgccgaga taattttgct
    10441 attttagcta acgtttttag ttcgaggtga gaatggataa acgtagccga tggtcttgtt
    10501 tagtaaatga acaaggcgaa atatcagaat gggaagacaa agcaggggca gactattttg
    10561 ctgaattacc gcaaatttgc cactatgcac aggcattgct ctattcagat aaaaatagct
    10621 tgcagtttta ctatagttca aaccatcaat ggcttaagat cagcctagaa cgccaccaat
    10681 cgtttgtatt gactcaagca gagcataccg atttgcctta tggactgagt aaaagggaga
    10741 ttgagcttct cactctaatt agcagtggtt tgagcaatga ggaaatcgcc aatgagcttt
    10801 gtatcagcaa acggacagtt gataaacata tcgaaaatat ttttgcaaaa acacacaccg
    10861 aaagccgaac cttattagcg gtttttgcta ttaccgaaca tttagtctgt ttgcctacac
    10921 cgggaaagct aacccaatcc attcttgcca gctttaacat tgaacaactg gcattgcaaa
    10981 ttatcgcgaa aaaacaaccg cttgtaacgc cttcaactcg gcacgaacta ccgatgttta
    11041 ccataagcca ccatcatact cgcccgatca ttattggcgt gccttatgtt gaacagggta
    11101 ttgggcaaat tgatacccga gagttactta acggcactca acttgcggtt gaaaccatca
    11161 atcaacaagg ggggattcac ggtagagaag tgcagattgc aacggcaggg ttttgcgtta
    11221 aagataaagc aagtattctt caggcatacc atcatttatt tgatcaagaa gttgatgcca
    11281 tttcaaccgc ttatgcgtgc tacttgccgg aagtacacga attaattgca actaaaggca
    11341 ttccttattt gcatattgca actcatagtg gctcaaataa acttgcccaa aatttaccga
    11401 atcaacgaat tgaaaatatg tttcaggttt gcgccagtga tgtaacctat ggcttaggta
    11461 tatctcactt tctgcaatat taccgttacc attatgcccc ttggcttcag aataaaacgg
    11521 tgatggtggt aacggtgagt tggcaaaaag ttgatatcgg tattgaaagt ttaaccctaa
    11581 gtttgcggga gcaaggctgg aatgtggatt atctggaact cacccaagag caagataatt
    11641 atgccaaatt aatgacacag gtgcataatc tcacgccatc cattattgtg tttgcctctt
    11701 attttgcaga agatattgta cagttttatc aaacgttcat tcgcaaccca acaaatgcca
    11761 ttatttacag tatttatgca ccttcggcgt ttttgccaca cgagaaactt tgcgagggcg
    11821 ttctctggag taccaatagt gggttaagcg aaaattattt aggcagacag tttagccaac
    11881 attacgaaca atttttcggc aaatcgccca cttattccca agccagtatt gcttatgacc
    11941 aagcaaatat tctagcaaat gcgtggaaac aaagtgtttc acctcggcat tttaaagccg
    12001 tctctaatgc gatccgctta cagccacatt atggggtaaa cggcacttac tattttaata
    12061 ccgatagcca aattggatta acttacgcag aaacccacga cttatctatc agcttgccac
    12121 agttggttta ccaaatccaa caagggcaga gccgtgtgat tgcaccggag ttgtttgcca
    12181 atgcacaatt tatcttaccg ccgtggttct ctgaaaaagc ctaaaatttc actacatatt
    12241 tttgccttag atcaaattat tctcactctt tcgaggtaat aggtattttt acttattttt
    12301 atacctcttg ggagttaata caatgcctct gtctttaatt tttagtgtat taagtggggt
    12361 aatttatggc taagatgaaa aaaattgctg tatttgtcgg tattatggca ttaggtgctg
    12421 cggtattagg tggcacacaa tatgcaatga aagccaccag ctcaacagaa ttttgtgtga
    12481 gctgccattc tatgactcac ccacaggcag agtgggaagg cagtgtccat ttttccaacc
    12541 gtaagggaat tcgagccgag tgttcggatt gccatctacc acaagacggc ttgcattatg
    12601 tcaaagctaa aatcatcgca ttaaaagatg tttggcacac cgttgtaacc aataaattac
    12661 ctgatcaaga agcctatgaa gcccatcgtc tagaaatggc acaacgtgtt tgggcagaaa
    12721 tgaaagaaaa cgattctatg acctgtaaaa gctgtcatag ttttgatgcg atggtacttt
    12781 ctgaacaaac agaagcggca cagaaaatgc acaaactagc ccaagaaact aatcaaacct
    12841 gtattgattg ccataaagga attgcccatt ttatgcctga tgtgccggta gataatgccg
    12901 cagcaatggg cgaattagct aaacacggtg cggaatttag cacaaatgat aaaacccttt
    12961 atacgctcgc aatgactcca gtgcagctcg ctcaaggtgg agaaattcgc ttgttacctt
    13021 atgcagaatt acatcaatgg acagcagaag gcgagcaggt taaagcaacg gttaatggct
    13081 ggcaacaaga aggtgcggaa tctgtggtgt atatggattt aggcaagcgt attatggttg
    13141 ctcttctacc tgatgacgct caaagcaaag tgaatgtcac aaaaacagtg tttgataatg
    13201 tcaccaactc aaattggaaa gaaatcaatt ttgaaattac tgctcctaaa aatgcagtca
    13261 ctgccaatat tactgcacta aatcattttg gcgataactt aaaccaaaca cactgtagcg
    13321 gttgccatgc accgattggg gcagaacatt acaccgcaaa tcaatggatt ggggtggtaa
    13381 attcaatgaa agacagaaca tctatgtcgg ctgatgatgt gcgtgcggtg actatctatc
    13441 tccaacgtaa tgcaaaagat gtagcaggca gttcccatta attgtaactt agaggatacc
    13501 gagcttgtga gcctaagtgt ctactgatag cagagatatg gtagcaagcc gtaaagcacc
    13561 tattacaagc ggtcattttt tcctaacatt ttgcaaaaat taccgtcggt tctttaccaa
    13621 tggatacacg agaaatggtt tatccactcg actttagaaa ggtgtctatt tttaacttaa
    13681 cttcaaacga ggtttattat gaaaaaacaa gacagtattg acgtaagtcg tcgcggtttt
    13741 ctaaaaaatt catcattaag cttagctgcc ggtgcaggtt tagccagtgg tactaccggt
    13801 gtagtggggg cattagcttc caccagtgcc aaagctgccg agatgaaaac agtagtaact
    13861 gctgcccact gggggccttt aggcgtagtg gtagaaaatg gcaaagtggt gaaatctgat
    13921 tctgtttttg agatttctgt gccaaatgaa ttgcaacacg tggtagcaga ccaagtacac
    13981 ggtgaaacac gagtaaaata cccaatggtg cgtaaaggct acttagaagg taataaagat
    14041 actactatac gtgggcgtga tgaatgggtg cgagtttcgt gggataaggc atttgaatta
    14101 gttgcgaatg aaatgaaacg tgttcgtgag gctcacgggg cgaatgctat ttttggtggt
    14161 tcttacggtt ggtatagttc tggggcatta cacgcttccc gtacactgtt acaacgctat
    14221 ttaaatgtta ccggcggatt tgttggcgta aaaggcgact attctaccgg tgcagctcaa
    14281 gtaattatgc cacacgtttt aggtacaatc gaggtgtacg aacagcaaac cagttgggaa
    14341 gtgattttag aaagttcaga tattgtggtg ctttggtcag ctaacccgtt aaatacgcta
    14401 cgcattgcgt ggacttcaac cgatcaacaa ggtattgaat actttaaaaa actcaaagag
    14461 agcgggaaac gcattatctg tattgacccg gtaaaaagcg aatcttgtga atttttaggt
    14521 gcagaatggg taccggtaaa taccggcacg gatgttgcgt taatgctcgg tatggctcat
    14581 accttagtcg ctgaaggcaa acacgatcaa gcattcctta aaaaatacac caccggtttt
    14641 gagaagtttg aagaatatct gctcggtaag gcagacaatc aaccgaaaga tgcaaaatgg
    14701 gcaagtcaaa tttgtggtgt gccagaagaa accattaaaa aattagccac tgatttccaa
    14761 tctaaacgca caatgttaat ggcaggttgg gggattcaac gccaacgaca cggtgaacaa
    14821 gggcattgga tgttagtcac cctagctgca atgttaggtc aaatcggctt accgggtggt
    14881 ggtttcggtt taagctacca ttactcaaac ggcggcgtgc cgagtgccac agggggcatt
    14941 ttaggctcta tcacagcaaa tccggcagta gaagccggag cgaaaacctg gttagatgaa
    15001 acatcaaaat cttcattccc ggtggctcgt atttccgatg cactgctcaa cccgggcaaa
    15061 accattgatt acaatggcac acaaattact tatcctgata tcaaaattgt ctattgggca
    15121 gggggtaatc cgtttaccca ccaccaagac accaacactt tggtgaaagc gtggcaaaaa
    15181 ccggatacta ttatcgtcaa tgaatgccat tggacaccaa cggctcgtat gtctgacatc
    15241 gtgctgccgg caaccaccag ctatgagcgt aatgacttaa ctatgtcggg cgattattca
    15301 atgatgaata ttttcccgat gaaacaggtg gtcgaaccgc aatttgaggc aaaaaacgac
    15361 tacgatattt ttgccgaact agccaaacgt gcaggcaaag aagcggaatt taccgaaggc
    15421 aaatctgaaa tggattggct acaaagtttc tatcaaactg catttgaggc agcacgcaaa
    15481 aatcgtgtga ttatgccgaa attcgagaaa ttctggcaag agaataaacc actttctttt
    15541 aaagcatcag acaaagcgaa aaaatgggtt cgttatgaag aatttcgtaa tgacccactc
    15601 ctaaatccat taggcacacc gtccggcaaa attgagattt tctctgaggt gattgccaaa
    15661 atgaattatg atgactgtaa aggtcaccca acttggatgg aacacgaaga atactcgggt
    15721 aaaaccaccg cagaagaacc gttggcattg gtgactccac actctaaatg gcgtttacac
    15781 agccagttag cttactcatc attacgcaaa caatatgcgg ttaatgaccg tgagccggtg
    15841 ttgatccata ctgaagatgc cgccgctcgt ggcattacaa gcggtgatat tgtgcgtatt
    15901 tttaacaaac gtggtcaagt attagccggt gcagtggtga cagatggcat tatcaaaggt
    15961 acattagcct tgcacgaagg agcttggtac gatccgctcg atctcggcac cagcgagcaa
    16021 ccgctatgta aaaacggcaa ccctaacgtg ctaacccgtg atgaaggcac atcaaaatta
    16081 gcacagggta actcaccaaa taccgctacg gtacaggttg aaaaattcac cggacaagca
    16141 ccagaagtaa cagtatttaa agagcctaaa tacacacaag gctaatttca tataaccaaa
    16201 ggcacagtat aaaactgtgc cttttctatg ttgcaagcgg ttaaatttgc ttaatttttt
    16261 gtaaaacttt ctattttttt gaccgcttgt tacaataatc cacttttact tttcaggtgc
    16321 attttatgac aacccttact tggtacggca aagaagaagc cgtcaaaaaa gcaaaacaaa
    16381 ccccatattg cctattgcgt gagttgccgg acttctcgca taatattgcc cgacagccga
    16441 cagctcagct tctgctgaaa ccttatctaa agtcaagcaa aatttaatta ttcagggtga
    16501 taatttatca gccttaaaat ctcttttgcc cttttatgcg gggcaagtca aatgtatttt
    16561 tattgatcct ccctataaca caaaatcggc ctttacccac tacgatgata atttagagca
    16621 ttccgtttgg ctttcaatga tgtatccacg ccttgaaatt ctgcgagatt tacttgccga
    16681 agatggttca atttggatca ctcttgatga taacgaaagt cactatttga aaattatgct
    16741 tgatgaaatt tttgggcgga agaatttcat ttctaactta atttgggaaa agaaaaggaa
    16801 accatctttt ttaggcaaag ttggtaaagt tacagataat ttattttgct atgcaaaaaa
    16861 caaaaatgaa ttaaatgact taacttacct tgagacctca ggtgatgaaa cctatccatt
    16921 atacaatgct ggaaattcaa gagaaatttt agttttccct cctcgttcta taaaatttaa
    16981 aaaacatagt gatggattat ataagccaac cattttttct aaaaaaacga gttcaattac
    17041 gttgatttcg gaattaatta ttg
//
LOCUS       DANJWC010000037        16916 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.037, whole genome shotgun sequence.
ACCESSION   DANJWC010000037 DANJWC010000000
VERSION     DANJWC010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16916
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.037"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            60..2924
                     /locus_tag="PWX02_000898"
     CDS             60..2924
                     /locus_tag="PWX02_000898"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012771641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDL1728928.1"
                     /translation="MTKQFTMEDRFNPSAVEQALYQHWESQGYFKPSEDINAPSYCIA
                     IPPPNVTGSLHMGHAFQQTLMDTLIRFNRMEGNNTLWQTGTDHAGIATQMVVERKIAA
                     EEGKTRHDYGREAFINKIWDWKAYSGGTISQQMRRLGNSIDWDRERFTMDEGLSNAVK
                     EVFVRLHEEGLIYRGKRLVNWDPKLHTAISDLEVENKESKGSLWHFRYPLANGAKTAD
                     GLDYLVVATTRPETMLGDTAVAVHPEDERYQALIGKSVILPLANREIPIIADEYVDRE
                     FGTGVVKITPAHDFNDYEVGKRHGLPMVNVMTLNADIRAEAEIIGTDGKPLSDYTAPI
                     PADYQSLERFAARKKIVADFEALGLLDQIKPHDLKVPYGDRGGVPIEPMLTDQWYVSV
                     KPLAEVATKAVEDGEIQFVPKQYENLYFSWMRDIQDWCISRQLWWGHRIPAWYDEQGN
                     VYVARDEAEVRAKHNLPADLPLKQDEDVLDTWFSSGLWTFSTLGWPEQTKELKMFHPT
                     DVLITGFDIIFFWVARMIMFTMHFIKDENGKPQVPFKTVYVTGLIRDEQGQKMSKSKG
                     NVLDPIDMIDGISLDDLLEKRTGNMMQPQLAEKIAKATRKEFAEGIVAHGTDALRFTL
                     TALASNGRDINWDMKRLEGYRNFCNKLWNASRYVLTNDKLDLSEGDVEFSLADRWIES
                     QFNRTVETFRTALSQYRFDLVANAIYEFTWDQFCDWYLELTKPIFFKGTDVQRRGASR
                     TLVNVLEKLLRLIHPVMPFITEEIWQKVKGFVGIEADTIMLQKFPQFDPLAIDETAES
                     QINFIKEVIVAVRNIRAESNIAPSKGLDLIARNFSADEVSILNANEVLLKSMAKLDSV
                     KVLENGENAPLSVAKLVANGEILIPMAGFINKEAELARLTKEMDKLKGEVARIEGKLS
                     NEAFVAKAPEQVIAKEREKMQEYLSGLEKLQVQYQEIEAL"
     gene            complement(2995..3588)
                     /locus_tag="PWX02_000899"
     CDS             complement(2995..3588)
                     /locus_tag="PWX02_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HDL1728929.1"
                     /translation="MLTDQDIFNRYQTKKSEFEKQADISLIGHSLFDMWGDMENGTLN
                     LAGKTVANLGLSGTSTRQYLDVITRQERIQHVGDNVFIFLGVNDIVKEPDYSPKQVLE
                     WIGEIVDSLRKINPNANFFLLEATPVNNITTTNNAAIKELNAYFEANIPSDLTYVKTW
                     EYFADKEGNLDLTLCHDGLHFTQAGYDRLEAILTKHL"
     gene            complement(3723..5177)
                     /gene="gnd"
                     /locus_tag="PWX02_000900"
     CDS             complement(3723..5177)
                     /gene="gnd"
                     /locus_tag="PWX02_000900"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005714317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HDL1728930.1"
                     /translation="MSQKGDIGVIGLAVMGQNLILNMNDNGFKVVAYNRTTSKVDEFL
                     EGAAKGTNIIGAYSLEDLAAKLEKPRKVMLMVRAGEVVDQFIEALLPHLEEGDIIIDG
                     GNSNYPDTNRRVKALAEKGIRFIGSGVSGGEEGARHGPSIMPGGDESAWQYVKPIFQA
                     ISAKTDKGEPCCDWVGRDGAGHFVKMVHNGIEYGDMQLICEAYQFLKDGLGLSYDEMH
                     EIFKEWKNTELDSYLVDITTDILAYKDEDGEPLVEKILDTAGQKGTGKWTGINALDFG
                     IPLTLITESVFARCVSAFKDQRVAASRLFNKEIGKVEGDKKVWVEAVRRALLASKIIS
                     YAQGFMLIREASEQFGWDINYGNTALLWREGCIIRSRFLGNIRDAYEANPYLVFLGSD
                     PYFKDILENCLADWRKVVAKSVEIGLPVPCMASAITFLDGYTSARLPANLLQAQRDYF
                     GAHTYERTDKARGEFFHTNWTGRGGNTASTTYDV"
     gene            complement(5260..5949)
                     /locus_tag="PWX02_000901"
     CDS             complement(5260..5949)
                     /locus_tag="PWX02_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HDL1728931.1"
                     /translation="MKTLGILGGMSAESTVSYYLDINRSVNKVLGGNSSAKILISSVN
                     FEEIVQCQKQGNWQAAAEILSEQARLLEQAGAEGILLATNTMHKIADQISSHISVPFL
                     HILDCVAEAISAQGLQKVALLGTKFTMSDNFYKDGLLERGITSIVPDEETQQEIHRII
                     FEELCLGKITESAKAFYLQTIQQLSEQGAEGVILGCTEIGLLINQNDNPLPFFDTAAL
                     HSQMAVEFILG"
     gene            complement(5959..6846)
                     /locus_tag="PWX02_000902"
                     /pseudo
     CDS             complement(5959..6846)
                     /locus_tag="PWX02_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249554.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            complement(7022..7720)
                     /gene="pgl"
                     /locus_tag="PWX02_000903"
     CDS             complement(7022..7720)
                     /gene="pgl"
                     /locus_tag="PWX02_000903"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HDL1728932.1"
                     /translation="MNKVIFPTAQAAVEQIAKEFIEYSHQNRPVHISLSGGSTPKLLF
                     KTLAQAPFNTEIQWQNLHFWWGDDRMVAPTDPESNYGEVQKLLFDHIDIPSENIHRIR
                     GEENVEQELARFSAELTACVPNLAFDWIILGMGTDGHTASLFPHQTDFNDQNVAVIAK
                     HPETGQIRISKTASLIEKAKRITYLVTGASKAEILKEIQTTPAENLPYPAAKIQAENG
                     VTEWYLDSEAANLL"
     gene            complement(7770..9257)
                     /locus_tag="PWX02_000904"
     CDS             complement(7770..9257)
                     /locus_tag="PWX02_000904"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDL1728933.1"
                     /translation="MNAENSCIVIFGASGDLTFRKLIPALYNLYKIGRLGEHFSVLGV
                     SRSELTDESFRQKMRDALVKFEKASGEELDKFCEHLYYQAVNTSDAVDYAKLLPRLDE
                     LHDKYQTAGNTLYYLSTPPSLYGVIPECLAAHGLTTEEFGWKRIIVEKPFGYDIETAK
                     KLDVQIHKCFEEHQIYRIDHYLGKETVQNLLVLRFSNGLFEPLWNRNFIDYVEITGAE
                     SIGVEDRGGYYDGSGAMRDMFQNHLLQVLAMVAMEPPAIINANSMRDEVAKVLYCLHP
                     LTEEDVKHNVVLGQYARGTVDGKEVPAYLEEKGVPADSNTETFMAVKCEIDNWRWAGV
                     PFYVRTGKRLPTRVTEIVIHFKTTPHPVFSQNAPENKLIIRVQPDEGISMRFGLKKPG
                     AGFEAKEVSMDFRYSDLSSSSSLLTAYERLLLDALKGDATLFARTDAVHACWKFVQPI
                     LGYKANRGRVYEYESGTWGPTEADKLIARHGKVWRKPSGTMKKKV"
     gene            complement(9332..10150)
                     /gene="cysQ"
                     /locus_tag="PWX02_000905"
     CDS             complement(9332..10150)
                     /gene="cysQ"
                     /locus_tag="PWX02_000905"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HDL1728934.1"
                     /translation="MQTLTQPLLENIINIAKAAGEQLKQLYARSVAIKIKADCTPVTE
                     ADLIISQFLTERLKQLTPDIPVLSEEFCDIPFAERQQWQEYWIIDPIDGTQQFINRTG
                     QFSVVIALVQKREGRNQPVLGVIHAPILGKTYFSMEGSGSFLQENGEIRPLVKPKRAE
                     NALLIVTVGSDLHAEQAKNAISAPQIECVKYGSGSLKAGLVAEGKADCYIRFGDTGEW
                     DTAVAEVLLSEMGGKIFDFNFSPLTYNQRESLINPYFVMVADGSIDWDKIFLFK"
     gene            complement(10164..10706)
                     /gene="nudE"
                     /locus_tag="PWX02_000906"
     CDS             complement(10164..10706)
                     /gene="nudE"
                     /locus_tag="PWX02_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HDL1728935.1"
                     /translation="MIKQLPEILNIKTVAKSRIFEVQAVDLRFSNGEERTYERLTPNR
                     RSSVMVIPIQNNELIFVKEYAVGSERYELGFPKGIVDAGEEPIESANRELQEEIGFGA
                     KKLDFLRSLYSSPSHMYGLMHLFLAQDLYPSKLEGDEPEPLEIVRYPLDKIDQLLADP
                     DFAEARNLSALFMLREYLKK"
     gene            10794..11210
                     /gene="hslR"
                     /locus_tag="PWX02_000907"
     CDS             10794..11210
                     /gene="hslR"
                     /locus_tag="PWX02_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HDL1728936.1"
                     /translation="MKNKHEKEQNDAEVRLDKWLWAARFYKTRSIAKAMIEGGKVHYN
                     GQRAKVSKAVEIGAIIKLRQGNEEKEVEVLALSDQRRGAPEAQLLYSETEKSIKHREA
                     MVFARKANALSMPHPDRRPNKKERRDLIKFKEQEFS"
     gene            11455..12348
                     /gene="hslO"
                     /locus_tag="PWX02_000908"
     CDS             11455..12348
                     /gene="hslO"
                     /locus_tag="PWX02_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDL1728937.1"
                     /translation="MNYTKDNDKLYRYLFKDRAVRGEWVRLNNTFTETLNTHHYPKAV
                     QNLLGEMLVATSLLTATMKFNGNITVQIQGDGPLKLAVVNGNDQQQLRALARVQADIA
                     DTASLAEMIGNGVLVISIIPTDGERYQGVIALDKPTIRECLEDYFARSEQLQTHLVIR
                     TGEFEGKAVAGGMLLQIMPDGTGSRDDFEHLATLTETVKDEELFGLEAEELLYRLYHE
                     EQVEVYPPQDTEFKCGCSRERSGSALLLVPAEEIEEMLEEKQGVIDMQCECCGTQYFF
                     DKAAIEGFKEETEKLSKLGLN"
     gene            complement(12422..13060)
                     /locus_tag="PWX02_000909"
     CDS             complement(12422..13060)
                     /locus_tag="PWX02_000909"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDL1728938.1"
                     /translation="MQNKIDWQKVDGLLPVIVQNAQTCEVLMLGYMNQQALEKTLAEK
                     RVTFYSRTKQRLWTKGETSGNFLNVVDMSLDCDNDTLLILANPIGETCHTGAESCFHQ
                     FEQEQPDWIFFSKLERMIAERKNADPESSYTAKLYAKGTPKIVQKVGEEAVESVIAAM
                     KNDREELVGEVADLMYHLTVLLHNADLSWAEINAKLKERHKGIGLHPEGSNK"
     gene            complement(13087..13473)
                     /locus_tag="PWX02_000910"
     CDS             complement(13087..13473)
                     /locus_tag="PWX02_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL1728939.1"
                     /translation="MIKITALDHLVLTVADMQKTIDFYTTILGMQEIAFGDNRKALLF
                     GTQKINLHQKGAEILPNAQNADCGTADLCLLTVTPLQEVIYVLQRHQVEILEGGIVPR
                     TGAVGKIESVYCRDPDGNLIEISRYV"
     gene            complement(13470..14243)
                     /gene="hisF"
                     /locus_tag="PWX02_000911"
     CDS             complement(13470..14243)
                     /gene="hisF"
                     /locus_tag="PWX02_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDL1728940.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAQRYAQEGA
                     DELVFYDITASSDGRTVDKSWVERVAQVIDIPFCVAGGIKTIADAEQIFAFGADKISI
                     NSPALANPELITELAKRFGVQAVVVGIDSWFEAETGKYWVNQYTGDEKRTRQTEWQLL
                     DWVEEVQKRGAGEIVLNMMNQDGVRNGYDLVQLKKVREVCQVPLIASGGAGEMVHFRD
                     AFIEANVDGALAASVFHKQIINIGELKEYLAKEGVEVRR"
     gene            complement(14225..14974)
                     /gene="hisA"
                     /locus_tag="PWX02_000912"
     CDS             complement(14225..14974)
                     /gene="hisA"
                     /locus_tag="PWX02_000912"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006995165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDL1728941.1"
                     /translation="MQKSIIIPALDLIDGQVVRLHQGDYAKQTTYADDPIAQFADYLA
                     QGAEQLHLVDLTGAKNPAQRQTALIGKIIRETHCKIQVGGGIRTEQDVADLLAVGANR
                     VVIGSTAVKERETVKGWFKKYGAEKFVLALDVNIDAIGQKIIAISGWQEASGVSLEEL
                     IEDYQTVGLQHVLCTDISRDGTLAGSNVDLYREICAKYPDIQFQSSGGIGSLADIAAL
                     KGTGVAGVIVGRALLEGKFNVAEAIECWQNG"
     gene            complement(15144..15989)
                     /locus_tag="PWX02_000913"
     CDS             complement(15144..15989)
                     /locus_tag="PWX02_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor protein"
                     /protein_id="HDL1728942.1"
                     /translation="MNEIKLFENSQIRSVWDSEKEEWFFSVVDIIEVLTQSSNPRRYW
                     SDLKRKIKDEEGGIELYEKIVQLKLKAPDGKMRETDATDLQGIFRIIQSVPSPKAEPL
                     KMWLAEVGKERIDEIIDPELTIDRALATYLKKGYSREWINQRLQAIQVRKELTDTWQD
                     HGVQAGREFAILTNEITQAWSGMTTRQYKDFKGLKKESLRDNMTTTELVLNMLAEAAT
                     KDIAQISHPQGLEENKTVAKRGGNVAKVARESLEAETGKPVITQQNAIDFAAVIEQVA
                     KQVKK"
     gene            complement(16088..16681)
                     /gene="hisH"
                     /locus_tag="PWX02_000914"
     CDS             complement(16088..16681)
                     /gene="hisH"
                     /locus_tag="PWX02_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDL1728943.1"
                     /translation="MTNLTIIDTGCANLSSVKFAFDRLGIRAEISRDLNKIQSADKLL
                     LPGVGTAAAAMAILHDRRLIDTIRNATQPMLGICLGMQLMTEFSHEGDVATLNLMSGK
                     TERLPECGLPLPHMGWNKVQYQADHPLFHDIEQGNHFYFVHSYGVLPNENTIATCDYG
                     VPFSAAIASQNFYGVQFHPERSGEAGAKLLRNFVENV"
BASE COUNT         4658 a         3931 c         3513 g         4814 t
ORIGIN      
        1 gatctttttt ataagattaa gctagtgaaa aaattattaa acaagagaaa acaaaaccaa
       61 tgacaaaaca attcacaatg gaagaccgtt ttaacccttc cgctgtagag caagcgctct
      121 accaacactg ggaatcgcaa ggctatttta agccgagtga agatattaat gcaccgagct
      181 actgtatcgc tattccgccg ccgaatgtga cggggagttt gcatatgggg cacgctttcc
      241 agcaaacctt gatggatacg ctgattcgtt ttaaccgtat ggagggcaac aatacccttt
      301 ggcaaacagg aactgaccac gcagggattg ccactcagat ggtggtggag cgtaaaatcg
      361 cagcggaaga aggtaaaacc cgccacgatt atgggcgtga agcctttatc aacaaaatct
      421 gggattggaa agcctattcg ggcggcacga tcagccagca aatgcgtcgc ttaggcaact
      481 cgattgactg ggatcgtgag cgttttacga tggacgaagg cttatccaat gcggtaaaag
      541 aggtgttcgt tcgcttgcac gaagaaggct tgatttaccg tggcaaacgc ctcgtaaact
      601 gggatccgaa actgcatact gcgatttctg acttggaagt ggaaaacaaa gaaagcaaag
      661 gctcgctgtg gcatttccgc tatccgctgg ctaatggtgc aaaaaccgca gacggcttgg
      721 attacttagt ggtggcaacg acccgtcctg aaacgatgtt gggcgatact gcggtggcgg
      781 ttcaccctga agatgagcgt tatcaagctc tgattggcaa aagcgtaatc ctaccgcttg
      841 ccaaccgtga aatcccgatt attgccgatg agtatgtgga tcgtgagttc ggtacgggcg
      901 tggtaaaaat cacccctgcc cacgatttca acgactacga agtgggcaaa cgccacggct
      961 tgccgatggt gaatgtgatg accttaaatg ccgatattcg tgcagaggcc gaaattatcg
     1021 gcacagacgg taaaccgctt tccgactaca ccgccccaat tcctgccgac tatcaaagct
     1081 tagagcgttt tgccgcgcgt aagaaaattg tggcagattt tgaagctctt gggctgttag
     1141 atcaaatcaa accacacgac ttaaaagtgc cgtatggcga ccgtggtggc gtgccgattg
     1201 agccgatgct gaccgaccaa tggtatgtgt ctgtcaaacc tttggcggaa gtcgcgacca
     1261 aagcggtgga agacggcgaa atccaattcg tgccgaaaca gtacgaaaac ctgtatttct
     1321 cttggatgcg tgatattcaa gactggtgta tctctcgcca attatggtgg ggacaccgca
     1381 ttccagcgtg gtatgacgag cagggcaatg tttatgtagc acgagatgaa gcggaagtgc
     1441 gtgcgaaaca caacttacct gcggacttac cattaaaaca agatgaagac gtactggata
     1501 cttggttctc gtctggtcta tggacgttct caacgttagg ctggccagaa caaaccaaag
     1561 aactgaaaat gttccacccg accgatgtgc tgattaccgg ttttgacatc atcttcttct
     1621 gggtggctcg tatgattatg ttcacgatgc acttcatcaa agatgaaaac ggcaaaccgc
     1681 aagtgccgtt caaaaccgtg tatgtaacag gcttgatccg tgacgagcaa gggcagaaaa
     1741 tgtcgaaatc gaaagggaac gtgttagacc cgattgatat gattgacggc atcagtctcg
     1801 acgatttgct cgaaaaacgt acaggtaata tgatgcagcc acaactggca gaaaaaatcg
     1861 ctaaggcaac ccgcaaagaa tttgccgaag ggattgtggc acacggcaca gacgctctgc
     1921 gtttcaccct caccgccctt gccagcaacg gtcgagacat caactgggat atgaaacgtt
     1981 tagaaggcta ccgcaacttc tgcaacaaac tgtggaatgc gagccgttat gtgctgacca
     2041 atgacaagtt ggatttaagc gaaggcgacg tggaatttag ccttgccgat cgttggattg
     2101 aaagccaatt caaccgcacg gtggaaactt tccgcaccgc actcagccaa taccgtttcg
     2161 acttagtggc aaatgcgatc tacgaattta cttgggatca attctgcgac tggtatttag
     2221 agctgaccaa acctattttc ttcaaaggca cagatgtaca acgccgtggc gcaagtcgca
     2281 cgctggtgaa tgtattggaa aaactcctgc gtttaatcca cccagtaatg ccgttcatca
     2341 ccgaagaaat ctggcagaaa gtgaaaggct ttgtaggtat tgaagccgat accattatgt
     2401 tgcaaaaatt cccacaattt gacccgcttg ccattgatga aaccgccgaa agtcaaatca
     2461 actttatcaa agaagtgatt gtggcggtgc gtaacattcg tgcggaaagc aacatcgcac
     2521 caagcaaagg tttagattta atcgctcgta atttctctgc ggacgaagtc tctattctca
     2581 acgctaacga agtgttgctc aaatcgatgg caaaacttga tagcgtgaaa gtgctggaaa
     2641 atggtgaaaa tgcaccgcta tcggtagcga aactggtcgc aaatggtgag atcttgatcc
     2701 caatggcagg ctttatcaac aaagaagctg agctggctcg cctcaccaaa gagatggata
     2761 aacttaaagg tgaagtggct cgcattgaag gcaaactttc aaacgaagcc tttgtcgcca
     2821 aagccccaga gcaagtcatc gccaaagagc gtgaaaaaat gcaagagtac ctgagtggat
     2881 tagagaaatt gcaggtgcag tatcaagaga ttgaagcact gtaattaact aacaagcggt
     2941 caggatttgg aaattttttg ctaattcaga ccgcttgctt attgaagctt ttcattataa
     3001 atgtttggtt aaaatcgcct ctaggcggtc atagcctgct tgggtgaagt gtaatccatc
     3061 gtggcataaa gttagatcta aattcccttc tttgtcagca aaatattccc acgtttttac
     3121 ataagtcaaa tcagatggaa tatttgcctc aaaatacgca ttgagctctt ttattgcagc
     3181 attattggtg gtggtaatat tattaaccgg tgtggcttcc aatagaaaaa agttggcatt
     3241 tggattaatt ttgcgaagag aatccacaat ttcgccaatc cactctaaca cttgttttgg
     3301 cgagtaatcc ggctctttta caatgtcatt aacgcctaga aaaataaata cattatcgcc
     3361 aacgtgttga attcgctctt gtcgggtaat cacatccaaa tattggcggg tgctggtgcc
     3421 tgataagccc aaattggcaa ctgtttttcc tgctaaattc agcgtgccgt tttccatatc
     3481 gccccacata tcaaacaggg aatgcccaat aagggaaata tctgcctgtt tttcaaattc
     3541 tgattttttt gtttggtagc gattaaaaat atcttgatcg gtaagcatag gttcttctta
     3601 aatataaatg aaaaatcgct ccattattat acaaacttgt aaacattcac tccaagatat
     3661 tttgaagaaa aatccaataa aaaagccact taatatcgaa ttaagtggct caaaaagatt
     3721 aattatacat cataagtggt cgatgcggta ttaccgccac gtcctgtcca gttggtgtgg
     3781 aagaactcac cgcgagcttt gtctgtacgc tcataagtat gagcaccaaa gtagtcacgt
     3841 tgtgcttgga gtaagtttgc cggtaaacgt gccgaggtgt agccatctaa gaaggtaatg
     3901 gctgacgcca tacaaggtac aggtaaaccg atttccaccg attttgccac tactttacgc
     3961 caatctgcca agcagttttc caaaatatct ttgaagtatg ggtctgagcc taagaacact
     4021 aaatatgggt tggcttcata cgcatcacga atgttgccta agaaacggct gcggataata
     4081 caaccttcac gccataatag ggcggtattg ccgtagttga tgtcccaacc gaattgctca
     4141 gaagcctcac gaatcaacat aaagccttgt gcataagaga tgattttgga agcaagtaac
     4201 gcacggcgta ccgcttctac ccatactttt ttgtcgcctt caactttacc aatttctttg
     4261 ttaaacaatc ttgaagccgc aacacgttga tctttaaacg cagatacgca acgggcgaat
     4321 accgactcgg taatcagtgt taatggaatg ccgaaatcaa gggcgttaat accagtccat
     4381 ttacccgttc ctttttgacc tgcggtgtcg agaattttct ctaccaatgg ctcgccgtct
     4441 tcgtctttgt aggcaagaat atcagtggta atatccacca aatagctgtc taattcggtg
     4501 tttttccact ctttaaagat ttcgtgcatt tcatcatagc ttaaacctaa accatctttt
     4561 aagaattggt aggcttcaca aattaactgc atatcgccat attcgatacc gttgtgaacc
     4621 attttcacaa aatgccctgc tccatcacga ccaacccagt cacaacaagg ctcacctttg
     4681 tcggttttgg ctgaaattgc ttggaaaatc ggcttcacat attgccacgc agattcatca
     4741 ccgcccggca taattgacgg tccgtgacga gcaccctctt cgccgcccga tacacctgag
     4801 ccgataaagc gaatgccttt ttccgctaac gctttcacac ggcggttggt gtccggatag
     4861 tttgagttac cgccatcaat gatgatgtcg ccttcttcta aatgtggcag aagagcctcg
     4921 ataaactgat ccaccacctc accggcacgc accattagca tcactttacg cggtttttcc
     4981 aacttcgccg ctaaatcttc taacgagtaa gcaccgataa tgttcgtgcc tttcgccgca
     5041 ccttctaaga actcatccac ttttgaagta gtgcggttgt acgccaccac tttaaagccg
     5101 ttgtcattca tattcaaaat taaattttga cccattacgg cgagcccaat cacgccgata
     5161 tcgccttttt gtgacattgt ttgctcctat tgtttttgat tctatgcaag cggtcgaatt
     5221 ggtaaaattt tttgcaaatt tgaccgcttg taacatttat taacctaaaa taaactccac
     5281 cgccatttgg ctgtgtaatg ctgctgtatc gaaaaacggt aacggattgt cgttttggtt
     5341 aatcagcaac ccgatttcgg tacaacctaa aatcacacct tctgcacctt gttcactaag
     5401 ttgttgaata gtctgaagat aaaacgcttt ggcactttca gtaatcttgc ctaaacagag
     5461 ttcctcgaaa ataatgcgat gaatttcttg ttgcgtttcc tcatctggta ctattgatgt
     5521 gataccacgc tccaataaac cgtctttgta aaaattatca ctcatagtaa attttgtgcc
     5581 aaggagtgct actttctgca gaccttgagc tgaaatcgcc tcagccacac agtccaaaat
     5641 atgcaaaaac ggcacagaga tatggctact aatttgatcg gcaattttgt gcattgtgtt
     5701 ggttgccaat aaaatgcctt ctgctccggc ttgttctagc aatctagctt gttcagataa
     5761 aatttcagcg gctgcttgcc aatttccctg cttttggcat tgtacaatct cttcaaaatt
     5821 gacactcgaa attaaaatct tggcactgct atttccacct aatactttat tcaccgagcg
     5881 attaatatct aaataataag aaaccgtgct ttccgcactc attccaccta aaatgccgag
     5941 tgttttcatt aatatacctt attgattttc cgctaacttt tctaaaatgc ggctcattga
     6001 taaatccatt ttagaaaaag cgaaccattt cttacctaaa tctttaagtg atgcaccgat
     6061 atgataaagc tctttttcat caataatcaa aaatcggtcg tgagatttat caaatatttt
     6121 cacattaatg tgagcatatt ggctgttata tttttccaaa tctaatgcaa gttgcttaga
     6181 tacttgtttc gtataaatcg taactgatac gttatgcgat cgtttcgtaa ataaagttaa
     6241 aacggtatca tctacatagt tatcaattaa aatcagcgat gattttgcac ctctcactaa
     6301 ttccgtaaca aactgataag cgttaaaggt ttcaccttca aagaaaaccc cttgcatttt
     6361 aggcttattg ccttcaagag attgaaacac ctgctctaac tacccagaca tctccaactg
     6421 tttacgctct acactttcta agcgttgtac caaaccacta tttgtttgca aaagatgacg
     6481 catttgcaca aaggcttgca taatcttaat gctaacttgc actgcaactt cactacgcaa
     6541 tactgccgaa agcattgcaa tgccttgctc ggtaaagaca taaggaagat atttcctatg
     6601 ttggccacgc ccactatttt cgtttaagat cacaaattgt gatcttaaag atagccattc
     6661 gttttctgtt aattgaaaac gaaatatgtc aggaaatcgc tcttcattcc gttttactgc
     6721 ttggttaaag gttcgagtat ctacctgata aagctctgcc aaatgaacat ctagcattac
     6781 ttgctcgcca cgaatagtaa aaattttaga gttaatgttt ggttcaatta attgaattgg
     6841 attcatcttt cttccttatt cttcagtgaa gatcactttt gtgatctcaa aataagaaag
     6901 actatgccaa tttttggcct ataccacgag cagatttttc tgctttttcg atgaatatca
     6961 caaaaatact ttgcaacatt tttctaaaat ttaaccgctt gtaaaggtca ctgattgtga
     7021 cctagagtaa gttagccgcc tcactatcca aataccattc cgttacccca ttttccgcct
     7081 gaattttggc ggcaggataa ggtaaatttt ctgccggagt ggtttggatt tcttttaaaa
     7141 tttccgcttt gcttgctcct gtgaccaagt aagtgatccg tttggctttt tcaattaaag
     7201 atgccgtttt ggaaatacgg atctgccctg tttctgggtg tttggcgatc acggcaacgt
     7261 tctgatcatt aaaatctgtt tgatgtggga acagtgaggc ggtatgcccg tctgttccca
     7321 ttcctaaaat aatccaatca aacgccaagt tcggcacaca agcggtcaat tctgccgaaa
     7381 atcttgcaag ttcttgttct acattctctt caccacgaat gcggtgaata ttttcgcttg
     7441 gaatgtcgat gtgatcgaac agtaatttct gcacttcgcc gtagttactt tcgggatcgg
     7501 ttggggcaac cattcgatca tccccccacc agaaatgcag gttttgccat tgaatttcgg
     7561 tattaaacgg tgcttgagct aaggttttga ataataattt gggggttgaa ccaccggaaa
     7621 gggaaatatg caccgggcgg ttttgatggc tgtattcaat aaactcttta gcaatttgtt
     7681 caaccgcagc ttgggcggtg gggaaaataa ctttattcat aatttgcctc gttagatcac
     7741 ccctctttag aaaagagggg aactgaaatt tacactttct tcttcattgt accggacggt
     7801 tttcgccata ctttgccgtg tctggcgatt aatttatctg cctcggttgg cccccaagtg
     7861 ccggattcat attcatacac acgcccacga ttagctttgt agcctaaaat cggttgcaca
     7921 aatttccaac aagcgtgtac agcatcagta cgggcaaata aggttgcatc acctttaagt
     7981 gcgtctaaga gtaagcgttc ataagcggtg agtaagcttg atgatgaact taaatcagaa
     8041 tagcggaaat ccattgagac ttctttcgct tcaaagcctg ctcccggttt tttcaagccg
     8101 aaacgcattg aaatgccttc gtccggttgc acacgaataa tcaatttatt ttccggagca
     8161 ttttggctaa ataccgggtg tggtgtagtt ttaaaatgaa ttacgatttc cgtcacacgg
     8221 gttggtaagc gtttaccggt tcgcacatag aacggcacac ctgcccaacg ccagttatcg
     8281 atttcacatt ttaccgccat aaaggtttca gtgttggaat cagccggcac gcctttttct
     8341 tccaagtaag ccggcacttc tttgccgtct accgtgccac gagcatactg acctaacacc
     8401 acattgtgtt taacgtcttc ttcagttagc ggatgcaagc aatatagcac ttttgcgact
     8461 tcatcacgca ttgaattagc attaataatt gccggtggct ccattgcaac catggctaat
     8521 acttgcaata agtggttttg gaacatatca cgcattgccc ccgagccatc atagtaaccg
     8581 ccacgatctt caacgccgat ggattctgcc ccggtaattt ccacataatc aataaaatta
     8641 cggttccaaa gcggttcaaa taagccgtta gaaaaacgta aaacgagtaa gttttgtacc
     8701 gtttctttac ctaaatagtg gtcgatacgg tagatttgat gctcttcaaa gcatttgtgg
     8761 atttgtacgt ctaatttttt ggcggtttcg atatcgtagc cgaatggttt ttcaacaata
     8821 atccgtttcc agccaaactc ttcggtagtt aagccgtgag cggctaaaca ttccggaatc
     8881 acgccatata aacttggtgg cgtggagagg taataaagcg tgttaccggc tgtttgatat
     8941 ttatcgtgta gctcatcaag acgaggcagc aacttggcat aatccactgc atcagaggta
     9001 tttacagctt ggtaataaag atgttcgcaa aatttatcta attcttcacc gcttgctttt
     9061 tcaaatttga ctaacgcatc acgcattttt tggcggaaag attcgtcggt taattctgaa
     9121 cgagaaacac ctaggacgga aaaatgctcg cccaatctgc caatcttata taagttataa
     9181 agagcaggaa ttaatttacg aaaagttaag tcaccggaag caccaaaaat gacgatacag
     9241 ctattttcag cattcattta tttatcctat tgctaattgt taaaattttg ttgggtgcaa
     9301 gattatcata aaaattgcta atcggaacta cctatttgaa taaaaatatt ttatcccaat
     9361 ctatcgagcc gtcagccacc atcacaaaat aaggattaat caaactttct cgctgattat
     9421 aagtcagcgg cgaaaaatta aagtcgaaaa ttttgccgcc catttcactt aacagcactt
     9481 ctgccacagc agtatcccat tcgccggtgt caccaaaacg gatataacaa tcggctttcc
     9541 cttctgccac taaaccggct tttagactgc ccgaaccata ttttacacac tcaatttgtg
     9601 gggctgaaat ggcattttta gcttgttctg catgtaaatc gctgcctacg gtaacaatca
     9661 aaagggcatt ctccgcacgt ttcggtttga caagcggtcg aatttctcca ttttcttgca
     9721 aaaagctacc gctaccttcc atactaaaat aggtttttcc taatatcgga gcatgaataa
     9781 cgcccagcac cggctgattt ctaccctcac gtttttgcac taacgcaatc accactgaaa
     9841 attgaccggt gcggttaata aactgttgtg tgccgtcaat cgggtcgata atccaatatt
     9901 cttgccattg ttggcgttcg gcaaacggaa tatcgcaaaa ctcttccgat aataccggaa
     9961 tatctggtgt gagttgcttc aagcgttcag ttaaaaattg gctgataatc agatccgctt
    10021 ccgtcaccgg tgtgcaatcg gctttgattt taattgccac cgagcgggca taaagctgct
    10081 ttaactgctc accggctgct ttggcaatat tgataatatt ctcgagtagg ggttgagtta
    10141 gcgtttgcat aatagctcct tgcctatttt tttaagtatt ctcgtagcat aaatagagcg
    10201 gacaaattcc gtgcttcggc aaaatcagga tctgccaata attgatcaat tttatccaac
    10261 ggataacgca caatttccag cggttcaggc tcatcgcctt ccaattttga tgggtacaga
    10321 tcttgtgcca aaaacaggtg cattaagccg tacatatggc ttggcgaact gtaaagcgac
    10381 cgcagaaaat ctaatttttt cgccccaaaa ccaatttctt cttgtaattc acggttcgca
    10441 ctctcaatcg gctcttcacc tgcatccaca atgcctttcg gaaaacccag ctcgtaacgc
    10501 tccgagccga ccgcatactc tttcacaaaa atcagctcgt tgttttgaat cggaatcacc
    10561 attaccgaag aacggcgatt cggcgtaagt cgttcgtaag ttcgctcttc gccattagaa
    10621 aagcgtaaat cgaccgcttg cacctcaaaa atgcgagatt tcgctactgt tttaatgttc
    10681 aaaatttcag gaagttgttt tatcatttta tctcaccttt ttgagttatt ttagttgaaa
    10741 aatctcactt tcgctctaat atttcgacta ttgttttgat tttaaggcag aaaatgaaaa
    10801 ataaacacga aaaagaacaa aacgacgccg aagttcggct agataaatgg ctttgggcgg
    10861 ctcgttttta taaaacccgt tcgattgcca aagcaatgat tgaagggggt aaagtccatt
    10921 ataatgggca aagagctaaa gttagcaaag cggtggagat cggtgccatc atcaaacttc
    10981 gccaaggcaa tgaagaaaaa gaggtggaag tgttagccct cagtgaccaa cgccgagggg
    11041 ctccggaagc ccaactgctc tacagtgaaa ccgaaaaaag cattaaacac cgtgaggcaa
    11101 tggtatttgc ccgcaaagcg aatgctcttt caatgccaca ccctgatcgc cgtccaaaca
    11161 aaaaagaacg gcgggattta atcaaattca aagagcaaga atttagctaa gcccttgcaa
    11221 tcagaaaaat aatccctata ttgaatatcc taatgtaggg gcgaaaaatt tttcgcccct
    11281 atataattat ttcgggcgaa tattgattcg cccctacagc gttcgatttg cacattttta
    11341 gccacaaaat gcattgcatt ttgaacgttg gctttgccaa cggctccgta ggagcgaaca
    11401 aaattagaaa taattttgtg agtaataatc gaccgcttgc aacgaggaat caaaatgaat
    11461 tacaccaaag acaatgacaa actctaccgc tatttattca aagaccgagc ggtacggggc
    11521 gaatgggtgc gtttaaacaa cacctttacc gagaccttaa acacccatca ctaccctaaa
    11581 gcagtacaaa atttactcgg agaaatgtta gtcgcaacca gtttgctgac tgcaacaatg
    11641 aaatttaacg gcaacattac tgtgcagatt caaggtgacg gcccgttaaa attggcggtt
    11701 gtgaacggta acgaccaaca gcaactccgt gctttagccc gtgttcaggc agatattgca
    11761 gacacagcct cgttagcgga aatgatcggc aatggtgtat tagtgatttc tattatccca
    11821 accgatggtg aacgttatca aggggtgatt gctctcgata aaccaaccat tcgtgaatgt
    11881 ttagaagatt acttcgcccg ctccgagcaa ttacaaaccc acctcgttat ccgcacaggc
    11941 gaatttgaag gcaaagcagt ggcaggcggt atgttgctac aaattatgcc ggacggcaca
    12001 ggctcaagag atgattttga gcatttagcc acgctcactg aaaccgtaaa agatgaagag
    12061 ttatttggtt tagaggcaga agaattgctc tatcgcctct accacgaaga gcaagtagaa
    12121 gtttacccac cacaagatac tgaatttaaa tgtggctgct caagagagcg ttcaggctcc
    12181 gcattattac tggtgccggc agaagaaatc gaagaaatgt tggaggaaaa acaaggcgtg
    12241 atcgatatgc aatgtgaatg ctgcgggaca caatatttct ttgacaaagc ggcgattgaa
    12301 ggctttaagg aagaaactga aaaattaagt aagttagggt taaattaagc taaacaagcg
    12361 gtcaagtttc ttgtcttttt tgcaatttgt aaaaaaacgg tgaaatttga ccgcttgcat
    12421 tttacttatt tgagccttct gggtgtaacc caatcccttt gtggcgttct tttagtttag
    12481 cattgatctc tgcccacgag agatcggcat tgtgcaataa caccgttaag tgatacatca
    12541 gatcagccac ttcaccgacc aactcttccc gatcattttt cattgccgca atcacgcttt
    12601 ctactgcctc ttcccctact ttttggacga tttttggcgt gcctttggcg taaagtttag
    12661 cggtataaga actttccgga tcagcatttt tacgttcggc tatcattcgt tctaatttgc
    12721 tgaaaaagat ccaatctggt tgttcttgtt caaactgatg aaaacagctc tccgcccccg
    12781 tatggcaggt ttcgccgata gggttagcga ggattaacag ggtgtcgtta tcacaatcca
    12841 aactcatatc caccacgttt aggaaattgc ccgaggtttc gcctttcgtc cacagccgtt
    12901 gtttagtacg ggagtaaaaa gtcactcgct tttcagccag cgttttttcg agggcttgtt
    12961 ggttcatata tcctaacatc agtacttcgc aggtttgggc gttttggacg ataacgggca
    13021 gtaagccgtc cactttttgc cagtctattt tattttgcat tttttcttcc ttttttgacc
    13081 gcttgtttag acatatcggc ttatttcaat caaattacca tcagggtcac ggcaataaac
    13141 cgactcaatt tttcccactg cccccgtacg aggcacaatc ccaccttcca aaatctccac
    13201 ctgatgtcgc tgtaacacat agataacctc ctgaagtgga gttaccgtta agaggcataa
    13261 atctgccgtg ccacaatcgg cattttgggc attgggtagg atttctgcac ctttttggtg
    13321 gaggttgatt ttctgtgtgc caaacaaaag cgctttgcga ttatcgccaa aggcgatttc
    13381 ttgcataccc aaaatggtcg tataaaaatc aatggttttt tgcatatcgg caacggttaa
    13441 aaccagatga tctaaggcag taatcttaat catctacgca cctcaacccc ttccttcgcc
    13501 aaatattcct tcaactcccc aatattgata atttgtttat gaaacacact cgctgccaac
    13561 gcaccatcca catttgcctc aataaaagca tcacggaaat gcaccatttc gcctgcaccg
    13621 cctgaggcga tgagtgggac ttggcaaact tcacgcactt ttttcagttg caccaaatcg
    13681 tagccgttgc gtacgccgtc ttggttcatc atattcagca cgatttcgcc tgctcctcgc
    13741 ttttgcactt cctccaccca atccaacaat tgccattcgg tttggcgggt gcgtttttca
    13801 tcgccggtat attgattgac ccaatatttg ccggtttctg cctcaaacca gctatcaata
    13861 ccaaccacca ccgcctgcac gccaaagcgc tttgccagtt cggtaatcag ctcggggttg
    13921 gcaagggctg gggaattgat ggagattttg tccgctccga aagcgaaaat ctgctccgca
    13981 tcggcaatgg ttttgattcc gcctgccaca cagaacggaa tatcaatcac ctgtgccaca
    14041 cgttccaccc agcttttatc caccgttctg ccgtcacttg aggcggtaat atcgtaaaac
    14101 accaactcat ccgccccctc ttgggcataa cgctgggcga gtggcacaat gtcgccgatg
    14161 atttcgtggt tgcggaactg cacgcctttc acaacttgcc cgtcacgcac gtctaaacaa
    14221 ggaattatcc gttttgccaa cattcgattg cctccgctac attaaattta ccttccaaca
    14281 acgcacgacc gacaattacg ccagccacgc ccgtgccttt taaagcagca atatctgcaa
    14341 gtgagccgat accgcctgag gactgaaatt gaatatctgg gtatttggca cagatttctc
    14401 gatagagatc cacatttgaa cctgccagtg tgccgtctcg tgaaatatcg gtgcataaaa
    14461 cgtgttgcaa gcccacggtt tgataatctt caatcaactc ctcgagcgac acaccacttg
    14521 cctcttgcca gccgctaatc gcaatgattt tttgaccgat tgcatcaatg ttcacatcca
    14581 gtgccagcac aaatttctcc gcaccgtact ttttgaacca gcctttaacc gtttcacgct
    14641 ctttcaccgc agtcgaacca atcaccacac ggtttgctcc aacagcaagt aaatccgcca
    14701 catcctgctc ggtgcggata ccgccgccca cttgaatttt gcagtgggtt tcacgaataa
    14761 ttttgccgat aagtgcggtc tgtctttgag ccggattttt cgcccctgta agatcgacca
    14821 aatgtaactg ctccgcccct tgtgccaagt agtcggcaaa ttgggcaatc gggtcatcgg
    14881 cataagtcgt ctgtttcgca taatcgccct gatgcaggcg aactacttgc ccatcgataa
    14941 ggtcgagggc ggggataatg atggattttt gcatattatt gttcctgtgt gcctagcacg
    15001 agccgtgcta ggctgaataa tcccagtcgc gctgcgactg cctctctaag ctacggagta
    15061 gcttggttta tctagcccca tacggctcgt atggggtaca agcggtcaaa ttcttccaaa
    15121 aacttgcaaa aaaccgaccg cacttatttc ttcacctgtt tcgccacttg ttcaatcacc
    15181 gcagcgaaat caatcgcatt ttgttgcgta atcaccggtt tgccggtctc tgcctccaaa
    15241 ctttcccgag ccacttttgc aacatttccg ccccgtttgg caacggtttt attttcttcc
    15301 aagccctgtg ggtggctgat ttgagcgatg tctttggtgg cagcctcagc aagcatattc
    15361 agcaccaact cggtggtcgt catattgtct cgcagacttt cctttttcag ccctttgaaa
    15421 tccttatatt ggcgagtagt cataccgctc cacgcctgtg tgatttcatt ggtgagaatc
    15481 gcaaattctc tgcccgcttg cacaccgtga tcttgccaag tatccgtcag ttcttttcgc
    15541 acctgaatgg cttgcaagcg ttggttaatc cactcacggg aatatccctt tttcaagtaa
    15601 gtagccagag ctcggtcaat ggtcaattca ggatcgataa tctcatcaat ccgctctttg
    15661 cctacctctg ccagccacat tttcaacggc tcggctttcg ggcttggcac agactgaata
    15721 atacggaaaa tgccttgtaa atccgtggca tcggtttccc gcatttttcc gtctggggct
    15781 tttaatttca actgtacgat tttttcgtac agttcaattc cgccctcttc gtccttgatt
    15841 ttccgcttta aatcactcca ataacggcgt ggattgctac tttgcgtcag cacctcgata
    15901 atgtccacca ccgagaaaaa ccactcttct ttttcgctat cccacaccga gcggatttgg
    15961 ctgttttcaa atagtttaat ttcgttcatt tctcaccttc tttcactggt taaattaaca
    16021 gtaattttgt cgaaataaag cgagaaatca agccctattt tcaaactttt cgatcgagat
    16081 cacaaaatca gacattctcc acaaaattcc tgagcaactt cgccccagcc tcgcccgaac
    16141 gctcggggtg gaactgtacg ccgtagaagt tttggctggc aatggcagcg gaaaacggca
    16201 cgccgtagtc gcaggtggcg atggtgttct cattcggtag cacaccgtag ctatgcacaa
    16261 aatagaaatg attgccttgc tcaatgtcgt gaaatagcgg atggtcggct tggtattgca
    16321 ccttgttcca gcccatatgc ggcaacggca aaccgcattc aggcaagcgt tcggttttgc
    16381 cactcattaa gttcagtgtt gccacatcgc cttcgtggga aaattccgtc attaactgca
    16441 ttcctaagca gatgccaagc atcggctggg tggcgttgcg aatggtgtcg ataagtcggc
    16501 gatcgtgcaa gatcgccatc gccgccgctg ctgttcccac accggggagt agcaatttat
    16561 cggcagattg gattttgttg agatctcgcg agatctcagc ccgaatgcca agacggtcaa
    16621 aagcaaattt gaccgaagac aggttggcac agcctgtgtc gatgatggtg aggttggtca
    16681 tttagtttcc tgagttaagt atttcttata gtgaagtaaa attgactcaa tatatctgtt
    16741 tccttcaaaa gctttcttat cgaatctttt gataattgcc aaaaataaac tattaaagaa
    16801 aaaatcagat ttaaaattat tatttagtaa atctatatct agttgctcac cttttagttt
    16861 tacacttaaa atagatttat aacattccaa taactctgga gttcttgttt catgca
//
LOCUS       DANJWC010000038        16889 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.038, whole genome shotgun sequence.
ACCESSION   DANJWC010000038 DANJWC010000000
VERSION     DANJWC010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16889
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.038"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..438
                     /gene="nudB"
                     /locus_tag="PWX02_000915"
     CDS             <1..438
                     /gene="nudB"
                     /locus_tag="PWX02_000915"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HDL1728944.1"
                     /translation="NYKNPHSILVVIYAKNTQRVLMLQRKDDPDFWQSVTGSINIGET
                     PLEAAEREVSEETGLQTFEQKQPLVDCQKQIEFEIFPHFRYKYAPNITHCVEHWFLLP
                     LDSEQEPILTEHLAYRWVSVEDAVRLTKSPNNAQAIQEYLTSK"
     gene            451..1191
                     /locus_tag="PWX02_000916"
     CDS             451..1191
                     /locus_tag="PWX02_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1728945.1"
                     /translation="MAGHSKWANIKHRKAAQDAQRGKIFTKLIRELVTAAKLGGGDTS
                     SNPRLRAAVDKALSSNMTRDTINRAIERGVGGGDDTNMETKIYEGYGPGGTAVMVECL
                     SDNANRTISQVRPSFTKCGGNLGTEGSVGYLFSKKGLILVANADEDALTEAAIEAGAD
                     DIQPQEDGSFEIYTAWEDLGSVKDGIEAAGFKIENAEVTMIPSTTVELDAETAPKLLD
                     LINRLEDCDDVQNVYHNGEISDEVAALL"
     gene            1321..1848
                     /locus_tag="PWX02_000917"
     CDS             1321..1848
                     /locus_tag="PWX02_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728946.1"
                     /translation="MKLVKFVPALAATLVLAACSNANTQSAPQQAQDAVTKAAEQATQ
                     QVNEIAGNAPRASGAVKVEKATDGAKSIVYRCQSGKRVTANYVFQGEEAKAVNLLVQS
                     GGKTQKIPTLTRDASNQDSTSFKTDKYSWDLDTGFTLSSNGQSGMLTKYGAKTDTIVA
                     KLCDVDKALTSKLNK"
     gene            1915..2493
                     /gene="ruvC"
                     /locus_tag="PWX02_000918"
     CDS             1915..2493
                     /gene="ruvC"
                     /locus_tag="PWX02_000918"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDL1728947.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQNGRQLEYLGSGAIRTTAEDLPTR
                     LKRIYAGVSEIITQFQPNMFAIEQVFMAKNPDSALKLGQARGTAIVAAVNHDLPVFEY
                     AARLVKQTVTGIGSADKVQVQDMVTRMLKLSAKPQADAADALAIAITHAHSIQHSLIV
                     AKQSQQNVSSEKEQILALMKTRYSRGRFRLKG"
     gene            complement(2559..3839)
                     /gene="murA"
                     /locus_tag="PWX02_000919"
     CDS             complement(2559..3839)
                     /gene="murA"
                     /locus_tag="PWX02_000919"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL1728948.1"
                     /translation="MEKFRVYGPFSLNGTVDISGAKNAALPILFAAILAEKTVTLKNV
                     PDLKDVDTTFKILRQLGVTVEKGSDKGVVHIDASKINNYVAPYELVKTMRASIWALAP
                     LVARFQLGQVSLPGGCTIGARPVDMHISGLEKMGAKIELDEGYVKATSNGRLNGARIY
                     MDKVSVGATLSVMMAGTLAKGTTVIENAAREPEIVDTADFLNAMGAKISGAGTDMITI
                     EGVEHLGGCEHSVVPDRIETGTFLVAAAISGGRITCRNTKADTLDAVIEKLREAGMQV
                     DVTENTITLDSLGRRPKAVNIRTMPHPGFPTDMQAQFTLLNTVAEGTSRITETIFENR
                     FMHIPELIRMGAKAEIEGNTAICYGVEQLKSAEVMATDLRASISLVLAGCIATGETIV
                     DRIYHIDRGYEHIEEKLRQIGARIERFSAPFEGE"
     gene            complement(3949..4209)
                     /locus_tag="PWX02_000920"
     CDS             complement(3949..4209)
                     /locus_tag="PWX02_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HDL1728949.1"
                     /translation="MNPSQIEEILKQALSDATEVHAQGENAHFGVIVVSDSIASLSKV
                     KQQQAVYAPLAEYFTTNAIHALTIKVFSVEKWKTERLLNMVG"
     gene            complement(4242..4565)
                     /locus_tag="PWX02_000921"
     CDS             complement(4242..4565)
                     /locus_tag="PWX02_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HDL1728950.1"
                     /translation="MPQKTLQWDIQQNNESLFVKLSGELTRNTLLPLWKQRASFLSPT
                     GKQSLYWDLKEITRIDSAGFTLLAELLNHYHKIIPNSLINTPDSVRTLAALYDLDGWF
                     EQFII"
     gene            complement(4577..5212)
                     /gene="mlaC"
                     /locus_tag="PWX02_000922"
     CDS             complement(4577..5212)
                     /gene="mlaC"
                     /locus_tag="PWX02_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HDL1728951.1"
                     /translation="MLKNIKKIIVTGLVAVSALFSTTAFAETSPYVLMQQTADKLFGD
                     IKANQAKIKSNPEYLRTIVRNDLMPHVHVKYAGQLVLGKNLSAATDAQKEAFFNAFGQ
                     FVEQSYAQVLTQYQDQSVQIESPKSVDGKSIVSIRVNVSGKGAAQPIKLDFKWRKNSK
                     TGEWQAYDMAAEGVSMVATKQNEWAGVIRQKGIDALIAQVAQSAKQPITLK"
     gene            complement(5258..5782)
                     /gene="mlaD"
                     /locus_tag="PWX02_000923"
     CDS             complement(5258..5782)
                     /gene="mlaD"
                     /locus_tag="PWX02_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HDL1728952.1"
                     /translation="MRQSIKYEFWVGLFVLLGLAALVFLGLRVANVQGFSNEKTYTLY
                     ATFDNIGGLKVRAPIKVGGVVVGRVADISLDNKTYTPKVALAVNESFNQIPDTSSLSI
                     KTSGLLGEQYVALNVGFVIEGETAMMKEGDTFVDTNSAMVLEDLIGQFLYGDKKSDKT
                     EDEKASDSAEPKAE"
     gene            complement(5803..6579)
                     /gene="mlaE"
                     /locus_tag="PWX02_000924"
     CDS             complement(5803..6579)
                     /gene="mlaE"
                     /locus_tag="PWX02_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HDL1728953.1"
                     /translation="MVNFISSIGAVVINFIRTLGRSAFMLWGALIGKPEFRKHTPLLI
                     KQLYVLGVQSLLIIMLSGLFIGMVLGLQGYVVLVDFAAESSLGTLVSLSLLRELGPVV
                     TALLFAGRAGSALTAEIGLMKATEQLSSLEMMAVDPLRRIIAPRFWAGVISMPVLAAI
                     FTAIGIWGGSLVGVDWKGVDGGSFWSVMQNSVNTSDLINGFIKSVIFAFAVVWIALFN
                     GYDCVPTSEGISQATTRTVVNASLVILGLDFILTAIMFGG"
     gene            complement(6572..7375)
                     /gene="mlaF"
                     /locus_tag="PWX02_000925"
     CDS             complement(6572..7375)
                     /gene="mlaF"
                     /locus_tag="PWX02_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HDL1728954.1"
                     /translation="MTKTENLVEVKNLTFKRGERVIYDNLNLQVQKGKVTAIMGSSGI
                     GKTTLLRLIGGQILPESGEILFDGRDICKANNKELYEIRQRMGMLFQSGALFTDISTF
                     DNVAFPIRERTRLPEELIRKLVLLKLEAVGLRGAANLMPSELSGGMARRAALARAIAL
                     DPDLIMYDEPFTGQDPISMGVIVELIKELNQALNLTSIVVSHDVKEVLSIADYAYIVA
                     DKRVIAEGTPEILQASEDPQVVQFLAGKADGPVRFHYPAKDYTEELFNG"
     gene            7600..8994
                     /locus_tag="PWX02_000926"
     CDS             7600..8994
                     /locus_tag="PWX02_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HDL1728955.1"
                     /translation="MNKTMKKSFLTAFILGSTIAAMPAYAEATLPTAVNGQAVPSLAP
                     MLEKVRPAVVSIAVEGKTKGDSRRSREIPEEFEFFFGPHADLFGNRSSAPRNFRGVGS
                     GAVINAEKGYVITNNHVIDNADKITVKLEDGREFKAKLVGADPLSDVALIQVEDPKNL
                     TEIKFADSDKLQVGDFTVAIGNPFGLGQTVTSGIISALGRSTGQSDEGYENYIQTDAA
                     VNQGNSGGPLINLNGELIGINTAIISPSGGNAGIAFAIPSNMANSLVTQIIEFGEVKR
                     GMLGIKGGELNADLAKEFNISTQQGAFVSEVLPDSAAAKAGFKAGDVITEINGQKIRS
                     FSELRAKVATSGVGKEIELTYLRDGKEAKAKVTLQSDSEAKVTASNLIPALKGADFNN
                     YNAKGIKGVEISNVEKGSIAEMRSLKKGDIIIGVNRHSVENIADLRKILETKPSVVAL
                     NIVRDGANFYVILQ"
     gene            complement(9046..9807)
                     /locus_tag="PWX02_000927"
     CDS             complement(9046..9807)
                     /locus_tag="PWX02_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C biogenesis protein"
                     /protein_id="HDL1728956.1"
                     /translation="MKTREQLSQENYQQAVKFAQHFADDVRQEFEREAEQRYQFERTQ
                     FEQSVLQNNAQNRPLAKIASLSCVAILLLAMTYYGLSGRYQVVQSGLQQHQDFQQQMS
                     DAHGTSKNERYILSLQNQLRENPNNGDLWYELGQAYALSNDFDSALVCYDNAEKLLGK
                     RAAILGAIATARYYDNGQKMTVEIQAIIEQALQLDKNENASLLLLASDSFLNNNFQQA
                     LDNWRKVLDSNNDSINRRAIIQSMEMARQMLNSQQ"
     gene            complement(9794..10246)
                     /gene="nrfF"
                     /locus_tag="PWX02_000928"
     CDS             complement(9794..10246)
                     /gene="nrfF"
                     /locus_tag="PWX02_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HDL1728957.1"
                     /translation="MRNSIISIGFLQKIRKILPLVFGLLLFVGTVQAEMVDTFQFQNE
                     ADRVRAISLARSLRCLQCQNQNLVESNATAAYNMRIEVYEMVNQGKSNEEIIRIMTER
                     FGSFVNYDPPFTPQTWVLWGLPVGLFSLLFLAILWRTSKNKGNKNENA"
     gene            complement(10236..10769)
                     /locus_tag="PWX02_000929"
     CDS             complement(10236..10769)
                     /locus_tag="PWX02_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HDL1728958.1"
                     /translation="MKKILLLIPLLLLVVIVGFLTVPLMNKDALAPTEDWQGKPFPEF
                     VGKNLLDSNAHLNNNSLPKEPYILNVWASWCTWCIKEFPILLELKQKGVPIVGLTYSD
                     RPNDALNALKNWGNPFSLIIDDYEKGFLIQTLKVSSAPSSYLIDKHGVVRYQQKGYNP
                     DFAQDFLPKLEALRNEK"
     gene            complement(10766..12670)
                     /gene="nrfE"
                     /locus_tag="PWX02_000930"
     CDS             complement(10766..12670)
                     /gene="nrfE"
                     /locus_tag="PWX02_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfE"
                     /protein_id="HDL1728959.1"
                     /translation="MLPELGYFSLLLAAISAIFQVGFALLGEVRKQYHWLALAPLFTY
                     LQAAFTTFSFSTLAYAFLTDDFSVIYVAAHSNSQLPDFFKFAATWGGHEGSMLFWLTA
                     LVIWSAVFCKFSQKIDRLFANRTLAMLGLIALGFLLFILLVSSPFERGFPPPPEGRDL
                     NPMLQDIGLIFHPPLLYLGYVGFAVSFAMIVAALVSGGMDAAIMRWLRPWTMISWGFL
                     TAGIILGAWWAYYELGWGGWWFWDPVENASLMPWLLGTALVHSLIVSQQRGIFYYWTI
                     LLAIFAFALSLLGTFIVRSGVLTSVHAFAVDPDRGMALLILFFGLSFIALALFAFRAN
                     LWQGKVRFNLLSKETAFLLINGLFSVAASVVLLGTFYPMIFTVMNWGSISVGAPYFNS
                     VFTPLALLLMIVMGFAVVLGWKQISNKQILVKLWLLPIAVGLALGLIYASISQREHFA
                     LQPLPTIFISLAIWIILTHLPYSQKMWQIRPLAMRLAHIGFAVCVIGAMMNSYYGDEI
                     GVRLKPNQAAELAGYTFKYEDYHDLIGPNYTAEQAVFSISRDEQLLATVTPERRYYDV
                     RTMTMAEVGLFHHNLDDIYIVMGDKFGNLEYAFRLHYKPYVQALWLGGMMMVMAALLA
                     LFGYRRENKK"
     gene            complement(12940..13887)
                     /gene="nrfD"
                     /locus_tag="PWX02_000931"
     CDS             complement(12940..13887)
                     /gene="nrfD"
                     /locus_tag="PWX02_000931"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HDL1728960.1"
                     /translation="MNEYVPFQTPNLVWDGTIAIYLFLLGISSGAVQLAIAYRNSGVK
                     MEKPSQNWIIRSAAILGTLPTIIGLLLLIFHLTKPWTFWKLMFNYNFTSVMSMGVMLF
                     QLYMAVLVVWIAIMFKDWIVRYLPVVSGLIDFAEAKLLKPIEFILFVLAAVLGAYTGF
                     LLSALVSYPMLNNPVLPALFLASGASSGIAATFLVILLAGKLSGESHEVHFMHKFEVP
                     IMVTELGLIVAFFVGLHFGGADKSLALTNALNGFWGNVFWIGVMLIGIAIPLVANIFG
                     SHRLKHNVKFIILISVFDLIGVLCLRYFILYAGQLTVAS"
     gene            complement(13887..14564)
                     /gene="nrfC"
                     /locus_tag="PWX02_000932"
     CDS             complement(13887..14564)
                     /gene="nrfC"
                     /locus_tag="PWX02_000932"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HDL1728961.1"
                     /translation="MTCTRRDFVSGAGAIAVVASTGLATSITLATEKEQKKIRYAMVH
                     DETACIGCTACMDACRTTNKVPEGVSRLEIIRSEPFGEFPNVQYEFFRQSCQHCTNAP
                     CVSVCPTGASFIDPETGIVDVHSDLCVGCQYCIAVCPYRVRFIHPEKKSADKCNFCRD
                     TNLAEGKQPACVEACPTKALTFGDMNDPSSDIFHKVKNNPVYRTKTALGTEPNLYHIP
                     FGKGEHR"
     gene            complement(14564..15220)
                     /gene="nrfB"
                     /locus_tag="PWX02_000933"
     CDS             complement(14564..15220)
                     /gene="nrfB"
                     /locus_tag="PWX02_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HDL1728962.1"
                     /translation="MKKLISKAGRAVALIQAVVFAGFFAISSANALMPEQPKPLWQSE
                     TADDAKVAQDARRDPNKYCTQCHESANATGKPFHHAGKHFQKDVKSPNNGEPLTCVSC
                     HGNISESHRKGAKDVMRFNPHGKASNPSLERSVNEQNQVCFACHTAEKLREKFWAHDT
                     HATKIACTNCHEIHPEKDPMKDIPEKDRIKLCTDCHTKIHSGEFKNPEMKATEAKGTK
                     "
     gene            complement(15239..16741)
                     /gene="nrfA"
                     /locus_tag="PWX02_000934"
     CDS             complement(15239..16741)
                     /gene="nrfA"
                     /locus_tag="PWX02_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HDL1728963.1"
                     /translation="MKFLIKSLAVATISILGCLQTALAEEAKTESLTDKAVKHEKLGV
                     KIESANHLFAEKYPLQYDSWKSTAKSTDRGSALEADPRYVILWAGYAFAKDYNKPRGH
                     FYAVTDVRDILRTGAPKDENDGPQPMACWTCKGPDVPRLIEEKGERGYFDPKWAKYGA
                     EIVNSIGCADCHDTTSEEFKQGKPALRVARPHVLRALNTVGWKFEDLDKHGKRPAVCA
                     NCHVEYYFKNKTDVTFPWDKGVDVDSIEKYYDEINFTDWTHALSKAPMLKTQHPDFEV
                     WSQGTHGKNGVTCIDCHMPKVKDKDGKVYTDHKIGNPFDQFEATCKTCHEQSKETLEK
                     RVKEYKHEVKEAMIRLEDQLVKAHFEAKAAWEAGATQEEMKEILMAIRHAQWRWDYSA
                     AGHGNHFHAPDVMLRTIATGIDRAADARAKLGVVLAKHGVTTPVAIPDISTKEKAQLA
                     IGLDIPKEQAAKDEFLKTVVPQWEKEARAKGLLPAEEADKPVAAPKAEAK"
BASE COUNT         5060 a         3742 c         3344 g         4743 t
ORIGIN      
        1 aactataaaa atcctcatag tatccttgtg gtgatttatg ctaaaaatac ccaacgagta
       61 ttgatgctac aacgcaagga cgatcctgat ttttggcaat cggtgaccgg ttcgatcaat
      121 attggagaaa caccgctgga agcagcagaa cgggaagtga gcgaagaaac cggtttgcaa
      181 acttttgagc aaaaacaacc gcttgttgat tgccaaaagc agatagaatt tgaaattttc
      241 ccgcattttc ggtataaata cgcacctaat atcacacatt gtgtcgaaca ttggtttttg
      301 ctgccacttg atagtgagca agagcctatt ttaaccgaac atttggcata tcgttgggtt
      361 tcagtggaag atgcggttcg actgactaaa tctcccaaca atgcccaagc aatacaagaa
      421 tatttaacat caaaataagg aaacaacaga atggcaggcc atagtaagtg ggcaaatatt
      481 aaacaccgta aagcggcaca agatgcacaa cgcggtaaaa tttttactaa attaattcgt
      541 gaattggtga ctgccgccaa actcggcggt ggtgatacct cttccaaccc tcgtttgcgt
      601 gcggcggtgg ataaagcgtt atccagcaat atgacccgtg ataccatcaa ccgtgcaatc
      661 gagcgtggtg taggcggtgg cgatgatacc aatatggaaa ccaaaatcta cgaaggttac
      721 ggcccgggtg gtacagcagt gatggtggaa tgtttaagtg ataacgctaa ccgtacgatt
      781 tcccaagttc gtccaagttt cacaaaatgt ggcggtaacc taggtactga gggttcggta
      841 ggttatttat ttagcaaaaa aggcttaatt ttagtggcaa atgccgatga agatgcctta
      901 actgaagctg ctattgaggc cggtgcggac gatattcaac cacaagaaga tggtagcttt
      961 gaaatttata ccgcttggga agatttaggc tcagtgaaag acggcattga ggctgcaggc
     1021 tttaaaattg agaatgcaga agtaacaatg attccatcaa ctaccgttga acttgacgca
     1081 gaaactgcac caaaacttct tgatttaatc aaccgccttg aagattgtga tgatgtacaa
     1141 aacgtttatc acaatggtga aatcagtgac gaagtcgctg cattactgta ataaaaaaat
     1201 cacgcaaaat aagcataatt taggttatgc ttatttttta tgaacttttt tcctatatta
     1261 ctgactaacc taatgtagtt tatatgctac tactttacta taacatttta aggagagtct
     1321 atgaaattag tgaaatttgt ccctgcccta gcagcaactt tagtattagc cgcttgctct
     1381 aatgcaaaca cacaatctgc tcctcagcaa gctcaggacg ctgtgactaa agcggctgaa
     1441 caagcaacac agcaagtgaa tgaaattgca ggtaatgcac ctcgtgcaag tggtgcagta
     1501 aaagtagaaa aagcaactga tggtgcaaaa tcaattgtat atcgttgcca aagtggtaaa
     1561 cgtgtaactg cgaattatgt cttccaagga gaagaggcaa aagcagtaaa tctattagtc
     1621 caaagcggcg gtaaaacaca aaagatccca acattaactc gtgatgcctc aaaccaagat
     1681 tctacttcat tcaaaacgga taaatattct tgggatttag acacaggctt taccttatca
     1741 tctaatggcc agtcaggtat gctaaccaaa tatggtgcta aaacagatac catcgttgct
     1801 aaattatgtg atgtagataa agcattaacc tcaaaattaa ataaataatc tttcttgatt
     1861 ttactactca taagcgagaa gataatatct tctcgcttat tttattgcac aaagatggct
     1921 attattttag gaattgaccc cggctcaagg gtaacaggtt acggtgtgat tcgccaaaac
     1981 gggcgacagc tggaatattt aggaagtggg gctattcgaa ccacggctga agatctgcct
     2041 acccgcttaa aacggattta tgcaggcgtg agtgaaatca tcactcaatt tcaaccaaat
     2101 atgttcgcca tagagcaggt atttatggca aaaaaccctg attctgcctt aaagctcggg
     2161 caggctcgag gaacagcgat tgtagcagcg gttaatcacg atttacctgt gtttgaatat
     2221 gccgcccgat tagtcaaaca aaccgttacg ggaattggtt ctgctgataa agtacaagtg
     2281 caagatatgg taacccgtat gcttaaactc tccgccaaac cacaagccga tgcggctgat
     2341 gcattagcca ttgcgattac ccacgctcat tcaatccaac attcactgat tgttgctaag
     2401 caaagccagc agaatgtgag tagtgagaaa gagcaaattt tagcactaat gaaaacccgt
     2461 tatagccggg gaaggtttag gttaaaaggc taactcaata taaaatgacc gcttggaact
     2521 aatgtttcaa gcggtcattt tttgagaaaa ttttacaatt actcgccttc aaaaggagct
     2581 gagaaacgtt caattctcgc cccaatctga cgtaattttt cttcgatgtg ttcatagcca
     2641 cggtctatgt ggtaaattct atccacaatc gtttcacccg ttgcaataca gcctgccaac
     2701 actaagctga tggaagcacg caagtcagtt gccattactt ctgctgattt taactgctct
     2761 acaccatagc aaattgcagt attaccttca atttccgctt tggcacccat acgaatcagc
     2821 tcaggaatat gcataaaacg gttttcaaaa atcgtttctg taatacggct tgtgccttct
     2881 gcaacggtat ttaataaggt aaattgagct tgcatatcag ttgggaaacc tgggtgtggc
     2941 atagtgcgaa tgttcactgc tttcgggcga cgacctagag aatcaagcgt gatagtattt
     3001 tccgtcacat caacctgcat tccagcttca cgtaattttt cgatcactgc atcaagagtg
     3061 tctgctttgg tgttacggca agtaattcga ccaccggaaa tagccgctgc cactaaaaat
     3121 gtaccggttt caatacgatc cggcactacg ctgtgttcgc aaccgcctaa atgctcaacc
     3181 ccttcaatgg taatcatatc ggtacctgca ccagaaattt tagctcccat cgcatttaag
     3241 aaatcggcag tatctacaat ttcaggctca cgagcagcat tttcaataac agtagtacct
     3301 tttgctagag tacctgccat catcaccgat aaagttgctc ctacgctcac tttatccata
     3361 tagattcttg caccgtttaa acgaccgttt gaagttgctt tgacatagcc ttcatcaagc
     3421 tcgattttcg cccccatttt ttcaagaccg gaaatgtgca tatctaccgg tcttgccccg
     3481 atagtacaac cgcccggtaa agaaacttgc ccaagttgga accgggcaac taatggagct
     3541 aacgcccaaa ttgaagcacg cattgttttc actaattcgt aaggtgcaac gtaattattg
     3601 atcttactcg catcaatatg taccacgcct ttatcgctac ctttttcaac cgttacccct
     3661 aattggcgta agattttaaa agtggtgtcc acatctttta aatcaggcac attttttaac
     3721 gtaactgttt tttcagccaa aatagcggca aataaaatag ggagtgcggc atttttcgca
     3781 cctgaaatat cgacagtacc atttaaagaa aatggaccat acacacgaaa tttttccatt
     3841 tgggagatcc taaaaaataa taatttgtag gggcgaaaaa tttttcgccc aattttccaa
     3901 cgtttacaaa agagtttggg cgaatagtga ttcgccccta catttcatct atccaaccat
     3961 atttagcaaa cgctcagttt tccatttttc cacgctgaat actttaatgg ttaaagcgtg
     4021 aatcgcatta gtagtgaaat actcagctaa aggggcataa acagcttgct gttgttttac
     4081 ttttgaaaga gaagcaatgc tgtcacttac tacaataacg ccaaagtggg cattctcgcc
     4141 ttgagcgtgg acttctgttg catcagataa tgcttgtttt aaaatttctt caatttgtga
     4201 tggattcatt ctgacttcct aaaatatgat tggtaactca ctcaaataat aaattgctca
     4261 aaccaaccgt ctaaatcgta taatgcagcc aaagttctga cagaatctgg cgtattaatc
     4321 aaactgtttg gtatgatttt atggtaatga ttcagtaatt cagcaagtaa ggtaaaaccg
     4381 gcagaatcaa tccgagtgat ttcttttaaa tcccaatata ggctttgttt ccctgttgga
     4441 gataaaaaag aagcacgttg cttccataaa gggagcaacg tgttacgagt taattcgcct
     4501 gatagtttca caaaaagact ttcgttattt tgctgaatgt cccattgtaa tgttttttga
     4561 ggcataacta aactacttat tttaaagtga taggctgttt tgcagattgt gccacttgtg
     4621 caattaaggc atcaatccct ttttgacgaa tcacacccgc ccattcgttt tgcttggttg
     4681 caaccatact tactccttca gcggccatat cgtatgcttg ccattcacct gttttgctgt
     4741 ttttacgcca tttaaaatct aatttgatcg gctgtgcagc acctttacct gacacgttta
     4801 cacgaatgct tacgatagat ttaccatcca ccgatttcgg gctttcaatt tgcacacttt
     4861 gatcttgata ctgggttaac acctgagcat aagactgctc aacaaattga ccaaaagcat
     4921 taaagaatgc ctctttttgt gcatcagtcg cagccgataa gtttttccct aacactaatt
     4981 ggcctgcata tttcacgtga acgtgtggca ttaaatcgtt acgcacaatg gtacgcaaat
     5041 attcagggtt tgatttaatt ttcgcttgat ttgctttaat atcgccaaat aatttatctg
     5101 cggtttgttg cattaataca taagggctag tttcagcaaa tgcggtggtt gaaaataatg
     5161 cagaaactgc cactaagcct gttacgataa tttttttaat gttttttaac attattgatg
     5221 ctctccaaaa ctctaaatta gaggttgttg tacttaatta ctctgctttt ggttcagcag
     5281 aatcacttgc tttttcatct tcagttttat ctgacttttt atcaccatat aagaactgac
     5341 cgattaaatc ttctaacacc attgcagaat tggtatctac aaaagtatca ccttctttca
     5401 tcattgcagt ttcaccttcg attacgaagc caacatttaa cgcaacgtat tgctcgccta
     5461 ataagcctga ggttttaatt gagagcgagc tggtatcggg aatttgatta aaactttcat
     5521 ttaccgctaa tgccactttc ggggtgtagg ttttgttatc aagtgaaata tcagccactc
     5581 ggccaactac cacaccgcca actttaatag gcgcacgaac ctttagtcca ccaatattat
     5641 caaaagttgc atacagggtg taagttttct catttgagaa gccctgcaca tttgctactc
     5701 ttaagcctaa aaagacaaga gctgcaagcc caagtagtac aaataagcct acccaaaatt
     5761 catatttaat tgattgacgc attgtctgtt cctttcaaaa aatcaaccgc cgaacattat
     5821 cgcagttaaa ataaaatcta atcctaaaat caccagtgag gcatttacca ccgttcttgt
     5881 ggtcgcttgg ctgattcctt ctgaagtcgg tacgcaatcg tagccgttaa ataaggcaat
     5941 ccagaccacc gcaaaggcaa aaatcacact tttaataaag ccgttaatta aatcgctggt
     6001 atttaccgag ttttgcatta ccgaccaaaa actgccacca tcaacgcctt tccaatctac
     6061 cccgactaat gagccgcccc aaattccgat agcggtaaaa atagcagcta acaccggcat
     6121 tgaaatcacg cctgcccaaa agcgaggagc gatgatacgt ctaagcggat caaccgccat
     6181 catttcaaga ctggaaagtt gctctgtggc tttcatcagc ccgatttctg cggtcaatgc
     6241 cgaaccggct cgccctgcaa ataataatgc agtaactaca ggaccaagtt ctcgtaaaag
     6301 cgagagtgaa accaaagttc ccaagctact ttcggcggca aaatcgacca ataccacata
     6361 gccttgtaag cctaacacca ttccgataaa taagcccgac agcataataa ttaagagcga
     6421 ttgcactcct aacacataaa gctgtttaat cagtaacggg gtatgttttc taaattcagg
     6481 tttaccgatt aatgctcccc ataacataaa agcggaacgc cctaatgtac ggataaaatt
     6541 aatcaccacc gccccgatag agctaataaa attaaccatt aaatagctcc tccgtataat
     6601 cttttgctgg ataatggaac cgcaccggac catcagcttt accggctaag aattgtacca
     6661 cttgcggatc ttcgcttgcc tgtaaaattt ccggtgtacc ttcagcaatc actcgtttat
     6721 ctgccacaat ataagcataa tcggcaatac ttaatacttc tttcacatcg tgagacacca
     6781 caatggaggt caaattcaat gcctgattta actctttaat cagctcgaca atcacaccca
     6841 tactaatcgg atcttgcccg gtaaacggct catcatacat aatcagatcc ggatctaacg
     6901 caatcgctcg agcaagggcg gcacgtcttg ccataccgcc ggaaagttcg gacggcatta
     6961 aatttgccgc acctcgcaac ccaaccgcct cgagcttcaa taacaccaat ttgcgaatta
     7021 gctcttcggg taaacgggta cgctcccgaa tcgggaaagc cacattatcg aaggtagaaa
     7081 tatcggtaaa taacgccccc gattgaaaca acatccccat tcgttggcga atttcataca
     7141 actctttgtt attggctttg caaatatctc tgccatcaaa taaaatttcg cccgattcag
     7201 gtaaaatctg cccaccgatt agcctcaaca gggtggtttt accaatccca gaagacccca
     7261 taatggcagt cactttacct ttctgcactt gcagattcag gttatcgtaa atcactcgct
     7321 cccctcgctt gaaggtcaag ttttttactt caaccaaatt ttccgttttt gtcatcaaat
     7381 aggactcaaa ttaataaaag tgatatttat ggtctatcaa atttgccaga atgtcaaaaa
     7441 acttttaata atctgacaaa aatctgcctt tacaagcggt cagatttgct aaattttttg
     7501 caaatcggct gaactgaaaa agaaaggtct tgtcttagcc aatacgagcg ttttcactcg
     7561 tagattataa aaacatagga tatgaggaac attcacaata tgaacaaaac aatgaaaaaa
     7621 tcatttttaa ctgcattcat tttaggctca accattgccg caatgccggc gtatgcagaa
     7681 gcaacattac ccacagccgt aaatgggcaa gctgtgccaa gccttgcacc aatgctggaa
     7741 aaagttcgcc cggcggtggt gagtattgca gtagaaggta aaaccaaagg cgatagccgc
     7801 cgcagtcgtg aaattccgga agaatttgaa tttttcttcg gtcctcacgc cgatttattc
     7861 ggcaaccgct catcagctcc acgcaatttc cgtggcgttg gatcaggtgc agtcatcaat
     7921 gccgaaaaag gttatgtgat taccaataac cacgtgatcg acaatgccga taaaattacc
     7981 gtaaaacttg aagacggacg tgaatttaaa gctaaattag ttggtgctga tcccctttca
     8041 gatgtggcgt taattcaagt tgaagatcca aaaaacttaa ctgaaattaa attcgccgat
     8101 tccgataaat tacaagtggg cgattttaca gttgcaatcg gtaacccgtt tggcttagga
     8161 caaactgtta cttcaggcat catctcggca cttggtcgtt caaccggtca atccgatgaa
     8221 ggctatgaaa actatattca aaccgatgcg gcagttaacc aaggtaactc tggcggcccg
     8281 ttaattaact taaacggcga attaatcgga attaacaccg ctattatttc tccaagtggt
     8341 ggcaatgccg gtatcgcctt tgcgattcca agtaatatgg caaacagctt ggtgactcaa
     8401 attattgagt ttggtgaagt aaaacgtgga atgttaggca ttaaaggcgg tgaattgaat
     8461 gctgaccttg ctaaagagtt caatatttcc acccaacaag gggcatttgt aagcgaagtc
     8521 ttaccggatt ctgctgctgc taaagccggc tttaaagcag gtgatgtgat tactgaaatc
     8581 aacggtcaaa aaatccgtag tttcagcgaa ttacgagcaa aagtagctac ttcaggtgta
     8641 ggtaaagaga ttgaacttac ttacctgcgt gacggcaaag aagccaaagc aaaagtgacc
     8701 ttacaatcgg attctgaagc caaagttact gcttcaaacc tcattccggc attaaaaggg
     8761 gcagatttta acaactacaa tgccaaaggc attaaagggg ttgaaatttc taacgtagaa
     8821 aaaggctcga ttgctgaaat gcgaagcctc aaaaaaggcg atattatcat cggcgtgaac
     8881 cgccactcgg tagaaaacat tgccgattta cgcaaaatat tagaaaccaa gccatctgtg
     8941 gttgcactca atattgtgcg tgacggtgca aatttctatg tgattttgca ataaaccttt
     9001 tgatccactc aaacaaaagc cgatgacaaa aatcatcggc ttttgttatt gctgggaatt
     9061 taacatctgt cgtgccattt ccatactttg gataatcgct ctgcggttga tagaatcatt
     9121 attgctatct aacactttac gccaattatc gagagcttgt tgaaaattgt tatttaaaaa
     9181 gctgtcggag gctagcagga gtaaactggc attttcattt ttatccaact gtagggcttg
     9241 ctcgataatg gcttgaattt caacggtcat tttctgcccg ttatcgtaat aacgagcggt
     9301 ggcaatcgcc cccaaaatag ccgctcgttt tccgagtagc ttctcggcat tatcgtagca
     9361 aaccagtgcc gaatcaaaat cattgcttaa cgcataagcc tgccctagtt cgtaccataa
     9421 atcaccgtta ttcgggtttt ctcgaagctg gttttgcaga ctcaaaatat agcgttcatt
     9481 tttcgatgtg ccgtgtgcat cgctcatctg ttgttgaaaa tcttgatgtt gttgcaaacc
     9541 tgattgcacc acttgataac gtccggaaag cccatagtaa gtcattgcta acaacaaaat
     9601 cgccacgcaa ctcaggcttg cgattttagc aagcggtcga ttttgagcat tattttgcaa
     9661 aacgctctgt tcaaattgcg ttcgttcaaa ttggtaacgt tgctctgcct cacgctcaaa
     9721 ctcttgtctg acgtcatctg caaaatgttg ggcaaattta accgcttgtt ggtagttttc
     9781 ttggcttaat tgctcacgcg ttttcatttt tgttgccttt atttttgcta gttcgccaca
     9841 aaatagctag aaataacagg ctaaacaagc caaccggcaa gccccataat acccatgtct
     9901 gtggcgtgaa tggtggatcg taattcacaa aactgccgaa acgctcggtc ataatgcgga
     9961 taatttcctc attacttttc ccttgattca ccatttcata cacttcaatt cgcatattgt
    10021 aagccgccgt cgcattggat tcgaccaaat tttggttctg acattgcaaa catcgcaacg
    10081 aacgagccag tgaaatcgcc cgtacccgat ccgcctcatt ttgaaattga aaggtgtcca
    10141 ccatttcagc ttgaaccgtg ccgacaaata gcaacaagcc aaatacaagc ggtaatattt
    10201 tgcggatttt ttgcaaaaag ccgatggaaa ttatgctatt tctcatttct gagagcctct
    10261 aatttgggta ggaaatcctg tgcaaaatca gggttgtaac ccttttgttg gtaacgcact
    10321 acaccgtgtt tatcaatcaa ataactggaa ggtgcagaag agactttcag ggtttggatc
    10381 aaaaagcctt tttcgtaatc atcaataatt aaactaaacg gatttcccca gtttttcaat
    10441 gcgttgaggg catcatttgg gcggtcgcta taagttaagc cgacaatcgg cacgcctttt
    10501 tgttttagct ccaacaaaat cgggaactct ttaatgcacc aagtacacca gctcgcccac
    10561 acattgagaa tataaggctc tttcggcaag ctgttgttat ttaaatgcgc gttactatca
    10621 agtaagttct tgcctacaaa ctctggaaac ggcttgccct gccaatcttc agtgggagca
    10681 agggcatctt tattcattaa cggcacagtc aaaaagccaa caataactac aagtaacagg
    10741 agcggaataa gtaacaggat ttttttcatt ttttgttttc tctacgataa ccgaataatg
    10801 ccaacagggc ggccattacc atcatcatac cacctaacca taaggcttgc acataaggct
    10861 tatagtgcaa acggaaggca tattctaaat tgccgaactt atcccccatc acaatataaa
    10921 tatcgtctaa attgtggtgg aaaagcccta cttctgccat tgtcatcgtt cttacatcat
    10981 aatagcgtct ttccggtgtg acggttgcca gcaactgttc atctcggctg atagaaaaaa
    11041 cggcttgctc ggcggtgtag ttcggaccga tgagatcgtg ataatcttca tatttaaagg
    11101 tataaccggc aagttccgct gcttgattcg gctttaaccg cacgccgatt tcatcgccgt
    11161 aataactgtt catcattgcc ccaatcacgc aaaccgcaaa gccgatatgg gctaaccgca
    11221 tcgcaagcgg tcgaatttgc cacatttttt gcgaataggg caaatgagtg agaataatcc
    11281 agatcgcaag gctgataaat atggtgggta acggctgcaa agcaaaatgc tctctttgcg
    11341 agatggaggc ataaatcaac cctaacgcta aaccgactgc aatcggcagc aaccaaagtt
    11401 ttaccaatat ctgcttattt gaaatctgtt tccagcccag caccaccgca aagcccatta
    11461 ctatcattaa cagtaacgct agtggcgtga atacgctatt gaaatagggg gcccccactg
    11521 aaatcgatcc ccaattcatc actgtgaaga tcatcgggta gaaagtcccc agcaatacca
    11581 cagaagcagc cacactaaat aagccgttaa tcaataaaaa tgccgtttct ttagaaagta
    11641 agttgaaacg cactttgcct tgccagagat tcgctcggaa tgcaaacaga gcaagggcaa
    11701 tgaaacttaa gccgaagaat aagatcagca gtgccattcc tctgtcggga tcgaccgcaa
    11761 aggcatgaac cgaggtcagt acacccgaac gaacaataaa cgtgcctaat aggcttaaag
    11821 caaaggcgaa aatggcaagt agaatcgtcc aataatagaa aatgccacgc tgttggctca
    11881 caattaaact atgcaccaat gcggtgccaa gtagccaagg cattagggag gcattttcca
    11941 ccggatccca aaaccaccag ccaccccagc cgagttcgta atatgcccac cacgccccta
    12001 aaataatgcc ggcggtgaga aatccccacg aaatcatcgt ccacggacga agccaacgca
    12061 taatcgcagc gtccattccg cctgaaacga gggcggcgac aatcattgca aagctcaccg
    12121 caaagccgac atagcccaag tagagcaaag gcgggtgaaa gatgagccca atatcttgca
    12181 acattgggtt gagatctcgc ccttcaggtg gtggtggaaa gccacgttca aatgggctgg
    12241 aaaccaatag aatgaaaagt agaaaaccta gtgcaattag ccctaacatc gctaaggttc
    12301 ggttggcaaa caagcggtca attttttgcg aaaatttgca aaataccgca gaccaaatca
    12361 caagagcggt gagccaaaac agcattgaac cttcgtgtcc gccccaagtg gcggcaaatt
    12421 taaaaaaatc ggggagctgg ctgttggaat gagcggcaac gtagataacc gaaaaatcat
    12481 cggttagaaa agcataagct aaggtagaaa aggaaaatgt ggtaaaagca gcttgcaaat
    12541 aagtgaaaag cggagccaat gccagccaat gatattgctt gcgaacttcg cctagaagtg
    12601 caaaaccaac ttgaaaaatg gcactaattg cagccaataa aagtgaaaaa tagccgagtt
    12661 ccggtagcat aaaaaatcca aaaaatgacc gcttgtgaat gtaaaaaaag ccctctccac
    12721 ttgtgggaga gggacagatt tttcttcgtt cagaagaaaa atcagggaga ggggaattaa
    12781 ataattttgc taaaaataac aatactccct ctctctgaaa aatatttctg aacgaaatat
    12841 ttttcggtct caatactcgc tacgctcgct ccttcggagc cgttggcaaa gccaacgttc
    12901 aaatgtaaac atttgtccca caaggggaga gagtatatcc taacttgcaa ccgttagttg
    12961 ccctgcataa aggataaagt aacgcaagca gagtacaccg attaagtcga aaacggaaat
    13021 taagataata aatttcacgt tatgtttcaa gcggtggcta ccaaaaatat tggcaacaag
    13081 cggaatcgca ataccgatta acattacgcc tatccagaac acattccccc agaagccgtt
    13141 aagtgcattg gttaaagcga gcgatttatc tgctccaccg aagtgtaagc ccacaaagaa
    13201 cgctacaatt aagcctaatt cggttaccat aatcggcact tcaaatttgt gcataaagtg
    13261 gacttcgtgg ctttcaccgg ataacttgcc tgcgagtaaa atcactagga aagttgctgc
    13321 aatacctgac gatgcccctg atgccaagaa caatgccggt aacaccgggt tgttgagcat
    13381 tggataactc actaacgccg agagtaagaa accggtgtaa gcaccaagta cagcagccaa
    13441 tacgaataag ataaattcaa ttggttttaa cagcttcgct tcggcaaaat cgatcaaacc
    13501 agagaccaca ggtagatacc gaacaatcca atctttaaac attatggcga tccaaaccac
    13561 caatactgcc atataaagtt ggaataacat tacccccatc gacattactg aggtaaagtt
    13621 atagttgaac attaatttcc agaatgtcca aggtttggtt aagtggaaaa tcagtaacag
    13681 taagccgata atggttggta atgtgccaag aatagccgca ctacgaataa tccagttttg
    13741 gcttggtttt tccattttga caccgctatt acggtaagca atcgctaact gaaccgcacc
    13801 tgatgaaata ccaagtagga ataagtaaat agcgatagtg ccatcccaaa caaggtttgg
    13861 ggtttggaaa ggtacatatt cattcattat ctatgttctc ctttaccaaa tggaatgtga
    13921 tataaattcg gctccgtgcc taatgccgtt ttggtgcgat aaaccggatt atttttcact
    13981 ttatggaaaa tatcgctact tggatcattc atatcaccaa aggttaaagc tttagtcgga
    14041 caagcctcta cacaagcagg ctgtttgcct tctgcaagat tggtatctcg gcaaaagtta
    14101 catttatccg ccgatttttt ctcagggtga ataaaacgta cacgatacgg gcaaaccgca
    14161 atacagtatt ggcaaccgac acacaaatct gagtgtacat caacaatccc ggtttcagga
    14221 tcgataaatg aagcaccggt cgggcaaaca ctcacacaag gtgcattggt acagtgttgg
    14281 caagattggc ggaaaaactc atattgtacg ttcggaaact caccgaaagg ctcactacgg
    14341 ataatttcta aacgagaaac cccttccggc actttgttgg tggtgcgaca agcgtccata
    14401 caagccgtac agccgataca ggcagtttca tcgtgaacca tcgcatagcg gattttcttt
    14461 tgttctttct cagtagcaag agttatggag gtggcaagcc ctgtacttgc cacaactgcg
    14521 atagcacctg cccctgagac gaaatctcta cgggtgcaag tcattatttt gttccttttg
    14581 cttctgtggc tttcatttca ggatttttaa attcgcctga atgaattttg gtatgacaat
    14641 ccgtacaaag tttaatgcga tctttttcag gaatgtcttt catcggatct ttttccgggt
    14701 gaatttcatg acagttggta caagcaattt tagttgcgtg cgtgtcgtga gcccagaatt
    14761 tttcacgcaa tttctctgcc gtatggcaag caaaacagac ttgattttgc tcatttaccg
    14821 aacgttcaag tgatgggtta cttgctttac cgtgtgggtt aaaacgcatt acgtcttttg
    14881 cccctttgcg gtggctttca gaaatattac cgtggcaact tacacaagtg agtggctctc
    14941 cattattcgg gcttttcaca tctttttgga aatgtttgcc tgcgtgatgg aaaggcttac
    15001 ctgttgcgtt tgccgattcg tgacattgag tacaatattt atttggatca cggcgtgcat
    15061 cttgagctac ttttgcatca tcagccgttt ctgattgcca aagaggtttt ggctgctcag
    15121 gcattaacgc attcgcactg gaaattgcaa aaaaacctgc aaatacgacc gcttgtatga
    15181 gggcgactgc tcgtcctgct tttgagatta attttttcat agttcacctc aagctacatt
    15241 attttgcttc tgcttttggt gctgctaccg gtttgtctgc ttcttctgcg ggtaataaac
    15301 ctttagcacg agcttctttt tcccattgag gaaccacagt ttttaagaac tcatcttttg
    15361 ctgcttgctc tttaggaata tctaaaccaa tagcaagttg tgctttctct ttagttgaga
    15421 tgtccggaat cgccactggc gttgttacac cgtgtttcgc taacactaca cctaatttag
    15481 cacgggcatc tgctgcacgg tcgatacctg ttgcgatggt acgtaacatt acatcaggtg
    15541 cgtggaagtg gttaccgtga cctgccgcag agtaatccca acgccattga gcgtgacgaa
    15601 ttgccattaa gatctctttc atctcttctt gagttgctcc agcttcccaa gcagctttcg
    15661 cttcaaagtg agctttcact aattgatctt ctaagcgaat cattgcctct tttacttcgt
    15721 gtttatactc ttttacacgt ttttccaatg tttctttgct ttgttcatga caagttttac
    15781 aagtcgcttc aaattggtca aacggattac cgattttgtg gtcagtataa actttgccgt
    15841 ctttgtcttt cacttttggc atatggcaat caatacaggt tacaccattt ttcccatgtg
    15901 taccttgtga ccaaacttca aaatctgggt gctgcgtttt tagcatcgga gctttcgata
    15961 aagcatgagt ccagtctgta aaattgattt catcgtagta tttttcaatg ctatctacat
    16021 ctacaccttt atcccaaggg aaagttacat ctgttttatt tttgaaatag tactccacat
    16081 ggcagttagc gcacacagca gggcgtttac catgtttatc taaatcctca aacttccaac
    16141 caaccgtatt caatgcacgt aatacgtgag gacgtgcaac acgtagtgca ggtttacctt
    16201 gtttaaactc ttctgatgtc gtgtcatggc agtctgcaca acctattgag tttacaattt
    16261 cagcaccata tttcgcccat ttaggatcaa aatatcctcg ctcacctttc tcctcaatta
    16321 agcgtggaac atcagggcct ttacatgtcc aacaagccat aggttgtgga ccatcatttt
    16381 catctttagg cgcgccggta cgcaaaatat cacgcacatc ggtaactgca taaaagtgac
    16441 ctcgtggttt attataatct ttcgcaaaag catagcctgc ccacaaaata acataacgag
    16501 ggtcagcctc taacgcactt cctcgatctg tggattttgc agttgacttc caagaatcgt
    16561 attgtagcgg atatttttct gcaaataggt ggtttgcaga ctcaattttc acgcctaatt
    16621 tttcatgctt aactgcttta tctgttaatg attcagtttt cgcctcttca gctaaagcag
    16681 tttgtaaaca ccccaatatt gaaattgttg ccacagctag gctttttatt aaaaatttca
    16741 ttttggctct cccactcagg taaaaataat tagataaaaa taagaatttt tcctattaaa
    16801 attaataaga taatccctga tattcagttg cgagaatatg aaatttaaac caagccgact
    16861 atatcaaaat gaagatttat tgaattaaa
//
LOCUS       DANJWC010000039        16761 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.039, whole genome shotgun sequence.
ACCESSION   DANJWC010000039 DANJWC010000000
VERSION     DANJWC010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16761
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.039"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            224..3295
                     /gene="fdnG"
                     /locus_tag="PWX02_000935"
     CDS             224..3295
                     /gene="fdnG"
                     /locus_tag="PWX02_000935"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014550636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:809..811,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDL1728964.1"
                     /translation="MQISRRKFFKVCAGGMAGTSAAMLGFAPATALAAPREYKLLRAK
                     ETRQTCTYCAVGCGMIMYSLGGEAMNSKGKLIHIEGDSDHPVSRGALCPKGAGALDYV
                     NSDRRVHYPEVREAGSSEWKRISWHEAIERIARHMKNDRDENFVTHNEAGLEVNRWMT
                     AGFLAGSACSNETGIVTQKFIRSLGMIFTDNQASIUHGPTVASLAPSFGRGAMTNHWV
                     DIKNSDLVIVMGGNAAEAHPVGFRWAVEAKKQNGAKLMVVDPRFNRSAAVADIYIPLR
                     PGTDIAFLLGAVNYLLKNDKIQHEYVKHYTNATFLLNEGFKFEDGLFTGYDETTRTYK
                     DKSTWAYQLDEQGQPKRDMTMQDPRCVINMLKDHVSRYTPEMVERITGTPQKDFLIFC
                     EEIAKTSAPDKAATFLYALGWTQHTVGAQNIRSMAIIQLLCGNIGVSGGGVNALRGHS
                     NVQGITDLGLFPHMLPGYIRLPTEADATLESYLNAIAPKTLVAGQVNYGQNNPKFAVS
                     MLKTFYGDKATKENEFGYHYLPKYPKGGTDHFRFIEQMYQGKVNGYFCQGMNPIASYP
                     NSQKLIKALSNLKYLVIFDPLITDTSEFWKNHGEFNNVNPSEIQTEVFRLPTTCFAEE
                     DGSIANSGRWLQWHWKAADAPHEAKPDVDILSEIRAELIHLYHKEGGKAPLEPLEAMT
                     WNYMNPLEPKPEEIAKENNGYALEDLKDADGNIIVKKGELLSSFAQMRDDGTTSGGCW
                     IYCGQWTPKGNQMANRDNSDPSGLGNTLGWAFSWPVNRRILYNRASADLSGKPWNPKR
                     QLVKWNGKNWNYVDVADYGTAPPNSPVTPFIMQPEGVSGLFVRERMQDGPFPEHYEPF
                     ETPIGTNPLHPNVISSPVARILPSDKADLGTSDEFPYVGTTYRLTEHFHYWTKNALIN
                     VIAQPEQFVEIGEKLAEEKGIKNGDKVRVWSKRGEIKAVAVVTKRIRSLVSDGKPIHT
                     IGVPIHWGFAATTGAKKGYFANNLTVRTGEPNSNTPESKCMLLNIEKIVGA"
     gene            3298..4212
                     /gene="fdxH"
                     /locus_tag="PWX02_000936"
     CDS             3298..4212
                     /gene="fdxH"
                     /locus_tag="PWX02_000936"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HDL1728965.1"
                     /translation="MSTVQTQNIIKISATSALTPAPQARSHQVEVAKLIDVTTCIGCK
                     ACQVGCSEWNDIRSDVNANCVGVYDNPADLNAKAWTVMRFNEVEENDRLEWLIRKDGC
                     MHCAEPGCLKSCPSPGAIIQYANGIVDFQSDKCIGCGYCIAGCPFNIPRMNPDDNRVY
                     KCTLCVDRVNVGQEPACVKTCPTGAIRFGSKEEMKFYAEQRIADLKSRGYENAGLYDP
                     EGVGGTHVMYILHHADKPELYSGLPKDPKIDTTVTLWKDVLKPVAAVALGGLALAEVA
                     HYITVGPNVEEDLEEHHHSENAEGGKHE"
     gene            4205..4882
                     /locus_tag="PWX02_000937"
     CDS             4205..4882
                     /locus_tag="PWX02_000937"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HDL1728966.1"
                     /translation="MSKKFEITNDTKIVRHKPLARVSHWFLVISFFMTMFTGVAFFFP
                     DFAWLTEILGTPQIARAVHPFTGIIMFIAFIIMALIYWHHNIPEKNDIRWAKGIVEVL
                     KGNEHKVSDNGKYNLGQKLLFWTLILAMFTLLATGIIMWRQFFSHYFSIPMLRIAILL
                     HSASAFMLFTGILVHMYMAFWVKGSIRGMVEGWVTVRWAKKHHPRWYREEVLPELERQ
                     VAEQNNK"
     gene            4938..5855
                     /gene="fdhE"
                     /locus_tag="PWX02_000938"
     CDS             4938..5855
                     /gene="fdhE"
                     /locus_tag="PWX02_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HDL1728967.1"
                     /translation="MSIKILPQDDIKQAASSFQQPELLFANPKNLYLRRAKRLRELAK
                     QNPFGEYMEFSANLVDIQLALLESQPIADYSQKITACVERTQGETPLNAQNFQRTDEW
                     RTLLLAIIEKFKPYANDTVLATIEVLEKAAKSELDTLADNLLNERYELVGADKAVFIW
                     AALSLYWVQLAQQLPRNSRAEIGHKHLCPVCNSAPVSSVIHFGEAQGLRYLHCSLCES
                     EWHVVRTKCTNCDETGKLDYWSLDTVEAAVKAESCGDCHSYLKVLYQDKDVNVEPVAD
                     DLATLFLDSEMEQKGLSRSGINPFLFQVE"
     gene            complement(5925..6311)
                     /locus_tag="PWX02_000939"
     CDS             complement(5925..6311)
                     /locus_tag="PWX02_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728968.1"
                     /translation="MLRNFMIMLHFIYGFAMKNAFSFFVISLFLTMNSISYASNPIPS
                     NKEKLNGVKKQQDKPKFRISERKVLKNKEKDEEVSNFSMEELRVGAQRPLDDSVIQYF
                     KSQCRYAFMSDREIIANRCEVKKIAD"
     gene            complement(6322..7692)
                     /gene="gorA"
                     /locus_tag="PWX02_000940"
     CDS             complement(6322..7692)
                     /gene="gorA"
                     /locus_tag="PWX02_000940"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDL1728969.1"
                     /translation="MAKHYDYIAIGGGSGGIASINRAASYGQKCAIIEAKHLGGTCVN
                     VGCVPKKVMFYGAQVAEAINHYAPDYGFDITVNNFDYAKLVESRQAYIGRIHTSYGNV
                     LAKNNVDVLNGFARFKDAKTLEVSYADGSVEEVTADHILIATGGRPTVPNVKGAEYGI
                     TSDGVFALDALPKSVAIVGAGYIAVELAGVFNSLGVNTHLFVRQHAPMRNQDPLIVDT
                     LLEVLAQDGIELHTQATPQEVVKNADGSLTLKLQDGRETSVESLVWAIGREPATDKIN
                     LEAAGVETNERGFIKVDKFQNTNVPNIYAVGDIIEGGIELTPVAVAAGRRLSERLFNN
                     KPNEHLDYSLVPTVVFSHPPIGTVGLSEPKAIEQYGAENVKVYKSSFTAMYTAVTQHR
                     QPCRMKLVCVGEEEKVVGLHGIGFGVDEMIQGFAVAIKMGATKADFDNTVAIHPTGSE
                     EFVTMR"
     gene            complement(7769..8293)
                     /locus_tag="PWX02_000941"
     CDS             complement(7769..8293)
                     /locus_tag="PWX02_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HDL1728970.1"
                     /translation="MHSELNTRANILGKIIFASRWLQLPIYLGLIVVQGIYAYKFIKS
                     LYHLVINLNEMDSNTVMLAVLNLIDVVMIANLLVMVIVGGYETFVSRLKVDDHPDQPE
                     WLDHVNATILKVKLSMAIISISSIHLLQTFINASNLDEKQMMWQVVIHLAFIVSALAM
                     AYIDKMLNSGHMKH"
     gene            complement(8388..9026)
                     /locus_tag="PWX02_000942"
     CDS             complement(8388..9026)
                     /locus_tag="PWX02_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL1728971.1"
                     /translation="MELKHTIEQRKTIKAFNSTIKISRTELEEMVALSQLAPSKANLQ
                     PWRFVIIDDEILKAALSKNVAFNGPPCETASAIVVILADLQYEKLLGAIVDQSVETGC
                     LHKEFRERKFDFLLNLHNQATQAEIRDQVLIDTSLAAMQLMLIAKDKGYDTHAIGVFE
                     RPAVLKLLNIDAERYLPVMLLAIGKAANPALPSCRLPLEYTVSWNDGQGFKI"
     gene            complement(9168..9962)
                     /locus_tag="PWX02_000943"
     CDS             complement(9168..9962)
                     /locus_tag="PWX02_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1728972.1"
                     /translation="MALLEVNHLNKRFVERSSLFHKQDFYAVKEVSFTLNRKETLAII
                     GENGAGKSTLAKMVAGMVEPTSGELIFRGTPLSFGDYKYRAKHIRMMFQDPNDAFDPN
                     YNIGQILDAPLRLATDLNEEERNERIFNTLKLVGMYPEQALVPISEASSGQKQRIAFA
                     RALILEPEIIIADDTINSLDFSVKTQLINLMLSVQQRQGIAYIYVGQNLGLIKHIADK
                     IMVMQSGEVVEYGLTKEVLLNPQHQITKRLIESHFGKHLSSYAWAS"
     gene            complement(10063..11139)
                     /gene="fbaA"
                     /locus_tag="PWX02_000944"
     CDS             complement(10063..11139)
                     /gene="fbaA"
                     /locus_tag="PWX02_000944"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HDL1728973.1"
                     /translation="MSLLNIVKPGVVTGDDVQKVFAYAKEHNFAIPAVNCVGSDSVNA
                     VLETAARVKSPVIVQFSNGGAQFYAGKGIKPASGARADVLGAIAGAKHVHQLAEEYGV
                     PVILHTDHAAKNLLPWIDGLLEAGEKHFAETGRPLFSSHMIDLSEEPIEENMEICREY
                     LARMDKIGMTLEIEIGVTGGEEDGVDNSDVDESKLYTQPSEVLYVYDQLSPISPRFTI
                     AAAFGNVHGVYKPGNVKLKPSILGASQEYVSKERGLPAKSLDFVFHGGSGSSREEIRE
                     AISYGAIKMNIDTDTQWAAWDGILQFYKANEAYLQGQLGNPTGPDAPNKKYYDPRVWL
                     RKMEESMSKRLEQSFEDLNCVNVL"
     gene            complement(11236..12411)
                     /locus_tag="PWX02_000945"
     CDS             complement(11236..12411)
                     /locus_tag="PWX02_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDL1728974.1"
                     /translation="MSVIKMTDLDLAGKRLFIRADLNVPVKDGKVTSDARIVATIPTL
                     KLALQKGAKVMVTSHLGRPTEGVFEEENSLQPVVDYLNASDLGVPVRLVRDYLDGVEV
                     NENEIVVLENVRINKGEKKNDPELAKKYASLCDVFVMDAFGTAHRAEGSTYGVAEYAP
                     VACAGPLLAAELDALGKALKEPQRPMLAIVGGSKVSTKLTVLDSLSKIADQLIVGGGI
                     ANTFIAAEGHAVGKSLYEEDLIPEAKRLAQVTNIPVPVDVRVGTEFSETAPATEKAVS
                     EVRADESIFDIGEKSAEQLAEIIRNSKTILWNGPVGVFEFPNFRKGTEVISNAIAEAT
                     ANGAFSIAGGGDTLAAIDLFGIKDKISYISTGGGAFLEFVEGKVLPAVEILEKRANG"
     gene            complement(12667..14025)
                     /locus_tag="PWX02_000946"
     CDS             complement(12667..14025)
                     /locus_tag="PWX02_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HDL1728975.1"
                     /translation="MANQQQERQSWSNRLAYIMTVAGATVGFGATWRFPYLVGEHGGG
                     AYVFLFCIAMIVIGIPMILVENVIGRRMRVNAIDAFAGFANGKKIASGWKILGYMGLL
                     GAFGILAYYMVLGGWVLNYIGGLLTGTLDLSQPVTVGTTQQFFQQSIFNSPMTIIAYT
                     ALFVLVNYFILVKGIVDGIERSVKYLMPILFIFLIVMVVRNITLPGAAEGIAFYLMPD
                     FSKITPKLFVFVLGQVFFALSLGFGVLITLSSYLDKSENLVKTATITGVMNTLIAVLA
                     GFMIFPSLFSFGVAPNSGPTLVFQSLPIVFSNMWGGTVFAVIFFSLLVVAALTTSLTI
                     YEVIITALQEKAKISRQKAIFITLATIFLLGNIPSALSDNILQDVQVMGKSIFDAFDY
                     ISGNILFILTALGSAIFVGFVLKEEAKEELGAGKMFTTIWFNYVRFVIPVIILVIFFN
                     SL"
     gene            complement(14095..14922)
                     /locus_tag="PWX02_000947"
     CDS             complement(14095..14922)
                     /locus_tag="PWX02_000947"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HDL1728976.1"
                     /translation="MNHPKHRAFLKWAGGKYRLIPDIQTHLPKKACLVEPFVGAGSVF
                     LNTDFERYILADINPDLINLFNVVKADVEEYIAQTKQLFLHPQANTEAFYKACRAEFN
                     RSKDIFRRSVIFLYLNRFGYNGLCRYNQKKGYNVPFGRYKTHYFPEKELRFFAEKAQK
                     ATFVCADFEQVFELAKNQLTDYVIYCDPPYAPLAQETNFTQYAGGGFSLEQQGRLATL
                     ALEMKGLGVPVLISNHDTDFTRRIYQSADITQIQVQRSIGQKVGSRVKVGELFALFI"
     gene            complement(14936..16024)
                     /gene="aroB"
                     /locus_tag="PWX02_000948"
     CDS             complement(14936..16024)
                     /gene="aroB"
                     /locus_tag="PWX02_000948"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDL1728977.1"
                     /translation="MLQVNVELKERRYPIIIGAGLLNQPASYSPLKSGDKVMIVSNPT
                     VATHYLSVVTNALKELGCHVDSVLIPDGEEYKTLESLNLIFTALLEKNHNRDTTLIAL
                     GGGVIGDVAGYAAASYQRGIRFIQIPTTLLAQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     VSVIVDTNTLHTLSKREVSAGLAEVIKYGAIFDVTFFEWLEKHIDDLVSLKQDELEYC
                     IQRCCQLKADVVARDETEKGDRALLNLGHTFGHAIEARMGYGVWLHGEAVSVGMLEAA
                     ELSRILGDLSPQEVARLEKLLARANLPTISPDGMEPAEYLPYMWRDKKVLAGQLRLIL
                     LKSLGEAYVSSQATESQVLASIERFTQR"
     gene            complement(16048..16569)
                     /gene="aroK"
                     /locus_tag="PWX02_000949"
     CDS             complement(16048..16569)
                     /gene="aroK"
                     /locus_tag="PWX02_000949"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HDL1728978.1"
                     /translation="MAEKRNIFLIGPMGAGKSTIGRQLAQTLGMEFMDSDSVIEERAG
                     ADIDWIFDVEGEEGFRKREERILNELTQNYGIVLSTGGGSILSKDNRNVLSARGIVIY
                     LETTVDKQFERTQRDKKRPLLQTEDPRKTLEELAKIRNPLYEEIADITIHTDEQSAKV
                     VANQIIDMIDNLQ"
BASE COUNT         5179 a         3641 c         3127 g         4814 t
ORIGIN      
        1 tcacttgact aaaaagtagt ttattttgtt aaaaagttgt atcattaaat tgtttaaatt
       61 tggtttacat caaattaatt taagatttta tctatttacc aaaaaaagag tatgttcatt
      121 ttcttttatt agggggaaaa tgatacacct cttagatcaa tttattaaat taattttgat
      181 caaattttat tttatttctc acattatgtg atttggagtg attatgcaga tctcaagacg
      241 taagttcttc aaggtctgtg caggtggtat ggcgggaact tctgctgcaa tgttgggttt
      301 tgctccagca acagcattag ctgcaccgcg tgagtataag ctcttaagag caaaagaaac
      361 tcgacaaacc tgtacttact gcgcggtagg ctgtggcatg attatgtaca gcttaggcgg
      421 cgaagcaatg aacagcaaag gaaagttaat tcatattgag ggtgattctg accatccagt
      481 aagccgtggg gcgttatgtc cgaaaggtgc aggggcgttg gactatgtta atagtgaccg
      541 ccgtgtgcat tatccggaag tgcgtgaagc cggttcaagt gagtggaaac gcatttcttg
      601 gcacgaggca attgaacgta ttgcccgcca tatgaaaaat gaccgtgatg agaacttcgt
      661 cacccacaat gaagcagggc tggaagtaaa ccgttggatg acggcaggtt tcttggcagg
      721 ctcggcgtgt agtaacgaaa ccggtatcgt cacccagaag tttatccgct ctctcggtat
      781 gatttttacc gacaaccaag cgagtatctg acacggacca acggtagcaa gtcttgctcc
      841 atcatttggt cgcggtgcga tgaccaacca ctgggttgat attaaaaact ccgatttagt
      901 gatcgttatg ggcggtaacg cagccgaagc tcaccctgtg ggtttccgtt gggcagttga
      961 agcgaaaaaa cagaacggtg cgaagttaat ggtggtagat ccacgcttta accgtagtgc
     1021 tgcggtggcg gatatttata taccattgcg tccgggtacg gacattgcgt tcttgttagg
     1081 tgcggtaaac tacctgctta aaaacgacaa aattcaacac gagtatgtga agcattacac
     1141 caatgccaca ttcttactca acgaagggtt taaattcgaa gacgggcttt tcaccggcta
     1201 cgatgaaacc actcgtacct ataaagacaa atccacttgg gcttatcagt tagatgaaca
     1261 gggtcagccg aaacgggata tgacaatgca agatccacgt tgtgtgatca atatgctcaa
     1321 agaccacgtt tcccgctata cgcctgaaat ggtagaacgc attaccggta cgccacaaaa
     1381 agatttcctg attttctgtg aagagattgc gaaaacctca gcgccggata aagccgccac
     1441 cttcctctat gccttaggtt ggacacaaca caccgtgggc gcacaaaaca ttcgctcaat
     1501 ggcgatcatt cagttgctgt gcggtaacat tggtgtctcc ggcggtgggg taaacgcact
     1561 gcgtggtcac tctaacgtac aagggattac cgacctaggc ttgttcccac atatgttgcc
     1621 gggctatatc cgcctgccaa ccgaagcgga cgcaacctta gaaagctact taaatgcgat
     1681 tgcaccgaaa accttagtgg cagggcaagt aaactacggg caaaacaacc cgaaattcgc
     1741 tgtgagtatg ctcaaaacct tctacggcga taaggcaacc aaagagaacg agttcggcta
     1801 tcactattta ccgaaatacc cgaaaggcgg tacagatcac ttccgcttta tcgaacagat
     1861 gtatcaaggc aaagtgaacg gctatttctg ccaaggtatg aacccgattg cctcttaccc
     1921 taactcgcag aagttaatta aagcgttgag caatttgaaa tatttagtca tttttgaccc
     1981 gcttattacc gatacttccg agttctggaa aaatcacggt gagttcaaca atgtgaaccc
     2041 gtctgaaatt caaaccgaag tgttccgctt gccgaccact tgctttgcgg aagaagatgg
     2101 ttcaattgcg aactccggtc gctggttaca atggcactgg aaagcagcag acgcaccaca
     2161 cgaggcaaaa ccggacgtgg atattctgtc tgaaatccgt gccgaactga tccaccttta
     2221 tcataaagaa ggcggaaaag caccgcttga accgttagaa gcgatgacgt ggaactatat
     2281 gaacccgtta gaaccgaagc cggaagagat cgccaaagag aacaacggtt atgcattgga
     2341 agatctgaaa gatgcagacg gcaacatcat cgtgaaaaaa ggcgaactat tatccagctt
     2401 tgcacaaatg cgtgatgacg gtacaacctc cggtggttgt tggatctact gtggtcaatg
     2461 gactccgaaa ggtaaccaaa tggcgaaccg tgataactcc gacccatcag gcttaggtaa
     2521 caccttaggc tgggcattct cgtggccggt aaaccgccgt attctataca accgtgcatc
     2581 ggcagattta agcggtaaac cgtggaaccc gaaacgccag ctagttaaat ggaacggtaa
     2641 aaactggaac tatgtggacg ttgccgatta cggtacagca ccaccaaata gccctgtgac
     2701 accatttatt atgcaaccgg aaggggtatc cggcttgttc gtgcgtgaac gtatgcaaga
     2761 cggtccattc cctgagcatt atgaaccgtt tgaaacgcca atcggcacga acccgttaca
     2821 cccgaatgta atcagcagtc cggtggcacg cattctgccg agcgataaag cggatttagg
     2881 caccagcgat gagttcccat atgtcggtac aacttaccgc ttaaccgaac acttccacta
     2941 ctggacgaaa aatgccctga ttaacgtcat tgctcaaccg gagcaattcg tggaaatcgg
     3001 cgagaaactt gccgaagaga aaggcattaa aaatggcgac aaagtacgtg tttggtctaa
     3061 acgtggcgaa attaaagccg ttgccgtggt gactaaacgt atccgttcat tggtctctga
     3121 cggcaaacca atccacacta tcggtgtgcc gatccactgg ggctttgcgg ctacaaccgg
     3181 tgcgaaaaaa ggctattttg ccaataactt aaccgtgcga acaggtgaac caaactcaaa
     3241 tacacctgaa tcgaaatgta tgttattaaa cattgagaaa attgtggggg cttaacaatg
     3301 agtacagttc aaactcaaaa cattattaaa atctccgcaa cctcggcgct gacaccggcg
     3361 ccgcaggctc gttcgcacca agtggaagta gcgaaactga tcgatgtcac cacttgtatc
     3421 ggctgtaaag cctgtcaagt gggctgttca gaatggaatg acattcgctc cgatgtaaat
     3481 gcaaactgtg tcggcgtgta cgacaaccct gcggatctaa atgcgaaagc gtggacagta
     3541 atgcgcttta acgaagtaga agaaaacgac cgtttagaat ggttgatccg taaagacggc
     3601 tgtatgcact gtgccgagcc gggttgcttg aaatcttgcc cgtcaccggg tgcgattatc
     3661 caatacgcca acggtattgt tgatttccaa tccgacaaat gtatcggttg tggctactgt
     3721 atcgcaggtt gtccgttcaa cattcctcgt atgaatccgg acgataaccg tgtttacaaa
     3781 tgcaccctgt gtgtggatcg tgtcaatgtt ggtcaagaac cggcttgcgt gaaaacctgc
     3841 ccgaccggtg caatccgctt cggtagcaaa gaagagatga agttctatgc agaacaacgc
     3901 attgctgatc tgaaatctcg tggctatgaa aatgccggct tgtacgaccc tgaaggcgtt
     3961 ggcggtacgc acgtaatgta tatattacat catgccgaca aaccggagct ttattcaggc
     4021 ttaccgaaag atccgaagat tgataccaca gtaaccttat ggaaagacgt tttaaaacct
     4081 gtggcggcag tagcgttggg tggtttagct ttagcagaag ttgcacacta tattaccgtt
     4141 gggccaaacg ttgaggaaga tcttgaagaa caccaccata gcgaaaatgc agaaggaggc
     4201 aaacatgagt aagaaatttg aaatcacgaa cgataccaaa atcgttcgtc ataagccctt
     4261 agctcgtgtt agccactggt tcttagtaat ctcgtttttt atgacgatgt ttaccggtgt
     4321 tgccttcttc ttccctgact ttgcttggct taccgagatt ttaggtactc cacagattgc
     4381 ccgtgcagtc cacccattta ccgggattat aatgtttatt gcatttatta ttatggcgtt
     4441 aatatattgg caccataata tccctgagaa aaatgatatt cgttgggcaa aaggcattgt
     4501 tgaagtacta aaaggtaatg agcataaggt ttccgataat ggaaaataca atttgggtca
     4561 aaaattactc ttttggactc ttattcttgc gatgttcact cttttagcca cagggattat
     4621 tatgtggaga caatttttct ctcactactt ctcgattcct atgctaagaa ttgcaatttt
     4681 acttcattca gcaagtgcat ttatgctatt taccggaatt ttagtacata tgtatatggc
     4741 attctgggta aaaggctcaa tcagaggtat ggttgagggt tgggttaccg ttcgctgggc
     4801 aaaaaaacac cacccaagat ggtaccgtga agaagtgcta cctgaattag aaagacaagt
     4861 agctgagcaa aataataaat aacctcaagt gcggtattat taccgcactt ttattttatt
     4921 ggtttaaaaa gaatattatg agtatcaaaa tactacctca agatgatatc aaacaagcag
     4981 ctagttcatt tcagcaaccc gagctcttat ttgctaatcc caaaaatctg tatttacgcc
     5041 gcgctaagcg gctacgtgaa ttagccaagc aaaacccatt cggtgaatat atggagtttt
     5101 ctgctaacct cgtggacatt caattagcat tacttgaaag ccagccaatt gcagattatt
     5161 ctcaaaaaat aaccgcttgt gtagagcgca cgcaaggaga aacaccgcta aatgcacaga
     5221 attttcagcg tactgatgaa tggcggacac tactcttagc cattattgag aaattcaagc
     5281 cttatgcaaa tgatactgtt cttgcaacaa ttgaagtatt ggaaaaggca gctaagtcag
     5341 agctagacac acttgctgat aatttgctaa atgaacgata cgaacttgtg ggcgcagata
     5401 aagctgtctt tatttgggct gctctctcac tttactgggt acaattagct caacaattac
     5461 cacgtaattc tcgtgcagaa ataggccata aacatttatg tccggtatgt aactcagcac
     5521 ctgtaagcag tgtgattcat ttcggtgaag ctcaagggtt acgttatcta cactgttctt
     5581 tatgtgaaag tgaatggcat gtagtacgta caaagtgtac aaactgtgat gaaaccggca
     5641 aactggatta ttggagctta gatacagtag aagcagcagt taaagctgaa agttgtggcg
     5701 actgccattc ctacttaaaa gtactctatc aagataaaga cgtgaatgtg gaacctgttg
     5761 ctgatgattt agcaactcta ttcttagatt ccgagatgga acaaaaaggt ctttcaagaa
     5821 gtgggataaa tccattttta tttcaagtag agtaactcat ttacaacaaa aacggagcat
     5881 aacgctccgt tttttattac atttaactca tacactatac ttttttaatc agctattttt
     5941 ttaacttcac agcggttagc aataatctcc cgatctgaca taaatgcgta tcggcattga
     6001 gatttaaaat attgaatcac agaatcatca agaggtcgtt gtgctccaac acgtaattcc
     6061 tccatagaaa aattactaac ctcttcatct ttctctttat ttttcaatac cttacgttca
     6121 gatattctaa acttaggctt gtcttgctgt ttttttactc cgtttaattt ttctttatta
     6181 ctaggaatag gattacttgc ataactaata ctattcatcg taagaaaaag agaaataaca
     6241 aaaaagctaa aagcgttttt catagcaaac ccataaataa aatgtagcat aatcataaaa
     6301 ttacgtaaca ttttattaca tttagcgcat ggttacaaat tcttcagatc ctgtcgggtg
     6361 aatagccact gtgttgtcaa aatcagcttt cgttgcgccc attttaattg caacagcaaa
     6421 accttgaatc atttcatcaa caccaaaacc aataccatgt aacccaacca ctttttcctc
     6481 ttccccaaca caaactaatt tcattcggca aggttgacga tgttgtgtaa ccgcagtata
     6541 cattgcagta aatgaagatt tatacacttt cacattttct gccccatatt gctcaatcgc
     6601 ttttggctca cttaaaccaa cagtacctat tggtgggtgg ctaaaaacga ctgtcggtac
     6661 taagctataa tccaaatgtt catttggttt attattgaac aaccgttccg atagcctgcg
     6721 accagctgca actgcaacag gagttaattc aataccacct tcaataatat caccaacagc
     6781 atagatattt ggcacattag tattttggaa tttatccact ttgataaaac cacgctcgtt
     6841 ggtttcaaca ccagcagcct ctaaattaat cttatctgtc gctggctcac gaccaatagc
     6901 ccacaccaag ctttcaacag aggtttcacg accatcttgc aattttaatg tcaatgaacc
     6961 atctgcattt ttaactactt cttgaggagt cgcttgtgta tgtaattcga tcccatcttg
     7021 agctaatact tcaagcaatg tatccactat taatgggtct tgattacgca ttggagcatg
     7081 ttgacgaaca aaaagatggg tatttactcc taaactatta aatactccag ctaattcaac
     7141 tgcaatatac cctgccccca caattgctac ggacttcggt aaagcatcta gtgcaaatac
     7201 accatcagaa gtgataccgt attcagcccc tttcacattg ggtacagttg gacgaccacc
     7261 tgttgcaatc agaatatggt ctgctgtgac ctcctcaacg gaaccatcag cataactcac
     7321 ttctaatgtt ttagcatctt taaagcgagc aaaaccattt aaaacatcaa cattattttt
     7381 tgccagcaca ttaccatatg aggtatgaat gcgcccaata taggcctggc gactctcaac
     7441 taatttggca taatcaaaat tatttaccgt aatgtcaaaa ccataatcag gggcgtaatg
     7501 gttaatcgct tctgcaactt gtgcaccata gaacatcact tttttgggta cacatcccac
     7561 gttcacacaa gtgccaccta aatgttttgc ttcaataatg gcacattttt gcccatagct
     7621 tgctgctcga ttaattgaag caataccacc actaccaccg ccgatagcaa tataatcata
     7681 atgtttagcc ataagctatt cctcgatcta taaaaacaaa agcaacttat tcgttaagcg
     7741 ctttatcaaa ttaaatttgc atagaagact aatgtttcat atgccctgaa ttcaacattt
     7801 tatcaatata cgccatcgct aatgctgaaa caataaaggc taagtgaata actacttgcc
     7861 acatcatttg cttttcatca agatttgaag cattgataaa tgtttgtaat aaatgaatag
     7921 aagaaatact gataattgcc attgaaagtt ttactttcaa aatagtcgca ttaacgtgat
     7981 ccaaccattc tggctgatca gggtgatcat ctacttttaa tctagagaca aaagtttcat
     8041 atcctccaac aataaccata accaaaagat ttgcaatcat caccacatct attaaattta
     8101 ataccgctaa cataacagta tttgaatcca tttcatttaa attgataact agatgataca
     8161 atgatttaat aaacttatac gcatatatcc cctgtacaac aatcaaccct aaataaatag
     8221 gtaattgtaa ccaacgactt gcgaaaataa ttttacctaa aatatttgcc ctagtattta
     8281 attccgaatg catatatctc ctataaatag caatacaaaa ataaattaca acgctcaaat
     8341 tttaacaatt ttttgccaaa aaattaagat atttaaccgc ttgcacctta tattttaaaa
     8401 ccttgtccat cgttccaaga aaccgtatat tctagaggta aacggcaact tggcaacgct
     8461 gggtttgctg ctttaccgat agctagtagc ataacaggca aataacgttc tgcatctata
     8521 ttcaataatt ttaatacagc agggcgctca aacacgccta tcgcatgagt atcatagccc
     8581 ttatctttgg caattagcat taattgcatt gccgccaaac tagtatcaat taatacctga
     8641 tctctaattt ctgcttgagt tgcttggtta tgaagattta gtaaaaaatc aaatttacgt
     8701 tcacgaaact ctttatgcaa acacccagtt tctaccgatt gatcaacaat tgcgcctaat
     8761 aatttttcat attgcaaatc tgccaatatc accacaatag cagacgctgt ttcacaaggt
     8821 gggccattaa aggcaacatt ttttgataat gctgctttaa gaatttcatc atcaataatg
     8881 acaaaacgcc aaggttgcag atttgctttt gagggagcaa gctgggataa agcgaccatt
     8941 tcttctaatt cagttctact aatcttaatt gttgaattaa aagctttgat agttttacgc
     9001 tgttcaatcg tatgtttcaa ttccatctct tttctcctat ctttcaaaca attgagatag
     9061 tctataattt aaagattagg aatgtaataa ttgaaaagag ttattctgca tatttattga
     9121 aataggtcaa aattatcaag taatcatatt agagcaggag taaagattta gcttgcccaa
     9181 gcataggaac taaggtgctt tccaaaatga ctttcaatta aacgtttcgt aatttgatgt
     9241 tgcggattca gtaaaacctc tttcgtcaag ccatactcta ctacttcgcc actttgcatc
     9301 accattattt tatcggcaat atgtttgatc agacctaaat tttgccccac ataaatataa
     9361 gcaatccctt gtcgctgctg gacacttaac attaaattaa ttaattgtgt tttgactgaa
     9421 aaatccagtg aattaatcgt atcatccgca ataataattt ccggttctaa aattaaagca
     9481 cgagcaaacg caatacgctg cttttgccca ctggaagcct ctgaaatagg aacgagcgct
     9541 tgctcaggat acattcctac caattttagc gtattaaaaa tacgctcatt tctttcctct
     9601 tcatttaaat ctgtcgccaa gcggagcgga gcatctaaaa tttgtcctat attataatta
     9661 ggatcaaagg catcgttagg gtcctgaaac atcatacgaa tatgcttagc tcggtattta
     9721 taatcgccaa atgaaagcgg tgttcctcta aaaatcagtt ctccagatgt tggctcaacc
     9781 ataccagcaa ccattttagc caaggtagat ttcccagctc cgttttcacc aataatcgct
     9841 aaagtttcct tgcgatttag ggtaaaagaa acttctttta ccgcataaaa atcttgttta
     9901 tgaaataggc tactacgttc aacaaaacgc ttattcagat gattaacttc aagtaatgcc
     9961 ataataaatt ggtgcttgga aataaagtga tagtttaccg caaaaaataa ataaaaaaag
    10021 accgcttata caagcggcct gatttatgga attttttgca aattataaaa cgttcacaca
    10081 gtttaagtct tcgaaagatt gctctaagcg tttagacata gactcttcca ttttacgtaa
    10141 ccaaacacgt ggatcgtagt attttttgtt tggtgcatca gggcctgtcg ggttacctaa
    10201 ttgaccttgt aaatatgctt cgtttgcttt gtagaattga agaataccgt cccacgcagc
    10261 ccattgggtg tcagtgtcaa tgttcatttt gattgcaccg tagctgattg cttcacgaat
    10321 ctcttcacgt gaagaaccag aaccgccgtg gaaaacgaag tctaatgact ttgccggtaa
    10381 accacgctct ttagatacat actcttgtga agcacctaag attgatggtt ttaattttac
    10441 attacccggc ttgtaaacac cgtgtacgtt accaaatgct gccgcaatcg tgaaacgtgg
    10501 gctgattggg cttaattggt cgtaaacata taaaacttca gaaggttggg tgtaaagttt
    10561 agattcatct acatcagaat tatctacgcc atcttcttca ccaccggtta cgccgatttc
    10621 gatttcaagg gtcataccga ttttgtccat acgggctaaa tattcacggc agatttccat
    10681 attttcttcg attggctctt cagataaatc gatcatatgt gaagagaata acggacgacc
    10741 ggtttctgcg aagtgttttt cgcctgcttc aagtaagcca tcgatccaag gtaataagtt
    10801 tttcgcagcg tggtcggtgt gaagaatcac aggcacacca tattcttctg ctaattgatg
    10861 aacgtgtttt gcacccgcaa tcgcccctaa tacatctgca cgagcgcctg aagccggctt
    10921 aatgccttta cccgcataga actgcgcacc accatttgag aattgcacga ttaccggtga
    10981 ttttacacga gcggctgttt ctaatacggc attaactgaa tctgaaccca cgcagtttac
    11041 tgccggaata gcgaagttgt gttctttagc ataagcgaaa actttttgta cgtcgtcgcc
    11101 tgttactaca cccggtttta cgatgtttaa taatgacatt ttttgcttcc tttgtatgaa
    11161 atgtgcaaaa gcacattgtt aaaatatacg gccaaatctt gcaaaatttt cacaaaattt
    11221 gaccgcttgt aactgttagc cgttagctcg tttttctaag atttccactg ccggtaatac
    11281 tttaccttcc acgaattcta agaacgcacc gccaccggtt gaaatgtaag aaattttgtc
    11341 tttgataccg aataaatcaa tcgcagctaa ggtatcgcca ccaccggcaa ttgagaatgc
    11401 accgtttgcc gttgcttctg caatcgcatt tgaaatcact tctgttcctt tacggaagtt
    11461 agggaactca aacacgccga ccggaccatt ccaaaggatg gtttttgaat tacggataat
    11521 ttcagctaat tgctctgccg atttttcacc aatatcgaaa attgattcat cagctcgtac
    11581 ttcactcacc gctttttccg ttgccggtgc agtttcagag aattcagtac ctacacgcac
    11641 atcaaccgga acaggaatat tggttacttg tgctaaacgt tttgcttcag ggattaaatc
    11701 ttcttcgtat aatgatttac ccacagcgtg accttctgcg gcaataaagg tattggcaat
    11761 accaccgccc acgattaatt ggtcggcaat ttttgaaagc gaatcaagca cagttaattt
    11821 agtagatact ttagaaccac ccacaatcgc taacattgga cgttgtggct cttttaatgc
    11881 tttgcctaat gcatctaatt cagcggctaa taacggacct gcacacgcaa caggggcata
    11941 ttctgccaca ccgtaagttg aaccttccgc acggtgagct gtaccgaatg cgtccattac
    12001 aaacacatca caaagtgatg catatttttt tgctaactca gggtcgtttt tcttctcacc
    12061 tttgttaata cgcacgtttt caagcaccac gatttcattc tcgtttactt caacaccgtc
    12121 taagtaatca cgcactaaac gaacaggaac acctaaatct gatgcattta agtaatcaac
    12181 tacaggttgt aaagagtttt cctcttcaaa tacgccttct gtcggacgac ctaaatgcga
    12241 agttaccatt actttagcac ctttttgtaa cgctaatttt aaggttggaa tggtagcaac
    12301 aatacgggca tctgatgtta ctttgccatc ttttaccggt acgttaaggt cagcacgaat
    12361 aaataaacgt ttacccgcta aatcaaggtc agtcatttta ataacagaca tagtttgttc
    12421 ctctttagat taaaatttaa aagtgataac ggtcggatta tatcagtttc taatcatttt
    12481 gggctaacct agatcaaata aatgattaat tttttatcat tttagaatgc tattatcctg
    12541 actgttctta tttccccccc aatatgcttg gttaattaag caagaattgc aaattcccca
    12601 caaaatttaa ccgcttgtca ctaaaaataa agctgtttga aaatccattc aaacagctta
    12661 tagaatttag agtgaattaa agaaaatcac caagataatc accggaatca caaagcgaac
    12721 atagttaaac caaatagtgg taaacatttt acccgcacct aattcctcct tcgcctcttc
    12781 ttttagaaca aagccaacga aaatcgcgct acctaaggcg gttaaaataa acagaatatt
    12841 gccactaata taatcgaagg catcaaaaat gctcttgccc ataacctgca catcttgcaa
    12901 tatattgtca cttaatgctg atggaatatt acccaacaga aaaatagtag ccaaagtaat
    12961 aaaaatagct ttttgacggc taatctttgc cttctcttgc aatgcagtga taataacttc
    13021 ataaatagtt aacgaggttg ttaatgccgc caccactaat agactaaaaa agataaccgc
    13081 aaaaactgtt cccccccaca tatttgagaa cacaatcggc aaactttgga atactaaagt
    13141 cggacctgaa tttggagcaa cgccaaagct aaataatgac ggaaaaatca taaaaccggc
    13201 aagtaccgca attaaggtgt tcatcacccc agtaattgtt gctgttttta ctaaattctc
    13261 gctcttatca aggtagcttg aaagggttat caacacacca aaaccaaggc ttaatgcaaa
    13321 aaatacttgc cccagcacaa acacaaacaa ttttggtgtg atttttgaaa aatcgggcat
    13381 cagataaaag gcaatgcctt cagcagcccc cggtaaggta atatttcgaa ccaccatcac
    13441 aatcaggaaa ataaacagaa tcggcatcaa atatttcact gaacgctcaa taccgtccac
    13501 aatgcctttt accaaaataa aataattcac caatacaaaa agtgccgtat aagcaataat
    13561 tgtcattggg ctattaaaaa tactttgttg gaagaattgt tgggtcgtgc caacggtaac
    13621 aggttgagac agatctagtg tccccgtgag taagccaccg atataattta atacccaacc
    13681 accaagtacc atatagtagg caagaatacc aaatgctcca agcaagccca tatagccaag
    13741 aattttccaa cctgaggcaa tttttttacc attagcaaaa ccggcaaaag catcaatagc
    13801 attcactctc attcggcgac caattacatt ctccactaat atcataggga taccaattac
    13861 gatcatcgca atacaaaata aaaacacata agcaccgcct ccgtgttccc ccacaagata
    13921 agggaaacgc caagttgcac caaaaccgac tgttgcacca gcaacggtca taatatacgc
    13981 caatcggtta gaccaagatt ggcgttcttg ttgttgattt gccatttaat actccatcgg
    14041 ctaacaatac cgcttcaacc aacaagcggt cagattttta gaaaatatta caaattaaat
    14101 gaagagtgcg aataactcgc caactttaac ccttgagccc accttctgcc ctattgaacg
    14161 ctgtacctga atttgggtaa tatcggcaga ttgataaatc ctacgggtaa aatcagtatc
    14221 gtgatttgaa attaatacgg gaacgcctaa tcctttcatt tctaaagcca gtgttgccaa
    14281 acgcccttgt tgctctaggc taaaaccacc gccagcatat tgcgtaaaat tcgtttcttg
    14341 tgctaaagga gcgtaaggtg ggtcgcagta aatcacataa tccgttaatt gattttttgc
    14401 caactcaaag acctgttcaa aatccgcaca cacaaaagtt gccttttgtg ctttttcagc
    14461 aaaaaaacgt agttcttttt ctggaaaata atgggtttta taacgcccaa agggaacatt
    14521 gtaccctttt ttctggttat aacggcaaag tccgttatag ccaaatcgat ttaaatacaa
    14581 aaaaatcacc gagcgacgaa aaatatcttt tgagcgatta aactccgcac ggcatgcttt
    14641 ataaaaggct tctgtattcg cttgtgggtg aagaaacagt tgctttgttt gcgcaatata
    14701 ttcctcaaca tctgctttca ccacgttgaa aagattaatc aagtcaggat taatatcagc
    14761 aagaatataa cgttcaaaat ctgtattcag aaagaccgaa cctgcaccaa caaaaggctc
    14821 gactaaacaa gccttttttg gcaaatgggt ttgaatatct ggaattaagc gatatttacc
    14881 gccagcccat tttaaaaagg ctcgatgctt agggtgattc atttcatttt aaacattaac
    14941 gttgggtaaa acgctcaata gaggctaata cttgagattc tgtggcttgc gatgaaacgt
    15001 aagcttcacc aagtgatttc aataaaatta ggcgtaattg acctgccagt acttttttat
    15061 ctcgccacat ataaggtaga tattctgccg gttccattcc atcaggtgaa attgttggca
    15121 aattggctcg ggccaataat ttttccaagc gagcaacctc ttgaggactt aaatccccca
    15181 gaatacgaga taattccgca gcctctagca taccaaccga aacagcctct ccgtgtaacc
    15241 atacaccata gcccatacgt gcttcaatgg cgtgaccaaa ggtatgtcct aagtttagta
    15301 atgcacgatc tcctttttcc gtttcatctc gagcaaccac gtcagctttt agctggcaac
    15361 aacgctgaat acaatattcc agctcatctt gcttgagact gaccaaatca tcaatatgct
    15421 tttcaagcca ttcaaaaaaa gtaacatcaa agattgcacc gtatttgatg acttccgcca
    15481 aaccagcact gacttctctt ttggataagg tatggagcgt attggtatca acaatcacag
    15541 aaacaggctg ataaaaagcc ccaatcatat ttttaccgag tgggtggttt actgccgttt
    15601 tcccgcctac tgaagaatca acttgagcca gtaaagtggt cggaatttgt ataaaacgaa
    15661 taccacgttg gtaagaggca gctgcatagc ctgcaacatc accgattaca ccaccgccta
    15721 aagcaattaa agtggtatcg cgattatgat ttttttctaa taatgcggta aaaatcaaat
    15781 ttaatgattc tagcgtttta tattcttcac cgtcgggaat caataccgaa tcaacgtggc
    15841 aacccagctc ctttaacgca tttgttacca cactaagata atgtgtcgcc accgttggat
    15901 ttgagacaat catcacctta tctccagatt ttaatgggct ataactagca ggctgattta
    15961 ataaccctgc accaattata atcgggtaac gacgctcttt taattccaca ttgacttgta
    16021 acatatctta gctcctttct tacctggcta ctgtaaatta tcaatcatat caataatttg
    16081 atttgccact actttagcgc tctgctcgtc cgtatgaata gtaatatctg caatttcttc
    16141 atacaatggg ttacggattt tcgctaactc ttccaacgtt ttacgggggt cttctgtttg
    16201 caataaaggg cgttttttat ctcgctgagt acgttcaaac tgtttatcca ctgtcgtttc
    16261 taaataaata acgataccac gagccgataa cacattacgg ttatctttag ataaaatcga
    16321 accaccgcca gttgaaagca cgataccata attttgcgtc agttcattta gaatacgttc
    16381 ttcacgctta cggaaaccct cttcaccttc aacatcgaag atccaatcaa tatccgcacc
    16441 tgctcgttct tcgatcacac tgtcggaatc cataaattcc atacctaatg tttgagcaag
    16501 ctgacgacca atcgtacttt tacctgctcc catcggccca atcaaaaaaa tattacgttt
    16561 ttcagccatt atttcttctt cattttaaaa tttactaaac atttgatctg tgaaattaaa
    16621 agaaaaaatg accgcttgtt ctacaagcgg tctgatttct ttaaaaaatt gcaataaaac
    16681 ccatttattg actaaggaat atacaaatat aactcacaaa tcgcccaaaa taaaattggc
    16741 acttattatc gcaagaaatc c
//
LOCUS       DANJWC010000040        16753 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.040, whole genome shotgun sequence.
ACCESSION   DANJWC010000040 DANJWC010000000
VERSION     DANJWC010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16753
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.040"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(364..879)
                     /locus_tag="PWX02_000950"
     CDS             complement(364..879)
                     /locus_tag="PWX02_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL1728979.1"
                     /translation="MAGINKVIIVGNLGNDPEMRTMPNGEAVANISVATSESWTDKNT
                     GERREVTEWHRIVFYRRQAEVCGQYLRKGSQVYVEGRLKTRKWQDQNGQDRYTTEIQG
                     DVLQMLGSRQGNDMGGQQGGWGNSAPAGNSYNQGNSNYGYNQTQSAAPQSKPAPQPEP
                     AMNNFDDDIPF"
     gene            1034..3862
                     /gene="uvrA"
                     /locus_tag="PWX02_000951"
     CDS             1034..3862
                     /gene="uvrA"
                     /locus_tag="PWX02_000951"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDL1728980.1"
                     /translation="MQHIDIRGARTHNLKNINLTLPRDKFIVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTVT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTISQMVDRVLEEPEGKRMMLLAPVVKERKG
                     EHVKLLENLTASGYLRARIDGEICDLSDPPALELQKKHTIEVVVDRFKVRADLATRLA
                     ESFETALDLSGSTAIIADMDDPNAEEIVFSSSFACPHCGYSLHELEPRLFSFNNPAGA
                     CPTCDGLGVEQFFDAKKVVQNEEVSLAAGAIKGWDRRSFYYFGLLKAVAEHYGFDLET
                     PFGQLPKKYQNIILNGTKEEIEFVYVNDRGDKVKRKHAFEGVLNNMARRYKETESNAV
                     REELAKYINNRPCMDCEGSRLRREARYVFLDKTNLPMVSEKSIGEALTFFEELKLAGQ
                     KEKIAEKILKEIRERLQFLVNVGLNYLSLSRSAETLSGGEAQRIRLASQIGAGLVGVM
                     YVLDEPSIGLHQRDNERLLNTLVHLRNLGNTVIVVEHDEDAIRAADHIVDIGPGAGVH
                     GGQVIAQGTAAEIMQIEESITGQFLSGKQKIEIPAQRVPYEPTKLLTLSGASGNNLKK
                     VKLEIPVGLFTCITGVSGSGKSTLVNDTLFLLAQNALNRAENSDVAPYESIDGLSHFD
                     KIIDIDQSPIGRTPRSNPATYTGLFTPIRELFAGTQEARARGYNVGRFSFNVRGGRCE
                     ACQGDGVIKVEMHFLPDVYVPCDHCKGKRYNRETLEIRYKGKTIHQVLEMTVEEAREF
                     FAPVPMIARKLQTLMDVGLSYIRLGQSSTTLSGGEAQRVKLATELSKRDTGKTLYILD
                     EPTTGLHFADIKQLLGVLHRLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGEI
                     IATGTPEEVAKDKRSHTARFLKEILAKG"
     gene            complement(3954..5687)
                     /gene="dauA"
                     /locus_tag="PWX02_000952"
     CDS             complement(3954..5687)
                     /gene="dauA"
                     /locus_tag="PWX02_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HDL1728981.1"
                     /translation="MLNKWFLTKNAFLAVKPFSALKDALNAGYGKQHLIRDIISGLTV
                     GVIAIPLSMALAIASGVPPQHGLYTAIVAGIVIALTGGSRFNISGPTAAFVVILYPVT
                     QQFGLSGLLMATMLSGGILVLMALLRLGRLIEYIPLPVTLGFTCGIGIVIATLQIKDF
                     LGLSIEKMPEHYLGKLEAIFSALPTINWADTLVGVVTLLVLTRWHKLRLPIPGHLPAV
                     FIGTLLSLLLIHFGQNVATIGSAFHYTLSDGTTGYGIPSVLPEFVLPWNLPNAEGNRI
                     DWNLTTIQALLPAAFSMAMLGAIESLLCAVVCDNMTDTKHHSNNELLAQGLGNIASPF
                     LGGITATAAIARSAANVRSGAVSPISSVVHALLVLFALLFLAKALSYLPLSSMAALLL
                     VVAWNMADIPQIIQLTRRSGKNEIAVLLTCITLTVLFDMVIAITVGVLLASLLFIRTI
                     AEMTKSFEMAHPTDLNDVLVYRISGPLFFAAADNLFADLHNKTVHTDHQIKHIVLQCD
                     AVTVLDAGGIHALTRFVQHMLPHQQLHLCNVQFQPMRMLVRSQKTVNEIPQIQFSSDL
                     ESAFDKIRQAN"
     gene            5929..6861
                     /locus_tag="PWX02_000953"
     CDS             5929..6861
                     /locus_tag="PWX02_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1728982.1"
                     /translation="MDSLIHFLSAFLTNITWIGALELGLIYSLVALGVLISYKILDFP
                     DLTADGSFPLGGAVCVISILNQVDPWLATLYGTLAGAVAGMVTASLHIGFKIEKLLAS
                     ILMMIALYSINLRIMGMPNIAILGETTIYDVMPIESDLQLALVRLVMTLLVVIIVKGL
                     FDLFFATEIGLAVRATGTNSRMAKAQGIAINKMTILGMAISNALIALGGALYVQSNGG
                     VDISIGVGTIVIGLAAVIIGEAIFSVKRIIWLTFAVIIGSILYRFFIALALNNETLNG
                     IGFGPQDLNLITALLVVIVLVVPRIKHKLLGKRG"
     gene            6863..7660
                     /locus_tag="PWX02_000954"
     CDS             6863..7660
                     /locus_tag="PWX02_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1728983.1"
                     /translation="MIELNNLFITFNKGTAIENPVLRGLSLKVEQGEFVSVIGSNGAG
                     KSTLLNAISGDCAVDSGEILIQGNKVNQSTTWQRANQVARVFQDPMAGTCESLTIEEN
                     MALAYQRGRKRGLGFAIKRQMRELFKDKLALLGLGLENRLTDQMGRLSGGQRQAVSLL
                     MASLQPSNVLLLDEHTAALDPKTTDFVLELTNKIVREQKLTTLMVTHSMRQALDYGDR
                     TVMLHQGQVAFDVSGEQRQKMDVPDLLQLFQQSRHEQLSDDGLLLGN"
     gene            7747..7968
                     /locus_tag="PWX02_000955"
     CDS             7747..7968
                     /locus_tag="PWX02_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1728984.1"
                     /translation="MLSIFKTPVEKETLDDWAKISVDVAKVAILAIPVILFGKEPTLI
                     KLTNLALLVLSIYVFLTLGRKLRQLKEVL"
     gene            8151..10076
                     /locus_tag="PWX02_000956"
     CDS             8151..10076
                     /locus_tag="PWX02_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDL1728985.1"
                     /translation="MTKYDDKIQHAFSENGLLSQNISGFRPRQAQLEMAQVVAKAVKF
                     ATPVVVEAGTGTGKTFAYLVPALLSGKKTILSTGSKNLQDQLFNRDLPTIQKALKYKG
                     KIALLKGRANYLCLERLDQVTAMGVLGDKSVLTDLKKVSKWQNATKTGDLSECITIAE
                     DSPILPQLVSTTESCLGSDCPHFKDCYVVQARRKAMEADVVVVNHHLFCADMAVKETG
                     FGELIPEAELVIFDEAHQLPDIASQYFGQSLSSRQLFDICKDTNIVYRTELKDLSQLG
                     KAADHLTKVVQDFRLLMGGEGQIRGNLRELFRDKKVVDGLVKVAENIDFLSEVAKKSL
                     GRSEMLDKIFERLAEVKVQLKKLMDTSVTGYCYWYEANGRSFGLHITPLMVADKFGEQ
                     LKNQQIGWVFTSATLEVGGKFDHFCQRLGIENAEQLVLQSPFDYQHQSLLCVPRYLPD
                     TNKSNTLTALGQLLKPVIEANKGRCFLLCTSYYMMRGLADFLREHSSLNVLLQGETSK
                     SRLLEKFVKEDHSVLVATQSFWEGIDVRGDDLSLVIIDKLPFTSPDEPLLKARMEDCK
                     IQGGDPFNDIQIPEAVITLKQGVGRLIRDVTDRGVVIICDSRLVMRNYGQTFLKSLPP
                     SRRTRDLGNVIQFLKQK"
     gene            10150..10515
                     /locus_tag="PWX02_000957"
     CDS             10150..10515
                     /locus_tag="PWX02_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDL1728986.1"
                     /translation="MLSTLLRMVASLALLFVMLYLGKLIVYLFPIGIPDSILGMLILF
                     GCLVIGIVKVEWVIPSAKPLTRYITLFFLPICVELIEHLELLRQNLNTFVLATALSTL
                     VSVVAIGVFAQWIFHYKRG"
     gene            10517..11203
                     /locus_tag="PWX02_000958"
     CDS             10517..11203
                     /locus_tag="PWX02_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDL1728987.1"
                     /translation="MSYLYTVLTVIMFIAGLKISQKLKSSFLNPFLIALALLIGVLLI
                     FNIPYQDYYQGNSPLNNLLGVSVVALAYPFYEQLPHIRKKWRQIVVILLFSTIITMIT
                     GLGFSLLFGASQEILAAILPKSVSMPMAIIITNEINGNVAIAAVGVMIAGLVGSILGF
                     TLLRLAKVRNVMAIGLSMGAASHALGTARCLEHSMKAGSYSSIALVACGVLSSIFAPL
                     IFNVIVTLCY"
     gene            11287..13050
                     /gene="msbA"
                     /locus_tag="PWX02_000959"
     CDS             11287..13050
                     /gene="msbA"
                     /locus_tag="PWX02_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HDL1728988.1"
                     /translation="MQEQITQEKDLSTLKTFKRLWPTISPFKAGLITGAVALVLNALV
                     DSSLIYLLKPLLDEGFGQANNDFLKLMAVLVVVFILVRGITNYIASYCLAWVSGKVVM
                     TLRRHIFQHLMYMPVSYFDNNPTGRLLSRVTYDTEMVASSASHSLVTIVREGAYLLSL
                     FVVMIYTSWQLSIVLFVMAPIIGVLISFVSKYFRQLSRNIQSSMGELTVTTEQMLKGH
                     KVVLSFGGQEIEKARFDRVSNDMRRKGMKVVSADSISDGVVQLIASFALSAVLYVATF
                     PEVMSENLTAGSFTVVFSSMMAMLRPLKSLTNVNSQFQRGMAACQTLFEFLDLKTEKD
                     HGSISVVKAEGNVEFKNVSFSYEGKDEKALNNISFTIPKGKTVALVGRSGSGKSTIAN
                     LLTRFYDVTDGEILLDGINIQDYRLKNLREQCSVVSQQVHLFNDTVANNIAYAATDKY
                     TREEIIVAAKAAHAMEFIEKLENGLDTVIGENGASLSGGQRQRLAIARALLRNSPVLV
                     LDEATSALDTESERAIQSALEVLQKDRTVLVIAHRLSTIEKADEILVVDHGNIIERGS
                     HTELLAQGGAYKQLHSLQFGA"
     gene            13139..13771
                     /gene="lolB"
                     /locus_tag="PWX02_000960"
     CDS             13139..13771
                     /gene="lolB"
                     /locus_tag="PWX02_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HDL1728989.1"
                     /translation="MKKLTKLLSLSALLVLTACNSVLNAPTEVQQPTVQIPHNDPQWQ
                     QHLAKLSQIQGYTATGQFGYISPEERFSSHFNWRYTNPIQFGLELSSNLSTKSLKLER
                     NKRGLTVSDSEGNSRSDRDVDALMQEIISVSFPIDQFAYWVKGLPEKDGNYIVNEKRQ
                     LSQFSYPLNGTLWKASYVEYHEDRVPNLPKLIVLENGTQTLKIRIEKWAY"
     gene            13759..14613
                     /gene="ispE"
                     /locus_tag="PWX02_000961"
     CDS             13759..14613
                     /gene="ispE"
                     /locus_tag="PWX02_000961"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDL1728990.1"
                     /translation="MGVLMKKVTLPSPAKLNLFLYITNKRADGYHELQTLFQFLDFGD
                     EIEIEITESSEVELINPVEGVATEENLIYRAAKLLQKTTACRQGCRIGIKKNLPMGGG
                     VGGGSSNAATVLVGLNHLWNTELSLEKLAELGLSLGADVPIFVRGMAAFAEGVGEVLT
                     PCDPQEKWYVVLKPEVSISTEAVFTDPNLPRNTPKRPLAELLSLPWENDCEKVVREKY
                     SAVEDLIQELLQYAQFRLTGTGACIFAEFETKAEAEQVFAHKPEAVFGFVAKGQNISP
                     LHQMLFQI"
     gene            14639..15589
                     /locus_tag="PWX02_000962"
     CDS             14639..15589
                     /locus_tag="PWX02_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HDL1728991.1"
                     /translation="MTDIKLFAGNATPELANRIAKHLYTSLGNATVGRFSDGEIQVQI
                     NENVRGGDIFIVQSTCAPTNDNLMELIVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVIADFLSSVGVDRVLTCDLHAEQIQGFFDVPVDNVFGSPVLIDDILKKS
                     DLVNPIVVSPDIGGVVRARAVAKLLNDTDMAIIDKRRPKANVSQVMHIIGDVADRDCI
                     LVDDMIDTGGTLVKAAEALKERGARRVFAYATHAVFSGSAAQNLANPALDEIVVTDTI
                     PLSNEIKALDKVRVLTLSGMLAEAIRRISNEESISAMFDA"
     gene            15668..16678
                     /gene="pyrD"
                     /locus_tag="PWX02_000963"
     CDS             15668..16678
                     /gene="pyrD"
                     /locus_tag="PWX02_000963"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDL1728992.1"
                     /translation="MYSLIRKLLFALDTEKAHQFSIQALALAGKSPFSILPTPDNPTQ
                     VMGLTFKNPIGLAAGADKNGEAIDGFGKLGFGFIEVGTITPLAQDGNPKPRQFRIVDA
                     EGIINRNGFNNKGVDVLVENVKNATYSGILGINIGKNAVTPIEHSFDDYQICLRKVYQ
                     YADYVAVNISSPNTKNLRSLQYGEALDDLLRNLKNEQAQLSQKMGKYRPLVLKIAPDL
                     TDEEIVSVADSLIRYQIDGVIAGNTTLSRESIAGLPYSEQLGGLSGKPLHSLSTRLIS
                     KLSAELGERIPIIGCGGVHSLESGQEKVNAGASLLQVYSAMIYQGPSLIQTLAKHLQL
                     NS"
BASE COUNT         4829 a         3177 c         3859 g         4888 t
ORIGIN      
        1 gccgcccata aagcgtgtcg gttcgagtcc gaccactggc actactctaa tatcttttct
       61 tatttactat taagaatttc tctattatct tcctatatca atttttctca aaatattgta
      121 tttataaacc cgtatagatg ggaactttag ccgtttatgt gagaaatggg gcaataataa
      181 atctgtcaat aagttactta ttaatcgaat gaaaagtgga tttattaatt aatagggaac
      241 acttattact tggaagtgtt ccctattatt ttgagtaagg taggggcgct ttatatgagt
      301 agctttttat ttcatcgaaa tgcggtgtat ggaacccatg agctacttaa gtcaatactt
      361 tgattagaac ggaatatcat catcaaaatt gttcattgcc ggctcaggct gtggtgccgg
      421 tttagattgc ggtgcagcac tttgggtttg gttgtaacca taattgctgt tgccttgatt
      481 gtagctatta cccgcaggag cagaatttcc ccaaccgcct tgctgaccgc ccatatcatt
      541 accttgacgg ctaccgagca tttgtagcac atcgccttga atttcggtgg tgtaacgatc
      601 ttgcccgttt tggtcttgcc atttacgggt ttttaaacgt ccttctacgt aaacttgaga
      661 gcctttacgt aaatattgcc cgcatacttc tgcttgacgg cggtagaata caatacgatg
      721 ccactcggtt acttcacggc gttcaccggt gtttttatcc gtccagcttt ctgaggttgc
      781 cacactaata tttgcaaccg cttcgccatt tggcattgta cgcatttcag ggtcgttacc
      841 taaattaccg acaataatta ctttattgat accagccata aaatcctctg tttgataaat
      901 taaactaaaa tttgctaagt attctacctg aaaatccaaa agatgaacac caaaaaactg
      961 atattttgta cagttttatt aaaatatgcg ataatgatcg aaaattgaga aaatagcact
     1021 ggacattaaa aatatgcaac atatcgatat tcgtggagca agaacacaca acttaaaaaa
     1081 catcaattta accttgccga gagataaatt cattgtaatt accgggcttt ccggctcggg
     1141 caaatcgtct ctggcgtttg atacgctcta tgcggaagga caacgccgtt atgtagaatc
     1201 actgtctgca tacgcccgcc agtttctctc cttaatggaa aagcctgatg tggatcatat
     1261 tgaagggctt tcccctgcga tttcgattga gcaaaaatca acctctcaca acccacgttc
     1321 gaccgtagga acggtaacgg aaattcacga ctatttacgt ttattgtttg ctcgagtggg
     1381 cgagcctcgt tgccctgacc acgatgtgcc attggctgct caaactattt cgcagatggt
     1441 ggatcgagta ttggaagaac cggaaggaaa acggatgatg ctacttgccc ctgtggtgaa
     1501 agagcgtaaa ggcgaacacg taaaattatt ggaaaatttg accgctagtg gctatttgcg
     1561 ggctagaatt gacggtgaaa tttgcgattt atccgaccca ccggcattag aattacagaa
     1621 aaaacacacc attgaagtgg tggtggatcg ctttaaagta agggctgatc tagccacccg
     1681 tttggcggaa tcttttgaaa cagcgttaga tttatccggc tctaccgcaa ttattgcaga
     1741 tatggacgac ccaaatgccg aagaaattgt gttttcgtcc agctttgctt gtccgcattg
     1801 tggttactct ttacacgaat tagaaccacg ccttttctcg tttaacaacc cggcaggtgc
     1861 ttgcccgact tgtgatggtt tgggcgttga gcagtttttt gatgccaaaa aagtagtgca
     1921 aaatgaagag gtttcattag ccgccggtgc gatcaaaggc tgggatcgcc gcagtttcta
     1981 ctattttggc ttattaaaag ccgttgccga gcattatggc tttgatttag aaaccccatt
     2041 cgggcagcta ccgaaaaaat atcaaaacat tattttaaac ggcactaaag aagagattga
     2101 gtttgtttat gtcaatgacc gaggcgataa ggtcaaacgt aaacacgctt ttgaaggggt
     2161 gctgaataat atggctcgcc gctataaaga aaccgaatca aacgcggtgc gtgaagagtt
     2221 ggcgaagtat attaataatc gtccttgtat ggattgcgaa ggctcacggt tacgccgtga
     2281 agcccgttat gtattcttag ataaaaccaa tttgccgatg gtttcggaaa aaagtatcgg
     2341 cgaggcatta accttttttg aagagctgaa attagccggt cagaaagaaa aaattgctga
     2401 gaaaatcctc aaagaaattc gtgaacggct acagttttta gtgaatgtcg gcttgaatta
     2461 tctttctctt tcccgctcgg cagaaaccct ttcaggtggc gaggctcagc ggattcgcct
     2521 tgccagccaa attggtgcgg gtttagtagg ggtaatgtat gtgctggatg agccttccat
     2581 cggcttgcac caacgagaca atgaacgcct actgaatact ttggtgcatt tacgcaattt
     2641 aggcaatacc gtgattgtgg ttgaacacga tgaagatgcg atccgagccg cagatcatat
     2701 cgtggatata ggcccgggtg ccggtgtaca cggtgggcaa gttatcgcac aaggtacggc
     2761 agctgaaatt atgcaaattg aggaatctat taccggacaa ttcctctcag gcaagcaaaa
     2821 aattgagatt ccggctcagc gtgtgcctta tgagccaact aaattgctca ccttatcggg
     2881 agcaagtggt aacaacttga aaaaagtgaa attagagatt ccggttggtt tatttacttg
     2941 tatcacgggt gtatcaggtt cgggcaaatc aactttagtt aatgacacac tattccttct
     3001 ggcacagaat gcgttaaacc gagcggaaaa cagcgatgtt gctccttatg aatcgattga
     3061 cggcttatcg cattttgaca agataattga tattgaccaa agcccgattg ggagaactcc
     3121 tcgctcaaat ccggcaactt acaccggatt atttacgcca attcgtgagc tttttgccgg
     3181 cacgcaagaa gctcgagcca gagggtataa cgtagggcgg tttagcttta atgtgcgggg
     3241 cggacgttgc gaagcctgtc aaggtgacgg cgtgattaaa gttgaaatgc acttcttgcc
     3301 tgatgtgtat gtgccttgcg atcactgtaa aggcaaacgc tataaccgtg aaaccttgga
     3361 aattcgctat aaaggcaaaa ctattcacca agtgttagag atgacggtgg aagaggcgag
     3421 agaatttttt gcccctgtgc cgatgattgc ccgcaagttg caaaccttga tggacgtggg
     3481 gttatcttat atccgtttag ggcaatcttc tactacgctc tcaggcggtg aagcccaacg
     3541 ggtgaaattg gcgaccgagc tttccaaacg agacacaggc aaaacgctct atattttaga
     3601 tgaaccgacc accggcttgc attttgccga tattaaacag ttactcggcg tgctacatcg
     3661 cctgcgtgat caaggcaata cgattgtggt gatcgaacat aatttagatg tgattaaaac
     3721 cgcagactgg attgtcgatc ttggccctga aggtggcagt ggcggcggtg aaattattgc
     3781 taccggcaca ccggaagaag tggcaaaaga caaacgttct cacaccgcac gcttcttaaa
     3841 agagattttg gctaaaggct aacgttcaac acaagcggtc atattttcgg aaaatgttgc
     3901 aatttgcaaa aagttggaaa atatgaccgc ttgttttata tccggagtta ttcctagtta
     3961 gcctgtctga ttttgtcgaa ggcagattca agatcgctac tgaattgaat ttgcgggatt
     4021 tcgtttaccg ttttttgtga gcggactagc attctcatcg gctgaaattg cacattacag
     4081 agatgaagtt gttgatgtgg caacatatgc tgtacaaagc gggttagggc gtgaatgcca
     4141 ccagcatcta atactgtaac ggcatcgcat tgcagcacaa tatgtttaat ttggtgatcg
     4201 gtatgtacgg ttttattgtg cagatcggca aagagattat cggcggcagc aaaaaataac
     4261 ggaccgctga tccgataaac caacacatcg ttcaaatcgg ttgggtgagc catttcaaag
     4321 gatttagtca tttctgcaat agtacggata aatagcaggc ttgccagtaa cacccctacc
     4381 gtaatggcaa ttaccatatc aaataatacc gtgagggtaa tgcaagtcag taaaaccgca
     4441 atttcatttt tacctgaacg gcgggtaagt tgaatgattt gtggaatatc tgccatattc
     4501 caagctacta ccaataatag tgcagccatt gaggaaagag gcaaatagga aagtgcttta
     4561 gccaaaaaga gtagggcaaa taaaaccaac aaggcgtgaa ccacactaga aatgggggaa
     4621 actgccccgg aacgaacatt ggcagccgat ctcgcaatcg ctgctgtggc ggtaattccc
     4681 cctaaaaatg gcgaggcaat attacctaag ccttgggcta atagttcgtt gttagagtgg
     4741 tgcttggtat ctgtcatatt atcgcacact accgcacata ataacgattc gatagcccca
     4801 agcattgcca ttgaaaaggc agccggtaat aaggcttgaa tggtggttaa gttccaatct
     4861 atccgattcc cctctgcatt cgggagattc caaggtaaca caaattcagg taatacgctt
     4921 gggataccat acccagtggt gccgtcagag agcgtgtagt ggaatgctga gccgatagtg
     4981 gcaacgtttt gtccgaaatg aatcagcagt aatgataaca gcgtgccgat aaagacggcg
     5041 ggtaaatgcc cgggaatagg caaccgtagc ttgtgccaac gagttaaaac caacaatgtt
     5101 acgacaccca ctaaggtgtc agcccaattt atggttggca atgctgaaaa aatagcctct
     5161 agcttgccaa gatagtgttc aggcattttt tcaatgctta agcccagaaa atctttgatt
     5221 tgcaaggtgg cgatcacaat accgatgcca caggtaaaac ccagtgtgac aggtagagga
     5281 atgtattcaa ttaagcgtcc taatcggagt aacgccatca atactaaaat tcctccggaa
     5341 agcattgttg ccatgagtaa tccacttaag ccaaactgct gcgtgacggg gtatagaatc
     5401 accacaaaag cggctgttgg gcctgaaata ttaaatcttg aaccgcctgt tagggcaatc
     5461 acaatcccgg caacaatcgc cgtatataaa ccgtgctgtg gtggcacacc gctggcaatc
     5521 gccaacgcca tagataacgg aatagcaata acgccaacgg ttaagcctga aataatatct
     5581 ctgattaaat gttgtttacc ataacctgca ttgagagcat cttttaatgc actaaaaggc
     5641 ttgacggcaa gaaaagcatt tttagttaaa aaccatttat ttaacataga atgttcgagt
     5701 gaagagaatg tttcgcttat tttactgcaa tgaataaaat taaatttgag ctaacgcaag
     5761 aaaaagcagg aaagacgaaa tatgttggag acaagcggtt gttttaggcg aaaaatttgc
     5821 aaatacaaat aattagctga ttttattttg aaaatagcgt aaaatgccac tttaatttgt
     5881 actactatag tagtgcattt gtgtgatttc attaactata ataaacaaat ggacagtctt
     5941 attcattttt tgagtgcctt tttaactaat atcacttgga ttggggcgtt agagctgggg
     6001 ttaatttatt cccttgttgc ccttggggta ttaatttctt ataaaattct ggattttccg
     6061 gatctgaccg cagacggcag tttcccttta ggaggggcgg tgtgtgtgat cagcattttg
     6121 aatcaagttg atccttggct ggcaacttta tacggcacgc ttgccggagc agtagccggt
     6181 atggttactg caagtttgca tatcggcttt aaaatcgaaa aattgcttgc cagtatttta
     6241 atgatgatcg cactctattc gattaatcta cgcattatgg gaatgccgaa tattgctatt
     6301 ttaggcgaaa ccacgattta cgatgtaatg ccgattgaaa gcgatctaca gttagcctta
     6361 gtacgattag tgatgacatt gctggttgtt attatcgtaa aaggtttgtt tgacctcttt
     6421 tttgcgaccg aaatcggttt ggctgtgcgg gcaaccggta ctaatagccg aatggcgaaa
     6481 gcacagggta ttgcgattaa taaaatgacg attttaggga tggcgatttc taatgcgtta
     6541 attgccttag gtggtgcttt gtatgtgcaa agcaacggtg gtgtagatat ttcgattggg
     6601 gtaggaacta tcgtgattgg cttggcggcg gtaattatcg gggaagcaat tttttcggta
     6661 aaacggatta tttggcttac ctttgcggtg attattggct caattcttta tcgtttcttc
     6721 atcgcactag cattaaataa cgaaacttta aacggcattg gctttggccc gcaagatctg
     6781 aatttaatta cggcgttatt ggtggtgatt gtattagtgg ttcctcgaat taaacacaaa
     6841 ttgctcggta agcgaggtta atatgattga actcaataac ttatttatta ccttcaacaa
     6901 agggacagca attgagaacc ctgtgctgag aggcttatcc ctaaaagttg agcaaggcga
     6961 atttgtgtcg gtaattggta gcaatggggc gggtaaatct accttattaa atgcgatcag
     7021 tggtgattgt gcggtggact ccggtgagat tttaattcaa ggaaataagg taaaccaatc
     7081 caccacttgg caacgggcaa atcaagtcgc ccgagttttc caagacccga tggctggcac
     7141 ttgtgaaagt ttaacgattg aagaaaatat ggcactggct taccaacgtg gacgtaaacg
     7201 tgggttgggc tttgcgatca aacgccaaat gcgagagtta ttcaaagaca aacttgcttt
     7261 gttagggcta ggtttggaaa atcggcttac tgatcaaatg gggcgtttat caggcgggca
     7321 acgccaagcg gtgagcttgc tgatggcatc acttcagccg tctaatgtct tattgttaga
     7381 tgaacacacc gcggcactag atccgaaaac caccgatttt gtgcttgaac ttaccaataa
     7441 aattgtaaga gaacagaaat taaccacgct aatggtaacg cattcaatgc ggcaagccct
     7501 tgactatggc gatagaaccg tgatgttaca ccaagggcaa gtggcgtttg atgtttcggg
     7561 cgagcaacgg caaaaaatgg acgtgccgga tctgttacaa cttttccaac aaagtcgcca
     7621 cgagcagttg agtgacgatg gattattact cggcaattaa ttttgcgatc tgcatcgaaa
     7681 aaatgaattt ctccttttgt ttttggctgt caaattttaa ataataagcc caaaaggagg
     7741 tattcaatgt taagcatttt caaaacaccg gtagagaaag aaacgctaga cgattgggct
     7801 aaaatttcgg ttgatgtagc gaaagttgcg attttggcaa ttcctgttat actttttggc
     7861 aaagaaccaa cgttgattaa attgactaac ttagcattat tggtattgag tatttatgtt
     7921 tttttgacgc ttgggcgaaa attacggcag ctaaaggagg tgttgtgatg gaactaggct
     7981 ggaatttaac cataggtttg ctaattttaa acgtctttgc gtttgcttgg ctttatatta
     8041 ttaaccggat ttctaaagaa caattatcca aaaatcaata atatttaatt tttcacaagc
     8101 ggttgttttt gcaaaatatt ttgcaaattc gaccgcttgc ctgtttttaa atgacaaaat
     8161 acgacgataa aattcagcac gcatttagcg aaaatgggtt gctcagccaa aatatcagtg
     8221 gatttcgacc acgccaagca cagcttgaaa tggctcaagt ggtcgcaaaa gcagtaaaat
     8281 ttgcgacacc tgttgtggta gaggcgggaa ccggcacagg caaaaccttt gcttatcttg
     8341 tgccggcact actttcaggc aagaaaacta ttctttcgac aggttctaaa aacctgcaag
     8401 atcaactttt taatcgagat cttcccacta ttcaaaaagc cctgaaatac aaaggcaaaa
     8461 ttgcgttact caaagggcgt gcgaactatt tgtgtttaga gcgtttagat caagtgaccg
     8521 caatgggcgt acttggggat aaatccgttc ttaccgattt aaaaaaagtc agtaaatggc
     8581 aaaatgccac taaaacaggg gatttaagtg aatgtattac cattgccgaa gatagcccga
     8641 ttctaccgca gttagtcagc accaccgaga gttgcttagg ctcggactgc ccgcatttta
     8701 aagattgtta tgtggtacaa gcccgccgta aagcgatgga ggcagacgta gtggtggtaa
     8761 accatcacct attctgtgct gatatggcag taaaagagac cggttttggt gagttgatcc
     8821 ctgaagctga gctggtgatc tttgatgaag ctcaccaact gccggatatt gccagccaat
     8881 attttgggca atcgctttct tcccgccagt tatttgatat ttgtaaggat accaatattg
     8941 tttaccgcac tgaactcaaa gatctgtctc aattaggcaa agcggctgat cacttgacga
     9001 aagtagtgca agatttccga ttattgatgg gcggagaagg gcagattcga ggcaatttgc
     9061 gagagttatt tcgtgataaa aaagtggtcg atggcttggt aaaagtggct gaaaatattg
     9121 attttttaag tgaagtggcg aaaaaatcgc tggggcggtc ggaaatgttg gataaaattt
     9181 tcgagcggtt agcagaagtt aaggttcaac ttaaaaaatt aatggatact agtgtgacag
     9241 gctattgtta ctggtatgag gcgaatggac gatcctttgg gctgcatatt acgccgctta
     9301 tggtggcaga taaatttgga gaacagttga agaaccagca aattggttgg gtgtttacct
     9361 cagccacact cgaagttggg ggcaaattcg atcatttttg tcaacggctg ggcatcgaaa
     9421 atgcggagca gttggtgtta caaagtccgt ttgattacca acatcaatct ttactctgtg
     9481 tgccccgcta cttgccggat accaataaat ccaatacttt aactgctctc gggcaattat
     9541 taaaaccggt gattgaggcg aataaagggc gttgtttctt gctttgtact tcctattata
     9601 tgatgcgagg tttagccgac tttttacgcg aacacagttc gctgaatgtg ttattgcaag
     9661 gcgaaaccag caaaagccgt ttattggaaa aatttgtgaa agaagatcac agcgtgttgg
     9721 tggcgaccca aagtttttgg gaggggattg atgtgcgagg cgatgattta tcgttggtga
     9781 ttattgataa attgccgttc acttcaccgg acgaaccatt actcaaagcc cgaatggaag
     9841 attgtaagat acagggcggt gatccgttta acgatattca aattcccgaa gcggtgatta
     9901 ccctcaaaca aggtgtaggg cgtttaattc gtgatgtgac cgatagaggt gtagtgatta
     9961 tttgtgattc ccgtttggtg atgcgaaatt atgggcaaac tttcttgaaa agtttaccgc
    10021 cgtctagacg cactagagat ttggggaatg ttattcaatt tttgaaacaa aaatagggtt
    10081 acaagcggtc agatttctgc agatttttgc aaaaaaacgg ataaaaataa ccgcttgttg
    10141 aggagttata tgttgtctac attgctgcgt atggttgctt ctttggcatt gttatttgtg
    10201 atgctttatt taggcaagtt aatcgtttat ctattcccca tcgggatccc ggatagtatt
    10261 ttaggtatgc ttatcttatt cggctgcttg gttatcggca ttgttaaagt ggaatgggtg
    10321 ataccgagcg cgaaaccgct cacacgttat ataacactat ttttccttcc gatttgtgtc
    10381 gaactcatcg aacatcttga gctgttacgc caaaatctga acacttttgt attagcaacg
    10441 gcattaagca ccttagtttc tgtggtcgcg attggcgtgt ttgcacagtg gatttttcat
    10501 tacaagagag ggtaaaatga gctatcttta cactgtgttg acggtaatca tgtttattgc
    10561 cggtttgaaa atcagccaaa aactcaagag cagttttctg aaccctttct taattgcact
    10621 cgctttgcta attggtgtat tactgatttt taatatccct taccaagact attatcaagg
    10681 taattccccc ttgaataact tacttggcgt ttctgttgtg gcacttgctt acccttttta
    10741 tgaacaatta ccccatatcc gtaaaaaatg gcgacagatt gtcgtgattt tgctgtttag
    10801 taccatcatt acgatgataa ccgggcttgg gttttcatta ttatttgggg caagccaaga
    10861 gatcttagcc gccattttgc ctaaatcggt atcaatgcca atggcaatta ttattacgaa
    10921 tgaaattaat ggaaatgttg caatcgcagc ggttggggtg atgatcgcag gcttagtggg
    10981 atctatttta ggctttacgc ttctgcgcct ggcaaaagta agaaatgtga tggcgattgg
    11041 gttaagtatg ggagctgctt cccacgcatt aggcacggca agatgcttag agcatagtat
    11101 gaaagccggt agctatagtt ctatcgcatt agtggcttgt ggtgtgctgt cttctatatt
    11161 tgcaccttta atatttaacg ttatcgtcac gttatgttac taaaaacagt gaaatctcaa
    11221 accttatttg gtaactggct gaaatcaagg tacaataccg tgatttttca atgaaaatag
    11281 agaattatgc aagaacaaat aacacaagaa aaagatcttt ctacgcttaa aacctttaag
    11341 cgtttatggc cgactatcag cccgtttaaa gcgggtctga ttacaggtgc tgtcgcttta
    11401 gtattaaatg cgttagtgga ttctagtctt atctatctcc tcaaaccctt gctagatgaa
    11461 ggctttgggc aagctaataa tgattttctg aaattgatgg cggtgcttgt agtagttttt
    11521 attttagtgc gcggtattac caattatatt gccagttact gtttagcttg ggtgtcaggc
    11581 aaggtagtaa tgacattacg ccgccatatt tttcaacatt taatgtatat gccggtaagc
    11641 tattttgata acaacccgac cgggcgtttg ctctcacgag tgacttacga taccgaaatg
    11701 gtggcgagtt ctgcttctca ttctttagtg actattgtgc gtgaaggggc ttatctgctg
    11761 tcattgtttg tggtgatgat ctataccagt tggcagcttt ctattgtgtt atttgtgatg
    11821 gctcctatca ttggggtact gatcagtttc gtttcaaaat attttcgtca attaagtcgc
    11881 aatatccaaa gttcaatggg ggagcttact gtcacgacag aacaaatgct taaagggcat
    11941 aaggtggtac tttcctttgg cgggcaggaa attgagaaag ctcgttttga ccgagtcagt
    12001 aatgatatgc ggcgtaaagg tatgaaagtc gtttctgctg atagtatttc tgacggtgtg
    12061 gtgcaactta ttgcctcttt tgcgttatct gcggtgcttt acgttgcgac cttccctgaa
    12121 gtgatgagtg agaatttaac cgcaggttca ttcacagtag tgttttcttc gatgatggca
    12181 atgttacgcc ctctaaaatc actcaccaat gtgaactcac aattccaacg tggaatggca
    12241 gcctgccaaa cattatttga atttttagat ttaaaaactg aaaaagatca cggctcaatc
    12301 tctgttgtta aagcagaagg caatgtggaa tttaaaaacg tgagctttag ttatgaaggc
    12361 aaagatgaaa aagcattaaa taatatttct ttcaccattc ctaaaggcaa aacagtagca
    12421 ttggttgggc gttccggctc gggtaaatca accattgcca atttgcttac ccgtttttat
    12481 gatgtgactg acggtgaaat tttgcttgat ggcattaaca ttcaagatta ccgcttaaaa
    12541 aatttaagag aacaatgttc agtggtttcg cagcaggtgc atttatttaa cgacactgtt
    12601 gccaacaata tagcctatgc tgccaccgat aaatacactc gagaagaaat tattgtcgct
    12661 gcaaaagctg cacacgcaat ggaatttatc gaaaaactgg aaaatggctt agatacagtg
    12721 atcggtgaaa acggagcaag tctttccggt gggcaacgcc aacgcttggc aatcgctcgt
    12781 gcattactgc gaaattctcc tgtgcttgtg ctggatgaag ccacctctgc attagatacg
    12841 gagtcagaaa gagcgatcca atctgcactt gaagtgctac agaaagatcg tacggtgctg
    12901 gttatagcac accggttatc cacgattgaa aaagccgatg aaattttggt agtcgatcac
    12961 ggaaatatta tcgagcgtgg cagccatacg gaattgttag cacaaggtgg ggcttataaa
    13021 caactccata gtttacaatt cggagcctaa tttgtagggg agaaatatgt ttctccccaa
    13081 ttaatgataa aaacagggcg aaaaatattt cgtccctaca ataatttatt tggatactat
    13141 gaaaaaactg acaaaacttt tatctcttag tgctttactg gtcttaaccg cctgtaattc
    13201 ggtactaaat gccccaacag aggtgcaaca accaacagta caaattccgc ataacgatcc
    13261 acaatggcag cagcatttag cgaaactgag ccaaattcaa ggctatactg caacggggca
    13321 atttggctat atttcgcctg aagagcgttt ttcttcccat ttcaactggc gttatactaa
    13381 tccaatccaa tttgggttag agctttcctc gaatctctcg actaaatcac tgaaattaga
    13441 gcgtaataaa cgaggcttaa cggtttcgga cagtgagggt aatagccgtt ctgaccgtga
    13501 tgttgatgcg ttaatgcaag agattatcag tgtttcgttt cctatcgatc agtttgccta
    13561 ttgggtaaaa ggcttgcctg aaaaagatgg caactatatt gtgaatgaaa aacgccagct
    13621 gtcgcaattt agttatccgc ttaatggcac gctttggaaa gcaagctatg tggagtatca
    13681 cgaagatcgt gtaccaaact taccgaagct gattgtgctt gaaaacggca ctcaaacgtt
    13741 gaagatccgc attgaaaaat gggcgtatta atgaagaaag tcaccttacc cagcccggca
    13801 aaattaaacc tgtttttata cattaccaat aagcgggcag acggttatca cgagctacaa
    13861 acgctgtttc agtttttaga ttttggtgat gaaattgaga ttgagatcac agaaagttca
    13921 gaggttgaat taattaatcc ggtagaaggg gtggcaactg aggagaatct catttaccga
    13981 gcagcaaaac ttttgcaaaa aacgaccgct tgtaggcaag gctgccgtat tgggattaaa
    14041 aaaaacctac cgatgggcgg aggtgttggt ggtggatctt ctaatgctgc aacagtatta
    14101 gtcggtttaa atcacttgtg gaataccgag ctttcattgg aaaaattggc agaattaggc
    14161 ttaagtttag gggctgatgt gccgatattt gttagaggta tggctgcttt tgccgaaggc
    14221 gtgggggagg tgttaacccc ttgtgatcca caagaaaaat ggtatgtagt gctaaaacca
    14281 gaagtgtcga tttcaaccga agcggtgttt accgacccga atttacctag aaatactcca
    14341 aaacgcccct tagccgaatt attatcttta ccgtgggaaa acgattgcga aaaagttgta
    14401 cgagaaaagt attctgcggt tgaagatctt atacaagaat tgttacaata tgctcagttt
    14461 aggctaacag gcacaggtgc ttgtattttc gccgaatttg aaacaaaagc agaagccgag
    14521 caggtttttg cacataaacc ggaagccgtc ttcggttttg tggcgaaagg gcaaaatatt
    14581 tcgccattac atcaaatgtt attccaaatc taaccatctt aaccttaagg atcaatccat
    14641 gacagatatt aaactgtttg cgggcaacgc aactccggaa cttgctaatc gtattgcaaa
    14701 acatctttat acctcattag gtaatgcaac agttggtcgt tttagcgatg gtgaaattca
    14761 agtacaaatc aatgaaaatg tgcgtggagg cgatattttt attgtgcaat ccacttgtgc
    14821 accaactaat gataatttaa tggaattgat cgtgatggta gatgcgcttc gccgtgcatc
    14881 agctggtcgt attaccgcag tgattccgta tttcggttat gcacgccaag acagacgcgt
    14941 gcgttcagcc cgtgtgccaa ttaccgcaaa agtgattgct gatttcttat ccagcgtagg
    15001 ggtagataga gtcttaacct gcgacttaca cgcagagcaa attcaaggtt tctttgatgt
    15061 gccggtagat aacgtatttg gttcccctgt gttaatcgat gatattttga aaaaatccga
    15121 tttggtcaac cctatcgtgg tttctccaga tattggtggg gttgttcgtg caagagcggt
    15181 ggcaaaatta ttaaatgata ccgatatggc aattattgat aaacgtcgtc caaaagcgaa
    15241 tgtatcacag gtaatgcaca ttatcggtga tgtggctgat cgtgactgta ttttagttga
    15301 tgatatgatc gatactggtg gtactttagt caaagcggct gaagcgctga aagaacgtgg
    15361 cgcccgtcgt gtctttgctt atgccacaca cgcggtattt tcaggctcag cagctcaaaa
    15421 tttagcgaat cctgctttag atgaaattgt agtaactgat actattccac tgagtaatga
    15481 gattaaagca ttagataaag tacgtgtttt aacattatcc ggtatgttag cagaagcaat
    15541 tcgccgtatt agcaatgaag aatccatttc agcaatgttt gatgcctaat ttatgagata
    15601 aaaacccagt cgaaagattg ggtttttcat atctgttcac tactatattt agtttaagat
    15661 aatttgtatg tattccctga ttcgtaaatt actttttgca ctagatacgg aaaaagcaca
    15721 tcaattctct attcaggcac ttgctttagc cggaaaatcg cctttttcga ttctgccaac
    15781 acccgataat ccgacccaag taatgggact cacgtttaaa aatccaatag ggcttgcagc
    15841 cggagcggat aaaaacggtg aagcgataga tggttttggg aaattaggat ttgggtttat
    15901 tgaggttggg actattactc ctctcgctca agacggaaac cctaaaccga ggcaatttcg
    15961 tattgtagat gcagagggaa ttattaaccg caatggattt aataataaag gcgttgatgt
    16021 tttagtagaa aatgtaaaaa atgcaaccta tagtggtatt ttaggtatta atattggaaa
    16081 aaatgcggtt acaccaattg agcatagctt tgatgactac caaatttgtt tgcgtaaggt
    16141 ttaccaatat gctgattatg tggcggtgaa tatttcatca cccaatacca aaaatttgcg
    16201 cagtttacaa tatggtgagg cattagatga tttgctgcga aacctcaaaa atgagcaggc
    16261 acaactttcg caaaaaatgg ggaaatatcg accgcttgtt ttaaagattg cgcctgattt
    16321 aaccgatgaa gaaatcgttt ctgtagccga tagtcttatt cgttaccaaa ttgatggggt
    16381 gattgccggc aatactacct tgtctcgtga aagtattgcc ggattaccgt attcggaaca
    16441 actgggtgga ttaagtggca agccattaca ctcattaagt acaaggttaa tttcaaaatt
    16501 atctgccgag ttgggcgaga gaataccaat tataggttgt gggggagtcc attcgttaga
    16561 gagtgggcag gagaaagtga atgcaggtgc tagtttattg caagtctatt ccgcaatgat
    16621 ttaccaagga ccatcattaa ttcaaacctt agccaaacac cttcagctga acagttaggg
    16681 atagatttct agttaaaaat gattggttag gtttatttct gccatcttag tggaatagat
    16741 ggcggtatta gct
//
LOCUS       DANJWC010000041        16732 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.041, whole genome shotgun sequence.
ACCESSION   DANJWC010000041 DANJWC010000000
VERSION     DANJWC010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16732
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.041"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..1578
                     /gene="argS"
                     /locus_tag="PWX02_000964"
     CDS             <1..1578
                     /gene="argS"
                     /locus_tag="PWX02_000964"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDL1728993.1"
                     /translation="LGMNPREFAQKILENADLNGIADKLEIAGPGFINIFLNPEWLAE
                     NTNSSLQAVNFGIKTANPQTIVIDYSSPNVAKEMHVGHLRSTIIGDAVVRTLAFLGNN
                     VIRANHVGDWGTQFGMLIAYLEKMENEHVSEMELSDLEAFYRAAKEHYDSDEAFAEKA
                     RNYVVKLQSSDKYCLTMWKKLVDITMHHNQENYNRLNVTLTEKDVMGESLYNPMLPEI
                     VADLKAKGLAVEDDGALVVFLDEFKNKDGDPMGVIVQKKDGGFLYTTTDIAAAKYRYE
                     TLKADRALVFSDSRQAQHMQQAWLITRKAGYVPDSFSLEHPFFGMMLGKDGKPFKTRS
                     GGTVKLKDLLDEAVERADKLIAERSTELTAEEKAAVVEAVAIGSVKYSDLSKNRTTDY
                     VFDWDNMLTFEGNTAPYMQYAYTRIRSIFARANIDEDALSGSIQLNEPKERALAVKLL
                     QFEEALNGVAKEGMPHILCQYLYELAGSFSSFYEACPILNAEETTKNSRLKLAALTAK
                     TLKQGLDLLGIKTVEKM"
     gene            1768..2934
                     /gene="cgtA"
                     /locus_tag="PWX02_000965"
     CDS             1768..2934
                     /gene="cgtA"
                     /locus_tag="PWX02_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HDL1728994.1"
                     /translation="MKFIDEALIRVEAGDGGNGCVSFRREKFIPKGGPDGGDGGDGGD
                     VYLIADENLNTLIDYRFEKRYAAGRGENGRSAGCTGHRGQDITLRVPVGTRAIDNDTQ
                     EIIGDLTRHGMKMLVAKGGYHGLGNTRFKSSVNRAPRQKTNGTPGEKRDLKLELLLLA
                     DVGMLGLPNAGKSTFIRSVSAAKPKVADYPFTTLVPSLGVARVGADRSFVVADIPGLI
                     EGAADGAGLGIRFLKHLERCRILIHLVDIMPIDESDPAHNISVIESELYQYSEKLAEK
                     PIWLVFNKIDTIGEEEAAERAKDIAEQIGWEGDYYLISAATGQNVQALTRDIMDFIEA
                     NPREVEEENKAPEEVKFKWEDYHNEAMQNAVEEDWDDDWSEEDDEGIEFLYERR"
     gene            3019..3636
                     /locus_tag="PWX02_000966"
     CDS             3019..3636
                     /locus_tag="PWX02_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HDL1728995.1"
                     /translation="MLSSTSCDLFNIPFFQFAQMKKYCPEEIPRIKAEYKTQWEIWKK
                     LHQEVSRQLGQPFAPPHIEKWCNGWQVRAHFFAYYKYEFNRNSAAILSVILNRRRLQV
                     SLDWHCYRADRSQINVHQYNQWWENLDREKFADFEIWHGSESEYDDFRTVSQMSEQAF
                     TLTNEEDFWCIGKNVEKDDLDNIDVVEFIYQTIRALLPLYEKCHQ"
     gene            complement(3683..5641)
                     /locus_tag="PWX02_000967"
     CDS             complement(3683..5641)
                     /locus_tag="PWX02_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HDL1728996.1"
                     /translation="MIAELGNYTLALALAISILLAVLPLWGAEKQNSTLIALARPMTW
                     AMFLALTVSFGSLFYLFAVNDFTVQYVVNNSNSRLPLQYRLSAVWGAHEGSLLLWVWL
                     LSLWSVGVATFTRKMPEDAVARVLGLMGVIGIGFLMFVIFTSNPFDRTFPNFPIDGKE
                     LNPMLQDVGLIFHPPLLYMGYVGFSVAFAFAIASLMTGRLDTAWARWSRPWTMAAWVF
                     LTLGIVLGSWWAYYELGWGGWWFWDPVENASLMPWLAGTALIHSLAVTEKRGTFKAWT
                     VLLAILAFSLCLVGTFLVRSGILVSVHAFASDPTRGLYILAYLVLVVGGSLLLYAFKG
                     SEIKSLDNYERYSRETMLLINNILLMAFLSVVFLGTLLPLIHKQIGLGTISIGAPFFD
                     QMFLILMVPFSFVLGIGPLVKWRRDQVSAIRKPVIIATILMALLGFGLPYLFRNTITV
                     SVVLGTMMSVFIVLLSLYELHQRATHRHSFLSGIFKLSRSHWGMVLAHLGVAMTVFGI
                     AFSQNFSIERDVRMNVGDKAEILDYQFEFKGIKITDGANYQGGTAELEITRNGKYEAT
                     LNAEKRFYTVSRMGMTEAAIDWGFTRDLYAALGEKLEDNSWAVRLYYKPFIRWIWIGG
                     LFMALGGLLCMLDKRYRLRIENKKEVIA"
     gene            complement(5638..6195)
                     /gene="ccmE"
                     /locus_tag="PWX02_000968"
     CDS             complement(5638..6195)
                     /gene="ccmE"
                     /locus_tag="PWX02_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HDL1728997.1"
                     /translation="MNPRRKSRLKVVVSILLGLSVAAGLTLYALSQNIDLFYTPSEIV
                     NGKNDDPKQKPEVGQRIRVGGMVVEDTVKRDDKSLKVVFDLNDIGPSITVEYEGILPD
                     LFREGQGIVAQGVLIELTRLKATEVLAKHDENYMPPELGDKLKEQHKKSGVSEQDLKG
                     ESETDRKMKAEAEAEKANNVQGESK"
     gene            complement(6381..6578)
                     /gene="ccmD"
                     /locus_tag="PWX02_000969"
     CDS             complement(6381..6578)
                     /gene="ccmD"
                     /locus_tag="PWX02_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HDL1728998.1"
                     /translation="MTFQFESISDFFAMGGYGFYVWLSYGVSFIAMIGLIWLSRREQK
                     AILAQAKKEIVREEKLKQTSQ"
     gene            complement(6593..7330)
                     /locus_tag="PWX02_000970"
     CDS             complement(6593..7330)
                     /locus_tag="PWX02_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HDL1728999.1"
                     /translation="MWKWLHPYAKSETQYHLLGKIQPFLGWLSLIMLAAAFVWGLAFA
                     PKDYQQGDSYRIIFIHVPAAIWSMGVYGSMAVAGLVALVWQIRQANLAMISMAPIGLV
                     FAFISLATGAIWGKPMWGTWWVWDARLTSALVLFFLYIGVMALYSAFQDRQTGAKAAA
                     VLSVVGVINLPIIHFSVEWWNTLHQGATITKFDKPSMAVEMLIPLLLAIFGSLIFTAW
                     FSIWRYRIALLKDERNRPWVKQLAKSV"
     gene            complement(7530..8192)
                     /gene="ccmB"
                     /locus_tag="PWX02_000971"
     CDS             complement(7530..8192)
                     /gene="ccmB"
                     /locus_tag="PWX02_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005565617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HDL1729000.1"
                     /translation="MILLTIIQRELTIAFRKPSEILNPLWFFLMVITVFPILMGPNPE
                     LLGKIAGGIAWVAALLSALLSFERLFRDDYLDGSLEQLMLLPLALPQVALAKVIAHWL
                     LTGLPLILLSPIAAILLSLETHVWWALVLTLLLGTPILSCLGAIGVALTVGLRKGGTL
                     LSLLILPLFLPVLIFAAAVLEAATLNMGYSGQLAIIGAILAVTLTFSPFAIAGALRIS
                     LN"
     gene            complement(8189..8830)
                     /gene="ccmA"
                     /locus_tag="PWX02_000972"
     CDS             complement(8189..8830)
                     /gene="ccmA"
                     /locus_tag="PWX02_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HDL1729001.1"
                     /translation="MNQTAQYQLKLNQIACERGETRLFEGCSFTVQSGEWVQIEGHNG
                     IGKTSLLRILAGLSLPAEGEVLWNDIAIQKQRDHYYSELFYLGHYSGVKPELTPWENL
                     RFYQKIQQLPQDDDALWHALTKVSLIGREDLPCSHLSAGQQRRVALAKLWLTNAKLWI
                     LDEPFTAIDKKGVADLIAHIEQHCEQGRLVIFTSHQAAESDKVRIISLDQFKV"
     gene            complement(8974..9684)
                     /gene="hda"
                     /locus_tag="PWX02_000973"
     CDS             complement(8974..9684)
                     /gene="hda"
                     /locus_tag="PWX02_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HDL1729002.1"
                     /translation="MQLPLPIHQIDEDTFDNFYSQNSEVLLESLKRNFTDVQQPFFYI
                     WGGKSCGKSHLLKAVSNHFLLNNQTSSYIPLTKANYFSPLVLDNAELLNVICLDDIQA
                     IAGDAEWELAIFNLFNQIREQQGLFNEGQKTLLLISADCPPHQLNIQLPDLRSRLTWG
                     EVYQLNDLTDEQKCQILQLNAHQKGVELSDEVAHFLLKKAGTDLEQLSKILDKLDKAS
                     LQAQRKLTIPFVKEILSL"
     gene            9954..11555
                     /locus_tag="PWX02_000974"
     CDS             9954..11555
                     /locus_tag="PWX02_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1729003.1"
                     /translation="MKNMTKLSLITLALAASSSVFAAPKTFVYCLEASPSALSPVLST
                     DGASFDVNALPIYNKLTDFEDGTTNVIPSLAEKWDISEDGKTYTFHLRKGVKFHDNKE
                     FKPSRELNADDVVFSINRQFDPNHPFHKVSGGNYEYFIGMDMQNIIDKVEKVDDHTVK
                     ISLKVPNAPFLANLAMDFNAVYSAEYAEQSLKAGKPERIDTNPIGSGPFQFVDYQKDA
                     TVRYKAFENYWQGKAKIDRLVFAITPDASVRLAKLQKGECHAMPYPNPADIENLKKDP
                     NIELMSQSGMNIGYLALNSSIKPLDNQKVRQALNHAVNKQAIVDAVYQGAGQVAKNPI
                     PPTMWSYNEAVQDYDYNPEKAKALLKEAGFENGFDTELWAMPVSRPYNPNARRMAELI
                     QEDWKKVGVNAKIVSYEWGEYLKRLRQGEAATSMIGWTGDNGDPDNFLNTLLSCSAVE
                     AGSNYAKFCHKPFEEVVLGAAQESDKAKRTELYKQAQVIFKEQAPWITIAHSTVYFPV
                     RKEVKGYVMSPFSLHNFYKVDLADK"
     gene            11827..12831
                     /locus_tag="PWX02_000975"
     CDS             11827..12831
                     /locus_tag="PWX02_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL1729004.1"
                     /translation="MFSFILKRVLMVIPTFIAITLITFALVHFIPGDPIEIRMGERGL
                     SPEVHQQMMEQLGLNRPLFEQYISYIKGVLHGDLGNSFRNNEPVLKEFFTLFPATVEL
                     AFFALLWSLIFGIILGVIAAVKKESWISHTVTTLSLTGYSMPIFWWGLILILYFSTPL
                     GLPASGRLPSEYWIEAETGFMLIDTWNSDEPGAFLAAIKSLILPSIVLGTIPLAVVTR
                     MTRSSMLEVLGEDYIRTAKAKGLNTTRIVIVHALRNALIPVVTVVGLIVGQLLSGAVL
                     TENIFSWPGIGKWIIDAINSRDYPVLQGSVLIIATIIILVNLTVDLLYGIVNPRIRHS
                     "
     gene            12933..13826
                     /locus_tag="PWX02_000976"
     CDS             12933..13826
                     /locus_tag="PWX02_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL1729005.1"
                     /translation="MSGITAVNPPAPKTPLQEFWYYFCQNRGALIGLTFIAIVFVACV
                     FAPWVAPFDPIEQNRSALLLPPAWLEGGNSAHLLGTDDIGRDILSRIIYGSRLSVFIG
                     LVIVLLSCVFGVILGLLAGYYGGTLDIIIMRFVDIMLAIPSLLLTIGVVTILGPSLIN
                     AAIAIAIVSIPSYVRLTRASVLSEKNRDYVTASRVAGASVLRLMFVVILPNCLAPLIV
                     QMTMGISNAILELAALGFLGIGAQPPTPELGTMLAESRGFMQSANWLVTIPGLAILSL
                     VLAFNLMGDGLRDALDPKLKQ"
     gene            13837..14826
                     /gene="dppD"
                     /locus_tag="PWX02_000977"
     CDS             13837..14826
                     /gene="dppD"
                     /locus_tag="PWX02_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDL1729006.1"
                     /translation="MALLTVDQLSVHFGETKTPFKAVDRISYEVNEGEVLGIVGESGS
                     GKSVSSLAIMGLIDFPGRVMAEKLYFNGNDLLALKPKEKQQIVGADVSMIFQDAMTSL
                     NPSYTVGYQIMEALKVHQGGSKASRRERAIELLTMVGIPDPESRLEVYPHQLSGGMSQ
                     RVMIAIAIACRPKLLIADEPTTALDVTIQAQIIDLLLELQRKENMALILITHDLALVA
                     ESAHRIIVMYAGQVVEEGKAEQIFKAPLHPYTQALLKALPEFAEGKSRLQSLPGVVPG
                     KYDRPQGCLLNPRCPYATEKCRTIEPAMQMINGRQVKCHTPLNAQGEPIRIAV"
     gene            14954..15943
                     /locus_tag="PWX02_000978"
     CDS             14954..15943
                     /locus_tag="PWX02_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1729007.1"
                     /translation="MKENNSLPPLLKAVDLKKYYPVKKGMFAKPKTVKALDGVSFTLE
                     RGKTLAIVGESGCGKSTLGRMLTMIESPTEGELFYNGQNFLVNDKETAQLRRKKIQIV
                     FQNPYSSLNPRKKVGSILEEPLLINTNLSAKERKEKVLEMMAKVGLKPEFYDRYPHMF
                     SGGQRQRIAIARGLMLQPDIVVADEPVSALDVSVRAQVLNLMMDLQEEMGLSYVFISH
                     DLSVVEHIAAEVMVMYLGRCVEQGDTKTIFSNPRHPYTQALLSATPRLNPEQRRERIK
                     LTGELPSPLNPPKGCAFNARCRFATDRCYVERPELKTYQDGTRMACFVVEENN"
     gene            complement(16181..16726)
                     /locus_tag="PWX02_000979"
                     /pseudo
     CDS             complement(16181..16726)
                     /locus_tag="PWX02_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005758802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         4990 a         3687 c         3542 g         4513 t
ORIGIN      
        1 ttggggatga atccacgtga atttgcccaa aaaatcttag agaatgccga tttgaacggt
       61 attgccgata aattagaaat tgccggtccg ggctttatca acattttctt aaacccagaa
      121 tggctggcgg agaacactaa cagttcattg caagcggtca attttggcat aaaaactgca
      181 aatccgcaga cgattgtgat cgactactcc tcgccaaacg tagcaaaaga aatgcacgtc
      241 gggcatttgc gttccaccat tattggcgat gcggtagttc gtacccttgc gtttttaggc
      301 aataatgtga ttcgtgccaa ccacgttggc gactggggta ctcaattcgg tatgctgatc
      361 gcttaccttg aaaaaatgga aaatgagcac gtcagcgaaa tggagctaag cgatttagaa
      421 gccttctatc gtgcggcaaa agagcattat gattcagacg aagcattcgc cgaaaaagcc
      481 cgtaactatg tggtgaaatt acaaagcagc gataaatatt gcttaacaat gtggaaaaaa
      541 ttggtcgata ttacaatgca ccacaatcaa gagaactaca atcgcttaaa tgttacgctc
      601 accgaaaaag atgtaatggg cgaaagcctc tacaatccaa tgctgcctga aattgtggcg
      661 gatttaaaag ccaaaggctt agcggtggaa gatgacggtg ctttagtggt tttcctcgat
      721 gaatttaaaa ataaagatgg cgacccaatg ggcgtaatcg tgcagaaaaa agatggcggt
      781 ttcttatata ccactaccga tattgcggca gcaaaatatc gctacgagac cttaaaagct
      841 gaccgagcct tagtcttttc tgacagccgc caagctcaac atatgcaaca ggcatggctc
      901 attacccgca aagcgggcta tgtgccggac agtttcagcc ttgaacaccc gttcttcgga
      961 atgatgctag gcaaagacgg caaaccgttc aaaacccgta gcggtggcac agtcaaactc
     1021 aaagatttat tagatgaagc ggtagagcgt gccgataaac tgattgccga acgttcaacc
     1081 gagctaactg cggaagaaaa agccgccgtg gttgaagcgg ttgcgattgg ttcggtgaaa
     1141 tattctgact tatcgaaaaa ccgcaccacc gattatgtgt tcgattggga caatatgctg
     1201 acctttgaag gcaataccgc cccgtatatg cagtatgcct acacccgcat tcgctccatc
     1261 tttgcccgtg cgaacattga cgaagatgca ttgagcggtt caatccaact caatgagcca
     1321 aaagagcgtg cattagcagt gaaactattg caatttgaag aggcattaaa cggcgtggca
     1381 aaagaaggta tgccacatat tttatgccaa tatttatacg agcttgcagg tagtttctcc
     1441 agtttctacg aggcttgccc gattctcaat gccgaagaaa caaccaaaaa cagccgtttg
     1501 aaactcgctg ccttaacggc gaaaaccttg aaacaagggt tggatttact cggtattaaa
     1561 actgtagaga agatgtaata caaactctct ccccttgtgg gagagagacc gcttatgagc
     1621 gttcagcgaa ataagcagag agagggtaca agcggtaatt tttattaaaa tttttacaca
     1681 ttttcccctc tccctgattt ttcttctgaa cgaagaaaaa tctgtccctc tcccacaagg
     1741 ggagagggca tataaaagga aacaaatatg aaatttattg acgaagccct gattcgtgta
     1801 gaagcaggcg atggcggtaa tggctgtgtg agcttccgcc gtgaaaaatt tatcccgaaa
     1861 ggcggacctg acggtggtga tggtggcgat ggtggtgatg tgtatttaat tgccgatgaa
     1921 aacctaaaca cccttatcga ctaccgcttt gaaaaacgtt atgctgctgg gcgtggcgag
     1981 aatggtcgca gtgccggctg taccggacat cgtgggcaag atattaccct acgagtgccg
     2041 gttggaaccc gtgcgattga taacgacacc caagaaatta ttggcgattt aacccgccac
     2101 ggaatgaaaa tgctggtggc aaaaggtggt tatcacggtt taggtaatac ccgctttaaa
     2161 tcctcagtaa accgagctcc acgccaaaaa accaacggta cgcctggcga aaaacgtgat
     2221 ttgaaattag agctgttact gcttgccgat gtgggaatgc ttggcttacc aaacgccggt
     2281 aaatccacct ttatccgcag tgtctctgct gccaaaccaa aagtggcgga ttacccgttc
     2341 acgacgctgg tgccaagttt aggggtagcc cgtgtggggg ccgaccgcag ttttgtggtt
     2401 gccgatattc cggggttaat tgaaggtgca gcagatggtg cagggctggg gattcgtttc
     2461 ctaaaacact tggaacgttg ccgtattttg attcacttag tcgatattat gccgattgat
     2521 gaaagcgatc cggctcataa tatttcggtg attgaatccg agctttacca atacagcgaa
     2581 aaactcgccg aaaaaccgat ctggttggtg tttaataaaa ttgacaccat cggcgaagaa
     2641 gaagctgctg agcgagcaaa agacattgcc gagcaaatcg gctgggaagg cgattactac
     2701 ttgatttctg ccgctaccgg tcaaaacgtg caagctctca ctcgtgatat tatggacttt
     2761 attgaagcca acccacgcga agtggaggaa gaaaataaag cacctgaaga agtcaaattc
     2821 aaatgggaag actaccacaa cgaagcaatg caaaatgcgg tagaagaaga ttgggacgac
     2881 gattggagcg aagaagatga cgaaggcata gagtttcttt acgaacgccg ttagtgcaat
     2941 aatacaagcg gttgttttta aacggagatt tgcaaatttc cggagaaaaa caaccgcttg
     3001 taggtttaag gagagtttat gcttagctca acatcgtgcg atttattcaa tattccgttc
     3061 ttccaatttg ctcagatgaa gaaatattgc cctgaggaga tccctcgaat taaggcggaa
     3121 tataaaactc aatgggaaat ttggaaaaag ctacatcagg aagtttctcg tcagcttggg
     3181 cagccgtttg ctccaccaca tattgagaag tggtgcaacg gttggcaagt tcgagcccat
     3241 ttttttgcct attacaagta tgaatttaat cgaaattctg cggctattct ttccgtgatt
     3301 ttaaatcgtc gccgtttgca agtgagcctt gattggcatt gttatcgagc cgatcgctcg
     3361 caaattaacg tgcatcaata caatcaatgg tgggaaaatt tagatcggga aaagtttgct
     3421 gattttgaga tttggcacgg aagtgagagt gaatatgatg attttagaac cgttagtcag
     3481 atgtcagagc aagcgttcac cctaactaac gaggaagatt tttggtgtat cggcaaaaat
     3541 gtggaaaagg acgatttgga taacattgat gtagtagaat ttatctacca aaccattcgg
     3601 gcattattgc cactttatga gaaatgtcat caataaacac taaaaagaga ggcgaacatc
     3661 atttgttcgc ccctttcaaa atttaagcta tcacttcttt tttattttca atgcgtaaac
     3721 ggtagcgttt atccaacata cagagtaaac caccgagagc cataaacagc ccgccaatcc
     3781 aaatccagcg aatgaacggt ttataataca aacgcactgc ccaagagtta tcttctaatt
     3841 tttcgccaag tgcagcgtat aaatctcggg taaagcccca gtcaatcgcc gcttcggtca
     3901 ttcccattcg gctcaccgta tagaaacgtt tttcagcatt aagtgtggct tcatatttac
     3961 cgtttcgagt aatttcaagc tcggcagtgc cgccttgata gtttgcccca tcggtgattt
     4021 taataccttt aaattcaaat tggtaatcta aaatctcagc tttatcaccc acattcattc
     4081 tgacatctcg ttcaatgctg aaattttggc taaatgcaat gccgaacact gtcatcgcaa
     4141 cccctaagtg agccagcacc attccccaat gtgaacggga cagtttgaaa atgccactta
     4201 agaacgagtg gcgatgggtt gcacgttggt gtagctcata caggctaagt aacacaataa
     4261 agacagacat catcgtgcct aacaccacac tcacggtaat ggtgttacgg aataagtatg
     4321 gcaatccgaa tccaagcaat gccattaaaa tagttgcaat aattaccggt ttacgaatag
     4381 cagaaacttg gtctctacgc catttcacta atggtccaat ccctaataca aatgagaaag
     4441 gaaccattaa aattaagaac atttggtcaa aaaatggtgc cccgattgaa atcgtcccta
     4501 agccgatttg tttgtgaatt aatggcagta aggtccctaa aaacaccacc gataaaaatg
     4561 ccattaataa aatattatta atgagtagca tcgtttctct tgaatagcgt tcatagttat
     4621 ctaagctttt aatttccgag cctttgaatg catacagcag taaagaacca cctactacta
     4681 acaccaagta agctaagatg tataaaccac gagttggatc tgaagcaaag gcgtgaactg
     4741 acaccaaaat gcctgaacgc actaagaaag tccccactaa acagagcgag aaagcaagaa
     4801 tcgctaataa gaccgtccac gctttaaatg tgcctcgttt ttcagtcact gctaatgaat
     4861 gaatcagtgc tgttcctgcg agccacggca ttaatgatgc attctcgaca ggatcccaaa
     4921 accaccagcc accccaacca agttcgtagt atgcccacca agaaccaagc acgataccga
     4981 gcgttaaaaa tacccacgct gccattgtcc acggacgaga ccatcttgcc cacgcagtat
     5041 ctaaacgccc tgtcatcagt gaggcaatcg caaaagcaaa ggcaacggag aaacccacat
     5101 agcccatata aagcaacggt gggtggaaga tcaagcccac atcttgcagc attgggttta
     5161 attctttgcc atcaatcgga aaatttggga aagttctatc aaacgggttg gaggtaaaaa
     5221 tcacaaacat caaaaagccg atgccaatga cccccattag tcctaacact cgagcaacgg
     5281 catcttccgg cattttacgg gtaaaggttg ctaccccgac agaccaaagc gacaacaacc
     5341 aaacccaaag caataatgag ccttcgtgag cgccccacac cgcagataaa cgatattgca
     5401 acggtaaacg gctattggag ttgttcacca catattgcac ggtaaaatca ttcaccgcaa
     5461 acagataaaa cagcgaacca aaagagacag tcaatgccaa aaacattgcc caagtcatcg
     5521 ggcgggcaag agcaattaag gtgctatttt gcttttctgc tccccaaagc ggtaaaactg
     5581 ccaacaagat agaaatcgct agggctaatg ccaacgtata attgcctaat tctgcaatca
     5641 tttgctttcc ccttgcacat tgttggcttt ttctgcttcc gcctcggctt tcattttgcg
     5701 gtcggtttcc gattcacctt tcagatcttg ttcagacaca cctgattttt tatgctgttc
     5761 ttttaattta tcgcctaatt ccggtggcat atagttttca tcgtgttttg ccagaacttc
     5821 cgtagctttt aaacgggtta gttcaatcaa cacgccttgt gccacaatgc cttgcccttc
     5881 acggaataaa tccggcaaaa ttccttcgta ttccacggta atcgaaggcc cgatatcatt
     5941 gagatcaaat actactttta aacttttatc atcacgcttt accgtatctt ccactaccat
     6001 tccgcccaca cggatacgct gaccaacttc aggcttttgt ttcggatcat catttttacc
     6061 gtttacaatt tcagacggcg tgtaaaacag atcgatattt tggcttaacg cataaagtgt
     6121 caagcctgct gcgacagaaa gcccaagtaa tatcgaaacc accactttta atcttgattt
     6181 gcgtcttgga ttcatcgtaa tcacccttca aaataaatgc aaaattgcat aaaaactcaa
     6241 aaataaatta gtgtaagaat agcaaaaaac cgcaaaacat tcccgatatt tcaaggaagt
     6301 tttgcgattt ctcaaatcaa atacctataa agaagtatga ggctatcgtt gcaaaaaata
     6361 aagttatttc gaccgcttgt ttattgtgat gtttgcttta atttttcttc acgcacaatc
     6421 tctttttttg cttgagccaa aatcgctttc tgttcacggc gactaagcca aatcagccca
     6481 atcattgcaa tgaaactgac accataagag agccatacat aaaagccgta gccacccatt
     6541 gcaaaaaaat cgctgattga ttcaaattga aatgtcattg ttctctccta ctctaaactg
     6601 attttgccag ttgttttacc caagggcgat tacgttcgtc ctttaataag gcaatgcgat
     6661 aacgccaaat gctaaaccaa gcggtaaaaa ttaagctacc gaaaatcgcc aatagcaacg
     6721 gaattaacat ttccaccgcc atagacggtt tatcgaattt agtgatggtc gccccttgat
     6781 gcaaggtgtt ccaccattcc accgaaaagt gaataatcgg caagttaatc acgccaacaa
     6841 ctgacagcac agccgccgct ttggctccgg tttgtctgtc ttggaatgcg gaataaagag
     6901 ccatcacgcc gatataaagg aaaaataaca ctaaagcgga ggttaaacga gcgtcccaca
     6961 cccaccaagt cccccacatt ggtttgcccc aaattgcacc ggttgcaagt gaaataaaag
     7021 cgaatactaa gccgataggt gccattgaaa tcatcgccaa gttagcttgg cgaatttgcc
     7081 aaaccaatgc caccaagcct gccaccgcca ttgagccata tacgcccatc gaccaaattg
     7141 ctgccggcac gtgaataaag ataatgcgat agctatcgcc ctgttggtaa tctttcgggg
     7201 caaaagccaa gccccaaaca aaagcagccg ctaacataat caaacttagc caacctaaaa
     7261 acggttggat tttccctagc aaatggtact gcgtttccga ttttgcataa gggtgtagcc
     7321 atttccacat atatttctcc aaagtaaata attcccctca cccgtttaca ggagagggac
     7381 gatttgagct tcgttcagaa gcgaaaatca gggagagggg aaaaagttgc aaattttcat
     7441 aataattcga ccgcttcccc tctctctgaa aaacacctct gaacgaggtg tttttcggtc
     7501 tctctccctc aagagggaga gaggaagttt tagttcaaac taattcttaa tgcaccggca
     7561 atcgcaaacg gcgaaaaagt taaagtaacc gctaaaattg ctccgataat cgccagttgt
     7621 ccgctataac ccatatttaa ggtggcggct tctaataccg cagcggcaaa aatcagtacc
     7681 ggcaagaata acgggaggat taataaactc agcaacgtac cgcccttgcg taagcccaca
     7741 gtgagtgcca cgccaatcgc tcctaagcag cttaaaatcg gggtgccgag taataatgtc
     7801 agcactaacg cccaccaaac gtgggtttca agtgaaagca aaatggcagc aatcggtgaa
     7861 agtaaaatca acggtaagcc ggtcagcagc cagtgagcaa tcactttcgc taacgcaacc
     7921 tgcggcaagg caagcggcag taacattaat tgctcgagcg aaccgtctaa ataatcatca
     7981 cggaataacc gctcaaagga aagcaatgca gaaagcaaag cagctaccca cgcaatgccg
     8041 ccggcaattt tgcctaataa ttcggggtta ggtcccatta aaatcgggaa tacggtaatc
     8101 accattaaaa aaaaccacaa cggatttaaa atctccgagg gtttacgaaa ggcaatggtg
     8161 agttcccgct gaataatagt caataaaatc atactttaaa ttgatctaac gaaataatac
     8221 gcactttgtc gctttcagca gcctggtggc tggtaaaaat cactaatctg ccttgctcgc
     8281 aatgctgttc aatatgagca atcagatccg ccacaccctt tttatcaata gcggtaaagg
     8341 gttcatctaa aatccaaagt ttggcgttag tgagccacaa ttttgccaac gccacacggc
     8401 gttgctgacc ggctgacaaa tgcgaacaag gcaaatcttc acgcccgatg agcgacactt
     8461 tcgtcaatgc gtgccaaagg gcatcatcat cttgcggtaa ctgttggatt ttctggtaga
     8521 aacgcaaatt ttcccacggt gttaattccg gttttacccc cgaataatgc cctaaataaa
     8581 atagttcaga ataatagtga tcacgctgtt tttgaattgc aatatcgttc cacagcacct
     8641 caccttccgc aggtaaagaa agcccggcta aaatacgtaa caggctggtt tttccaatgc
     8701 cgttatgccc ttcaatctgc acccattcac cactttgcac ggtaaaactg cacccttcaa
     8761 acaaacgggt ttcgccccgt tcgcaggcaa tctgattaag tttaagctga tattgggccg
     8821 tttggttcat tatttttcat ctggttgaac attttagccg aagtgtatca taagcctttg
     8881 atttaacgta gcaaataaat tacttcttta gaggtagcaa gcggtcaaat tttcaaaaag
     8941 ttttgcaaat tttgataaga atcaaaccgc ttgttacaga gagaggattt ccttcacaaa
     9001 aggaatggtt aatttacgct gggcttggag agacgcttta tccaacttat ccaatatttt
     9061 agagagttgc tctaaatccg ttcccgcttt ttttaacaga aaatgagcga cctcatcgga
     9121 caattccacc cctttttggt gagcatttaa ctgtaaaatt tggcattttt gttcatcggt
     9181 taaatcgttt agctggtaca cttcgcccca agttaagcgg gaacgtaaat ccggcaattg
     9241 gatattgagt tggtgtggtg ggcaatcggc actgattaac agcaaggttt tttgcccttc
     9301 gttaaacaag ccttgttgtt ctcgaatttg gttaaacagg ttaaaaatag caagttccca
     9361 ttcagcatct cctgcaatcg cttgaatatc atctaggcaa atcacattca gcagttcggc
     9421 attatctaac accaacggcg agaaataatt ggctttagtg agcggaatat aactagaagt
     9481 ttggttattt aaaagaaagt ggttacttac tgctttcaac aaatgacttt taccgcagct
     9541 cttaccgccc caaatgtaaa aaaacggctg ttgaacatcc gtaaaatttc gtttgaggga
     9601 ttcaagcaac acttcgctgt tttggctata aaaattgtcg aaagtgtctt catcaatttg
     9661 atgaatgggt agaggtaact gcaaggcttt ccttaaatct ctaagtttga tatttttcta
     9721 agaatagcgg aaaagcaggg gtttgagaag taaaaatagt gagaaaatat ccactcattt
     9781 ttctaatgaa gagatgacaa gcggttgaat ttccacataa ttttgtcaat ctgaccgaaa
     9841 acccaccgtt ttgtgttaaa aaaagattgc agttagctca aaaaattgta tattcaaagc
     9901 tttctactta aaaagtagag taaaacaact attacaaaca caacggagtg aatatgaaaa
     9961 acatgactaa attatcgcta ataacattag cgctagccgc ttcaagtagc gtctttgctg
    10021 caccgaaaac ctttgtttac tgcttagagg cttcgccttc agcattaagc ccagtgctat
    10081 caacagacgg tgcaagcttt gatgtgaacg ccctcccgat ttataacaaa ttaaccgact
    10141 ttgaagacgg tacaactaat gttattccct ctcttgccga aaaatgggac atttcggaag
    10201 acggcaaaac ttacaccttc cacctgcgta aaggcgtaaa attccacgac aataaagaat
    10261 ttaaaccaag ccgtgagtta aatgcagacg atgtagtgtt ctccattaac cgtcagtttg
    10321 atccaaacca tccattccac aaagtttccg gcggtaacta cgaatacttt atcggtatgg
    10381 atatgcaaaa tattatcgat aaagtggaaa aagtggacga tcacaccgtt aaaatcagct
    10441 taaaagtgcc aaatgcgccg ttcttagcta acttagcgat ggatttcaac gccgtttact
    10501 cagcggaata tgcagagcaa tccttaaagg ccggcaagcc tgagcgtatt gataccaacc
    10561 caatcggcag cggcccgttc cagtttgtgg attaccaaaa agatgccacc gttcgttaca
    10621 aagcctttga aaactactgg caaggcaaag cgaaaattga ccgcttagtg tttgcaatta
    10681 ctccggatgc atcggtacgt ttagccaagt tacaaaaagg cgagtgccac gcaatgcctt
    10741 accctaaccc ggctgatatc gaaaatctga aaaaagatcc aaatattgaa ttaatgagcc
    10801 aatctggaat gaatatcggc tatttggcat taaattcatc gattaaacca ttagataacc
    10861 aaaaggtacg tcaagcatta aatcacgctg taaacaaaca agcgattgta gatgcggtat
    10921 atcagggagc cggtcaagta gcgaaaaacc caattccacc aacaatgtgg agctataatg
    10981 aagcggttca agactatgac tacaaccctg aaaaagccaa agcattgtta aaagaagccg
    11041 gttttgaaaa tggttttgat actgaacttt gggcaatgcc ggtatcacgt ccatataacc
    11101 cgaatgctcg ccgtatggct gaacttatcc aagaagactg gaaaaaagtg ggcgtgaatg
    11161 cgaaaatcgt cagctatgaa tggggcgaat atctcaaacg cttacgccaa ggtgaagcgg
    11221 caacctcaat gattggctgg acaggcgata acggtgaccc agataacttc ctcaacacct
    11281 tattaagctg ctcagcggtg gaagccggtt caaactacgc caaattctgc cacaaaccgt
    11341 ttgaagaagt cgtgcttggt gctgcccaag aaagcgacaa agcaaaacgt accgagcttt
    11401 ataaacaagc tcaagtgatc ttcaaagagc aagctccttg gattacgatt gctcactcca
    11461 ccgtttactt cccggtgcgt aaagaagtaa aaggttatgt gatgagtccg ttcagcttac
    11521 ataacttcta caaagtagat ttagcggata aataatccgc caacaagcgg tagaatttgc
    11581 agattttttt acaaatttga ccgcttgtcc tcccctcttt gaactacgag gggcaggggg
    11641 gagatttggc agaagcgatt gccgaagtga gaggattgta aaagaggaaa taaattttct
    11701 gtcttattga ttattttctt cgttaattac tactgtcaaa tctcccctac cccctctttg
    11761 ctaaagaggg gaatcagatc gagagatcag acaactttga tgaaaatcca acctaagaga
    11821 ttttaaatgt tttcatttat tttaaaacgt gtgctaatgg tgatccctac ctttatagcg
    11881 attacgttaa ttacttttgc ccttgtgcat tttatccccg gtgatccaat tgagatccga
    11941 atgggggaac gtggattatc gcctgaggtt catcaacaaa tgatggaaca actcgggctg
    12001 aaccgaccgt tgtttgaaca atacatcagc tacatcaaag gtgtattgca cggtgatctt
    12061 ggtaactcat ttcgtaacaa cgaaccggtg ctgaaggaat tttttaccct attcccagcc
    12121 acggttgagt tggcattttt tgccctgctc tggtcgttga ttttcggcat tattctcggt
    12181 gtgattgctg cggtgaaaaa agaaagttgg atttcacaca ctgtaaccac gctttcactg
    12241 accggctact caatgccaat tttctggtgg ggcttgattt taattcttta tttctccacc
    12301 ccgctcggct taccggcttc aggtcgttta ccatctgaat actggattga agccgaaacc
    12361 ggttttatgc tgattgatac ttggaattcg gacgaaccag gggcattttt agcggcaatt
    12421 aaatctctga ttttgccgtc tatcgtactg gggacaatcc ctcttgcagt tgtgacccga
    12481 atgactcgct cttcaatgtt ggaagtgttg ggtgaagact atatccgcac cgccaaagcc
    12541 aaaggcttaa acactacccg aattgtgatt gttcacgcat taagaaatgc cttgattccg
    12601 gtggtaacgg tcgttggctt aattgtcggg caactgcttt caggagccgt tttaacggaa
    12661 aacatcttct cgtggccggg tatcggcaaa tggattattg atgcaatcaa cagccgtgat
    12721 taccctgtac tacaaggctc agtgctgatt attgctacta ttattatttt agtgaacctt
    12781 acggtagatc tactctatgg cattgtcaat ccgaggattc gacatagtta gaaaaattcc
    12841 ttcgattttt cccctcttta gcaacgaggg agttacacaa gcggttcaaa ttccgcaatt
    12901 ttttacaaga ttatttttag ttggagttaa tgatgtcagg aataacggcg gtaaatccac
    12961 ccgcacccaa aacaccgtta caggagtttt ggtactattt ttgtcaaaat cggggggcat
    13021 taataggact tacctttatt gcaatcgtct ttgttgcgtg cgtattcgca ccttgggttg
    13081 ccccttttga tccaatcgaa caaaatcgta gtgcgttatt gctccctcct gcttggcttg
    13141 aaggggggaa ttccgctcat ctgctgggta ctgacgatat tgggcgtgat attctttccc
    13201 gtattattta tggttcaaga ttgtccgtct ttatcggctt agtcattgtg ctactttctt
    13261 gcgtattcgg cgtgatttta ggtctacttg ctggctacta tggtggcaca ctcgacatca
    13321 tcattatgcg ttttgttgat attatgctgg cgattccaag cctgctttta actatcggcg
    13381 tggtcactat tttaggccct tcattgataa atgccgctat tgcgattgcg attgtctcca
    13441 ttccaagtta tgttcgcttg acccgtgctt ctgtattaag cgagaaaaac cgtgattatg
    13501 ttactgcttc tcgtgttgct ggtgcgagcg tgcttcgctt aatgtttgtg gtgattttac
    13561 ctaactgttt agctccgctt atcgttcaga tgacgatggg gatctcaaac gctattttag
    13621 aacttgccgc cctcggcttc ttaggcattg gtgcacagcc gccaacaccg gagcttggca
    13681 caatgcttgc cgaatctcgt ggctttatgc aatcggcaaa ctggctggtg accattccgg
    13741 gtctagcgat tttatcgtta gtattagctt tcaacttaat gggtgacggc ttgcgtgacg
    13801 ccctcgatcc aaaactgaaa caataggaga tctaaaatgg cattattaac ggttgatcaa
    13861 ctttccgtcc attttggtga aactaaaaca ccatttaaag cggtggatcg tatcagttac
    13921 gaagtcaatg aaggcgaagt attaggcatt gtgggcgaat ccggttcagg aaaatcggta
    13981 agttctctcg ctattatggg cttaatcgat ttccccggca gggttatggc tgaaaaattg
    14041 tattttaacg gcaatgattt attggcatta aaaccaaaag aaaaacaaca gattgtaggg
    14101 gctgatgtct caatgatctt ccaagatgca atgaccagct taaatccgag ctatactgtg
    14161 ggctatcaga ttatggaagc cctaaaagtg catcaaggtg gctcaaaagc cagccgtcgt
    14221 gagcgagcga ttgagttgct gacgatggtc ggtattcccg atcctgaatc ccgtttagaa
    14281 gtttatccgc accagctttc gggtggtatg agccagaggg tgatgattgc gatagcgatt
    14341 gcctgtcgcc cgaaattatt gattgccgat gagccgacta ctgcgttaga tgtgacgatt
    14401 caagctcaaa tcattgattt attacttgaa ttacaacgca aagaaaatat ggcgttgatt
    14461 ttaatcacgc acgatttagc gttagttgcc gaatctgccc atcgcattat cgtgatgtat
    14521 gcagggcaag tcgtggaaga gggcaaagcc gagcagatat tcaaagcacc actgcaccct
    14581 tacacccaag cattgctcaa agccttaccg gaatttgccg aagggaaatc ccgcctgcaa
    14641 tcactaccgg gcgtagtgcc gggcaaatat gaccgcccac aaggttgctt actcaaccct
    14701 cgctgcccgt atgcaaccga aaaatgccga accattgaac cggcaatgca gatgatcaac
    14761 ggcagacagg tgaaatgcca cacgccactg aatgcacagg gtgagccgat aaggatagct
    14821 gtataacgtc ataaagagta gggacgcaat gcttcgcgtc cgttacaagc ggtgattttt
    14881 aaccgatttt tgcaaaataa cgatagtgga cgcaaagcat tgcgtcccta cattttccga
    14941 gaaggaaaac agaatgaaag aaaataattc tctcccacca ctcctcaaag cagtggactt
    15001 aaaaaaatac tacccagtta aaaagggaat gttcgctaaa ccgaaaacgg taaaagcgct
    15061 ggatggtgtc tcttttaccc ttgaacgtgg caaaaccctt gcgattgttg gcgaatccgg
    15121 ctgtggaaaa tcaactttgg ggcgaatgct cactatgatt gaaagcccaa ccgaaggtga
    15181 actgttctac aacgggcaga atttcttagt caatgacaaa gaaacggctc aactacgccg
    15241 taaaaaaatc caaattgtgt ttcagaaccc gtatagctcg ctcaatccac gcaaaaaagt
    15301 aggtagcatt ttagaagaac cattattgat taacaccaac ttatccgcaa aagaacgcaa
    15361 agaaaaagtg ttggaaatga tggcgaaagt cggcttaaaa ccggaatttt acgaccgtta
    15421 tccgcatatg ttttcgggcg gacaacgcca gcgaattgcg attgctcggg gcttaatgtt
    15481 gcagcctgat attgtggttg ccgatgaacc cgtttccgca ttagatgttt cggttcgggc
    15541 gcaagtgttg aatttgatga tggatttgca agaagaaatg ggcttgtctt acgttttcat
    15601 ctcgcacgat ttatccgtag tggaacatat tgccgctgaa gtgatggtga tgtatttagg
    15661 gcgttgcgta gaacaagggg ataccaaaac tatcttcagc aatccacgtc acccttatac
    15721 acaagcgtta ctctccgcaa cccctcgttt gaacccggag caacgccgtg agcggattaa
    15781 actgactggc gaactaccaa gcccgcttaa tccaccaaaa ggctgtgcct ttaacgcccg
    15841 ttgcagattt gcaaccgatc gttgctatgt ggagcgacca gagctgaaaa cctatcaaga
    15901 cggtactcgc atggcttgtt ttgtggtgga ggaaaataat taggcaacgc tcgctattta
    15961 aaaggttaaa gtttttcaac tttaaccttt atttttcata cttaatgctc ctcatctttt
    16021 gtagcaaaat ctttcattaa tttgctgata tgctcacaat ggtgtttaaa ttgacgacaa
    16081 tacgggcaaa taatcaaatg cgttttaacg ccaatcttct cttttaaagt gagttttcgt
    16141 ttctttgagt cagaaattaa ccttgttact tgtcggcaat tcatctattt ctctccgtta
    16201 atttccttga aagacattgt tgtaactgca gccttgctcg atagattaat gtatataaat
    16261 tatctgatgt aatatttact actcgacaaa tttcatctga ggttgcctct aaatattccc
    16321 tcatcataaa taccctagct tgttttgcag gtaaattgct taagcaaact tcaaataata
    16381 tccaaaattc tttggaataa acagcttctt cctaccagct agaaggataa tattcttctt
    16441 tccaatgccc ataattatca aaaaagtgat tctcttcgtc atcatcagct agatcacttt
    16501 ctagtactat acgatctttt ttacgtaaat aatcaataat tttatttttt agaatagcaa
    16561 atatccaggt tttcaatgct gctttacgct ggaaattatc aatatttttt attgcattaa
    16621 cgaatgcttc ttgaaccata tcttcagcaa gagcaatatc ttgtaattgt aactgagcaa
    16681 atttcaacat ttgctgacga atatcggcaa gctgttccga tgaaatatct aa
//
LOCUS       DANJWC010000042        16234 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.042, whole genome shotgun sequence.
ACCESSION   DANJWC010000042 DANJWC010000000
VERSION     DANJWC010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16234
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.042"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..188)
                     /locus_tag="PWX02_000980"
     CDS             complement(<1..188)
                     /locus_tag="PWX02_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729008.1"
                     /translation="MSIKKLLSTTIDQLENIIQVSGESFEKITSDLAEKAKELNSEYN
                     IKCEKLGYAITLFEKNSE"
     gene            complement(566..1600)
                     /locus_tag="PWX02_000981"
     CDS             complement(566..1600)
                     /locus_tag="PWX02_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDL1729009.1"
                     /translation="MKTVIADNSAEFVNEISQLIQSSKQRMTVAVNAELTLLYWHIGK
                     RINDYILQGERAEYGQEVVKNLAQSLTEQFGKGWSKRHLNYTMQFATTFPNLEIVHAL
                     RAQLSWTHFKQIIHIDDPTKRGFYATMAAEERWSTRTLEDRIGSLLFERTAISKKPDE
                     TIIAELSLLREKGEYNQSMVLKDPYILDFLDLNDRYMERDLEDAILREIEQFLLELGA
                     GFTFVARQKRIQIDNDDFYIDLLFYNRKLKRLIAIELKTESFKHSHKSQMELYLAWLA
                     KYEQEADENPPLGMILCTGKKQEQVELLDLKNSDIHIAEYMTVLPSKVVLEQRLHLAV
                     ERAKERFLLK"
     gene            complement(1643..1903)
                     /locus_tag="PWX02_000982"
     CDS             complement(1643..1903)
                     /locus_tag="PWX02_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729010.1"
                     /translation="MKPILYFADWCPDTAPFVAELDRLGIEYEPCDITKGGSTLKPFL
                     KLRDTHSAFDHAKANGYIGIPALLIEGEKVVLDLAELEGIFG"
     gene            complement(1983..2891)
                     /gene="glyQ"
                     /locus_tag="PWX02_000983"
     CDS             complement(1983..2891)
                     /gene="glyQ"
                     /locus_tag="PWX02_000983"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017806840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDL1729011.1"
                     /translation="MSTKFNVKTFQGMILALQDYWASKGCTIVQPFDLEVGAGTSHPM
                     TCLRALGPEPMAFAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLDS
                     LKMLGFDPTQNDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPV
                     TGEITYGLERLAMYIQGVDSVYDLVWSDGELGKTTYGDVFHQNEVEQSTYNFEYADVD
                     FLFQCFEQYEKEAQYLLGLEKPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILR
                     IRALTKGVAEAYYASREALGFPGCKK"
     gene            3086..3697
                     /locus_tag="PWX02_000984"
     CDS             3086..3697
                     /locus_tag="PWX02_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentahemic C cytochrome"
                     /protein_id="HDL1729012.1"
                     /translation="MKSLSKIAFCFLAFIMPTFCFAGSNSYTTETIPLFADENLTQKI
                     GQLEAGAEIKLVQTTAKAAEIELNLWRKSKGFGRIWYHSFSKNITDAVFEKSFISNNP
                     LLEIVESKEDPNTGLVWQKVKMNVWVENAEFTQDLTNFWANTKNTYKTECSVCHKQRD
                     PKMHDANEWIAVFNGMVGFTDMDEATQKKVLRYLQMNAASSSQ"
     gene            complement(3781..4398)
                     /locus_tag="PWX02_000985"
     CDS             complement(3781..4398)
                     /locus_tag="PWX02_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL1729013.1"
                     /translation="MILVCGANGLLGRAICDKLPREKTIAVLRKDAMDTFFSERNIAV
                     EIADLLDLESCNALLQKTQPEILITVVGGKNEQGIRSDKTANINLIKAVEKYSPTTKV
                     VLVTSVGCAEQWDLISPLAQQALGEALKAKTEAEAYLQQTALNWLIIRPGGLNNDEVN
                     DEYQIVRDLPNERKMYVSRKSVATGIVRLLDSTENHQIFSIINRG"
     gene            complement(4401..6218)
                     /locus_tag="PWX02_000986"
     CDS             complement(4401..6218)
                     /locus_tag="PWX02_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HDL1729014.1"
                     /translation="MIIKDTHWTSQQTSPNAFPERQALMPIWGGKNIERNQWQSLWLE
                     QAPNALLQDGLAYFHIPFCASHCIFCGFYRNAWKENYSQIYTDRVISELRYESSLWRG
                     QGKIHAVYFGGGTPTALHTEDLVRLIRACYRYLPLAEDCEFTIEGRISHFDIDKAKAC
                     IEAGVNRISIGIQTFNSEIRKRLGRKHSGEEAQTYLKALSELNAVIVADLIFGLPNQT
                     DDIWQNDIEIATSLPISGLDIYAFNNYPFLPINRLIEHGKLPAPAGFDTQSLHYAYAV
                     EKLKAQGWQQVSNNHFAFPNRGERNRYNTLVKSNMPCLAFGSGAGGNFGGLSYQVQSD
                     LKTYLDSPLDQKSLSFLSKHGEHKALLGVIQHSMELGYLDTTPFEQNAKAEKLLQQWE
                     ALDLLKREKNQVYLNTSGRYWSPTLIRKLMLTLPQSSKDNNMITPLTKELENKLRTNL
                     KENPGQILEMLAGMNQCSLESVINCLPKEMVMQTGGERFVEIMQAIAGWNEAVTFIIH
                     TPDAIAEVTAKLPQGKIGRGFYNFEHGVDGGIHGHLRYENCAAIYLLDRPFMGKRTVA
                     LTFINQSGEAMFKIFVGRDEQGQLRENQIQAMRKLIGGC"
     gene            complement(6375..7064)
                     /locus_tag="PWX02_000987"
     CDS             complement(6375..7064)
                     /locus_tag="PWX02_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005577085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HDL1729015.1"
                     /translation="MFMYLSHPLDPKDLAWPGEPVVEVTRCTDVGEETPFSSFITKLP
                     NHCGTHMDAPRHFVKDGLSINELPIEYFCHKEVALIEIPKGEAEGITKEDLLPYVDIL
                     SKVSFAFLRTGFEKYRTENPAIYQNECPYISTGAGQYLSDTFPNLKGVGIDFLALGSP
                     SSRVPDAENPKNCHRAILGYYTGRFTTVIEDMHLAELPKNANIKQFFNAPWRIKGLDS
                     SQVTCIVELQQ"
     gene            complement(7076..8416)
                     /locus_tag="PWX02_000988"
     CDS             complement(7076..8416)
                     /locus_tag="PWX02_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HDL1729016.1"
                     /translation="MLKKSLLCMLLISSALYAEDRVQENQLTNITLLAEITAQGQKIN
                     GVALEYPDNVLSGSDLRQLYQVKTALDSAEKENRSVLKAYVNNEPKVTALPQAGKFVI
                     IELDTADKNAATYSVKDANKAPMKFKSKDENGNIVETEKVQAVKIPEFFANRLIYSVE
                     QKGLLKLTSGTTLGKAEWTQTAEPDKIKTPYLDDFVAKQVSLDKPENKLNYRFYSPLQ
                     KNGEKYPLVIFLHGSGQVGSDNLAHLLSSKGAIATLPYAPSFVLAPQYDQVFDPFDNA
                     AKGQKGGIHWQTENRINLVLKMIDETLKANPQIDTTRIYINGLSRGAEGGLNLLLARP
                     NFFAGALLMSGREAYTNEWVDGNATKTNLAAIKHIPIWFFHSKEDKVSPVKGSRINYR
                     ILKDELNAPNVKYTEFTFEQAGDNGIVNNNPHNTWDAVYHSPEVIGWLLQQKRH"
     gene            complement(8478..9836)
                     /locus_tag="PWX02_000989"
     CDS             complement(8478..9836)
                     /locus_tag="PWX02_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntT protein"
                     /protein_id="HDL1729017.1"
                     /translation="MSNEMLILIGLASVVALLVIMIKGKVHPFLALGLVSIAVALTAG
                     IPMNKVIPTLISGMGSTLGSVALIVGLGAMLGKIIEKSHGADVLANWLLQRFGEKNAP
                     FALGMTGFIFGIPVFVDVGFIVLIPIIFSVARRLGGNILVYSLPIGLSMLLVHVLLPP
                     HPGIVAGAQNLNADIGFVLGIGLVAALPAYLIGQKFIPFFTKNYFVKVVTSTDLLQHQ
                     KDLIKEKGIELPSFGKVLAMISFPLILIMLGTVTATTLPKEHIVREVFSMVGSSPFAL
                     FVAVCLSAYVLGFRYGWTKHKLEEMLDSALAPIAGIILITGAGGMFGKVLDASGVGKA
                     LADVLANTGLPLLVLAFILSALLRAAQGSATVATITTTTILAPAAAAAGYTELQLALL
                     TCAIGAGSMTLSHVNDSLFWVWTKFFNVPVSVGLRTWTFLTTIFGVIAFAVVGIIWML
                     FV"
     gene            complement(9889..10665)
                     /locus_tag="PWX02_000990"
     CDS             complement(9889..10665)
                     /locus_tag="PWX02_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005547735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase family protein"
                     /protein_id="HDL1729018.1"
                     /translation="MPKFAANLTMMFNEVPFLDRFEAAANAGFKYVEYLWPYDYPAAE
                     LKAKLEQYGLKQVLFNTAAGDVAQGEWGVSAIPGREADSHRDIDLALEYALALGCKNV
                     HVMAAVVPEGEDREAYKQTFIENVQYAADKFKPHGINVLLEALSPEVKPNYLLKSQFD
                     TLEIVEAVNRDNVFVQLDYFHAQNVDGNLSRLTDKLKGKFAHIQIASVPDRHEPDEGE
                     INYQYIFNKLDEIGYEGYVGCEYKPRAETTSGLGWFEQYK"
     gene            complement(10667..11299)
                     /locus_tag="PWX02_000991"
     CDS             complement(10667..11299)
                     /locus_tag="PWX02_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014668373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDL1729019.1"
                     /translation="MTEQEQKIEMVNLARSLFERGYTVGGAGNLSVRLDENRILVTPT
                     GSSLGRLEADRLSVLDMQGNVLSGDKPSKESVFHLAMYQKNLACQAIVHLHSTYLTAL
                     SCLENLDTDNAMRAFTPYYVMRVGKLPVIPYYRPGDINIARELGERALDGKAFLLANH
                     GVVVTGSDLRDAVDNTEELEETAKLHFILQGQKVRYLTDDEVNDLKNRGK"
     gene            complement(11296..12537)
                     /locus_tag="PWX02_000992"
     CDS             complement(11296..12537)
                     /locus_tag="PWX02_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005754893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HDL1729020.1"
                     /translation="MLGIIADDFTGASDIASFLTENGLKTVQMNGVPKTTLNSQVDAI
                     VISLKSRSNPVEEAIQQSLQALDWLKQNGCSQFYFKYCSTFDSTEKGNIGPVTDALMA
                     ALDTDFTVVTPALPVNGRTIFNGYLFVGDVLLNESGMRNHPITPMKDANLVRLMDAQS
                     QGKTGLVAYADVIQGAERVKARFAELKQQGYKYAVVDAADNSQLAVLAEAVADFQLVT
                     GGSGLGAYMAAYRSGGKKGTNAFVPSKGKTVVLSGSCSVMTNTQVAAYQQKAPSYFLE
                     AEKAIQDPNYVETLYQWVIANLDGDFAPMVYATVPPEKLKEIQTQFGGDKASHAIENT
                     FAKLAEKLQAAGVTNFITAGGETSSIVVQKLGFSGFHIGKQIAPGVPWLQAVEKPIFL
                     ALKSGNFGKEDFFSFAQEMFV"
     gene            complement(12537..13442)
                     /locus_tag="PWX02_000993"
     CDS             complement(12537..13442)
                     /locus_tag="PWX02_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL1729021.1"
                     /translation="MSQKYSVAVIGLGAMGMGAAKSCIAAGLDTYGVDLNPNYLETLK
                     AAGAKKVGTSAVDFAAELDAVLLLVVNANQVNAVLFGENGLAQHLKQGTVVMVSSTIS
                     AQGAKDISQKLTDLGLVMLDAPVSGGAAKAAQGEMTVMASGSSEAFEKLKPVLDAVAG
                     KVYNIGEEIGLGATVKIIHQLLAGVHIAAGAEAMALAARAGIPLDLMYDVVTNAAGNS
                     WMFENRMKHVVDGDYTPLSMVDIFVKDLGLVNDTAKSLRFPLHLASTAYSMFTEASNA
                     GYGKEDDSAVIKIFSGIELPKKGDN"
     gene            13616..14374
                     /locus_tag="PWX02_000994"
     CDS             13616..14374
                     /locus_tag="PWX02_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL1729022.1"
                     /translation="MIPLERQREILRLINNNGVASINFLAEQLKVSHMTIRRDIQKLE
                     EQGKVSLVSGGVQSLERLIMELPHDDKSMLFQEQKSAIGSLASQLIPSNTTIYLDAGT
                     TTLEIAHRIIDRNDLLVVTNDFVIAHYLSNSGKCDLIHIGGSVCKENLSTTGSLAAEF
                     LKNLSIDMAFISTSSWNLKGLTTPNENKIPVKKAILEASKQNILVTDSSKYGKVATFF
                     VCPLTVFDQIICDKGLFENVRDALKGLSIKLDLG"
     gene            complement(14514..15905)
                     /gene="ffh"
                     /locus_tag="PWX02_000995"
     CDS             complement(14514..15905)
                     /gene="ffh"
                     /locus_tag="PWX02_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDL1729023.1"
                     /translation="MFENLSDRLSKTLRNITGKGRLTEDNIKDTLREVRMALLEADVA
                     LPVVRDFINKVKERALGVEVNKSLTPGQEFLKIVQSELETAMGEANEELNLAAQPPAV
                     ILMAGLQGAGKTTSVGKLAKFLKERHKKKVLVVSADVYRPAAIKQLQTLAETLKVDFF
                     PTETSQKPVAIAEAALKHAKLNFFDVLIVDTAGRLHVDGEMMEEIQQIHAVLNPIETL
                     FTVDAMTGQDAANTAKAFNEALPLTGVILTKVDGDARGGAALSIRQITGKPIKFLGVG
                     EKTDALEPFHPDRVASRILGMGDVMSLIEDIQRSVDQEKAEKIAAKFKKGDEFTLEDF
                     REQLIEMKKMGGMMSMLDKLPGAKNLPDHVKSQVDDKMFMKMEAIINSMTLKERANPD
                     IIKGSRRRRIALGSGTQVQDVNKLLKQFDDMQRMMKKMRKGGMAKMMRGVKGMMGGGL
                     GGLGGLGSMFGKR"
BASE COUNT         4610 a         3601 c         3156 g         4867 t
ORIGIN      
        1 atttccgagt ttttttcaaa taatgttatg gcgtatccca atttttcaca tttaatatta
       61 tattctgaat ttaattcttt tgctttttct gctaagtcac ttgtaatttt ttcgaaactt
      121 tcgcccgata cttgtattat attctcaagt tgatctatag ttgtagataa taatttttta
      181 attgacatat atttccttta ttatatcctt ttctttgctt ttattgtact tatcaagaaa
      241 tgagagcaac tttatgatta acttgcaaaa aagtactaga ctaatcaatc tctcactacg
      301 ttttcacttc tgctaaatct cccctagccc ctctttgcta aaaaggggaa tttgatcttc
      361 gtaaatttat aattcatctt tcttcattaa gattgtaaat agtcagatga attaaaatta
      421 ctcactaaat gatcgccccc tctttagcca aagaggggga ggggggagat ttgaaaatct
      481 ctaaccaccc accaacggaa tatgcaacca aatcgtttcc atcacacttt cattttgcaa
      541 catttccgct gattttaacc gcttgctatt ttagtaaaaa ccgctctttc gccctttcca
      601 ccgccaaatg caaccgttgt tccaatacca ctttactcgg caacacggtc atatattccg
      661 caatatgaat atcggaattt ttcaaatcca acaattccac ttgctcctgc tttttaccgg
      721 tgcagagaat cattccaagc ggtgggtttt cgtctgcttc ttgctcgtat ttcgccagcc
      781 acgccaaata gagttccatt tgacttttgt gggaatgttt gaagctttcg gtttttagtt
      841 caatcgcaat caggcgtttg agtttacggt tataaaacag cagatcgata tagaaatcat
      901 cattatcaat ctgaatcctt ttttggcggg cgacaaaggt aaaacccgcc ccaagttcca
      961 gtaaaaactg ttcgatctct cgtagaatcg catcttcgag atcacgttcc atataacggt
     1021 cgttaaggtc gagaaaatcc aaaatatagg gatctttcaa caccattgac tgattatatt
     1081 cgcctttttc acgtagcaaa ctcaattcag cgataatggt ttcatcgggc tttttggaaa
     1141 ttgccgtgcg ttcaaacaat aatgagccaa tgcgatcttc taatgtcctt gtactccaac
     1201 gctcctctgc agccattgtt gcatagaacc ctcgctttgt tggatcatca atatgaataa
     1261 tctgcttaaa atgtgtccag cttaattgtg cacgcagtgc gtgcacaatt tccaaattag
     1321 ggaaagttgt agcaaattgc attgtataat ttaaatggcg tttactccaa cctttaccaa
     1381 attgctccgt cagactttga gcaagatttt tcaccacttc ttgcccatat tccgcacgtt
     1441 caccctgcaa aatatagtca ttgatccgtt ttccgatatg ccaataaagc aaggttaatt
     1501 ctgcattaac cgcaacggtc attcgttgct tactcgattg aataagttgt gaaatttcat
     1561 tcacaaattc agccgaatta tctgctatta ctgtcttcat tattggctcc tttgcaacat
     1621 ttccgccaaa ttcaaccgct tgttagccga aaataccctc taattcagcc aaatccagca
     1681 cgactttttc gccttcgatc agcaatgccg gaatgccaat atagccgttg gctttggcgt
     1741 ggtcaaaggc agaatgcgtg tctcgcagtt tcaaaaatgg tttaagtgtt gagcctcctt
     1801 tggtgatgtc gcacggctca tattcaatgc ccaagcggtc tagttctgcc acaaatgggg
     1861 cggtatccgg acaccaatcg gcgaagtata aaatcggttt catcgtaaat ccttgtaggg
     1921 acacgatgct tcgtgtccgt tttgtaattt attgacggac gccaagcatg gcgtccctac
     1981 gattattttt tacaccccgg gaaccccaac gcttcacggc tggcatagta cgcttcagca
     2041 acgcctttgg ttaaggcacg aatgcgtaga atgtagcgtt ggcgttcagt taccgagatc
     2101 gctttacgag catctaataa gttaaagctg tgagcggctt ttaaaatgcg ttcgtatgcc
     2161 ggcaatggca atggtttttc taggcctaac aggtattggg cttctttttc gtactgctcg
     2221 aagcattgga acaggaagtc cacatcagcg tattcaaagt tgtaggtgga ttgctccact
     2281 tcgttttggt ggaatacgtc gccgtaggtg gttttaccga gttcgccgtc agaccacacg
     2341 aggtcgtaca cgctgtccac gccttgaatg tacatcgcca aacgctctaa gccgtaggtg
     2401 atttcgcctg tcaccggttt acattccaag ccgccgactt gctggaagta ggtgaattgg
     2461 gtcacttcca ttccgttcag ccacacttcc caacctaagc cccacgcccc aagcgttggg
     2521 ttttcccagt tgtcttccac aaaacgaatg tcgttttggg ttgggtcgaa accgagcatt
     2581 ttcaggctgt cgaggtaaag ttcttgaatg ttatccggcg aaggtttaat caccacttgg
     2641 aactggtagt agtgctgtaa acggttcgga ttttcgccgt agcgaccgtc tgtcgggcgg
     2701 cgagatggtt gcacataggc aaacgccatt ggctcagggc caagcgcacg caggcaggtc
     2761 attgggtgcg aggtgcctgc ccccacttct aaatcgaacg gctgcacaat ggtgcaacct
     2821 ttgcttgccc agtagtcttg cagggctaaa atcattcctt gaaaggtttt tacgttgaat
     2881 tttgtactca ttttattttc tcttgaattt gaaaaatggt cgaaaaatac tggttattat
     2941 agcttgtaag cggtcatttt ttgggatttt tttgcaaaaa attaagaaat cgacataaaa
     3001 tcagttttat gatgtaaatc aacgcttttt tgctcaattt gtgtaacata taacctaatt
     3061 tctcatctaa tttgtggggt ttatgatgaa atcattatcg aaaatcgctt tctgtttctt
     3121 ggcattcatc atgccaacat tttgttttgc cggttcaaac agttacacca cggaaacaat
     3181 ccctttattt gccgatgaaa atttgacaca gaaaatcggg cagctagaag ccggagcaga
     3241 aattaaattg gtgcaaacaa ccgcaaaagc cgctgaaatt gagctgaatt tatggcgaaa
     3301 aagcaaaggt ttcgggcgga tttggtatca ttctttcagt aaaaatatta ccgatgcggt
     3361 atttgagaaa tcttttatca gcaacaaccc actgttagag attgtagaga gcaaggaaga
     3421 tccgaacacc ggcttggtat ggcaaaaagt gaaaatgaac gtgtgggtgg aaaatgccga
     3481 atttacccaa gatctcacta acttttgggc gaacaccaaa aatacctata aaacagaatg
     3541 cagcgtgtgc cataaacaac gtgatcctaa aatgcatgat gcaaacgaat ggattgcagt
     3601 atttaacgga atggtcggtt ttaccgatat ggacgaagct acccagaaaa aggtgcttcg
     3661 ctacttgcag atgaatgcgg catccagttc tcaataaaat agaaaaagat ctcacaagcg
     3721 gtctgtttta tagttgcaaa ataaccataa aatatgcccg cttgccttcg ttgaaatata
     3781 ctatccccta ttaataatag agaagatttg atgattttcc gtactatcca gtaaacgcac
     3841 tatgcctgtt gcgacagatt tacggcttac atacattttc cgttcattag gtaaatcacg
     3901 cacaatctga tactcatcat ttacttcatc attatttaac ccaccgggtc gaataatcag
     3961 ccaatttaat gctgtttgct gtaaataagc ttcagcttcc gtcttggctt ttagtgcttc
     4021 acctaaagct tgctgtgcta atgggctaat taaatcccat tgttctgcac acccaacact
     4081 agtaactaaa actactttcg tagttggtga atatttttca actgctttta tcaaattaat
     4141 atttgctgtt ttgtcgctac gaataccttg ttcatttttt cctcctacta ccgtaatcaa
     4201 gatttccggt tgtgtttttt gtaacaaggc gttgcaggat tccaaatcta ataaatctgc
     4261 tatttctact gctatatttc gttcgctgaa aaacgtatcc atagcatctt ttcgtaagac
     4321 ggctatcgtt ttctctctag gtaatttgtc acagattgct cgtcctaaca acccatttgc
     4381 cccacaaacc aaaatcatga ctagcatcct ccaattaatt tccgcatggc ttgaatctga
     4441 ttttctcgta attgaccttg ttcatcacgc ccaacaaaaa tcttaaacat cgcctcaccg
     4501 ctttgattga taaaagttaa cgccaccgtt ctttttccca taaatgggcg atcaagtaag
     4561 taaatagcag cacaattttc atagcgtaaa tgaccgtgaa ttcctccatc tacaccatgt
     4621 tcaaaattat aaaagcctcg cccaatttta ccttgtggca atttagcagt tacctctgca
     4681 atcgcatcag gcgtatgaat aataaaagtc acagcttcat tccaaccagc aattgcctgc
     4741 ataatttcca caaaacgctc accacctgtt tgcattacca tttctttggg cagacagtta
     4801 attaccgatt ccaagctaca ttgattcatt cctgccagca tttctaaaat ttgccctggg
     4861 ttttctttta aatttgtccg caatttattt tctaattctt tagttaatgg cgttatcatg
     4921 ttgttatcct tacttgattg aggaagtgtt aacattagtt tacgaattaa tgttggtgac
     4981 cagtaacgac cactggtatt gagatatact tgattttttt cacgcttcag taaatcaagc
     5041 gcttcccatt gttgcaataa tttttcagct ttcgcatttt gttcaaaagg cgttgtatct
     5101 aagtaaccca attccatgct atgttgtatc acaccaagca gtgctttatg ttcaccatgt
     5161 ttactcaaaa aagaaaggct tttctgatca agaggcgaat ctaagtaggt ctttaaatca
     5221 gattgtactt ggtaactcag cccaccaaaa tttccccccg caccggaacc aaatgccaaa
     5281 caaggcatat tcgatttaac caaagtatta tagcgattgc gttcacctcg atttggaaaa
     5341 gcaaagtggt tattgctcac ttgctgccaa ccttgtgctt ttaacttctc tactgcataa
     5401 gcatagtgta agctttgggt gtcaaaccct gcgggcgcag gtaattttcc atgttcaatt
     5461 aagcgattaa taggcaggaa aggataatta ttaaaggcgt aaatatctaa gcctgagatc
     5521 ggtaaactag tcgcaatttc aatatcgttt tgccagatat catctgtttg gtttggcaag
     5581 ccaaaaatta aatcagcaac aatcacagca tttaattccg ataaggcttt taaataagtt
     5641 tgagcctctt cgccactatg tttgcgccct aaacgtttgc gaatttcgga gttaaaagtc
     5701 tgaataccaa tagaaatacg gttcacgcct gcttcaatac aagccttagc cttatctata
     5761 tcaaaatggc taattctacc ctcaatagta aattcacagt cttcagcgag tggcaaataa
     5821 cgataacaag ctcgaatcaa tcgtactaaa tcttctgtat gtaacgctgt tggcgttcct
     5881 ccaccaaaat aaacggcatg aatcttacct tgtcctcgcc atagtgaaga ttcatatcga
     5941 agttcactaa tcacacgatc agtataaatc tggctataat tttccttcca agcattgcga
     6001 taaaaaccac aaaaaataca atgactagca caaaacggaa tatggaaata agctaaacca
     6061 tcctgtaata acgcatttgg tgcttgttcc agccataaag attgccattg attgcgttca
     6121 atatttttcc caccccaaat aggcataagt gcttgtcgtt ctgggaaagc atttggagag
     6181 gtttgttgag atgtccagtg tgtatctttt ataatcatta tcgaaacaaa atagtgctat
     6241 aaacaaggtc atatttataa caaaattaag ttatgaatga cttgattaaa atcaaataat
     6301 aattattttc aattaatgat tgagttatgc ttactatagt aaatagcctc tcctaaaatg
     6361 aatatagcta tggtttattg ctgtaattcc acaatacaag tcacttgact ggaatctaac
     6421 cctttaattc gccacggtgc attaaaaaac tgcttaatgt tggcattttt agggagttcc
     6481 gctaagtgca tatcttcaat cacagtggta aacctgccgg tatagtagcc taaaatcgct
     6541 cgatggcaat ttttagggtt ttccgcatca ggcactcggg aagaaggaga ccctagtgcc
     6601 aagaaatcaa tgcctactcc tttcaaattc gggaaagtat cagataaata ctgcccggca
     6661 ccggttgaaa tataggggca ttcattttga taaatagcag ggttctcggt tctatatttc
     6721 tcaaaccccg ttcgtaaaaa ggcaaaagac accttggaga gaatatcgac ataaggcaat
     6781 aaatcttctt tggtgatccc ttctgcctct cctttgggaa tttcaattaa agcgacctct
     6841 ttatggcaga aatactctat cggcaattca ttgatactta aaccgtcttt cacaaagtgg
     6901 cgaggagcgt ccatatgtgt gccgcaatga ttcggtagtt tcgtgataaa actgctaaat
     6961 ggcgtttctt cacctacgtc tgtgcagcga gtcacctcaa caacaggttc tcccggccaa
     7021 gctaaatctt taggatctaa cgggtgagac aaatacataa acatagtgag cctccttagt
     7081 ggcgtttttg ctgaagtaac cacccaatca cctcagggct gtgatacaca gcgtcccaag
     7141 tattatgagg gttattattg acaatgccgt tgtcgccggc ttgttcaaaa gtgaattcgg
     7201 tatatttcac attgggggca tttaactcat ctttgagaat gcggtagtta atgcgagaac
     7261 cttttaccgg cgagactttg tcctctttac tatggaaaaa ccaaatcggt atatgtttaa
     7321 ttgccgccaa attcgttttg gtggcattgc cgtccaccca ttcgttggtg taggcttcac
     7381 gaccgctcat taataatgct ccggcaaaga aattcgggcg agccaacaac agatttaagc
     7441 ccccttctgc acctcgagat aaaccattga tataaatgcg tgtggtatca atttgaggat
     7501 tggctttgag ggtttcatca atcattttta acacgagatt gatacgattt tccgtttgcc
     7561 agtgaatacc gcctttttgc cccttggcgg cattatcaaa tgggtcaaac acttggtcgt
     7621 attgaggagc aagcacaaaa cttggggcat aaggcagggt agcaatcgcc cctttactgg
     7681 aaagcaggtg agccaaatta tccgatccga cttgccctga gccgtgtagg aaaatcacaa
     7741 gcgggtattt ttcaccattt ttttgcaaag gcgaatagaa gcggtagttc agcttatttt
     7801 caggtttgtc taagctcact tgttttgcga caaaatcatc gagataaggg gttttgattt
     7861 tgtcgggttc agcggtttgt gtccattcag ccttgcctag agttgtgccg ctggttaatt
     7921 taagcaagcc tttttgttcc accgaataga ttaggcgatt tgcaaaaaat tcgggaattt
     7981 tgaccgcttg tactttctcg gtttcgacaa tgttgccgtt ttcatctttg gatttaaact
     8041 tcatcggtgc tttattggca tcttttacgc tataagttgc cgcattttta tctgccgtat
     8101 ccaattcaat aattacaaat ttaccggctt gcggaagtgc ggtcactttt ggctcattat
     8161 tgacataggc tttgagtacc gaacggtttt ctttctccgc actatctaat gccgttttga
     8221 cctgatacag ttggcgaaga tcgctacccg ataatacatt atcaggatat tccaatgcca
     8281 cgccgttaat tttttgacct tgagcggtga tttcggcaag cagcgtgata ttggttaatt
     8341 gattttcttg aactctatcc tccgcataaa gggcggaaga gattaatagc atacataaaa
     8401 gcgatttttt cagcataggt ttctcctaaa aaaatccacc tcataagagg cggattttaa
     8461 gcagtaacag agtgatatta aacgaataac atccaaataa tgccaacaac agcaaaggcg
     8521 attacaccga aaatagtggt taaaaacgtc caagttctta agcctaccga tacgggaaca
     8581 ttgaagaact tcgtccatac ccagaagagt gaatcattaa cgtgggataa ggtcattgaa
     8641 ccagctccga tagcacaagt gagcaatgca agctgtaatt cagtgtaccc cgctgctgcg
     8701 gctgccggag ctaaaatcgt tgtagtagta atggttgcaa ctgttgccga gccttgtgcg
     8761 gcacgcagta aagcagacag aatgaaagcc agaaccagta atggtaaacc tgtgttggct
     8821 aatacatctg ccaatgcttt acctacgcca ctggcatcta acactttacc gaacataccg
     8881 ccggcaccag tgattaaaat aatcccggca atcggtgcta acgcagaatc cagcatttct
     8941 tctaatttat gcttagtcca accgtatcta aagcctagta cataagcaga aagacaaaca
     9001 gccacaaata aggcaaacgg agagcttcct accatactga acacttcacg cacgatatgt
     9061 tctttcggca aggttgttgc cgttaccgta cctaacataa tcaggatcaa agggaaagaa
     9121 atcattgcca atactttacc gaaagaaggt aattcaatcc ctttctcttt aatcaaatct
     9181 ttttggtgct gtaaaagatc ggttgaagtc actactttta caaagtaatt tttcgtaaag
     9241 aatggaataa acttctgacc gattaaatat gccggcagtg ctgcaactaa accaattccc
     9301 aatacaaagc caatatcagc gtttaagttc tgtgcacccg ccacaattcc cgggtgaggc
     9361 ggaagcaaaa cgtgaaccaa taacatagaa agcccgatag gtagtgagta aaccaaaata
     9421 tttccaccta aacgtctcgc cacactaaaa ataatcggga tcaatacgat aaaaccaaca
     9481 tcaacgaaaa ccggtatccc gaagataaag ccagtcatac ccaatgcaaa gggagcattt
     9541 ttctcaccaa aacgctgtaa caaccaattt gctaatacgt ccgcaccgtg agatttttcg
     9601 ataattttcc ctaacattgc tcctaaacca acgatcagag caacacttcc tagagtactc
     9661 cccatccctg aaatcaaggt tgggattact ttattcattg gaatacctgc agttaaggca
     9721 actgcgatac ttactaatcc caatgctaag aatgggtgta ctttaccttt aatcataatg
     9781 accagaagag caactacgct tgctaatcca atcaaaatta acatttcatt tgacataaag
     9841 tttccttcta aatgttgtat tcacgtattt atccgctaat catcgtggtt atttatactg
     9901 ctcaaaccaa cctagtcctg aggtggtttc cgctcgtggt ttatactcgc aacctacata
     9961 gccttcatac ccgatttcat cgagtttgtt gaagatatat tggtagttaa tttccccttc
    10021 gtccggttcg tggcgatctg gcacagaggc aatttgaatg tgggcaaatt tacccttgag
    10081 tttgtcggtt aggcgagata agttgccgtc cacattctgt gcgtggaagt agtcaagctg
    10141 tacaaacaca ttgtctcggt ttaccgcttc cacaatttct agcgtatcaa actggctttt
    10201 aagcagataa ttcggtttca cttccgggct tagggcttca agcaaaacgt taatgccgtg
    10261 cggtttgaat ttatcggcgg catattgcac gttttcgata aaggtttgtt tataggcttc
    10321 acgatcttcg ccttctggca cgaccgctgc catcacgtgc acatttttac aacctaatgc
    10381 gagggcgtat tccaacgcca aatcaatgtc acgatggcta tcggcttcac gccccggaat
    10441 ggcggaaacg ccccattccc cttgagcaac atcgccggct gcggtattga ataacacctg
    10501 ttttaagcca tattgctcaa gtttggcttt cagttctgct gccggataat cgtaaggcca
    10561 taaatactct acatatttaa atccagcgtt ggcagccgct tcaaaacgat ctaaaaacgg
    10621 cacttcgtta aacatcatcg tcaggtttgc tgcaaattta ggcattttat tttcctctgt
    10681 ttttcagatc attgacttcg tcatcggtga gataacggac tttttgacct tgtagaataa
    10741 aatggagttt ggcggtttct tccaactctt cggtattatc caccgcatca cgcaggtctg
    10801 aacctgtaac tacaaccccg tgatttgcca gtaaaaaggc tttaccgtcc aaggctcgtt
    10861 cccccaactc tcgggcaatg ttgatgtcgc ctgggcggta gtaaggaatg accggcaatt
    10921 ttcccacccg catcacatag taaggggtga aagcacgcat agcgttgtcg gtatccaaat
    10981 tctccaagca agaaagtgcg gttaaataag tggaatgtag atgcacaatc gcttggcaag
    11041 ccaggttttt ctgatacatc gctaagtgga atactgattc ttttgaaggc ttatctcctg
    11101 aaagaacatt accctgcata tccaatacgg aaaggcggtc tgcctctaaa cgcccgagcg
    11161 atgagccggt tggggtaact aaaatgcgat tttcatctaa acgcacagag aggttgcccg
    11221 ctccgccaac ggtataaccc cgctcaaaca gcgagcgggc aagattgacc atttcaatct
    11281 tttgttcttg ttctgtcata caaacatctc ctgagcaaaa ctgaagaaat cttctttacc
    11341 gaagttaccc gatttcaatg caagaaaaat cggtttttcg accgcttgta accacggcac
    11401 gcccggtgca atttgtttgc cgatgtggaa accgctgaat ccaagtttct gaaccacaat
    11461 actcgaagtt tcaccgcctg cggtaataaa gttagttacg cctgcggctt gtaatttttc
    11521 ggctaatttg gcaaaggtat tttcaatggc gtggctggct ttatcgccgc cgaattgggt
    11581 ttgaatctct ttcaattttt cagggggaac ggtggcataa accattgggg caaagtcacc
    11641 gtctaggttt gcaatcaccc attgataaag cgtttctaca tagttcggat cctgaatggc
    11701 tttttccgct tctaagaaat aacttggggc tttttgctgg taagccgcca cttgggtatt
    11761 ggtcattacc gagcaagaac ccgaaagcac aacggttttg cctttgcttg gtacaaacgc
    11821 attcgtgcct tttttgccgc ctgaacggta agccgccata taagccccta agcctgaacc
    11881 gcctgtgact aattgaaaat cggcaaccgc ttcggcaagc accgcaagtt gtgaattatc
    11941 ggcagcatct actaccgcat atttgtagcc ttgctgtttc aattcagcaa aacgggcttt
    12001 cacacgctct gccccttgaa tcacatcagc ataggcaacc aagccggttt tgccttgaga
    12061 ttgtgcatcc attaaacgca ccaagttggc atctttcatc ggggtaatcg ggtggttacg
    12121 cataccagat tcattcagta gcacatcgcc cacaaacaaa tagccgttga aaatggttct
    12181 gccattgaca ggcagagccg gtgtgactac ggtaaaatcg gtatctaatg ccgccattag
    12241 ggcatcggtt accggcccga tattgccttt ctccgtgctg tcaaaagtgg aacaatattt
    12301 aaagtagaat tgcgagcaac cattttgttt cagccaatct aaggcttgta atgattgttg
    12361 gattgcttct tccacagggt tagagcgaga tttcaggcta atcacaatcg catcaacttg
    12421 actgttaagt gtagttttcg gcacaccatt catttgcacc gtttttagcc cgttttcggt
    12481 taagaagctg gcaatatcgc tcgcaccagt aaaatcatct gcaataatgc ctaacattag
    12541 ttgtctccct tttttggtag ctcaataccg ctgaaaattt tgatgaccgc actatcatct
    12601 tctttgccgt aacctgcatt gcttgcttca gtaaacattg agtacgctgt gcttgctaaa
    12661 tgaagtggga agcgtaatga tttcgctgtg tcattcacta agcctaaatc tttgacgaaa
    12721 atatctacca ttgaaagcgg ggtgtagtcg ccatcaacaa cgtgtttcat acggttttca
    12781 aacatccaag agttacctgc tgcattagtt accacatcgt acatcagatc taacggaatg
    12841 ccggcacgag cggctaatgc catcgcttct gcacctgcgg caatatgcac accagctaat
    12901 aattggtgga taattttcac cgttgcacct aagccgatct cttcgccgat gttataaact
    12961 tttcctgcaa cagcatctaa aacaggtttt agtttctcaa acgcttcact tgagcctgat
    13021 gccattacgg tcatttcacc ttgtgccgct tttgctgccc caccggaaac cggagcatct
    13081 aacatcacca agcctaaatc tgttaatttc tgtgaaatat cttttgcacc ttgtgcagaa
    13141 atggtggaag acaccatcac caccgtgcct tgtttcaaat gttgggctaa gccgttttca
    13201 ccgaataaca cagcattcac ttggttggca ttaaccacca ataacaacac cgcatctaat
    13261 tcagcggcaa aatcgaccgc acttgtgccg actttcttag cacctgctgc ttttaaggtt
    13321 tccagataat tcgggttaag atccacaccg tatgtgtcta atccggcagc gatacaagat
    13381 tttgctgctc ccatacccat tgctcctaag ccgatgacgg ctacgctata tttttgactc
    13441 attgtttatt ccctcaaata aattaatgaa cttgatggcg gtatattaac caatagaaaa
    13501 gataaaatct gtgatgaaga tcacaaatat taacaataaa tgtgaatttt tgtgatttta
    13561 attttggaag ctaaatctga tataatctct tttaacaatt aaaataggtt aaaaaatgat
    13621 ccccttagag agacaacgag aaatactacg acttattaat aataatggtg tcgcaagtat
    13681 taattttctg gcagaacagc taaaagtatc acatatgaca attcgccgtg atattcaaaa
    13741 gctggaagag caaggtaagg tatccctagt atctggtgga gttcaatctc ttgaacgatt
    13801 aatcatggaa ttaccacacg atgataaatc aatgttattc caagagcaga aatcagctat
    13861 aggatcttta gcatcacagc taattccctc aaacacaact atttatcttg atgctggaac
    13921 tactacatta gaaatagctc atcggataat cgatagaaat gacttactag ttgtaacaaa
    13981 cgattttgtt attgctcact atcttagtaa ttcaggaaaa tgtgatttaa ttcatatagg
    14041 aggaagtgtc tgtaaagaaa acctatcgac aactggttca cttgcagcag aatttctgaa
    14101 aaacctatca atcgatatgg catttatatc tacttcatcc tggaatttaa aaggtctaac
    14161 aacgccgaac gaaaataaaa tccccgttaa aaaagcaatt ttagaggcat caaagcaaaa
    14221 cattttagtg acagactctt caaaatatgg aaaagtcgca actttttttg tttgccctct
    14281 tactgtattt gaccagatta tttgtgataa aggattattt gagaatgtac gagacgcact
    14341 gaaagggcta agtattaaat tagatttagg ataatcttct taccaatttt ttctgagaac
    14401 gacaagcaat caaaaagtat tatttttgca aataagatat taatagggaa agttaacaat
    14461 cctagcaacg gattgttaac tgattttcgg ctggtactaa taaaatagga atattaacgt
    14521 ttaccaaaca tactgcctaa gccaccgaga ccgcctagac caccacccat cataccttta
    14581 acaccacgca tcatttttgc cattccgcct ttacgcattt ttttcatcat acgttgcata
    14641 tcatcaaact gcttgagcag cttatttaca tcttgcacct gtgttccgga gccaagtgcg
    14701 atacggcggc ggcgtgagcc tttaataata tccggatttg cccgctcttt taaggtcata
    14761 gaattgatga tggcttccat tttcatgaac attttatcat caacctgact tttaacgtgg
    14821 tcaggcaggt ttttcgcccc cggtagttta tcgagcatcg acatcattcc acccattttt
    14881 ttcatttcga taagctgctc acggaaatct tccaaggtaa attcatcgcc ttttttgaat
    14941 ttggcggcaa ttttttccgc tttttcttga tcaaccgagc gttgaatatc ttcaattaaa
    15001 gacattacat cgcccatacc taaaatgcgg gaagccacac ggtcagggtg gaacggctca
    15061 agggcatcag ttttttcacc cacacctaag aatttaatcg gtttaccggt gatttggcga
    15121 atagaaagtg ccgcaccgcc tcgggcatca ccatcgactt tggtaagaat aacaccggta
    15181 agcggcaacg cttcgttaaa ggcttttgcg gtgttggcgg cgtcttgacc ggtcatcgca
    15241 tcaaccgtaa atagcgtttc aatcggattc agcacagcgt ggatttgttg gatctcttcc
    15301 atcatttcgc catcaacgtg taaacgaccg gcagtatcca caatcaatac atcaaagaaa
    15361 ttaagcttag cgtgcttcag tgccgcttct gcaatcgcta ccggtttttg gctggtttcg
    15421 gtcgggaaga agtccacttt taacgtttca gcaagggtct gtagctgctt gatagcggca
    15481 gggcggtaaa catcggcaga aactaccaac acctttttct tgtggcgttc tttcaggaat
    15541 tttgccaatt tgccgacaga ggtggtttta cccgcacctt gcaaacctgc cattaaaatt
    15601 accgcaggcg gttgggccgc aagattcagc tcctcgttgg cttctcccat tgccgtttct
    15661 aattcgcttt gtacgatttt taagaactct tgccccggcg tgaggctttt gtttacctca
    15721 acccctaacg cacgctcttt gaccttgttg atgaaatcac gcacaacagg gagggcaaca
    15781 tccgcctcca acaatgccat acgaacttca cgcagggtat ctttaatatt atcttccgtt
    15841 aggcgacctt tgcccgtaat gttacgcagc gttttagaca gtctgtcaga gaggttttca
    15901 aacatatcag ctttctcaat ttgcaaaatt ttaggaaaaa agtaccgctt gtgagcggaa
    15961 agttgccgcc attatacaaa aaaaagcgtt acggttatag ggaaataaaa aaacggcata
    16021 gcgattaaac tatgccgtaa aatttgacga tattcgagga atcattattt agttatattg
    16081 tgcgatcata atactccgaa ttgcgtataa aagtgaagtg atattttcaa atttatgtta
    16141 gaaatgagag gtaaattaca aaattaaagg ggatattatt gtttgaaaaa ataagagcga
    16201 aaagaaaacc ctgatgattg ctcatcaggg cttc
//
LOCUS       DANJWC010000043        15768 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.043, whole genome shotgun sequence.
ACCESSION   DANJWC010000043 DANJWC010000000
VERSION     DANJWC010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15768
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.043"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            486..833
                     /locus_tag="PWX02_000996"
     CDS             486..833
                     /locus_tag="PWX02_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729024.1"
                     /translation="MFREEAIEYQSKKWKSTAVLFSRVPALVVFSISFFIFTAFILFI
                     VFGNYTRRETVVGELVMQSHPILLSAPKSGYISENYIQPHQQVKKRAIRWLKLRWIEL
                     RIAKILMLILFFR"
     gene            830..1747
                     /locus_tag="PWX02_000997"
     CDS             830..1747
                     /locus_tag="PWX02_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HDL1729025.1"
                     /translation="MKSQIQATEQAITALYKNKSETINSLEKQIKNSQKIYQDKQSYL
                     VEIEKSMNDYAELVKRYEKLLKVGHSSHDEVNIQKSRYFQQKSLFNELKQELIQLKST
                     QLSLENEIETRKTEFNNQIIRYEMQKSDLNIRLMEFESVSDIIVNASLDGKIESTSVT
                     VGQIIKENDPLAQILPQNKGNYQLVMWVPNSAISFIKQGDEVNIRYEAFPFEKFGQFK
                     GKITSISTLPASLQELSFYKNLPVNLEQGIPLYKILIELADQNVKYNDTSLYFMSGMK
                     AEATLFLEKRKLYEWIFFPMYQLRKNMEQ"
     gene            1747..2538
                     /locus_tag="PWX02_000998"
     CDS             1747..2538
                     /locus_tag="PWX02_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HDL1729026.1"
                     /translation="MTGIKQLKFDFRRRIPVILQTETAECGLACLAMMLGYYDKNTNL
                     FELRSQYGTSSRGVTLHTLISIANDCGLITRPLSLELDEVPQLRLPCILHWDFNHFVV
                     LTHASEKQFIIHDPAFGKRKLSRAEFSNHFTGVALEVWSEVKFEKSNNENTISLYETL
                     KHISGIKGALVKIFALSMLIELIALLMPIGTQLVMDHVMQAKDQSLLLIICIGLFLFT
                     LFRTMVSMFRAWVSLKMNYLIDFQWTASFFSHLLKLPLDFFENAK"
     gene            2916..3833
                     /locus_tag="PWX02_000999"
                     /pseudo
     CDS             2916..3833
                     /locus_tag="PWX02_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167835.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(3835..4503)
                     /locus_tag="PWX02_001000"
     CDS             complement(3835..4503)
                     /locus_tag="PWX02_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDL1729027.1"
                     /translation="MKYLYALIFSLFFSSVYAAERQLQCKVVGISDGDTLTCLVNKTP
                     LKIRLQHIDAPEQAQPYGNKAKQALAQFVFQQNITAIVSGYDRYQRLLATLYLNDKNI
                     NLALVENGMAWAYTTKREYIEAQDQAQQAKIGLWQDKFPINPSQWRKTHTSTLPQAVK
                     KPENIANQPPTIDCQIKLSCNQIGNYAQAKRYFQQCGWKELDGNNDSIPCNKLYRQEQ
                     KNRN"
     gene            complement(4547..5914)
                     /locus_tag="PWX02_001001"
     CDS             complement(4547..5914)
                     /locus_tag="PWX02_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDL1729028.1"
                     /translation="MSIDTILNQINSFVWGPPLLILISGVGLYLTFSLKGIQLSQLGQ
                     AFRYMFKPEKGKGQSGDISAFAALSTALAATIGTGNIVGVATAIHSGGPGALFWMWLI
                     ALFGMATKYAEGLLAIKYRTKDRAGFVAGGPMYYIELGMGKKWRWLAKAFAFFGVLVA
                     LLGIGTFPQVNGITHALESTFDVPIIFSAIILTLIVTAIILGGVQRISKIATIVVPFM
                     AIGYVIAALSVILLNMEKVPQAIITIVHAAFNPQAALGGAVGFTVMQAIQLGVARGIF
                     SNESGLGSAPIAAAAAQTKEPVRQGLISMTGTFLDTIIVCTMTGLVIVISGAWAGDVK
                     GAELTTTAFTQGLNSSFGAIIVTVGLVFFAFTTILGWCYYGERCFVYLTRGRTQYIKL
                     YRLLFIALIASAPFLQLDTIWTLADIVNGLMAFPNLIALIALRKVIISETKDFFARLK
                     TGKAE"
     regulatory      complement(6033..6128)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(6299..7351)
                     /locus_tag="PWX02_001002"
     CDS             complement(6299..7351)
                     /locus_tag="PWX02_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL1729029.1"
                     /translation="MTHSTKTLVAIASIIIILAGIKLSNEIVVPFLLSLFIAIICSPM
                     IKFMTKRKVPLWLAITILLIVFILGFSFLAGIINNTLQEFTNAIPQYKIQLSSRIQVI
                     LELAHRWHIPLTLSQEEIMSKFDPSSIMNLVSKLLMGLSGALSNIFVIFLVVIFMLLE
                     MPVAKYKLALALSPDDDLSNEKEYINQVLEGVIGYLGVKTFVSLLTGGLVWILLEILG
                     VEYAILWAILTFLLNYVPNIGSILAGIPIVIQALVLNGFSIGLGTLIGIVVINMVVGN
                     ALEPKLMGKKLGLSTLVVFLSLLFWGWLLGTVGMLLSVPLTMACKVALESNKATQKYA
                     VLFSDLEEVPKEQKYR"
     gene            complement(7398..7823)
                     /gene="ruvX"
                     /locus_tag="PWX02_001003"
     CDS             complement(7398..7823)
                     /gene="ruvX"
                     /locus_tag="PWX02_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDL1729030.1"
                     /translation="MTETTTKTVIAFDFGTNSIGCAVGQSITGTAQGLTTFKAQDGIP
                     NWEEIGKLLQDWKPDMLVVGLPLNMDGTEQPLTQRARKFANRLNGRFNLPVELQDERL
                     TTVEAKAEIFSRGGYKALKKDKVDSISACLILESWFENH"
     gene            complement(7823..8383)
                     /locus_tag="PWX02_001004"
     CDS             complement(7823..8383)
                     /locus_tag="PWX02_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HDL1729031.1"
                     /translation="MLENLQGKFLIATPEMDDDYFERTVVYICEHNPQGAMGVVINTP
                     TDLSVLELITRMDFQMANQRDYKKDQMVLSGGPVNQDRGFIIHTKTANDFIHTYPITD
                     KITLTTSGDVLDSFGTLASPEKFIVCLGCSTWRGSQLEQEIAQNYWIVSDANEKTLFE
                     TGYLERWAEAYEMMGLSSIPSRAGRA"
     gene            complement(8383..9114)
                     /gene="rsmE"
                     /locus_tag="PWX02_001005"
     CDS             complement(8383..9114)
                     /gene="rsmE"
                     /locus_tag="PWX02_001005"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDL1729032.1"
                     /translation="MRIPRIYHPQPLAGLQSCVLSEDAANHVGRVLRMKQGEQIILFD
                     GSNHVFHATLQAVEKKQIIAKIDSSELDDRESNLPIHLGQVISRGDRMEFTIQKSVEL
                     GVKTITPLWSERCGVKLNDERQDKKLQQWQKIAIAACEQCGRNEIPEIRPIMKLTDWC
                     KEQDDMLKLNLHPRAKYTIRELPNVPKAGVRLLIGSEGGLSAEEIAMTESEGFTEVLL
                     GKRVLRTETASLAAITALQVCFGDI"
     gene            complement(9174..9464)
                     /locus_tag="PWX02_001006"
     CDS             complement(9174..9464)
                     /locus_tag="PWX02_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL1729033.1"
                     /translation="MPKKLHNIYYTEEALIDLENIAISVSEFTGYASSGIRILEELEN
                     SINNLAIFPTMGVKGAIINTRELYHNGYRIVCEVKENEISIITIVHCSRLYP"
     gene            complement(9445..9618)
                     /locus_tag="PWX02_001007"
     CDS             complement(9445..9618)
                     /locus_tag="PWX02_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729034.1"
                     /translation="MNNPYHNVSQEYLDYLAKKVKQGKEDIKNGRIYSPEEVRKRVIA
                     AAFRGAENAKKVA"
     gene            complement(9790..10377)
                     /locus_tag="PWX02_001008"
     CDS             complement(9790..10377)
                     /locus_tag="PWX02_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729035.1"
                     /translation="MKRSKKTLVLATTFVAALLAGCQSQSNTLTFSTPTPTAVFNTNN
                     QTAAVNVMTQDLRTSREIASYTKNGEVIRLTAVPEVAAMFQQAVQQDLNAKGFQIVQG
                     AANANVVVNIRKFFATVEQGNLRYKADAQVSVKVAVQGSKGNFSKNFNATRGYEGAFG
                     ASNDEIKKVLDQAYSDVVKAIYNDNEIANAIHQFK"
     gene            10469..10780
                     /locus_tag="PWX02_001009"
     CDS             10469..10780
                     /locus_tag="PWX02_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HDL1729036.1"
                     /translation="MSVEQTIIQKIKTEFQPIFFNVENESYMHSSGRGAESHFKLTLV
                     SDKFEGQRTVARHRAVYACLAEELANGVHALALHLYTQGEWEATGGLVPKSPNCLGVG
                     Q"
     gene            10872..10961
                     /locus_tag="PWX02_001010"
     tRNA            10872..10961
                     /locus_tag="PWX02_001010"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10906..10908,aa:Ser,seq:gga)
     gene            complement(11137..11511)
                     /locus_tag="PWX02_001011"
     CDS             complement(11137..11511)
                     /locus_tag="PWX02_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE N-terminal
                     domain-containing protein"
                     /protein_id="HDL1729037.1"
                     /translation="MKKIALIGFTTAMLGACSLLPQKSVSGTYAGELPCADCEKIQAQ
                     LTLNADKTYQYDTVYFKDKKEYTYRDKGTYSWEPNKPNVIRLEKASGNLAFQVSEQHV
                     ELCDPNGNTVKNSNNYKLGKVR"
     gene            complement(11536..11838)
                     /locus_tag="PWX02_001012"
     CDS             complement(11536..11838)
                     /locus_tag="PWX02_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HDL1729038.1"
                     /translation="MKNSAPKKINDILQNSSLSRIVEKANFINDLNQKIQKRWPESYR
                     GLYRVVNLVDNQLIVSVKNATVRQGLILQQNMLLKQIQTDLPEITQLEFRVNPSVT"
     gene            11959..12279
                     /locus_tag="PWX02_001013"
     CDS             11959..12279
                     /locus_tag="PWX02_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2547 family protein"
                     /protein_id="HDL1729039.1"
                     /translation="MSLFLKHFHRTPFWSQLFLGMVAILALPEIQVAYANESEQETVL
                     NQSFLQYAQSANDIEQQSLFIAELIHKIVEIKPQAVHFCEFFANSYRLDRQNAYPIRA
                     GPQT"
     gene            12338..15064
                     /gene="secA"
                     /locus_tag="PWX02_001014"
     CDS             12338..15064
                     /gene="secA"
                     /locus_tag="PWX02_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDL1729040.1"
                     /translation="MISKLLTSIFGSSNDRTLKRLRKRVAQINKLEPTFEQLSDEALK
                     DKTAEFKQRLADGASLDSLLPEAFATVREASKRVLGMRPFDVQLIGGMVLTGRNIAEM
                     RTGEGKTLTATLSCYLNALTGKGVHVVTVNDYLARRDAETNRPLFEFLGLTVGVNIPG
                     LPSDAKRAAYQADITYSTNSELGFDYLRDNLAHDKNERFQRELHYALVDEVDSILIDE
                     ARTPLIISGPAEDATQIYQAVDQIIPHLIAQDKEDSEEYTGEGDFTLDLKNKQAHLTE
                     RGMVKVEDFLTQMGLMEEGESLYHPARISLLHHVSAALRAHKLFERNVDYIVKDGEIV
                     IIDEHTGRTMAGRRWSDGLHQAIEAKERVAIQGENQTVASITYQNYFRLYEKLAGMTG
                     TADTEAFEFQQIYGLNTVVIPTNRPVIRDDRTDLMFKSEAEKFAAIITEIRDCIEREQ
                     PVLVGTASVEKSELLSAELTKAGIAHNVLNAKFHAQEAEIVAEAGAPGAVTIATNMAG
                     RGTDIVLGGNWKMEVAKLENPTQEQIDAIKAAWQERYDIVMQAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDPGSSRFYLSLDDALMRIYLNEGKLNMMRKAFTEEGEAMESKLLT
                     KVIASAQAKVEAHNFDGRKHLLQYDDVANEQRKVIYEQRNHLLETDNIASMIESIRQD
                     VFESTINQYIPPQSIEEMWNVPGLEERLRREFGLDLPISHWLETEKDLHEETLRERIL
                     NIAIAEYNAKEEIVGAEVMRNFEKGVMLQNLDELWKEHLASMDYLRKGIHLRGYAQKD
                     PKQEYKKESFAMFTNMLDTLKLNVISILSRIQVRSKEEVEEAQRQQAEMDAASTPVTQ
                     ATDSAAGSREENSAESLSEEELANLNIGRNDPCPCGSGKKYKHCHGNKARYS"
     gene            15151..15549
                     /gene="mutT"
                     /locus_tag="PWX02_001015"
     CDS             15151..15549
                     /gene="mutT"
                     /locus_tag="PWX02_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HDL1729041.1"
                     /translation="MSKPIIQVSAGIIRNEFGQIYLTQRLEGQDFAQSLEFPGGKVDP
                     NETPEEALKRELEEEIGIQVLSAFPYENFCFEYPTKIIEFFFYLVEEWVNEPYGREGQ
                     EGFWIAQSDLDEGAFPPANAQLIKRLKEHA"
BASE COUNT         4748 a         2900 c         3185 g         4935 t
ORIGIN      
        1 gattcttagg acgttattac ctttaataat gttatctgct tgtgctgact atcaagaata
       61 tgataaaaag gatagtaaat gttattattc ttcctatgga agtggagagg caaaagattc
      121 tgttaggaaa agaatagagt ataatagatg tttaagagag aataagcctt atttcataag
      181 tggcaatcat tgaataatat atcttgtctg tatagatgta agattaatta atgtatgtaa
      241 ctaaatttta ttgcgagtgt tttatgagat ttgttactgt aatttatatt gcgtttattt
      301 attttatgat ttacagattc gtttatccat tattaccatt cgagtgtaat acagaagttt
      361 atgatatagt gttgttttgc tttttgttta cttattctct tcttaagaag taacatgttt
      421 ataagattag cttttaatgt atataaaata aatgtgctaa tgtagtagtg taaaattggt
      481 attatatgtt cagagaagaa gctattgaat atcaatcgaa aaaatggaaa agcacagctg
      541 tactatttag cagagttcca gctttggttg tcttttccat ttctttcttt atctttactg
      601 cctttattct atttattgtg tttggtaatt atacccgccg tgaaacggtt gtgggtgaat
      661 tagtgatgca atctcatccg attttattat ctgccccaaa atccggctat atatccgaaa
      721 attatattca accacatcag caggtaaaaa aaagggcgat ccgctggtta aaattacgct
      781 ggatagaatt acgcatagcg aaaatattaa tgttaattct attctttcgt tgaaatcaca
      841 aattcaagca acagaacaag cgataactgc tttatataag aataaatcag aaactattaa
      901 tagccttgaa aagcagatca aaaacagtca aaaaatttat caagataagc agagttattt
      961 agtggaaatt gaaaaaagta tgaatgacta tgcggagttg gttaaacgct atgagaaatt
     1021 attaaaagta gggcattcca gccacgatga agtgaatatt cagaaaagcc gttatttcca
     1081 acaaaaatca ctgtttaatg agctaaagca agaattaatt caattaaaat ccacccaact
     1141 tagcttggaa aatgaaattg aaacccgtaa aaccgaattt aataatcaga ttattcgcta
     1201 tgaaatgcaa aaaagcgatt taaacattcg tttaatggaa tttgaatctg tttcagatat
     1261 tattgttaat gcctctttag acggaaaaat tgaaagtacc agcgtaacag ttgggcaaat
     1321 tattaaagaa aatgacccgc ttgcacaaat tctgccacag aataaaggaa attatcagct
     1381 agtgatgtgg gtaccgaata gtgccatttc atttattaaa caaggcgatg aggtgaatat
     1441 tcgctatgag gcttttccgt ttgaaaaatt cgggcagttt aagggcaaaa ttacgtcaat
     1501 ttcaacctta ccagcttctt tgcaagaact tagtttttat aaaaatctcc ctgtaaattt
     1561 agagcaaggt attccgcttt ataaaattct gattgagctt gccgatcaaa acgtaaaata
     1621 taatgatacc tccctttatt ttatgagtgg aatgaaagcc gaagccacgt tatttttaga
     1681 aaaacgtaaa ctgtatgagt ggatattttt ccctatgtat caattacgca aaaatatgga
     1741 gcaataatga caggaattaa gcagttaaaa tttgattttc ggagaagaat acctgttatt
     1801 ttgcaaactg aaacagctga atgtggctta gcctgtttag cgatgatgtt gggctattac
     1861 gataaaaaca ccaatttatt tgaattgcga agtcaatatg gtacttcctc tcgtggcgta
     1921 acgctgcata ctttaatttc tattgcaaat gactgtggtt taataactcg ccctctttct
     1981 ctagagttag atgaagtacc acaacttcgt ctgccttgta ttcttcattg ggattttaat
     2041 cattttgtag tattaactca cgcaagtgaa aaacagttta ttatccacga tcctgctttc
     2101 ggtaaacgta aattatcccg tgctgaattt tccaaccatt ttaccggagt cgctttagaa
     2161 gtttggtctg aagttaaatt tgagaaaagc aacaatgaaa ataccattag tttgtatgaa
     2221 accttaaagc atatttctgg cataaaaggt gcattagtga aaatttttgc cctttcaatg
     2281 ctcattgaac tcattgcctt attgatgcca attggaacac agttagtgat ggatcacgtt
     2341 atgcaagcga aagatcaatc attactttta attatctgta ttgggctatt tttatttacc
     2401 ctttttcgca caatggtcag tatgtttagg gcttgggttt ccttgaaaat gaattattta
     2461 attgattttc aatggacagc cagtttcttt tctcatctct taaaattacc gcttgatttc
     2521 tttgaaaacg ccaagtaggt gatattcatt cccgatttag ttcattaaaa accattcaga
     2581 aaactctaac ttcaagcatt attaccacga ttattgatac gattatgatc atcagtttaa
     2641 ttattatgat ggtgttatat ggcggttggc tcttttgggt agtattagct ttttccttta
     2701 tctattttat tcttcgaatg gttacttatt tagtttatcg tcaactgaat gaagagcaga
     2761 ttattaaagg agcaaaagcc cattcgcatt ttatggaaac tttatatggt attacaacgt
     2821 taaaatcatt aggattggaa aataaacgac aagaacagtg gatgtcgctc aatgcggatg
     2881 cttttaacac atcaattaag gtaactaaat ttgatatgct gttttccggt attcatacct
     2941 ttatttctac catcgaacag attttaattc tttggattgg ggcgacaatg gtgattgaaa
     3001 atgtgatgac attaggaatg tttgtcgctt ttaatgccta tcgaggctct ttctcttcac
     3061 gaatggggaa tttaattgat attattttta gcctaaaaat tctttcatta catagagagc
     3121 ggattgcaga cattgcttta aatgaagtcg aagatgaaag taaatatcaa gaactcagat
     3181 ttgaacataa ccaaacagat atagaagtta aagacctttg tttcaaatac gatccttttt
     3241 ctaaaaatgt gcttgaaaat gtcaatttag tgatcagaag tggggaaagt gttgctatta
     3301 ccgcaccatc aggttatggc aaaacaacgt tattaaaatt aattgcaggt ttattaaaac
     3361 caacctcagg ttctgtgtat tttaacggca ttgatattta tcagttagga ttaagtcaat
     3421 atcggagcca aattgcttgc gtattacagg aagataaatt cttttctggt tccattttag
     3481 agaatattgt tagttttgaa agtaattatg atcgagaatg ggctatcgaa tgtagcaaac
     3541 ttgcgaatat tcacaatgat attatggcaa tgccgatgaa ttatgaaacc ttattaggag
     3601 aattaggaaa taatttgtct ggcggacaaa gataacgatt gtttattgct cgagctttat
     3661 ataaaaagcc taaaattctt ttgatggatg aggcgactag ccatttagat gaagaaaatg
     3721 agaagcttgt caatcaagct atttcacgaa tgaagataac aaggattatt attgctcata
     3781 gacaatcgac tattcagtcg gcggatagaa ttattgccct gaatgacagg taaattaatt
     3841 cctatttttc tgttcttgcc gataaagttt attgcacgga atgctgtcgt tgttgccgtc
     3901 tagctctttc cagccgcatt gttgaaagta gcgttttgcc tgtgcgtaat tgccgatttg
     3961 gttgcagctt aatttaattt ggcaatcaat cgttggtggc tggtttgcaa tattttccgg
     4021 ttttttgacc gcttgtggta aggttgaggt gtgtgttttt cgccattggc tcgggttaat
     4081 cgggaatttg tcctgccata agccgatttt agcttgctgt gcttgatctt gtgcttcgat
     4141 atattcccgc tttgtggtgt aagcccacgc cataccgttt tccactaagg ctaagttgat
     4201 gtttttatcg tttaagtaga gggtggcaag taatcgttgg taacgatcat agcctgaaac
     4261 tatggcagta atgttttgtt gaaacacgaa ttgagccaga gcttgtttgg ctttgttgcc
     4321 gtaaggctgc gcttgttcgg gggcatcaat atgttgtagc cttattttta gtggtgtttt
     4381 attgaccaaa caagtgaggg tgtcgccatc gctaatgcca acgactttgc attgtagttg
     4441 gcgttcggcg gcatataccg atgagaaaaa taagctaaaa atcagggcgt aaagatattt
     4501 cattattaaa aaagagaggg ctaataagcc ctcattctat cattttttat tcggctttac
     4561 ctgttttaag gcgagcgaag aaatcttttg tttcactaat aatcactttg cgtaacgcaa
     4621 tcaatgcgat taaattcggg aatgccatta agccgttcac gatgtctgcc agcgtccaaa
     4681 ttgtatcgag ttgtaaaaat ggagcagagg cgatgagtgc aataaacagc aagcggtata
     4741 atttaatgta ttgggtgcga cctcgagtga gatagacaaa acagcgttca ccgtagtagc
     4801 accagcctaa aatggtggta aaggcaaaga agactaaacc aactgtcaca attatcgcac
     4861 cgaatgagct gtttaaacct tgagtaaaag cggttgtggt gagttctgcg ccttttacat
     4921 cgcctgccca agcccctgaa atcacaatta ctaagccggt cattgtgcag acgataatag
     4981 tgtctaagaa cgtgccggtc attgaaatta ggccttgacg taccggctct ttggtttggg
     5041 cggcagcggc ggcgatgggg gcagagccta atcctgattc attcgagaaa ataccacgtg
     5101 caacccctaa ttgaatcgct tgcatgacgg tgaatccgac tgctccgcct aatgccgctt
     5161 gtgggttgaa agctgcgtgt acaatagtaa taattgcttg tgggactttt tccatattca
     5221 gtagaatgac ggataaagcg gcgatgacat acccaatagc cataaacggt acaacaatgg
     5281 ttgcaatttt agaaatccgc tgcacgccac ctaaaataat agcggtaacg ataagggtta
     5341 aaatgatcgc actgaagata atcggcacat caaaggtgct ttctaaggcg tgggtaatgc
     5401 cattcacttg ggggaacgtg ccaataccaa gcaaagcgac caatacgcca aaaaacgcaa
     5461 aggctttagc aagccaacgc catttttttc ccattcccag ttcgatatag tacatcggac
     5521 caccagccac aaatccggct ctatctttag ttcgatattt aattgccaat aagccttcgg
     5581 catatttcgt agccataccg aataaggcaa tcagccacat ccaaaataat gctccaggcc
     5641 cgcccgaatg aatagcggtt gccacgccga caatattccc cgtgccgata gtagctgcta
     5701 gggcagtgga aagggctgca aaagcggaaa tatcgcccga ttgccctttg cctttttccg
     5761 gtttgaacat atagcgaaag gcttgcccta gttggcttaa ttgaatacct ttgagtgaaa
     5821 aggttaaata taaccctacg cctgaaatta aaatcaggag tggcgggccc caaacaaaac
     5881 tgttgatttg attaagaata gtgtcgatag acatttgatg ttcctcgtaa aaactgctta
     5941 caaggaaaga aaaggaatgc aagagacaag cggtcaaaaa acggaaattt tttgcaaaga
     6001 attagcaaaa aaacaccgct tgtatcgtat aacttttctc ccctgtcctt ttgcctgagc
     6061 gtttcacaca ttgctgtatt ttcgccttcg gcggcggatt ttaatccgct ctctccaagg
     6121 gttcgtccag taacagtccc cgcagtgttc actgcacctg aaagagttta cctcgtcggt
     6181 gaaatgttaa ttcatttctc tccagctacc ttcatccgaa caatctaatt tattttataa
     6241 tgtaaaataa aaaagatttg ccaaattcta gcaactcttt tgcttccgtc aagtgaattt
     6301 aacgatattt ttgttctttc ggtacttctt ctaaatcgct aaataatacg gcatattttt
     6361 gggtggcttt attggattct aatgccactt tacacgccat cgtaagtgga acagaaagta
     6421 acattcctac ggtgccaagt aaccaccccc aaaatagaag tgagaggaac actacaaggg
     6481 tagaaagccc gagtttcttg cccattaatt taggttcaag cgcattaccc acaaccatat
     6541 taatcacgac aatgccgatt aaagtgccta atcctataga aaagccattg agtaccaagg
     6601 cttgaatcac aattggaatt cccgccaaaa tagagccgat attcgggacg tagttaagca
     6661 ggaaggttag aattgcccat aaaatcgcat attctacgcc tagaatttct agtaagatcc
     6721 aaactaaccc tccggtgagt aggctcacaa aggttttaac tccaaggtaa ccaatcaccc
     6781 cttcaagtac ttggttgata tactcttttt cgttgcttaa atcatcatct gggctaagtg
     6841 cgagggctaa tttatatttg gcaaccggca tttccaatag cataaaaatg acgactaaga
     6901 aaatcacaaa aatattcgat aatgcaccgg atagccccat tagcagtttg ctgaccaaat
     6961 tcattattga gcttgggtca aatttgctca taatctcttc ttgtgagagg gtaagtggaa
     7021 tatgccatct atgggcgagt tctagaatca cctggattct agatgaaagc tgaattttgt
     7081 attgcggaat agcattagta aattcttgca aggtgttatt aataatgcct gctaagaaac
     7141 tgaatcctaa gataaaaaca ataagtaaaa tggtaatggc taaccataat ggcactttac
     7201 gcttagtcat aaatttgatc attggtgaac aaataatggc aataaatagc gaaagtaaaa
     7261 atggcacaac aatttcattg gataatttaa tccctgccag aataatgata atggaggcta
     7321 tcgccacgag agttttggtg gaatgtgtca taatttttcc ttttacaagc ggttaaaaat
     7381 cagcaatttt ttacaaatta gtgattctca aaccaacttt ctaaaattaa acaagcggaa
     7441 atggaatcca ctttatcctt ttttaatgct ttgtaaccac cacgagaaaa aatttccgct
     7501 tttgcctcaa cagtagtgag gcgttcatct tgtagctcaa ccggcagatt aaagcgtccg
     7561 tttaagcgat tggcaaattt tctggcacgc tgagttaaag gctgctctgt gccgtccata
     7621 ttcaagggta gcccaacaac cagcatatct ggtttccaat cttgcaagag ttttccgatc
     7681 tcttcccagt ttggaatacc gtcttgggct ttaaatgtag ttaaaccttg agctgtgccg
     7741 gtaatacttt gtccaactgc acagccgatg ctatttgtgc caaaatcaaa ggcaatcacg
     7801 gtttttgttg tggtttctgt cattaagctc tcccagctct tgacggtatg cttgaaagcc
     7861 ccatcatttc ataggcttct gcccaacgct ctaaataacc ggtttcaaac agcgtttttt
     7921 cattcgcatc agacacaatc caataatttt gagcaatttc ttgctcaagc tggctgccac
     7981 gccaagtgga acaacctaaa cacacaataa atttttcagg tgaagccagt gtgccaaagg
     8041 agtctaacac atcaccggag gtagtgagcg taattttatc ggtgatcgga taagtgtgaa
     8101 taaaatcatt ggcggttttg gtgtgaataa taaagccacg atcttgattt accggcccac
     8161 cgcttaacac catttggtct tttttgtaat cacgctggtt cgccatttgg aaatccattc
     8221 gggtaatcag ctctagaacc gacagatcgg taggagtgtt aattactact cccattgcac
     8281 cttgcggatt atgttcgcaa atatagacca ccgtacgctc gaaatagtca tcgtccattt
     8341 ccggcgtggc aattaaaaat ttaccttgta aattctctaa cattaaatat ctccaaaaca
     8401 aacttgtaac gcagtaattg ccgcaagtga ggcggtttct gtgcgtaaca ctcgtttacc
     8461 cagtaatact tcagtaaaac cttcgctctc ggtcatggcg atttcttcgg cagataatcc
     8521 gccctcagag ccaatcagta aacgaacacc agctttcggc acattcggca gttcacgaat
     8581 ggtatatttg gctcttgggt gcaaattcag tttcaacata tcgtcttgtt ctttgcacca
     8641 gtcggtgagt ttcataatcg ggcgaatttc agggatttca tttcgtccgc attgttcaca
     8701 ggcggcaatc gcaattttct gccattgctg taatttttta tcttgccgtt catcattgag
     8761 tttcacgcca caacgttccg accaaagtgg ggtaatagtt tttaccccca gctccaccga
     8821 tttttgaatg gtaaattcca ttctatcgcc tcgggaaatc acttgcccta aatggatcgg
     8881 caggttggat tctcggtcat ctaattcact tgaatcaatt tttgcaataa tttgcttttt
     8941 ttcgaccgct tgtaacgtag cgtggaaaac gtggtttgaa ccatcaaata aaatgatttg
     9001 ctctccttgt ttcattcgta gcactcgccc aacgtggtta gcggcatctt cacttaacac
     9061 gcaactttga agaccagcta acggctgtgg gtggtaaatt cttgggattc tcataattat
     9121 ctagttataa attaaaggca gacgattgtc tgccataggt aagttaaaac gggttaagga
     9181 taaagtcttg aacaatgcac aatagtaata atcgaaattt cattctcttt aacttcacac
     9241 actattctat agccgttatg atatagttct ctcgtattaa taattgcacc ttttacaccc
     9301 attgtaggaa aaatggctaa attattaata ctattttcca attcttctag tatcctaatt
     9361 ccactacttg cataaccggt aaattcacta actgagatgg caatattctc taaatctatt
     9421 aaggcctctt cagtataata aatattatgc aacttttttg gcattttcag cacctctaaa
     9481 agcggcagca ataactcttt ttctgacctc ttcgggtgaa taaatccgac cgtttttaat
     9541 atcttccttt ccttgcttta cttttttagc aagataatca agatattctt ggctaacatt
     9601 atgataagga ttattcatat ttttcctccc tgcgatgagt ctctattgtg ggggctactg
     9661 atcgtttaat caagttaatt tatcttattt ttcgagccac atcacaaaac gagaaaacaa
     9721 caagcggtca tttttccacg ttttttgcaa aaaatcgaag aaagatgacc gcttgccaga
     9781 aaaaattaat tatttgaact ggtgaattgc attggcgatt tcattatcgt tgtaaatagc
     9841 tttcaccaca tcagaatagg cttggtctaa cactttcttg atttcatcat tgcttgcacc
     9901 aaatgcacct tcgtaaccac gggtagcgtt gaagtttttg ctaaagtttc ctttagaacc
     9961 ttgcactgcc acttttacac tcacttgtgc atcggctttg tagcgtaagt ttccttgttc
    10021 tactgtggca aagaatttac gaatattcac taccacattg gcattcgctg caccttgtac
    10081 aatttggaaa ccttttgcat tcaggtcttg ttggacggct tgttggaaca ttgcagcaac
    10141 ttcaggcact gcggttaaac gaatgacctc tccgttttta gtgtagcttg cgatttcacg
    10201 cgaggtgcgt aaatcttgtg tcattacatt caccgcagcc gtttgattgt ttgtgttaaa
    10261 tacagcagtt ggcgttgggg tagagaatgt taatgtgttg gattggcttt ggcagccggc
    10321 tagtaatgcg gcaacaaaag tagtggctaa cactaacgtt tttttagagc gtttcatagt
    10381 aaatccttct atattgaata aatttcgaga aaatttctgc ttagtatagt attatttcgt
    10441 tttgattttc aatgaacgag agcaaaaaat gtcggtagaa cagacaatta ttcaaaaaat
    10501 taagacagaa tttcagccga ttttctttaa tgtcgaaaac gaaagctata tgcacagctc
    10561 aggtagagga gcagaatcgc attttaaatt aactttggtg tcagataaat ttgaagggca
    10621 gagaacagtt gcacgtcatc gtgctgtcta tgcttgctta gctgaggaat tagctaatgg
    10681 cgttcacgca cttgctttgc acctatatac ccaaggtgaa tgggaagcga ctggcggact
    10741 tgtacctaaa tcgccaaatt gcttaggtgt tgggcaataa gtggataaaa atgatttatt
    10801 ttattttgtg aagaaaatac ttgaaagaac cgtgtaaaat tgctatattt acgccatctt
    10861 tttgttgatt cggtgagatg tccgagtggt tgaaggagca cgcctggaaa gcgtgtatat
    10921 gggaaactgt atcgggggtt cgaatccccc tctcaccgcc attcatactg aaagattcct
    10981 tactgttttc atagatattt ccttggttaa gttaaagtag ataatacttt ttcattttta
    11041 ggttatcccc gagcaaacat tgcttgggga tttttttatg cgatcggcga tagattacaa
    11101 agaaaaagga ttcctaagaa tcctttttat ctgcttttaa cgtactttac ctagtttata
    11161 gttattgctg ttttttaccg tgttgccatt tgggtcgcaa agttcaacgt gttgttcaga
    11221 cacttggaag gcaagattgc ctgatgcttt ttccagacga atcacatttg gcttattggg
    11281 ttcccaactg tatgtgcctt tgtctcggta agtgtattct tttttatctt taaaatatac
    11341 cgtatcatat tggtaggttt tatctgcatt taaggttagc tgggcttgga ttttctcaca
    11401 gtcagcacac ggtaattcac ctgcataagt accgctaacg cttttttgtg gtagcaatga
    11461 gcaagcccct agcattgcgg tggtaaaacc aatgagtgcg atttttttca tactattttc
    11521 ctcttcaaaa ttaaattatg taacactcgg atttactcta aattcaagtt gggttatttc
    11581 aggtaaatcg gtttgtatct gttttagtaa catattttgt tgcaagatta aaccttgccg
    11641 tacggttgca ttcttaacgc tcacaataag ctgattatca actaaattca ctactcggta
    11701 aagcccacga taagattctg gccatctttt ctgaattttt tggtttaaat cgttaatgaa
    11761 gtttgctttt tcgacaatac ggcttaagct tgagttctgt aaaatgtcat taatcttttt
    11821 aggtgctgaa tttttcatct gtttctttac atttcgtctt attttaggct tgattttacg
    11881 atacttgacc caataattta ctcattcggc tttactttag cgtataatta gcccaatttt
    11941 ttcgtttatc ttaatttaat gagcttattt ttaaaacact ttcatcgaac gcctttttgg
    12001 tcgcaactct ttttgggaat ggttgcgata cttgctttac ctgaaattca ggttgcgtac
    12061 gccaatgaaa gtgaacagga aaccgttctt aatcaatctt ttcttcaata tgctcaatcc
    12121 gctaatgata ttgagcaaca atcgcttttt attgcagagt taatccataa aattgtggaa
    12181 attaaaccac aagcggtgca tttttgcgaa ttttttgcaa actcctaccg cttggataga
    12241 caaaatgctt accccattcg agccgggcca caaacctaaa tataatttaa ttttaccatt
    12301 aattgattaa cccattttta tatttaggaa taacacaatg atttcaaaat tactgacttc
    12361 tatttttggc tcaagcaacg atcgcacttt aaaacgttta agaaagcgag tggcacaaat
    12421 taataagctt gaaccaacct tcgagcaatt atctgatgag gcgttaaagg ataaaacagc
    12481 ggagtttaag caacgccttg cagatggtgc atcgctagat agcctattac ctgaagcttt
    12541 tgcgacagtg agagaagcaa gcaaacgagt attaggtatg cgaccatttg atgtacagct
    12601 gatcggcggt atggtgctaa cggggcgtaa tattgcagaa atgcgtactg gggaaggtaa
    12661 aaccctaact gcaactttat cttgttattt gaatgcttta accggtaaag gtgttcatgt
    12721 tgttacggtg aatgattacc tcgcccgccg tgatgcggaa accaaccgtc cgttatttga
    12781 gtttttaggc ttaaccgttg gagtgaatat tccggggtta ccttctgatg caaaacgtgc
    12841 tgcataccaa gcagatatta cctattcgac taacagcgaa ttaggttttg attacttgcg
    12901 tgataactta gctcacgata aaaacgaacg tttccaacgt gagttacact acgcattagt
    12961 tgatgaagta gactctattt taattgatga agctcgtacg ccgttaatta tttcagggcc
    13021 tgcggaagat gcgacccaaa tttatcaagc ggtagatcaa atcattccgc atttgattgc
    13081 tcaagataaa gaagacagtg aagagtacac cggtgagggc gattttacct tagatctgaa
    13141 aaacaaacag gctcatttaa ccgagcgtgg tatggtgaaa gtagaggatt tcttaactca
    13201 aatggggtta atggaagagg gcgaaagttt atatcaccct gctcgtattt cgctgttaca
    13261 ccatgtgagt gcagcattgc gtgcccataa attatttgaa cgcaacgtag attacattgt
    13321 aaaagacggt gaaattgtga ttattgatga gcacaccggt cgtacaatgg ctggacgtcg
    13381 ttggtctgac ggcttacacc aagcgattga agctaaagag cgtgtggcta ttcagggcga
    13441 aaaccaaact gttgcctcaa ttacttacca aaactatttc cgtttgtacg aaaaattagc
    13501 cggtatgact ggtacggcag acaccgaagc ctttgaattc cagcaaattt atggcttaaa
    13561 tacggtggtg attccaacca acagaccggt gatccgtgat gaccgtacag acttaatgtt
    13621 taaaagcgaa gcggagaaat ttgcggcgat tattactgaa attagagact gtatcgagcg
    13681 tgaacagcct gttttggttg gcacagcatc ggtggaaaaa tcagagttat tatcggcaga
    13741 attaaccaaa gccggtattg ctcataacgt attaaatgcg aaattccacg ctcaagaggc
    13801 tgaaattgta gcggaagcgg gagctccggg ggcggttact atcgcaacca atatggctgg
    13861 tcgtggtacg gatattgtgc ttggcggtaa ctggaaaatg gaagtagcca agcttgagaa
    13921 cccgacacaa gaacaaattg atgcgattaa agctgcgtgg caagagcgtt atgacatcgt
    13981 aatgcaagcc ggtggtttac atattattgg tacagaacgc cacgaatccc gccgtatcga
    14041 caaccagttg cgaggtcgtt ctggtcgtca aggtgacccg gggtcatcac gtttctatct
    14101 ctctttagat gatgccctaa tgagaattta cctcaatgaa ggtaaattaa atatgatgcg
    14161 taaagccttt accgaagaag gtgaagcgat ggagtctaaa ctgttaacca aagtgattgc
    14221 ctctgcacag gcgaaagtag aagcgcataa ctttgatggg cgtaagcatt tattacaata
    14281 tgatgatgtt gccaatgaac agcgtaaagt aatttacgaa caacgcaatc atctattaga
    14341 aacagataat attgcctcaa tgatcgaaag cattcgccaa gatgtatttg aaagcacgat
    14401 taaccaatat attccgcctc aatctatcga ggaaatgtgg aatgtgccgg gcttagaaga
    14461 gcgtttacgc cgtgaatttg ggttggattt accgatttct cactggcttg aaactgaaaa
    14521 agacttgcac gaagagactt tgcgtgaacg tatcttaaat attgcgattg cagagtacaa
    14581 tgcgaaggaa gaaatagtag gcgcagaggt aatgcgtaat tttgaaaaag gtgtaatgct
    14641 acaaaactta gatgagcttt ggaaagagca tctagcctca atggattatt tacgcaaagg
    14701 tattcacttg cgtggttatg cacaaaaaga tccgaagcaa gagtataaaa aggaatcttt
    14761 tgcgatgttt accaatatgc ttgatacctt aaaattgaat gtaattagca ttttaagccg
    14821 tattcaagtg cgtagcaaag aagaggtgga agaagcgcaa cgccagcaag ctgaaatgga
    14881 cgctgcaagt acgccagtta cacaagctac cgattctgct gctggtagta gagaagaaaa
    14941 tagcgcagag agcttaagtg aagaagagtt ggcaaactta aatattggtc gaaatgaccc
    15001 ttgcccttgt ggttcgggca aaaaatataa gcattgccat ggtaacaaag cgagatattc
    15061 gtaattaaca agcggtcata ttcagtcagg aatttgcaaa ttttctgctt aatatgaccg
    15121 cttgaattta tagaaagagt gaaggaaact atgtcaaaac cgattattca agtctcagca
    15181 gggattattc gtaacgaatt tgggcaaatt tacttaaccc aacgtttaga agggcaagat
    15241 tttgctcagt ctcttgaatt tcccgggggg aaagtcgatc ccaatgaaac ccctgaagaa
    15301 gcactaaaac gagaattaga agaagaaatt ggtattcaag tgttaagcgc ttttccgtat
    15361 gaaaattttt gctttgaata tccaacaaaa atcattgaat ttttctttta tttggtggaa
    15421 gagtgggtaa atgaacctta cggaagagaa gggcaagaag gtttttggat cgcacaatcg
    15481 gatttagatg agggagcttt cccaccggca aatgcccaat taattaaacg cttaaaagaa
    15541 cacgcataat ttgtattgag aactgtatct aaagtaattt agctgcggtt ttttattgga
    15601 tacgctattg agacatttat aaagctgatg taggggcgaa ttactattcg ccctttcttt
    15661 tttgcagagt tttagtaaaa atgaccgctt ggtataccta ataaataatg attgtatcgg
    15721 atttagcaca acttatatta ttgaggagcg tcattaaaaa atggtaat
//
LOCUS       DANJWC010000044        15461 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.044, whole genome shotgun sequence.
ACCESSION   DANJWC010000044 DANJWC010000000
VERSION     DANJWC010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15461
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.044"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..167)
                     /locus_tag="PWX02_001016"
     CDS             complement(<1..167)
                     /locus_tag="PWX02_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL1729042.1"
                     /translation="MKAQQLKNAILQLAIQGKLVPQDPNDEPASELLCKIQAEKDRLI
                     AEGKIKKNKKTA"
     gene            complement(373..2034)
                     /locus_tag="PWX02_001017"
     CDS             complement(373..2034)
                     /locus_tag="PWX02_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HDL1729043.1"
                     /translation="MLTWEHIEKMIAHQQFIREKAEIEYKEAKNSVPKDLWHTYSAFA
                     NSNGGFILLGIKENEQGHFPELLISGVNDPDKVIDELFSQSRSEKVSKSLLKDNDVKI
                     FQVENKSVIAIYVKAADILDRPIHLNGDYRLSYVRLNSGDHKLTSSELRSFISSYSQK
                     DADGRIVPYASIDDISLSTLDKYRNLLKAHNSTSKLLTLSDEKLLQEIGAYKKDLAQN
                     SEGITNAGLLMFGKHYAITSIFPHFFFEYYEEAGDVKRYTLRITDFDLEEGNLFEFYL
                     KIVPLIQELGKDKHFKLDNLTRTEENEITDGLREALINAITHADYFNNVRHLKVIKTK
                     DNKLIFENAGVMLVDIPTAIAGQRSECRNSIIHNLFHRLGLCERQGQGVRDIYSYWQS
                     RNFNSPLLESEIDYTKLTLTFQSGMIADFEEKFTACFGKNFSELQPIQKDCLLFIAFN
                     QGKAMFAELAQKLPQYPRRNITLALARLKEKGLLTSSGSPRKQLYTLVNLEWIITEKS
                     QDDYFNPPFDQLDKASESKGAPPKKSKVKQVKQLPQNKANKLPPK"
     gene            complement(2047..3516)
                     /locus_tag="PWX02_001018"
     CDS             complement(2047..3516)
                     /locus_tag="PWX02_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDL1729044.1"
                     /translation="MSLNNIVKRLQDVMRNDAGINGDAQRIEQIVWILFLKIYDAKEQ
                     EWELENDEYHSILPNFLRWQNWAEDKKDGKAMTGDELLSFVNNELFPTLKNLPISADT
                     PMNQRIIRAAFEDNNNYMKNGVLLRQVINIIDEIDFAHYQERHAFGDIYENILKSLQS
                     AGNAGEFYTPRAVTDFMVQAIKPKLGERIADFACGTGGFLTSALKVLESQIQTLSDRT
                     LFNNSVYGIEKKALPHLLCITNLLLHDIDNPNVHHDNALEKSVKDYTENDKFDVILMN
                     PPYGGSEIEQIKKNFPTALQSSETADLFMSVIMYRLKQNGRAAVVLPDGFLFGTDNAK
                     VAIKKKLLEEFNLHTIIRLPHSVFAPYTSITTNILFFDKTEPTRETWVYRLDMPQGYK
                     NFSKTKPMKLEHFNEVVEWWGNRQVIEVEGFDKAKSFTLKEISDRQYNLDLCGFPHEE
                     EEILPPDELIADYQRKRTAYNADIDKILAEITDILGIKL"
     gene            complement(3564..5915)
                     /locus_tag="PWX02_001019"
     CDS             complement(3564..5915)
                     /locus_tag="PWX02_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL1729045.1"
                     /translation="MYTLQELKQMNEQEIRSRLISPAIRNAGWSDRQIGEEYTFKTNK
                     RFTDGQVVVDPKTTQTKRIEAKRVDYLLYTSANQKIAVVEAKDNHHSSRHGLQQAMTY
                     ARLLDVPFAYSSNGDEFVEHDFITGVQRTLPMSAFPTPDELHQRWAKQYTTEELKIIQ
                     QPYYTSSTDTFKEPRYYQEIAINRTVEAVAKGQQRILLVMATGTGKTYTAFQIIHRLK
                     TSGAKKKILFLADRNVLVDQPMMQDFKPFKDKMVKVSNKKLDSAYEVYLALYQQLDGE
                     TDNELFKQFQPSFFDLIVIDECHRGSAKEDSDWRAILKYFKDATHIGMTATPKETDDA
                     SNITYFGEPIYTYSLKQGIEDGFLAPYRVIRVDLDCDLKGWRPTKGLKDHQGNEIEDR
                     LYTVKDFDYNIVLNDRTRLVAERICEFLRKTDRLQKTIVFCANINHAERMVAELSRLN
                     ADMLKHNSKYVCQITGDQSGKGVDLEEFCDVANEGKDYPVIAVTSKLMTTGVDSKTCK
                     LVVLDANIQSMTEFKQIIGRGTRLRPDFGKEFFTILDFRGVTRLFADPAFDGEPVVIL
                     DVPENKPLVEQPLPKPSDKDIIKDPELKPYKIPVVVDFGTLKLRILDEKVQYLNEQGE
                     LVSEDIDLFSKREMQKEFGSYEQFQQQWTTSGEIFSKFYTDPKWLAALRQTRQFSHDV
                     EDFDVLSHISFGKKPLTKTERAEKVKQSGYVEQYSPENQQVLGLLLNEYVKSGNQDLT
                     KTEILKTPQFSRFGGLVSIIKNFGGKELYQKAVKGLEKMIYSE"
     gene            complement(5987..6880)
                     /gene="psd"
                     /locus_tag="PWX02_001020"
     CDS             complement(5987..6880)
                     /gene="psd"
                     /locus_tag="PWX02_001020"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251643.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDL1729046.1"
                     /translation="MQLKKYPVPTYFQRVKIALHYLLPQLAITRAAGWLAEQKWGVVT
                     HLIIKLFAKQYKVNLSEAKKTEPSDYATFNEFFIRELKENARPINQDPNTLCLPADGK
                     VSESGEISDNRLLQAKGHHFTLETLLANDEEMAAKFKDGTFITTYLSPTDYHRVHMPC
                     DGTLRKMIYVPGELFSVNPFLAEHVPNLFARNERVICEFDTAFGPMLQILVGATVTAS
                     MSTVWAGVINPPRADEVVVWNYETEGEKAIKLQKGEEMGAFRLGSTVINLFPKGKVHL
                     NPALIAGMQTRMGEELGRIIN"
     gene            complement(7045..7383)
                     /locus_tag="PWX02_001021"
     CDS             complement(7045..7383)
                     /locus_tag="PWX02_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HDL1729047.1"
                     /translation="MKKIKQLGLTFLFGLNAMAFAQTPPTTMPVEQNAVTQPVQQTQI
                     TNANAVNINTASAAELQDKLVGIGQKKAQAIVDYRTKNGNFKNIEQLAEVSGIGQATV
                     EKNRANIILE"
     gene            7676..8995
                     /locus_tag="PWX02_001022"
     CDS             7676..8995
                     /locus_tag="PWX02_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="opacity-associated protein A"
                     /protein_id="HDL1729048.1"
                     /translation="MTQNNFRKEPVFGESSVTSQDAEATKADTAQAPYVSLRSTKAPG
                     HTFTPIMKRPDAKVEDHTADVFSAKQAKEFQFTPSSEATEAVEPDNTIHSVEESVSTA
                     SFNTVEAQENVEKEVVKPAPESFTTERIIPNAATAAAAATAAAAATKTKEIKAKMPPK
                     TRRLLLVALLALALLVLFFLLKPSTPETVEELQSQQGGSLPIEFRPVDEAEAKHAEEQ
                     ARAEQEAAEAAKAQEQAQAAQQESVTVAPAQTEQTMAKPSQDSQSQTQSQAVVEQPVA
                     VKPVENKPVVAQPAIKPKTENSVVFQPETTVKQQPKAPAKTEVKTERATLAAKETAKT
                     AAKPVEKAPTKTEAVKAAPAAESANAVATKTMTVPKDVSLMQVFRDNNLNISDVNAMS
                     KVNNVVSNLKVGERVTVRLDKNNRVVEMSIGSGGKFTRQANGSYTFK"
     gene            9109..9471
                     /locus_tag="PWX02_001023"
     CDS             9109..9471
                     /locus_tag="PWX02_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HDL1729049.1"
                     /translation="MLVACSTVVTPVEQIHQVQKEKEPVKKVTKLIAQAPKVSLYRCE
                     NDKKVEVERQGRTKKALKKEIVTVSYQGTTHQLSPSVTRDGKKYTNIRWTWHETRSGK
                     AFLYNNTKKTLAANCIKQ"
     gene            9582..10619
                     /gene="waaF"
                     /locus_tag="PWX02_001024"
     CDS             9582..10619
                     /gene="waaF"
                     /locus_tag="PWX02_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HDL1729050.1"
                     /translation="MNILVIGPSWVGDMMMSHALYQQLKIQYPNCQIDVMAPDWCRPL
                     LARMPEVRNAISMPIGHGSFRLCERYRLGKSLRNQYDMAIVLPNSLKSAFIPLFAKIA
                     RRRGWKGESRYFFLNDLRSNKNDYPMMVQRYVALAFEKATVPTAQQLPIAYPYLTTDS
                     EQIEQTKAKFEKQLAYAENRPAIGFCPGAEFGPAKRWPHYHYATLAKMLIEQGYSVRL
                     FGSKKDEAAGEQIRLALPENLQRYCLNLAGQTDLNQAVDLIADCNAVVSNDSGLMHIA
                     AALNKPLVALYGPTSPQYTPPLSQNAVIIRLIEGGLIKIRKGEGAEGYHQSLIDIQPE
                     MVMDKLAELLI"
     gene            10687..11646
                     /gene="rfaC"
                     /locus_tag="PWX02_001025"
     CDS             10687..11646
                     /gene="rfaC"
                     /locus_tag="PWX02_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HDL1729051.1"
                     /translation="MKILIVKTSSMGDVLHTLPAITDMQNAIPDLKVDWVVEENFTEI
                     PAWHSAVNKVIPVAIRRWRKNLCQRQTWVEWQQFLKQLKSEQYDAVIDAQGLIKSAVL
                     VTRQAKGKKFGYDKNSAREGLSSLFYDETFNIPYQQHAVERIRKLCAKSLGYALPTER
                     GDYCIAQHFAKNPQKTTAYIMLIHATTRADKHWQEAYWAELIQALDKHNLAVRLPWGN
                     QAEKERAERLASVANNAKVLPKMSLTDIATQLAGAAAVVSVDTGLSHLAAALDKPNVI
                     LYGATDPKLIGAYGQNQFYLKADKMENITSSQVLEKLLPLINK"
     gene            11767..12141
                     /gene="rpsL"
                     /locus_tag="PWX02_001026"
     CDS             11767..12141
                     /gene="rpsL"
                     /locus_tag="PWX02_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDL1729052.1"
                     /translation="MATINQLVRKPRVKKVVKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRIRLTNGFEVTSYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGAL
                     DCAGVKDRKQGRSKYGVKRPKA"
     gene            12272..12742
                     /gene="rpsG"
                     /locus_tag="PWX02_001027"
     CDS             12272..12742
                     /gene="rpsG"
                     /locus_tag="PWX02_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDL1729053.1"
                     /translation="MPRRRSIEPRKILPDPKFGSELLAKFINVLMVDGKKSTAEKIVY
                     GALETLAQRSGKEALEAFEIALENVRPTVEVKSRRVGGSTYQVPVEVRPTRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAADNKGSAVKKREDVHRMAEANKAFAHFRW"
     gene            12844..14946
                     /gene="fusA"
                     /locus_tag="PWX02_001028"
     CDS             12844..14946
                     /gene="fusA"
                     /locus_tag="PWX02_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL1729054.1"
                     /translation="MARTTPISLYRNIGISAHIDAGKTTTTERILFYTGVSHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMSQQYPQHRINVIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRTGANFLRVVEQIKT
                     RLGGNSVALQLPIGAEDSFKGVVDLIKMKAINWNEADQGMTFTYEDIPADMLEACEEH
                     RAMLVEAAAEASEELMEKFFSGEELTEDEIKAALRQRVLAGEVIPVCCGSAFKNKGVQ
                     AMLDAVIDYLPAPTDIPAIKGINEDETEGERHASDDEPFASLAFKIATDPFVGNLTFF
                     RVYSGVINSGDTVYNSVKQKRERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVGTG
                     DTLCAIDAPIILERMEFPEPVISVAVEPKTKADQEKMGLALGRLAQEDPSFRVHTDEE
                     SGETIISGMGELHLDIIVDRMKREFKVEANIGKPQVSYRETIRTRVNDVEGKHAKQSG
                     GRGQYGHVVIDLYPLDAEGPGYEFVNEIKGGVIPGEYIPAVDKGIQEQLKSGPLAGYP
                     VVDLGVRLHFGSYHDVDSSELAFKLAASLAFKAAFAKASPVLLEPIMKVEVETPPDYV
                     GDVIGDLSRRRAMVNGQEANEFVVKINAEVPLSEMFGYATDLRSQTQGRASYSMEPLK
                     YAEAPKNVADAIIEARRK"
     gene            15006..>15461
                     /locus_tag="PWX02_001029"
     CDS             15006..>15461
                     /locus_tag="PWX02_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDL1729055.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKHFGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTATRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEM"
BASE COUNT         4584 a         3019 c         3264 g         4594 t
ORIGIN      
        1 gcagtttttt tgtttttttt gatctttcct tctgcaatca agcggtcttt ttctgcttgg
       61 attttgcaaa ggagttcgct tgccggttca tcatttggat cttgtggaac gagcttgcct
      121 tggattgcta gttgtaggat tgcgtttttt agctgttggg ctttcatttt ttattctcaa
      181 taatcggttg ttatttctta aagtatcttt gattacggtg ttttgggttt tctgggaatt
      241 gcatttcgat cacaccaagc gttagtgcag gttttaaata ctgcatacgg aaatgcttat
      301 catcttttaa cctgagtttt ttcataattt ctttgctctt catcggctca agcaacactg
      361 aaagtaagcg atttacttcg ggggtaattt gtttgctttg ttctggggta attgtttgac
      421 ttgcttcact ttgcttttct tcgggggggc gcccttactc tcactagctt tatcaagttg
      481 gtcgaaaggt ggattaaaat aatcatcttg tgatttctct gtgattatcc attctaaatt
      541 cactagggta tagagttgtt ttctcggact ccccgaactg gttagtaagc ctttttcttt
      601 cagtcgtgct agtgccaaag taatatttct gcgtggatat tggggcagtt tttgtgctaa
      661 ttctgcaaac attgctttac cttgattgaa ggcgataaag agtaagcaat ctttttgaat
      721 aggctgtaac tcagagaaat ttttgccaaa gcaagcggta aatttttcct caaaatctgc
      781 aatcatacct gattgaaaag ttaaggtgag tttagtgtaa tcaatttcac tttctaaaag
      841 cggtgaattg aaatttcggc tttgccaata tgaataaata tcccgaacgc cttgtccttg
      901 tcgctcacat agccctaaac ggtggaataa gttatgaata atagaattac ggcattccga
      961 tctttgccct gcaatagccg tgggaatatc aaccaacatt actcctgcat tttcaaaaat
     1021 cagtttgtta tctttggttt taatgacttt tagatggcga acattgttaa agtaatctgc
     1081 gtgagtaatt gcattgatga gagcttcccg tagcccgtca gtaatttcat tttcctcagt
     1141 acgggttaaa ttatctaatt tgaaatgttt atctttacca agctcttgaa taagtggcac
     1201 aattttcaag taaaattcaa agagattgcc ttcttcaaga tcaaaatcgg taatccgtaa
     1261 tgtatagcgt tttacatcac cagcttcttc gtaatattca aaaaagaagt ggggaaatat
     1321 gcttgtaatg gcatagtgct taccaaacat caataagcct gcattggtaa tgccctcgct
     1381 attctgtgct aaatcttttt tataagcccc aatttcttgc agcaatttct catcggataa
     1441 cgtaagtaat tttgatgtgc tgttatgggc tttcagcaga tttctgtatt tatccaatgt
     1501 gcttaaactg atatcatcaa tagaagcata aggcacaatt ctcccatcag catctttttg
     1561 gctatagctt gaaatgaaag aacgcagttc agaggaggtg agtttatgat cgcctgaatt
     1621 taaacgaaca taagaaagtc gataatcacc atttaaatgg ataggacggt ctaaaatatc
     1681 agctgctttc acataaattg caatcacgct tttattttca acttgaaaga ttttcacatc
     1741 attatccttt aataaggatt tactgacttt ttcactacga gattgggaaa ataattcatc
     1801 aatgacttta tcaggatcat taacaccaga aattagcagt tcaggaaaat gtccttgttc
     1861 attttccttt attcctaata aaataaagcc accattgctg tttgcaaaag ccgaataagt
     1921 atgccaaaga tcttttggca cactattttt tgcctcttta tattcaattt ctgccttttc
     1981 tcggataaat tgctgatgtg caatcatttt ttcgatatgt tcccaagtca gcattttatg
     2041 ctcctattac agtttaatgc ctaaaatatc ggtaatttct gctagaatct tatcaatatc
     2101 agcattgtaa gcggtgcgtt ttcgctgata atctgcaatt agttcatcag gcggaaggat
     2161 ttcctcttct tcgtgaggaa agccacataa atcgaggttg tattgacggt cggaaatttc
     2221 ctttaatgta aaacttttcg ctttgtcaaa cccttcgact tcaatcactt ggcgatttcc
     2281 ccaccattca acaacttcgt tgaaatgttc caatttcatc ggtttagttt tggagaaatt
     2341 tttataaccc tgtggcatat caaggcgata aacccaagtt tcacgggttg gctcggtttt
     2401 atcaaaaaac agaatattag tcgtaatcga ggtataaggg gcaaagacac tatgcggtaa
     2461 gcggataatc gtatgcagat taaactcttc taatagcttt ttcttaatcg caactttggc
     2521 attatctgtg ccgaacaaaa atccgtcagg cagcaccacc gcagcccgtc cattctgttt
     2581 taggcggtac ataattaccg acataaacag atcagcggtt tcactgctct gtaaagccgt
     2641 agggaaattc tttttaattt gctcaatttc tgaacctccg taaggtggat tcatcaaaat
     2701 cacatcaaat ttatcatttt cggtgtaatc tttgaccgat ttttctagag cattatcgtg
     2761 atgtacattc ggattatcaa tatcgtgcaa caataaattg gtaatacaaa gcaggtgagg
     2821 aagggctttt ttctcaatgc catacacaga attattgaat aaagtgcggt cagaaagggt
     2881 ttgaatttga ctttccaata cctttaacgc cgaagttaaa aaaccacccg tcccacaggc
     2941 aaaatcggca atgcgttcgc caagtttcgg tttgattgct tgtaccataa aatcggttac
     3001 agcgcgaggg gtataaaatt cccctgcatt acctgcactt tgtaaacttt tgaggatatt
     3061 ttcataaata tcgccaaagg cgtgtcgttc ttggtaatgg gcaaaatcaa tttcatcaat
     3121 gatattgata acctgacgta ataacacccc atttttcata tagttgttat tatcttcaaa
     3181 agcagcacgg ataatccgtt ggttcatcgg cgtatcggct gaaataggca gattttttaa
     3241 agtcgggaaa agttcgttat tgacgaaaga gagtaattca tcaccagtca tcgctttgcc
     3301 gtctttttta tcttccgccc aattttgcca gcgtaaaaaa ttcggcaaaa ttgagtgata
     3361 ttcatcgttt tccagttccc actcttgttc tttagcatcg taaattttca agaataagat
     3421 ccagacgatc tgttcaatgc gttgggcatc accgttaatc cctgcatcgt tacgcattac
     3481 atcttgcagg cgttttacta tgttgttgag gctcattgtg atccttgttt atctgtaaaa
     3541 ttttgttgta aaataaccgc ttgttattcc gaataaatca tcttttctaa gccttttacc
     3601 gctttctggt agagttcttt accgccgaaa tttttaataa tagacactag acctccaaaa
     3661 cggctgaatt gcggtgtctt taaaatttcg gttttagtca gatcttgatt gccggatttg
     3721 acatattcat taagaagcaa ccctaaaact tgctggtttt cgggcgaata ctgctccaca
     3781 tagccacttt gtttcacttt ttctgctcgt tcggttttag tcagtggttt tttgccaaag
     3841 gagatgtggc ttaatacatc aaagtcttca acatcgtggc tgaattggcg tgtttggcga
     3901 agggctgcca gccattttgg atcggtgtaa aatttcgaga aaatttcacc gcttgttgtc
     3961 cattgctgtt ggaactgttc gtaactgccg aactcttttt gcatttcccg ttttgagaac
     4021 agatcaatat cttcagaaac cagctcgccc tgctcattca gatattggac tttctcgtct
     4081 aaaatgcgta atttgagtgt gccgaaatcg acaactaccg ggattttata tggctttagc
     4141 tcggggtctt taatgatgtc cttatcactg ggtttaggaa gcggttgttc gacaagtggc
     4201 ttattttccg gtacatctaa aatcaccacc ggctcaccat caaatgccgg atcagcaaac
     4261 aggcgagtaa cgcctcgaaa atctaaaatg gtgaaaaatt ctttgccgaa atcagggcga
     4321 agacgagtgc ctcgtccgat aatttgttta aattccgtca ttgattgaat gtttgcatca
     4381 agtacaacca gcttacaggt tttgctatct acacctgttg tcatcaattt agaggttacg
     4441 gcaatcacag ggtaatcttt cccttcattt gctacatcac aaaattcttc taaatcaacg
     4501 cctttgcctg attggtcgcc tgtaatttgg cagacatatt tggaattgtg tttcaacata
     4561 tcggcattga ggcgtgaaag ctctgccacc attcgctctg catggttgat gttggcacag
     4621 aagactattg ttttctgcaa gcggtcggtt ttgcgtaaaa attcgcaaat tctctccgca
     4681 accaagcggg tgcgatcatt tagcacaatg ttgtaatcga aatctttgac tgtatagagg
     4741 cgatcttcaa tctcattacc ttgatgatct tttaaacctt tggttggtcg ccagcctttt
     4801 aaatcacagt ccagatcaac acgaatcact cgataagggg caagaaagcc gtcttcaata
     4861 ccttgtttga ggctataggt ataaatcggc tcgccaaaat aggtaatgtt gctggcatca
     4921 tcggtttctt tgggggttgc agtcataccg atatgggtgg cgtccttgaa atacttcaaa
     4981 atagcacgcc agtcagaatc ttcttttgct gaaccacgat gacattcatc gatcacaata
     5041 agatcaaaga aagagggttg gaactgctta aacagttcgt tatcggtttc accgtcaagc
     5101 tgttgataaa gtgcaagata gacttcataa gcactgtcga gctttttatt gctgactttc
     5161 accattttgt ctttaaacgg tttgaagtct tgcatcattg gctgatcgac tagtacattg
     5221 cgatcggcaa gaaataaaat tttctttttc gcaccactgg tttttaaacg atggataatt
     5281 tggaaagccg tataggtttt acccgtgcct gttgccatta ccaataaaat cctctgctga
     5341 ccttttgcta cagcttcaac agtacggtta atcgcaattt cttggtagta acgaggctct
     5401 ttaaaggtgt cagtgctact ggtgtaataa ggctgttgaa taattttcag ctcttcggtg
     5461 gtatattgtt tcgcccaacg ttggtggagt tcgtcgggag taggaaatgc tgacatcgga
     5521 agggttcgtt gtacgccagt gataaaatcg tgttccacaa actcatcacc atttgagcta
     5581 tatgcaaaag ggacatcaag caatcgagcg tatgtcattg cttgctgtaa gccgtgtcgg
     5641 gaagagtgat ggttatcttt ggcctcgacc acggcaattt tttgatttgc ggaagtatag
     5701 agcagataat caacccgttt tgcttcgatg cgtttagttt gcgtcgtttt tggatcaaca
     5761 actacttgcc catctgtaaa tcgtttattg gttttaaatg tgtactcttc gccaatttgt
     5821 ctatccgacc atcctgcatt tctaattgca ggagagatta atcgagagcg aatttcttgc
     5881 tcgttcatct gctttaattc ttgaagtgtg tacatatttt tccttccgat aagagtttct
     5941 tatcggaaga ttatagcaaa cagtttaggt aaaattaact caggatttaa ttgataattc
     6001 tacccaactc ttcccccatt cgggtttgca ttcctgcaat aagtgcaggg ttgagatgta
     6061 ctttaccttt cgggaagagg ttaattacgg ttgagcctaa acggaaagct cccatttctt
     6121 cgcctttttg gagtttgatg gctttttcgc cttcggtttc gtaattccaa actaccactt
     6181 catcagctcg aggcggatta attacgccag cccaaaccgt actcatactt gcggtaaccg
     6241 ttgcaccgac caaaatctgt agcattggtc cgaatgcggt gtcaaattca caaatcacac
     6301 gttcattgcg agcgaataag tttggaacgt gttcggctaa aaacggattg acggagaaca
     6361 attcgcccgg cacataaatc attttacgca atgttccatc gcacggcata tgtacacgat
     6421 ggtaatcagt tggcgataag taggtggtaa taaatgtgcc gtctttaaat tttgccgcca
     6481 tttcttcatc atttgccaat aaggtttcta gcgtaaaatg atgccctttt gcttgtaata
     6541 aacggttatc cgaaatttcg cctgattcgc tgactttacc gtctgccggt aagcaaagtg
     6601 tatttggatc ttgattaatc gggcgtgcgt tttcttttaa ttcacggata aaaaattcat
     6661 taaaagtagc ataatctgac ggctcggttt ttttagcttc agataaattg actttatact
     6721 gttttgcaaa cagtttgatg ataaggtgag taactacgcc ccatttttgc tctgctaacc
     6781 aacctgccgc acgggtaatt gccagttgtg gcagtaaata gtgcagagcg attttcacac
     6841 gttggaaata ggttggaact ggatattttt ttaattgcat tgcgttttcc ttttatttaa
     6901 aaacgagaat ttgcgtttaa ttgggggcga agtatagcaa atacaatgca aaatagataa
     6961 aaaatcttta aggtgttgaa gtggctacaa agaaaaaaac agataggggc tttgtaggaa
     7021 ttccacttca acaatttggt tgtcttattc taaaataata ttggctcgat ttttttctac
     7081 cgttgcttgc ccaattccgg agacttcagc aagttgctca atgtttttaa aattgccgtt
     7141 tttagtacgg taatcgacaa tcgcttgagc ttttttctgc ccaattccca ctagcttgtc
     7201 ttgcaattca gcagcactag cagtattgat atttaccgca ttggcgttgg tgatttgtgt
     7261 ttgctgtacg ggttgagtga ctgcattttg ttcaacaggc attgtagttg gcggtgtttg
     7321 ggcaaaagcc attgcattta agccaaacaa gaatgttaga ccaagttgtt tgattttctt
     7381 cataggattt ccttattagt tgttgaaagg tcgaaaggaa gattaagagg gaataaaata
     7441 gaactcaacc ggcaaaaata aatttttcga cacagatcga aaaaatagaa aatggctaaa
     7501 cttctagact tgcatttggt ctgaaattca atgaaagtaa atagatttgc aaaaaattag
     7561 gcaaaaatga ccgctagttg cgtaaattta gttaaataaa ttcaaatagc ggctgaattg
     7621 ctttagaata tcgccatttt tatactcatt aacaacgcta ttatttggaa ttaatatgac
     7681 acaaaataat tttcgcaaag aaccggtttt tggtgaatct tcggttacct ctcaagacgc
     7741 tgaagcaacc aaagcagata cagctcaagc cccttatgtt tcgttgcgtt caaccaaagc
     7801 accggggcat acgtttaccc caattatgaa aagacctgat gctaaagtag aagatcatac
     7861 ggctgatgtc ttttcggcta agcaggcaaa agaattccaa tttactccaa gttcagaagc
     7921 tacagaagca gtagagccgg ataataccat tcattcggtt gaagaaagtg tatcaaccgc
     7981 atcttttaac acggttgaag cacaagaaaa tgtggaaaaa gaagtggtta aacctgcacc
     8041 cgagagtttc actactgagc gtattattcc aaatgcggca acagctgccg cagcagccac
     8101 tgcggcagct gcagccacta aaaccaaaga gattaaagct aaaatgccac ctaaaacccg
     8161 ccgtttatta ttagtggcat tgttggcgtt agcattattg gtactgtttt tcttgttaaa
     8221 accaagtacg ccggaaactg tcgaagagct acaatctcag caaggtggta gcttgccgat
     8281 tgaattccga ccggttgatg aagctgaagc gaaacatgcg gaagaacaag ctcgtgcaga
     8341 acaagaagcc gctgaagctg caaaagctca agaacaagct caagccgctc aacaagagtc
     8401 tgtaacagtt gctccagccc aaaccgagca aacaatggct aaaccgtcac aagatagcca
     8461 gtcgcaaaca caatcacaag cagtagttga gcaacctgtt gcggtgaaac cggtggaaaa
     8521 taaaccggtt gttgcacaac cggcgattaa gcctaaaacg gaaaatagcg tggtgttcca
     8581 acctgaaaca acggtaaaac aacagcctaa agcccctgct aaaactgaag tgaaaaccga
     8641 acgtgctaca ctggcagcaa aagaaacggc aaaaacagca gctaaaccgg ttgagaaagc
     8701 tccgacaaaa actgaagcgg taaaagcagc accggctgcc gagtcggcaa atgcggtagc
     8761 aaccaaaaca atgactgtgc caaaagatgt atcactaatg caagtgttcc gtgataacaa
     8821 tttaaatatc tcagacgtta atgcgatgag caaagtcaat aacgtggtga gtaatttaaa
     8881 agtaggcgag cgagtaaccg ttcgtttaga taaaaataac cgtgtggttg aaatgagcat
     8941 tggctcaggc ggcaaattta cccgtcaagc aaacggtagc tatactttta aataaaatga
     9001 agaaaaagag caacgctaca aacgttgctc ttttatttaa aaaggagata aaggatgaat
     9061 ttattttgcc cctcttacaa acttatctac ttaacgctaa gctcagcgat gttagttgct
     9121 tgtagtacag tggttacccc tgttgagcaa attcatcaag tacaaaagga aaaagagcca
     9181 gtaaagaaag tgactaaact gatagcgcaa gcacctaaag tgagtttata tcgttgtgag
     9241 aatgataaga aggttgaagt tgaacgccag ggacgaacaa aaaaagcgtt aaaaaaggaa
     9301 atagtcacag tgagttatca agggacaacg catcagcttt caccttctgt aacgagagat
     9361 ggtaaaaaat acactaatat ccgttggact tggcatgaaa cccgtagtgg taaagcattt
     9421 ttgtataaca acactaaaaa aacgttagcg gcaaattgca taaagcagta acaagcggac
     9481 acaaaaagta tcgctttttg aacgttggct ttgccaacgg cagcgaagct gtgaacaatc
     9541 cttacaagga ttgtgaataa atttttaaga gaattttgca aatgaatatt ttagttatag
     9601 gaccgtcttg ggtgggtgat atgatgatgt cgcacgcact ttatcaacag cttaaaatac
     9661 agtatccgaa ttgccaaatt gatgtaatgg cacccgattg gtgtcgtccg cttttagccc
     9721 gtatgcctga ggtgcgtaat gcgatttcaa tgccgattgg acacggttca tttcgtttgt
     9781 gtgaacgcta ccgactcgga aaatccctga gaaatcaata tgatatggcg attgtattgc
     9841 ctaattcatt aaagtctgcc tttattccac tgtttgcgaa aattgcgaga cgtcgtggtt
     9901 ggaaagggga aagtagatat tttttcttaa atgatcttcg cagtaataaa aacgactacc
     9961 caatgatggt gcaacgctat gtagccttgg cgtttgaaaa ggctactgtt ccaaccgcac
    10021 aacagttgcc gattgcttat ccatacctca caactgatag cgaacaaatt gagcagacca
    10081 aagcgaaatt tgaaaaacag ttggcttatg cggaaaaccg tcctgccatc ggcttttgtc
    10141 cgggggctga atttggaccg gcaaaacgtt ggccgcatta tcattatgca acattggcaa
    10201 aaatgttgat tgagcaaggc tattcggtac ggttatttgg ctcgaaaaaa gatgaagccg
    10261 ccggtgagca aattcgcttg gcattacctg aaaatttaca acgctattgc ctgaatttag
    10321 ccgggcaaac cgatttaaac caagcggtag atttgattgc agattgtaat gcagtcgtga
    10381 gcaatgactc cggcttaatg cacattgccg cagcactaaa taaaccgctg gtcgccttat
    10441 atggaccgac cagcccacaa tatacgccgc cgttatccca aaatgccgtg atcattcgct
    10501 taattgaagg cggtttgatt aaaatccgca aaggcgaagg ggcagagggc tatcaccaaa
    10561 gtttaattga tattcagcct gaaatggtga tggacaaatt agctgaactg ttaatttaaa
    10621 tggataacaa gcggttgaat tttgccaatt ttttgcaaaa aaagaccgct tgtttgttta
    10681 taaaagatga aaattttaat cgttaaaacc tcatcaatgg gagatgtatt acatacgctt
    10741 ccagccataa ccgatatgca aaatgctatt cccgatttaa aagtagattg ggtagtggag
    10801 gagaatttta ccgaaattcc tgcgtggcat tcagcggtaa ataaagtaat tccggtggct
    10861 attcgccgct ggcggaaaaa cctttgccaa agacagactt gggtagaatg gcagcagttt
    10921 ttaaaacagc taaaaagtga gcagtatgat gcggtaattg atgctcaagg gctgattaaa
    10981 agtgcggtgt tagtaactcg ccaagccaaa ggcaaaaaat ttggctatga taaaaactct
    11041 gcccgagaag ggttaagtag tctgttttac gatgaaacgt ttaatatccc ctaccaacaa
    11101 cacgctgtag aacgcatcag aaaattatgt gcaaaatcat taggttatgc tttgccaacc
    11161 gaacggggcg attattgcat tgcacaacat tttgcaaaaa atcctcaaaa aacgaccgct
    11221 tacattatgc tgattcacgc cactacccga gcggataaac attggcaaga agcctattgg
    11281 gcggagttga ttcaagcgtt ggataagcac aatctggcag tcagattgcc ttggggaaac
    11341 caagccgaaa aagaacgggc ggagcgttta gcgagtgtgg cgaataatgc taaggtattg
    11401 ccaaaaatga gcttaaccga tattgctact caacttgcgg gagcagcggc agtggtgtcg
    11461 gtggataccg gcttgagcca tttggcggct gcgttagata agccaaatgt gattttatat
    11521 ggggcaaccg atccaaaact tatcggggct tacgggcaaa atcagtttta tctaaaagca
    11581 gataaaatgg aaaatattac ctcttcacaa gtgctggaaa aattacttcc attgattaac
    11641 aaatgatcgt tgttgttatc ttactgtgct tgacttttat ccacattcaa tttaaaattt
    11701 gccgtccttt cgtgtgaaag gatgttttta attttatttt taataatttt aactggagct
    11761 tttttaatgg caactatcaa ccagctagta cgcaaaccgc gtgtgaaaaa ggttgtaaaa
    11821 agtaacgttc ctgcattaga ggcttgcccg cagaaacgtg gcgtgtgtac tcgtgtatac
    11881 actactacac ctaaaaaacc gaactcagca ttacgtaaag tatgtcgtat tcgtttaaca
    11941 aatggctttg aagtaacttc atacatcggt ggtgaaggtc acaaccttca agaacacagt
    12001 gttgtattaa tccgtggtgg tcgtgttaaa gacttaccgg gtgttcgtta ccatactgta
    12061 cgtggtgcac ttgactgtgc aggcgttaaa gatcgtaaac aaggtcgttc taaatacggc
    12121 gttaaacgtc ctaaagctta atggttctcc gttgagtaag gccaaacgtc taaattttaa
    12181 tatttaaacc ataaactcca gtcaagatgc cttaaggcag attagatgag ttttggatat
    12241 cctgaagatt taatatacgg agaaatttcc aatgccacgt cgtcgtagta ttgaaccacg
    12301 caaaattctt ccagatccga agttcggttc agaattactt gcaaaattta ttaacgtgtt
    12361 aatggtagat ggtaaaaaat ctactgcaga aaaaatcgtt tacggtgcat tagaaacttt
    12421 agcacaacgt tctggtaaag aagcgttaga agctttcgaa atcgcattag aaaacgtacg
    12481 tccaactgtt gaggttaaat ctcgccgtgt gggtggttct acttaccaag ttcctgtaga
    12541 agttcgccca acccgtcgta atgctttagc aatgcgttgg atcgtagaag ccgctcgtaa
    12601 acgtggtgac aaatcaatgg cattacgttt agctaacgaa ttatcggatg cagcagacaa
    12661 caaaggttca gcagtgaaga aacgtgaaga cgttcaccgt atggctgaag ctaacaaagc
    12721 gtttgctcac ttccgttggt aatcgtttaa gtaagcttat agaaggcttc atctcagttt
    12781 gcggtgaagc cttaccctta ttaagaattt taatttaaac cccaaacaag gtaataaata
    12841 aaaatggctc gtacaacccc tatttcactt taccgtaaca tcggtatcag tgcgcacatc
    12901 gatgcgggta aaacaactac tacagagcgt atcttgttct atacaggtgt aagtcacaaa
    12961 atcggtgaag ttcacgatgg tgctgcaaca atggactgga tggaacaaga acaagagcgt
    13021 ggtatcacga tcacatctgc tgcaacaaca gcattctggt ctggtatgtc acaacaatat
    13081 ccacaacacc gtatcaacgt tattgatact ccgggacacg ttgactttac tatcgaagta
    13141 gaacgttcaa tgcgtgtact tgatggtgcg gtgatggttt attgtgcagt tggtggtgtt
    13201 caaccacaat ctgaaactgt atggcgtcaa gctaacaaat ataaagtgcc acgtatcgcg
    13261 ttcgtaaaca aaatggaccg tacaggtgca aacttcttac gtgttgttga gcaaatcaaa
    13321 acgcgtttag gtggtaactc agttgcatta caacttccaa tcggtgcaga agacagcttc
    13381 aaaggtgttg ttgatttaat caaaatgaaa gcaatcaact ggaacgaagc tgatcaaggt
    13441 atgaccttca cttatgaaga tattccggca gatatgcttg aagcttgtga agaacaccgt
    13501 gcaatgcttg ttgaagcagc agctgaagct tctgaagaat taatggaaaa attcttctct
    13561 ggtgaagaat taactgaaga tgaaatcaaa gcagcattac gtcaacgtgt attagcaggt
    13621 gaagtaatcc ctgtttgctg tggttctgca tttaaaaaca aaggtgtaca agcaatgctt
    13681 gatgcggtaa tcgattactt accggcacca actgatattc ctgcaattaa aggtatcaat
    13741 gaagatgaaa ctgaaggtga gcgtcacgca agcgacgatg agccatttgc ttcattagca
    13801 ttcaaaattg caacagaccc attcgtaggt aacttaacct tcttccgtgt ttactctggt
    13861 gtaattaact ctggtgatac agtttacaac tcagtaaaac aaaaacgtga acgttttggt
    13921 cgtatcgtac agatgcacgc aaacaaacgt gaagagatca aagaagttcg tgcgggcgat
    13981 atcgctgcag caatcggttt aaaagatgtt ggtacaggtg acacattatg tgcaatcgat
    14041 gcaccaatca ttcttgagcg tatggaattc cctgagccgg taatctctgt tgcggttgaa
    14101 ccaaaaacca aagctgacca agagaaaatg ggcttagctt taggtcgttt agcacaagaa
    14161 gacccttcat tccgtgttca cacagatgaa gaatctggtg aaacgatcat ttcaggtatg
    14221 ggtgagttac acttagacat catcgttgac cgtatgaaac gtgagtttaa agtggaagca
    14281 aatatcggta aaccacaagt atcttaccgt gaaactatcc gtactcgtgt taatgatgtt
    14341 gaaggtaaac acgcaaaaca atctggtggt cgtggtcaat atggtcacgt tgtgatcgac
    14401 ttatacccat tagatgctga aggtccgggt tatgagtttg taaacgagat caaaggtggt
    14461 gtaatccctg gtgaatacat tcctgcggtt gataaaggta tccaagaaca gcttaaatct
    14521 ggtccgttag caggttaccc ggtagtagat ttaggtgtgc gtttacactt cggttcatac
    14581 cacgatgttg actcatctga acttgcgttt aaacttgctg catcattagc atttaaagcg
    14641 gcgttcgcaa aagcaagccc ggtattactt gagccaatca tgaaagtgga agttgaaact
    14701 ccaccggatt acgtgggtga tgtaatcggt gacttaagcc gtcgtcgtgc aatggttaac
    14761 ggtcaagaag cgaacgagtt cgttgttaag atcaatgctg aagttccact ttcagaaatg
    14821 tttggttatg caacagactt acgttcacaa actcaaggtc gtgcatcata ctctatggaa
    14881 cctttaaaat atgctgaagc accgaaaaac gttgctgatg caattattga agctcgtaga
    14941 aaataatttg tttaacctga gccatagcaa tagggctatg gctcattttt taggaagata
    15001 ttaaagtgtc taaagaaaaa tttgaacgta caaaaccgca cgttaatgtg ggtacaatcg
    15061 gccacgttga ccatggtaaa acaactttaa cagcagcaat cactaccgta ttagcaaaac
    15121 acttcggtgg tgcagctcgt gcatttgacc aaatcgataa cgcgccagaa gaaaaagcgc
    15181 gtggtatcac tatcaacact tcacacgttg agtacgatac agcaactcgt cactacgcac
    15241 acgttgactg cccgggacac gcggactatg ttaaaaacat gattaccggt gcggcacaaa
    15301 tggacggtgc tatcttagta gtagcggcaa cagacggtcc aatgccacaa actcgtgagc
    15361 acatcttatt aggtcgccaa gtaggtgtac catacatcat cgtattctta aacaaatgcg
    15421 atatggtaga tgatgaagag ttattagaat tagttgagat g
//
LOCUS       DANJWC010000045        14808 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.045, whole genome shotgun sequence.
ACCESSION   DANJWC010000045 DANJWC010000000
VERSION     DANJWC010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14808
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.045"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..441)
                     /locus_tag="PWX02_001030"
     CDS             complement(<1..441)
                     /locus_tag="PWX02_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL1729056.1"
                     /translation="MFSDNKALHLGKIYHLIEQFELISRTDLAKLSGLAPASVTNLTK
                     ILIDNHFILERTVQTTLSRGRPSVGLAVSNFFWQLLCVTVSSHKIEISLCKLNGAEIY
                     KQDYTISAENYPRLNDFILNFITKFRQLYCVNTSQILAVSISVIG"
     gene            575..1693
                     /gene="rnd"
                     /locus_tag="PWX02_001031"
     CDS             575..1693
                     /gene="rnd"
                     /locus_tag="PWX02_001031"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HDL1729057.1"
                     /translation="MESLIHYQWVETNEQLEAVCEQARKTKVVALDTEFIRTRTYYPI
                     LGLIQLFDGQQVSLIDPNTISDFSPFVSLLADKNIIKVLHACSEDLEVFEYQFKQLPE
                     PMLDTQVMAAFAGIGISMGFAKLVSHYLNIELDKGASRTDWLARPLSAEQLQYAAADV
                     WYLLPVYEKLAQALTKSTWQSAVAEECVALLKKAKQPINKEKAYKNIANAWRLNREEL
                     AILQVLAKWRIEEAQKRNLALNFVVKEASLVQIAKSCPKHTSSLLEFMHPNEVRIHGK
                     KILWLVEQGKAIESNDYPELIQRLVDEKGYKYYMQAMLQKLLEIQPLDIVPELVASKR
                     QLNQLFKWYIDGKPSEKIPELLIGWRKPFGEQLLSVLS"
     gene            1750..2232
                     /gene="lrp"
                     /locus_tag="PWX02_001032"
     CDS             1750..2232
                     /gene="lrp"
                     /locus_tag="PWX02_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005695827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HDL1729058.1"
                     /translation="MENKKPPKALDAIDLKILNELQRNGKISNIELSKRVGLSPTPCL
                     ERVKRLEKNNVIMGYKALLNPELLDAPLLVIVEITLVRGKPDVFEEFNKAIQQLDEIQ
                     ECHLVSGDFDYLLKTRVADMASYRKLLGTTLLRLPGVNDTRTYVVMEEVKQTNYLLLK
                     "
     gene            2243..4903
                     /locus_tag="PWX02_001033"
     CDS             2243..4903
                     /locus_tag="PWX02_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HDL1729059.1"
                     /translation="MIEQLKPHLKGKENLIKLMLLLTGFLGVYLLIAWASYSPLDNAW
                     SVASSITQTTLNKTGHFGAWSIDMLYAMFGKVAVLVPIALIIFSIYSLGMGLSVDMKW
                     KTVFIRLASFLLLMVGLAGLFSVLFSNSAYYLSGGFVGGVWETILSDTIGQFGALLIA
                     MLFTIAGLYFCSVQSLLPILTQFYEWIMAKSEESKTAESLQHLQNTEQKQPLVTENEA
                     VEVVDDHKPQFTDISAFKRPNIGGLRHSENVEIDNREMYEIERDLELPTINILIQPDM
                     VNQVAEQATSGYELPNEESNVLQMPTIRLNTAIENEVDTIENSSSLEDLKIAESMLPQ
                     TQLDNDIEDTEEAIDDVREITPEFMVTPPKAMKDVSEQPAYPKGYGDTLIHPLLQRKV
                     ITEKPTTPLPTLDLLDKAPIQSQQITEQEIRETSVRLEAELANFGVKATVEDVLVGPV
                     VTRYEIQPAAGVKASKITNLASDIARGLMFKAIRITEVIPNKPYMGIETPNKHRETVW
                     LRDVLDSDEFRNTTATLPMALGKDISGEPVVVDMAKMPHLLVAGQTGGGKSVGVNTMI
                     LSLLFKLTPEQVRFIMIDPKVVELSIYNDIPHLLTPVVTDMKKAANALRWAVEEMERR
                     YLLVSHLQVRNIEGYNAKIDQAAAMNLPIPDPTWRPGDSMDSLPPPLQKLSYIVLIVD
                     EFADLMMSAGKEVEEYIMRIAQKARAVGIHLILATQRPSTDVITGVIKANIPSRIAFT
                     VASQIDSRTILDSGGAEALLGRGDMLYSGAGSPDIIRIHGAFMKDEEVQRVADNWRAR
                     RKPNYLESIVESRSEEADGKNDGGTGDLDPLFDEVVEYITETGSVSISNIQRRFSLGF
                     NRAARIVDQMEAQGIVSEPLKGGKREVLAR"
     gene            5055..5894
                     /locus_tag="PWX02_001034"
     CDS             5055..5894
                     /locus_tag="PWX02_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HDL1729060.1"
                     /translation="MRLDDIKESKNVEDRRFNNVGRTKVGRGKGGILTFIIVLVGAYY
                     GVDLTGLMGGGVEYEEQTSALNESEEKQLRTLSAKVLTTTENIWTSYFQQNGLTYKEP
                     SLVLYRGAIQTACGTGQSAMGPFYCPVDQKVYLDLSFYDDMRKKLRASGDFAFSYVIA
                     HEVGHHVQNLLGITSKTQRAQMKASSKAEANQISVNVELQADCFAGVWGHQLAKANRL
                     EEGDVEEAFNAAQAVGDDRLQQQSRGYVVPDSFTHGSSAQRLAWFRKGLTTGNPAVCN
                     TFN"
     gene            5983..7065
                     /gene="aroG"
                     /locus_tag="PWX02_001035"
     CDS             5983..7065
                     /gene="aroG"
                     /locus_tag="PWX02_001035"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HDL1729061.1"
                     /translation="MSYIKNDDVRITSIEELLPPIALLEAYPASDVAAETVAKTRQSI
                     HKILHGADDRLLVVIGPCSIHDPKAALEYAQKIKAMRADPKINQNLEVVMRVYFEKPR
                     TTVGWKGLINDPYLNETFALNDGLRIARKVLSDINDLSVPTAGEFLDMITPQYVADFM
                     SWGAIGARTTESQVHRELASGLSCAVGFKNGTNGSVKIALDAISSAKSPHHFLSVTKF
                     GHSAIVSTAGNPDCHIILRGGDSATNYDADSVAAACAAIEKSGGRPHVMIDFSHANSQ
                     KQFKRQMDVCADVCNQIAGGSDFISGVMIESHLVEGRQDLIEGKGLEALVYGQSITDG
                     CIGWEDSEKALFALAQAVEKRRQFAK"
     gene            7148..7840
                     /gene="tsaB"
                     /locus_tag="PWX02_001036"
     CDS             7148..7840
                     /gene="tsaB"
                     /locus_tag="PWX02_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDL1729062.1"
                     /translation="MKSTILALDTATEACSVALQHNGNITFLEEISPRSHTQRILPMV
                     DELLHQANIELKEVNYLVFGRGPGSFTGVRVGVSVAQGLAMGANLSVVAVSNLKAMAE
                     EAYQKLGATKVIALIDARMNEVYFAQFERKGAEWNEVVAEQVCSPEAAIGQFQVSEET
                     VVVGTGWAAYLQFSEQNLPLVISDITLPSSRYMLSIAETEILKGNTLSALEIEPVYLR
                     NEVTWEKLPHKQ"
     gene            complement(7855..8652)
                     /locus_tag="PWX02_001037"
     CDS             complement(7855..8652)
                     /locus_tag="PWX02_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005636253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL1729063.1"
                     /translation="MTMAVIIILIICGVVVNITSALFGIGGGVLMMPILHSLFPELPL
                     QVISACSLTIVIGSATINLCLFHHQQIKINRKNVLIWSLAMMLGVQIGFELSFLFSDN
                     SIIAIFILTLLTLSFKTLFHKPRVKPKPKEAVSNATMCSLGGLIAGITGIGGGSIMAP
                     IVTQIRSVSPKQVAVYTNYMMVIGGIGNLYGYLSRPFVLNSTLATWQIGYVNFAIVGI
                     VVMSSLFTVYFSMKLRNILSPELTTKLLGMILFGIACYTGFLQYFFI"
     gene            8751..9302
                     /locus_tag="PWX02_001038"
     CDS             8751..9302
                     /locus_tag="PWX02_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL1729064.1"
                     /translation="MNNMPTPEQLMSCQLSVMSDLEKGVVIYSQGYHAREFYFLKSGL
                     IGLYHMLENGKESLVRVYKACDYFGFRTFFGNSTYHCSARVLQPAELIRITPLNFMEF
                     WANNSDLIISLLKQLSTELQDAEHRLSMVSYQKTQERVISSIHYLHQRYPNYHWTNRE
                     IAEFAGCETETAIRLRRELKKIS"
     gene            9446..11143
                     /locus_tag="PWX02_001039"
     CDS             9446..11143
                     /locus_tag="PWX02_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HDL1729065.1"
                     /translation="MLLTIITFLVVTGLVGYISWQKTRNDDLSTSKGYFLAGRGLSAT
                     IIGCSMVLTSLSTEQLIGVNANSYAGNFSIIAWTVPAVIPLCFLALYLLPKYIRNGYT
                     TIPEFFENRFDRQTRLIMSGLFLVFYLFIVIPTALYTGAIAFNKIFNLETAFGLSYGA
                     AITYTVIAIGIVGAIYAIFGGLKAVAVSDTWNAVLLVIGALLVPVFALIYLGNGSMAE
                     GVKIITTTHVEKWNAVGSATDAVPWPTIFTGIMVVHFFYWTTNQAIVQRALGARDLKA
                     GQKGILIAALFLLLLPLILNLPGLLSFHILGEGLKPIDVSYPELVNKTFPTVLQGFFI
                     AALFGAILSTFNSFLNSAATIYCKDLLPSISKKQFTDAELIRYAKKVSAVMAVITMIF
                     GPLLMFGSDGIFLLTKRFAGFVNIPIVALFAVGLFNKTVSGKAARIALLAHVILYFCI
                     VWVFDVKINFVYVQAGLFVFDVALMLILGTFLKRATPYEDNKINHANVDLTGWEYAPL
                     AVASIVLGLFTLYAFLSPLGLASPDGNPAMVLAVYAVLQAVVCVVFFAKCKGKKVVN"
     gene            11235..12683
                     /locus_tag="PWX02_001040"
     CDS             11235..12683
                     /locus_tag="PWX02_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDL1729066.1"
                     /translation="MNNIIYILLDQVRKDMLGTYGHQVVKTPNLDRLAKDGIRFNNAF
                     TPASVCGPARTSLFTGLMPSSHGIIKNGEKGGTGEIPQQNPNIAGVEGYNSFVIGKWH
                     VGTKSVPEDYGIAGHNFDGYGYPGSQVYKNLVFNQPPTHSNRYREWLAEKGYDAPEVS
                     RAYFGDNPHLRVQELCGLLSGSREQTIEWFIIDEAKKYIQASLDEKKPFFAWINFWGP
                     HTPCVVPEPYYSMYDPSQVVLDESFFKPLEGKPNHFRTISQMWGMWEASEDRWKEVIS
                     KFWGYITLIDDAIGELFRFLEENNLYENTFLVATADHGDAMGAHKMIEKGEFMFETTY
                     NIPLIIKDPHSTRVNEVDDNLVYLHDLTSTVYDVANQPIPTAFQGESILPITRQSQNN
                     DRKGLLCQLAGHFVYFEQRMWRRKDYKLVFNATDRCELYDVRKDPEEMHNLFYDEAYR
                     AVKKEMLEEMRQEMKRLNDPLENWVYRIIDEI"
     gene            12773..13891
                     /locus_tag="PWX02_001041"
     CDS             12773..13891
                     /locus_tag="PWX02_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDL1729067.1"
                     /translation="MTTTYFHLMAKPSSFHCNIQCEYCFYLSKEQTIPPEKSQFMNDE
                     TLQNYIRHYIEANQSQRVDFVWQGGEPTLLGLDFYKQAVKYQQKFANGKQITNAIQTN
                     GIALNRQWASFFKENHFLVGLSIDGLSDIHDRYRISTNGKPTFERVKSAVDLLKEVGV
                     EFNTLTVVNDRNWQRGRETYQALKALGSRYMQFIPVVESHKGELQPFSVPPQGYGQFL
                     VDVFHEWLPDRGQIFVIEFDNLLGQWLGYPSNNCVFRPTCGSSLVVEACGDVYSCDHF
                     VYPDYHIGNLNQQQLAEIVSSAAQRQFGQNKQAKLSQACLNCKFRILCHGGCPKHRIV
                     PQANSENHNYLCPSYQQFFAETSSVMRQMSEQIRFAGK"
     gene            complement(13982..14563)
                     /locus_tag="PWX02_001042"
     CDS             complement(13982..14563)
                     /locus_tag="PWX02_001042"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014064645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDL1729068.1"
                     /translation="MAKLYFYYSSMNAGKSTTLLQSSYNYQERGMNTLVYTAAIDDRF
                     GVGKVNSRIGISQEANLFTADTNLFAEIKALHSNKALHCILIDEAQFLTKTQVYQLSD
                     VVDKLKIPVLCYGLRTDFQAELFEGSKYLLAWADELEELKTICDCGKKAHFVVRLNEK
                     GEAIKDGEQIQIGGNDRYLSVCRYHYKQKLGKL"
     gene            complement(14619..>14808)
                     /gene="zapB"
                     /locus_tag="PWX02_001043"
     CDS             complement(14619..>14808)
                     /gene="zapB"
                     /locus_tag="PWX02_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HDL1729069.1"
                     /translation="EKIKQAVETIQLLQLEVEELKGKNDAAKQENDTLRSEYEQLKVE
                     QQNFQDRLRSLLGQIDNV"
BASE COUNT         4440 a         2942 c         3280 g         4146 t
ORIGIN      
        1 accgatcaca ctaatagata cagccaggat ctgactagta ttgacacaat atagttggcg
       61 gaactttgta ataaagttaa gaataaaatc atttagccta ggataatttt ctgctgagat
      121 tgtataatct tgcttataaa tttctgcacc attgagttta caaagtgaaa tttcaatttt
      181 atgagatgaa acagtaacac aaagtagttg ccaaaaaaaa ttagaaacag ctaatccaac
      241 ggaggggcga cctctggata aagtagtctg aaccgtacgt tctaaaatga agtggttatc
      301 tattagtatt ttagtcagat ttgttactga agctggggct aaccccgata gtttagctaa
      361 atctgttctt gaaatgagtt caaattgctc aattaaatgg tatattttac ccaaatgaag
      421 ggctttgttg tcagaaaaca tttaaaatcc ttatgttaga atttttagat gacaattatt
      481 ttttgatatg aaaaaaataa caatcttttt ttgtttataa tgagagacag atcacataat
      541 tattttttac gtttaaatat ttcaggaaaa gtatatggag tctttgattc actaccaatg
      601 ggttgaaaca aatgagcaat tagaggccgt atgtgaacag gccagaaaaa caaaagtagt
      661 tgccttagat acagagttta tccgtacacg tacctattat ccgatattgg gtttaattca
      721 actctttgat ggccaacagg ttagccttat tgatccaaat acgatttctg acttctcgcc
      781 ttttgtgtca ttattagccg ataaaaatat cataaaagta ttacatgcct gtagtgagga
      841 tttagaagtt tttgagtatc aatttaagca attgccagag ccgatgttgg atactcaggt
      901 gatggcagcc tttgccggca ttggtatttc gatggggttt gcaaaattgg tttcccacta
      961 cttaaatatt gaactagaca aaggcgcttc ccgaaccgat tggcttgcca gaccgctaag
     1021 cgctgaacaa ttacaatatg cggcagctga tgtttggtat ttattgccag tttatgagaa
     1081 attggcacaa gcattaacaa aaagcacttg gcaaagtgct gtagcagagg agtgtgtcgc
     1141 tttgttgaag aaagcgaaac agcctatcaa taaggaaaaa gcctataaaa atattgccaa
     1201 tgcttggcga ttgaatcggg aagagttagc catattacaa gtattagcga aatggcggat
     1261 tgaagaagca caaaagcgca atcttgcctt aaattttgtc gtgaaggagg caagtttagt
     1321 tcaaatcgca aaaagctgtc ctaaacacac ttcttcgctg ttagaattta tgcacccaaa
     1381 tgaagtcaga attcacggaa aaaaaatact atggttggtt gagcaaggaa aagcaataga
     1441 atcgaatgat tatcctgaac tcattcagcg cttggtggat gaaaagggtt ataaatatta
     1501 tatgcaagcg atgttgcaaa aattattaga aattcaaccg cttgatatcg taccggaatt
     1561 ggttgcaagt aaacgtcaat taaatcagct atttaaatgg tatattgatg gaaaaccatc
     1621 agaaaaaatt cctgaattat taatcggttg gcgaaaacct tttggtgaac agctgctatc
     1681 ggttttatct taaatttcat tcgggttaca tagactatgt acagtataat tgcctaaaaa
     1741 ggagtttata tggaaaataa aaaaccacca aaagcattag atgcgattga tttaaaaatc
     1801 ttaaacgagc tacaacgtaa cgggaaaatt tctaatattg aattatcaaa gcgagttggg
     1861 ctttctccaa cgccctgctt agaacgggta aaacggctag aaaaaaataa cgtaattatg
     1921 ggctataaag ctttacttaa ccctgaatta cttgatgccc ctttactggt gattgttgaa
     1981 attactttgg tgcgtggtaa gcctgatgta tttgaagaat ttaataaagc gattcaacaa
     2041 cttgatgaaa ttcaagaatg ccacctggta tcgggggatt ttgattatct gttaaaaacc
     2101 cgtgtggcgg atatggcatc gtaccgtaag ttattaggca caacattgct tcgtttgccc
     2161 ggcgtaaatg acactcgaac ctatgtggtg atggaagaag taaaacaaac caactatttg
     2221 ttgttaaagt aaggagaatt gggtgattga acaacttaaa ccacacctaa aaggcaaaga
     2281 gaacttgatc aaacttatgc ttttgttgac cggctttttg ggcgtttatt tgctaattgc
     2341 ttgggcaagt tatagtccat tagataatgc ttggtctgtt gcaagcagca ttacacaaac
     2401 aaccttgaat aaaacaggtc actttggtgc ttggagtatt gatatgctct atgcaatgtt
     2461 tggtaaggtt gcagttttag tacctattgc attaattatt ttctcaattt atagcttggg
     2521 aatgggttta agtgtcgata tgaaatggaa aactgtcttc attcgcctag ccagtttctt
     2581 attattgatg gtcggtcttg ccgggttatt tagcgtgctt ttttcaaact cagcttatta
     2641 tctgtcgggt ggctttgtcg gtggagtttg ggagacaata ttatctgata ctatcggaca
     2701 atttggtgcc ttattgattg caatgctttt tactatagct ggcttatatt tttgctctgt
     2761 tcaatcatta ttgccaatat taacgcaatt ttatgagtgg ataatggcaa aatcagaaga
     2821 gagcaaaaca gctgaatctc ttcagcattt gcaaaatact gagcaaaaac aaccgcttgt
     2881 aactgaaaat gaagcggtgg aagttgtaga tgaccataaa ccgcaattta ccgatatctc
     2941 cgcctttaaa cgcccaaaca ttggtggttt aagacactcg gaaaatgtag agatagataa
     3001 ccgagaaatg tatgaaattg aaagagatct tgagctaccg actattaata ttttaataca
     3061 gcctgatatg gttaaccaag ttgcggaaca agctacatcg ggctatgagt taccaaacga
     3121 agaatccaat gtattgcaaa tgccgaccat tcgcttaaat accgctatcg aaaacgaggt
     3181 ggatacaatc gaaaacagtt catcattgga agacctgaaa atagcagaat ctatgttacc
     3241 tcaaactcag cttgataatg atattgaaga tactgaagaa gctattgacg atgtgcgtga
     3301 aattacaccg gaatttatgg taacgccgcc caaggcgatg aaagatgtgt ctgagcagcc
     3361 agcctatcca aaaggctatg gtgatacgtt aattcacccg ttgttacaac gaaaagtgat
     3421 aaccgaaaaa ccgacaacac cattgccaac gctagatttg ttggataaag caccgattca
     3481 aagccagcaa attaccgaac aggaaatacg agaaacctct gtgcggctag aagcagaatt
     3541 agcaaacttt ggcgtaaaag ctaccgttga agatgtattg gtcggtccgg tagtgacccg
     3601 ttatgaaatc caaccggctg ccggggtaaa agcctccaaa attaccaatt tagcgagcga
     3661 tatagctcgt ggcttaatgt ttaaagcgat taggattact gaagttatcc caaacaaacc
     3721 ttatatgggt attgaaactc cgaataaaca ccgtgaaaca gtgtggttgc gtgatgtgct
     3781 agacagcgat gaattccgca ataccactgc aacattgcca atggcactag gtaaagacat
     3841 cagtggtgag cctgtggtgg tcgatatggc gaaaatgcca cacttactgg tggcagggca
     3901 gaccggtggg ggtaaatcgg ttggggtgaa tacgatgatt ttaagtctat tgttcaaact
     3961 tacaccagaa caagtccgct ttattatgat tgaccctaaa gtggtggagc tttccattta
     4021 taacgatatt cctcacttat taacgccggt agtaaccgat atgaaaaaag ctgccaacgc
     4081 actacgctgg gcggtggagg aaatggaacg ccgttatttg ctggtaagcc atttacaggt
     4141 gcgtaatatt gaaggctata atgcgaaaat cgaccaagcg gcagcgatga atttgccgat
     4201 tccagatcca acgtggcgac cgggcgattc aatggatagc cttccgcctc cgctacaaaa
     4261 attaagctat attgtgctga ttgtcgatga gtttgcggat ttaatgatgt ctgccggtaa
     4321 agaagtggaa gagtacatta tgcgaattgc tcaaaaagcc cgagcggtgg gaattcactt
     4381 aattcttgct acccaacgtc cgtcaaccga tgtgattacc ggcgtgatta aagccaatat
     4441 tccaagccgg attgcgttta cagtagcgag ccaaattgac tcaagaacta ttttagattc
     4501 aggtggagca gaggcattat tagggcgagg cgatatgctc tattcaggag ctggcagccc
     4561 ggatattatt cggatacacg gtgcgtttat gaaagacgaa gaagtgcaac gcgtggcgga
     4621 taattggcgg gcaagaagaa aaccaaacta cttggaaagc attgtggaat cccgctcgga
     4681 agaggctgat ggcaaaaacg atggcggaac gggcgattta gatccgctat ttgatgaggt
     4741 tgttgaatat atcacggaaa ccggttcggt ctctatcagt aatattcaac gccgcttctc
     4801 gcttggtttt aaccgtgcgg ctagaattgt ggatcaaatg gaagctcaag gcattgtttc
     4861 tgagccactt aaaggtggca aacgagaagt gctggcgagg taaaacacat acaagcggtt
     4921 agatttctgt gaaattttgc aaattttgac aaaaatctaa ccgcttgtat ttatctaatg
     4981 ctaaataagc atagtataag gctagtcttt gggctaaaat taacgcaatt tttacatctt
     5041 ttaaggttga atatatgcga ttagatgata ttaaagaaag taaaaatgtt gaagacagaa
     5101 gattcaacaa tgtgggtaga actaaggttg gtagagggaa aggtggaata ttaaccttta
     5161 ttatcgtgct tgttggagct tattatggtg ttgacttaac aggtttaatg ggcggtggcg
     5221 tagaatatga ggagcagaca tcagcattaa atgaaagtga agaaaaacag ctaagaacct
     5281 tatccgctaa agtattaacg accacagaaa atatttggac ttcctacttc cagcaaaatg
     5341 gtctaactta taaagagccg tctttggtgc tttaccgtgg ggcaattcaa actgcctgtg
     5401 gcaccggtca atctgcaatg ggtcctttct attgccctgt cgatcaaaag gtttatttag
     5461 atttatcatt ctatgatgat atgcgtaaaa aattaagagc atcgggcgat tttgccttct
     5521 cttacgtgat tgcacacgag gttggccacc acgtccaaaa tttattgggc ataacaagca
     5581 aaacccaacg ggctcaaatg aaagcctcat ctaaagccga agcaaaccaa atttcagtca
     5641 atgtagaatt gcaagctgac tgttttgccg gcgtttgggg acaccaactc gcaaaagcca
     5701 accgattaga ggaaggcgat gtggaagaag cctttaatgc tgcccaagca gttggagatg
     5761 accgattaca gcaacaaagt cgtggctatg tcgtgccgga tagcttcacc cacggctcat
     5821 ctgcacagcg tttggcttgg tttagaaaag gtttaaccac aggaaatcct gcagtttgta
     5881 atacatttaa ttaaaaaata attaaattat ttgaaatctt tttattatcg tactactatg
     5941 atagtacgat tttattttca ttttatttag agagtgaaca caatgtctta tatcaaaaat
     6001 gacgatgtca gaattaccag tattgaagaa ttactaccac ctattgcatt actggaagcc
     6061 tatccggcaa gtgatgttgc agcggaaacc gttgctaaaa cccgtcaatc aatccacaaa
     6121 attttacacg gtgcggatga tcgcttatta gtggtgattg gaccttgctc cattcacgac
     6181 ccgaaagcgg cattagaata tgcacaaaaa attaaagcaa tgcgtgctga tccgaaaatt
     6241 aaccaaaact tagaagtggt gatgcgtgtc tattttgaaa aaccccgtac aaccgttggt
     6301 tggaaaggct taattaacga cccttatttg aacgaaacct ttgccttaaa cgatggttta
     6361 cgcattgccc gtaaagtatt atctgatatt aatgatttaa gcgtgccgac tgccggcgag
     6421 tttttggata tgattacacc acaatatgtg gctgacttta tgagctgggg agcaattggg
     6481 gcgagaacga ctgaatctca agtccaccgt gaattagcct ctggtttatc ctgtgcggta
     6541 ggttttaaaa acggcacgaa tggtagtgtg aaaattgcat tagatgcaat ttcttccgca
     6601 aaatcgccac accatttctt atcggtcact aaattcgggc attctgcgat tgtttcaaca
     6661 gcaggaaatc ctgactgtca cattattttg cgtggtggcg atagcgcaac caactacgat
     6721 gctgactccg ttgcagcggc ttgtgctgcg attgagaaat ccggcggacg tccacacgtg
     6781 atgatcgatt tcagccacgc taacagccag aagcagttca aacgccaaat ggacgtttgt
     6841 gctgatgtgt gcaaccaaat tgcaggtgga tcagacttta tttcaggtgt gatgattgaa
     6901 agccacttgg tagaaggtcg ccaagactta attgaaggca aaggtttaga ggcgttagtt
     6961 tacggacaaa gtattacaga cggttgtatt ggttgggaag atagcgaaaa agcgttattt
     7021 gcactggcac aagcggtcga aaaacgccga caatttgcaa aataatcttt tcattgaggg
     7081 cgaatacaat tcgccctttc tttgtttaac cttaaagcaa attcaatttg ttcaaactaa
     7141 aaatatgatg aaatctacta ttcttgcttt agatactgcc acagaggctt gctctgtagc
     7201 gttacaacat aacggaaata tcactttctt agaagaaatc agccctcgtt cccataccca
     7261 gcgaattttg ccgatggtcg atgaattact tcatcaagcc aatatcgaac tcaaagaagt
     7321 aaattattta gtattcggac gtggtccggg cagttttacc ggcgtgagag taggtgtaag
     7381 tgtggctcaa gggttagcga tgggggcgaa tttgtcggtg gtcgctgttt ccaacttaaa
     7441 agcaatggca gaagaggcat atcaaaaatt aggggcaaca aaggtaattg ccttaattga
     7501 tgcccgaatg aatgaagttt actttgctca atttgaacgc aagggtgcag aatggaatga
     7561 agtagtggct gaacaagtct gttcgcccga agctgcaata ggccaatttc aagtaagtga
     7621 agagactgtg gtggttggca caggttgggc ggcatatttg caattttctg agcaaaatct
     7681 accgcttgtt attagcgata tcactttgcc ttccagtcga tatatgcttt cgattgctga
     7741 aactgaaatc ctaaaaggga atactctatc tgcccttgaa atagaaccgg tgtatttgcg
     7801 taatgaagtc acttgggaaa aattaccgca taagcaataa cactacgctt tttgctaaat
     7861 aaagaagtat tgcaagaacc ccgtataaca agcaatgcca aataagatca tacctagtag
     7921 cttcgtggtt aattcggggc ttaaaatatt gcgcaatttc attgaaaaat agaccgtaaa
     7981 aagcgaactc attaccacaa tgccgacaat cgcaaaattc acatagccga tttgccaagt
     8041 tgctaaggtt gaattcaaca caaaagggcg ggataagtag ccatataaat tgccgattcc
     8101 gccaatcacc atcatatagt tagtataaac tgcaacctgt ttcggtgaaa ccgagcgtat
     8161 ttgcgtgaca atcggagcca taatcgaacc gccgccaatg ccggtaatac ctgcaattaa
     8221 accgcctaaa ctgcacattg tggcattaga tactgcttct tttggtttag gtttaactct
     8281 tggtttgtga aatagggttt taaatgaaag tgtcagcagg gttagaataa agatagcgat
     8341 aatgctgtta tcagagaaaa gaaaactgag ttcaaaccca atttgtacgc ctaacatcat
     8401 cgcaagtgac caaatcaaca cattttttcg attgattttt atctgttgat ggtggaataa
     8461 gcataaattg atagtggctg aaccaatcac aatggtaaga gagcaggcgg aaatcacttg
     8521 gagaggcagt tcaggaaaca gtgaatgtaa aatcggcatc attaaaacac cgccgccaat
     8581 cccaaatagt gcagaagtga tattgactac aacaccacaa atgatcaaaa taattatcac
     8641 ggccattgtc ataatccatc tcctaaagtg cgataagtat gcaaaatttt ctaaataaag
     8701 gattatgatc gcaatcatat atggcaaaaa taaaataata aggataatca atgaacaata
     8761 tgccaacccc cgaacaattg atgtcttgcc aattaagtgt aatgtctgat ctagaaaaag
     8821 gagtcgtcat ttattctcag ggttatcacg ccagagaatt ttatttttta aagagtggct
     8881 tgatagggct gtatcatatg ctggaaaatg gtaaagagag cttagtaagg gtttataaag
     8941 cctgtgatta ctttggtttt cggacctttt ttggtaacag cacttaccat tgttcagccc
     9001 gagttttgca gccggcagaa ctgatacgaa ttaccccatt aaattttatg gagttttggg
     9061 caaacaattc tgacttaatt atctccctat taaaacagct ctctacagaa ttacaagatg
     9121 cagaacatcg tttatcgatg gtgtcctatc aaaaaacgca agagcgggta ataagcagca
     9181 tccactattt acatcaacgt taccccaatt atcactggac taaccgtgaa atcgctgaat
     9241 ttgcaggttg tgaaaccgaa acagcaattc ggttacggcg ggaattaaag aaaatcagtt
     9301 aaattgtcat aaattattca tagttgtgat caccatcaca tatctattat gacaaacatc
     9361 atattctatc gttaaaaata agatttatgc tattgccgtc tctaatggga tatgtcattt
     9421 atatagtcat attgaggtga agaatatgtt attaacaata attacttttc tggttgtgac
     9481 tggactagtc gggtatatat cctggcaaaa aaccagaaat gacgatcttt caacatctaa
     9541 aggttatttc ttggcagggc gtggcttgag tgctacgatt atcggctgtt caatggtctt
     9601 aacttccctt tctaccgagc aacttatcgg cgtgaatgcc aactcttatg ccggtaattt
     9661 ctcgattatt gcgtggactg tgccggcggt cattccgctt tgcttcttgg cactttatct
     9721 gttgccgaaa tatatccgca acggttacac caccattccg gagttctttg aaaaccgttt
     9781 cgaccgccaa acccgtttga ttatgtcggg cttgtttttg gtgttctatc ttttcatcgt
     9841 cattccgacc gcactttaca ccggtgcgat tgccttcaat aaaatcttca accttgaaac
     9901 cgctttcggc ttaagctacg gggcggcgat tacctacacg gtgattgcga ttggtattgt
     9961 tggggcgatt tatgcgatct tcggtggctt aaaagcggtg gcggtgtcag acacttggaa
    10021 tgcggtcttg ttagtcattg gggcattatt agtgccggtg tttgcactta tctatttagg
    10081 taatggcagt atggcagaag gggtgaaaat catcaccact actcacgttg aaaaatggaa
    10141 tgcggtgggt agtgccaccg atgcagtgcc gtggccgacc attttcaccg gtattatggt
    10201 ggtgcatttc ttctactgga cgaccaacca agccattgtg caacgtgcct tgggggcgag
    10261 agacttaaaa gccggtcaaa aagggatctt aattgcggca ctgttcttat tattgctacc
    10321 gcttatctta aacttaccgg gcttactcag cttccatatt ttaggtgagg gcttgaaacc
    10381 gattgatgtg tcttaccctg aattggtgaa taaaactttc ccaaccgtgc tacaaggctt
    10441 ctttattgcc gcactgttcg gggcgatttt gagtaccttc aactcgttct taaactcagc
    10501 ggcaaccatt tactgtaaag acttgctacc gtctatcagc aaaaaacagt tcacagatgc
    10561 ggaacttatc cgctatgcga aaaaagtctc tgcggtaatg gcggtgatta cgatgatctt
    10621 cggaccgtta ctgatgttcg gttcagacgg tattttcttg ctgaccaaac gcttcgccgg
    10681 ttttgtgaac attccgattg tggcactttt cgctgtgggg ttattcaaca aaaccgtctc
    10741 aggaaaagcc gcacgcattg ccttgctcgc acacgttatt ctctacttct gcatcgtttg
    10801 ggtgtttgat gtaaaaatca acttcgtgta tgtgcaagcc ggtttgttcg tgttcgatgt
    10861 ggcgttaatg ctgattttag gcactttctt aaaacgtgcg accccatacg aagacaacaa
    10921 aatcaaccac gctaatgtgg acttaaccgg ttgggaatac gccccactgg cagtggcttc
    10981 tatcgtattg ggcttattca cgctatacgc tttcctttct ccgctcggtt tagcctcgcc
    11041 agacggcaac ccagcgatgg tgctggcggt gtatgcggtg ctacaagcgg tggtttgtgt
    11101 tgtgtttttt gcaaaatgta aaggtaaaaa ggttgtgaat taaccaaata tacactcccc
    11161 tcttttctaa agaggggagt gtatatttgg aagactattt catcagggct cttgccctaa
    11221 caaagaggtc atttatgaat aacatcatct atattttgtt agatcaagtg cgtaaagata
    11281 tgctcggcac ttacgggcat caggtggtga aaaccccaaa tttggatcgc ttggcgaaag
    11341 acggcattcg gtttaacaac gcctttacgc cggcttcggt ctgcgggccg gctcgcacct
    11401 cgctgtttac cggcttaatg ccgtccagcc acgggattat caaaaacggc gaaaaaggcg
    11461 gcacaggcga aattccacaa caaaacccta atattgccgg cgtggagggc tataactcct
    11521 ttgtgatcgg caaatggcac gttggtacaa aatccgttcc cgaagattat ggcatcgccg
    11581 gtcataactt tgacggctat ggctacccgg gtagccaagt gtataaaaac ctcgtgttca
    11641 atcagccgcc aacgcactcc aaccgctacc gtgaatggct ggcggaaaaa ggctatgacg
    11701 cacctgaagt cagtcgagcc tattttggcg acaatccaca cctgcgtgtg caggaactct
    11761 gtggtttgct ttccggcagt cgcgagcaga caatagagtg gtttatcatt gacgaggcga
    11821 aaaaatacat tcaagcctcc cttgatgaga aaaaaccgtt ctttgcgtgg atcaacttct
    11881 gggggccgca cactccgtgt gtagtgccgg agccgtatta ttcaatgtat gacccaagcc
    11941 aagtggtatt ggacgagagt ttcttcaaac cgcttgaggg caaaccgaac cacttccgca
    12001 ccatttcaca aatgtgggga atgtgggagg cgagcgaaga tcgttggaaa gaggtgattt
    12061 ctaaattctg gggctacatc acgctgattg acgatgccat tggcgagctg tttcgtttct
    12121 tggaagagaa caatctctat gaaaatacct tcctcgttgc taccgccgac cacggcgatg
    12181 cgatgggggc gcataagatg atcgaaaaag gcgagtttat gtttgaaacc acctacaaca
    12241 ttccgctgat tatcaaagat ccacactcta caagggtgaa cgaagtcgat gacaacctcg
    12301 tttatttgca cgatctcacc tccaccgtgt atgatgtagc aaatcaaccg atcccgaccg
    12361 ctttccaagg cgagagtatt ctgccgataa ccagacaaag ccaaaacaac gaccgtaaag
    12421 gcttgttgtg ccaactggca gggcattttg tctattttga acagcgaatg tggcggcgta
    12481 aggattacaa attagtcttc aacgccaccg accgttgtga actctacgat gtcagaaaag
    12541 accccgaaga gatgcacaac ctcttttatg acgaagccta ccgagccgtg aaaaaagaga
    12601 tgttggaaga aatgcgacag gaaatgaaac gcctcaacga cccactggaa aattgggtgt
    12661 atcggattat tgatgagata taattgtttg taggggcggg ttttataccc gcccgaaaat
    12721 taatgtaaac aatgggcgaa cataaagtcc gcccctacaa tttagagata caatgaccac
    12781 cacctacttc cacctaatgg caaaacccag cagttttcac tgtaatattc agtgcgagta
    12841 ctgtttttat ctgagcaagg aacagaccat tccgccagaa aaatcgcagt ttatgaacga
    12901 tgaaacgctg caaaactaca ttcgtcatta cattgaagcc aaccaaagcc aacgggtgga
    12961 ttttgtgtgg caggggggcg agccgaccct gctgggctta gatttctaca aacaagcggt
    13021 caaatatcaa caaaaatttg caaacggcaa gcagatcacc aacgcaatcc aaaccaacgg
    13081 cattgcccta aaccgtcagt gggcgagctt tttcaaagag aatcattttc tggtgggctt
    13141 atccattgac ggactgagcg acattcacga ccgctaccgc atttccacca acggtaaacc
    13201 gacctttgaa cgggtgaaaa gtgcggtcga tttattgaaa gaggttggcg tggaatttaa
    13261 caccctcacg gtggtgaacg accgcaactg gcaacggggc agagaaacct atcaagccct
    13321 caaagccctc ggtagccgtt atatgcagtt cattccggtg gtggaaagcc acaaggggga
    13381 attacaaccc ttttccgttc cgccccaagg ctacgggcaa tttttagtgg atgtttttca
    13441 cgaatggctg cctgaccgtg ggcagatttt tgtgatcgag tttgataacc tgctcgggca
    13501 atggcttggc tatccctcca acaactgtgt attccgcccg acctgtggca gctcgctggt
    13561 ggtggaagcc tgtggcgatg tgtacagttg cgaccatttc gtctatcccg attaccacat
    13621 cggcaacctc aaccaacagc agttggcaga gatcgtctca tcagccgccc aacgccaatt
    13681 cgggcagaat aaacaggcaa aactctcgca agcctgccta aactgcaaat tccgcatact
    13741 ttgccacggc ggctgcccca aacaccgcat cgtcccccaa gcaaacagcg aaaaccacaa
    13801 ctacctttgc ccctcttacc aacagttttt tgccgagact tcgagtgtga tgagacaaat
    13861 gagtgagcaa attcggtttg caggaaagta aagagaggta aataacacaa aaaagcggtt
    13921 aaatttcgct gaaaatttgc aaaaattccc gaaaatttaa ccgcttgctg cttcttgtag
    13981 cttatagctt acctaacttt tgtttgtaat gataacggca aacagagaga tatctatcat
    14041 tgccgccgat ttgtatttgt tcaccgtctt taatggcttc gcctttttca tttaatcgaa
    14101 cgacgaaatg ggcttttttg ccacaatcac agatggtttt gagttcttcc agctcatctg
    14161 cccaagccaa caaatatttg ctaccttcaa ataattcagc ttggaaatcg gtgcgtagcc
    14221 cgtagcaaag caccggaatt ttgagtttat cgaccacatc acttaattga taaacctgtg
    14281 ttttagttaa aaactgagcc tcatcaatca agatacagtg taacgctttg tttgagtgta
    14341 atgccttaat ttccgcaaat agattggtat cagcggtaaa taaattggct tcttgtgaaa
    14401 taccgatacg agagttgact ttccccacac cgaaacggtc atcaatagcg gcggtataaa
    14461 ctaaagtgtt cattccacgt tcttggtaat tataagaaga ttgaagcaga gtagtagatt
    14521 tgcctgcatt cattgaggag tagtaaaaat agagttttgc catagctgta acaaataaaa
    14581 aacggctaaa aatttagccg tttgtgctga aaatagaatt aaacgttgtc gatttgacct
    14641 aaaagtgaac gtaaacgatc ttggaagttt tgttgttcca cttttaattg ttcatattcg
    14701 ctgcgcaagg tgtcgttttc ttgtttcgct gcatcgtttt taccttttaa ttcttcaact
    14761 tctaattgaa gtaattgaat agtttcaaca gcttgtttaa ttttttct
//
LOCUS       DANJWC010000046        14746 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.046, whole genome shotgun sequence.
ACCESSION   DANJWC010000046 DANJWC010000000
VERSION     DANJWC010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14746
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.046"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(79..759)
                     /locus_tag="PWX02_001044"
     CDS             complement(79..759)
                     /locus_tag="PWX02_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729070.1"
                     /translation="MKRRFLFKNYGPDDQTIIQAIQHSVEISRKNNNSNIHIVFPRKS
                     DFESTNLGKIIDQILGAGSAKKLTKGESIGSLDLLLPSRINSSNSSGTILAVYCTSHD
                     MHLIDSNSSADDIIFVSWLPDEANHWENTWHNHGLIIMKGETSLNIPQIPEEIVQSLT
                     NLTAIINLSTGLSHPSDKKHAEQIFKELSQKGYKADEEQIASWAIAHGWNASHLSDLK
                     KLVRRYLS"
     gene            877..1890
                     /gene="cas1c"
                     /locus_tag="PWX02_001045"
     CDS             877..1890
                     /gene="cas1c"
                     /locus_tag="PWX02_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1"
                     /protein_id="HDL1729071.1"
                     /translation="MRKLQNTLYITSQGSYLHKERETLVVEQDRKKVAQLPIHSIGHI
                     FCFGNVLVSPFLMGFCGENNVNLAFFTENGRYLGRLQGRQSGNVLLRRAQYKKSETNP
                     VPVARNIIAAKIQASKRVLQRQIRNHGENADIQLAITALNYSLQQLKIADNLNLIRGI
                     EGDAAARYFGMFSHLIKENSGFHFDGRNRRPPRDGVNALLSFLYSILGKDISGALQGV
                     GLDPQIGFLHADRPGRDSLAQDILEEFRAWWVDRMVLSLINRGQVKPDDFVTEASGAV
                     TLKPEARKLLFQTLQAKKQEKIIHPFLEEEVEIGLLPYIQAMLLARHLRGDLAEYPPF
                     LMR"
     gene            1957..2250
                     /gene="cas2"
                     /locus_tag="PWX02_001046"
     CDS             1957..2250
                     /gene="cas2"
                     /locus_tag="PWX02_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HDL1729072.1"
                     /translation="MLMLITYDISFDDPEGQARLRRIAKHCLDYGVRAQYSVFECDVT
                     PAQWVTLKNKLLATYNPDCDSLRFYHLGSKWRNKVEHHGAKPSVDIFKDTLIL"
     repeat_region   2440..3002
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2440..2471
                     /rpt_unit_seq="gcagccacctacgcgtggctgtgaattgaaac"
     gene            3252..4727
                     /gene="rng"
                     /locus_tag="PWX02_001047"
     CDS             3252..4727
                     /gene="rng"
                     /locus_tag="PWX02_001047"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HDL1729073.1"
                     /translation="MSSTELLVNVTPSETRVALVENGILKELHIEREAKRGIVGNIYK
                     GRVTRVLPGMQSAFVDIGLEKAAFLHASDIVSHTECVDENEKKQFLVKDISELVREGQ
                     DIVVQVVKDPIGTKGARLTTDITLPSRYLVFMPENSHVGVSQRIESEEERARLKSLVE
                     PYCDELGGFIVRTAAEEATEESLKQDVEFLKRLWRKVLERKGKYSAKSMLYGELALAQ
                     RVLRDFIGTHINSVRIDSKLTYEQVREFLAEFMPELTDCVTLYTGQQTLFDAYGVEKA
                     IQKALDKRVDLKSGGYLIIEQTEAMTTIDINTGAFVGHRNLAHTIFNTNIEAIQAIAH
                     QLQLRNLGGIIIIDFIDMQEEEHRERVLQSLQEALKGDRVKTNVNGFTQLGLVEMTRK
                     RTRESLERILCCDCQACQGRGTVKTVETVCYEIMREIVRVHHLFKTENIHVYAAKDVA
                     EYLINEESHALLAELQAFIGKKIEIKLEPYYHQEQFDVVVM"
     gene            4881..6383
                     /gene="lysS"
                     /locus_tag="PWX02_001048"
     CDS             4881..6383
                     /gene="lysS"
                     /locus_tag="PWX02_001048"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDL1729074.1"
                     /translation="MSEVENQELDLNGEMLARREKLNKIREQGNAFPNTFRRDALAKD
                     LHAQYDAVEGETLKEQNIQVKVAGRIMLKRVMGKASFFTIQDVSGAIQLYVARDNLSD
                     SVYEEKVSLWDLGDIVGVEGALFKTKTGELTVRCSEVQLLTKSLRPLPDKHHGLTDLE
                     TRYRQRYLDLISNEESRRTFMIRSQVVSGIRKFFLERDFIEVETPMLQVIPGGAAAKP
                     FITHHNALDVDMYLRIAPELYLKRLVVGGFERVFELNRNFRNEGVSVRHNPEFTMIEY
                     YQAYADYHDLMDNTEELLRQLALDILGTTDVPYGDYVFDFGKPFERITMHDAIVKYGN
                     GIQREDLDSFEKSVEIAKGLGIEIQKSWGLGSVVNAIFEEVAEHQLIQPTFLMAHPAE
                     ISPLARRNDENPEVTDRFELFIGGREIGNGFSELNDAEDQAERFDAQVAAKDAGDDEA
                     MFKDEDFIVALEHGLPPTAGEGLGIDRLAMIFANAPSIRDVILFPAMRQK"
     gene            complement(6461..7534)
                     /locus_tag="PWX02_001049"
     CDS             complement(6461..7534)
                     /locus_tag="PWX02_001049"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HDL1729075.1"
                     /translation="MAQQITLFEDVPLPLTLGGELSPITVAYQTYGRLNAEKSNAVLL
                     CHALTGDAKPYYDSPTEKGWWQDFIGKGLAFDTDKYFFICSNVLGGCKGTTGPASINP
                     KTNKPYGSLFPIITVQDIVNVQKALLDYLEIPQLHAVVGGSFGGMQATQWGISYPDKV
                     RNIINLCSSLTLSAEAIGFNHVMRQAIINDPHFNGGNYYDGIAPDNGLKIARMLGMLT
                     YRTDIQLAKAFGRETKQQGQIWGDHFQVESYLSYQGTKFLGRFDANTYLRLLRALDLY
                     DPALGYENEETALKRIKANYTFVAVTSDQLFKQVDVYKSKQKLEQAGVNLEFHEFHSD
                     FGHDAFLVDYDFFEPMIKAGLAK"
     gene            7802..8485
                     /locus_tag="PWX02_001050"
     CDS             7802..8485
                     /locus_tag="PWX02_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL1729076.1"
                     /translation="MNQTRDRLEKLPLFHKMQQFIIKALKIIAIFPLLGIAILLLVDS
                     LTSYIVRDKIYTDSKQIPYREYAVVLGTAKFYSKDVINQYYKYRLEAAKQLVQEGKVN
                     QLLLSGDNKTPYYNEPKTMTSDLLKMGIATSKIKQDYAGYTTFDSIIRAKKVYKLPPF
                     TIISQKFHCERALFIAKFRDIDAICYAATYPEGAYQVRMREILARSAMVFSLLIGKEP
                     ESLEDIRSK"
     gene            complement(8522..9361)
                     /gene="purU"
                     /locus_tag="PWX02_001051"
     CDS             complement(8522..9361)
                     /gene="purU"
                     /locus_tag="PWX02_001051"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HDL1729077.1"
                     /translation="MLQQHKILLTECPDDTGLVAKITNICYKHQLNIIKNSEFVENET
                     KRFFMRTELNGIFNDETLLTDLKFTLPEGSSYKLLPKQRKRIVILVTKEAHCLGDLLM
                     KNYYGGLDVEIAAVIGNHETLKSLVERFDIPFHLVSHENLTRVEHDKLLAEKIDEYSP
                     DYIVLAKYMRVLNPEFVARYPNRVVNIHHSFLPAFIGAKPYHRAYERGVKIIGATAHF
                     VNDELDEGPIIMQNVINVDHTYTAEAMMRAGRDVEKTVLSQALELVLADKVFVYKNKT
                     IIL"
     gene            complement(9431..9841)
                     /locus_tag="PWX02_001052"
     CDS             complement(9431..9841)
                     /locus_tag="PWX02_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005460020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HDL1729078.1"
                     /translation="MSALKSLLNIRSLRALAKDLALEQLKSISEKLDIIIAEKEEEMK
                     QEELEKLKQLESLNKYREMLKQDGLSIDELAELLADKVIKTRKARKALSPRPAKYKFV
                     DANGNEKTWTGQGRTPKALQDALDSGKKLEDFAI"
     gene            10278..11810
                     /locus_tag="PWX02_001053"
     CDS             10278..11810
                     /locus_tag="PWX02_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HDL1729079.1"
                     /translation="MNLVDYSTSLWSVLPALIALILAIVTRKVIISLSIGILFGAFML
                     TNSNFLDALTYIKNSVVSLVYKGAEEGLNSNNVNIILFLLLLGVLTSLLSISGSNQAF
                     ANWAQKKVKGKRGAKLMAAGLVFFTFIDDYFHSLAVGAIARPVTDKFRVSRAKLAYIL
                     DSTAAPMCVLMPISSWGAYIITLIAGLLATHSITGYSPLGAFMTMSAMNFYAIFAMIM
                     VFFVAYFSFDIGSMARFERQAAHLAELDDEQIETKGHVRDLILPIIALIVATVSMMMK
                     TGADALAESGASFSILGAFENTTVGISLVVGGVAGLIVATICIITSGNISFKNYVRAY
                     GLGVKMMSGAITILFFAWTINGVVSDMQTGKYLSGLVAESIGAEFLPAILFVLAAAMA
                     FSTGTSWGTFGIMLPIGASMAVHSDPALIIPCLSAVMAGAVCGDHCSPVSDTTILSST
                     GAQCNHMDHVISQLPYALLVATASIVGYLVVGFTQSAVLGFIATAIVMAILILLFKEK
                     KH"
     gene            12036..12788
                     /locus_tag="PWX02_001054"
     CDS             12036..12788
                     /locus_tag="PWX02_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL1729080.1"
                     /translation="MKIALVTGVTAGFGLAICKLLLQSGYKVIGTGRRAERLEELHLQ
                     FGDKFLPLAFDIQQPDEVEKALNSRPKAWQQIDLLVNNAGLALGLEPAQKADLNDWYQ
                     MIDTNIKGLVTMTRLVLPEMVERNSGHIINIGSIAGTYPYPGGNVYGGTKAFVKQFSL
                     NLRADLAGTKVRVSNVEPGLCGGTEFSNVRFKGDDEKAAKVYENVQFVRPEDVANIVL
                     WLNQQPEHVNINRVEVMPVAQTSSALNVARNI"
     gene            12800..13990
                     /gene="trpB"
                     /locus_tag="PWX02_001055"
     CDS             12800..13990
                     /gene="trpB"
                     /locus_tag="PWX02_001055"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDL1729081.1"
                     /translation="MSDTLLNPYFGEFGGMYVPEILVPVLKELEQAFVDAQQDPTFQA
                     EFADLLKNYAGRPTALTLCRNLTKGSKTKLYLKREDLLHGGAHKTNQVLGQILLAKRM
                     GKTRIIAETGAGQHGVATALACAMLGMPCVVYMGAKDVERQSPNVFRMRLMGAEVIPV
                     QKGSCSLKDACCEAMRDWAANYDTTHYLIGTAAGPHPFPTMVREFQKMIGEETKRQIL
                     AKEDRLPDAVIAAVGGGSNAIGMFADFIEEQSVKLIGVEPAGKGIETGEHGAPLKHGT
                     TGIYFGMKSPIMQDKDGQIEESYSISAGLDFPSVGPQHAYLNATGRAEYVSITDAEAL
                     EAFQALAKNEGIIPALESSHALAYALKMIKQNPEKEQLLVVNLSGRGDKDIFTVDKIL
                     NGGE"
     gene            13992..>14746
                     /gene="trpA"
                     /locus_tag="PWX02_001056"
     CDS             13992..>14746
                     /gene="trpA"
                     /locus_tag="PWX02_001056"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDL1729082.1"
                     /translation="MSRFDTLFTALKAKNEGAFVPFVTLCDPGFERSFEIIDTMIKNG
                     ADALELGFPFSDPLLDGPVIQAANKRALDGGYSTDSCFELIAKIRAKYPDIPIGLLLC
                     ANLIFVPIAEVFFQRCQQSGVDAVLIADLPVFAAQEFVPTAEKYGIQPVFICPPNADQ
                     NTIERIAQLTQGYTYLVSRAGVTSAENQAHASNLDNLVESLKQTGAAPILQGFGIAKP
                     EQVKEALRLGADGAISGSAIVKIIERNLSNQAN"
BASE COUNT         4362 a         2612 c         3202 g         4570 t
ORIGIN      
        1 gcttttaaat actaaaagcc atctaaaatt gaagtgttaa agtatagaaa tagtagaaaa
       61 atgtagttgt tatgggaatt aagaaaggta tctacgaact agtttcttaa gatcgcttaa
      121 atggctagca ttccatccat gagctattgc ccagctagct atttgttctt catccgcttt
      181 atatcccttt tgagatagct ctttgaagat ttgttcagca tgctttttat ctgatgggtg
      241 cgatagccca gtagatagat tgataatagc agttaaatta gtcaatgact gtacaatttc
      301 ttccggtatt tgtggaatat taagagaagt ttcccctttc atgataataa gtccatgatt
      361 atgccaagta ttctcccaat gattagcttc atctggcaac caggatacga atataatgtc
      421 atcagcagaa gagtttgagt cgatcaaatg catatcatga gatgtacagt aaacagccaa
      481 gattgtacca gatgagtttg aagaattaat tcttgatgga agtagcaaat ctaaggaacc
      541 tatagattca cctttagtta attttttagc tgatcctgct cctaatattt gatcaattat
      601 tttcccaaga ttagtacttt caaagtcaga ctttctggga aaaacaatat gaatgttaga
      661 attgttattt ttccgagata tttcaacaga atgttgaatt gcttgaatta tagtttgatc
      721 atctggacca taatttttaa ataaaaatct acgtttcata taagctcctt tttattacaa
      781 tgcattagat ttagtatatt gaatatccat atataagcat agaataattt ataaaaccta
      841 tctaggttta ttgtcaaaat taaggaaatt ccaaccatgc gcaaactcca aaacaccctc
      901 tatatcacca gccaaggtag ttacttgcac aaggaacgtg aaacactagt tgtggagcaa
      961 gatcgaaaaa aagtggcaca attaccgata cattccatcg ggcatatttt ctgttttggg
     1021 aatgtattgg tatcgccatt tttaatggga ttttgtggcg aaaataacgt aaatttggcg
     1081 ttttttaccg aaaatgggcg ttatcttggg cggttgcagg ggcggcaaag tggtaatgta
     1141 ttgttacgcc gagcccagta taaaaagtcg gagaccaacc ctgttcctgt ggctcgcaac
     1201 atcattgcag ccaaaatcca agcatctaag cgagtgttac aacggcaaat tcgtaatcac
     1261 ggcgaaaatg cggatattca attagcaatt accgcattaa attacagctt gcaacagttg
     1321 aaaatagcgg acaatttgaa tttgattcgt ggtattgaag gcgatgctgc ggctcgttat
     1381 tttgggatgt tttctcatct tatcaaagaa aatagcggtt tccactttga cggacgtaat
     1441 cgccgcccac ctcgtgacgg tgtgaatgct ttgctctcat ttctttatag tattttaggg
     1501 aaagatatta gcggggcatt gcaaggcgtg gggcttgacc cgcaaattgg ctttttgcat
     1561 gcagatcgtc cggggcgaga tagcttagct caagatattt tggaggaatt tcgagcgtgg
     1621 tgggtggaca gaatggtgct gtcgctgatc aatcgagggc aggttaagcc tgatgatttt
     1681 gtgacggaag ccagtggtgc ggtaacgctc aagcctgaag caagaaaatt actatttcaa
     1741 accttgcaag cgaaaaaaca agaaaaaatc attcacccat ttttagagga agaagtggaa
     1801 atcggcttgt taccttatat ccaagcgatg ttattggctc gtcatttgcg tggcgatttg
     1861 gcagaatatc cgccgttttt aatgcgttaa aatctgcaaa attttgttga aatttgaccg
     1921 cttgtttatg ttttattcat tgaggaatta gcaactatgc tgatgttgat tacctacgac
     1981 atttcatttg atgaccccga agggcaggct cgcctacgcc gtattgctaa gcattgtttg
     2041 gattatggtg tgcgggcgca atattctgtg tttgagtgtg atgttactcc tgctcagtgg
     2101 gtaacattga aaaataaatt attggcaact tacaacccag actgtgatag tttgcgcttt
     2161 taccatttag gcagtaaatg gaggaataaa gttgagcacc atggcgcaaa accttcagtg
     2221 gatattttta aagatacgct gattttataa atcgctagcc tgtagttctc ataaaaatgt
     2281 agcaaggtta gcgaatctat attgttcttt aataatcaag gaattagaaa aaataaggca
     2341 cagtatattg gcgatatgct atactttatc cactctctac aaaacgttag cgaataaagg
     2401 gctaaaaagc ctgatatttc aatacgttat cttgtaaggg cagccaccta cgcgtggctg
     2461 tgaattgaaa caccacctcc tccgagatgg taggcaaggg gattagcagc cacctacgcg
     2521 tggctgtgaa ttgaaacacc cgcttgcggt aaagctctag gctctcaccc tgcagccacc
     2581 tacgcgtggc tgtgaattga aactcttaaa tcgctatcgc tctcttcatc tgcgccaagc
     2641 agccacctac gcgtggctgt gaattgaaac caatcaaggc ggccatttcg ggggtaaatt
     2701 cgtgcagcca cctacgcgtg gctgtgaatt gaaacaggga taagctcttc gccctcgctg
     2761 cattgcatta gcagccacct acgcgtggct gtgaattgaa acgcttacat ctagacagac
     2821 gagcaagtgc tgaaacgcag ccacctacgc gtggctgtga attgaaacac tgacatcggc
     2881 gtaaatgcgt ggaagatttt cgagcagcca cctacgcgtg gctgtgaatt gaaactccgt
     2941 gtttgtggtt atgttgatgt acaggcaatt gcagccacct acgcgtggct gtgtattgaa
     3001 acggtgtacc cacaaatcct atgttttcgc cattggctgc taccttcgca tcaatatata
     3061 ttgaaatatc aatggcaaat ttgtatgttg ttaattagac tgattgcatc tgctatgtat
     3121 catagtaatg acgaaaatat tctattttgc aaaaaataat cagaaattac ccgcttgttc
     3181 gaactgagtt tttgttttct aagatgtgaa atgatacact ttagatagtt ttaaaatata
     3241 gagacagtaa gatgagtagt acggaattat tagtgaatgt taccccaagc gaaacaaggg
     3301 tagcgttggt agaaaacgga atattgaaag aattacatat tgagcgggaa gctaaacgcg
     3361 ggattgttgg taatatttat aagggaaggg tgacaagggt attaccaggt atgcaatctg
     3421 cttttgtgga tatcggcttg gagaaggctg cttttttgca cgcttctgac atcgtttcgc
     3481 atacggaatg tgttgatgaa aatgaaaaga agcaatttct ggttaaagat atttctgaat
     3541 tggtgcgtga agggcaagat atagttgtac aagtagtgaa agatccaatt ggtactaaag
     3601 gagctaggct cactactgat attacgttac catctcgtta tttagtgttt atgccagaaa
     3661 atagccatgt gggagtatct cagcggattg aaagtgaaga agagcgtgct cgtttaaaaa
     3721 gcttggttga gccttattgt gatgaacttg ggggctttat tgtacgtacc gctgcagaag
     3781 aggcaactga agaaagttta aagcaagatg ttgaattttt aaaacggttg tggcgtaaag
     3841 tattagaacg aaaagggaaa tattccgcaa aatcaatgct ttatggagag cttgcgcttg
     3901 ctcaaagggt actgcgtgat tttattggta cacatatcaa ttcggttaga atcgattcaa
     3961 agctaactta tgagcaagtg cgagaatttt tagctgagtt tatgccagaa ttaacggatt
     4021 gtgtcaccct ctataccggt cagcaaactt tatttgatgc ctatggtgta gaaaaggcaa
     4081 tccaaaaggc attggataaa cgggttgatt taaaatcggg gggctattta attattgagc
     4141 aaactgaggc gatgacgact attgatatta atactggtgc ttttgtcgga catcgtaacc
     4201 ttgcacatac gattttcaac acaaatattg aagcaataca ggcaattgca caccagctac
     4261 agttgcgtaa tttaggtggt attatcatta ttgattttat tgatatgcaa gaggaagaac
     4321 atcgtgagcg cgttttacaa tcgttacaag aggcattaaa aggcgatcgt gttaaaacca
     4381 atgtgaatgg ctttactcag cttggcttgg tggagatgac cagaaaacgt acaagggaga
     4441 gtctagagcg aattctttgt tgcgattgcc aagcttgcca agggcgtggc acagtgaaaa
     4501 ctgtagagac ggtttgctat gaaattatgc gtgaaattgt gcgtgtacat catttgttta
     4561 agacagaaaa tattcacgtt tatgcagcta aagatgttgc tgaatattta attaacgagg
     4621 aaagccacgc attattggct gagcttcagg cgtttatcgg taaaaaaatt gagattaaac
     4681 ttgagccata ttatcatcaa gagcagtttg atgtggtcgt gatgtaatct attacttggt
     4741 tgaggctaaa tagttagcct caatatcgtt agttacaaaa aatttagaaa atctgaccgc
     4801 ttgtttccta tccaaattcc tcaaaataac gtataatcgt ccagttttat ttatcctttt
     4861 ttgaaatcta aggtaattca atgtctgaag tagaaaatca agaattagat ctaaacggtg
     4921 aaatgttagc ccgccgtgaa aaattaaaca aaatccgtga gcagggtaac gcatttccaa
     4981 atacattccg tcgtgatgcg ttagccaaag atctgcacgc tcaatatgat gcagtggaag
     5041 gtgaaacctt aaaagaacag aatattcaag tgaaagttgc cggtcgtatt atgttaaaac
     5101 gagtaatggg taaggcatct ttctttacca ttcaagatgt gagcggtgcg attcagcttt
     5161 atgtagctcg tgataactta agtgacagcg tttacgaaga aaaagtcagc ctttgggatt
     5221 taggcgacat tgtgggcgtc gaaggtgcgt tatttaaaac caaaaccggt gaattaacgg
     5281 ttcgttgctc tgaagttcaa cttttaacca agtctttacg cccgttgccg gataaacatc
     5341 acggtttaac ggatttagaa actcgttatc gtcagcgtta tttagacttg atttctaatg
     5401 aagaatctcg ccgtactttt atgatccgct cgcaagtagt ttcaggcatt cgcaaattct
     5461 tcttagaaag agactttatt gaagttgaaa cgccaatgtt gcaagtgatc ccgggcggtg
     5521 cggcagcgaa accgtttatc actcatcata atgcgttaga tgtggatatg tatctgcgta
     5581 ttgctccaga gctttactta aaacgtttag ttgtgggtgg ttttgagcga gtatttgagc
     5641 ttaaccgtaa cttccgtaac gaaggggttt cagtacgcca caatcctgaa tttacaatga
     5701 ttgagtacta ccaagcctat gcggattatc acgatttaat ggataatacc gaagaactgt
     5761 tacgccaatt agccttagat attcttggca caactgacgt accttatggt gattatgtat
     5821 tcgatttcgg taaaccgttt gagcgtatta cgatgcacga tgcgattgtg aaatatggta
     5881 acggcattca acgtgaagat ttagacagct tcgagaaaag tgtggaaatt gcgaaaggct
     5941 taggcattga aattcaaaaa tcttggggtt taggctcggt agtcaatgcg atttttgaag
     6001 aagtggcaga acatcaatta attcagccaa ctttcttgat ggctcacccg gcagaaattt
     6061 caccattagc tcgtcgtaat gatgaaaacc cagaggtaac tgatcgtttt gagttattta
     6121 tcggtggtcg tgaaattggt aatggtttct cagaattaaa cgatgcagaa gaccaagcag
     6181 agcgttttga tgctcaagtt gcggcgaaag atgccggtga tgatgaagca atgtttaaag
     6241 atgaagactt tatcgtggca ttggaacacg gtctgccgcc aacagcgggt gaaggtttag
     6301 gaattgaccg cttagcaatg attttcgcta acgccccatc aatccgtgat gtaattttat
     6361 tcccagcgat gcgtcagaag taatcagaaa tagaacaaaa gcacacttcg gtgtgctttt
     6421 ttgtattaca agcggtcaaa tttataataa aaaatgcaat ttattttgct aatccggctt
     6481 taatcatcgg ttcaaaaaag tcataatcaa ccaaaaaggc atcgtgtccg aaatcggagt
     6541 gaaactcgtg gaactccaaa tttacgcctg cctgttctaa tttttgtttg ctcttataca
     6601 cgtcaacttg cttgaatagt tggtcgcttg ttaccgctac aaaagtgtag ttggctttaa
     6661 tacgttttag ggcggtttct tcattttcat agccaagagc cgggtcgtat aaatctaatg
     6721 ctcggagtaa acggaggtaa gtgttggcat cgaaacgacc taaaaatttc gtgccttgat
     6781 agcttaaata ggattccact tggaaatgat cgccccaaat ttgtccttgc tgtttggttt
     6841 ctcgtccaaa ggctttagca agttgaatat cagtacggta agtcagcata ccgagcatac
     6901 gggcgatttt taagccgtta tcaggtgcaa tgccatcata atagttccca ccgttaaagt
     6961 gtggatcgtt aataatagct tggcgcataa cgtggttaaa accgatagcc tcggcactta
     7021 gggtaagtga cgaacaaagg tttataatgt tgcgaacttt atcagggtaa ctaatacccc
     7081 actgtgttgc ttgcattccg ccgaaagatc cccctaccac tgcgtgaagt tgagggattt
     7141 ctaaatagtc caataatgct ttttgtacat taacaatatc ttgcactgta ataattggaa
     7201 acaaactacc gtaaggttta ttggttttag ggttaatcga agctgggccg gttgtgcctt
     7261 tacaaccgcc taatacatta ctgcaaataa agaaatattt atcggtatca aacgcaagcc
     7321 ctttgccgat gaaatcttgc caccagcctt tctcggttgg tgagtcatag taaggcttag
     7381 catcaccggt aagggcgtgg caaagcaaga ccgcattgct tttctctgca tttaatctgc
     7441 cgtaagtctg gtaagccact gtgatagggg acagttcgcc tcctaatgtg agtggtaaag
     7501 gtacgtcctc aaaaagggta atctgctgag ccataaaaat cctgtttgca aaaaattctg
     7561 cttttttgac cgcttgttgt aatgaccgtt aaattagcgg tgttcatttc aattgtcaag
     7621 aatttttaga cgtctaaacg tctagatttt taaacggcta aaatcaaaat tttattgctg
     7681 agaagatatt tattacttaa caaataacat gaaatagaat aaatttattg aacgattaga
     7741 ctattttgtt tgtctctaat ttacccatct taaatagggt agcaaaagta aaggatctca
     7801 tttgaaccaa acgcgtgata ggttagaaaa attgccactt ttccataaaa tgcagcagtt
     7861 tattattaag gcattgaaaa taattgcaat tttcccctta ttgggaatag ctatattatt
     7921 attggttgat tcacttacaa gttatattgt gagggataaa atttatacag atagtaaaca
     7981 gattccttac cgggagtatg cagttgtact cggtacggca aaattttact ctaaagatgt
     8041 gattaaccaa tattataaat atcgtttgga agccgctaag caattagtcc aagaaggaaa
     8101 ggtcaatcag cttttgttaa gtggagataa taaaacgccg tactataatg agccaaaaac
     8161 aatgacaagt gatctactta aaatggggat tgcgacttca aaaattaagc aggattatgc
     8221 ggggtatacc acttttgatt caataattag ggctaaaaaa gtatataaat taccgccatt
     8281 tacgattatc tcccaaaaat tccactgtga aagggcattg tttattgcaa aatttaggga
     8341 tattgatgca atttgttatg ccgctacata tccagagggg gcttatcagg tacgaatgcg
     8401 ggaaatttta gccagatcgg caatggtttt tagtttgttg ataggtaaag agcctgaatc
     8461 attggaagat atccgttcta aatgagatag ccggtcagat aatgttgacc ggctattttg
     8521 cttataaaat aatggttttg tttttatata caaatacttt gtcagcaagc actaattcta
     8581 aggcttggct aagtacggtt ttctcgacat cacgtccggc acgcatcata gcctctgcgg
     8641 tgtaggtgtg atcaacatta atcacatttt gcataataat cgggccttca tctaattcat
     8701 cattcacaaa atgggcggtt gcaccgataa ttttcacacc acgttcgtat gcacgatgat
     8761 aaggttttgc accaatgaaa gcgggtaaga acgagtggtg aatattgaca actcggtttg
     8821 gataacgagc gacaaattcc ggattcagca cccgcatata tttagccaac acaatataat
     8881 caggtgaata ttcatcaatt ttttccgcta ataatttatc gtgttccact cgggttaaat
     8941 tttcgtggct gactaaatgg aatggaatat cgaaacgttc aactaaactt tttaatgttt
     9001 cgtggtttcc aatcacggca gcaatttcta catctaatcc accatagtaa tttttcatca
     9061 gtaagtcacc taaacaatgt gcttctttgg taactaaaat aacgatacgt tttctttgtt
     9121 tagggagtaa tttatagctt gagccttcag gtaaggtaaa ttttaaatca gtgagtaagg
     9181 tttcatcatt aaaaattccg tttaattcgg tacgcataaa aaaacgttta gtttcattct
     9241 caacaaattc agaattttta ataatattta attggtgctt atagcaaata ttggtaattt
     9301 tagcgacaag tcctgtatca tcaggacatt cggttaataa tattttgtgt tgttgtagca
     9361 tttcatttcc tattataaaa ataagagaac tcaattgagt tctctttaaa ctaaaaatag
     9421 ttatagccaa ttaaatagca aaatcttcta attttttacc actatctaaa gcatcttgta
     9481 atgcctttgg tgttctgcct tgacctgtcc aagttttctc attgccgtta gcatcaacaa
     9541 atttatattt agccgggcga ggtgatagtg ctttacgtgc tttgcgggtt ttgattactt
     9601 tatctgctaa taattcagct aattcatcaa tagataaccc atcttgtttt agcatttctc
     9661 tatatttatt taagctttct aattgtttta gtttttctaa ttcttcctgt ttcatttctt
     9721 cttctttttc agcaataatt atatctaatt tctcagaaat tgattttaat tgctctaatg
     9781 ctaaatcttt agccaaagcc cttaagctgc ggatattcaa aagacttttt aaagctgaca
     9841 ttttactctc ctaaattaat gttttatata agtagctttt atgctccttt tatcatatct
     9901 caaaaggcat tttaggtaaa taagcataaa taaaaaaata gatactttat atctaaagat
     9961 attcgtgcta tcttaaacac tgtcctacaa attgtagagg tgcgaattca ataagtattt
    10021 cttctgagtg gaaaacgatg aaggggaagg aaaggtgaat ttgccgaaat caattaagcg
    10081 tcatcttaat tggttggggt cgttgtcgaa agaaacgaca ctgtcgtagt aaatgctacg
    10141 gagtgctact gttagggtgg gtttcagata tttcacattt gccttccttt catatttatt
    10201 tttataaata tcccagtaga tctcttgttt ggctattctt atttatacaa attatacaca
    10261 gtcattttag aggttttatg aatctagttg attattctac gtcactttgg tcggtattgc
    10321 cggcactgat tgcgctaata ttggcaattg tgacacgcaa ggtgattatt tcacttagca
    10381 tcggtattct ctttggtgca tttatgctta ctaatagcaa ttttcttgat gcacttactt
    10441 atattaaaaa tagcgttgtt tctttagttt ataaaggggc tgaagaagga ttaaattcaa
    10501 ataatgttaa cattatttta tttttgcttt tattaggggt attaacgtca ttattaagta
    10561 tttcaggtag taatcaggcg tttgcgaatt gggcgcagaa aaaggtaaaa ggcaaacgag
    10621 gcgcaaaatt aatggcggca ggtttggttt tctttacctt tattgatgac tatttccaca
    10681 gtttagcagt aggtgcgatt gcccgccctg ttacggataa attccgtgtt tctcgtgcta
    10741 aattagctta tattttagat tctaccgccg ctccaatgtg cgtgttaatg ccaatttcaa
    10801 gctggggggc ttatattatt accttaatag ctggcttatt ggctacgcat tccatcacag
    10861 gctattcgcc actaggcgca tttatgacaa tgagcgcaat gaacttttac gccatttttg
    10921 cgatgattat ggtgttcttt gtggcatatt tctcatttga tattggttcg atggcgcgtt
    10981 ttgaacgtca agcagcgcat ttagcagaat tagatgatga gcaaattgaa acgaaaggac
    11041 acgtgcgcga tttgattttg ccaattattg cacttattgt tgctacagtc tcaatgatga
    11101 tgaaaacagg tgcagatgca ctggccgaat caggtgcttc attttctata ctaggtgctt
    11161 ttgaaaatac tacggtaggc atttcattag ttgttggtgg tgtggcaggg cttatagttg
    11221 caactatttg tatcattaca agcggtaata tttcttttaa aaattacgtc cgtgcttatg
    11281 gcttaggtgt gaagatgatg tctggtgcaa tcactattct cttctttgcg tggacaataa
    11341 acggcgtagt aagtgatatg caaacaggga aatatctatc aggattggtt gcagaaagca
    11401 ttggagcaga gtttttacct gcaattctat ttgtgttagc cgcagcaatg gcgttttcaa
    11461 caggaactag ctggggaaca tttggtatta tgttgccaat tggagcctca atggcagtgc
    11521 attcagatcc agcattaatt attccttgtt tatctgcagt aatggctggt gcggtttgtg
    11581 gtgatcactg ttctccagta tctgatacca ctattctttc ttcaacaggc gcacaatgta
    11641 atcatatgga tcatgtcatt tctcaattac cttatgcttt attagttgca acagcttcta
    11701 ttgttggtta cttagtggtt ggctttaccc aatcagcggt attgggattt attgctacag
    11761 ctattgttat ggcaatactc attttgcttt ttaaagaaaa aaagcattaa tttccgaaaa
    11821 tgtgaaggga atcacagaat gagatttatt gatgagataa aatagcactc aagatatgga
    11881 tatcgaatgt tgtgttatat aaatcttaaa ataagatcct cctagcatta gcgtaccttt
    11941 tggtacgctt ttttctttta tggcaatatt atttcttgca attttaattt ttttgtacta
    12001 ctttaatagt acattttaaa tttgagggtg aaaagatgaa aatagcatta gtcacaggag
    12061 taactgctgg atttgggttg gcaatttgta aattattgct tcaatcgggt tataaagtaa
    12121 ttggcacggg cagacgggca gagcgtttag aagaactgca tttacaattt ggcgataaat
    12181 ttctaccgct tgcatttgat attcaacagc ccgatgaggt ggaaaaagca ctgaatagcc
    12241 gccctaaagc gtggcaacaa attgatttat tagtaaacaa tgcaggcctt gcactcggct
    12301 tagaaccggc acagaaagca gatttgaacg attggtatca aatgatcgat accaatatta
    12361 aaggattggt aacgatgaca cgcttagttt tgcctgaaat ggtggagcgg aacagtgggc
    12421 atattattaa tatcggctcg attgccggca cttaccctta cccgggtggt aatgtatatg
    12481 gtggtactaa agccttcgtg aaacagttta gcctaaattt gcgggcagat ttagccggca
    12541 ctaaagtgcg tgtttccaat gttgagccgg ggctatgtgg cggtacggaa ttttcaaatg
    12601 tacgctttaa aggcgatgat gaaaaagcgg ctaaagttta tgaaaacgtg cagtttgtgc
    12661 gcccggaaga tgtggcaaat attgtgcttt ggctgaatca gcaacctgaa cacgttaata
    12721 ttaatcgggt tgaagtgatg ccggtggctc aaacttcttc tgccttaaat gtagcgagaa
    12781 atatttaaaa ggaaatatta tgtcagacac acttcttaat ccttattttg gtgaatttgg
    12841 tggtatgtat gtgccggaaa ttttagtgcc ggtgctaaaa gaattagaac aagcctttgt
    12901 agatgctcag caagacccaa cattccaagc tgaatttgcc gatttactca aaaactatgc
    12961 cggtcgcccg actgctttaa ccctttgtcg taatttaacc aaaggcagta aaaccaaact
    13021 ctatttaaaa cgtgaagatc ttttacacgg tggggcacat aaaaccaacc aagtgctagg
    13081 gcaaatctta ctcgctaaac gaatgggtaa aacccgcatt attgccgaaa caggagcagg
    13141 acagcacggt gtggcaaccg cattagcgtg tgcgatgtta ggaatgcctt gcgtggtgta
    13201 tatgggcgca aaagatgtag aacgccaatc gccaaatgta ttccgtatgc gtttaatggg
    13261 agcggaggtg attccggtac aaaaaggctc ttgctcgctc aaagatgcct gttgcgaggc
    13321 aatgcgggat tgggcggcaa attatgatac tacccattac ctaatcggca ctgcagccgg
    13381 cccgcacccg ttcccgacaa tggtgcgtga atttcagaaa atgattggcg aagagaccaa
    13441 acgtcaaatt ttagcaaaag aagaccgctt gcctgatgcg gtgattgctg ccgtaggcgg
    13501 tggatcaaat gctatcggta tgtttgcaga ttttatcgaa gagcaatccg tcaaattgat
    13561 cggtgttgag ccggcaggaa aaggaattga aaccggtgaa cacggtgcac cgctcaaaca
    13621 cggcacaacg gggatctatt tcggtatgaa atcgccgatt atgcaggata aagacgggca
    13681 aattgaagag tcttactcca tttcagccgg cttagatttc ccttctgtcg ggccgcaaca
    13741 cgcttattta aatgcgacag ggcgagctga atatgtatcg attaccgatg cagaggcttt
    13801 agaggcattc caagcattag ccaaaaatga ggggattatt ccggcgttag aatcttccca
    13861 cgccttagcc tatgcgttaa aaatgattaa gcagaaccct gaaaaagagc agttattagt
    13921 ggtgaattta tcgggtcgag gcgataaaga cattttcacc gtggataaaa ttttaaatgg
    13981 aggagagtaa aatgagccgc tttgatacgc tatttactgc attaaaagca aaaaacgaag
    14041 gggcttttgt gccgtttgtg acattatgcg atcctggttt tgaacgcagt tttgagatta
    14101 tcgacacgat gattaaaaac ggggctgatg ctttagagct aggctttccg ttttctgacc
    14161 ccctcttaga cgggccggtg attcaagcag cgaataaacg agctttagat ggtggctata
    14221 gcactgatag ctgttttgag ctgattgcca aaattcgagc taaatatcca gacattccta
    14281 tcggtttact actgtgcgcc aatttaattt ttgtgccaat cgcagaagta tttttccaac
    14341 gttgccaaca aagtggagtt gatgcggtgt tgattgctga tttgcctgtt ttcgctgccc
    14401 aagagtttgt gccaaccgct gaaaaatatg gaattcagcc ggtgtttatt tgcccgccga
    14461 atgctgatca aaacaccatt gaacggattg ctcaattaac ccaaggctac acttatttag
    14521 tttcccgtgc cggtgtaact agtgctgaaa accaagctca cgcaagtaat ttagataatt
    14581 tagtggaaag tttgaaacaa accggagctg cacctatttt acaaggtttt ggcatagcca
    14641 aacctgaaca ggtgaaagag gcgttaaggc tcggagcaga cggggcgatt tcaggctcag
    14701 cgattgtgaa aattattgaa cgtaatttat ctaaccaagc taattt
//
LOCUS       DANJWC010000047        14228 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.047, whole genome shotgun sequence.
ACCESSION   DANJWC010000047 DANJWC010000000
VERSION     DANJWC010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14228
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.047"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..860)
                     /locus_tag="PWX02_001057"
     CDS             complement(<1..860)
                     /locus_tag="PWX02_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDL1729083.1"
                     /translation="MKFTDIFIKRPVLAVCISLLITILGLQAIEKLQVREYPEMTVSI
                     VTVSVNYSGADARLMQTLVTSQLEEAVAQADNIDYMTSSSSPSTTTVTAKMKLNTDPN
                     AALSDISAKVNAVRSNLPSGIDDPSIRVSTGSNDALMYIRFVSDELNTSQVTDYINRV
                     VKPQFFTVNGVSSVDVYGSVFGLRIWLDPDKMASNNLSGAAVLSALSANNLQTAAGNS
                     NGYYTVYRNKVLSTTPSVTELEEVTVSTTKDGKTIKLKDIATVELDKYQDAARATVGG
                     KDAVVLAVSVA"
     gene            complement(874..2079)
                     /locus_tag="PWX02_001058"
     CDS             complement(874..2079)
                     /locus_tag="PWX02_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDL1729084.1"
                     /translation="MTTETQKLGKGRKFLIVLTLLIVLIVFAAIVGLQKIGAMKKAEA
                     AANMPEAVSEVTAMQVTTQEWTPTISAVGYIRPNQGAMLSAETSGVVSRVLVTSGQRV
                     KKGDLLVEFDSSVEIANLRASEAQLPNAKANLERYRNLIASNSASKAEFDNAQSTYNQ
                     LLANIEALRATIKRRQIYAPFSGLAGIVNVNVGQYITMGTEIVRVEDQSSMKVRFTLP
                     QTNLEQISIGQKVTAVIDALPAQTFPAKIVAIDPAVDRLTGLINVEAVIEEGQEKLLS
                     GMFARLNVALPTETGQIVVPQISIAYTMYGETVYVLQPLSDEDKEMVNKMAVQNPSLD
                     VNKMYRAKQMEVKTADRKGNYSQLSKGVKAGDLIVTGGLQRLSNNALVKVSDTEAVGI
                     TTPAKNSKL"
     gene            complement(2110..2682)
                     /locus_tag="PWX02_001059"
     CDS             complement(2110..2682)
                     /locus_tag="PWX02_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL1729085.1"
                     /translation="MSRRSEPKEEMVNRILSATESLIVQEGLQNLSMRSIAKEAGIAS
                     GTLYLYFKTKDDLLHSLAAQLLECYNGYMHFEFKPEMALFEQYKALVNKKWQVLIERL
                     DLVKQFQAVIGIDELIRETIADETSFWNKLILEGKKQRIIADLPNELLYGLSIGTLVD
                     IQYLQNLNQENQFDGYLDEIILRTWKAITF"
     gene            2921..3142
                     /locus_tag="PWX02_001060"
     CDS             2921..3142
                     /locus_tag="PWX02_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1414 domain-containing protein"
                     /protein_id="HDL1729086.1"
                     /translation="MATKSKYHTKQFEQLLADLIATIEKHKAPVDLSLMALGNMITNI
                     LIENIQSPAQREALADAFADALKQSLKTK"
     gene            3147..4880
                     /locus_tag="PWX02_001061"
     CDS             3147..4880
                     /locus_tag="PWX02_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3413 domain-containing protein"
                     /protein_id="HDL1729087.1"
                     /translation="MFARLRSLLPTNSRQYREETSQKITWGHWFALFNIVLALLISSR
                     YAFNADWPNTLVGKLYFFVSLFGHFSFVVFAGFLLILFPLSFIVKNHRTYRGLSVILA
                     TIGQTVLLVDTEVFKQFYLHLSPLVWDLLVNPEEGELTRKWQLLFVPMPLLLLAEMLY
                     SRWCWQKLRSFSRQKWGKYVALFFVISFTATHLIYAWADMNAYRPITAQRANYPLSYP
                     MTARNFLEKHGFIDRVEFNQEIEESGRLDTFYLNYPKSPLIATKTPSTNILLINLSGL
                     SKQIINAETMPKLTEISQQSHRFMQHYSSGDSASAGALGLFYSLSGKYLDSVLADKTV
                     SPLIEIARKSDYQLGLFSHNGFADPIYHQAIFSGILLPTAHSNVGAIGQWKNWISSRS
                     LENPFFSYLDLRMPFAHTAQEQAKLLDWQFAEIWQHLTQNALLANTLVIITADISNEV
                     GAENSFDKQHIQVPMMFYWQGESKEYHFLSSHLDIMPTLLSQFFGVQNPLNDYAQGMD
                     LSTQNQRLWVSSSNHKWNVAIMPNGDQYQIDRKGNFKHFNSNGEHEKDARPPLALFLQ
                     MIQQSNQFIEK"
     gene            complement(4947..5595)
                     /locus_tag="PWX02_001062"
                     /pseudo
     CDS             complement(4947..5595)
                     /locus_tag="PWX02_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484232.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(5592..5972)
                     /locus_tag="PWX02_001063"
     CDS             complement(5592..5972)
                     /locus_tag="PWX02_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1729088.1"
                     /translation="METLAIFLFSEIWKFDFVFTFIFKILKINVFFSDLINMKLARKA
                     QRRLSFLRVFSLAFAAFIVNTTEFIPIALLSDIASSFQMEPSSAGIMMTVYAWVVFLL
                     SLPLMLLTANMERKRLLLMLFMGE"
     gene            complement(6014..10474)
                     /gene="mukB"
                     /locus_tag="PWX02_001064"
     CDS             complement(6014..10474)
                     /gene="mukB"
                     /locus_tag="PWX02_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HDL1729089.1"
                     /translation="MIDTTELQNNPQIAPLARPNDVQRGKFLSLTLINWNGFFARTFD
                     LDDLVTTLSGGNGAGKSTTMAGFVTVLIPDLTLLNFRNTTEAGSTAGSRDKGLYGKLK
                     AGVCYAVLESFNSRGQRIITGVRLQQIVGRDKKVDIRSFSLQNIPQEQSIISLLTEQV
                     GNKARVLALNELKDQFENSEVQFKQYHSITDYHSFMFDLGVIPKRLRSAADRSKFYKL
                     IEASLYGGISSVITKSLRDYLLPENTGVRQAFQDMESALRENRMTLEAIKVTQSDRDM
                     FKRLITESTNYVSADYMRNANERRGNVQQALEQRKEWYAAKSKILLEQQRFVEFSRES
                     ADIAEAEQALEADYNSANDHLNLVMNALRHQEKIERYQDEVEELNIKLEEQQEALEEI
                     AEIAENAQARADEADDYVEELRSQMADYQQALDAQQTRALQYQQAVNALEKAKQLTGL
                     VNLDLNNIEDYHAEFVAQAEDLTDQVFELEQRLSVSDMAKTQFEKAFESVCKISGEID
                     RLQAWEEARALLSAFPEQKMQAQQAVSLRQKLNDLEQRLQQQQNAQRLVAEFNQKSQQ
                     QLNSAEELDAYFEEQQARLEDLEAELSDFVEQRSTQRQQREQLNQQYQQLAKNAPAWH
                     TAQSALTRLEEQCGEKFEASQSVMQFMQNMLSKEREATLARDELARKEQLLDEQINRL
                     SQPDGSEDARLNQLAERFGGVLLSELYDDVSIEDAPYFSALYGEARHAIVVRDLEAVK
                     TQLEKLDDCPSDLYLIEGDPSAFDDAVFSAEELGEGVVVKVSDRQWRYSKFPEVPLFG
                     RAAREKHLATLKAERDETAEKHAERAFDVQKCQRLHQHLSQFIGTHLNLAFQENPEML
                     MQEIAAERAEIERELAQSAGNEQQLRHQLESVKAQLQMLNKILPLANLLADETLEARA
                     EECREQLELAEEDEMFIRQFGATLSQLEPIAAALKSDPALFEQLEADYRRSVAEQKVL
                     QQKVFSLADVMNRRLHFSYQETVGTEGSALTEQLRQRLENAQREREQARDQLRQAQAQ
                     LSQYNQVLTGLRSSYEAKNQMLQELIREIDDLGVRGDSGAEERARFRRDELQQRLSQQ
                     RSRKGYLDKQIAVIEAEMDNLNRTLRKAERDYHTQRELVVQAKASWCIVQRLSRNSDV
                     EKRLNRRELAYQSAEELRSISDKALGALRTAVADNEYLRDSLRASEDSRKPENKVAFF
                     IAVYQHLRERIRQDIIKTDDPIDAIEQMEIELSRLTNELTGREKKLAISAESVANILR
                     KTIQREQQRILQLNQGLQNIAFGQVKGVRLVVNIRDTHAILLNALSNNREEHKDLFDS
                     QKLSFSEALAMLYKRVNPHIEMGQRTPQTIGEELLDYRNYLDLEVETFRGADGWMRAE
                     SSALSTGEAIGTGMSILLMVVQSWEEEARRMRAKDILPARLLFLDEAARLDATSINTL
                     FELCERLDMQLLIAAPENISPERGTTYKLVRKITNNQEYVHVVGLKGFAK"
     gene            complement(10650..11366)
                     /gene="mukE"
                     /locus_tag="PWX02_001065"
     CDS             complement(10650..11366)
                     /gene="mukE"
                     /locus_tag="PWX02_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HDL1729090.1"
                     /translation="MTENIQDFISPKLALAIANPIFPELDSQLRAGRHINTEQLDQHV
                     FLMDFQAELESFYRRYHVELIRAPEGFFYLRPKASTLIARSAMSEMEMLVGKVLCYLY
                     LSPERLAQQGIFSQDDVYEELLSLADESKLLKAVNPRSTGSDLDRAKLAEKVGGALRR
                     LARIGIITRVGEQNSKKFVISESVFRFGADVRTGDDPREAQLRLIRDGEATTPALLNE
                     QAVEIDENIADEAPFDGENE"
     gene            complement(11415..12758)
                     /gene="mukF"
                     /locus_tag="PWX02_001066"
     CDS             complement(11415..12758)
                     /gene="mukF"
                     /locus_tag="PWX02_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HDL1729091.1"
                     /translation="MQNELAQTIPELISWTKDREFSLSLSSDRLAFLLAISIYNQEQT
                     DGELLESDLIDLFRYVSNSFEQSDATFTQRANNAINDLVRQRLLNRFSSEFTEGLAIY
                     RVTPLGVGISNYYVRQREFSTLRLSIQLSIVADEIQRASVAAEEGIEQAQDERFWRNN
                     VFAPLKYSVAEIFDSIDLSQRIMDENQHQIRERIAELLSQNWHEAIMSCEQLLDETSG
                     NLRELQDTLNAAGDKLQAQLLRIQSCLMARNDLDFIDQLIVNLQNKLDRIISWGQQAI
                     DLWIGYDRHVHKFIRTAIDMDKNRVFGQRLRQSIQDYYNSPWLLYTAKADSLLDLRDD
                     EAMLNEAESVGELPSELEYESLSDVQEQIVSAMQAHLAPFRAEGKPIDLGVILREQLA
                     RFPESRHFDVARIIVDQAVKLGMASQDSQAVYPQWQPINEQGAEVQANVIDQYNK"
     gene            complement(13016..13903)
                     /gene="galU"
                     /locus_tag="PWX02_001067"
     CDS             complement(13016..13903)
                     /gene="galU"
                     /locus_tag="PWX02_001067"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL1729092.1"
                     /translation="MKVIIPVAGLGTRMLPATKAIPKEMLTLADKPLIQYIVNECVAA
                     GFKEIVLVTHSSKNAIENHFDTSFELETMLEKRVKRQLLDDVRSIVPKDVTLIHVRQG
                     QAKGLGHAVLCGRTVVGDEPFAVVLPDVLLGEFTANQKTENLAAMVKRFQETGYSQIM
                     VAPVPMENVSSYGVADCHGVDIPLGGAAPIAKMVEKPSIEEAPSNLAVVGRYVFSSNI
                     WNLLEKTPVGVGDEIQLTDAIDMLIHQETVEAFHMTGRSFDCGDKVGYMKAFVEYSLN
                     HDKCGKEFKAFIKELAKSL"
     gene            complement(14039..14224)
                     /gene="csrA"
                     /locus_tag="PWX02_001068"
     CDS             complement(14039..14224)
                     /gene="csrA"
                     /locus_tag="PWX02_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDL1729093.1"
                     /translation="MLILTRKIGEVLLVGDDVEITVLSIRGNQVKLGVNAPKEISVHR
                     REIYERIKASVEEENAE"
BASE COUNT         4011 a         3282 c         2687 g         4248 t
ORIGIN      
        1 gctaccgaaa cagcaagcac aactgcatct ttaccgccaa ccgttgctct tgctgcatct
       61 tggtatttat ccagctcaac agtcgcaata tcttttaatt tgatggtttt tccatctttg
      121 gtggttgaaa ccgtaacctc ttctaactct gtcacagaag gcgtggtaga aagtactttg
      181 tttctataaa ccgtatagta accgttagaa ttacccgcag cggtttgcaa gttattggca
      241 cttaatgccg ataatacagc cgcccctgat aggttattgc tcgccatttt atccggatca
      301 agccagatgc gtaagccaaa aacagagcca tacacatcaa cgctagaaac accatttacc
      361 gtaaagaact gtggttttac tacacggtta atataatcgg taacttgtga tgtattaagt
      421 tcatcagaga caaatcggat atacattaac gcatcatttg aaccggttga gactctaatt
      481 gacggatcat caataccgct cggtaaattt gagcgtaccg cattcacttt tgccgaaata
      541 tcggaaagtg cggcattcgg atcggtattt aacttcattt ttgccgttac cgtagtggta
      601 cttggagagc ttgatgaagt catatagtca atattatccg cttgagcaac agcctcttct
      661 aactgtgagg taaccagtgt ctgcattaaa cgtgcatctg cacctgaata gttaacactc
      721 acggttacaa tcgagaccgt catctccgga tattcacgca cctgcaattt ttcgattgct
      781 tgcaagccta ggatcgtaat caataggctg atacaaactg ccaaaacagg acgtttaata
      841 aagatgtcag taaatttcac ttaactcact ccactacagt ttactgtttt tcgccggtgt
      901 ggtaatgcct acggcttctg tatcagaaac tttaaccaac gcattattac ttaaacgttg
      961 taaaccgcct gttacaatca gatcacctgc tttcacgcct ttggataatt gtgaatagtt
     1021 tcctttgcgg tcagccgttt tcacttccat ctgttttgca cggtacattt tgtttacatc
     1081 taaacttggg ttttgtaccg ccattttgtt caccatttct ttatcttcat cagataatgg
     1141 ttgcagcaca tataccgttt caccatacat agtgtaagcg atagaaattt gtggtacgac
     1201 gatttgtccg gtttctgttg gtaaagcaac atttaagcgg gcaaacatac ctgaaagcaa
     1261 tttttcctgc ccttcttcaa tcaccgcttc cacattaatt aagccggtta aacgatccac
     1321 agccggatca atagccacaa ttttagctgg gaaggtttgt gccggtaacg catcaattac
     1381 ggctgtcact ttttgcccaa tcgagatttg ctctaaatta gtttgcggca aggtaaaacg
     1441 tactttcatt gaagattgat cttccacacg cacaatttcc gtacccattg taatgtattg
     1501 cccaacattc acattcacga tacccgctaa accactaaac ggagcataaa tttgacggcg
     1561 tttaatcgta gcacgcaatg cctcgatatt ggctaataat tggttgtagg tagattgagc
     1621 attatcaaac tcggctttag aagcactgtt tgaggcaatc agattacggt aacgctctaa
     1681 atttgctttg gcattcggta attgcgcttc agaggctctc aagttagcaa tttcaacact
     1741 gctgtcaaat tctactaaca gatcaccttt tttcacacgc tgtcctgaag tgaccaaaac
     1801 acggctaacc acgcctgaag tttcagcact taacattgcc ccttgatttg ggcgaatata
     1861 accgaccgca gaaatggttg gtgtccattc ttgggtcgtc acttgcatag cagtaacttc
     1921 gctcaccgcc tccggcatat ttgccgctgc ttcggctttt ttcattgccc cgattttttg
     1981 taaccctacg attgcagcaa acacaatcaa tacgattaac aatgtgagta caattaaaaa
     2041 ctttctgcct ttacccagct tctgtgtttc cgttgtcata cattctccat aaaactaata
     2101 aaaagaaact taaaaagtga ttgctttcca agtgcgtaaa ataatctcgt ctaaatatcc
     2161 atcaaactga ttttcttgat tcagattttg cagatattgg atatccacca atgtgccgat
     2221 gcttaatcca taaagcaact cattaggtag atcagcaata atcctttgct ttttcccttc
     2281 taaaattaat ttattccaaa aggaagtttc atcagctatg gtttcccgaa tcaattcatc
     2341 aatccctatc accgcttgaa actgttttac caaatctaaa cgctcgatta aaacctgcca
     2401 ttttttattg accaacgctt tatattgctc aaataacgcc atttcaggct taaactcaaa
     2461 atgcatataa ccgttataac attcgagtaa ttgggcagcc aatgagtgca ataaatcatc
     2521 tttggtttta aagtaaagat ataatgtacc ggaagcgatc cctgcttctt ttgcaatact
     2581 acgcattgaa aggttttgca aaccttcttg cacaatcagc gactcggttg ccgataaaat
     2641 acggtttacc atctcttctt ttggctcgct tcgcctagac attttatctc cttacctata
     2701 atgaacagcc gttcatttta gcttttttta tgaaaaaaga aagcagaaaa cacctatcaa
     2761 attaaaataa aatagttgca aaattcctca tttattgaac aagcggtcat ttttggctaa
     2821 aactttgcaa aaatcactca aacaataacg cttaacgcta ctttattatt atttttgtgc
     2881 tactatttgt acctaattat aggatcatct ttatttcaat atggcaacga aatctaaata
     2941 ccacaccaaa caatttgagc agctattggc tgacctcatc gccaccatag aaaagcataa
     3001 agcaccggtg gatttatcgt taatggcatt aggcaatatg ataactaaca ttttaattga
     3061 aaatattcaa agcccggcac aaagagaagc tctcgctgat gcctttgccg atgcattaaa
     3121 acaatcacta aaaaccaaat aagatgatgt ttgcacgcct acgcagttta ttgccgacca
     3181 attctcgcca atatcgagaa gaaacctcac agaaaatcac gtgggggcat tggtttgcac
     3241 tatttaatat tgtgttggca ctactcattt cctctcgtta cgcatttaat gcagactggc
     3301 cgaacacgct tgtaggcaaa ctttatttct ttgtcagcct gttcgggcat ttcagctttg
     3361 tggtctttgc aggatttcta ttaattctgt ttccgctcag ttttattgtt aagaatcatc
     3421 gcacctatcg tgggctttcc gtgattttag ccaccatcgg gcaaaccgtg ttattggtgg
     3481 atactgaagt cttcaagcaa ttttatctgc atttatcacc gcttgtatgg gatctgttag
     3541 ttaatccgga agaaggggaa ctgacccgta aatggcaact gctgtttgta ccaatgccac
     3601 tccttttgct ggcagaaatg ctctactctc gctggtgctg gcagaaactg cgtagtttta
     3661 gccgccaaaa atggggcaaa tatgtcgctc tctttttcgt gatcagcttt accgctactc
     3721 atttaatcta tgcgtgggca gatatgaatg cctaccgccc gattaccgct caacgagcaa
     3781 actacccact ttcttacccg atgacggctc gtaatttctt agaaaaacac ggttttattg
     3841 accgagtaga atttaatcaa gaaattgaag aaagtggcag actagatact ttctatctga
     3901 attatccaaa atctcccttg attgcgacca aaacgccctc aactaatatt ttactgatta
     3961 atttatcagg tttatctaaa cagataataa atgccgaaac aatgcctaag ctgacagaaa
     4021 tcagccaaca aagccaccgc tttatgcagc attacagtag tggtgattca gcctctgccg
     4081 gtgcattagg tctattttac agtttaagcg gtaaatattt ggattccgtt ttagccgata
     4141 aaaccgtgtc accgcttatc gaaatcgcta gaaaatcgga ctatcaatta gggctgtttt
     4201 cacataatgg ttttgccgac ccgatttatc atcaggcgat cttctctggt attttattgc
     4261 ctacggcgca cagtaatgta ggggcgattg ggcaatggaa aaactggatc agctctcgct
     4321 cattggaaaa tccgttcttt agttatttag acttaagaat gcccttcgcc cacaccgctc
     4381 aagagcaggc aaaattatta gattggcaat tcgctgaaat ctggcaacat ttgacccaaa
     4441 atgcactatt ggcgaatacg ctggtaatca ttaccgccga tattagtaat gaagttggag
     4501 cagaaaatag cttcgataaa caacacattc aagtgccgat gatgttctac tggcaaggcg
     4561 aaagcaaaga gtaccatttc ctttcaagcc atttagatat tatgccaacc ttactgagcc
     4621 aattcttcgg cgtgcaaaat ccgctgaatg attatgcaca aggtatggat ttaagcaccc
     4681 agaaccaacg tttatgggta tccagttcta atcacaaatg gaatgtggcg attatgccga
     4741 atggcgatca atatcaaatt gatcgtaaag gcaactttaa gcactttaac agcaatggcg
     4801 aacacgagaa agatgctcga ccaccattag cgttgttctt acaaatgatt caacaaagca
     4861 atcaatttat tgaaaagtaa tgttcaacaa gcggtcattt ttgcaggaaa atttgcaaaa
     4921 tttcagcaaa atatgaccgc ttgtcactat ctgagctgtc tatatttcaa gctaacaaat
     4981 acaaaccaaa ccattgctac aaaagctgct ccccagtaat gctccaccgc caatgcctaa
     5041 attataagtg ccggaaaaaa ttgcagttgc gacatcagtc gtatcagggg caagttcaag
     5101 caccttcatt tgcaacgcaa tgctcattcc ggtaatgcca attccccaga caaaagcgac
     5161 cataaacatt gccactgttg agtcactcaa aaacagtaaa caaagctggc tgcacataat
     5221 aatggcgatc ccgcttaaga taaatttatt cggggctttg ccatataacc gaccaaataa
     5281 aaaacttgcc accacacctg ccaagccaaa aaccagcaaa ataaaggtgg tgttttccac
     5341 actcaccgaa ctgattttaa tcataaacgg ctcaatatag ctgtaagcgg taaaatgtcc
     5401 tgaaacgatc aatgccacca gcaaataaat gccgattaac atcgggcgtt taaataacaa
     5461 aggcaaactt tttatcgagc ctgcattttt gcttggtaaa taaggcagca accgccaaat
     5521 catcattagc acaatcaaag cgataatccc aataatgcca aaggttgccc gccagcctaa
     5581 ggcttgcccg atcattcgcc cataaataac atcagcaaca gccgttttct ttccatattc
     5641 gccgtgagta acatcaacgg taacgataat aaaaatacca cccacgcata aaccgtcatc
     5701 ataatgcctg ccgatgaagg ctccatttgg aaactgcttg caatgtctga aagcaatgca
     5761 ataggaataa attcggtggt gtttactata aaagccgcaa aggcaagact aaatactcta
     5821 aggaacgata atctccgttg agcttttcga gcaagtttca tattaattaa atccgagaaa
     5881 aacacattta ttttcaaaat tttaaaaata aatgtaaaaa caaaatcaaa tttccaaatt
     5941 tctgaaaata aaaagattgc caatgtttcc atcaacaatc tttttataca tatttagatt
     6001 aaagtgctaa gttttattta gcaaagcctt ttaaaccaac aacgtgaaca tactcttggt
     6061 tattggtgat tttacgcacc aatttataag ttgtgcctcg ttcagggcta atattttcag
     6121 gggctgcaat taataactgc atatccaaac gttcgcagag ttcaaatagc gtattaattg
     6181 aggtagcatc taatcgagcc gcttcatcta agaacaataa gcgagccggt agaatatctt
     6241 tggcacgcat acggcgagct tcttcttccc agctttgaac taccattaag agaatcgaca
     6301 tacctgtacc gatcgcctca ccggtcgata atgcactact ttctgcacgc atccagccgt
     6361 ctgcaccacg gaaggtttcc acttctaaat ctaagtagtt acggtaatcc aataactctt
     6421 caccaatggt ctgtggtgta cgttgcccca tctcaatatg agggttcacc cgtttataca
     6481 acatcgctaa ggcttcggag aagctcagtt tttggctgtc gaataaatct ttgtgttcct
     6541 cacgattatt ggataacgca ttgagtaaaa tagcgtgcgt atctcggata ttcaccacca
     6601 aacgcacccc ttttacctga ccgaatgcaa tattttgtaa accttggttc agttgcaaaa
     6661 tgcgttgttg ttcacgctga atggttttac gtaaaatatt tgccacactt tccgcactga
     6721 tcgccagttt tttctcacga ccggttaatt cattggttaa gcgtgaaagc tcgatttcca
     6781 tctgctcaat cgcatcaatc ggatcatcgg ttttgataat gtcttggcga atccgctcac
     6841 gcaaatgctg ataaaccgca ataaagaacg ccactttgtt ttccggcttg cgactatctt
     6901 cagaagcccg caagctatca cgtaggtatt cattatccgc cacggcggtg cgtaatgccc
     6961 cgagagcttt atccgaaatc gaacgtaatt cttcagccga ttgataagcc aattcacggc
     7021 gatttaaacg tttttccaca tcgctgttac gagataaacg ttgcacaata caccagctgg
     7081 cttttgcctg caccaccaat tcacgttgtg tatgataatc acgttcggct ttacgtaagg
     7141 tgcggttaag gttatccatt tccgcctcga tcaccgcaat ttgtttatct aaataacctt
     7201 tgcgggaacg ttgctggctt aaacgttgtt gcaattcatc acggcggaat ctggctcgct
     7261 cttccgctcc gctatcacca cgcacgccca aatcgtcaat ttcacgaatt aactcttgta
     7321 gcatttggtt tttcgcctca taagaactgc gtaagccggt caatacttgg ttatattgtg
     7381 aaagttgagc ttgagcttga cgcagctgat cacgagcctg ttcacgctca cgttgtgcat
     7441 tttccaaacg ttggcgtagc tgttcggtta atgcagagcc ttctgtaccc accgtttctt
     7501 gatagctaaa atggaggcgg cgattcatta catcagccaa gctgaacact ttttgctgta
     7561 acactttttg ctcggcaact gaacggcggt aatcggcttc caactgttca aataaagccg
     7621 gatcactttt taatgcggct gcaatcggct caagttggct taatgttgct ccaaattggc
     7681 ggataaacat ttcatcttct tctgccaatt ctaattgctc acgacattcc tccgctcgtg
     7741 cttccagcgt ttcatcggct aataaattcg caagcggtag aattttattc aacatttgca
     7801 actgggcttt tacgctttct aattggtgac gtaattgctg ttcgttaccg gctgattgtg
     7861 ccaattcacg ttcaatttct gcccgttctg ccgcaatctc ttgcattaac atttcagggt
     7921 tttcttggaa cgctaaattt aagtgcgtgc cgataaattg gcttaagtgt tgatgtaaac
     7981 gctggcattt ctgcacatca aaggcacgtt cagcgtgttt ttctgcggtt tcatcacgct
     8041 ctgcttttaa agtcgctaaa tgtttttcac gagcagcacg tccgaataac ggcacttcag
     8101 ggaatttaga ataacgccat tggcgatcgg agactttcac caccacgcct tcgcctaatt
     8161 cctcggcact aaataccgca tcgtcaaagg cggacggatc accttcaatt aaataaagat
     8221 cacttgggca atcgtctaat ttctctagct gtgttttaac cgcctctaaa tcacgcacta
     8281 caatagcgtg gcgagcctcg ccgtataagg cggagaaata aggcgcatct tcaattgata
     8341 catcatcata taactcagag agtaacactc caccaaaacg ttctgccaac tggtttaagc
     8401 gggcgtcttc cgagccgtcc ggctggctta agcgattaat ttgctcatca agcagttgtt
     8461 ctttacgagc caattcatca cgagcaaggg tggcttcacg ctctttgctc agcatatttt
     8521 gcataaactg catcactgat tggctggctt caaacttctc gccacattgc tcttctaaac
     8581 gagtcagagc cgattgtgcc gtatgccaag ccggtgcatt cttcgctaat tgctgatatt
     8641 gttgattaag ctgttcacgt tgttggcgtt gggttgaacg ttgttctaca aagtccgaaa
     8701 gctctgcctc taaatcttct aggcgagctt gctgctcttc aaaataggcg tctaattctt
     8761 ccgctgaatt gagttgttgt tgcgattttt ggttaaattc cgccactaaa cgctgggcat
     8821 tttgctgttg ttgcaaacgc tgttctaaat cgttgagttt ttgacgtaaa ctcaccgctt
     8881 gctgagcttg cattttttgc tcagggaagg cagaaagtaa cgcacgagcc tcttcccacg
     8941 cttgcaagcg gtcaatttca ccggaaattt tgcaaaccga ttcaaacgct ttttcaaatt
     9001 gggttttcgc catatccgac acgcttaaac gttgttccag ctcaaatact tgatcggtaa
     9061 gatcttcggc ttgagccaca aattctgcgt ggtaatcttc aatattattt aaatctaaat
     9121 tcactaagcc ggttagctgt ttggcttttt ctaacgcatt caccgcttgt tggtattgca
     9181 atgcacgagt ttgctgtgca tctaaggctt gttggtaatc cgccatttga gaacgcaatt
     9241 cttcaacgta atcatctgcc tcatccgcac gagcctgagc attttcggca atttccgcaa
     9301 tttcttccaa tgcctcttgc tgttcttcca gcttaatatt cagctcttca acttcgtctt
     9361 ggtagcgttc gattttctct tggtggcgta acgcattcat cactaaattt agatgatcgt
     9421 tcgcactatt gtaatccgcc tctaacgcct gctctgcttc ggcaatatcc gcactttcac
     9481 ggctaaattc cacaaagcgt tgctgttcga gcaaaatttt ggattttgcc gcataccact
     9541 ctttgcgttg ctctaaggct tgttgtacat tgccacggcg ttcattcgca ttacgcatat
     9601 aatccgccga cacataattg gtcgattcgg taattaagcg tttgaacata tcccgatccg
     9661 attgcgtgac cttaatcgcc tctaaggtca tacggttttc acgcagagcg gattccatat
     9721 cttggaacgc ttggcgaacg cccgtatttt ccggcaacaa gtaatcacgc aaggatttgg
     9781 tgatcacact tgaaatacca ccataaagcg aggcttcgat taatttgtag aatttgctac
     9841 ggtcggctgc cgaacgaagg cgttttggga ttacgcccaa atcgaacata aaactgtggt
     9901 aatcagtaat cgaatggtac tgtttgaatt ggacttcgct gttctcaaat tgatctttga
     9961 gttcatttaa cgccagcact cgagccttat tccccacttg ttcggtcagt aggctgataa
    10021 tgctttgctc ttgaggaata ttctggagcg agaacgaacg aatatcgact tttttatccc
    10081 gtccgacaat ttgttgcaaa cgcacgccgg taataatccg ttgcccacgg gaattgaatg
    10141 actctaacac cgcatagcaa acacccgctt ttaacttacc gtataagcct ttatcacgag
    10201 aaccggcggt tgagcctgct tcagtcgtat tacggaaatt aagcaaggtt aaatctggga
    10261 tcaagacggt cacaaaccct gccattgtgg tagacttacc tgccccgtta ccgcctgaaa
    10321 gcgtggtcac taagtcatct aaatcaaagg ttcgagcaaa aaaaccgttc cagttaatca
    10381 gcgttaaaga gagaaattta ccgcgttgta catcattcgg gcgtgcaagc ggtgcaattt
    10441 gaggattatt ttgcaattct gtggtatcaa tcattatgtt ttctcactaa ttgtcctagc
    10501 acgctaaagc gtactaggtt aggtataggt gagtagctcc gctactctaa tcgcaacctc
    10561 agccttggag ggctgcttta tgcttaacct agttcggctt taccgtgtct agggcagact
    10621 cattaaatgc tgaatctatt cgaatgaaat cactcatttt ctccgtcaaa gggggcttca
    10681 tctgcaatat tttcatctat ttcaaccgct tgttcgttta acaacgcagg ggttgtcgct
    10741 tcaccatcac gaattaagcg aagctgtgcc tctcttgggt catcaccggt acgcacatca
    10801 gccccaaaac ggaagaccga ttctgaaatc acaaattttt tgctgttttg ctcgcctact
    10861 cgggtaataa tgccgatacg ggctaatctg cgtaacgctc cgccgacttt ttctgccagc
    10921 ttggcacggt ctaaatcgga accggtcgaa cgtgggttta ccgcttttaa taatttgctt
    10981 tcatcggcaa gactgagtaa ttcttcatac acatcatctt ggctgaaaat cccctgctga
    11041 gccaaacgtt ccggacttaa atagaggtag cacaacacct tacccactaa catttccatt
    11101 tctgacattg ccgaacgggc aattagtgtc gaggctttcg ggcgtaggta gaaaaagcct
    11161 tccggtgctc gaatcaactc aacgtgataa cggcggtaaa aactctctag ttccgcttga
    11221 aaatccatca aaaagacgtg ttggtcaagc tgctcggtat tgatatggcg accggctcgt
    11281 aattggctgt ctagctccgg aaaaatcggg ttggcaatcg caagtgccag tttcggggaa
    11341 atgaaatctt ggatattctc tgtcattgtc attctcttca attttgcaaa attctgggta
    11401 aaaacgaccg cttgttattt attgtattgg tcgataacat tcgcctgcac ttccgcccct
    11461 tgctcgttaa tcggctgcca ttgtgggtaa acggcttggc tatcttggct tgccatacct
    11521 aacttaacag cttgatcgac aataatacga gccacgtcaa aatggcggga ttccgggaat
    11581 cgggcaagct gttcacgcaa gataacgcct aaatctatcg gtttaccttc agcacggaac
    11641 ggcgctaaat gagcttgcat tgccgaaaca atttgctctt gcacatctga aagcgactca
    11701 tactctaact cacttggcaa ctcgcccacc gattctgcct catttaacat tgcttcatca
    11761 tcacgcaagt ccagtaacga atcggctttt gcagtataaa gcaaccaagg gctgttgtaa
    11821 taatcttgaa tcgattggcg taaccgttgc ccgaatacac ggtttttgtc catatcaatc
    11881 gctgtacgaa taaatttatg cacatggcgg tcatagccga tccaaagatc gatagcttgt
    11941 tgtccccagc tgataatacg gtctaactta ttttgtagat tcacaatcag ctgatcgata
    12001 aaatccaaat cattacgagc catcaggcaa ctttgaatcc gcaataattg agcttgcagt
    12061 ttatcaccag cggcatttaa ggtatcttgc aactcacgca aattccccga ggtttcgtct
    12121 aacaattgct cacaactcat aattgcttcg tgccagtttt ggctaagcag ttcggctatt
    12181 cgttcacgga tttggtgctg attttcgtcc ataatccgtt gtgaaagatc gatgctgtca
    12241 aagatttcgg ccaccgaata tttcaatggg gcaaacacat tatttcgcca aaaacgttca
    12301 tcttgtgcct gctctattcc ctcttccgct gctactgaag ctcgctgaat ttcatctgcc
    12361 acaatcgaaa gttgaatcga taaacgcagg gtagaaaatt cccgctggcg aacgtaataa
    12421 tttgaaattc ccacccctaa cggcgtaaca cgataaatcg ccaaaccttc cgtaaattcg
    12481 ctactaaaac ggtttaacaa ccgttgtctg actaaatcat taatcgcatt attggcacgc
    12541 tgggtaaaag tggcatcaga ttgctcaaac gaatttgaaa cataacggaa aagatcaata
    12601 agatcacttt ctaacaattc accatctgtc tgttcttggt tataaataga gatagcaagt
    12661 agaaacgcta agcggtcgga agaaagcgaa agtgaaaatt cacgatcttt cgtccaagag
    12721 atgagttctg gaatagtctg tgccaattcg ttctgcatac gggcttaatc tttaatttct
    12781 atacaaaaat ggtatttatt gtaactgaat gtaatcaaaa tgggaatgga taaaacgcag
    12841 acaagagaaa tgaacaatat aagcggtcaa atatgggtga ttttttgcaa aaagtatttt
    12901 aaaggtaaat gaatccgaaa atttattatc actacgctaa tttatgatac cataaataga
    12961 aaggactgga tccaatagaa tatcagacct tagatcatag tctaaactct aaggcttaga
    13021 gtgatttagc taactcttta ataaatgctt taaattcttt tccgcatttg tcatgattta
    13081 aactatattc tacaaatgct ttcatatagc cgactttatc accacaatca aatgagcgcc
    13141 ctgtcatatg aaaagcttca accgtttctt ggtgaatcaa catatcaatt gcatccgtca
    13201 gctggatttc atcacctacg cctactggcg ttttttctaa taaattccaa atatttgagg
    13261 aaaatacata acgtcctact accgctaaat tagaaggcgc ctcttcaata cttggttttt
    13321 caaccatttt cgcaataggc gctgcccccc cgagagggat atctacacca tggcagtcag
    13381 caaccccata gctacttaca ttttccattg gaaccggcgc aaccataatt tggctatatc
    13441 cggtttcttg gaaacgcttc accatcgcag ctaaattttc cgttttttgg tttgctgtaa
    13501 attcaccaag taatacatct ggtaatacaa ccgcaaacgg ttcatcgcca accactgttc
    13561 gtccacatag aaccgcatga cctaaacctt tggcttgccc ttggcgaacg tgaattaagg
    13621 taacatcttt tggtacaata gaacgtacat catctaataa ttgacgtttt actcgtttct
    13681 ccagcatcgt ttccaattca aaagaggtat caaaatggtt ttcgatagca ttctttgaag
    13741 agtgagtaac caatacaatt tctttaaaac ctgcagccac acattcattt acaatgtatt
    13801 gaataagagg tttatctgca agggtaagca tttcttttgg aatagctttg gttgcgggta
    13861 acatgcgcgt tcccaatcca gcaacaggaa taatgacttt catttggaat cctttgatga
    13921 cttaacatat tagatataac tgaaataaac cgcttagaca taacaagcgg tcaattttca
    13981 gaaaattttg cattgcctgc ttcgctgtta tcatctagca gctttacagt taaaatgctt
    14041 attccgcatt ttcctcttct actgaggctt ttattctttc ataaatctct cggcggtgta
    14101 cagagatttc ttttggtgca ttcacaccta atttaacttg atttcctcgg atgctgagta
    14161 ccgtaatctc aacatcatct cctacgagta aaacttcccc aatcttacga gttagaataa
    14221 gcataatt
//
LOCUS       DANJWC010000048        13911 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.048, whole genome shotgun sequence.
ACCESSION   DANJWC010000048 DANJWC010000000
VERSION     DANJWC010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13911
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.048"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..697)
                     /gene="rbsC"
                     /locus_tag="PWX02_001069"
     CDS             complement(<1..697)
                     /gene="rbsC"
                     /locus_tag="PWX02_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005753457.1"
                     /note="functions to transport ribose at high affinity;
                     forms a complex with RbsA2C2B; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDL1729094.1"
                     /translation="MATQTKQFQLGRFLIEQRSFVALFILIVIVSMINPDFFSVDNIL
                     NILRQTSVNAIIAVGMTFVILIAGIDLSVGSVLALTGAVAATLVGSELPILLVIPLVL
                     LLGTAFGGISGAIVAKGKVQAFIATLVTMTLLRGITMVYTDGRPISTGFSDQADAFAF
                     IGTGYLFGIPVPIWIMAVVFGLAWYILKHTPIGRYIYALGGNESATQLSGINVNKIKI
                     FVFAVSGFLSALAG"
     gene            complement(707..2203)
                     /gene="rbsA"
                     /locus_tag="PWX02_001070"
     CDS             complement(707..2203)
                     /gene="rbsA"
                     /locus_tag="PWX02_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HDL1729095.1"
                     /translation="METLLKMSGIDKEFPGVKALSNACLSVYGGRVMALMGENGAGKS
                     TLMKVLTGIYSKDAGSISYLGKEVTFKGPKDSQEAGISIIHQELNLVGNLAIAENIFL
                     GREFKTAWGAIDWKKMYAEADKLLARLGVKHSSHQLCAELSIGEQQMVEIAKALSFES
                     QVIIMDEPTDALTDTETASLFKVIRELKAENRGIVYISHRLKEIFEICDDVTVLRDGQ
                     FIGERVIADIDEDQLIEMMVGRRLEEQYPHLHQERGELLLEVKNLSGSGVNNVSFQLH
                     KGEIVGISGLMGAGRTELMKVLYGALPKTAGSIELNGKAISNHCPQDGLNNGIVYISE
                     DRKGDGLILGMSVKENMSLTSLDHFSKAGSIRHDAEKLAVDDFIEMFNIKTPSREQQI
                     GLLSGGNQQKVAIAKGLMTRPNVLILDEPTRGVDVGAKKEIYQLINEFKKDGLSILMV
                     SSDMPEVLGMSDRILVMHEGQISAEFSRAEATQEKLLAEAIGKSTGLN"
     gene            complement(2213..2632)
                     /gene="rbsD"
                     /locus_tag="PWX02_001071"
     CDS             complement(2213..2632)
                     /gene="rbsD"
                     /locus_tag="PWX02_001071"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005753460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDL1729096.1"
                     /translation="MKKTLNLNAQLSHSIATLGHTDSLTVCDAGLPIPNTIERIDLAL
                     TAGVPSFLQTFDVVVSELFVERVLLAEEIKHKNPQVLAALLERLTQLEQAQQNKIQID
                     YVNHEIFKEQTHYSKAVVRSGECSPYANIILYSGVPF"
     gene            2862..3122
                     /locus_tag="PWX02_001072"
     CDS             2862..3122
                     /locus_tag="PWX02_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HDL1729097.1"
                     /translation="MALLITHKCTNCDMCLPECPNEAISVGDDIYIIDPVLCTECVGH
                     YEKPTCQKVCPITNCIIKDPDNIETEEQLWERFVMIHHPDQI"
     gene            complement(3154..4044)
                     /locus_tag="PWX02_001073"
     CDS             complement(3154..4044)
                     /locus_tag="PWX02_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HDL1729098.1"
                     /translation="MAMNFKQVSQSYPNLCVPLSQYCLIEIAGVDAEKYLQGQLTCDV
                     AKISVGEHTLTSHCDPKGKMSALLRLYRAENEKFMAIIHRSLLPEALTQLKKYAVFSK
                     VTFTELDIPLYGMAGEAAFAKLSENMTALRLTTGQPRAIVWGEELETTADEQLWTLMD
                     IQDGIPVLLQQNQFELIPQATNLQALESAISFTKGCYIGQETVARAKYRGANKRALFT
                     LAGNFESEISLPEVASAIEMQLGENWRATGTVLTSATDENTLWVQVVLNKDVEPNTKF
                     RANGVALEMVELPYSLAENE"
     gene            complement(4053..4916)
                     /locus_tag="PWX02_001074"
     CDS             complement(4053..4916)
                     /locus_tag="PWX02_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011272075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDL1729099.1"
                     /translation="MIYSMTAFAHLEMKKEWGNAVWEIRSVNQRFLETYFRLPEQFRN
                     LEMTLRERLRSALTRGKVECSLRIDLSKTQNSEIALNKEYAAQVVQSLKWLKETANEG
                     EINLVDVLRFPGVVDNESQDLDQIAQDLLAGFEQILADFIAMRAREGENVQALILQRL
                     EAISVEAAKVQAQMPEILQWQKDRLQQRFDELNLQLDPQRLEQEMVLLAQRVDVAEEL
                     DRLQLHVKETTNILKKGGAVGRKLDFMMQELNRESNTLASKSINADVTNSAVELKVLI
                     EQMREQIQNLE"
     gene            5211..5927
                     /gene="rph"
                     /locus_tag="PWX02_001075"
     CDS             5211..5927
                     /gene="rph"
                     /locus_tag="PWX02_001075"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDL1729100.1"
                     /translation="MRPNNRANNQVREIKITRNYTRYAEGSVLIEFGETKVLCNATVE
                     ESVPRFLKGQQQGWVTAEYGMLPRATHSRTQREAAKGKQGGRTMEIQRLIARSLRAVV
                     DLKALGERTVTVDCDVIQADGGTRTASITGACVALYDAMNKLVADGTLKTNPMKGLVA
                     AISVGIVAGEAVCDLEYLEDSNAETDMNVVMVEDGRLVEVQGTAEGEPFSHQELLTLL
                     DLAKQGISQLVEAQRKALAE"
     gene            6151..8450
                     /locus_tag="PWX02_001076"
                     /pseudo
     CDS             6151..8450
                     /locus_tag="PWX02_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005759321.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(8528..9229)
                     /locus_tag="PWX02_001077"
     CDS             complement(8528..9229)
                     /locus_tag="PWX02_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HDL1729101.1"
                     /translation="MDLQNRENFLNKLAQRMGRPRQNVPEPIPVLENNHAITRLTDRP
                     QPQLCQEFIDFARVMMADVVEAKEADLPQAIIDICEKYGGASVIVNNDQRLKDLGITA
                     AIQAKYEDSYVWDFNQAETNFEKARTANIGVVYGEYGLAESGGIVLFSDKEFGRSVSL
                     LPEKSVVVLRKSTVLPRVAQLAQILHEKAQRGERMPSCVNIISGPSATADIELIKVVG
                     VHGPVNKIYLVIDDL"
     gene            complement(9234..10643)
                     /locus_tag="PWX02_001078"
     CDS             complement(9234..10643)
                     /locus_tag="PWX02_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HDL1729102.1"
                     /translation="MSYLQTNTLPFKQRVEQQVNNEIVRKALVKAQETIGANRQRMVD
                     ELGNWEEWRDDAKAIRNHVLANLDAYLYQLSEKVTQNGGHVFFAETAEEATDYIKNVA
                     KAKNAKKIVKSKSMVTEEIGMNHVLEAEGIKVVETDLGEYLLQIVGDKPSHIVVPAIH
                     KDRHRIRQEMHDVLGYQGSETPEEMTAFVRQKIREDFLEADIGISGCNFAVPETGSVC
                     LVTNEGNLRMATTVPKTHIAVMGMERIAPTFKEVDVLITMLARSAVGAKLTAYNTWLT
                     GPRLEGETDGPEEFHLVIVDNGRSKILESEFQEVLRCIRCGACLNTCPAYRQIGGHGY
                     GSIYPGPIGSVISPLLGGYEEFKELPYACSLCTACNSVCPVKIPLAQLILKHREKIAD
                     AGLTPMAERLSIFGFNFANSHPTLWKVGVKVGAKVASKLIKNGKAPVNFGALGEWTKA
                     RDLPTAEGESFREWFNNRG"
     gene            complement(10645..11376)
                     /locus_tag="PWX02_001079"
     CDS             complement(10645..11376)
                     /locus_tag="PWX02_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HDL1729103.1"
                     /translation="MNVNFYVTCIADVMKAGVAKNTVLLLEKLGCKITFLEKQGCCGQ
                     PAINSGYTKQALAGMKNLVETFEANDYPIVAPAGSCVYAIKTYPDHFERFGEKEWAER
                     AKKVADRFYDLTDFIVNKLGVKDVGAYLPGKAVYHPSCSLFRKLGIKDEPIALLQHVK
                     GLELLPIKNQETCCGFGGTFSVKMAEISGEMVKEKVANVDTDEPDYLIGADVSCLMNI
                     GGRLSREGKPIKVMHIAEVLMQGGK"
     gene            complement(11520..13124)
                     /locus_tag="PWX02_001080"
     CDS             complement(11520..13124)
                     /locus_tag="PWX02_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDL1729104.1"
                     /translation="MALFLSVFPIVLLIYLMVKRNALPSYVALPLTALLIFVLQLTYF
                     GNDTTLIFANIIAGLGDVLTPITVIFGAILFNRFSEVSGATNTMRKWLGTINPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILVGLGFKPLQVAMLALVMNSVPVSFGAVGT
                     PTWFGMGPLLKDGLLTDAQVLEIGSITALIHSIAAFIIPVMALRLIVSWKEIRQNIVF
                     IYISIFACVIPYFIIAQFNYEFPSLVAGAIGLLVSVWVANMGIGLAKSENHLDGDKAT
                     FGEVAKALLPTGLLIFILVITRIQQLPLKAMLNDATAWIVSSLGFANFEISQGLIFAL
                     KNILGTNVATSYKLLYVPALIPFVVTVLICLPIFSVSGKNTQEIFGTSFKQVKKPFLA
                     LLGAIVMVKLMLIGGVDSMVQIIGKNFAEFTGQSWTLFASFLGAIGAFFSGSNTVSNL
                     TFGTVQYATAQQVGLSVTLILALQSVGGAMGNMVCINNIVAVCSVTGIEKQEGNIIKK
                     TAVPMFVYGVIAAISALFIVPMFYNL"
     gene            complement(13415..>13911)
                     /gene="ychF"
                     /locus_tag="PWX02_001081"
     CDS             complement(13415..>13911)
                     /gene="ychF"
                     /locus_tag="PWX02_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDL1729105.1"
                     /translation="KPTMYIANVNEDGFENNPYLDRVREIAEKEGAVVVPVCAAIESE
                     IAELDDEEKVEFLQDLGIEEPGLNRVIRAGYRLLNLQTYFTAGVKEVRAWTVSVGATA
                     PKAAAVIHTDFEKGFIRAEVIAYQDFIDNKGEAGAKEAGKWRLEGKDYIVQDGDVMHF
                     RFNV"
BASE COUNT         4133 a         3135 c         2595 g         4048 t
ORIGIN      
        1 atccggcaag agcagataaa aaaccactca cggcaaatac gaagatctta attttattca
       61 cattgatgcc tgaaagctgg gttgccgatt cgttgccacc taaagcgtaa atgtaacgcc
      121 caatcggggt gtgtttcaaa atataccaag ctaacccaaa cactaccgcc ataatccaaa
      181 tcggcacagg aataccaaat aaatagcctg tcccaataaa cgcaaaggcg tctgcttggt
      241 cagaaaagcc ggtgctaatc ggtctgccat cggtataaac catcgtaatc ccacgcaata
      301 aggtcattgt gaccaaagtc gcaataaatg cctgcacttt acctttagca acaatcgccc
      361 cactaatacc accaaatgcc gtccctaata ataaaaccag cggaatcacc aacaaaatcg
      421 gcaattcaga accgactaag gtcgctgcaa ccgcaccggt taaggctaaa accgaaccga
      481 ccgataaatc aatgcctgca attaaaatca caaaggtcat tccgaccgca ataatcgcat
      541 tcaccgaggt ttggcgtaga atatttaaaa tattatccac gctgaaaaaa tcagggttaa
      601 tcattgaaac aatgacaatt aaaataaata acgctacaaa agaacgttgc tcgattaaaa
      661 atctgccaag ttgaaactgc tttgtttgag ttgccataaa cacaccttaa tttaatcctg
      721 tacttttgcc aatagcttct gccagtagct tttcttgtgt cgcttctgct cgtgaaaatt
      781 ctgcactgat ttgcccttcg tgcatcacta aaatgcggtc gctcattccc aacacttccg
      841 gcatatcgga cgaaaccatc aaaatactaa gcccatcttt tttaaattca ttaattaatt
      901 gatagatctc ttttttcgcc cccacatcaa caccccgtgt cggctcgtct aaaatcagca
      961 cattcgggcg ggtcatcaac cctttagcga tcgccacttt ttgctgatta ccaccggaaa
     1021 gcagcccgat ttgttgctcc cgacttgggg ttttaatgtt aaacatctca ataaaatcat
     1081 cgaccgccaa tttttctgca tcgtggcgaa tactgccggc tttggagaaa tgatcgagag
     1141 aggtcaaaga catattttct tttaccgaca ttcccaaaat cagaccatcg cctttacgat
     1201 cttctgaaat atacacaatg ccgttattca acccatcttg cggacaatgg ttggaaatcg
     1261 cttttccatt taactctatc gagccggctg ttttcggcaa tgcaccataa agcaccttca
     1321 ttaattctgt acgccctgcc cccattaagc cggaaatacc cacaatttca cctttgtgta
     1381 attggaatga cacattattc accccacttc cacttaaatt cttcacttca agtaataact
     1441 cgccacgctc ttggtgtaaa tgcggatatt gctcttctaa acggcgacca accatcattt
     1501 caattaactg atcttcatca atatcggcaa ttactcgctc gccgataaat tgcccgtctc
     1561 ttagcaccgt cacatcatca caaatttcaa aaatctcttt taaacgatgg gaaatataaa
     1621 caatgcctcg attttccgct tttaactctc gaataacttt aaacaacgat gctgtttctg
     1681 tatcggttaa agcatcggtc ggttcgtcca taataattac ttgagactca aaactcaggg
     1741 ctttcgcaat ttccaccatt tgctgctcgc caatggataa ctccgcacaa agctgatggc
     1801 tactgtgttt taccccaagt cgggcaagca gtttatccgc ctcggcatac atttttttcc
     1861 aatcaatagc tccccaagcg gttttgaact cacggcccaa aaaaatattt tcagcaatgg
     1921 ctaaattacc gactaaattc agctcttggt gaataatgct aataccggct tcttgtgaat
     1981 ctttcgggcc tttaaaggta acttctttgc ctaaatagct aatagagccg gcatctttgc
     2041 tataaatccc cgtcaatact ttcatcaagg tggatttacc tgccccattt tcgcccatta
     2101 atgccattac tcgcccaccg taaacagata agcacgcatt gctcaaggct ttcacgccgg
     2161 gaaattcttt atctatcccg ctcattttta ataaggtttc catcattcca ccctaaaacg
     2221 gcacgcccga atataaaata atattggcat acggtgaaca ctcacccgaa cgcacaacag
     2281 ctttactgta atgtgtctgc tctttaaaga tttcgtggtt cacataatca atttgaattt
     2341 tattctgttg agcttgttct aattgggtta aacgctccaa aagagctgct aacacttgcg
     2401 gatttttatg tttaatttct tcagcaagta gcactcgttc aacaaataat tcactaacta
     2461 ctacatcaaa tgtttgtaaa aaacttggca caccggcggt taaagccaaa tcgatccgct
     2521 ctattgtgtt tggaatcggc aaaccggcat cgcaaaccgt taaactatcg gtatgcccga
     2581 gtgtcgcaat actgtgcgaa agttgtgcat ttagatttag cgtttttttc atactaaccc
     2641 ctttattttc gctccatcac gttggaaaat actccatcga aacgtttcga tggcatcata
     2701 aaaaattttc ttaatcggat aaacaaccaa attgcaaaaa tgtgaagtag ttcaaattat
     2761 tttgctttat aataggtagc atttaatgac aagcggtaag atttgcaaat ttttttgcaa
     2821 aatcgaccgc ttgtttaatt tttattttct caatataaac tatggcttta ttaattaccc
     2881 acaaatgtac caactgtgat atgtgcttgc cggaatgccc gaacgaggcg atttcggtgg
     2941 gcgatgatat ttatatcatc gatccggtgt tatgtaccga atgtgtcggg cattatgaaa
     3001 agccgacttg ccagaaagtt tgcccgatta ccaactgcat tatcaaagat cctgataata
     3061 tcgaaaccga agagcagttg tgggaacgtt ttgtgatgat tcatcaccct gatcagatat
     3121 aaaaaaacgg tcatactctg atgaccgttc gttttactca ttttctgcca aactataagg
     3181 taactcgacc atttccaacg ctacaccatt ggctctaaac ttggtgttag gttcaacatc
     3241 tttattcagc accacttgca cccacagggt attttcatca gtcgcactgg tcaacacagt
     3301 gccggttgct cgccaatttt cgcccagttg catttcaatc gcagaagcaa cttccggtaa
     3361 gctgatttcg ctttcaaagt tgccggcaag ggtaaataaa gcacgtttat tcgccccacg
     3421 atatttcgct ctcgctaccg tttcttgccc gatgtagcaa cctttggtga atgaaatcgc
     3481 agattcaagt gcctgtaaat tggttgcctg tggaatcagc tcaaactggt tttgttgcaa
     3541 gagaacagga atgccgtctt gaatatccat taatgtccag agctgttcat cggcagtcgt
     3601 ttcaagctct tcgccccaaa caatggcccg aggctgtcct gtggttaaac gcaaagcggt
     3661 catattttcg cttaattttg caaatgctgc ttcacctgcc ataccataaa gtgggatgtc
     3721 taattccgtg aaggtcactt ttgaaaaaac cgcatatttt ttaagttgcg tcagggcttc
     3781 aggcaataag ctgcggtgaa tgatcgccat aaatttctca ttttctgcac gatacaaacg
     3841 cagtaacgca ctcattttgc ctttcggatc acaatggctg gttagcgtat gttcgcccac
     3901 cgaaattttt gccacatcgc aggttaattg cccttgaaga tatttctcag catctacccc
     3961 tgcgatttca attaaacaat actgagaaag cggcacgcag agatttggat agctctggct
     4021 cacttgcttg aaattcattg ccataatctg tcctattcta aattctgaat ttgttcacgc
     4081 atctgttcga tcaacacttt cagctccacc gccgaattgg tcacgtccgc attaatcgat
     4141 tttgaggcga gcgtgttgga ttcacggttt agttcttgca tcataaaatc cagtttacgc
     4201 ccaaccgcac cgcctttttt caggatattg gtggtttctt taacgtgtaa ttgcaagcgg
     4261 tctaattcct ccgccacatc aacacgttgg gcaagtaaca ccatttcttg ttctaagcgt
     4321 tgtggatcaa gctgtaaatt cagttcatcg aaccgttgtt gtaaacgatc tttctgccat
     4381 tgcagaattt ccggcatttg ggcttgtacc tttgcagcct caactgaaat tgcttctaaa
     4441 cgttgtaaga tcagggcttg cacgttctcg ccctctctgg ctcgcattgc gataaaatct
     4501 gccagaattt gttcaaagcc ggctaataaa tcttgagcaa tttggtctaa atcttggctt
     4561 tcattatcca ccacgcccgg gaaacgtagc acatcaacaa ggttgatttc gccttcattt
     4621 gcggtttctt ttaaccattt taatgattga accacctgtg cggcatattc tttgtttaac
     4681 gcaatttcac tgttttgggt tttgcttaaa tcaatgcgta agctacactc tactttgcct
     4741 cgtgtcagtg cagaacgtaa acgctcacgc aaggtcattt ctaggttgcg aaactgctcc
     4801 ggcaaacgaa aataggtttc taaaaagcgt tgatttaccg agcgaatttc ccacaccgca
     4861 ttgccccact cttttttcat ttctaaatgg gcaaatgctg tcatactata aatcattggt
     4921 attccttgtg ttggtattcc tgtgttttta taagtaaagc tctctcccct tgagggagag
     4981 agaccgaaaa atatttcgtt cagaaatatt tttcagagag agggaggaat ttgtaaaatt
     5041 tgagataaaa ataaccgctt acccctctcc ctgatttttc ttctgaacga agaaaaatcc
     5101 gtccctctcc cacaaggggc gagggctatg taaattcttt gctaaagaaa aatcacattt
     5161 tactgtgttc tttgctaaaa tggtaaaaat tttagcaaaa ggaaaagaaa atgcgtccaa
     5221 ataatcgagc aaacaaccaa gttagagaaa ttaaaattac ccgtaactac actcgctatg
     5281 ccgaaggctc ggtattaatt gagtttggcg aaaccaaagt attgtgtaac gctacggtag
     5341 aagaaagcgt gccacgcttt ttaaaaggtc agcaacaagg ttgggttacc gcagaatatg
     5401 gtatgttgcc acgagccacc cactctcgca ctcagcgtga agcggcaaaa ggcaaacaag
     5461 gcggacgcac aatggaaatc caacgcttga ttgcccgttc tctgcgtgcg gtggtagatt
     5521 taaaagcctt aggcgaacgt accgttaccg tagattgtga tgttattcaa gccgatggag
     5581 gcactcgcac cgcctcaatt accggtgcgt gcgtagcatt gtacgatgca atgaataagt
     5641 tagtagctga tggaacacta aaaactaacc caatgaaagg tttagtcgca gcaatttctg
     5701 tcggtattgt agcaggcgaa gcggtgtgcg atttagaata tctggaagat tccaacgctg
     5761 aaactgatat gaacgtggtg atggtcgaag atggtcgttt agtggaagta caaggcacgg
     5821 cagaaggcga accgttctcg caccaagaat tactgacatt gctagattta gcgaagcaag
     5881 gcattagcca gctagttgaa gctcaacgta aagcgttagc tgaataattc acttctcttt
     5941 ttgattttct ttttaaataa aatctacatc tgacaaaaca gaataaccgt tctgattcag
     6001 atgtagattt ttttatttcc cttttcaaaa tacactcaat cataaaataa caattatttt
     6061 tttatggaaa tattgcactt cttacaaaat atctgtaaat ttcattgcca ttattttttt
     6121 atggctattt attctagaag gactctaaac gtgttaaaga aaaattatct cactgtatca
     6181 attctccttg ccattagtgg agtaggctac gctaatgaaa tctctttaga gactattact
     6241 gttgatggaa atacgccttc aaccaaaggg aaattactag gtggtgaact aaatagcaat
     6301 gaatctgttg ttgatgaaaa aaattaaaac aaggtagtat tacattaggt aacgctttat
     6361 ctggtgaatt aggcattcat tcaagccagt tcggaggcgg tgcaagtacc ccaattattc
     6421 gtgggcaaga gagtaaacgt gcaaaaatct tacaaaataa tggcgaaaac ttagatatgt
     6481 cgggaatgtc tcctgatcat gcagtaaccg tagatgcgct acttgctaaa cgtattgaaa
     6541 ttttacgtga tccaaccact ttactttaca gtgcaggtaa tacggcaggt gtaattaatg
     6601 tggtagacaa taaaattcca accgctattc ccgaaaaagg ctatgagggg caatttggtg
     6661 tgcgttttgg ctcagccagt aaagagcgtt taacctatgc aggctcaacc tttgcccttg
     6721 gaaatcattt agccttgcgg gtacagggaa tgtataacaa agcttccgaa tactatgctc
     6781 cacactttac gattgaaggc aaaccttacc accgcgtacc agatagcgat gtacaatcac
     6841 aaaccggcac agtaggcctt tcttggattg gagagcgagg acatcttggt attgcttaca
     6901 ctgatcgccg tgataaatat ggattaattg gtcacacaca caaatacgat cactatacta
     6961 ttagtattat tcgccaagcc gttatgtttg caaaaggtta tttacgtttt tatccgcacc
     7021 ttgcagaaga aggagatatt gactataaca accctggtat ccgtttactg catactcata
     7081 ttccgggagg ctcacattat ggacaagata ctcacgagca cggcaaacct tggattgata
     7141 tgcactccaa gcggtatgat attgatggaa gtttgcaaaa tccacttcct ggttttgaag
     7201 aagccaaaat atcagctaac tatgtagatt actatcatga tgaaaaagat ggaaaaagag
     7261 ttgaaaatta cttcaagaac aaaggaaaaa atctacgttt cgaattggtt cacaaagagt
     7321 ggaaagggct aaaaggagct attggtgtgc aatataccaa ccaatctacc tcagcacttg
     7381 cacttgaagc ttcacgagct gcaaaagtat ttaataaaca gcctttactc aataatccaa
     7441 aaacaaaatt atggagtctc tttgcgattg aacgccttaa tttaggtgac tttacctttg
     7501 agttaagtgg gcgggcagaa cgtcaaaaaa tagcaatgga ttatgatgtg aaattaattg
     7561 atcgctggct aggctttaat acacctatgc caaacttgga cccacataaa gacaaaggtt
     7621 attcttatag ctttgcaact cattggtatt tcgcaccaaa ccataagtta acattaaatg
     7681 ctgcacatca agaacgttta cctaatgctc aggaactcta tgctcatggt aaacacattg
     7741 ctttaaatgc atttgaggct ggcaataaaa atctagaaaa agagcgttct aaccaaattg
     7801 agctatcgtt agcttatgtt ggcgacaaat gggattataa attaaatctc taccacaccc
     7861 gctatggcaa ctatatttac ccattaactc ttaatgataa tcgtggacct aaatctttta
     7921 ccgatgaata taacttgaaa gtgaatagat attatcaagg cgaagctcgt tttagtgggg
     7981 cagaaggcga aattggttat ctatttacac caaattatcg cttagccgta ttcggagact
     8041 atgtccgtgg aaagttagtc aacttaccta atattgcaat gagttataac atttggacag
     8101 gtgaagttga caaatgggct tctcagccag acatttctgc acctcgtata ccaccactac
     8161 gtttaggagc aagatttaat gctgacttca acctaaactg gtctggaatg ttagaatatt
     8221 accgagtatt tgctcagaaa aaagtctcga aatacgaaca agttacccct ggccatcatc
     8281 aagtaaacct tggcgtaacc tatagtaatc attttaatca aacagaatac caagtattcc
     8341 ttaaagtgga taacttgctt aatcagaaaa tgtaccaaca tgcctcatat ctaccacata
     8401 ttccacaaat gggtagaaat gcaatgcttg gaatgaatat cagtttttaa tttgtaaatc
     8461 tctacaaaaa tataaccgct tgtaaatttc tatctacaag cggttacttt cagtggattt
     8521 tttgcaatta gagatcgtca attaccaagt aaattttatt taccgggccg tgaacgccaa
     8581 ctactttaat caactcaata tccgcagtcg ccgatgggcc tgagatgata ttcacacaag
     8641 atggcattct ttcacctcgt tgggcttttt cgtgcagaat ttgtgcaagc tgtgccacac
     8701 gaggtaacac tgtacttttg cgtaacacaa ccaccgattt ttcgggcaat aagctcactg
     8761 aacgcccaaa ttctttatcg gaaaacagta caataccgcc agactctgcc aagccatatt
     8821 caccgtaaac cacaccgata ttagcagttc tggctttttc aaagttggtt tcagcttggt
     8881 tgaaatccca cacatagctg tcttcgtatt ttgcttgaat ggctgcggta atcccaagat
     8941 cttttaaacg ttgatcgtta ttcacgatca cactcgcccc gccatatttt tcacaaatat
     9001 caataatggc ttgtggtaaa tccgcctctt ttgcctctac tacatctgcc atcatcactc
     9061 gggcaaaatc gataaactct tggcaaagtt gtggttgagg gcgatcggtt aaacgggtaa
     9121 tcgcgtggtt gttttccaac acaggaattg gctcaggcac attttggcgt ggtctgccca
     9181 tacgctgtgc caatttattt aagaaatttt cacgattttg taaatccatt ttcttatcct
     9241 ctgttattaa accattcacg gaaactttcg ccttcggcag tcggtaaatc acgggctttc
     9301 gtccattcac ctaacgcccc aaaattcacc ggtgctttgc cgtttttaat caatttgctt
     9361 gccactttcg cccccacttt tacgccgact ttccaaaggg ttgggtgtga gttagcgaag
     9421 ttaaagccaa aaatagacaa gcgttctgcc attggcgtta agccggcatc cgcaattttc
     9481 tcacggtgtt ttaagataag ctgtgccagt ggaattttca ctggacaaac actgttacaa
     9541 gcggtacaaa gtgagcaagc atacggcaat tctttaaact cttcgtagcc accgagcaat
     9601 ggcgaaataa ctgaaccaat cgggcccgga tagattgaac catagccgtg accgccaatt
     9661 tggcgatacg ccgggcaagt gttcaaacat gcaccacagc ggatacaacg caacacttct
     9721 tggaattcgc tttctaagat tttagaacga ccgttatcga caatgaccaa gtggaactct
     9781 tccggaccat cggtttcgcc ttcaagtcta ggacctgtca gccaagtgtt gtaagcggtg
     9841 agttttgccc ctaccgcact gcgagccagc atggtaatta atacatcaac ctctttaaag
     9901 gttggggcga tacgttccat acccattact gcaatatgcg ttttcggcac agttgttgcc
     9961 atacgcagat taccttcgtt ggtcactaaa caaaccgagc cggtttccgg caccgcaaag
    10021 ttacagcctg aaataccgat gtctgcctct aagaaatctt cacggatttt ttggcggacg
    10081 aatgcagtca tctcttcagg tgtttccgaa ccttgataac ctagcacatc gtgcatttct
    10141 tggcggattc tgtggcgatc tttgtggatt gcaggtacaa caatatgaga aggtttatcg
    10201 cccacaattt gcaataaata ttcgcccaaa tcggtttcaa ccactttaat gccttctgct
    10261 tctaaaacgt ggttcatccc aatctcttcg gttaccatcg acttcgattt tacgattttc
    10321 ttcgcatttt tagctttcgc cacgtttttg atgtaatcag tggcttcttc ggcggtttcg
    10381 gcaaagaaaa cgtgtccgcc gttttgggtg actttttcgc ttaattggta gagataagcg
    10441 tctaaattgg ctaaaacgtg gttacgaatt gctttcgcat catcacgcca ctcttcccag
    10501 ttgcccagct catccaccat acgctgacgg tttgcaccga ttgtttcctg agccttaact
    10561 aaggctttac gcacaatctc gttattaact tgctgctcaa cacgttgttt aaacggcagg
    10621 gtgttagttt gtaaataaga catcttattt tcctccctgc attaacactt cggcaatgtg
    10681 catcactttg attggcttac cttcacggct taaacgaccg ccaatgttca tcaagcagct
    10741 tacatctgcc ccgattaaat aatccggctc atcggtatct acatttgcga ctttctcttt
    10801 taccatctcg ccggaaattt ctgccatttt gaccgagaat gtgccaccaa aaccacagca
    10861 agtttcttgg tttttgattg gtaataactc taacccttta acgtgctgta ataaagcaat
    10921 aggctcatct ttaatcccca gcttacggaa taagctgcaa gacgggtgat aaactgcttt
    10981 gcccggtaaa taagccccaa catctttcac gcccagtttg ttcacaataa aatcggttaa
    11041 atcatagaaa cgatcagcca ctttcttcgc acgttctgcc cattcttttt cgccaaagcg
    11101 ttcaaaatga tccggatagg ttttgatcgc atatacacaa gaacctgccg gggcgacaat
    11161 cgggtaatca ttagcttcaa aggtttcaac caagtttttc atacctgcta aagcttgctt
    11221 agtgtaaccg ctgttgatcg caggttgtcc acagcaacct tgtttttcta agaaagtgat
    11281 tttacaacct aatttttcca ataaaagcac ggtatttttt gcaacccctg ctttcattac
    11341 atctgcaatg caggttacgt aaaaattaac gttcataaag aactcccaca gtaaatataa
    11401 ataagaatta aactcaaaaa atgccgttct aagacggcga aaggaagagg tggatttgca
    11461 aaaaatttgc taaatctcac cgcttccttt ctaaatcaag taagctgatt attaacttat
    11521 tataggttat agaacatagg tactataaac aatgccgaaa tcgcagcaat aacgccatac
    11581 acgaacattg gcacggctgt tttcttaata atattgcctt cttgtttctc aatgccggta
    11641 acagaacaca cagccacgat gttgttaata cacaccatat tccccattgc accaccaact
    11701 gattgcaatg caagaattaa ggtaacagat aacccgactt gctgtgccgt tgcatattgt
    11761 actgttccga aggttaagtt agataccgta tttgaccctg agaagaacgc accaattgca
    11821 cctaagaacg aggcaaataa tgtccaagat tgaccggtaa attctgcgaa gtttttacca
    11881 atgatctgaa ccattgagtc tacaccacca attaacatta attttaccat cacaatggca
    11941 cctaaaagtg ctaagaatgg ttttttcact tgtttaaatg aagtgccaaa aatctcttga
    12001 gtatttttac ctgataccga gaaaatcggt aagcaaatta atacggtcac aacaaatgga
    12061 atcaacgcag gaacatacag taatttatag ctagttgcta cattcgtacc taaaatattt
    12121 ttcagtgcaa aaattaagcc ttggctaatt tcaaagttag cgaaaccaag cgaactgaca
    12181 atccaagctg ttgcatcatt tagcattgct tttaatggaa gttgctgaat acgggtgatt
    12241 accaaaatga agatcagtaa ccctgttggt aataatgctt ttgccacttc accaaaggtc
    12301 gctttatcgc cgtcaaggtg gttctcgctt ttcgctaaac caatacccat atttgctacc
    12361 cagactgaca ccaataaacc aatcgccccg gcaactaatg atgggaattc gtagttaaat
    12421 tgagcaataa tgaagtaagg aatgacacag gcaaaaatgc tgatataaat aaacacgata
    12481 ttttgacgaa tttctttcca gctcacaatt aaacgcaatg ccataactgg aataataaac
    12541 gcagcaatag agtggatcag tgcagtaata gaaccaattt caagcacttg tgcatcggtt
    12601 agtaaaccat cttttaataa tggtcccata ccaaaccaag ttggtgtgcc aaccgcacca
    12661 aatgacaccg gcacagagtt cattactaag gctaacattg ccacttgtaa cggtttaaag
    12721 cctaaaccaa ccagaattgg agctgcaatt gccgccggcg taccgaaacc tgatgcgcct
    12781 tcaatcataa atgcaaatgc ccaaccgata atcatcaact gtgccaccgg attcgggtta
    12841 attgtcccta accatttacg catagtgttg gttgcacctg aaacttcaga gaaacggtta
    12901 aataaaatcg ccccgaaaat aacggtaatt ggggttaata catcacctaa accggcaata
    12961 atattggcaa agattaacgt ggtatcatta ccaaaatagg taagctgaag cacaaaaatt
    13021 aataacgccg taagtggtaa agccacataa gacggtaacg cgttacgttt taccattaaa
    13081 taaatgagca aaacaattgg aaatacgctc aggaatagag ccatactgca cctctttata
    13141 tgattataaa atgacctaac tggtcggata ggtgcgaatt gtactcttaa aaaaaaacta
    13201 atgtaactag gttgttacaa ttttgtgatc taactcgcat ttttaatgat tgtatggtta
    13261 aaaaagagcc atatttattt tcatttaata cttaatagtt agccctattt aagacatttt
    13321 taccgaatcg agcaatatgc aattaattaa caaaaaaagg ccgcttgcag atcacagatc
    13381 aacaagcggt cttttttagg caaaaatttg caaattaaac attgaaacgg aagtgcatta
    13441 catcgccgtc ttgaacaata taatctttac cttccaaacg ccatttaccc gcttcttttg
    13501 caccggcttc acctttattg tctatgaagt cttggtaagc aattacttcc gcacgaataa
    13561 agcctttctc aaagtcagtg tgaatcacgg ctgctgcttt tggagcggtt gcacctaccg
    13621 aaaccgtcca cgcacgcact tcttttaccc ctgcggtaaa gtaggtttgt aagttaagta
    13681 aacggtagcc tgcacgaata acacggttta agcccggctc ttcaatgcct aaatcttgta
    13741 gaaattccac tttttcttcg tcatcaagct cggcaatttc cgattcaatc gctgcacaca
    13801 ccggtacaac caccgcccct tctttttcgg caatttcacg cacacggtca agataagggt
    13861 tattttcaaa accgtcttcg ttcacgttgg caatatacat tgtcggtttt a
//
LOCUS       DANJWC010000049        13807 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.049, whole genome shotgun sequence.
ACCESSION   DANJWC010000049 DANJWC010000000
VERSION     DANJWC010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.049"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..2864
                     /locus_tag="PWX02_001082"
     CDS             <1..2864
                     /locus_tag="PWX02_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDL1729106.1"
                     /translation="LEKLNNVRFLDSEWKRLLEEYIDKPSENIIEKTRKIHHDHIYDF
                     VFDNGRIQNIYLLDKKEIARNKVQVINQFEQKGSHLNRYDVTILVNGLPLIQIELKKR
                     GVAIREAFNQIHRYSKESFNKENSLFKFLQIFVISNGTDTRYFANTTKRDKNSFDFTM
                     SWAKSDNGLIKDLKDFTATFLQKNTLLNVLVNYSVFDVNDTLLLMRPYQIAATERILW
                     KIKHTHFTNTWNKPESGGYIWHTTGSGKTLTSFKAARLATELDCIEKVFFVVDRKDLD
                     YQTMKEYQRFSPDSVNGSESTAGLKRNLEKQDNKIIVTTIQKLNNLMKSEDNLPIYQQ
                     PVVFIFDECHRSQFGEAQRNLKRKFKKFCQFGFTGTPIFPENALGAETTALVFGTQLH
                     SYVITDAIRDEKVLKFKVDYNDVRPQFRTMEIEKSPEKLTALENKKALLHPERIKEIT
                     QYILAHFKQKTHRLNATGKGFNAMFAVSSVEAAKLYYEQFKIQQQAVQNPLKVATIFS
                     FAANEEQNAIGEIADETFEPTAMNSSAKEFLSLAIDDYNEQFKTNYGVESKEFQNYYR
                     DLAMRVKNQEIDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGLMQAFSRTNRIYDSTK
                     TFGNIVTFRDLEQDTIDAITLFGDKNTRNVVLERSFDEYMQGYEKSSGEYQRGYLVVV
                     KELQTKFPDPTNLEKEEDKKAFTALFGEYLRLENVLQNYDEFHELKNPENIENIVCEP
                     TANYLNHTQIILPERKLQDYRSTYNDIREWQRQQRQGNNTQTSTIDWSDVVFEVDLLK
                     SQEINLDYILELIFEHNKKIKNKPALIEEIRRTIRSSIDNRAKESLVVDFIHQTDLDT
                     IPDKAGVIEAFFQFAQQEQKKEAETLIETENLNVEAAKRYITTSLKREYASENGTDLN
                     DILPKMSPLNPQYKTKKQTVFQKIVAFVEKFKGVGGYLS"
     gene            2948..4897
                     /locus_tag="PWX02_001083"
     CDS             2948..4897
                     /locus_tag="PWX02_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1729107.1"
                     /translation="MALINLSNAYLGFGDHPLLDHTELHIEPGERVCLVGRNGAGKST
                     LMKILAGEVQLDDGKLVFEKDIVVTRLEQDPPRHIEETVFDYVAEGIAHLADLLKQYH
                     HISLQMQTDYSDELLAKLSAVQSQLEHANGWQFENRIQDTLKLLELDPDKRLCELSGG
                     WVRRAALARALVADPDVLLLDEPTNHLDIEAIAWLENLLLDFKGSIIFISHDRAFIRK
                     MATRIVDLDRGKLVSYPSNYDKYLEEKAEDLRVEALQNELFDKKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKALREERRNRREVQGTAKIQIDNTARSGKIVFEVENASHEIGGRTL
                     LKNFSTTILRGDKIALIGANGIGKTTLIKLLLGEIQPTSGSVRCGTKLEVAYFDQYRA
                     DLDTEKTVMDNVADGKQDVEVNGVKRHVLGYLQDFLFPPKRAMTPVKALSGGERNRLL
                     LAKLLLKPNNLLILDEPTNDLDVETLELLEEMLTDYQGTLIIVSHDRQFVDNTVTECY
                     FFEGEGVVNKYVGGYFDAKQQQENYFASQKQSVETPRLASEKSPVKTTESDTKHRVPT
                     EDAKKKVKLSYKEQRELDELPAKMEQLEADIEALQAEVNSAEFFAKDPSYTNAQLQKL
                     ADTEAALEQAFERWEALENIKNGNV"
     gene            4927..5682
                     /gene="tcdA"
                     /locus_tag="PWX02_001084"
     CDS             4927..5682
                     /gene="tcdA"
                     /locus_tag="PWX02_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HDL1729108.1"
                     /translation="MERIDNYEQRFGGIGRLYTPEGLEKLRRSHICVIGIGGVGSWAV
                     EALARSGIGKITMIDMDDICVTNINRQIHAMSGTVAQLKTEAMKERIERINPECVVEI
                     IDDFLTQDNLADYLNRGYDYVIDAIDSLRVKAAMIAFCKRNKIKIITTGGAGGQTDPT
                     KIQITDLSKTVQDPLAARVRSLLRKEYGFSNKNKFGVECVSSTQQLIFPQMGEACEVS
                     ATMNCANGFGAVTMVTATFGFFAVSRVIDKLLK"
     gene            5729..7096
                     /locus_tag="PWX02_001085"
     CDS             5729..7096
                     /locus_tag="PWX02_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HDL1729109.1"
                     /translation="MAIHRVNPKGSMEQLSHLEMELLAKNTQGNLHQLYRNCSLAVLN
                     SGVHTDDSRALLSQYPDFEIRLLTREKGVSLELHNPPETAFVDGKMILNIQYHLFAVL
                     RDIVFVNALKNAIRPLEETSLEALTTNTVFSILRNAKAIEMNTDPNLVVCWGGHSINE
                     TEYQYCRAVGQEFGLRELNIVTGCGAGVMEAPMKGAAIGHANQRYKNGRFIGITEPSI
                     IASEPPNPIVNELVIMPDIEKRLEAFVRLGHAIVVFPGGPGTLEELLYIIGIKLNPAN
                     HAQQLPLILTAPKESADYFYAIDQFIGETLGEEARSLYEIIIDDPVLVARKVKQAMIE
                     VKSSRYESGDAYNFNWSLKIEEDFITPFIPTHENMANLNLSLAQPKEQLAANLRRAFS
                     GIVAGNIKPETSDLIEKYGVFKLHGDRKLMEHIDKLLERFVVQHRMKIPTGEAYQPCY
                     EIVKG"
     gene            7116..8663
                     /locus_tag="PWX02_001086"
     CDS             7116..8663
                     /locus_tag="PWX02_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HDL1729110.1"
                     /translation="MYLKHIEISGFRGINHLSLQLRPNMVLIGENAWGKSSLIDALSL
                     IFNLEQALYQFQFKDFHIQYTDTPNQVRDIQLVFTFCEEHQHDLAVQFPAYLAQAAFY
                     IDNHRCVSLAVTGKLHGEQISTDYQFVDQSLKPIQIEQAENAIRQFIYHHPVYRLRDA
                     RLNNRVHNELLLKGSSQLEDDFQRELNALSSLLKYYFLTSQSRYLIKTSMQDTALWWE
                     KVKSLCLRLKQQPELVESAQQHISTLFLFNPRSTVIEKPIILFEDLNAQLHPRMIAIF
                     WELLGFLPSQRITTTNSIELLSQVPLREICRLVRHHDHTQAYWLERHSLGKEDLRKLT
                     FHIHHNRGLALFAKAWILVEGETEVWILQELARLLDIHLEMEGIKIVEFAQCGLRPLI
                     KYARAMGIEWYVLTDGDDSGKRYADTVKSMEEDFAHRLTMLPQKDIEHFFYKAGLRNV
                     FIQLANWRSQEKYPVSYIIKRAIQRSSKPDLAIALANEMEQRGEHSIPTLFKQLFADV
                     LALINKV"
     gene            8886..9920
                     /gene="metN"
                     /locus_tag="PWX02_001087"
     CDS             8886..9920
                     /gene="metN"
                     /locus_tag="PWX02_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HDL1729111.1"
                     /translation="MIQLKNISKQFEVKGKKITALDNVNLEVPRGTIYGVIGASGAGK
                     STLIRCVNLLERPTIGQVIVDGQDLTAMSDKELIHARRNIAMIFQHFNLLSSQTVFEN
                     VALSLRLSNTPKEQVEKKVNELLDLVGLTDRKDVYPANLSGGQKQRVAIARALASDPH
                     VLLCDEATSALDPATTQSILQLLKDINKRLGLTILLITHEMEVVKRICDRVAVIDKGQ
                     LIESGSVSEIFSNPKTELAQKFIQSTFHFELPQEYTEQLSSSPTEHSKPIIKFEFTGR
                     SVDAPLLSMASKKFGIDFSILMSQIDYAGGVKFGFVIAEVSGNPDSIAEAKFYLIDNN
                     VKVEVLGYVG"
     gene            9910..10587
                     /locus_tag="PWX02_001088"
     CDS             9910..10587
                     /locus_tag="PWX02_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1729112.1"
                     /translation="MWADFMQEMTPQMWSLVWESTFETVYMGFVATALAVVIGLPIGF
                     LAFLTGKGEILENPRLHQVLDVVINIGRSIPFIILLIILLPFTRLIVGTTLGTTAAIV
                     PLSVSAIPFFARLTSNALLEIPSGLTEAAKSMGATNWQVVTKFYLPESLPILINGITL
                     TLVALIGYSAMAGAVGGGGLGNLAISYGEHRNMVYVKWIATIIIVAIVMISQKLGDHL
                     AKRYDHR"
     gene            10610..11443
                     /locus_tag="PWX02_001089"
     CDS             10610..11443
                     /locus_tag="PWX02_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family lipoprotein"
                     /protein_id="HDL1729113.1"
                     /translation="MSFKKILGVALVSALALTACKEEKKAESTAAPAAQAPAKIKVGV
                     MSGPEHTVAERAAQIAKEKYGLEVEFVLFNDYALPNTAVSKGDLDANAFQHKPYLDKD
                     SQSKGLNNLVIVGNTFVYPLAGYSKKVKNVSELAEGAVIAVPNDPSNLARALILLEKQ
                     GLIKLKDNTNLFSTSVDIIENPKNLKIKEVDTSIAAKALDDVDLAVVNNTYAGQVGLN
                     TQDHGVFVESKDSPYVNIIVARQDNKDAANVQNFIKSYQTEEVYQEAQKHFKDGVVKG
                     W"
     gene            11526..12356
                     /locus_tag="PWX02_001090"
     CDS             11526..12356
                     /locus_tag="PWX02_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL1729114.1"
                     /translation="MNFKKLLGVALVSALALTACKDEKAQAPATTAKTENKAPLKVGV
                     MTGPEAQMTEVVVKIAKEKYGLDVELVQFTEYTQPNAALHSKDLDANAFQTVPYLEQE
                     VKDRGYKLAIIGNTLVWPIAAYSKKIKNISELKDGATVAIPNNASNTARALLLLQAHG
                     LLKLKDPKNVFATENDIIENPKNIKIVQADTSLLTRMLDDVELAVINNTYAGQAGLSP
                     DKDGIIVESKDSPYVNLVVSREDNKDDPRLQTFVKSFQTEEVFQEALKLFNGGVVKGW
                     "
     gene            12408..13199
                     /gene="metQ"
                     /locus_tag="PWX02_001091"
     CDS             12408..13199
                     /gene="metQ"
                     /locus_tag="PWX02_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HDL1729115.1"
                     /translation="MKIMKLAGAVAIFSLFLTACNDKAEKLKVGVISGPEHKVMEVAA
                     KIAKEKYNRDVELVVFTDYATPNAALDKGDLDLNAFQHKPYLDNQIQEKGYKLVPVGN
                     TFVYPIAAYSKKIKSLAELKDGDTIAVPNDPTNLARALILLEKQGLIKLRADAGLKAT
                     SVDIIENPRKLVIQEIEAPLLPRTLDDVAFSIINTTYAGQNGLTPTKDGIFVEDKDSP
                     YVNLIVARENNQHSEAVKDLVKAYQTEEVYNKANEEFKGAMIKGW"
     gene            complement(13243..>13807)
                     /gene="metK"
                     /locus_tag="PWX02_001092"
     CDS             complement(13243..>13807)
                     /gene="metK"
                     /locus_tag="PWX02_001092"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDL1729116.1"
                     /translation="KELIEGVMEEIIKPVLPSEWLSQNTKYFINPTGRFVIGGPMGDC
                     GLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQ
                     LSYAIGVAAPTSIMVETFGTGKVSNEILVKLIYQNFDLRPYGLIKMLDLIKPIYRETA
                     AYGHFGRKHFPWEKTDKAEALRAGAGL"
BASE COUNT         4310 a         2522 c         3137 g         3838 t
ORIGIN      
        1 agttggaaaa attaaataat gtgcggtttt tagattcaga gtggaaacga ttattagaag
       61 aatatattga taaaccgtct gaaaatatta ttgagaaaac cagaaagatc catcacgacc
      121 atatttatga ttttgtgttt gataatgggc ggattcaaaa tatctatttg ttggataaaa
      181 aagaaattgc tcgtaataaa gtacaggtta tcaatcaatt tgagcaaaaa ggttctcatc
      241 ttaatcgcta tgatgtaacg attttggtca atggcttgcc attaatacaa attgaattga
      301 aaaagcgagg cgtggcaatt cgggaagctt ttaatcaaat tcatcgttat agtaaagaga
      361 gttttaataa agaaaattct ctgtttaagt ttttgcagat ttttgtcatt tctaacggca
      421 cagatacccg ttattttgcc aatacgacca agcgggataa aaacagcttt gattttacaa
      481 tgagttgggc aaaatcggat aatggcttaa ttaaagatct aaaagatttt accgctacct
      541 ttttacagaa aaatacctta ttaaatgtgc tagtgaatta ttcggtattt gatgtaaatg
      601 acacgttatt attgatgcga ccttatcaaa ttgcggcaac cgagcgtatt ttatggaaaa
      661 taaaacatac gcattttacc aatacttgga ataaaccgga gagtggtggt tatatttggc
      721 atactaccgg ttcgggtaaa acgctgacca gttttaaagc ggctcgattg gcaaccgaat
      781 tagattgtat tgagaaagtc ttttttgtgg tggacagaaa agatctcgat tatcaaacaa
      841 tgaaagagta tcaacgtttt tcaccggata gcgtaaacgg ttcggaaagt acagccggtt
      901 tgaagcgtaa tcttgaaaag caagataata aaattattgt taccactatt caaaaattaa
      961 ataatttaat gaagagtgaa gataatttac caatatatca acagccggta gtgtttattt
     1021 ttgatgagtg tcatcgtagc caatttggtg aagctcaacg taatttaaaa cgaaaattta
     1081 aaaaattttg tcaattcggt tttaccggca cccctatttt ccctgaaaat gcattagggg
     1141 cagaaacaac cgcattggta tttggtacac agctacactc ttatgtgatt accgatgcga
     1201 ttcgtgatga aaaagtgctg aaatttaagg tcgattataa tgatgttcgc ccacaattta
     1261 ggacaatgga aattgaaaaa agtcctgaaa aactcaccgc acttgagaat aaaaaagcct
     1321 tattgcaccc tgagcggatt aaagaaatta cccaatatat tttggcacat tttaaacaaa
     1381 aaacgcaccg tttgaatgcc acaggcaaag gctttaatgc aatgtttgcc gtcagttctg
     1441 tggaagcggc aaaactctat tatgagcagt ttaaaattca gcagcaagcg gtgcaaaatc
     1501 cgttaaaagt tgcaacgatt ttttcttttg ctgccaatga agagcaaaat gcgattggtg
     1561 aaattgccga tgaaaccttt gaacctaccg caatgaatag ttcggcaaaa gaatttttga
     1621 gtttggcgat tgatgattat aacgagcaat ttaaaacaaa ttatggtgta gaaagtaaag
     1681 aatttcaaaa ttattaccgt gatttagcaa tgcgggtgaa aaatcaggaa attgatttat
     1741 tgattgtagt aggaatgttt ttaacaggct ttgatgctcc aacgttaaat accttatttg
     1801 tggataaaaa tctgcgttat cacgggttga tgcaagcctt ttcccgcact aaccgcattt
     1861 atgactctac taaaactttc ggcaatatcg tgactttccg agatttggaa caggatacga
     1921 ttgatgcgat tacgctattt ggcgataaaa atacccgtaa tgtggtgtta gaacgtagtt
     1981 tcgatgaata tatgcagggc tatgaaaaga gcagtggtga atatcaacgt ggttatttgg
     2041 tggtagttaa agaattacaa actaaatttc ctgatccaac gaatcttgaa aaagaggagg
     2101 ataagaaagc ttttactgca ttatttggtg aatatttacg tttggaaaat gtattacaga
     2161 attatgatga gtttcacgaa ctgaaaaatc ctgaaaatat agagaatata gtttgtgagc
     2221 cgactgcaaa ttatcttaat cacactcaga ttattttgcc tgaacgaaaa ttgcaagatt
     2281 atcgttcaac ctataacgat attcgggaat ggcaacgcca acaacgacaa gggaataata
     2341 ctcaaacttc aacaattgat tggagtgatg tggtgtttga agtggattta ctaaaatccc
     2401 aagagatcaa tttagattat attttagagc ttatttttga acacaataag aaaatcaaaa
     2461 ataagcctgc gttaattgaa gaaattcgcc gtactattcg ttcaagcatt gataatcgag
     2521 cgaaagaaag tttggtagtg gattttattc accaaaccga tttggatact attcctgata
     2581 aagccggtgt cattgaggca ttcttccaat tcgcccagca agaacagaaa aaagaggcag
     2641 aaaccttaat tgaaaccgaa aatttaaatg tagaggctgc aaaacgttat atcaccactt
     2701 cactaaaaag ggaatatgcc agcgaaaacg gcacggattt aaatgacatt ctgccgaaaa
     2761 tgagcccact taatccgcaa tataaaacta aaaaacagac ggttttccaa aaaatcgtgg
     2821 cgtttgtgga gaagtttaag ggagttggtg ggtatttatc ttaacaagaa taaaaacgag
     2881 tagaacagaa cgctttcata caagcggtta gagaattgct aaaatttaca attttaacga
     2941 gaatactatg gcacttatca atctttccaa cgcctattta ggctttggcg atcatccttt
     3001 attggatcat actgaattac atatcgaacc cggcgagcgg gtttgtttgg tggggcgtaa
     3061 cggggcgggt aaatcgaccc tgatgaaaat tctggcgggc gaagttcagc ttgatgacgg
     3121 caagttggta tttgaaaaag acattgtcgt tacacgcctt gaacaagatc cgccccgcca
     3181 tattgaagaa acggtgttcg attatgtggc agaaggcatt gcccatcttg ccgacttatt
     3241 gaagcaatat caccacattt ccctgcaaat gcagaccgat tacagcgatg aactgctcgc
     3301 caaactttca gcggtgcagt cgcaattaga acacgctaac ggttggcagt ttgaaaaccg
     3361 cattcaagac accttgaaac tgttggaact cgaccccgac aaacgcctgt gcgaactctc
     3421 gggtggttgg gtacgccgtg cggcattggc tcgggcgttg gttgccgatc ctgatgtatt
     3481 gctgttggac gagccgacca accacctcga catcgaggcg attgcgtggc tggaaaatct
     3541 gttgttggat ttcaaaggca gcattatttt tatctcgcac gaccgtgcct ttatccgcaa
     3601 aatggcgact cggattgtgg acttggatcg aggcaaactg gtttcctacc caagcaatta
     3661 cgataaatac ttggaagaaa aagccgaaga tctgcgagtg gaagcgttgc aaaatgagct
     3721 gttcgacaaa aaactcgccc aagaagaggt ctggatccgt caagggatta aggcaagacg
     3781 cacccgaaac gaagggcgtg tgcgggcgtt gaaagcgttg cgtgaagagc gtagaaaccg
     3841 ccgagaggtg caaggcacgg cgaaaattca gatcgacaat actgcacgca gcggcaaaat
     3901 tgtgtttgaa gtggaaaatg ccagccatga aatcggcggc agaaccctgc tgaaaaattt
     3961 cagcaccacc attttgcgtg gcgataaaat tgccttgatt ggggcgaatg ggatcggcaa
     4021 aaccacgctg attaagctgt tacttggcga aattcagccg acatcaggtt cggtgcgttg
     4081 tggcacaaaa ttggaagtgg cgtactttga ccaataccga gccgatcttg acactgaaaa
     4141 aaccgtgatg gacaacgtgg cagatggcaa gcaagatgta gaagtaaacg gggtgaaacg
     4201 ccacgttctt ggctatctgc aagacttctt gttcccgccc aaacgggcaa tgacaccggt
     4261 gaaagccctt tcgggtggtg aacgcaaccg cttgttgctg gcaaaactgt tactcaaacc
     4321 gaacaacttg ctgattttgg acgaaccgac caacgatttg gacgtggaaa ccctcgaact
     4381 gctggaagag atgctcaccg actatcaagg caccttgatt attgtcagcc acgaccgtca
     4441 atttgtggat aataccgtaa ccgaatgcta cttcttcgag ggcgaaggcg tggtgaataa
     4501 atatgtcggc ggctatttcg atgccaaaca acagcaggaa aattactttg cctcccaaaa
     4561 gcagagcgta gagacgccac gcttggcgtc cgaaaaatcg cctgtaaaga ctactgaatc
     4621 ggacacgaag catcgtgtcc ctacggaaga tgcgaagaaa aaagttaaac tttcctacaa
     4681 agaacagcgt gaactggacg agttaccggc taaaatggaa cagctcgaag ccgatattga
     4741 agccttgcaa gccgaagtaa acagtgccga attcttcgcc aaagatccaa gctacaccaa
     4801 tgcccagcta caaaaacttg ccgacaccga agccgcgtta gaacaagcct ttgaacggtg
     4861 ggaagcgttg gaaaatatta aaaatgggaa tgtgtaagcg gtcattttta cggaaaattt
     4921 tacaaaatgg aacgaattga taactacgaa cagcgttttg gtggcattgg tcgcctttat
     4981 acgccggaag gattggagaa actacgccgt tcgcatattt gcgtgattgg gattggcggg
     5041 gttggctcgt gggcagtgga ggcattggct cgctcaggta tcggtaagat cacgatgatc
     5101 gatatggacg atatttgcgt gaccaatatc aaccgccaaa ttcacgcaat gagtggcacg
     5161 gtggctcaac tgaaaaccga agcgatgaaa gagcggattg agcggatcaa ccctgaatgt
     5221 gtggtggaaa ttattgatga tttcttaacg caggataatc tcgcagacta cctcaatcga
     5281 ggatatgact atgtgatcga tgcgattgac agtttgcggg taaaagcggc gatgattgcg
     5341 ttttgtaaac gcaataagat caaaattatc accaccggcg gggcgggcgg gcagaccgat
     5401 ccgaccaaaa ttcaaattac cgatttaagc aaaacggtgc aagatccgtt ggcggcaagg
     5461 gttcgtagcc tgttgcgtaa agagtacggt ttcagcaata aaaacaaatt cggcgtggag
     5521 tgcgtttctt cgactcagca actgattttc ccacaaatgg gtgaggcctg tgaggtgtcg
     5581 gcaacaatga actgtgccaa cggctttgga gcggtgacaa tggtgacggc aacgttcggc
     5641 ttttttgcgg tgagtcgggt gattgataaa ctattaaaat aacaagcggt catattaaat
     5701 gaattttttg caaatcaagg agtaaataat ggcaatacat cgagtcaacc cgaaagggag
     5761 tatggaacag ctttcccatt tggaaatgga gttgttggca aagaatacgc agggtaattt
     5821 gcatcaactt tatcgtaatt gctctttggc ggtgctaaat tccggtgtgc ataccgatga
     5881 cagccgagcg ttgttaagcc aataccctga ttttgaaatc cgcttattaa cccgtgaaaa
     5941 aggcgtgtcg cttgaattgc ataatccacc cgaaactgcg tttgttgacg gcaagatgat
     6001 tttgaatatt caatatcacc tttttgcggt gctgcgggat attgtgtttg tgaatgcgtt
     6061 aaaaaatgcg attagaccgc ttgaagagac ttctttagaa gccttaacca cgaataccgt
     6121 attttcgatt ttgcggaatg cgaaagcgat tgaaatgaat accgacccaa atttggtggt
     6181 gtgctgggga gggcattcga ttaatgaaac cgaataccaa tattgccgtg ccgtcgggca
     6241 agagtttggc ttgcgtgagc tgaatattgt gaccggttgc ggagccggtg tgatggaagc
     6301 cccgatgaaa ggagcggcta tcggacacgc caatcaacgt tataaaaacg ggcgttttat
     6361 cggcattacc gagccgtcta ttattgcctc cgagccgcca aacccgattg tgaatgaatt
     6421 ggtaattatg ccggatattg aaaaacggtt ggaagccttt gtgcgtttag ggcacgccat
     6481 tgtggttttc ccgggcgggc cgggaacgct ggaagagttg ctgtatatta tcggtattaa
     6541 gttaaacccg gctaaccatg ctcagcaatt accactgatt ttaaccgccc cgaaagaatc
     6601 ggcagactat ttctatgcca tcgatcaatt tattggcgaa actttaggcg aagaagctcg
     6661 cagtttgtat gaaattatta ttgatgaccc ggtgttggtg gctcgtaaag tcaagcaagc
     6721 gatgattgag gtaaaaagca gccgttatga aagtggtgat gcttataatt tcaactggtc
     6781 gttaaaaatt gaggaagatt ttattacgcc atttattcca acccacgaaa atatggcaaa
     6841 cctgaattta agtttagcac agccaaaaga gcagctggct gcgaatttaa gacgggcatt
     6901 ttcagggatt gttgcaggta atattaagcc ggagacctcc gatttaattg agaaatatgg
     6961 ggtgtttaaa ttacacggcg atcgtaaatt aatggagcat atagataaac tgttggagcg
     7021 ttttgtggtt caacaccgta tgaaaattcc aacgggtgag gcgtaccaac cttgctatga
     7081 aattgtaaaa gggtaatgct aacaaggggt aaggtatgta tttaaagcat attgaaatca
     7141 gtggctttcg tggtattaac catttatcgc tccagctacg accaaatatg gtattgattg
     7201 gcgaaaatgc gtgggggaaa tccagtctga ttgatgcatt aagcctgatt tttaaccttg
     7261 agcaggcgtt gtatcaattt cagttcaaag atttccatat tcaatacacc gataccccca
     7321 accaagtgcg ggacattcaa ctggtgttta ccttttgtga agagcatcag cacgatttag
     7381 cggtgcaatt tcccgcttat ttggcacaag ccgcgtttta tattgataat caccgctgtg
     7441 tttcgcttgc cgtaaccgga aaattacacg gagagcagat tagcaccgac tatcaatttg
     7501 tggatcaaag cctaaaaccg atccagatag aacaagcgga aaacgccatt cggcagttta
     7561 tttaccatca ccctgtttat cgcctgcgtg atgcaaggct caataacagg gtgcataatg
     7621 aattattact taaaggatcg tctcaacttg aagacgattt tcaacgtgaa ttaaacgccc
     7681 tctcttcgct actgaaatac tattttctaa ccagccaaag tcgctatttg atcaaaacca
     7741 gtatgcaaga caccgcattg tggtgggaaa aagtaaaatc cctctgttta cgcctaaaac
     7801 agcagccgga attggtcgaa tctgctcagc agcatatttc tactctattt ttgtttaatc
     7861 ctagatctac agtaatagaa aagccgatta ttttatttga agatctgaat gctcaactac
     7921 accctcgaat gattgcgatt ttttgggaac tgctcggttt tttacccagc caacggatca
     7981 ccactactaa ttctattgaa ttgttatcgc aagtgcctct gcgtgaaatc tgccgtttgg
     8041 tgcgtcatca cgatcatacc caagcctatt ggcttgaacg ccacagttta ggcaaggaag
     8101 atttgcgaaa attaaccttt catattcacc ataatcgtgg cttggcactg tttgctaaag
     8161 cgtggatttt ggttgagggg gaaacggagg tttggatttt gcaagagctg gcgagattat
     8221 tagatattca tttggaaatg gaagggatca aaatcgttga atttgctcag tgtggcttac
     8281 gtccgttaat taaatacgcc agagcaatgg ggatcgaatg gtatgtgctg actgacggtg
     8341 acgattccgg caaacgttat gcagatacgg taaaaagtat ggaagaggat tttgctcacc
     8401 ggctcacgat gttgccacaa aaagatatcg aacatttttt ctacaaagca ggcttgcgga
     8461 atgtgtttat acagcttgca aattggcgtt cacaagaaaa atatccggtg tcctacatca
     8521 ttaaacgagc gattcaacga agctcaaagc ctgatttagc cattgcatta gccaatgaga
     8581 tggagcaacg gggagagcat tcaattccaa ctttatttaa acaattattt gcagatgtgc
     8641 tggcattgat aaacaaagta tagaatacaa gcggttaaat ttgacaaaaa attgccaatt
     8701 ttgaccgctt gtttgcttgt gatgaaaacc aatcatacaa aactctaatt tatttcaagt
     8761 gcaagctatt attttatgcg ttatgcttat ttggaataaa aaaatcgggt tagttgattg
     8821 acatagcatt ctagacgtct aaaataacca aaaataatag ttaattgaat gtggataaat
     8881 agaaaatgat tcagctgaaa aatatcagca aacaatttga ggtaaagggc aaaaaaatta
     8941 ccgctctgga taatgtaaac ctagaagtac caagaggcac tatttatggt gtcattgggg
     9001 catcaggggc aggaaaaagt accttaattc gctgtgtaaa tttattagaa cgcccaacaa
     9061 tcgggcaagt gattgtcgat gggcaagatt taacggcaat gtcggataaa gagttgattc
     9121 acgctcgccg taatattgca atgattttcc aacattttaa tttgctttca tcacaaactg
     9181 tatttgaaaa cgtggcgtta tcgttgcgtt taagcaatac gccaaaagag caagttgaga
     9241 agaaagtcaa tgagctgttg gatttagtgg ggttaacaga tcgtaaagat gtttaccctg
     9301 caaatctttc cggtgggcag aaacagcgtg tagcgattgc tcgtgcgtta gccagcgatc
     9361 cacacgtttt gttgtgtgat gaagcgacca gtgcgttaga tccggcgact acgcaatcca
     9421 ttttgcagct attaaaagat attaataaac gtcttggttt gacgatttta ttaatcactc
     9481 acgaaatgga agtggtaaaa cgcatttgtg accgagtagc agtgattgat aaagggcagt
     9541 tgattgaatc tggctcggta agtgaaattt tctcgaatcc gaaaaccgaa ttagctcaga
     9601 aatttattca atcgactttc cattttgaat taccgcaaga atataccgag cagctttcaa
     9661 gttcgccgac tgaacatagt aagccgatta ttaaatttga gtttaccgga cgttcggtgg
     9721 atgcaccttt gctttcaatg gcatcgaaga aattcggcat tgattttagt attttgatgt
     9781 cgcaaattga ttatgcaggc ggggtcaaat ttggatttgt aattgcagaa gtgagtggta
     9841 atccggattc cattgcagaa gcaaaatttt atttaattga taacaatgta aaagtagagg
     9901 tgttaggcta tgtgggctga ttttatgcag gagatgacgc cacagatgtg gagcttagta
     9961 tgggaatcta cttttgaaac cgtttatatg ggctttgtgg caacggcatt ggcggtagtc
    10021 atcggtttac cgattggttt cttggcgttt ttaaccggta aaggcgaaat tttagaaaat
    10081 ccgagattac accaagtgtt agatgtcgtt attaacatcg ggcgttcgat tccgttcatt
    10141 attttattga ttattttgtt gccatttact cgcttaattg tgggcacaac attaggcaca
    10201 acggcagcga ttgttccttt aagtgtttcg gccattccgt tttttgcccg tttaacttca
    10261 aatgcgttgc ttgaaattcc gtccggttta acagaagcgg caaaatcaat gggagcaaca
    10321 aattggcagg tagtgacaaa attttattta ccggagtcat taccgatttt gattaatggc
    10381 attacgctaa cgctggttgc tttaattggc tactcagcaa tggcgggggc tgttggtggc
    10441 ggcggtttag gcaatttagc gattagctat ggcgaacacc gtaatatggt gtatgtgaaa
    10501 tggattgcta ccattattat tgttgcgatt gtgatgatta gccaaaaatt gggtgatcat
    10561 ttagcaaaac gttacgatca tcgttaattt tatttaaaaa gaggtttcta tgagtttcaa
    10621 gaaaatttta ggcgttgcat tggtttctgc attagcatta accgcttgta aagaagagaa
    10681 aaaggcagaa tctaccgctg ctccggcagc tcaagctccg gctaaaatca aagtaggggt
    10741 aatgtcaggc cctgagcata ccgttgcaga acgtgcagca cagattgcaa aagaaaaata
    10801 tggtttagaa gttgagttcg ttttatttaa cgactacgct ttaccgaata ctgcagtaag
    10861 taaaggtgat ttagatgcaa acgcattcca acacaaacca tatttagata aagacagcca
    10921 atcaaaaggt ttaaacaact tagtgattgt gggtaacacc tttgtttatc cgttagcggg
    10981 ctactctaaa aaagtgaaaa atgtgtctga gttagcagaa ggtgcggtaa ttgcagtacc
    11041 aaatgatcct tcaaacttag ctcgtgcttt gattttatta gaaaaacagg ggttaattaa
    11101 attaaaagat aataccaatt tattctcaac ttcagtggat attatcgaaa atccgaaaaa
    11161 cttaaaaatt aaagaagtgg atacttcaat tgccgcaaaa gcgttagatg acgtagattt
    11221 agcggtggta aataacactt atgccgggca ggtaggctta aatacccaag atcacggtgt
    11281 atttgttgag tctaaagatt caccgtatgt gaatattatt gtggctcgcc aagacaataa
    11341 agatgcagct aatgtacaaa actttattaa atcttaccaa accgaagaag tgtaccaaga
    11401 agcacaaaaa cactttaaag atggtgtagt aaaaggttgg taatttagct gatttttact
    11461 taaatggcga ttaagcgcta ggttaatcgc cattttcatc tttaaaatat aggaaggagc
    11521 aaacaatgaa ctttaaaaaa ttattaggtg tggcgttagt atctgcctta gcacttactg
    11581 cgtgtaaaga tgaaaaagca caagcacctg ctacaacagc taaaactgaa aacaaagccc
    11641 cattaaaagt gggtgtgatg accggccctg aagcccaaat gaccgaagtg gtagtgaaaa
    11701 ttgcaaaaga gaaatatggc ttagatgtag agttagtgca gtttactgaa tacactcaac
    11761 caaatgccgc acttcattct aaagatttag atgctaacgc gttccaaacg gtgccttatt
    11821 tagagcaaga agtgaaagat cgtggttata aattagcgat tatcggcaat acgctagtat
    11881 ggccaatcgc ggcttattct aaaaaaatta aaaacatttc cgagttaaaa gacggagcga
    11941 ctgttgcgat tccaaacaat gcaagtaata ctgctcgtgc gttattattg cttcaagctc
    12001 acggtttatt gaaattaaaa gatccgaaaa atgtgtttgc taccgaaaac gatattatcg
    12061 aaaacccgaa aaatatcaaa atcgtacagg cggatacctc acttttaacc cgtatgttag
    12121 atgatgtaga acttgcggta atcaacaaca cttacgcagg tcaagctggg ttaagtccgg
    12181 ataaagacgg tattattgtg gaatctaaag attcaccgta tgtgaattta gtggtaagtc
    12241 gtgaagataa taaagatgac ccacgcttac aaacttttgt gaagtcattc caaaccgaag
    12301 aagtattcca agaagcgtta aaattattta acggtggtgt ggtgaaaggt tggtaatcag
    12361 ttggacggca tattctgccg tccttttctt aaaaggagaa acagataatg aaaataatga
    12421 aattagccgg tgcagttgca attttctcgc tttttttaac cgcttgtaat gataaagccg
    12481 aaaagttgaa agtcggtgtg atttccggcc ctgaacataa ggtaatggaa gtggcggcaa
    12541 aaattgcaaa ggaaaaatat aaccgtgatg ttgaattagt ggtatttacc gattatgcca
    12601 cgcctaatgc agctttagat aaaggcgatc ttgatttgaa tgctttccag cataaacctt
    12661 atttagataa ccaaattcag gaaaaaggct ataaattagt gccggtcggc aatacttttg
    12721 tttatccgat tgcggcttat tccaaaaaaa ttaaatcgct ggcagagttg aaagatggtg
    12781 atactattgc agtgcctaat gatccgacta atttagcccg tgctttaatt ttattggaaa
    12841 aacaaggttt aattaagctg cgagcagatg caggcttaaa agcaaccagt gtggatatta
    12901 ttgaaaaccc tcgtaaattg gtgatccaag aaattgaagc accattattg cctcgaacgt
    12961 tggacgatgt tgccttttcg attattaata ctacctacgc agggcaaaac ggtttaacgc
    13021 caaccaaaga cggaatattc gttgaagaca aggattcgcc ttatgtgaat ttaattgttg
    13081 cccgtgaaaa taaccaacat tctgaagccg taaaagattt ggtgaaagcc taccaaacag
    13141 aagaggtgta taacaaagcg aatgaagagt ttaaaggggc gatgataaaa ggctggtaat
    13201 tgaagataaa taaggcatag attcctatgc cttacttttt tgttacagac ctgctccggc
    13261 tcttaatgct tcggctttgt cagttttttc ccaaggaaaa tgtttacgac cgaagtgtcc
    13321 gtaggcagcg gtttcacggt agattggttt gattaaatct aacattttga ttaagccata
    13381 cgggcgtaag tcaaagtttt ggtagattaa tttcacaagt atttcgttgc tcactttacc
    13441 tgtgccgaag gtttcgacca taattgatgt tggggcagcc accccgatag cataagaaag
    13501 ttggatctca cagcgatcgg ctaagcctgc ggcaacgata ttttttgcga cataacgagc
    13561 agcataagca gcagagcgat ccacttttga cgggtcttta ccagagaatg caccaccacc
    13621 gtggcgagcc gcaccaccgt aggtatctac aatgatttta cgcccggtta aaccacagtc
    13681 acccattgga ccgccaatca caaaacgacc ggttgggttg atgaaatatt tggtgttttg
    13741 gctgagccat tcgcttggta gtaccggttt gatgatctct tccatcaccc cttcaattaa
    13801 ctctttt
//
LOCUS       DANJWC010000050        13729 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.050, whole genome shotgun sequence.
ACCESSION   DANJWC010000050 DANJWC010000000
VERSION     DANJWC010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13729
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.050"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            199..1728
                     /gene="mepM"
                     /locus_tag="PWX02_001093"
     CDS             199..1728
                     /gene="mepM"
                     /locus_tag="PWX02_001093"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HDL1729117.1"
                     /translation="MQHVILARDRKRHKKHFKIAVFLLAVFSLFVGIVLALKDQSSIN
                     NTSSQEDIIAIDKQEKYQSINTNLAENNTLHQEQHSSFINYVTDLLTQSALVIEHDDE
                     IVDDQTATSYEDELVGQDDEVEEGIETVENQVPGEKLPEEAEKALEDLLDVADQALRI
                     QNQFSYTVARGDKLKDVLEQSGLTASVAKNLIKQHSALAQLEPGQQFYWVLDNDGELE
                     YMNWLVSEKEERVYERKENGKFSFQRFEKKGEWKLDVVRGTIQNSFAVSLKAVGLSDR
                     QINQLAVGLQSQIATNKLKKGDRFAILIKREYIDGKVTDLGNVEGIHVISSKKSYYAI
                     QADNGRYYSRYGETLSGGFARHPLLFAARVTSSFNPKRRHPITGRISPHKGVDYGVPI
                     GTPIIAPSDGVVEHVAYQARGAGRYIRIKHGHITTVYMHLSKTLVKPGQSVKKGERIA
                     LSGNTGGSTGPHLHYEFHINGRPVNPITVKLPGSNSGMGNKERQAFLNKAKNVESRLK
                     M"
     gene            1738..2421
                     /gene="dsbC"
                     /locus_tag="PWX02_001094"
     CDS             1738..2421
                     /gene="dsbC"
                     /locus_tag="PWX02_001094"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HDL1729118.1"
                     /translation="MKKNLITLGLMCLSTITFASDAKLKSQLEKIGATDVKISDSPLT
                     GFKMATSNEGVVYISQDGRYVIQGKVFELKNGKAIDITNQGLLAELNSYTNEMIVYPA
                     KNEKHVITVFMDITCHYCHLLHTKIKEYNDLGITIRYLAFPRGGMNTQTAKQMEAIWT
                     SADKVKALNDAEDGKLPTQIKTPDIVKKHYQLGIKFGVTGTPNMVTSEGELIGGYVEP
                     KELAKMLSE"
     gene            2506..4227
                     /gene="recJ"
                     /locus_tag="PWX02_001095"
     CDS             2506..4227
                     /gene="recJ"
                     /locus_tag="PWX02_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDL1729119.1"
                     /translation="MEKIIKRRPLLTTEIVSTNPLLNRIYQSRGIKNTQELEHNLKNL
                     HRPQQLANIEQAVDLLLQAFREKSRIIIVGDFDADGATSTSLALIALRQLGFDKVDYL
                     IPDRFSQGYGLSVAVAEMVLAKGADLVLTVDNGISSTEGIALLKSHQIQVLVTDHHLP
                     PESLPNADALVNPNLAFCEFPSKSLAGVGVIFYVMMALRSRMREQHLFENKPEPNFAE
                     LLDLVALGTVADVVPLDQNNRILVHQGLQRIRSGHCRLGIKALAEVGKRDLSTLQAAD
                     LGFAIAPRLNAAGRLENMSLGVELLICENMELARRLALDLDSLNQTRKEFEQEMKAEA
                     LSICANLPSLAQSEQAHGIVLYQADWHQGVIGILASRIKDQFNRPVIAFAQESEESEY
                     LKGSARSINGVHMRDLLEHIDMRHPDLIEKFGGHAMAAGLTIHQSKLDLFKQIFDQSI
                     NAIIEPEQLQGIIYTDGELNAQEFTLANAEMIRQGGPWGQHFPEPSFEGEFSILQQKL
                     LAGKHLKLMLQSEKGQLFDAIWFNVDVRAFPDLSIKKARLVYRLDINEFRGEKSLQLK
                     VEYLKYI"
     gene            4396..5202
                     /locus_tag="PWX02_001096"
     CDS             4396..5202
                     /locus_tag="PWX02_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005726415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDL1729120.1"
                     /translation="MALAVAACGNLSKVSDEGTTENPVFPKISESEFNHDGSQFGSWP
                     NWENVRQIEKGMNKDQLYNLIGRLHFEEGLYGVREWDYAFNYRENGVHKICQFKILFD
                     KNMNAQSFFWYPNGCNGNSSFTLTGDFLFDFDKATLTTKGKEVVDNVAQQLKASKAQQ
                     VKVAGYTDRLGSAAYNLDLSQRRSNTVKARLVQQGVTAQIEAVGYGKANQVKACDGET
                     GQELKDCLRPNRRVEISANGGVMKQNEGGNVAGPNGPAPLYQTPAYDGSK"
     gene            5290..6108
                     /locus_tag="PWX02_001097"
     CDS             5290..6108
                     /locus_tag="PWX02_001097"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
                     /protein_id="HDL1729121.1"
                     /translation="MNYQAIAFDLDGTLLSSNATILESSKEAIKKVREKGIKVYFVTG
                     RHHTAVRPYYVELGLDTPVVCCNGTYVYDFKNEKVLSGNPLSAEIASKLVNDAQAQGI
                     HTAVYFEDAMTYESLNIHFTKFQKWVESCAPEVRPNVYQVENFQQEIDKGVTVWKVLI
                     SDPDLAKMQKFVEKLPPDQVSPEWSWVDRVDITSVGNSKGARLAEVLKTEGIDLQKVI
                     AFGDNFNDVSMLETVGLGIAMGNSEPDIQQKAKKTIGSNNEDSIANELKAQFNL"
     gene            6111..7802
                     /gene="narQ"
                     /locus_tag="PWX02_001098"
     CDS             6111..7802
                     /gene="narQ"
                     /locus_tag="PWX02_001098"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HDL1729122.1"
                     /translation="MIKAKYSIARRIGLYFLIMIGFASIISGISLGIMWSNKSDASLI
                     NVSGSLRMQSYRLLSEMDSKKALLPERLLEYHRTLHATELSSLKQSVLLSDSVIESYQ
                     QLLKDWYEMQSYVAQRDKVNYASHIEDYVQRVDDFVFRLQEFAELKLKIATGVIAIAM
                     LLIIALAYIGVWYTRKKIIGPLRQLVKASWQIQNKDFDHVKLAVNEPNELGLLSSTFT
                     EMSNELLKLYTSLEDKAQDKTRRLLAVNRSLLVLYQCSQLLTAKPINKEVLNQVLKTV
                     FDNEHLLGIDLQVYGAEYWNVSIDNKPSLDWVSAEIAIENEKLGILSWKPSLVCPDGR
                     LIQNVSEMIGRSLYVVQMQKQQQQLVLMEERAIIARELHDSLAQSLTFFKIQVSLLKL
                     AKTDEKRNEILTVFEKALNDAYSQLRELLTTFRLTIQEANLQQALEKILESLRPKTSA
                     QIRLSCKLPSHMFNAQQQVHVLQIVREAVINAIKHSQATEINVIAETNDDGEHCLTIQ
                     DNGVGLAAHTQPEGHYGLTIMQERAAELKAEFSIENVESGGVKVQVLLPSMISKR"
     gene            7853..8371
                     /locus_tag="PWX02_001099"
     CDS             7853..8371
                     /locus_tag="PWX02_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL1729123.1"
                     /translation="MQFRKAVEADFETILAIYNQAIPTHQITADLELATTQNRRKWFD
                     FHLETAQYPIWVVEDEQGIAGWFSFSPFYERPAFLHTSEISIYLDRYSKGKGYGSKII
                     EFMQAEMLNHNIHTLMAYVFELNTVSQNLMRKHGFEQWGRFPNIANMGKDEKGEDKWR
                     TLLMMSYQKGIE"
     gene            8414..9013
                     /gene="rdgB"
                     /locus_tag="PWX02_001100"
     CDS             8414..9013
                     /gene="rdgB"
                     /locus_tag="PWX02_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HDL1729124.1"
                     /translation="MEKTKIVLATSNADKVKEMADVLSQFGFEVVAQSEFGIESPEET
                     GLTFVENALIKARFAAKMTSLSAIADDSGLSVMALGGEPGLYSARYAGEQATDADNRQ
                     KLLAKMQGQENRLAKFVSCIVFLKHETDPTPYIASGECFGEILTEERGENGFGYDSLF
                     FYPPKACTFAELETKEKKAISHRAIALEQLKQQLAGENK"
     gene            9010..9330
                     /locus_tag="PWX02_001101"
     CDS             9010..9330
                     /locus_tag="PWX02_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HDL1729125.1"
                     /translation="MIITTTPTIEGKQIVEYKGLVFGEVVSGANFIRDFLAGITDIIG
                     GRSGAYESKLNGARKEALAELAKEAQKVGANAVVGVSMEYQSMGGDKGMFIVVAIGTA
                     VVIR"
     gene            9334..9786
                     /locus_tag="PWX02_001102"
     CDS             9334..9786
                     /locus_tag="PWX02_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HDL1729126.1"
                     /translation="MQFEQHFEANFWQNKSLLEMNDAEWEALCDGCGKCCYRKYIQGR
                     GRRERLYYTSVACNLLDVNTGRCMNYSNRFKIESDCTKLTKKNLPDFGWLPKTCAYRL
                     LYEGKPLFDWHPLISKDPDSVKNAGMLIANGIHEKDVIDWFEFVIDEV"
     gene            complement(9827..10408)
                     /gene="lpcA"
                     /locus_tag="PWX02_001103"
     CDS             complement(9827..10408)
                     /gene="lpcA"
                     /locus_tag="PWX02_001103"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HDL1729127.1"
                     /translation="MYLEQIKAELQEAADVLDKFMSDEKNIKLIQDAAMLIANSFKQG
                     GKVISCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHLSCVSNDFGYEYVFS
                     RYLEAVGQKGDVLFGLSTSGNSKNVLNAIKVAKEKGMKVIAMTGKDGGQMAGLADVEI
                     RVPHFRYADRIQEIHIKVIHILMMLIEFEMEKA"
     gene            10717..11676
                     /locus_tag="PWX02_001104"
     CDS             10717..11676
                     /locus_tag="PWX02_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL1729128.1"
                     /translation="MVVNPESFEQIIGQSVKIKEVVEQAKKFANLDAPLLIQGETGTG
                     KDLFAKSCHHFGSRRMQKFIAVNCAGLPAEEAESEMFGHRGNGVESVGFFEYAHGGTV
                     LLDSISELSLEMQAKLLRFLNDGSFRRVGEDQEIQVNVRVICTSLQPLSLLVEKGKVR
                     EDLFHRLNVLTLNLPPLRDRKEDIPLLADYFIAQISNQLGVSKLEYDNVFLQALQNYH
                     WRGNLRELYNALYRACTLSHSYRLAVKDLHLPRDMEAMIEVREEATLEEMMSQYEAQL
                     LHQFYAEYPSTRKLAQRLGLSHTAVANKLRQYGLTKNGNSKDE"
     gene            11669..13420
                     /locus_tag="PWX02_001105"
     CDS             11669..13420
                     /locus_tag="PWX02_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1729129.1"
                     /translation="MNKLAVIFSVCFANLGLNSTAYAAPSIPQELLENSLVYCTTSSG
                     FSFNPQKSDVSSNMNVVTEQIYDKLVEFNPKKNRLEPALAESYSISDDGLVITLRLRK
                     NVAFHTTPWFTPSRKLNAEDVVFSLKRMIGKESDLPEVKSEPESVLHHQRQSYIYQKT
                     AGQIYFPYFESVDLANKIASIHSPSNDIVKIELVRPDSALLAHLASQYAVIMSKEYAL
                     QLNADDNLVQLDLLPVGTGVYQLESYENNDYVRLKPNMNYWGKKAYIQNMVVDFSTEA
                     TGRMAKFLNNECDVVAFPEPSQLSVLNNRQLISSKGANLVYLAFNMQRGIGQDLALRQ
                     RIARGINRKRIAEKLFYGTGEVADNLLPTALWSKKNNGSYPYQPHQNSGEQAVEMHDS
                     IINRSLDSKTETEEVNQKQRLKLWVIDEKRVYNQHPLKMAEMIRADLATQNLEVEIRA
                     VSRAYLVQQLANKSADYDLILGGWLANNLDPNSFLEALLSCKAAETVTNLSNWCNEDF
                     DFWLQTARLSDDPASANLLYEFAQKLLEEQLPILPLMNANRVLVIKDGVKQADISPFG
                     QVKLSELRLGLGHKKGN"
     gene            13423..>13729
                     /locus_tag="PWX02_001106"
     CDS             13423..>13729
                     /locus_tag="PWX02_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HDL1729130.1"
                     /translation="MLIVFFRKIFLTLITLIFLSLISFNILLRDPLNHLAELGWFEAY
                     CYYVKSLLNGDFGISFSNGEPLTAQILQVFLATITLCLSALIVSLIIGLPLGFFAATQ
                     "
BASE COUNT         4491 a         2201 c         2929 g         4108 t
ORIGIN      
        1 atagttcaaa tgtagtggta tttagattcg gtttaagatg atggattttc attgatataa
       61 aaaaattagg ggtattgagt taaaattagc tgatttttcg tgttgtgaca atagattaaa
      121 tgaatttttt cttggtaaaa tagtctactc agaaaaatat gtaaatttta gtaaatttta
      181 aaaatagcgg gatatatttt gcaacatgta attttagcga gagatcgtaa gcgacataaa
      241 aaacatttta agattgcggt tttcttatta gcggtattta gtctatttgt tggtattgtt
      301 ctcgcactaa aagatcaatc ctcaatcaat aatacatctt cacaggaaga tattattgcc
      361 attgataaac aggaaaaata tcagagtatc aacactaatt tggcggaaaa taatactctc
      421 catcaggagc aacattccag ttttattaat tatgtgactg atttactgac tcagtcggca
      481 ttagtaatcg aacatgacga cgaaattgtt gatgatcaaa cagcgacttc ttatgaagat
      541 gaattagttg ggcaagacga tgaagtagaa gaagggattg aaacggttga aaatcaagtt
      601 cctggggaaa aactcccaga agaagctgaa aaggcattag aagatttact tgatgtggct
      661 gatcaagcac tacgaattca aaaccaattc agttataccg tagctcgagg cgataaactt
      721 aaagatgtat tagagcaatc aggcttaact gcatccgttg cgaaaaattt aattaagcaa
      781 cattcggcat tagcacaatt agaaccgggt caacaatttt attgggtatt agataatgac
      841 ggtgaattag aatatatgaa ctggctggtt tccgaaaaag aggaacgggt ttatgagcga
      901 aaagaaaatg gtaaattttc ttttcaaaga tttgagaaaa aaggggaatg gaagttagat
      961 gttgtaagag gaactattca gaatagtttt gcggttagct taaaagccgt tggcttatca
     1021 gaccgccaaa ttaaccagct agcggttgga ttgcagtcac aaattgcgac gaataaatta
     1081 aaaaaagggg ataggtttgc aattctgatt aagcgggaat atattgacgg caaagtaacg
     1141 gatttaggaa atgtggaagg aatccatgta attagcagta aaaaaagtta ttatgctatt
     1201 caagcagata atggacgcta ttatagtcga tatggtgaaa cattaagtgg aggatttgct
     1261 cgtcatccat tattatttgc agcaagagta acttcttctt tcaaccccaa acgcagacac
     1321 cctattacag ggagaattag tccgcataaa ggggttgact atggcgtgcc aataggtact
     1381 ccgatcatcg caccaagtga tggtgtcgta gagcatgtgg catatcaagc ccgtggagca
     1441 ggacgttata ttcggataaa acacggacat attacgactg tttatatgca cctcagcaaa
     1501 acgttggtaa aaccagggca gagtgttaaa aaaggggagc ggatagcatt atcgggtaat
     1561 acaggcggtt caaccgggcc tcatttacac tatgaattcc atattaatgg tcgaccggtc
     1621 aacccgatta cggtaaaatt gcctggctca aattcaggta tgggtaataa agaacgtcaa
     1681 gcattcttaa ataaagcgaa gaatgtagaa agtagattaa aaatgtaagg attctcaatg
     1741 aaaaaaaatc taatcacttt aggattaatg tgtttatcaa ccatcacgtt tgcttcagat
     1801 gcaaaactaa aaagccagct tgaaaaaatt ggagccacag atgttaaaat ttcagattct
     1861 ccattaacag gctttaaaat ggcgacttca aatgaaggtg ttgtatatat cagccaagac
     1921 ggtcgttatg taattcaagg taaagtattt gagctaaaaa atggtaaagc gattgatatt
     1981 actaatcaag gattgttggc agaattgaat tcatacacta atgaaatgat cgtatatcca
     2041 gcgaaaaatg agaaacatgt gattacggtg tttatggata ttacttgcca ttattgccat
     2101 ttactacata ctaaaatcaa agaatataat gatttaggta ttaccattcg ctatttggca
     2161 ttcccgcgtg gtggaatgaa tacccaaaca gcaaaacaaa tggaagctat ttggacatca
     2221 gcagacaaag taaaagcact gaatgatgct gaagatggta aattaccaac ccaaataaaa
     2281 acgccggata ttgtgaaaaa acattaccaa ttagggatta aatttggtgt gacaggtacc
     2341 ccaaatatgg taacttcaga aggtgagttg attggtggtt atgtagagcc taaagaatta
     2401 gcgaaaatgt taagtgagta attcattaac ttgaatacaa gcggtctttt tttgtaaaat
     2461 ttttgcaaaa gaagaccgct tgttgtttca ttaaagaaga gtattgtgga aaaaataatt
     2521 aaaaggcgcc cattattaac aacggaaata gtctctacaa acccgttatt aaatcgaatc
     2581 tatcaaagtc gaggcataaa gaatacgcaa gaactagagc ataatttaaa gaatttgcat
     2641 agaccacagc aacttgccaa tattgagcaa gccgttgact tgctattaca agcctttcgt
     2701 gaaaaaagcc ggatcattat tgtgggtgat tttgatgcag atggtgcaac cagtacatcg
     2761 cttgccctta ttgcgttacg ccaattgggt ttcgataagg tagattattt aattccggat
     2821 cgtttttctc aggggtacgg attgagtgta gcggttgctg aaatggtatt agcaaaaggg
     2881 gcggatttag tcttaacggt agataatgga atttcttcga ctgaaggcat tgctctgcta
     2941 aaaagccatc agattcaagt tttggttacc gatcatcatt taccgccaga gagcctaccc
     3001 aatgcagatg cactggtaaa ccccaattta gctttttgtg aatttccgtc taaatcttta
     3061 gccggggtag gcgtgatctt ctatgtaatg atggcattgc gttctcgtat gagagaacaa
     3121 catttatttg aaaataaacc agagccaaat tttgctgaat tactggattt agtcgcttta
     3181 ggcacagtgg ctgatgtagt gccgttagat caaaataatc ggatattggt gcatcaaggg
     3241 ttacagcgaa tccgttcagg gcattgccgt ttagggataa aagctttagc ggaggtgggc
     3301 aagcgagatc tttctacttt gcaagcagca gatttgggtt ttgctatcgc accacgtttg
     3361 aatgcagctg gtcggttaga aaatatgtct ttaggggttg agttactaat ttgtgaaaat
     3421 atggaattag ctcgccggct tgcgcttgat cttgattctc ttaatcaaac ccgaaaagag
     3481 tttgaacaag aaatgaaagc agaagcactg tcgatttgtg cgaatttgcc aagtcttgcg
     3541 cagtctgaac aggcacacgg aatagtgtta tatcaagcag attggcacca aggtgtaatt
     3601 gggatcttag cttctcgcat taaagatcaa tttaaccgtc ctgtcattgc ttttgcacaa
     3661 gaaagtgagg aaagtgaata tctaaaaggt tctgctcgct cgatcaacgg agtacatatg
     3721 cgagatttac tggaacatat cgatatgcga catccagact tgattgaaaa atttggtgga
     3781 cacgcaatgg cggctggttt gactatccat caatcaaaat tagatttatt taaacagata
     3841 tttgaccaat ctattaatgc aattatcgag cctgagcaat tacaaggcat catttatacc
     3901 gatggtgagt taaatgcaca agagtttact ttagccaatg ccgagatgat taggcaaggt
     3961 gggccttggg gacaacattt tccagaacct agttttgaag gggagtttag cattctccaa
     4021 caaaaattac tcgcaggtaa gcatttaaaa ttaatgttgc aatcagagaa agggcaacta
     4081 tttgatgcaa tttggtttaa tgtggatgta agggcgtttc ctgacttatc gatcaaaaag
     4141 gcaagattag tttataggtt ggatattaat gaatttcgag gagagaaatc tttgcaatta
     4201 aaagtggagt atcttaaata tatttgaaat gttattttac gtaaatttaa tagaattaat
     4261 tatatttgta ttgtagaatc cgtccgaatg cgactttaat aggttgtgaa gctatttttt
     4321 tatatagatt actataagga aacttaatat gaaaatggaa aaaaagccct tttccgtgga
     4381 tttttattat cgacagtggc attagcagtg gctgcttgtg gtaacttaag taaagtgagt
     4441 gatgaaggaa caactgaaaa cccggtattc ccgaaaatct cagaatctga atttaaccac
     4501 gatggttcac aatttggttc atggccaaac tgggaaaacg ttcgtcaaat tgaaaaaggg
     4561 atgaacaaag atcaattata taatctaatt ggtcgtcttc attttgaaga aggcttatat
     4621 ggtgttcgtg aatgggacta tgcattcaat taccgtgaaa atggcgtaca taaaatttgc
     4681 caatttaaaa ttttattcga taaaaatatg aatgcacaaa gtttcttctg gtatccaaat
     4741 ggatgtaatg gaaactcatc atttacttta actggcgatt tcttattcga tttcgataaa
     4801 gcgactttaa ctacaaaagg taaagaagtt gttgataatg ttgctcaaca attaaaagca
     4861 tctaaagcac aacaagttaa agtagcaggc tatacagacc gtttaggctc tgctgcttat
     4921 aacttagact tatcacagcg tcgttctaat acagttaaag ctcgtttagt acaacaaggc
     4981 gttacagcac aaattgaagc ggtaggttat ggtaaagcga atcaagtaaa agcttgtgat
     5041 ggtgaaactg gtcaagaatt aaaagattgt ttacgcccta accgtcgtgt tgaaatttct
     5101 gcaaatggtg gtgtaatgaa acaaaatgaa ggtggcaatg ttgcaggtcc taatggtccg
     5161 gctccacttt atcaaacacc agcatatgat ggcagtaaat aatttctagt tttgactaac
     5221 ttagagtaga atattaaggc aaccaaatgg ttgccttttc ttttatctat ttatttatag
     5281 gggaatacta tgaactacca agctattgca tttgatttag acggaacact actttcatcg
     5341 aacgcaacaa ttttagaatc cagtaaagag gctattaaaa aggtaagaga aaagggaatt
     5401 aaagtctact ttgttaccgg taggcaccat actgcggttc gcccttatta tgtagaacta
     5461 gggctggata cgccagttgt gtgttgcaat ggcacttatg tgtatgattt taaaaatgaa
     5521 aaagtattaa gcggtaatcc gttatcagca gaaattgctt caaaattagt taatgatgca
     5581 caagctcaag ggattcatac tgcggtttat tttgaagatg caatgactta tgagtctttg
     5641 aatattcact tcactaaatt ccaaaaatgg gttgagtctt gcgcaccaga agttcgtccg
     5701 aatgtttatc aggtagaaaa tttccaacaa gaaattgata aaggtgtaac cgtatggaaa
     5761 gtgcttatta gcgatccaga tcttgccaaa atgcaaaaat ttgttgaaaa attaccgcct
     5821 gatcaagtaa gcccggaatg gtcttgggtt gatagggttg atattaccag tgttggtaac
     5881 agcaaagggg caagattagc ggaagtatta aaaacagaag gtattgattt acaaaaggtg
     5941 attgcctttg gcgataattt caatgatgtt tctatgctag aaaccgttgg attaggcatt
     6001 gcaatgggaa attcagaacc agatattcag caaaaagcga agaaaactat aggttcaaat
     6061 aatgaagata gtattgctaa tgaattgaaa gctcaattta atctttaatt atgataaaag
     6121 caaaatactc tattgctagg cggataggac tctatttttt aattatgatc ggatttgctt
     6181 cgattatcag tggaatctca cttggtatta tgtggagcaa taaatctgat gcgagcctga
     6241 ttaatgtttc aggctcactt cgtatgcaaa gttatcgatt gttatcagaa atggattcaa
     6301 aaaaagcgct gctacctgag cgattgttgg aatatcatcg aacgctacat gctacagagc
     6361 ttagttcatt aaagcaaagt gttttattgt cagatagcgt gatagaaagc tatcaacaat
     6421 tacttaaaga ttggtatgaa atgcaatctt atgtcgctca gagagataaa gttaattatg
     6481 cttcgcacat tgaagattat gtgcagagag ttgatgattt tgttttccgc ttacaagagt
     6541 tcgctgagtt aaaattaaag attgcaacag gcgtgattgc gattgcaatg ttactgatta
     6601 ttgcccttgc ttatattggc gtttggtata ctcgaaaaaa gattattgga cctttaaggc
     6661 aattggtaaa agcgagttgg caaattcaaa ataaagattt tgaccacgta aaattagcgg
     6721 ttaatgaacc caatgaatta gggctacttt cctctacttt tactgagatg tctaatgaat
     6781 tattaaaatt atatacttca ttagaagata aagctcagga taaaacacgt cgtttattgg
     6841 cggtaaatcg ttctttactg gtattatatc aatgctctca attattaact gctaagccga
     6901 ttaataaaga agtgttaaat caagtactta aaacggtatt tgataatgaa catttgctag
     6961 ggattgattt acaagtatat ggggctgaat attggaatgt atcaatagac aataaaccaa
     7021 gtttagattg ggtttctgct gaaattgcaa tagaaaatga aaaattgggt atcttaagct
     7081 ggaaaccctc actggtttgt cctgatggac gattgatcca aaatgtgtcg gaaatgattg
     7141 gtcgtagcct ttatgtagtg cagatgcaaa agcaacagca acaattggta ctcatggagg
     7201 agcgtgcaat tattgctcgt gagttacacg attccttagc ccagtctctt acgtttttca
     7261 aaattcaggt tagcctgctt aagcttgcta aaacagatga aaagcgtaat gaaattttaa
     7321 ctgtatttga aaaagcgtta aatgatgctt acagccaatt aagggagctg ttgacgacgt
     7381 ttaggctaac gattcaagaa gcgaatttac aacaagcgtt agaaaaaatt ttagaaagct
     7441 taagaccgaa aacctcagca cagattcgct taagttgcaa attaccttcg catatgttta
     7501 atgctcagca gcaagttcac gtattgcaaa ttgtacgaga agctgtgatt aatgctatta
     7561 agcattcgca agccactgaa attaatgtga ttgctgaaac aaatgatgat ggtgaacatt
     7621 gtttgactat tcaagataat ggcgtagggc ttgctgccca tacgcaacca gaagggcatt
     7681 atggactgac gattatgcaa gaacgggcgg ctgaactcaa agccgaattt tcgattgaaa
     7741 atgtggaaag tggcggagta aaagtacagg ttttattgcc gagtatgatt agcaagcggt
     7801 aataaaatat taattttttg cgtattattg gcaacgaata aaaggataac aaatgcaatt
     7861 ccgaaaagca gtagaagcgg attttgagac aatattggca atttataatc aagcaattcc
     7921 cactcaccaa attaccgccg atttagaact agcaactacg caaaatcggc ggaagtggtt
     7981 cgattttcac cttgaaacag cccaatatcc tatttgggtg gtcgaagatg aacaaggcat
     8041 tgctggctgg tttagttttt caccatttta tgaaagaccg gcatttttac atacttcgga
     8101 aatcagtatc tatttagacc gctattccaa aggaaaaggc tatggctcaa aaattattga
     8161 gtttatgcaa gctgaaatgc ttaatcacaa tattcatacg ttgatggcgt atgtgtttga
     8221 attaaataca gtgagccaaa atctaatgag aaaacacggc tttgaacaat ggggaagatt
     8281 tccgaatatt gctaatatgg gaaaagatga gaaaggtgag gataaatggc gaacgttgct
     8341 gatgatgtct taccaaaaag gtatcgaata acaagcggtg attttttaga atatttttgc
     8401 aaataaggac aatatggaaa agacaaaaat tgtacttgca acaagtaatg cagataaagt
     8461 taaagaaatg gcagatgtac tctcacaatt tggttttgag gtggtggctc aaagtgaatt
     8521 tgggattgaa tcaccggaag aaaccggttt gacttttgtt gaaaatgcgc tgattaaagc
     8581 acgatttgct gctaaaatga caagtttatc ggcgattgcc gatgattcag gtttatcggt
     8641 tatggcactt ggtggtgaac cggggctata ttctgcacgt tatgccggag agcaggcaac
     8701 cgatgccgat aatcgccaaa aattactagc caaaatgcaa gggcaagaga atcgtctggc
     8761 gaaatttgtc agttgtatcg tgtttttaaa acacgaaact gacccaacac cttatattgc
     8821 gtcgggtgaa tgtttcggtg aaattttgac ggaagaaaga ggtgaaaatg gtttcggcta
     8881 cgattccctg tttttctacc caccgaaagc ttgcactttt gccgaattag aaaccaaaga
     8941 gaaaaaagcg atttcacacc gtgcaatcgc actagagcaa ttaaaacagc aattagcagg
     9001 agagaataaa tgattattac aactacacct acgatagagg gtaaacaaat tgtagaatac
     9061 aaagggttag tatttggcga agtagtttct ggggctaatt ttatccgtga tttcttagcc
     9121 ggtatcaccg atattattgg cggacgttca ggtgcttatg aaagcaaatt aaacggtgca
     9181 agaaaagagg cattagctga attagctaag gaggcacaaa aagtgggggc aaatgcggtg
     9241 gttggcgttt ctatggagta tcaatcaatg ggcggcgata aaggaatgtt catcgtggta
     9301 gcgattggta ctgcggttgt aatacgttaa tttatgcagt ttgaacaaca ctttgaagca
     9361 aatttctggc agaacaaatc gctacttgaa atgaatgatg ctgaatggga agcattatgt
     9421 gacggttgtg gtaagtgctg ctaccggaaa tatattcagg gaagagggcg gcgtgaacgg
     9481 ctttactata cctcggttgc ctgtaattta ttagatgtga atacaggccg ttgtatgaat
     9541 tactcgaacc gttttaaaat tgagtcggat tgtacaaaat taactaaaaa gaacttacct
     9601 gattttggct ggttgccaaa aacctgtgct tatcgtttac tttatgaagg aaagcctctt
     9661 tttgattggc accctttaat ctctaaagat cctgattctg taaaaaatgc cggaatgctg
     9721 attgctaacg gtattcacga aaaagatgtg attgattggt ttgagtttgt tattgatgaa
     9781 gtatgagaaa aaggcgtttg gtgcaaacgc ctttttgtta tagagattag gctttttcca
     9841 tctcaaattc aattaacatc atcaaaatat gaatcacttt aatgtgaatt tcttggatgc
     9901 ggtctgcgta acggaaatga ggtactcgga tttccacatc ggctaatcct gccatttgcc
     9961 cgccatcttt gccggtcatt gcaatcactt tcatgccttt ttctttggca actttgatcg
    10021 catttaacac atttttagag ttaccagagg ttgatagacc aaagagtaca tcgccttttt
    10081 gcccaacggc ctctagataa cgagagaaga catattcata accgaagtca ttacttacac
    10141 agcttaaatg gctgacatcg gaaatagcga ttgccgggta tccgggacgg ttttcacggt
    10201 aacgacctgt taattcttcg gcaaagtgca tcgcatcaca gtgcgaaccg ccattaccgc
    10261 aagaaatcac cttaccacct tgcttgaaac tatttgcaat cagcatggcg gcatcttgaa
    10321 ttaatttaat atttttttca tcagacataa atttgtctaa tacatctgca gcttcttgaa
    10381 gctcagcttt aatttgttct aaatacatca tttgctcctt tgtttgcaaa acgcttacct
    10441 ttaaacatcg gctttaccaa tgggggtagc gagtaaaaaa ttcgtttttt atgaccgctt
    10501 gaggcataaa aagtgcaata agtttagaag atctatgctg tattgtaaac ctaataactt
    10561 aaataatctg aatagggttg taaacaaaag tttccaaaag agaaaaatag cggtatgatc
    10621 gcctcacaat ccctctcgca ttgttaactc tagtagctaa tatcaggctt taagcgtatt
    10681 aaacaatgca caaatttgct atttatagga gactatatgg tcgtaaatcc agaatctttt
    10741 gaacagataa ttggacaatc tgtaaagata aaagaggttg ttgaacaagc aaaaaaattt
    10801 gccaaccttg atgcaccatt attgattcaa ggtgagacgg gaacaggaaa agatttattt
    10861 gccaaatctt gtcaccattt tggatctcgt cgaatgcaaa aatttattgc cgtgaactgt
    10921 gccggtttac cggctgaaga agcagaatcg gaaatgttcg gacatcgtgg caatggcgtt
    10981 gagtccgtcg gtttctttga atatgctcac ggtggtacag tattattaga ctctatttct
    11041 gagttatcgc tggaaatgca agccaaatta ttgcgctttt taaatgatgg ctcttttcgt
    11101 cgagtgggcg aagatcaaga gattcaagtc aatgttaggg tgatctgtac ttcattacag
    11161 cctttgtcat tattagttga aaaaggcaaa gtaagagaag atctgtttca tcgtttaaat
    11221 gttctcactt taaatttacc acctttgcga gatcgaaaag aggatattcc attattagct
    11281 gattacttta tcgcccaaat tagcaatcag ttgggggtga gcaaattaga gtatgataat
    11341 gtttttttgc aagcattgca gaattatcac tggcgaggca acttaagaga gctttataac
    11401 gcactttatc gagcttgtac actttcgcat agctaccgtt tagcggtaaa agatcttcat
    11461 ttacccagag atatggaggc gatgattgaa gtacgtgagg aggcgacact tgaggaaatg
    11521 atgagccagt atgaagccca attattgcac caattttatg cagaatatcc gagtacccga
    11581 aaattagcac aacggctcgg actttcacat actgcggtgg caaacaaatt gcgtcaatat
    11641 gggttaacaa aaaatggaaa cagtaaagat gaataaacta gcggtcattt tttcagtatg
    11701 ttttgcaaat ttagggctaa attcgaccgc ttacgcagcg ccaagtattc ctcaagagct
    11761 acttgaaaat agccttgtgt attgcactac ttcatcggga ttctctttta acccacaaaa
    11821 atctgatgtg agttcgaata tgaatgtggt aacagagcag atctacgata aattagttga
    11881 gtttaatccg aaaaaaaatc gactagagcc tgctttagct gaaagttatt caatcagcga
    11941 cgatggttta gtgattacat tacgtttaag aaaaaatgtc gcttttcata caactccatg
    12001 gtttactccg agtcgtaaat taaacgcaga agatgtggtg ttttcgctaa aacgaatgat
    12061 tggtaaagaa agtgatttac ctgaagtgaa aagtgagcct gaaagtgtat tacaccacca
    12121 gcgacaaagc tatatttacc aaaaaacagc ggggcagatt tattttcctt attttgaaag
    12181 tgttgattta gcgaataaaa ttgccagcat tcattctccg tcaaatgaca ttgtaaaaat
    12241 tgaattagtc cgtcctgatt cagcattgtt ggcgcattta gccagccaat atgcagttat
    12301 tatgtctaaa gaatatgctt tacagctcaa tgcagatgat aacttagttc aactggattt
    12361 attaccggta ggaacaggcg tatatcaact tgaaagttat gaaaataatg attatgtgcg
    12421 tttaaaacca aatatgaatt actggggcaa aaaagcatat attcaaaata tggtcgtaga
    12481 tttttccaca gaagcgaccg gcaggatggc aaaattttta aataatgaat gtgatgtggt
    12541 tgcttttccg gaacctagcc aattatcagt gttaaataat cggcagctta ttagtagtaa
    12601 aggtgctaat ttggtttatt tagcattcaa tatgcaacgg ggtattgggc aggatttggc
    12661 attacgccaa cgtattgcac gcggcataaa tcgcaaacgt attgcagaga aattatttta
    12721 tggtacaggt gaagtagcgg ataacctatt accaacagct ttgtggtcga aaaaaaataa
    12781 tggaagctac ccttatcaac cacaccaaaa ttcaggtgag caagcggttg aaatgcatga
    12841 cagtattata aatcggtctt tagatagcaa aacagagacc gaagaagtaa accaaaaaca
    12901 gcgcttaaaa ctgtgggtaa ttgatgaaaa acgagtttat aatcaacacc cattaaaaat
    12961 ggcagaaatg atacgtgctg atttagctac gcaaaattta gaggtcgaaa ttcgcgcagt
    13021 aagtcgagct tatttggtgc aacagcttgc taataaaagt gcggattatg atttgatttt
    13081 aggtggttgg ttagcaaata atcttgatcc taacagtttt ttggaggcct tactcagttg
    13141 taaagctgcg gaaacggtga ctaatttatc aaattggtgc aatgaagatt ttgatttttg
    13201 gttacaaaca gcccgtctaa gtgatgatcc ggcatcagct aatttattgt atgaatttgc
    13261 acaaaaatta ttagaagagc aattgcctat tctcccttta atgaatgcta atcgtgtatt
    13321 agtcataaaa gatggagtga aacaggcaga tatttcccct ttcggccagg taaaattatc
    13381 agagttacgt ttgggcttgg ggcataaaaa aggaaactaa ttatgctgat tgtctttttt
    13441 cgaaaaattt ttctaacctt gattacgttg atttttttgt cacttatcag ttttaatatt
    13501 ctgttacgag atccattaaa ccacttagcc gagttaggct ggtttgaggc ttattgttat
    13561 tatgtaaaat ctctgctaaa tggtgatttt ggtattagtt tcagtaatgg agaaccgctc
    13621 acagcgcaaa ttttacaggt gttcctggct acaattacgc tttgtttatc ggctctcatt
    13681 gtttctttaa ttattggttt gcctcttggt ttttttgcgg ctactcagc
//
LOCUS       DANJWC010000051        13631 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.051, whole genome shotgun sequence.
ACCESSION   DANJWC010000051 DANJWC010000000
VERSION     DANJWC010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13631
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.051"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(2..2566)
                     /locus_tag="PWX02_001107"
     CDS             complement(2..2566)
                     /locus_tag="PWX02_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HDL1729131.1"
                     /translation="MRIAKIIFSALLSLLVLGGVIGGLLYMHFKSDLPDVATLKNVEL
                     QQPMQIFTLDGKLIGEVGEERRIPVKLDDIPPMLVKAILATEDARFYEHKGIDPKGIM
                     RAIWRTSQGDTQGASTITQQLAKNFFLTPERSLERKAKEAILALEIEQVLSKNEILEL
                     YLNKIYLGYRSHGVAAAAKTYFNKALKDLTLSEIAIIAGLPKAPSTMNPLYSVKRAED
                     RRNVVLGRMLETGDITKEEYEKAKAEPVVANYYGATLEFRADYVTEMVRQEIVKRYGE
                     NIAYNKGLKVYATVLSADQKAAQDALRENLIDYDRRHGWRGAEKLWTGSSAWDDEKII
                     DHLSKLPKSEPFTPAVVMQADKSKYTILLANGDISVLKRTNASFAPKGLKAGEQIWVR
                     QNKNREWALGQIPEVNSALVSINSENGAIESIVGGFSFEQSRFNRATQSLVQVGSAIK
                     PFIYTAAMNKGLSLSTTISDSPIVIKKKGQKEWRPKNADGVYGGPLRTRVALGKSKNM
                     VAIRILQMAGIDYVADYLQRFGFNRNQYVATESLALGAASFTPLEMARAYAVFNNGGY
                     LIEPYIIDRIIDAQGYELYQANPVVACNSCDSPVVYPEPKYFESVKIHDEAQVGVQTI
                     ANSSNNDDDDVVEDVGDSEPELQANNSEKNAPSLMAESSHHVKGLRYAPHVISDELAF
                     LMRSALATAITGEPEYGWRGTSYKMLNTIKRADVGGKTGTTNNAKATWYAGFGANIST
                     VVYVGFDDNKRVLGRGASGSSTALPAWTNYMKVALSDKPVEKSTLPKNIIEVNIDPNS
                     GFLGNGRKEYFIKGTEPTKRYIVERAYTEKAPQKTESAPLRLGLPPPGVLKSGELF"
     gene            2690..3388
                     /locus_tag="PWX02_001108"
     CDS             2690..3388
                     /locus_tag="PWX02_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComA"
                     /protein_id="HDL1729132.1"
                     /translation="MKLLKAHSKTPSNRIILGLSEDEQRYCLVKNKAEKFTVFWQAKP
                     ASIEELIGNLTPQEQAMMTLVRPIPYQYIWRKTVFMANTLNEAQLHRKVIHILKNEQP
                     LALDLLNIDYQRFPSSNDNLDKVVIYAVRKSYTENLSQFNSILDCELHCYARAIIHLT
                     QSQNNQPAPCFSFKQKYFQFTETELLILQTEPEDCIHLSDLKIDHLEIECEQEKQLYC
                     LALGASLWNGKVLI"
     gene            3364..3885
                     /locus_tag="PWX02_001109"
     CDS             3364..3885
                     /locus_tag="PWX02_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729133.1"
                     /translation="MEWQGIDLSANRKNNWFAGSDKGIKNVIYTLLLSALISLALIFL
                     YLQKNQEIQPLAQQLELVKTDILNIENKIISLNSNHTNTLPFAEPKKIAQLLAIIDNL
                     PLKNGGIETIQIYLETDLYLKIIGNLDNQSDFQSLESYLYQQDLFETKTEQINVNHKN
                     EIQFIFTLKHKGN"
     gene            3887..4423
                     /locus_tag="PWX02_001110"
     CDS             3887..4423
                     /locus_tag="PWX02_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729134.1"
                     /translation="MFSIHSKIEAAYSNPNSSLFQFICFIRKYPKALPFGIMMIFIIP
                     PMIMLIKSYIEVNHILKNIENDKINFNHQQNKYDSIINKAKNQNSQENNLTEINSNIQ
                     ILAEKHQLTIESLQWNLEQGKSIELSIIGNSRSLFDFIHSVKQIPYLKYNAISLIKSR
                     QDRKVEMNTTLVILANKE"
     gene            4426..4839
                     /locus_tag="PWX02_001111"
     CDS             4426..4839
                     /locus_tag="PWX02_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729135.1"
                     /translation="MRNWFLFITLFISFSVKANDPFFGEKQETQASGNIAESNVEFAK
                     NTENITACKVPDNLSAKNIVTDFDKMTLIGLVKINEVMRALFIDEKQQLLALKENDLL
                     NNEIEIKHINLKSITYINWKLTKDCNIPYEINIKL"
     gene            4848..6161
                     /gene="pilQ"
                     /locus_tag="PWX02_001112"
     CDS             4848..6161
                     /gene="pilQ"
                     /locus_tag="PWX02_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HDL1729136.1"
                     /translation="MWRIFIYFLCGFSLAYANTISIVLKNAPTSLIFTYLAEETGKNM
                     VLDDNIETKSTLRLENKSVDEIFKTLGKVNKLSLTQEDDIVYIHKKEEKAADLTPLPI
                     MNLQNNGQNTPLVTAPKLITKTLKLHYAKASEVIESLTKGSGTFLSENGYIHFDERSN
                     SLIVKDSAKSLKNIENLVKQLDQPTEQIAIEARIVTISSEHLQELGVRWGIFSRGAGH
                     YKFGGRLEGNGLNDVTNNLNVNFPITGGASAVLQVASINSRVLDLELSALEQENSVEI
                     IASPRLLTTNKKPASIKQGTEIPYVMYNTKSEATDVEFKEAVLGLEVVPHLSTQNQIL
                     LDLIVTQNSPNSQSGSSGLITIDKQELNTQVFAKHGETIVLGGIFQHLTQKGEDKVPI
                     LGSIPFIKRLFSQTRDKISKRELVIFVTPYILQSNEKSSNHKKSK"
     gene            6242..6655
                     /gene="nusB"
                     /locus_tag="PWX02_001113"
     CDS             6242..6655
                     /gene="nusB"
                     /locus_tag="PWX02_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDL1729137.1"
                     /translation="MKISPRRRARECAVQAIYSWYISQNTVEEVELAFVTDQDMKGVD
                     MPYFRKLFRGVVDNVEAIDEALRPYLDRKESDVDPIERSILRLSTYELKFEHDVPFRV
                     VINEGIEVAKVFGSDDSHKYINGILDKLAPALGRK"
     gene            6718..7698
                     /gene="thiL"
                     /locus_tag="PWX02_001114"
     CDS             6718..7698
                     /gene="thiL"
                     /locus_tag="PWX02_001114"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HDL1729138.1"
                     /translation="MGEFDIIERYFNASQRPPRKDVLLSIGDDCALTSLKPNQQLAIT
                     TDTLVCGTHFLPTISPADLAYKSVASNLSDLAAMGAEPAWISLALTLPEINHDWLAEF
                     SEHFFEILDHYNVDLIGGDTTCGPLSLTITAQGIVPQDRGLFRHNAKVGDWIYVSGTL
                     GDSAGGLQLLLNQQSEQVNWNKEQSYLIQRHLRPTPRVLLGLTIASIANAAIDISDGL
                     LSDLSHILKRSDCGAVLNLENFPLSDVLLSCYSRTEAEYFALNGGEDYELCFTVPNDN
                     KAKLDKALANIGVTYTCIGQIRSAKEGLTLLRHNQPVTLPSQRGYDHFKK"
     gene            7703..8176
                     /locus_tag="PWX02_001115"
     CDS             7703..8176
                     /locus_tag="PWX02_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDL1729139.1"
                     /translation="MKPINLKNPIHFFAFGFGSGLLKPAPGTWGSLAGLIVAIALWSI
                     TQSALFFGLLTLVSFIAGCVICTKASQDLEVHDDGRIVWDEIVAIFLMFTCLPEYNLL
                     GYLLAFISFRIFDILKPFPIRYFDEKLESGLGIMFDDILAAIFALISLHLLYYFI"
     gene            8183..8806
                     /locus_tag="PWX02_001116"
     CDS             8183..8806
                     /locus_tag="PWX02_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HDL1729140.1"
                     /translation="MLTILFIHFAGLISPGPDFFYVVRQSASHSVKSGVLAATGISIG
                     IIFWAAFAIFGLAWLSNSIGSLFQYAIMIIGGCYLVYIGSQMVRVTENIIFVENKAQL
                     TPLNPWQEIKKGLLINIFNAKAGVYFTSVISAYVDNLSNTLQMFELLLLFVVSTFIYF
                     CVVALLFSRRPVRLFYAKYSRYIDNISGVIFILFGSMLIYEGFSHFI"
     gene            8918..9523
                     /gene="ruvA"
                     /locus_tag="PWX02_001117"
     CDS             8918..9523
                     /gene="ruvA"
                     /locus_tag="PWX02_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005686751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDL1729141.1"
                     /translation="MIGRLHGKIIEKQPPEILLDVQGVGYELLLPMTSFYSLPQIGEE
                     ATIFTHLVVREDAHLLFGFAQKQDRTLFRELIKTNGVGPKLALAILSAMSVSQFATAI
                     ENEELAKLTKIPGIGRKTAERLLVELKGKFKRMAQSDFFVEVSHEQIVASNTPDPANE
                     ALDALIALGYKPADAEKMIKKVNKSGATSEQLIREALKNSL"
     gene            9660..10664
                     /gene="ruvB"
                     /locus_tag="PWX02_001118"
     CDS             9660..10664
                     /gene="ruvB"
                     /locus_tag="PWX02_001118"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDL1729142.1"
                     /translation="MIEADRIISASPKREEEVIDRAIRPKLLADYVGQPSVRDQMEIF
                     IKAAKLREEALDHLLIFGPPGLGKTTLANIVANEMGVNIRTTSGPVLEKAGDLAAMLT
                     NLEPYDVLFIDEIHRLSPAIEEVLYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLVGA
                     TTRAGSLTSPLRDRFGIVQRLEFYSVDDLTLIVKRSADCLNLNLSADGAYEVARRSRG
                     TPRIANRLLRRVRDYADVRNNGIITSDIAKQALAMLDVDSEGFDFMDIKLLQAIVERF
                     DGGPVGLDNLAAAIGEERDTIEDVLEPYLIQQGFLQRTPRGRIATSRTYAHLGITQID
                     "
     gene            complement(10763..11446)
                     /gene="artM"
                     /locus_tag="PWX02_001119"
     CDS             complement(10763..11446)
                     /gene="artM"
                     /locus_tag="PWX02_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HDL1729143.1"
                     /translation="MWWDYLVEISQGIGTSLQLTFVALIIAFILAIILTFILSLENRP
                     LKWLVNGFITLFTGTPLLIQFFLIYSGPGQFEWITESAAWVLLSDAWFCAMLALALNS
                     AAYSTLLFHGAVKAIPKGQWETCAALGLSRLDTLKILVPYALKRALPSYSNEIVLVFK
                     GTSLASTITIMDIMGYARQLYGTEYDALSIYGMAGVIYLVITGIMTIVLRKLESRVLA
                     FERFETEKA"
     gene            complement(11446..12117)
                     /gene="artQ"
                     /locus_tag="PWX02_001120"
     CDS             complement(11446..12117)
                     /gene="artQ"
                     /locus_tag="PWX02_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HDL1729144.1"
                     /translation="MSSDILNLLVNATLMTLGIALASLLIGMLLSIFFVVLETSKIAC
                     IRKPTSIIITLLRGLPEILVVFLIYFGLIQVIFLITDEFAELNAFWCGAIALGIIFAA
                     YASQSLRGAIKAIPVGQWESGTALGLSRAQVFVRIILPQVWRHALPGLSNQWLVLLKD
                     TALVSLIGVDDLMRQSELINANTHQPFTWYGIAALIYLAITLISQRVIKVLEGRFTRF
                     ERGVA"
     gene            complement(12117..12842)
                     /locus_tag="PWX02_001121"
     CDS             complement(12117..12842)
                     /locus_tag="PWX02_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL1729145.1"
                     /translation="MKKLLLASAIAFATLSANAQETITFAMEPSYAPFETTNEKGEVI
                     GFDVDIAKALCEELKATCEFKTQSFDSLIAGLKFKNFDAIISGMDITEVRQKQVAFTN
                     AYLENSVSYVAMKGKFTPETAKNVGVQNGTTFQQYLSKNAKQYNLKPYASLQSAVLDL
                     KNGRVDMVFGDTPVLAEWLKTEKELDFVGEKVVDKAFFGNGYGIAVNKSNTALLEKLN
                     SALATIKTNGKYEQIYNTWMAGK"
     gene            complement(12870..13604)
                     /gene="artP"
                     /locus_tag="PWX02_001122"
     CDS             complement(12870..13604)
                     /gene="artP"
                     /locus_tag="PWX02_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HDL1729146.1"
                     /translation="MAIRIQNVNFFYGSNQALFDINLEIEKGDTVVLLGPSGAGKSTL
                     IRTLNLMEVPQSGTLAIANHQFDLTAKTDSKAVSLLRREVGMVFQQYHLWPHLTVMQN
                     LIEAPMKVLGLSKEEAVARAKGHLARLRLSEFAERFPLHLSGGQQQRVAIARALMMQP
                     QVLLFDEPTAALDPEITAQVVDIIKELQSTGITQVIVTHEVGVARKVASKVVYMEKGT
                     IIEQGDASCFESPKTEQFAQYLSHSE"
BASE COUNT         4239 a         2863 c         2463 g         4066 t
ORIGIN      
        1 attagaataa ttcaccgctt tttagcaccc ctggtggagg taatcctaaa cgtaacggcg
       61 cagattctgt tttttgaggc gctttttcag tataagctct ctctacaata taacgttttg
      121 taggctcagt acccttaata aaatactctt tacgtccatt tcctaagaaa ccactattag
      181 ggtcaatatt aacctcaata atattttttg gaagggtact tttttcgaca ggcttatctg
      241 acaatgcaac tttcatataa tttgtccaag ctggtaaagc agtactagaa cctgaagcac
      301 ctctacctaa tactctttta ttatcatcaa agccaacgta cacaacagta gaaatattag
      361 caccaaatcc cgcataccaa gttgccttgg cattattggt tgtaccagtc ttcccgccca
      421 catccgctcg ttttatcgta ttgagcattt tatagcttgt tcctcgccag ccatattcag
      481 gctcaccagt aattgctgta gctaaagcac tacgcattaa aaaagcgagt tcatcactaa
      541 taacatgagg agcgtaacgt aaccctttga catgatgaga agattctgcc attaagctcg
      601 gcgcattttt ctcgctattg ttcgcttgta attcaggttc tgaatcgcca acatcttcca
      661 caacatcgtc gtcatcatta ttcgaggaat tcgctatagt ttgtacaccg acttgagctt
      721 catcatgaat tttgactgat tcaaaatatt taggctcagg gtaaacaact gggctatcgc
      781 aactattaca agctactaca ggatttgctt gatataactc gtagccttgg gcatcaataa
      841 tacggtcaat gatatatggt tcaatcaaat aacctccatt attaaatact gcataagctc
      901 gagccatttc taatggcgta aaagatgcag cacccaatgc aagagattct gttgcaacat
      961 actgatttcg gttaaagcca aaacgttgta agtagtctgc aacataatca atccctgcca
     1021 tttgtaatat acgaatagcc accatatttt tcgatttacc taatgcaaca cgagttctca
     1081 acgggccgcc ataaacccca tcggcatttt taggtcgcca ctctttttgc cccttctttt
     1141 taatgacaat aggagaatcg ctaattgttg ttgaaagact tagcccttta ttcattgccg
     1201 ctgtgtaaat aaacggttta atcgcagagc ctacctgaac taatgactga gtcgcacgat
     1261 tgaaacggct ttgttcaaag ctaaagcctc caacgataga ttcaatggca ccgttttcac
     1321 tattaattga cactagggct gaatttacct ccggaatttg tcctaaagcc cactccctat
     1381 ttttattttg acgtacccaa atttgttcac ctgcttttaa ccctttaggt gcaaaagaag
     1441 catttgtacg ttttaaaaca gagatatcac catttgccaa taaaattgta tattttgatt
     1501 tatctgcttg cattacaaca gctggtgtaa acggctctga ttttggcagc ttacttaaat
     1561 gatcgataat tttctcatcg tcccaagcag aactacctgt ccataatttt tcagcgcctc
     1621 gccaaccatg acggcggtca taatcaatca aattttcacg taacgcatct tgtgctgctt
     1681 tttggtctgc tgaaagtacc gtggcgtaca cttttaagcc tttgttataa gcaatatttt
     1741 ctccatagcg tttcacaatt tcttgacgaa ccatttctgt cacataatcc gctcgaaatt
     1801 ctaatgtcgc accatagtag ttagctacta ctggctctgc tttagctttc tcatattcct
     1861 cttttgtaat atctccggtt tctagcattc tcccaagcac tacattacga cggtcttccg
     1921 cccgttttac tgaataaagt ggattcatcg ttgaaggcgc ttttggaagc cctgcaataa
     1981 ttgctatttc agaaagggtt aaatctttca aagctttatt aaaataagtt ttagccgcag
     2041 cggcaacacc gtgagaacga taccctaggt aaattttgtt taaatacagc tccagaatct
     2101 catttttact gagaacctgt tcaatttcta aagctaaaat tgcctctttg gccttacgct
     2161 ctaaactacg ttcaggtgtt aagaaaaagt ttttcgccag ctgctgggtg attgtacttg
     2221 cgccttgagt atctccctgg ctagtgcgcc atattgcacg cataatacct ttaggatcga
     2281 tccctttatg ctcgtaaaaa cgtgcatctt cggtagcaag aatcgctttt actaacatcg
     2341 gtggaatatc atcaagcttg accggaatac ggcgttcttc tcctacttca ccaattaatt
     2401 taccatctaa ggtaaaaatc tgcataggct gctgtaactc tacatttttc agcgttgcca
     2461 catcaggcaa atcagatttg aaatgcatgt acagtaaacc accaattacc ccacctaaaa
     2521 ccaagagtga aagtaaagca ctaaatatta tttttgcgat ccgcatcgaa aaaatctctt
     2581 ttggttaatg acgaaacata aatttaaagc caatattagg tcactaagta taagctaaaa
     2641 acgcttactt cgcataacta attgcaaaac tttacttact atacttacta tgaaattact
     2701 taaagcacat tcaaaaacac cttcaaaccg tatcatctta ggcttaagtg aagatgaaca
     2761 acgttactgc ttagtcaaaa ataaagcaga aaaattcacc gttttttggc aagccaagcc
     2821 tgcgagcatc gaagagctta tcggtaatct caccccacaa gaacaagcaa tgatgacgct
     2881 ggttcgcccg atcccttacc aatacatttg gcgaaaaacc gtttttatgg ctaacaccct
     2941 caatgaagct caattacacc gtaaggtaat tcacatttta aaaaatgagc agccacttgc
     3001 attggatcta ctcaatattg actatcagcg atttccaagc agcaacgata atctggataa
     3061 agtggttatc tacgccgtgc gaaaaagtta taccgagaat ttgagccaat ttaacagcat
     3121 cttagattgt gagctgcatt gctatgcacg ggctataatt catttaacac aatcccaaaa
     3181 taatcagcct gcgccctgtt tttcatttaa acagaagtac ttccaattta ccgagacgga
     3241 attactgata ttgcaaacag aacctgagga ttgtattcat ctatctgatc tcaaaattga
     3301 ccatcttgaa atcgagtgtg agcaagagaa acagctatat tgcctcgcct taggagcaag
     3361 tttatggaat ggcaaggtat tgatttaagt gctaatcgaa aaaataactg gtttgccggc
     3421 agtgataaag gcatcaaaaa cgtgatttat acacttttat tatctgccct cattagcttg
     3481 gctctgattt ttctctattt gcaaaaaaat caagaaattc aaccgcttgc acagcaatta
     3541 gagctggtaa aaaccgatat attaaatatt gaaaataaaa ttatcagctt gaatagtaat
     3601 catacaaaca ccttgccctt tgccgagcct aaaaaaatag cacaactact ggcaataata
     3661 gataacttac cgctcaaaaa tgggggaatt gaaacgatac aaatttatct tgaaaccgat
     3721 ctctatttaa aaataatcgg taatttagat aatcaatctg attttcaaag tctagaatct
     3781 tatttatatc agcaagattt atttgaaacc aaaaccgagc aaattaatgt taatcataaa
     3841 aatgaaatcc aatttatttt cacactgaaa cataaaggta attaatatgt tttctataca
     3901 ttctaaaatt gaagctgcat attccaatcc aaacagttca ttatttcagt ttatttgctt
     3961 tatccgaaaa tatcctaaag cattaccatt tggcattatg atgatattca tcattccacc
     4021 tatgattatg ttgataaaat cctatattga ggttaatcat attttaaaaa atatagaaaa
     4081 tgacaagatt aactttaatc atcaacaaaa caaatatgac tcaattatca ataaagctaa
     4141 aaatcagaac agccaagaga ataatttaac cgaaataaac agcaacatac aaatattagc
     4201 tgaaaaacac caactaacca ttgaaagttt acaatggaat ttagaacaag gtaaaagtat
     4261 tgaattgagt attatcggca atagccgatc tttatttgat tttatccatt cagttaagca
     4321 aataccttat ttgaaataca atgcgattag cctcatcaaa tctcggcaag acagaaaagt
     4381 tgaaatgaat actaccttag tcatattagc taataaggaa taacaatgcg taattggttt
     4441 ttatttatta ctttatttat cagtttttct gtaaaagcaa atgatccttt ctttggagaa
     4501 aaacaggaaa ctcaagcctc aggcaacatc gctgaaagca atgtggaatt tgcaaaaaat
     4561 actgaaaaca tcaccgcttg taaagtacct gataaccttt ctgcaaaaaa tatagtaact
     4621 gattttgaca aaatgacact gataggatta gttaaaatca atgaagtaat gcgtgcatta
     4681 tttattgatg aaaaacaaca attacttgct ctaaaagaaa atgacttgct taacaatgaa
     4741 attgaaataa aacatattaa tcttaaatcc attacttata ttaactggaa attaactaaa
     4801 gattgtaata tcccttatga aattaacatc aaattataga ggttgatatg tggcgaattt
     4861 ttatatattt cttatgcggt ttttcattag cctatgcgaa taccatttcc atagttttaa
     4921 aaaatgctcc aacttcgctt atttttactt atttagcgga agaaaccggt aaaaatatgg
     4981 tgcttgatga taatattgaa accaaatcaa cactacgatt agaaaataaa tcggttgacg
     5041 agatttttaa aacgcttggc aaagtcaata aattatcctt aacgcaagaa gatgatattg
     5101 tttatattca caaaaaagag gaaaaagcgg ctgacttaac gcctctgcca ataatgaatt
     5161 tgcaaaataa tgggcaaaat acaccgcttg tcaccgcccc taagttaatc acaaaaacct
     5221 tgaaactaca ttatgcaaag gcttcagagg tgattgagtc gttaaccaaa ggcagcggaa
     5281 cgtttttatc ggaaaacggt tatattcact ttgatgaaag aagcaatagc ctgattgtga
     5341 aagacagtgc aaaatcgctt aaaaatatcg agaatttagt aaaacagctc gaccaaccca
     5401 ccgagcaaat tgcgattgag gctagaattg tcactatcag tagcgagcat ttgcaagagc
     5461 ttggcgttcg ctggggaatt ttttctcgtg gggctgggca ctataaattt ggcggacggc
     5521 tggaaggcaa tggactcaac gatgtcacca ataacttaaa tgtaaatttt ccgattactg
     5581 gtggagcatc tgccgtatta caggttgcgt ccattaatag cagagtgctt gatttagaat
     5641 taagtgcatt agaacaagaa aatagtgtgg aaattattgc cagcccacgc ctgttaacca
     5701 ccaataaaaa gccggcaagt atcaagcaag gcacggaaat tccttatgtg atgtacaaca
     5761 ccaaatcgga agccaccgat gtcgaattta aagaagcggt attagggctt gaagtcgttc
     5821 cacacctttc aacccaaaat caaattctac tcgatctgat cgttacccaa aattcgccaa
     5881 attcccaatc cggcagtagt ggcttaatta cgattgataa acaagagcta aatacgcaag
     5941 tatttgccaa acacggcgaa accattgtac ttggtggtat ttttcaacat ttaacccaaa
     6001 aaggcgaaga taaagtgccg attttaggct caattccctt catcaagcgg ttatttagtc
     6061 aaactcgaga taaaatcagc aaaagagagc tagtcatttt tgtgacgcct tatatccttc
     6121 aatctaatga aaagagcagc aaccacaaaa aatcaaaata gcacatcata aacgcaagca
     6181 aaaatagggg aagtgtgata gaatcagccg tcattttttg agttaaagat ttagaggttt
     6241 tatgaaaatt tccccacgtc gccgtgcaag agaatgtgcg gtgcaagcca tttattcttg
     6301 gtatatttcc caaaacacgg ttgaagaagt ggaattggcg tttgtgaccg atcaagatat
     6361 gaaaggcgtt gatatgcctt atttccgtaa acttttccgt ggcgttgtgg ataatgtaga
     6421 agccattgat gaagcattac gcccttattt agatcgtaaa gagtcagatg ttgatcctat
     6481 tgaacgttct attttgcgtt tatcaactta cgaattaaaa tttgaacacg atgtgccttt
     6541 ccgtgtagtc atcaatgaag ggattgaagt cgctaaagtg ttcggctctg atgacagcca
     6601 caaatacatt aacggcattt tagataaatt agccccagca ttaggcagaa aataataaga
     6661 ttccccaatt tatacataaa ttggggattt tgattataac gctcaaggag attttttatg
     6721 ggtgaatttg atattatcga acgctatttt aatgcttctc aacgcccacc tcgcaaagat
     6781 gttttacttt ctatcggcga tgactgtgcg ctcacatcac tcaaaccaaa tcagcaactt
     6841 gcgatcacaa ccgataccct tgtttgcggc actcattttc taccgactat ttcacctgcc
     6901 gatctcgctt ataaaagtgt ggcgtccaat ttaagcgatc ttgccgcaat gggtgcagaa
     6961 cctgcatgga tctctttagc cttaacctta cctgaaatta atcacgattg gctagctgaa
     7021 tttagtgaac atttctttga gattcttgat cactataatg ttgatttgat tggaggggat
     7081 actacttgtg gaccactatc tttgacaatc acagctcaag ggattgtgcc acaagataga
     7141 ggcttattcc gccataatgc taaagtaggc gattggatct atgtttccgg cacactcggt
     7201 gacagtgcag gtggtttgca acttctgcta aatcagcaaa gcgagcaagt gaattggaat
     7261 aaagagcaaa gctacctgat tcaacgccat ttacgcccaa cacctcgagt tttacttggc
     7321 ttgactatcg cctctattgc taatgcagcg attgatattt cagatggtct attgtctgat
     7381 ttaagccata ttttaaagcg cagtgactgt ggtgcagtat taaaccttga gaatttcccc
     7441 ttgtctgacg tcttacttag ttgttattct cgaacagagg cagaatattt tgccttaaac
     7501 ggtggcgaag attacgaact ctgctttacc gtgccaaatg acaacaaagc caaattagac
     7561 aaagcattag ccaatatcgg agtaacttat acatgtattg ggcaaattcg ttcggctaaa
     7621 gaaggtttaa ccctattacg tcataatcag cctgtcacct tgccctcaca acgaggctac
     7681 gatcatttta aaaaataacg caatgaaacc aattaatctt aaaaatccga ttcatttttt
     7741 tgcctttggc tttggttcag gcttattaaa gcctgctccc ggcacttggg gtagcctcgc
     7801 cgggctaatt gtagcaatag ccttgtggtc tatcacacaa tccgccctgt ttttcggctt
     7861 actcacccta gtttctttta ttgccggttg tgttatttgc accaaagcca gccaagatct
     7921 agaagtacac gatgacgggc gaattgtttg ggacgaaata gttgccatct ttttgatgtt
     7981 cacctgtttg cctgaataca atcttcttgg ctacctgctc gcctttatca gctttcgcat
     8041 ttttgatatt ttgaagcctt ttccaatccg ctattttgat gaaaaattag aaagcggttt
     8101 aggcattatg tttgatgata ttctcgcagc catttttgcc ctgatttcac ttcatctttt
     8161 atattatttc atttaaaaat ttatgctcac tattttattt attcattttg ccgggcttat
     8221 cagccccggc cccgatttct tctatgtcgt cagacaatcc gccagccact cagttaaaag
     8281 tggggtactt gccgcaaccg gaatctcaat cggcattatt ttttgggcag cctttgcgat
     8341 ttttggttta gcgtggctca gtaatagtat tggctcgctg tttcaatatg ccattatgat
     8401 tatcggtggc tgttacctgg tttatattgg ctctcaaatg gtacgggtaa ccgaaaatat
     8461 catttttgtt gaaaataaag ctcaactcac gccgctaaac ccttggcagg aaattaaaaa
     8521 aggcttatta atcaatatct ttaatgccaa agctggcgtt tactttacca gcgtcatttc
     8581 agcctatgta gataatttaa gcaataccct acaaatgttc gaactacttc tactctttgt
     8641 ggtaagtacc tttatttatt tctgtgtggt ggcactactc ttttctcgcc gaccggtcag
     8701 actgttctat gccaaatata gtcgctatat tgacaatatc tcaggcgtaa ttttcatctt
     8761 attcggctcg atgctgattt atgaaggatt cagccacttt atctaatcac aagcggtctg
     8821 atttcactaa aaatttgcaa attccgctat caataaatag tgttatttta tacagtttct
     8881 gctatgctac tgccaatttt aactctgaga gaacaaaatg attggacgat tacacggcaa
     8941 aattattgaa aagcaaccgc ctgaaatttt attagacgtg caaggggtag gctatgaact
     9001 actgctcccg atgaccagtt tctacagcct gccacaaatt ggcgaagagg cgactatttt
     9061 cacccattta gtggtgcgag aagatgctca cttgctattc ggctttgccc aaaaacaaga
     9121 tagaactcta ttccgtgaat taattaaaac caacggtgtc gggccgaagc tcgcgttggc
     9181 aattctatca gcaatgtcgg tgtcgcaatt tgccaccgcg attgaaaatg aagagctggc
     9241 aaaattaacc aaaattccgg gaatcggtcg gaaaaccgca gaacgcttat tggtggaact
     9301 caaaggcaaa tttaaaagaa tggcacagag tgatttcttt gtggaagtat cgcacgagca
     9361 aattgtggca agtaatacgc cagacccggc aaatgaagcc cttgatgcct taatagccct
     9421 aggctacaaa cctgccgatg cggaaaaaat gattaagaaa gtgaataaat ccggtgcaac
     9481 gagcgaacag cttattcgtg aggcgttaaa aaattcatta tgatattgat agtatagggc
     9541 gaaagatatt tcgcccctac aatctcattt ttaaaaatat atgtgtaggg gcaaatcaat
     9601 atttgccctc atgagtaata tagggtaata ccattccact acaacaataa tctaaatgta
     9661 tgattgaagc agatagaatc atcagtgcct cgcctaagcg tgaggaagaa gttatagacc
     9721 gagcaatccg cccgaaactg cttgccgact atgtcgggca gccttctgtg cgtgatcaaa
     9781 tggagatctt catcaaagcc gctaaattgc gggaagaggc attagatcac ttgctcattt
     9841 tcggcccgcc cggtttgggg aaaactaccc ttgccaacat tgtggcaaac gaaatggggg
     9901 tgaatattcg cacaacttca gggccggtgc tggaaaaagc aggcgatttg gcagcaatgc
     9961 tgaccaacct tgaaccttac gatgtgctgt ttattgatga gattcaccgc ctttctccag
    10021 cgattgaaga agtgctttat cctgcaatgg aagattacca gctcgatatt atgattgggg
    10081 aaggacctgc tgctcgttcc atcaagctcg atctaccgcc ttttaccctt gtaggggcaa
    10141 ccacccgtgc cggctcactg acctcaccgt tacgagatcg ttttgggatt gtacaacgct
    10201 tagaatttta ctcagtggac gatcttactc tgattgtcaa acgcagcgcc gattgcttaa
    10261 atctcaattt gtcggcagac ggtgcttatg aagtggctcg ccgctcccgt ggcacacccc
    10321 gtattgccaa ccgcttactc cgccgagttc gggattacgc cgatgtgcgt aataatggca
    10381 ttattacctc cgacattgcc aaacaagctc tggcaatgct cgatgtggat tcggaaggct
    10441 ttgattttat ggacattaaa ctgttgcaag cgattgtaga acgctttgat ggcgggccgg
    10501 ttggattaga caatcttgcg gcagctatcg gcgaagaacg agatacgatt gaagatgtgc
    10561 tagagcctta tctgattcag caaggctttc tacaacgcac accaagaggc agaattgcta
    10621 ccagcagaac ctatgcccat ttaggtataa cccaaataga ttagtcatat ttctaaaggc
    10681 attgatacat aaataaaaag cggatagtta gtactatccg ctttcatttg caaaaaatca
    10741 ataaaatttg accgcttatc cgctaggctt tttcagtctc aaaacgctca aaagctaata
    10801 ctctgctttc taattttctg agaacgattg tcataatgcc ggtaattacc aagtaaataa
    10861 cccctgccat accataaatt gataatgcgt catactctgt accataaagc tggcgagcat
    10921 agcccataat atccataatc gtaatggttg aagcaagcga tgtgccttta aaaactagca
    10981 caatctcatt actataagaa ggtaaagcac gttttaatgc gtaaggcact aaaattttca
    11041 gtgtatctaa acggctcaaa ccaagggctg cgcaagtttc ccattgcccc ttcggaatcg
    11101 cttttaccgc accgtggaac aacaatgtcg aataggcagc actgtttaac gctaaagcca
    11161 acattgcaca gaaccaagcg tcggataata atacccaagc cgcactttct gtaatccatt
    11221 caaattgccc cgggccacta taaatcaaga aaaattgaat taataaaggc gttccggtaa
    11281 aaagcgtaat aaatccgttt accaaccact ttaacggacg attttctaac gagagaataa
    11341 acgtaagaat gatcgctaaa ataaaggcaa tgatgagcgc cacaaaagtg agctgtaggc
    11401 tggtgccaat accctgtgaa atttcaacta aataatccca ccacattaag caactcctcg
    11461 ctcaaaacgg gtaaatctac cttctaatac tttaatcact cgctggctaa ttagagtaat
    11521 cgctaaataa ataagtgctg ctatcccata ccaagtaaaa ggttgatgag tattagcatt
    11581 aattaactcc gattgacgca ttaaatcatc taccccaatt aacgatacca atgctgtatc
    11641 ttttaacagc actaaccact gattgcttaa gcctggtaaa gcgtgtcgcc agacttgagg
    11701 gagaataatg cgaacaaata cttgcgctcg gcttaaccct aacgccgtac cagattccca
    11761 ctgcccaact ggaatagctt taattgcacc acgtaaagat tgggaagcat aagcagcaaa
    11821 aataataccc agcgcaatag ccccacacca gaaagcattt aactccgcaa actcatcggt
    11881 gatcaagaaa ataacttgga taagaccaaa atagatcaaa aataccacta aaatctccgg
    11941 caacccacgc aataatgtaa ttattatact agttggttta cggatacatg caatcttact
    12001 ggtttcaagt accacaaaaa agattgacaa taacattcca atcaagaggc ttgccagcgc
    12061 aatccctaag gtcatcaggg ttgcgttgac aagtaagttt aaaatatcgc tagacattat
    12121 ttcccagcca tccaagtatt ataaatttgc tcatatttac catttgtttt gatggtagct
    12181 aaagccgaat ttaatttttc aagcaatgca gtgttagatt tatttaccgc aataccatat
    12241 ccattaccaa aaaaggcttt atcgactact ttttcaccca cgaaatctaa ctctttttcg
    12301 gtttttagcc actctgccaa tactggtgta tcaccaaata ccatatcaac acgtccattt
    12361 tttaaatcta acactgctga ttgtaggcta gcgtaaggtt ttaaattata ttgtttggca
    12421 tttttgctta agtattgttg aaaagttgta ccattttgca cgccgacatt cttagctgtt
    12481 tccggtgtaa atttcccctt cattgcgaca taacttactg agttttctaa ataagcattg
    12541 gtaaaggcaa cctgtttttg acgcacttca gtaatatcca tgcctgaaat aattgcatca
    12601 aaatttttaa attttaatcc cgcaattaag ctatcgaatg attgcgtttt aaattcgcaa
    12661 gttgctttta gttcttcaca taatgctttt gcaatatcta catcaaagcc aataacttca
    12721 cctttttcat ttgttgtttc aaaaggggcg taagaaggct ccatcgcaaa agtgatagtt
    12781 tcttgtgcat tggcagaaag cgttgcaaat gcgatagcag aagctaataa taattttttc
    12841 attgttgtgt ccttttctgt tgttatagat tactctgagt gagataaata ttgagcaaat
    12901 tgctctgttt tcggtgattc aaaacaactt gcatcacctt gttcaataat tgtacctttt
    12961 tccatgtaca ccactttgct tgccacctta cgagcaacgc ctacttcgtg agtcacaatc
    13021 acctgagtaa tgcctgtaga ttgcaattct ttaataatat caactacttg tgcagtaatt
    13081 tccgggtcaa gtgctgcggt aggttcatca aataataaga cctgtggctg catcatcagt
    13141 gctctggcaa tcgccacccg ttgttgctga ccacctgaaa gatgcagtgg gaaacgctcg
    13201 gcaaactcgc tcaaacgcaa acgtgccaaa tgtccttttg ctctggcaac cgcttcttct
    13261 ttgcttaacc caagcacttt catcggggct tcaattaagt tttgcattac ggttaaatgc
    13321 ggccataaat gatactgctg gaacaccatt cccacctcac ggcgtaacaa actcaccgct
    13381 ttgctgtcgg ttttagcagt taaatcaaat tgatgatttg caatcgccaa agtgcctgat
    13441 tgcggtactt ccattaagtt taaggttcga attagggtac ttttacctgc cccactcggg
    13501 ccaagcaata caacagtatc ccctttttca atctctaagt taatgtcaaa taaggcttgg
    13561 tttgagccat aaaaaaaatt gacattttga atacgaattg ccattcgtta aaatcctact
    13621 acatttaaaa t
//
LOCUS       DANJWC010000052        13349 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.052, whole genome shotgun sequence.
ACCESSION   DANJWC010000052 DANJWC010000000
VERSION     DANJWC010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13349
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.052"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..297)
                     /locus_tag="PWX02_001123"
     CDS             complement(<1..297)
                     /locus_tag="PWX02_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014668079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMP-N-acetylneuraminate-beta-galactosamide-
                     alpha-2, 3-sialyltransferase"
                     /protein_id="HDL1729147.1"
                     /translation="MNLIICCTPLQVLIAEKIIEMHPNERFYGVMLSTVKNAKFDFYQ
                     ARLAQKCEQFFAMQQHSERIQLLKEIIYLKRTFGGKKFNKVFLANINELEIQFLL"
     gene            complement(294..1499)
                     /locus_tag="PWX02_001124"
     CDS             complement(294..1499)
                     /locus_tag="PWX02_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HDL1729148.1"
                     /translation="MKAVKDSVIYLIGELASKSIPFLLLPYFSRKLGVAGYGELSYYQ
                     TFAALFVIFIGLSQDGAVARYFYFYGKRSLNLVLSTGYLYTLCATLLILLGCWYFQSE
                     IIAYVAIGAMFQSFLNVQLSVRQCQKQALAYTTIQLMSAIFSAVITVAMMEYFKTDLV
                     EKRILAILMSNGLVFLIAYFLYAKRAKTKKFSLARHKTALLYLFGFGIPLILHHSSIF
                     LKGQLDRVFIYHKFSEEDLGLYAMGAQIAMILAIVVQALNKATLPYFYEALKQKKINV
                     SHIHKWALYALFIVPLPAIVMWFIPEELVVWILGSQFVGTKYYIILFLIATMLVVPYL
                     ILVNYLFFYGKNKLISLCSILSTIFYLVALFALTFTKIEYIPFASIIGAISIIPILYI
                     MTAKVGKTV"
     gene            complement(1509..2693)
                     /gene="neuC"
                     /locus_tag="PWX02_001125"
     CDS             complement(1509..2693)
                     /gene="neuC"
                     /locus_tag="PWX02_001125"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HDL1729149.1"
                     /translation="MKNIAYITGSRAEYGIVKRLLQKLAQDPTVNFSLVVTAMHLDPQ
                     YGNTVTVIGQDGFEIAARIPLTLNSENNQTIIHSMAECLDRFGEHFQQHRYDAVIVLG
                     DRYEMLAVATAAAMHNIPLIHLHGGEQTLGNYDEFIRHCITKMSKLHLTSTEEYRQRV
                     IQMGELPENVVNTGSLGAENSLDLVLPTQAELVERLGIPASPYFLVVFHPETLTDVSV
                     LEQIEALLNAFEQFKDKYQFVFIGSNSDTNSEQIYARFKAYTDENQFKFFTSVKPEEY
                     LALIKYSNGLIGNSSSGLIEAPSCGVGTLNIGNRQQGRVRGESVIDVESNEQAIIDGI
                     EKLISADFQHRLPSMKNPYFQDNAMEKAYNAIKIFLADSHHTQPKGFYDVAFSQAVKK
                     SE"
     gene            complement(2694..3953)
                     /locus_tag="PWX02_001126"
     CDS             complement(2694..3953)
                     /locus_tag="PWX02_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HDL1729150.1"
                     /translation="MKKIAIITARAGSKGLPNKNVLLANGKPLMAYSIEAAIESGQFE
                     KIIVSTDSQEYIDLLSHYPIEFIKRAEHLASDKASTFVVIEDVLNQYRSVEFDYFMLL
                     QPTSPLRSGEHIKESCEKFEKNFEQFDFLVSVSDAHKPTTLTRMIEEDESLKHFQLDY
                     SNYARQLHNPEYSPNGAIFTAKPEAYLKQRHFYGAKCLAYFMDKDVSIDIDDRQDFEY
                     FYFILQQRNKEKILLETIKRSILMKKENFKEVKEIALIGHSIIEYWDIEHLNGKAVNN
                     LGIAGISTKQYSDLILDEGLISALPKDIILMFGTNDIVYAGWSDEKILADINHLIAQL
                     TAIRGDINLYFLEITLTAFRVDRNNNDIRLLNQYLKDNLAVKWISLDQAFSDKYGKLD
                     LSYSDDGLHLNAKGYEKLTAILEKELN"
     gene            complement(4013..5050)
                     /gene="neuB"
                     /locus_tag="PWX02_001127"
     CDS             complement(4013..5050)
                     /gene="neuB"
                     /locus_tag="PWX02_001127"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HDL1729151.1"
                     /translation="MVNVFIVAEIGCNHNGDPALAKKMVDVAKECGVDAVKFQTFKAD
                     KLISKYAPKAEYQKVTTGTADSQLEMTRKLELPYEEFVKLEAYARSLGLEVFSTPFDF
                     ESIDFLASQQQKVWKIPSGELTNLPYLEKIARLPIEGKTIVISTGMATIEETKASLKV
                     LEDNGVAKKDITILHCNTEYPTPYEDVNLNAFHQLKQEFGDYSLGFSDHSAGYFAGLA
                     AVPYGITFIEKHFTLDKNFEGPDHKASVTPEELKLLCEGIRAVEKSLGSSEKLVTNSE
                     AKNKIVARKSIIAARPIKKGEIFTTENITTKRPGNGISPMFWYDVLGKEAQQDFEEDQ
                     LIQDSRFENQM"
     gene            complement(5050..5694)
                     /locus_tag="PWX02_001128"
     CDS             complement(5050..5694)
                     /locus_tag="PWX02_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL1729152.1"
                     /translation="MQQKSKREKLILIGAGGYAKSVLDSLDHEQYEFCGFIDNFKPEG
                     SVHLGYPILASTIDCFSKRDNYHYFICIGNNSYRLEKFSKLQKYKCRIINVVDKTALV
                     SKNSTLGIGVFVGKMAIVNSGVTVGDNVIINTKSLVEHGCFIGSHCNISTNTTLNGDV
                     IVEDHAFIGSSSVVNGQLRVGESALVGSGAVVIRNVEPRTVVAGVPAKYIKDIK"
     gene            5827..6570
                     /locus_tag="PWX02_001129"
     CDS             5827..6570
                     /locus_tag="PWX02_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL1729153.1"
                     /translation="MFEFIKLITAMILPPFNIILLWLLALILFSLGYKYAAYFFALLG
                     ILILYIFSIPYTSQKLGDSLVEAEPFTIEKYKNAQAIVVLGGGLRDSQELFGKLAITG
                     IPLERMRYAAYLHKETGLPILATGSSPNGTSEAKVMADEFKMFFNIETKWLENKAKTT
                     KENAIFSREILEKENIHKIILVTNQWHMKRAEMLFSQQGFEVMSASVGSGDTPDHYKL
                     NFMHFIPQAGAMNSNMLALKEWIGYWKEK"
     gene            complement(6647..8845)
                     /gene="priA"
                     /locus_tag="PWX02_001130"
     CDS             complement(6647..8845)
                     /gene="priA"
                     /locus_tag="PWX02_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDL1729154.1"
                     /translation="MALNVPLHRYFDYLLPPAFNACQGGRVVVPFGRQTKIGIVVDFP
                     QTSDVPTEKLKPIKAALDSSPIFDSEMWQLLNWAARYYHAPIGEVLTGALPIKLRNGD
                     STERVQSNYFVVTEQGRKSLIIGENKKAKKQVELLTELAECAKYFEKPTACSPSAWKG
                     LLEKGYIEKIDVPFMAQAWQDSLGEFPLVNTNNRLILNKQQTLVVSRLNAQTTFASFL
                     LNGVTGSGKTEVYLQLIETVLKREEQVLLLVPEIGLTPQTVQRFKARFNVEIDVLHSN
                     MNETERLNAWLRAKNGESAIVIGTRSALFTQFQKLGVIILDEEHDSSFKQQDGWRYHA
                     RDLAVVRAKNANIPIVLGSATPSLESLHNVQNGKFIELTLTARAGNAQLANQQIIDLK
                     TQRVTAGLSERLLSMMKTQLEQGNQVMLFLNRRGFAPVLLCHECGWVCECEACNKPFT
                     YHQKQKVLRCHHCATQRVIPRQCGHCGSTHLITTGVGTEQLEQVLNQQFPHYQVTRID
                     RDSTARKGALENHLTAIREGKSQILIGTQMLAKGHHFPNVTLVAIVNVDSALFSTDFR
                     AEEKLAQLYIQVAGRAGRAEKKGEVVLQTHYPDHPLLKTLLEQGYRAFADEALKMRQL
                     MSLPPFSSQVLFKATGKDNQQILQLLEQLADYFSQKIQQQNGQGFQLPPPFSAPMAKK
                     AGHYRWLLLIQHPLRGALQQLLDAFDQERESLNIPNNIRLNIDIDPQEIS"
     gene            complement(8857..9469)
                     /locus_tag="PWX02_001131"
                     /pseudo
     CDS             complement(8857..9469)
                     /locus_tag="PWX02_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818858.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
     gene            complement(9462..10359)
                     /gene="rarD"
                     /locus_tag="PWX02_001132"
                     /pseudo
     CDS             complement(9462..10359)
                     /gene="rarD"
                     /locus_tag="PWX02_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247891.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
     gene            complement(10586..11803)
                     /locus_tag="PWX02_001133"
     CDS             complement(10586..11803)
                     /locus_tag="PWX02_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL1729155.1"
                     /translation="MDRFPKSDKLAQVRYDIRGPIHKEALRLEEEGHKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIVQYYQSKGMRGMDVNDVYIGN
                     GVSELITMSMQALLNDGDEILIPMPDYPLWTAASTLAGGKAVHYLCDEENEWFPDLED
                     IKSKITPRTKGILVINPNNPTGAVYSRQILLEIAELARQHNLIIFADEIYEKIVYDGA
                     VHHHIAALAPDVLTVTYNGLSKAYRVAGFRQGWMVLSGPKHQAKGFIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFVLPGGRLLEQRNKMHELLVQIPGISCVKAKGALYM
                     FPKIDTEMYGIKDDQKFIYDLLQQEKVLLVQGSGFNWKKPDHFRVVTLPYAHQIEEAI
                     GRLANFLKTYRQD"
     gene            11991..13295
                     /locus_tag="PWX02_001134"
     CDS             11991..13295
                     /locus_tag="PWX02_001134"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HDL1729156.1"
                     /translation="MLIFHQLKQELTKSYSKVGREQGLVAIQASMPLLEANIPLLGWL
                     KTQKTSYPHFFLQYRDTDQTIATIGTLRQFYTLEAAQSFIQQHHFPLIGGLQFEGNTQ
                     FILPQFSLVKNQQNLTACFYFDSDNFEQQAVIFEQFLANFSQITELGLTDNPLVSMSA
                     TSHFEQWKANIEKAIISIEQGDFQKVVLANAMRLHFEQPICAYSLLAQSQKTNLGCYH
                     FLWAESADVAFLGSTPERLYQRQGLYFSTEALAGTAAVTTSEVQTELNAQWLLSDPKN
                     IYENQLVVDDIENQLKDCVTEFAVSEAKIKRLHNVQHLRRQIQATLKPDTSDSDCLSK
                     IHPTAAVAGLPRQKAKGFITENEPFTRHWYAGTLGVMGNEEAEFCVTLRSALISQNSI
                     TLYAGAGIVAGSDPQSEWQEIKRKAQGMAKLLDVDLVTKGEL"
BASE COUNT         4122 a         2847 c         2429 g         3951 t
ORIGIN      
        1 taacagaaat tgaatttcta actcattgat atttgctaaa aagactttat taaacttctt
       61 accgccaaaa gtacgtttta aataaataat ttcttttaat aattgaatac gctcgctatg
      121 ttgttgcatc gcaaaaaatt gttcgcactt ttgggctaat ctagcttggt aaaaatcaaa
      181 tttcgcgttt tttaccgtag aaagcatcac cccatagaat cgctcattag ggtgcatttc
      241 aatgattttt tcagcaatca acacttgcaa gggcgtgcaa caaataatta aattcatacg
      301 gtttttccta cttttgccgt cattatataa agaatcggaa taatgctgat tgcaccaatg
      361 atgctggcaa aaggaatata ttcaatttta gtaaaagtta aagcaaatag tgcaactaaa
      421 taaaaaatag tagataaaat cgagcaaagc gaaattagct tatttttgcc ataaaaaaac
      481 aaataattga ctaaaatgag gtaaggcaca accaacattg tcgcaattaa aaagagaata
      541 atgtagtatt ttgttcctac aaattggcta cccagaatcc ataccactaa ttcttccgga
      601 ataaaccaca tcacaatagc aggtaatggt acaataaata aggcatacaa cgcccatttg
      661 tggatatggc tgacattaat ttttttctgc tttaaggctt cataaaaata aggcagtgtt
      721 gctttattta aggcttgaac cacaatggct agaatcatcg caatttgagc ccccattgca
      781 tataaaccta aatcctcttc gctaaattta tggtaaataa atactctgtc taactgtcct
      841 tttaagaaaa tactggaatg gtgcaaaatc aacggaatac caaagccgaa taaatacaat
      901 aaagcagttt tatggcgggc aaggctaaat ttcttcgttt ttgctctttt agcatacaaa
      961 aagtaagcga ttagaaagac taagccatta ctcatcaaaa tcgctaaaat ccgcttttcc
     1021 actaaatcgg tcttaaaata ctccatcatt gcaacggtaa taactgccga aaaaatcgcc
     1081 gacattaatt gaatcgttgt ataagcaagt gcctgttttt gacactgcct tacactaagc
     1141 tgtacattca aaaacgactg gaacatcgcc ccaatcgcta cataagcaat aatttcagac
     1201 tggaaatacc agcaacccaa caggataagc aaagttgcac aaagagtata taaataccct
     1261 gtactcaaga ctaaatttag tgaacgctta ccataaaaat agaaatagcg agcaaccgca
     1321 ccgtcttggc taagcccgat aaaaattaca aataaagcgg caaaggtttg gtagtaggaa
     1381 agctccccat aacctgccac gccgagtttg cgtgaaaagt agggtaagag caagaaagga
     1441 attgactttg acgccaattc cccaattaaa taaatcacac tgtctttaac tgctttcata
     1501 gttgcaaatt attctgattt tttgaccgct tgcgagaaag caacatcata aaatcctttc
     1561 ggttgagtat ggtggctgtc tgctaagaat attttgattg cattgtaagc cttttccatc
     1621 gcgttgtctt gaaaatatgg atttttcata ctcggtaaac ggtgttggaa atcggctgaa
     1681 atcaattttt cgataccatc tatgatagcc tgttcattac tctctacatc aattacgctc
     1741 tcaccccgta ctcgcccttg ctggcgatta ccgatattta gcgtaccaac accacaactc
     1801 ggtgcttcaa tcaagcctga tgagctatta ccaatcaacc cattcgaata tttaatcaac
     1861 gctaaatact cttccggttt gactgaggtg aaaaacttaa attgattttc atcggtgtaa
     1921 gccttaaaac gtgcataaat ttgctctgaa ttggtgtctg aatttgagcc gataaagacg
     1981 aattggtatt tgtctttaaa ctgctcaaaa gcattgagta acgcctcaat ttgctccaac
     2041 acggaaacat cggttaaggt ttccgggtgg aagaccacta aaaaataagg cgatgccgga
     2101 atccctaatc tctcgactaa ttctgcttgg gtcggcaaca ctaaatccaa actattctcc
     2161 gcccctagag aaccagtatt caccacattt tcgggcagct cgcccatttg aatcacacgc
     2221 tgtctatatt cttcagttga agttaaatgc aatttcgaca ttttggtaat acaatggcga
     2281 ataaattcat cgtaattacc taatgtttgc tcaccaccgt gcagatgaat cagcggaata
     2341 ttatgcatcg ccgcagccgt tgccaccgcc aacatctcgt aacgatcgcc tagcacaatc
     2401 accgcatcat aacgatgttg ctggaaatgt tcgccaaatc gatctaaaca ctctgccatt
     2461 gaatgaataa tggtctgatt attttcactg tttaaggtaa gcggaatacg agctgcaatc
     2521 tcaaagccat cttgcccaat gaccgtcacc gtattgccat attgcggatc caaatgcatc
     2581 gcagtaacaa ccagagagaa attcacagtc ggatcttgtg ctaatttttg taataaacgc
     2641 tttacaatcc cgtattcggc acgagatccg gtaatatagg caatattttt catttagttg
     2701 agctcttttt ccaaaatagc ggttaatttt tcataacctt ttgcatttaa atgtaatccg
     2761 tcatcgctat agcttaaatc caatttgcca tatttatcgc taaatgcctg atctaagcta
     2821 atccatttca ccgctaaatt atctttaaga tattgattta acaagcgaat atcgttgtta
     2881 ttacgatcga ctctaaatgc agttagggta atttccaaga aatagagatt aatatctcca
     2941 cgaattgctg ttaattgtgc aattaaatga ttaatatctg ctagaatttt ttcatcactc
     3001 cagccagcat atacaatatc attagtaccg aacattaaaa taatgtcttt aggtaaagca
     3061 gaaattaaac cttcatccaa aatcagatca ctgtattgtt ttgttgaaat acctgcaatt
     3121 cctaaattat taactgcctt accgtttaaa tgttcaatat cccaatattc aatgatcgaa
     3181 tgcccgatta aggctatctc tttcacttct ttaaagttct cttttttcat taaaatcgaa
     3241 cgtttaatgg tttcaagtaa gattttttct ttattgcgtt gttgcagaat aaagtagaag
     3301 tactcgaaat cttggcgatc atcaatatca atagaaacgt ctttatccat aaaataggct
     3361 agacatttcg caccatagaa gtgtctttgt ttaagatagg cttctggctt agctgtaaaa
     3421 atagctccat tcggtgaata ttcaggatta tgcaattggc gtgcatagtt agaataatcc
     3481 aactgaaaat gctttaggct ctcgtcttcc tcaatcatac gagtcaatgt cgtaggttta
     3541 tgggcatcag aaacagatac taagaaatca aattgttcga aatttttctc aaatttttca
     3601 cacgattcct taatatgctc tcctgatctc aacggcgaag ttggttgaag tagcataaaa
     3661 tagtcaaact ctactgatct atattgattt aacacatctt caatcacgac aaaagtactc
     3721 gccttatcac tggctaaatg ctcggcacgt ttaataaatt caattggata atgtgagagc
     3781 aaatcaatgt attcttggga atcagtacta acgataattt tctcaaattg ccccgactca
     3841 atcgcagctt caatagaata ggccattaac ggcttaccgt tggctaataa tacattttta
     3901 ttcggcaagc cttttgaacc ggctcgggct gtaataattg caattttttt cataaatttg
     3961 accgcttgtt aaacacaaaa tgatggagct ttcatcccat cactatatta tattacatct
     4021 gattttcaaa acgagaatct tgaattaatt gatcttcttc aaaatcctgt tgtgcttctt
     4081 tacctagcac atcataccaa aacatcgggc taatgccatt accggggcgt ttagtcgtaa
     4141 tattttcggt tgtaaaaatt tcgccttttt taattggacg tgccgcaata attgattttc
     4201 ttgccacaat tttatttttt gcttccgaat tagtgactaa tttctcggaa gaacccagcg
     4261 atttttccac cgcacgaatg ccttcacaca gtaatttcag ctcttcaggt gtaaccgaag
     4321 ctttatgatc cggaccttca aagtttttat ctaaggtaaa atgtttttca atgaaggtaa
     4381 tgccgtaagg tactgccgcc aaccctgcaa aataaccggc agagtggtca gaaaaaccta
     4441 aactatagtc gccaaactcc tgttttagct gatggaacgc atttaaattc acatcttcat
     4501 aaggtgtcgg gtactcggta ttacaatgta aaatcgtaat atcttttttc gctacaccgt
     4561 tatcttccaa tacttttaat gaggcttttg tttcttcaat ggttgccata ccggtagaaa
     4621 taacgatcgt cttgccttca atcggaagtc gtgcaatttt ttctaaatac ggcaaattgg
     4681 ttaattcacc agacggaatt ttccacactt tttgttgttg gcttgccaag aaatcaatcg
     4741 actcgaaatc gaatggcgta gagaatacct ctaaccccaa tgaacgagcg taagcctcta
     4801 atttcacaaa ctcctcataa ggtaactcta atttgcgggt catttctaat tgggaatcag
     4861 cggtgccggt cgttactttt tgatactcgg ctttaggggc atatttagag atcaacttat
     4921 ccgctttaaa ggtttggaat ttcaccgcat cgacaccaca ctctttcgct acatcaacca
     4981 ttttctttgc taacgcagga tcgccgttat ggttacaacc gatttctgcc acaataaata
     5041 catttaccat tattttatat ccttaatata ttttgctgga acgcctgcta caactgtgcg
     5101 aggctccaca tttctaatta ccactgcacc ggagccaacc aatgcacttt cacccactct
     5161 tagctgtccg ttcacaacag aggaactacc aataaaagca tgatcttcga caattacatc
     5221 gccatttagt gtcgtattgg ttgaaatatt gcaatgactg ccgataaaac aaccatgctc
     5281 aaccagcgat ttagtgttaa tgatcacatt atcgcctacg gttacgccac tgtttacaat
     5341 tgccattttt ccaacaaaca ctccaatgcc tagagtggaa tttttggaca ccaatgcggt
     5401 tttatctacc acattaatta ttctacactt atatttttgt agcttagaga atttttctaa
     5461 acggtaacta ttatttccaa tacaaataaa atagtgataa ttatctcgtt tagaaaaaca
     5521 atcaattgtt gaagctagaa taggataacc taagtgcaca ctaccttcag gcttaaagtt
     5581 atcgataaat ccacaaaatt catattgttc gtgatcaagt gaatctaaca ctgatttggc
     5641 atagccaccg gcaccgatca aaatcaattt ttctctttta gacttttgtt gcattgctat
     5701 cacccatgat gaatttcaat tcatatggag tattctaata cttttcaaga tagaaaaata
     5761 gacaagtctt atataattta ggaaaatgaa catatcttta cttattttta ctacgatatt
     5821 ctttctatgt tcgagttcat taaactgatc accgcaatga tcctcccgcc atttaatatt
     5881 attttgctgt ggttgttagc attaatcctc ttttctttag gctataaata cgctgcttac
     5941 ttctttgcct tgttaggtat tctgatttta tatatcttca gcattcccta tacttcacag
     6001 aaattagggg attcattagt cgaagcagag ccatttacca tagagaaata taaaaatgct
     6061 caagcgattg tggtgctagg tggcgggcta cgagacagcc aagagttatt cggcaaactg
     6121 gcaattaccg gtattccgct ggaaagaatg cgttatgccg cttatctgca taaagaaacc
     6181 ggcttaccga ttttagcgac cggaagcagc ccaaatggga cttctgaggc aaaggttatg
     6241 gcagatgaat ttaaaatgtt cttcaatatt gagaccaaat ggctggaaaa taaggcgaaa
     6301 accaccaaag aaaatgcgat tttcagccgt gagattttag aaaaagagaa tattcataaa
     6361 atcattcttg ttaccaacca atggcatatg aaacgggctg aaatgctgtt tagccaacaa
     6421 ggatttgagg tgatgtctgc cagcgtcggt tctggcgata cgcccgatca ttacaaactc
     6481 aactttatgc actttattcc gcaagccggt gcaatgaata gcaatatgct ggcattaaaa
     6541 gagtggattg gttattggaa agagaaataa ccgaagataa atagcagcaa ggcaggtttt
     6601 cacctgcctt ctttttgcaa aaaatcagtc aaaaacaacc gcttgtctaa ctaatctctt
     6661 gtgggtcaat gtcgatattc agcctgatat tattcggaat gtttaaactt tccctttctt
     6721 gatcaaaggc atcgagtagc tgttgtaatg ctcctcggag agggtgttga atcagcaata
     6781 accagcggta atgtcctgct ttcttcgcca ttggggctga aaatggtgga gggagctgga
     6841 agccttgccc gttttgttgc tggatttttt ggctaaaata atctgctaat tgctcaagca
     6901 gctgtaagat ttgttgatta tctttgccgg tcgctttgaa caaaacttgc gaggaaaatg
     6961 gtggcaaact cattaattgc ctcattttta acgcctcatc agcaaaagct cggtagcctt
     7021 gttccagcaa ggttttaagc agtgggtgat cagggtaatg ggtttgtagc accacttcgc
     7081 cttttttctc cgctcgccct gctctgcctg ccacctgtat gtaaagctgt gctaattttt
     7141 cttccgcacg aaaatcagtt gaaaataacg ctgaatccac attaacaatc gccaccaacg
     7201 tcacattcgg gaaatgatgc ccttttgcca acatttgcgt accaatcaaa atttggcttt
     7261 tgccctctcg aatagcggtc aaatggtttt ccaatgcacc tttgcgagcg gtagaatctc
     7321 tgtctatacg agtcacttgg taatgaggga attgctgatt tagcacctgc tcaagttgtt
     7381 ctgtgccgac accggtagtt attaaatggg ttgaaccaca atgtccgcat tggcggggaa
     7441 ttaccctttg ggtggcacaa tgatggcaac gcaacacttt ttgcttttgg tggtaagtaa
     7501 aaggcttatt acacgcctcg cactcacaaa cccaaccgca ttcgtgacaa agtaataccg
     7561 gggcaaagcc acggcgattt aaaaacagca tcacttgatt gccttgctct aactgggttt
     7621 tcatcattga aagcaagcgc tccgacaaac cggcagttac ccgttgcgtt tttaaatcaa
     7681 taatctgctg attggcaagt tgtgcattgc cagcccgagc ggttagtgtc agttcgataa
     7741 atttcccgtt ttgcacattg tgcaaacttt caagacttgg cgttgccgag cccagcacaa
     7801 tcggaatatt tgcatttttc gccctaacca ccgccaaatc ccgtgcgtgg taacgccaac
     7861 cgtcttgttg cttaaacgag ctatcgtgtt cttcatctaa gataatcacg cccaattttt
     7921 gaaactgggt aaataaagca gagcgagtgc cgattacaat cgcactttct ccatttttcg
     7981 cccgcaacca cgcatttaac cgctcagttt cgttcatatt cgagtgcaat acgtcaattt
     8041 caacgttaaa gcgagcttta aagcgttgta ccgtttgcgg tgttagcccg atttcaggca
     8101 caagcagcag cacttgttcc tctcgcttaa gcaccgtttc aattaactgc aaatacactt
     8161 cggttttgcc cgagcctgtt acgccattga gtaaaaagct cgcaaaagtg gtttgtgcat
     8221 ttaaacggct gaccactaag gtttgttgtt tatttaagat cagccgattg ttagtattta
     8281 ctaacggaaa ttcacctagt gagtcttgcc acgcttgtgc cataaacggc acatcaattt
     8341 tctcgatata ccctttttcg agtaagcctt tccaagcaga tgggctacaa gcggtcggtt
     8401 tttcaaaata ttttgcacat tcggcaagct cggttaacag ttccacctgc tttttggctt
     8461 ttttattttc accgataatc agagatttcc gcccttgctc cgtcaccaca aaataattcg
     8521 attgaactcg ctcagtggaa tcgccattgc gtaattttat cggtaaggct ccggttagca
     8581 cttcaccaat cggggcgtgg taataacgag ccgcccagtt cagcagttgc cacatttctg
     8641 agtcaaaaat cgggctagaa tccaacgctg ctttaatcgg tttgagtttt tcggttggca
     8701 catcactagt ttgcgggaaa tcgaccacaa tgccgatttt ggtctggcga ccaaaaggca
     8761 caaccacccg cccgccttgg caggcattga atgccggagg tagcaaataa tcaaaataac
     8821 gatgtaacgg cacattcagt gccactctga ttaatctcac ttctctaaac caattttgat
     8881 tgcaagcccg ataaatatcg agccgcagat tttatttaac caatttccaa aggtttgatt
     8941 atgccgtaaa aaacgcccaa caggtgcagc ggctaagact aaaatactta cccaaataat
     9001 gctggtggat aaaaacgtta gccccaacag cataaaaggt attttccaaa ttactggata
     9061 caaactgcgg caataacgcc aagaaaaaga gcaatacttt cgggtttaat agattggtaa
     9121 tcaacccttg acagaatatc ttccatagag gaagtttgtc tagattgatt ttctctaaca
     9181 ccaacggctc tcgccacatt ttaatgccga gatagcagag ataaattgcc cctgcgtatt
     9241 tcagaaaatt aaataaagtg ggcgaatgtg ccacaatgcc tgcaagcccg agtgaaaccg
     9301 caagaatatg gcataaaatg cctgctagaa tgccgaatga agacattaag cccgactttt
     9361 tcccctcagc aatagtgcga gttaaaatat agatagtatc cgaacccggt gtaagattta
     9421 gcagaataca agcggtcaaa aatccccaat aatttacaat atcactcatt attcacactc
     9481 aatttacgtt tacgctgctg acgaagagca aacataccat ctaatactaa aaatgaaatg
     9541 gagatcaaca agcaaatcat caacaagtaa gattcatttg ctaacctttc gccaattaag
     9601 aaagacacca ccaacatcaa tagtggctca acatagccta ataaaccaag caaattaaag
     9661 ggtaaaatcg tgctggctag cgtatagctg ataagtgcag tgccactaat taaacccagt
     9721 aatgcaataa aatagtaaat attggggttt tgtgtttcga catattgcat atcggtttga
     9781 gagataaaat aacaagctac agggagcaaa tataaaattt ctaccacaaa gctatgtaaa
     9841 tggcttaagc caaactttcg gcgaaggtaa aaatagattg ggtaacctgt gcaaacaaat
     9901 aaacttgcta tcgaaatttt tcccgtaaac aaaatattac tcgctacacc aatgaaagca
     9961 accacaatcg ccaaccattt attcaaggaa agcgtttctt tataaaccac tttgccaaat
    10021 gccaccataa taatcggcat taatagatag cccattgaaa cctcaatcgc ttttccgcta
    10081 ttgggagccc ataaaaaccc acatctgccc gcccacaata gatgccgtga ttaaaataac
    10141 cagcaataaa taaggctctt gctttaggcg ttgaagaaat acccaaaact cattttgttt
    10201 tttaaataag tataaagcaa ggaaaagaaa aggaatcgta accagcatac gaatgccgaa
    10261 aatgctcaca cctgccagcg gttttagaaa gatcgccaaa tagtacatac agccgaataa
    10321 cactgaggct gaaagagata aaagaatgcc ttttaacata gtgataagaa agtaattatt
    10381 ttttaatttt tcaaatctcc cctacccctc ttttctaaag aggattgtcg agttctaaat
    10441 aaatgaataa tgttcggagg taggggcggg tccagtgccc gcccgcaaat taatatcgtt
    10501 agcggacagg cacaggacct gtccctactg atattataga acttacaacc ctgcttttat
    10561 aaagtggggt aaagggagat tcgacttagt cttgacgata ggttttcaag aaattcgcaa
    10621 ggcgaccaat cgcttcttca atttgatgtg cataaggcaa agttaccaca cggaagtgat
    10681 ccggtttttt ccagttaaaa ccggaacctt gcactaatag cactttttct tgttgcaaca
    10741 aatcgtagat gaatttttgg tcatctttaa tgccgtacat ttcggtatca attttcggga
    10801 acatatataa cgcccctttc gcttttacac agctaatacc cggaatttgt accaataatt
    10861 cgtgcatttt atttcgttgt tccagcaaac gaccgcccgg caacacaaat tcattgatac
    10921 tttgataacc acctaatgcg gtttgaatgg cgtgttgcat cggcgtattg gcacataaac
    10981 gcatagaaga gagcatatcc aacccttcaa taaaaccttt ggcttgatgc ttcggaccgc
    11041 tcagcaccat ccagccctga cggaaaccgg caacacgata cgccttggat aaaccgttat
    11101 aagttacggt taatacgtcc ggtgcaagag cagcaatatg gtgatgtacc gcaccatcat
    11161 agacaatttt ttcataaatt tcgtcagcaa aaataattaa gttatgctga cgggcaagtt
    11221 ctgcaatttc cagcaaaatt tgacggctat acactgctcc ggtcgggtta ttcgggttga
    11281 taaccaaaat ccctttagtg cgaggcgtga ttttcgattt aatatcttca agatcaggga
    11341 accactcatt ttcttcatcg cacaggtaat gcactgcttt accaccggct agggttgaag
    11401 ctgctgtcca taacggataa tcaggcattg gaattagaat ttcatcgcca tcatttaata
    11461 acgcctgcat cgacattgta atcagttcag atacgccgtt gccgatgtaa acatcgttta
    11521 catccatacc acgcatacct tttgattgat aatattgcac aatcgctttg cgggcagaat
    11581 acaaaccttt tgaatcacaa tagccttgtg cggttggaag attacgaatc acatccacta
    11641 aaatttcatc gggtgcttca aagccaaaag gggctgggtt accgatattt aattttaaaa
    11701 ttttatgccc ttcttcttct aagcgaagtg cctctttatg aattggccca cggatatcgt
    11761 aacgaacctg agctaactta tctgatttgg gaaaacgatc cattgtaaac ttcctataat
    11821 gtattatcta attgtagtct catcccgctg ttcaaaaaat cggtaattcg gaatgaattt
    11881 gtaaacatta ctctaatttg ggaggaaaaa aaagaggaaa tacgttaaaa tggagacctt
    11941 tttttcaatc agctaaaaac aagcggtttc tttttctgat ttttttgcaa atgcttattt
    12001 ttcatcaatt aaaacaagaa cttacaaaaa gttactcaaa agtgggaaga gagcaaggat
    12061 tggtcgccat tcaagcctct atgccgctat tggaggcaaa tattccgcta ctcggttggt
    12121 tgaaaacaca aaaaacaagc taccctcact tttttctgca atatcgtgat acagatcaga
    12181 ccattgccac cattggcaca cttcgccaat tttatacgct agaagctgca caatctttta
    12241 ttcaacaaca tcatttcccg ttaatcggcg ggcttcaatt tgaaggcaat acgcaattta
    12301 ttttgcctca attttcattg gtaaaaaatc agcaaaattt aaccgcttgc ttctattttg
    12361 atagtgataa ctttgaacaa caagcggtta tttttgaaca atttcttgca aatttcagcc
    12421 aaattaccga attaggacta acggataatc cgctcgtttc aatgagtgcc accagccatt
    12481 ttgagcaatg gaaagccaat attgaaaaag cgattatttc tattgagcag ggcgattttc
    12541 aaaaagtggt attggcaaat gcgatgagac ttcattttga gcaacccatt tgtgcttaca
    12601 gcttgctggc acaaagccaa aaaacaaatt taggttgtta ccacttttta tgggcggaaa
    12661 gtgctgatgt tgcctttctt ggctcaaccc ctgagcggct atatcaacgc caagggctat
    12721 acttttctac cgaagccctc gccggcaccg ctgcggttac caccagtgaa gtgcaaaccg
    12781 aactcaatgc acaatggctg ttaagtgatc ccaaaaatat ctatgaaaat caattagtgg
    12841 ttgatgacat tgaaaaccaa ctcaaagatt gcgtgaccga atttgcagta tccgaggcaa
    12901 aaattaagcg tttgcataat gtgcaacacc tacgccgcca aattcaagca acgctcaaac
    12961 cggacacctc agattcagat tgcttaagca aaatccaccc aactgcggca gttgcagggc
    13021 ttccccgcca aaaagcaaag ggttttatta ccgaaaatga gccttttacc cgccattggt
    13081 atgcaggtac attaggcgta atgggcaacg aggaagccga attttgcgta acactccgtt
    13141 ctgcattaat ttcacaaaat tcaattacgc tttatgccgg tgccggcatt gtggcaggct
    13201 cagatccaca atcggaatgg caagaaatta aacgtaaggc acaaggaatg gcaaaactgt
    13261 tggatgttga tttagtaaca aaaggggaac tgtaagctag actttcagaa cttaatctct
    13321 taattatttc tttctatatt ttcatttcc
//
LOCUS       DANJWC010000053        13310 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.053, whole genome shotgun sequence.
ACCESSION   DANJWC010000053 DANJWC010000000
VERSION     DANJWC010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13310
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.053"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(55..615)
                     /locus_tag="PWX02_001135"
     CDS             complement(55..615)
                     /locus_tag="PWX02_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729157.1"
                     /translation="MKKSFLSLLVLGTTMALAACDQAKEAAQSTTDKAVEVKEAVAEK
                     AAEVKSATEAKVEELKAAANDAAQATADKTEEAKKAVAEKATELKTTTEAKVEELKAA
                     ANDAAQATTDKTEEAKKAVAEKAAELKATTEAKVEELKAAASDATQATADKAEEAKKA
                     VAEKAAEVKVETETKVEEMKKAIESK"
     gene            complement(761..3703)
                     /gene="rne"
                     /locus_tag="PWX02_001136"
     CDS             complement(761..3703)
                     /gene="rne"
                     /locus_tag="PWX02_001136"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HDL1729158.1"
                     /translation="MKRMLINATQKEELRVALVDGQRLFDLDIESPGHEQKKSNIYKG
                     KITRVEPSLEAAFVDYGAERHGFLPLKEISREYFPSDYVFNGRPNIKDIIQEGQEVIV
                     QVSKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDERLELKEALDCLDV
                     PEDVGLIVRTAGVGKSPEELQWDLKVLLHHWEAIKKAAESRPAPFLIHQESDVIVRAI
                     RDYLRRDIGEILIDNKKVFEKAKNHIRLVRPDFINRVKLYEGEVPLFSHYQIESQIES
                     AFQREVRLPSGGSIVIDVTEALTAIDINSSRSTRGGDIEETALNTNLEAADEIARQLR
                     LRDLGGLIVIDFIDMTPVRHQREVENRIREATRQDRARIQFARISRFGLLEMSRQRLS
                     PSLSEASSHICPRCQGTGKVRDNESIALSILRLLEEEAIKENTAQVHTIVPVQIASYL
                     LNEKRKAISSIEKRHDVQIVVVPSESMETPHFSVYRLRENEITPTLSYDLTKHYEARN
                     EEEHPHHHHQEDVLVTRNEPVITVESVLQSSELNLQPSPTPTEPPKPSLLSRLIATIK
                     GWFSEEKVEEKPKPKARNQRDRRQRRERPNRNTKAENQERKTVQAQATPAEEKPVRSE
                     KKARRAVVEEVSGVTEQESRSNSREVQTERRQRRDLRKKVRVETQDVEAENLTPAVEE
                     TVAKQVENVQKNQQIQPLATQELPVETVPVVETTNEGRENNRQRRLPRHLRVGNQRRR
                     DNQRNQIASPMPLTSAVASPELASGKVWVQVTSTVKVEEKASFLSVDQMLEQQENQLV
                     ETEKKSEEKKLHTVSPLGGFVAQATNRDLVKEKQEQLSKPKEEKVASEDVKPVEVKEH
                     IAPFGSVVAQHSERDLVKEKNERVSKFQSQATVAPAIVEEVTNPIEVPKSTYESDYRF
                     NGRLGTFSRVEHTQAAMTIASAPSDKLEVVEISQWQNSKYYFYGKGFGGYTSAVSQVQ
                     SAPRSTNG"
     gene            complement(4160..5005)
                     /locus_tag="PWX02_001137"
     CDS             complement(4160..5005)
                     /locus_tag="PWX02_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL1729159.1"
                     /translation="MMSQEQFEEINQERRQALNHGSRIAMAASVLGALGIASTANAKQ
                     ASAEKSPYAEPEQYGLKRHGMTIDPKRVALVVVDPQIDFLSPKGVMWGVLGDSIKENN
                     TVANIESLFKAAKAVDMPTFVSSHYYYPTDHKWEFGSPGEHFMHDSGMFARKGQYTME
                     GFENSGADFMPEYKPYIFDGKTVIASPHKIFGPETNDLVLQLRKRKIDQVILAGMAAN
                     LCIDSHLRELIEQGFEVTVVKDATAGPRIPEGDGYLAALTNYRIIANDLWTTEEAVKK
                     MTAKA"
     gene            5132..5956
                     /locus_tag="PWX02_001138"
     CDS             5132..5956
                     /locus_tag="PWX02_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1729160.1"
                     /translation="MDSLTHLFTQFQFRTDLFHLGQLCQIGTFNEENKGYLHFVRQGR
                     FILNQAAERPITIERPAIIFSPTNILHSLHPLDDSGLDIFCINFDFGKGIRNPLTHTL
                     HNIVILELAQYPELGVLADQIFSEVNQKGCGYQAMIHHLCAYFTLKVARQCLEKGYIQ
                     TGLLKGLADKQLGKVLLAIHSAPERDWNVEELANVALMSRSRFAAYFKQIMGVSPMDY
                     LTNWRVAVAQHLLQKGIPVALVAEQVGYSHNAALSRVFMRELGLSPTEWLAQNKES"
     gene            complement(6040..7503)
                     /gene="ubiD"
                     /locus_tag="PWX02_001139"
     CDS             complement(6040..7503)
                     /gene="ubiD"
                     /locus_tag="PWX02_001139"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HDL1729161.1"
                     /translation="MKYKNLREFLDFLESQGELVRIQQEIDPYLEMTEIADRTLRKGG
                     PALLFENPKGYQMSVLCNLFGTPKRVALGMGQEDTKALRELGKLLAFLKEPEPPKGFK
                     DLIGQPPQWKQILNMPPKVLSKADCQQVVISGDEVDLYKLPIMHCWPGDVAPLVTWGL
                     TITQGPYKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDFQEWKAENPGKPFPVSVAI
                     GADPATILAAVTPVPDTLSEYAFAGLLRGHKTEVVKSISNELEVPASSEIVLEGYIDL
                     EETALEGPYGDHTGYYNEQEYFPVFTVTHITMRKDAIYHSTYTGRPPDEPAVLGEALN
                     EVFIPILQKQFPEIVDFYLPPEGCSYRLAVVTMKKQYAGHAKRVMMGVWSFLRQFMYT
                     KFVIVCDDDVNARDWKDVIWAITTRCDPARDTTLIENTPIDYLDFASPIAGLGSKMGI
                     DATNKWAGETSREWGTPIKKDDNVVKRIDEIWDSLGI"
     gene            7640..8614
                     /gene="znuA"
                     /locus_tag="PWX02_001140"
     CDS             7640..8614
                     /gene="znuA"
                     /locus_tag="PWX02_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HDL1729162.1"
                     /translation="MFKRTLIGLAVLGASVAQADVLTSIKPLGFIASAITEGVTETKV
                     LLPVTASPHDYSLKPSDVTQLQSAQLVVWVGDGLEGFLEKSIEKLPKEKVLTLEDVDA
                     IDDIVDKASKKEDKDDDHDHKHEHKHEHKHEHKHEGHNHEGHNHDHHGHSHDEDWHIW
                     LSSEASELAAEQIAARLSEQMPEHKAKIAENLAKFKANLTAKKAEIAKQLEPVKAKGY
                     YTFHDAYGYFEREYGLNSLGSFTINPTVAPGAKTLTAIKKNIAKNKAQCLFAEPQFTP
                     KVIETLAKETKVNVGQLDPLGAKVQLSATAYPEFLQVLADEFSQCLTK"
     gene            complement(8666..8986)
                     /locus_tag="PWX02_001141"
     CDS             complement(8666..8986)
                     /locus_tag="PWX02_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005621693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnA domain-containing protein"
                     /protein_id="HDL1729163.1"
                     /translation="MIYPTCPECKSENTYHDSIQFVCPDCAYEWTGEETDQDDDVLIV
                     KDSNGNLLSDGDDVLLIKDLKLKGSSEVLKKGTKFKGIRLAQGDHNVDCGKIMLKSEF
                     LKKA"
     gene            9127..11490
                     /gene="rnr"
                     /locus_tag="PWX02_001142"
     CDS             9127..11490
                     /gene="rnr"
                     /locus_tag="PWX02_001142"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDL1729164.1"
                     /translation="MIVDPNYQQELEKYENPVPSREFILNTIREHNAPMSRDELLSAF
                     HIDDEERMEGIRRRLRAMENDGELVFTKGKRYALPEKMDLIKGTVIGHRDGYGFLQVE
                     GHEKGATKSEDWFIPNAQMVKVMHGDFVLAQPNGTDRRGRKEVRIVRVLEARKKQIVG
                     RFFLESGIGFVVPDDSRVNQDILIPDEHRMGARMGQVVVVELQERKASFSRPVGIITE
                     ILGDNLAPGMEIEIALRNHDIPHIWPEGVEKQIRQFSSEEVPEEAKQGRVDLRDLPLI
                     TIDGEDARDFDDAVFAKKEGNGWRLWVAIADVSYYVRPKTALDLEALNRGNSVYFPNR
                     VVPMLPEVLSNGLCSLNPQVDRLCLVAEMSVSSKGELQDYQFYEAVMNSHARLTYTKV
                     WKMLEGDEALRERYAPLVPHIEELYAMFQTLMKARHKRGAIEFETIENQFIFNPQGRI
                     ERIEPLIRNDAHKLIEECMILANIAAARFVEKANEPALYRIHDKPSEEKLLSFKSFVR
                     ECGLTWDVGLDPTPKDYGVLLEQIAERADKELIQTMLLRSLKQAVYAADNIGHFGLAL
                     TEYAHFTSPIRRYPDLLLHRAIKYLIEKGKGNTRYYTDGGGYHYKLDDMDEFGDKCSA
                     TERRADEATREVADWLKCEFMQDHLGEEFDGVISSVTGFGLFVKLNELLIDGLVHIST
                     LDNDYYHYDADRQRLIGGSGIIYRLGDAVRVKVINVNLDEKKIDFELLTGNKKTGKTA
                     KKKAKATEVPVFKEPKTVKKAKKVAEKKPAKSAKKRTKTTASKSRKK"
     gene            11494..11760
                     /locus_tag="PWX02_001143"
     CDS             11494..11760
                     /locus_tag="PWX02_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729165.1"
                     /translation="MDYKKQILFSLLFALVIAIISYFLFTYIPFDCSDLSKLNDSQRL
                     ECRTAEVLGVYYWLPHTTTLFFGSVLLYWAVYGMISLVQAVLKR"
     gene            11983..12441
                     /gene="mraZ"
                     /locus_tag="PWX02_001144"
     CDS             11983..12441
                     /gene="mraZ"
                     /locus_tag="PWX02_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDL1729166.1"
                     /translation="MFRGASSISIDSKGRIAIPTRYRAELLEKHHGILVCTVDIRQPC
                     LLLYPLHEWEMVEQKLLALSNFDPVQRRIQRVMQGFATECEMDSAGRILLSPTLRQHA
                     QLEQQIMLVGQLNKFEIWQDKQWQAQIAEDLAFGSSAEILDCEALKNLSL"
     gene            12457..>13310
                     /gene="rsmH"
                     /locus_tag="PWX02_001145"
     CDS             12457..>13310
                     /gene="rsmH"
                     /locus_tag="PWX02_001145"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDL1729167.1"
                     /translation="MSENPKHITVLLHEAVDGLAIKPNGIYIDGTFGRGGHSRLILSK
                     LGENGRLIATDRDPRAIAEAQTISDPRFQIEHTAFSAIPELCERMGLVGKIDGILLDL
                     GVSSPQLDEAERGFSFMRDGPLDMRMDTTKGLSAAEWLAQVSAEDLAWVLKEFGEERF
                     AKRIAQAVVSYNKSANEKISRTLQLAQIIADAVPFKDKHKHPATRSFQAIRIFINSEL
                     DELEKALNSALTVLAPEGRLSIISFHSLEDRMVKQFVRKHSKGMDVPRGLPILEAELT
                     KNIPLKTIG"
BASE COUNT         3656 a         2781 c         2898 g         3975 t
ORIGIN      
        1 aatccaggct ttatttttag cgtgtttcga tagctacaga gaaactaaaa agcactattt
       61 gctttcaatc gcttttttca tttcttcaac ttttgtttct gtctcaactt tcacttctgc
      121 tgccttctca gctactgcct ttttagcttc ttctgcttta tctgctgttg cttgagtcgc
      181 atcacttgct gctgctttta gttcttccac tttagcttca gttgtggctt ttaactcagc
      241 agctttctct gctactgcct tttttgcctc ttctgtttta tctgttgttg cttgagctgc
      301 atcatttgct gctgctttta gttcttccac tttagcttca gttgtggttt ttaactcagt
      361 ggctttctct gccactgcct tttttgcctc ttctgtttta tctgctgttg cttgagctgc
      421 atcatttgct gccgctttca actcttcaac cttcgcttca gtagccgatt taacttctgc
      481 agctttttca gctacagctt cttttacttc aaccgctttg tctgtagttg attgcgcggc
      541 ttcttttgcc tgatcacaag cagcaagtgc catagttgta cctaacacta ataatgataa
      601 aaatgatttt ttcataccga gttccttaaa taaactaaaa ataacgcact ttgtaataaa
      661 gtgcgtttac ttaggcagaa aaattgatag aaagttcata ctttgataaa aaaatgccta
      721 ttagttataa ctaataggca tttaaaccaa ttaattgtac ttaaccattg gttgaacgag
      781 gcgctgactg cacttggctc actgcgctgg tatatccgcc aaaccctttg ccatagaaat
      841 agtatttaga gttttgccat tggctaattt cgactacttc aagcttgtca cttggtgcag
      901 aagcaatggt catagcggct tgggtatgct caacacgaga gaatgtacct aatctgccgt
      961 taaatcgata atcgctttcg taagttgatt ttggcacttc aatcgggtta gtaacctctt
     1021 ccacaatcgc tggtgcaacc gttgcttgcg attggaattt actgactcgc tcatttttct
     1081 ctttaactaa atcacgttca gaatgctgag caacaacact accaaatgga gcaatatgct
     1141 cttttacctc tactggtttt acgtcttcag aagcaacttt ttcttctttc ggtttggaaa
     1201 gctgttcttg cttctcctta accaaatcac ggttagttgc ttgtgctaca aagccaccta
     1261 gcggactaac tgtatgaagc ttcttctctt ctgatttttt ctctgtctca acaagctgat
     1321 tttcttgctg ttcaagcatt tgatcaacag ataagaatga tgctttttcc tctactttga
     1381 cagtagaggt aacctgaacc caaactttac cgctggcaag ctccggcgaa gcaaccgctg
     1441 atgtcagagg cataggcgaa gctatttgat tacgttgatt atcacgacga cgttgattac
     1501 ccacacgcaa atggcgaggt aaacggcgtt ggcggttatt ttcacgtcct tcgttcgttg
     1561 tttctaccac aggaactgtt tcaaccggca actcttgagt tgcaagcggt tgaatttgct
     1621 gatttttttg cacattttca acttgcttcg caacggtttc ttcaactgct ggcgtgaggt
     1681 tttctgcttc cacgtcttgt gtttcaacac gcactttttt gcgtaggtcg cgacgttggc
     1741 ggcgttcggt ttgaacctca cgagagttgc tacgactctc ttgctcggtt acgccggaaa
     1801 cttcttccac taccgctcgg cgtgcttttt tctcgctacg caccggtttt tcttccgccg
     1861 gagtagcttg cgcttgcacg gttttacgct cttggttttc cgctttggta ttgcggttcg
     1921 ggcgttcacg gcgttggcga cgatcacgct ggttgcgtgc tttcggcttc ggtttttctt
     1981 ccactttttc ttcgctaaac cagcctttaa tggtcgcaat taagcggctt aatagcgatg
     2041 gttttggtgg ttcggtaggt gttggcgaag gttggagatt cagctccgag ctttgtaaca
     2101 cagactctac cgtaatcacc ggctcgttac gggtaaccag cacatcttct tgatggtgat
     2161 gatgtgggtg ttcttcctca ttgcgagctt cataatgctt ggttaaatcg tagcttaacg
     2221 ttggggtaat ttcattttca cgcaggcgat atacgctgaa atgcggtgtt tccatacttt
     2281 cagacggtac tacaacaatt tgtacatcgt ggcgtttttc aatgctggaa atcgctttac
     2341 gtttttcatt gagtaagtag ctggcgattt gcaccggcac aatggtgtga acctgtgcag
     2401 tgttttcttt gattgcctct tcttctaata aacgtaaaat tgacagtgcg attgattcat
     2461 tatcacgtac tttacccgtt ccctgacagc gagggcagat atgcgatgaa gcctcactta
     2521 atgatgggct taaacgctga cggctcattt ctaacaaacc gaaacgggaa atacgggcaa
     2581 attggattct cgcacggtct tggcgggttg cctcacgaat acggttttcc acttcgcgtt
     2641 ggtggcgaac cggcgtcata tcgataaagt cgatcacaat caaaccgcct aagtcacgta
     2701 aacgtaactg acgagcaatt tcatcggctg cctctaagtt agtattgagt gcagtttctt
     2761 caatatcgcc accacgggtc gagcgtgaag agttaatatc aatcgcggtt aaggcttctg
     2821 tgacatcaat gacgatagag ccaccggaag gtaaacgcac ttcacgttgg aacgccgatt
     2881 cgatttgcga ttcaatttga tagtggctga atagtggtac ttcgccttcg tataatttca
     2941 cacggttgat gaaatccggg cgaacaaggc gaatgtggtt tttggctttc tcgaacactt
     3001 ttttgttgtc gattaagatt tcgccaatat cacggcgtag atagtcacga attgcacgca
     3061 caatcacgtc actttcttgg tgaattaaga acggagcagg gcgagattcc gccgcttttt
     3121 taatcgcttc ccagtggtgt aacaagactt ttaagtccca ttgtaactct tccggcgatt
     3181 tgccgacacc ggcggtacgc acgattaagc cgacatcttc cggcacatct aagcagtcta
     3241 atgcctcttt gagttctaaa cgctcatcgc cttcaatacg gcgggaaata ccaccggcac
     3301 gagggttgtt cggcattaaa actaagtaac tgcccgcaag cgaaataaaa gtggttaagg
     3361 cagccccttt gttgccacgc tcttctttgc tgacttgaac aatcacttct tgcccttctt
     3421 gaataatatc tttgatattc gggcgaccgt taaacacata gtctgacggg aagtattctc
     3481 gggaaatttc ttttaaaggt aggaagccgt ggcgttctgc accgtaatct acgaatgcag
     3541 cttcaaggct tggttcgaca cgggtaattt tacccttgta gatattggat tttttctgtt
     3601 cgtgacccgg gctttcaatg tccaagtcaa ataagcgttg cccatcaacg agggcaacac
     3661 gcaactcttc tttttgagtt gcattgatta acattctttt cattgtgttt tctcattatt
     3721 ttgtttttgt cttacacggt cattttgacc cattaataag ggaatggatt taatgtctat
     3781 ctcacgactg tcttcattcc cgatagtgca ttgatggttg tcttacgttt actttgtctt
     3841 gtcttaaaga aactgcaaaa tcaatcactt aaggcttgtt tatctctatt acaagcggtt
     3901 atttttgctt tattttttgc aattttctat tgctttttca agccaaaaat aacgagtcat
     3961 tatcgcattt taaccctgaa aaaggcaagg taattgttgt atggtagctg ttatttgttg
     4021 tggagatgaa tataatgaaa atgaatgggg tgataaggaa aaataatgag atttagtgtg
     4081 agtgctggag ataagcgtga aatccaagcg gttaaatttg caaaaaaaga gctaaattta
     4141 accgcttgta aagcgaatat tatgcttttg ccgtcatttt tttcactgct tcttcagtgg
     4201 tccagaggtc atttgcgata atacggtagt tggttaatgc tgcaaggtag ccgtcacctt
     4261 caggaatacg aggccctgcg gttgcatctt tcaccactgt tacttcaaaa ccttgttcga
     4321 ttaattcacg caagtgtgaa tcaatacata agttggcagc cataccggct aaaattactt
     4381 ggtcgatttt acgtttgcgt aattgcagca ctaagtcatt ggtttccggt ccaaagattt
     4441 tgtgtggaga ggcgatcact gttttgccat caaaaatata tggcttgtac tcaggcataa
     4501 agtctgcacc tgaattttca aagccttcca tggtgtattg accttttctt gcaaacattc
     4561 cgctgtcgtg cataaagtgt tcgcccggtg aaccaaattc ccatttgtga tcggttgggt
     4621 aatagtagtg tgacgaaaca aaggttggca tatctactgc ttttgctgct ttaaataaac
     4681 tttcaatatt cgctaccgtg ttgttctctt tgattgaatc acctaacacg ccccacatca
     4741 cgcctttcgg gcttaggaaa tcaatttgtg gatcaactac cactaatgcc acacgttttg
     4801 gatcaatagt cattccatgg cgttttaaac cgtattgttc cggctcggca taaggtgatt
     4861 tttctgcact ggcttgtttt gcatttgctg ttgatgcaat acctaatgca cctaatacac
     4921 tggctgccat tgcaatacga gagccgtggt taagggcttg acggcgttct tggttgattt
     4981 cttcaaattg ttcctgtgac atcattttat cctcattaat agatatacaa attgtaatga
     5041 taacttgcgt taccgaacgg gttgctatta taatgactcc cttccgcttg ttttaaccgt
     5101 tcgtctcaat tgtttaccca aggagcttaa tgtggatagt ttgactcatc tttttaccca
     5161 atttcagttt cgtacggact tatttcatct aggtcagttg tgccaaatcg ggacttttaa
     5221 tgaagagaat aaaggctatt tacattttgt tcgccaagga cgttttattt taaatcaggc
     5281 tgctgaacgc ccgattacga ttgaacgccc agcgattatt ttttcaccga ccaatatttt
     5341 gcacagtttg cacccgttag atgatagtgg gttagatatc ttctgcatta attttgattt
     5401 tggcaaaggg attcgcaacc cattaacgca taccttgcac aatattgtga tcttagagtt
     5461 ggcacagtat ccggaattgg gggtattagc cgatcaaatt tttagtgaag taaatcagaa
     5521 agggtgtggt tatcaggcaa tgatccacca tttatgtgcc tattttacct tgaaggttgc
     5581 tcgtcaatgt ttagaaaaag ggtatattca aacaggtttg ttaaaaggct tggcagataa
     5641 gcaactgggg aaagtgctgt tagctattca cagtgcaccg gaacgagatt ggaatgtaga
     5701 ggagttggca aatgtggcat taatgtctcg ctcacgcttt gctgcttatt ttaaacaaat
     5761 tatgggggtt tcaccgatgg attatctcac taactggcga gtagcggttg cccagcattt
     5821 attacaaaaa gggataccgg tagcattggt ggcagagcaa gtcggttata gccataatgc
     5881 tgctctcagc cgtgtcttta tgcgggaatt agggctttcg cctaccgagt ggttagctca
     5941 aaataaagaa tcgtaacctg attggcaata aagagcagaa tggtaaaaat atcagaaatt
     6001 taaccgcttg ttatgttgca caagcggtta aatgttgcct taaatgccaa gagaatccca
     6061 aatttcatca atgcgtttca ccacattgtc atcttttttg attggcgttc cccactctct
     6121 tgaggtttca cccgcccatt tattggtggc atcaatcccc atttttgagc caagtccggc
     6181 aatcggtgag gcaaaatcca gatagtcaat aggggtattt tcgatgagtg tggtatctcg
     6241 tgccggatcg cacctagtag taatcgccca aatcacgtct ttccaatctc gggcattcac
     6301 atcatcatcg cacacaatca caaacttggt gtacataaat tgacgtagga acgaccaaac
     6361 ccccatcatc acccgtttgg cgtgtccggc atattgtttt ttcattgtca ccaccgctaa
     6421 acggtaagaa cagccttcag gtggcaagta gaaatcgaca atttccggaa actgtttttg
     6481 caaaatcgga ataaagactt cgtttaacgc ctcgcctaac actgccggtt catcaggtgg
     6541 tctgccggtg taagtggagt gataaatcgc atcttttcgc atagtaatat gggtaacggt
     6601 aaataccgga aaatactctt gctcgttgta gtagccggtg tgatcgccat aagggccttc
     6661 caatgcggtt tcttccagat cgatatagcc ctccagcaca atttcgctac ttgccggcac
     6721 ttctaactca ttggaaatag atttgaccac ttcggtttta tgccctcgca acagccctgc
     6781 gaacgcatat tccgaaagcg tatccggcac aggggtgact gccgctaaaa tagtggctgg
     6841 atctgcacca atcgcaaccg acaccggaaa cggtttaccc gggttttcgg ctttccactc
     6901 ttggaagtcc aacgcaccac cacggtggga aagccaacgc ataatcagtt tgtttttgcc
     6961 gataagttgc tgacgataaa tgcctaaatt ctggcgtttt ttgtaaggcc cttgcgtaat
     7021 cgtcagcccc caagtgacca gcggagcgac atcacccggc caacagtgca taatcggtaa
     7081 tttgtagaga tcaacctcat cacccgaaat caccacttgc tgacaatcgg ctttgctcag
     7141 taccttaggc ggcatattta aaatttgctt ccattgtggc ggttgcccga tcaaatcttt
     7201 aaaccctttc ggtggttcag gctcttttaa aaaggcaagt aacttgccca gctcacgcag
     7261 agctttggta tcttcttgcc ccattcccag agctacccgt ttgggcgtgc cgaataaatt
     7321 acacaacacc gacatttgat agcctttcgg gttttcaaac agtaatgccg gcccgccttt
     7381 gcgtaaggtg cggtcggcga tttctgtcat ttctaaataa ggatcgattt cttgctgaat
     7441 acggactaat tcaccctgag actcaaggaa atcgagaaat tcacggagat ttttatactt
     7501 catagcggtc atatttttcg atttttttgc aagtatatag aaaaacgtaa atgaaaaata
     7561 cccataaaaa atttatagaa attttgtttt attttgttag aatagctcgg ttcaaattaa
     7621 atgccataag gggaatttta tgtttaaaag aacattaatc ggtctagccg tgttaggtgc
     7681 aagtgttgct caagcggatg tattaaccag tattaagcct ttaggcttta ttgccagtgc
     7741 cattaccgaa ggggtaactg aaactaaagt attattaccg gtaactgcct ctccacacga
     7801 ttacagctta aaaccttcag atgtgactca attacaatct gctcaattag tggtttgggt
     7861 gggtgacggc ttggaaggct ttttagaaaa aagtattgaa aaactgccga aagaaaaagt
     7921 attaaccctt gaagatgttg atgcgattga tgatattgtc gataaagcaa gcaaaaaaga
     7981 agataaagac gatgatcacg atcataaaca tgaacataag cacgaacaca agcacgagca
     8041 taaacacgaa gggcataatc acgaaggaca taaccacgat catcacggac atagccacga
     8101 tgaagattgg catatttggc tctcaagcga ggcaagtgag ctggcagcag agcaaattgc
     8161 ggcacgttta agcgaacaaa tgcctgaaca caaagcgaaa attgcggaaa atcttgcaaa
     8221 atttaaagca aatttaaccg ctaaaaaagc cgaaattgcg aaacaattag agccggtaaa
     8281 agccaaaggt tactacacat tccacgatgc ttacggctat tttgaaagag aatatggttt
     8341 aaattcattg ggttcattta ccattaaccc aactgttgcc ccgggtgcaa aaacacttac
     8401 agcaatcaag aaaaacattg cgaaaaacaa agcacaatgt ttatttgccg agccgcaatt
     8461 tacgccgaaa gtaattgaaa ccttagcaaa agaaactaaa gtaaatgttg gccaattaga
     8521 cccactcggt gcaaaagttc agttaagtgc gactgcttac cctgaatttt tacaagtttt
     8581 ggctgacgaa tttagccaat gtttaacaaa ataagttaaa aatcccccat ttaaatataa
     8641 atgggggatt ttgcgattac atcacttaag ctttttttaa gaattctgat ttcaacataa
     8701 ttttaccgca atcgacatta tggtcacctt gagctaaacg gattcctttg aattttgtac
     8761 cttttttcag tacttcagac gagcctttta attttaaatc tttgattaat aatacatcgt
     8821 ccccgtcaga taataaattg ccattactgt cttttacaat tagcacatca tcatcttgat
     8881 ccgtttcttc gcccgtccat tcatacgcac aatccgggca aacaaattga attgagtcgt
     8941 ggtaagtatt ttcgctttta cattcagggc aggttgggta aatcatttta attcctcaaa
     9001 ttaataaaga accagattat ataccgaaat ccagcaaaac gctaagcggt catattttgc
     9061 cgatttttta ccaatcgagc taaaagtcag ttagaatagc cttaattaca caaaaaggag
     9121 gatactatga tagtcgatcc gaattatcag caagaattag aaaaatacga aaatccggta
     9181 ccaagccgag agtttatttt aaatacaatt cgtgaacata acgccccaat gagccgtgat
     9241 gaattattgt cggcttttca tattgacgat gaagaacgaa tggaaggcat tcgccgccgt
     9301 ctgcgtgcaa tggaaaatga cggtgaattg gtgttcacca aaggcaaacg ctatgcgttg
     9361 ccggaaaaga tggatttaat caaaggtaca gttatcggcc atcgtgacgg ttacggtttt
     9421 ttgcaagtgg aaggacacga aaaaggggcg actaaaagcg aagattggtt tattcccaac
     9481 gcccaaatgg taaaggtaat gcacggtgac tttgtgctgg cacaaccaaa cggcacagac
     9541 cgcagaggtc ggaaagaagt ccgcattgtg cgagtgttgg aggcccgtaa gaaacagatt
     9601 gtcgggcgtt ttttccttga aagcggtatc ggttttgttg tgcctgatga cagccgcgtt
     9661 aaccaagata ttctcattcc ggatgaacac cgtatggggg cgagaatggg gcaagttgtg
     9721 gtggtggaat tacaggaaag aaaagcgagt tttagccgcc cggtgggaat cattactgaa
     9781 attctaggcg ataatttagc gccgggaatg gaaattgaaa ttgccctacg caatcacgat
     9841 attccgcaca tttggccgga aggggtagaa aaacaaatcc gccaatttag tagcgaagaa
     9901 gtgccggaag aagccaagca agggcgggtg gatttgcgag atctgccact gattaccatt
     9961 gacggcgaag atgcccgaga tttcgatgat gctgtatttg ccaaaaaaga gggcaacggc
    10021 tggcggttat gggtggcgat tgccgatgta agttattatg ttcgcccaaa aaccgcatta
    10081 gatttggaag ccctaaatcg tggtaactcg gtctatttcc cgaatcgggt tgtgccaatg
    10141 ttgccggaag tgctttccaa tggcttgtgt tcactcaatc cacaggttga caggctctgc
    10201 ttggtggcgg aaatgtcggt ttccagcaaa ggggaattac aagattatca attctacgaa
    10261 gcggtaatga actctcacgc ccgtttaact tacactaaag tgtggaaaat gttggaaggt
    10321 gatgaagcct tgcgtgaacg ctatgctcca ttagtgccgc atattgaaga actctatgca
    10381 atgttccaaa ccttaatgaa agctcgccat aaacgagggg cgattgaatt tgaaaccatt
    10441 gaaaatcaat ttattttcaa tccgcaaggg cgaattgagc gaattgagcc gttaatccgc
    10501 aacgatgctc ataaattgat tgaagaatgt atgattttag ccaatattgc tgccgctcgt
    10561 tttgtggaaa aagcgaacga accggcgtta tatcggattc acgataagcc gagtgaagaa
    10621 aaattgctca gttttaaatc ctttgtgcgt gagtgtggct taacctggga cgtaggctta
    10681 gatccaaccc caaaagatta cggtgtacta ctggagcaaa ttgccgaacg tgccgataaa
    10741 gagttgattc aaacgatgtt gctccgctcg ctgaaacaag cagtgtatgc ggcggataat
    10801 atcgggcatt tcggcttggc attaaccgaa tatgctcatt ttacctcgcc gattcgccgt
    10861 tacccggatt tactgttaca ccgtgcaatt aaatatctga tcgaaaaagg caaaggcaac
    10921 actcgctact ataccgatgg cggtggttat cactacaagt tagatgatat ggacgaattc
    10981 ggcgataaat gttctgccac cgaacgccga gctgatgagg caacccgaga agtggcagat
    11041 tggctgaaat gcgaatttat gcaagatcat cttggcgaag agtttgatgg cgtgatttcg
    11101 agtgttaccg gctttggctt atttgtaaaa ctcaatgagc ttttaattga cggattggtt
    11161 catatttcaa cgctggataa tgattattac cactatgatg cggaccgaca acgtttaatc
    11221 ggtggcagtg ggatcatcta tcgtttaggc gatgcagtta gagtaaaagt aatcaatgtg
    11281 aatttagacg agaaaaaaat tgattttgag ttactcaccg gtaataagaa aaccggcaaa
    11341 accgccaaga aaaaagcaaa agcgacagag gttcctgttt tcaaagagcc taaaacggta
    11401 aaaaaagcca aaaaagtcgc tgagaaaaaa ccggcaaaat ctgcaaaaaa acggacaaaa
    11461 acgaccgcta gtaaatcaag gaaaaaatag caaatggatt acaaaaaaca aattttattc
    11521 agccttttat ttgctttagt aattgcaata ataagctatt ttttgtttac ctatatcccg
    11581 tttgattgta gcgatctgag caaactcaac gattcacaac gtttagagtg ccgaactgcc
    11641 gaagttctgg gcgtttatta ctggctgccg cacactacca cgctgttttt cggctcggtg
    11701 ttattatatt gggcggttta tggcatgatt tcattagtgc aagcagtgct caaacgctaa
    11761 caagcggttt tgtttttgca aaaagttgca aatctcatct gaataatcct tccaagatca
    11821 aaggtctatc aaaaaagata gacctttatt gtgtaaataa actaaaactc cttgatagat
    11881 gtatcacata tctataaaat tccagcaatt aagtgggaaa aagtggtaaa ttgtggaact
    11941 tttccttaaa tttaacctca tttaaaaagg taaatcagta aaatgtttcg tggtgcaagt
    12001 tcaatcagca ttgatagcaa agggcggatc gctattccga cccgttatcg tgccgagcta
    12061 ttggaaaaac atcacgggat cttagtttgc actgtggata tccgccagcc ttgtttgttg
    12121 ctttacccac ttcacgaatg ggaaatggtt gaacagaaat tgttggcact atctaatttt
    12181 gaccctgttc aacgccgtat tcagcgagta atgcaaggct ttgctaccga atgtgaaatg
    12241 gattcggcag ggcgaatact gcttagccct acacttcgcc aacacgcaca attagagcaa
    12301 caaattatgc tggttggaca attaaacaaa tttgaaattt ggcaagataa acaatggcaa
    12361 gcacaaattg ccgaagatct tgcttttggc agttcggcag aaattttaga ctgcgaagca
    12421 ttgaaaaacc tgtctctcta gtaattggaa attgagatga gcgagaaccc aaaacatatt
    12481 acagtattat tgcacgaagc ggttgacggc ttggcaatta agcctaacgg tatctatatt
    12541 gacggtactt tcgggcgtgg cgggcattct cgcttgattt tatccaaact gggcgaaaat
    12601 gggcgattaa tcgcaactga tcgtgaccca cgagcgatag ccgaagctca aacgattagt
    12661 gaccctcgtt ttcaaattga acatacggca ttttctgcca ttcccgaact gtgtgaacga
    12721 atggggttgg tgggcaaaat tgacggtatt ttattggatt taggtgtgtc ttccccgcag
    12781 cttgacgaag ccgaacgagg ctttagtttt atgcgtgacg ggccgcttga tatgcgaatg
    12841 gataccacca aaggcttatc ggcagccgag tggcttgccc aagtttcggc agaagattta
    12901 gcttgggtat tgaaagaatt tggcgaagag cgttttgcca aacggattgc acaagcggtc
    12961 gtttcttaca ataaatctgc aaatgaaaaa atcagccgca ctttgcagct tgcgcaaatt
    13021 attgcggatg cggtgccgtt taaagataag cataaacacc ctgcaacccg ttcgttccaa
    13081 gcaattcgca tttttatcaa tagtgaatta gacgagctgg aaaaagcttt aaattccgca
    13141 ttaacggtgt tagcccctga agggcgtttg tcgattatca gctttcattc tctagaagac
    13201 cgaatggtga aacagtttgt gcgtaagcat agtaaaggaa tggacgtacc ccgtggttta
    13261 ccgattttag aagcagagct aaccaaaaat attccgctga aaaccattgg
//
LOCUS       DANJWC010000054        13250 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.054, whole genome shotgun sequence.
ACCESSION   DANJWC010000054 DANJWC010000000
VERSION     DANJWC010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13250
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.054"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            459..1103
                     /gene="rppH"
                     /locus_tag="PWX02_001146"
     CDS             459..1103
                     /gene="rppH"
                     /locus_tag="PWX02_001146"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HDL1729168.1"
                     /translation="MIDFDGYRPNVGIVICNKHGQVLWAKRFGQNSWQFPQGGINEGE
                     NIEAAMYRELYEEVGLSKKDVRLLWASKYWLKYKLPKRLVRNEGSGPVCIGQKQRWFL
                     LQLIGDESLINLKTTKNPEFDGWRWVSFWYPVRQVVSFKRDVYRKAMKEFAQVLQNNI
                     QKATDDKTGEKPRIERGETSPHTYKKTSQDSAKAYKREARKPYRPWHQHNQKGK"
     gene            1105..1893
                     /locus_tag="PWX02_001147"
     CDS             1105..1893
                     /locus_tag="PWX02_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL1729169.1"
                     /translation="MTILLSCLFLGVFVGFLAGLFGIGGGLIIVPSLVFLLPMAGVPP
                     EYIMSTALGTSFSTIIITAFASAQRHHKLGNVDIQTSKYFIPALMVSVFLSGLVVSRL
                     DPQLMSRIFAVMVLYLAGKMIFSIKRAPKNKPLTVQTTIIGGSIIGALASMAGIAGGA
                     FIVPFLNDRGLEMKRAIGTSSFCGAFLGLSGTISFIVSGWGLEGMPDYSLGYVYLPAL
                     FGITATSFFTSKMGASAANALPVPMLKKAFAVLLVCIAINMFLK"
     gene            1901..2698
                     /gene="lgt"
                     /locus_tag="PWX02_001148"
     CDS             1901..2698
                     /gene="lgt"
                     /locus_tag="PWX02_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDL1729170.1"
                     /translation="MNEQFIQFPQISPIVFEIGPIALRWYGLMYLAGFAFAYWLGMRR
                     AKNSNGVWTTEQVDSLVYTCFWGVVLGGRIGDVFFYSFDRFLQDPLYLFRIWEGGMSF
                     HGGLIGVIVAMIWVSYRQKRNFWQTADFIAPLVPFGLGMGRIGNFINDELWGRVTDVP
                     WAVLFPSGGYLPRHPSQLYEAFLEGVVLLFILNWFIQKPRPAGAVSGLFLIGYGTFRF
                     IVEYFREIDHNVNTTADLITRGQLLSLPMIIGSILIMAWAYKVNKVK"
     gene            2785..3453
                     /locus_tag="PWX02_001149"
     CDS             2785..3453
                     /locus_tag="PWX02_001149"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HDL1729171.1"
                     /translation="MSKKYKVIGFDLDGTLINTLPDLTLVANSMFLEAGLPTVTQEKV
                     LTWIGKGADIFFQNAVAHTGRIFQADELVKMRVSFDKYYATYVCEESTLFPNVKETLE
                     ILKANDYPLVVITNKPTKLVEPVLSAFGIYHLFDETLGGQSLPKIKPHPDPMFFICEK
                     FGITPSELLFVGDSENDVIAAKAAGCDVVGLTYGYNYNVPIEQSNPTFVTSEFKDVLK
                     IVGL"
     gene            3523..3783
                     /locus_tag="PWX02_001150"
     CDS             3523..3783
                     /locus_tag="PWX02_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDL1729172.1"
                     /translation="MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVI
                     NPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPAIGKEIL"
     gene            3780..4112
                     /locus_tag="PWX02_001151"
     CDS             3780..4112
                     /locus_tag="PWX02_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL1729173.1"
                     /translation="MKRGDIYWLDLDPTLGHEQSGFRPVVIVSATAFNLASKLPVVVP
                     ITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRTVDIAQRHGKFIEALPESLLDEIM
                     ARVSVIFE"
     gene            4239..5270
                     /gene="trpS"
                     /locus_tag="PWX02_001152"
     CDS             4239..5270
                     /gene="trpS"
                     /locus_tag="PWX02_001152"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDL1729174.1"
                     /translation="MSKPIVFSGVQPSGELTIGNYLGALRNWVKMQDDYDCVYCIVDQ
                     HAITVRQDPESLRKSTLDVLALYLACGIDPNKSTIFIQSHVPEHAQLAWVLNCYTYFG
                     EMGRMTQFKDKSARYEENVNVGLFTYPVLMAADILLYQANQVPVGDDQKQHLEITRDI
                     ANRFNALYGKKDSEGNLIEPVFAVPEVFIAKSGARVMSLLEPTKKMSKSDDNRNNVIG
                     LLEDPKAVAKKIKRAMTDGDEPPVVRYDVQNKAGVSNLLDILSAITGKTIAELETEFE
                     GKMYGHLKTEVADKVSELLTNLQERYHHYRQDEALLEKIYREGAEKARAQAKATLDKV
                     YELVGFVRY"
     gene            complement(5334..6089)
                     /locus_tag="PWX02_001153"
     CDS             complement(5334..6089)
                     /locus_tag="PWX02_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDL1729175.1"
                     /translation="MKFIKKLSYSVFLATVITACATTESINQEAAQSYAQVKTQARQQ
                     NAIDNSSATAKRIHTVFHKMKPYAERANQTGVPFHWEITVLKSDELNAWAMPGGKMAF
                     YTGLVERLNLSNDEIAVVMGHEMAHALKEHGKSDRTVSAITSIVGAIADVAVTASTGV
                     STEGLLSTGVDLIATKPFSRSQETEADEVGLILMAESGYNPSAAPNVWVKMSKANGDS
                     GLSIFSTHPSNADRQENLARLVPEAMKIYNARK"
     gene            complement(6145..6672)
                     /gene="ppa"
                     /locus_tag="PWX02_001154"
     CDS             complement(6145..6672)
                     /gene="ppa"
                     /locus_tag="PWX02_001154"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HDL1729176.1"
                     /translation="MGLELVPAGKELPDDIYVVIEIPANSDPIKYEVDKETGALFVDR
                     FMATAMFYPANYGYVNNTLSSDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGG
                     DAKVVAVPHTKLSKEYDHIKDVGDLPALLKAQIQHFFESYKALEAGKWVKVEGWGDVN
                     EARQEILESFERAKK"
     gene            6889..6964
                     /locus_tag="PWX02_001155"
     tRNA            6889..6964
                     /locus_tag="PWX02_001155"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6922..6924,aa:Lys,seq:ttt)
     gene            6987..7062
                     /locus_tag="PWX02_001156"
     tRNA            6987..7062
                     /locus_tag="PWX02_001156"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7020..7022,aa:Lys,seq:ttt)
     gene            7155..8189
                     /gene="mltG"
                     /locus_tag="PWX02_001157"
     CDS             7155..8189
                     /gene="mltG"
                     /locus_tag="PWX02_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDL1729177.1"
                     /translation="MLKKILIALGLAGVIACGGLFYGYQKLTSLAEHPITVQPNQLFV
                     LEKGVSSQKLAALLEEQGIVTHDDADLIPYLMRLYPELSKFKAGAYSLAGLTTVKDLL
                     AHLSSGKEVQLNVQFIEGKTFKIWREQLAKADYLQHTLQGKSEKEIADLLGIPHEKIE
                     GWIAPDTYNYTPNSTDLALLKRAYQKQKTALEQAWKNRAENLPLATPYEMLILASIVE
                     KETGIATERPQVASVFINRLRNNMRLQTDPTVIYGMGDRYNGNIRRKDLEEATPYNTY
                     QIDGLPPTPIAMPSEASIKAVANPDNTPYLYFVADGTGGHKFSKTLNEHNKAVAEWIQ
                     IERKRKNEQK"
     gene            8200..8820
                     /locus_tag="PWX02_001158"
     CDS             8200..8820
                     /locus_tag="PWX02_001158"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDL1729178.1"
                     /translation="MRGKFIVIEGLEGAGKTTAHQVILKELEQAGINDVVMTREPGGT
                     PLAEKLRHLIKHETEEAINDKAELLMLYAARVQLVENVIKPALAEGKWVLGDRHDMSS
                     QAYQGGGRKLDRHLLETLKESVLGTFEPDLTIYLDLDPVIGLERARGRGELDRIEQQS
                     LDFFYRTRERYLELTQHNDKAVIINAEQPIEQVTADIQQVIRNWLK"
     gene            8820..9797
                     /locus_tag="PWX02_001159"
     CDS             8820..9797
                     /locus_tag="PWX02_001159"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDL1729179.1"
                     /translation="MYPWLESTYQQLTDSFLQGRGHHALLFKTDVGLGTDTLIYKVAE
                     WLLCSQKLRHQPCLECKSCLLMQSNNHPDFHILEPIEDKDIGIDQVRELIYKLQNFAQ
                     QGGNTVVYIRGVDRLTEASSNALLKTLEEPRENVYFLLEAPLQATVLATIQSRSQTWV
                     LYAPQASEVMAWLQTQFPAISTDDLEIALRLCHSRPLICKNFIENDRLQQRKTFLQTF
                     WRFYKSHDVFLLFQAFAQEKELILQQLEWLESFFSDALKAKMAISHGWLNPDLQNGIL
                     PFSQQLTTHGLLKGHQILQQTQRDLTEINAVNPELMLLDCLTKLVLVFE"
     gene            9928..10170
                     /locus_tag="PWX02_001160"
     CDS             9928..10170
                     /locus_tag="PWX02_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDL1729180.1"
                     /translation="MARTTSVTLGEPLNDFVNEMVASGRYGSTSEVVRTALRMLEEKE
                     QYLAQLRQAIDDGDASGYATETIDEMMLRVKAELNV"
     gene            10163..10447
                     /locus_tag="PWX02_001161"
     CDS             10163..10447
                     /locus_tag="PWX02_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL1729181.1"
                     /translation="MYKLSAQALRDQEKLFRYSISQFGVQQTESYFADLKHILNLLAD
                     FPTMGTSAEHIRGGLRMHTHQSHTIYYRIKADHIFIVRILHNQMNHKRYI"
     gene            10452..11240
                     /locus_tag="PWX02_001162"
     CDS             10452..11240
                     /locus_tag="PWX02_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchF/TatD family DNA exonuclease"
                     /protein_id="HDL1729182.1"
                     /translation="MKDLFIIDSHCHLDSLDYETRHKNVDEVIENAKARGVHHFISVC
                     TTVGRFEAMKNLTAHRNDVSLSCGVHPLNVEDEPFDYDKLFQFAQDPKVVAIGETGLD
                     YHYTPETKALQQSLFVQQIEIANKLNKPLIIHSRSARQDTMDMLEQNNAEKCGGVLHC
                     FTEDWAMAKRALDIGFYISISGIITFRNAEELREVVRKVPLDRLLVETDSPYLAPIPY
                     RGKPNQPAYVRETCEYVATLKGVSTEELARITTENVQHLFNIKL"
     gene            11250..11771
                     /locus_tag="PWX02_001163"
     CDS             11250..11771
                     /locus_tag="PWX02_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1523 family protein"
                     /protein_id="HDL1729183.1"
                     /translation="MRAILKYFLILVSLSFFIVLGGAVNYAMPSYEDTVVTGMEVRRM
                     DKDGIISKANPADGEVRDVYFLFTEDPETKKVMVYRNEDTGWGLPPYFKFGSADIQAK
                     AQAYANEKQRVQIKYYGWRINWLNEFRNIVSIKPLEEAETVSKPIMSYVLYAVLAFLF
                     FLSVQFIRGIFKD"
     gene            11846..12547
                     /gene="lpxH"
                     /locus_tag="PWX02_001164"
     CDS             11846..12547
                     /gene="lpxH"
                     /locus_tag="PWX02_001164"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HDL1729184.1"
                     /translation="MTYYFIADLHLNENQPEITHLFLQFMQEKALLAEAVYILGDLFD
                     FWIGDDEQSELISTVKQAIKTLTTSGTKCYFICGNRDFLIGKRFSEQTGMVILPDYHV
                     IDLFGTSTLLCHGDTLCTDDVKYQQFRKKVHQKWRQAIFLMFPLSWRIRIAEKIRSKS
                     KQEKRDKSAEIMDVNAEVTAQIVAKYNTTQLIHGHTHRQAIHQHNGFTRIVLGDWKEN
                     YASILSVTEQGAEFI"
     gene            12544..>13250
                     /locus_tag="PWX02_001165"
     CDS             12544..>13250
                     /locus_tag="PWX02_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL1729185.1"
                     /translation="MINVIIPCYNAENTLVRAVQSVLNQSGLNLLWLIDDASTDNTLA
                     LAKHLQAQVPDKIRVEQMPQNGGVAKARNWGALQSETDLIAFLDADDAYENGALQVAE
                     KIFEFKPETGLVRLALKPVNLDQRYSSHPNFELAWQYMQMTGAGNTVFRRSFFLACGG
                     FPQHQLFQELGGEDGALGIATTQISSVATAFNDVGVLHYCREGMHAERLLNAILFNEK
                     DPNVTEEKVKQANLITE"
BASE COUNT         3943 a         2818 c         2753 g         3736 t
ORIGIN      
        1 aaaacaattg attattcaca gattgaaaag atgacactaa ctatttgatt taattataat
       61 ttatggaaat agcaaaaggc tgatttgagt tattcacgcc taatttgaaa aactatcaca
      121 tctttatcca caaatggtgg ggataactct gctttattta cgaagcaaaa attgccctgc
      181 ttattcattt taaaatttaa acttattttg acgagaaaaa acgcaacaac ttgatcttta
      241 tccagatcat tgcttaagaa aaagatcgca tttttagatc tgattaattg ctctgtttta
      301 caacgttccg cctgtttatc ctcaacttat accacttata ttcaatagtt atccacaaag
      361 ttattcacaa gcggttatat tttatggttt ttttgcaaat caacccaaag tatgaaacaa
      421 ttctcccatt ctagaatttt tatcaattag gtggtcgagt gatcgatttc gatggctacc
      481 gcccaaatgt tggaatcgtt atctgtaata agcacgggca agttctgtgg gcaaaacggt
      541 ttggtcaaaa ctcttggcaa tttccgcaag gtggaattaa tgagggggaa aacattgaag
      601 cagcaatgta ccgtgagctt tatgaagagg tcggcttaag taaaaaagat gtacgattgc
      661 tttgggcgtc taaatattgg ctaaaatata aactgccaaa acgcttggta cgtaatgaag
      721 gctctggtcc tgtctgtatc gggcaaaaac agcgttggtt tctactgcaa ttaatcggtg
      781 atgaaagcct gattaatttg aaaaccacga aaaatccgga gtttgacggc tggcgttggg
      841 tcagcttttg gtatccagtc cgccaagtgg tctcttttaa acgagatgtt tatcgcaaag
      901 cgatgaaaga gtttgcacag gttttgcaaa acaatattca aaaagcgact gacgataaaa
      961 ccggtgagaa accacgcatt gagcgtggcg agacttcgcc ccatacttat aaaaaaacct
     1021 ctcaggattc tgccaaagcc tataaacgag aagctcgtaa gccttatcgt ccttggcatc
     1081 aacataatca aaaaggaaaa taagatgact attttattaa gctgcctttt tttaggggtg
     1141 tttgtcggct ttttagccgg cttgttcggc attggtggtg ggttaatcat cgtgccgagt
     1201 ttggtcttcc tactgcccat ggcaggtgta ccgccggaat atataatgtc caccgcactc
     1261 ggcacatcat tttcaaccat tattattacc gcctttgcat cggcacaacg ccatcataag
     1321 ttaggcaatg tcgatatcca aaccagtaaa tattttattc cagcgttgat ggtttcggtt
     1381 tttctatcgg gcttggtggt aagccgcctc gatccgcaac tgatgtctag aatttttgcg
     1441 gtaatggtac tttaccttgc aggtaaaatg attttttcaa taaaaagagc accgaaaaat
     1501 aaaccactta ccgttcaaac gacgataatc ggaggttcaa tcatcggtgc gttagccagt
     1561 atggcaggga tcgcaggtgg agcctttatt gtaccatttt taaacgatcg aggcttagaa
     1621 atgaaacgtg ccatcggcac ttcctcattt tgcggagcat ttttaggttt atctggcaca
     1681 atcagcttta ttgtaagtgg ctggggctta gaaggaatgc ctgattattc tctcggttat
     1741 gtttatttac cggctctgtt tggtattacg gcaacttcat ttttcacctc aaaaatgggg
     1801 gcaagtgccg ccaacgctct acctgtgccg atgctgaaaa aagcctttgc ggtgcttttg
     1861 gtctgtatcg ccatcaatat gtttttaaaa taaggacact atgaacgaac aatttatcca
     1921 atttccacaa attagcccga ttgtgtttga aatcggtccg attgccctgc gttggtatgg
     1981 gctaatgtat ttagccggct ttgcctttgc ctactggctg ggaatgagac gggcgaaaaa
     2041 ttccaatgga gtttggacaa ccgagcaagt ggatagctta gtctatactt gcttctgggg
     2101 cgtagtatta ggtgggcgta tcggcgatgt gttcttttat agcttcgacc gtttcttaca
     2161 agatccgctc tacctctttc gcatttggga aggcggaatg tcctttcacg gcggtttaat
     2221 cggcgtcatt gtagcaatga tttgggtttc ctaccgccaa aaacgcaatt tctggcagac
     2281 agcagatttt atcgcaccgc tagtaccgtt cgggctaggt atggggcgta tcggcaattt
     2341 cattaacgat gaactttggg ggcgtgtaac cgatgtgcct tgggcggtgt tatttccaag
     2401 cggtggctat cttccccgcc acccgtcaca gctttatgaa gcctttttag agggtgtggt
     2461 gttactcttt atcctcaact ggtttattca aaagcctcgc ccggcaggtg cggtttcagg
     2521 gttattttta atcggctacg gcacattccg ctttattgtg gaatacttcc gagaaatcga
     2581 ccataacgtc aataccaccg cagatttaat cactcgtggg caactactct ctttaccgat
     2641 gattatcggc agcatactga ttatggcgtg ggcgtataaa gtaaacaaag tgaaataaac
     2701 gtaggggcaa gtcaatattt gccctcatta caaaaattgc aaaaaatttg cagaaaacga
     2761 ccgcttgtgt ttgaaggata tactatgagt aaaaaataca aagtcatcgg tttcgactta
     2821 gacggaacgc taatcaacac cctccccgat ctcactcttg tcgccaattc aatgtttcta
     2881 gaagccggtt tgccaacggt tacacaagaa aaagtgctga cttggatcgg taaaggggca
     2941 gatattttct tccaaaatgc ggttgctcac accggcagaa ttttccaagc cgatgagttg
     3001 gtgaaaatgc gagtgagctt tgataaatac tacgccacct atgtgtgcga ggaaagcacc
     3061 cttttcccaa atgtgaaaga aaccttagaa atcctcaaag caaacgacta cccgctggtg
     3121 gtgattacca acaaaccgac caaattagtc gagccggtgc tttccgcttt cggcatttat
     3181 catttattcg atgaaaccct tggtgggcaa tcgctcccca aaatcaaacc gcaccctgac
     3241 ccgatgtttt ttatctgcga aaaattcggc attaccccat cagaattact ttttgtcggc
     3301 gactcagaaa atgacgtgat tgccgccaaa gccgccggtt gtgatgtggt tggactaact
     3361 tacggctata actacaatgt gccgattgaa caatctaacc cgacttttgt gacatctgaa
     3421 tttaaagatg tgttgaaaat tgtggggtta taaagaaaac atttgttttc ttttgatgtt
     3481 tgctttagac tttccgaacc taaagattta aaggagtaaa gtatgccaac aatcgcatta
     3541 cgtcaggtcg gtggctcggt tgcaatggtg attccgcctg cttttttaga ggcactcaag
     3601 ctctccgtcg gtagcaaagt ggatttgtct ttaaacgaca accaactcgt gattaatcct
     3661 gcccgcccaa aagtgaaata cagcttagag gaattattaa gacaatccga ttttgaggca
     3721 gcaaggaatg aagaaagcca atcgtggtta tccgagccgg caataggaaa agaaatttta
     3781 tgaaacgtgg cgatatttac tggctcgatc ttgatccaac cttaggacac gaacaatcag
     3841 gcttccgccc cgttgtaatt gtttccgcaa ctgcatttaa tctagccagt aaactgcctg
     3901 tggttgtgcc gattactaac ggtggagctt ttgctgaaag actgggcttt gccgtttcat
     3961 taaaagatgc cggcttaaaa acaacaggga ttattcgatg cgatcaacct cgcacagtcg
     4021 atattgcaca acgccatgga aaatttatcg aagccttacc cgaaagccta cttgatgaaa
     4081 ttatggcaag ggtttcagtg atttttgagt aagatacaag cggtctgatt tgctctattt
     4141 tttgcaaaat catcaataaa tctgaccgct tgtactttta tcccttccca ataaccgtta
     4201 agataaccta tatttttcaa atcaaacgag aagataaaat gagcaaacct attgtattta
     4261 gtggcgtgca gccctcaggt gaattgacta tcggtaacta cttaggggcg ttacgcaact
     4321 gggtgaaaat gcaagatgat tatgactgtg tatattgcat tgtcgatcaa cacgccatta
     4381 ccgttcgtca agatccggag tcgctacgca aatcaacttt agatgtgtta gccctgtatt
     4441 tagcttgtgg cattgatcca aacaaaagca ctatttttat tcaatcccac gtgccggaac
     4501 acgcccaatt agcttgggtg ctgaactgtt acacctattt cggtgaaatg gggcgtatga
     4561 cgcaatttaa agataaatcg gcacgctatg aagagaatgt caatgtgggc ttatttactt
     4621 atccggtatt aatggcggca gatattctgc tttaccaagc caaccaagtg ccggtaggcg
     4681 atgaccaaaa acaacatttg gaaatcaccc gtgacattgc caaccgtttc aatgccttat
     4741 acggcaaaaa agattccgaa ggcaacctga tcgagcctgt ttttgcagtg ccggaagtct
     4801 ttattgcgaa atccggggca agagtgatgt cgctacttga gccgaccaaa aaaatgtcaa
     4861 aatcggacga taatcgtaac aatgtgattg gactattgga agatccgaaa gcggtagcga
     4921 agaaaatcaa acgggcaatg accgatggcg atgaaccgcc agtagtgcgt tatgacgtac
     4981 agaataaagc cggtgtctcg aatttattgg atattctctc cgccattacc ggcaaaacca
     5041 ttgccgaatt agaaaccgaa tttgaaggca aaatgtatgg tcatttgaaa acagaagtag
     5101 cagataaggt ttccgaacta ttaaccaatc tgcaagaacg ttatcaccac taccgccaag
     5161 atgaagcatt attggaaaaa atctaccgtg aaggggcaga aaaagctcga gctcaagcca
     5221 aagcaacgtt agataaggta tatgaattag tcggttttgt gcgttactaa cacttaatga
     5281 aaaatgctct tgaggattca agagcatttt tgcaaaaaag aaccgattgt caattattta
     5341 cgggcgttat aaatcttcat tgcttccggc acaaggcggg ctaagttttc ttgacggtca
     5401 gcatttgatg ggtgagttga gaaaatggac aagcctgaat cgccattggc tttactcatt
     5461 tttacccaaa cattcggcgc tgctgatggg ttgtagcctg attctgccat taaaattaac
     5521 ccaacttcgt ccgcctcagt ctcttggctg cgagagaatg gcttggtggc aatcaaatcc
     5581 acgccagtgc tgagtaaacc ttcggtgctt acgcctgttg aggcagtcac agccacatct
     5641 gcaatagcac ctacaatact ggtaatagcg ctaaccgtac ggtctgattt accgtgttct
     5701 ttcagtgcgt gagccatctc gtgtcccatt accactgcaa tttcatcgtt cgataaatta
     5761 agtctttcca ctaaccccgt gtaaaacgcc attttaccgc ccggcatcgc ccacgcattg
     5821 agttcatccg atttcaatac ggtaatttcc caatgaaaag gtacaccagt ctggttggct
     5881 ctttccgcat aaggcttcat tttatgaaaa acagtgtgaa tccgcttagc ggtggcagac
     5941 gaattgtcaa tcgcattttg ctgacgagct tgggttttca cttgagcata actttgagcc
     6001 gcttcttggt taatgctttc tgttgtggca caagcggtaa tcactgttgc taaaaaaact
     6061 gaataactta attttttgat gaatttcatt ttatatccca caaaaaatgc caaacaaatt
     6121 tgcttggcat tcaaaaataa ataattattt cttagcacgc tcgaatgatt ctaagatttc
     6181 ttggcgagct tcgtttacat cgccccaacc ttctactttc acccatttgc ccgcttctaa
     6241 tgctttatag ctttcgaaga agtgctggat ttgtgctttt aataacgccg gtaagtcacc
     6301 tacatctttg atgtggtcat actctttact taatttggtg tgtggaacag caaccacttt
     6361 tgcatcaccg ccggcttcat cggtcatttt taacacaccg acaggacggc aacgaatcac
     6421 cgaacccggt tgtaacggat atggtgttgg cactaataca tcaaccgggt caccgtctga
     6481 tgataaggtg ttattcacat aaccatagtt tgccggatag aacattgcgg ttgccataaa
     6541 gcgatccaca aataatgccc cggtttcttt gtccacttca tatttgattg gatcagagtt
     6601 tgccggaatt tcaattacaa cataaatatc gtccggtaat tctttaccgg caggaactaa
     6661 ttctaagccc attttggttt ccttctataa taggtgaaat aaacggcgtg attatagcct
     6721 attttcagaa aaagataaaa gcccgtttct cttttcattt tccgccaaca aatccggcta
     6781 attttttgct aaattgctta caaaccgcac actcaaccat ttttcgataa aaaaaacttg
     6841 ccaaagtttt aaaaatccgt aagatatgcc ctcgttacgc aagattatgg gtcattagct
     6901 cagccggtag agcagcggac ttttaatccg ttggtcgaag gttcgaatcc ttcatgaccc
     6961 accattaatc ttcaaaacac ctctaagggt cattagctca gtcggtagag cagcggactt
     7021 ttaatccgtt ggtcgaaggt tcgaatcctt catgacccac catttactca aatcccattt
     7081 gcaaattttt ctgaaaaaac aaccgcttta tagtagaatt cagtcaattt ttatcacttt
     7141 tattttattc atcaatgcta aaaaaaatcc ttattgcatt aggcttagcc ggcgttattg
     7201 cttgcggtgg actgttttac ggctaccaaa aactcacctc tttagccgaa cacccgatca
     7261 cggttcagcc gaatcaactt ttcgtattgg aaaaaggcgt ttccagccaa aaattagcgg
     7321 ctctgttaga agagcaaggc attgtcaccc acgatgacgc cgatcttatc ccttatttaa
     7381 tgcgattata ccctgagtta agcaaattca aggctggtgc ttattcactc gcaggcttaa
     7441 cgacagtaaa agatttgctc gctcatttaa gctccggcaa agaagtgcaa cttaacgtgc
     7501 aatttattga gggcaaaact tttaaaattt ggcgggaaca actagcgaaa gccgattatt
     7561 tacagcacac gctacaaggc aaatcggaaa aagaaatcgc tgacttactc ggcattccac
     7621 acgaaaaaat cgaaggttgg atagccccgg atacttacaa ctacacgccc aattccaccg
     7681 atttagcctt gcttaaacgg gcttatcaaa agcagaaaac ggcgttggaa caggcttgga
     7741 aaaatagagc ggaaaatcta ccgcttgcaa caccatacga aatgctgatt cttgcctcga
     7801 ttgtggaaaa agagaccggc attgcgaccg aacgcccaca agtggcatcg gtctttatta
     7861 accgtttacg caacaatatg cgactgcaaa ccgatccaac cgtcatttac ggaatgggcg
     7921 atcgttataa cggcaatatc cgccgcaaag atttagaaga ggcaacgcca tacaatactt
     7981 accagattga cggcttaccg ccaacgccga ttgccatgcc gagtgaagct tcgattaaag
     8041 cggttgccaa cccggataat acaccctacc tctattttgt ggcagacggc acgggcggtc
     8101 ataaattcag caaaacactg aatgagcata ataaagcggt ggcagaatgg attcaaattg
     8161 aacgcaaacg caaaaatgaa caaaaatagt gagaacaata tgcgtggaaa atttattgta
     8221 attgaaggct tagagggcgc aggcaaaacg actgctcacc aagtgattct caaagagttg
     8281 gaacaagccg gcattaatga cgtagtaatg acccgagaac ccggcggcac cccccttgcc
     8341 gaaaagttac gccatttaat taaacacgaa accgaagaag ccattaacga taaggctgag
     8401 ctgctaatgc tctacgctgc tcgtgttcaa ttagtcgaaa atgtgatcaa acctgccctt
     8461 gccgaaggca aatgggttct gggcgaccgc cacgatatgt cttcccaagc ctatcaaggc
     8521 ggtggacgta agttggatcg tcacttactc gaaaccttaa aagaatcggt actcggcaca
     8581 ttcgagccag atttaacgat ttatctcgat ctcgatccgg tgattggttt agaaagagcc
     8641 agaggcagag gcgaattaga ccgcatcgaa cagcaaagcc ttgacttctt ctaccgcaca
     8701 cgagagcgtt atttagaatt aacccaacac aacgataaag ccgtgatcat caatgccgaa
     8761 caacccatcg aacaggtgac tgcggatatt caacaagtaa ttagaaactg gctgaaataa
     8821 tgtacccttg gctcgaaagc acttatcaac aacttaccga ttcattttta caggggcgag
     8881 gtcatcacgc cttactgttc aaaacagatg ttggtcttgg tacagacacc ttaatctaca
     8941 aggtggctga atggttgctt tgcagccaaa aactccgcca tcaaccttgt cttgaatgca
     9001 aaagttgcct gcttatgcaa agcaacaacc accccgattt ccacattctt gagccgattg
     9061 aagacaaaga tattggtatc gaccaagtgc gtgagttgat ttataaactc cagaatttcg
     9121 cccaacaagg gggaaatacg gtcgtttata ttcgaggagt tgatcgttta accgaggcaa
     9181 gcagtaatgc cctgctcaaa accttggaag agccacgtga aaacgtctat ttcttgctcg
     9241 aagcaccgct tcaagcaaca gttttagcta ccattcaaag ccgcagccaa acttgggtac
     9301 tttacgcccc acaagcctca gaagtaatgg cttggctaca aacacaattt ccggcaatct
     9361 caaccgacga cttagagatt gcactccgcc tttgccactc acgcccacta atttgcaaaa
     9421 attttatcga aaatgaccgc ttgcaacagc gaaaaacctt tttgcaaacc ttctggcgtt
     9481 tctacaaaag ccacgatgtc ttcttgttat tccaagcctt cgcccaagaa aaagagctga
     9541 ttttacagca actcgaatgg ctggaaagtt tctttagcga tgccctaaaa gctaaaatgg
     9601 caatcagcca cggttggcta aacccggatc tccaaaacgg cattctccct tttagccaac
     9661 aactaacgac ccacggctta cttaaagggc atcaaattct ccaacaaacc cagcgggatt
     9721 taaccgaaat taacgccgtc aatccggaat tgatgttgct agattgcctg acgaagttgg
     9781 ttttggtgtt tgagtaaaag ccctctctca tagaaggtag ctttcaatta gccgtaaaaa
     9841 gaggcttcta gccctataat atagtttata acttgcaaaa ttatctatct ttgttattgt
     9901 atgaataatt cacacaagga gaatattatg gcaagaacaa ccagcgtgac tttaggcgaa
     9961 cctttaaatg attttgtaaa tgaaatggtg gcaagtgggc gttatggctc taccagtgaa
    10021 gtagtgagaa ccgctttacg aatgcttgag gaaaaagagc aatacttagc tcagttgcga
    10081 caagccattg atgatggtga tgctagtggt tatgcaaccg aaacgattga tgaaatgatg
    10141 ttgagagtaa aggcggaact taatgtataa actttctgct caagcactcc gagatcaaga
    10201 gaaacttttt cgttacagta tctctcaatt tggtgttcaa caaacagaat cttattttgc
    10261 agatctaaag catattttga atttattagc tgattttcca acaatgggaa cttctgcgga
    10321 acatattcgt ggtggcttgc gaatgcacac acatcaatct catacaattt attatcgaat
    10381 aaaagcagat catatattta ttgttcgcat cttacataac caaatgaatc ataaacgcta
    10441 tatttagaaa aatgaaagac ttatttatca tagactcaca ttgtcatcta gattctctag
    10501 actacgaaac tcgtcacaaa aacgtggacg aagtaattga aaatgccaaa gcccgagggg
    10561 tgcatcattt tatctcggtt tgcactacag ttgggcgttt tgaagcaatg aaaaacttaa
    10621 ccgcccatcg taatgacgtt tcactttctt gtggcgttca cccgttgaat gtggaagatg
    10681 aaccttttga ttacgataaa ttatttcagt tcgctcaaga tccgaaagtg gttgccattg
    10741 gggaaactgg tttggattat cactacacac cggaaaccaa agcgttgcaa caatcgcttt
    10801 tcgttcaaca aattgaaatt gcaaacaaac tcaataagcc actcattatc cattctcgtt
    10861 cggcacgcca agatacgatg gatatgttgg aacaaaataa tgccgaaaaa tgcggtgggg
    10921 tgttgcattg ctttaccgaa gattgggcaa tggcaaaacg ggctttagac atcggctttt
    10981 atatctcaat ttcgggcatt attactttcc gcaatgcaga agaactccga gaagtcgtgc
    11041 gaaaagtgcc gttagatcgc ttattggttg aaacagattc cccttatctt gccccgattc
    11101 cttatcgtgg taagccgaac cagccggcgt atgtgcggga aacttgcgaa tatgtggcga
    11161 cattaaaagg cgtttcaact gaagaattag ctcgcattac aaccgaaaat gtgcaacact
    11221 tattcaacat taaattataa ggaactaata tgagagcaat acttaagtat tttctgattc
    11281 ttgtctcact atcatttttt attgtattag gcggtgcagt taattatgca atgccaagtt
    11341 atgaagatac cgtagtaaca ggtatggaag tacgccgaat ggataaagat ggtattatca
    11401 gcaaagcgaa tcctgctgat ggcgaggtgc gcgatgttta tttcttattc acggaagatc
    11461 ctgaaaccaa aaaggtaatg gtttatcgta atgaagatac cggctggggg ttgcctcctt
    11521 atttcaaatt cggatctgcc gacattcaag ccaaagctca agcctacgct aacgaaaaac
    11581 aacgtgtgca aattaaatat tacggctggc gaatcaattg gctaaatgaa tttagaaata
    11641 ttgtttcaat caagccatta gaagaagcag aaacagtcag caaaccaatt atgagctatg
    11701 tactttatgc cgtattggcg ttcttgttct tcctctcagt acaatttatt cgtggcattt
    11761 ttaaagatta atgccgatta acctttgaca aacggttcga ttttgtaaaa aattgaaccg
    11821 ttttcttttt tatcaacaat atcaaatgac gtactatttt attgccgatc tccacttaaa
    11881 tgaaaatcag cctgaaatta cccacctctt tttgcaattt atgcaagaaa aagcactact
    11941 tgcagaagcg gtttatatct taggtgatct gtttgatttc tggattggcg atgatgagca
    12001 gtctgaactc atttcaaccg ttaaacaggc aatcaaaacc ctcactacaa gcggtacaaa
    12061 atgctatttt atttgcggaa atcgtgattt tttaattggc aaacgattca gcgaacaaac
    12121 gggtatggtt attttgcctg attatcacgt tatagattta ttcggcacat ctaccttgct
    12181 ttgccacggc gatacgcttt gtactgatga tgtgaaatat caacaatttc gtaaaaaagt
    12241 ccaccaaaaa tggcgacaag ctatcttttt gatgtttccg ctttcttggc gaattcgtat
    12301 tgcggagaaa attcgatcca aaagcaaaca agaaaagcgt gataaatccg ctgaaattat
    12361 ggacgtaaac gctgaagtta ccgctcaaat tgtcgcaaaa tacaatacaa ctcaacttat
    12421 acacgggcat acgcatcgac aagcaatcca tcagcataat ggatttaccc gcattgtatt
    12481 aggcgactgg aaagaaaatt acgcctctat tttaagcgta accgagcaag gagctgaatt
    12541 tatatgatta atgtgattat tccttgctat aacgccgaaa acacccttgt tcgtgcagtg
    12601 caaagtgtat taaatcagtc tggattaaat ctattgtggc ttattgatga tgcttcaacg
    12661 gataatacgc ttgccttagc taaacattta caagcccaag tgcctgataa aattagagtg
    12721 gaacaaatgc ctcaaaatgg cggtgttgcc aaagcacgca actggggagc attacaaagt
    12781 gaaacagatt taattgcctt tttagatgct gatgatgctt atgaaaacgg ggcattgcaa
    12841 gttgctgaaa agatttttga gtttaaaccg gaaaccggct tggttcggtt agcattaaaa
    12901 ccggtaaact tagatcaacg ttacagctct caccctaatt ttgagttagc ttggcaatat
    12961 atgcaaatga ctggtgcagg caacactgta tttcggcgta gtttcttcct cgcttgtggc
    13021 ggctttccac aacatcagct cttccaagaa ttaggtggag aagatggtgc tttaggcata
    13081 gccactacac aaattagctc tgtagctact gcatttaatg atgtcggtgt tttacattac
    13141 tgccgagagg gtatgcacgc agaaagatta ttaaatgcga ttttattcaa cgaaaaagat
    13201 cccaatgtta ctgaagaaaa agttaaacaa gccaacctaa ttaccgaaaa
//
LOCUS       DANJWC010000055        13197 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.055, whole genome shotgun sequence.
ACCESSION   DANJWC010000055 DANJWC010000000
VERSION     DANJWC010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13197
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.055"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(82..867)
                     /gene="znuB"
                     /locus_tag="PWX02_001166"
     CDS             complement(82..867)
                     /gene="znuB"
                     /locus_tag="PWX02_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HDL1729186.1"
                     /translation="MFDILFPAWLAGVLLAFITAPLGAFVVWRKMAYFGDTLSHSALL
                     GVALGIFLELDPYLAVIGMTILLALLLVWLEQKTNYSVDTVLGIIAHTSLSLGVITIS
                     LLDNVRVDLMSYLFGDLLAINFSDVVFIGIGATLICLILMLFWKRLLSITISPELAQI
                     EGLNVARLRLLLMILTALTIALSMKFVGALIITSLLIIPSATARRFAKTPEKMVLIAI
                     LFSIVAVTGGLLLSGWKDTPAGPSVVICAGLIFLLSLFKKEYH"
     gene            complement(954..2291)
                     /locus_tag="PWX02_001167"
     CDS             complement(954..2291)
                     /locus_tag="PWX02_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDL1729187.1"
                     /translation="MSSLSFDFSEDFRPLAAKMRPRNLAEYVGQSHLIGEGKPLRRAI
                     EAGHSHSMIFWGPPGTGKTTLAEIIAHHLDAEVERISAVTSGVKEIREVIEQAKLNRQ
                     AGRRTLLFVDEVHRFNKSQQDAFLPHIEDGTIIFIGATTENPSFELNNALLSRARIYI
                     LKPLQAVEILKILQMAIADTERGLGNETLVLEDDVLALLADYVNGDARFALNCLELMV
                     DMAQDSAKGKVLNKSLLTEVLGERQARFDKGGDRYYDLISALHKSIRGSSADGALYWY
                     ARILTAGGDPLYVARRLLAIASEDIGNADPRAMQIAIVAWDCFTRVGAYEGERAIAQA
                     IIYLAVAPKSNAVYQAFNEAKRLAKEAKDYDVPAHLRNAPTQLMKSLGYGEEYRYAHN
                     EPNAYAAGENYFPPELKDTEFYFPSERGMEKQIKEKMAWLKAQDQASPIQRYK"
     gene            complement(2301..2789)
                     /gene="mreD"
                     /locus_tag="PWX02_001168"
     CDS             complement(2301..2789)
                     /gene="mreD"
                     /locus_tag="PWX02_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDL1729188.1"
                     /translation="MRTNPIFQILVIISIFVVAFVLEIMPWPVGFQGLRPTWVVLVLI
                     YWALALPDKMSVGTAFIAGIVWDLILGSILGIHALVLSVAIYFVAKYHLILRNLSLWL
                     QSLLVMAYIVLIRFFIFVIEYILHSAEFNSQELLGAVISGVLWSWIFLLMRHIRRQLR
                     LR"
     gene            complement(2789..3844)
                     /gene="mreC"
                     /locus_tag="PWX02_001169"
     CDS             complement(2789..3844)
                     /gene="mreC"
                     /locus_tag="PWX02_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDL1729189.1"
                     /translation="MKPIFAKAPSLGMRLIIAVIFSIMLIISDGRSSLMIQARNLLET
                     AVSGLYYFANTPRSILDGVSGNFIDNNKLQLENNLLKEQLREKSADLLLLDQLKVENQ
                     RLRLLLSSPLRQDEYKKIAEVLAAEMDAYRQQVVINQGRLDGAFVGQPIIDERGVVGQ
                     VISVGEKSSRVLLLSDITHAIPVQVLRNDVRGIASGTGVNNELVIDNLPRSIDVVKGD
                     VLVTSGLGGRFPEGYPVAIVETVENDTKSQFARIVARPLASFDRLRYLLLLWPTAEEK
                     NYSQSLSPAQVREVVEERRNSVNPFERLKQMSNKKTEVQLEAKEEQIENDEINENPAA
                     QPEAENEQNYHTEEGAE"
     gene            complement(3946..4998)
                     /locus_tag="PWX02_001170"
     CDS             complement(3946..4998)
                     /locus_tag="PWX02_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDL1729190.1"
                     /translation="MFKKFLGLFSNDLSIDLGTANTLVYVKGQGIVLDEPSVVAVRQD
                     RMGSLKSIAAVGSHAKLMLGRTPKSIMAIRPMKDGVIADFFVTEKMLQHFIKQVHSNN
                     FMRPSPRVLVCVPAGATQVERRAIKESAIGAGAREVYLIEEPMAAAIGAGLPVHEAMG
                     SMVIDIGGGTTEVAVLSLNGIVYSTSVRIGGDKLDEAIIAYVRRHFGSAIGEATAERI
                     KQEIASAKIESDDDVKTMEVHGHNLAEGAPRTFELNSRHVLEAIEQPLNGIVEAVKSV
                     LEQCPPELAADIFERGMVLTGGGALLHNLDRLLSDSTGVQVVVAEDPLTCVARGGGKA
                     LEMIDMHGGDVFSIDD"
     gene            complement(5113..5742)
                     /locus_tag="PWX02_001171"
     CDS             complement(5113..5742)
                     /locus_tag="PWX02_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HDL1729191.1"
                     /translation="MDIFIQGFIVCFGLIVSIGAQNAFLLKQGILKQHIFWIALLCFL
                     GDVFLMTIGVLGLGTLIAELPVVSFIISLLGAIFLLSYGSRSFISVFKSGEYLVASGE
                     NATSLKKALLITFAITFLNPHVYIDTVVIVGSIGGKLDFNGKIYFLAGALLCSFIWFF
                     GIGYGASLLSPYFAKRRTWQILDAVTGLIMYFIAFSLLIYTYQLALQIF"
     gene            5846..6493
                     /gene="udk"
                     /locus_tag="PWX02_001172"
     CDS             5846..6493
                     /gene="udk"
                     /locus_tag="PWX02_001172"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDL1729192.1"
                     /translation="MTDTSQTSSCVVIAIAGASASGKSLIASTIYKELKNELGTDNIG
                     IISEDAYYRDQSHLTMEERIKTNYDHPNSMDHHLLVEHLRQLKQGNAIEIPEYDYTEH
                     NRKTTTKHFEPKKIIILEGILLLTDENIRNEINVSIFVDAPLDICFIRRLQRDMVERG
                     RSMESVISQYRKTVRPMFLQFIEPSKQYADIIVPKGGKNRIAIDILKAQIKQLLK"
     gene            6508..7089
                     /gene="dcd"
                     /locus_tag="PWX02_001173"
     CDS             6508..7089
                     /gene="dcd"
                     /locus_tag="PWX02_001173"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HDL1729193.1"
                     /translation="MRLCDTDIKRYLDEGIIAITPRPSDDKISGATADVRLGNSFRVF
                     REHKTPYIDLSGPREEVAAQLNKVMSDEIIIGDDEAFFLHPGDLALATTLESVSLPAN
                     IVGWLDGRSSLARLGLMVHVTAHRIDPGWEGKIVLEFFNAGKLPLALRPNMAIGALSF
                     EILSGDAAKPYNARKDAKYKNQQSAISSRINQD"
     gene            7167..7322
                     /locus_tag="PWX02_001174"
     CDS             7167..7322
                     /locus_tag="PWX02_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009041538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HDL1729194.1"
                     /translation="MLHYAVVFFVIAIIAALLGFGGIAGSATEIAKILFFVFLILTVI
                     SFFFKKK"
     gene            complement(7451..8053)
                     /locus_tag="PWX02_001175"
     CDS             complement(7451..8053)
                     /locus_tag="PWX02_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDL1729195.1"
                     /translation="METLIEFFSSYGYWAVFLVLLACGFGVPIPEDITLVSGGVISGL
                     GYTNVHWMLVVSMLGVLVGDSTMYWLGRIYGEKILKFPLIRNVATPERFATVQERFEK
                     QGWKLLFMARFLPGLRAVVYLVSGITRKVSFVKFLLVDFCAAIISVPIWVYLGDYGAS
                     NLDWLKEQVANGQHLIFAILGIVILFLGWKWYKNKKQYKK"
     gene            complement(8055..9074)
                     /gene="purR"
                     /locus_tag="PWX02_001176"
     CDS             complement(8055..9074)
                     /gene="purR"
                     /locus_tag="PWX02_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HDL1729196.1"
                     /translation="MATIKDVAKYAGVSTTTVSHVINKTRFVAEDTTKAVWEAIKALN
                     YSPSAVARSLKINTTKSIGMIITTSESPFFAEIVLAVEEFCYSKGYSLFLCNTQNKLE
                     KIQNHLDMLIKKRVDGILVMCSEYTANSFDIFENSNIPMVIMDWGRSDERSDIILDHG
                     VEGGYIATQHLIENGHQDIAVITGNRDKEIARTRFEGFKKALSEAGLSIREEWIQEGD
                     FEPEGGYECMNNLLKSSEKLPTAVFCFNDSMALGAISAITEKGLNVPQDISVIGYDNV
                     HSSRFYAPPLTTIHQSKSRLGSAALELLLERIEDNENAKEPKVLEFFPELVKRKSVRN
                     LNIKE"
     gene            complement(9210..9938)
                     /gene="cmoA"
                     /locus_tag="PWX02_001177"
     CDS             complement(9210..9938)
                     /gene="cmoA"
                     /locus_tag="PWX02_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HDL1729197.1"
                     /translation="MQRDTLFSAPIEKLGDFTFDESVAEVFSDMIQRSVPGYSNIITA
                     IGMLAERFVTDNSNVYDLGCSRGAGILSIRRNVNAKNVQIIGVDNSQPMVERCKTHLS
                     AYHSEIPVTILLDDIRHIEIKNASMVVLNFTLQFLPREDRLALLQKIYQGLNANGILV
                     LSEKFTFESEEMNELLIDLHHTFKRANGYSELEVSQKRNALENVMRTDSIETHKKRLQ
                     EAGFTQVELWFQCFNFGSMIAIKA"
     gene            complement(9948..10730)
                     /locus_tag="PWX02_001178"
     CDS             complement(9948..10730)
                     /locus_tag="PWX02_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL1729198.1"
                     /translation="MTEKSQLNFNYQPSNNHANAQTMIFLHGLFGDLNNLGAIAKPFA
                     ERYSILMVDLRNHGRSFHSDEMNYALIAQDLAQLLTELNIQNAIVVGHSMGGKSAMAL
                     AAFAPELVEKLVVIDIAPVTYTQNRHQQVMAALFAVKEAKPQNRQQAKAIMAEYIRDE
                     GVQQFMLKSFDAQSEQSFRFNLQALKDNYQNVMGWQQVFVDKPTLFIKGALSDYIQAE
                     HTQTILAQFPQAKSFIVANADHWVHAEKTETVVRAINKFLEK"
     gene            10849..11415
                     /gene="seqA"
                     /locus_tag="PWX02_001179"
     CDS             10849..11415
                     /gene="seqA"
                     /locus_tag="PWX02_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HDL1729199.1"
                     /translation="MKRIEIDDELYQYIASRTQSIGETASDILRRLLRLPQSPQPFVL
                     VQEHMINELKELVKTPSRATKKNESNAEKTVEKLENILNSEHFMNENKNVVRFTMLLA
                     ALYRANPEAFAKATEKVRGNERIYFSQSEAEILATGSGVKAKQIPDSPFWVITNNNTA
                     RKGLILKGVMESMKVPSQLIERIQALFI"
     gene            11425..12843
                     /gene="menE"
                     /locus_tag="PWX02_001180"
     CDS             11425..12843
                     /gene="menE"
                     /locus_tag="PWX02_001180"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDL1729200.1"
                     /translation="MLINSTRFPTQFWAAHSAQNVAIIWEKGTSDYLNHLPSNITWYS
                     LNQLIAQAIFVLQQQGATPHSLIAYCGSHRLIGLLTYCASLALGAKILMLNPAQKASQ
                     RQAVLDDNGVDLIINDADFAKFSAKTTACNAFPTIDFEKPATLTLTSGSSGKPKAVVH
                     SIAAHLYSAEGVCELMAFEQPNSWLLSLPLFHVSGQGIIWRWLFKGATLYVHEGKSDF
                     FLSAQKVSHLSLVPTQLQRYLNQLDSMVSQKCLLGGSMLPAELIAQAQQSGMTTFSGY
                     GMTEMASTICAVENDLNSVGFPLKHRELKLENGEIWVKGKSLGLGYWQKNGQIRPLVN
                     TQGWFATKDRGEWNHKKQLVIKGRLDNMFISGGENIQPEEIEQLIFRSQLVEQIIVLP
                     IEDKQFGHRPVAFLQFKQSDSKNELHKLKIWLSDKLERFKQPVAYYLLDIEKYQKQGL
                     IKLSRTQLQQDLKLKVLKEIYV"
     gene            12836..>13197
                     /locus_tag="PWX02_001181"
     CDS             12836..>13197
                     /locus_tag="PWX02_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HDL1729201.1"
                     /translation="MFRPILKTFVLSLGLMLNSYALAELPTQQAVQTQLDSAKKLDQA
                     DADNKALVQTLEDTLAFLAQIDKQKADNQALNKSIQDSQKALKTSQHNLEKLKEVTIA
                     SVDNLTQKATTDLQKELTS"
BASE COUNT         4239 a         2826 c         2286 g         3846 t
ORIGIN      
        1 gctacattta aacagattga ttagaaaatt caatgccctt tacataaaaa tcttatgctt
       61 tattttcata cattattctg tttaatggta ttctttttta aatagcgata ataggaaaat
      121 caagccggca caaatgacta ccgatggtcc tgccggagta tctttccagc cggatagcaa
      181 taaaccgccg gttactgcaa caatactgaa taaaattgca atcagcacca ttttttcagg
      241 cgttttagca aaacgtcttg cagtagcgga aggaataatc agcagtgagg taataattaa
      301 agcaccgaca aatttcatac ttaatgcgat agttaatgcg gttaaaatca tcagcaataa
      361 acgtaagcga gccacattta atccttcaat ttgagcaagt tccgggctga tggtaatcga
      421 caacaaccgc ttccaaaaca gcattaaaat caaacaaatt agggttgctc cgattccgat
      481 aaacaccaca tcagaaaaat taattgccag taaatcgccg aataagtaag acatcaaatc
      541 cactcgtaca ttatccaaca aactaatggt aattaccccg agagaaaggc tagtatgggc
      601 gataatcccc aacacggtat ccaccgaata attggttttt tgctccaacc aaaccagcaa
      661 taatgcaaga agaatagtca ttccaattac agcaagatac ggatcgagtt ctaaaaaaat
      721 ccctaatgcc acgccgagca atgcagagtg cgaaagtgtg tcgccgaaat acgccatttt
      781 tcgccacacc acaaaagccc ctagaggtgc ggtaataaag gctaacaaaa caccggcaag
      841 ccaagccgga aataaaatat caaacatatt ggttctcaaa ggcaagtaaa acaagcggtt
      901 attttttcgg aaaaatttgc aaattttttg ctgatttaga ccgcttgtca tatttattta
      961 taacgttgaa tcgggctggc ttgatcctgt gctttcagcc acgccatttt ttctttaatc
     1021 tgtttttcca ttccccgctc acttgggaag tagaattcgg tatctttcag ctcaggcggg
     1081 aaataattct ccccggcggc ataagcgttt ggctcattgt gggcatagcg atactcttcg
     1141 ccatagccta aagatttcat taattgagtg ggagcattac gcaaatgcgc cggcacatcg
     1201 tagtcttttg cctctttagc gaggcgcttt gcttcattaa aagcttgata gaccgcattg
     1261 ctctttgggg caactgccaa ataaatgatc gcttgggcaa tcgctctttc accttcataa
     1321 gcccccactc gggtaaagca atcccaagcc acaatcgcaa tttgcattgc tcgagggtcg
     1381 gcattaccaa tatcttcaga agcaatcgca agcagccttc ttgccacata aagcggatca
     1441 ccaccggcgg ttaaaattcg ggcataccag tataatgctc catcagccga tgagcctcga
     1501 atggatttat gtaacgcaga aatgagatca tagtaacgat caccgccttt atcaaaacga
     1561 gcttgtcgtt cgcctaacac ttcggtcagc aaacttttat tcagcacttt ccctttagct
     1621 gaatcttgtg ccatatctac cattaattcc aaacagttca aggcaaaacg ggcatcacca
     1681 ttaacataat cagcaagcaa tgccagtaca tcatcttcaa gtaccaaagt ttcattgcct
     1741 aaaccacgtt cggtatcggc tatcgccatt tgcaaaattt ttagaatttc gaccgcttgt
     1801 aacggcttta aaatataaat tctggctcgg gaaagcagtg cgttatttag ctcaaaagag
     1861 ggattttcgg tagtcgcacc aataaaaata atagtgccgt cctcaatatg cggtaaaaac
     1921 gcatcttgct ggcttttgtt aaagcggtga acttcatcca caaaaagcaa cgttcgtctg
     1981 ccggcttggc ggtttaattt ggcttgttca atcacctctc ggatctcttt tacaccgctg
     2041 gtcacagcgg aaatacgttc aacttccgca tcaagatgat gggcgataat ttcagctagg
     2101 gtcgtttttc cggtgccggg cggtccccaa aaaatcatag aatgactatg tccggcttca
     2161 atcgctctgc gtaacggttt gccttcgcca attaaatgag attgcccgac atattcagcc
     2221 aagtttcgag gtcgcatttt ggctgcaagc ggtcgaaaat cttcactaaa atcaaaagac
     2281 aaactactca ttttaacctc ttaacgtaac cgcagttggc gacgaatatg acgcattaat
     2341 aagaaaatcc aagaccaaag cacaccactg attacagcac ctaacaattc ctgtgaatta
     2401 aattcggcac tgtgtaaaat gtattcaatc acgaaaataa agaaacggat cagcacaata
     2461 tatgccatta ctaataggct ttgtagccat agcgataagt ttcgcagaat cagatggtat
     2521 ttcgcaacaa aataaatcgc caccgataac actaaggcgt ggatgcctaa aatcgagcca
     2581 agaatcagat cccagacgat gccggcaata aaagctgtcc ccacactcat tttatcaggt
     2641 aatgccaatg cccaataaat taacaccaaa acgacccaag tagggcgtag cccttggaat
     2701 cctaccggcc aaggcataat ctcaagtaca aaagcaacca caaaaatcga aattatcaca
     2761 aggatctgaa aaatcgggtt tgttctcatt attccgctcc ttcttccgta tgatagtttt
     2821 gttcattttc cgcttctggt tgtgctgccg gattctcatt tatctcatca ttctctattt
     2881 gttcttcttt tgcctctagc tgcacttcag ttttcttgtt agacatctgt tttaaacgtt
     2941 caaatggatt cacgctatta cgtctttctt caacaacttc acgtacttgc gctggcgaca
     3001 agctttggga gtagtttttc tcttccgccg tcggccaaag cagtaataag taacgtaagc
     3061 gatcaaatga ggctaacgga cgagcaacaa ttctggcaaa ttgacttttg gtatcatttt
     3121 caaccgtttc tacaatcgcc accggatatc cctcagggaa acgtccacct aagcctgaag
     3181 tcaccagcac atcacctttt accacatcaa tagaacgagg caagttatca atgaccaatt
     3241 cattattcac ccctgtacca ctggcaatgc cacgtacatc attgcgtagc acttgaacag
     3301 gaatagcatg agtaatatca gagagcaata aaacacggct agatttttcc cctacagaaa
     3361 taacttgtcc aacaacacct ctttcatcaa taataggttg cccgacaaaa gcaccgtcta
     3421 aacgcccttg attaatcact acttgctgac gataagcatc catctcagca gctaatactt
     3481 cagcaatttt tttatactca tcttgccgaa gcggagaact caataacaat cttaaacgct
     3541 gattttcaac ctttagctga tcaagcaata ataaatcggc acttttttct cttagctgct
     3601 cttttaataa attattctca agctgtaatt tattattatc aataaaattg ccacttaccc
     3661 catctaaaat tgaacggggg gtattagcaa aataatataa tccacttacc gcggtttcta
     3721 atagattacg cgcttgaatc attaaagagc tacgcccatc ggaaataatc agcataatag
     3781 aaaatatcac tgcgataatc aggcgcatcc ctaaagaggg agctttagca aaaataggtt
     3841 tcattagaga aatatatgtg aatttattgg gaatctataa cgaaaattga caatagaatc
     3901 aatccattgc caacctccat ttaaacagct gctaagagaa atgtattaat catcaatact
     3961 aaatacatca ccaccgtgca tatcgatcat ttctaatgct ttaccaccac cacgagctac
     4021 acaagttaat ggatcttctg ctactacgac ttgcacgcca gttgaatcag aaagtaaacg
     4081 atctaaatta tgtaataatg caccacctcc tgttaacacc ataccgcgct caaaaatgtc
     4141 tgctgccaat tcaggtgggc attgctctaa gactgattta actgcctcta caataccatt
     4201 taatggttgt tcaattgctt ctaatacatg tcttgagtta agctcgaaag tacgaggagc
     4261 gccttcagct aaattgtgtc cgtgtacttc cattgtttta acatcatcat cggattcaat
     4321 cttagcacta gcaatttctt gtttaattcg ctctgccgtt gcttcaccaa tagcagaacc
     4381 aaaatgacga cgtacataag caataatagc ttcatctaac ttatcaccac caatacgcac
     4441 agaagttgaa tacacaatgc cgtttaaaga aagcactgcc acttcggttg taccgccacc
     4501 aatgtcaatt accattgatc ccattgcttc gtgaaccggc aaacctgcac cgattgcagc
     4561 cgccattggc tcttcaatta aatagacttc acgcgctcct gctccaattg cagattcttt
     4621 aattgcacgg cgttccactt gtgttgcacc tgctggcacg cacactaata cacgagggct
     4681 tgggcgcata aagttattac tatgtacttg tttaataaag tgttgcaaca ttttttctgt
     4741 tacaaaaaaa tccgcaatga caccatcttt cattggacga atcgccatta tgctttttgg
     4801 tgtacggcct agcattaatt ttgcatgtga gcctaccgcc gcaatacttt ttagcgatcc
     4861 catacgatct tgacgtacag caaccactga gggttcatct agcacaatac cttgaccttt
     4921 aacataaacc agtgtatttg ctgtcccgag atcgatagaa agatcgtttg aaaataatcc
     4981 taaaaatttt ttaaacataa aagaatccgt taaaataaaa ttattcaaaa ataagcacat
     5041 tttatagttc gaacattgct actaatatag caaaaaagcc cgtcattcat caatgaaaaa
     5101 atttaaactc cactagaaaa tttgcagagc aagttgataa gtataaatca gtaaactaaa
     5161 agcgataaag tacataatta gcccggtaac agcatctaaa atttgccaag ttcttcgctt
     5221 tgcaaaataa ggggaaagta agcttgctcc ataacctatg ccaaaaaacc aaataaatga
     5281 gcaaagtaat gcacctgcta aaaagtatat tttaccgtta aaatctaatt tcccaccgat
     5341 actgcctaca atcacaaccg tatcaatata aacgtgagga tttaagaatg taatcgcaaa
     5401 ggtaatcagt aaagcctttt ttaagctcgt tgcattctct ccacttgcca caaggtattc
     5461 accactttta aatacgctga taaatgaacg gctaccgtag cttaatagaa aaatggcccc
     5521 taaaagcgaa ataatgaaac ttacaacagg caattccgca attaacgtgc ctaagcctag
     5581 cacacctatc gtcattaaaa atacatcacc taaaaaacag agcaatgcaa tccaaaatat
     5641 gtgttgtttt aaaatccctt gctttaataa aaaggcattt tgtgcgccaa tagagacaat
     5701 taagccaaaa cacactataa atccttgaat gaaaatatcc attttttctc caaaatcgca
     5761 taacaagcgg tcaattatac agaaaatttt gcaaaacttc ggtataattt atcgaatact
     5821 cttttgactg aataggaaat tttttatgac tgatactagc cagacttcat cttgcgttgt
     5881 cattgcgatt gccggcgcat ctgcctcagg taagagcctg attgcctcaa ccatctataa
     5941 agaactcaaa aatgaattgg gaacagataa tatcggtatt atttccgaag atgcttatta
     6001 tagagatcaa tctcatctaa caatggaaga acggattaaa accaattacg atcaccctaa
     6061 ttcaatggat catcatttat tggttgaaca tttacgccaa ttaaaacaag gtaatgcgat
     6121 tgaaattcca gaatatgact ataccgaaca taatcgtaaa acgaccacga agcactttga
     6181 accaaagaaa atcattattt tagaaggtat tttattgctg acagatgaga atattcgcaa
     6241 tgaaattaat gtttctattt tcgtagatgc tcctctggac atctgcttta tccgccgatt
     6301 acaacgtgat atggtggaac gtggacgttc gatggaatca gtgattagtc aatatcgcaa
     6361 aaccgttcgc ccaatgttct tacaatttat cgaaccctct aaacaatatg ctgatattat
     6421 tgtgccaaaa ggtggcaaaa accgtattgc cattgatatt cttaaagcac aaattaagca
     6481 attactaaaa taaaatagga gctaaatatg cgtttatgtg atacagatat taaacgttac
     6541 ttagatgaag gtattattgc cataacgcct cgaccttcag acgataaaat ttctggcgcc
     6601 actgcagatg tgcgtttagg taattcgttt cgtgtatttc gtgaacataa aacgccctat
     6661 attgatttaa gtggccctcg agaagaagtt gccgcccagt taaataaagt gatgagtgat
     6721 gaaattatca tcggtgatga tgaagcattt ttccttcatc ccggtgacct tgcactcgca
     6781 acaacccttg aatcagtttc cttaccagcc aatattgttg gatggttaga tggtcgttcg
     6841 tcacttgcta gattaggctt aatggtacac gttaccgccc acagaatcga tccgggttgg
     6901 gaaggcaaaa ttgtattgga attttttaat gctggaaaat tacccctagc tttacgccct
     6961 aatatggcaa ttggcgcatt aagctttgag attttaagtg gtgatgctgc aaaaccctat
     7021 aatgcgcgaa aagatgcaaa atataagaac cagcaaagtg ccatttcaag tcggattaat
     7081 caagattaat atagtacatg ccttgcaatc atacattaac tatgttagtt ttttaatcag
     7141 tttattacac agtaaggagt ataactatgt tacattatgc agtcgtattt tttgtcatcg
     7201 ctataatcgc agcgctatta ggatttggtg gtattgctgg aagtgctact gaaattgcca
     7261 aaattctctt ttttgtcttt ttaatactta ctgttatttc gttcttcttc aagaaaaagt
     7321 gacacagtta aacctaattt tttaagttaa aatatagttc aaaaaaacag ttggtaatgg
     7381 atatttataa ttattcttac caactgtttt gctattatat ctttttaaga tgaaatgctc
     7441 gtcttttgtt tcacttttta tactgctttt tatttttata ccatttccag cctaaaaata
     7501 gaataacaat gcctaaaatg gcaaaaatga ggtgctgccc attagctact tgttctttta
     7561 gccaatccaa attagatgcc ccatagtcgc ctaaataaac ccaaattggc acagagataa
     7621 tagcggcaca gaaatcaact aataaaaatt ttacgaagct gacttttctg gtaatacctg
     7681 aaactaagta aaccaccgcg cgcaagccag gtaaaaaacg agccataaaa agtagtttcc
     7741 atccttgttt ttcaaaacgt tcttgcacgg ttgcaaaacg ttcaggtgtc gcaacattcc
     7801 tgattaatgg aaatttgaga attttttcgc catagattct acctagccag tacattgtgc
     7861 tatcgccaac taatactcct aacatactaa caaccagcat ccaatgcaca ttggtgtagc
     7921 ctaatcctga aataacacca ccggatacta acgtaatatc ttcaggaatc ggcacaccga
     7981 aaccacaggc aagtagcaca agaaaaacag cccaataacc atagctacta aaaaattcaa
     8041 tcaatgtttc catattactc cttaatgttt aaattccgaa ccgatttacg tttgaccaat
     8101 tcaggaaaga actctaatac tttaggctct tttgcgtttt cgttatcttc aatacgttct
     8161 aataataatt ctaatgctgc cgagcctaag cgagattttg actgatggat tgtcgtaaga
     8221 ggcggtgcat aaaagcgtga gctatgcacg ttatcatacc cgatcactga aatatcttgc
     8281 ggcacattca gccctttttc ggtaatagct gaaattgccc ctagtgccat tgagtcatta
     8341 aaacaaaaaa ccgcggttgg taatttttca ctgctcttaa gcaaattatt catacactca
     8401 taccccccct caggttcaaa atcgccttct tgaatccatt cttcacgaat agacaaaccc
     8461 gcctcagata gcgctttttt aaaaccttca aaacgggttc ttgcaatttc tttatctcta
     8521 ttgccggtaa ttacagcaat atcttgatgc ccgttttcaa ttaaatgctg agtcgcaata
     8581 tagccacctt caacaccgtg atctaaaata atatcactgc gttcatcact tcgtccccaa
     8641 tccataatca ccattgggat attgctgttt tcaaagatat caaatgaatt agctgtatat
     8701 tcggagcaca tcaccaaaat gccatcaacc cgttttttga tgagcatatc taaatgattt
     8761 tgaattttct caagtttgtt ttgagtattg cacaaaaata gtgaataccc tttgctatag
     8821 caaaattcct ctaccgctaa cacaatttca gcgaaaaacg gtgattcgct ggttgtaatg
     8881 atcataccga tagatttagt cgtattaatt ttcaaactcc gcgctacagc actaggagaa
     8941 tagttcagcg ctttaatagc ctcccaaact gcttttgtag tgtcctctgc tacaaagcgg
     9001 gttttgttta taacgtgaga aaccgttgta gtcgatacac cagcatattt tgcgacatct
     9061 tttatcgttg ccataccttt ttcctcctaa ataagtagtg gtgcattata actgattcta
     9121 actgactttt attataaaga aatagataga atgcaagcgg ttgcttttta tcaagaattt
     9181 gcaaaaaatt ttacaaatat caccgcttgt taggctttaa ttgcaatcat tgagccaaaa
     9241 ttaaagcatt gaaaccaaag ttcaacttgt gtaaaaccag cttcttgcag acgtttttta
     9301 tgggtttcaa tactatctgt tcgcatcaca ttttccaaag cattacgctt ttggctgact
     9361 tccaattcac tgtagccatt tgctcgtttg aatgtgtgat gaaggtcgat caatagctcg
     9421 ttcatttcct cactctcaaa ggtaaatttt tccgataaca ctaaaatgcc attggcattt
     9481 aatccttgat aaatcttctg taataatgct aggcgatctt ctcttggtaa aaattgcaag
     9541 gtgaaattaa gtaccaccat tgaagcattt ttaatttcaa tgtggcgaat atcatcaagt
     9601 aaaatcgtca ccgggatctc agagtgataa gcacttaaat gggttttaca acgctcaacc
     9661 atcggctgag aattatctac cccgataatc tgcacattct tcgcattcac attacggcga
     9721 atagataaaa tccctgcgcc tcgtgaacaa cctaaatcat aaacatttga attatctgtg
     9781 acaaaacgct ccgccaacat tccaatagca gtaataatat tggaatagcc cggcacggag
     9841 cgttggatca tatccgaaaa cacttcagca accgattcat caaaagtaaa atcgcccaat
     9901 ttctcaatcg gagcggaaaa taatgtatct ctttgcattt ttctccctta tttttctaaa
     9961 aatttattga ttgctctcac aacagtttct gttttttctg cgtgaaccca gtgatcagcg
    10021 tttgccacaa tgaaagattt ggcctgcgga aattgtgcca aaatcgtttg tgtatgctct
    10081 gcttgaatat aatccgataa tgcaccttta ataaacaagg tcggtttatc cacaaatacc
    10141 tgctgccacc ccatcacatt ctgataatta tcttttagcg cctgtaaatt aaaacggaaa
    10201 gattgctcgc tttgggcatc aaaagatttc aacataaact gttgcacgcc ttcatctcga
    10261 atatattcag ccattatcgc tttagcttgc tgacgattct ggggtttagc ttctttcacc
    10321 gcaaataagg ctgccataac ctgctgatgc cgattttggg tataagtgac cggggcaata
    10381 tcaatcacta ccaatttctc caccagttca ggagcaaatg cagccaatgc catcgcactt
    10441 tttccgccca ttgaatgccc aaccacaata gcattttgga tatttaattc tgtgagtaat
    10501 tgtgctaaat cttgtgcaat caaagcataa ttcatttcgt ctgaatgaaa agatcgcccg
    10561 tgatttctga gatcaaccat taaaatggag taacgttctg cgaacggttt tgctattgcg
    10621 cctaggttat ttaaatctcc aaacaatcca tgtaagaaga tcatcgtctg agcattagca
    10681 tgattattcg aaggttggta attaaagttt aactgcgatt tttctgtcat ataaatgtca
    10741 ttgttcatat aaattttcag cgatgatcct ctataaaaaa caatagaaat gcgtataata
    10801 gcaaacacta ttaacgcatt taatacccgc agatatttga ggatcccgat gaaacgaatc
    10861 gaaattgatg atgaattata tcagtatatt gcaagccgta cacaatctat tggtgaaact
    10921 gcgtcagata tattacgccg tctattacgc ctgccacaat cgcctcagcc ttttgtttta
    10981 gtacaagaac acatgatcaa cgagcttaaa gagcttgtca aaacgccttc tcgtgctacg
    11041 aaaaaaaatg aatccaatgc agaaaaaacc gttgaaaaat tggaaaacat tttaaattcc
    11101 gagcatttta tgaacgaaaa taaaaatgta gtgcgtttta ctatgctatt agccgcatta
    11161 tatcgagcaa atccggaggc ttttgctaaa gcaaccgaaa aagtgcgtgg taatgaacgt
    11221 atctactttt cgcaaagtga agccgaaatt ttagctactg ggagtggagt aaaagccaag
    11281 cagatcccag attcaccgtt ttgggtaatt accaataata ataccgcacg caagggactt
    11341 attctcaaag gcgtaatgga atccatgaaa gtaccaagcc agttaatcga acgcattcaa
    11401 gcgcttttta tctaattaat tgaaatgctc atcaattcta ctcgctttcc tacccaattt
    11461 tgggctgctc attcagccca aaatgtggcg attatttggg aaaaaggaac atcagactat
    11521 cttaaccacc ttccatcaaa catcacttgg tatagtttga atcagttgat tgcgcaagct
    11581 attttcgtgt tgcaacaaca aggtgctact ccgcacagtt tgattgccta ttgtggttct
    11641 catcggctca ttggcttgct cacttattgt gctagccttg ctttgggggc taagattcta
    11701 atgcttaacc ctgctcaaaa agcaagtcaa cgacaagcag tcttagatga caatggtgtt
    11761 gatctcatta ttaatgatgc cgactttgca aaattttcag caaaaacgac cgcttgtaat
    11821 gccttcccta cgattgattt tgaaaaacca gctaccctaa ccctaacctc aggctcaagc
    11881 ggtaaaccta aagcagtggt gcattctata gcggcccatc tctatagtgc cgaaggagta
    11941 tgtgaattaa tggcatttga gcagccaaac tcttggctac tgagcttgcc tctgtttcac
    12001 gtttccggac aaggcattat ttggcgttgg ttatttaaag gtgcaacact gtatgttcac
    12061 gaaggcaaaa gtgatttttt tctttcagca caaaaagtga gccatctctc tttagtcccc
    12121 acccaattac aacgctattt aaaccaatta gattctatgg tcagccaaaa atgtttactg
    12181 ggtggatcta tgcttccagc cgaattgatt gctcaagcac aacaatctgg gatgactacc
    12241 ttttcaggct acggaatgac tgagatggca tcaaccattt gtgcagtaga aaatgattta
    12301 aacagtgttg gttttccgtt aaaacatcgg gaactaaaat tagaaaatgg tgaaatttgg
    12361 gtaaaaggca aatcactcgg cttaggctat tggcaaaaaa atgggcaaat tcgaccgctt
    12421 gtaaacacac aaggctggtt tgccactaaa gatcgtggcg aatggaatca caaaaaacag
    12481 cttgtaataa aaggacgatt ggataatatg tttatttcag gaggggaaaa tatccaacct
    12541 gaggaaattg aacaactgat ttttcgttct cagcttgtcg aacagattat cgtgttgccg
    12601 attgaagata agcaatttgg acatcgacca gtggcgttct tacaattcaa gcaatctgat
    12661 tcaaaaaatg aactccataa attaaaaatt tggctgtctg ataaattaga aagattcaaa
    12721 cagcctgttg cctattattt actggatata gaaaaatacc aaaaacaagg cttaattaaa
    12781 ctatcaagaa cacaattaca acaagattta aaactcaaag tattaaagga aatctatgtt
    12841 tagaccgatt ttaaaaacat ttgtgcttag tttaggctta atgctaaata gttatgcatt
    12901 agctgaattg ccaacccaac aggcagtgca aacgcagtta gatagcgcca aaaaattaga
    12961 tcaagccgat gcggataata aggcattggt tcaaacactt gaagatacgc ttgctttctt
    13021 agcccaaatt gataaacaaa aagcagataa tcaggcattg aataaatcta ttcaagattc
    13081 ccaaaaagca ttaaaaacaa gccaacataa tcttgaaaaa ttaaaagagg tgactattgc
    13141 aagtgtcgat aatttaacac aaaaagcgac aacagattta caaaaagagt taacctc
//
LOCUS       DANJWC010000056        13187 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.056, whole genome shotgun sequence.
ACCESSION   DANJWC010000056 DANJWC010000000
VERSION     DANJWC010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13187
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.056"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(1..1085)
                     /locus_tag="PWX02_001182"
                     /pseudo
     CDS             complement(1..1085)
                     /locus_tag="PWX02_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249111.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1441..1737
                     /locus_tag="PWX02_001183"
     CDS             1441..1737
                     /locus_tag="PWX02_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HDL1729202.1"
                     /translation="MTAITIPQFEEVTSKQFQSEFGRIVDIVKGKTPVAITQYGRPTM
                     VLFPYDQAVEARRLLAKQRLLEMLAERDKNPPAALAEITLEELDKMIDEERNAL"
     gene            1727..2143
                     /locus_tag="PWX02_001184"
     CDS             1727..2143
                     /locus_tag="PWX02_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HDL1729203.1"
                     /translation="MHSDYLVIDTNILISGLLFPKSNPNLAVKKALLGYTICVSQATF
                     DEFLEVSARPKFAKYFSIQEREEFIANLLDAVKFMEIKETITDCQDPKDNKFLEVAIS
                     AKAKYLVTGDKKELLSMNPYRNVEIISVSEFLAETR"
     gene            2143..3801
                     /locus_tag="PWX02_001185"
     CDS             2143..3801
                     /locus_tag="PWX02_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HDL1729204.1"
                     /translation="MKRFLTLFLLLFSSFSLAETHQIGILAQRGEAYTRTHWQPWVDW
                     LNSQFPNERFELVPLGINEASRRAELDFLLTNQAQFFYLSNQNVRWLATLNSPFSANG
                     EQGAVGSSIWVRADSHYQQLSDLKNQTISAVDNDAFGGFLLGLYQFHQAGMRQNRDFS
                     VQFSGFPVENALALLAEKQVEAAIVPVCLLEELEKEGKFKRSDFRLILQNPQAQGCLA
                     STPLLPNWSLAAMENVPNELAVQLATRLLNSHNPDLPRWTLPFSSAQADHILRELYRH
                     PQQKSLWATVLDWVRLNKFGLLAVVLFILLNLVALRYQVYRKSKALQQAHRKMQQYQQ
                     ELTRADRLALLGEMTTGFAHELKQPLSAVRMYAEGLKSQNSDPYQQRILDKLIAQVDR
                     AVKTMQSIRDWAQNRPSGEPQAVILNQLITNVIEFVAVENRQNAQISLIADRTFRLNL
                     HATVLEQVLTNCLLNALQAGASEISVRLQAVENGLEIAIEDNGGGFSPAQLEFPFVPF
                     RTDKPHGLGLGLVLCQRLMQSLNGRIVLANGENGAKVVLLLPQI"
     gene            3887..4471
                     /locus_tag="PWX02_001186"
     CDS             3887..4471
                     /locus_tag="PWX02_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1729205.1"
                     /translation="MIIHILDDEESILDAMSFLLAPLGVEIQTWQSSLDFLAQADLHR
                     QGVLLLDIRMPLPDGQQVHQQLREAQSTLAVVIMTAHGDVPMAVAELKKGAVDFLQKP
                     ASFEQLKQAIAQAEKVSEKAVKIREISQNYAKLTEKERNLVPLIMQGFTNKQIADKLA
                     ISVRTVEVHRANVMEKMQAESLAGLVQMIGELQY"
     gene            complement(4519..5457)
                     /gene="ttcA"
                     /locus_tag="PWX02_001187"
     CDS             complement(4519..5457)
                     /gene="ttcA"
                     /locus_tag="PWX02_001187"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDL1729206.1"
                     /translation="MTDDIQKSDKKITYNFNKLQKRLRRNVGNAIADFGMIEEGDRVM
                     VCLSGGKDSYTLLDILLNLQQSAPVNFSVVAVNLDQKQPGFPEEVLPRYLESIGVEYK
                     IVEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDMLATLF
                     LNMFYGGKMKSMPPKLISDDGKQIVIRPLAYCKEKDIIKYAEAKQFPIIPCNLCGSQP
                     NLQRQVVKEMLNTWDRQYPGRLETMFSALQDIVPSHLCDPKLFDFKGIKHGQTIEGIE
                     GDTAFDDVKIEAKTFLDEDDDNNFAEAGMIQFKEVN"
     gene            complement(5520..6233)
                     /gene="ubiG"
                     /locus_tag="PWX02_001188"
     CDS             complement(5520..6233)
                     /gene="ubiG"
                     /locus_tag="PWX02_001188"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HDL1729207.1"
                     /translation="MQNVDQQEITKFENMAKTWWDPNGSFKPIHLLNPLRLAYILEKS
                     NGLFGKKVLDVGCGGGILSEAMAKQGAIVTGIDMTSQPLEVAKQHAKESGLEIDYQQT
                     TIENFLAKMTACNQEKFDVITCMEMLEHVPDPLSIIQSCKALLKPDGVLFFSTINRTF
                     KAYMLVIIGAEYVLKMLPKGTHEFEKFIKPAELLAWCDQADLRCQEMRGYHFNPLTEK
                     FWLNNDVSCNYIATLKPNN"
     gene            6298..6678
                     /locus_tag="PWX02_001189"
     CDS             6298..6678
                     /locus_tag="PWX02_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HDL1729208.1"
                     /translation="MTRINLVPPTELCDQHLLAEHRELTRIPNAVAKGKFSLKGQPED
                     YKLGEGHVRFFFNKLTFLKKRYDALHEECLARGFKVQYFWAEHLPQDPSLWLDYQPTE
                     TALTVNRERIALRMPANPRFSERK"
     gene            6775..9471
                     /gene="gyrA"
                     /locus_tag="PWX02_001190"
     CDS             6775..9471
                     /gene="gyrA"
                     /locus_tag="PWX02_001190"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HDL1729209.1"
                     /translation="MSELAKDIIPVSIEDELKTSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFSMDQNSNTYNKPHVKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDAPAAMRYTEVRMQKITQELLTDLDKETVDFSPNYDGKEMIPDVLP
                     TKIPALLVNGSSGIAVGMATNIPPHNLGEVLDGCLAYIENEQISVEELMRYIPGPDFP
                     TGAIINGRKGIEEAYRTGRGKVYVRAKASVETTDKGREQIVVTELPYQVNKAKLIEKI
                     AELVKEKKIEGISKIDDYSDKKGISIVIEVKRDAVGEVVLNHLYSLTQMQVTFGINMV
                     ALDNGQPKVLNLRQIIEAFVKHRREVVTRRTIFELRKARERAHILEGLAIALANIDPV
                     IELIRASKTAEEAREGLLARPWALGNVAPMLEAAGVGASRPEDLPEHLGVRDGEYYLS
                     EAQARAILELRLHRLTGLEHEKIVEEYKELLTEIGELIHILTSPERLMEVIREELERV
                     KTDFNDERRTEITAASGDINIEDLIAQEDVVVTLSHAGYVKYQPLSDYEAQRRGGKGK
                     SATKMKEDDFIEKLLVANTHDTILCFSSRGRLYQLKVYQLPQASRGARGTPIVNILPL
                     VKEENERITAILPIPNGEFSADKFIFMATASGVVKKVSLDAFSNVRASGLIALKLREG
                     DELIGVDITDGNSEIMLFSAQGRVVRFAETAVRAMGRTATGVRGIKLSTTEISSEEIE
                     EAEIIETDNEEAEDSSSVSLDKDRVVSLVIPRTEGDILTVTQNGYGKRTVISEYPVKS
                     RATKGVVSIKVNERNGKVVAAVQVEETDQIMLITDAGTLVRTRVAEVSLVGRNTQGVR
                     IIRTAEDEQVVSLERVCEPEDDENESLDALENAETVETSTEE"
     gene            9567..11114
                     /locus_tag="PWX02_001191"
     CDS             9567..11114
                     /locus_tag="PWX02_001191"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDL1729210.1"
                     /translation="MAAIQQRAELHRQIWQIANEVRGAVDGWDFKQYVLGSLFYRFIS
                     ENFSAYIEQGDESIDYAAYSDEEIDAFGIKEDAIKSKGYFIYPSQLFKNVVKNANTNN
                     NLNIELADIFADIEASAVGFDSEKDIKGLFADFDTKSNRLGNTVEDKNKRLAAVLKGV
                     ESLNFGDFAENQIDLFGDAYEYLISNYASNAGKSGGEFFTPQNVSKLIAQLALYGQSA
                     VNKIYDPACGSGSLLLQAKKQFDDHLIEEGFFGQEINHTTYNLARMNMFLHNINYDKF
                     HIELGDTLINPKLKDDKPFDAIVSNPPYSIKWIGSDDPTLINDERFAPAGILAPKSKA
                     DFAFILHALNYLSAKGRAAIVTFPGIFYRSGAEQKIRQYLIEQNVVESVIALSANLFY
                     GTSIATNILVLSKHKTDTKTQFIDASDLFKKETNNNVLTDEHIAQILKLFADKADVEH
                     FAKSVDNRQIAENEYNLAVSSYVEAKDTREVINITELNKEIRKTVAKIDRLRADIDAI
                     VAEIEQQ"
     gene            11190..12389
                     /locus_tag="PWX02_001192"
     CDS             11190..12389
                     /locus_tag="PWX02_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014668407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL1729211.1"
                     /translation="MSNSIIEKLLNGAEVEWKPLGEVAELKRGKTITAKDKTEGNIPV
                     ISGGQKPAYYTGEYNREGETITIAGSGAYAGFLMYWNEPIFLGDAFSVKPDLDILITK
                     YVYHFLLSKQQWIFNLKKGSGVPHVYPKDLAILEIPIPPLEIQQKIVKTLDKFTELEA
                     TLEAELALRKKQYQYYRETLLTFPQDLDRGGYNDITKAIGQVVGKNAHPTVVWKSLGE
                     IGEARMCKRILKEQTSNVGDIPFYKIGTFGKKPNAYISRELFEDYKQKYSYPRKGNIL
                     ISASGTIGRTVIFDGEDSYFQDSNIVWIENDESQVLDKFLFYLYQIADWNIAEGGTIQ
                     RLYNDNLKKLKIPVPPLSEQQKIVNILDKFDSLTNSITEGLPKEIKLRREQYGYYREQ
                     LLNFPKP"
     gene            12399..>13187
                     /locus_tag="PWX02_001193"
     CDS             12399..>13187
                     /locus_tag="PWX02_001193"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anticodon nuclease"
                     /protein_id="HDL1729212.1"
                     /translation="MSKILDDIALELRNNKKKVQLIYAFNGTGKTRISRKFKELISPK
                     LEDDTEYSGELKHKKILYYSSFTEDLFYWDNDLEYDSKIKLKIHKNSFTDWVFIEQGL
                     EDTVTRIFQHYTNEKLTPNFNEEYTFQNEYGDNITIPAYSEIVFSYARGDDAFQENIK
                     ISKGEESNFVWSVFYSLFEQVISELNIAEIKNRSTDKFNNLEYVFIDDPVSSLDENHF
                     IELAIDIAQLIQSSESNLKFIITTHNPLFYNVLHNEFRRENSISY"
BASE COUNT         4034 a         2539 c         2872 g         3742 t
ORIGIN      
        1 ttttgagggg atgtgtacat ccctttatta gttaaatatt tcttaattaa atcaaactct
       61 ctcgtaggat agttttggac atctcctcca tttttataaa agtgaatttc tgataagtgt
      121 gatagctctc ttctaaatcc ttgatattca aaataaaacc atcttttaaa atgatattca
      181 tcatcaaagt atggtatgtc agagcgtttt aaaaatgggg aatatatttg gtgttcaaaa
      241 agttcacttc tctgattttt atatacaaaa tcatattcac cattttctcc tttaagaaag
      301 accaaattat cattataggg tattacatct acccatcctt tatctaaaaa ttaaattctt
      361 gatacaaaaa aatctaaaaa tttcttatgt agagctaagt cattgttaat atccccttga
      421 taaccttgag ggtaataaca ttgtatttca tatttatcca atctaggctg gactaactga
      481 tcacctatca agttaaaata ataaaacaat actttatcaa gatatctacc tatgactaca
      541 tctgccatac ttctgattgt gccatcacgg caatcaattt ttattgatgg attttttaat
      601 gcttcattta tgtcttcaca tatacctatg tcagaatatg gggtttttaa ggaatttccg
      661 atacgaaaac cattagctaa atgcatatca tatcggaaat taaatccagt aaatattctc
      721 ttagggattt cctttccgct aaagaaaatt ctttcagata tatcttgctt tttcagagct
      781 aagctccttg tcatgcacat atcatttact ggatattttt gttttgttaa atctacaaga
      841 tataagagcc catgtttttc catgaattgc gtcaaattag ataaataatc ttcttgaaac
      901 cctgcaaagt ctccagaaaa tcctaaagct tttgagcaag catttaacaa agtacagtga
      961 gaaatttttt ctctatctct agaacaatct ttaacagatt ttttaattaa ttctggggaa
     1021 ataccataaa ttggtagaat atggtatttt tgtaaaggta aatagctatg ggtgtttttt
     1081 gacatatatt aaatcctctt tgttaattaa gttagaaaaa tgcctctgtg aaaatcacag
     1141 ccatactagg aaataaaagg atttaattta taaatgaatt gactataagc aatataatct
     1201 atcgaattat ctcttttgtt caaggcaagt atggatacct gaccgcaggc ctggagatag
     1261 aacctacata taatatgccc tactacatta agccataaaa aatagaaaat caagtgtttg
     1321 ttcaagtttt tatgttattg aatttacaat aactacaaaa atttatctta attggctcga
     1381 ttgattttta cacaaaacat agctaacata tccaaaatca ctcacattag gagcttaaaa
     1441 atgacagcga ttactatccc tcaatttgaa gaggtgactt ctaagcaatt tcaatctgag
     1501 ttcggcagaa ttgttgatat cgtaaaaggc aaaacccctg tggcgattac ccaatatggc
     1561 agaccgacaa tggtgctttt tccttacgat caagcggttg aagctcggcg attattagca
     1621 aaacagcgtt tgcttgaaat gcttgcagag cgagataaga atccgccggc agctcttgca
     1681 gaaatcacgc ttgaagagtt ggataaaatg attgatgagg aacgtaatgc actctgatta
     1741 tttagtgatt gatacgaata ttttgattag tggtttattg ttcccaaaat caaatcccaa
     1801 tttagcagtg aaaaaagcct tattaggcta taccatttgt gtctcgcaag cgacttttga
     1861 cgaattttta gaagtctccg ctagaccgaa atttgctaaa tatttcagta ttcaggaaag
     1921 ggaagaattt attgccaatt tgttggatgc agtaaagttt atggaaatta aagaaacgat
     1981 tacagattgc caagatccca aagataataa atttttagaa gtggcgatca gtgcaaaagc
     2041 gaagtattta gtgacgggcg ataaaaaaga gctcctcagt atgaaccctt atcggaatgt
     2101 tgaaattatt tctgtcagcg aatttttagc ggaaactcgc taatgaaacg gtttctcact
     2161 ctttttcttc tccttttttc atctttttca ttagccgaaa cccaccaaat cggcattctc
     2221 gctcagcgtg gggaagccta cacccgcacc cattggcagc cttgggtgga ttggttaaat
     2281 tcgcagtttc ccaacgagcg gtttgagctg gttccattgg ggattaacga ggcgagccgc
     2341 cgggcggagt tggattttct gctgaccaat caagctcagt ttttctattt gagtaaccaa
     2401 aatgtgcgat ggcttgccac gctcaacagt ccgttttcgg caaatggtga gcaaggggcg
     2461 gtgggcagta gcatttgggt gcgtgccgac agccattatc agcagttaag cgatctgaaa
     2521 aatcagacta tcagtgcggt ggataacgat gctttcggcg gttttttgct tgggttgtat
     2581 cagtttcatc aagcaggaat gcggcaaaat cgggattttt cggtgcagtt tagcggtttt
     2641 ccggtggaaa atgcgttggc tttgctggcg gaaaaacagg tggaagcggc gattgtaccg
     2701 gtctgtttgc tggaagagtt ggagaaagag ggcaagttca agcggtcgga ttttcgctta
     2761 attttgcaaa acccacaggc gcagggttgc cttgccagca cgccgttgtt gccgaactgg
     2821 tcgctggcgg caatggaaaa tgtgccaaac gagctggcgg tacagcttgc cacacgcttg
     2881 ctaaacagcc acaatcccga cctaccccgc tggacgttgc cgttttcttc cgcccaagcc
     2941 gatcacattc tgcgtgaact gtatcgccac ccgcaacaaa aaagcctgtg ggcaacggtg
     3001 ctggattggg ttcgtctcaa taaattcggc ttgttggcgg tggtgttgtt cattttgctc
     3061 aaccttgtcg ccctgcgtta tcaggtctat cgcaaaagca aggcgttaca gcaggcacac
     3121 cgcaaaatgc agcagtatca gcaggaactg acccgagccg accgccttgc tttgttgggc
     3181 gagatgacaa ccggttttgc tcacgaactg aaacaaccgt tgtcggcggt gcgaatgtac
     3241 gccgaggggc tgaaaagcca aaacagcgat ccttatcaac agcggatttt ggataagctg
     3301 attgcgcagg tagatcgggc agtgaaaacg atgcagagca ttcgggactg ggcgcaaaac
     3361 cgcccgagtg gtgaaccgca agcggtcatt ttgaaccaat tgattactaa tgtgatcgag
     3421 tttgtggcgg tggaaaatcg ccagaatgca caaatctcgc tgattgccga ccgcactttt
     3481 cggcttaatt tacacgccac ggtactggaa caagtactga cgaactgcct gctcaacgcc
     3541 ctgcaagccg gtgcaagcga gatatcggtg cggttacaag cggtcgaaaa cgggctggaa
     3601 attgcaatcg aagacaacgg cggcggtttt tcccctgccc agcttgaatt tccgtttgtg
     3661 ccatttcgca ccgacaaacc acacggctta gggctaggct tggtgctatg ccaacggctg
     3721 atgcaatcgc tcaacggacg gattgtattg gcgaacgggg agaacggggc gaaggtagtg
     3781 cttttactcc ctcaaatata aatgtagggg caggtcctgt gcctgcccgt aaatcctatc
     3841 aaattcaggg cgggcacagg acccgcccct acggaaaaaa gaaaaaatga ttattcacat
     3901 tcttgatgac gaagaaagca ttttagacgc tatgtcgttc ctgcttgccc cgcttggggt
     3961 ggaaatccaa acgtggcaat ccagcctaga ttttttggca caggcggatt tacaccggca
     4021 aggcgtgctg ttactagata ttcgaatgcc gctgccagac gggcaacagg ttcatcaaca
     4081 actgcgagag gcacaatcga ccctggcggt ggtgattatg accgcccacg gtgatgtgcc
     4141 gatggcagtc gctgaattga aaaaaggcgc agtggatttt ctgcaaaagc cggcaagttt
     4201 tgaacagcta aaacaggcga ttgcacaggc ggaaaaagta agtgaaaaag cggtgaaaat
     4261 tcgtgaaatt tcgcaaaatt acgccaaatt gacggaaaaa gagcgtaacc ttgtgccact
     4321 gattatgcaa ggtttcacca ataaacagat cgcagataaa ttagcgattt cagtgcgtac
     4381 ggtggaagta catcgagcca atgtgatgga aaaaatgcag gcggagagtt tggcggggct
     4441 ggtgcagatg atcggggagt tacaatatta aaggtagcgg aaattgctct gctaccttta
     4501 gtttcgactt ttgccgtatt aatttacttc tttaaactga atcattcccg cttcggcaaa
     4561 attgttatcg tcatcttcgt ctaagaaggt tttggcttca attttgacat catcaaaagc
     4621 ggtgtcgcct tctatgcctt caatggtttg accgtgttta atccctttaa aatcaaacaa
     4681 tttcggatcg cataagtggg atggcacgat atcttgcaaa gcactgaaca ttgtttctaa
     4741 acgccccggg tattggcgat cccaagtatt cagcatttct ttcaccactt ggcgttgtaa
     4801 attaggctga gaaccacaca gattacacgg aataatcggg aactgcttgg cttcggcata
     4861 tttgataatg tctttttctt tacaataggc gagtgggcgg atcacaattt gcttgccgtc
     4921 atcagagatc agtttaggtg gcatactttt cattttgccg ccgtagaaca tatttaagaa
     4981 taaagtcgcc agcatatcgt cacggtgatg ccctaatgca attttggttg cacccaattc
     5041 ggtggcggtg cggtataaaa tcccacggcg aagtcgtgag caaagtgaac aggtggtttt
     5101 gccctccgga attttctctt ttacgatgcc gtaggtgttt tcttccacaa ttttgtattc
     5161 cacgccaatg ctttctaaat agcgaggcaa tacctcttcc ggaaatcccg gttgcttttg
     5221 gtctaaattg accgctacca cactaaaatt caccggtgca ctttgctgta aattgagcaa
     5281 aatatctaac agcgtatagc tgtctttacc gccggaaagg cagaccatta cacggtcgcc
     5341 ctcttcaatc ataccaaaat cggcaatggc attaccgaca ttacggcgta aacgtttttg
     5401 taacttgttg aaattataag taattttttt atcagatttt tgaatatcgt ctgtcataat
     5461 agttgtacta ggcttagaaa ttagcggtgt atagtaaaga tttcgcacta ttttgcaagt
     5521 taattattcg gcttcagcgt agcgatatag ttacaactca catcattatt gagccaaaat
     5581 ttttcggtta atgggttgaa atggtagcct ctcatctctt ggcaacgtaa atcagcctga
     5641 tcgcaccaag ctaacaattc ggcaggcttg atgaattttt caaattcgtg cgtgcctttt
     5701 ggcaacattt tcagcacata ttccgccccg ataattacta acatataggc tttaaaagta
     5761 cggttgatgg tagagaaaaa cagcacgccg tcaggtttga gcagggcttt acagctttga
     5821 atgatagaaa gcggatcggg aacgtgttcc agcatttcca tacaagtaat cacatcgaat
     5881 ttttcttgat tacaagcggt catttttgct aaaaaatttt caatcgtggt ttgttggtaa
     5941 tcaatctcta acccactttc tttggcgtgc tgctttgcca cttcaagcgg ttgagaggtc
     6001 atatcgatgc cggtaacaat cgctccctgt tttgccattg cctcacttaa aataccgcca
     6061 ccacagccga catctaacac ctttttgcca aataaaccgt tcgatttttc taaaatatag
     6121 gctaaacgca gtggattaag cagatgaatc ggcttgaaac tgccgtttgg atcccaccag
     6181 gttttagcca tattttcaaa ttttgtgatt tcttgctgat cgacgttttg cataaactgt
     6241 tcctatggta aatttagggc attactataa cgcatttagc ctaaaaggaa aatcagaatg
     6301 acccgaatta accttgttcc gccaacggaa ttatgcgatc aacatctgct tgccgaacat
     6361 cgtgaactta ctcggattcc gaatgcggta gcgaaaggca aatttagcct aaaaggacag
     6421 ccagaagatt acaaactggg cgagggacac gttcggtttt tcttcaataa actcacgttt
     6481 ttgaaaaagc gttacgatgc gttacacgag gaatgtttag caagaggttt taaggtgcaa
     6541 tatttctggg cggaacattt accacaagat ccctctctct ggctagatta tcagccgact
     6601 gaaaccgctc tgactgtaaa tcgggagcga attgcattaa gaatgccggc aaatccacgt
     6661 tttagcgaaa gaaaatgacc gcttgtacgc ctttttgttg ctttttatgc tataatctcg
     6721 ccaatttttt cgccttattt aaggctcttt tactaataaa attaggaaat ttcaatgagc
     6781 gaattagcca aagatattat tcccgtaagt attgaggacg aattaaaaac ctcttacctt
     6841 gactatgcga tgtcggtgat tgttgggcgt gccttgcctg atgtacgaga cggtttaaag
     6901 ccggtacacc gccgagtgct tttttcaatg gatcaaaaca gcaacaccta taataagccg
     6961 cacgttaaat ctgcccgtgt ggtcggtgat gtgatcggta aatatcaccc acacggtgac
     7021 tctgcggtgt atgacactat tgtgcgtatg gctcaaccgt tctcactacg ttatatgttg
     7081 gtggacgggc aaggtaactt tggttctatt gatggcgatg caccggcagc aatgcgttat
     7141 accgaagtgc gtatgcagaa aattacgcag gaattattaa ccgatctcga taaagaaacg
     7201 gtggatttct caccgaacta tgacggcaaa gagatgattc ctgatgtatt gccaaccaaa
     7261 attccggctc tgttagtcaa tggttcttca gggattgcgg tcggtatggc aaccaatatt
     7321 ccacctcata atttaggcga agtattagat ggttgcttgg cgtatattga aaatgaacag
     7381 attagtgttg aagagctaat gcgttatatc ccggggccgg atttcccgac cggggcgatt
     7441 atcaacggtc gtaaagggat tgaagaggct taccgcaccg gtcgtggcaa ggtgtatgtg
     7501 cgcgcgaaag ccagcgtgga aacaactgat aaagggcgtg agcaaattgt tgtaaccgaa
     7561 ctgccatacc aagtgaataa ggcaaaatta attgaaaaaa ttgccgagct ggttaaagag
     7621 aagaaaatcg aaggcatcag caaaattgat gactattccg ataaaaaagg gatttcgatt
     7681 gttattgaag tcaagcgtga tgcagtgggt gaagtggtgt taaaccacct ttattcttta
     7741 acccaaatgc aggtcacgtt tggtatcaat atggtggcgt tagataatgg gcagccaaaa
     7801 gtgctgaact tacgccagat catcgaagcc tttgttaaac accgtcgtga agtggtcaca
     7861 cgccgtacga tttttgaatt gcgtaaagcc cgtgaacgtg cccatatttt agaaggtttg
     7921 gcgattgcgt tagcgaatat cgacccggta attgaattaa ttcgagcctc aaaaaccgca
     7981 gaagaagccc gtgaaggctt attggctcgc ccttgggcat taggcaatgt tgcaccaatg
     8041 ttagaggctg ctggtgttgg tgcttcccga ccggaagatt tacctgaaca cttaggagtg
     8101 cgtgatggtg aatactattt atctgaagct caagccagag cgattttaga gctacgctta
     8161 caccgcttaa ccggtttaga acacgagaaa atcgttgaag aatacaaaga actcttaact
     8221 gaaattggcg aattaatcca cattttaacc agcccagaac gcttaatgga agtgatccgt
     8281 gaagagttag aacgtgtaaa aaccgatttt aatgatgaac gccgtactga aattaccgca
     8341 gcctctggcg acatcaacat tgaagactta atcgcacaag aagatgtggt ggttacgctt
     8401 tctcacgccg gctatgtgaa atatcagccg ttatccgact acgaagcaca acgccgtggc
     8461 ggtaaaggta agtcggcaac caagatgaaa gaagatgatt tcatcgaaaa actcttggtg
     8521 gcaaacaccc acgacaccat tctttgcttc tcaagccgtg ggcgtttata tcaattaaaa
     8581 gtgtatcaat taccgcaagc aagccgtggg gcaagaggca cgccgattgt gaatattctg
     8641 ccgttagtga aagaagaaaa cgaacgtatt accgcgattc taccgattcc aaacggtgaa
     8701 tttagtgccg ataagtttat ctttatggca acagctagcg gtgtagtgaa aaaagtttcc
     8761 cttgatgcct ttagcaacgt gcgtgcaagc ggtctgattg cgttaaaact tcgtgaaggc
     8821 gatgagctga tcggggtaga tattactgat ggtaacagcg aaattatgtt gttctcggca
     8881 caagggcgag tagttcgctt tgccgaaaca gcagttcgtg cgatgggacg taccgccacc
     8941 ggcgtacgag gtattaaact ttctaccacc gaaatttcaa gcgaagaaat tgaagaagct
     9001 gaaattattg aaactgacaa tgaagaggca gaagattcct ccagcgtcag ccttgataaa
     9061 gaccgtgtgg tttctttagt gattcctcgc accgaaggtg atattttaac cgtcacccaa
     9121 aacggctacg gtaaacgtac ggtgattagc gaatatccgg tgaaatcccg agcaaccaaa
     9181 ggggtcgttt cgattaaagt gaatgaacga aacggcaaag tggttgcggc agttcaagta
     9241 gaggaaaccg accaaattat gttgattacc gatgccggca cgctggttcg cacccgagtg
     9301 gcagaagtca gcctcgttgg acgaaacacc caaggcgtac gtattattcg caccgcagaa
     9361 gacgaacaag ttgttagcct tgaacgagtc tgcgagcctg aagacgatga aaatgaaagc
     9421 ctagacgcat tagaaaatgc cgaaacagta gaaacatcaa ctgaagaata atcttaacta
     9481 aacaagcggt cgaattttca caattttttg caaaaataga aggaaattcg accgctttaa
     9541 tatatgagca atattaggaa tacaaaatgg cagctattca acaaagagca gaactccatc
     9601 gtcaaatttg gcaaattgca aacgaagtgc gtggtgcggt ggacggctgg gattttaaac
     9661 aatatgtatt aggttcgctg ttttatcgtt ttattagcga aaatttttcc gcctatatcg
     9721 aacaaggcga tgaaagtatt gattacgcag catattccga cgaagagatt gatgcattcg
     9781 gtattaaaga agatgcgatt aagagcaaag gctactttat ttacccaagc cagttgttta
     9841 aaaatgtcgt gaaaaatgcc aataccaata acaatttgaa tattgaactc gccgatattt
     9901 ttgccgacat tgaagcttct gcggtagggt ttgattcgga aaaggatatt aaaggcttgt
     9961 ttgccgattt tgataccaaa agtaaccgct tgggtaatac cgttgaagat aaaaacaagc
    10021 gattagcggc ggtattgaaa ggggtagagt cgcttaattt tggtgatttt gccgagaacc
    10081 agatcgacct gttcggcgat gcctatgaat atctaatttc taattatgcg tctaacgcag
    10141 gtaaatcggg cggtgaattt ttcacgccac aaaatgtgtc taaactcatt gcacaacttg
    10201 ctttatacgg gcaaagtgcg gtcaataaaa tctatgatcc ggcttgtggt tccggctcac
    10261 tgttattgca agcgaaaaag cagtttgacg atcatttgat tgaagaaggc tttttcgggc
    10321 aggaaatcaa ccacacgact tacaacctcg cccgaatgaa tatgttcttg cataatatta
    10381 actatgacaa attccatatt gagctgggcg acacactgat taatccaaaa ctcaaagatg
    10441 acaaaccttt tgatgcgatt gtctctaacc caccttattc aattaaatgg attggctcgg
    10501 acgaccctac actcatcaac gatgagcgtt ttgctcctgc cggcattctt gctccgaaat
    10561 ccaaagccga ttttgccttt attctgcacg ccctgaacta cctttcagca aaaggacggg
    10621 cggcgattgt tactttcccc ggcattttct accgtagtgg tgcggagcaa aaaatccgtc
    10681 agtatttaat tgaacagaat gtggtagaaa gtgtgatcgc cttgtcagca aatctctttt
    10741 acggcaccag cattgcgact aatattttag tgctatctaa acacaaaact gacaccaaaa
    10801 cccaatttat tgatgcaagc gatctgttta agaaagaaac taacaataat gtgctgaccg
    10861 atgaacatat cgctcaaatt ctcaaattat ttgccgataa agccgatgtg gagcatttcg
    10921 ccaaatcggt agataaccgc caaattgcgg agaatgagta caatcttgcg gttagctctt
    10981 atgtggaagc aaaagatacc cgagaagtga tcaatatcac cgaactcaat aaagaaattc
    11041 gcaaaacagt cgcaaaaatt gaccgcttgc gggcagatat tgatgcgatt gtggcggaga
    11101 ttgaacaaca gtgatcgtgg ataataaaaa gggcggatca cattcgccca aataaaacaa
    11161 gcggtataaa atcaaggaaa atttgcaaaa tgagtaatag tattattgag aaattattaa
    11221 atggggctga ggtcgagtgg aagccgttag gagaggtcgc agagttaaaa cgcggaaaaa
    11281 caattacagc gaaagataaa acagaaggga acattcctgt aattagtggt ggtcagaagc
    11341 cagcatacta tactggcgaa tataatagag agggtgaaac aattactatt gcaggaagtg
    11401 gggcatatgc tggtttctta atgtattgga atgaaccaat ttttcttgga gatgcttttt
    11461 ccgtaaaacc agatttagat attcttatca caaaatatgt atatcatttt ttgttatcta
    11521 aacagcagtg gatttttaat ttaaaaaaag ggtcaggtgt tccacatgtt tatccaaaag
    11581 atttagctat attggaaatc cccattcctc cactcgaaat tcaacaaaaa attgtaaaaa
    11641 cacttgacaa attcacggag ctggaagcta cgctggaagc tgaattagct ctgcggaaaa
    11701 aacaatatca atattaccga gaaaccctat tgacattccc acaagattta gatagggggg
    11761 ggtataacga catcaccaaa gccatcgggc aagtggtggg caagaatgcc caccctacgg
    11821 tggtttggaa gagtttgggg gagatcggtg aagctagaat gtgtaaaaga attttgaaag
    11881 agcaaactag caatgttgga gatattccgt tttacaaaat tggaacgttt ggaaaaaaac
    11941 cgaatgctta tatttcaaga gaattatttg aagattacaa acagaaatat agttacccta
    12001 gaaaagggaa tatcttaatt tctgcatctg gaacaatagg gagaacagta atttttgatg
    12061 gcgaagattc ttattttcaa gatagtaata ttgtttggat tgaaaatgat gaaagtcagg
    12121 tcttagataa gtttttattt tatctttatc aaattgctga ttggaatata gcagaaggcg
    12181 gaacaattca gaggttgtat aatgataatt taaaaaaatt aaaaattcct gttcctcctc
    12241 tttccgaaca acaaaaaatc gtcaatattt tagataaatt cgacagctta accaattcaa
    12301 tcaccgaggg tttaccaaag gaaattaaat taaggcgaga acagtatggg tattatcgtg
    12361 agcagttatt aaattttcct aagccataag gataacatat gagtaaaatt ttagatgata
    12421 ttgctctaga actaagaaat aataagaaaa aagtacagct catttatgct tttaatggga
    12481 ctgggaaaac aaggatatct cgaaagttta aagagcttat ttctcctaaa ttagaggatg
    12541 atactgagta tagtggagaa ttaaagcata agaaaattct atactattcc tcttttacgg
    12601 aagatttatt ttattgggat aatgatttag agtatgattc taaaattaaa ttaaaaatac
    12661 ataaaaactc cttcactgat tgggttttta tagagcaggg gctagaagat acagtaacta
    12721 ggatttttca gcattatact aatgaaaaac taacccctaa ttttaacgaa gaatatactt
    12781 tccaaaatga gtatggagat aatattacta tcccagccta ttcagaaata gttttctcct
    12841 acgctagagg cgatgatgct tttcaggaaa atattaaaat ttctaaaggg gaggaaagta
    12901 attttgtttg gagtgttttt tattcgctgt ttgagcaagt tatatcagag ttaaatattg
    12961 ctgaaataaa aaatcgttca acagataaat ttaataattt agaatatgtt tttattgatg
    13021 atccagttag ctcactagat gaaaatcatt ttattgaatt ggcaattgat attgcccaat
    13081 taatacaatc aagcgaatca aatcttaaat ttattattac aacacacaat ccactttttt
    13141 ataatgtgtt acataatgaa ttcagaaggg aaaatagtat ttcttat
//
LOCUS       DANJWC010000057        13123 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.057, whole genome shotgun sequence.
ACCESSION   DANJWC010000057 DANJWC010000000
VERSION     DANJWC010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13123
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.057"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            99..440
                     /locus_tag="PWX02_001194"
     CDS             99..440
                     /locus_tag="PWX02_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL1729213.1"
                     /translation="MSSFQNWTEHTAHVKQSFGKLGQKHPKMLEAYKALGTAAAAEAL
                     DHKTRELIALAVAITTRCESCISVHAEEAAKAGATESEIAGALATAIALNAGAAYTYA
                     LRALEAVEEQK"
     gene            451..804
                     /locus_tag="PWX02_001195"
     CDS             451..804
                     /locus_tag="PWX02_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL1729214.1"
                     /translation="MKKLAWILGFAAMMPMMSSAKQASVQQVQAEKAAGIWIDVRSAE
                     EFQQGHLNGAINVTHTEIAQRIAEVAPDKNQPIHLYCRSGHRAEAALNELKKLGYSNV
                     TNHGGYDDLIKAGLK"
     gene            864..1502
                     /locus_tag="PWX02_001196"
     CDS             864..1502
                     /locus_tag="PWX02_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005719747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgB family protein"
                     /protein_id="HDL1729215.1"
                     /translation="MSAFNNFVNFVANIVAPMQRQFINFVRIAICIVMVWIGGLKVCQ
                     YEADGIAHFVSNSPFFSYMYKKGPNLVDDGTGKMVMEYTLHKNPEGKMVAKNIEWHKE
                     NGTYTASYIIGATIVTVGLLTLSGIWFPVSGMAGGLLTFGMSIVTLSFMITTPEIWVP
                     NLGGDMPTPAHGFPYLSAVGRLIVKDVIMMAGGLVAAAECANRILARRKAIA"
     gene            1637..2818
                     /gene="purT"
                     /locus_tag="PWX02_001197"
     CDS             1637..2818
                     /gene="purT"
                     /locus_tag="PWX02_001197"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HDL1729216.1"
                     /translation="MTTIGTPLRTNATKVMMLGSGELGKEVVIELQRLGVEVIAVDRY
                     DNAPAQQVAHRAYTISMLDGNALRDLVEKEKPDFIVPEVEAIATATLVELEQEGYNVI
                     PTAKATQLTMNREGIRRLAAEELGLKTSPYRFVDNFEQFQQAIQEIGIPCVVKPIMSS
                     SGHGQSVIKSEADIQQAWDYSQQGGRAGGGRVIVEGFIKFDYEITQLTVRHIHGTSFL
                     APIGHIQVDGDYRESWQPQQMSGIALKKAQETAEKITSALGGRGIFGVELFVCGDEII
                     FNEVSPRPHDTGIVTMASQELSQFALHARAILGLPIPEIYRISPAASKAIVVEGKSDN
                     VRFGGVDKVLAEIGTNIRLFGKGEVNGHRRLGVILARDENTVRALEKARRAYDKLDIQ
                     L"
     gene            complement(2910..4403)
                     /locus_tag="PWX02_001198"
     CDS             complement(2910..4403)
                     /locus_tag="PWX02_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005556599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL1729217.1"
                     /translation="MFKQLQQVGKAFMLPIAILPAAGLLLGIGGALSNKATVQAYPIL
                     DNPTLQGIFQIMASAGSVVFGNLALLLCIGLGIGLAKRDKGVAALAAVVGYLIMTGTI
                     SALIPLFSPETKGIDTGVIGSLVMGLITVKLHNKYHNIQLPQILGFFGGSRFVPIVTA
                     FAAIFVGAVFFLIWPTFQQWLVSAGQGIAEMGEIGTFFYGFLMRLSGAVGLHHMIYPL
                     FWYTELGGTEVVNGETIIGAQKIFFAQLADPTHQGLFTEGTRFFAGRFDTMMFGLPAA
                     CLAMYHAVPKARRGAISGLFLGAALTSFITGITEPIEFMFLFVAPWLYVFHAFLDGVS
                     FYIADLLNISIGNTFSGGVIDFTLFGILQGNENTNWLRVIIVGLPWAALYYFSFLFLI
                     KKFNVMTPGRAEESEEVVEQQTSSLTENAHKIIAALGSANNIENVDACITRLRVAVKE
                     VKAVDKATLKKLGAIDVLEVGGGVQAVFGAKAVLYKSEVNQILGKED"
     gene            4604..6451
                     /gene="ilvD"
                     /locus_tag="PWX02_001199"
     CDS             4604..6451
                     /gene="ilvD"
                     /locus_tag="PWX02_001199"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012055207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDL1729218.1"
                     /translation="MPILRSATSTQGRNMAGARALWRATGMKENDFGKPIIAVVNSFT
                     QFVPGHVHLKDMGQLVAAEIEKAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRDLIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPTIFVSGGPMEAGKTKLSDQLI
                     RLDLVDAMIESADPNVSDERIDAIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGN
                     GSMLATHADRKELFLRAGREIVELCRKYYQQDDESVLPRSIATFDAFENAMSLDIAMG
                     GSSNTVLHLLATAQEAGVDFKMEDIDRLSRVVPCLSKIAPNTNKYHMEDVHRAGGIMG
                     LLGELDRAGLIHRHVKTVLGLTIEEQLNQYDIIRNKDEDLHKFFRAGPAGIRTTKAFS
                     QDCRWDTVDDDRVNGCIRNVENAVTKEGGLAVLFGNIAEDGCIVKTAGVDESIWKFTG
                     TAIVFESQEDAVAGILGGKVKEGHIVIIRYEGPKGGPGMQEMLYPTSYLKSMGLGKKC
                     ALLTDGRFSGGTSGLSIGHASPEAASGGAIALINDGDTVAIDIPNRSINLMVSDEELA
                     ARRAEMEARGDKAWQPLKREREVSFALKVFGHFATSADKGAVRDKSLLK"
     gene            complement(6585..6935)
                     /locus_tag="PWX02_001200"
     CDS             complement(6585..6935)
                     /locus_tag="PWX02_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HDL1729219.1"
                     /translation="MWHFYLMTIELNNIQYPTDASGYLTNLNDWSVELAEKIAEKEQI
                     SLTAEHWEIIYLVRDFYQEYKTSPAIRMLVKTMAQKFGEEKGNSRYLQRLFPDGPAKQ
                     ATKIAGLPKPAKCL"
     gene            complement(6964..7629)
                     /locus_tag="PWX02_001201"
     CDS             complement(6964..7629)
                     /locus_tag="PWX02_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BAX inhibitor protein"
                     /protein_id="HDL1729220.1"
                     /translation="MENRLAHSYSTGESLISTHKVLRNTYMLLAMTLTFSAVVAFVAI
                     QMNAPAMPWWGLLVGFYGLLFLTNATSNSSAGILSVFALTGFLGYTLGPILNRYIGAG
                     LGDIVALAFGATALVFFACSAYVLTTKKDMSFISGMMVALFVVLLVGIIANIFLAIPA
                     LSLALSSLFVIFSSGAILLATSNIIHGGETNYIRATVDLYVSIYNLFVSFLQIFGVLG
                     SDD"
     gene            7835..8383
                     /gene="pgsA"
                     /locus_tag="PWX02_001202"
     CDS             7835..8383
                     /gene="pgsA"
                     /locus_tag="PWX02_001202"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDL1729221.1"
                     /translation="MKLNFPTYLTLFRVGLTPLFIVAFYLPIKYAPEISTLIFFIASI
                     TDAFDGYLARKWNQTTRLGAFLDPVADKVLVAVAFVCVAEYYHTWWITIPICIMIARE
                     IIISALREWMAELGERASVAVSIWGKVKTTAQMLALGGMLWRQNQLMEILAFILLYIA
                     VGLTIWSMMQYLNASKNSLLKS"
     gene            8461..10299
                     /gene="uvrC"
                     /locus_tag="PWX02_001203"
     CDS             8461..10299
                     /gene="uvrC"
                     /locus_tag="PWX02_001203"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDL1729222.1"
                     /translation="MSEFNAKSFLLNVPHQPGVYRMYDSKGTIIYVGKAKDLKKRLAS
                     YFRSQLSSKKTEALVANLANIETTITHSETEALLLEHNYIKENQPKYNVLLRDDKSYP
                     YILLTKHKHPRLTAFRGSKKIAGEYFGPYPNVTAVRETLNLLQKIFPIRQCEDTYYKN
                     RSRPCLQYQIGRCLAPCVEGCYSPVEYDQQVNLVRLFLQGKDHQVVEHLVQKMEDAAE
                     ALDFEGAARFRDQIQSVRAVQEKQFVSNDRLDDLDIISIVYQNGIACVHILFVRQGKV
                     LGNRSYFPKVPSNTDLTELADTFVGQFYLQMNQHRTIPNQIVIDHQLSESKALSEILS
                     EQAGHKVSILDNAIRGDKQRYLALARTNAMAALALQLKQESHIQSRYQALKEMLKMSE
                     IKRMECFDISHTMGNQTVASCVVFDENGPLKSDYRRFNIEGITGGDDYAAMEQALLKR
                     YNRELPPEKIPDIIFIDGGKGQLNRALDVFAKLDVEWDKSKPLLIGVAKGVERKAGLE
                     TLIISKWDKEIHLPPDSLALHLIQHIRDESHQHAISGHRKKRQKAFTESGLESIAGVG
                     AKRRQALLKYLGGMQGVKSATLEEIQSVPGISKALAEVIFDTLKNS"
     gene            complement(10304..10579)
                     /locus_tag="PWX02_001204"
     CDS             complement(10304..10579)
                     /locus_tag="PWX02_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729223.1"
                     /translation="MKKLILLGLSVALTACSTPQQQSTVPMDMQAVQDYHQRVINKQT
                     VDKNTKQHDEPLNQSDKRSESINLPIHPIIYPSIGVGYHYGWKRYRY"
     gene            complement(10704..11057)
                     /gene="rplT"
                     /locus_tag="PWX02_001205"
     CDS             complement(10704..11057)
                     /gene="rplT"
                     /locus_tag="PWX02_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009635878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDL1729224.1"
                     /translation="MARVKRGVIARARHKKVLKAAKGYYGARSRVYRVAFQAVVKAAQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGLSYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFAALVEKAKSAL"
     gene            complement(11166..11363)
                     /gene="rpmI"
                     /locus_tag="PWX02_001206"
     CDS             complement(11166..11363)
                     /gene="rpmI"
                     /locus_tag="PWX02_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDL1729225.1"
                     /translation="MPKIKTVRGAAKRFKKTASGGFKRKQSHLRHILTKKTTKRKRQL
                     RHKSMVAKADQVLVVACLPYA"
     gene            complement(11627..12169)
                     /gene="infC"
                     /locus_tag="PWX02_001207"
     CDS             complement(11627..12169)
                     /gene="infC"
                     /locus_tag="PWX02_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDL1729226.1"
                     /translation="MKPVKRVQSNRAHRLNDEIRGVKEVRLTDENGEQVGIVSLQDAL
                     NRAEEAELDLVEISPNAEPPVCRIMNYGKFIYEKEKAAKEQKKKQKVVQVKEIKFRPG
                     TDDGDYQVKLRSIIRFLEDGDKVKITVRFRGREMAHQEIGMDVLERVKADTAEIAMVE
                     SAPGKLEGRQVVMVIAPKKK"
     gene            complement(12328..>13123)
                     /locus_tag="PWX02_001208"
     CDS             complement(12328..>13123)
                     /locus_tag="PWX02_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HDL1729227.1"
                     /translation="DYAWLQKILLEEPESEQRNIKQHKLQAIGDFAESQTCRRLVLLN
                     YFGEHRQEQCKNCDICLDPPQQYDGLLDAQKVMSVIYRTGQIFGQHHIIAVLRGMNNL
                     KIREHQHDQLSVYGIGKEQSQHYWQNVVRQLIHLGLIIQNIANHSALQLTESARPFLR
                     GEKQLQLALPRLGISATAFVQKQASHRYDKDLFARLRFLRKQIADLENIPPYIVFNDA
                     TLQEMAEFLPTSKVEMLEINGVGERKFERFGQAFMSLIKEYQNNRK"
BASE COUNT         3805 a         2543 c         2888 g         3887 t
ORIGIN      
        1 tttcattata atatgagctg atttgataat aaagtttatc agataaacgc aataataaat
       61 gcgttttttc atattattca ctttaggagg aaatcaatat gtcttcattc caaaattgga
      121 cagaacacac agctcacgtt aagcaatctt ttggtaaatt agggcaaaaa caccctaaaa
      181 tgcttgaagc ttataaagca ttaggtacag cggccgcagc ggaagcatta gatcacaaaa
      241 ctcgtgaatt aattgcatta gcagtagcga ttacaacacg ttgtgaaagt tgcatcagtg
      301 tacacgcaga agaagcggca aaagcaggtg caaccgaaag cgaaattgcc ggtgcattag
      361 cgactgcgat tgcattaaat gccggtgcgg catataccta tgctttacgt gcattagaag
      421 cggtggaaga gcaaaaataa ggagcaataa atgaagaaat tggcttggat tttaggcttt
      481 gcagctatga tgccaatgat gtctagtgct aaacaagcat cagttcagca agtgcaggct
      541 gaaaaagcag cgggcatttg gattgatgta cgttctgctg aagaatttca gcaagggcat
      601 ttaaatggtg caattaatgt aacgcatact gaaattgcac aacgtatcgc agaagttgct
      661 ccagataaaa atcagccgat tcatctttat tgccgctcgg gtcatcgagc tgaagcggcg
      721 cttaatgagc taaaaaaatt aggttacagc aatgtaacta atcatggtgg ttatgacgat
      781 ctgattaaag ccgggttgaa ataactgttt tgagtgccaa gcatttgctt ggcacttttc
      841 gtttatatct gaggttaaaa attatgagtg cttttaacaa ttttgttaat tttgttgcaa
      901 atattgttgc tccaatgcaa cgccaattca ttaattttgt gcgtattgct atttgtattg
      961 tgatggtttg gattggtggt ttaaaagtct gccaatatga agcagacggt attgcccatt
     1021 ttgtatcgaa tagcccattc tttagttata tgtacaaaaa aggcccgaac ttagtcgatg
     1081 atggcacagg taaaatggtg atggaataca cccttcacaa aaatcctgaa ggcaaaatgg
     1141 tggcaaaaaa catcgaatgg cataaagaaa atggcactta taccgcttct tatatcatcg
     1201 gtgcaactat tgtgacagtt ggtttattaa ccttatccgg catttggttc ccagtttccg
     1261 gtatggctgg gggcttactc acttttggta tgtcgattgt tacactctcg tttatgatta
     1321 ccacccctga aatttgggtg ccgaatttag gtggggatat gccaacccct gcacacggtt
     1381 tcccatatct gtcggcagtc ggtcgtttaa ttgtaaaaga tgtgattatg atggcaggcg
     1441 gcttggtggc cgcagcggaa tgtgcaaatc gcattttagc cagacgtaaa gcgattgctt
     1501 aattaatagt gctaatgcaa gcggtcattt tcttgtaaaa ttttgcaata aaatgaccgc
     1561 ttgttttttg tcaatttgcc ataatttcaa gtataatcca attacgcaat cgtttgcttt
     1621 tatggagagg aataaaatga caacaatcgg tacaccgtta agaacgaacg ctacaaaagt
     1681 gatgatgtta ggttcgggcg agttggggaa agaagtggta atagagctgc aacgcttagg
     1741 cgtagaagtg attgcggtgg atcgttatga caatgcacct gcacagcaag tggctcaccg
     1801 tgcttatacg atttcgatgt tagacgggaa tgcattgcga gatttggtgg agaaagaaaa
     1861 accggatttt attgtgcccg aagtagaagc catcgcaact gcaaccttgg ttgagttaga
     1921 gcaagaggga tataacgtaa tcccaacggc aaaagcaacg caattaacaa tgaatcgaga
     1981 aggcattcgc cgattggcgg cagaagagtt gggcttaaaa acgtcgcctt atcgttttgt
     2041 ggataatttt gaacagttcc agcaagcgat tcaagaaatc ggcattccgt gtgtggtcaa
     2101 accgattatg tcttcttccg gacacgggca atcagtaatt aaatctgaag cggatattca
     2161 acaggcttgg gattattcgc aacagggcgg acgtgccggc ggtgggcgtg tgattgtaga
     2221 gggttttatc aaattcgatt atgaaattac ccagcttacc gttcgccata ttcacggcac
     2281 gtcgttttta gctcccatcg ggcatattca ggttgatggc gattatcgtg aatcttggca
     2341 accgcagcaa atgtcgggca ttgcattgaa aaaagctcaa gaaaccgcag aaaaaattac
     2401 ctctgcactg ggcggaagag ggattttcgg cgtggaactt tttgtctgtg gcgatgagat
     2461 tatttttaac gaagtctccc cacgtccaca cgataccgga atagtaacaa tggcatcaca
     2521 agagctttct caatttgctc tgcacgcccg tgcgattttg ggcttaccga ttccggaaat
     2581 ttaccgcatc agcccggcag cttctaaagc gattgtggtt gaagggaaat cggataatgt
     2641 gcgttttggc ggtgtagata aggtattagc cgaaatcggg acgaatatcc gcttattcgg
     2701 taaaggcgaa gtaaacggac accgccgatt aggggtaatt cttgctcgtg atgaaaatac
     2761 agtgagagca ttagaaaaag ctcggcgagc ttatgataag ttagatattc aattataaag
     2821 tcaggatttt gatattcgtt gatagtaggc attttatgcc tactatccta taggcctcta
     2881 atcaaattgg atgaaaaagt tgaagattgt taatcctctt tgccgagaat ttggttcact
     2941 tcgctcttat acaatactgc ttttgctcca aacacggctt gtacaccgcc accgacttcc
     3001 agcacgtcaa tcgcaccgag ttttttcagt gttgctttat ccacggcttt cacctctttc
     3061 actgccacac gcagacgggt aatacaagcg tccacatttt cgatattatt tgcagagcca
     3121 agagcggcga taattttatg ggcattttca gtcagtgagc tggtttgttg ttcgactacc
     3181 tcttcactct cttccgcacg tcccggtgtc attacgttga attttttgat caggaatagg
     3241 aacgagaagt agtaaagtgc cgcccacggc aagccgacta tgatcactcg aagccagtta
     3301 gtgttctcgt tgccttgcaa aatgccaaac agggtgaagt cgatcacgcc accggagaag
     3361 gtgttaccaa tcgagatgtt gagtaagtct gcaatgtaga acgacacgcc gtctaaaaaa
     3421 gcgtggaaca catagagcca cggggcaaca aacaggaaca taaattcaat cggctcggta
     3481 atgccggtga taaaagaggt gagcgctgca cctaagaaca gaccgctaat cgcaccacgg
     3541 cgggctttcg gcacagcgtg atacatcgct aaacaggcgg caggtaagcc aaacatcatc
     3601 gtatcgaaac gtccagcgaa gaaacgtgta ccttcggtaa ataagccttg atgggttgga
     3661 tcggcgagtt gggcgaagaa gattttttgc gccccgataa tggtttcacc atttaccact
     3721 tctgttccgc ccaattcggt gtaccaaaac agcgggtaga tcatatggtg taagccgacc
     3781 gcaccgctta accgcattaa gaaaccgtag aaaaaggtgc cgatttcgcc catttccgca
     3841 atcccttgcc ctgctgaaac cagccactgt tggaaagtcg gccagatcaa aaagaacact
     3901 gccccgacaa aaatcgcagc aaaggcggtc acaatcggca caaaccgaga gccgccaaag
     3961 aagccgagaa tttgcggcag ctgaatgttg tggtatttgt tatgcagttt caccgtaatc
     4021 aagcccatca ccagcgagcc gatcacaccg gtatcaatcc ctttggtttc aggagaaaac
     4081 agcggaatga gggcagaaat cgtgcctgtc ataatcaaat aaccgaccac tgctgccagt
     4141 gccgctacgc ctttatctcg ttttgctaat ccaatgccta aaccgataca cagtaataat
     4201 gcaaggttgc caaacaccac actgcctgcc gatgccataa tctggaaaat gccctgtaaa
     4261 gtcgggttat ccagtatcgg gtaggcttgc accgttgcct tattcgacaa agctccgcca
     4321 atccctaaca gcaaaccggc tgccggtaaa atcgcaatcg gcagcataaa agcttttccg
     4381 acttgttgca gttgtttgaa cataaaattt ctcctttttt atggttgtat ttgatggttt
     4441 atagcatact gaaatttagc cgtatgaggt gtgatataac tcacagattt gcaaaatttt
     4501 tagcaaattc gaccgcttgt aaaaaaatta ccgcttgcgg aaaaattgaa gtagaattaa
     4561 gcattttttt agcaaacaca acataaaaaa tttggagaag attatgccta ttttacgttc
     4621 agcaacttcc actcagggtc gtaatatggc gggggctcgt gcattatggc gtgcaaccgg
     4681 tatgaaagaa aatgattttg gtaagccgat tattgcagtg gtgaactcat tcacccaatt
     4741 tgtgccgggg cacgtccact taaaagatat ggggcagttg gttgcggcag aaattgaaaa
     4801 agccggtggg gtggcaaaag aattcaacac cattgcggtt gatgacggca ttgcaatggg
     4861 acacggcgga atgctttatt ctttaccaag ccgtgattta attgcagaca gtgtggaata
     4921 tatggtgaat gctcactgtg ctgatgcgat ggtgtgtatc tctaactgcg ataaaatcac
     4981 cccgggaatg ttaatggcag caatgcggtt aaatattccg actatttttg tctctggtgg
     5041 cccgatggaa gccggaaaaa ctaaactttc cgatcaatta attcgcttgg atttagtcga
     5101 tgcaatgatc gaaagtgccg acccgaatgt gtctgatgaa cgtattgatg cgattgaacg
     5161 tagtgcttgc ccgacttgtg gttcttgttc aggtatgttc accgcaaact caatgaactg
     5221 tttaactgaa gctttaggct taagtttacc gggcaatggt tcaatgttgg caactcatgc
     5281 tgaccgtaaa gagcttttcc taagagcagg tcgtgaaatt gtggaacttt gccgtaaata
     5341 ttaccaacaa gacgatgaga gcgtgttacc tcgctcaatc gctaccttcg atgcatttga
     5401 aaatgcaatg agccttgata ttgcaatggg tggttcaagt aataccgtgc ttcatttact
     5461 agcgactgct caagaagccg gcgttgattt taaaatggaa gatattgacc gcctctctcg
     5521 tgtcgtgccg tgcttaagta aaattgcccc gaataccaat aaataccata tggaagacgt
     5581 acaccgtgcc ggtgggatta tgggcttgct gggcgaatta gatcgtgcag gtttaatcca
     5641 tcgccacgtt aaaacagtgc taggtttaac gatagaagag caactgaatc aatacgatat
     5701 tattcgtaat aaagatgaag atttacacaa attcttccgt gcaggtccgg caggaattcg
     5761 tactactaag gctttctcgc aagattgccg ttgggacact gttgatgatg accgtgtaaa
     5821 cggctgtatt cgcaatgtgg aaaatgctgt gaccaaagag ggcgggttag cggtattatt
     5881 tgggaatatt gcagaagatg gatgtattgt taaaaccgca ggggtagatg agtctatttg
     5941 gaaattcact ggcaccgcaa tcgtgtttga aagccaagaa gatgcggtag caggtatttt
     6001 aggcggaaaa gtgaaagaag ggcatattgt gattatccgc tatgaaggtc cgaaaggcgg
     6061 cccgggtatg caagaaatgc tttatccaac tagttatttg aaatcaatgg gcttaggcaa
     6121 aaaatgtgct ttattaactg acggtcgttt ctctggcggt acctcagggt tatctatcgg
     6181 acacgcttca ccggaagccg cttcaggagg agcgattgcc ttaattaatg atggcgatac
     6241 ggtggcgatt gatattccga atcgttcgat taacttaatg gtttcagatg aagaacttgc
     6301 tgctcgccgt gcagaaatgg aagcccgtgg cgataaagcg tggcagccgc taaaacgtga
     6361 gcgagaagtg tcgtttgcac taaaagtgtt tggacatttt gccacctctg ctgataaagg
     6421 tgcggtgagg gataaatcgc tgttaaagta aataaattag aaaaaagaag catttcaata
     6481 atatattgat ctgctcttta ttcagctgaa tatttaatct agctgaatgg agagcagatc
     6541 gatagcctaa tttacatctt tttgttttga tacagggaaa tgtattacaa gcattttgcc
     6601 ggttttggca gacctgcaat tttagtggct tgttttgcag gaccatcagg aaacaagcgt
     6661 tgtaaataac ggctgttgcc tttttcttca ccgaattttt gtgccattgt ctttaccagc
     6721 attcgaattg ctggtgaagt tttgtattct tggtagaaat cccgcaccag ataaataatt
     6781 tcccaatgtt ctgcggttaa gctaatttgc tctttttcgg cgattttttc tgctaattct
     6841 acagaccaat catttaaatt agttaaatag cctgaagcat cggttgggta ttggatatta
     6901 tttaattcaa tcgtcatcaa ataaaaatgc cacataatgt ggcattcctc ttaaactaaa
     6961 acgctagtcg tcactaccta gcacaccgaa gatttgtaag aagcttacaa ataggttgta
     7021 gatagacaca tataaatcaa cggttgcacg aatgtagttg gtttcgccac cgtgaataat
     7081 gttgctggtt gccagtaaaa tcgcaccgct tgagaaaatg acaaacaagc tgcttagggc
     7141 taaacttaat gccggaattg ctaagaagat attggcaata ataccgacta ataataccac
     7201 aaacagggca accatcattc cgctgataaa gctcatatct tttttggtgg ttaatacata
     7261 agctgaacaa gcgaagaaaa ccaatgcggt tgcaccgaat gcaagggcga caatatcccc
     7321 taaacccgca ccgatgtaac ggtttaaaat aggacctaag gtatagccta agaagccggt
     7381 taaagcaaat acgcttaaga tacccgcact gctgtttgag gtggcattcg tcaagaaaag
     7441 taagccgtaa aagccgacca ataatcccca ccacggcata gccggtgcgt tcatttgaat
     7501 tgcaacaaaa gcaacaactg cggagaaagt cagggtcatt gccaataaca tataggtatt
     7561 gcgaagcact ttgtgggtgc taattagaga ttcaccagtt gaatagctgt gagctaaacg
     7621 attttccata aaaattcctt ctgtttttgt gtttaaatcg gaacatccga tggattaata
     7681 tctatggata aatttccata aatcaagaag taaggtagcc gtaatttatt gtaaagtcaa
     7741 tgcgattttg taacagaaga gtaaaaaaaa acatttagtt gtgttacact taataccact
     7801 ctactttata ttccttttca tcgatgaatt cgctatgaag ctcaatttcc caacttattt
     7861 aaccttattt cgggtaggat taaccccact ttttattgtg gctttttatc tgccgataaa
     7921 atatgcgcct gaaatttcaa cacttatttt ttttattgca tcgattaccg atgcctttga
     7981 cggctattta gcacgcaaat ggaatcaaac cacccgactt ggtgcatttt tagatcctgt
     8041 tgcggataaa gtcttggtag ctgttgcgtt tgtctgcgtg gcggagtatt accatacttg
     8101 gtggattact attccaatct gcattatgat tgcccgtgaa attattattt cggctttgcg
     8161 tgaatggatg gcggagcttg gggaaagggc cagtgttgcc gtttcgattt gggggaaagt
     8221 aaaaacgact gctcaaatgc tcgcattagg tggaatgtta tggagacaga accaactaat
     8281 ggagatttta gcctttattc tgctttatat tgcagtgggt ttaacgatct ggtctatgat
     8341 gcagtattta aatgcttcaa agaatagtct attgaagtca taagcggtca tatttttatt
     8401 cttttttgca atttgtagag tggaaatatc gctctacaat tttcaatttt atcaactcta
     8461 atgtctgaat ttaacgctaa atccttcctg ttaaatgtgc ctcaccaacc gggtgtttat
     8521 cgtatgtatg atagtaaagg cacgataatc tatgtgggta aagccaaaga tttaaaaaag
     8581 cgtttagcca gctattttcg tagccaactt tccagtaaaa aaacggaagc cttggtggca
     8641 aatcttgcta atattgaaac gacgatcact cattctgaaa cagaggcgtt actccttgaa
     8701 cataattata ttaaggaaaa tcagccgaaa tataatgtgt tgctcagaga tgacaaatct
     8761 tatccttata ttctgttaac taaacataag catcctcgtt taacagcatt tcgtggtagt
     8821 aaaaagatcg ccggtgaata ttttgggcct tatccgaatg ttacggcagt tcgggaaaca
     8881 ctgaatttat tgcaaaaaat ttttcctatt cgtcagtgtg aagatactta ttataaaaac
     8941 cgttcacgac cttgcttaca gtatcagatc ggacgctgtt tagccccttg tgtagagggc
     9001 tgttattcac ccgttgaata cgatcagcag gttaatttgg ttcgtttgtt tttacaggga
     9061 aaagatcacc aagtagtcga gcatctggta cagaaaatgg aagatgcggc ggaggctttg
     9121 gattttgaag gtgcggcacg ttttcgggat caaattcaat cagtaagagc ggtgcaagaa
     9181 aaacaattcg tctctaatga taggctagat gatcttgata ttatctcgat cgtttatcaa
     9241 aatgggattg cctgtgtgca tatcttattt gttaggcaag gtaaggtact aggcaatcgt
     9301 tcttactttc caaaagtgcc aagtaataca gatttaaccg agctcgcaga tacctttgtc
     9361 ggtcagtttt atttacaaat gaaccagcac cgcaccatac ctaaccaaat tgtaattgat
     9421 catcaattaa gtgaatctaa ggcactcagt gaaattttaa gtgagcaagc agggcataaa
     9481 gtcagcattt tagataatgc catcagaggt gataaacagc gttatttagc tttagcgaga
     9541 actaatgcaa tggcagcatt agctttacaa cttaagcaag aatcccatat tcaaagccgt
     9601 tatcaggcat tgaaagaaat gttgaaaatg tcagagatta aaagaatgga gtgctttgac
     9661 atcagtcata caatgggcaa ccaaacagtg gcttcttgcg tggtgtttga tgaaaatggg
     9721 ccgctaaaat cggattaccg ccgttttaac attgagggga ttaccggagg tgatgattat
     9781 gcagcaatgg agcaggctct cttgaaacgc tataatcgag aattaccgcc agaaaagatc
     9841 cctgatatta tttttattga tggtggcaaa gggcagttga accgagcgtt agatgtcttc
     9901 gctaaacttg atgtggaatg ggataaatcg aaaccgcttt taattggtgt ggcaaaaggc
     9961 gtagaacgta aagctgggct ggaaacttta attatcagca aatgggataa agaaattcac
    10021 ttaccgccag atagtcttgc cttgcattta attcagcata ttcgagatga atcccaccag
    10081 catgccatta gtgggcatcg taaaaaacgc caaaaggcct ttaccgaaag tggattagaa
    10141 tctattgccg gtgtcggtgc aaaacgccga caagcgttgc taaaatattt aggcggaatg
    10201 cagggggtga agtctgcaac cttggaagag attcaatcag ttccgggaat ttctaaagcg
    10261 ttggcagagg ttatttttga tacattaaaa aatagttaaa ttatcaatag cgatatcgct
    10321 tccagccgta atggtagccc actcctatgc ttgggtaaat tatagggtga attggcagat
    10381 ttatgctttc tgagcgttta tcgctttggt ttaatggctc atcatgttgc tttgtatttt
    10441 tatcaacagt ttgcttattg attactcgtt gatggtaatc ctgaactgct tgcatatcca
    10501 ttggtactgt gctttgttgt tggggtgtcg aacaggcggt tagtgcaacg ctcaatccga
    10561 gtaaaattaa ttttttcata tcatttcctt atattgcaaa atattttgct tttttgaccg
    10621 cttattttgc tattaattct taaaaagatt attttatcca taaaaaagga cgcactaggc
    10681 gtcctcattt ctattcttta aaattaaaga gcagattttg ctttttcaac taaagctgcg
    10741 aaagcaactt tgtcaaatac tgcgatgtca gctaagatct tacggtcgat ttcaacagat
    10801 gcttttttca agccgttgat gaatttgctg taagataaac cgttttgacg agctgctgcg
    10861 ttaatacgag caatccataa ttgacggaat tgacgtttac gttgacggcg gtcacggtaa
    10921 gcatattgag cagctttaac tactgcttgg aaagcaacgc gatacacgcg tgaacgtgca
    10981 ccataataac ctttagcagc cttaagaact ttcttatggc gtgctcttgc aataacacca
    11041 cgttttacac gagccattat ttaatctcct aattgtctat tttgattggt ggtcgtcatc
    11101 acgaccttta ctaagaattc tatccgtacg tccgtacttt actacactac gctaacctaa
    11161 aaggcttatg cgtatggtaa gcacgctact actaaaactt ggtcagcttt cgctaccatt
    11221 gatttgtgac gtaattgacg tttacgttta gtggttttct tagtcaaaat gtgacgtaag
    11281 tgagattgtt tacgtttaaa accgccggaa gctgtttttt tgaaacgctt agcagcacca
    11341 cgtactgttt taattttagg cattgttaaa aaactccgca ttgtttgtta gatattatga
    11401 aaataaggcg aattttgtga acaaaattac ttggaagcac ttattctccg ctatacgatt
    11461 ttcatcgtta aaaagtcaag caggctgctg agtttgcctg ttgagcattt tctctttctt
    11521 ggctaggcaa aaaagcttgc gaattttagc gaaaatataa ccgctatgca agccaattta
    11581 agctttatca agaaaaaagc acattaaaac aatgtgctta tcgaaattat ttcttcttag
    11641 gtgcgatcac catcacaact tggcgacctt ccaatttgcc tggggctgac tctaccattg
    11701 caatttctgc agtatctgct ttgacacgtt ctaaaacgtc cataccgatt tcttggtgag
    11761 ccatttcacg accacggaaa cgcactgtga tttttacttt atccccatct tctaagaaac
    11821 ggataatgct acgaagttta acttgataat cgccatcatc agttcccgga cggaatttga
    11881 tctctttaac ttgaacaact ttttgttttt tcttttgttc ttttgctgct ttctcttttt
    11941 cgtagatgaa cttaccatag ttcataatac gacaaactgg tggttcagca tttgggctga
    12001 tttccactaa atccaattca gcttcttctg ctctatttag agcatcttga agtgatacaa
    12061 taccaacttg ttcgccgttt tcatcggtta agcgaacttc ttttacgcca cgaatttcgt
    12121 cattaagacg atgtgcacga tttgattgaa cacgttttac aggtttaata gccttattcc
    12181 ttctagtaaa tttttgctta aattttaccg cttgagagcg tataaatatt ttagattgct
    12241 tgttttaagt gattaaaaaa cagctaaacg tgcggattct ataagatttt cagtgcttat
    12301 gcaataataa agcccttatt taactgccta ttttcggtta ttttgatatt ctttgattaa
    12361 actcataaat gcttgcccaa atcgttcaaa ttttcgttca ccaacaccat taatttctaa
    12421 catttccact tttgaagtcg ggagaaattc tgccatttct tgcaaggtgg catcgttaaa
    12481 gacgatataa ggtggaatat tttcgaggtc agctatctgc ttgcgtaaaa aacgtaagcg
    12541 tgcgaatagg tctttgtcat agcgatgact tgcttgtttt tgcacaaatg cagtcgcaga
    12601 tatacctaaa cgtggcaatg caagctgaag ttgcttttct ccacgcaaaa acggtctggc
    12661 actttcggtt aattgcagag cggaatggtt agcaatattt tgaataatca accctaaatg
    12721 aatcagttgc cgcaccacat tttgccaata atgttggctt tgttcttttc cgataccata
    12781 aacggaaagt tgatcgtgtt gatgttcacg aattttgaga ttgttcattc ctcgcaatac
    12841 cgcaataata tggtgctgac cgaaaatttg accggtgcgg taaatcaccg acatcacttt
    12901 ttgggcatca agtaagccat cgtattgttg cggtggatct aaacaaatat cgcagttttt
    12961 gcattgctct tggcgatgtt ccccaaagta gttgagtaag actaaacggc ggcaagtctg
    13021 tgattcggcg aaatcgccaa ttgcctgtaa tttatgctgt ttaatatttc tctgttcgct
    13081 ttccggctct tccaataata ttttttgtaa ccaagcataa tcg
//
LOCUS       DANJWC010000058        12945 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.058, whole genome shotgun sequence.
ACCESSION   DANJWC010000058 DANJWC010000000
VERSION     DANJWC010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12945
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.058"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(31..1227)
                     /locus_tag="PWX02_001209"
     CDS             complement(31..1227)
                     /locus_tag="PWX02_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL1729228.1"
                     /translation="MKQVIRFIVFSTLSLFSFSTFAKTIVAIDAGHGGKDPGAIGKNL
                     GILEKSVTLAISKELKALLDADPNFKAVMTRSGDYFIQLPQRTEIARKHRADLLVSIH
                     ADSSPRGDSLKGASVWVLSNSRASDEMGKWLEDHEKQSELLGGAGSVLSSNNERYLNQ
                     TVLDLQFSHSQRAGYELGKSILAKMSNVAALAKSSPQHASLSVLRSPDIPSVLVETGF
                     LSNSTEEAKLASANYRKQVARAIYNGLVAYRAKNAVASSSVSKKSDKEDKQNKKEEKT
                     AKADLTKKPASEKQEKNTQSDKTKLKEKSKKEDKKEDSAKTNKKKEQQLKEKTALEKD
                     QKNQSKKSDSQREIKVSNSGYHIVQQDETVYSIARAYGTTPQKISELNNLKNYQITVG
                     KKLKVK"
     gene            complement(1247..1738)
                     /gene="tsaE"
                     /locus_tag="PWX02_001210"
     CDS             complement(1247..1738)
                     /gene="tsaE"
                     /locus_tag="PWX02_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDL1729229.1"
                     /translation="MTDSLSFYCEDETKLLEFGQSFALELKKYLLQDQAHSLVVYLNG
                     ELGAGKTTLTRSIVRAFGHQGNVKSPTYTLVEEYQLPPFSLYHFDLYRLADPEELEFM
                     GIRDYFKPQTLCLLEWAVKGKGMIPEADFVIQIDYKNDGRQISLLPQNQTAVDILINF
                     HQK"
     gene            complement(1738..2292)
                     /gene="orn"
                     /locus_tag="PWX02_001211"
     CDS             complement(1738..2292)
                     /gene="orn"
                     /locus_tag="PWX02_001211"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HDL1729230.1"
                     /translation="MAQNEQNLIWIDLEMTGLDPEKERIIEIATIVTDKDLNILAEGP
                     VLAVHQTDDLLEKMSDWCVKTHTANGLIERVKASKLTERAAELQTLDFLKRWVPKGAS
                     PICGNSIAQDKRFLYKYMPDLADYFHYRHLDVSTLKELARRWKPEILDKFSKGNTHLA
                     LDDIRESINELKFYREHFIQLDQK"
     gene            complement(2302..2781)
                     /gene="coaD"
                     /locus_tag="PWX02_001212"
     CDS             complement(2302..2781)
                     /gene="coaD"
                     /locus_tag="PWX02_001212"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDL1729231.1"
                     /translation="MSYNVIYAGTFDPITNGHLDIICKASHLFGKVIVAVAQNPSKQP
                     LFSLEERVELVKQSCTQWQNIEVIGFSGLLADFAKQQNAKALIRGIRGADDIDYEIQL
                     AQLNYKLSGDLETIFFPPSIEWRYLSSTMVREIYRHNGDISQFVPQAVKNALKIAKV"
     gene            complement(2783..4063)
                     /gene="waaA"
                     /locus_tag="PWX02_001213"
     CDS             complement(2783..4063)
                     /gene="waaA"
                     /locus_tag="PWX02_001213"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HDL1729232.1"
                     /translation="MLRLLYICLSYLLQPVVLLLMWYKGRKQPAYRKRLWERYGIYDE
                     SEKPKAKGVVIHAASVGEVIAATPLIKAVSKQYPELPLIVTTVTPTGSGRVQAAFGNS
                     VSHFYLPYDLPDAIERFLNFIDPKLMIVIETELWPNLIRKVNIRKIPFVIANARLSPR
                     SAKRYGWVKGSIKDMLNQISLIMAQDEVSRDRYLALGYAPAKMVNTGNLKFDLEITPQ
                     LHQSVIKTKASLNLTERPIWIAGSTHEGEEKLILDAHKLLLEHYPNLVLILVPRHPER
                     FGLVELLIQKSDLNYVKRTENKTLSQNTQVMLGDTMGEMMLLYGLSDIAFVGGSLVKH
                     GGHNPLEPIAFNIPVVSGLYTYNFPEIFEKLREVKGVIEIESSVEALTESIQLLLKQP
                     QIGQEIAKSGLSVLQENQGALKRHLDLLASYLEK"
     gene            complement(4343..5722)
                     /gene="yegQ"
                     /locus_tag="PWX02_001214"
     CDS             complement(4343..5722)
                     /gene="yegQ"
                     /locus_tag="PWX02_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HDL1729233.1"
                     /translation="MTLKYAKPELLSPAGSLKNMRYAFAYGADAIYAGQPRYSLRVRN
                     NEFNHANLKVAIDEAHALGKKFYVVVNIAPHNSKLKTFIKDLKVVVDMKPDALIMSDP
                     GLIMLVRENFPEMDIHLSVQANAVNWATVKFWRQMGLTRVILSRELSLEEIEEIREQV
                     PDMELEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYKMEEGKIDEVGQI
                     VPKYEPEIEVKNVAPTLGNGEATDKVFLYTESQRPDEQMTAFEDEHGTYFMNSKDLRA
                     VQHVERLTQMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDESLMDTLESL
                     AHRGYTEGFLRRHTHDEYQNYEYGYSISERQQFVGEFTGKRNAEGLAEVAVKNKFLVG
                     DSVEMMTPKGNIVFKIERMLNRKNENVDAALGDGHFVFLDVPADIELDFALLMRNLVN
                     TNTRNPHQA"
     gene            5850..6221
                     /locus_tag="PWX02_001215"
     CDS             5850..6221
                     /locus_tag="PWX02_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729234.1"
                     /translation="MKLKYLLATILLAFSTFTVAQSIKTEMMQMNMQANKLMRAESAE
                     EFSEIANDFIIITEKTKGIFPNSVEGDKAAQEDYQAGMQKLIDMVNQAIEKAQAGELQ
                     EAKMLISELEMIKREYHKKYK"
     gene            complement(6260..9736)
                     /gene="dnaE"
                     /locus_tag="PWX02_001216"
     CDS             complement(6260..9736)
                     /gene="dnaE"
                     /locus_tag="PWX02_001216"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDL1729235.1"
                     /translation="MSDCRFVHLKVHSDFSMINGLAKVKSLVKTAVANNMVAMGLTDF
                     TNFCGLVKFYGEALGAGIKPIIGADVYVRTEAGSEDYFELTLLAKNNKGYHNITQILS
                     KAYQQGYIEFPMVEREWLAELNEGIIILSGGRMGDVGKALLKENRQEAEEKVAFYQQY
                     YPNHFYLSVCRTGRADEERYIKQAVEFSQKNAIPLVAVNDVVFLKEDDFDAHEIRVAI
                     HDSYTLDDPKRPKKYSPKQYFRTEEEMCKLFADLPSALANTVEIAKRCSVTVRLGEYF
                     LPNFPTGELSTEDFLVKKSKEGLEERLEFLFPDPEERANKRSVYDERLQVELDVINQM
                     GFPGYFLIVMEFIQWSKDNDIPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFLN
                     PERVSMPDFDVDFCMDGRDRVIEHVAETYGRQAVSQIITFGTMAAKAVIRDVGRVLGH
                     PYSFVDRISKLIPPDPGMTLEKAFAAEPKLPELYEADEEVKDLIDMARKLEGVTRNAG
                     KHAGGVVIAPTAITDFSPLYCDSEGLHPVTHFDKNDVEYAGLVKFDFLGLRTLTIIKW
                     ALEMINERLAREGKEPIRIETISLDDKKSFALLLAAKTTAVFQLESRGMKDLISRLKP
                     DCFEDIIALVALFRPGPLESGMVQNFIDRKHGKEEVSYPDPQYQHECLKPILEPTYGV
                     IVYQEQVMQIAQELAGYTLGGADLLRRAMGKKKPEEMAAQREVFEKGAEAKGIDGKLA
                     GQIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTSEMDNTDKIVG
                     FYDECMNMGLTVVPPDVNSGKHRFSVNEKGEIVYGLGAIKGVGEGPIEAILAARQKEG
                     KFKDLFDLTARVDLKKINRRTFESLIMSGAFDKLGPHRAALMKNLEDALKASDQHAKM
                     EELGQSDMFGVLTETPEEVQNAYANTPKWSEQVILDGERATLGLYLSGHPIGRFLKEL
                     SHYAPNRLNELQPTFRGQMTTVSGIVMASRVAVTKRGSRIGIATIEDRSGKLDITLFS
                     EALENFGHLMQQDQIIVATGSVQFDDFSGGLKMSAREVLSLDEARGRFAKSLALAISK
                     EQLSADFIKKMTAIITPHKEGTLPLHFYYQSPEGRVLLKGGVEWRISPNEAMFSELKA
                     LLGENSVELEFE"
     gene            complement(9962..11059)
                     /gene="lptG"
                     /locus_tag="PWX02_001217"
     CDS             complement(9962..11059)
                     /gene="lptG"
                     /locus_tag="PWX02_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDL1729236.1"
                     /translation="MQFFSMNILERYIGKTILSAIFLTLFMLIGLGAIIKFVEEFRDV
                     GRGSYDGLKAAYYTFLTIPRDIETFFPIAALLGSLLGLGNLASRSELVVMQASGFSRF
                     RIGLAVMKTALPLVLFTMIIGEWGVPQTEQFARNMRSIAQSGGSMLATKDGFWAKDGN
                     SFIYINHIKNEKSLDNVLIYQFGEGQNKRELQSVLKAGTANYSDEKGWVLSKVEKSTV
                     GENQIQQAKQPDQVWQTSLTPSKLGIVSLKPESLSISGLADYVGFLKDTGQDPKRFEI
                     TFWRKVFQPISMAVMMLLAISFIFGPLRSSTMGAKIVIGIVAGFMFYVANIVFGNLSL
                     IATWLPVPVGALIPSLLCLIIVWWLLSKKRD"
     gene            complement(11059..12168)
                     /gene="lptF"
                     /locus_tag="PWX02_001218"
     CDS             complement(11059..12168)
                     /gene="lptF"
                     /locus_tag="PWX02_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDL1729237.1"
                     /translation="MILSRYLTKEIFKSQLAILFILLLIFFCQQLVRVLSSAVSGKVP
                     ADLVVSLLGLGMPTMAQLMLPLSLFIALLLTLGRFYAESEITVMRACGIGQGLLVKVA
                     MFLSIFTTALAVYNVFWLTPWAINKQSEMLAEAKANPRFAALSAGQFMSAGGYVLFID
                     KIENNSLNDIYVFQPEQQKKNKPSVVVAESGTLQGLPNGDQVLTLAESKRFEGTPQSA
                     DFRISHFENYSAYLGYQDVESDEKLVQRADFKKLMETKTPEAKAELHWRLALILAVPL
                     MALLAVPMSSVNPRQGRFAKLLPALLLYLIYFLLQSSLKSSGASGKLNAEVLMPLVSV
                     AFLVLGVILNAWDSKFMSKLRYQFGAKKLTNKAVA"
     gene            complement(12342..>12945)
                     /locus_tag="PWX02_001219"
     CDS             complement(12342..>12945)
                     /locus_tag="PWX02_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729238.1"
                     /translation="DITRKISLQNETETAIKTINEQIESQLTNIDVRFEKIGERLFLV
                     VSAKDVAPLLQNEPKEVQFVGDVIFDGEPNLTEQAQSTAEATKVQNILNIATNQEKIM
                     AAKGKKAPQYKTVTKDNIGLKNLRVTALGTFLVFDTSVDAAKLRVKGNFEDIAQGENI
                     IAILHQNTEAPPKIEIEDSQGKKLILEAHKATSSKKSKKQ"
BASE COUNT         3690 a         2989 c         2407 g         3859 t
ORIGIN      
        1 gctgatggat atttccatca gcctttcaat ttacttcacc ttcaattttt tacctaccgt
       61 gatctgataa tttttcaaat tattcagctc actgattttt tgcggcgtag taccgtaagc
      121 tctagcaatc gagtaaaccg tttcatcttg ttggacaata tggtagccac tgttagacac
      181 cttaatttct ctttgagagt ctgatttttt agactgattt ttttgatctt tctcaagagc
      241 tgttttttct tttagttgct gctctttttt cttattggtt ttagccgaat cttctttttt
      301 atcctctttt ttgctctttt ctttcaattt tgtcttatct gattgcgtat tcttctcttg
      361 tttctcagaa gcaggctttt tagttaaatc agctttagca gttttttcct ctttcttatt
      421 ttgcttatct tctttgtccg attttttact gaccgaagaa ctagcaactg cattttttgc
      481 ccgataagcg actaatccat tataaatggc ccgagcaact tgtttacgat aatttgcact
      541 ggctagcttt gcttcctcgg ttgaattaga taaaaatccc gtttccacca atacagatgg
      601 aatatcaggt gaacgcagaa cacttaagct tgcgtgctga ggagacgatt ttgctaatgc
      661 agccacattt gacattttag ctaaaatgct cttgcctaat tcatagccgg cacgttgaga
      721 gtgagagaac tgtaaatcaa gcactgtttg atttaaataa cgttcattgt tgcttgaaag
      781 caccgaacca gccccgccaa gtaattccga ctgcttctcg tggtcttcca accatttacc
      841 catttcatcg ctagcacggc tattagaaag tacccaaacc gaagcccctt tcaaggaatc
      901 gccacgaggc gaagagtctg cgtgaataga aaccagcaga tcggcacgat gcttacgggc
      961 aatctcggta cgttgcggta attggataaa ataatctccc gagcgtgtca tcactgcttt
     1021 aaaattagga tcggcatcta acaaggcttt tagctcttta gaaattgcga gggtaacgct
     1081 tttttccaaa atccccagat ttttaccaat agcccccgga tcttttcccc cgtgtccggc
     1141 atcaatcgcc acaatggttt tggcaaatgt ggaaaaactg aataaactca atgtgctaaa
     1201 aacaataaat ctgataactt gcttcattac cttccctaca agcggtctat ttttgatgaa
     1261 aatttattaa tatatctact gcagtttgat tttgcggcaa caggctaatc tgccgtccgt
     1321 cattcttata atcaatttgg atcacaaaat cagcctctgg aatcattcct ttacctttaa
     1381 ctgcccattc aagcaaacaa agcgtttgtg gtttgaaata atcacgaatt cccataaact
     1441 ccagctcttc aggatcggct aagcgataca aatcaaaatg gtaaaggctg aaaggcggaa
     1501 gttggtattc ttccaccaga gtataagttg ggctttttac attgccttga tgcccgaaag
     1561 cccgtacgat cgagcgagtt aaggtggttt tacctgcccc aagttcgccg tttaggtaaa
     1621 caaccagcga atgagcttgg tcttgcaata aatatttctt aagttctaac gcgaatgatt
     1681 gaccaaattc cagcaattta gtttcatctt cgcagtaaaa agagagagaa tctgtcatta
     1741 tttttggtct agctgaataa aatgttcccg ataaaatttc aattcgttaa tggattcacg
     1801 aatatcgtcc aacgctaaat gcgtattgcc tttactgaat ttatctaaaa tctccggttt
     1861 ccaacggcgt gccagctctt ttaaagtact cacatctaag tggcgataat ggaaataatc
     1921 ggctaaatca ggcatatatt tatacaaaaa acgtttatct tgagcaatgc tgttgccaca
     1981 aatcggagaa gcgccttttg gcacccagcg ttttaagaag tctaaagttt gcaactctgc
     2041 tgcccgttca gtcaatttgc tcgctttcac tcgttcaatc agcccgttag cggtatgggt
     2101 tttgacgcac caatcactca ttttctctaa taaatcatcg gtttggtgta ccgcaagtac
     2161 cggtccttcc gctaaaatat ttaaatcctt atcggtcaca atggtggcga tttcaataat
     2221 gcgttctttt tccggatcta accctgtcat ttcaagatcg atccaaatta aattttgttc
     2281 gttttgagcc attttatttc cttacacttt tgcaattttt agggcatttt tgaccgcttg
     2341 cggcacaaat tggctgatgt caccattgtg gcgatagatt tcccttacca tcgttgaaga
     2401 aaggtaacgc cattctattg aaggggggaa aaaaatagtt tctaaatcgc ccgataattt
     2461 gtaatttaac tgagcgagtt ggatttcata gtcaatatca tctgccccac gaataccacg
     2521 gattaacgct ttggcattct gctgtttggc aaagtctgct aacaatccac taaagccaat
     2581 cacctcaata ttttgccact gggtacagct ttgtttaaca agctccactc gttcttcaag
     2641 actaaaaagc ggctgcttgc ttgggttttg agcaacggca acaatcactt ttccaaataa
     2701 gtgagaggct ttgcaaatta tgtccaaatg cccattggta atgggatcaa aggtgcctgc
     2761 ataaattacg ttataactca ttttatttct ctaaatagga ggctaataaa tccaaatggc
     2821 gttttaatgc cccttgattt tcctgtaaca cgcttaagcc ggattttgca atttcttgtc
     2881 cgatttgggg ctgcttaagc aataattgaa tgctctcggt caaagcctcg actgaacttt
     2941 caatttcaat cacgcctttc acctcacgca atttctcaaa gatttcaggg aaattatagg
     3001 tgtaaagccc agaaaccacc ggaatattaa aagcgattgg ttcaagtgga ttatgcccac
     3061 cgtgtttgac caaactgcct cctacaaaag caatgtcaga caacccatac agtaacatca
     3121 tttcccccat tgtatcacct aacattactt gagtattttg gcttaacgtt ttattctcag
     3181 tacgttttac ataatttaaa tctgattttt gaatcagtag ctcaactaaa ccaaaacgct
     3241 ccggatggcg aggcactaaa atcaacacta aattcgggta atgctctaat aagagtttat
     3301 gagcatctaa aatcagcttc tcttcccctt cgtgagtact gccggcaatc caaatcggtc
     3361 tctctgttaa attcagacta gctttagttt taatcaccga ttgatgaagc tgtggcgtaa
     3421 tttctaaatc aaatttcagg ttaccggtat taaccatttt tgccggtgca taaccgagtg
     3481 ctaaatagcg atctcggctt acctcatctt gagccataat cagactaatt tggttgagca
     3541 tatccttaat gctacctttt acccagccat aacgcttcgc agaacgtggc gataagcggg
     3601 cattggcaat cacaaatgga attttacgaa tatttacttt acgaattaaa ttcggccaaa
     3661 gttcggtttc aattacaatc atcaatttcg gatcgataaa attcaaaaac cgctcgatag
     3721 catcaggtaa gtcataaggc aaataaaaat gtgaaacact attaccaaaa gcagcttgca
     3781 cacgcccaga gcctgttggc gttaccgttg tgactatcaa cggaagttca ggatattgct
     3841 tgctgactgc cttaatcaaa ggcgttgcag caatcacctc accaaccgat gccgcatgga
     3901 ttaccacccc ttttgctttc ggtttttccg attcgtcata aatcccataa cgctcccata
     3961 aacgtttccg ataagccggt tgtttacgcc ctttgtacca cattaatagc agaaccacag
     4021 gctgtaatag ataacttaaa caaatataga gtaaacgtag catttatttt ccttatacca
     4081 taaccctcgc ctcttgtggg agagggggaa tattgcaaac tgttacaaaa aaacgaccgc
     4141 ttgccctctc tctgataaaa ttgctgaaca caattttatc ggtctctctc ccacaagggg
     4201 agagagtcaa atacgccaat aaatcgttcc tctcttgttc gagagggggg aacaagcggt
     4261 tatttttacc aaaaattttg caatctatat cctcaaagac aaaaccacgg aataacctta
     4321 atccgtggtt tatctcgcat cattacgctt gatgtggatt gcgggtattc gtattcacta
     4381 agttacgcat taaaagtgca aaatccaact caatatctgc cggcacatct aaaaagacaa
     4441 aatgcccgtc accgagtgct gcgtccacat tttcattttt gcggttaagc atacgctcga
     4501 ttttaaatac aatgttgcct ttaggggtca tcatttccac tgaatcgccc actaagaatt
     4561 tgtttttcac tgccacttcc gccaagcctt ctgcattgcg tttgccggta aattcgccca
     4621 caaattgttg gcgttctgaa atcgaatagc catattcata gttttggtat tcatcgtgag
     4681 tatgacgacg taagaagccc tctgtataac cacggtgtgc aaggctttca agagtatcca
     4741 ttaaactttc gtcaaacggt ttaccggcag cggcatcatc aatcgcctta cgatacacct
     4801 gagccgttct cgcacagtaa tagaacgatt tcgtacgccc ctcaattttc agtgaatgca
     4861 cccccatttg agttaaacgc tcaacgtgtt gtactgcacg cagatcttta gagttcataa
     4921 aataggtgcc gtgttcatct tcaaaggcgg tcatttgctc atcagggcgt tgtgattctg
     4981 tataaaggaa cactttatct gttgcttcgc cattacctag agtaggagcc acatttttga
     5041 cttcaatttc cggctcatac ttaggcacaa tttgccctac ctcatcaatc ttaccttctt
     5101 ccattttata ttcccaacgg caagcattgg tgcaagtgcc ttggtttggg tcacgcttat
     5161 tgatatagcc tgaaagcaag cagcgacctg aatatgccat acacaaagca ccgtgaacga
     5221 agatctcaag ctccatatcc ggcacttgtt cacgaatttc ttcgatctct tcgagtgata
     5281 attcacggga taaaatcaca cgggttagcc ccatttgacg ccagaatttc accgttgccc
     5341 agtttaccgc attggcttgc accgaaagat gaatatccat ttcagggaaa ttttcacgca
     5401 ccaacataat taagcccgga tcagacataa tcaaagcgtc cggcttcata tccactacca
     5461 cttttagatc tttgatgaag gtttttaatt ttgagttgtg tggcgcaatg tttaccacca
     5521 catagaattt ttttcctaag gcgtgggctt catcaatcgc aacttttaaa ttggcgtggt
     5581 taaattcatt attacgcaca cgcaagctat aacggggctg tcctgcataa atcgcatcgg
     5641 caccataagc aaaagcgtaa cgcatatttt ttaaagaacc tgcaggcgaa agtagctcag
     5701 gtttggcata ttttaaagtc atattattct cttttctgat aacaagtcag gacttctcga
     5761 cgtgagaagc gtaagcggta tattttctaa gaaatattgc gtgatggcaa gctaatccat
     5821 tacaatccga tttttatgtg gggaaaaata tgaaacttaa atacctttta gcaacaatat
     5881 tactggcttt ttcaacattt accgtcgctc aaagcatcaa gacggagatg atgcaaatga
     5941 atatgcaagc gaataaattg atgcgtgccg aatcggcaga agagtttagc gaaattgcga
     6001 atgatttcat tataattacc gagaaaacca aagggatttt tcccaatagc gtagaaggcg
     6061 ataaagcagc tcaggaagat taccaagccg gtatgcaaaa attaattgat atggttaatc
     6121 aagccattga gaaagctcaa gcaggtgaat tgcaagaagc aaaaatgctg atttccgagc
     6181 ttgaaatgat caagcgtgaa tatcacaaaa aatacaaata attcaacaca ttaaaagggt
     6241 gagctaaagc tcaccctttc tattcaaatt ccagttcaac cgaattctca ccaagaagtg
     6301 cctttaattc gctaaacatt gcctcattcg ggctgattcg ccactctact ccgcctttga
     6361 gcaataccct accttccggg ctttggtaat aaaaatgcag cggtaaagtg ccttctttat
     6421 gtggggtgat gatagcggtc atttttttaa taaaatctgc agaaagttgc tctttgctaa
     6481 tcgctaacgc taaacttttg gcaaatcgac cccttgcttc atcaagggat aacacttctc
     6541 gagccgacat tttcagtcca ccgctgaaat catcaaactg taccgaacct gttgccacaa
     6601 taatttggtc ttgttgcatc aaatgcccga aattttctaa ggcttccgag aaaagggtaa
     6661 tgtccaattt ccccgagcga tcttcaatag tcgcaatccc aatacggctt cctcgcttgg
     6721 taactgccac tcgagatgcc ataacaattc ctgaaaccgt ggtcatttgc cctcggaaag
     6781 tcggttgcaa ttcattcagc cgattagggg catagtggga taattctttg aggaatctac
     6841 caatcgggtg tccgcttaaa tacagcccga gcgtggcacg ctctccatct aaaatcactt
     6901 gctccgacca ttttggcgta ttggcgtaag cattttgcac ttcttccggt gtctcggtaa
     6961 gcacgccaaa catatcgctc tgcccgagtt cctccatttt agcgtgctga tctgaggctt
     7021 taagtgcatc ttccaaattc ttcatcaagg cagctcggtg agggcctaat ttgtcaaaag
     7081 caccggacat aatcaggctt tcaaaggtgc ggcggttgat ttttttcaga tcgacccgag
     7141 cggttaaatc aaatagatct ttaaatttcc cctctttttg gcgagctgcc agaattgcct
     7201 caatcggtcc ttccccaacg cctttaattg ctcctagccc gtaaacaatt tcgccttttt
     7261 catttacgct aaaacgatgt ttaccgctgt ttacatcagg cggcacaacg gttaagccca
     7321 tattcataca ctcatcgtag aacccgacaa ttttatcggt attatccatc tctgaggtca
     7381 ttaccgcagc cataaattct gccggataat gggcttttag ccaaagtgtt tggtaagaaa
     7441 ccaacgcata agctgctgag tgagatttat taaagccgta gccggcaaac ttttctacca
     7501 agtcaaaaat ttgaccggca agtttaccgt caatcccttt ggcttccgcc cctttttcaa
     7561 acacttcacg ctgtgcagcc atttcctccg gtttcttctt acccattgca cggcggagta
     7621 agtccgctcc accgagtgta taaccggcaa gctcttgtgc aatttgcata acctgttctt
     7681 ggtaaacaat gaccccataa gtcggctcga gaataggttt taagcattcg tgttggtatt
     7741 gcggatcagg ataagaaacc tcttctttgc cgtgtttacg gtcaataaag ttctgcacca
     7801 tacctgattc cagcggccct gggcggaata gtgccaccaa tgcgataata tcttcaaaac
     7861 agtccggctt caggcgagaa attaagtctt tcattccccg tgattcaagc tggaatactg
     7921 ctgtagtttt agctgccagc aacagagcaa aggatttttt atcatcaagc gaaatggttt
     7981 caatccgaat cggctctttg ccttcacgag ccaaacgttc gttaatcatc tccaacgccc
     8041 atttgatgat ggtgagcgta cgcaagccta agaagtcaaa cttcaccaat cctgcatatt
     8101 ccacgtcatt tttatcaaaa tgggtaaccg ggtgtaagcc ctcgctatcg caataaagcg
     8161 gcgaaaaatc ggtaatcgca gttggggcga tcaccacacc accagcgtgt ttacctgcat
     8221 tacgagttac accttcgagc ttacgagcca tatcaataag gtctttcacc tcttcatcgg
     8281 cttcatatag ctcaggcaat tttggctcag cggcaaaggc tttttcaagc gtcattcctg
     8341 gatcgggcgg aatgagtttg gagatgcgat ccacaaagct ataggggtgt ccaagcactc
     8401 gccctacatc tcgaatgacc gctttcgccg ccatagtacc gaatgtgata atttgcgata
     8461 ccgcttgtcg cccgtaggtt tccgcaacgt gttcaatcac acggtctcgc ccgtccatac
     8521 agaaatcaac gtcaaaatcg ggcattgaaa cacgctcagg gttgagaaaa cgctcaaaaa
     8581 gcaaatcaaa ttccaatggg tcaagatcgg taatcttcaa cgcataagcc accaatgagc
     8641 ctgcccctga gccacgaccc ggtcctaccg gaatatcatt atctttggac cattggataa
     8701 actccatcac gatcaaaaag taacccggga accccatttg gttgatcaca tctaattcaa
     8761 cttgcaatct ttcgtcataa accgaccgct tgttcgccct ttcctcagga tcagggaata
     8821 gaaactctaa acgctcttct aagccttctt tcgatttttt taccaagaaa tcttcggtgg
     8881 aaagctcacc ggtcgggaaa tttggcagga aatactcgcc caaacgcacg gtaaccgaac
     8941 agcgtttcgc aatttcaacc gtgtttgcca atgcactcgg taaatcggca aacagcttgc
     9001 acatttcctc ttcagtacgg aaatattgtt tcggtgagta ttttttcggg cgtttcgggt
     9061 cgtctagggt gtagctgtcg tgaatggcaa cccgaatttc gtgtgcgtca aagtcatctt
     9121 ctttcaagaa gacaacatca tttaccgcca ctaacggaat tgcatttttt tgtgaaaatt
     9181 cgaccgcttg tttgatatag cgttcttcgt ctgcccgtcc ggtacggcaa acggaaaggt
     9241 aaaaatgatt cgggtaatat tgctgataaa aagccacttt ttcttcagct tcttgtcggt
     9301 tttctttgag taaggcttta cccacatcgc ccattcgccc gcccgaaaga atgataatgc
     9361 cttcattcaa ctctgccagc cattcacgtt ccaccatcgg aaattcaata tagccttgct
     9421 gataggcctt agacaaaatt tgggtaatgt tatggtagcc tttattgttc tttgcaagta
     9481 atgtcagttc aaaataatct tcactgcctg cctcggttct cacataaaca tccgccccga
     9541 taatcggctt aattccagct cccaatgctt cgccataaaa tttcaccaac ccgcaaaaat
     9601 tagtaaaatc cgtcaagccc atcgccacca tattattggc aaccgccgtt ttgactaacg
     9661 atttaacctt agctaaacca ttaatcattg agaaatcact gtgaactttt aaatgaacga
     9721 aacgacaatc tgacatagga atataatcta agtaaagcag ttcccctctt tagtaaagac
     9781 tgtcaggttc taaaaagtga agcctgctgt gaataaaatc ccgttgggcg aatgttattc
     9841 gcccctacaa acgagcatta ttcttgctca gagttacatt taaaatccga atgtaggggc
     9901 gaattacatt cgccctttca gaacctgaca accctgcttt atctaaagag gggagtaaaa
     9961 attaatcacg ttttttgctt aacagccacc aaacgatgat caaacagagt aggcttggaa
    10021 ttaatgctcc aaccggtaca ggtaaccaag tggcaattaa gcttaaattg ccaaacacaa
    10081 tatttgccac atagaacata aagccggcaa caataccaat cacaattttc gcccccatcg
    10141 tactactacg aagcggcccg aaaataaagg agatcgcaag tagcatcatc accgccattg
    10201 agattggttg gaatactttt cgccagaagg tgatctcaaa acgttttgga tcttgtccgg
    10261 tatcttttaa gaagccgaca taatctgcca agcctgaaat cgagagcgac tccggtttga
    10321 gtgaaacaat gcctaatttg ctaggggtta aactggtttg ccaaacttga tccggctgtt
    10381 tagcttgctg gatctgattt tctcccacag tcgatttttc cactttagac aacacccagc
    10441 ctttttcatc gctgtaattc gcagtaccgg cttttaaaac ggattgcaat tcacgtttgt
    10501 tttgtccttc gccgaattga taaatcagta cgttatctaa gcttttttca tttttaatat
    10561 gattgatata gataaatgaa ttgccatctt tcgcccaaaa cccgtctttt gtcgcaagca
    10621 ttgagccacc gctttgagca atcgaacgca tattacgggc aaattgctcg gtttgtggca
    10681 cgccccattc gccaataatc atagtaaaaa gtaccagcgg taaggcagtt ttcatcacgg
    10741 ctaaaccaat gcggaaacgg gaaaaaccgg aggcttgcat caccaccaat tcactgcggc
    10801 tcgcaaggtt gcctaaaccg agtaatgagc caagtaatgc cgcaatcggg aaaaaggttt
    10861 cgatatctcg tggaatagtt aaaaaagtat agtatgccgc ttttaagcca tcgtaagagc
    10921 cacgccctac atcacgaaac tcttccacaa atttaataat cgccccaagc ccaatcaaca
    10981 taaacagggt taaaaaaatc gcggataaaa tcgttttgcc gatgtagcgt tctaaaatat
    11041 tcattgagaa aaattgcatt atgccaccgc cttattggtt aatttttttg caccaaattg
    11101 atagcgtaac ttagacataa atttactatc ccacgcattt aaaataacac ctaaaactaa
    11161 aaaggcaact gataccaacg gcattaacac ttccgcattc aacttgcctg atgcccctga
    11221 cgatttcagc gagctttgta gtaagaaata gattaaataa agcaataacg ccggcagtag
    11281 tttagcaaaa cgcccttgac gagggtttac actactcatt ggcaccgcta ataatgccat
    11341 taacggtaca gctaaaatca aagcaagccg ccaatgaagc tcggcttttg cttccggcgt
    11401 tttggtttcc attaatttct tgaaatcggc tcgttgcact aatttctcat cagattcaac
    11461 gtcttgatag cctaaataag cagaataatt ctcaaaatgt gaaatacgga aatcggcact
    11521 ttgtggtgta ccttcaaaac gcttgctctc tgccaaggtt aagacttgat caccatttgg
    11581 caaaccttgc aacgtgccgg attcagcaac aaccactgaa ggtttgtttt tcttctgctg
    11641 ttccggctgg aaaacgtaga tatcatttag actgttattt tcaattttat caataaataa
    11701 cacatagcca ccggcagaca taaattgtcc ggccgaaaga gccgcaaaac gtgggttggc
    11761 tttcgcttcc gctaacattt cactctgttt attaattgcc caaggcgtaa gccaaaacac
    11821 gttataaacc gctaatgcag tggtaaaaat cgagagaaac atcgccactt taaccaatag
    11881 cccttgcccg ataccacaag cccgcattac ggtaatttca ctttcggcat aaaaccgccc
    11941 gagggttaat aacaaggcga taaacagtga gagtggcagc attaattgtg ccattgtcgg
    12001 catacctaag cctaataagc tcaccactaa atctgccggt actttaccac tcacagcaga
    12061 acttagaaca cgcactaatt gctgacaaaa aaagatcagt aataagataa acaatatcgc
    12121 caattggctt ttaaatatct ctttggttaa atatcggctt aagatcacgt tttttctcta
    12181 taacatttac aaatctgaat accgctaaac acaaacggtc tatttttgtt aattttttgc
    12241 aaagagcgca aaataactta ttcgaatcgc taataatacc tttttcatac aaaaaaatca
    12301 cctatagaaa atatcacgca cggcaaaagc gtgcgtaagt attattgttt tttcgatttt
    12361 ttcgatgatg ttgctttatg agcttcaaga attaatttct taccttggct atcttcaatt
    12421 tcaatcttag gtggtgcttc cgtattttga tgaaggatag ctataatatt ttcaccttgt
    12481 gcaatatctt caaagtttcc tttcacacgt aacttagccg catcaacact agtgtcaaat
    12541 actaagaatg tacctaatgc ggtaactcta agatttttaa gccctatatt gtccttcgtt
    12601 accgttttat attgaggtgc ttttttccct tttgcagcca taatcttctc ttgattagtc
    12661 gcaatattaa gaatattttg aactttagtc gcttctgctg tagattgtgc ctgttctgtt
    12721 aaatttggtt ctccatcaaa aataacatcc ccaacaaatt gcacctcttt aggctcattt
    12781 tgcaataatg gtgcaacgtc tttagctgaa acaaccaaaa ataaccgttc acctattttt
    12841 tcaaaacgga catcaatatt ggttaattgt gattcaattt gttcattaat tgtcttgatc
    12901 gcagtttctg tctcattttg aagcgatatt ttacgtgtaa tatca
//
LOCUS       DANJWC010000059        12927 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.059, whole genome shotgun sequence.
ACCESSION   DANJWC010000059 DANJWC010000000
VERSION     DANJWC010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12927
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.059"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..1330)
                     /locus_tag="PWX02_001220"
     CDS             complement(<1..1330)
                     /locus_tag="PWX02_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL1729239.1"
                     /translation="MKGSGLATVGAPETGDNGQTIYTVDVAKAGAPTVTRGNVAVKEG
                     DENKVMTAGDVADAINNSEKTSSVVAGSKAVTVKAGKEDDKGNTEYTVDVATDKSIGR
                     DNDGNMTVNTDNVTIVKDPTTGEVKANTTTLNNSPEGKVTEPTGDDAKKLVTAGDIAN
                     AINNSGFNVTAGDGVDGGETKGKKTQLIKPSETVTFDAGKNMTLTQADGKFTYTTKDN
                     VAFNSIDMSNGKPDTTGSITNLKSGVGGTFADKAAPTDAERKAIADNINNATGDTLNN
                     AVNVGDVQAATTKVEGDKGVTVTSKTNDDGSTTYTVEAKTDGTTIKVNDKGEITANTS
                     ELTNNPDGKVEEPTEPNALVTAKTVADAINNAGFNIQANGDEKSLVKTGDTVQFLNGK
                     NIEITRDGNITVSTAKNVNFDSVQFGNEGPKITNNGGNINVGDKDGNAVKV"
     gene            complement(1293..1778)
                     /locus_tag="PWX02_001221"
     CDS             complement(1293..1778)
                     /locus_tag="PWX02_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729240.1"
                     /translation="MDTGSITVDNTTGAVTTPAEEDKVATTKTVSEAIQKAGWNAKSG
                     GNKADSDQEAAELINPGEEVIFAAGDNLKVKRVGTTFTYETAKDVKFDSVTFGDNGPK
                     ITNKDGNVNIAGNDGNPTKITGVKAGEADTDAVNVSQLKQAAASQNRSERFRFSNCWC
                     T"
     gene            complement(1851..3971)
                     /locus_tag="PWX02_001222"
     CDS             complement(1851..3971)
                     /locus_tag="PWX02_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL1729241.1"
                     /translation="MGARATSANAIALGTDTAATGNRATAFGAGALATGNRSTVMGWR
                     SAASGTRSFAMGSGATANADFSGAMGDKSVANATYSLALGSSANATSTATRGTAIGYN
                     ATVSGVNATALGVSAVSSNQSTVAIGDSAKASAGFAIALGSGANAALNNSVALGSNST
                     VSNAVAVPSATVNGITYSGFAANTTAAGNVVSVGSNTIKRQIQNVAAGQNSATSTDAI
                     NGSQLYMAMNATGNLANSTKNILGGNATVNPDGSVTYTNIGGTNKNTIEEALKAVKTE
                     VVAGSNVNITNATGANGQTIYTVNAYNTTASSSSPDYITVTGKAATAANTTNYEIGLT
                     KKAIDDFTKDTQATVVSNDGTVTVKSTERNANGTVIYDLSVNIPAQASQIQYFSVNST
                     VPENQANDGAKSRNSIAIGPNATATGGEQAAVALGTNSNANGNGALSLGVVTVSKGIQ
                     ATAVGHSANATANGTTALGRQTNATAGDATAVGSNANATAEKASAFGVAANASANASL
                     AVGANSIASAQSAVAVGTRANATAQFATALGMGAQATLNSSVALGSESVVRAATPTEN
                     ATVGGITYNGFAGVNKDTNYVVSVGSAGKERQIQNVAAGQISATSTDAINDSQLYMAM
                     NATSNLANSTANNFGGGSVVNPDGSVTQPKYNVTNAAGTQYGNTATNVGDAITNLNNY
                     VNQGFNIKDNAGETKGTVTPNESV"
     gene            complement(4200..4386)
                     /locus_tag="PWX02_001223"
                     /pseudo
     CDS             complement(4200..4386)
                     /locus_tag="PWX02_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250451.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR domain-containing protein"
     gene            4750..5904
                     /locus_tag="PWX02_001224"
     CDS             4750..5904
                     /locus_tag="PWX02_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HDL1729242.1"
                     /translation="MNQFFPLIFSLTLFSGVTLALEKAPRTMPTEGINKPLLGQILFF
                     DNMLSHNGSQNCSSCHNPDYAFIDNRTTPAKGVVSQGDTPTKFGKRNSPTMLYAKYSP
                     EFHFDEQSQEYIGGQFWDGRAKNLAEQAGMPPIDPNEMGMPNKLEVAKRLYNVPMYQR
                     FLGQFYGEKVWESYESVYAAMEDAIATFQQENKLLAPFDSKYDLFLKGEVQLTALEEK
                     GRALFFNKAQTNCSSCHQLQSAANHQEETFSNYHYYNVGVPGNPDLIAVNGFAPDFVD
                     LGLFENPNVKGDETQKGKFKTPTLRNVAVTAPYMHNGVFKELKTVLLFLDSYNNPERK
                     INPETGTAWQQAEFEPTIAHQKLKAKPLSDEEIDALKAFLKTLTDERYRK"
     gene            complement(5952..6941)
                     /locus_tag="PWX02_001225"
     CDS             complement(5952..6941)
                     /locus_tag="PWX02_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HDL1729243.1"
                     /translation="MNDLIIYNTEDGKSNVALLVVENEAWLTQNQLAELFDTSVQNIS
                     EHIKNILKDKELDENSVIKDYLITAQDGKNYQVKHYSLEMILAIGFRVRSPRAVQFRK
                     WANSTLRTYLEKGFLIDSERLKNPQGRLDYFDELLEQIREIRASELRFYQKVRELFKL
                     SSDYDKTDKNTQMFFAETQNKLIYAITKHTAAELICQRADATKMNMGLTSWKGKVVRK
                     GDIVIAKNYLDNNELDSLNRLVMIFLESAELRVKNNQDLTLGFWRRNVDMLIEFNGFS
                     VLGNGGTITHKQMESFVREQYEKFDIQRKCLKQKEADWEDLQALEKLESELTR"
     gene            complement(7101..8465)
                     /gene="mpl"
                     /locus_tag="PWX02_001226"
     CDS             complement(7101..8465)
                     /gene="mpl"
                     /locus_tag="PWX02_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HDL1729244.1"
                     /translation="MKKHIHILGICGTFMGGVAMIARELGYKVTGSDTNVYPPMSTFL
                     AAHGIEIIPNYDVAQLQPAPDLVVIGNAMSRGNPCVEYVLDNQLNYTSGPQWLHDHLL
                     KDKWVLAVSGTHGKTTTTGMLAWILDQNGIDTGFLIGGVAGNFGISARAGNSPFFVIE
                     ADEYDSAFFDKRSKFVHYTPKTLIINNMEFDHADIFDDLKAIQRQFHHLVRTMPQSGC
                     ILSAIADQNVQDTLKMGSYSQLQFIGEQNEWFAKPLNADCSQFEVFHLGVKQGEVHWS
                     IIGKHNMHNALMAIAAAHHAGVEVQGACEALGKFVNANRRLEVKGEVNGITVYDDFAH
                     HPTAIEATIDALRGKVGKNQRILAVLEPRSNTMKMGVHKDEIAPSLHDAEAVFVYQPD
                     IIAWSVSEITNVLSQPAYWSANIDDLVDLVVKEAKPTEHILVMSNGAFGGIHQKLLAK
                     LADK"
     gene            complement(8761..9516)
                     /locus_tag="PWX02_001227"
     CDS             complement(8761..9516)
                     /locus_tag="PWX02_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HDL1729245.1"
                     /translation="MKLKSFKPMFMLLSVAFLTACSSSINPETGVRDDPLEGFNRAMW
                     KVNYDYLDPYVLKPVAKGWKEYVPSPVKTGLINVANNLDEPVSFVNRLIEGEGKKAMV
                     HFNRFWINTVFGLGGLIDWASLDEGLRLNNGQRGLGESLGAHGVPSGSYIMMPAYGAT
                     TPRQGIGSAVETGYSVLTYVGSPWSIAKFVVQGVDTRSKLLEQDALLEQAQDPYVTFR
                     EAYFQNLEFKVKDGNVEQSQQEQLSEEELKNID"
     gene            9671..9988
                     /gene="metJ"
                     /locus_tag="PWX02_001228"
     CDS             9671..9988
                     /gene="metJ"
                     /locus_tag="PWX02_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HDL1729246.1"
                     /translation="MADWNGEYISPYAEHGKKSEQVKKVTVSIPIKVLEILTNERTRR
                     QIKNLRHATNSELLCEAFLHAFTGQPLPNDEDLMKDRADEIPEAAKQKMCELGIDPDE
                     WQY"
     gene            10036..10266
                     /locus_tag="PWX02_001229"
     CDS             10036..10266
                     /locus_tag="PWX02_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF997 family protein"
                     /protein_id="HDL1729247.1"
                     /translation="MYQQLTTEARWAVLLTLIYLLGWVGFAYFSPQGRGIFGFPIWFE
                     LSCIFLPVLFSLVATIVVKKVYKNIDLEVNNE"
     gene            10259..11683
                     /gene="panF"
                     /locus_tag="PWX02_001230"
     CDS             10259..11683
                     /gene="panF"
                     /locus_tag="PWX02_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HDL1729248.1"
                     /translation="MNSEMILPLIAYLIFVFGVAFYAYRKRQGGSFISEYYIGGRSMS
                     GFVLAMTTAATYVGASSFIGGPGAAYKYGLGWVLLAMIQVPAVLLSLGALGKKFALLA
                     RKHNSVTINDMLLARYQNKFVVWIAGLALLLSFFAMMTVQFIGAGRLLETTLGIPYQT
                     AVMIFAITVGLYTFIGGFRAVVLTDTIQGLVMIVGTSILLAGVIYAAGGVENAMHTLE
                     AINPQLTTPYGIDERPLDFTFMTSFWVLVCFGLIGLPHIAVRSMACKNSQALHRAMII
                     GTVVIAILMFGMHLSGVLGRAVVPDLKVPDQVIPTLMVQVLPPVVAGIFLAAPMAAIM
                     SSIDSMLIQSSSTLIKDLYLAIKPNAINNEKKIKLFSTITTLTFTVLLVFAALTPPEM
                     LIWLNLLSLGGLEATFLWVIVLGLYWEKANATGAIYSMLAGLSCYVLMTTFKIAIFSF
                     HAIVPSLLIGLIAFLIGNRFAPKV"
     gene            complement(11760..>12927)
                     /gene="nhaA"
                     /locus_tag="PWX02_001231"
     CDS             complement(11760..>12927)
                     /gene="nhaA"
                     /locus_tag="PWX02_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HDL1729249.1"
                     /translation="KSEASGGVLLLVFAFAAIVCANTSLSDFYFEFLQTIVSVQFGEF
                     ALNKPLLLWVNDGLMAVFFTLVGLEVKKELFEGSLSSYKKAIFPAIAAVGGMIVPAII
                     YWAINQNHPEYHNGWAIPMATDIAFAVGIVALLGTRVPLALKAFLLALAIIDDLGAIV
                     VIAIFYSQEISVQALTIAAIAIIGLVLLNRFKVTSLCAYLVVGLVLWTSVLKSGVHAT
                     LAGVIIGFCVPLKGKHGERPAEELEHILAPWCAYMILPIFAFANAGVYLLEMGMEQLS
                     SPLPLGIALGLIIGKTLGVFGFSYLAVLFRLASLPQGVNFKQIFAIAVLCGIGFTMSM
                     FLASLAFDVSEAGESITALARLGILLGSAVSAILGYTLLRMATKQNNPENNATY"
BASE COUNT         3647 a         2916 c         2443 g         3921 t
ORIGIN      
        1 ttactttgac tgcattaccg tctttgtcac caacattgat attaccacca ttgttagtaa
       61 tcttaggacc ttcatttccg aactgtacac tatcaaaatt cacatttttt gcagttgaaa
      121 cagtaatgtt cccatcacgg gtaatctcaa tgtttttacc atttaagaac tgaaccgtat
      181 cacctgtttt caccaggctc ttctcgtcac cgtttgcttg aatgttaaaa cctgcattgt
      241 taattgcatc tgcaacggtt ttagctgtta ctaatgcatt tggttctgta ggctcttcca
      301 ctttaccatc agggttattt gttaactcgc tggtgtttgc cgtgatttcg cccttatcgt
      361 ttaccttaat ggttgtacca tccgtttttg cttcaacggt ataagtggtt gagccgtcat
      421 cgttagtttt agatgttaca gtaacacctt tatcaccttc cactttggtg gttgctgctt
      481 gtacatcacc cacattcaca gcattattca acgtatcacc tgttgcgttg ttaatattat
      541 ccgcaatcgc tttacgctct gcatctgttg gtgctgcttt atctgcaaat gtaccaccca
      601 cgccgctctt cagattagtg attgagcctg ttgtatctgg tttaccattg ctcatatcaa
      661 tgctgttgaa tgcaacatta tctttcgttg tgtaagtgaa tttaccgtca gcttgggtaa
      721 gtgtcatatt tttacccgca tcaaaggtaa ctgtttcact tggcttgatt agctgtgttt
      781 tcttaccttt ggtttcacca ccatcaacac catcacctgc tgttacatta aagcctgaat
      841 tattgattgc attagcaata tcacctgccg tcactaattt cttagcatcg tcacctgtag
      901 gctcagtaac tttaccttca ggagaattat ttaatgttgt ggtattcgct ttaacttcgc
      961 ctgttgtcgg atctttcacg atagtaacgt tgtcagtatt cactgtcata ttaccgtcat
     1021 tatcacgacc gatagattta tccgttgcaa catctaccgt atattcagta ttgcctttat
     1081 catcttcctt tccggcttta acagttacgg ctttagaacc agctactaca gatgaggttt
     1141 tctctgaatt attaattgca tcagccacat cacctgcggt cataactttg ttttcatcgc
     1201 cttctttaac agccacatta ccacgagtta ctgtaggagc acctgctttc gctacatcta
     1261 ccgtatagat tgtttgacca ttatcacctg tttcaggtgc accaacagtt gctaaacctg
     1321 aacctttcac ttcggttttg tgacgctgct gcttgtttta actgacttac attcaccgca
     1381 tcagtatcag cttcacctgc tttcacgcct gtaattttgg ttggattacc atcattacct
     1441 gcaatattaa cattaccatc tttattcgtg attttcgggc cattatcacc aaatgttacg
     1501 ctatcaaatt tcacatcttt cgctgtttcg tacgtgaatg tagtgccaac acgtttaact
     1561 tttaagttat cgcctgctgc aaaaatcact tcttcgcccg gattaatcaa ctctgctgct
     1621 tcttgatcac tatcagcttt attgccacca gattttgcat tccaacctgc tttttgaatc
     1681 gcttcagaaa ctgtttttgt agttgcaact ttatcttctt ctgcaggtgt agttactgca
     1741 cctgttgtgt tatcaaccgt gatagaaccg gtatccacat caaaggttac tttagttacg
     1801 ccatcagctt cttgtgtaac atttgaaact gtacctttac cgtttacaaa ttatacagat
     1861 tcatttgggg tcactgtgcc tttggtttca cctgcattat ctttaatatt gaaaccttgg
     1921 tttacatagt tatttaagtt cgtaattgca tcgccaacat ttgttgcagt attaccatac
     1981 tgagttccag ctgcattcgt tacattatat ttaggctgag tcacagagcc atccggattc
     2041 acaacagaac cgccaccaaa attatttgca gttgaatttg caaggttact ggttgcattc
     2101 attgccatat aaagttgtga atcattaatc gcatcggttg atgtagcaga aatctgaccc
     2161 gctgccacat tttggatttg acgctcttta cctgcagagc ctacagaaac aacatagtta
     2221 gtatctttat ttaccccggc aaagccatta taagtaatac cccctaccgt tgcattttca
     2281 gttggtgttg ctgctcttac gactgattcg ctacccaaag caacagaact attcagagta
     2341 gcttgagctc ccatacctaa tgctgtagca aattgagctg ttgcattagc acgtgtacca
     2401 acagccactg ctgattgtgc tgaggcaatt gaatttgcac ctacggctaa agaggcattt
     2461 gcactcgcat ttgctgcaac accaaaggca gaagcttttt cagccgttgc attagcatta
     2521 gaacctaccg ctgtagcatc acctgctgtc gcatttgttt ggcgaccaag tgcggttgta
     2581 ccattagccg ttgcatttgc tgaatgacca actgccgtag cttgaatacc ttttgaaacc
     2641 gtaacaacac ctaaagataa cgctccgtta ccatttgcat ttgagtttgt gcctaatgct
     2701 actgccgctt gctctccacc tgttgccgtt gcattcgggc cgatcgcaat tgagtttctg
     2761 ctcttcgcgc catcattagc ttgattctca ggtacggttg aattcacaga gaaatattga
     2821 atttggcttg cttgcgcagg aatattgaca gaaagatcat aaataaccgt gccatttgca
     2881 tttctctctg tactttttac agttactgta ccatcgtttg aaacaacagt agcttgggta
     2941 tctttcgtaa agtcatcaat ggctttctta gttaaaccaa tttcatagtt agttgtattc
     3001 gcagcagttg cagctttacc tgttacagta atgtaatcag ggctagaaga acttgccgtg
     3061 gtgttatacg catttactgt ataaatagtt tgtccatttg caccggttgc atttgtaata
     3121 ttaacattgc tacctgcaac aacttctgtt tttaccgctt ttagtgcttc ttcaatcgta
     3181 tttttatttg ttccaccaat attagtgtag gtaacagaac catcaggatt tactgtagca
     3241 ttaccaccta aaatattttt agttgaatta gcaaggttac ctgttgcgtt cattgccata
     3301 taaagctgtg aaccattaat tgcatcggtt gatgtagcag agttctgacc tgccgctacg
     3361 ttttggattt ggcgtttgat cgtgtttgag ccaacagaaa caacattccc tgctgccgta
     3421 gtattagccg caaagccgga ataagtaata ccatttaccg tcgcagaagg aactgccaca
     3481 gcgttggaaa cggttgaatt actccctaaa gcaaccgaat tatttagcgc tgcattagca
     3541 cctgagccta aggcaatagc aaaaccggca cttgctttag cactatcacc aattgcaacc
     3601 gtagattggt tggaagatac tgcacttacc cctaaagctg tcgcattaac acctgaaact
     3661 gtcgcattat aaccaatcgc tgtacctcta gttgcagtag aagttgcatt agcacttgaa
     3721 cctaaagcta aagaataagt agcatttgca acagacttat cccccattgc acctgaaaaa
     3781 tcagcatttg cggttgcacc tgaacccatt gcaaatgaac gagtgccact agcggcagat
     3841 ctccatccca ttactgttga acggttacca gtagctagag ctcctgcacc aaaagctgtt
     3901 gcacggttac ctgttgcagc agtatccgta cctaatgcaa tagcatttgc tgaagtcgct
     3961 ctagccccca taccaatagc aatagaagga actaatgccc aattctgacc agtattattg
     4021 agactctcta caccaatatt atagtaatgt aagaagtatc ttctggatcc actaaatttg
     4081 ttctatgcgt tgcattcgca tctgtaccaa ttgcaatagt agatctgcca gttgagattg
     4141 aatccgcacc aatagcaatt gcgttctccg atgatgcagt tgcttgtgtt gaaatataat
     4201 tacttgttgt tgaacctata gatacaacag ccacagcatc attagctccc aacacggcac
     4261 tagctccagc caataaagcc aatgttgcct tacttgcatc gttttacctt tcgctcgttg
     4321 taattcagat actgcaaccc aactttgtgt tgcctgattc caaataactt taaaaatttg
     4381 attcataatc gaccctatat aaaaataaaa aagattacgt agaacctatg ttcaacataa
     4441 tcttcaccaa tataaaaaac cgccacctat ttaagtgaaa taacttactt atagactcag
     4501 aaatttaagt gcgcgtattt tacgctattt gtaatataat tcatcttctg gagtgtaaat
     4561 ttattgtaaa taatacgagg aatctaaccg cttgttataa cgcaaaacac cttgacttaa
     4621 atcaagccac tacctcattt tgaggggaga tttatacgca gtgaaatttt aaaataagct
     4681 aaggtttacg gataatcgtt caacactatt gaattatcac tgtagttatc gttaaaaagg
     4741 tatgctttta tgaaccaatt cttccctcta atctttagct taactctatt ttccggcgta
     4801 actttggcac tagaaaaagc acctcgaact atgccgacag aaggaataaa taagccccta
     4861 cttggacaaa ttctattctt tgacaatatg ctgtcacaca acggttcaca aaactgctcc
     4921 agctgccata atccggatta tgcctttatt gataaccgca ctactccggc aaaaggggtg
     4981 gtttcacaag gagatactcc aaccaaattc ggcaaacgca actccccgac catgctttat
     5041 gcgaaatact cgcctgagtt ccactttgat gagcaaagtc aagaatatat cggcgggcag
     5101 ttctgggacg gacgagccaa aaatctcgcc gagcaagccg gtatgccgcc tatcgaccca
     5161 aatgaaatgg gaatgcctaa caaattagaa gtagccaaaa gattatataa tgtgccgatg
     5221 tatcagcgat ttttagggca attttacggt gaaaaggtat gggaaagtta tgaaagcgtt
     5281 tatgcggcaa tggaagatgc gattgcgaca ttccaacaag aaaacaaact tctcgctcca
     5341 tttgattcaa aatatgatct atttttaaaa ggcgaggtac aattaactgc attggaagaa
     5401 aaaggcagag ctttattttt caataaagct caaactaatt gtagtagttg ccaccaatta
     5461 cagagtgctg caaaccacca agaagaaacc tttagcaact atcactatta caatgtaggc
     5521 gtacccggta acccagactt gatagcggtt aacggcttcg caccagactt cgtcgattta
     5581 ggcttatttg aaaacccaaa tgttaaaggt gatgaaacac aaaaaggcaa attcaaaacc
     5641 ccaacattgc gtaatgttgc cgtgactgca ccttatatgc acaacggtgt gtttaaagaa
     5701 ttaaagactg ttttgctatt tttagacagc tacaataacc cggagcgaaa aatcaaccct
     5761 gaaaccggca cggcgtggca acaagcagaa tttgaaccca ctatcgccca ccaaaaactc
     5821 aaagccaaac ccttaagcga tgaagaaatt gatgccctca aagcattttt aaaaaccttg
     5881 acggatgagc ggtatagaaa ataaggctac aagcggtaaa attttgggga aattttaccg
     5941 cttgcgttta actaccgtgt tagctcactt tctaactttt ccaatgcctg caaatcttcc
     6001 caatctgcct ctttctgttt taaacattta cgctgaatat caaatttttc gtattgctct
     6061 ctgacaaagc tttccatttg tttatgcgtg attgttccac catttcctaa tacagaaaag
     6121 ccgttaaact caatcaacat atctacattt cttcgccaga aacctaacgt aagatcttgg
     6181 ttgttcttca cacgtaattc ggcactttca agaaagatca ttaccaagcg atttaaagaa
     6241 tccagctcat tattatctaa atagtttttc gcaatcacaa tatcgccttt acgcacgact
     6301 tttcctttcc agctagttaa tcccatattc atcttagtcg catcagcacg ttgacaaatc
     6361 aactcagcgg cagtgtgttt ggttattgca taaatgagct tattttgcgt ttctgcaaaa
     6421 aacatttgcg tatttttatc tgttttgtcg taatcgctag agagtttgaa aagctctcgt
     6481 actttttgat agaaacgcaa ttcacttgcc cgaatctcac gaatctgttc aagtaattca
     6541 tcgaaataat ctaaacgacc ctgcggattt ttcaaacgct cgctatcaat taaaaaacct
     6601 ttttcgagat aagtgcgtaa tgtgctgttt gcccattttc taaactgtac tgctcttggg
     6661 ctacgaactc gaaaccctat cgccagaatc atttcaagtg aataatgttt tacttgataa
     6721 ttcttgccat cttgagcagt tatcaagtaa tccttgataa ctgaattctc atctaactct
     6781 ttgtctttta agatattttt aatgtgttcg gatatatttt gcacagaggt gtcaaaaagt
     6841 tctgcaagtt gattttgtgt cagccaagct tcattttcaa ccactaacaa ggctacattg
     6901 cttttgccgt cttccgtatt ataaataatt aaatcgttca tattttattc tgtttattgg
     6961 tagagatatt agtcgctatt ttcaataaaa catgtataaa tatccagaat tgattttgaa
     7021 tttttcgatg cagatcacaa aaatagagag ttatacaaac aagcggtaaa attttgtaaa
     7081 aaatttaccg cttgtaaagg ttacttatca gctaatttgg caagtagttt ttgatgaata
     7141 ccgccaaagg ctccgttgct catcactaag atgtgctcgg tgggtttggc ttctttgacg
     7201 actagatcga ctaaatcatc aatatttgcc gaccaatacg ccggttgcga taacacattg
     7261 gtaatctcgc tcacactcca cgcaataata tcaggctggt aaacaaacac tgcttcggca
     7321 tcgtgtaggc ttggggctat ttcatcttta tggacaccca ttttcattgt atttgaacga
     7381 ggctctaaca ctgccaaaat gcgttgattt ttgccgactt taccacgcag agcatcaatg
     7441 gtcgcttcta tcgcagttgg gtggtgagcg aaatcatcat aaacggtaat gccgtttacc
     7501 tcgcctttca cttccaaacg gcggttagca ttcacaaact tgcctaatgc ctcgcaagcc
     7561 ccttgcactt caacccctgc gtgatgagcg gcggcaatcg ccatcagagc attatgcata
     7621 ttgtgcttgc cgataatcga ccaatgcacc tcgccttgtt taacgcctaa gtgaaacacc
     7681 tcaaactgtg agcaatcggc atttaacggt ttagcaaacc attcgttctg ttcgccaata
     7741 aattgaagct ggctgtaact gcccattttt agtgtatctt gcacgttttg atcagcgatt
     7801 gccgacaaaa tacagccact ttgtggcatc gtgcgaacaa ggtggtggaa ctggcgttga
     7861 atcgctttta aatcatcaaa aatatcggcg tggtcaaatt ccatattatt gataatcagc
     7921 gttttcgggg tgtaatgcac aaatttagaa cgcttatcaa agaacgcaga atcgtactcg
     7981 tccgcctcaa tcacgaagaa tggactgtta ccggcacgag ccgaaatgcc gaaattaccg
     8041 gcaacaccgc caattaaaaa gccggtatca atcccatttt ggtctaaaat ccacgctaac
     8101 attcctgttg tggtagtttt gccgtgcgtg ccggaaaccg ccagcaccca tttgtcttta
     8161 agcaaatgat cgtgcaacca ttgcgggccg gaggtgtaat tgagttggtt gtccaacaca
     8221 tattccacgc acggattacc acggctcatc gcattaccaa tcaccactaa atcaggagcc
     8281 ggttgcagtt gagcaacatc gtaattcggg ataatttcaa taccgtgagc agctaaaaag
     8341 gtactcatcg gtgggtaaac attagtgtct gagccggtca ctttatagcc aagctcacgg
     8401 gcaatcattg ccacaccgcc cataaaggtg ccgcaaatgc ctaaaatatg gatatgtttt
     8461 ttcatctttt tatatttaaa atgttagaat catcccctct ccccttgtgg gagagggata
     8521 gattttttct tcgtgcagaa gaaaaaatca gggagagggg taagcggtca tttttaggct
     8581 taatattgta agctccctct ctctgaaaaa tatttctgaa cgaaatattt ttcggtctca
     8641 atactcgcta cgctcgctcc tgtggagccg ttggcaaagc caacgttcaa atgtaaacat
     8701 ttgtcccaca aggggagagg tgcgctttag atgaggcgta ccatccctcg ttcacatcaa
     8761 ttaatcaata ttcttcaact cttcttccga aagctgctct tgttggcttt gctcaacatt
     8821 gccatctttc actttaaatt caaggttttg gaaataagct tcacggaagg taacataagg
     8881 gtcttgagct tgctctaaca acgcatcttg ttctaataat ttagaacggg tatctacccc
     8941 ttgtaccaca aatttagcga tagaccaagg tgacccaaca taagtcagca ccgaataacc
     9001 ggtttccacc gcactaccaa taccttgacg aggtgtggtt gctccataag ccggcatcat
     9061 aatataggag ccggaaggca cgccgtgagc gcctaaactc tcacctaaac cacgttgtcc
     9121 gttatttaaa cgtaacccct catctaaact cgcccagtca attaacccac ctaaaccaaa
     9181 cacagtgttg atccagaagc ggttgaagtg aaccatcgct tttttacctt ctccttcaat
     9241 taagcggttt acaaagctca ccggctcatc aaggttatta gcaacattaa ttaagcctgt
     9301 tttaaccggg cttggtacat actctttcca acctttagct accggtttta acacataagg
     9361 gtctaaatag tcgtaattga ctttccacat cgcacggtta aacccctcaa gtgggtcgtc
     9421 tcgcacgccg gtttctgggt taatgcttga tgaacaagcg gttaaaaatg ccacactgag
     9481 tagcataaac atcggtttga acgacttgag tttcataaat tgaacctttt gcagagaaat
     9541 ttgtccattg tagcgaagtt gataaaaaat acaaaacaaa aagccgtttt gcctctagaa
     9601 atctagacgt ctagattgag atatagttcg cttttgatta caatacaggc aacatttaag
     9661 gagcaaacaa atggcagact ggaacggcga atatattagc ccttatgccg aacacggcaa
     9721 aaaaagtgaa caagtcaaaa aagtgacggt atccattcca attaaagtat tggaaatttt
     9781 aactaatgag cgaacccgtc gccaaatcaa aaatcttcgt cacgcaacca acagcgaatt
     9841 attgtgcgag gcttttttgc acgcctttac cggtcagcct ttgccgaatg atgaagactt
     9901 gatgaaagac agggcagatg aaatccctga agcggcaaaa caaaaaatgt gtgagctggg
     9961 aatagacccg gacgaatggc aatattagac acaccttgca actgcgaggt tctttttatg
    10021 ttttaaggag actttatgta tcaacaatta acaacagaag cacgttgggc tgtgttgctt
    10081 acgctgattt atctgctcgg ctgggtaggt tttgcttact tttcaccgca aggcagaggc
    10141 attttcggct ttccgatttg gtttgaactt tcttgcattt tcttacccgt actatttagc
    10201 ttagttgcca ccattgtcgt caaaaaagtg tataaaaata tcgatcttga ggtgaataat
    10261 gaatagtgaa atgattttac ccctgattgc ttatttaatt tttgtgtttg gcgtagcgtt
    10321 ttatgcttac cgtaaacgcc aaggcggaag ctttatttcg gaatactata tcggtgggcg
    10381 ttcaatgtcg ggctttgtgt tagcaatgac aaccgccgcc acttatgtgg gggcaagttc
    10441 ctttatcggt gggccgggtg ctgcctataa atacggttta ggctgggtgt tactggcaat
    10501 gattcaagtg cccgcagtgc tactctctct tggagcatta ggcaaaaaat ttgccttact
    10561 tgcccgcaag cataatagtg tcaccattaa cgatatgtta ttagcccgtt accaaaacaa
    10621 atttgtggtt tggattgcag gactagcctt attactttcg ttttttgcga tgatgacggt
    10681 gcaatttatc ggtgccggtc gcttacttga aaccacactc ggaatccctt accaaacagc
    10741 ggtaatgatt tttgccatca cagtcgggct ttataccttt atcggcggtt ttagagcggt
    10801 ggtgctaacc gatactattc aaggtttagt aatgatagtc ggcacaagca ttttactcgc
    10861 tggtgtgatt tatgccgcag gcggagtgga aaatgcaatg cacacccttg aagccattaa
    10921 cccacaactt accacccctt acggcattga cgaacgcccg cttgatttca cctttatgac
    10981 ttcattttgg gtattggttt gctttggttt aatcggctta ccgcatattg cggtcagaag
    11041 tatggcatgc aaaaacagcc aagccttgca ccgagcaatg attatcggca cggtagtcat
    11101 tgcaattcta atgtttggaa tgcacctttc aggcgtatta ggcagagcgg ttgtgcctga
    11161 tctcaaagtg ccggatcaag tcattccaac actaatggta caagtgctac caccggtggt
    11221 tgccggtatt ttccttgccg ccccaatggc agcgattatg tcttcgattg actcaatgtt
    11281 gattcagtcg tcttcaacct taattaaaga tctctatctg gcaattaagc cgaatgccat
    11341 taataacgag aaaaaaatca aattgttctc cactattact actcttactt ttacggtatt
    11401 actcgtgttt gccgccctaa ccccgcctga gatgttaatt tggctgaacc tactctcttt
    11461 aggtggttta gaagcgactt tcctatgggt aattgtacta gggttgtatt gggaaaaggc
    11521 aaatgccacc ggtgctattt actcaatgct ggcaggctta agttgttatg tattaatgac
    11581 tacctttaag attgctattt ttagcttcca cgccattgtg ccttcactgc taattggttt
    11641 gattgctttc ttaatcggca accgatttgc tcctaaggtg taaaataaaa taggcatagt
    11701 ggtatggaag ttcactatgc ctattttgca aaatttccgc tcaattcaac cgcttgtagt
    11761 taataagtgg cattattttc tggattattc tgcttagtcg ccattctaag taacgtataa
    11821 cctaaaatcg ctgaaaccgc agagccgagt aaaataccta atcgagctag agcggtaata
    11881 ctttccccag cctcacttac atcaaaggca agacttgcta agaacatcga cattgtaaaa
    11941 ccgataccgc atagcaccgc aatggcaaaa atttgcttaa aattaacccc ttgcggcagg
    12001 ctggcaaggc ggaataatac tgctaaataa ctaaaaccga atacacctaa ggttttgccg
    12061 ataattaagc cgagcgcaat acctaacggc aatggtgatg agagttgctc cattcccatt
    12121 tctaataagt aaacgccggc attagcgaag gcgaagatcg gtaaaatcat ataagcacac
    12181 caaggggcaa gaatatgttc tagctcctct gccggtcgtt ccccatgttt acctttcagc
    12241 ggtacgcaga agccgatgat cacgccggca agggtagcgt gaacccctga ttttaaaacg
    12301 gaagtccata gcactaagcc tactaccaaa taagcacaga gtgaggtcac tttaaagcgg
    12361 ttaagtagca ctaagccgat aatcgcaatc gcggcaatag ttaaggcttg cacactgatt
    12421 tcttgcgaat agaaaatggc gattaccaca atcgctccta gatcatcaat aatcgctaat
    12481 gccaacaaaa atgcctttaa agccaatggc acacgagtgc ctaataacgc cacaatgccc
    12541 accgcaaagg caatatcagt tgccattgga attgcccaac cattatgata ttcagggtgg
    12601 ttttgattaa tcgcccaata aataatcgct ggaacaatca taccacctac tgctgctatc
    12661 gcaggaaaaa tggctttctt ataacttgaa agcgaacctt caaacaactc tttttttact
    12721 tccagcccca ccaacgtaaa aaacactgcc attaagccat cgttaaccca gagtaatagc
    12781 ggtttattca aggcaaattc accaaactgt acactaacaa tcgtttgtaa aaactcaaaa
    12841 tagaaatcac ttaaggaggt gttggcgcac acaattgctg caaaagcaaa aaccagaagc
    12901 aatacgcctc ctgaggcttc agatttt
//
LOCUS       DANJWC010000060        12874 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.060, whole genome shotgun sequence.
ACCESSION   DANJWC010000060 DANJWC010000000
VERSION     DANJWC010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12874
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.060"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            144..1247
                     /locus_tag="PWX02_001232"
     CDS             144..1247
                     /locus_tag="PWX02_001232"
                     /inference="COORDINATES: protein motif:HMM:NF024808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL1729250.1"
                     /translation="MLTGIQVNIDTYSFEQIPAVLENDQVEKKYDLVRIDMESLPYFA
                     EKYFLPLNSIGREHLELCFSASIIERFCLFQDKLYAIPFDPSIQMLFYRDDIFNDVKV
                     KRLFYEKNKKELSLPNNFEQFNQLSDFFKNHLPEEFGVKYGSALIVNDEGTLASEFLM
                     RYYALSDSIFMENVLKLEMQSAKKALKQLKAFYQSAKLLEQGWWKDAVELFNLGEIPM
                     LIVYMNHFLPLSHKGLMLPLGVSTLPNNRALMGGGSLAIMKQTQKIAACETFFNWFLD
                     NTTQEQYVQLGGSSAKRDIVENQNITKYLPWLPLALKGDFSGVRENSNSRQEAINLRQ
                     AENIIGGVVNVYLTTDMNEVEVVNLINARLSQL"
     gene            complement(1293..2459)
                     /locus_tag="PWX02_001233"
     CDS             complement(1293..2459)
                     /locus_tag="PWX02_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1729251.1"
                     /translation="MFNVSRLLIMMFLQYFTQGAWNMTIGAVLADYGMKNSIGSTYAL
                     LGLATLISPLFIGMIADRFLAAQKTIAILHLLNSVILYQIPSTILHNEYSQFFMLIFI
                     VGLLYYPTTALSNSITFQHLSDSRNFPFIRIFGNIGFITAGLIMGYFNIFDQVIVFEI
                     AAFSSLICGLYCFTLPNTPPAARHSVINWKTLLCLDAFILFRDKFFTIFMAVTMLLML
                     TKTSYSTYISVYLQDFQLNAANMMQIAVISEVIFMLLLSLSIKKFGFKWVMGMGIIAW
                     FIRTYLLSIAATATTPLTYIVISLALQGVCWTFFFATGDVYINTRQMIIFERKHKVYA
                     SCFLTILECWLPLILSALSIIMLSRSINCLSLLSNGQNFGYIQVRLHWLPVFYF"
     gene            complement(2535..3593)
                     /locus_tag="PWX02_001234"
     CDS             complement(2535..3593)
                     /locus_tag="PWX02_001234"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005705912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HDL1729252.1"
                     /translation="MFMQFDINEHPHRRYNPLKDEWILVSPHRAKRPWSGQVDKAPTD
                     ELPSYDPTCFLCPNNNRVSGEQNPDYKGTFVFSNDFAALMPDSPNSPETSHPLLKIQG
                     VQGLARVICFSPDHSKTLPELPITAIKGIVDTWNEQLEELSKDYVWVQVFENKGAMMG
                     CSQPHPHGQIWANSFLPNEIEKKDKNLVRYYQKNGSNLLLDYAQLEMNDGQRTVIETE
                     HWIAVVPYWATWSFETMLMPKKRHIKRMNELTESEKQDLTYAIKLLTTRYDNLFECSF
                     PYSMGWHFAPFFKDGRDIEHWQLHATFYPPLLRSATVRKFMVGYEMLAETQRDLTPEQ
                     AAQRLASLSDVHYKQKEN"
     gene            complement(3664..5082)
                     /gene="uhpT"
                     /locus_tag="PWX02_001235"
     CDS             complement(3664..5082)
                     /gene="uhpT"
                     /locus_tag="PWX02_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HDL1729253.1"
                     /translation="MAGFFDIKKIPPIGLTTAQQRKLWLRQFLKAFMVVFLAYMAMYL
                     IRNNFKAAQPFLKEQYGMSTLELGYIGLAFSIVYGIGKTALGYFIDGKNTKRIISALL
                     IGSSIAVMLMGFILSAFGSVVGVFIVLWGLNGVFQSAGGPASYSTIYRWVPRTRRATA
                     MGLRNSSHNIGGAIAGGLALWGANTFFAGNIAGMFIFPAVIALIIGIVGLYWGKDDPE
                     ELGWNRSEEIFEEPIEEENAAAETMTKWEIFKKYLLSNPWVWVLCVANVFGYILRIGI
                     DNWAPLYVRETLHFNDFDAVNTILWFEAGAFIGSLFWGTLADFFKGRTAAVGTVCLAI
                     VFFVIDFYRDGTSVMAVNIALFALGAMIFGTLTLIPLSIINLAPKRGVSVANGMAGTF
                     GYLFGDSTAKVGLALIADPKREGLTVFGHNLHGWRDVFIVFNISCVIAVVLLFLVALV
                     EERKIRSIQKAKELGLKVAKVD"
     gene            complement(5109..6080)
                     /locus_tag="PWX02_001236"
     CDS             complement(5109..6080)
                     /locus_tag="PWX02_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HDL1729254.1"
                     /translation="MKVIKTVATTLLLSLFSLSSFADSATQKILDNFQQYPFSQITVT
                     HRAQAGVESRIFPENSLAAINLAINRGVGIIEIDPRLTADGHYVLLHDETLDRTTNVE
                     EVFPQYKEKDGQYAGQVLLSKLTLEQVKQLKLTDGFDGQIHQVPTLKEVLAAAKGRVF
                     LDLDLKEIDVEKLSALIKEFGNDNLLAYNSNAELLKQVTDKTGVIPLPINTPITKDGD
                     PIADFHKFQKMWGDDFKIMHTFMSDVTPELVALADKHKVRLWINTLNDADGAAERFDY
                     SLWKSYLGSGANVFQSDFSIELTRFIELRNSCLTLKNVQGCEVFTEH"
     gene            complement(6426..8096)
                     /gene="glnS"
                     /locus_tag="PWX02_001237"
     CDS             complement(6426..8096)
                     /gene="glnS"
                     /locus_tag="PWX02_001237"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HDL1729255.1"
                     /translation="MSEEILTAETGTRANFITHIIDEDLASGKHNNVYTRFPPEPNGY
                     LHIGHAKSICLNFGIAQDYNGLCNLRFDDTNPVKEDVEYVDSIKQDVEWLGFKWEGEP
                     RYASDYFDQLYGYAIELINKGLAYVDELSPDEMREYRGTLTEPGKNSPYRDRSVEENL
                     ALFEKMKNGEFAEGKACLRAKIDMASPFIVMRDPVLYRVKFAHHHQTGDKWCIYPMYD
                     FTHCISDAIERITHSLCTLEFQDNRRLYDWVLDNISIERPLPHQYEFSRLNLEGTLTS
                     KRKLLKLVTDGIVDGWNDPRMPTISGLRRRGYTPASLREFCRRIGVTKQDNVVEYSAL
                     EACIREDLNENAPRAMAVINPVRVVIENFGEKEILKAPNHPNRPELGERDLPFTREIY
                     IDEADFREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDASGRISTIFCTYDPDTLGK
                     NPADGRKVKGVIHWVSAEDNKPAEFRIYDRLFTVPNPGAEEDINAVLNPESLVVKHGF
                     VESSLAHAKPEQGYQFEREGYYCLDSKDGSADNLVFNLTVSLKESVAF"
     gene            complement(8257..8700)
                     /locus_tag="PWX02_001238"
     CDS             complement(8257..8700)
                     /locus_tag="PWX02_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDL1729256.1"
                     /translation="MNLQQIQDRLALKDLVDTFSNLADEKKVAEQMPLFTQDAVVNTY
                     IGGKLVFEMHGVAEIEQVFTTFLTPFHSVYHLNGQHTVTFIDENNATAINYCAVKLVE
                     TKEGKEILHDHSVRYADTYVKQEGKWRIAKRIANFMISESREIRQ"
     gene            complement(8751..9326)
                     /locus_tag="PWX02_001239"
     CDS             complement(8751..9326)
                     /locus_tag="PWX02_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDL1729257.1"
                     /translation="MKQILILGASGSLANVVIPELLKDNNVQLTLFARRPQSVAKFET
                     ERVKIVQGDVLDFHALTQAMQGVDAVYAGLAGDLKAMAGNVVNAMNEAQVKRLVWISS
                     MGIYGETGEDHGAILEPYRQSAAVIEASELDYTVVRPGWFTNGSEVQYQLTHKGEPFK
                     GHSVSRKSIADFVARILRENSEIRESVGIAA"
     gene            9433..10323
                     /locus_tag="PWX02_001240"
     CDS             9433..10323
                     /locus_tag="PWX02_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL1729258.1"
                     /translation="MKENLNDLRAFLLVAQTSSFTKAAAQMGVSQSALSHSIRGIEER
                     LQIKLFHRTTRSISTTEAGERLYQRLLPLFDEIDQEVNGLSEFRNALKGGLRINGNEH
                     VFRDVLADKFAEFVKRYPEVALELVAEDRFVDIVAERFDAGIRLGADVAKDMIALRIS
                     PDVKMTAAASPNYLAQHGTPKTPFDLTEHHCLKHRLATLGGILAWEFRDPITKKIVKF
                     QPQGNLTANNGFLLQHYAKQGLGILWSPQDSIQTELDNGSLVPILTDWAMQYEGYHLY
                     YPNRRQNSPLLKALIDVLRG"
     gene            complement(10390..10956)
                     /gene="efp"
                     /locus_tag="PWX02_001241"
     CDS             complement(10390..10956)
                     /gene="efp"
                     /locus_tag="PWX02_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDL1729259.1"
                     /translation="MASYSTNDFKKGLKFIQDGEPCVIVENEFVKPGKGQAFTRTKIR
                     KLISGKVLEINFKSGTSVEAADVVDSNYNYSYSDGDFWYFMHPETFEQISVDEKALGD
                     NVKWLVDNAECIVTLWNGSAIAVTPPNFVELEIVETDPGLKGDTAGTGGKPATLSTGA
                     VVNVPLFVQIGEVIRVDTRSGEYVSRVK"
     gene            11123..11692
                     /locus_tag="PWX02_001242"
     CDS             11123..11692
                     /locus_tag="PWX02_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729260.1"
                     /translation="MKKLITLSAILAVSVATVAEAKRGGGSFRSVSPSKAVATKPVAS
                     QKSTTNQQTQQKDATFANTPNSATAQPQVRQGSTMANVAMGAAAGYILGDMMSSNAHA
                     NQPSQPADANAQAVNSAQNVATGAVPAIAEFKSIGGQIDPFLVEKTDGYRRYCISGVQ
                     YLVAAQGVQTSPTVMVNPNGTPLQCNLIP"
     gene            11746..12738
                     /gene="epmB"
                     /locus_tag="PWX02_001243"
     CDS             11746..12738
                     /gene="epmB"
                     /locus_tag="PWX02_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDL1729261.1"
                     /translation="MQPIQIKPRWLTELAQAFNNPIDLLQFLELNPNDFEGDIAARKL
                     FALRVPRMFAEKMEKGNPNDPLFLQAMSLQAEFIEAEGFVVDPLEEQQSPAPNILHKY
                     HNRLLFMLKNSCAINCRYCFRRHFPYEEVKSGKAVWQQGLTYIAEHPELEEVILSGGD
                     PLMAKDQDLDWILTQLEQISHIKTLRIHSRLPVVIPNRITTELCERLSKSRLNVVLVT
                     HINHANEIDAVFANKMAQLKKAGVVLLNQSVMLKGVNDNAQTLKRLSDKLFEYGILPY
                     YLHLFDKVAGASHFYIEDEQAGEIYRELQRITSGYLVPKLAREIAKEPNKILKG"
BASE COUNT         3885 a         2609 c         2596 g         3784 t
ORIGIN      
        1 atgctatcat aaaagagcat cactttgggt ttccaaatat tatagaaagc tcaaataata
       61 cactacctca tcaagaaatt aatttacttg cagtccagac accatcaaca gaatctctta
      121 gaaaattatt accttatttt gaaatgctta ccgggattca agtcaatatt gatacatact
      181 catttgagca aattcctgcg gtgctggaaa atgatcaagt agagaaaaaa tatgatctgg
      241 tgcgaattga tatggaaagt ctgccttatt ttgcggaaaa atatttctta ccccttaata
      301 gtataggaag agaacaccta gaattatgtt tttccgcaag tattattgaa cgcttttgtc
      361 tgtttcaaga taaactctat gctattccgt ttgacccaag catacagatg ttattctatc
      421 gagacgatat ttttaatgat gttaaagtaa aaagattgtt ttatgaaaaa aacaaaaaag
      481 aattatcttt gccaaataat ttcgagcaat ttaaccaact gtctgatttc tttaaaaatc
      541 atttgcctga agagttcggc gtaaaatatg gctctgcgtt aattgtcaat gatgagggaa
      601 cattagcctc tgaattttta atgcgttatt atgcgttatc agattctatt tttatggaaa
      661 atgtactgaa gttagaaatg cagtcggcta aaaaagcgtt aaaacaactt aaagcctttt
      721 atcagtcagc gaaattattg gagcaaggct ggtggaaaga tgcggttgaa ttgtttaatt
      781 taggcgaaat tccaatgctt attgtgtata tgaaccattt cttaccatta tcacataaag
      841 ggttaatgtt gccacttggc gtttctacat taccgaataa tcgagcatta atggggggcg
      901 gctcattagc cataatgaaa caaacacaga aaatagcagc ctgtgaaaca ttttttaatt
      961 ggtttttaga caataccacc caagaacaat atgttcagtt agggggcagt tcagctaaaa
     1021 gagatattgt agaaaatcag aatataacta agtatttgcc ttggttacct ttagcattaa
     1081 aaggcgattt cagcggtgta agagagaata gtaatagtcg tcaagaagcg attaatttaa
     1141 gacaagctga aaatattata ggtggtgttg ttaatgtgta tttaaccact gatatgaatg
     1201 aagttgaggt ggtgaacttg attaatgcaa ggttaagcca gctatgaata aaacagcttg
     1261 ctattaggtc gccttttcat ttttaaatag cgttaaaaat aaaataccgg caaccaatgc
     1321 aatcgcactt ggatataacc aaaattctga ccattgctca gcaatgagag gcagttgatg
     1381 ctgcgtgata acattattat agataatgcc gataatataa gaggcaacca acactccaat
     1441 atcgttagaa agcatgaagc gtaaactttg tgcttgcgtt cgaatatgat catttgcctt
     1501 gtattaatat agacatcgcc tgtggcaaag aagaatgtcc aacatactcc ttgtaatgcc
     1561 aagcttataa cgatgtaagt tagcggtgtt gtagcagttg cggcaatcga cagtaaataa
     1621 gtgcgaataa accaagcaat aatgcccatc cccattaccc atttaaagcc aaatttcttg
     1681 atacttaagc taagcaatag cataaaaata acttctgata taactgctat ttgcatcata
     1741 ttggcggcat tgagctgaaa gtcttgcaga taaaccgaaa tataagtact ataagaggtt
     1801 ttagtcagca ttaataacat cgttaccgcc ataaaaatgg taaagaactt atctcggaat
     1861 agaataaatg cgtctagaca taataaggtt ttccagttta ttacgctatg acgggcagcc
     1921 ggcggtgtat tcggtaaggt gaaacaatat aagccacata tcaacgatga gaatgctgct
     1981 atttcaaaaa cgatcacttg gtcaaaaata ttgaaatagc ccataataag tccggcagta
     2041 ataaagccaa tattgccaaa aatacgaatg aatggaaaat ttctactgtc agataaatgc
     2101 tgaaatgtaa tgctattaga aagtgcggtt gtggggtaat agagcaatcc cactataaaa
     2161 atcagcataa agaattgaga atattcatta tgtaatatag ttgagggaat ttgatacaaa
     2221 atcacactgt tcagtaggtg tagaatagcg atagttttct gtgctgccaa aaatctatcg
     2281 gcgatcattc caataaatag cggtgaaata agggttgcaa gtccaagtaa agcataggta
     2341 gaaccaatgc tgtttttcat tccataatct gctaataccg ccccaatggt catattccac
     2401 gccccttggg tgaagtattg cagaaacatc ataattaaaa gtcttgaaac attgaacata
     2461 tcaaccgtat tttctaaagg aggaatatat ttagtggcta aaattgttat ctaaatatat
     2521 tccgtgttga aaaattaatt ttctttttgc ttatagtgaa catcgctcaa agaggctaaa
     2581 cgctgtgcag cttgctcagg tgtgaggtct ctttgggttt cggctaacat ttcataacct
     2641 accataaatt tacgcactgt cgcagagcgg agcagtggcg gatagaatgt agcgtgcagt
     2701 tgccaatgtt caatatctcg tccatcttta aagaatggag caaaatgcca ccccatagaa
     2761 taagggaaag agcattcaaa taagttatca taacgagtcg tgagtaattt aatcgcataa
     2821 gttaaatcct gtttttctga ctcggtaagt tcattcattc ttttaatatg gcgttttttc
     2881 ggcattagca ttgtttcaaa cgaccacgta gcccaatagg gtacaactgc gatccaatgt
     2941 tctgtttcga tcaccgttct ttgaccatca ttcatctcaa gctgagcata atctagcaaa
     3001 agatttgagc catttttctg ataatagcga actaaatttt tatctttttt ctcaatttcg
     3061 ttaggtaaaa aagaatttgc ccaaatttgc ccgtgtgggt gaggctggga acagcccatc
     3121 attgcccctt tattttcaaa tacttgcacc catacataat ctttgcttaa ttcttcaagc
     3181 tgctcattcc aagtatcaac aatgccttta attgcggtaa taggtaattc aggcaaggtt
     3241 ttgctgtgat caggcgaaaa gcagatgact ctcgccagcc cttgaacacc ttgaatctta
     3301 aggagtgggt gagatgtttc cggcgagttg ggcgaatccg gcattaaggc ggcaaaatca
     3361 ttgctgaata caaaggtgcc tttatagtcc ggattttgct cccctgaaac tcggttgtta
     3421 ttcgggcata aaaaacaagt gggatcataa ctcggtaatt catcagtcgg tgctttatcc
     3481 acctgcccgc tccaagggcg tttggctcgg tgtggagaga ctaaaatcca ctcatctttc
     3541 aacggattat aacggcgatg agggtgttca ttaatatcga attgcataaa catatcctga
     3601 ttataataga cggaacagac cgcttgccta aacaagcggt catttttcat tgaaaatttg
     3661 caattaatct acttttgcga cttttaatcc taattctttt gctttttgaa tactacggat
     3721 tttacgttct tcaacaagag caacaaggaa taaaagtacc acggcaatca cgcaagagat
     3781 gttaaagacg atgaatacat cacgccaacc gtgtaaatta tgtccgaata cggttaaacc
     3841 ttcacgtttt ggatctgcaa ttaacgctaa accgactttt gcggtggaat cgccaaataa
     3901 gtaaccaaat gtacctgcca taccgtttgc aacgcttacc cctcttttcg gtgctaagtt
     3961 aataattgaa agtgggatta aggttaaagt accgaagatc atcgcaccta aggcaaataa
     4021 tgcgatattt acagccatta cacttgtgcc gtctcggtag aaatcgatca caaagaacac
     4081 aatcgctagg cagactgtac ctactgcggc agtacgacct ttgaagaagt cggctaatgt
     4141 tccccagaat aagctaccga tgaatgctcc ggcttcaaac cataagatag tgtttactgc
     4201 atcgaaatca ttgaagtgta aggtttcacg aacgtagagt ggtgcccagt tatcaatccc
     4261 gattcgtaaa atataaccga aaacatttgc cacacaaagc acccaaaccc aagggttaga
     4321 caacaggtat tttttaaaga tttcccattt tgtcattgtt tcggctgccg cattttcttc
     4381 ttcgattggc tcttcaaaga tttcttcact gcgattccag cctaactctt ccgggtcatc
     4441 tttaccccaa tacaagccaa caataccaat gataagtgcg atcaccgcag ggaaaataaa
     4501 catacctgca atgttaccgg caaagaaagt attggctccc catagggcta aaccaccggc
     4561 aatagcacca ccgatattat gcgagctatt ccgtaaaccc attgcggttg cacgtcttgt
     4621 tcttggtacc caacgataaa ttgtgctata ggaagcaggg cctccggcag attggaacac
     4681 cccgttcaat ccccataaca caatgaatac acctacaacc gaaccaaagg cacttaagat
     4741 aaaacccatt agcataacag caatagatga tccaattaat aaggcagaaa taatgcgttt
     4801 ggtatttttg ccgtcaataa agtagcctaa cgctgtttta ccaatcccat atacaatcga
     4861 gaaagccaag ccgatatagc ccaactcaag agtggacata ccatattgtt cttttaagaa
     4921 aggttgtgca gctttaaagt tattgcgaat taaatacatc gccatataag ctaagaatac
     4981 caccataaag gctttcaaga actgacgtag ccatagctta cgttgttgtg ctgtggtcaa
     5041 acctattggt gggatttttt taatatcaaa gaagccagcc atataaactc caaattatcg
     5101 gttaaagatt aatgttcggt aaatacttca caaccttgta catttttcaa ggttaagcaa
     5161 gaattacgca attcaataaa acgggttaat tctatagaaa agtcggattg gaatacgttt
     5221 gctccagagc ctagataact tttccataag gaataatcaa atctttcggc agccccatcg
     5281 gcatcattga gtgtattgat ccaaagacgc actttgtgct tatcagctaa tgccactaat
     5341 tcaggggtta catcagacat aaaagtatgc ataattttga aatcgtcccc ccacattttt
     5401 tggaatttgt ggaagtctgc aatcggatcg ccatctttgg taatcggggt attaattggc
     5461 agaggaatta ctccggtttt atctgtaact tgttttaaaa gctcagcatt gctgttgtaa
     5521 gccagtaaat tatcattgcc gaattcctta attaacgcag acagtttctc tacatcaatt
     5581 tctttcaaat ccaaatcaag aaatactctg ccttttgcag cggcaagcac ttcttttagg
     5641 gttggcactt gatgaatttg cccatcaaaa ccgtctgtca attttaattg ttttacctgc
     5701 tctaacgtga gcttagaaag caaaacctga ccggcatatt gaccgtcttt ctctttatat
     5761 tgtggaaaga cctcttctac gttagtggtt ctatccagcg tttcatcgtg taataaaacg
     5821 tagtgaccat cagcggttaa acgaggatcg atttcaatga tgcctacgcc ccgattaatc
     5881 gctaaattaa tggcagctag agagttctct gggaaaatac ggctttctac accggcttgc
     5941 gctcggtggg tgacggtaat ttgagagaaa gggtattgtt ggaaattatc caaaatcttc
     6001 tgtgttgcgg agtccgcaaa actagatagt gagaataaag atagtagcaa ggtggttgct
     6061 actgttttga ttactttcat agtaactcct taatatagtt tggcgaccat ctcaaaataa
     6121 ttgaaacgtt tcatttttaa tcttaaaatt taaaatgaaa cgtttcaatt agagaaggtg
     6181 gattttgtga ctgagatcac aaatttaaac attggtctgt atttttgaga gattttgttt
     6241 gaaactgccg gcaaattcgc ttaaccttaa ggcaacaaga ataagtgttg tacctagcaa
     6301 cctaacacat aaatttgctt tccgagtggc cccaagaatt ggggcttttt ttatgcttga
     6361 tgagtcgttt tgagagtaaa aaaaatacca gcatatttgc tggtatttga atgaagagag
     6421 ttagattaga acgctacgct ttcttttaag ctgacagtta agttaaatac aaggttgtct
     6481 gccgaaccat ctttgctgtc taagcagtag taaccttcac gctcgaattg gtagccttgt
     6541 tccggtttgg cgtgagctaa acttgactca acaaaaccat gcttaaccac taatgattca
     6601 ggatttagca ccgcattgat gtcttcttcc gcccccgggt ttggtactgt aaataggcgg
     6661 tcgtaaatgc ggaactctgc cggtttgtta tcttcagcag atacccaatg aatcacgcct
     6721 tttactttgc gaccatcagc cggatttttg cccaatgtat ccgggtcgta tgtgcaaaaa
     6781 atagttgaaa ttcgaccgct tgcatctttt tccacacgct cagctttaat cacataggca
     6841 ttgcgtaaac gaacttcttt gcctaagact aaacgtttat attgcttatt ggcttcttca
     6901 cggaagtcgg cttcatcaat ataaatctca cgggtgaacg gtaaatcacg ctcgccaagc
     6961 tctggacgat ttgggtgatt aggggctttt aatatttcct tttcaccgaa gttttcaatc
     7021 accactcgaa ccgggttaat caccgccatc gcgcgtggtg cgttttcatt taagtcttca
     7081 cgaatgcacg cttcaagggc agagtattcc accacgttat cttgcttggt taccccaata
     7141 cggcgacaaa attcacgtaa agacgctggg gtataaccac ggcgacgtaa gccggaaatg
     7201 gttggcatac gagggtcatt ccaaccatca acaatgccgt ctgtgactaa ttttaataat
     7261 ttacgcttag aggttaaagt accttctaaa tttaaacgtg aaaattcgta ttggtgcggt
     7321 aacggtcttt caatcgaaat attgtccagc acccaatcat ataagcggcg gttatcttgg
     7381 aactctagcg tacatagcga gtgagtgatt cgctcaatcg catcggaaat gcagtgggta
     7441 aaatcgtaca tcgggtagat gcaccattta tcaccggttt gatgatggtg agcaaatttc
     7501 acacggtaaa gcaccggatc tcgcatcaca ataaacgggg acgccatatc aatttttgct
     7561 ctcaagcagg ctttcccttc cgcaaactct ccgtttttca ttttttcgaa taacgctaag
     7621 ttttcttcca cgctacgatc acggtaaggg ctgtttttgc ccggctcggt taaagtacca
     7681 cggtattcac gcatttcatc aggcgataat tcatcaacat aggctaatcc tttgttgatt
     7741 aactcaatcg catagccata aagctggtca aaatagtctg aggcataacg aggctcaccc
     7801 tcccatttaa aacctagcca ctccacatct tgcttaattg aatctacata ttccacgtct
     7861 tctttaaccg ggttggtatc atcaaaacgc aagttacata aaccgttata atcttgagcg
     7921 ataccaaaat ttaaacagat agatttagcg tgtccgatat gcaaataacc gtttggttca
     7981 ggtggaaaac gagtgtaaac attgttgtgc tttccgcttg ctaaatcttc atcaataata
     8041 tgggtaataa aattggctcg agtgccagtt tctgccgtta aaatttcttc gctcatagtt
     8101 ctctctaaat gtatcaataa aaataacgga ttattctaca cttaattgaa acagacttaa
     8161 agactaaagc aaaattcctc ggtttcctcc aaagaatttt gctgttttag taagatgcaa
     8221 tggaattgca aatttttagc taaatttaac cgcttgttat tgcctgattt cacggctttc
     8281 gctgatcata aaattcgcaa tccgtttggc aatccgccat ttaccctctt gtttaacata
     8341 ggtatcggca taacgcacgc tgtgatcgtg caagatctct ttgccctctt tggtttctac
     8401 cagtttcacc gcacaatagt tgatcgctgt ggcgttattt tcatcaataa aagtcacggt
     8461 atgctgaccg tttaaatgat aaacgctgtg aaacggggtt aaaaaggtgg taaacacctg
     8521 ttcgatttct gccacgccgt gcatttcaaa caccaatttt ccgccgatgt aggtgtttac
     8581 gacggcatct tgcgtgaaaa gcggcatctg ctcggcaacc tttttttcat cggcaaggtt
     8641 agagaatgta tctactaaat cttttagtgc taaacgatct tggatttgtt gtaagttcat
     8701 tgtgttttcc ttctgatttt tgcaaatttt ctttggtttt tcaccgcttg ttaggctgca
     8761 ataccaacac tttcacggat ttcgctgttt tcacgcaaaa tgcgagccac aaaatcggca
     8821 atggatttgc gggaaacgct gtgtcctttg aacggttcgc ctttgtgagt cagctgatat
     8881 tgcacttccg aaccgttggt aaaccagccc gggcggacaa cggtataatc caactcgctg
     8941 gcttcaatca cggctgccga ttggcggtaa ggctctaaaa ttgccccgtg atcttcgccg
     9001 gtttcgccat aaatccccat tgagctaatc caaaccaagc gttttacctg agcttcattc
     9061 attgcgttca ccacatttcc cgccatcgct tttaagtcgc cggcaagtcc agcgtaaacg
     9121 gcatcaacgc cttgcattgc ttgggttaat gcgtggaaat ccaacacatc gccttgcaca
     9181 attttgacgc gttcagtttc aaatttcgct actgattgcg gacgacgggc aaaaagggtt
     9241 aattgcacat tattgtcttt tagcagttca ggaatcacga cattcgctag gctaccgctt
     9301 gctcctaaaa ttaagatctg tttcattgct gttctccatt gaatgaagtc agtatagcgg
     9361 tgtagattgc atttgattag tagggtaaaa tcgcttgaag taatgagaaa aattcattaa
     9421 taaggtggtt aaatgaaaga aaacctcaac gatttaagag cttttttgtt ggtagcacaa
     9481 acgagcagtt ttaccaaagc agcagcacag atgggcgtgt cgcagtcggc gttgtcgcac
     9541 agcattcggg ggattgaaga acggttgcaa atcaagctgt ttcatcgcac cactcgtagc
     9601 atttcaacca ccgaggcagg ggagaggctg tatcaacggc tgttaccgct gtttgatgaa
     9661 atcgaccaag aagtaaatgg gctgagtgag tttcgcaatg cgttaaaagg cgggctgaga
     9721 attaacggta atgaacacgt tttccgtgat gtgctggcag ataaattcgc tgagtttgtc
     9781 aaacgttacc ctgaagtagc attggagcta gtggcggaag atcgctttgt agatattgtc
     9841 gccgagcgtt ttgatgccgg tattcggctt ggggcagatg tggcgaaaga tatgattgca
     9901 ctacgcattt cgcccgatgt gaaaatgacc gcagctgcca gccccaacta tttggcacag
     9961 cacggcacgc cgaaaacgcc ctttgattta accgaacatc attgtctcaa acatcgtctt
    10021 gcgacattgg gcggcattct ggcgtgggaa tttcgggatc ccatcaccaa gaaaatcgtt
    10081 aaatttcaac cgcagggcaa tcttactgcc aataacggct tcttgctgca acactatgcc
    10141 aaacaaggtt taggcattct ttggagtccg caagacagca ttcaaaccga gctggataac
    10201 ggcagcctcg tgccgatttt aaccgactgg gcaatgcagt atgagggcta tcatctctat
    10261 taccccaacc gtcgccaaaa ttcgcctttg ctgaaagcgt taattgatgt gctgagagga
    10321 taggtaaaaa tgagcggtcg tttttagcaa aaaattgacc aaaaaacgac cacttgtaga
    10381 gaagctaaat tatttcacac gagaaacata ttcgcctgaa cgggtatcca cacggataac
    10441 ttcaccgatt tgaacgaata aaggcacgtt taccacagca ccggtgctta atgttgccgg
    10501 tttaccgcct gtacctgcag tatcgccttt taagcccggg tcggtttcga cgatttctaa
    10561 ctcaacgaag tttggtggtg tgactgcaat tgctgaaccg ttccataaag tcacgataca
    10621 ttctgcatta tcaactaacc attttacgtt gtcgcctaac gctttttcat ctaccgagat
    10681 ttgttcaaag gtttcagggt gcataaagta ccagaaatca ccatcgctgt aagagtagtt
    10741 gtagttagaa tctactacat ctgctgcttc aaccgaagtg cctgatttga agttgatctc
    10801 taatactttg ccagagatta atttacggat tttagtacgg gtaaatgctt gacctttacc
    10861 cggttttacg aattcgtttt caacaattac acaaggttcg ccatcttgaa taaattttaa
    10921 acctttttta aagtcgttag tgctgtagct agccattatt tcctcaatta tttaaaaaat
    10981 aacaataaat taacaaattt tgaacccaac cctattgggt ttgtcaaaaa gagccatata
    11041 ataacctaaa ttcacaaaat tagcgaaaga tctcttgcga tttttgctat aatttttcaa
    11101 actttattga taaggaatgc gaatgaaaaa actgattact ctttctgcga ttttagccgt
    11161 ttctgttgca accgtggcag aggcaaaacg tggcggtggg agcttccgtt cagttagccc
    11221 gtcaaaagcg gtagcaacta agccggtagc ttctcaaaaa tcaaccacaa atcagcaaac
    11281 gcaacaaaaa gatgctactt ttgccaatac gccaaacagt gcaactgccc agccacaagt
    11341 gcgtcaaggc agcacgatgg caaatgtagc gatgggggca gcagccggtt atattttagg
    11401 tgatatgatg tcatcaaatg cacatgcaaa tcagcctagc cagcctgcgg atgcaaatgc
    11461 acaagcggtc aattctgcac aaaatgttgc aactggagca gttcctgcta ttgcagaatt
    11521 taagagtatt ggcggtcaaa tcgatccgtt tttagtagaa aaaaccgatg gttatcgccg
    11581 ttattgtatt tcaggcgtgc aatacctagt cgctgcacaa ggtgtacaaa cctcaccaac
    11641 tgtaatggtt aatccaaatg gcacgccatt acaatgtaac ttaattccat aattattttt
    11701 atttgcgggc aggcaaaaac ctgccccttg tatctacaat attcaatgca accaattcaa
    11761 atcaaaccgc gttggctaac cgagttggct caagccttta ataaccctat cgacttatta
    11821 caatttcttg aactcaatcc aaatgatttt gagggcgata tagctgcccg taaattattt
    11881 gctcttcgag tgccaagaat gttcgccgaa aaaatggaaa agggtaaccc aaacgatccg
    11941 ctttttttgc aagcaatgtc tttacaggcg gaatttattg aggcagaagg gtttgtggta
    12001 gatccattgg aagagcaaca aagcccagca ccgaatattt tacataaata ccataaccgt
    12061 ttgttattta tgctaaaaaa cagttgtgcg attaactgcc gctactgttt tcgccgtcat
    12121 ttcccttatg aggaagtgaa aagcggtaaa gctgtgtggc aacagggact aacttatatt
    12181 gctgagcatc ccgagctgga ggaagtgatt ctgtccggtg gtgatccgct gatggcaaaa
    12241 gatcaggact tagattggat attaacgcaa ttagagcaga tttcgcatat caaaaccctg
    12301 cgtattcatt cacgcttgcc ggtcgttatt cccaatcgaa ttacgacaga attatgtgaa
    12361 cggttatcaa aatctcgctt gaatgtcgtg ttggttaccc atattaatca cgccaatgaa
    12421 attgatgctg tttttgcaaa caaaatggct caactgaaaa aagccggcgt tgtattgctg
    12481 aatcaatcgg tgatgttaaa aggtgtaaac gataatgctc aaaccttaaa aaggttaagt
    12541 gataaacttt ttgaatatgg gattttgcct tactatttgc atttatttga taaagtggca
    12601 ggggcgagcc atttctatat tgaagatgaa caggctggtg agatttaccg agaattacag
    12661 cgtatcactt caggctatct tgtgccaaag ctggcaagag aaatagcgaa agagccaaat
    12721 aaaattttga aaggttagct taaacagaaa tcaagaaaca aaaggcgaaa atggtaaaat
    12781 tttcgccttt tttaaccgct tgttgcaagc ggttaaattc tcttactttt ttgcaaatta
    12841 gaagttcgca ttgcgtggag cacgtgggaa tggg
//
LOCUS       DANJWC010000061        12719 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.061, whole genome shotgun sequence.
ACCESSION   DANJWC010000061 DANJWC010000000
VERSION     DANJWC010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12719
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.061"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..1259)
                     /locus_tag="PWX02_001244"
     CDS             complement(<1..1259)
                     /locus_tag="PWX02_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL1729262.1"
                     /translation="MSKLSDFISIKHGFAFKGEFITTEENANCLITPVNFSIGGGFKS
                     DKFKYYTGEIPEKYILQPNDLIVTMTDLSKQADTLGYPALVPNISGKKMLHNQRIGLV
                     EFLDNELDKEYLYFLMRTKEYRHQILSTATGATVHHTSPSKILDFEFEKPDLQTQKLI
                     AQYLMILEEKIQLNTQTNQTLEAIAQAIFKSWFVDFDPVRAKAQAILDGKTSDEANLS
                     AMAVFSGKAIEDLSQTEYQELWEIADAFPSEFGDEGLPIGWKFNQADNLFDVGIGKTP
                     PRKESEWFSDNANDTEWISIKDMGNQGLFITESSEYLKVEAVDKFNIKRIPENTVILS
                     FKLTVGRVSITTKETTTNEAIAHFKIPSSSNLSSEFLYCYLKNFDFNNLGSTSSIATA
                     VNSKMIKEMEILEPSDLVINHFNEYIEG"
     gene            complement(1261..2919)
                     /locus_tag="PWX02_001245"
     CDS             complement(1261..2919)
                     /locus_tag="PWX02_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDL1729263.1"
                     /translation="MPNNTARPALDNTVSQAFLNDLDQTLWTAADKLRKNLDAANYKH
                     IVLGFIFLKYISDSFTDFQAKLKTQLTTPESELYLDPALFDEQEFSQILAEELEQRDY
                     YAAENIFWVPEQARWDNIKSLSKLNLGDELPWGDKFKGVSRLIDDAFEAIERENPKLK
                     GVLQRIAGFGVPDEMLTGLIDLFSRTNFTQPMHNGEPVHLQAKDILGHVYEYFLGQFA
                     LAEGKKGGQYFTPKSIVTLIVEMLKPYSGRIYDPAMGSGGFFVQADRFILAHQGNRNA
                     ISVYGQESNPNTRKLAVMNMAIRGIPFDFGDKPEDTLLNPLHIDKKMDVVMANPPFNQ
                     KAWWSESLANDPRWAYGTPPQGNANFAWLQHMIYHLSPKGKMALLLANGSMSSQTSGE
                     GDIRKNIVQADLVEAMIALPNQLFTNTQIPACIWIINKAKARKKEVLFINATQIGYMK
                     DRVLRDFTADDIAKISDTYHNWQKQDGYENIPAFCYCATLDEIAKNDFVLTAGRYVGA
                     AVEEDDGVPFAQKMQELTALLNEQFRQGRALEEKIVANLKGLGF"
     gene            3080..3457
                     /locus_tag="PWX02_001246"
     CDS             3080..3457
                     /locus_tag="PWX02_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HDL1729264.1"
                     /translation="MEYQFTHSIHGVVAKCSMDHEAFARWLNTEITGNPKELEAIFVE
                     IEKCRVAFPNHYECIFEGREYSLYFDYDEVMVKANNLDTAFDENEMEDGFQFYNEESI
                     AFCGLDDFEKLLKAYQYFVQTYH"
     gene            3550..4137
                     /gene="yihI"
                     /locus_tag="PWX02_001247"
     CDS             3550..4137
                     /gene="yihI"
                     /locus_tag="PWX02_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HDL1729265.1"
                     /translation="MSRTKKTRRITDIMPARKADKPAKPTPKLGGGKKLKLTRYELDV
                     LAREEKRKRKHKGLPTGSRNADPAEQKKAVVKEVKDPRIGSRKKVPLMVEFVNQPEKG
                     RTIKAVPVEPIKPTLSPEQELEQLENNECLNQLLDDLEAGKTLSAEDQKFMNECLDRI
                     DELMTELGIEYEDEEGDNGDALLRQFETMDINKFR"
     gene            4151..4618
                     /locus_tag="PWX02_001248"
     CDS             4151..4618
                     /locus_tag="PWX02_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2489 domain-containing protein"
                     /protein_id="HDL1729266.1"
                     /translation="MLRIFLMVLAALILISLGGYALHLMLKVRAQKKHEQALIEQAKQ
                     AQKDRYIRILDSIEVIARAMISEQCDFSEGVLRLKPLLDVLGRKLSDYTAMWALYGVV
                     ENMPILDERKNLKRNERMKLDLEREAKEAELESDIKNECYQLLKDIEEMRKAI"
     gene            4618..5985
                     /gene="hemN"
                     /locus_tag="PWX02_001249"
     CDS             4618..5985
                     /gene="hemN"
                     /locus_tag="PWX02_001249"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDL1729267.1"
                     /translation="MSEIIWDLALIQKYNHSGPRYTSYPTALEFNENYTNEDFIRAAA
                     RYPERPLSLYVHIPFCHKLCYFCGCNKVITRHRHKVEIYLDYLEREIKTRAPLFQNRL
                     VTQIHWGGGTPTYLDEDQSARLMQMLRANLDVSDDAEISIEMDPREIELSMLDHLRAI
                     GFNRLSMGIQDFDKEVQRLVNREQDEEFIFALMKRAKELGFVSTNVDLIYGLPKQNVE
                     SFMYTLQRVVELNPDRMSVFNYAHLPSRFAAQVKIKEELLPAPETKLTILQNSIEFLG
                     KNGYKFIGMDHFAKPDDELAIAQEKGILHRNFQGYTTQEECDLLGMGVSAISLLGDTY
                     AQNRKELQQYYADVETNGIALHKGIALSQDDCIRRDVIKALICNFKLDFATIEKQYGI
                     DFKRYFAEDLAFLAPLAEDELLTMNDDSIVVSPRGRLLIRNICLCFDVYSRQLARRQQ
                     FSRII"
     gene            6059..8392
                     /gene="lptD"
                     /locus_tag="PWX02_001250"
     CDS             6059..8392
                     /gene="lptD"
                     /locus_tag="PWX02_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HDL1729268.1"
                     /translation="MKQRYSLLSLAVMAACYSNSAAADLKQQCLLGVPHFQGEVVEGD
                     QLRMPVTIESDSAVINQPRDATYTGDVTIKQGNRSIWAEQVRVEQDGENARMAYLQGN
                     FNYQDNLIQATGADATINLLSKDANLTNANYQLVGRQGRGTAESGEFGNSKRTLKNAS
                     FTSCLPNDNSWQIEANEMIQHVDEEYAEMWHARFKVLGMPVFYSPYLQFPIGDRRRSG
                     LLIPSFERSSKNGWTYSQPFYWNIAPNMDSTITPTYYSRRGWQISPEFRYLTKIGQGL
                     VATEYMAKDRLSEYQPGNDDRKRYLFHWRHAMSFLTDWRFSVDYTKVSDRRYFSDFDS
                     DYGSSTDGYATQNFKLGYYQPNYNISISGKKFQTFDELDIGPYRVLPQIDFNYYKNDL
                     IKGGDFKFFAQAARFENDSKLMPKAWRFHAEPTLNFPLANRYGSLNLETKLYATYYQQ
                     TKGSESTEEIKKSVSRVLPQIKLDFKTVLESDKQLFSGYTQTLEPRVQYIYRPYKDQS
                     DIGSKAHRSVSLGYDSALLQNDYFSLFNDRRYSGLDRISSANLVTAGGTTRFFKEATG
                     EEVFNFSLGQTYYISPSKIDDTSDNSTKGHTTSWAAESNWKFHKNWNWHGSYQYDTRL
                     NQTALANMSLQYKPQSDKLIQLNYRYASKDYINQNLNTNKYGQSIKQVGGVLGWELTD
                     KVSVMTSYYHDLALKKPVEGQISLNYNTCCWAANVYVARRLVSTPVGSSDTINDFYYD
                     NKFGINFELRFGTNYSNGVTRMLKRGIIPYTEPYGIN"
     gene            complement(8534..11173)
                     /gene="ppc"
                     /locus_tag="PWX02_001251"
     CDS             complement(8534..11173)
                     /gene="ppc"
                     /locus_tag="PWX02_001251"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HDL1729269.1"
                     /translation="MNQQYSTIQHNIHMLGEFLGETIRDAQGNYILELIESIRVLSRN
                     SRAGDENAREQLLDKLANISNDDVLPVARAFSHFLNLTNIAEQHQTVSRHHIDESLGS
                     RSLDSLFLRLKSQNIPVEKVIKTVESLMIDLVLTAHPTEVTRRSLGHKHVEINKCLNL
                     LEHDDLTEAESYKIKRRLMQLIALAWHTNEIRTQRPTPVDEAKGGMAVIENSLWKAVP
                     EFCRQLNFYLEKHFGVQYPVNLAPVRFSSWMGGDRDGNPFVTAETTRRVLRMNRWKAT
                     ELFLQDIKNLAEELSITQCTPAFRAKYGNHLEPYRVLLKVLRQKLINTEIYYAELLEG
                     KISTIDESDILTSDQQLWEPLFDCYESLQACGMRIIANGALLDCLRRINCFGLGLSRL
                     DIRQESTRHEMAIAEITRYIGLGDYAHWTEDDKQAFLVRELSSRRPLIPTNWTPSPET
                     KEVLDTCKIVAEQPEGVISAYVISMAREASDVLSVHLLLKEAGCKFTIPVAPLFETLG
                     DLDHSEKVMTDLFNIGWYRGVINNYQMIMIGYSDSAKDAGMLAAGWAQYRAQEALVNL
                     CEKYNIELTLFHGRGGTIGRGGAPAHAALLSQPPRSLKNGLRVTEQGEMIRFKLGLPA
                     VAVESLELYASAILEANIFPPPEPKQQWRDVLAKASDISYQIYRDMVRGELDFVPYFR
                     SATPEQELSRLPLGSRPAKRNPKGGVDSLRAIPWIFAWMQNRLMLPAWLGAGQALQQL
                     IDEGNETLLKEMCNEWPFFSTRIGMLEMVFSKADIWLAEYYDQRLVEPKLHRLGNMLR
                     TQLQADLKTVLALAHDGQLMADLPWIAESIALRNIYTDPLNLLQVELLDRIRKQGENA
                     DPVLEQALMITITGIAAGMRNTG"
     gene            11310..11747
                     /locus_tag="PWX02_001252"
     CDS             11310..11747
                     /locus_tag="PWX02_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL1729270.1"
                     /translation="MKSFIEDGGNRMKQIYALGLAFIVASVSLLSTKVQANQAYQTTK
                     QGLQYKELKAGSGKKPTLNDEVEVRFTSYNDKGEVFEGTFNQVPVILPIREMFSGLQQ
                     GLLLMPVGSVYEFNIPAHLGYQEEGSRAKKAALYRIELLRINP"
     gene            complement(11906..>12719)
                     /locus_tag="PWX02_001253"
     CDS             complement(11906..>12719)
                     /locus_tag="PWX02_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDL1729271.1"
                     /translation="YAQNAIEAAPIVMLHSRYNKTINLLINNQIFTLQPKESELSPIS
                     LITDFSITKFQQLSFQPNQPYRLNLHYSNDAIFSSKLTACTSFSPNNVVWYANLAQKI
                     LQKSNAKGLNLLLQNSINDILLRAFKQYLVQAEDLYQLGELEKSTEYFTKLIGLGIGL
                     TPSGDDFLCGFIATFQYFTQTQTAFYQTLTQKLRQHLDNTNAISRRFLDCALQQQFSA
                     PILSFFNHTQQRQEDFCLLAQRFENIGHSSGMDTLFGIYHACKWLLKKSIIT"
BASE COUNT         3753 a         2397 c         2776 g         3793 t
ORIGIN      
        1 ccttcaatat attcattaaa gtgattaata actagatctg atggttctaa aatttccatc
       61 tcttttatca tttttgaatt gactgccgtt gcgattgatg atgtacttcc tagattatta
      121 aaatcaaaat tttttaagta gcagtataaa aactctgagc ttagatttga tgagctagga
      181 attttaaaat gagcaatagc ttcgttagta gttgtttctt ttgtcgtaat tgaaactcta
      241 ccaacggtta atttaaaact caaaattaca gtattttcag gaattctttt aatattaaat
      301 ttatctacgg cttcaacttt gaggtattcg ctactttccg taataaacaa tccctgattt
      361 cccatatctt taatagaaat ccattctgta tcatttgcat tatcagaaaa ccattcactc
      421 tcttttcttg gtggggtttt cccaatgcca acatcaaata aattgtctgc ttgattgaat
      481 ttccagccaa taggtaaccc ttcatctcca aactcacttg ggaaggcatc ggcgatttcc
      541 caaagctctt ggtattcggt ttggcttagg tcttcaatcg ctttgcctga aaagaccgcc
      601 attgctgata aattggcttc atcgctggtt ttgccgtcca aaatcgcttg ggctttggct
      661 cgtacagggt caaaatccac aaaccaactt ttaaaaatcg cctgagcaat ggcttctaag
      721 gtttggttgg tttgggtgtt gagttggatt ttttcttcca atatcattaa atattgagca
      781 attagttttt gggtttgcaa gtctggcttc tcaaactcga aatctagtat tttgctaggg
      841 gaagtatgat gaacagttgc accagtagct gtacttaata tttgatgtct gtattctttc
      901 gtacgcatca aaaaatacaa atactcttta tctagttcgt tatctaaaaa ttcaaccaag
      961 cctatccttt ggttatgcaa catttttttg cctgaaatat ttggcactaa tgctgggtat
     1021 cccaatgtat cggcttgctt gcttaaatcc gtcattgtta caattaaatc atttggctgt
     1081 aaaatgtatt tttcaggtat ttctcctgta taatatttga acttatctga tttaaaaccg
     1141 ccaccaatag aaaaattaac tggcgtaatc aaacagttgg cattttcttc tgttgtgatg
     1201 aattcacctt taaatgcaaa tccgtgttta atggaaataa aatcggatag tttactcatc
     1261 ttaaaacccc aaccctttta aatttgccac aatcttttct tccaatgctc gcccttgtct
     1321 aaattgttca tttaaaagtg cggttagttc ttgcattttt tgagcaaacg gtacgccgtc
     1381 atcttcttcg actgccgctc ctacatatcg cccagccgtt agcacaaagt catttttggc
     1441 gatttcgtcc agcgtggcac aataacaaaa cgcagggata ttttcgtagc cgtcctgttt
     1501 ttgccagttg tggtaggtgt cgctgatttt ggcaatgtca tcagcggtaa aatcacgcaa
     1561 gacacggtct ttcatatagc cgatttgggt agcgttgata aacagcacct ctttttttct
     1621 ggctttggct ttattgataa tccaaatgca ggcagggatt tgggtgttgg tgaatagctg
     1681 attgggcagg gcaatcatcg cttcgacaag gtcggcttgc acgatgtttt tgcgaatgtc
     1741 gccttcgcct gaggtttggc tactcattga gccgttggcg agcaggagtg ccattttgcc
     1801 tttgggggag aggtggtaaa tcatatgttg cagccacgca aagttggcgt tgccttgcgg
     1861 cggtgtgccg tatgcccagc gtgggtcgtt tgctaggctt tcactccacc acgctttttg
     1921 gttaaagggg ggatttgcca tcacaacatc catttttttg tcgatgtgca aagggtttag
     1981 tagggtatct tcgggcttgt cgccaaagtc aaagggaatg ccacgaatcg ccatattcat
     2041 caccgccagt ttgcgggtgt tggggttgga ttcttgccca taaaccgaaa tggcgttgcg
     2101 gttgccttga tgtgccagaa taaagcggtc agcctgcaca aaaaatccgc cgctgcccat
     2161 tgcagggtca taaatccgcc ctgaataggg tttgagcatt tcaacaatca gggtaacaat
     2221 ggatttgggc gtgaagtatt gaccgccttt tttgccttca gctagggcaa attgtccaag
     2281 aaagtattca tagacgtgcc ctaaaatgtc tttggcttgc agatgcacag gttcgccatt
     2341 gtgcataggc tgggtgaaat tggtgcgtga gaacaggtca attaagcctg tgagcatttc
     2401 atcaggcacg ccaaagccgg caatgcgttg gagtacgcct ttgagtttgg ggttttcccg
     2461 ttcgatggct tcaaaggcat catcaatcaa gcggctgaca cctttaaatt tgtctcccca
     2521 aggcaattca tcgccaagat tgagtttgct taatgatttg atgttgtccc agcgggcttg
     2581 ctccggcacc caaaagatgt tttcggcagc gtagtaatct ctctgttcca actcttcggc
     2641 aagaatttgg ctaaattctt gttcgtcaaa tagtgcaggg tcaagataga gttcgctttc
     2701 gggggtggta agctgggttt ttagcttggc ttggaaatcg gtaaagctgt cggagatgta
     2761 ttttaggaag ataaagccaa gaacgatgtg tttgtagttg gcggcatcga ggtttttacg
     2821 cagcttgtcg gcggctgtcc agagggtttg gtcgagatcg tttaaaaagg cttgactgac
     2881 cgtattgtca agagcaggtc gtgcagtgtt atttggcata gtggttcctt ttggatgtat
     2941 caatactaaa atgacctgta ttttagcaga tttacggata atcgaggaac tgataacatt
     3001 taaaaattag cgttttgcaa aattttccaa aaaaagaccg cttgttaggc tataatagcc
     3061 gtaatagagg agaaggtgta tggaatatca atttacccat agtattcacg gggtggtggc
     3121 aaaatgttca atggatcacg aagcatttgc ccgctggttg aatacggaaa ttacgggcaa
     3181 tccgaaagag ctagaagcta tttttgttga aattgaaaaa tgtagagtcg cttttcctaa
     3241 tcattatgaa tgtatatttg aaggtcgtga gtacagcctc tattttgatt atgatgaggt
     3301 gatggtaaaa gcgaataatt tagataccgc ttttgacgag aatgaaatgg aagatggctt
     3361 tcaattttat aatgaggaaa gcattgcttt ttgtggctta gatgattttg aaaaactgtt
     3421 aaaagcctac cagtactttg tgcaaactta tcattgatgg gaatgctgaa ttaaaacgat
     3481 actattaagg gaaccgaatt ccctgtagaa tagtgattta ttaaaatttt attaatcgat
     3541 taaagaatta tgagccgtac taaaaaaaca cgccgcatta ctgatattat gccagcaaga
     3601 aaagccgata aaccagcgaa gccaacacct aaattaggtg gcggcaaaaa attgaaactc
     3661 actcgttatg agttagatgt gttagcccgt gaggaaaaac gtaagcgtaa acataaaggc
     3721 ttgccgacag gctcacgcaa tgctgatccg gcagagcaga aaaaagcagt ggtgaaagag
     3781 gtgaaagatc cgcgtatcgg tagccgtaaa aaagtgccgt taatggtgga atttgtgaac
     3841 cagccggaaa aagggcggac gattaaggct gtgccggttg agccaatcaa accaacgctt
     3901 tcaccggaac aagagctgga gcagttagaa aataacgaat gtttaaatca gttgttagat
     3961 gatttggaag cgggcaaaac cttatccgca gaagatcaga aatttatgaa tgagtgttta
     4021 gatcgcattg atgaattaat gactgagctt ggcatcgaat atgaagatga agagggcgat
     4081 aatggtgatg cgttattacg ccaattcgaa acgatggata ttaataaatt ccgctaggta
     4141 gccgattatg atgctaagaa tattcctgat ggtgcttgcc gctttgattt taatttcgct
     4201 gggcgggtat gcactgcact taatgctgaa agtgcgagct cagaaaaagc acgaacaagc
     4261 actgattgaa caggcaaagc aagcacaaaa agatcgttat attcggattt tagacagcat
     4321 tgaagttatt gctcgggcaa tgatttccga acagtgcgat ttttcggaag gcgttttacg
     4381 tttaaaaccg ctattagatg ttctcggcag aaaattaagc gactatacgg cgatgtgggc
     4441 gttgtatggc gtggtggaaa atatgccgat tttagatgaa cgcaaaaatt taaaacgtaa
     4501 cgaaagaatg aagttggatt tagagcggga agcaaaagaa gcggagcttg aatcagacat
     4561 caaaaatgag tgttaccaat tattaaaaga tattgaagaa atgagaaagg caatctaatg
     4621 tcagaaatta tttgggattt agcccttatt caaaaatata accattcagg gcctcgttat
     4681 acctcttacc caacagcgtt ggagtttaac gaaaattaca ccaatgaaga ttttattcgt
     4741 gctgcagctc gttaccctga gcgtccgctt tcgctttatg tgcatattcc gttttgccat
     4801 aaactttgct atttctgtgg ctgtaataaa gtgattactc gtcatcgcca taaagttgaa
     4861 atttatttag attatttaga gcgtgaaatt aaaactcgcg cacctctgtt tcaaaatcgt
     4921 ttagtgaccc aaatacactg gggcggcggt acgccgactt atttagacga agatcaatcg
     4981 gcacgtttaa tgcaaatgct acgagccaat ttggatgtga gcgatgatgc ggaaatcagc
     5041 attgaaatgg atccgcgtga aatcgaatta agtatgttgg atcacctgcg tgctatcggt
     5101 tttaaccgtc tgagtatggg tattcaggat ttcgataaag aagtgcaacg tttggtgaat
     5161 cgtgagcaag atgaagagtt tatttttgcc ttgatgaaac gggcaaaaga acttggtttt
     5221 gtttcaacta atgtggattt gatttacggc ttaccaaaac agaatgtgga aagctttatg
     5281 tacaccttgc aacgtgtagt agagttaaac cctgatcgta tgagcgtatt taactatgct
     5341 cacttgccaa gccgttttgc tgcacaagtg aaaattaaag aagagttgtt gccggctccg
     5401 gaaactaaat tgaccatttt gcaaaattcc atcgaatttt taggcaaaaa tggctataag
     5461 tttatcggta tggatcactt tgccaaacct gatgacgagt tagccattgc acaagaaaaa
     5521 ggcattttac accgtaactt ccaaggctat accactcaag aagagtgtga tttattggga
     5581 atgggcgtat cggcaatcag cttactcggc gatacttacg ctcaaaaccg caaagaatta
     5641 caacaatact atgctgatgt tgaaaccaac ggcattgcgt tgcataaagg cattgcttta
     5701 agccaagatg attgcattcg ccgtgatgtg ataaaagcct tgatttgtaa tttcaaatta
     5761 gattttgcta ctatcgaaaa gcaatatggc attgatttta aacgctattt tgctgaagat
     5821 ttagctttct tagcgccgtt ggcggaagat gaattattaa cgatgaatga cgacagcatt
     5881 gtggtttctc cacgagggcg tttattgatc cgtaatattt gcttatgttt tgatgtttat
     5941 tcaagacaat tagcgagaag acagcagttt tcccgtatta tttaatgagc aagcggtcga
     6001 ttttggtaaa aaatttgcaa aaacagaccg cttgttcccg gcttataaag gaaaaatgat
     6061 gaaacagcgt tatagcttac tttctcttgc tgtgatggca gcttgttata gcaacagtgc
     6121 cgcagctgat ttaaagcaac aatgtttact tggcgtgccg cattttcagg gcgaagtggt
     6181 agaaggtgat caacttagaa tgcctgttac cattgagtca gacagtgcgg tgattaacca
     6241 accgcgtgat gccacctaca ccggtgatgt gaccatcaag caaggcaacc gctcgatttg
     6301 ggcagagcag gtgcgggttg agcaagatgg cgaaaatgcc cgtatggcgt atttgcaggg
     6361 taattttaac taccaagata atttgattca ggcaaccggt gcggatgcga ctattaattt
     6421 gctatctaaa gatgcgaatt taactaacgc caactaccaa ttagtcggca gacaagggcg
     6481 tggcacggca gaatccggcg agtttggcaa cagcaaacgt acgttgaaaa acgccagttt
     6541 cacttcgtgc ttaccaaatg ataattcgtg gcaaattgaa gcgaatgaaa tgattcagca
     6601 cgttgatgaa gaatatgctg aaatgtggca cgcccgtttt aaagtgctgg gaatgccggt
     6661 attttattcg ccttacttac aatttccgat tggcgaccgc cgccgtagtg ggttattaat
     6721 tccaagtttt gagcgttcaa gcaaaaacgg ctggacatat tcgcaaccgt tttattggaa
     6781 tatcgcaccg aatatggact cgaccatcac tccaacttac tattcacgcc gtggttggca
     6841 aatcagcccg gaatttcgtt atttaacgaa aatcgggcaa ggcttagtgg caaccgaata
     6901 tatggcgaaa gatcgtttaa gcgagtacca accgggaaat gatgatcgta aacgctattt
     6961 attccactgg cgtcacgcaa tgagtttttt aaccgattgg cgtttctcgg tcgattacac
     7021 caaagtgagc gacagacgtt atttctctga ttttgattcg gattatggta gcagtactga
     7081 tggctatgcg acgcagaatt ttaaattagg ctactatcag ccgaattata atatctcaat
     7141 ttcaggtaaa aaattccaaa ccttcgatga gttggatatc gggccttatc gcgttctgcc
     7201 acaaattgat tttaactatt ataaaaacga tctaattaaa ggcggagatt tcaaattctt
     7261 tgcccaagca gcccgttttg aaaatgatag taaattaatg ccaaaagcgt ggcgtttcca
     7321 cgctgagcca acactgaatt tcccattagc gaaccgctat ggtagcttaa atttagaaac
     7381 caagctctat gcaacctact atcaacaaac gaaaggtagt gagagtacgg aagaaattaa
     7441 gaaaagtgta agtcgtgtac tgccacagat taagctcgat tttaaaaccg tgttggaatc
     7501 cgataaacag cttttctctg gctatactca aacgcttgag cctcgggtgc aatatattta
     7561 tcgcccatat aaggatcaaa gcgatattgg ctcgaaagca catcgaagtg tgagcttagg
     7621 ctatgactct gcattattgc aaaacgacta tttctcactg tttaatgaca gacgttatag
     7681 cggtttagat cgtatttcgt cagcaaattt agtcactgca ggtggcacaa cccgtttctt
     7741 taaagaggca accggtgaag aggtgtttaa tttcagctta ggacaaactt attatataag
     7801 cccgtctaaa attgatgata cctcagataa cagcactaaa ggtcatacga cttcatgggc
     7861 agcggaatct aactggaaat tccataagaa ttggaattgg catggtagct atcaatatga
     7921 tactcgttta aaccaaacgg cgttagcgaa tatgtcattg caatacaaac cacagtcaga
     7981 caaattgatt cagcttaatt accgctatgc aagtaaagat tacatcaatc aaaacttgaa
     8041 cacgaataag tatgggcaat ccattaaaca agtgggcggt gtgctaggtt gggaattaac
     8101 cgataaagtg tcggtaatga ccagttatta ccacgacctt gcattgaaaa aaccggtgga
     8161 agggcaaatt tcgcttaact acaacacttg ctgttgggcg gcaaatgtgt atgtggctcg
     8221 ccgcttagtt tcaacgccgg taggttcatc tgatacgatc aatgatttct actatgacaa
     8281 taaattcggc attaactttg agctacgctt tggcacaaac tacagcaatg gtgtaacccg
     8341 tatgctaaaa cgaggtatta ttccttacac cgagccgtat ggcattaatt aacaaagtaa
     8401 aaacaaataa gtaaaaatac aaatcggggc gaaaaatatt tgccccgatt tttttatatt
     8461 tggctgataa gcggttaaat tctgttggaa ttttgcaaat cttccgctaa attcaaccgc
     8521 ttgccatcga aacttaacct gtatttcgca tacctgcggc aatacctgtg atggtgatca
     8581 tcaatgcttg ctctagtaca ggatcagcat tttcaccttg tttgcgaatc ctatctaaca
     8641 attctacctg aagtagattg agcggatcgg tataaatatt gcgtagtgca atcgattctg
     8701 cgatccaagg taaatcggcc attaattgtc catcgtgagc cagtgcaagt acggttttaa
     8761 gatcagcctg tagttgcgta cgcaacatat tgcctaaacg gtgtagtttt ggctcgacta
     8821 ggcgttggtc gtaatattcc gccagccaaa tatcagcttt actaaatacc atttccagca
     8881 ttccgatacg ggtcgagaaa aacggccatt cgttacacat ttcttttagt aaggtttcat
     8941 taccttcatc aatcaactgt tgcaatgctt gccctgcacc taaccaagca ggtagcatta
     9001 agcggttttg catccaagcg aaaatccaag gaattgcacg caaactatcc actccgcctt
     9061 ttggattacg ttttgcaggg cgtgagccaa gcggcagacg ggaaagttct tgctctggtg
     9121 tagcagagcg gaaatacggt acgaagtcta gctcgccacg caccatatca cggtaaattt
     9181 ggtaagaaat atcggaggct tttgctagca catctcgcca ttgctgcttt ggctccggtg
     9241 gtgggaaaat attcgcttct aaaatcgcac tggcatagag ttctaaactt tcaactgcca
     9301 cggcaggtaa gcctaattta aagcggatca tttctccttg ttcggtcact cgtaagccat
     9361 ttttgagtga gcgaggtggc tgagaaagta aggctgcgtg agccggtgcg ccaccacgcc
     9421 cgatagtacc accacgaccg tggaataagg ttaattcaat attatatttt tcacataaat
     9481 taaccagagc ttcttgcgcc cgatattgcg cccagccggc agcaagcatt ccggcatctt
     9541 tggcggaatc agagtagccg atcataatca tttggtaatt gttgatgacg ccacgatacc
     9601 agccgatatt aaataaatcg gtcatcactt tttcactatg atctaaatcg cctagcgttt
     9661 caaataatgg ggcaaccgga atagtaaatt tgcaccccgc ctctttgagt aaaaggtgaa
     9721 ccgagagtac atcagaggcc tctcttgcca ttgaaatgac ataggctgaa atgacacctt
     9781 caggttgctc ggcaacaatt ttacaagtgt ctaacacttc tttagtttca gggcttggag
     9841 tccagttggt tggaatcaat gggcggcgag aactgagttc acgcactaag aatgcctgtt
     9901 tatcatcttc cgtccagtga gcataatcgc ctaagccgat atagcgggta atttctgcaa
     9961 tcgccatttc gtggcgggta ctttcttggc gaatatctaa gcgtgataaa cctaaaccaa
    10021 aacagtttat acgacgtaag caatctagta atgcaccatt tgcaataatt cgcataccac
    10081 aggcttgtaa tgactcatag caatcaaaaa gcggttccca caattgctga tctgaagtta
    10141 aaatgtcact ttcatcaata gttgaaattt taccttccaa taattccgca tagtaaattt
    10201 cggtattaat taatttctgg cgtaatactt ttagtaatac gcgatatggc tctaaatggt
    10261 tgccatattt agcacgaaat gcaggggtac actgtgtaat cgataattct tcagcaagat
    10321 ttttaatatc ttgtaagaac aattccgtgg ctttccaacg gttcattctt aatacacggc
    10381 gagttgtttc tgccgtgacg aacgggttac cgtcacggtc tccgcccatc caagaagaaa
    10441 aacggacagg ggctaaattt accggatatt gcacaccaaa gtgtttttct aaatagaagt
    10501 taagctgacg gcaaaattcc ggcacggctt tccataaact attttcaatg actgccatac
    10561 cgcctttggc ttcatcaaca ggggttgggc gttgggttct gatttcatta gtatgccaag
    10621 caagggcaat cagttgcatt aaacggcgct tgattttata gctttctgcc tcagttaaat
    10681 catcatgttc caacaaattc aaacatttat tgatttcaac gtgtttatgc cccagcgaac
    10741 ggcgggttac ttccgttggg tgagctgtta gcactaaatc aatcattagg ctttccacgg
    10801 ttttaatcac tttttcaacg gggatatttt gtgattttag acgaaggaac agagagtcta
    10861 atgagcgact accaagtgat tcatcaatat gatggcgaga aacagtttga tgttgttcag
    10921 caatatttgt tagatttaga aaatggctaa atgcacgtgc aacgggtaag acatcatcgt
    10981 tagaaatatt ggctaattta tctaatagct gttcacgggc gttttcatcg ccggcacgag
    11041 aattgcgaga taacacacga atgctttcga tgagttctag aatgtaattt ccttgcgcat
    11101 cacgaatcgt ttctcccaaa aattcaccta acatatggat attatgctga attgttgaat
    11161 attgctggtt cataaaaaat tcctatggta aacataaatt aaattaggtc taaatatgcc
    11221 gaaaatcgct gaaaatagca aatagattta atttaatttt tgaattaaat gataaaacgc
    11281 aaaaaatagc tgtctatggt agggataata tgaaatcttt tatagaagat gggggaaata
    11341 gaatgaagca gatttatgct ttaggtttgg cattcattgt cgctagtgta agtttattat
    11401 ctactaaagt acaggcaaat caagcgtatc aaactactaa gcaaggcttg caatataagg
    11461 agcttaaagc aggcagcggc aaaaaaccaa cactaaatga tgaagtggag gtgcgtttta
    11521 cttcttataa tgataaaggg gaagtgtttg aagggacgtt taatcaagtg ccagtaattt
    11581 tgcctatcag agaaatgttt tccggcttac agcaaggatt attgcttatg ccggtaggct
    11641 ctgtatatga gtttaatatt ccggctcacc taggttacca agaagagggc agtagagcta
    11701 agaaagctgc gttatatcgg atagaactac ttagaattaa tccttaaact cagaaaacct
    11761 atatagaaaa agcattttac aaatttttcc ctaaattcaa ccgcttgcat attactacaa
    11821 gcggtatttt tctgcctaaa taacgctagt tgctcgtcac atttttaaat tcaactgctt
    11881 tattacgtca aaaagccaaa cacattcagg tgatgatgga tttttttaat agccatttac
    11941 aagcatggta tatcccgaaa agtgtatcca ttccggaaga atgccctata ttttcaaaac
    12001 gttgtgctaa taggcagaaa tcctcttgac gttgttgtgt gtgattaaag aagctcaaaa
    12061 taggggcgga aaattgttgt tgcaatgcac aatctaaaaa acggcgacta atcgcattag
    12121 tattatctaa atgttgtcgg agtttttggg ttagtgtttg gtaaaatgct gtctgagttt
    12181 gagtaaaata ttgaaatgtg gcaataaaac cgcatagaaa atcatcgcca cttggcgtga
    12241 gaccaatacc aagcccaata agtttggtga aatattccgt tgatttttct aattcgccta
    12301 gttgataaag atcctcagct tgaactaaat attgcttgaa tgcacggagt agtatatcgt
    12361 taatactgtt ttgtaataat aaattcagtc ctttggcatt cgacttttgc aagatttttt
    12421 gcgcaagatt tgcataccaa accacattgt ttggtgaaaa agaagtgcaa gcggttaatt
    12481 ttgaagaaaa aattgcatca tttgaataat gtaaattgag ccgataaggt tggttaggtt
    12541 ggaatgagag ttgttgaaat tttgttattg agaaatctgt gattagactg atcggtgaaa
    12601 gctcactttc tttcggctgt aatgtaaaaa tctgattatt aatcaataaa ttaatagttt
    12661 tattgtaacg agagtgaagc attacaatag gtgcagcttc tattgcattt tgagcgtac
//
LOCUS       DANJWC010000062        12683 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.062, whole genome shotgun sequence.
ACCESSION   DANJWC010000062 DANJWC010000000
VERSION     DANJWC010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12683
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.062"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..786
                     /locus_tag="PWX02_001254"
     CDS             <1..786
                     /locus_tag="PWX02_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729272.1"
                     /translation="ITKFVKVDRDTVFEFFNISLHNPRKNGKNRICSPREFREYRKLK
                     KPDLFNDNPAFFINKGQKGIPQGSPISALLSNIYMLEFDVLVKEKIMECKGSYFRYCD
                     DILCIIPNEYEEFILDYITGEIKSKLKLEINKDKTEVVKFQYCNRTKKIINEKKLQYL
                     GFILHNNSISIRSSAFTRYSNKMKRGVSLAKQTQGKYNRIRIRRGVAIKGIYKRKLFS
                     KYSHFGKSNFISYGKRAYSSMDSKVIKNQLKPLWYRLKKEIYL"
     gene            complement(1038..2318)
                     /gene="hemL"
                     /locus_tag="PWX02_001255"
     CDS             complement(1038..2318)
                     /gene="hemL"
                     /locus_tag="PWX02_001255"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDL1729273.1"
                     /translation="MSISEQLFEQAQKVIPGGVNSPVRAFKGVGGTPVFIEKAEGAYI
                     TDSDGKQYIDYVGSWGPMVLGHNHPAIIDAVLKAVPKGLSFGAPTAAEITLAELVCKL
                     VPSIEMVRMVSSGTEATMSAIRLARGYTNRDKIIKFEGCYHGHSDSLLVKAGSGALTL
                     GQPSGPGVPADFAKHTLTCTYNDLDSVKAAFEQYPNEIACLIVEPVAGNMNCIPPQEG
                     FLQGLRDLCTQYGAVFIIDEVMTGFRVALGGAQSYYGVTPDLTTLGKIIGGGMPVGAF
                     GGKKEIMEYIAPTGPVYQAGTLSGNPIAMAAGLACLTELSKAGNETILAEKTKTLAEG
                     FKALADKHNVPLTVQYVGGMFGLFFTEQKAVNNFQEVMKCDAAKFNRFFHLMLEQGVY
                     LAPSAFEAGFMSLAHSDEDIARTLEAADKAFAQL"
     gene            2460..3524
                     /gene="wecA"
                     /locus_tag="PWX02_001256"
     CDS             2460..3524
                     /gene="wecA"
                     /locus_tag="PWX02_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HDL1729274.1"
                     /translation="MWLTFLTVFIVSFLALIVMRPVAEFVGLVDKPNFRKRHQGLIPL
                     IGGIALFIGNLTFYFMQWQDMRLPELYLTAVTILLIIGVLDDRFDISPLLRAGIQAAL
                     AGAMIYSGLSLSSLGQIIAPFSIELGWLGIVFTVFITIGVINAFNMVDGIDGLLAGLS
                     SVSFAGLGTLMFINGEPSLGYWCFALIFVLLPYAMFNLSLFGAKWKVFMGDSGSMLIG
                     FTIIWILLLSTQGQGSPISPITGLWLIAVPLIDMVAVIIRRLKKGKSPFKPDRLHLHH
                     LMMRAGLTSRQALLVITLWAGFCSTIGVLGEVYYWNQWVMMLMFIGLFFLYAYSITHA
                     WRVTRFVRRLKRRAKRSKQL"
     gene            3535..4347
                     /locus_tag="PWX02_001257"
     CDS             3535..4347
                     /locus_tag="PWX02_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS chain length-determining protein"
                     /protein_id="HDL1729275.1"
                     /translation="MKTLFYRLLVLILFAAIGAGAGYGLSYTQPEKWKVTAQFEQPTI
                     PDLGNYYSLLSTYHFLNSNSQEANSQTAQQQASEQVYAEFKRNLTSADLLQAYLLQTE
                     AVKLKAQVAKKSPAVVAAELVEQFRFEKDMATSADNVSLIAENAEEAQKLLADFIPLV
                     NTQARDKLNADLIAKWKVLFQQIKTASELNINGKQGSQDWAAKLQMMRSVQPLDNQLT
                     AYRLVKSPSVPLKSESPDPPILADDWGIKWLTAGLYAQFCGESESSTCGTLI"
     gene            4388..5635
                     /gene="wecC"
                     /locus_tag="PWX02_001258"
     CDS             4388..5635
                     /gene="wecC"
                     /locus_tag="PWX02_001258"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDL1729276.1"
                     /translation="MMSKTEFNTICILGLGYIGLPTSVVFANAGKQVIGVDINPQVVA
                     SLNQGNIHIIEPDLQTAFTQAVKKGNFFATAKPQPADAFIIAVPTPLCGKQPDLTYIQ
                     NAAAMIAPCLRKGNLVVLESTSPVGTTENLAEWLSQARPDLAFPADVSIAYCPERVLP
                     GNIMTELVENDRIIGGLSEQCSQQAVDLYQIFAKGRCVTTTARTAEMCKLVENSFRDV
                     NIAFANEISMICDALKINVWELIELANLHPRVNILQPGAGVGGHCLAVDPWFIVSTSP
                     QQARLIRTAREVNDSKPQWVIEQVKTSVADCAMEQDCKPSELTIACLGLAFKPNIDDL
                     RESPALAITRQLAAWHQGSLLAVEPNIQQLAGEKITLVDLNTALSQADILVLLVDHTS
                     FKQISREQIRQRHIIDCRGVWVR"
     gene            5632..6276
                     /gene="rffC"
                     /locus_tag="PWX02_001259"
                     /gene_synonym="wecD"
     CDS             5632..6276
                     /gene="rffC"
                     /locus_tag="PWX02_001259"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HDL1729277.1"
                     /translation="MKPDQWLSDFFGREIVQVKVAADDFRQIARLQKQGFVLVEGELE
                     FELALATFSENMTACRPADMADLPDLNLLFGSAFPDSRFRSPYFSREENQRFYRTWIE
                     NAVKGKFDDIGLVQCNADGQIQGAVTVRLLAQQQAKIGLLAVKPDCRQQGIGSELLNA
                     AIAWAKQQNAKKLMITTQLSNQKAIRFYQAFGASIKAVYYWFYPALEKYDDEQS"
     gene            6263..7408
                     /gene="rffA"
                     /locus_tag="PWX02_001260"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             6263..7408
                     /gene="rffA"
                     /locus_tag="PWX02_001260"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HDL1729278.1"
                     /translation="MNNPDFIPFNQPPVVGAEQAYIEQAMATGQLSGGGKFTRLCEQW
                     LENLARSKVLLTPSCTAALEMAAILLDIQPDDEVIMPSYTFVSTANAFALRGAKIVFV
                     DIRPDTMNIDERKIEQAITPKTKVIVPVHYAGIACDMDSIMATAEKYGLYVVEDAAQA
                     VNSFYKGRALGSIGHIGCYSFHETKNYTAGGEGGAIVINEANLLEKAEIIREKGTNRS
                     QFFRGEVDKYRWQDIGSSFLMSEIQAAYLYAQLIEADKINQQRLTLWHNYYQAFLPLA
                     QTAKCELPRIGEYCRHNGHLFYLKLSDLKQRTAFIDFMKAQNILTVFHYVPLYSSPAG
                     QKLGRLAGDDRFTTLESERLVRLPLFYNLSKQEQERVIQAVLAFFNQ"
     gene            complement(7453..8874)
                     /gene="glnA"
                     /locus_tag="PWX02_001261"
     CDS             complement(7453..8874)
                     /gene="glnA"
                     /locus_tag="PWX02_001261"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDL1729279.1"
                     /translation="MSNAIANVSKLIQDNDIKFVLLKFTDIKGKEHGVSLPVSLVAND
                     LEDLFEEGKMFDGSSVEGWKAINKADMLLMPIAETAVVDPFARIPTLSIRCSIYDPNT
                     MQSYDRDPRSIATRAENYLKSTGIADSVMFGPEPEFFLFDDVRFSTEMNNVSYRIDDV
                     EAAWNTNRKFEEGNKAYRPLKKGGYCAVAPIDTAHDIRSEMCLLMEEMGLVIEAHHHE
                     VATAGQNEIATRFNSLTLKADETQIYKYIVQNVAVEHGKTACFMPKPFAGDNGSGMHC
                     NMSLRKDGKNVFMGDKYAGLSETALYYIGGIIKHAKALNAFTNPTTNSYKRLVPGFEA
                     PVLLAYSASNRSASIRIPAVTSPKATRVEARFPDPMANPYLCFAALLMAGLDGVINKI
                     HPGDAMDKNLYDLPPEELKDIPTVASSLESALDALSNDFEFLTQGGVFTKDFIDAYIG
                     IRRKEVERVNMTPHPVEFELYYS"
     gene            9061..10131
                     /locus_tag="PWX02_001262"
     CDS             9061..10131
                     /locus_tag="PWX02_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HDL1729280.1"
                     /translation="MLPIYHILGSEIEHHNQQVLTFFQQELLPLLPDSEHFFYVVGKP
                     SLQTAFTHLRIKQFSSQKAIANALIAKKRQNPTACFLLHGQFNPWIWLAIALGRLPTT
                     NLIWHIWGADLYEMATSWKFKLFYPFRRVAQQKILQVWATRGDLDHLWRNMRARSEQD
                     HLIYFPTKLGDQIKISPTQNDRLTILLGNSGDPANELIQALSEIHQRLGNNVRIIIPM
                     GYPENNESYIRQVEAYSKRLFLAENLQILRKKLSFEEYVTLLSHCDLGYFNFKRQQGI
                     GTISLLISHNVPCVLHSANPFGLDLQAEKVPYLLAENLTASQIQTTKQQLKSIDKNRI
                     AFFAPGYTKLWQQRLIELMQNE"
     gene            10124..11395
                     /gene="wzyE"
                     /locus_tag="PWX02_001263"
     CDS             10124..11395
                     /gene="wzyE"
                     /locus_tag="PWX02_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HDL1729281.1"
                     /translation="MNNLIPLGGFYLVSVGLFFSLLYQLYQKSGISFHLLFSLLYFVT
                     FYAGFPFSLIMAFGFDYPLPELKTQWQVFTAAGVGYLIYYVVYSRLWSAVSQPTALVA
                     NFQAKTTACILAAVAVVALLYFLYLNGFLLFKLEKYSQLFAKDRVILSALKRFFYFLL
                     PALLIFCFLYKNKTAWWALLIVGGAFGILSYVAVGGTRANLALAVGFFLLIGLQQGYL
                     SLSKVIAVSLIGVVAMFFLALARYKLTLSGSEMIYTFLYLSRDTFSPWENLARVFSNE
                     VEYQGLMPIVRDFYVYIPQSLWQERPEIVWNTANYFTKVILGNQSGLAISPTLLGSFY
                     IMGGFSVMALGMGIVAAMIKALDNLFAYAKACNSALLQAYCFGNLFNLIVLVREGIDA
                     FVSRFCFFTLIFMACYLLAKILSMGKNNNVR"
     gene            11385..12107
                     /gene="rffM"
                     /locus_tag="PWX02_001264"
     CDS             11385..12107
                     /gene="rffM"
                     /locus_tag="PWX02_001264"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HDL1729282.1"
                     /translation="MFDKVIIRGIALQAVKNREIFANWLMNEKRIQFGRLMAINAEKV
                     ILSEKNPLLRRVIDEAEFNYADGISVVQSIRKKYPNYKIERIAGADLWETLMQRAGAL
                     NVPVFLVGSSADTLTKVEAKLAQWKVNIVGRQNGYFKAEQEQDVIEQIKASGAKFISV
                     AMGTPKQELFIQQLQQQYPQALYMGVGGTYDVFAGKVKRAPKIWQNLGLEWLYRLLKQ
                     PTRWQRQLNLLKYAYYYLTNQL"
     gene            12266..12342
                     /locus_tag="PWX02_001265"
     tRNA            12266..12342
                     /locus_tag="PWX02_001265"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12300..12302,aa:Arg,seq:ccg)
     gene            12372..12447
                     /locus_tag="PWX02_001266"
     tRNA            12372..12447
                     /locus_tag="PWX02_001266"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12405..12407,aa:His,seq:gtg)
     gene            12472..12548
                     /locus_tag="PWX02_001267"
     tRNA            12472..12548
                     /locus_tag="PWX02_001267"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12506..12508,aa:Pro,seq:tgg)
BASE COUNT         3491 a         2506 c         2902 g         3784 t
ORIGIN      
        1 attactaaat tcgtaaaagt agatagagat actgtatttg aattctttaa tatctccctt
       61 cataatccta ggaaaaatgg gaagaataga atttgtagcc ctcgggaatt tcgagaatat
      121 aggaaattaa agaaacctga cttatttaat gataatccag ctttttttat taataaaggc
      181 cagaaaggga ttcctcaagg ttctccaata agtgcattgt tatccaatat ctatatgtta
      241 gagtttgatg tgttagttaa agaaaagatt atggagtgca aagggagtta ttttcgttat
      301 tgtgatgaca ttctatgtat cattcctaat gaatacgaag aatttattct cgattacatc
      361 actggggaga taaaaagtaa attaaaacta gagataaata aggataaaac tgaggttgtt
      421 aaattccaat attgtaatag aacaaaaaaa ataattaatg aaaagaagtt acaatatctt
      481 ggttttatat tgcataataa ctctattagt attagatcat cggcatttac acgatattca
      541 aataaaatga aacgaggggt tagtctagct aagcaaactc agggtaaata taataggata
      601 agaataagac gaggggtagc tatcaaaggc atttataaaa gaaagctatt ttctaagtat
      661 tctcatttcg gaaaatctaa ttttatctct tatggaaaga gagcttattc atctatggat
      721 tccaaagtta taaagaacca acttaaacct ttatggtata gattaaagaa agaaatatat
      781 ttgtaatatt agaaagtgta agacttgtcc ttgcctgcaa gtgttggaag cccatatatt
      841 gctttatcaa tcctattccc tcgatagcgc ccgtctccag acgggtgctt gtttaagcat
      901 aatgaaagtt tgtataagca ctcgtcagag acgaacacca tcataattgc tatcgtaaga
      961 gaaatttgca ctacaagcgg tcaaattccc tcaaaaactt gcaaattatt gagtaaaccc
     1021 aaccgcttgt cattcttcta aagctgagca aatgccttat ccgcagcctc tagcgtacga
     1081 gcaatatctt catcactgtg agctaaagac ataaaccctg cctcgaatgc cgatggggct
     1141 aaatacacgc cttgttccag cattaagtgg aagaaacggt tgaatttggc ggcatcacat
     1201 ttcattactt cttggaagtt gtttactgct ttttgttcgg tgaagaataa gccgaacatt
     1261 ccgccgacat attgtaccgt taatggcaca ttgtgcttat ctgcaagagc tttaaagcct
     1321 tcagcgaggg ttttggtttt ttccgctaaa atggtttcat ttcctgcttt tgataattca
     1381 gttaagcatg ccaaacctgc agccattgca atcgggttgc ctgagagcgt gccggcttgg
     1441 taaaccgggc cagttggagc gatgtattcc ataatctctt ttttaccacc aaatgccccc
     1501 accggcattc cgccgccgat aattttgccg agtgtggtta aatccggtgt taccccatag
     1561 taagattgag ccccaccgag tgcgacacgg aagccggtca ttacttcatc aataataaat
     1621 accgcaccat attgtgtgca aagatcacgt aagccttgta aaaagccttc ttgtggcgga
     1681 atgcagttca tattgccggc aacaggctca acaattaaac aagcgatctc atttgggtat
     1741 tgctcaaatg ctgcttttac tgaatctaaa tcattatagg tgcaagtaag cgtatgcttg
     1801 gcgaaatcag cgggtacgcc cgggccgctt ggctgaccga gggttaaagc acctgaaccg
     1861 gcttttacta acagagaatc ggaatgcccg tggtagcagc cttcaaattt gatgatttta
     1921 tctcggttgg tatagccacg agccaaacga atggcagaca ttgttgcctc tgttccgctg
     1981 ctcaccattc tcaccatttc aatagaaggt actaatttgc acactaattc ggcaagtgtg
     2041 atttctgctg ccgttggggc accgaaactt aagccttttg gcacggcttt tagcaccgca
     2101 tcaataatcg ccgggtggtt atgcccgagt accattggcc cccaagagcc gacatagtcg
     2161 atatattgtt tgccgtcgct gtcggtaata taagcacctt cggctttttc aataaaaacc
     2221 ggtgtgccgc ccacgccttt aaatgcacgc acaggggagt tcacaccgcc cggaatcact
     2281 ttttgggctt gttcaaacag ttgttctgaa atagacatat caattccttt cttttgatgt
     2341 aaaaatttgc ctgttattgt actgaaaagc ccttgttttc gctaaaaaat ttacgatttt
     2401 gttgccttat gctattctgc attcctattt tatgactcat tttttgaggc aatccctgta
     2461 tgtggcttac ttttttaacc gtatttattg tttctttttt agccctgatt gtgatgcgtc
     2521 cggtggcgga atttgttggt ttggtagata agcccaactt ccgtaaacgc catcaggggt
     2581 tgattccgtt aatcggtggt attgcattat ttatcggcaa tctcactttc tattttatgc
     2641 agtggcaaga tatgcggctg ccggaactgt atctgacagc ggtaactatt ttgctgatta
     2701 tcggcgtgtt agatgaccga tttgacatca gcccattact gcgtgccggg attcaagcag
     2761 cactagctgg ggcgatgatt tacagtggct tgtcgctcag cagtttaggg caaattattg
     2821 ccccctttag tatagaatta ggttggctcg gcattgtctt taccgtgttt atcaccattg
     2881 gcgttattaa tgcgtttaat atggtagatg ggattgatgg cttattagca gggctttcca
     2941 gcgtgagttt tgccggtctt ggcactttaa tgttcattaa tggcgaacca agtttgggct
     3001 attggtgctt tgcccttatc tttgtgttgt tgccgtatgc aatgttcaat ttgagcctgt
     3061 ttggggcgaa atggaaagtg tttatgggcg actcaggcag tatgctgatc ggctttacca
     3121 ttatttggat tctgctgtta agcacccaag ggcaaggctc gccaattagc ccgattaccg
     3181 gtttgtggct gattgccgtg ccgttaattg atatggttgc ggtgattatc cgccgtttga
     3241 aaaaaggtaa aagtccgttc aaaccagacc gcttgcacct acaccattta atgatgcgag
     3301 cagggcttac ctctcgccaa gcattattgg tgattaccct ctgggcggga ttttgttcca
     3361 ccattggggt attaggcgag gtgtattatt ggaatcagtg ggtgatgatg ctaatgttta
     3421 tcgggctgtt tttcctatac gcctattcca ttacccacgc ttggcgtgta acccgttttg
     3481 ttcgccgtct aaaacgccgt gcaaaacgta gcaaacaact ttaaaggata tgttgtgaaa
     3541 accttatttt atcgactttt agtgctaatt ttatttgctg ctatcggggc aggtgccggt
     3601 tatgggctga gttatactca acctgaaaaa tggaaagtga ccgctcaatt tgagcaaccg
     3661 actattcccg atttaggtaa ttattactcg ttactttcca cctaccattt tttgaatagt
     3721 aatagccaag aagcgaatag ccaaacggca cagcaacagg caagtgagca ggtttatgcg
     3781 gaatttaaac gcaacctcac gtccgccgac ttgcttcaag cctatttgct acaaacagaa
     3841 gcggtgaaat taaaagctca ggttgcaaag aaatcaccgg cggtggtggc ggctgaatta
     3901 gttgaacaat tccgttttga aaaagatatg gcaacttcgg cagataatgt cagcttaatt
     3961 gcagaaaatg cggaagaggc tcaaaaatta ttagccgatt ttattccgtt ggtaaacacc
     4021 caagctcgag ataaattaaa cgccgattta attgcgaaat ggaaagtctt gttccagcaa
     4081 attaaaactg cttcggagtt gaatattaac ggtaagcaag gctcacaaga ttgggcagct
     4141 aaactgcaaa tgatgcgttc tgttcagccg ttagacaacc aacttacagc ttatcgcttg
     4201 gttaaatcgc ctagcgttcc acttaaatca gaatcgcctg atccgcctat tttggctgat
     4261 gattggggca ttaagtggct tactgctggg ctttatgctc agttttgcgg tgaaagcgaa
     4321 agctcaacct gcggaacttt aatctaaaat ggcacacaat ttagtgtgcc atttctcttt
     4381 gtaactgatg atgagtaaaa ctgaatttaa cactatttgt attctggggt taggctatat
     4441 cggcttgccg acctcagtcg tttttgccaa tgccggcaag caagtaattg gggtggatat
     4501 taatccgcaa gtggtggcaa gccttaatca aggcaacatt catattatcg agccggattt
     4561 acaaactgcc ttcacacaag cggtcaaaaa aggtaatttt tttgcaactg ccaaaccgca
     4621 accggccgat gcttttatca tcgccgtgcc aaccccactt tgcgggaagc agcccgatct
     4681 cacttatatt caaaatgccg ccgctatgat tgccccttgt ttgcgtaaag ggaatttggt
     4741 ggtgctggag tccacttcgc ctgtcggtac aacagaaaat ttagccgaat ggctaagcca
     4801 agctcgccct gatttagcct ttccagccga tgtgtctatt gcctactgcc ctgaacgtgt
     4861 tttgccgggt aatataatga cggaattggt tgaaaatgac cgcattatcg gcggattaag
     4921 cgaacaatgc agtcaacaag cggtcgattt ataccaaatt tttgcaaaag gacgttgtgt
     4981 caccaccaca gctcgcacag cggagatgtg caagttggtg gaaaacagct ttcgtgatgt
     5041 gaacattgcc tttgccaatg aaatttcaat gatttgtgat gcgttgaaga tcaatgtgtg
     5101 ggagctaatt gaattggcaa atcttcaccc gagagtaaat attctccagc ccggagcagg
     5161 ggtgggcgga cattgtttgg cggttgaccc gtggtttatc gtttccacat cgccccaaca
     5221 ggctcgcctt attcgcacgg cacgagaagt gaatgacagc aaaccgcaat gggtaatcga
     5281 gcaagtgaaa acatcggtcg cagactgtgc gatggagcaa gattgtaagc caagtgagct
     5341 aaccatcgct tgtttagggc tagctttcaa gccgaatatt gatgatttgc gggaaagccc
     5401 ggcattagcg attacccggc aacttgccgc ttggcatcag ggtagcctgt tggcggttga
     5461 gccaaatatt caacagctgg ctggcgaaaa aatcacctta gtcgatctca acaccgcact
     5521 ttcgcaggca gacattttag tcttgttagt ggatcacact tcatttaagc aaatttcaag
     5581 ggaacagatc aggcaacggc atattattga ttgtcgagga gtgtgggtaa gatgaagccg
     5641 gatcaatggc tgtccgattt tttcggtaga gaaattgtgc aagtaaaagt agcggctgat
     5701 gattttaggc aaatcgctag gttgcaaaaa caaggttttg tacttgtgga gggagaattg
     5761 gaatttgagc tggcacttgc aacattttct gaaaatatga ccgcttgtcg cccggcggat
     5821 atggcagatt tgcccgactt gaacttattg tttggttcgg catttcccga tagccggttt
     5881 cgttcgccct atttttcaag ggaagagaat caacgctttt accggacttg gattgaaaat
     5941 gcggtaaaag gcaagtttga tgatattggc ttggttcaat gcaatgctga cgggcaaatt
     6001 caaggggcgg taacggttcg cttacttgcc caacagcaag cgaaaatcgg attattggcg
     6061 gtaaagcctg attgccgaca acaaggtatt ggctcggaat tgttaaatgc tgcgatagcg
     6121 tgggcgaaac agcaaaatgc gaaaaaactg atgattacca ctcagctgag taaccagaaa
     6181 gcgattcgtt tttatcaagc gtttggtgca agcattaagg cggtgtatta ttggttttac
     6241 cctgctttgg aaaaatacga tgatgaacaa tcctgatttt attccgttta atcaaccccc
     6301 ggttgtgggg gcggagcaag cctatataga acaggcgatg gcaacagggc agctttcggg
     6361 cggtggtaaa ttcacccgct tatgtgagca gtggcttgaa aacctggccc gtagcaaagt
     6421 gttgcttacc ccttcttgca cggctgcgtt agagatggcg gcaatactgc tggatattca
     6481 gccggacgat gaagtgatta tgccgagcta tacttttgtc tctactgcaa atgcgtttgc
     6541 gttgcgtggg gcaaagattg tgtttgtgga tattcgtccg gatacaatga atattgatga
     6601 acgcaaaatt gagcaagcca ttaccccaaa aaccaaagtg attgtgccgg tgcattatgc
     6661 cggcattgct tgtgatatgg attcaataat ggcaacagcc gaaaaatacg ggctttatgt
     6721 ggtagaagat gcggcacagg cagtcaattc gttttataaa ggtagggcgt taggttccat
     6781 cgggcatatt ggttgctata gttttcacga aaccaaaaac tataccgctg gcggtgaagg
     6841 tggggcgatc gtgattaatg aggcgaattt gctggaaaaa gcggaaatca tcagagagaa
     6901 aggcactaac cgtagccaat tttttcgtgg tgaggtagat aaatatcgct ggcaggatat
     6961 tggttcaagt tttttaatgt cggaaattca agcggcttat ttgtatgctc agcttataga
     7021 ggcagataaa atcaaccaac agcgtttaac cctttggcac aattactatc aagccttttt
     7081 gccacttgcc caaacagcta agtgcgagct tcctcgcatt ggcgaatatt gtcgccataa
     7141 cgggcatttg ttctacttaa agctaagtga cttgaagcag agaactgctt ttattgattt
     7201 tatgaaggca cagaatatct taaccgtgtt tcattatgtg ccgttgtaca gcagtccggc
     7261 agggcagaaa ttaggtcgat tagcaggtga tgatcgcttt actaccttag aaagcgaacg
     7321 gttggtaaga ttgccactgt tttataattt gagtaagcag gagcaagagc gtgttattca
     7381 agcagtgctt gctttcttca atcaataaaa aacggctgag tgtttcagcc gtttttttgc
     7441 tatatgttgt tactatgaat agtaaagttc aaattctacc gggtgcggag tcatatttac
     7501 acgttccacc tctttgcgac gaataccgat ataagcatca ataaagtctt tcgtgaacac
     7561 gccaccttgt gttaagaatt caaaatcatt gcttaatgcg tcaagagcgg attctaaaga
     7621 cgaggcaacc gttggaatat cttttaactc ttccggcggc aaatcgtata agtttttatc
     7681 cattgcatcg cccgggtgga ttttgttgat cacaccgtct aagccagcca ttaacagggc
     7741 ggcaaagcaa aggtatgggt ttgccattgg atccgggaag cgtgcttcta cacgggtcgc
     7801 tttcggactg gtcacagccg gaatacggat agaggcagag cggttacttg cagaatatgc
     7861 caataacacc ggtgcttcaa aacccggcac taaacgttta taagagttcg tggttgggtt
     7921 agtaaaggca tttaatgctt ttgcgtgttt aatgataccg ccaatatagt agagggcagt
     7981 ttcagataag ccggcgtatt tatcgcccat aaacacattt ttgccgtctt tgcgtaatga
     8041 catattacag tgcattcccg agccgttgtc gccggcgaac ggttttggca taaagcaagc
     8101 ggtttttccg tgttctacgg caacattttg cacgatgtat ttatagattt gggtttcatc
     8161 ggcttttaag gtcaggctgt tgaatcgggt tgcgatctca ttttgtccgg ctgtcgcaac
     8221 ttcgtggtgg tgagcttcaa tcactaaacc catctcttcc atcaataaac acatttcaga
     8281 gcgaatatcg tgggcggtat caatcggtgc aaccgcacag tagccgcctt ttttcaacgg
     8341 acgataggct ttgttgcctt cttcaaattt acggttggta ttccaagccg cttctacatc
     8401 gtcaattcgg tatgacacat tgttcatttc ggtgctaaaa cgcacatcat cgaataagaa
     8461 aaattcaggc tcaggtccaa acattacgct atcggcgata ccggtggatt tcaagtagtt
     8521 ttcggcacgg gttgcaatag agcgagggtc acggtcataa gactgcattg tgtttgggtc
     8581 gtaaatggaa caacgaatag aaagggtagg aatacgggcg aaagggtcaa ctacagcggt
     8641 ttcagcaatc ggcattaaca acatatctgc tttgttaatt gccttccaac cttctacgga
     8701 cgaaccgtcg aacattttgc cttcctcgaa taagtcttcc aaatcgttgg caactaagct
     8761 gacagggagc gaaaccccgt gttctttacc tttaatatca gtaaatttta ataagacaaa
     8821 tttaatatca ttgtcttgaa tcagtttaga tacattagcg attgcgtttg acatagtaaa
     8881 ataagtcctc gttgggataa atgaataata aaaaagctcg gttatcatag cattttttaa
     8941 ccaagtgtgt gatgtttttt agtgtttcaa agctaaaaga agttgcagta atgttgcaaa
     9001 tttttgctaa aatcttaccg cttgcatatt gtttgtatgg tggggagaga aaagcgtgta
     9061 atgttgccga tttatcatat tctaggctcc gagattgagc atcataacca acaggtttta
     9121 accttttttc agcaagagct tttgccgtta ttgccagact ccgaacattt tttttatgtg
     9181 gtaggcaagc cttcactgca aactgctttt actcatttgc ggattaagca gttctcttca
     9241 caaaaagcga ttgcgaacgc cttaattgca aaaaaacggc aaaatccgac cgcttgtttt
     9301 ctgttacacg ggcagtttaa cccttggatt tggctggcga tagccttagg cagattgccg
     9361 accaccaacc tgatttggca tatttggggg gcggatttat atgaaatggc aacttcgtgg
     9421 aaatttaagc tgttttaccc gtttcggcgt gttgcccaac aaaaaatctt acaggtttgg
     9481 gcaacaagag gcgatctgga tcatttgtgg cgaaatatgc gtgcaagatc agaacaggat
     9541 catttaatct atttccccac gaaattaggt gatcaaataa aaattagccc cacacaaaat
     9601 gatcgcttga cgatattgct cggcaattca ggcgatccag cgaatgagct tattcaagcc
     9661 ttaagcgaga ttcatcaacg actagggaac aatgtgcgga ttatcatacc aatgggctac
     9721 ccggaaaata atgaaagcta tattcggcag gtggaagcct atagcaagcg gttatttttg
     9781 gcggaaaatt tgcaaatttt gcggaagaag ctgagctttg aagagtatgt tacgttattg
     9841 agccattgtg atctcggcta tttcaatttt aaacgccagc aaggcattgg cacgataagc
     9901 cttttgatta gccataatgt accttgtgtg ttacattcag ccaatccgtt tgggttggat
     9961 ctacaggcag aaaaagtgcc ttatttgttg gcggaaaatc tgactgcttc acagattcaa
    10021 acaaccaaac aacaactaaa gagcatagat aaaaaccgca ttgcgttttt tgctccgggc
    10081 tacactaaac tatggcaaca gcgattaatc gaattaatgc aaaatgaata atttaatacc
    10141 acttggcgga ttttatcttg tttctgtcgg gctatttttc agcttacttt atcaacttta
    10201 tcagaaatcg gggatttctt tccatttact gtttagtttg ctctattttg ttacttttta
    10261 cgccggtttt ccgttttcgt taataatggc atttggcttt gattacccgc tgccggagct
    10321 aaaaacacaa tggcaagttt ttacggcagc aggtgttggc tatttgattt attacgttgt
    10381 ctattcacgc ttgtggagtg ctgtgtcgca accaactgct cttgttgcaa attttcaggc
    10441 aaaaacgacc gcctgtatcc ttgccgctgt ggcagttgtt gccctgctat atttccttta
    10501 tttaaacggt tttctgcttt tcaaactcga aaaatatagc caactgtttg ccaaagatag
    10561 ggtcatactt tcggcattaa aacgattttt ctacttttta ttgccggcgt tgttaatttt
    10621 ttgcttccta tataagaata aaacagcgtg gtgggcgtta ctgattgttg gtggggcatt
    10681 cggcatatta agctatgtgg cagtgggcgg cacacgggca aatttagcac tagcggttgg
    10741 ctttttctta ttgattggat tgcagcaagg ctatctgtcg ttatcaaagg tgattgccgt
    10801 aagcctaatc ggcgtggtgg cgatgttctt cctcgctcta gcccgttaca aacttaccct
    10861 ttccggtagc gagatgattt acaccttctt atatttaagc cgagatactt tttcgccttg
    10921 ggaaaatttg gctcgtgtat tttctaatga ggtggaatat caaggtttaa tgccgattgt
    10981 gcgggatttc tatgtatata tcccacaatc tttatggcaa gaacgccctg agatcgtctg
    11041 gaatacggcg aattatttta ccaaagtgat tttaggcaac caatcagggt tagctatttc
    11101 gcctacctta ctcggctctt tctatattat gggcggattt agcgttatgg cgttgggaat
    11161 ggggatagta gctgccatga ttaaagcctt agataaccta ttcgcttacg ccaaagcgtg
    11221 caattccgcc ttgttgcaag cctactgctt cggcaattta ttcaatttga tcgtattagt
    11281 gcgagaggga atcgatgcat ttgtttcccg tttttgcttt ttcacgctga tatttatggc
    11341 ttgctatttg ctggcgaaaa tcctttcaat ggggaaaaat aataatgttc gataaagtga
    11401 tcattcgggg cattgcgtta caagcggtca aaaatcggga aatttttgca aattggctga
    11461 tgaatgaaaa gcggattcag ttcggaagat taatggcaat taatgctgag aaagtgattc
    11521 tgtcggaaaa aaatccgttg ttacgccgag tgatagatga agccgagttc aactatgcag
    11581 acggcatcag tgttgttcaa tcgattcgaa aaaaatatcc aaactataaa atagagcgaa
    11641 ttgccggagc cgatttatgg gaaaccttaa tgcaaagagc cggagcgtta aatgtgccgg
    11701 tgtttttggt cggcagctcg gcggataccc taacgaaggt ggaagcaaaa ttggcacaat
    11761 ggaaggtgaa tattgtcggt aggcagaatg gctattttaa ggcggagcaa gaacaggacg
    11821 ttatagagca aattaaagca agtggcgcga agtttattag tgtggcaatg ggaactccaa
    11881 aacaggaact ttttatccaa cagttacaac aacaatatcc acaagcctta tatatgggtg
    11941 ttggcgggac ttacgatgtg tttgccggta aagtgaaacg agcacctaaa atttggcaaa
    12001 atttggggct ggaatggctt tatcgactat tgaaacagcc aacccgctgg caaagacaat
    12061 taaatttact aaaatatgcc tattactatc tgacaaatca gctctaaaaa acgcattttt
    12121 tgatatttat taaacgtttt gaataaaata aaaccaaaca atacattttt ttacattttt
    12181 tttaaaaagt gctagacaag ggaaagtgaa atctataata tacgcctccg ttgcgacgca
    12241 ttaagttgaa ttgatgtaaa tcgaagcgtt cgtagctcag ttggatagag cgttggcctc
    12301 cggagccaaa ggtcgcaagt tcgaatcttg tcgagcgcgc cagatgtcaa tgcggcacta
    12361 tcaacacaat ggtggctata gctcagttgg tagagccctg gattgtgatt ccagttgtcg
    12421 tgggttcgaa tcccattagc caccccattc aatactcttg tgagtatcag acggcgagta
    12481 gcgcagcttg gtagcgcaac tggtttggga ccagtgggtc gtaggttcaa atcctatctc
    12541 gccgaccaat ttattttttg ttctttaaca atttatcaga caatctgtgt gggcacttgt
    12601 tgattgactt gattgaaaaa cattttttta attttgaagt cttaataggt gctaactaga
    12661 aattcattac tttcttttaa ctt
//
LOCUS       DANJWC010000063        12574 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.063, whole genome shotgun sequence.
ACCESSION   DANJWC010000063 DANJWC010000000
VERSION     DANJWC010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12574
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.063"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            126..2402
                     /gene="gshAB"
                     /locus_tag="PWX02_001268"
     CDS             126..2402
                     /gene="gshAB"
                     /locus_tag="PWX02_001268"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HDL1729283.1"
                     /translation="MKLQKLIRENHLALLFQQGNFGLEKESQRVDRNGNIVTTPHPAV
                     FGNRSYHPYIQTDFAESQLELITPPNAKLEDSFRWLSAIHEVVLRSLPEEEYIFPLSM
                     PAGLPPEEQIQEAQFDNPEDVQYRQHLSKNYGKYKQMVSGVHYNFQLSTEFVEKAFAL
                     QTEYSELKAFQNALYMKLANNFLRYQWILLYLLAATPTVESQYFYEKSPLARGQLVRS
                     LRSSPYGYVNSEKVVVNHDSLESYVESLEANVANGLLIAEKEFYSNVRLRGGKKAREL
                     LENGVKYAEFRLFDLNPFAPYGIELNDAKFIHYFLLGMLWLEETSGQKEVEIGKQKLY
                     QVALEDPRTETAFRAEGEAILNQLLAMLEELSAGQESLDLVREKLAQFADPSKTLNGR
                     LIAAIEEVGSYKALGAKLAQTYKAQAFERFYALSAFDNMELSTQALFFDLIQQGIEVE
                     VLDENDQFLALKFGDHLEYVKNGNMTSRDQYISPLIMENKVVTKKVLAKAGFNVPKSV
                     EFTNLEQAVAHYPLFEGKAVVIKPKSTNYGLGITIFQQAVKNREDFAKAVEIAFREDK
                     EVMVEDYLVGTEYRFFVLGDETLAVLLRVPANVVGDGIHSVKELVERKNDDPLRGDGS
                     RSPLKKIALGEIEQLQLKEQGLTVDSVPAKGQTVQLRANSNISTGGDSIDMTDQMHES
                     YRQIAVGIAQAMGAKVCGVDLIIPDLTKPAEPNLSSWGVIEANFNPMMMMHIFPYQGK
                     SRRLTKAVIKMLFPEIGN"
     gene            2724..3701
                     /gene="manX"
                     /locus_tag="PWX02_001269"
     CDS             2724..3701
                     /gene="manX"
                     /locus_tag="PWX02_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDL1729284.1"
                     /translation="MSIAIIIATHGVAAEQLLKTTEMLIGEQEDVAYIDFVPGENAET
                     IMGKYQALVEGPLSHCDQVLFLVDMWGGSPFNAANRFQDGKSGMDVVAGVNIPMLVNT
                     CMARDDVESLEELVEVALEAGSTGIRSLNHQPEVAEVQTATPAQETAAVQQPEIVDDG
                     RRMTIALARIDDRLIHGQVATVWTKESKVNRIIVVNEGVAKDSVRTTMLKSVAPLGVT
                     AHVVDVPKMIRVYNNPEYADDRVMLLFTNPSDVLALIEGGVPLNSINVGGISHKEGKQ
                     MVSHAVAVDQKDIDAFKAIDAKGVELEVRKVSSDSRQYMMDLLKKHNLI"
     gene            3713..4513
                     /locus_tag="PWX02_001270"
     CDS             3713..4513
                     /locus_tag="PWX02_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005716709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDL1729285.1"
                     /translation="MELSALQIVFLFLVACISGMGSILDEWQTHRPLIACTMIGLVLG
                     DVTTGVIVGGSLELLALGWMNIGAAVAPDAALASIVSTILVIVGRQDISTGIAIAIPL
                     AAAGQVLTYVVRAITVGFQHAADKSIEDGNLSRLDWLHRGALLLQAMRIAIPALIVAL
                     TAGTDAVQTMLNAIPEVVTTGLKIAGGFIAVVGYAMVINMMRAGHLMPFFFAGFVIAG
                     FTDFNLVALGVLGTVIAMVYIHMHPKYNKSQQVVVAAAPKNDLDNRLD"
     gene            4527..5363
                     /locus_tag="PWX02_001271"
     CDS             4527..5363
                     /locus_tag="PWX02_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005564636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HDL1729286.1"
                     /translation="MTTEIKKVTKSDLNAVVRRSNLFQGSWNFERMQALGFAYAMVPV
                     IKRLYPDPNDPGRKEAIKRHSEFFNTQPFVAAPILGVTIAMEEERANGKEIDNAAING
                     IKVGLMGPLAGVGDPIFWGTIRPVFAGLGAVLALKGELLGPILFFALFNIVRLAARYY
                     GVVYGYKKGLDVVQDMSGGLLQKLTEGASILGLFIMGALVQKWTSINVPLVLSTIQKQ
                     DGTTEVTTVQSILDSLMPGLLPLLLTFACMWLLRNRVNAIWIILGFFVIGILGASTGA
                     LA"
     gene            5456..5941
                     /locus_tag="PWX02_001272"
     CDS             5456..5941
                     /locus_tag="PWX02_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HDL1729287.1"
                     /translation="MTNTILILFILLGLGFAIYDQVFMHKLKGTTLLEIRLKKQKTID
                     TWLLIGLIVLSISYGVQNQIQPFTLYLLATCIILSVYLAFFRTPRLLLKQHGFFFANV
                     FFNYDKIYQVNIAEGKADEKILVIDLHSGRRLLADVENAEDLQPVVDFFGGYKKESEQ
                     K"
     gene            complement(6028..6819)
                     /gene="tpiA"
                     /locus_tag="PWX02_001273"
     CDS             complement(6028..6819)
                     /gene="tpiA"
                     /locus_tag="PWX02_001273"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDL1729288.1"
                     /translation="MARRPLVMGNWKLNGDKAFTKTLIADLKAELHGVEGCDVAIAPP
                     VMYLAHAEASLAGCGCGCSDKGLIALGSQNVDVNVKGAFTGDISPEMLKEFGAKYIII
                     GHSERRTYHKESDEFIAQKFKVLKDAGLVPVLCIGESEAENEAGKTEEVCARQIDAVI
                     NAHGVEAFNGAVIAYEPIWAIGTGKSATPAQAQAVHKFIRDHIAAKSQAVADQVIIQY
                     GGSVNDANAAELFTQPDIDGALVGGASLKAPAFAVIVKAAAAAKA"
     gene            6979..7998
                     /gene="truD"
                     /locus_tag="PWX02_001274"
     CDS             6979..7998
                     /gene="truD"
                     /locus_tag="PWX02_001274"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HDL1729289.1"
                     /translation="MKLNYLYSPPQAVGRLKAEYADFIVREELGYELAGEGEFVAVKI
                     RKTSANTLFVGEKLAKFVGISEREMSYAGLKDRHAVTEQWFCLHLAGKETPDFSTFEC
                     EGVEVLEVTRHNRKIRVGSLAGNHFELLLRDVTESEELIYRLQQLQAVGFPNYFTEQR
                     FGRDGHNLTQALRWANGEISVKDRKKRSFYLSAARSEVFNLVVSQRISDGLIQKVLKN
                     DYVQLAGSNSFFCVKSDEIAETQARLTQQDLLLTAPLIGERSLELECNPAEKTIIEQH
                     AQLVALMQKERMAAARRAMLCKPQNLEWQFEQEGLRLKFFLDSGSYATALVRELILLA
                     EQNEA"
     gene            8161..8949
                     /gene="surE"
                     /locus_tag="PWX02_001275"
     CDS             8161..8949
                     /gene="surE"
                     /locus_tag="PWX02_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HDL1729290.1"
                     /translation="MRILISNDDGFHAVGIQTLAKTLREAGHSVTIIAPDRNRSAASS
                     CLTLVDPLRVHRFDEYNYAVIAGTPADCVHLALNGLFDGKFAEKFDLVVSGINHGANL
                     GDDVVYSGTVAAALEGRHLPLPSIAVSLVGRKHSSFLDGENHFGTAAQVVLDLLEKME
                     NQPFPQNHVLNVNVPNLPYSELKGTMITRLGARSTAAEIVKQKDPRNADVYWIGVTGD
                     PIDESFGTDFYALNRDCVSITPIQVDMTAHKTIATLKGIFNETV"
     gene            8936..9520
                     /locus_tag="PWX02_001276"
     CDS             8936..9520
                     /locus_tag="PWX02_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDL1729291.1"
                     /translation="MKLFETIYDKTMQWSKHRFAAFWLSFVSFIEAIFFPIPPDVMLI
                     PMSMAKPQNAFKYAVYTTLASVAGGVIGYFIGLYATDWVQGVIANWGMQANFDKAKLW
                     FETWGVAVVFLAGFSPIPYKVFTICAGVMQMAFFPFVITAAISRFARFILVAKLSAWG
                     GKKYEQKIRRSIELIGWGTIVLAVVAYIIYTLMK"
     gene            9538..10038
                     /locus_tag="PWX02_001277"
     CDS             9538..10038
                     /locus_tag="PWX02_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL1729292.1"
                     /translation="MKKSLFVLSLISFALVGCSSNSGESTSAVETTEANATWQSADSI
                     QTAPMPASMNQPIATQPTYQQPTYSAPQPISAAPAPMPVSSGSDSSQVETVGNCQVVR
                     DANNAPIYSQITKGCYTESHYTVGKSDTLYLIGYLTGTNANQIAQLNGLNTGSPLKVG
                     QVLRVR"
     gene            10051..10251
                     /locus_tag="PWX02_001278"
     CDS             10051..10251
                     /locus_tag="PWX02_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729293.1"
                     /translation="MNRTLLAFAISCAFLTACSSPTQPQVEDPNMLPAGTLQPIEGSG
                     ASQDSYSWPSDIQSTSMPDSMK"
     gene            10270..11550
                     /locus_tag="PWX02_001279"
     CDS             10270..11550
                     /locus_tag="PWX02_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL1729294.1"
                     /translation="MKKQFILLPLVSAVLAACSSNNPAPVVNASGNNELSPGVMQPVD
                     VSAGAINSSGGWQSDIQSAPMPSSMNTTPVQTMPQPVSVPQPVATVPQPITNPVITEQ
                     PPQPTTTTKIVKKTKTVEKKVDQNFEIPRDANNAPMYNQIQKGFYDGSTYTVRKGDTM
                     FLIAYIVGKDVKEIAALNNMSEPYQLSVGQKLKTGKSATKTITVEEKVTVPVEPQITY
                     QQGANGTTYASGGNITGPVKAGTANADVTPVNNGIKATTATAPAVTASVGTVNATEDR
                     TASTIRATDGTASGAVQSSNTVSAPASSIKWQWPTSGRVISGFSSAEGGNKGIDIAGN
                     KGQDVRAAAAGKVVYAGNALQGYGNLIIIKHTDDFLSAYAHNNTIEVDEQETVKAGQK
                     IATLGSTGTNTNKLHFEIRYKGKSVDPARYLPRK"
     gene            11790..11981
                     /locus_tag="PWX02_001280"
     CDS             11790..11981
                     /locus_tag="PWX02_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729295.1"
                     /translation="MDPTEPSHRIYILLSEDRFQAEVFSTKLSESSMLLEAVKGGYIS
                     KDGKVRLLKKAEGWKIYYE"
     gene            11974..12540
                     /locus_tag="PWX02_001281"
     CDS             11974..12540
                     /locus_tag="PWX02_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDL1729296.1"
                     /translation="MSETKIRCDWCKSSEIYMKYHDEEWGKPEFDSLKLFEKICLEGQ
                     QAGLSWITVLKKREAYRQAFHQFNPEKIAKMGEEEIDLLMQNTSLIHHRAKLEAIIKN
                     AKAYITMQQNGEDFSRFIWSFVNYQPQINDVPTLEAVSAHTETSKAMPKALKKRGVVF
                     VGETTCYAFMQSMGLVNDHVNNCVFKHK"
BASE COUNT         3719 a         2587 c         2717 g         3551 t
ORIGIN      
        1 gaagcagtat ttctgttata accaaatcat ctacttcatt ccaataaact aaaacccact
       61 atttcactaa gtctttcatt tgtgttattg tgccgataca tttttttgac tacaaaggat
      121 agatgatgaa actacaaaaa cttatccgag aaaatcattt agcattgcta tttcaacaag
      181 ggaattttgg gcttgagaaa gagagccaac gggttgatcg caacggcaat atcgttacta
      241 ctccccaccc tgccgtattc ggtaatcgct cttaccaccc ttatattcaa accgattttg
      301 ccgaaagtca gctagagtta atcactcccc cgaatgctaa attagaagac agttttcgtt
      361 ggctctctgc tattcacgaa gtggtattgc gttcgttgcc agaggaggaa tatatcttcc
      421 ctctcagtat gccggcaggc ttgccgccgg aagagcaaat tcaagaagca caatttgaca
      481 accctgaaga tgtgcaatat cgccaacacc tttccaaaaa ctatgggaaa tataagcaaa
      541 tggtaagcgg cgtgcattat aattttcaac tttcaacgga atttgtcgaa aaagcctttg
      601 ctttacaaac ggaatatagc gagctgaaag ccttccaaaa cgcactttat atgaagttag
      661 ctaataactt tttgcgttat caatggattt tgctctattt attggctgcc acgcctacgg
      721 tggaatcgca atatttttat gaaaaatcac cgcttgcgag agggcaactt gtccgcagct
      781 tgcgttcaag cccttatggt tatgtgaatt cggaaaaagt ggtggttaat cacgacagct
      841 tagaaagcta tgttgaaagc cttgaagcca atgttgccaa cggattatta attgccgaaa
      901 aagaattcta ttccaatgtg cgtttacgag ggggcaaaaa agcccgtgag ctattggaaa
      961 acggtgtgaa atacgccgaa ttccgtttat ttgatttaaa ccctttcgcc ccttacggca
     1021 ttgagctgaa tgatgccaaa tttatccatt atttcttact cggtatgctt tggctggagg
     1081 aaacaagcgg tcaaaaagag gtcgaaatcg gcaaacagaa actttaccaa gtcgcacttg
     1141 aagatccaag aacagaaacc gctttccgtg ccgaaggtga agccatttta aatcaacttt
     1201 tagcgatgct tgaagaatta tctgccggtc aggaaagcct agatttggtg cgggaaaaat
     1261 tagctcaatt tgctgatcca agcaaaaccc tgaacggacg tttaattgcc gcaatagagg
     1321 aagtcggcag ttataaagca ttgggggcta aattggctca aacctataag gcacaagcct
     1381 ttgagcgttt ttatgcgtta tcggcatttg ataatatgga actttccacc caagccctgt
     1441 tttttgattt gatccaacaa gggattgagg tggaagtact ggacgaaaac gatcaattcc
     1501 tcgccctgaa atttggcgat catttggaat atgtgaaaaa cggcaatatg accagccgag
     1561 atcaatacat ttcaccgctg attatggaaa acaaagtggt gacgaaaaaa gtattggcaa
     1621 aagccggctt taacgtgccg aaaagcgttg aatttaccaa ccttgaacag gctgtggcac
     1681 attatccgct atttgaaggc aaagcggtgg tgattaagcc gaaatcgact aactatggtt
     1741 tagggattac catcttccaa caagcggtca aaaatcgaga agattttgca aaagcggttg
     1801 aaattgcgtt ccgtgaagac aaagaggtga tggtggaaga ctatttagtc ggtactgaat
     1861 accgcttctt tgtactgggc gatgaaaccc ttgcggtgtt actgcgtgta ccggcgaatg
     1921 tagtgggaga tggcattcac agtgtaaaag agctggtgga gcgtaaaaat gacgatccgc
     1981 tacgtggcga tggctctcgt agcccgttga aaaaaattgc cttaggtgaa attgagcagt
     2041 tacaacttaa agagcaaggc ttaaccgtag attccgtacc ggcgaaaggt caaacagttc
     2101 aactgcgtgc caattccaac atcagcaccg gcggcgattc gattgatatg accgaccaaa
     2161 tgcacgaaag ctatagacaa attgcagtgg gaattgctca ggcaatgggg gctaaagtgt
     2221 gtggggtgga tttgatcatt cctgatctca ccaaacctgc cgagccaaat ttaagctctt
     2281 ggggcgtcat tgaggcaaac ttcaacccaa tgatgatgat gcacattttc ccttaccaag
     2341 gcaaatcccg ccgtttaacc aaagcggtaa tcaaaatgct gtttcctgag ataggcaatt
     2401 aaattttctc tctatacctc ttttaaggag aatttactca agaattctcc ttaatttatt
     2461 tctaaagttt tactccaatt aaatttcact ttgtgcaaaa cactccaaaa gaagaatttt
     2521 tttgtataag taaaaacgat cgaaaatatc tccgttaaca aaatgttata aagaaaagac
     2581 atttagccca atttatagcc aaaaaagctt attgtctgtg atatagatca cacttttgta
     2641 attaatacat tgtgtgaaat aattatcata ttaaaatctc gccccagtaa ataaacaact
     2701 taaaaaaccg caaggagtaa caaatgagca ttgctattat catagcaacc catggtgttg
     2761 ctgcagagca acttctcaaa acaactgaga tgttgattgg tgaacaagaa gatgttgctt
     2821 acattgactt tgttcccggc gaaaatgcag aaacgattat gggcaaatat caagcattgg
     2881 ttgaaggtcc actatcacac tgtgaccaag tgctgttttt ggtggatatg tggggaggaa
     2941 gcccattcaa cgccgcaaac cgattccaag atggaaaatc gggtatggat gttgttgcgg
     3001 gtgtgaatat tcctatgtta gtgaacacct gtatggctcg tgatgatgtg gagtcgttgg
     3061 aagaattggt ggaagtcgca ttagaagccg gctcaaccgg gattcgttca cttaatcacc
     3121 aacctgaagt ggctgaggtg caaaccgcta ctcctgcaca agaaactgca gctgttcaac
     3181 agcctgaaat tgttgatgac ggacgtcgta tgaccattgc ccttgcccgt attgatgacc
     3241 gcttgattca cggtcaagtt gcgacggttt ggacaaaaga aagtaaagtc aatcgcatta
     3301 ttgtggtaaa cgaaggtgta gcaaaagata gtgtacgaac tactatgcta aaaagtgttg
     3361 ctcctcttgg cgtaacggct cacgttgttg atgtacctaa aatgattcgt gtctataaca
     3421 accctgaata tgccgatgat cgtgtaatgt tactctttac caacccaagt gatgtcttag
     3481 ccttaattga aggcggtgta ccactcaata gcattaacgt gggcggtatt tcgcataaag
     3541 aagggaaaca aatggttagc catgctgtgg cggttgatca aaaagatatt gatgccttca
     3601 aagcaattga tgcgaaaggt gttgaattag aagtacgcaa agtctcaagc gatagccgtc
     3661 agtatatgat ggatttattg aaaaaacata acttaatcta aaggaaatta ctatggaact
     3721 ttcagcatta caaattgtgt ttttattcct cgttgcctgt atctcaggta tgggctcaat
     3781 tcttgatgaa tggcaaaccc accgtccgtt aattgcctgt acgatgatag gcttagtgct
     3841 tggtgatgtg actaccggcg ttattgttgg cggctcatta gaattattag ccttaggttg
     3901 gatgaacatt ggtgccgctg ttgcaccaga tgctgcatta gcctctattg tctcaaccat
     3961 tttagtcatt gtaggtcgtc aagatatttc aaccggtatc gccatcgcta ttccgcttgc
     4021 agctgccggc caagtattaa cctatgttgt gcgtgcaatc accgtgggtt tccaacacgc
     4081 agcagataaa tcaattgaag acggtaactt aagccgttta gactggttac atcgcggtgc
     4141 tttattgctt caagcaatgc gtattgccat tccggcatta atcgtggcat taacagcagg
     4201 tacagatgcg gttcaaacaa tgttaaatgc cattcctgaa gtggttacaa ccgggctgaa
     4261 aattgccggt ggcttcattg ccgtggtagg ttatgcgatg gtaatcaata tgatgcgtgc
     4321 aggtcattta atgccgttct tcttcgctgg ctttgtaatt gcaggcttta ccgattttaa
     4381 ccttgttgcc cttggagtat taggtacggt tattgcaatg gtttatatcc atatgcaccc
     4441 taaatataac aaaagccagc aggtagtggt ggcagctgca ccaaaaaatg atttagacaa
     4501 ccgtttagac taagagggca tttaaaatga caactgaaat caaaaaagta acaaaaagcg
     4561 atttgaatgc cgttgtacgt cgttcaaact tattccaagg ttcgtggaac tttgagcgta
     4621 tgcaggctct cggttttgcg tatgcgatgg taccggttat caaacgttta tatccggatc
     4681 caaatgaccc gggtcgcaaa gaagcgatta aacgccattc tgaattcttt aacactcaac
     4741 cattcgtagc tgcaccgatt ttaggggtaa cgattgcgat ggaagaggaa cgagccaacg
     4801 gtaaagaaat cgacaatgcc gcaattaacg gtattaaagt tggtttaatg gggccgttag
     4861 ccggtgtggg tgatccaatt ttctggggaa ccattcgccc tgtatttgcc gggttagggg
     4921 cagtcttagc cttaaaaggc gagcttttag gtccaatcct gtttttcgcc ttatttaaca
     4981 tagtgcgttt agcagcacgc tattatggcg tagtgtatgg ctataaaaaa gggttagatg
     5041 ttgtgcaaga tatgagcggc ggtttattgc aaaaattaac cgaaggtgct tcaattttag
     5101 gtctgtttat tatgggggca ttggtacaaa aatggacgag cattaacgtt ccgcttgtgc
     5161 tttcaaccat tcaaaaacaa gacggtacaa ctgaagtaac aacggttcaa agcattctcg
     5221 atagcttaat gccaggctta ctcccattat tattaacctt tgcttgtatg tggttactgc
     5281 gtaatcgagt aaatgctatc tggattatcc tcggtttctt tgttatcggt atcctcggcg
     5341 cttcaaccgg tgcattagct tagttgcaaa attttctgtg aaaaagaccg cttgaacttg
     5401 aacattcaag cggtttttta tacgctttag cctaaataaa ttcacttgct taattatgac
     5461 caataccatc cttattctct ttattttact tggcttaggg ttcgccattt atgaccaagt
     5521 ttttatgcac aaactcaaag gaacaacctt actggaaatc cgtttaaaaa agcaaaaaac
     5581 aattgatact tggttattga ttggtttaat cgtcttgtca attagctatg gcgtacaaaa
     5641 tcaaatccag ccctttacac tctacctgct ggcgacgtgc attattctgt cggtctatct
     5701 cgcctttttc cgcacccctc gcttgttgtt aaaacagcac ggttttttct ttgcgaatgt
     5761 cttttttaat tacgacaaga tttatcaagt caatattgca gaaggaaaag cagacgaaaa
     5821 aattttggta attgatttac atagcggaag aaggctactt gccgatgttg aaaatgcgga
     5881 agatcttcaa ccggtagtgg atttttttgg cggttataaa aaggaaagtg agcagaaata
     5941 gtttgcaaac aaaacaacaa gcggtcattt ttccctgaaa ttttgcaaaa aaccgaggaa
     6001 agatgaccgc ttgtaaaatc ggttagatta ggctttagca gccgctgccg ctttaacaat
     6061 caccgcaaat gcaggcgctt taagtgaagc accacctact aatgcaccat caatatccgg
     6121 ttgagtgaat aactctgctg cattggcatc gtttacagag ccaccgtatt gaatgatcac
     6181 ttggtcagcc actgcttgtg atttagctgc aatatggtca cgaataaatt tatgtactgc
     6241 ttgtgcttgt gccggagtgg ctgatttgcc ggtaccgatt gcccaaattg gttcgtaagc
     6301 aataactgca ccgttgaatg cttccacacc gtgtgcgttg atgaccgcat caatttgtct
     6361 tgcacacact tcttcggttt tacctgcttc attttcggct tcagattcac cgatacataa
     6421 tacaggcact aagccggcat cttttaatac tttgaatttt tgagcaataa attcatcgct
     6481 ttctttgtgg taagtacggc gttctgaatg cccgataata atatatttcg ccccaaactc
     6541 ttttaacatt tcaggtgaaa tatcaccggt gaatgcccct tttacgttca catccacgtt
     6601 ttgtgaacca agtgcgatta agcctttgtc gctacaacca cagccacaac cggcaagtga
     6661 tgcttcagcg tgtgccaaat acatcaccgg tggagcgatt gctacatcac agccttcaac
     6721 gccgtgtaat tctgctttta aatctgcaat cagggttttg gtaaaggctt tatcaccgtt
     6781 taatttccag ttacccatta caagtggacg acgtgccatt ttgtttctcc tatatgaagt
     6841 tgaacttaaa tctaacatca agcactatac caaattttgt agccgttttg tgctttttat
     6901 tttgcttttt atgatctaga tcgaaaggtt taacgtataa tttctcaatc ttacgtatat
     6961 ccttaatatg agaacctgat gaaactgaat tatttatact ctcctcctca agctgttggg
     7021 cgactaaaag ccgaatatgc agactttatt gtgcgtgaag aactgggcta cgaactagcc
     7081 ggcgaagggg aatttgtggc ggttaaaatc cgcaaaacca gcgccaacac gctgtttgtg
     7141 ggggaaaagt tagccaaatt tgtcggcatt tccgagcgtg aaatgagtta tgcgggctta
     7201 aaagaccgcc acgctgtgac tgagcagtgg ttctgcttgc atttagcagg caaagaaacg
     7261 cccgatttct ccacctttga atgcgaaggc gtggaagtgc tggaagtgac ccgccacaac
     7321 cgcaaaattc gggttggctc tttagccggc aatcattttg aattattact gcgtgatgtc
     7381 acagaaagcg aagagttgat ttaccgtttg caacagttac aagcggtcgg atttcccaat
     7441 tattttaccg aacaacgctt tgggagagac ggacataacc tgactcaagc cctgcgttgg
     7501 gcaaatgggg aaattagcgt gaaagatcgc aaaaaacgta gtttctacct ttctgctgcc
     7561 cgctcggaag tgtttaattt agtggtttca cagcggatta gtgacggctt aatccaaaaa
     7621 gtattaaaaa atgattacgt tcaacttgcc ggctctaaca gctttttctg cgtgaaatcg
     7681 gacgaaattg ccgagactca agcccgttta acacagcaag atctacttct caccgccccg
     7741 ttgataggcg aaagatcgct tgaattagaa tgcaatcctg ctgaaaaaac tatcatcgaa
     7801 caacacgctc aactggttgc attaatgcaa aaagagcgta tggcagccgc tcgccgagct
     7861 atgctttgta agccacaaaa tcttgaatgg caatttgaac aagagggttt acgtttaaaa
     7921 ttctttttag attcaggcag ttatgccact gcactggtgc gagagttaat tctacttgcc
     7981 gaacagaacg aagcctaagc actcacaagc ggtaagattt aaatgatttt ttgcaatttg
     8041 taaaactttg gtaaaatctt accgcttttt acactttaga ctaaaaaaac tgtcgaaaaa
     8101 ttgttagaat tgcgatcttt gtatttagac tctatcaaga tgtaagatct aaaggaaatt
     8161 atgcgaattt taattagtaa tgacgatggt tttcacgctg tcggcattca aaccttagcc
     8221 aaaaccttgc gtgaagccgg acatagcgtg accattatcg caccggatcg taaccgcagt
     8281 gcagcttcaa gttgcttaac cttagttgat ccgctacgag tgcatcgttt tgatgaatat
     8341 aattatgcag tgatcgccgg cacgcctgcc gattgcgtac atttagcctt aaacggctta
     8401 tttgacggca aatttgcgga aaaatttgat ttagtggtct ccggcattaa tcacggagcc
     8461 aatttaggcg atgatgtagt ttactccggc acggtagcgg cagcgttaga aggtcgccac
     8521 ttaccgttac cgagtattgc agtatcactg gttgggcgta aacattcttc gtttttagac
     8581 ggtgaaaatc attttggaac ggcagcacag gtagtgttag atttattgga aaaaatggaa
     8641 aaccagccat tcccacaaaa ccacgtccta aacgtgaatg tgccgaattt accttattca
     8701 gagctaaaag gcacaatgat cacccgcttg ggtgctcgtt caactgcagc ggaaattgtg
     8761 aagcaaaaag atcctcgcaa tgccgatgtt tactggattg gcgtcaccgg tgatccgatt
     8821 gatgaaagtt ttggcaccga tttttatgca ttaaaccgag actgtgtctc tattaccccg
     8881 attcaggtgg atatgaccgc tcataaaacg atagcaacct taaaaggcat ttttaatgaa
     8941 actgtttgaa acaatttatg acaaaacaat gcagtggtct aagcaccgtt tcgctgcttt
     9001 ttggttgagt tttgtcagtt ttattgaagc gatctttttc ccgattccgc cggatgtgat
     9061 gttgattccg atgtcgatgg caaaaccgca aaatgcgttt aaatatgcag tttacactac
     9121 ccttgcttcg gttgccggtg gtgttatcgg ctattttatc ggcctatatg ctaccgattg
     9181 ggtgcaaggc gtcatcgcaa attggggaat gcaagcaaac tttgataaag cgaaattatg
     9241 gtttgaaact tggggggttg cggtagtatt tttagccggt ttttcgccga ttccctataa
     9301 ggtatttacc atctgtgccg gtgtaatgca gatggcgttc ttcccgtttg tgattactgc
     9361 tgcgatttct cgctttgccc gttttatttt ggtagcaaaa ctctctgctt ggggtggcaa
     9421 aaaatatgaa caaaaaattc gccgctcaat tgaattaatt ggttggggaa caatcgtttt
     9481 agccgttgtt gcctatataa tttatacatt aatgaaataa attttaagga tataaaaatg
     9541 aaaaaatcac tttttgtttt atctctgatt tcttttgctt tagtgggttg ttcaagcaat
     9601 tcgggtgaat ccacttctgc ggtagaaaca acagaagcaa atgcaacttg gcaatcagca
     9661 gatagcattc aaactgcacc aatgcctgcc tcgatgaatc aacctattgc tactcagcca
     9721 acctatcagc agccgactta ctctgcacca caaccaattt cggcagcacc agcaccaatg
     9781 cctgtttcaa gtggtagtga tagctctcaa gttgaaacag tagggaactg ccaagtggtg
     9841 cgtgatgcga ataatgctcc tatctattca caaattacca aaggctgcta cacagaaagt
     9901 cactatacgg taggtaaaag tgatacgctt tatttaatcg gttatctaac cggcaccaat
     9961 gccaatcaaa ttgcgcagct aaatggttta aataccggct ctccactgaa agtcgggcaa
    10021 gtcttacgtg ttcgttaata ggcagagaaa atgaacagaa cccttttagc atttgcaatt
    10081 tcttgtgcat ttttgaccgc ttgtagctcg cctactcagc cacaagttga agatccaaat
    10141 atgttacctg caggcacatt acaaccgatt gaaggctcag gcgcaagcca agacagttac
    10201 agttggccgt ctgatattca atcaacgtca atgcctgaca gtatgaaata attttaggac
    10261 aatcatttaa tgaaaaaaca atttattctt ctccctttag tctctgctgt tttggcagcc
    10321 tgttcttcaa acaatccagc accggtggtg aatgcttccg gtaataacga attaagcccg
    10381 ggcgtgatgc agcctgttga tgtgagtgca ggtgcaatta attcaagcgg tggctggcag
    10441 tctgatattc aatctgcacc aatgccaagc tcgatgaata ccactccggt gcaaacaatg
    10501 ccccagccgg tttcagtgcc acaacctgtg gcgactgtac cgcagccaat taccaatccg
    10561 gtgattaccg aacaaccacc acagccgaca accacgacca aaatcgtgaa aaaaacgaaa
    10621 acggtcgaga aaaaagtgga tcaaaatttt gagatcccgc gtgatgccaa caatgcccca
    10681 atgtacaacc aaattcaaaa agggttctac gatggctcta cctataccgt acgcaaaggc
    10741 gacacaatgt ttttgattgc ctatattgtg ggtaaagatg taaaagaaat cgccgctctt
    10801 aataatatga gcgaacctta ccaattaagc gtagggcaaa aactcaaaac cggcaaatct
    10861 gcaacgaaaa ctattaccgt ggaagaaaaa gtcaccgtac cggttgaacc acaaattacc
    10921 taccaacaag gtgcaaacgg cacaacctat gcttctggcg gtaatattac tgggccggta
    10981 aaagcaggca cagccaacgc tgatgtcacg ccggttaata acggtattaa agcaacaact
    11041 gcaactgctc cggcagtaac tgcttctgtc ggtacagtaa atgcaacaga ggaccgtacc
    11101 gcctccacca ttcgtgccac agatggcaca gcaagtggtg ccgtacaaag tagtaatact
    11161 gtttctgcac cagcttcatc aattaaatgg caatggccaa ccagcgggcg tgtaatttca
    11221 ggcttctcct cagcagaagg aggtaataaa ggcattgata ttgccggcaa taaaggacaa
    11281 gatgtacgtg ctgccgccgc aggtaaagtg gtatatgccg gtaatgcctt gcagggttac
    11341 ggtaacttaa tcatcatcaa acataccgat gatttcttaa gtgcttatgc acacaacaat
    11401 actattgaag ttgatgagca agaaacggtg aaagccggtc agaaaatcgc gactttaggt
    11461 agtacaggaa ccaatacgaa taaattacat tttgaaattc gttacaaagg aaaatctgtc
    11521 gatccggctc gttatttacc aagaaaataa gagtaacttt ttagcataat ccgaccgctt
    11581 gtgtactaca agcggtcaat ttttaggaaa attatgcaaa tgaaaaaata tatcgcacac
    11641 atgataagtt cgttactgct gatagcttgt tcggactacc gagcaacacc aactagccca
    11701 agcgatgctt acacttgctc tccttctttt cgctactctt tcttaaaaaa acgtgcatac
    11761 ctttattcag tatagccgac attcaattat tggatccaac cgagccttca catcgcattt
    11821 atattttgtt atctgaagat cgatttcaag ccgaagtctt ctcaacgaag ctctctgaat
    11881 cttctatgtt attggaggcg gttaaaggag gctatatttc aaaagacgga aaagttcgtt
    11941 tactcaaaaa agctgaaggt tggaaaattt actatgagtg agactaaaat acgttgtgat
    12001 tggtgtaaaa gcagtgaaat ttatatgaaa tatcacgacg aagaatgggg aaaaccggag
    12061 tttgacagcc tcaaactgtt tgagaaaatc tgccttgaag ggcaacaagc cggactttct
    12121 tggattacgg tgttgaaaaa acgggaagcc taccgccaag cattccatca gtttaatcct
    12181 gaaaaaattg ccaaaatggg tgaagaagaa attgatttgt taatgcaaaa cactagctta
    12241 atccaccacc gagcaaagtt agaagccatt ataaaaaatg cgaaagctta tatcacaatg
    12301 cagcaaaatg gcgaagactt cagtcggttt atttggtctt ttgtgaatta tcagccgcaa
    12361 attaatgatg taccaacact tgaagctgtt tctgctcata cagaaacctc aaaagcaatg
    12421 ccaaaagcgt tgaaaaaacg tggggttgtg tttgtaggcg agactacttg ctatgctttt
    12481 atgcagtcaa tggggctggt taatgaccat gtgaataatt gcgtgttcaa acataaataa
    12541 acacaaaaaa ttaggcgacc atttggtcgc ctaa
//
LOCUS       DANJWC010000064        12343 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.064, whole genome shotgun sequence.
ACCESSION   DANJWC010000064 DANJWC010000000
VERSION     DANJWC010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12343
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.064"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            115..780
                     /locus_tag="PWX02_001282"
     CDS             115..780
                     /locus_tag="PWX02_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HDL1729297.1"
                     /translation="MNFTTYFSDENKRRSLILLSLFHIFIIAISNYLVQISFEVQVPL
                     TEFSFPTTWGTLTFPFIFLATDLTVRVFGADLARKIIFVVMFPALVISYVISVLFFET
                     QFQGFSALTEFNFFVFRIALASFAAYLVGQLLDVLVFNSLRQGKRWWLAPTCSTIFGT
                     LIDTFVFFAVAFYKSSDEYMAEHWFTIGSVDYAFKLFVSLLLFLPLYGVILNFIMKKL
                     DLK"
     gene            866..1669
                     /gene="map"
                     /locus_tag="PWX02_001283"
     CDS             866..1669
                     /gene="map"
                     /locus_tag="PWX02_001283"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDL1729298.1"
                     /translation="MNNIPLRNEEEIQKLRVACKLASDVLVMIAPYVKEGVTTGELDK
                     ICHEYILANGATSASLGYHGFPKSVCISLNEVVCHGIPSDDKKLKKGDILNIDVTVIK
                     DGYYGDNSMMYVVGEPSVRDKRLMAVTLESLYVGIRTVKAGVRLKEIGTAIQKYVEKE
                     GFSVVREYCGHGIGTEFHCDPQVVHYATDDGGVVLKEGMVFTIEPMVNAGKKEVRLMG
                     DGWTVKTKDRSHSAQYEHQIVVTKDGCEVLTIRDEEIAEGRISRIMHNS"
     gene            complement(1785..3203)
                     /locus_tag="PWX02_001284"
     CDS             complement(1785..3203)
                     /locus_tag="PWX02_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HDL1729299.1"
                     /translation="MNYPKIAEQTIAKLGGKENITTLAHCATRLRLTIADESKIDKEA
                     IENIEGVKGQFSTSGQYQIIFGSGTVNEVYAQMQKQMGMADMSTSEVAAAGAANQPLL
                     QRLVKGLADIFVPIIPAIVAGGLLMGIHSMLTAVGFFWEGESVVSRYPNISDLVDFIN
                     TIANAPFVFLPVLLGFSATRKFGGNPFLGAALGMLLVHPALADGWNYAKTLMEGNIKY
                     WNIFGLEIEKVGYQGTVIPTIVSAWVLATLEKTFRKFVPSYLDNLVTPMMSLFIAGIL
                     AFTVIGPLGREAGSAISAGLTWLYDSLGFIGGAIFGTFYAPIVITGMHQTFIAVETQL
                     LAEVAQTGGTFIFPIAAMSNIAQGAACLGVAFAMKDAKVRGLAVPSGISALLGITEPA
                     MFGVNLRYRQAFIAAMIGSGLASAFIAFFNVKAIALGAAGFLGIPSIKPDSLAMYSVG
                     MVISFVVAFTISAIWVKKAQAK"
     gene            complement(3510..4160)
                     /gene="thiQ"
                     /locus_tag="PWX02_001285"
     CDS             complement(3510..4160)
                     /gene="thiQ"
                     /locus_tag="PWX02_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein"
                     /protein_id="HDL1729300.1"
                     /translation="MIELDLHFTYEQMNMHFQLSVKKGERIAVVGESGAGKSTLLNLI
                     AGFEKAESQQSQLWLNQKNHLDTEVAQRPVSMLFQDNNIFPHLTVEQNIGLALVPSLS
                     LNPKQKAQVKQIATQMGIETFLQRQADQLSGGQKQRVALARTLLRDKPILLLDEPFSA
                     LDPQRRAELQQIVFDICQSRNLTLLMVTHQLSESLFLFNRVITVADGEIVSDLSPL"
     gene            complement(4157..5731)
                     /locus_tag="PWX02_001286"
     CDS             complement(4157..5731)
                     /locus_tag="PWX02_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HDL1729301.1"
                     /translation="MLSTLTKTTAWFIYFSIIALYVFSLWALMDYHQNDTLFVFSDIT
                     ATVAYSLLQASLSAVISLFLGSLLARSFFYLDFKGKNLLYKTISFIWALPSLVIIFAV
                     IGVFGNSGWLAKLFQQLGWNWQFNLYGLHGIILAHCLFNIPFVMKYYTGGLQLTPSSR
                     FQLAAQLNLTGWNYIQIVELPIIKTLLPYMFMTVFLVCFTSFPIVLMLGGSPKYSTLE
                     VAIYQAVTFEFDFAKALILIGVQFVVGIGLQLVMNYISTSALKKLKQQPNRVEIWKPK
                     PTGLAKLGLQAVIFLQSFAIILPLVSVIWAGLSVSKLSERLLNPTLWQATQYSLYLSL
                     IASIFVITISYLIALEARQLLYAKAKLRYSILTAITLFPLLLPIFLLSVGLFILLMNS
                     ELSSEALLLLVGICNGLTLLPFIYPMLFSAMWNSLTSHDKLAQGLGLKGLRRWWIVEK
                     NQLIRPLVTVFALAMSSSLGSFTVIAFFGNADFSSLPYLLYQQLGSYRTEDAAVTALI
                     LMLFALLPFLFSKDKL"
     gene            5992..6249
                     /gene="ptsH"
                     /locus_tag="PWX02_001287"
     CDS             5992..6249
                     /gene="ptsH"
                     /locus_tag="PWX02_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HDL1729302.1"
                     /translation="MYSKDVVITAPNGLHTRPAAEFVKAAKGFASDITVTSGGKSASA
                     KSLFKLQTLGLTQGTTITISADGEDEQKAVDFLVDLIPTLE"
     gene            6371..8092
                     /gene="ptsI"
                     /locus_tag="PWX02_001288"
     CDS             6371..8092
                     /gene="ptsI"
                     /locus_tag="PWX02_001288"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HDL1729303.1"
                     /translation="MITGIAASPGAVFGKALVLKEEPIVLNTRKITESEVEAEKAKFF
                     TGREKAAAQLTAIKEKARRTLGEEKEAIFEGHLMILEDEELEEEILSYLTDNLVTADV
                     AASKVIEMQASMLAEIDDEYLKERAGDIRDIGNRLVRNILNMNIVDLGDIQDEVILVA
                     YDLTPSETAQLNLDKVLGFITDIGGRTSHTSIMARSLELPAIVGTNNVTSLVKTGDFL
                     ILDAVNNEVHLNPSEEKIANFKALQEKLNAEKEELAKLKELPAQTLDGHKVDVAGNIG
                     TIRDVEGVLRNGGEAVGLYRTEFLFMDRTQLPGEEEQFQAYKEIVEAMNGQMVILRTM
                     DIGGDKELPYLDLPKEMNPFLGWRAVRIGFTRREILDTQLRAVLRASAFGNLAVMFPM
                     IISVEEIRLLKSIVAELKDQLRAEGHAFDENLKLGIMVETPSAAVNARHLAKEADFFS
                     IGTNDLTQYTLAVDRGNEIIAHLYNPLSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     VRATALLLGMGLDEFSMSGISVPHVKKLIRSINFADAKALADEALAQPTAADIERLVD
                     EFYQKLN"
     gene            8177..8677
                     /gene="crr"
                     /locus_tag="PWX02_001289"
     CDS             8177..8677
                     /gene="crr"
                     /locus_tag="PWX02_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005702996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL1729304.1"
                     /translation="MGLFDKLFGSKQKESQEIKIYAPLTGEIVNIEDVPDVVFSEKIV
                     GDGVAIRPVGDTIVSPVNGTIGKIFETNHAFSIESEEGVELFVHFGIDTVELKGEGFT
                     RLAQEGQTVKVGDPIIKFDLALLEGKAKSVLTPIVISNMDEISNLSKLNGQVVAGESV
                     VLTLTK"
     gene            8894..10321
                     /gene="miaB"
                     /locus_tag="PWX02_001290"
     CDS             8894..10321
                     /gene="miaB"
                     /locus_tag="PWX02_001290"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDL1729305.1"
                     /translation="MAKLHITTWGCQMNEYDSSKMADLLNSTHGFELTENPEDADVLL
                     LNTCSIREKAQEKVFSQLGRWKNWKKDKPNLIIGVGGCVASQEGEHIRDRAPYVDIVF
                     GPQTLHRLPEMINQIRGGKSSIVDISFPEIEKFDRLPEPKAEGPTAFVSIMEGCNKYC
                     SFCVVPYTRGEEVSRPVDDVLFEIAQLAAQGVREVNLLGQNVNAYRGETFDGGICTFA
                     ELLRLVAAIDGIDRLRYTTSHPIEFTDDIIEVYRDTPELVSFLHLPIQSGSDRVLTMM
                     KRNHTALEYKAIIRKLRAVRPEIQISSDFIVGFPGETAEDFEQTMKVIEQVNFDMSFS
                     FIYSARPGTPAADLPDNVSDDEKKERLSRLQQRINHQAMQFSRAMLGTEQRILVEGPS
                     KKDLMELTGRTETNRVVNFVGTPDMIGKFVDVKITDVYTNSLRGEVIRTEDQMGLRVV
                     ESAESVIARTRKEDDLGVGKYVVNL"
     gene            10438..11361
                     /locus_tag="PWX02_001291"
     CDS             10438..11361
                     /locus_tag="PWX02_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HDL1729306.1"
                     /translation="MSKIWVTGDAVVDLIPDGEHHYLKCAGGAPANVAVGVSRLGVEA
                     GFIGRVGNDPLGKFMREVLQAENVCTKQMILDAQHRTSTVIVGLDNGERSFTFMVNPS
                     ADQFLEIGDLPEFNQGDFLHCCSIALINDPSRSTTIEGIRRVKKAGGYVSFDPNLRES
                     LWKSLDEMKTVVNSVVAMADILKFSEEELTLLTETETLEQATQAITAQYPEKLIIITL
                     GKDGAIYHFNGKSQVVAGKALKPVDTTGAGDAFVSGLLAGLAQTPNWHDETVLVEVIR
                     KANASGALATTAKGAMSALPNKAELDAFLAE"
     gene            11466..>12343
                     /locus_tag="PWX02_001292"
     CDS             11466..>12343
                     /locus_tag="PWX02_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HDL1729307.1"
                     /translation="MHIFNNGKYKSLHAQSEGELNALRQTVLSDPDFYPTYHLAPETG
                     LFNDPNGLIFDGEKYHIFAQWFPYGALHGMKHWQHFITTDFQTFSKGDLLIPDELFES
                     HGCYSGGAILWQDKIVAFYTGNTRRPSDNARIPHQNIAIFDKSGKLLEKRCIIDQAPA
                     GYTEHVRDPKPYLTAEGKIRFVLGAQRENLTGTALIYEMDDLNATPRLIAELEVHNFD
                     NSNVFMWECPDLFSLGGKDLFVWSPQGKLRESHQFQNNYHATYALGELNGNRLRAEHI
                     EELDYGFDFYAPQTVE"
BASE COUNT         3745 a         2300 c         2713 g         3585 t
ORIGIN      
        1 aattgtttca ttttttgaaa taatatgagc ttgattttta tgggaaaatg tatagaataa
       61 tgcccccttg agttccctta cctcaagttt tcatttaacg aaaaaggata caatatgaac
      121 tttactactt atttttcgga tgagaacaaa cgtcgttctc taattttact ttcacttttc
      181 catattttta tcatcgcaat tagcaactat ttagtgcaaa ttagctttga agttcaagta
      241 ccactaacag aatttagctt tccgacaact tggggaacgt taacattccc atttattttc
      301 ttagcgaccg atttaaccgt gcgtgtattt ggtgcagatc ttgctagaaa aattattttt
      361 gttgtgatgt ttccggcact tgtcataagc tatgtgattt ccgttttatt ctttgaaacc
      421 caatttcaag gctttagtgc attaactgag ttcaactttt ttgttttccg tattgcactg
      481 gctagctttg cagcttattt agttgggcaa ttattagacg ttctagtatt taatagttta
      541 agacaaggta agcgttggtg gcttgctcca acctgttcaa ccatttttgg tactttaatc
      601 gatacttttg tattctttgc tgtcgccttt tataaaagta gcgatgaata tatggcagag
      661 cattggttta ctattggttc ggtcgattat gcgtttaaac tctttgtgag cctactgcta
      721 tttctaccgc tttatggtgt gatattaaat ttcattatga agaaacttga tttaaagtag
      781 aaatctaaac tgaggtttgg ggattgtgct gaaaaaaggt ataatcccct tactttttat
      841 ggcagatata gcagagagca atgtaatgaa taatattcca ttaagaaacg aagaagaaat
      901 tcaaaaacta cgagtagcct gtaaactggc ttcagatgtt ttagtaatga tcgcccctta
      961 tgtgaaagag ggcgtaacaa ctggtgaatt agataaaatt tgtcacgaat acattttggc
     1021 aaatggggca acttcagcca gtttgggcta tcacggcttc ccaaaatcgg tgtgcatttc
     1081 gttaaatgag gtcgtttgtc acggtattcc aagtgatgac aaaaagctga aaaaaggcga
     1141 tattttaaac atcgatgtga cggttatcaa agatggctac tatggcgata actcgatgat
     1201 gtatgtagtc ggtgagccaa gtgtgcggga taaacgctta atggcggtta cgctagagtc
     1261 cctttatgtc gggattcgca ccgtgaaagc gggggtgcgt ttaaaagaaa ttggtacagc
     1321 gattcaaaaa tatgtggaaa aagaaggctt ttcggtagta cgagaatatt gcggacacgg
     1381 tattggtact gaattccatt gcgacccaca agtagtgcat tatgctaccg atgatggcgg
     1441 tgtagtgtta aaagaaggaa tggtgtttac gattgagcca atggtgaatg ccggcaaaaa
     1501 agaagtccgc ttaatgggtg acggttggac agtgaaaacc aaagaccgca gccactccgc
     1561 acaatatgag catcaaattg tagttaccaa agacggctgt gaagtgctga caattcgtga
     1621 tgaagagatt gccgaagggc gtatttctcg aattatgcac aacagctaat ttgcaaattt
     1681 ttagcgaaaa atgaccgctt gcgaaactca ttgttaagct aaggcgttta aaaacaaaag
     1741 ggattaagct aacacttaat cccttttttg tgatctattt ttgattattt cgcttgtgct
     1801 tttttcaccc aaatggctga aatggtaaat gcaactacaa atgaaattac cataccgaca
     1861 gaatacattg caaggctgtc aggtttaatc gacggaatac ctaagaaacc ggctgcacct
     1921 aaggcaattg ctttcacatt aaagaaagcg ataaatgcac tggctaaacc tgaaccgatc
     1981 atcgccgcaa taaaggcttg gcggtagcgt aaatttactc cgaacattgc cggttcggtg
     2041 atacctaaca gggctgaaat acctgacggc acggctaaac cacgcacttt ggcatctttc
     2101 attgcaaaag ctacacctaa acaggctgca ccttgtgcaa tgttagacat tgctgcaatc
     2161 gggaagataa atgtaccgcc ggtttgtgct acttcagcta ataattgggt ttcaaccgca
     2221 ataaaggttt ggtgcatacc ggtaatcacg attggagcgt agaatgtacc gaaaattgct
     2281 ccaccgataa agcctaagct gtcatataac cacgttaagc ctgctgaaat cgctgaacct
     2341 gcttcacgac ccaatggtcc gattacagtg aaagctaaaa taccggcgat aaataatgac
     2401 atcattggcg tcaccaagtt atctaaatag cttggcacaa atttacggaa agttttctca
     2461 agggttgcta acacccaagc cgaaacgata gttggaataa ccgtgccttg ataacccact
     2521 ttttcaattt ctaagccgaa aatattccag tatttgatgt taccttccat taaggttttg
     2581 gcatagttcc aaccgtctgc aagtgccggg tgaaccaata acataccaag tgccgcacct
     2641 aagaatggat taccgccaaa tttgcgtgta gctgagaaac cgagtaatac aggtaagaat
     2701 acaaatggtg cgttagcaat agtgttgatg aaatcaacta aatcggaaat attcggataa
     2761 cggctaacaa cagattcacc ttcccagaag aaaccgactg ccgttagcat tgagtgaata
     2821 cccattaaca aaccgccagc cacaatagcc ggaatgatcg gcacgaaaat atcggctaag
     2881 ccttttacca aacgttgtag taatggctga tttgctgcac cggcagccgc tacttcagag
     2941 gtggacatat ctgccatacc catttgtttt tgcatttggg catacacttc atttaccgtg
     3001 cctgatccaa aaataatttg gtattgaccg gaagtagaga attgaccttt cacgccttca
     3061 atattttcaa tcgcctcttt atcaattttg gattcatcag caatggttaa gcgtaaacgg
     3121 gttgcacagt gggcaagtgt ggtaatgttt tctttgccac ctaattttgc aatcgtttgt
     3181 tctgcaattt ttggatagtt catttgaatt tacctttcat aaaaaataat cggttgcatt
     3241 ttaactaaaa cggtttagct aaaaaagaga agatttaaaa tatttccaaa tttgtgaagt
     3301 agatcacatt ttggggaaga gaatagcgga atagggaggt aatattagtt acagttcccc
     3361 tctttgctaa agtagggggc gagttctgaa aaaaatgaat aacgctagta tccgtgcgga
     3421 acatcattca ccgttagatt gttggaattg acatcgttgc gtacgccagc gtggcgtccc
     3481 tacatttaga acctagcaga tctgctttgc taaagagggg agagatcaga aacaatctcc
     3541 ccatctgcta ccgttatcac ccgattaaat agaaataggc tttcggatag ttggtgagtt
     3601 accattaaca gggttagatt tctgctttgg caaatgtcaa acacgatttg ctgtaattcg
     3661 gctcggcgtt gcgggtcgag ggcggagaag ggttcgtcca ataataaaat cggcttatcc
     3721 cgcaacaaag tacgagctaa tgccactcgc tgtttttgtc cgcccgatag ctgatcggct
     3781 tgacgctgaa ggaaggtttc aatccccatt tgtgtggcaa tttgtttaac ctgtgctttt
     3841 tgtttcggat tgagcgacaa acttggtact aaagctaagc cgatgttttg ttctaccgtt
     3901 aagtgcggga aaatattgtt atcttggaat agcattgaaa caggacgttg tgcaacttcg
     3961 gtatcaagat gatttttctg atttagccac agctggcttt gctgactttc tgccttttca
     4021 aaaccggcaa ttagattaag taatgtgctt ttgcctgcgc cactttcgcc aaccactgca
     4081 atacgttcgc cttttttgac agaaagctga aagtgcatat tcatttgttc gtaagtgaaa
     4141 tggagatcca gttcgatcat agtttatcct tgctgaataa gaatggtagt aacgcaaata
     4201 acatcagaat gagtgcggta acggcagcat cttcggttct atagctacca agttgttggt
     4261 agagtaaata agggagtgag ctgaaatcag cgttgccgaa aaaagcaatg acggtgaaac
     4321 tgcctaagct cgatgacatt gctaacgcaa atacggtaac aagcggtcga attagttgat
     4381 ttttttccac aatccaccaa cggcgaagcc cctttagccc aagtccttgt gctaatttgt
     4441 cgtgcgaggt gaggctattc cacattgcgg agaaaagcat tgggtaaata aatggcagta
     4501 atgttaagcc gttgcatatc ccgaccagta gcagtaatgc ctcgcttgag agttcactat
     4561 tcattaataa aataaacaaa ccaaccgata ataaaaagat cggcagaagt aaggggaata
     4621 gggtgattgc ggtgagaatg ctatatctga gcttggcttt tgcatagagt aattggcgag
     4681 cttcaagtgc aatcaaatag ctgattgtga ttacgaatat agaagcaatt aaacttaagt
     4741 ataaagaata ctgagtagct tgccataaag tagggttgag caaacgctca ctgagttttg
     4801 acacgcttag tcctgcccaa atgacgctga caagcggtag aattattgca aaactttgca
     4861 aaaaaataac cgcttgtaag ccgagtttag ccagtccggt aggctttggt ttccaaattt
     4921 ctactcggtt aggttgctgt tttagctttt tgagtgcaga agtcgagata taattcatta
     4981 ctaattgtaa gccaatgccc actacaaatt gtaccccaat taagatgagg gcttttgcaa
     5041 aatcaaattc aaaagtgacc gcttgataaa tggcaacttc aagcgtgctg tattttgggc
     5101 taccgcctaa cattaatacg attggaaaac tggtaaagca gaccagaaaa acagtcataa
     5161 acatataagg cagcaaagtt ttgattatcg gtagttccac aatttggata taattccaac
     5221 ctgttaggtt taattgagcc gcaagctgaa agcgactgct tggagtcaat tgaagccctc
     5281 cggtataata tttcatcaca aacggaatgt taaacaggca gtgagccaaa atgatgccgt
     5341 gcaagccgta taaattaaat tgccaattcc aaccgagttg ttggaaaagt ttggctagcc
     5401 agcctgagtt accaaatacg ccaattaccg caaaaataat tactagtgag ggtaatgccc
     5461 agataaacga aatggtttta tagagtaaat tttttccttt aaagtccaaa taaaagaaag
     5521 atcttgccag taggctgcct aaaaatagtg aaattaccgc agagaggctg gcttgaagta
     5581 ggctataagc gaccgtggcc gttatatcgg aaaaaacaaa taaggtatca ttttggtgat
     5641 agtccattaa cgcccataga ctaaaaacat agagagcaat aatactaaag tagataaacc
     5701 atgcggttgt tttggttaat gtgctaagca tattgctcca aatttagaat ataagtgaaa
     5761 gaagtggtag cgaaattatt gtaacatttc cgcttaagtg tgtgctagaa tcttgatatt
     5821 cgacaaatga tttttaagtt agcttaaaaa acaccctatt tagcagggtg atacgacgag
     5881 tttttgcgct ttttagcgtg tttttaattt gaatttgtag taaagttgcg tgcgcaaaat
     5941 aaatggatta aaacaaattt ttttaacttt caaatttaag aggaagattt tatgtactca
     6001 aaagatgttg ttattaccgc tccaaatggt ttacacactc gcccagcggc tgaatttgta
     6061 aaagcagcta aaggttttgc ttctgatatc actgtgactt caggtggtaa aagtgcaagt
     6121 gcaaaaagct tattcaaatt acaaacatta ggtttgactc aaggcacaac aattacaatt
     6181 tctgcggacg gcgaagatga gcaaaaagca gttgatttct tagttgattt gattcctact
     6241 ttagaataat ttttctattc tcggatttaa gatttatgtc actttattgt agcttaataa
     6301 gctacaataa agtgttttgc tatgtaacat ccagatgatt tttacatcat cttaaattaa
     6361 ggacacaatt atgattacag gtatcgcagc ttcccctggt gcagtatttg gaaaagcatt
     6421 ggttttaaaa gaagagccga ttgtactgaa tactcgcaaa attactgaaa gcgaagttga
     6481 ggcagaaaaa gcaaaattct ttacaggccg cgaaaaagca gctgctcaat taaccgcgat
     6541 taaagaaaaa gctcgccgta cattaggtga ggaaaaagag gcgattttcg aaggccactt
     6601 gatgatctta gaagatgaag agcttgaaga agaaattttg agttacctta ctgataatct
     6661 tgtgactgcg gatgttgcag caagtaaagt gattgagatg caggcttcaa tgcttgcgga
     6721 aattgacgat gaatatttaa aagaacgtgc aggtgatatt cgtgatattg gtaatcgctt
     6781 ggtacgcaat attttaaata tgaatatcgt agatcttggt gatattcaag atgaagttat
     6841 tttagtcgct tatgacttaa ccccttccga aacagctcaa ttaaatttag ataaagtatt
     6901 aggctttatt accgatatcg gtggtcgtac ttctcacact tctattatgg ctcgttccct
     6961 agagcttcct gccattgttg gtactaacaa tgttacttcc ttagttaaaa ctggtgattt
     7021 tctgatttta gatgcagtga acaatgaagt acatttaaat ccatcggaag aaaaaatcgc
     7081 taatttcaaa gcattacaag agaaattaaa tgcggaaaaa gaagaattag ccaaattaaa
     7141 agagttgcct gctcaaacat tagatggcca taaagttgat gtggcaggga atatcggtac
     7201 tatccgtgat gtggaaggtg tattacgcaa cggtggtgaa gcggttggtt tataccgtac
     7261 tgagtttttg tttatggatc gtactcaatt accgggagaa gaagagcaat ttcaagccta
     7321 caaagagatt gttgaagcaa tgaatggtca aatggtcatt ctgcgtacaa tggatatcgg
     7381 cggtgataaa gagttaccat accttgattt accgaaagag atgaacccgt tcttaggttg
     7441 gcgcgctgtg cgtatcggtt ttacccgccg tgaaatttta gatacccagt tacgagctgt
     7501 attgcgtgct tctgcgttcg gtaatttagc ggtaatgttc ccgatgatta tttcggtgga
     7561 agaaatccgt ttattaaaat ccatcgttgc ggaattaaaa gatcaattac gtgcagaagg
     7621 tcacgctttt gatgagaact taaaactagg tattatggtg gaaaccccat cagcagcggt
     7681 aaatgcacgt catcttgcaa aagaagcgga cttctttagt atcggcacta atgacttaac
     7741 tcaatatact ttagcggttg accgtggtaa tgaaattatt gctcatttat ataacccatt
     7801 aagcccatca gtcttaaact taatcaagca agttattgat gcttcacacg ctgaaggcaa
     7861 atggacaggt atgtgtggtg aattagccgg tgacgtgcgt gcaactgcgt tgttgctcgg
     7921 tatgggatta gatgaattta gtatgagtgg tatctctgtg ccacacgtta aaaaactcat
     7981 tcgctcaatt aattttgccg atgctaaagc attagctgat gaagcgcttg ctcaaccaac
     8041 tgctgcagat attgaacgct tagtggatga gttttatcag aaattaaact aattttaatt
     8101 atttagcata caaatacaaa attctcgtat aaaatgtaag agaattttgt atctaatatc
     8161 taagggagat tttaagatgg gtttatttga taaattattc ggctcaaaac aaaaagagag
     8221 tcaagaaatc aaaatctatg cgccattaac aggtgaaatc gtaaatattg aagatgtgcc
     8281 tgatgtagtt ttctctgaga aaatcgttgg tgatggtgtg gcaattcgcc cggttggcga
     8341 tacgattgtt tcgccggtaa atggaactat tggtaagatt tttgaaacaa accatgcttt
     8401 ctcaattgaa tctgaagaag gtgtagaatt atttgttcac tttggtattg atacggtgga
     8461 attaaaaggc gaaggtttta cacgtcttgc tcaggaaggt caaacggtaa aagtaggcga
     8521 tccaatcatc aaatttgatt tagctctact tgaaggtaaa gccaaatcag tattaacgcc
     8581 aatcgtgatt tcaaatatgg atgaaatcag caacttatct aaattaaatg gtcaagttgt
     8641 tgccggtgaa agcgtggttt taaccttaac caagtaatac cgcatactta tcttgaagcc
     8701 acgatagaag atcgtggctt tttgttttta agtaaaatca gataaatggc gattatttat
     8761 agataaaaaa taggcaacaa ggtaaaataa cgccataaat tttgcgtaaa gggtttattt
     8821 taagtcaaaa ataataaatt gctttacgct tttcttttct aatttattga attatttatt
     8881 atttttaaac gttatggcta aattacatat taccacttgg ggctgtcaaa tgaatgagta
     8941 cgactcatca aaaatggcgg atctcctaaa ctccactcac ggttttgaat tgaccgaaaa
     9001 tccagaagat gcggatgtac tgttgctaaa cacttgttct atccgtgaaa aagcacaaga
     9061 aaaagtattc tcgcaactcg ggcgttggaa aaactggaaa aaagataaac cgaacctgat
     9121 tatcggtgtt ggcggttgtg tggcttcaca agaaggggag catatccgtg atcgtgcccc
     9181 ttatgtagat attgttttcg ggccacaaac tttgcaccgt ttgcctgaaa tgatcaacca
     9241 aatccgtggc ggtaaaagct cgattgtcga tatttctttc ccggaaattg aaaaatttga
     9301 ccgcttgccg gagccaaaag ccgaaggccc tacagcattc gtttcaatta tggaaggctg
     9361 caataaatat tgttcgttct gcgttgtgcc ttacactcgt ggcgaagagg tttcccgccc
     9421 ggtggatgac gtattatttg aaatcgccca acttgcggca caaggtgtgc gtgaggttaa
     9481 tttactcggt caaaacgtaa atgcttaccg tggtgaaact tttgatggcg gcatttgtac
     9541 ctttgccgaa ttattacgct tagtggcagc gattgacggt attgaccgct tacgttacac
     9601 caccagccac ccgattgaat ttaccgatga tattatcgaa gtttatcgtg atacacctga
     9661 attggtcagt ttcttacatt taccgattca aagtggttca gaccgtgtat taacaatgat
     9721 gaagcgtaac cacacagcgt tggaatacaa agcgattatc cgtaaattgc gtgctgttcg
     9781 acctgagatt caaattagtt cggactttat tgtaggcttc ccgggcgaaa ccgcagagga
     9841 tttcgagcaa acaatgaaag tgatcgagca agtgaacttt gatatgagct ttagctttat
     9901 ctactcggct cgaccgggta caccagcggc agatctgcct gataatgtct ccgatgatga
     9961 gaaaaaagag cgattatccc gtttacaaca acgcatcaac caccaagcaa tgcaatttag
    10021 ccgtgcaatg ctcggtacag aacaacgcat tttggttgaa ggcccgtcta agaaagactt
    10081 gatggaatta accggcagaa ccgaaactaa ccgtgtagta aactttgttg gcacaccgga
    10141 tatgattggc aaatttgtcg atgtgaaaat caccgatgtt tataccaact cactccgtgg
    10201 cgaagtgatt cgtaccgaag accaaatggg attacgtgtg gtggaatcgg cagaaagcgt
    10261 gattgcccgc actcgtaaag aagatgattt aggtgtaggc aaatacgtgg ttaatctgta
    10321 aaaattaaag aaaaaatgac cgcttgtagg tatgtacaag cggtctttta gtatataatc
    10381 gaataaatgc taaatcgttt tagtttggtg ttttgtgact aattaaaggt gtatgagatg
    10441 agtaagattt gggtaacagg cgatgcggtg gtggatttaa ttcccgacgg cgaacatcac
    10501 tatttaaaat gtgccggtgg tgctccggcg aatgttgcag taggcgtttc tcgtttaggg
    10561 gttgaggcag gatttatcgg gcgtgtgggt aacgaccctc tgggtaaatt tatgcgagaa
    10621 gtgttacagg cagaaaatgt ttgcaccaaa cagatgattt tagacgctca gcaccgcact
    10681 tccaccgtga ttgttggctt agataacggt gagcgtagtt ttacttttat ggtaaatccg
    10741 agtgccgacc aatttttaga aatcggcgat ttacctgaat ttaaccaagg tgatttctta
    10801 cactgctgtt ctattgcctt gattaacgac ccatcccgtt ccactaccat tgaagggatt
    10861 cgccgagtga aaaaggcggg tggctatgtt tcgtttgatc caaacttgcg tgaatcgctc
    10921 tggaagagcc ttgatgaaat gaaaacagtg gtaaattctg tggtggcgat ggcggatatt
    10981 ctcaaatttt ccgaagaaga gctgacctta ttaaccgaga cagaaacttt ggaacaagcc
    11041 acacaagcta ttaccgctca gtatccggag aaattgatta ttatcaccct tggcaaagac
    11101 ggtgcaatct accatttcaa cggaaaaagc caagtcgttg ccggcaaagc cttgaaacct
    11161 gtggatacca ccggtgccgg agatgccttt gtcagcggat tattagccgg tcttgctcaa
    11221 acgccgaatt ggcacgatga aactgtgtta gttgaagtga tccgcaaagc aaatgcttcc
    11281 ggtgcgttag ccaccacagc aaaaggggcg atgtcggcat taccgaataa agcggagtta
    11341 gacgcatttt tggcggaata atgtaatgat ggcgcaagtg tccacgcttg tgcctcattt
    11401 tttacaaaag ggtacaagcg tggacgcttg caccatcgta atttctgaaa tgacaggaac
    11461 actctatgca cattttcaac aacggcaaat acaaaagcct gcacgcacaa agcgagggcg
    11521 aattaaacgc tcttcgccaa accgttttat ctgatcccga tttttaccca acctatcatc
    11581 tcgctcccga aacaggctta tttaatgacc caaacggctt gattttcgat ggtgaaaaat
    11641 accatatttt cgcccaatgg tttccttatg gggcattaca tggaatgaaa cattggcagc
    11701 atttcatcac taccgatttt caaacgttca gtaaagggga tttgctgatt cccgatgagc
    11761 tgtttgaatc ccacggctgt tattcaggtg gggcgatttt atggcaagat aagattgtcg
    11821 ctttttacac cggcaataca cgccgcccat cagataatgc ccgcattcct caccaaaata
    11881 tcgcgatttt tgataaatcg ggcaaattat tggaaaagcg ttgcattatc gaccaagcac
    11941 cggcaggcta caccgaacac gtgcgtgacc cgaaacctta ccttaccgca gagggcaaaa
    12001 ttcgttttgt gttgggggct cagcgtgaaa acttgaccgg cacggcgtta atttatgaaa
    12061 tggacgattt aaatgccaca ccccgtttaa tcgcagaact ggaagtccac aattttgata
    12121 acagcaatgt gtttatgtgg gagtgtccgg atctgttctc acttggtggt aaagatctgt
    12181 ttgtgtggtc gccacaaggc aaactgcgtg aaagccatca attccaaaat aactaccacg
    12241 ccacttacgc acttggcgag ctaaacggca acagattacg ggcagagcat attgaagagc
    12301 tggactacgg ctttgacttt tacgcaccgc aaacggtgga aaa
//
LOCUS       DANJWC010000065        11965 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.065, whole genome shotgun sequence.
ACCESSION   DANJWC010000065 DANJWC010000000
VERSION     DANJWC010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11965
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.065"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..458)
                     /locus_tag="PWX02_001293"
     CDS             complement(<1..458)
                     /locus_tag="PWX02_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729308.1"
                     /translation="MADPHIQSPMDFWDCLTVIIYRSGFVLATLMLLLLPYYPQTAQY
                     GLLVSGTMLASSLHLYAKSFRLIFQFSAWIGLLGAVFNFPLLAFGAMLLVIGGLSYKE
                     YFCFRVFGLNFQPLLVAVLWIAFALEWSLLVQILSLVCGLLLLVLSIQKWR"
     gene            complement(481..1458)
                     /gene="dusA"
                     /locus_tag="PWX02_001294"
     CDS             complement(481..1458)
                     /gene="dusA"
                     /locus_tag="PWX02_001294"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HDL1729309.1"
                     /translation="MTNEIQPFYRGRFSVAPMLDWTTRHCRYFHRQFSRQALLYTEMI
                     TAPAIIHAKYDLLEYDPSENPVALQLGGSDPAQLAHCAKLVAERGYAEINLNVGCPSD
                     RVQNGMFGACLMAKADLVADCIKAMQDAVEIPVTVKHRIGIDDLDSYEFLCDFIEKVQ
                     PYSNDFIVHARKAWLTGLSPKENREIPPLDYDRVYQLKRDFPHLKISINGGIKTIEEI
                     KQHLQFVDGVMVGREAYQNPSMLGEIDSQIFNENRPLVTAKEAVEKMFPYIERELAKG
                     VYLNHIVRHMLGAFQHCKGARQWRRHLSENATKPGAGVEVVEQALAFIG"
     gene            1809..2786
                     /locus_tag="PWX02_001295"
     CDS             1809..2786
                     /locus_tag="PWX02_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDL1729310.1"
                     /translation="MSRNLFQKRLNIKPYEYPELLEFKDAIRHSYWLHTEFNFTGDIQ
                     DYRTTINDHERHVLTRAMLAISQVEVNVKRFWGELYRYFPKPEMDDVGGTFAESEVRH
                     KDAYSFLLEKLGLNEMFSQIHEIEPLMNRIRYMESFMKDKDAGKGEFVLSLVLFSLFV
                     EHISLFGQFIIMMSFNKHKNLFKGISNAVEATSKEEEIHGKFGIALYEILRDEHSELF
                     TPEFFEDLQTLANQAFEAERGILDWIFEEGDLSFITKATVENYIKNRYNNSLSILGLE
                     PPHDIDPEFLKETEWFDIEILSTKETDFFNKRSTDYSKKMKQITADDLF"
     gene            2773..4440
                     /locus_tag="PWX02_001296"
     CDS             2773..4440
                     /locus_tag="PWX02_001296"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDL1729311.1"
                     /translation="MTYFNTDRPDFHWLNEDSRLFLQRGYLLEGTTALDRIRFIAEHA
                     ERKLGIEGYADKFYHYMARGYFSLSSPIWSNFGLDRGLPISCFGSYIGDSIHEIMVTT
                     AEVGMMSKIGGGTSAYFGDIRPRGSLIKNNGKSDGSFNFSKLFDTVIDVISQGTSRKG
                     QFAGYIDIEHGDIDEWLDIHTEGNPIQLMYYGVCVGHDWLESMKAGDPYKRQLWAKLL
                     QRKTETGIPYLFFKDNANAGRPDVYKDKNMTIHASNLCTEIMLPSSNEESFVCCLSSM
                     NLLYFDEWKDTDAPETLTYFLDVVMSEFIEKSQSIPFLDRANRFARRHRALGLGVLGW
                     HSYLQANNIAFDSFQAMQKNNEIFKTLQAKTLKASQELAQRFGEPEILEGYGRRNTTL
                     MSIAPTKSSSFILGSVSPSVEPFKSNYYVKDLAKIKTVYKNPFLEKLLQEKGLDTEEI
                     WTSILQNDGSVQHLEQLSEHEKEVFKTFAEISQLSVIQQAAQRQKYIDQGQSINVMIH
                     PATPARDLNQLYLTAEELGLKSIYYQNSMSAAQVFNRNLLSCSSCEG"
     gene            4444..4863
                     /locus_tag="PWX02_001297"
     CDS             4444..4863
                     /locus_tag="PWX02_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729312.1"
                     /translation="MGNLSHTSPNTIVEVVGEFPEITSSGYTFDKGPIQLRIGKHTAP
                     NKGFGVKHIEAEHMQEILNLGFTSVNEFVKAIIVENAKIFCEFSDLKGNHRPIILKAS
                     IGIVVLERKFLESGTIYSVVTAYRRTNPHGIQIGTMK"
     gene            complement(5098..5859)
                     /gene="nudC"
                     /locus_tag="PWX02_001298"
     CDS             complement(5098..5859)
                     /gene="nudC"
                     /locus_tag="PWX02_001298"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HDL1729313.1"
                     /translation="MNKGYWIIVSDFDIALEAGEIPFGSAEELGLSGREKLTIGQYQG
                     SPAYLVKALESDNQREFAYLRSQINRPPELSHLLHRAVSLNHFFQTHQFCGKCGEKTI
                     LASDEIAIYCNACGNRAYPTISPSIIVAVRKGRQILLANHLRHKGTIYTTLAGFVEAG
                     EAVETAVHREVFEETGIKVKNVRYFGSQPWAFPNSLMLGFLADYESGEIQLQEEEICD
                     AKWFDCEESLPEIPQQGTIAFELIQATLKLCRESK"
     gene            complement(5852..6535)
                     /locus_tag="PWX02_001299"
     CDS             complement(5852..6535)
                     /locus_tag="PWX02_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HDL1729314.1"
                     /translation="MKQPTFKRMTNKRSNFAKNLQKNPQKRPLTFAETTLVLLNKPYD
                     VLTQFTDEQGRKTLKDFITMPNIYPVGRLDRDSEGLLLLTNNGEIQHRLAHPKFDKQK
                     TYWVQVEGVPQPEDLAKLAKGVELKDGLTKPAKVRAIATPDFAWHIAPQIRERKTIPT
                     SWIELIISEGKNRQVRRMTAHIGFPTLRLIRVGIGQFRLGNLGCGEFQVLSENEKRKL
                     FTQLGLKDE"
     gene            complement(6532..7380)
                     /locus_tag="PWX02_001300"
     CDS             complement(6532..7380)
                     /locus_tag="PWX02_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL1729315.1"
                     /translation="MEKRTLALLKVHGTAVLFGASVIFGALIQSSAETIVLGRVMIAF
                     AIISLYFLWKKQPLVKLNLQQMAQLAFSAALLAAHWVTFFVAVKVGGVAVATLGFASF
                     PAFVALFESLFFREKLRFREVVLLIAITVGLILVTPEFTFGNQATQGLLWGVLSALVY
                     GILAVANRRTMSKISGVQASWWQYIFATLFLLPLSITEFPSVSTQDWFWIFCIGFLCT
                     TLAYTLFISSLDTINARTASMIISLEPVYAIVIAWLCFNEVPTLKMIIGGLIILLSVA
                     WANLKK"
     gene            complement(7380..7865)
                     /locus_tag="PWX02_001301"
     CDS             complement(7380..7865)
                     /locus_tag="PWX02_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HDL1729316.1"
                     /translation="MPILAILFGIFFYIYCEISLLVNIGSIIGVLPTILLLVVISFLG
                     LWIVKLRGVYTLFSIKQDLSQGKIPTEAVGNSVMFIVAGILLIIPGFLSDILAILCVL
                     PFSRKWIQAFVINSVKNKVFMHFTAQTSSFSATSAQDSNTFDAEFERKPDEQDQHNRI
                     K"
     gene            complement(7867..9189)
                     /gene="pepQ"
                     /locus_tag="PWX02_001302"
     CDS             complement(7867..9189)
                     /gene="pepQ"
                     /locus_tag="PWX02_001302"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HDL1729317.1"
                     /translation="MKNLFSTHLARLQQTVQSLLENAFLDGLWIYAGKAKTLFLDDQT
                     VPFKINPYFNYFFPYPQAENCWLFLDGKNKPKIYFYAPEDYWVSTLKVPTEAFFAAEF
                     EWVLLRAENEIATFIQNPTACAFIGEDVELAKKLGFSHINPQKVLNSLNFARSIKTEF
                     EIESIYQAQFSALKGHQVAKEAFFVGKSEFEINLEYLKATQQSDLNVPYGNIVAINQN
                     GAVLHYTALDFDTHAKRHSFLIDAGTTVNGYASDLTRTYAFDSGSEFAELVAKMEQLK
                     LQIIAELSIGVNYLHYHTRMQQMISQLLAEHQFIHLSAEQIFEQGISRTFFPHGLGHL
                     LGIQVHDVGGFQQNARGSHKAPPEVYPSLRCTRELQENMVLTIEPGFYFIEMLLKPWK
                     NSALSSAFNWQKIEAFKAFGGIRTEDNIVMRANGAENLTANAAATLGG"
     gene            9200..9442
                     /gene="tusA"
                     /locus_tag="PWX02_001303"
     CDS             9200..9442
                     /gene="tusA"
                     /locus_tag="PWX02_001303"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HDL1729318.1"
                     /translation="MTQLTVNKELDTLGLRCPEPVMLTRKTIRNMEEGEILHIIADDP
                     ATTRDIPSFCEFMDHQLIQKEVDSTPYHYWIKKGLE"
     gene            complement(9509..9940)
                     /gene="rraB"
                     /locus_tag="PWX02_001304"
     CDS             complement(9509..9940)
                     /gene="rraB"
                     /locus_tag="PWX02_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HDL1729319.1"
                     /translation="MYNLDELRTETDETISSLLADGSDPEALYIIEHHVAHHDFDKLE
                     KLVVDAYKLGYEISEAEEVEDENGKVIFVCDIVSEIHLNADLITAQQKELLPLIEKAG
                     AEYEGWGTYFEDPNADEDEYGDDGEFFDDENDDFEDEPRRH"
     gene            complement(9955..10566)
                     /locus_tag="PWX02_001305"
     CDS             complement(9955..10566)
                     /locus_tag="PWX02_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDL1729320.1"
                     /translation="MKLNYHKTHFLTSAPDIRHLPEDTGVEIAFAGRSNAGKSTALNA
                     LTNQKSLARTSKTPGRTQLINLFEVETNCKLVDLPGYGYAAVPEQMKIQWQKALGEYL
                     QKRECLGGVVILMDIRHPLKDLDQQMIEWAVSANLPVLLLLTKADKLSQSARSKQVKM
                     VREAILPFQGDIQVEAYSALNKIGIDKLSNKLDSWFAPKLITE"
     gene            complement(10568..10984)
                     /locus_tag="PWX02_001306"
     CDS             complement(10568..10984)
                     /locus_tag="PWX02_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDL1729321.1"
                     /translation="MSWYKALFSFNGRLNRRGFWIGFGIHFLFLFMVAMWINLAELSL
                     LSVLPLIITGFSLSAIIIKRLHDRNRSAKALLMALVPIICYLAAPYSQGIMQILLGQV
                     MPAFIGMLLFLEWGVFAGNPEPNQYGEQGLSFKFRE"
     gene            complement(10988..11818)
                     /locus_tag="PWX02_001307"
     CDS             complement(10988..11818)
                     /locus_tag="PWX02_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDL1729322.1"
                     /translation="MFEYFLSLSPIWQAFLAGLFTWGCTIFGSSFVYFFKSVNRKLLD
                     SMMGFAAGVMIAASFWSLLAPSLEYAEADYGSLAWLPAAIGFLAGGAFIRLIDYVVPH
                     LHLSKPIEQAEGNDQLKQGLSKSTLLFLAITIHNIPEGLAIGVAFGALATQVSGVDAS
                     IMGAIGLAIGIGLQNIPEGSSLALPIRGEGHSRKKAFWYGSMSAVVEPIAAVIGAVFV
                     LSMTAILPYALAFAAGAMIFVVVEELIPESQSSGNTDIATLSLMLGFVVMMVLDVTLG
                     "
BASE COUNT         3767 a         2636 c         2262 g         3300 t
ORIGIN      
        1 cgccattttt gaatactcag cacaagaagc aacaagccac agacaaggct taaaatttgc
       61 accaataaag accattctag tgcaaaagca atccacaata ccgccactaa aagcggttgg
      121 aaatttaagc cgaatactct aaaacagaaa tattctttat agcttaatcc gccgatcacc
      181 agtagcattg ccccgaacgc aagcaaaggg aaattaaaca cagcaccaag taagccaatc
      241 caagcagaaa attggaaaat caggcgaaac gatttggcat aaagatgcaa agaagatgcc
      301 aacattgtgc cagatactaa taaaccatat tgtgccgttt gcggataata aggcaataaa
      361 agcaacatca atgtagctaa cacaaaaccg ctgcggtaaa tgatgacggt taaacaatcc
      421 caaaaatcca ttggcgattg aatatgtgga tctgccataa ctgcctcctt aaacaataaa
      481 ttagccaata aacgcaaggg cttgttccac tacttctaca ccggctcccg gcttagtggc
      541 attttcactt aaatgacgac gccactgcct cgctcctttg caatgttgaa acgcaccaag
      601 catatggcga acgatatgat ttaaatagac acctttggca agctcacgtt caatataagg
      661 gaacatcttt tccaccgctt cttttgccgt tacaagcggc cgattttcat taaaaatttg
      721 cgaatcgatc tcgcctagca ttgacgggtt ttgataggcc tcacgcccta ccattacccc
      781 atcaacaaac tgcaaatgct gtttaatttc ctcaatggtt ttaatgccgc cattgatgct
      841 aatctttaaa tgcggaaaat ctcgcttgag ctgataaacc cgatcataat ctaaaggcgg
      901 aatttcacgg ttttccttcg ggcttaaccc tgtcagccac gccttgcggg cgtgtacgat
      961 aaaatcattg ctataaggtt gcactttttc gataaaatca cacaaaaatt catagctgtc
     1021 taaatcatca atcccaatac gatgtttaac cgtgaccgga atttccaccg catcttgcat
     1081 tgctttaata caatccgcca ctaaatctgc ttttgccatc aaacaagccc caaacatacc
     1141 gttttgcact cggtctgacg ggcaaccgac atttagatta atctcggcat agcctcgctc
     1201 tgccaccaat ttggcacaat gagccaactg tgccgggtcg cttccaccca gttgcaaagc
     1261 caccggattc tcgctcggat catattccaa caaatcatat ttggcgtgaa taatcgccgg
     1321 ggcggtgatc atttcggtat aaagcaaggc ttgacgacta aattggcgat gaaaatagcg
     1381 gcagtgacgg gttgtccaat ctaacattgg agcaaccgaa aaacgtccac ggtaaaaagg
     1441 ttgaatttcg ttagtcataa aaatatcaaa ataaagcggt aaaaaatcag taaaaaataa
     1501 ccgcttgtaa gcgattcaaa acaagcagcc atcatactcg aaaaagccag ataaacatag
     1561 tttcctcaag gcttccccat ctatctgtgc acttttaaaa caaccttaaa acacaatata
     1621 tagtgtttca acatcaaatc acacacaaca tattgaagca aaaatttaca aaattatttt
     1681 tctttataaa tcaataagct accaaagacc aataaaaaag agtgtttgtc aagacttgtc
     1741 ttgaaacttt ttttgcatac aatcatctac cagttccaaa cataaacttt aagatagagc
     1801 ctacgaatat gtctagaaac ctcttccaaa aacgcttaaa tattaagccg tatgagtatc
     1861 ctgagcttct tgaatttaaa gatgcaattc gccattctta ctggttacac accgaattta
     1921 acttcaccgg cgatattcaa gactatcgca ccaccattaa cgatcacgaa cgtcacgtgt
     1981 taacccgagc aatgcttgcg atttcacaag tagaagtgaa tgtaaaacgt ttctggggcg
     2041 agctataccg ctatttccca aaaccggaga tggacgatgt aggcggtact tttgctgaaa
     2101 gtgaagttcg ccataaagat gcttactcgt tcctattgga aaaattaggc ctaaatgaaa
     2161 tgttcagtca aattcatgaa attgaaccat taatgaaccg cattcgttat atggaaagtt
     2221 ttatgaaaga taaagatgct ggcaaaggcg aatttgtttt atcgttagtg ctgttctctt
     2281 tatttgttga gcatatttca ttgtttggac agtttattat catgatgtcg ttcaacaagc
     2341 ataaaaacct gtttaaaggg atttccaatg cggttgaagc cacctcaaaa gaggaagaaa
     2401 ttcacggtaa attcggaatt gcgttatacg aaattttacg tgacgaacac agtgaactat
     2461 tcacaccaga attttttgaa gatttgcaaa ccttggcaaa tcaagcgttt gaagcagaac
     2521 gtggcatctt agactggatt ttcgaagaag gtgatttaag ctttatcacc aaagcgacgg
     2581 tagaaaatta cattaaaaac cgttacaaca attctttaag cattttaggg ttagagccgc
     2641 cacacgacat cgaccctgaa ttcttaaaag agactgagtg gtttgatatt gaaattcttt
     2701 caaccaaaga aaccgatttc tttaacaaac gtagcaccga ttattctaaa aaaatgaaac
     2761 agattaccgc agatgactta ttttaataca gaccgcccgg acttccactg gcttaatgaa
     2821 gacagccgtt tattcttaca acgtggctat ttattggaag gaacaaccgc attagaccgc
     2881 attcgtttta ttgccgaaca cgcagagcga aaactcggca ttgaaggcta tgccgataaa
     2941 ttctatcact atatggcaag aggctacttc tcgctttctt cgccaatttg gtcaaacttt
     3001 ggcttagatc gtggcttacc gatttcttgt ttcggtagct acatcggcga ctctatccat
     3061 gaaattatgg tgactaccgc tgaagtgggc atgatgagta aaattggcgg tggtacttcg
     3121 gcctactttg gcgatattcg tccgcgtggc tcgttaatta aaaacaacgg taaatcagac
     3181 ggctcattta acttcagtaa attatttgac actgtgattg acgtgatttc acaaggaaca
     3241 tcacgcaaag gtcagtttgc cggttatatt gatattgagc acggcgatat tgacgaatgg
     3301 ctggatattc acaccgaagg caacccaatt caattaatgt attacggtgt gtgtgtcggt
     3361 cacgattggt tagagtcaat gaaagccggc gatccataca aacgtcagct ttgggcaaaa
     3421 ttgttacaac gtaaaaccga aaccggtatc ccttatttat tctttaaaga caatgccaac
     3481 gcaggtcgtc ctgatgtata taaagataaa aatatgacga ttcacgcctc taacttatgt
     3541 actgaaatta tgttgccttc cagcaacgaa gaaagtttcg tatgctgctt atcgtctatg
     3601 aacctactct actttgatga gtggaaagat accgatgcac cagaaacatt aacctatttc
     3661 ctcgatgttg taatgagtga atttattgag aaaagtcaat caattccatt cttagaccgt
     3721 gcaaaccgct ttgcccgtcg ccatcgtgca ttaggtttag gcgtgttggg ctggcacagc
     3781 tatctacagg caaataatat tgcctttgac agcttccaag cgatgcaaaa aaacaacgag
     3841 attttcaaaa cgcttcaagc aaaaaccttg aaagcctcgc aagaattggc tcaacgcttc
     3901 ggcgagccgg aaattttaga gggctatggt cgccgtaata ctacattaat gagtattgct
     3961 ccgactaaat ccagtagctt tattttaggc agtgtgtcgc catcggtaga gccgtttaaa
     4021 tccaactact atgtaaaaga tttagcgaag atcaaaaccg tgtataaaaa cccgttctta
     4081 gagaaattac tccaagaaaa agggctagac accgaagaaa tttggacgtc tattttacaa
     4141 aatgatggct cggttcagca tttggaacag ctttccgagc acgaaaaaga agtatttaaa
     4201 acctttgccg aaatcagcca attaagcgtg attcagcaag ctgctcaacg tcaaaaatat
     4261 attgaccaag ggcaaagtat taatgtgatg atccacccag ccacaccggc tcgtgatctt
     4321 aaccaattat acttaacagc agaagagctg ggcttaaaat ccatctacta ccaaaactca
     4381 atgagtgcag cacaggtatt taaccgtaac ctattaagct gctcaagttg cgaagggtaa
     4441 ataatgggaa atttatcgca tacttcacct aatactattg ttgaagttgt tggtgaattt
     4501 cctgaaataa catctagtgg gtataccttt gataaaggtc ctattcaatt aagaatcggc
     4561 aaacatactg ctccaaataa aggctttggt gtaaagcata ttgaagccga gcatatgcaa
     4621 gaaattctta atctaggatt tacatcagtt aatgaatttg tcaaagctat catagtagaa
     4681 aacgctaaaa ttttttgcga attctcagat ttaaaaggca atcatcgccc tattattctg
     4741 aaagcaagta ttggcattgt ggttctagag cgtaagtttt tggaaagcgg tacgatatat
     4801 tcagttgtaa ccgcttatag aagaacaaat ccccacggaa tacaaatagg cactatgaaa
     4861 taaagtacgt tagtgttata atcagttcca cgcaagagca tttgtagaaa ataacaggcg
     4921 gatttggttt tctactgtgg gcggcaggtt tccgctaacc tgtaatagct atttaatttt
     4981 tatattaaat cctgaacttg aaacagacta agggattgct gcggtagcta agaatacaaa
     5041 cctctctata atcatataga gaggtttttt cttattgatg agcacaagtt aagataatta
     5101 tttactctcc cgacataact tcaaggtagc ttgaatcagc tcaaaagcta ttgtgccttg
     5161 ttgtgggatt tcaggtaagc tttcctcaca gtcgaaccat tttgcatcgc agatttcttc
     5221 ttcctgtagc tgaatttcac cgctttcata atccgctaaa aaacctagca ttaatgaatt
     5281 gggaaacgcc caaggttggc ttccaaaata acgcacattt ttcactttaa tgcccgtttc
     5341 ttcaaacact tcacgatgca ccgcagtttc taccgcctca ccggcttcga caaaaccggc
     5401 taaagtggta taaattgtgc ctttatggcg tagatgattg gctagtagga tttgtctgcc
     5461 ttttcgcacc gccacaataa ttgacgggct aatcgttggg taagctcgat tgccacacgc
     5521 attgcagtaa attgcaattt catcgcttgc cagtatggtt ttctcgccac attttccgca
     5581 aaattggtga gtctgaaaga aatggttaag cgaaacagct cgatgcaaca aatgagacag
     5641 ttcgggtggt cggttaattt ggcttcttaa ataagcaaac tctcgctgat tatccgattc
     5701 taacgccttc accaaataag ccggcgagcc ttgatattgc ccgatggtta atttctcacg
     5761 tccacttaag cccagctctt ccgcacttcc gaatggaatt tccccagcct ccaaggcaat
     5821 atcaaaatcg ctgacgataa tccaatatcc tttattcatc tttcaatcct aattgtgtaa
     5881 acaacttacg tttttcattt tcagatagca cttgaaactc accacagcct aaattgccta
     5941 atctaaactg gcctatccca acacgaatta accgtaaagt cgggaaaccg atatgtgcag
     6001 tcattcttct gacttggcga tttttccctt cagaaataat caattctatc cagctggtcg
     6061 gaatggtttt ccgctcacga atttgcgggg cgatatgcca agcaaaatcg ggcgttgcta
     6121 ttgctctgac tttcgcgggc ttggtcaaac catcttttaa ctccacccct tttgctaatt
     6181 tggcaagatc ttccggttgt ggaacaccct ccacttgtac ccaataggtt ttctgcttgt
     6241 caaatttcgg gtgagctaaa cggtgctgga tttcgccatt attagtcaaa agcaacaatc
     6301 cttcactgtc tctatctagc cgcccgaccg ggtaaatatt gggcatcgta ataaaatctt
     6361 ttagcgtttt tcgcccctgt tcatcggtaa attgggttag tacatcataa ggcttattga
     6421 gtaacacaag ggtggtttcc gcaaaggtaa gcggtcgttt ttgcggattt ttttgcaaat
     6481 ttttagcgaa atttgaccgc ttgtttgtca ttcttttaaa ggttggttgt ttcatttttt
     6541 caggttcgcc cacgcaacag aaagtaaaat aattaagcca ccgataatca ttttaagggt
     6601 tggcacttca ttaaagcaaa gccacgcgat cacaatcgca tacaccggct cgagggaaat
     6661 aatcattgag gcagtacggg cgttgatggt atcaaggctt gaaataaaca gcgtataggc
     6721 aagggttgta cataaaaaac cgatacagaa aatccaaaac caatcttggg tggaaacgct
     6781 tggaaattca gtgatggaga gaggcaacag aaaaagtgta gcaaaaatat actgccacca
     6841 gcttgcttgc acaccggaaa ttttgctcat ggtacggcga ttcgctaccg ccaaaatacc
     6901 atacaccaaa gcagataaca cgccccacaa taaaccttga gtggcttgat taccaaaagt
     6961 aaattcaggg gtaactaaaa ttaaaccaac cgtgatggca attaatagca ccacttcacg
     7021 aaagcgtaat ttttcacgga agaacaggct ttcaaataac gccacaaatg ccggaaagct
     7081 ggcaaagcct aaggttgcta ccgccacacc acctacttta accgccacaa aaaaagtaac
     7141 ccagtgggca gctaataaag ctgcagaaaa tgcaagttga gccatttgct gtaagttgag
     7201 ctttacaagc ggttgttttt tccataaaaa atacaaagag atgatagcaa aagcaatcat
     7261 cactcgccca agcacaatgg tttcagccga gctttgaatt aatgctccga agatcaccga
     7321 ggcaccaaat aaaaccgctg tgccgtgtac tttaagtagt gcaagtgttc ttttctccac
     7381 tatttgatcc tattatgttg atcttgctcg tccggcttgc gttcaaattc tgcatcaaac
     7441 gtgttgctat cttgcgcaga tgtagcagaa aaagacgaag tttgggcggt aaaatgcata
     7501 aacaccttat tctttacgct attaattacg aaggcttgaa tccattttcg gctaaagggt
     7561 aacacgcata aaatcgctaa aatatcactt aaaaagccgg gaataatcag caaaatgccg
     7621 gctacaataa acatcaccga gttgccgact gcttcagtcg gtatcttgcc ttggcttaaa
     7681 tcttgcttga tggaaaagag ggtataaacg ccccttaact tcacaatcca taaccctaaa
     7741 aatgaaatca cgaccagtaa caaaatcgtg ggtaacacgc caatgatcga gccaatattc
     7801 accagcagcg aaatttcgca atagatataa aagaaaatac caaacagaat cgctaaaata
     7861 ggcatattat cctcccaacg ttgcggcagc gtttgccgtc agattttctg ccccattggc
     7921 tcgcatgaca atattgtctt cggtacgaat cccgccaaag gctttgaaag cctcaatttt
     7981 ctgccagtta aaggcagaag ataaggcact gtttttccaa ggcttaagta gcatctcaat
     8041 aaaataaaaa cccggctcaa tggtaagcac catattttcc tgtagctcac gggtacaacg
     8101 caaacttggg taaacctctg gaggagcttt atggctgcct cgggcatttt gctgaaaacc
     8161 gcccacatcg tgcacttgaa tacccaataa atgccctagt ccgtgtggga agaacgtacg
     8221 gctaatccct tgttcaaaaa tttgctcggc tgaaagatga ataaattgat gttccgctaa
     8281 taattgcgaa atcatctgct gcattcgggt gtgataatgc aggtagttca ccccaatact
     8341 cagctcagca ataatctgca acttcaactg ttccattttc gccaccagct cggcaaattc
     8401 agaaccgctg tcaaaggcat aggtgcgggt gagatcggag gcataaccat ttaccgttgt
     8461 gccggcatca attaaaaaac tgtggcgttt tgcgtgggtg tcaaaatcta acgccgtata
     8521 atgtagcacc gcaccatttt gattaatcgc cacaatattg ccataaggaa cattgagatc
     8581 agattgctgc gtcgctttca aatattcaag gttgatttca aattcgcttt tgccgacaaa
     8641 aaatgcctct tttgctactt gatgcccttt taaggctgaa aattgggctt ggtaaatact
     8701 ttcaatttca aactccgttt tgatggagcg ggcaaaattc aggctattca gcactttctg
     8761 cggattaata tggctaaagc ctaacttttt ggctagctca acatcttcac cgataaaggc
     8821 acaagcggtc ggattttgga taaaggttgc aatttcattc tctgctctca acaaaaccca
     8881 ctcaaattcc gcagcgaaaa aggcctccgt tggcactttt agagtggaaa cccaataatc
     8941 ttcaggggca taaaaataga ttttcggctt atttttgcca tctaaaaata accagcaatt
     9001 ttcagcttgg ggatatggga aaaagtaatt aaaataaggg ttaattttaa acgggacggt
     9061 ttgatcgtct aaaaataagg tttttgcctt gccggcataa atccataaac cgtctaaaaa
     9121 ggcattttcc agcaagcttt gcactgtctg ttgcaaacgt gcgagatgcg tagagaatag
     9181 atttttcata aggaataaaa tgactcaatt aactgtaaat aaagaattag ataccttggg
     9241 gctacgttgc ccagagccgg tgatgctcac ccgtaaaaca atccgtaata tggaagaggg
     9301 agaaattttg catatcatcg ccgatgaccc ggccactacc cgtgatattc cgagtttctg
     9361 cgaatttatg gatcaccaat taattcaaaa agaggtggac tccaccccat accattactg
     9421 gattaaaaaa ggcttggaat aaaccacaag cggtcgattt tagccataaa attgcaaatt
     9481 tatgcacaaa atcgaccgct tgttttaatt aatgacggcg aggttcgtcc tcaaaatcgt
     9541 cattttcatc atcgaaaaat tcaccgtcat cgccgtactc atcttcatca gcattcggat
     9601 cttcaaagta ggttccccag ccctcatact ccgcaccggc tttttcaatt aatggcagta
     9661 actctttttg ttgtgcggta atgagatccg cattcaagtg aatttcgctc acaatgtcgc
     9721 atacaaaaat cactttacca ttttcgtctt ccacttcttc cgcttcggaa atttcatagc
     9781 ccagcttata ggcatcaact actaattttt ccaatttatc aaaatcgtgg tgagcaacgt
     9841 ggtgttcaat aatataaagg gcttcagggt ctgaaccatc ggctaataat gatgaaatgg
     9901 tttcatcggt ttctgtgcgt aattcatcga gattgtacat attgaattcc ttacttattc
     9961 tgtgattaat tttggtgcaa accagctatc taatttgttc gacagcttat caataccgat
    10021 tttattcagt gcggaataag cctctacttg gatatcacct tggaatggta aaatcgcttc
    10081 tcttaccatt tttacctgct tagagcgtgc cgattggctt aacttatccg ctttggtcaa
    10141 cagcagtaac accggcaagt tggctgacac tgcccattca atcatctgtt gatctaaatc
    10201 tttcaacggg tggcgaatat ccattaaaat caccacaccg cctaaacatt cacgcttttg
    10261 taaatactcg cctaatgctt tttgccactg gattttcatc tgttcaggta ctgctgcata
    10321 gccatagcca ggtaaatcca ctaatttaca attcgtttca acctcaaaca agttaatcaa
    10381 ctgcgttcgc cccggtgttt tggaagttcg ggctaaactt ttttggttag ttaaagcgtt
    10441 caaagcagtc gatttaccgg catttgaacg cccggcaaaa gcaatctcca cacctgtatc
    10501 ttcaggtaaa tgacgaatat ccggtgctga agttaaaaaa tgggttttat gatagtttaa
    10561 tttcatttta ttctctaaat ttaaaagata agccttgctc gccatattga ttcggttcag
    10621 gattaccggc aaatacaccc cattctaaaa agagcaacat tccaataaat gccggcatca
    10681 cctgtccaag caatatctgc ataattcctt gtgaataagg agctgcaaga taacaaataa
    10741 tcggcactaa tgccattaac agggcttttg ccgaacgatt tcggtcgtgt aatcgtttaa
    10801 taataatagc agacaggcta aatcctgtaa ttataagcgg tagcacactc aataaagaca
    10861 attcagccaa atttatccac atagcaacca taaacaggaa aagaaagtgg attccaaatc
    10921 caatccaaaa acctcgtcta tttagcctac cgttaaaact aaaaagtgct ttataccaac
    10981 tcattttcta ccctaaagtt acatcaagta ccatcattac tacaaagcct aacatcaggc
    11041 ttaaagtcgc aatatcagta tttcccgaac tttgcgattc cggaatcagt tcttctacga
    11101 ccacaaaaat catcgctccg gcagcaaagg caagagcata aggtaaaata gccgtcatcg
    11161 acagcacaaa aaccgcccca atcaccgccg caataggctc aaccactgct gacatagagc
    11221 cataccaaaa tgctttcttc cgactatgcc cttcgcctcg aattggcaat gcaagtgacg
    11281 agccttcggg aatgttttgt aaccctattc cgattgccaa accgatagct cccataatgg
    11341 aagcatctac gcctgaaact tgggttgcca aagcaccgaa agccacccct atggctaatc
    11401 cttctggaat attatgaatg gtaatcgcaa gaaaaagcaa agtagatttt gataagcctt
    11461 gtttaagttg atcatttcct tcagcttgct caatcggctt actcaagtgc aaatgcggca
    11521 caacatagtc aattaatcga ataaaagctc caccggcaag aaagccaatc gctgcaggca
    11581 gccaagctaa agagccataa tccgcctcgg catattctaa agaaggtgca agcaaagacc
    11641 aaaacgaggc tgcaatcatt acacccgcgg caaagcccat catactgtct aataattttc
    11701 gattaaccga tttaaaaaaa tagacaaatg acgaaccgaa aatcgtacaa ccccaagtaa
    11761 ataaacccgc taaaaaagct tgccatattg gagaaagtga aaggaaatat tcaaacaaga
    11821 aatagtacca gctaaaataa atattggctc attctatcaa agattcaggg ttattaatat
    11881 agtgtagcca aaggctcaac gaagatttca cttgtaaaac ttagcaagaa aataaccgct
    11941 tgcatttccc tttcataaat acaat
//
LOCUS       DANJWC010000066        11917 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.066, whole genome shotgun sequence.
ACCESSION   DANJWC010000066 DANJWC010000000
VERSION     DANJWC010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11917
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.066"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..685
                     /gene="rpsB"
                     /locus_tag="PWX02_001308"
     CDS             <1..685
                     /gene="rpsB"
                     /locus_tag="PWX02_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005645552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDL1729323.1"
                     /translation="GVHYGHQTRYWNPKMKPFIFGARNGVHVINLEKTLPLFNEALAE
                     LTRIASNNGKVLFVGTKRAASEAVKLAAVDSQQFYVNHRWLGGMLTNWKTVRQSIKRL
                     KDLETQTQDGTFDKITKKEALMRTRELEKLELSLGGIKDMNGLPDAIFVIGADHEHIA
                     IKEANNLGIPVFAIVDTNSTPDGVNYIIPGNDDATRAIQLYLDSAVAAIKEGRGVETV
                     EVAEEASAE"
     gene            781..1632
                     /gene="tsf"
                     /locus_tag="PWX02_001309"
     CDS             781..1632
                     /gene="tsf"
                     /locus_tag="PWX02_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDL1729324.1"
                     /translation="MAEITASLVKELRERTGAGMMECKKALVEANGDIELAIDNMRKS
                     GQAKAAKKAGRVAAEGVILARVASGFGVLVEMNCETDFVAKDAGFLELANEVADFAVA
                     NKGTTIEALQAQFEEKRAALVAKIGENMNIRRVQYLEGEVIAQYLHGAKIGVLVAGAG
                     SEDELKKVAMHVAASKPEFVNPEDVSADVVAKEREIQIEIAMNSGKPKEIAEKMVEGR
                     MAKFTGEVSLTGQPFVMDPSQSVGQYLKSVNTSVSNFIRLEVGEGIEKVEEDFAAEVA
                     KITGGNA"
     gene            complement(1697..3259)
                     /locus_tag="PWX02_001310"
     CDS             complement(1697..3259)
                     /locus_tag="PWX02_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDL1729325.1"
                     /translation="MKSKQLFGILVAISLATAASCLMLIGSGFFPNPSIFEVILGVFL
                     IIALASSKWTYYLLLLPISIAYAIYTPVGIKFGKPTYEYIASIFATDMMESKEFIAQL
                     PILNIAAGIAIVLAVIFYRKITNRYQIRFLRNKTFVISAFVLMLFSLAPFKFFQEFYN
                     SSLKVKKELEVLNNLTLDSQWGQSTLSSTSNYDDYVLIMGESARKDYHHAYGYPAANT
                     PFMSSAKGTLIDGLTSGGTNTIASLRLMLTKPDTEKWEANYGLTLIDLIKSTGIKTYW
                     ISNQGYLGNYDTPISSIGNKSDVKIFTKAGDSLNTNVSDFELLPHFAKVIEEPAQGKR
                     FIFLHLYGSHPITCDRLTDYPKMFDDDKLPKRYFNVNCYVSSIKKTDELLKRVYDLLV
                     KNQENHQRSFSMVYLSDHGLSHEVSKENIVIHNGKDRSKLHYDIPLFKISSDDTQRNE
                     YKAFKSGLNFTNSIANWIGIENPLLDKEINLFNEQNDKSDYGLKKIIETQSTVEDPAV
                     PIPESYLYKATN"
     gene            3351..4067
                     /gene="pyrH"
                     /locus_tag="PWX02_001311"
     CDS             3351..4067
                     /gene="pyrH"
                     /locus_tag="PWX02_001311"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDL1729326.1"
                     /translation="MSKPIYKRILLKLSGEALQGSEGFGIDPSILDRMALEIKELLAE
                     GVEVGVVLGGGNLFRGAKLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAEVNAKLM
                     SAFQLNGICDTYNWSEAIKMLREKRVVIFSAGTGSPFFTTDSAACLRGIEIKADVVLK
                     ATKVDGVYDKDPAKYADAKLYNKLTYAQVIEQELQVMDLAAFTLARDHGMPIRVFNMG
                     KPGALRNVVFGTEEGTTISE"
     gene            4119..4676
                     /gene="frr"
                     /locus_tag="PWX02_001312"
     CDS             4119..4676
                     /gene="frr"
                     /locus_tag="PWX02_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDL1729327.1"
                     /translation="MINEIKKDTQDRMEKSLEALKGHIAKIRTGRAQPSLLDGIQVEY
                     YGSATPLRQVANVVAEDARTLAVNVFDKSLISAVEKAILTSDLGLNPSSAGTTIRVPL
                     PPLTEERRRDLIKIVKAEGEQGKVAIRNVRRDANDQIKALLKDKEISENEQHKAEEDI
                     QKITDTFIKKVDEVLAAKEKELLDF"
     gene            complement(4813..5496)
                     /locus_tag="PWX02_001313"
     CDS             complement(4813..5496)
                     /locus_tag="PWX02_001313"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL1729328.1"
                     /translation="MQLVFIRHGFSEWNAKNLFTGWRDVNLTERGIEEAKSAGQKLKA
                     AGFEFDIAFTSVLTRAIKTCNIVLEESDQLWIPQVKNWRLNERHYGALQGLDKKETAE
                     KYGDEQVHIWRRSYDISPPDLDPQDPNSAHNDRRYANLPKDLIPNAENLKITLERVLP
                     FWEDHIAPALISGKRVLVTAHGNSLRALAKHIEGIFDADIMDLEIPTGQPLVYELDDN
                     LKVISKRYL"
     gene            5664..6827
                     /locus_tag="PWX02_001314"
     CDS             5664..6827
                     /locus_tag="PWX02_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL1729329.1"
                     /translation="MLVKRINSYCWTAIFSGVISFSTPFSFANNLNQIQQKIQQQTSK
                     INEQKQKRNALQSTLKNQEVEMGKVLDKLQKTEMSLTEVRQTIKATETEIARLEKLEK
                     QQKEKLKEQLDSAYRSGVNPSVLERLMSEEAKNAERMTAYYQHMNNIRIDAIVEIRKT
                     QAALKARRDELKGQQKDHQTQLNEQKKQEKDLKKVQSQRESTLRLIDKTLEQDQSRLE
                     NLRSNEQALKQQLEQAQREAQQAEKREQQREKQAKSGQSKTTENVERTPIKAGRYTMP
                     VSGNIITPFGNNWHGVVIGASAGTSVRAMASGRVIMAQWLAGYGNMVAIDHGNGDISL
                     YGYNQSISVSKGSRVQGGQVIAKVGNSGGQSRSALYFGVTRKGKPINPLNLVK"
     gene            6828..7673
                     /locus_tag="PWX02_001315"
                     /pseudo
     CDS             6828..7673
                     /locus_tag="PWX02_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248088.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
     gene            7685..9520
                     /locus_tag="PWX02_001316"
     CDS             7685..9520
                     /locus_tag="PWX02_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HDL1729330.1"
                     /translation="MNTKLHFLTFCCLVAFSSMAQANQEQRLSDVDRERVENLQEKLA
                     EDSDDEPEIEPLVTLEVKGIQDKDAKKNVELYLDQIANEYADGSERYQYLVQTTVDKA
                     LRALGYYNSRYQFVQLPQEGKKPLLVLNVELDKETVKIDETDIVLQGDAKTDEDFAHL
                     VSKAPKQGSRLNHKTYDNFKGDIESTAFSKGYFDGHWLYHRLEVYPTDHKADWRLGYD
                     SGKRYHYGQIRFVDSQIKEEYLQNILKIHSGEPYLANDLSILTQDYLSSNWFSSVMVE
                     PHLNEQDKLVDLNVLFQPKKRNEVEIGIGYETEVGPRLQLNWKKPWLNSRGHSIESRT
                     YVSKPEQTFEFGYNIPLREDPLHYYYQLSGTLENEDQNDTKSTAATLGLQRFWTHETG
                     WSFSVGLKARYDSFTQANDRFKTLLVYPTASLNRTRTDGKPYPLWGDTQKLTVNWGSR
                     AVGSDVNFYSYKASTSWARTYFDNHRFYLRAELGYLKANQFERIPPALRYFAGGDMSV
                     RGFGYKDISPRDPSNGKRVGGSHLATGTVEYQYQVYPNWWGAIFYDAGLASNKFKTQD
                     LYYGTGLGVRWASPIGAIKFDVATPVKSPDNSKGIQIYIGLGSDL"
     gene            9601..>11917
                     /locus_tag="PWX02_001317"
     CDS             9601..>11917
                     /locus_tag="PWX02_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HDL1729331.1"
                     /translation="MTDENKQTATNQTDSAQEPQATQPKKSKWRFLKHGFCLTFILLL
                     LAILFLCTGYGQRKVLSWVDKFVEPLQLGQVQGSLQDGLQLSDARFVMDGVNVTVGQA
                     DLHIGFSCLLHYEMCLNNLSLKDTEVLIDTTKLPPSQPKEETEPFTELNLPLGISAQN
                     INLENIKVKVDDMDIHLNHFHTALSGKGRAITVHPTQLEGLNLLLALKLDETDTEQAV
                     DFAQKNAKTEALSATNPQEIADKVIQEAVVEKTSEKVVEGFHQLQRKTVAQAEQVDNK
                     VDWAAIKAQLAQPLLDKNIRFSLPLDLNIEQIDIANVAIAQKAKDEKGNLIEPISLLN
                     VASLQLQAQAKDQHINLKSLDFKSDQGDLTASGSLSLQDNYPLSWTLSGHEAKTAKVK
                     LPFNQIEASLSGELYDKTSLKVKTAGAISASLEGNVALAQAKTPFDLSLKSESMRYPF
                     APQQNGDVLSLENIAMRLNGNLLDYALEAKVSAKGMGVPASSASLTGKGELTHFNIQD
                     LSLNTLEGTAKIDGLVDWSEGVAWRSNLKLQHINTKSLLADWPAVLSGSLSSEGYAGR
                     GNSASGWKADVSNIDIKGSLLQKNLHLSGNLKADHQQLLDVPGLSLVYGENKIDLKGV
                     LGEKSDFYADIKAPNLQGLVPNLKASVNGNAKLSGKVSEPNIDLDLVASNVGYEQFKL
                     QNLTAKGKITTEKTIQGDLTLGLRQFAYNEIKVENATLLLKGNEENHSLKLSAKGNPV
                     GADLQISGKFDRLKEIWEGQLSQVSIQSTEFG"
BASE COUNT         3831 a         2116 c         2591 g         3379 t
ORIGIN      
        1 aggcgttcac tatggtcacc aaactcgtta ttggaaccca aaaatgaaac cattcatttt
       61 cggtgctcgt aatggtgttc acgtgattaa cttagaaaaa acattacctt tatttaacga
      121 agcattagcg gaattaactc gtattgcctc taacaacggt aaagttttat tcgtaggtac
      181 aaaacgtgca gcgtcagaag cagttaagct agctgctgta gatagccaac aattctacgt
      241 taatcaccgt tggttaggtg gtatgttgac taactggaaa acagttcgtc aatcaatcaa
      301 gcgtttaaaa gatttagaaa ctcaaactca agacggtact tttgacaaaa tcactaaaaa
      361 agaagcgtta atgcgtacgc gtgagttaga gaaattagag ttaagcttag gcggcattaa
      421 agatatgaac ggtttaccag atgcaatctt tgttatcggt gctgatcacg aacatatcgc
      481 aattaaagaa gcaaacaact taggtattcc ggtatttgcg attgttgata ctaactcaac
      541 tccagatggc gttaactaca tcattcctgg taacgatgat gcaacacgtg ctatccaact
      601 ttacttagat tcagcagttg ctgcaatcaa agaaggtcgc ggtgttgaaa ctgttgaagt
      661 agcagaagaa gcttctgcgg aataattcag ttgagggggc aggttttaac ctgccctaaa
      721 caatgaaatt tctaggcagg tgagagcctg ttttaccata tcaaataagg ggaatttaaa
      781 atggctgaaa tcacagcatc attagttaaa gaacttcgtg aacgtaccgg cgcaggtatg
      841 atggaatgta aaaaagcatt agttgaagcg aacggtgata ttgaattagc aatcgataat
      901 atgcgtaaat ctggtcaagc gaaagcggct aaaaaagcag gtcgtgttgc agctgaaggt
      961 gtaatccttg ctcgtgtcgc ttcaggtttt ggcgtattag ttgaaatgaa ctgtgaaact
     1021 gacttcgttg caaaagatgc cggtttctta gaattagcga acgaagttgc tgattttgca
     1081 gttgcaaata aaggtacaac tatcgaagca ttacaagcac aatttgaaga aaaacgtgcg
     1141 gcattagttg cgaaaatcgg tgagaatatg aacattcgcc gtgttcaata cttagaaggc
     1201 gaagttattg ctcaatactt acacggtgcg aaaattggtg tattagtagc aggtgcaggc
     1261 tctgaagatg agcttaagaa agtagcaatg cacgttgctg caagcaaacc tgaatttgta
     1321 aacccagaag atgtttctgc tgatgtagta gcaaaagaac gtgaaattca aattgaaatt
     1381 gcgatgaatt ctggtaaacc aaaagaaatc gcagaaaaaa tggttgaagg ccgtatggct
     1441 aaattcaccg gtgaagtttc attaaccggt cagccgtttg taatggatcc atctcaatct
     1501 gtaggtcaat atttaaaatc agtaaatact tcagtttcta actttattcg tttagaagta
     1561 ggtgaaggta ttgagaaagt agaagaagat ttcgcagcag aagtagcgaa aatcactggc
     1621 ggtaacgctt aatttttctg atactaaaaa ctaaaaaagc aggcttaaag cctgcttttt
     1681 ttgttactta cttttgctaa ttagtcgctt tatataagta gctttcagga ataggaacag
     1741 ctggatcttc tacagtagac tgcgtttcaa tgattttctt tagcccataa tcacttttat
     1801 cgttttgttc gttaaaaaga ttaatttctt tatctaataa cggattttcg ataccaatcc
     1861 aattcgcaat actattagtg aagtttaagc cggatttaaa ggctttatac tcattacgtt
     1921 gggtatcatc gcttgaaatt ttaaacagcg gaatatcgta gtgtaattta cttctatcct
     1981 ttccattgtg gataacaatg ttctcttttg aaacctcgtg cgataaaccg tgatcagaca
     2041 aataaaccat tgagaatgaa cgctggtgat tttcttggtt tttcactagc aaatcatata
     2101 cccgtttcag caattcatcg gttttcttga tagatgacac gtagcaattt acattaaagt
     2161 agcgttttgg cagtttgtca tcgtcaaaca ttttcgggta atcggtgaga cgatcgcagg
     2221 taataggatg cgagccatat aaatgcagaa agatgaaacg cttcccttga gcaggttctt
     2281 caatcacttt agcaaaatgc ggtaacaatt caaaatcgct cacattggta tttaatgaat
     2341 cgccggcttt agtgaaaatt ttgacatcac ttttattacc gatagaggaa atcggcgtat
     2401 cgtaattgcc caaatagcct tgatttgaga tccaataggt cttgataccg gtagatttga
     2461 ttaaatcaat taaggttaac ccataatttg cttcccattt ttccgtatcg ggcttagtga
     2521 gcattaagcg taatgaggcg atagtgtttg tgccaccgga ggttaaaccg tcaatcaagg
     2581 ttccttttgc cgaactcata aaaggcgtat tggcagccgg atagccatag gcgtggtgat
     2641 aatcctttct cgcactttcg cccataatca gtacataatc atcgtagttt gaagttgagc
     2701 tgagggttga ttgtccccat tgtgaatcta gcgttaaatt gttcagcact tccaattctt
     2761 tctttacctt taggctcgaa ttgtagaact cctgaaaaaa tttaaacggt gcaagggaaa
     2821 acagcattaa tacaaaagcc gaaatcacga atgttttatt gcgtaaaaaa cggatttgat
     2881 agcgattggt aatttttcga taaaaaatga ccgctagtac aatcgcaatg cccgccgcaa
     2941 tatttaaaat cggcagttga gcaataaact ctttgctttc catcatatcg gtagcaaaaa
     3001 tagaggcgat atactcgtaa gtcggtttac cgaattttat cccaaccggg gtataaattg
     3061 cataagcgat gctgattggc agtaagagta aataataagt ccatttagag ctggcaagag
     3121 cgatgatgag gaatacgccc aaaatgactt caaagatact cggatttgga aaaaagcctg
     3181 atccgattag cattaaacag ctagcagcag ttgccagcga aatagcgaca agaatgccga
     3241 aaagttgttt ggatttcatt gtagtgaata tttttagttt atcgttatat tggattgttc
     3301 tataataacg taattttgtg tttagtccat acagttaaga ggcaattaaa atgagcaaac
     3361 ctatttataa gcgaattctt ctcaaattaa gtggtgaagc cctacaaggc agtgaaggtt
     3421 ttggtatcga cccgtctatt ttggatcgta tggcgttaga gattaaagaa ttacttgctg
     3481 aaggcgttga ggttggtgtg gtacttggcg gcggtaactt attccgtggt gcaaaattag
     3541 ccaaagcagg aatgaaccgt gtagtgggcg accatatggg tatgcttgcc accgtaatga
     3601 acggtttggc tatgcgtgat gccttacacc gtgcagaggt aaatgctaaa ttaatgtctg
     3661 cgttccaatt aaatgggatt tgcgacactt acaactggtc tgaagcaatt aaaatgttgc
     3721 gtgaaaaacg tgtggtgatt ttctctgccg gtacaggtag cccgttcttt accaccgatt
     3781 cagctgcttg cttgcgtggt attgaaatta aagctgatgt ggtattaaaa gcaactaaag
     3841 ttgatggcgt ttatgataaa gacccagcaa aatacgcaga tgcaaaacta tataataaat
     3901 taacgtatgc gcaagttatt gagcaagaat tacaagtaat ggacttagca gcattcacgc
     3961 ttgctcgtga ccacggaatg ccaattcgtg tctttaatat gggcaaaccg ggggcgttac
     4021 gcaatgtggt attcggtact gaagaaggta caaccatttc tgaataatta attttctctg
     4081 tttaactctc agttactaaa taaaaggata aaaaatcaat gattaacgaa atcaagaaag
     4141 atacccaaga tcgtatggaa aaaagccttg aggctttaaa agggcatatt gcgaaaattc
     4201 gtacaggtcg tgcccaacct tctttattag acggtattca agtggaatat tacggctctg
     4261 cgactccatt acgtcaagta gcgaacgtgg tagctgaaga tgcccgtact ttagcggtaa
     4321 acgtatttga taaatcttta atctctgcgg tagaaaaagc aattttaacc tcagatttag
     4381 gtttaaaccc atcttctgcc ggcacaacca ttcgtgttcc gcttccacca ttaacggaag
     4441 aacgtcgtcg tgatttaatc aaaatcgtta aagcagaagg tgagcaaggc aaagtggcaa
     4501 ttcgtaatgt tcgtcgtgat gctaatgacc aaatcaaagc gttattaaaa gataaagaaa
     4561 tcagcgaaaa cgaacaacac aaagctgaag aagatattca aaaaatcacc gataccttca
     4621 ttaaaaaagt tgatgaagta ttagcagcaa aagagaaaga gttgttagat ttctaatcta
     4681 ctttaaaaca ataaggcgaa tgtatgattc gcctttattt ttgttctttg ccatttgcaa
     4741 aaaattaaca aaaaataacc gcttgtaatg tgttgcacta caagcggtta ttttttatcg
     4801 gaaatttacc aattataagt aacgtttgct gattactttt aagttgtcat ctagttcata
     4861 cactaatggc tgaccggttg ggatttctaa gtccataatg tctgcatcaa aaatgccttc
     4921 gatgtgtttt gctaatgcac gtaatgagtt accgtgagca gtaactaata cgcgtttacc
     4981 tgaaattaat gcaggtgcaa tatgatcttc ccagaatggt aacacacgct caagggtgat
     5041 tttgaggttt tctgcatttg gaattaaatc ttttggtaag ttagcataac ggcggtcgtt
     5101 atgtgctgag tttggatcct gtgggtctaa atccggtgga gagatgtcat aagaacgacg
     5161 ccagatgtga acctgttcat caccatattt ttcagcagtt tcttttttgt ctaaaccttg
     5221 taatgcaccg tagtgacgct cgtttaaacg ccagtttttc acttgaggga tccataattg
     5281 gtctgattct tctaatacga tgttacaagt tttgatcgca cgggttaaaa ccgaagtaaa
     5341 cgcaatatca aattcaaaac cggctgcttt taatttttga ccggcagatt ttgcttcttc
     5401 aatcccacgt tctgttaaat taacatcacg ccaaccggta aataagtttt tagcattcca
     5461 ttcactgaaa ccgtgacgga taaatactaa ttgcataatg actccttagc tttgagtaaa
     5521 attaatggat ttgaaattgt gctatttata gcaaaattct ttatattttg aaatagatag
     5581 ttaaattctt tcactaaaaa gctgatctta agcaaataac cgattagaat ataaccttaa
     5641 taatattttg aagaaataaa ggtatgctgg tgaaacgaat aaattcttac tgttggactg
     5701 ctattttcag tggggtaata agcttttcta cccctttttc ttttgcaaat aatcttaatc
     5761 agattcagca aaaaatccaa cagcaaacct caaaaattaa tgaacaaaag caaaaacgta
     5821 atgcgttgca atctacccta aaaaaccaag aagtagaaat ggggaaggtg ttggataaat
     5881 tacaaaaaac cgaaatgtct ttaacggaag ttcgccagac cattaaagcg actgagactg
     5941 aaattgcccg tttagaaaag ttggaaaaac agcagaaaga aaaattaaaa gagcagttgg
     6001 actctgccta ccgttctggg gtgaaccctt cggtcttaga gcgtttaatg tctgaagagg
     6061 caaaaaatgc ggaaagaatg accgcttatt atcagcatat gaataatatt cgcattgatg
     6121 cgattgttga aattcgtaaa acccaagctg ctcttaaagc cagacgtgat gaattgaaag
     6181 gccagcaaaa agatcatcaa acgcaactta atgaacaaaa gaaacaagag aaagatttaa
     6241 agaaagtaca aagccagcgt gaaagtacct tacgtttaat cgataaaacg cttgagcagg
     6301 atcaaagccg attagagaat ttacgcagca atgaacaggc actaaaacaa caattagaac
     6361 aagcgcaaag agaggctcag caagcagaaa aacgtgaaca acagcgggaa aaacaggcaa
     6421 aatccggtca gtccaaaaca actgagaatg ttgagagaac accgatcaag gctggtcgat
     6481 atacgatgcc ggtgtctggc aatattatca ctccttttgg taataattgg cacggtgtag
     6541 tgattggtgc ttctgccggt acttcggtta gagcaatggc gagcgggcga gtcataatgg
     6601 ctcaatggct tgcagggtat ggcaatatgg ttgcgattga ccacggcaat ggtgatattt
     6661 ccctttatgg ctataaccaa tctatttcag tcagcaaggg tagccgagtg caaggcggac
     6721 aggttattgc gaaggttggt aacagtgggg gacaaagccg ttctgcactc tattttggtg
     6781 ttacccgtaa aggcaagccg attaatcctc taaatttggt gaaatagatg tttaaacaaa
     6841 ttgacaagcg gttgaatttt aacatttttt tgcaaatgtt tgtgcttttt acaccgcttg
     6901 tacaagcagc tcaaatggcg attgtaattg atgatgtggg ctatcgaata aaagaagatc
     6961 gagaaatctt agccttaccc aaggcggtaa gcgtggcgat tattcctgtt gcaccctatg
     7021 caacagaacg ggcaaaggac gcttataacc aaaaacggga tattttgatt catttgccaa
     7081 tggaacctaa atcgaaacag ccaattgaag agggggggta ttcatattgg tgataacgaa
     7141 gagaaaattc ggaaacttat tcatacttct cgtggtcaag taccatatgc tattggttta
     7201 aataaccata tgggcagtgg cgcaacttca gactctgcta caatgcaaca tttgatgaaa
     7261 gtgttaaaag aaaatacgct tttcttttta gacagtaaaa ctattggcag tagtgtggcg
     7321 gctaaaacgg ctcgtcaatt tggtattaat acgctggaga gagacatttt tttagatgat
     7381 agcgattgct tgctgatgta caaaaacagt ttgcacatgc aattaatcac gcacgaaaaa
     7441 acggggtaga ggtggtgata ggacaccctc gcaagaatac gattagtgta ctgaagcaaa
     7501 atttagcaca actacctcaa gatattgaat tggtgagtgt tggaaacttg tggcgtaatg
     7561 aaaaaacagt gcctgacaaa ccattcatta tgctctttga gcaagagcca gcgttaagtt
     7621 ctgttccgcc ttatgacagt attccgcttt tgcgtggcat tccaaaagaa taaaaaggtt
     7681 actaatgaat acaaaactac attttttaac attctgctgt ttagtggctt tttcttcaat
     7741 ggcacaagca aatcaagagc aaaggctgtc tgacgtagat cgtgagcgag ttgaaaatct
     7801 gcaagaaaaa ttagcggaag atagtgatga tgagccagaa atagagccat tagtcacttt
     7861 agaagtcaaa ggcattcagg ataaagatgc caagaaaaat gtggagctat atttagatca
     7921 aattgccaat gaatatgcag atggttcaga gcgttatcaa tatttggtgc aaaccacagt
     7981 agataaagct ctgagagcct tgggctatta taatagccga taccaatttg tgcagctacc
     8041 tcaggaggga aagaaaccgc tcttagtatt aaatgttgag ctagataaag agacggtaaa
     8101 aattgatgaa accgatattg ttttgcaagg cgatgcaaaa acggacgaag atttcgctca
     8161 tctagtcagt aaagccccaa aacaaggtag tcgtttaaac cataaaacct atgataattt
     8221 taaaggtgat attgaaagta ccgcttttag taaagggtat tttgacggtc attggcttta
     8281 tcaccgtttg gaagtttatc cgaccgatca taaagcggat tggcgtttag gctatgatag
     8341 cggaaaacga taccattatg gacaaatccg gtttgttgat agccaaatca aagaagaata
     8401 tttgcaaaat attcttaaaa ttcattccgg tgagccatat ttagctaacg atctttcgat
     8461 tttaacccaa gattaccttt ccagtaattg gttttcatca gtgatggttg agccacattt
     8521 aaatgaacag gataaattag tggatttaaa tgtgttattt cagcctaaaa aacgcaatga
     8581 agtggaaatt gggataggct acgaaactga agttggccca aggttacagc ttaactggaa
     8641 gaaaccttgg ttaaatagcc gaggtcatag tattgaaagc cgaacgtacg tttctaagcc
     8701 ggagcagaca tttgaatttg gttataatat tccacttaga gaagatccgc tacattatta
     8761 ttaccaatta tccggcactt tagaaaacga agatcaaaac gatacaaaat caaccgctgc
     8821 cacccttggc ttacaacgtt tctggactca cgaaaccggt tggtcatttt ctgttggttt
     8881 aaaagcccgt tacgattctt ttactcaagc taatgatcga tttaaaacct tattagttta
     8941 cccaacggca tcattgaatc gaaccagaac agatggcaag ccttacccat tatgggggga
     9001 tacacaaaaa ttaacggtaa actgggggag tcgtgcagta ggttcagatg taaactttta
     9061 tagttataaa gcctcgacat cttgggcgag aacttacttt gataaccatc ggttctattt
     9121 aagggcggag ttggggtatt tgaaagccaa tcaatttgaa cgtattccac cggcgttacg
     9181 ctattttgcc ggtggcgata tgagtgtgcg gggctttggc tataaggata tttcccctcg
     9241 tgatccgagt aatggcaaac gtgtcggagg ctctcattta gccacaggca cagtagaata
     9301 tcagtatcag gtttacccta attggtgggg tgcaattttt tatgatgcag gcttggcatc
     9361 gaataaattc aaaactcagg atctctacta cggtacgggc ttaggggtgc gttgggcatc
     9421 acctatcggg gcgattaaat ttgatgtcgc cacgccggta aaatcaccag ataattccaa
     9481 aggaatacaa atttatattg gtttaggttc cgatctgtaa atgaaaacaa gcggtcgaat
     9541 ttcagtaaaa tttcgcatac cgcttgttta ctttttatca cctcataaat atgcacgatt
     9601 atgactgacg aaaataaaca aacagccaca aatcaaacgg actcagcaca agagccacaa
     9661 gcaacacagc ctaaaaaatc aaagtggcgg tttttgaagc acggcttttg tctgacgttt
     9721 attctacttt tactcgccat tctctttcta tgcacaggtt acgggcaacg taaagtgtta
     9781 agctgggtag ataaattcgt agagccatta caacttgggc aagtgcaagg tagcctgcaa
     9841 gatgggttac agctttctga tgcccgattt gtgatggacg gtgtgaatgt aacggttggg
     9901 caagcagatc tgcatattgg ttttagctgc ttgctccact atgaaatgtg tttgaataat
     9961 ctcagcctta aagatacaga ggtgttgatt gatacgacta aattaccccc ttctcagcca
    10021 aaagaagaga ccgaaccttt caccgaatta aatttgccat tggggatttc agctcaaaat
    10081 atcaatttag agaatatcaa ggtcaaagtt gatgatatgg atattcatct taatcatttc
    10141 cacactgcac tttctggtaa agggcgggct attaccgttc acccgacaca gttagagggg
    10201 ctaaatctgc ttttagcctt aaagttagac gaaactgata ctgaacaagc ggtcgatttt
    10261 gcacaaaaaa atgcaaaaac agaggcgtta tcagcgacaa atccacaaga gattgctgat
    10321 aaagtgattc aagaggcggt ggtggaaaaa accagtgaaa aagtcgtaga gggctttcac
    10381 caattacaga gaaaaactgt tgcacaggcc gaacaggtgg ataataaagt ggattgggca
    10441 gcgattaaag cacaattagc acagccgtta ttggataaaa atattcgctt tagtttgcct
    10501 cttgatctca atattgaaca gattgatatt gcgaatgtag cgattgctca aaaagctaaa
    10561 gatgaaaaag ggaatttgat tgagccgatc tcattgttaa atgttgcctc tttacagctt
    10621 caggctcaag caaaagatca gcatatcaac ctaaaaagtt tagatttcaa aagcgaccaa
    10681 ggggatctaa ccgcaagcgg ttctttatcg ttacaagata attatccgct aagttggacg
    10741 ctaagtggtc acgaggcaaa aacggcgaaa gtaaaattgc cgttcaatca aattgaagcc
    10801 tctctttccg gtgagctata tgacaaaacc agcctaaaag tgaagacggc tggtgcaatt
    10861 agcgcctcgt tggagggcaa tgttgcactg gctcaggcga aaactccctt tgatttatcg
    10921 ctgaagagtg agtcaatgcg ttatccgttt gctcctcagc aaaatggtga tgtgctgagt
    10981 ttagagaata tcgccatgag gcttaatggc aatctcctgg attacgcttt ggaggctaaa
    11041 gtcagtgcca aaggaatggg cgtgcctgca agctcggcta gtttaactgg caaaggggaa
    11101 ttaacccatt ttaatataca agatttaagt ctcaatacgc tggaaggtac ggcgaagatc
    11161 gacggtttag tggattggag cgagggcgtt gcgtggcgtt caaatttgaa acttcagcat
    11221 attaacacta aatccctact ggcagactgg ccggcggtat tgtccggttc tttatcttcc
    11281 gaaggttatg caggacgggg caattcagct agcggatgga aagcggacgt ctcgaatatt
    11341 gatattaaag gttcgttatt acagaaaaat ctacacttaa gcggtaattt aaaagccgat
    11401 catcaacaac ttttagatgt tccgggctta tcgctggtgt atggtgaaaa taagatcgat
    11461 ttaaaaggcg tgttaggcga aaaatctgat ttctatgcgg atattaaagc ccctaatttg
    11521 caagggttag tgccgaattt aaaagccagc gtgaatggta atgccaaatt atcgggtaag
    11581 gtttcagaac ctaacattga tttagattta gtggcctcaa atgtaggcta tgagcaattt
    11641 aaactgcaaa atttaaccgc aaaaggcaaa attaccactg agaaaaccat tcaaggggat
    11701 ttaaccttgg ggttacgtca atttgcctat aatgaaatca aagtggaaaa tgccacttta
    11761 ttgctaaaag gaaatgaaga aaatcatagc ttgaaattaa gtgcaaaagg caatccggtt
    11821 ggtgctgatt tgcaaatttc tggaaaattt gaccgtttga aagaaatttg ggaagggcaa
    11881 ttaagccaag tttcgattca atccaccgag tttggtc
//
LOCUS       DANJWC010000067        11910 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.067, whole genome shotgun sequence.
ACCESSION   DANJWC010000067 DANJWC010000000
VERSION     DANJWC010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11910
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.067"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..246
                     /locus_tag="PWX02_001318"
     CDS             <1..246
                     /locus_tag="PWX02_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL1729332.1"
                     /translation="LVLEERFKGYMAFGIAIWVFLQGFVNLGVASGLLPTKGLTFPLV
                     SYGGSSLVIMSIAIAVLLRIDHENRAERIGHAKIKTI"
     gene            365..1420
                     /gene="murG"
                     /locus_tag="PWX02_001319"
     CDS             365..1420
                     /gene="murG"
                     /locus_tag="PWX02_001319"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDL1729333.1"
                     /translation="MAKKLLVMAGGTGGHVFPAIAVARELQKQGWQIRWLGTKDRMEA
                     TLVPKHGIEIDFIEISGLRGKGVAALLKAPFAIFKAVMQARKIIKNYQPDAVLGMGGY
                     VSGPGGIAAKLCGVPVILHEQNAVAGLTNVWLSKIARKVLQAFPTAFKEAEVVGNPVR
                     EDLSALPLPNERFAERGYPINILVMGGSQGARVINQTVPEVAKVLGNNVFISHQVGKG
                     NLAGIEEVYQQTGNGIASEFIDDMKAAYEWADLVICRSGALTVCEIAAVGLPAVFVPF
                     QHKDRQQFLNANYLAADGAAVIIEQSDFTPKALQQALEPLIANRQLLLDMAMRARAKS
                     TPMAAKRVAEVIIQEAN"
     gene            1496..2923
                     /gene="murC"
                     /locus_tag="PWX02_001320"
     CDS             1496..2923
                     /gene="murC"
                     /locus_tag="PWX02_001320"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013527829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDL1729334.1"
                     /translation="MKNFQDKVKKLVPEMRRVSQIHFIGIGGAGMSGIAEVLLNEGYQ
                     ISGSDIADRAVTQRLSAAGAKVFIGHQAENIAGASVVVVSTAIDETNPEIIAAREARI
                     PVIRRAEMLAEIMRFRHGIAIAGTHGKTTTTAMISMIYTEAKLDPTFVNGGLVKSAGK
                     NAHLGASRYLIAEADESDASFLHLQPMVSVVTNIEPDHMDTYGGDFEKMKETYVRFLR
                     NLPFYGLAVMCADDETVMEIAPRVGRQVITYGFSENADYRIEDYQQTGFQEHYTVVCP
                     SGERIDVLLNVPGMHNALNATAALAVAKEEGIGNEAILAALADFQGAGRRFDQLGSFI
                     RPNGKIMLVDDYGHHPTEVDVTIQAARSGWEGKRVVMIFQPHRYSRTRDLFDEFVQVL
                     SKVDALILLDVYAAGEAPIVGADSKALARSIRNLGKVDPILVSDTDQLGEVLDQIIQD
                     GDLILAQGAGSVSRISRGLAESWKN"
     gene            3013..3924
                     /locus_tag="PWX02_001321"
     CDS             3013..3924
                     /locus_tag="PWX02_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDL1729335.1"
                     /translation="MSIKNEKIAVLFGGVSQEREVSLNSGAAVTAALKSLGYNVEGID
                     TKEFPIEKLKEKGIQRVFNILHGGIGENGVLQGALEQMGIPYTGCGVMASAVTLDKFR
                     TKLLWNAVGLPTADMVVVQRGQVVDFDQIIAKLGLPVFVKSSCEGSSVGVFKVKTKEE
                     LAPAIHEALKFDSIVLVEEFLAGNEYSVPVLDGQVLPAVQVIPDGEFYDYHAKYVSDN
                     TQYVVPALNDERQAEVAKLVKAAYDVVGCRGWSRIDVMEDGEGKFRLVEVNTNPGMTS
                     HSIFPKSAATVGISFEKLVERVLELSA"
     gene            3924..4715
                     /locus_tag="PWX02_001322"
     CDS             3924..4715
                     /locus_tag="PWX02_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDL1729336.1"
                     /translation="MGIFRKKNSTNVRLNVLNPKTPVSPRKWLVFIKPLIVLLCTLFI
                     FGVYSNWQNILESWDKTPIRAYALTHKTQFTTNADIRETLSKEPALKGYFGQNIKEVK
                     DKLLSIPWVKDVAVRKLYPDRLSITLLEHKPVAIWNDTNFVSEQGTVFSLPKDRIDKN
                     GLPLLYGPDTEGKSVLEAWNKIRADLNARNLDLTSVSVDNRGSWTIRLSNQVELRLGR
                     GEWTSKIDRFVTIFPEIHIPEGNRLAYVDLRYPHGAAVGFSPIKN"
     gene            4738..6009
                     /gene="ftsA"
                     /locus_tag="PWX02_001323"
     CDS             4738..6009
                     /gene="ftsA"
                     /locus_tag="PWX02_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDL1729337.1"
                     /translation="MTKAVESKIIVGLDIGTSKVVAVVGEVLPDGVINVMGAGVCPSK
                     GVDRGGVIDLDAAVNSIQRAIEQAESIADCQIMGVTLAISGQHIVGLNEAGSSTLSGG
                     IVTQDNIESAVDMAKAIKLQDGLETLHMIPQEYYVDRLPATKNPIGLSGMRLQVQAHL
                     IACHQAWLKNLKNAVEHAKLKVDQVVFSGLASSYSVLTEDEKELGVCLIDIGGGTMDV
                     LVYTDGALRYSKVIPFAGNNITDYLARVFTTSRPEAESLKVGYGSAISPPTHNSDKKI
                     EVAGLGGRMARIFTRAQVATVTSQCYNDLLKVVEEELTQLRHELFQKGIKQELIAGVV
                     ITGGGSQIEDIVECAKSIFGSQVRVGYPLNITGLTDYVNKPQYATALGLLQYSHYNYT
                     EQGTSTDSNEDIFAPIGRGTKKIIDFFKSKF"
     gene            6042..7289
                     /gene="ftsZ"
                     /locus_tag="PWX02_001324"
     CDS             6042..7289
                     /gene="ftsZ"
                     /locus_tag="PWX02_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDL1729338.1"
                     /translation="MFEPIIEQTQDAIIKVVGVGGGGGNAVDRMSRSADDIKGVEFFD
                     VNTDAQVLRKRTTRQTIQIGASTTKGLGAGADPMVGKQAAEEDREAIANALKGANMTF
                     IAVGMGGGTGTGAAPVVAQIAKEQGSLTVGVVTKPFRFEGPRRMRFADQGIKELSQYV
                     DSLIIIPNDKLRGLGKQTTAVDAFAAANDVLSNCVLGITNMITSSGGSTGADINVDFA
                     DVRTVMSGKGHAMIGTGFAEGEVGEGRAEKAMNDAISSPLLENVDISGASGMLINISA
                     GTDFLLEEVYAMMDLIYGFATEDAAIVFGCNYYPEMDGKVSVTLVATGIGQPEEALHM
                     PHKAQPVYAQQGNHVQATQPTQPNHAVQQPNFTQPSSYGQPNQFSGQPQPLQQPVAPK
                     PQSVDTAQIFNPNSIPGFMRNSQ"
     gene            7321..8241
                     /gene="lpxC"
                     /locus_tag="PWX02_001325"
     CDS             7321..8241
                     /gene="lpxC"
                     /locus_tag="PWX02_001325"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HDL1729339.1"
                     /translation="MTKQRTLKQAAKVTGIGLHSGKKVTLTLRPAPANTGVIYARTDL
                     EPVVYFPASADSIRDTQLCTCMVNDDGVRISTVEHLNAAMSALGLDNVIVEVDAPEIP
                     IMDGSSSPFIYLLLDAGIEEQDAPKKFIRIKETVRVEEGDKWAELKPYNKGFKLNFTI
                     DFEHPLIPKNVGSYSIDFSAENFIQQLSRARTFTFMKDVEYLQSIGLALGGSLDNAIV
                     LDDYRILNEDGLRFKDELVRHKMLDSIGDLYMCGYNILGEFSAYKSGHGVNNKLLRTV
                     LENKNAWEFVTFEDKAEVPQGYQVQQEIFI"
     gene            8414..10096
                     /gene="recN"
                     /locus_tag="PWX02_001326"
     CDS             8414..10096
                     /gene="recN"
                     /locus_tag="PWX02_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDL1729340.1"
                     /translation="MLTQLTINNFAIVRHLILELNEGMSVITGETGAGKSIAIDALGL
                     CLGYRSESGMIRNGSDKADISATFTMQPHSPAYLWLQEHELLDEDNPQECILRRMINI
                     DGRSKAFVNNRSLPVSQLRELGQYLIHLNGQHAPQLLLKNEYQLEIVDHYATLQPLLM
                     KMAKQYGNWKELNKQVKNFQKQCQENEARKQLLEYQVEELDEFAIKANEFEEMEEKYN
                     ILSSSEELTELSDEITRLLSDDEINANSLIYKALRNAENLAEVDSQYQGILDMLNEAL
                     IQIQEASSEVAQLASKIEQDPELLQELDNRIAQTISLARKHHIQPNELWQYHATLKAE
                     LQELLDFEDNEEQLIQNEKQAYQECITLAEEIYQKRLDAAHKLSEQVTKQIKHLAMEN
                     GEFFIHIEHNLEKMSANGADYVEFNLRSNLGQQAQPLAKIASGGELSRISLAVQVLTA
                     NKLSTPTIIFDEVDVGISGSAATTVGKLLRKLGEKCQVLCVTHLPQVASYGNQQFNVQ
                     KYVENNQTETQMTLLTEAERIHALARLLGGSEITETVLANAKEMLNLAKNNE"
     gene            complement(10143..10218)
                     /locus_tag="PWX02_001327"
     tRNA            complement(10143..10218)
                     /locus_tag="PWX02_001327"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10183..10185),aa:Ala,seq:ggc)
     gene            complement(10299..11738)
                     /gene="gltX"
                     /locus_tag="PWX02_001328"
     CDS             complement(10299..11738)
                     /gene="gltX"
                     /locus_tag="PWX02_001328"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDL1729341.1"
                     /translation="MKIETLFPLDPNVKVRTRFAPSPTGYLHVGGARTALYSWLYAKH
                     NDGEFVLRIEDTDLERSTPEATAAILEGMEWLDLAWEHGPYFQTKRFDRYNQVIDQML
                     EAGTAYRCYCSKERLDEVRAEQEANKEKAKYDQHCLHNPQNDPSQPHVVRFKNPTEGS
                     VVFDDAVRGRIEIANSELDDLIIRRTDGSPTYNFCVVVDDWDMGITHVVRGEDHINNT
                     PRQINILKALGAPIPTYAHVSMINGDDGQKLSKRHGAVSVMQYRDDGYLPEALINYLV
                     RLGWGHGDQEIFSRDEMIELFDIHSVSKSASAFNTEKLQWLNQHYIRSLAPSYVAKHL
                     EWHMQTQGIDFTSGPKLEEIIPVLGERCKTLKELAESSRYFYQEFKAYDEKAAAKNFK
                     AEAVAPLAKLLEKLTACNDWTVENIHEAMNQTAAELEIGMGKVGMPFRLAVTGSGQSP
                     SMDITAKLVGKERTLARLQKAIEFIQANS"
BASE COUNT         3647 a         2235 c         2679 g         3349 t
ORIGIN      
        1 ttggtgctgg aagagcgttt taaaggctat atggcatttg ggattgcgat ttgggttttc
       61 ctacaaggct tcgtaaactt aggggtggca tcaggtttgc tgccaacgaa gggcttaacc
      121 ttcccattag tgagttatgg tggttcgagt ttggtaatta tgtcgattgc gattgcagtt
      181 ttgctacgaa ttgaccacga aaaccgtgcc gagcgaatcg ggcacgcaaa aattaaaacc
      241 atctagccga gcggtctgca acgttacaat ttgcaaaatt ttacaaaaat gagaccgctt
      301 gtattagctg gaaagcataa aaattcagta aaatagcacg ttttagaata aaaaagagtt
      361 gaaaatggcg aagaaattat tagtaatggc aggtggaaca ggggggcacg ttttccctgc
      421 aattgccgta gctcgagagc tacaaaaaca aggctggcaa atccgctggc tggggacgaa
      481 agaccgaatg gaagccactc ttgtgccgaa acacggtatt gagattgatt ttattgaaat
      541 ctcaggcttg cggggtaaag gcgttgcagc attgttgaaa gcaccgtttg ctatttttaa
      601 agcggtgatg caagcccgca aaatcattaa aaattatcag cctgatgcgg tgttgggaat
      661 gggcggttat gtgtccggtc cgggcggtat tgcggctaaa ttatgtggcg tgccggtgat
      721 tttacacgag caaaacgcag ttgccggcct aaccaatgtg tggctatcca aaattgcccg
      781 caaggtgtta caggctttcc ctactgcttt taaagaggcc gaagtagtag gtaatcctgt
      841 acgtgaagat ttatctgctc ttccgctgcc gaatgaacgt ttcgccgaac gaggctaccc
      901 gattaacatt ttagtaatgg gtggcagcca aggggcaaga gtgatcaacc aaaccgtgcc
      961 ggaagtggca aaagtgttgg gtaataatgt gtttatcagc caccaagtcg gtaaaggtaa
     1021 tttagccggt atcgaagaag tctatcaaca aaccggcaat gggattgctt ccgagtttat
     1081 tgatgatatg aaagccgcgt atgagtgggc agatttggtg atttgtcgtt ccggtgcgtt
     1141 aacggtgtgt gaaattgccg ccgtaggcct gcccgctgtg tttgtgccgt tccaacataa
     1201 agacaggcaa caatttttaa acgctaacta tttggcggca gatggagcag cagtgattat
     1261 cgaacagagc gatttcactc caaaggcatt acagcaagcc ttagaaccct taattgccaa
     1321 tcgccaactc ttattggata tggcaatgcg agccagagca aaatccaccc caatggcagc
     1381 aaaacgtgta gcggaagtga ttattcaaga agcgaattaa cttactacaa gcggtcgttt
     1441 ttggtaaaaa ttttgcaaaa tgtaaccgct tgcgaataga ttaaaaggac aataaatgaa
     1501 aaatttccaa gataaagtca aaaaattagt gcctgaaatg cgtcgtgtca gccaaattca
     1561 ttttatcggg attggcggag ccggtatgag cggcattgcg gaagtgttat taaacgaggg
     1621 ataccaaatt tcaggttcgg atattgcaga cagagcggtt acacaacgtc tatccgcagc
     1681 cggagcgaaa gtctttatcg gacaccaagc agaaaatatt gctggggcga gtgtggtggt
     1741 ggtttctact gcgattgatg aaaccaaccc tgaaattatt gccgctcgag aagctcgaat
     1801 tccggtgatt cgccgagcag aaatgcttgc cgaaattatg cgttttcgcc acggcattgc
     1861 gattgcaggc acacacggta aaaccactac aaccgcaatg atttcaatga tctacaccga
     1921 agcaaaatta gatccgactt tcgtgaatgg cggtttggta aaatctgccg gcaaaaatgc
     1981 tcatttgggg gcgagccgtt atttaattgc cgaagccgat gagagtgatg cttctttctt
     2041 gcacttgcag ccaatggtgt cggtagtaac caacattgag cctgaccata tggacaccta
     2101 tggtggcgat tttgagaaaa tgaaagagac ttacgtacga tttttgcgta acttaccttt
     2161 ctacggctta gcggtaatgt gtgccgatga tgaaacggta atggaaattg ctcctcgtgt
     2221 cggcagacaa gtgattacct atggctttag cgaaaatgcg gattaccgaa ttgaagatta
     2281 ccagcaaacc ggcttccaag agcattacac cgtagtttgc ccaagcggtg aacgaattga
     2341 tgtgctgttg aatgtgccgg gtatgcacaa tgccttaaat gcaaccgctg cgttagcggt
     2401 ggcaaaagaa gaagggattg gcaatgaggc gattttagct gctttagctg atttccaagg
     2461 ggcaggacgc cgctttgacc aattaggttc atttattcgc ccgaacggca aaattatgtt
     2521 agttgatgac tacggccacc acccaactga agttgatgta accattcaag ccgctcgttc
     2581 aggctgggaa ggtaaacgtg ttgtgatgat tttccaaccg caccgctatt ctcgtacccg
     2641 tgatttattc gatgaatttg tacaagtact gtctaaagtc gatgccttga ttttattgga
     2701 tgtgtatgct gccggcgaag ctccgattgt gggggcagac agtaaagcct tagcccgttc
     2761 aatccgtaat ttaggtaagg tcgatccgat tttggtttca gataccgatc aacttggcga
     2821 ggtattagat caaattattc aagacggtga tttaattttg gcacaaggtg cgggcagtgt
     2881 aagccgcatt tctcgtggct tggctgagag ctggaaaaat taaatactct ctccccttgc
     2941 gggagagaga ccgaaaaata cttcgttcag aagtattttt cagagagagg gtatatattt
     3001 aaaggatttc aaatgagtat taaaaacgaa aaaattgccg tcttattcgg tggcgtttcg
     3061 caagagcgtg aagtttcact gaattctggt gcagcggtaa cagctgcatt aaaaagttta
     3121 ggctataacg tagaaggcat tgatactaaa gaattcccga ttgaaaaatt aaaagaaaaa
     3181 gggattcagc gtgtatttaa tattttacac ggcggtattg gtgaaaatgg cgtactgcaa
     3241 ggtgcgttag agcaaatggg tattccttac accggttgtg gcgtaatggc atcagcagtg
     3301 acgctggata aattccggac taaattatta tggaatgcag taggtttacc aacagcggat
     3361 atggtggtgg ttcagcgtgg acaagtggtc gattttgacc aaattattgc aaaattaggc
     3421 ttgccggtat ttgtgaagtc ttcgtgtgaa ggctcaagtg tgggtgtatt caaagtaaaa
     3481 accaaagaag aattggcacc ggcaattcac gaggcattaa agtttgattc cattgtgttg
     3541 gtggaagaat ttttagccgg taatgaatat tctgtaccgg tattagacgg acaagttcta
     3601 ccggcggtac aagtaattcc ggacggcgaa ttttatgact accacgcaaa atatgtgtcg
     3661 gataacaccc aatatgtagt accggcatta aatgacgaac gccaagcaga ggtcgccaaa
     3721 ttagtgaaag cggcttacga tgtggttggc tgtcgaggtt ggagccgtat tgatgtgatg
     3781 gaagatggcg aaggaaaatt ccgcttggtt gaagtaaata ccaacccggg aatgaccagt
     3841 cacagtattt tcccaaaatc ggcagcaaca gttggtattt cctttgaaaa attagttgaa
     3901 cgcgtattgg agttaagtgc ttaatgggca tcttccgcaa gaagaacagc acaaatgtgc
     3961 gtttgaatgt tcttaatcca aaaacacctg tttctccacg taaatggcta gtattcatta
     4021 agccattaat tgtgttatta tgtacactat ttatattcgg tgtgtatagt aattggcaaa
     4081 atatactcga aagttgggat aaaacaccga ttcgcgctta tgcattaaca cataaaacac
     4141 aatttacaac aaatgcagat attcgtgaaa ctttgtctaa agaacctgct ctaaaaggat
     4201 atttcggaca aaatattaaa gaggtcaaag ataagctttt atctatacct tgggttaaag
     4261 atgtggctgt tcgtaaactt tatcccgacc gattaagtat tactttgcta gaacataaac
     4321 ccgttgcaat ttggaatgat actaactttg tctcagaaca aggaaccgtc tttagtttac
     4381 cgaaagacag aatcgataaa aatggacttc cattactcta tggtccagat acggaaggaa
     4441 aaagtgtatt agaggcatgg aataaaatcc gagctgatct aaatgcccgc aacttggatt
     4501 taacctccgt ttctgtagat aatcgaggtt cttggacaat taggctttct aatcaagtag
     4561 aattaagatt aggaagaggg gaatggactt ctaaaattga tcgttttgta acaatctttc
     4621 ctgaaattca tatacctgaa ggtaatagat tagcttatgt tgatttacgt tatccacatg
     4681 gcgcggcggt tggatttagt cctataaaaa attaaactca gtgaacacaa aaacattatg
     4741 acaaaagcag tagaatcaaa aattatcgtt ggtcttgaca ttgggacatc aaaggttgtt
     4801 gccgttgttg gtgaagtatt acctgatggt gtaattaatg ttatgggagc tggcgtttgc
     4861 ccttctaaag gagtcgatcg aggtggcgtt attgatcttg acgctgccgt taattctatt
     4921 caaagagcca ttgagcaggc ggaatctatt gccgattgtc aaattatggg tgtaacactg
     4981 gcaatctcag gtcagcatat tgttggttta aatgaagcag gatcatcaac attatctgga
     5041 ggtattgtta cccaagataa tattgaaagt gctgtggata tggcaaaggc aattaaatta
     5101 caagatggct tagaaacatt acacatgatt ccacaagaat attacgtgga tagattgcct
     5161 gcgactaaaa atccaattgg cttatcgggt atgcgtttac aagttcaagc acacttaatt
     5221 gcttgccatc aagcttggtt aaaaaactta aaaaatgcgg ttgaacatgc taaattaaaa
     5281 gtcgatcaag ttgtattttc aggtcttgcc tctagctatt ctgttttaac tgaagatgaa
     5341 aaagagctgg gtgtttgctt aattgatatt ggtggcggta cgatggatgt actagtttat
     5401 accgatggag cattaagata cagtaaagtc attccatttg caggtaataa cattactgat
     5461 tatttggctc gtgtatttac gacatctcgc cctgaagctg aaagccttaa agtaggttat
     5521 ggtagcgcaa tttcgcctcc aacccataat tcagataaaa aaattgaggt ggcaggacta
     5581 ggtggacgga tggcaagaat ctttacccga gctcaggtgg caacggttac ctcacaatgt
     5641 tataacgatt tgctaaaagt tgttgaagag gaattaaccc aattacgcca tgaacttttc
     5701 caaaaaggaa taaaacaaga gttaattgcc ggtgtagtca ttactggtgg tggctcacaa
     5761 attgaagata ttgtagaatg tgctaaatca atttttggtt cacaagttcg tgttggatat
     5821 ccactcaata ttactggctt aaccgattat gtgaataaac cacaatatgc aacagcatta
     5881 gggcttttac aatatagcca ctataattat actgaacaag gtacaagtac agattctaat
     5941 gaagatatct ttgctcctat tggtcgtgga accaagaaaa tcattgactt cttcaaatca
     6001 aaattttaat agtgttaaaa taaacacgca gggagataaa aatgtttgaa cctattattg
     6061 aacaaacgca agatgctatc attaaggtag tgggtgttgg tggtggtggc ggtaatgccg
     6121 tagacagaat gtctcgctct gccgatgata ttaaaggtgt tgaattcttt gatgtaaata
     6181 ctgatgctca agttttaaga aaaagaacca cacgccaaac tatccaaatt ggagcaagta
     6241 caactaaagg tttaggtgca ggtgctgatc caatggtagg aaaacaagct gccgaagaag
     6301 atcgtgaggc tatcgctaat gcattaaaag gtgcaaatat gacctttatt gctgttggta
     6361 tgggtggagg taccggtacc ggagcagccc ctgttgttgc tcaaattgcc aaagagcaag
     6421 gctcactaac tgtaggcgtt gtaacaaaac ctttccgctt cgaagggcct cgccgtatgc
     6481 gctttgcaga ccaaggcatt aaagagcttt ctcaatatgt tgattctctt attataattc
     6541 ctaatgataa attacgtggt ttaggtaagc aaacaacagc agtagatgcc tttgccgcag
     6601 caaatgatgt tttaagtaac tgtgtgcttg gtattaccaa tatgattacc agttcgggcg
     6661 gttctaccgg tgcagatatt aacgtggact tcgcggatgt tcgtacagta atgtctggta
     6721 agggacacgc tatgattggt acaggttttg ctgaaggtga agttggtgaa ggacgagcgg
     6781 aaaaagcaat gaatgatgcc atttctagtc cattacttga aaatgtggat atttcaggtg
     6841 caagtggtat gctcatcaac attagtgctg ggacagattt cttattagaa gaagtttatg
     6901 ctatgatgga cttaatttat ggctttgcaa cagaagatgc cgcaattgta tttggttgta
     6961 actattaccc tgaaatggat ggaaaagtga gtgtcactct tgtcgcaaca ggaataggcc
     7021 aacctgagga agctctacat atgccacata aggctcagcc agtatatgca cagcaaggaa
     7081 atcatgttca agctacacag cctactcagc caaaccatgc ggtacaacaa ccaaacttta
     7141 ctcaaccgtc aagttatggt cagccaaatc aatttagtgg tcaaccacaa ccactacaac
     7201 aacctgtggc tccaaaacca caatcggttg atacagcaca aatttttaac ccaaattcaa
     7261 ttccgggttt tatgagaaat agtcaataac ttccactcat ttcggaagta aggacaaaat
     7321 atgactaaac aaagaacatt aaaacaagca gcaaaagtaa caggtattgg cttacatagc
     7381 ggcaaaaaag taaccttaac gttacgccct gccccagcta atacaggagt tatttatgcc
     7441 cgtaccgatt tagagcctgt cgtttatttc cctgcaagcg cagactctat tcgtgataca
     7501 caattatgca catgtatggt aaatgatgat ggtgttcgta tttcaacagt agaacattta
     7561 aacgcagcaa tgtcagcatt aggcttagat aatgttattg ttgaagtaga tgcacctgaa
     7621 attccgatta tggacggtag ttcaagccca tttatctatc ttttattaga tgccggaatc
     7681 gaagaacaag atgcaccgaa gaaatttatt cgcattaaag aaacagtacg cgttgaagaa
     7741 ggcgataaat gggccgaatt aaaaccatac aataaaggtt ttaaattgaa cttcactatt
     7801 gactttgagc acccattaat ccccaaaaat gtagggagct attccattga tttttctgct
     7861 gagaacttta ttcagcaact aagtcgagcg agaaccttta catttatgaa ggatgtagaa
     7921 tacttgcaat ctattggtct tgcattaggt ggaagccttg ataatgctat tgtattagat
     7981 gattaccgaa ttctaaatga agacggttta cgatttaaag atgaactggt tcgccataaa
     8041 atgctggatt cgataggtga cttatatatg tgtggttata atatcctagg tgaatttagt
     8101 gcatataaat caggtcatgg cgttaataat aaattattaa gaactgtatt ggaaaataaa
     8161 aacgcttggg agtttgttac ctttgaagat aaagcagaag tcccacaagg ttaccaagta
     8221 caacaagaaa tttttattta gtctaaatgg cttaaaagac gaacagaaat gttcgtcttt
     8281 tattttgcaa attttttcta atatttaacc gcttgcaacc cgcctcatta gcgatttttt
     8341 cgcccttatc tatttactgt ttaaaaaaac agttgtacta tatacccata cagttaatct
     8401 tgtgtgatgt attatgctta ctcaacttac tattaataat tttgcgattg ttcgccatct
     8461 tattcttgaa ttaaatgaag gtatgtctgt tattaccggt gaaaccggtg ccgggaaatc
     8521 aatcgctatt gatgccttag ggctatgctt gggttatcgt tctgaaagtg ggatgattag
     8581 aaacggttcg gacaaagcag atataagtgc aacctttact atgcagccac atagccctgc
     8641 ttatttatgg ctacaagagc atgaactgct tgatgaagat aatcctcaag agtgtatctt
     8701 acgccgtatg attaatattg atggacgctc aaaagccttt gtaaataacc gatcattacc
     8761 tgtttcacaa cttcgagagt tggggcaata tcttattcat ttaaatgggc aacacgcacc
     8821 acaattactc ctaaaaaacg aatatcaact tgaaatagtc gatcattatg ccacgttaca
     8881 gcctctattg atgaagatgg caaagcaata tgggaattgg aaagaactga ataagcaggt
     8941 aaaaaatttc caaaaacagt gccaagaaaa tgaagctcgc aaacagctat tagaatatca
     9001 agttgaggaa ttagatgaat ttgcaattaa agcaaacgaa tttgaagaaa tggaagagaa
     9061 atacaatatc ttgtcttctt ccgaggagct tactgagctt tcagatgaaa tcacacgctt
     9121 attaagtgat gatgaaatca atgcaaacag tctgatttat aaagcgttac gcaatgcaga
     9181 aaacttggct gaggttgata gccaatatca gggcatttta gatatgctta atgaggccct
     9241 gattcagatt caagaagcaa gctccgaagt ggctcaatta gccagtaaaa ttgaacaaga
     9301 tccggaatta ctacaggagc tggataatag aattgcacaa actatatcgt tagcacgcaa
     9361 acaccatatt caaccgaatg aattatggca atatcacgcg acattaaaag cagaactaca
     9421 ggaattactg gattttgaag ataatgaaga acaactcatt caaaatgaaa aacaagctta
     9481 ccaagagtgt ataacattag cagaagaaat ttaccaaaaa cgtctagatg ccgcacataa
     9541 gctctctgaa caagtaacaa aacaaattaa acacttagca atggaaaacg gtgagttctt
     9601 tattcatata gaacataatc ttgaaaaaat gtctgcaaat ggtgctgact atgtcgagtt
     9661 taatttacgc agcaatttag gacaacaagc ccaaccactg gcaaaaattg cttctggtgg
     9721 tgagctatct cgcatttctc tggcagtaca agtgcttacc gcaaataaac tttctacgcc
     9781 aactattatt tttgatgaag ttgatgttgg gattagtggc tctgctgcca ctacagtcgg
     9841 aaagctactg cgtaagctag gcgaaaaatg ccaagtatta tgtgtaaccc atttacctca
     9901 agtggcaagt tacggcaacc agcagtttaa tgtacaaaaa tatgtagaaa ataatcaaac
     9961 tgaaacgcaa atgacattac tcacagaagc agagcgtatt cacgccttgg ctcgactatt
    10021 aggcggaagc gaaatcaccg aaacggtttt agctaatgca aaagaaatgc taaatttagc
    10081 gaaaaataac gaatagccaa aaagaaaaat cgcatcaatg aatattgatg cgattatgaa
    10141 tttggtggag gtaaacggga tcgaaccgat gacctcttgc atgccatgca agcgctctcc
    10201 caactgagct ataccccctt agatacaagc ggaataatat gctttccgct tgaagatgtc
    10261 aatggcttta atcaagcaag gtgttgattg attaaaaact agctattcgc ctgaataaac
    10321 tcaatcgctt tttgtaagcg agctaaggtt ctttctttac cgactaattt ggcggtgata
    10381 tccattgacg gcgattgccc acttcctgtt accgctaaac ggaacggcat accgactttg
    10441 cccataccga tttccagctc ggcagcggtt tggttcattg cctcgtggat attttccacc
    10501 gtccagtcat tgcaagcggt caatttttcc aataattttg caagtggagc aacggcttcg
    10561 gctttgaagt ttttagcggc agctttttcg tcgtaagcct taaattcttg atagaaatag
    10621 cggctgcttt cggcaagctc ttttaaggtt ttgcaacgtt cgcccagtac agggataatt
    10681 tcttccaatt ttggaccgct tgtgaaatca atgccttgag tttgcatatg ccattcaaga
    10741 tgttttgcca cataacttgg ggcaagactg cggatatagt gttggttcaa ccattgtaat
    10801 ttttcagtgt taaatgcact tgccgattta ctgactgaat gaatgtcgaa cagctcgatc
    10861 atttcgtcac gagagaaaat ctcttggtcg ccgtgtcccc agcctaaacg cacaaggtag
    10921 ttaattaagg cttccggtag atagccgtca tcacgatatt gcattacgct caccgcaccg
    10981 tggcgtttgg agagtttttg cccgtcatcg ccgttgatca ttgaaacgtg ggcgtaggtt
    11041 gggattggtg ctcctaatgc tttcaaaatg ttgatttggc gtggtgtatt gttgatatgg
    11101 tcttcacccc gcacaacgtg agtaatgccc atatcccagt cgtccaccac cacacagaaa
    11161 ttgtaggtcg gcgagccgtc agtacggcga ataatcaagt catctaattc gctattggcg
    11221 atttcaattc gcccacgcac cgcatcgtca aatactaccg agccttcggt cgggtttttg
    11281 aaacgcacaa catgtggttg gctcgggtcg ttttgcggat tgtgtaagca gtgctgatcg
    11341 tatttcgctt tctctttatt tgcttcttgc tctgcacgta cttcgtctaa acgttctttc
    11401 gagcagtagc aacggtatgc cgtgccggct tctaacattt ggtcgatcac ttggttatag
    11461 cgatcgaaac gcttggtttg aaagtatggg ccgtgttccc acgccaaatc cagccattcc
    11521 ataccctcta aaatagcggc ggttgcctca ggggttgagc gttcaagatc ggtatcttca
    11581 atacgtaaca caaattcacc atcattatgc ttagcatata accaagagta gagcgcggtc
    11641 ctcgccccac ctacgtgcaa gtagccagtt gggctaggtg caaaacgggt acggactttt
    11701 acattcggat ctaaggggaa aagggtttca attttcattt tatattgcct aattgatgat
    11761 tcttaagaaa aacttatcta ttttactacg cttgagggtg aatcatagta aaaaattaaa
    11821 tttgcggggt aaaaaatcag caaacggggt aaaaagaaca aaaagggttt gacgaaacag
    11881 caaaaatctc tataattcac ttccacaaca
//
LOCUS       DANJWC010000068        11898 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.068, whole genome shotgun sequence.
ACCESSION   DANJWC010000068 DANJWC010000000
VERSION     DANJWC010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11898
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.068"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..115)
                     /locus_tag="PWX02_001329"
     CDS             complement(<1..115)
                     /locus_tag="PWX02_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HDL1729342.1"
                     /translation="MLFNLKFWQKPAKIITDSDSFRFQLFNDPYLGYVKADN"
     gene            complement(518..1621)
                     /gene="glf"
                     /locus_tag="PWX02_001330"
     CDS             complement(518..1621)
                     /gene="glf"
                     /locus_tag="PWX02_001330"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HDL1729343.1"
                     /translation="MRKYDYLIVGVGLFGTIFAYEATKRGKKCLVIEKRDHIGGNCYT
                     QNIEGINVHKYGAHIFHTSNKLVWDYIQQFAEFNRFTNSPVARYKDELYSLPFNMLTF
                     NKMWGVIKPEEAEAKIKEQIAQEHITEPKNLEEQAISLVGRDIYEKLIKGYTEKQWGR
                     KCTELPAFIIKRLPVRYTYDNNYFYDTYQGIPIGGYTGIFERMLDGIEVKLGVDFFLE
                     REYYENLADKIVFTGMIDEYFGYRFGRLEYRSLRFDNELLDIPNFQGNAVVNYTEAEV
                     PYTRIIEYKHFEYGIQPKTVMTREYSKEYEQGDEPYYPINDARNNELYAKYKVLADEK
                     SNVIFGGRLAQYKYFDMHNIIAEALACVDKHFK"
     gene            complement(1848..2696)
                     /locus_tag="PWX02_001331"
     CDS             complement(1848..2696)
                     /locus_tag="PWX02_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL1729344.1"
                     /translation="MDAKVEDRRITDLKARISALNLISMVNFTDRISVNWGDYSQIQV
                     EMLLFKKAHRNSSYAYYHLLSGVDLPLVSQDTIHQFFDKHQGKEFLTIVSKESFERNK
                     IYDRVRIHYWAPHISERTFSNKLIGKFFQKFIRSSELYMKRICSADKFKTFGTALGYA
                     SQWVSITDELVTILLDEELWIRDVFSHSLFCDELFIPTIINKYGWESRIYYSKGMVND
                     PEEFQGNLRYINWWDGSPYTWTDSSEDFSSLEKAINRGHFFARKFDIQTYPAMIDFIK
                     ARTQFS"
     gene            complement(3012..3347)
                     /locus_tag="PWX02_001332"
                     /pseudo
     CDS             complement(3012..3347)
                     /locus_tag="PWX02_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249521.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chain length determination protein"
     gene            3496..3837
                     /gene="erpA"
                     /locus_tag="PWX02_001333"
     CDS             3496..3837
                     /gene="erpA"
                     /locus_tag="PWX02_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005706127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HDL1729345.1"
                     /translation="MSDIQIPLIFTDAAARKVKSLIEGEENPNLRLRVYITGGGCSGF
                     QYGFTFDDQVNEGDLTIENQSVGLVVDPMSLQYLIGGTVDYTEGLDGSRFVVNNPNAS
                     STCGCGSSFSI"
     gene            complement(3942..4265)
                     /gene="yidD"
                     /locus_tag="PWX02_001334"
     CDS             complement(3942..4265)
                     /gene="yidD"
                     /locus_tag="PWX02_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDL1729346.1"
                     /translation="MVKTHSPEQKAGTTESINQPPRLSLLAKILLLPIYFYRYAISPL
                     IGPRCRFYPTCSTYAVEAIKIHGAIKGGYLAFKRILRCNPLSEGGEDPVPLCCKGNCK
                     HNDKK"
     gene            complement(4214..4588)
                     /gene="rnpA"
                     /locus_tag="PWX02_001335"
     CDS             complement(4214..4588)
                     /gene="rnpA"
                     /locus_tag="PWX02_001335"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDL1729347.1"
                     /translation="MKKLNFSRELRLLAPVQFKAVFEEPLRASTPQMTLLARENNLEN
                     PRLGLTVAKKHLKRAHDRNRIKRIVRESFRLKQHELPNFDFVFVAKGGIGKLDNATLF
                     ETLEKLWSRHIRLSKKPAQQNP"
     gene            complement(4624..4758)
                     /gene="rpmH"
                     /locus_tag="PWX02_001336"
     CDS             complement(4624..4758)
                     /gene="rpmH"
                     /locus_tag="PWX02_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDL1729348.1"
                     /translation="MKRTFQPSVLKRARTHGFRARMATKNGRQVLARRRAKGRKSLSA
                     "
     gene            4995..5957
                     /gene="hemH"
                     /locus_tag="PWX02_001337"
     CDS             4995..5957
                     /gene="hemH"
                     /locus_tag="PWX02_001337"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDL1729349.1"
                     /translation="MNQPQQIGILLANLGTPDEPTPSAVKRYLKQFLSDPRVIDLPKF
                     KWQAILNCIILPRRSPKVAKLYQSVWSEEGSPLLAISRQQQRAVQRYFDAKGKNVVVE
                     LGMSYGNPSIESATDNLIKQGVERIIVLPLYPQYSSTTTASVFDAFANGLKAQRKIVP
                     FDFIHSYHNDPLYIQALANSIDVKPDELLLFSFHGIPKRYETEGDFYAKHCQETAELV
                     AEKLGLPREKWYLSFQSRFGDEEWLQPYTDETLESFPQKGVKKVAVVCPGFSADCLET
                     LEEIAEENRENFEHTGGESYRYIPALNASGDHIKALVKLIEAKI"
     gene            complement(6021..6968)
                     /locus_tag="PWX02_001338"
     CDS             complement(6021..6968)
                     /locus_tag="PWX02_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDL1729350.1"
                     /translation="MPTKPHKEYHELVELLISRGMLINDKSYAEKKLSQVGYYRLSGF
                     WHISRKPNQDGGLSENFLENVQFEEVYKLYIFDKKLRLLLLDIIERLEVNIRNIMAHE
                     LGRENPLAYEDYSLINQKFLKFQWKSNYAYANWLETTANKIKRSKDDSIEWHRKNEKE
                     IPFWVITETWDFGALSKFYSLLNGQHQRKIAKKFNVDENTLKNWLNEINIVRNLSAHH
                     SRIWNRKFSDIAIPKLDDPRWSKFKKMQQYFSNLSLDDKAKERIFGRVAILWFLVSQT
                     STNYHWLDKFEDLLNDFPNLPNTKVELMGFNREILEKIK"
     gene            complement(7180..9297)
                     /gene="spoT"
                     /locus_tag="PWX02_001339"
     CDS             complement(7180..9297)
                     /gene="spoT"
                     /locus_tag="PWX02_001339"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HDL1729351.1"
                     /translation="MYLFEPLDRIIQGYLPPEQIERVKRAYVIARDAHEGQTRSSGEP
                     YITHPVAVASIIAEMKLDQEAVIAALLHDVIEDTPYTEEELAAEFGQSVADIVQGVSK
                     LDKLKFRTRQEAEVANFRKMILAMTKDIRVVLIKLADRTHNMRTLGALRPDKRRRIAK
                     ETLEIYSPLAHRLGIEHIKNELEDLGFEAMHPQRYRVLKMAIASARGHRKELIQRIAD
                     DIRGRLEEVQIEARVYGREKHLYSIYQKMRQKDQHFHSILDIYAFRVVVDNVDNCYRV
                     LGQMHALYKPRPKRVKDYIAVPKPNGYQSLHTSMIGPHGVPVEVQIRTEDMDKMADMG
                     VTAHWRYKEGESHHNTTVQLKAQQWLQSIVELQSSAGNTAEFIDSVKSDLFSDDIFVF
                     TPKGRIVELPANATAIDFAYAVHSGIGDRCVGAMVDRNPYPLSEPLRTGQTVEIITEQ
                     GKRPNASWLNFVVSGKARAKIRQSLKNQQHDEAIALGRRQLVRAMNLRSLDELCSETI
                     QSVLDELRLSSFDELLAEIGLGNQMSNVIAQRLLGEPLLIDTDGNPENNQPLKIEGIE
                     NRLISFANCCLPIPGDEIIAHVSSGKGLVIHNACCGNVKDYATEPSNYLPVEWEIAKE
                     KAIQFETEIVLDIINEPGVLAGITSTLAKLNSNIGSINSEAREGNIYQVRVQISVENK
                     EHSELILRKLLSVKGVVKAARKQ"
     gene            complement(9407..9688)
                     /gene="rpoZ"
                     /locus_tag="PWX02_001340"
     CDS             complement(9407..9688)
                     /gene="rpoZ"
                     /locus_tag="PWX02_001340"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL1729352.1"
                     /translation="MARVTVQEAAEKIGNRFDLILIAARRARQLQLHTREPLVPEEND
                     KSTVIALREIEEGLITTDIMDQLENHDSIKQEAAEQEAISFLTEMSNEA"
     gene            complement(9791..10288)
                     /locus_tag="PWX02_001341"
     CDS             complement(9791..10288)
                     /locus_tag="PWX02_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL1729353.1"
                     /translation="MAHFYEYLEFNSDEDRENQLEVYVEVKLSTDTEEKLQAVGVQGD
                     WLVMAEPYCPDCVEIVAYFQRMAKLNPNIKVKYVARKDNQERKHFDSDEQQQAVISAQ
                     KIPTIFDIRNGKTELVLSEFPQFLKDKMAANPEQFEQLKADFRMGKFGKEVEAELLAI
                     LLNTK"
     gene            complement(10313..11335)
                     /locus_tag="PWX02_001342"
     CDS             complement(10313..11335)
                     /locus_tag="PWX02_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1729354.1"
                     /translation="MSVTKKRLTLSDIAELSGVSKTTVSMILNGRGNDFRIKSETCQK
                     VKEIAKKHGYRANVYAKSLQAQRTNVIGLVIPDLTNYGFALTSRTLEKLCRENGLQLV
                     IACSDDTFEGEKKAIERLLDRQVDLLITAPTHNEPTYYQNAAQYTPMLQLDRYISGLN
                     IPSITSNDTQSISMLVENIVRAYHLKEFFYLGGQLSLSPSQNRLKGFREGLEQSHLAL
                     DENWVLHQDYQPESGYQMFAQIVEKLGRLPEAVFTASFTLLEGVMRYLTEHQQMDKLL
                     TQELHLATFDDHHLLNALPFHIHSIKQDHQQIAERTFELIQQKLAQQKIDDVKVDCEI
                     LWRKSI"
     gene            complement(11375..>11898)
                     /locus_tag="PWX02_001343"
     CDS             complement(11375..>11898)
                     /locus_tag="PWX02_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GH32 C-terminal domain-containing protein"
                     /protein_id="HDL1729355.1"
                     /translation="LPDLTYPTDKYQWHSMLTLPRKLSLQNGKVIQNPIVNVGELQAV
                     EMSENFAIENLDTAYLQISVENQPLVLDFFSNEQGQKLSLRFENGVLSLDRSQTEQTE
                     LMEKFGSVRHCKIEQLETIEIFFDRSVVEIFVNGGEKVLTSRFFIAKRENVVKSSRPI
                     QVKVAKVLPISVE"
BASE COUNT         3642 a         2416 c         2030 g         3810 t
ORIGIN      
        1 tattatctgc tttaacataa cctaagtaag gatcattaaa aagctgaaat ctaaaagaat
       61 cactatctgt aataatctta gccggcttct gccaaaattt taaattaaac aacatctttt
      121 taacaaaaat cttaaagaca ggaattgtaa ttgaagatat acagaatatt agtattaatg
      181 tacttcttga ataaaaatcc gcattctgcc ccaaagctaa tgctgcaaac aaaataataa
      241 aagataaaaa gcaagctcta accactaact tgctttcgtg ccaaaactca aatcttctat
      301 tgtagacacc aaaataagca agaagaagaa taagagtaac atatacggca ctaaatgtaa
      361 tataagaata tgttattacc ggcatatccc aaaaaacatt taataccttt cttaatgata
      421 ctgcacatat tattgaaaca agtatggcaa atatatcaaa aataattaat gttatcgcag
      481 aaattatctt attcaaaata ttccccttgc aaattatcta cttaaaatgt ttgtctacac
      541 aagctaaggc ctcagcaata atattgtgca tatcaaaata tttatactga gctaaacgtc
      601 cgccaaaaat cacattgctt ttttcatcag ctaatacttt atatttagca taaagctcat
      661 tatttcttgc atcattaatt gggtaatatg gttcatctcc ttgttcatat tctttcgaat
      721 attcacgggt cattactgtt ttaggttgga taccatattc aaaatgttta tattcaataa
      781 ttcgggtata aggaacttct gcttccgtat agttaactac cgcattacct tgaaagtttg
      841 gtatatctaa taattcgtta tcaaaacgta aacttcggta ctctaatctg ccaaaccgat
      901 aaccaaagta ttcatcgatc attccggtaa acacaatttt atcagctaga ttctcataat
      961 attcacgctc aagaaagaaa tctacaccaa gttttacttc tataccatct agcattcttt
     1021 caaaaatgcc cgtataacca ccaatcggaa taccttgata agtatcatag aagtaattgt
     1081 tatcgtaagt gtaacgcaca ggtagacgtt taataataaa agcaggcaac tctgtacatt
     1141 ttctacccca ttgtttttcc gtatagcctt tgattaattt ttcataaata tcacgaccaa
     1201 ctaatgagat tgcttgttct tccagatttt taggttctgt aatatgctct tgagcaattt
     1261 gctctttaat tttggcttca gcttcttccg gtttaattac tccccacatt ttattgaagg
     1321 ttaacatatt aaatggcaag ctatagagtt catctttata acgtgcaacc ggtgaattgg
     1381 taaagcgatt aaattcagca aattgttgaa tgtaatccca aaccaattta ttgcttgtat
     1441 ggaaaatatg agcaccatat ttatgtacgt tgataccttc tatattttga gtatagcagt
     1501 tacctccaat atggtcacgt ttttcaatta ccaaacattt tttaccacgt tttgtggctt
     1561 cataagcaaa aatagtccca aaaagaccta ctccaacaat aagatagtca tattttctca
     1621 taagaatgcc ctaatttata ttaactaata tctaaattta ttatcatcta tagctataac
     1681 tttcttccct aaaattagtc tacttaggaa atgatttctg aactcaccag aacttacttc
     1741 ttttaagccc tctttcatac tgtaatttat ccaggcataa aacaaaaaac actcattcca
     1801 tagtcttaat tacgaaaaga attagtaaga agtattaact cataaattta agaaaactgc
     1861 gttctcgcct taataaaatc tatcattgct gggtaagttt gtatatcaaa ctttctagca
     1921 aagaaatgcc ctctattaat agccttctct aaagaggaaa aatcttcact cgaatctgtc
     1981 caagtgtaag gagaaccatc ccaccaatta atatatctta aattaccttg aaattcctcc
     2041 ggatcattta ccatcccttt tgaataataa atcctagatt cccacccata tttattaata
     2101 attgtaggaa taaataattc atcacaaaat agtgaatgag aaaaaacatc tctaatccat
     2161 aattcttcat cgagcaggat agtaactagc tcatctgtaa tgctaaccca ctgtgaggca
     2221 tatcctaaag ccgtaccgaa agttttaaat ttatctgcac tacatattct tttcatatac
     2281 aattctgaag atcggataaa tttttgaaaa aatttaccaa tcaacttatt actaaaagtt
     2341 cgttcactaa tatgtggtgc ccaatagtgg attctgactc gatcataaat tttatttctt
     2401 tcaaatgatt ctttcgaaac tatagtcagg aattctttac cttgatgttt atcaaagaat
     2461 tggtggatag tatcttggct gactagaggt aaatcaacac cagatagcaa atgatagtaa
     2521 gcatagctgc tatttctatg agctttctta aagagaagca tttcaacctg tatttgagaa
     2581 taatcgcccc agtttacaga aattctatca gtaaaattta ccattgatat caaattcaga
     2641 gcactgattc ttgctttcaa atcagtaata cggcgatcct ctactttagc atcaataaat
     2701 aaatatcatt tctaggatga ttaagtaact caattaaaaa gagtaattgc tcaaattttt
     2761 catgtgcaat tataagataa gcatgctttt tagacataat aaacctgttt ttgatgaata
     2821 caactagatt ttctcacaaa gctctaatta gccttttcag aagctaaagc atacaagata
     2881 actctaatcc cagcagatat aaatgaattg aagtatcctt catattttat gacaactatt
     2941 ttttaatcaa attaaatatc tctgttagaa gtaataaaag ataatcttgc ttatttaagt
     3001 aaagtgataa aaaattcatt tttagtatct tgagattgtg ctaatcggtt aaaattgttg
     3061 aacaaactcg cagacaatgc atttggatca acaggatcta cacgcaaaat ccgactatat
     3121 tctctgcgaa catttaaata ctcacctaac tctgttaatt taggggcaat aactgttgcc
     3181 ttagacgtcc attgctcttt tgcagtaaat gcataaatac cagcaagtaa tgtggtaatg
     3241 aatgttgtta taacaatcca ccatttttta tcccatagag cacgaaaaat ctcaattaaa
     3301 tcaatctcat ccgagctttg ctcatgactt tttactgaat ctgccatagt ttcctctaaa
     3361 atttgcatgt ttctgtacag cattatacat aattttctta tttccactgc attcatttac
     3421 tcttttttaa gattgcactt cctatgtaat tattagataa tatccaatca cagtaaaatt
     3481 ttgatctaaa acaaaatgag cgatattcaa attcctttaa tctttactga tgcggcggct
     3541 cgtaaagtaa aaagcttaat tgagggcgaa gagaacccga atcttcgctt gcgtgtgtat
     3601 attacgggtg ggggctgtag tggattccag tatgggttca cctttgatga tcaagtcaat
     3661 gagggagacc ttaccattga gaatcaaagc gttggcttgg tggttgaccc gatgagccta
     3721 caatatttaa ttggtggcac ggtggattat accgaagggc tggacggctc acgttttgtc
     3781 gtgaataatc caaatgcaag ctcaacctgt ggttgtggtt cttcctttag catttaattc
     3841 actcgaatta ttgccttatt caatatcagt ctcacaagta aattttttac taaaaatgac
     3901 cgtttgtatc aaacaaaata gcctaactta ttgagtttag gctatttttt atcattatgt
     3961 ttgcagttgc ctttacaaca aagtgggaca ggatcttcgc ctccctcact caaagggttg
     4021 caacgcagaa tacgcttaaa cgctaaatac ccacctttaa ttgctccgtg gattttaatc
     4081 gcttcgaccg cataggtgga gcaggttggg taaaatcggc aacgtgggcc aatcaatggg
     4141 ctgatagcat agcgatagaa ataaatcggt agcaatagaa ttttggctaa taggctgaga
     4201 cgaggtggct gatttatgga ttctgttgtg ccggcttttt gctcaggcga atgtgtcttg
     4261 accatagttt ttccaatgtt tcaaacagcg ttgcgttgtc taatttgcca atgccgcctt
     4321 ttgccacaaa tacaaaatcg aaattcggca attcgtgttg cttgaggcga aaactttcac
     4381 gcactatccg cttaatgcga ttacggtcgt gagcccgttt taaatgtttt ttggcaacag
     4441 ttaagcctag acgaggattt tctagattat tctctctagc aagaagagtc atttggggtg
     4501 tgctggctcg aagtggctct tcaaacaccg ccttaaattg aacgggagct aacaaacgta
     4561 gctcccgaga gaaatttagc ttcttcacac ttaatcagaa aggtgttttg gctggttctg
     4621 caattatgca gataaagatt tacgaccttt agcacgacga cgcgctaaca cttgacgacc
     4681 gtttttagta gccatacgag cacggaaacc gtgagtacga gcacgtttta atactgatgg
     4741 ttgaaatgta cgtttcattg cttatctacc taaaatttaa aaagttggca taaaatggtt
     4801 tctgccaaac aaggaccata aaaaagagtt gcaattctac aggcaattaa aataaaaagc
     4861 aagaaacaat ccaagcacaa ctcgggttgt ttgcaaaatt tttgaaaaat acgaccgctt
     4921 gttataatcg cagaaattta tattggtttg tggcataatc gaaaaaatat taactattga
     4981 tgagaaatag aacaatgaat caaccccaac aaataggtat tttgctggct aatttaggta
     5041 cgccagatga acccacgcca agtgcggtga agcgttattt aaagcaattt ttaagcgatc
     5101 ctcgtgtgat cgatctacca aaatttaaat ggcaagcaat cctaaactgc atcattttgc
     5161 caagacgttc gccaaaagtg gcaaaattat atcaatcagt atggagcgaa gaaggctcac
     5221 cgttattggc gatttctcgg caacaacaac gagcggtaca acgctatttt gatgccaaag
     5281 gcaaaaatgt ggtggtggaa ttgggaatgt cttacggcaa tccaagtatt gaaagtgcga
     5341 ccgataacct catcaagcag ggggttgaac gaattattgt gctaccgctt tatccgcaat
     5401 acagtagcac tactaccgct tcggtgtttg atgctttcgc caacggctta aaagcacaac
     5461 gtaaaatcgt gccgtttgat tttattcaca gttatcataa tgatccgctt tatattcagg
     5521 cattagctaa ctctattgat gttaaacctg acgagttatt actgttttca tttcacggca
     5581 ttcctaaacg ttatgaaacg gaaggtgatt tttacgccaa gcattgccaa gaaacagcag
     5641 aattagtggc tgaaaagcta ggtctgccaa gagaaaaatg gtatttgtct ttccagtccc
     5701 gttttggtga tgaagagtgg ctacagcctt ataccgatga aacgcttgaa agttttccac
     5761 aaaaaggggt aaaaaaagtg gctgttgttt gcccgggctt ctccgccgat tgcttagaga
     5821 ccctggaaga aattgcagaa gaaaaccgtg aaaactttga acacaccggc ggagaatctt
     5881 atcgctacat tccagcgtta aatgcgagtg gcgatcatat caaggcatta gtaaaattaa
     5941 tcgaagcgaa aatataatat aatttgggcg gataataaat ccgcccattt ttatctacac
     6001 cctataggaa tgtacttatt ttatttaatt ttctctaata tttctctgtt aaatcccatc
     6061 agttctactt ttgtattcgg taaattaggg aagtcattta ataaatcttc aaacttatct
     6121 aaccaatggt aattagtgct ggtttgacta actaaaaacc acagaatagc tacccttccg
     6181 aaaattctct cctttgcttt gtcatccagc gataagtttg aaaaatactg ctgcattttt
     6241 ttaaatttcg accaacgagg atcatctagc ttaggaatag caatatcgct aaattttctg
     6301 ttccaaatac gagaatggtg agcagaaagg tttcttacaa tgtttatttc attcaaccaa
     6361 ttctttaacg tattctcatc tacattaaat ttcttagcaa ttttcctttg gtgttgccca
     6421 tttaacaatg agtaaaactt agataatgca ccaaaatccc aggtttcagt aattacccaa
     6481 aaagggattt ccttctcatt ttttctatgc cattcaatag aatcatcttt actacgctta
     6541 attttattag ctgtagtttc tagccaattt gcataagcat aattagattt ccattgaaat
     6601 tttaagaact tttgattaat cagagaataa tcttcataag ccaaaggatt ttcacgcccc
     6661 aattcatgag ccataatatt acgaatatta acttctaaac gttcaataat atccaataat
     6721 aaaagcctta gttttttatc aaaaatatat agcttataaa cttcttcaaa ttgtacattt
     6781 tcgagaaaat tttcgctaag cccaccatct tgattaggct ttctagatat atgccaaaat
     6841 ccagataaac gataataccc tacctgagat aattttttct ctgcatagct tttatcattt
     6901 atgagcattc ctctagatat gagtagttct actaattcgt gatactcttt atgtggtttt
     6961 gtaggcataa aattgaaggc attttaagaa aggcaaggac gtagcttgac actctaagtc
     7021 gattacattt agtaaacctc agggacaggg gctacgccac gattgcaagg gattatacat
     7081 tttagctgat taaagtcaaa aggtttcatc aattaataat agcaatactg tacttttaag
     7141 gactataatt gcaaaaaact gataaaattt taccgcttgt tactgcttac gggccgcttt
     7201 tacaacacct tttacactta atagcttacg gagaatcagc tcagaatgct ctttattttc
     7261 cacagaaatt tgtactctca cttgatagat atttccttct cttgcctcgc tattaatgct
     7321 gccaatgttg ctatttaatt tggcaagcgt tgatgtgatc cccgcgagta cccctggttc
     7381 attgataata tccagtacga tttccgtttc aaattgaatc gctttttctt tggcaatttc
     7441 ccattccaca ggtaaataat tactcggctc tgttgcgtaa tctttcacat taccacaaca
     7501 agcattgtgg attaccaatc ctttacctga actgacgtga gcaataattt catcacccgg
     7561 tataggtaag caacagtttg caaaactgat aaggcggttc tcaatccctt caattttaag
     7621 cggttgatta ttttctggat taccatcagt atcaattaat agtggctccc ctaataagcg
     7681 ttgagcgata acattactca tttggttacc tagaccaatt tcagccagta gctcatcaaa
     7741 gcttgataaa cgtagctcgt ctaatacgga ttgaatagtt tctgaacaca attcatcaag
     7801 tgagcgtaaa ttcattgccc gaactagctg tctacgccca agtgcaatcg cctcatcgtg
     7861 ctgctgattt ttaagcgatt gacgaatttt agccctcgcc ttaccactta ctacaaaatt
     7921 taaccacgat gcatttgggc gcttaccttg ctcggtaata atttccaccg tctgtcctgt
     7981 tcgtagcggt tctgaaagtg gatacggatt gcgatcaacc atcgcaccta cacaacgatc
     8041 cccaatgcct gaatgcaccg cataggcaaa gtcaatagcg gtagcattcg ccggcaattc
     8101 aacaatacgc cctttcgggg taaagacgaa aatatcgtca gagaacagat ccgatttaac
     8161 gctatcaata aattctgctg tattgccggc actgctttgc aattcaacaa tactttgtag
     8221 ccattgctgg gctttgagtt gaacggtcgt gttatggtgc gactcgccct ctttgtaacg
     8281 ccagtgagct gttaccccca tatccgccat tttgtccata tcttcggtgc gaatctgtac
     8341 ctccaccggt acaccgtgcg ggccaatcat cgaagtgtgt aaagattgat agccattcgg
     8401 ttttggcacg gcaatataat ctttcacccg tttcggacga ggcttataca gtgcgtgcat
     8461 ctgccctaat acacggtaac aattatctac attatcaacc actactcgga aagcataaat
     8521 atctaaaatc gaatgaaaat gttgatcttt ctggcgcatt ttttggtaaa tggaatagag
     8581 atgtttttca cgcccataca cacgggcttc aatttgcacc tcttctaacc gaccacgaat
     8641 atcatcggca atgcgttgaa ttagctcttt acgatgtcct ctggcactgg caatagccat
     8701 tttcagtacc cgataacgtt gcgggtgcat tgcctcgaag cctaaatctt ccaactcgtt
     8761 tttaatatgc tctattccca agcgatgtgc caacgggcta taaatctcta aggtttcttt
     8821 cgctatacgg cggcgtttat ccggacgcaa agcaccaagc gtacgcatat tatgggttcg
     8881 gtcggcaagt ttaattaaca caacccgaat gtctttggtc attgccaaaa tcattttacg
     8941 gaagtttgcc acttctgcct cttggcgggt gcggaatttt aatttatcca gcttagaaac
     9001 gccttgtaca atatctgcta cgctctgtcc aaattccgcc gccagttctt cttcggtgta
     9061 aggtgtatct tcaatcacat cgtgcaacaa agccgcaatc accgcttctt gatcgagctt
     9121 catttccgca ataatcgacg ccactgctac cgggtgagta atataaggct caccgctaga
     9181 gcgtgtttgc ccctcgtgag catcacgggc tatcacataa gcacgtttta cacgctcaat
     9241 ctgctcgggt ggcaaatagc cttgaataat tcgatctaaa ggttcaaaaa gatacacgtt
     9301 atacctcaac aacagaaata aaaatggaca acgaatttgc aaattttgag gaaaatccta
     9361 ccgcttgcag actcaattgt ccattctcta tttacgaaaa ataaaattac gcttcgttgc
     9421 tcatttcagt taaaaatgaa atcgcctctt gctccgccgc ttcttgctta atagagtcgt
     9481 gattttccag ctgatccata atgtcagttg taattaagcc ttcttcaatt tcacgtaatg
     9541 caatcacggt cgacttatcg ttttcttcag gcacaagtgg ctcacgggtg tgaagttgta
     9601 attgtcttgc acgacgtgca gcaattaaga ttaaatcaaa acggttgcca attttctccg
     9661 ctgcttcttg tacggttaca cgagccataa atttactcca aagatttatt cggttttatt
     9721 cataaaaaga ttgcgaatta tacagaaatt ccgccaattc cgcaatgaaa gcgggctaaa
     9781 ttcacaaaat ttatttggta ttcagtaaaa tcgccaataa ttcagcctca acctctttgc
     9841 cgaattttcc cattctaaaa tcggctttta gttgctcgaa ttgctcaggg ttcgctgcca
     9901 ttttatcttt taaaaattgc ggaaattcag ataacactaa ttctgttttg ccattacgaa
     9961 tatcaaaaat agttggaatt ttttgcgcag aaatgaccgc ttgttgctgt tcatcgctgt
    10021 caaaatgttt gcgttcctga ttatctttgc gagccacata tttcacctta atgttaggat
    10081 tgagctttgc cattcgctgg aaataagcca caatttccac acaatcgggg caatatggct
    10141 ctgccattac cagccagtcc ccttgcacgc caacagcttg cagtttttct tccgtatctg
    10201 tggaaagctt gacctccaca tacacttcta attgattttc acgatcttca tcgctgttaa
    10261 attctaaata ttcgtaaaaa tgtgccataa ttttgtcttt tcctatctca tcttaaatgg
    10321 atttacgcca taaaatttca caatcaactt taacgtcatc tattttctgt tgcgctaact
    10381 tttgctgaat taattcaaag gtacgttcgg caatttgttg gtggtcttgc ttaattgaat
    10441 gaatatgaaa aggtagtgca ttcagtaaat ggtgatcatc aaaggttgct aaatgcagtt
    10501 cttgagtcaa taatttatcc atttgttggt gttcagtcag atagcgcatt actccctcaa
    10561 gtagggtaaa agaggctgtg aataccgctt ctgggagcct gccaagtttt tcaactatct
    10621 gagcgaacat ttgatagccc gattccggct gatagtcttg atgcagaacc caattttcat
    10681 caagtgccaa atgtgactgc tccaaacctt cacggaagcc ttttaaacga ttttgacttg
    10741 gcgagagtga aagttgtcca cctaagtaaa aaaactcttt taagtgataa gcccgtacaa
    10801 tattttccac caacatactg atactttgtg tatcattact ggtgatagaa gggatattca
    10861 acccagaaat atagcggtct agctgtaaca ttggggtata ttgtgccgca ttttgataat
    10921 aagtcggctc attatgtgtc ggagcagtaa tcagtaaatc tacctgtctg tctaataaac
    10981 gctctatcgc tttcttctca ccttcaaagg tatcatccga gcaagcgatc actagctgta
    11041 acccgttttc acggcataat ttctctaagg ttcttgaggt cagagcaaag ccataattcg
    11101 ttaaatcggg aatgactaaa ccgatcacat tcgtgcgttg agcctgtaat gattttgcat
    11161 atacattagc acgataaccg tgttttttag cgatctcttt cactttttgg caggtttcgg
    11221 atttgattct aaaatcattg ccccgcccat ttaaaatcat actgactgtt gttttagata
    11281 cgccgctgag ttccgcaata tcactgagcg ttaggcgttt tttagtcaca cttatctcct
    11341 tttagcataa aaatgggcat tactgcccat tgctttattc tacgctaatt ggcaatactt
    11401 ttgctacttt cacttgaatc ggacgagagc tttttacgac attttcacgc ttagcgataa
    11461 aaaagcgtga agtcagcact ttttccccgc cgtttacgaa aatttccacc acagaacggt
    11521 caaagaaaat ctcgattgtc tctaattgtt caattttgca atgacgaacc gagccaaatt
    11581 tttccatcaa ttctgtttgt tcggtttggc tacgatctaa gctcaacacc ccattttcaa
    11641 aacgtaagct gagcttttgc ccctgttcat tgctgaaaaa atccaacaca agcggttgat
    11701 tttccacaga aatttgcaag taggcagtat ccaaattctc gattgcaaaa ttttctgaca
    11761 tttcgaccgc ttgtaattcg cctacattca cgattgggtt ttgtatcact ttgccgtttt
    11821 gcagggaaag tttacgaggc aatgtcagca ttgaatgcca ctgatattta tcagttgggt
    11881 aagtgagatc aggcaagc
//
LOCUS       DANJWC010000069        11873 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.069, whole genome shotgun sequence.
ACCESSION   DANJWC010000069 DANJWC010000000
VERSION     DANJWC010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11873
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.069"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..285)
                     /locus_tag="PWX02_001344"
     CDS             complement(<1..285)
                     /locus_tag="PWX02_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HDL1729356.1"
                     /translation="MKKIFWILLIAVLAVGGYSLTKYNDLMRAEEDINSVWGNVESAY
                     QRRADLIPNLVNTVKGQANFEKETLTSVIEARAKATAVTIDPSNATEEQMA"
     gene            458..1321
                     /gene="rarD"
                     /locus_tag="PWX02_001345"
     CDS             458..1321
                     /gene="rarD"
                     /locus_tag="PWX02_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDL1729357.1"
                     /translation="MLRGILASLAANVLFGAVYYFAILLRPLEGEAMFGFRIVVVIPF
                     ILLAIILFKQQSAFADLLQKIRQNPPLVLVILLLALNTGVQLWLFLWAPNHGQALQVS
                     FGYLLLPIVAVALGKMVFKEHFTRLKWLSLLFAVIGVGSSLFFNSTISWATWLAGLGY
                     PLYITLRRYFQINTLATFFVELLLVLPVALYYLSQVEMTPIVAENPQIYFYLGLLGLV
                     SGSAFILYILSSNLLPINVLGLLGYVEPLVMLLISFAIGEKLDANSYILMVCLLVSIG
                     LLSWDSFRSKV"
     gene            complement(1474..1992)
                     /locus_tag="PWX02_001346"
     CDS             complement(1474..1992)
                     /locus_tag="PWX02_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lhr family helicase"
                     /protein_id="HDL1729358.1"
                     /translation="MILLRGDTMQFIEFTDPDSGISYQYSEFTIANVAFIINFCSDAD
                     VISTLSALGKDITNYINTYSCCTIKFMAKEHLENSGSNIDIYAPAANHQFKRKEIIAL
                     QETLERLLFEHYVRFTPESYLFIAERDSLNRMYQHMCVPRCDFMQSFQVVYPLGVNQD
                     CFILITPKGNLK"
     gene            complement(2159..3823)
                     /locus_tag="PWX02_001347"
     CDS             complement(2159..3823)
                     /locus_tag="PWX02_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL1729359.1"
                     /translation="MNISFVFPQTLGNARRFLVNEMLSAAAKQQGHNVVAANQADFVV
                     LFDNAIPQEAAGKQGAILDLEQAFAQPEASLKQAVENAQTFANATATTAVSAVSSGVK
                     NIVAVTACPTGVAHTFMSAEAIENYAKAQGWNIKVETRGQVGAGNPISAEEVAAADLV
                     FVAADIDVDLEKFKGKPMYRTSTGLALKKTTQEFEKAFAQAKIYEGGVAKADNAEVPT
                     SEKKGLYKHLMTGVSHMLPLVVAGGLLIAISFMFGIEAFKDETIAGGLPKALMDIGGG
                     AAFHLMIAVFAGYVAFSIADRPGLAVGLIGGMLATTAGAGILGGIVAGFLAGYTVKFL
                     NGAIQLPASLTSLKPILILPLLGSAIVGLLMIYVINPPVKALMDALVEWLNTMGQTNA
                     IILGIILGAMMCTDMGGPVNKAAYTFGVGLIASQQYMPMAAVMAAGMVPPIGMAIATL
                     IARSKFNTNQRDAGKASFVLGLCFISEGALPFVAADPVRVIASSILGGATAGAISMAL
                     GITLQAPHGGLFVIPFVSQPLMYLAAIAIGSLVTGVVYAAIKQKAE"
     gene            complement(3849..4793)
                     /gene="fruK"
                     /locus_tag="PWX02_001348"
     CDS             complement(3849..4793)
                     /gene="fruK"
                     /locus_tag="PWX02_001348"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL1729360.1"
                     /translation="MRIATVTLNPAFDLVGKLQRIEIGEVNTVETLGLYPAGKGINVA
                     KVLADLGTKLSVTGFLGEENQGDFVNAFKHNDLADEFYRINGKTRINVKITETEADVT
                     DLNFLGFEISESDWEKFVTLSHQWESKFDLVAVCGSLPRGVTPEKFASWLESLHRQGL
                     KVVLDTSNAALTAGLTAHPWLVKPNRRELEVLVGHSLNSIEEIISAAKQLRSRGIENV
                     IISMGEQGSLWLNHEGVLQARPPRCENVVSTVGAGDSMVAGLIYGLSQGWSKEQTLRF
                     ASATSALAVVQSNVGIADRATLDDIIKQTDITVISENH"
     gene            complement(4807..6285)
                     /gene="fruB"
                     /locus_tag="PWX02_001349"
     CDS             complement(4807..6285)
                     /gene="fruB"
                     /locus_tag="PWX02_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HDL1729361.1"
                     /translation="MLNLSAKNIRLNGSASNKEEVIRFVAAELVAKGNVAAGYEVGML
                     NRETQTSTFLGNGIAIPHGTLDTRDLVKETGVQIIQLPQGVEWGKGNTAYVVIGIAAK
                     SDEHLTLLRQLTTVLSDEEAAATLAKTQNLDEFIAVLSGKKNLPALSQELISLNIESS
                     SLITLSAINAAKLQEQGYVSQAFISDVVANKALHLGNNLFVVDSAQGNQANGIAIARN
                     TQGQTLLTVAETDNALQPLLTQLLKPEVRSQFAHFSAEQMLGLLKGESVASTPVTNVE
                     ASGNQVVGTFTIRNDNGLHARPAAVLVQTVKPFSSKISVENLDRNTAPASAKSAMKVV
                     ALGASQGHRLRFVAEGEDAQQAIEALQQAIINGLGESVSFVPAVADTIESIAVSAIPA
                     EETATAVDANTKEATFVIKNEHGLHARPSAVLVNEVKKFNAKIEVQNVDKNSPLVSAK
                     SLMKIVALGVTKGTTLRFVASGDDAEVALAAIGAAIEAGLGE"
     gene            6573..8795
                     /gene="glgB"
                     /locus_tag="PWX02_001350"
     CDS             6573..8795
                     /gene="glgB"
                     /locus_tag="PWX02_001350"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HDL1729362.1"
                     /translation="MQDYTYSEKDAFFVERLSSGSCNDPFSYLGIHPSNNGVIIRVFL
                     PSAIACQVIDLNGKPVSSMLKIDDRGFFIAELIGKKTDFSYRLLVEYPDLTVDIEDPY
                     RFKTHIQELDNWLFAEGKQLRPYEKLGAHLVTQNEVGGVHFSVWAPNAQRVSVVGDFN
                     HWNGLVHSMRFHPDSGIWDIFLPNVKKGACYKFEVLDRNGVLRLKSDPYAFASQFRPE
                     TASIVSGLPSIVPAEKTCRNANAPDKPISIYEVHLGSWRRNIENNYWLSYEELADELI
                     PYVKDMGFTHIELLPISEFPFDGSWGYQTTGIYSPTSRFGTPEGLSYFIQKAHDKGIG
                     VILDWVVGHFPVDEHGLVYFDGTHLYEHSDPKEGYHKDWNTLIFNYGRNEVSNYLSGN
                     ALYWIERFGIDALRVDAVASMIYRDYSRSDGEWIPNKYGGRENLEAIDFLRHTNNILR
                     DESQGGASIAEESTSFAGVSHSTERGGLGFNYKWNMGWMNDTLRYMQKDPIYRRYHHN
                     LMTFGMTYQYSEQFVLPLSHDEVVHGKGSLLNKMPGDCWQKFANLRAYYGYMWGYPGK
                     KLLFMGNEFAQGKEWNFAESLDWYLLGEEGGEWHKGVLGWVKALNYLYTQTPALFEQD
                     YNPEGFEWLVMDDHDNSVFAFERKAKNGDSVIVVSNFTPIVRENYRFGVNELAEYQEI
                     LNSDLACYCGSNVSNANRFASELIASHGKAYSLSITLPPLSTIFLKRAADKKTTQNIT
                     "
     gene            8814..10823
                     /gene="glgX"
                     /locus_tag="PWX02_001351"
     CDS             8814..10823
                     /gene="glgX"
                     /locus_tag="PWX02_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HDL1729363.1"
                     /translation="MHYFRGKPFPLGNKLDRQAGEFGTNFAIVSQNATAIELCLFDEN
                     HHETRIPMFSSGDTWHIFVAGVQAGTKYGYRVKGIQDEQAGLLFNPQKLLTDPYAKAI
                     EGKPDLSSAEAAAWYKWNDERDNASLAPKSIVIDEHFDWENDSQLFTPWAETIIYEMQ
                     VKGFSQRNFQIPSDIRGTFAGLVHPVSIDYLKTLGITAVELLPVYHIDETHLQKQGLV
                     NYWGYNVLGNFAIDPNLAADKQNPLKEFKQLVKTLHQAHIEVIMDIVFNHTAESDKNG
                     PMLSFRGIDNTAYWLDKQGEYENWTGCGNTLNLSNPYILCWAIDCLIYWVEECHIDGF
                     RFDLASILGRTPSFSEKADFFTALKSHSKLTHIKLIAEPWDIGAEGYQLGQFPEPFYQ
                     WNDCYRDDIRRFWLQNQGDWGLLARRLAGSDDVFAKYSVSKSINFITAHDGFTLQDLT
                     SYQQKHNWANGEQNRDGHNSNYSNNHGVEGQTADELVKKQREITACSLLATLFLSAGV
                     PMLLAGDEIGHSQQGNNNSYCQDNEITWLDWENMNHTRLDYVRSLIRLRKKIALLSKQ
                     NNWWQEDSVQWLNPSAQLMNIDDWHNQHNGLVVLLQEEWLLLFNANRQPQPFLLPQGN
                     WKVVLEQAQGTLMTDTQAVTLNNMGVCVLQKSDVMFANKLETQYA"
     gene            10816..>11873
                     /gene="glgC"
                     /locus_tag="PWX02_001352"
     CDS             10816..>11873
                     /gene="glgC"
                     /locus_tag="PWX02_001352"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDL1729364.1"
                     /translation="MLNQESTALVARQDLTNDTLVLILAGGRGSRLYELTDKRAKPAV
                     YFGGSRRIIDFALSNCINSNLLKIGVITQYAAHSLLRHLQRGWSFLPYERNQYIDMLP
                     ARQQLDENTWYRGTADAVYQNMAMMKSHYRSKYVVILAGDHIYKMDYTQMLKDHVDQG
                     AKCTVGCIEVPRSQATEFGVMAVNEKLKVKEFVEKPADPPAMPGKPDSSLASMGIYVF
                     NADYLYEMLEKEASTPGTSHDFGKDIIPKAVEQGVLYAHPFERSCKGRNASGAIYWRD
                     VGTIDSYWAAHMDLVSEDPELDLYDESWPIHGRPQQTAPARFFYNRARARTLDNSLIA
                     GGCVITDAEISNSVLFNRV"
BASE COUNT         3302 a         2361 c         2643 g         3567 t
ORIGIN      
        1 agccatttgc tcttcggtgg cattgcttgg gtcaatagtt accgcagtcg cttttgcacg
       61 agcttcaatt acagatgtta aggtttcttt ctcaaagtta gcctgacctt ttacggtatt
      121 cactaagttt ggaattaaat ccgctcggcg ttggtaagca gattcaacat tcccccatac
      181 ggagttgatg tcttcttctg cacgcattaa atcgttgtat tttgttaaag aataaccgcc
      241 aactgcaagc acggcgatca gtaaaatcca aaaaatcttt ttcattatga aatcctataa
      301 ataaaagagt gaaaaattgg gtgcattgta tcgggaaaaa gcggatatag ctagcgtact
      361 tacaaatttt ttcatactat ggggcgtaga taagatttgt aaatttttgt tcaaagtcga
      421 ccgcttgttg tttgattgta gataagagga aagccaaatg ctcagaggaa ttttagcttc
      481 gcttgcagcc aatgtgctgt ttggagcagt ttactatttt gcgatattac ttcgcccgtt
      541 agaaggagaa gcgatgttcg gctttcggat agtggtagtc attccgttta ttttgctggc
      601 gattatattg tttaaacagc aatcggcttt tgcggatctt ttgcaaaaaa ttcgacaaaa
      661 tccaccgctt gtattggtca ttttgctcct tgctctcaac accggcgtgc agctttggct
      721 gtttttatgg gctcctaatc acgggcaggc gttgcaggtg tcgtttggtt acttgctttt
      781 accgattgtg gcggtggctc taggaaaaat ggtgtttaaa gaacatttta cccgcctgaa
      841 atggctttct ctacttttcg cagtcatcgg tgttggcagt agtttgtttt ttaacagcac
      901 gatttcgtgg gcgacttggt tagctgggtt gggttatccg ctttatatta cccttcgccg
      961 ttattttcaa atcaacacgt tagctacttt ttttgtggag ttgttactgg tattacccgt
     1021 tgcactttat tatctcagcc aagtggaaat gacgccaata gtggcagaaa atccgcaaat
     1081 ttatttctac ctcggattgc tggggctggt aagtggcagt gcttttattc tttatatcct
     1141 ctcgagcaat ttattaccga ttaatgtatt aggtttactg ggctatgttg aaccgttggt
     1201 gatgttgctg atttcttttg ccatcggcga aaaactcgat gccaattcct atattttgat
     1261 ggtctgtttg ctggtttcga ttggattatt gagttgggat agttttcgta gcaaggtgtg
     1321 ataattaagg aaaaataaat cgcttgtaga gcaagcggtt taaagattat ttgaccggtt
     1381 tcaatagacc tgcttttttt gctcgctgat aggcttgctt aaaagcgatt aagccttctt
     1441 gctttaaacg aacggaaact ggacgttgtt gattcatttt agatttcctt ttggcgtaat
     1501 taaaatgaaa caatcttgat taacgcccaa gggataaacc acttggaaag attgcataaa
     1561 atcacatcgt ggcacacaca tatgctgata cattcggtta agtgaatctc tctcggcaat
     1621 gaagagatag ctttctggtg taaaacgtac ataatgctca aataataaac gctcaagggt
     1681 ttcttgtaaa gcaattattt ctttacgttt gaactgatga ttcgctgcgg gggcataaat
     1741 gtcaatatta gaaccgctat tttctagatg ctctttcgcc ataaatttga ttgtgcaaca
     1801 ggaataggtg tttatatagt ttgtgatgtc tttgcctaat gcggacaaag tcgatataac
     1861 atcggcatct gagcagaaat taatgataaa tgcgacatta gcaatagtaa attcactgta
     1921 ttgataagaa attccactat caggatcggt aaattcaata aactgcatag tatctcctcg
     1981 tagcaaaatc ataacgagtt gatattattc gtaaagtgct gaaaatgatt ttttcgatct
     2041 cgatcgcaaa aataatattt tagacttgat atttcgatga aatagaattc agtaagtacc
     2101 aatatgatat aaaaacggag cgtttcgctc cgtttttgtt tgtatttgtg tttgtgcttt
     2161 attctgcttt ttgtttgata gcggcataga ctacgccggt gactaatgag ccgatggcaa
     2221 tagcagctaa gtacattaac ggttgtgaaa cgaatggaat cacgaataaa ccgccgtgtg
     2281 gggcttgtag ggtaatgcct agtgccattg aaattgcacc ggcggttgca ccgcctaaga
     2341 ttgagctggc aattacacgc acagggtctg ccgccacgaa cggtaatgcc ccttctgaaa
     2401 taaagcataa acctaacacg aatgaagctt tacctgcatc acgttggttg gtgttgaatt
     2461 tgctacgagc aatcagggtc gcaatcgcca taccaattgg tggaaccata cctgccgcca
     2521 tcaccgctgc cattggcata tattgttgtg aagcaattaa acccacacca aaggtataag
     2581 ccgctttatt caccggacca cccatatcgg tacacatcat cgcaccgaga ataataccta
     2641 aaataattgc attggtttgc cccattgtgt ttaaccattc cactaaggca tccattaatg
     2701 ctttcaccgg tgggttgatg acataaatca tcagtaagcc gacaatcgct gagcctaaga
     2761 gtggtaaaat caaaataggt ttaagtgagg ttaaacttgc cggtagctga attgcaccat
     2821 ttaagaattt tacggtataa ccggctaaga aaccggctac gataccacct aaaatccctg
     2881 ctcctgcggt ggtagcaagc ataccgccga taaggccgac agctaacccc ggacggtctg
     2941 caattgagaa cgctacatag cctgcgaata ccgcaatcat taagtggaat gccgcaccgc
     3001 caccaatatc cattaaggct ttcggcaagc cgcctgcgat ggtttcatct ttaaaggctt
     3061 caattccgaa cataaaggaa atagcgatga gcaagccacc tgctaccacc agcggtaaca
     3121 tatgtgatac accggtcatc aagtgtttat ataacccttt tttctcagaa gttggaactt
     3181 cagcattatc cgcttttgcc acaccacctt cgtaaatttt cgcttgagca aaggcttttt
     3241 caaattcttg agtggttttc ttcaatgcta aaccggttga ggtgcgatac atcggcttgc
     3301 ctttgaattt ttccaaatcg acatcaatat cggcagctac aaagactaaa tctgccgctg
     3361 cgacttcttc ggcggaaatt ggatttcctg ctcctacttg accacgggtt tcaaccttga
     3421 tgttccaacc ctgtgctttg gcataatttt cgattgcttc cgcagacata aaagtatgag
     3481 ccacgccagt tgggcaagcg gtaacagcaa cgatattttt cacaccagat gaaaccgctg
     3541 aaaccgcagt ggttgcggta gcatttgcaa aagtttgtgc attttcgacc gcttgtttga
     3601 gactggcttc aggttgagca aaggcttgtt ctaagtccaa aattgcccct tgcttgccgg
     3661 cagcttcttg tggaattgcg ttatcgaata acaccacaaa atcagcttgg tttgccgcca
     3721 caacattgtg tccttgttgc ttggcagcgg cagataacat ttcattcact aagaaacggc
     3781 gtgcgttgcc tagtgtttga ggaaatacaa aagaaatatt catattggtt tgttcctttg
     3841 attgaggttt aatgattttc tgaaatcacg gtaatgtcgg tttgtttaat aatatcgtct
     3901 aacgtcgcac ggtctgcaat cccaacgtta ctttgaacaa cagctagagc agaagtagct
     3961 gaggcaaaac gcaaggtctg ctctttgctc caaccttggg ataagccgta gatcaagccg
     4021 gcaaccattg aatcaccagc acctacagtg ctaactacat tttcgcaacg tggtgggcgg
     4081 gcttgtaaca cgccttcgtg atttagccaa agagagcctt gttcgcccat tgatataatt
     4141 acattttcaa taccacggga acgtaattgt ttagctgcac taataatctc ttcgattgag
     4201 ttgagtgaat gcccgaccag cacttccagc tctcggcggt ttggttttac taaccaaggg
     4261 tgagcggtta agccggcggt gagagccgca ttgctggtat ctaacaccac ttttaagcct
     4321 tggcggtgta aagattccaa ccaagaagca aatttttccg gcgttacgcc acgagggaga
     4381 gatccacaca ctgcaactaa gtcaaatttg ctttcccact gatgagataa tgtgacgaat
     4441 ttttcccaat cgctttccga aatttcaaag ccgagaaagt tcagatcagt tacatcagcc
     4501 tcggtttcag tgattttgac attaatgcgg gttttaccgt taatacggta aaattcatca
     4561 gccaaatcat tgtgtttaaa ggcatttacg aaatcgcctt gattttcttc gcctaaaaag
     4621 ccggtaaccg aaagttttgt gcctaagtca gctaaaactt tagcaacgtt aatacctttg
     4681 ccggcagggt ataaaccaag ggtttctacg gtgttcactt cgccgatttc aatacgttga
     4741 agtttgccga ctaaatcaaa ggcagggttg agggtaacgg ttgcaatacg cataatgatt
     4801 cctcgcttat tcgcctaaac ctgcttcaat cgcagcaccg attgccgcaa gtgctacttc
     4861 ggcatcatca ccgcttgcca caaagcgtag cgtcgtgcct ttggttacgc caagggcaac
     4921 aattttcatt aagcttttgg cacttacaag cggtgaattt ttatcaacat tttgcacttc
     4981 aattttggcg ttgaattttt tcacttcatt caccaacacc gcacttgggc gagcgtgtaa
     5041 accgtgttcg tttttaatta caaaagttgc ttctttcgta ttggcatcga cagcagtcgc
     5101 agtttcttcc gcaggaattg ctgaaacagc aatgctttca atcgtatctg ccaccgccgg
     5161 tacgaatgaa acactttcgc ctaagccgtt aataattgct tgttgtaatg cctcaatcgc
     5221 ttgttgggcg tcttctccct ctgccacaaa gcgtaaacga tgcccttggc ttgcccctag
     5281 tgctactact ttcattgcac ttttcgcact tgccggtgca gtgttgcggt ctaaattttc
     5341 aacgctgatt tttgaggaga acggtttcac cgtttgtact aacaccgcag ccgggcgggc
     5401 gtgtaagccg ttgtcgttac gaatggtgaa tgtgccaacc acttggttgc cactcgcttc
     5461 gacattggtt acaggggttg aagctacgct ttcgcctttt aataaaccaa gcatttgctc
     5521 cgcagagaaa tgagcaaact ggcttcttac ttctggtttt aataattgag ttaatagtgg
     5581 ctgtagagca ttgtcggttt ctgccacagt tagtagggtt tgaccttgcg tattacgagc
     5641 aatagcgata ccgttagctt ggttgccttg ggcggaatcc accacaaata gattattgcc
     5701 taaatgtaac gctttgttgg caaccacatc gctgatgaat gcttgggaaa catagccttg
     5761 ctcttgtagt tttgcggcgt taatggctga taaagtgata aggcttgaag attcgatatt
     5821 cagagaaatc agctcttgag ataaggcagg aaggtttttc ttaccgctta atacggcaat
     5881 aaattcatct aaattttgcg ttttcgctaa tgtggcagca gcctcttcat cacttaatac
     5941 agtggtgagt tgacggagta aggttaagtg ttcgtccgat ttagccgcaa tcccgataac
     6001 tacataagca gtgttgcctt tgccccattc cacgccttgc ggaagttgga taatttgcac
     6061 gccggtttct ttgactaaat cacgggtatc aagcgtgccg tggggaatag caattccgtt
     6121 acctaaaaaa gtagaggttt gggtttcacg attaagcata ccgacttcat agccggctgc
     6181 tacattgccc ttggcgacta attcggcggc aacaaaacgg ataacttctt ctttgttgct
     6241 tgctgaacca tttaaacgga tgttttttgc cgataaatta agcattttaa ctccttggat
     6301 ttaaagttga tttttaaact gtaatgttat ggttagttta ctctatattt tgctaaaccg
     6361 ttatagccaa ctaaaaatat ccataaaaat gtttcaaaat gtgatctatg tcacaaaatt
     6421 taaggtttga tgatttaaac taaagattaa aacgatttta ttttgtgatt tagctcaaaa
     6481 aatggcgtaa atccacttct tttatttcta tttagtgata gagtgagaac tattgaaatt
     6541 taagtattaa acgctttaag caaggagtgc cgatgcaaga ctatacttat tctgaaaaag
     6601 atgctttttt tgttgagcga ctttcttcgg gttcttgtaa tgatcctttc tcctacctag
     6661 gtattcaccc tagcaataac ggcgttatta ttagggtatt tcttccttct gctatagctt
     6721 gccaagtgat cgatttaaac ggaaagcctg tgtcatcaat gctaaaaatt gatgatagag
     6781 gcttttttat tgcagagctg attggtaaaa aaacagattt ttcttaccgc ttgttggtgg
     6841 aataccccga tttaacagta gatattgaag acccttaccg ttttaaaacc cacattcaag
     6901 agctggataa ctggctgttt gccgagggaa aacaacttcg cccgtacgaa aaattggggg
     6961 cacatttagt cacgcaaaat gaggttggtg gtgtgcattt tagcgtatgg gcaccaaatg
     7021 cacagagggt ttctgtggtt ggggatttca accactggaa cggtttggtg cattcaatgc
     7081 gattccaccc cgattccggc atttgggata ttttcctgcc gaatgtgaaa aaaggggctt
     7141 gctataaatt tgaagtgttg gatcgaaacg gcgtgttgcg tttgaaatct gacccttacg
     7201 cctttgcctc ccaattccgc cccgaaaccg catctattgt ttccggcttg ccaagcattg
     7261 tgccagccga gaaaacctgc cgtaatgcga atgctcccga taaaccgatt tccatttatg
     7321 aagtgcattt aggctcttgg cgacgcaata ttgaaaacaa ttattggctt agctatgaag
     7381 aactcgctga tgaactcatt ccttatgtga aagatatggg ctttacccat attgaattgc
     7441 tgcctatttc cgaatttccg tttgatggct cttggggtta tcaaaccacc ggcatttatt
     7501 cgccaaccag ccgttttggt acgcctgagg gcttgagtta ctttatccaa aaagctcacg
     7561 ataaaggcat tggtgtgatt cttgactggg tagtggggca ttttccggta gatgaacacg
     7621 gcttggttta ttttgatgga acgcatttat acgaacattc cgatccgaaa gagggctacc
     7681 acaaagattg gaatacctta atttttaact acggcagaaa tgaggtgagc aattatttgt
     7741 ctggtaatgc actttattgg attgagcgtt ttgggattga tgcgttgcgg gtggatgcgg
     7801 tggcgtcaat gatttatcgt gattactcac gttcagacgg cgagtggatt ccaaataaat
     7861 atggcggacg tgagaactta gaggcaatcg acttcttacg ccacaccaat aatattttga
     7921 gagacgaaag ccaaggcggt gcgagcattg cagaggagtc cacctctttt gccggcgtgt
     7981 ctcactccac tgaaagaggc ggtttgggct ttaattataa atggaatatg ggctggatga
     8041 acgacacttt acgctatatg caaaaagatc caatttaccg ccgttatcac cacaatttaa
     8101 tgacttttgg tatgacttac caatacagcg aacagtttgt gttaccgctt tcacacgatg
     8161 aagttgttca cggcaaaggt tcattgctga ataaaatgcc gggggattgc tggcaaaaat
     8221 ttgcgaactt acgagcctat tatggctata tgtggggcta tccgggcaaa aaattgctct
     8281 ttatgggcaa cgagtttgct caaggtaaag agtggaattt tgccgagagc ttagattggt
     8341 atttactcgg ggaagaaggg ggcgaatggc ataagggcgt attgggttgg gtaaaagccc
     8401 ttaactattt atacacccaa acccctgcac tgtttgagca agattacaat cctgaaggtt
     8461 ttgagtggtt agtgatggac gatcacgata attcggtgtt tgcttttgag cgtaaagcca
     8521 aaaatggcga ttcggtgatt gtggtgagta atttcacccc gattgtgcgt gaaaattacc
     8581 gttttggggt gaatgaattg gcagaatatc aggaaattct caattctgat ttagcttgtt
     8641 attgcggtag taatgtcagc aatgcaaacc gatttgccag cgagttaatt gcatcgcacg
     8701 gtaaagccta ctccttgagc attactttgc caccgctatc gactatcttc ctcaaaagag
     8761 ccgcagacaa aaaaacaaca caaaacataa cttaaagaaa cataaaaaat aatatgcact
     8821 attttagagg taagcctttt cctttaggca ataaacttga tagacaagcg ggtgaatttg
     8881 gcacaaattt tgccattgtt tcgcaaaatg caaccgctat tgagttatgt ctttttgatg
     8941 aaaatcacca cgaaacccgt attccgatgt tttcttccgg agatacttgg cacatttttg
     9001 tagcaggtgt acaagcaggg acaaaatatg gctacagggt gaaaggcata caagatgaac
     9061 aagccggttt gttatttaat ccgcaaaaat tattgactga tccttacgct aaagcgattg
     9121 aagggaagcc ggatttaagt agtgcagagg cagccgcttg gtataaatgg aacgatgaac
     9181 gggataatgc ctctcttgca cctaagtcta tcgtgattga tgagcatttt gattgggaaa
     9241 atgatagcca gttattcact ccttgggcag agactattat ttatgaaatg caggtgaaag
     9301 gttttagtca gcggaacttt cagattccaa gcgacattcg aggcactttt gccggtttgg
     9361 ttcaccctgt ttcgatagac tatttaaaaa ccttaggaat caccgcagta gaacttctac
     9421 ccgtttacca tattgatgaa acccatttgc agaaacaagg gcttgtgaat tattggggct
     9481 ataatgtatt aggtaatttt gctattgatc cgaatttagc agcagataag caaaatcctc
     9541 ttaaagagtt taaacagctc gtaaaaacat tacatcaagc acatatcgaa gtgattatgg
     9601 atattgtgtt taaccacaca gctgagagtg ataaaaacgg gccgatgcta agttttagag
     9661 gcattgacaa tacagcttat tggctagata aacagggcga atacgaaaat tggactggct
     9721 gtggtaatac cttaaatttg agcaatcctt atatactctg ctgggcaatc gattgcttaa
     9781 tctattgggt ggaggagtgc catattgatg gattccgctt tgatcttgcc tcaattctag
     9841 gtagaacacc gagtttttct gaaaaagcag attttttcac agcacttaaa agccattcta
     9901 aattaacaca tattaagttg attgctgagc cttgggatat tggtgcagaa ggttatcaat
     9961 tagggcaatt ccctgagcct ttttatcagt ggaatgattg ttatcgtgat gatatacgcc
    10021 gtttttggtt acaaaaccaa ggtgattggg ggcttttggc acggcgattg gcaggaagtg
    10081 atgatgtgtt tgctaaatat tccgtttcaa aaagtatcaa ttttattacg gcacacgatg
    10141 ggtttacgtt acaagatctc accagctatc agcaaaaaca taattgggca aatggtgaac
    10201 aaaaccgaga cgggcataat agtaactaca gcaataacca cggtgttgaa gggcagacag
    10261 cagatgaatt ggtaaaaaaa cagagagaaa tcaccgcttg tagtttgctt gctacgctct
    10321 ttttatcggc tggtgttcca atgcttttag ccggtgatga aatcggacat agtcaacaag
    10381 gtaacaacaa tagttattgc caagataatg agatcacttg gctagattgg gaaaatatga
    10441 atcacacacg gctggattat gttcgtagtt taatccggtt gcgtaaaaaa atcgctttgc
    10501 tttccaagca gaataattgg tggcaagagg attcggtaca atggttgaat ccttctgctc
    10561 agctaatgaa tattgacgat tggcataatc aacataatgg gcttgtcgtt ttattacagg
    10621 aagaatggct gcttttattc aatgctaatc gacaacctca accgttttta ctgccacaag
    10681 gcaactggaa agttgtgttg gagcaagctc aaggtacttt gatgacagac acgcaagcgg
    10741 ttacgctcaa taatatgggg gtatgcgtac ttcaaaaaag tgatgtaatg ttcgctaata
    10801 aattggagac acaatatgct taaccaagaa tcaacggctc ttgttgcaag acaagattta
    10861 accaacgata ccttagtatt aattcttgca ggaggacgag gttctcgcct atacgaatta
    10921 accgataaac gtgcaaaacc ggcagtctat ttcggtggta gccgccgtat tattgatttt
    10981 gcgttatcaa attgtattaa ctcgaattta ttaaaaatcg gggtgattac ccaatatgca
    11041 gcacactctt tgttgcgcca tttacaacgt ggttggtcat ttttacctta tgaacgtaac
    11101 caatatattg atatgttacc ggcacgccaa cagctagatg aaaacacttg gtatcgtggc
    11161 acagcggatg cggtgtatca aaatatggcg atgatgaaat cgcactatag atcaaaatat
    11221 gtggtgattt tagcagggga tcatatctat aaaatggact acactcaaat gctgaaagat
    11281 cacgtcgatc aaggtgcaaa atgtactgta ggttgtatcg aagtgcctcg ctcgcaagca
    11341 acagagtttg gggtaatggc ggtgaatgaa aagctgaaag tgaaagagtt cgtcgaaaaa
    11401 ccagctgatc cacctgcaat gccgggaaaa ccggattcct cgctagcatc aatggggatt
    11461 tatgtattta atgcagatta tctatatgaa atgctggaaa aagaagcctc tacaccggga
    11521 acctcacatg atttcggtaa agatattatt ccaaaagcag tagagcaagg tgtgctatat
    11581 gcccacccat ttgaacgttc ttgtaaaggg cgtaatgcca gtggggcaat ttactggcga
    11641 gatgtcggta cgattgacag ctactgggct gcccatatgg atttagtttc tgaagatccg
    11701 gaattagatt tatatgatga atcttggccg attcacggtc gtccgcaaca aactgcaccg
    11761 gcacgttttt tctataatcg tgcgcgtgcc agaaccctag ataactcact gattgcgggt
    11821 ggttgtgtga ttaccgatgc ggaaatcagt aattcggtct tatttaaccg tgt
//
LOCUS       DANJWC010000070        11762 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.070, whole genome shotgun sequence.
ACCESSION   DANJWC010000070 DANJWC010000000
VERSION     DANJWC010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11762
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.070"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(4..333)
                     /gene="trxA"
                     /locus_tag="PWX02_001353"
     CDS             complement(4..333)
                     /gene="trxA"
                     /locus_tag="PWX02_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDL1729365.1"
                     /translation="MSNVIHTTDATFEQDVLKSDVPVLLDFWAPWCGPCRMIGPVLDD
                     LSVDAQFDGKVKIVKMNVDENPSVPAQFGVRSIPFLLLFKNGEVIAQQVGAIPHAQMA
                     EFIKQAL"
     gene            complement(580..1245)
                     /locus_tag="PWX02_001354"
                     /pseudo
     CDS             complement(580..1245)
                     /locus_tag="PWX02_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
     gene            1500..4067
                     /gene="clpB"
                     /locus_tag="PWX02_001355"
     CDS             1500..4067
                     /gene="clpB"
                     /locus_tag="PWX02_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDL1729366.1"
                     /translation="MNFEKMTTKLQEALAESQSLAVGKDNPYIEPAHLLYALVKQDGG
                     SVAPLFTALNINVQTLISELQAILDRLPQVQGGNTQVSQQLVRLLNQSDKLAQQFGDS
                     FISSELFVLASIEDNGDLGKLWKKFGLSKEKLTQAISQIRGGETVNNQNAEETRQALQ
                     KYTIDLTERAKAGKLDPVIGRDEEIRRAVQVLQRRTKNNPVLIGEPGVGKTAIVEGLA
                     QRIVNGEVPEGLKNKRVLSLDMGALIAGAKYRGEFEERLKAVLNELAKEEGQVILFID
                     EIHTMVGAGKTDGAMDAGNLLKPSLARGELHCVGATTLDEYRQYIEKDAALERRFQKV
                     LVDEPTVEDTIAILRGLKERYEIHHHVQITDPAIVAAATLSHRYVSDRQLPDKAIDLI
                     DEAASSLRMEIDSKPEPLDKLERRIIQLKLERQALQKEEDEASRQRLAKLDEEMAAKE
                     REYSELEEIWKAEKSALLGTQHIKSELENARIAMDQARRENDFEKMSELQYGVIPNLE
                     KQLQAVENGEKNADAQLLRTKVTEEEIAEVLSKATGIPVAKMMEGEKEKLLRMEEVLH
                     ARVIGQNEAVDAVTNAIRRSRAGLSDPNKPIGSFLFLGPTGVGKTELSKTLANFLFDD
                     PDAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRHPYSVVLLDEVEKAH
                     PDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSHLIRENAYKGYDVVKEMVM
                     EVVGQHFRPEFINRIDETVMFHSLGQEHIRSIAKIQLQRLIQRLAERGYEVTVTDAAL
                     DHIGKAGFDPLFGARPLKRAIQQELENPLAQQILSGKLLPNKAVTVDYQNGQIVATQ"
     gene            4281..5423
                     /locus_tag="PWX02_001356"
     CDS             4281..5423
                     /locus_tag="PWX02_001356"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDL1729367.1"
                     /translation="MSQLFPEATIRTQAPYRNDIVGSFLRTDALKKAREDFNQQKITR
                     DELREVTRQEVKKLVELQKQHGVKAVSDGEFSRTWWHLDFLAELDGMDWQETEVFNVN
                     FTGHKPKGQTVKIVGDIDFSESHPFVDAYRLLKEAAGDYPTKFTIPAPTMLHIICCVR
                     AEDYQPLPQYQDEQKLYDDIANAYIKAMHKFYDMGLRNLQLDDTSWGQFCAEDKRKEF
                     AARGFNLTDLAEKYVALLNRIVDAKPADMSITMHICRGNFRSSWFSEGGYGPVAEVLF
                     DKCRVDGFFLEYDTERAGTFEPLRHIKDQQVVLGLITSKFGDLEDKNAIIERIKEAAQ
                     IVPINQLCLSPQCGFASTEEGNILTEAAQWAKLNLIKEIAEEVWGS"
     gene            complement(5480..6868)
                     /locus_tag="PWX02_001357"
                     /pseudo
     CDS             complement(5480..6868)
                     /locus_tag="PWX02_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
     gene            complement(7028..7927)
                     /gene="cdd"
                     /locus_tag="PWX02_001358"
     CDS             complement(7028..7927)
                     /gene="cdd"
                     /locus_tag="PWX02_001358"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDL1729368.1"
                     /translation="MFRTDITERFEQLKAEKNCAVTQAIIEQLIKQDFHGQPSYEVVD
                     ELCEKFKRSRVELALYCIAIAACYAVTPVSDFNVGAVAIGKNGDFYFGANQEFSGECM
                     QQSVHAEQSAISHAFLAGETLITDVVVNYTPCGHCRQFMNELNSAASLKVHLPHSQNN
                     LLHSYLPDSFGPKDLGIEKVLFDEQPQQFEVKGDHLQQAAIFAANNAYAPYSNAFSGV
                     ALQVGERIVCGKYTENAAFNPTFLPLQSALNYRLFLGLADIPVTRVVLAEAKGSLSSY
                     AMTQALAKSLFGLEIEYLSLQAV"
     gene            complement(7941..8843)
                     /locus_tag="PWX02_001359"
     CDS             complement(7941..8843)
                     /locus_tag="PWX02_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL1729369.1"
                     /translation="MISSSLKLGYFSLIVATLFWGGNFVLGKVLSGAVQPITLTYLRW
                     LPAFLILVLLFFRPTWRVMHLLKPRLGVMWVLGLLGVILFPATLYEGLKTTTALNASL
                     YLAISPVLVLFLNRLVFKEKISPIILSGAIISLLGVLWLLMQGKLTRLFSLAINQGDL
                     WAIASTISWAVYCCVIRLRPQGISNTVFLTVLVGLAVLTLTPFFIYEYVAESAKNLAN
                     LTALQWSGVGYLVIGPSILSYAFWNFGIAIVGSAKGAVMTNFTPLFAAIFSIILLNES
                     VHSYHIISAILITAGILICSYKKH"
     gene            complement(8840..9196)
                     /gene="folB"
                     /locus_tag="PWX02_001360"
     CDS             complement(8840..9196)
                     /gene="folB"
                     /locus_tag="PWX02_001360"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HDL1729370.1"
                     /translation="MSDKVFIRELTAFASIGAYNWEHTIKQRLVFNIDMAWDFSKAAE
                     TDDVQYCLNYAEVSQKVLDFVESQPFKLVETVAYQLAELLQKEYQLSWIRIELHKPKA
                     VAQASSVGVIVERGHQ"
     gene            9266..9871
                     /gene="plsY"
                     /locus_tag="PWX02_001361"
     CDS             9266..9871
                     /gene="plsY"
                     /locus_tag="PWX02_001361"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDL1729371.1"
                     /translation="MNLIPYLLILTAYLLGSISSAVIFCKLAGLPDPREVGSHNAGAT
                     NVLRIGGKSAALGVLLFDILKGTLPVAMGIYFELSPSQLGFIALAVCLGHIFPIFFQF
                     RGGKGVATAFGAISALSFIVAGGAIGTWLLVFLISGYSSLSAVFTALVVPFYIWWFEP
                     QFTFPVALVCCLLVYRHHDNIQRLWRGQEDKLWDKFKKNRA"
     gene            complement(9945..>11762)
                     /gene="parE"
                     /locus_tag="PWX02_001362"
     CDS             complement(9945..>11762)
                     /gene="parE"
                     /locus_tag="PWX02_001362"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDL1729372.1"
                     /translation="TRPNHLGQEVIDNSVDEALSGFADKIDVILHADNSLEVVDNGRG
                     MPVDIHSTEKISGVELILTKLHAGGKFSNKNYTFSGGLHGVGISVVNALSERVEIQIK
                     RGGEVYSIAFANGVKVEDLTVIGTCPKKQTGTTVRFYPNPKYFDSPRFSVSRLRHLLR
                     AKAVLCPKLTINFIDKINNTEESWYYEDGLSDYLSEALNGYETVPNPPFIGDVTAETE
                     AVSWALTWLPEGGELVAESYVNLIPTAQGGTHVNGLRNGLLKAMVEFCEIHNLLPKGV
                     KLTADDVWNRCAYVLSLKIQEPQFAGQTKERLSSRQASSYVDSTLKDAFSLWLNQNVQ
                     TGKLIAEMAISSAQSRMRAAKKVVRKKLVSGPALPGKLADCTAQDLSRTELFLVEGDS
                     AGGSAKQARDKEYQAILPLRGKILNTWEVSSDQVLASQEVHDIAVALGIDPDSDNLEE
                     LRYGKVCILADADSDGLHIATLLCALFLRHFPKLVQNGHVYVAMPPLYRIDIGKDEVH
                     YALDEAEKEAILARLAKKKGKPNVQRFKGLGEMNPSQLRETTMDPSTRRLVQLTFDDT
                     AEEGELNTFEIMDMLLGKKRAEDRKQWLQNRGDEADLAV"
BASE COUNT         3696 a         2551 c         2307 g         3208 t
ORIGIN      
        1 aaattataat gcttgtttga taaattcagc catttgtgcg tgcggaattg caccaacttg
       61 ctgggcaatc acttcaccat ttttgaataa tagtaaaaat ggaatgctgc gtacgccaaa
      121 ttgtgctggt acagatggat tttcatctac gttcattttg acaattttta ctttaccatc
      181 aaattgtgca tcaacagata aatcgtcaag tacggggccg atcatacggc aaggaccaca
      241 ccaaggcgcc cagaagtcta ataataccgg tacatctgat tttaaaacat cttgttcaaa
      301 tgtagcatct gttgtatgaa tgacattgct cataagttta tccttaaata ttaaattaaa
      361 aaaagaaacg ccctaactgg caagttaagg cgaacagttg tgtataagat agagattaat
      421 ctataaaatt tcaagcaaga tttaccaatt acaccaataa atatttgcat agtgagattt
      481 tattatctct tcaattaaaa gtagaaacgg gctatatcta aacaactgca acaaaaaggg
      541 ctaatcacta agattagccc aactcgttct caacaataat tagaacttca caccgaaacc
      601 ggcaaatagg acaaccggat caagttttac ttttacttcg tgttgcatat cattagttac
      661 gcttggcgtt ttgaatttcg ccgtggtttt gatacgggtg taccacgctg aggcgttgaa
      721 atagatgtta tcggttaatt tcacatcaac accgagattc gccaccggtc cgaaagagtg
      781 ttttttcaca tttaaatccg ttaccgctgc atgtactgat tttgcgtggt aaaaacgggt
      841 atagttcaga cccgcaccga tataaggttt cactttagcg gttggttcaa agaaatagta
      901 ttgagcataa aggctcggtg gtaattgtct taagctcaca acacgaccta aattcgcatt
      961 tgcacctaat gccggcacaa tcgcattgat ttaatgcgag aatggcgttg cacctaataa
     1021 ttcgataccc acattatccg ccaacatata agtggccgtt aaacctaact gggtgttgtt
     1081 ttttactttt aaatcaatat caaccgcagt tttcgtatct gaacttgaat ttgccttaac
     1141 atgcacagca ccggcacgga atatcacatc accagcttga tgtgccatcg cagttccagt
     1201 taataatgcc ccacctaaaa taattgctaa agttgatttt ttcattttca tatcctcaaa
     1261 aataattaga atacctataa tgaggtaaac tttagcagag tgtaaatgaa atgaaataga
     1321 aatgaattga tatttatcaa acttttaaca caaagatacc caacaagagt tattttattt
     1381 atttttgtga tctttttcac atttttactt gaaaattatt taccaagctc catttaatgt
     1441 ttcaacaaga gatgtaaata aaaatttaca ctttctaaac tcattgtaag gagaaagaaa
     1501 tgaattttga aaaaatgacc acaaaactgc aagaagcctt ggcagaatcc caatcgcttg
     1561 cagttggaaa agataatcct tatatcgaac ctgcccattt actgtatgcg ttagtaaaac
     1621 aagatggtgg atctgttgcc cctctcttta ccgcattaaa tatcaatgtg caaaccttaa
     1681 ttagtgaatt gcaagctatt ttagaccgct tgccgcaagt tcaaggcggt aatacacagg
     1741 tttcacagca attagtgaga ttgttaaatc agtcagacaa gctcgcacaa caatttggcg
     1801 acagttttat ttcctctgaa ttgtttgtgt tggcaagcat tgaggataat ggcgatttag
     1861 gtaaattatg gaaaaaattt ggcttaagca aagaaaaact gacacaagct atcagccaaa
     1921 ttcgaggagg agaaaccgtg aataatcaaa acgcagaaga aaccagacaa gcattacaaa
     1981 aatatacgat tgatctgacc gagcgtgcca aagctggcaa gctagatccg gtaatcggtc
     2041 gtgatgaaga aatccgccgt gcggtacaag ttttacaacg ccgcactaaa aataacccag
     2101 tattaatcgg tgaaccgggg gtcggtaaaa ctgcgatcgt agaaggcttg gcacagcgta
     2161 ttgtaaacgg cgaagtgccg gaaggcttga aaaataagcg tgtactttcg ctggatatgg
     2221 gagcattaat tgccggtgcg aaatatcgtg gtgaatttga agagcgttta aaagcggttt
     2281 taaacgaact ggcgaaagaa gaagggcaag ttatcctctt tattgatgaa attcacacga
     2341 tggttggagc aggcaaaaca gacggagcaa tggacgccgg caacttatta aaaccaagcc
     2401 ttgcccgtgg tgaattgcac tgtgtggggg ctacaaccct tgatgaatat cgccaataca
     2461 ttgaaaaaga tgcagccctt gaacgccgtt tccaaaaagt attggtggac gaaccaacgg
     2521 ttgaagatac gattgcgatt ttacgtggtt tgaaagagcg ttatgaaatc catcatcacg
     2581 ttcaaattac agaccctgcc atcgtggcgg cggcaacgct ttcgcaccgt tatgtgtctg
     2641 accgtcaatt gccggataaa gcgattgact tgattgatga agccgcttca agcctgcgta
     2701 tggagattga ttctaaacct gagccgttgg ataaattaga acgccgcatt atccaactca
     2761 aattggaacg ccaagcgtta caaaaagagg aagatgaagc gagccgtcag cgtttagcca
     2821 aattagacga agaaatggca gcgaaagagc gtgaatattc cgagctggaa gaaatctgga
     2881 aagcggaaaa atccgcctta cttggtacac agcatattaa atcggagttg gaaaatgccc
     2941 gaattgcgat ggatcaggct cgccgtgaaa acgatttcga gaagatgtcg gaattgcaat
     3001 atggcgtgat tccaaacttg gaaaaacagt tacaagcggt cgaaaatggc gaaaaaaatg
     3061 cagatgctca gctcctccgt actaaagtca ccgaagagga aattgcggaa gtgctttcaa
     3121 aagcaacggg cattcctgtt gcaaaaatga tggaaggcga gaaggaaaaa ctccttcgta
     3181 tggaagaagt attacacgct cgggttatcg ggcaaaatga agccgttgat gcggtgacaa
     3241 atgcgattcg ccgtagccgt gccggacttt ccgatccgaa taaaccgatt ggttcattcc
     3301 tattcttagg accgaccggc gtgggtaaaa ccgagttaag caaaacctta gctaatttct
     3361 tgtttgacga tcctgatgct atggtgcgaa ttgatatgtc tgaatttatg gaaaaacaca
     3421 gcgtatcccg cttagtgggc gcacctccgg gctatgtcgg ctacgaagaa ggcggctatt
     3481 tgaccgaagc ggttcgccgc catccatatt cggtagtgtt acttgatgaa gtggaaaaag
     3541 ctcaccctga tgtgtttaac attttattgc aagtgttaga tgacgggcgt ttaaccgacg
     3601 ggcaaggcag aacagtcgat ttccgcaata cggtagtgat tatgacctca aacttaggct
     3661 cgcatttaat tcgagaaaat gcgtataaag gctatgatgt ggttaaagaa atggtgatgg
     3721 aagtcgtcgg acagcacttc cgccctgaat ttatcaaccg tattgatgaa acggtgatgt
     3781 tccactcact tggtcaagag catatccgtt cgattgcgaa gattcagcta caacgcttaa
     3841 tccaacgctt ggcagaacga ggctatgagg tgacagtaac cgatgcagca ttagatcata
     3901 tcggcaaggc tggtttcgat ccgctgttcg gagcaagacc actgaaacgt gcgattcaac
     3961 aagagcttga aaatccgctt gctcaacaga tcttaagcgg taaattatta ccgaataaag
     4021 ccgttacggt ggattaccaa aacgggcaga ttgttgcaac acaataatgt ctccgatggt
     4081 gcgagcgtcc tcgctcgtac cttacaagcg gtggatttat cgtaaaaatt tgcaaaaaat
     4141 tgctcgaaaa tgaccgcttg cacagtaaaa attttcatat tgcccttcat taattttcat
     4201 aagctaatga agggcttttt attactcttt ttgctatcat taacggcgtt tataaccaat
     4261 acaattacaa ggagtaattt atgagccaat tatttccgga agcaacaatc cgcactcaag
     4321 ccccgtatcg taatgatatt gtaggcagct ttctacgtac cgatgcgtta aaaaaagcca
     4381 gagaagattt caatcagcaa aaaatcaccc gtgacgaatt gcgtgaagtc acacgccaag
     4441 aggtcaaaaa attagttgag ttgcaaaagc aacacggtgt aaaagcagtc tctgacggtg
     4501 aatttagccg gacttggtgg catttagatt tcttagctga acttgatgga atggattggc
     4561 aagaaaccga agtctttaac gtgaacttta ccggtcataa acccaaaggg caaacggtaa
     4621 aaatcgtggg cgatattgat ttctccgaaa gccacccttt tgtggacgct tatcgtcttt
     4681 taaaagaagc tgccggcgat tacccaacca aattcacgat tccggcaccg acaatgttac
     4741 atattatctg ctgtgtgcgt gcggaagatt accaaccgtt accacaatat caagacgaac
     4801 aaaaactgta cgatgatatt gctaatgctt atatcaaagc aatgcacaaa ttctacgata
     4861 tggggctacg taacttacaa ttagacgaca ccagttgggg acaattctgt gccgaagata
     4921 aacggaaaga gtttgcagcc agaggattta atttaactga tttagcggaa aaatatgtcg
     4981 cattattaaa ccgtattgtt gatgcaaaac cggcggatat gtcaattacg atgcacatct
     5041 gccgtggtaa cttccgttct tcttggttct cggaaggcgg ttatggaccg gttgccgaag
     5101 tattattcga taaatgccgt gttgacggct tcttcttaga atacgatacc gaacgtgcag
     5161 gcactttcga gccactacgc cacattaaag atcaacaagt ggtactaggc ttaattacct
     5221 ctaaatttgg cgatttagaa gataaaaatg cgatcattga gcgtattaaa gaagcggctc
     5281 aaattgtgcc aatcaatcag ctatgcttat caccacaatg cggctttgcc tccaccgaag
     5341 aaggcaatat tttaaccgaa gccgcacaat gggcgaaact aaacttaatt aaagaaattg
     5401 cggaagaagt ttggggtagc taatcctcta taatacaaat agccgagtaa atactcggct
     5461 attttagttt gttttaacat tacaaccaga agagataccc aacagtcgca atcacaaaga
     5521 tagagataat atttagccag aaaccaactt taatcatctc actttgttta atttgccctg
     5581 taccaaatac aatcgcattc ggtggggtgg caaccggcaa cataaaggcg caagatgctc
     5641 ctagaccaat aattaaggct aatccgaccg gtggcatatt taatgcttgt gctatagaga
     5701 tgaaaatcgg cactaacaaa gccgcactgg ctgtgttaga ggtaacctca gttaggaaaa
     5761 taataaatgc tgccacaatt aaaccaatta ggtaaaaatg ccccccttca attagtgaga
     5821 cgaccccatc agccataatt ttgctcgcac cagtttgtcc taatacagca cttaacgtca
     5881 aaccaccacc aaataggaat aatacccccc actcggtgtt ttcttgaatc tgttgccaat
     5941 ttgcgacacg agtaatgcaa ataagtgctg cggcaatcaa tgcaagaatg gtgtcaaagc
     6001 tcgcaatttt gccattaatt cccagaaatg cagaaataat agggttaatt ttatcaccaa
     6061 aaatccaacc aagcgcaata aagacgaaaa tagccaaggt gataatacga gaacgattta
     6121 attcaatttt ctcaaatgat cgatcaaaac taactgaaaa atttggttta aatacgatat
     6181 atagtgttgc aatcattaat gggaataaaa ttagcataat tggcaaacca tattttaacc
     6241 aatcagcaaa agtcagattc aattgagaag caacaatcgc attcggtggg cttcccacga
     6301 aagtacccat tccaccaata ctggcactat aagcaattcc taataacacg aaaagataag
     6361 tattgtggtt ctgtttctga tcaagtttac ttaaaatccc cattgccaaa ggtaacatca
     6421 ttgcagctgt agctgtatta ctcatccaca ttgacaaaaa cgctgttacc gcaaataaaa
     6481 taactgcaat cgataattta cctttagcta atgccataat tttattggca atcatacgat
     6541 ctagctactg aatgtgtaat gctgtcgcta aggcaaagcc tccaaaaaat aagaaaatcg
     6601 ttggatcagc aaaagcaaca agagcttctt ttgatttaac cagccccagt gctacagcca
     6661 aaatcggaat taataatgcc gtaaccgtaa cgtgtaatgc ttctgttagc cataagacgg
     6721 caatgaaaat taacaatgcc aaccccttat tggcttcagg cgtaaaaggc aaggtattta
     6781 acaaaataat aaataaaata gcatctgcaa caaaaatcaa gctattgcgt gtatttttac
     6841 tcggttcaat aagaggagta ttttccataa aaacctcaca aaatatagat aatcagtggt
     6901 atcactatat ataaaaaaac gatataggca aatataattg ttgcaaaagt gtgaagctca
     6961 tcaaaaaatt taacactaaa aaataaattg cttaaagata cagcaccttt gcaaaaataa
     7021 atcaaaatta caccgcttgt agcgaaaggt attcaatctc taacccaaat agtgattttg
     7081 ccaatgcttg agtcattgca taactactga gagagccttt cgcctcagcc agcacaactc
     7141 gagtaacggg aatatcggca agccctaaaa ataatcgata gttcaacgcg ctttgcaacg
     7201 gtaaaaaagt aggattaaaa gctgcattct cagtatattt accacaaaca atacgctcac
     7261 ctacttgcag tgcaacccca ctaaatgcgt tgctgtaagg ggcataagca ttattggcag
     7321 caaaaattgc cgcttgttgt aaatgatcgc ccttaacctc aaattgttgc ggttgttcat
     7381 caaataagac tttctcaatc cctaaatctt tcggtccaaa actatccggc aaatagctat
     7441 ggagcaaatt attttggcta tgcggtaaat gtacttttaa gcttgccgca ctgtttaatt
     7501 cattcataaa ttggcggcaa tgcccacaag gggtatagtt cacaaccaca tctgttatca
     7561 aagtttcccc tgctaaaaat gcgtgcgaga ttgcactttg ctctgcgtga acggattgtt
     7621 gcatacattc cccggaaaac tcttggtttg ccccaaaata gaaatctccg tttttgccaa
     7681 tcgcaaccgc ccctacatta aaatcggaaa ctggtgtaac cgcataacag gcagcaatgg
     7741 caatgcaata taaagccaac tcaacacgag agcgtttaaa tttttcacag agttcatcaa
     7801 ccacttcata agagggctgc ccgtgaaaat cttgtttgat aagttgctca ataatagcct
     7861 gtgtaactgc acagtttttc tctgctttta attgttcaaa acgctctgta atgtctgttc
     7921 gaaacatacg gtacctccgt ttaatgtttt ttatagctgc aaatcagtat ccctgccgta
     7981 atcagtatgg cactaataat atggtaagaa tgaactgatt catttaataa aataatactg
     8041 aatattgctg caaagagcgg cgtaaaatta gtcattaccg ctcctttagc agaaccgact
     8101 attgcaatgc cgaaattcca aaacgcataa gagagaatag aagggccgat aaccaaatag
     8161 cctaccccac tccattgtaa agcggtcaaa tttgctaaat tttttgcaga ttctgctaca
     8221 tattcataga taaaaaatgg cgttaacgtt agaactgcca gccccactaa tacggtcaaa
     8281 aacaccgtat tgcttatacc ttgtggacgt agccgaatca cacaacaata aaccgcccag
     8341 cttatagtag aagcaatcgc ccataaatca ccttgattaa ttgccaagct gaataagcga
     8401 gttagcttcc cttgcatcaa tagccacagc actcccagca aactgattat tgccccactt
     8461 agaataatcg ggctgatttt ctccttaaaa acgagccgat ttaaaaatag caccaataca
     8521 ggcgaaatag ctaaatagag actcgcattc aatgctgttg tcgtttttaa accttcatat
     8581 aaggtagccg ggaataaaat taccccaagc agacctaata cccacatcac acctaaacga
     8641 ggttttaata agtgcattac ccgccaagtt gggcgaaaaa agagcaaaac taaaattaaa
     8701 aaagccggca accaacgcaa ataggttaag gtaataggct gaactgcccc acttaacact
     8761 ttgcctaata caaaattccc accccaaaaa agagtagcaa cgattaaact gaaatacccc
     8821 aatttcagtg atgatgagat cattgatgac ctcgttctac aatcacacct acactgcttg
     8881 cttgggcaac cgctttcggc ttatgcagtt caatccgaat ccaagaaagt tgatactctt
     8941 tttgtaatag ttccgccagt tgataggcaa cagtttcaac taatttgaaa ggttgagatt
     9001 caacaaaatc cagcactttt tgcgatactt ccgcataatt caagcaatat tgtacatcat
     9061 cggtttctgc cgctttggaa aaatcccacg ccatatcaat gttaaaaacc aagcgttgct
     9121 taatcgtatg ctcccaattg taagccccaa tggaagcaaa agcggttagt tctcgaataa
     9181 aaaccttatc actcattttt cgtcctgttt tattttctga atttctgtaa aatatagcac
     9241 aagataaatt taatcaggaa aatttatgaa tttaattccc tatttattga tcttaaccgc
     9301 ctatttgctc ggctcgattt ccagtgcggt cattttttgt aagctggcag gcttgcctga
     9361 cccaagagaa gtcggctcac acaatgccgg tgcaacgaac gtactacgaa ttggcggtaa
     9421 aagtgccgca ttaggtgtgc ttttattcga tattttaaaa ggcacattac cggtggcgat
     9481 ggggatttat tttgaactct ctccctcaca actcggtttc atcgccttag cggtttgtct
     9541 tgggcatatt ttcccgattt tcttccaatt ccgtggcgga aaaggcgttg ccaccgcatt
     9601 tggtgcaatc tccgcattaa gctttattgt tgcaggcggt gctatcggca cttggctact
     9661 ggtgttttta atctccggct attcttcttt aagtgccgtc ttcactgcct tggtcgtacc
     9721 gttttacatt tggtggttcg aaccacaatt tactttcccc gtcgccttag tttgttgcct
     9781 attggtttac cgccatcacg ataatattca acgcttatgg cgtgggcagg aagacaaact
     9841 gtgggataaa ttcaagaaaa atagagcata aaaataggca tagtgaaccg ttcgctatgc
     9901 ctatttgcaa attattgcgg aaaaatcacc gcttgtcttc tcgactaaac cgctaaatca
     9961 gcctcatcgc ctcgattttg tagccattgt ttacgatctt ccgctcgttt tttacccaat
    10021 aacatatcca taatttcaaa ggtattcaat tcaccctctt ctgccgtatc atcaaaggtt
    10081 aattgcacta agcggcgagt gcttggatcc atcgtcgttt cacgcaactg actcggattc
    10141 atttcgccta agcctttaaa gcgttgcaca tttggcttgc cttttttctt ggcaaggcga
    10201 gctaaaattg cctctttttc cgcctcatct aaagcataat gcacctcatc tttacctata
    10261 tcaatacgat aaagtggcgg cattgccaca taaacgtgtc cgttttgcac caatttcggg
    10321 aaatgacgaa ggaataacgc acacagcaag gtagcaatat gcaaaccgtc agaatccgca
    10381 tcggcaagaa tacaaacttt gccataacgc aactcttcta aattatcgct atcagggtca
    10441 attcccaagg ctaccgcaat atcgtgtact tcttgtgaag ccaatacttg gtctgatgaa
    10501 acttcccaag tgtttaaaat cttaccacgc agcggcaaaa tggcttgata ctctttatct
    10561 cgagcttgtt ttgccgaacc gccggcagaa tcaccttcta ccaagaaaag ttcggtacgg
    10621 ctgagatctt gagcagtaca atccgccagt ttgcccggta aagccggacc actcaccaat
    10681 ttcttacgca ccactttttt cgccgcacgc atacggctct gggcggaact aattgccatt
    10741 tcagcaatta atttacctgt ctgcacattt tggttaagcc ataaactaaa ggcatctttc
    10801 aaggtgctat ccacatagct tgaggcttga cgggaagaca aacgctcttt ggtctgccct
    10861 gcaaattgtg gctcttgaat tttcagagaa agcacataag cacaacggtt ccacacatca
    10921 tcagcggtta attttacccc tttcggcaat aaattatgga tttcgcaaaa ctccaccatc
    10981 gcctttaata aaccgttacg caagccatta acgtgcgtgc cgccttgtgc ggtcggaatc
    11041 aggttcacat agctttccgc tactaactcg cccccttccg gcaaccaagt taatgcccag
    11101 cttaccgcct ctgtttcagc agtaacatca ccaataaacg gtggattcgg cacggtttca
    11161 taaccgttta atgcctcgct caaataatca gataagccgt cttcgtaata ccaactttct
    11221 tcggtattgt tgattttatc aataaaattg atggtgagtt tcgggcaaag caccgctttg
    11281 gctcgtaaca aatggcgtaa acgactcacc gaaaaacgag ggctatcaaa atattttggg
    11341 ttcgggtaaa aacgcactgt cgtgccggtc tgttttttcg ggcaagtacc aattaccgtc
    11401 agatcttcca cttttacccc gttggcaaag gcaatcgaat agacttctcc gccacgttta
    11461 atctgaattt caacccgttc ggacagggca tttaccaccg aaatccccac cccgtgcaaa
    11521 ccaccggaaa acgtatagtt tttattggaa aatttaccgc cggcgtgcaa tttcgttaag
    11581 ataagctcca cccctgaaat tttctcggtg gaatgaatat cgaccggcat tccccgcccg
    11641 ttatccacca cttccaacga attatcagca tgaagaatca catcaatttt atctgcaaag
    11701 ccggacaacg cctcatcgac actattatca atcacctcct gccccaaatg gtttggacgt
    11761 gt
//
LOCUS       DANJWC010000071        11727 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.071, whole genome shotgun sequence.
ACCESSION   DANJWC010000071 DANJWC010000000
VERSION     DANJWC010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11727
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.071"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            11..730
                     /gene="fdhD"
                     /locus_tag="PWX02_001363"
     CDS             11..730
                     /gene="fdhD"
                     /locus_tag="PWX02_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012621653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDL1729373.1"
                     /translation="MPVALVYNGISHTVMMATPANLEDFALGFSLAEGILNAPSELYG
                     LDVIEQENGVEIQLEISSRQFVALKDKRRSMAGRTGCGICGVEQLEQVNQSCKIVKKN
                     NRLQNVNPLILEDCLLQLENAQFLSKETGASHAAAFFTPDGKLLAIREDVGRHVALDK
                     LIGWYAKADFPIGFVFVTSRASFEMVQKCLAVGIEMLCAISATTEMAVNMARENQFTL
                     LAFARKGKATIYAGEERLALN"
     gene            733..1660
                     /locus_tag="PWX02_001364"
                     /pseudo
     CDS             733..1660
                     /locus_tag="PWX02_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
     gene            complement(1752..2261)
                     /locus_tag="PWX02_001365"
     CDS             complement(1752..2261)
                     /locus_tag="PWX02_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL1729374.1"
                     /translation="MITLHTNFGDIKIELNFEKAPITAKNFEDYAKEGFYNGTIFHRV
                     IDGFMIQGGGMTAGMAEKPTKDPIQNEANNGLSNKRGTIAMARTSDPHSASSQFFINV
                     ADNTFLDYRSKEMFGRSVVQDWGYAVFGEVVEGMDVVDKIKGVKTGNKGFHQDVPVED
                     VVIESVSVA"
     gene            2417..3796
                     /gene="cysS"
                     /locus_tag="PWX02_001366"
     CDS             2417..3796
                     /gene="cysS"
                     /locus_tag="PWX02_001366"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDL1729375.1"
                     /translation="MLKIYNTLKREKEEFKPINPNQVGMYVCGVTVYDLCHFGHGRTF
                     VSFDVIARYLRYSGYNLRYVRNITDVDDKIIKRALENNETCDELVDRMIAEMHKDFDA
                     LNILRPDVEPRATKHIPEIIAMVEKLIHNGHAYVAADGDVMFDVESFQKYGALSRQNL
                     EQLQAGARVEIKSIKKNPMDFVLWKMSKAGEPSWESPWGNGRPGWHIECSAMNSKELG
                     EHFDIHGGGSDLMFPHHENEIAQSCCAHGGDYVNYWLHTGMLTIDKEKMSKSLGNFFS
                     IRHMLGLYDAESLRYFFLTAHYRSLLDYSIDNLDLARSALERLYTALRGCDVSGEAIG
                     GEHYVEAFKTAMDDDFNTPGALAVLFEIARELNKLKTEDMAKANGLAVRLKELAGVLG
                     LLEQDPDAFLKGDANDDETAEIEALIKQRNEAKAAKNWAVADEVRDKLKAINIVLEDT
                     PNGTIWRKA"
     gene            complement(3911..4858)
                     /gene="thrB"
                     /locus_tag="PWX02_001367"
     CDS             complement(3911..4858)
                     /gene="thrB"
                     /locus_tag="PWX02_001367"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDL1729376.1"
                     /translation="MALRIYAPASSANISVGFDSLGAAISPIDGSLLGDVVQIEESEQ
                     QFELESAGYFVRKLPKEPQKNIVYQAYVLFSERLKLRGGNVKNLRLTLEKNMPIGSGL
                     GSSACSIVAALVALNKFHDEQFSKMELLEMMGELEGRISGSIHYDNVAPCYLGGLQLM
                     TQSLGNICQPLPFFDEWYWVLAYPGVEVSTAEARAILPKSYTRQDMIQQARYLGSFVH
                     ACHTHQDILAATMMKDLIAEPYREDLLPNFPAVRQGCKDLGALAVGISGSGPTMFAIA
                     PDLEHAQRLVSYLEKEYLQNNEGFIHICKVDNQGARELK"
     gene            complement(4858..7314)
                     /gene="thrA"
                     /locus_tag="PWX02_001368"
     CDS             complement(4858..7314)
                     /gene="thrA"
                     /locus_tag="PWX02_001368"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HDL1729377.1"
                     /translation="MRVLKFGGTSLANPERFMQAAEIIEKAHLADQAAGVLSAPAKIT
                     NHLVAIVDKAIAGESFQSNLSEATEIFHNIINGLYATNNHFAKEALLALVEAELNQIQ
                     QVAAEAAQTKSLSDKIAATIHCRGEKLSIAMMQAWFEAKGYEVTRIDPVEKLLAHGSY
                     LESSVDIAESTKRVDAGSIPKKNIVLMAGFTAGNEQGELVLLGRNGSDYSAACLAACL
                     KADVCEIWTDVDGVYTCDPRLVPDAVCLETMSYQEAMELSYFGAKVIHPRTIGPLVPL
                     NIPCLIKNTGNPDGKGTLIDGNVANDNLKVKGITNLDNVAMFNVSGSGMQGMVGMAAR
                     VFSTMSKAGISVILITQSSSEYSISFCVPAKSAEKALAALNTEFAQELSENLLDPIDI
                     IKDLSIVSVVGDGMRTAKGIAARFFSALAQANISIVAIAQGSSERSISAVVPLNKAIE
                     AVKATHKSLFNSKRVVDMFLVGVGGVGSELIEQVKQQKEFLAKKDIEIRVCALANSDK
                     MLLNENGLNLDNWKEDLEKAIQPSDFDVLLSFIKFHHVVNPVFVDCTSAQSVANLYAR
                     ALKEGFHVVTPNKKANTSGYDYYLEMRENARQSQHKFLYETNVGAGLPVIENLQNLLA
                     AGDEVEKFEGILSGSLSFIFGKLEEGLTLSQATLIAKEKGFTEPDPRDDLSGADVARK
                     LLILVRETGLPLEFEDIEIEGVLPKGFSEGMSRDEFLQALPSLDEEFSKRVEAAKAEG
                     KVLRYVGSITGDKCRVAIEAVDENHPLYKVKDGENALAFLTRYYNPIPLLLRGYGAGN
                     SVTAAGIFADILRTLQGGVN"
     gene            7758..8399
                     /gene="sodA"
                     /locus_tag="PWX02_001369"
     CDS             7758..8399
                     /gene="sodA"
                     /locus_tag="PWX02_001369"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005708524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HDL1729378.1"
                     /translation="MAYTLPELGYAYDALEPHFDAKTMEIHHSKHHQAYINNANAALE
                     AHPELLEKCPGALIKDLSQVPAEKRIAVRNNVGGHVNHTLFWKGLKTGTTLQGALKEA
                     IERDFGSVEAFQSEFEKAATTRVGSGWAWLVLEEGKLAVVSTANQDNPLMGKEVAGVS
                     GYPILVLDVWEHAYYLNYQNRRPDFIKAFWNVVNWDEAARRFEEKVATCGCAK"
     gene            complement(8462..9586)
                     /gene="alr"
                     /locus_tag="PWX02_001370"
     CDS             complement(8462..9586)
                     /gene="alr"
                     /locus_tag="PWX02_001370"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDL1729379.1"
                     /translation="MKPATATIDSQALRHNIRLIKSFAPQQKLLAMIKANAYGQGLLP
                     AAGTLADMVDGFGVARLREALEIQETGYTGKIVLVEGFFDREELLKTLSRKFDTVIHN
                     LEQLELLEQVAKEWQAEQQKGFWTRKAKIYFPINIWLKIDTGMHRLGIHPEQVDHFVE
                     RLKNCALVNSISFVSHFSRADELDCGYTEKQIAVFEQATQKYQDHDRSISASSGILYW
                     KQAHYDWVRPGIIMHGISPHYTPITDLGFKPVMTLSSSLIAVRTHKAGEPVGYGGAWV
                     SPKDTKLGVIAMGYGDGYPRNAPEGTPVLINGRIVPIVGRVSMDMLTVDLGAESQDKV
                     GDKAIFWGEQLLIEDVAKHIGVISYELITKLTPRVIFEYK"
     gene            9716..11677
                     /gene="recD"
                     /locus_tag="PWX02_001371"
     CDS             9716..11677
                     /gene="recD"
                     /locus_tag="PWX02_001371"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HDL1729380.1"
                     /translation="MLNLLLELKQEKAISDLNYQFARFIDNKQKPYGYSPLEQDLAVL
                     AAALVSYQVGLGHTALRLENLSLSSLFELNSKPQTYHLLEQILQKIEQIPPLEWQSVL
                     KNHIAFSYSPETIKPMLFQNSLLYFYRYWQAEHRIAGYLQQAVDFSEKFANTELNQSI
                     LNQLFTGKAQTIDWQKIAVATALEKQFSLISGGPGTGKTTTVVKLLLGLQLKQQALQK
                     PYLQIALAAPTGKAAARMKESLESKLAEEKGLSEELKRSIPTEAVTIHRLLGARPLTE
                     KTKYHTKNPWHYDLVVLDEASMIDLSMMEKVVQALKPNSRLIMLGDKDQLASVEAGSI
                     MGELGSFIDNGYSQSHCDYLKAVTDYQIEAGNALPIGDCLSHLKHSYRFGEKAWIGEL
                     ANAVNDQKINLSWEIFDKYKNSDKLENFCYPETKETTDKSSWIEKSVQMVVDKAVELY
                     RDYLQAVQKREKNPDSVSVKAIFEKFQKVRILSALRVSELGVERLNMAIAEGLRKASL
                     VKFNHSRDRYIGKPILMTENMPINSIASGDVGIILPDENGESRVYFDSLQENREHHSL
                     PLGRVSNYEAAYIMTVHKSQGSEFEHTVLVLPHSISPVLTKELIYTAITRAKDKFTLF
                     GSEKVWKYSVGAKTERQSGLKEQIRGRFS"
BASE COUNT         3419 a         2407 c         2511 g         3390 t
ORIGIN      
        1 aatttcagaa gtgccggttg ctttggtata taacggtatt tctcatactg taatgatggc
       61 aacaccggct aatttggagg acttcgcttt aggattctca ttggcggaag ggattttaaa
      121 tgccccatcg gaattatatg gtttagatgt aattgaacaa gaaaatggag tagaaattca
      181 gcttgaaata tcgagccgcc aatttgttgc attaaaagat aaacgccgct caatggcagg
      241 cagaacaggt tgcggtattt gcggtgtaga gcaattagag caggttaatc aaagttgcaa
      301 aattgttaag aaaaataacc gcttgcagaa cgtcaatcca ctgattttag aggattgttt
      361 attacagcta gaaaatgctc aattcttatc gaaagaaacc ggtgcaagcc acgccgctgc
      421 attttttacc ccggatggaa aactgcttgc cattcgtgaa gatgtgggca gacacgtggc
      481 gttggataaa ttaatcggtt ggtatgctaa agctgatttt ccgattggat ttgtgtttgt
      541 aaccagtcgt gccagctttg aaatggtgca gaaatgctta gccgtcggca ttgaaatgtt
      601 gtgtgccatc tcagcgacca ctgaaatggc agtgaatatg gctcgtgaaa atcaatttac
      661 cttacttgct tttgcccgca aaggcaaagc gaccatttat gcaggagaag agagactcgc
      721 tcttaattga taatggaaat agccctttta ttagccgata aaattctaca attaaccctg
      781 atggtgctgc tcggctacct catcgttaaa accaaattac tcaaatcgga gcacagttat
      841 ccgatttcgg ttatcggact acattatcag cccggcaatg ctgattactg cttttcaagt
      901 agattacacc accgaaatac taaacggctt gtatctggca tttttaatct ccgtcttatt
      961 aaacggcttg ttaattctgc ttggttggct gctcaaaaga gcgttgaaat tagacaacat
     1021 agaacaagcc accgctattt attccaactc cggcaactta attattccga ttgttgcctc
     1081 cctgttcggc aatgaatggg tgatttattc aacggctttt attgtggtgc aaaatgtgtt
     1141 gttttggtct cacttacggc tgctgatttg tggcaaaggc aatgtgccgt ttagaaagat
     1201 gatctttaat atcaacatta ttgcgattgt gattggtatg atgttgtttt tgctacatat
     1261 caaactgccc gctacgcttg caaacacgct ctcaacttta gggcatatgt tagggccttt
     1321 agcaatgctt gtggcaggga tgttaattgc agcaattcca tttaaagaga ttattaccga
     1381 taaacgcatt tacttggtgg cttttctccg cttgattttt attccgctga ttttgttagc
     1441 ggtgattaaa ctcggcggtt ttgcgagctg ggtagagaat ggaaaaacgg tggtaatgat
     1501 tacattctta gccaccatca gcccttctgc tgcaacggcg acccaaatgg cggtggctta
     1561 cgggcaagat gccaaaaaag ccagtgcgat ttatggcgta accactttac tttgtgtgtt
     1621 tacaatgccg ctgattattt ggctgtatca atttctataa aaacaagcgg tcgatttctg
     1681 ctactttttt gcaacgctga gaaatgcaaa aaattgtgaa aaatcaaccg cttgtaggct
     1741 aatttgcgga attaggcaac gcttacagac tcaatcacca catcttcaac cggcacgtct
     1801 tggtggaagc ctttattgcc ggtttttacg cctttgattt tatcgaccac gtccatacct
     1861 tcaaccactt cgccaaatac tgcatagccc caatcttgca ctacacttct gccgaacatc
     1921 tcttttgagc gataatctaa gaaagtatta tccgccacat taatgaaaaa ctgtgacgaa
     1981 gcagaatgcg gatcagaagt gcgagccatt gcgattgtgc cacgcttgtt gcttaaaccg
     2041 ttgttcgctt cgttttgaat cggatctttt gtcggttttt cagccatacc tgccgtcatt
     2101 ccgccacctt gaatcataaa gccgtcaatc acacggtgga aaatcgtgcc gttataaaac
     2161 ccttctttag cgtaatcttc aaagttttta gcggtaatcg gggctttttc aaagtttaat
     2221 tcgattttaa tgtcaccgaa attggtatgt aatgtaatca ttctttgttc ctttataaat
     2281 aaaaatggaa tggaaaataa atttcgctcg ctattattaa aaagtttacg ccaaattgca
     2341 aaaaaatctc aaaatcagac cgcttattcg ctatacttag ccccatttgt tactgaatgt
     2401 tacacaagag gactttatgc tcaaaattta taacacctta aaacgtgaaa aagaagaatt
     2461 taaaccgatt aacccaaatc aggtgggaat gtacgtctgt ggcgtcacgg tttacgatct
     2521 ttgtcatttt gggcacggtc gcacttttgt ctctttcgat gtgattgccc gttatttacg
     2581 ttattcaggc tacaatttac gctatgtgcg taacattacc gatgtggacg acaaaatcat
     2641 caaacgtgcg ttagaaaata acgaaacttg cgatgaatta gtggatagaa tgattgctga
     2701 aatgcacaaa gattttgatg cgttaaacat tcttcgcccg gacgttgagc cacgagccac
     2761 caagcatatt cctgaaatta ttgcgatggt agaaaaactg attcacaacg gacacgctta
     2821 tgtcgcagcc gatggcgatg tgatgtttga tgtggaatcg ttccagaaat atggtgcatt
     2881 atcccgccaa aatttagagc aactacaagc cggtgcaagg gttgaaatta aatccatcaa
     2941 gaaaaacccg atggattttg tcttgtggaa aatgtcgaaa gccggtgagc caagctggga
     3001 aagcccgtgg ggcaatggca gaccgggctg gcatattgaa tgttcggcaa tgaatagcaa
     3061 agagttgggc gaacattttg atattcacgg cggtggctcg gatttaatgt tcccgcatca
     3121 cgaaaacgaa attgcacaat cttgctgtgc tcacggtggc gattatgtca attattggtt
     3181 acacactgga atgctcacca ttgataaaga aaaaatgtcg aaatctttgg gcaatttctt
     3241 cagcattcgc catatgttag gcttatacga tgccgaatcg ttacgttatt tcttcttaac
     3301 cgctcattac cgttcgttat tagattacag cattgacaac cttgatttag cccgttcggc
     3361 tcttgagcgt ttatataccg ctctgcgtgg ttgtgatgtg tcgggcgaag ccattggcgg
     3421 agaacactat gtagaagcct ttaaaaccgc aatggacgat gatttcaaca ccccgggggc
     3481 attagcggtg ttatttgaaa ttgcccgtga gttgaacaaa ctcaaaaccg aagatatggc
     3541 aaaagccaac ggtttagccg ttcgtttaaa agagttagcc ggcgtgctag gcttactgga
     3601 acaagacccc gatgcgtttt taaaaggtga tgcgaatgac gatgaaacgg cagaaatcga
     3661 agccttaatc aaacaacgca acgaagccaa agcggctaaa aactgggcgg ttgccgatga
     3721 agtgcgagat aagctcaaag cgataaatat tgtcctcgaa gacaccccaa acggcacaat
     3781 ttggcgtaag gcatagcctt cgattgttta taagtgatag acttttcaaa aaattaatta
     3841 aaaagggctg ctccaagtgg tattggagtc agtcctttaa gttatttagt gatatggttg
     3901 ttcgagaggg ctatttcagc tctcttgctc cttgattatc gactttacag atatgaataa
     3961 agccttcgtt gttttgcaga tattctttct ctaaatagct cactaaacgt tgagcgtgtt
     4021 cgagatcagg ggctatcgca aacattgtcg gaccggaacc tgaaatacca accgctaacg
     4081 cacctaagtc tttacagcct tgacgaacag ccggaaagtt cggcagtaaa tcttcacggt
     4141 aaggctcagc aattaggtct ttcatcattg tagcagccaa gatatcttga tgtgtatggc
     4201 aagcatggac gaaagatcct aagtagcggg cttgctgaat catatcttga cgagtatagc
     4261 ttttcggcaa aatagcacgg gcttcggcgg ttgatacttc cacgcccggg taggcaagca
     4321 cccaatacca ttcatcaaag aatggcagag gttggcaaat attgccgagc gattgtgtca
     4381 ttaattgcag gccacctaaa tagcaagggg caacgttgtc gtagtggatt gagcctgaaa
     4441 ttctgccttc caattcgccc atcatttcca gtaattccat tttggagaac tgttcatcgt
     4501 ggaatttatt gagtgccact aatgctgcaa caatcgagca ggcacttgag ccaagccctg
     4561 aaccaatcgg catatttttt tcgagggtta aacgcaaatt ttttacgttg ccaccacgca
     4621 gttttaagcg ttcactgaac agcacatagg cttggtaaac aatgttcttt tgtggttctt
     4681 ttggcaattt acgcacaaaa taacctgcac tttctagctc aaattgttgt tcactctctt
     4741 caatttgaac tacatcgccg agcaaagagc cgtcaatcgg ggagattgca gcgcctaatg
     4801 agtcaaaacc cacactaata tttgcacttg aagctggggc ataaattcgt aacgccatta
     4861 gttcactcct ccttgtaaag tacgcagaat atctgcaaag ataccggcag cggttacgct
     4921 attgcccgca ccatacccac gcaataataa tggaataggg ttgtagtaac gtgtgagaaa
     4981 agcaagggca ttttcgccgt ctttaacttt gtaaagtggg tggttttcgt ccacggcttc
     5041 aatggcaact cggcatttat cacctgtgat cgagcccaca taacgcaaca ctttgccttc
     5101 agccttggca gcttctacac gcttgctgaa ttcttcgtct aaagacggta gagcttgtaa
     5161 aaactcatca cgactcattc cttccgagaa gccttttggc aatacgcctt caatttcaat
     5221 atcttcaaat tcaagcggta agccggtttc acgaacaaga attaagagtt tacgagcaac
     5281 atctgcaccg gataaatcat ctcgtggatc tggctcggta aagccttttt ctttggcgat
     5341 taaagttgct tgcgaaaggg ttaaaccttc ttctaatttg ccgaaaatga aagagagcga
     5401 accggataaa atcccttcaa atttctctac ttcgtcaccg gcagctaata ggttttgtaa
     5461 gttttcaatt accggtaaac ctgcccctac attagtttcg tagaggaatt tgtgctggct
     5521 ttggcgggcg ttttcacgca tttctaagta gtaatcgtaa cctgatgtat tggctttttt
     5581 gtttggtgtt accacgtgga agccctcttt taaggctcgt gcgtacaggt tcgccaccga
     5641 ttgtgccgaa gtgcaatcta caaatactgg gtttaccacg tggtggaatt tgatgaaaga
     5701 aagcaataca tcaaagtctg acggctgaat tgctttttct aaatcttctt tccagttgtc
     5761 taaatttaag ccgttttcgt tgagtaacat tttatcagaa ttagctaaag cacacacacg
     5821 gatctcaata tccttctttg ccaaaaactc cttttgttgt ttgacttgct cgatgagttc
     5881 gctacccaca ccgcctacac ccactaaaaa catatccacc acacgtttac tgttaaatag
     5941 cgatttgtga gttgctttta ctgcttcaat cgctttgttt aacggcacga cagcagaaat
     6001 ggaacgctca gatgaaccct gtgcaattgc cacaatactg atgttggctt gggctagggc
     6061 agagaagaag cgagccgcaa ttcctttggc ggtacgcata ccatcaccca ccaccgaaac
     6121 aatggataaa tctttaataa tatcaatcgg gtcaagtaag ttttcagata actcttgtgc
     6181 aaattcagta tttagagccg ctaacgcttt ttctgccgat tttgccggca cgcagaagct
     6241 gatgctatat tctgatgatg attgggtaat taaaatcacc gaaataccgg cttttgacat
     6301 cgtcgagaac acacgggcag ccatgcccac cattccctgc attcctgaac ctgaaacgtt
     6361 gaacatcgcc acgttatcaa ggttggtaat cccttttact ttgagattat cgttcgccac
     6421 attgccatca attaatgtgc cttttccgtc cggattgccg gtatttttga ttaaacaagg
     6481 aatattgagc ggcactaatg gcccgatggt gcgagggtgg atcactttcg ccccaaagta
     6541 ggaaagctcc attgcctctt gatagctcat tgtttctaag catactgcat caggcaccaa
     6601 acgagggtcg caggtgtaaa cgccatctac gtccgtccag atttcacaaa catcggcttt
     6661 taaacacgct gccaaacaag cggcagaata atctgaaccg ttacgcccta ataacaccag
     6721 ttcgccttgc tcattacctg cggtaaaacc agccattagc acgatatttt tctttggaat
     6781 tgaacctgca tcaactcgct tggtggattc ggcaatatcg accgaagatt ccaaatagct
     6841 gccgtgagcc aataattttt caacagggtc gatacgagtg acttcatagc cttttgcctc
     6901 gaaccacgct tgcatcatcg caatagagag tttttcacca cggcagtgaa tagtggcagc
     6961 aattttatct gacaacgatt tagtttgagc tgcttctgct gccacttgct gaatttggtt
     7021 taattccgct tccactaagg ctaacagtgc ttctttagca aagtgattat ttgtagcata
     7081 taagccgtta atgatgttgt ggaaaatctc ggtagcttcg gaaaggttag attgaaatga
     7141 ttcaccggca atcgctttat ctacaatagc gacaagatga ttggtaattt tggctggggc
     7201 ggataatacg ccggcagctt gatctgccaa atgagctttc tcgataatct ctgccgcttg
     7261 cataaagcgt tcagggttgg caagagaagt gcctccaaat ttaagaactc gcatagttac
     7321 tcctgataaa ttatttgtaa atttttatac aaaaaaaccc gcttgtagcg ggttttcggt
     7381 ttattctttc attatttcag ccaattaaaa cccgcactat gttggtgaca taatggtggt
     7441 aataatcgtt gtcatcatat taaaacaatt cattggcgga atctcggtca agagagtgaa
     7501 taatggttca agaatgcgtg aaaataatgc ctttgtcaat gggcttaacg ctgtttttca
     7561 ccacattttt ttaaatttct ttgtctttct atataaaaca attaaaagca agaatttgat
     7621 taggaacctt tctaaattag atagtgaaat aattttgttc tagatcaaaa aaatgtgcat
     7681 tttttgtgtg tttcttgata aaatgtactc ggagtttaac tctgattaac ctatttacta
     7741 tttgagagga attaactatg gcatatacat taccagaatt aggctacgct tatgatgcgt
     7801 tagagccaca tttcgatgct aaaacaatgg aaatccacca ttctaaacat caccaagctt
     7861 acatcaacaa tgcaaatgca gcattagaag ctcaccctga attattagaa aaatgtccgg
     7921 gtgcattaat caaagatttg agccaagtgc ctgcagaaaa acgtatcgca gtgcgtaaca
     7981 acgtaggtgg tcatgtaaac cacactttat tctggaaagg tttaaaaaca ggcactacct
     8041 tacaaggtgc attaaaagaa gcgattgagc gtgatttcgg ttcagttgaa gctttccaat
     8101 cagaatttga aaaagcagcg actactcgtg ttggttcagg ctgggcgtgg ttagtattag
     8161 aagagggtaa attagccgtt gtttcaaccg ctaaccagga caacccacta atgggtaaag
     8221 aagtggcagg cgtttcaggc tatccgattt tagtcttaga cgtttgggaa cacgcttact
     8281 acttaaacta ccaaaaccgc cgtccggatt tcattaaagc attctggaac gttgtaaact
     8341 gggacgaagc cgctcgccgt tttgaagaaa aagtagcaac ctgcggttgt gcgaaataat
     8401 attttcgatc ttgaataaga tacaagcggt aaaatttgtt gaaaatttta ccgcttgttg
     8461 tctatttata ttcaaaaatc acacgaggag tgagtttagt aatcagctca tagctaatca
     8521 cgccaatgtg tttcgccaca tcttcaatta atagttgctc tccccaaaaa atggctttat
     8581 cgcccacttt atcttggctc tctgcaccta aatcgaccgt cagcatatcc atcgaaactc
     8641 gaccgacaat cggcactatt ctaccgttaa tcaacacagg ggtaccttca ggagcattgc
     8701 gagggtagcc atcgccgtag cccattgcaa tcacgccgag tttggtatct ttagggctta
     8761 cccaagcacc accatagcct acaggctcgc cggctttatg ggtacgcaca gcgatcaggg
     8821 aagaggagag cgtcattacc ggcttaaaac ctaaatcggt aatcggcgta tagtggggag
     8881 aaatgccgtg cataataata cccgggcgaa cccaatcata atgagcctgt ttccaataaa
     8941 gaataccgct tgaggcggaa atactgcggt cgtgatcctg atatttttgc gtagcttgct
     9001 caaacaccgc aatttgtttt tcggtatagc cacaatcgag ttcatcggca cggctaaaat
     9061 gactcacaaa gctgattgaa ttgaccaagg cacaattttt caaacgttca acaaagtgat
     9121 cgacttgctc cgggtgaatg cctaagcggt gcattccggt gtcgattttt agccaaatgt
     9181 taatcgggaa gtagattttc gctttacgag tccaaaagcc tttttgttgc tctgcctgcc
     9241 attcttttgc tacttgctcg agtaattcca gttgttcgag gttatgaatg acggtatcaa
     9301 acttgcggga aagggttttg agtagctctt ctcggtcaaa aaaaccttca actaacacaa
     9361 ttttgcctgt gtatccggtt tcttgaattt ctaaggcttc acgcaaacgg gcaactccaa
     9421 agccatcaac catatctgct aatgtgccgg cagccggcaa taagccttgc ccgtaagcat
     9481 tggctttgat catcgccaat aatttttgct gtggagcgaa agatttgatc aagcgaatat
     9541 tatggcggag ggcttgacta tcaatggttg ctgttgctgg tttcatactt atctacatag
     9601 ttaaaatcac aagaaaggct aaattatacg cctaaaataa aaaatccctt agaaaataat
     9661 gcaaaaaaga ttagtattat cacttaatgt taggtggtcg atttaaggtt tgattatgct
     9721 gaatttatta ttggaactta aacaagaaaa ggcgatttct gacttaaatt atcaatttgc
     9781 ccgttttatt gataataagc agaaacccta tgggtattcg cctttggagc aagatttagc
     9841 tgtgttagcc gctgcgttgg tttcttacca agtgggactt gggcatacgg cattacgttt
     9901 ggaaaatctc agtctttctt ctctttttga attaaatagc aagccacaaa cttatcattt
     9961 gctcgaacaa attttgcaaa aaattgagca aattccaccg cttgaatggc agagtgtgct
    10021 gaaaaatcat atcgctttta gctattctcc cgaaacaatt aaaccgatgt tattccaaaa
    10081 cagcttgctc tatttttatc gctactggca agcggagcat cgcattgccg gttatttaca
    10141 acaagcggtt gatttttcgg aaaaatttgc aaataccgaa ttaaaccaaa gcattctcaa
    10201 ccaactattc actgggaaag ctcaaactat cgactggcaa aagattgccg ttgcaacagc
    10261 attagaaaag cagtttagct taatttcagg tgggccggga accggaaaaa caactacggt
    10321 agtaaaatta ttactcgggc tgcaattaaa acagcaagca ctacagaaac catatttgca
    10381 aatcgctctg gctgcaccaa ccggaaaagc agcagcaaga atgaaagagt cgctggaaag
    10441 caagttggcg gaagaaaagg ggttatctga ggagttaaaa cgctctattc caactgaagc
    10501 agtgactatt catcgcttgc ttggtgcgag accattaact gagaaaacca aatatcatac
    10561 caaaaatccg tggcactatg atttggtcgt attagatgag gcttcaatga ttgatttatc
    10621 aatgatggag aaagttgttc aagcgctcaa accaaactct cgcttgataa tgctgggaga
    10681 taaagatcag cttgcttctg tggaagctgg ttcaattatg ggggaacttg gctcttttat
    10741 tgataatggt tatagccaat cccattgcga ttacttaaaa gccgttaccg actaccaaat
    10801 tgaagccggt aatgcgttgc cgattggtga ttgtttatct cacttaaaac atagctatcg
    10861 atttggcgaa aaagcctgga ttggagagct tgcaaatgca gtaaatgatc aaaaaattaa
    10921 cctttcgtgg gagattttcg acaaatataa aaacagtgat aagttagaaa atttctgtta
    10981 tccggaaacg aaagagacaa cggataaatc aagctggatt gaaaaaagtg tgcaaatggt
    11041 ggtggataaa gcggttgaac tttatcgtga ttatttacaa gcggttcaaa aacgggagaa
    11101 aaatccagat tcagtgagtg tgaaagcgat ttttgagaaa tttcaaaaag tccgaatttt
    11161 atcagcctta agggtaagtg aactgggggt tgaacgtctt aatatggcga ttgcggaagg
    11221 cttgcggaaa gctagcttag tgaaatttaa tcatagccga gaccgctata tcggaaaacc
    11281 gattttaatg acagaaaata tgccgataaa tagcatagcc agcggtgatg tcggcatcat
    11341 tttgccggac gaaaatggag aaagccgagt ttattttgat tccttacagg aaaatagaga
    11401 acaccatagt ttgcctctag gtagggtttc aaattatgaa gcagcttata ttatgactgt
    11461 gcataaatcg caaggttctg aatttgaaca tacggtattg gtgttgcccc attcgatttc
    11521 cccggtgctg acgaaagagt tgatttacac cgcaatcaca agggcaaaag ataaatttac
    11581 cttatttggt agcgaaaaag tttggaaata cagtgttggt gccaaaaccg aaagacaaag
    11641 tgggctaaaa gaacaaataa gaggcaggtt tagctaaatt aatactcgtt ttgccgtaaa
    11701 aatacgcaag cggtcatttt tttcaaa
//
LOCUS       DANJWC010000072        11659 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.072, whole genome shotgun sequence.
ACCESSION   DANJWC010000072 DANJWC010000000
VERSION     DANJWC010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11659
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.072"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            10..1008
                     /gene="yiaK"
                     /locus_tag="PWX02_001372"
     CDS             10..1008
                     /gene="yiaK"
                     /locus_tag="PWX02_001372"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HDL1729381.1"
                     /translation="MRVSYEELKAQFKRVLLARNVREEIAEECATVFADTTQAGAYSH
                     GVNRFPRFIQQLENGDIVPEAVPTKVLSLGAIEQWDAHQAIGNLTAKKMMDRAMELAS
                     ENGIGVVALRNANHWMRGGSYGWQAAEKGYIGICWTNALAVMPPWGAKECRIGTNPLI
                     IAVPTNPITMVDMSCSMYSYGMLEVHRLAGRQTFVDAGFDDDGNPTRDPATVEKNRRL
                     MPMGFWKGSGLSIVLDMIATLLSNGESTAAVTEDKDDEYCVSQVYIAIEVDRLIDGKT
                     KDEKLNRIMEYVKTAERADPDVAVRLPGHEFTQILADNKANGIPVDDTVWAKLQAL"
     gene            1133..1606
                     /locus_tag="PWX02_001373"
     CDS             1133..1606
                     /locus_tag="PWX02_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDL1729382.1"
                     /translation="MFFGHISQVKANRYPPAIQIALNYLKNTDFDAMEAGVYELKGRQ
                     IYVQVLDLNTKSKHEFQPEVHRNYLDVQYLHRGKEIMAAAVDTGTNPIAMEYNPERDI
                     QYYQSVANENEFRCVEGNFAVFFPEDCHRTAIFDDTETIRKVVVKIAMSEIEEQR"
     gene            1603..2082
                     /locus_tag="PWX02_001374"
     CDS             1603..2082
                     /locus_tag="PWX02_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL1729383.1"
                     /translation="MKALAQLVGKLLEILVVVILSAMSILVFVNVVLRYGFNSSISVT
                     EEVSRYMFVWLAFLGAVLAFSENQHVKVTMLTDKLSPTARNILSVVTDLAMLFCCYLI
                     VDGSWIQFNLNLNNFAPISGLPQGITFLASTVAGVLIGLLIIARIVATVGVIAKGEK"
     gene            2083..3360
                     /locus_tag="PWX02_001375"
     CDS             2083..3360
                     /locus_tag="PWX02_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017806296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HDL1729384.1"
                     /translation="MTVVIFLSVLLGSILLGIPVAFALLVCGVALMLHLDLFDAQILA
                     QQIVSGADSFSLMAIPFFILAGEIMNEGGLSKRIIDLPMKLVGHKRGGLGFVAILAAM
                     IMASLSGSAVADTAAVAAMLLPMMKTTGYPIDRSAGLIGTAGIIAPIIPPSIPFIVFG
                     VASGVSITKLFLAGIFPGIMMGICLAALWWWQAKRLDLMTFSKATKEELCISFKNSIW
                     ALLLPVIIIGGFRSGMFTPTEAGAVATFYALIVSLFVYREMKFKDLYKVVLAAAKTTA
                     VVMFLVAAANVTGWLITVAELPMMLTELLEPLIESPTTLLLVIMLAVFIIGMVMDLTP
                     TVLILTPVLMPLVEEAGIDPVYFGVLFILNTSIGLITPPVGNVLNVITGVSKLPFDQA
                     AKGVMPYLVMMICLLLLFVFFPSLVLVPLSWMQ"
     gene            3463..4449
                     /locus_tag="PWX02_001376"
     CDS             3463..4449
                     /locus_tag="PWX02_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL1729385.1"
                     /translation="MKLFNLKTLVALVAGVAVMSGSAMAETSLRFGYEAPRSDTQHIA
                     AKKFDELLAAKTKGEIKLKLFPDSTLGNAQTMISGVRGGTIDIEMSGSPNFTGLEPKL
                     NVIDIPFIFKDREHVYKVLDGEVGQGLLKDLEAQGLKGLAFWDVGFRAFSNSKHPVTK
                     PEDIKGLKVRTNQNPMYIQAFTLLGGNPVPMPLSELYTALETRAVDAQEHPIGIFWSA
                     KLFEVQKHLSLTNHGYTPLIVVMNKAKFDGLAPELQTALVEAAKEAGQFQRDLNVKNE
                     KEIIEKLRKQGIEVIEQVDNAPFKAAIEAEIRKAFVEKHGDELLKKVDALAQ"
     gene            4523..5506
                     /locus_tag="PWX02_001377"
     CDS             4523..5506
                     /locus_tag="PWX02_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL1729386.1"
                     /translation="MKLFNLKTLAAFVAGFALFSTAQAETSFRFAYEAPRSDTQHIAA
                     KKFNDLLQEKSQKGLKLSLFPDSTLGNAQTAISGVRGGTIDIAMSSSSNFTGLEPKLN
                     VIDIPFIFKDRDHAYKALDGEIGQGLLTALEAHGLKGLAFWEVGFRAFSNSKHPITKP
                     EDIKGLKIRTNQNPMYIQAFSILGANPVPMPLSELYTALETKAVDAQEHPIGIFWSAK
                     LYEVQKYFSFTNHGYTPLIVAINKAKFDALPAEQQTALVEAAKEAGKYQRQLNLENEQ
                     KIIDSLKKAGIEFVDNLDTTPFKDAVSAETRKAFIEKNGDGLVKAIDALAK"
     gene            5637..7121
                     /locus_tag="PWX02_001378"
     CDS             5637..7121
                     /locus_tag="PWX02_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL1729387.1"
                     /translation="MQHIIKRFGSHTAVNDVSLDIHSGEIVALLGENGAGKSTLIKIL
                     AGIYDRDEGDILFHNQKIQSAHAIEKGNKKPIAFIHQDLGLIEWMTIAENMAFVMGFP
                     RKFGLIDWAKVREQSQKALDFVGIKLSADTRVFDLSRTEKALLAIARAIAVNAEILVL
                     DEPTASLPASDVEHLFTVLNRLRSQGVGMIYVTHRLDEVIAISDRILVMRDGFPVAEG
                     QTKLYDVKGLVKAIVGEESRGRQRVDLPTDTPEILSLQNVMVGDTGPVNFRLHKGEMI
                     ALAGLRGAGQEEIGRLLFGERTLDKGEIQLDKRSYSAKDPQDAIAKGVALVAGDRTRE
                     SVFMSMTTSENLFINPVLSGHSPLKRYSRREEWGESWYKFQLFDIRPKNLFIDASALS
                     GGNQQKIVLARWMLLNTPVLILEDPTAGVDVGARAEIYDLLNKALAEGVAIIVISTDF
                     EEIAHLCNRALVFNRGEIAGELLNEEVTFANLLALASDSQSKKQ"
     gene            7316..8362
                     /locus_tag="PWX02_001379"
     CDS             7316..8362
                     /locus_tag="PWX02_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1729388.1"
                     /translation="MAQTNIKSTALEQPVSIARDGFMAWFSQMSTRYGLLWLCVLLVI
                     IFSLTTESFASMLTLNAILESKSKIALLALAATTTMIVGKIDLNVGFGIVLWHILVIT
                     LQVQYGFSWYMAILIVLVIAALYGLLNGILVALADIDSFVATLGSGTVLYAVALWHSG
                     GRQIVGDLPDEFIALNSTEIFGIPISAFYVLAVAIVMWLVTEHTPTGRCMYAVGGNPT
                     AARLNGINIKKYTIVPFIVSSVITAFTGVLISAQQGVGQASVGMDYLLPALVGAFLGS
                     TTIRPGRINVWGTVVGIAILAIGISGIQQFGGAFWVEPLFNGATLLLSITIAGYAQRK
                     RLLNQKAVQKKSTN"
     gene            8375..9484
                     /locus_tag="PWX02_001380"
     CDS             8375..9484
                     /locus_tag="PWX02_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL1729389.1"
                     /translation="MKKAFRTTLTAIATLTAIAFGNNAFANDDPFVQEAKQLVAAATA
                     KQEKWDGPTTGPQLQKEKTVIFIASDMKNGGVLGVIDGMKEAVGVTGWKLDVLDGAGT
                     VNNQLAALNQAIARKPDAIVIGGWNPNVAKIPLQKATKNGIKLAAWHATPEPGAIDKY
                     GIFYNVTSDSDEIARLSAMLAVAESNGKAKAIILTDSLYEIALRKANVMKEVIEKCKE
                     CKVLEFIDTPLADTSSRMPNLTFSLLQKYGDDLEYALAINDLYFDFMAPSLRTAGKDK
                     PFSISAGDGSVTAYQRIRNGDKQFATVPEPLNLHGWQLVDELNRAFANEKPSGYITPA
                     HLVTKENVAFDGGENNLYAPQNGYKGAYKAIWGVK"
     gene            9589..10479
                     /locus_tag="PWX02_001381"
     CDS             9589..10479
                     /locus_tag="PWX02_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HDL1729390.1"
                     /translation="MSNKFIPKISNPRFNELVGNTFELEELFDKAIWAEGPAWSKPQQ
                     ALYFSDVKGCTMYRWTEQDGTQVFRQPSYFSNGNAFDANDNLITCEHGNRGISLTTPS
                     GEYRFLVGQLDGKRLNSPNDVVVKSDGIIWFTDPPYGILSDNEGKQSPSEIIGCYIYC
                     YDPKTDKLNIATFNTMRPNGLAFSSDEKQLFVADMSSVEFDEDGLHHLVVFDVDGNKL
                     KNRRDVAEISPGIPDGFRIKQNGVIFCSCEEGIIALLPDGTELGRFVLGKLTSNCSFG
                     DDEQTLFITCSNSVYRLTIK"
     gene            10551..>11659
                     /locus_tag="PWX02_001382"
     CDS             10551..>11659
                     /locus_tag="PWX02_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDL1729391.1"
                     /translation="MDNKMNYTLGIDCGGTFIKAALFDTQGNIQALHRENVSVISEQA
                     GYAERDMSQLWAICAEVVRSTIEKSGVNPADIKAVGISAQGKGAFLLDKQNRPLGRAI
                     LSSDQRSLEIVKQWQAEGIPQQLYPITRQTLWTGHPVSILKWVQQNEPERYAQIGAVL
                     MSHDYLRFCLTGELHCEETNISESNLYNMEKGEYDQQLADLLGMPDVLEKLPPVIEPN
                     KISGYVTENAAKLTGLAVGTPVVGGLFDVVSTARCANLDDETKLNAVLGTWSVVSGIT
                     DHIDASQSLPFVYGRYAEAGKFIVHEASPTSAGNLEWFVNQWKLDYVKINQAVAQLPP
                     AASSVLFVPFLYGSNAGLGMQAGFYGMQAHHTQAHL"
BASE COUNT         3217 a         2535 c         2820 g         3087 t
ORIGIN      
        1 aagatgacta ttcgagtttc ctatgaagaa ttaaaagctc aatttaaacg tgttctgttg
       61 gctcgcaatg tgcgtgaaga gattgccgaa gagtgtgcca ctgtgtttgc cgacaccact
      121 caagcgggtg cttattcaca cggggtaaac cgtttcccac gctttattca gcagttagag
      181 aacggtgata ttgtgccgga agccgtgccg actaaagtgc tttcactggg ggcgattgag
      241 cagtgggacg ctcaccaagc catcggtaac ttaaccgcaa aaaaaatgat ggacagagca
      301 atggagcttg catctgaaaa cggcatcggc gtagtggcgt tacgtaatgc aaaccactgg
      361 atgcgtggcg gttcttacgg atggcaggcg gcagagaaag gctatatcgg gatctgctgg
      421 acgaacgccc ttgcggtaat gccgccttgg ggggcgaaag agtgccgtat cgggacgaac
      481 ccgttaatca ttgcggtgcc gactaaccca atcacgatgg tggatatgtc ttgctcaatg
      541 tactcatacg gaatgttgga agtgcatcgc cttgctggtc gtcaaacctt tgtcgatgct
      601 ggttttgatg atgacggcaa cccaacccgt gatccggcaa cggtcgagaa aaaccgccgt
      661 ctaatgccga tgggcttctg gaaaggttcg ggcttatcga ttgtgttaga tatgatcgcc
      721 accttgctct ctaacggtga atccactgcg gcagtcaccg aagataaaga cgatgaatat
      781 tgcgtgtcgc aagtttatat cgctattgaa gtggatcgct taattgacgg caaaaccaaa
      841 gacgaaaaac tcaaccgtat tatggaatat gtgaaaaccg cagaacgtgc cgaccctgat
      901 gtggcagtac gtttaccggg ccacgagttc acccaaattc tagccgataa caaagccaac
      961 ggcattccgg tggatgacac tgtttgggcg aaattacagg cgttgtaatt gcaaattgac
     1021 cggtagggtg ggcgtaagcc cacgcattag acacaatcat aagatgacgt gggcttacgc
     1081 ccaccctaca agcggtcatt tttccgcaga attttacaaa acggaggtat gaatgttttt
     1141 tggtcatatt tcacaagtta aagcgaatcg atacccgccg gcgatccaaa ttgcgttgaa
     1201 ctatctgaaa aataccgatt ttgatgcgat ggaagcaggg gtttacgaac tcaaaggcag
     1261 acaaatttat gtgcaggtgt tggatttgaa caccaaatcg aaacacgaat ttcagccgga
     1321 agtgcatcgc aattacctcg atgtgcagta tctacaccga ggcaaagaga taatggctgc
     1381 ggcggtggat accggcacca atccgattgc gatggaatac aaccctgagc gggatattca
     1441 atattatcaa tccgttgcca acgaaaatga atttcgctgc gttgaaggca attttgcggt
     1501 gtttttcccc gaagattgcc acagaacggc aatttttgac gacacggaaa ccattcgcaa
     1561 agtggtggta aaaattgcga tgagcgaaat tgaggagcaa cgatgaaagc cttagctcaa
     1621 ttagtcggaa aactgttaga aatcttggtg gtggtgattt tatccgcaat gtcgattttg
     1681 gttttcgtca atgtggtact acgttacggt tttaacagca gcattagcgt gaccgaagag
     1741 gtgtctcgct atatgtttgt ttggttggcg tttttagggg cggtgttggc atttagcgaa
     1801 aatcaacacg ttaaagtcac aatgctgacc gataaattat cgccaacggc tcgtaatatc
     1861 ttaagcgtag tcacagattt agcgatgttg ttctgttgct acttaattgt ggacggtagc
     1921 tggattcaat ttaacctcaa cctgaataac tttgccccga tttcaggctt accgcaaggc
     1981 atcactttcc ttgccagcac agtggcaggg gtattaatcg gtttattaat tattgcccgc
     2041 attgtggcaa cggttggcgt gattgcaaaa ggagaaaaat agatgactgt ggtaattttt
     2101 ctttccgtct tgcttggctc gattttattg ggcattccag tagctttcgc cctgttggtc
     2161 tgcggtgtgg cgttaatgtt gcatttagat ctgttcgatg cccaaatttt ggctcaacaa
     2221 attgtgagcg gtgcagacag tttctcgcta atggcaattc cgttcttcat tcttgccggt
     2281 gaaattatga acgagggcgg tctctccaaa cggattatcg acctgccgat gaaattagtc
     2341 ggtcataaac ggggcggttt aggttttgtg gcaattttag cggcgatgat tatggcaagc
     2401 ctttccggtt cggcggtggc ggataccgca gcggtagccg caatgctact gccgatgatg
     2461 aaaaccaccg gttatccgat tgaccgctct gccggtttaa tcggtacggc agggattatc
     2521 gccccaatca ttccgccaag cattccgttt atcgtatttg gtgtggctag tggcgtgtcc
     2581 attaccaaac tgttcttagc cggtattttc ccgggcatta tgatggggat ctgcttggct
     2641 gccctttggt ggtggcaggc aaaacgtttg gatttaatga ccttctccaa agcgaccaaa
     2701 gaagaacttt gcatttcatt taaaaacagt atttgggcgt tattgttgcc tgtcattatt
     2761 atcggtggtt tccgctcggg aatgttcacc ccaaccgaag cgggtgcggt cgcaactttc
     2821 tatgccttga ttgtttcact gtttgtttac cgtgaaatga agttcaaaga tttatacaag
     2881 gtagtgttgg cggcagcgaa aaccacggca gtagtgatgt tcttagtggc agcggcaaat
     2941 gtgaccggct ggctgattac cgtagcggaa ttaccgatga tgctcaccga gctgttagag
     3001 ccgttaattg aaagcccgac cacactctta ttggtgatta tgttagcggt gtttattatc
     3061 ggaatggtga tggacttaac cccaaccgtg cttattttaa cgccggtatt aatgccgttg
     3121 gtggaagagg caggcattga cccggtttac ttcggtgtgc tgtttattct taacacctcc
     3181 atcggcttga ttacaccacc ggtcggcaac gtgctaaacg tgattaccgg cgtgtcgaaa
     3241 ctgccgttcg accaagcggc taagggcgta atgccttacc tcgtaatgat gatttgttta
     3301 ctactgttgt tcgtgttctt cccgtcactg gtgttagtgc cgttaagctg gatgcagtaa
     3361 tggtttgaac cgaaacaagc ggtcgaattg acgcaaaaat ttacaaattt ttttggcaaa
     3421 acagaccgct tgcaactcaa tttttaatca caggagaacc ctatgaaatt atttaattta
     3481 aaaaccttag ttgccttagt ggcaggcgtt gcggtaatga gcggctctgc aatggcagaa
     3541 acctcactgc gttttggtta tgaagcccca cgttccgata cccaacacat cgccgccaaa
     3601 aaattcgatg aattattggc agcaaaaacc aaaggtgaaa ttaaattaaa actgtttccg
     3661 gacagcacct taggtaatgc tcaaacgatg atcagcggcg tgcgtggcgg tactatcgac
     3721 attgaaatgt ccggctcgcc aaactttacc ggcttagagc caaaactaaa tgtgattgat
     3781 attccgttta ttttcaaaga tcgtgaacac gtttacaaag tattagacgg cgaagttggt
     3841 caaggcttat taaaagattt agaagcccaa ggcttaaaag gcttagcctt ctgggacgtt
     3901 ggcttccgtg ctttctccaa ctcaaaacac cctgtgacca agccggaaga catcaaaggc
     3961 ttaaaagtcc gcaccaacca aaacccgatg tacattcaag ccttcacctt actaggcggc
     4021 aacccggtgc caatgccact ttctgagctt tacaccgcac tcgaaacccg tgccgttgat
     4081 gcccaagaac acccaatcgg cattttctgg tcggcaaaac tgtttgaagt acaaaaacat
     4141 ttaagtttaa ccaaccacgg ctacaccccg ttaatcgtgg tgatgaacaa agcgaagttt
     4201 gacggcttag ctcctgaatt acaaaccgca ttggttgaag ccgcaaaaga agccggtcaa
     4261 ttccaacgtg atctcaacgt gaaaaacgaa aaagagatca tcgaaaaatt acgcaaacaa
     4321 ggcatcgaag tgatcgaaca agtggataac gccccgttca aagcagcgat tgaagcggaa
     4381 atccgcaaag ctttcgtgga aaaacacggc gatgaattat tgaaaaaagt ggatgcgtta
     4441 gcacagtaat tgactttatt ttagtctaaa gctccctccg tttgggggga gttgttgtat
     4501 agactctact ataggagaga ctatgaaact gtttaattta aaaactttag ccgctttcgt
     4561 tgccggtttt gcactgtttt caaccgcaca agcagaaacc tcattccgct ttgcgtatga
     4621 agctcctcgt tctgatactc aacatattgc agcgaaaaaa tttaatgatt tgctgcaaga
     4681 aaaatcccaa aaagggctga aattaagcct cttccctgat agcacgctcg gcaacgcaca
     4741 aacggcgatt agcggtgtgc gtggcggtac tattgatatt gcaatgtcca gctcctctaa
     4801 ctttaccggg ttagaaccaa aactgaatgt gattgatatt ccgtttattt tcaaagacag
     4861 agaccacgcc tataaagcct tagatggaga gattggtcaa ggtttattaa ccgcattaga
     4921 ggctcacggt ttgaaagggt tggcgttttg ggaagttggt ttccgagcgt tttccaactc
     4981 caagcacccg atcaccaaac cggaggatat taaaggctta aaaatccgta ctaaccaaaa
     5041 cccgatgtat attcaagcct tctctatttt aggtgcaaat ccggtgccaa tgccgctttc
     5101 cgagctttac actgcactgg aaaccaaagc ggttgatgcc caagaacacc caatcggcat
     5161 tttctggtcg gcaaaactct atgaagtaca aaaatatttc agctttacta accacggtta
     5221 caccccgtta attgtggcga ttaacaaagc gaaatttgat gcactgccag ccgagcaaca
     5281 aaccgcatta gttgaagcgg caaaagaagc cggaaaatac caacgccaac ttaacctaga
     5341 aaatgagcag aaaattatcg actcgctcaa aaaagcaggt attgaatttg tggataactt
     5401 agataccaca ccgttcaaag atgccgtatc ggctgaaacc cgcaaggcat ttatcgagaa
     5461 aaacggtgat ggcttagtta aagccattga tgcattggcg aagtaaaaat agagctacaa
     5521 agtgctcggt taaactaacc tcacacgaat cgtgtgaggt acaagcggtc aaaatccgca
     5581 aaatctttac acatctttcc tcaaaggagg aacaaatggc acagcccatt ttaagtatgc
     5641 agcacatcat caaacgcttt ggtagccata cggcggtgaa tgatgtcagc cttgatatcc
     5701 actcaggcga gatcgttgcc ttactcggtg aaaacggtgc aggtaaatcg accctaatca
     5761 aaatccttgc cggtatttat gaccgtgacg agggtgatat tcttttccat aaccaaaaaa
     5821 tccaatctgc ccacgctatt gagaaaggca acaaaaagcc catcgctttt atccaccaag
     5881 atttaggcct aatagaatgg atgacgattg ccgaaaatat ggcatttgta atggggttcc
     5941 ctcgcaaatt tggcttgatt gactgggcga aagtgcgaga gcagtcgcaa aaagcccttg
     6001 attttgtagg cattaaactc tctgccgaca ctcgagtgtt tgatttaagc cgtaccgaaa
     6061 aagccttact cgccattgcc cgtgcgattg ccgtcaatgc agaaatcttg gttttagacg
     6121 aacctactgc ttccctacct gcctctgatg tagagcatct tttcactgtg ttaaaccgct
     6181 tacgttccca aggtgtggga atgatttatg tgacccaccg tttagatgaa gtgatcgcca
     6241 tttccgaccg cattttggtg atgcgggacg gtttcccggt tgcagaaggg caaaccaagc
     6301 tctatgacgt caaaggcttg gtcaaagcga ttgtaggcga agagagccgt ggcagacagc
     6361 gggtggattt accaaccgat actcctgaaa ttttaagcct gcaaaatgtc atggttggcg
     6421 acaccggccc ggtgaatttc cgtctgcaca aaggtgagat gattgccctt gccggcttgc
     6481 gtggagcagg gcaggaagaa atcggccgct tattatttgg ggagcgaacc ttagacaaag
     6541 gcgaaattca gttagacaag cggtcatatt ctgctaaaga tccgcaagat gcgatagcca
     6601 aaggtgtggc attggttgcg ggagaccgca cccgtgaaag cgtgtttatg tcaatgacta
     6661 ccagtgagaa cctgtttatc aacccggtac taagcggtca ttcaccatta aaacgctact
     6721 cccgccgtga agagtggggt gagagctggt acaaattcca actattcgat attcgtccga
     6781 aaaatttgtt tattgatgcc agtgcgttat ccggtggtaa tcagcagaaa atcgtgttgg
     6841 cacgttggat gctcctcaat acgcctgttc tgattttaga agacccgact gccggcgtgg
     6901 atgtaggagc aagggcagag atttacgact tgctcaacaa agcccttgca gaaggggtcg
     6961 caattattgt gatttccacc gactttgaag agatcgctca tttgtgtaac cgagcattag
     7021 tcttcaaccg aggggaaatt gccggtgaac tactgaacga agaggtgact ttcgccaatt
     7081 tgctggcgtt ggcatcggat agtcagtcga aaaaacaata acttactctc gcccgcttgc
     7141 gggagagaga cagctaaaaa tagcgtcagg ctatttttag cagagagagg gtaaacaagc
     7201 ggtcattttt cttaaaaaat attgcgaacc ctctccctcc gaaagcctaa cggctttctc
     7261 ctccctctcc cgcaagcggg cgaggggaaa atgcagaatt ttaacggagt aatttatggc
     7321 tcaaacaaac atcaaatcaa ccgcactgga acaaccggta tctatcgccc gtgacggttt
     7381 tatggcatgg ttttcacaaa tgtccactcg ttatggctta ctgtggttat gtgtgttatt
     7441 ggtgattatt ttctcactta ccaccgaatc tttcgcctca atgctgacat taaatgcgat
     7501 tttagaaagt aaatcgaaaa ttgccctact tgctttggca gccacaacta caatgattgt
     7561 cggcaaaatc gacctgaacg tgggtttcgg tattgtgttg tggcacattc ttgtgattac
     7621 tctgcaagtc caatacggct tctcgtggta tatggcgatc ttaatcgtgt tagtgattgc
     7681 cgctctttac ggcttgctga atgggatttt agttgctctt gctgatatcg atagctttgt
     7741 ggcaacactt ggctcaggca cagtgcttta tgccgtagca ctctggcact cgggcggtcg
     7801 ccaaattgtc ggggatttac ccgatgaatt tatcgcactc aacagcacag aaattttcgg
     7861 tatcccgatt tctgctttct atgtgctggc agtggctatc gtaatgtggt tggtgacaga
     7921 acataccccg accggtcgtt gtatgtatgc ggttggtggc aacccaacgg cagctcgcct
     7981 aaatggtatc aatattaaaa aatacaccat tgtgccgttt attgtctcta gtgtaatcac
     8041 tgctttcaca ggcgtattga tctctgctca acaaggcgtt ggtcaggcaa gtgtcggtat
     8101 ggactactta ctgcccgcat tggttggggc attcttaggg agtaccacta ttcgcccagg
     8161 gcgtattaac gtatggggaa cagtggtggg gattgccatt cttgccatcg gtatttcagg
     8221 tattcaacaa ttcggtggag cgttctgggt tgagccactc tttaacggtg caacactgtt
     8281 actttccatc actattgcag gctatgccca acgcaaacgc ttgttaaatc aaaaagctgt
     8341 acagaaaaaa tcgaccaact aagaggattt gattatgaaa aaagcatttc gtacaacctt
     8401 aactgcaatc gcaaccttaa ctgctattgc tttcggaaat aatgcgtttg ccaatgacga
     8461 cccgttcgta caagaagcaa aacagcttgt cgcagcagct acggctaagc aagaaaaatg
     8521 ggacggaccg acaaccggcc ctcaattaca aaaagagaaa actgtgattt ttattgcctc
     8581 tgatatgaaa aacggtggtg tacttggtgt gattgacgga atgaaagaag ctgttggcgt
     8641 aacgggctgg aagttagatg tattagacgg tgcaggtacg gtaaacaacc aactcgccgc
     8701 acttaaccaa gcgattgccc gcaaaccgga tgcgattgtg attggtggct ggaacccgaa
     8761 tgtggcgaaa atcccactac aaaaagcgac taaaaacggc attaaacttg cagcttggca
     8821 cgcaacaccg gagccgggtg cgattgataa atacggcatt ttctacaacg tgacctctga
     8881 ctcagacgaa attgcacgct tatcggcaat gcttgcggtg gcagaatcta acggtaaggc
     8941 aaaagcgatt attttaaccg actcgctata tgaaattgcg ttacgcaaag cgaacgtcat
     9001 gaaagaagtt atcgaaaaat gtaaagagtg taaggtgtta gagtttatcg acacgccatt
     9061 ggcggatacc tcaagccgta tgcctaacct cactttcagc ttactgcaaa aatatggcga
     9121 cgatttagag tatgcgttgg caattaacga tctctacttt gactttatgg ctccgtcttt
     9181 acgcaccgct ggtaaagata aaccgtttag catttccgca ggtgatggct cagtaacggc
     9241 ttatcaacgt attcgcaatg gtgataaaca gtttgcaacc gtccctgagc cacttaactt
     9301 acacggctgg cagttagtcg atgagctaaa ccgtgctttc gctaacgaaa aaccgtctgg
     9361 ttatatcaca ccggctcacc tagtgactaa agaaaatgtg gcgtttgacg gtggtgaaaa
     9421 caacctgtat gccccacaaa acggttacaa aggcgcttac aaagcgattt ggggcgtgaa
     9481 gtaatgacgt aggggcaaat aaatatttgc ctcgaacaag cggttgtttt ttgcaaagat
     9541 tttgcgaaat ttaaccgctt gtcataaaaa atagagaggg taatacaaat gagcaataaa
     9601 tttatcccca aaatctccaa ccctcgtttt aatgagctgg ttgggaatac gtttgaactt
     9661 gaagagcttt ttgataaggc aatctgggca gaagggccgg cgtggagcaa gccacaacaa
     9721 gccctctatt ttagtgatgt aaaaggttgc acaatgtacc gctggacaga gcaagatggt
     9781 acacaggttt tccgccagcc gtcttatttt tctaacggca atgcgtttga tgcaaacgac
     9841 aacttgatta cctgtgaaca cggcaatcga ggtatcagtt taaccacgcc aagcggtgaa
     9901 tatcgttttt tggtcggtca attagacgga aaacgactga attcacccaa tgatgtggtg
     9961 gtaaaatcag acggcatcat ttggttcact gatccacctt acggtatttt gtcggataac
    10021 gaaggcaaac agtcgccaag cgaaattatc ggttgttata tttactgcta tgatccgaaa
    10081 accgacaagc tgaatattgc aactttcaac acgatgcgac caaacggctt ggccttctcg
    10141 tcagacgaaa aacaactctt tgtggcagat atgtcgagcg tggaatttga cgaagatggc
    10201 ttacaccact tagtcgtatt tgatgtggac ggtaataaac taaaaaatcg ccgagatgtg
    10261 gcggaaatta gtcccggcat tccggacggc ttccgaatta aacaaaacgg cgtaattttc
    10321 tgctcttgtg aagagggaat tattgctctt ttacctgacg gcacggagct gggacgattt
    10381 gtgctgggta agctaacctc aaattgtagc tttggcgatg atgaacaaac actgtttatt
    10441 acttgttcga atagcgttta tcgtttaaca attaaatagc agaatgcatt aagtgcgtct
    10501 gttgtagcat tacaagcggt ttctttttca aaatttttta caaacaaata atggataaca
    10561 aaatgaacta cacactcggc attgattgcg gtgggacttt tatcaaagcc gctctttttg
    10621 atacacaagg taatattcaa gcccttcatc gtgaaaacgt ttcggtaatc agtgaacagg
    10681 caggttatgc agagcgtgat atgtcacaac tctgggctat ttgtgcggaa gtagtgcgtt
    10741 caaccattga aaaaagtggg gtaaacccag cggatattaa agcagtaggg atttccgctc
    10801 aaggcaaagg ggcgttttta ctggataagc agaatcgacc gttggggcgt gcgattttat
    10861 cttccgacca acgttccctt gagattgtta aacaatggca ggctgaaggc attccgcaac
    10921 aactttaccc gattacccgc caaactcttt ggacaggaca ccctgtgtcg attttgaagt
    10981 gggtgcagca aaatgaaccg gagcgttatg cacaaatcgg ggcggtgttg atgtcgcacg
    11041 actatttgcg tttttgctta accggtgagt tgcattgcga agagaccaac atttccgaaa
    11101 gcaatcttta caatatggaa aaaggcgaat acgaccaaca gttagccgat ttgttgggaa
    11161 tgccggatgt gttggagaaa ttaccaccag tgattgaacc gaataaaatt tcgggatatg
    11221 tgactgaaaa tgccgccaaa ttaaccggtt tagcggtggg tacgccggta gtcggtggct
    11281 tgtttgatgt ggtttctacc gcacgttgtg ccaatttaga tgacgaaacc aaactgaatg
    11341 cggtgctggg aacgtggtcg gtggtgagcg gcattaccga tcatattgat gcaagccaat
    11401 cattaccttt tgtttatggg cgttatgccg aggcagggaa atttatcgtc cacgaagcaa
    11461 gcccgacttc tgccggtaat ttagagtggt ttgtgaatca gtggaaactc gattatgtga
    11521 aaatcaacca agctgttgcc caattaccgc cggcggcaag ctcggtgctg tttgtgccgt
    11581 ttctgtatgg ctcaaatgca ggcttaggaa tgcaagccgg tttctatggt atgcaagccc
    11641 accataccca agcccatct
//
LOCUS       DANJWC010000073        11409 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.073, whole genome shotgun sequence.
ACCESSION   DANJWC010000073 DANJWC010000000
VERSION     DANJWC010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11409
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.073"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(175..480)
                     /locus_tag="PWX02_001383"
     CDS             complement(175..480)
                     /locus_tag="PWX02_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729392.1"
                     /translation="MSKLNKLLSRKTSRKIIPEQVNFDINLPIYIPLFLSDKTAKIQA
                     VWELEQENGERDAGAVKRNRIQFRYLPEGVHKLTLIVGTPLLSKGSKVYQCLIKVNK"
     gene            855..1970
                     /gene="asd"
                     /locus_tag="PWX02_001384"
     CDS             855..1970
                     /gene="asd"
                     /locus_tag="PWX02_001384"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDL1729393.1"
                     /translation="MQNVGFIGWRGMVGSVLMDRMVEENNFANINPVFFTTSQAGQKA
                     PVFGGKDAGELKNAFDIEELKKLDIIVTCQGGDYTNEVYPKLKETGWNGYWIDAASAL
                     RMKDDAIIVLDPVNQHVISEGLKNGIKTFVGGNCTVSLMLMAIGGLFEKDLVEWVSVA
                     TYQAASGAGAKNMRELLSQMGELEDSVKAELANPASSILEIERKVTAKMRAEDFPTEN
                     FGAALGGSLIPWIDKLLPETGQTKEEWKGYAETNKILGLSDNPIPVDGLCVRIGALRC
                     HSQAFTIKLKKDIPLAEIEQIIAAHNEWVKVIPNDKETTLRELTPAKVTGTLSVPVGR
                     LRKLAMGGEYLAAFTVGDQLLWGAAEPVRRILVQLTQ"
     gene            2080..3006
                     /locus_tag="PWX02_001385"
     CDS             2080..3006
                     /locus_tag="PWX02_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL1729394.1"
                     /translation="MKPIFLEIRHLRTLIALKESGSVSLAAKRVHLTQSALSHQIKLL
                     EEQYDLTLFERKSSPIRFTPAGERLIKLAYDILPKVIEAELDLARVKQGEVGEVRVAV
                     ECHTCFDWLMPAMDTFRQHWPLVELDIVSGFHTDTVGLLLSHRADLAIVAEEEDSAGI
                     IYKPLFSYEMVAICSKDHPLATKDVWEAEDFKDETLITYPVPDDMLDLLRKILGPAGI
                     KVTRRNSELTIAIIQLVASRRGIAALPYWAVKPYLDRGYVVARKITQQGLYSTLYGAI
                     READATLAYLDDFHDTVKSQSFATLPGLMVLE"
     gene            complement(3073..4518)
                     /gene="pntB"
                     /locus_tag="PWX02_001386"
     CDS             complement(3073..4518)
                     /gene="pntB"
                     /locus_tag="PWX02_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HDL1729395.1"
                     /translation="MSFGFVTAAYIIAAILFIMSLAGLSKHETAKAGCWYGIVGMAIA
                     LVATIFGPQSHGTIWILIAMAIGGFIGVKKALKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYGLHTNALMPANLDETAQAAFLAEQATLANIHNVEVFLGIFIGAVTFSGSLVAFG
                     KLSGKLFGRKVSSAALNIPHKHKWNLAAIIVSVFLMIVFLNNPHNIFPVLIMTIIALV
                     FGWHLVSSIGGADMPVVVSMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYI
                     MCKAMNRSFISVIAGGFGNEVQSGKGDEEYGEYRETTAEEVAEMLKNASSVIITPGYG
                     MAVAQAQYPVAEITAKLREKGVNVRFGIHPVAGRLPGHMNVLLAEAKVPYDVVLEMDE
                     INDDFEETDVVLVIGANDTVNPAALDDPSSPIAGMPVLEVWKAQNVIVFKRSMAVGYA
                     GVQNPLFFKENTQMLFGDAKERVDDILRALN"
     gene            complement(4533..6071)
                     /gene="pntA"
                     /locus_tag="PWX02_001387"
     CDS             complement(4533..6071)
                     /gene="pntA"
                     /locus_tag="PWX02_001387"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HDL1729396.1"
                     /translation="MLIGVPRELLDGETRVAATPKTVEQIKKLGFDVLVEQDAGFKAS
                     FEDNAFLNAGANIGTQQEVWNADIIFKVNAPTEAEIELIKEGVTLVSFIWPAQNPQLM
                     EKLQSKKINVLAMDAVPRISRAQALDALSSMANISGYRAVIEAANAFGSFFTGQITAA
                     GKVPPAKVLVIGAGVAGLAAIGAANSLGAIVRAFDSRPEVKEQVKSMGADFLEIDFEE
                     EGGSGDGYAKVMSEEFNRRAMALYAEQAKEVDIIITTAAIPGKPAPRLITKEMVDSMK
                     PGSVIVDLAAATGGNCEYTKAGEIVVTDNQVKVIGYTDFPARLPTQSSQLYGTNLVNL
                     LKLLAPNKDGQIEINFDDVVLRGVTVVRDGELTWPAPPIQVSAQPQKQAAAPQAVKKE
                     EKPADPRVKYGVMAAAGVLFLWLASVAPAAFLSHFTVFVLACVVGYYVVWNVSHALHT
                     PLMAVTNAISGIIIVGAVLQIAQPTGNFFIDILAFIAILVASINIFGGFKVTQRMLAM
                     FRKG"
     gene            6251..6967
                     /gene="trmJ"
                     /locus_tag="PWX02_001388"
     CDS             6251..6967
                     /gene="trmJ"
                     /locus_tag="PWX02_001388"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HDL1729397.1"
                     /translation="MQILNQIQIILVETSLPANIGSAARAMKTMGLTHLRLVAPKQPL
                     DEQAEALAAGAKDVLDNAQCYATFDEAIADCQLVIGTSARLRHLQNTLIEPRDCGELV
                     VNRANLGKVAIVFGRERVGLTNEELLKCQYHLNFPTNPDYGSLNLAMAVQLASYEIRM
                     AYLQNSGEKQPLAADKDYPTAQALEHFFAHTERLYTELGFIRNDSVMLKLRRLYQRAE
                     LETNELNLLRGMLTAVEKAN"
     gene            complement(7054..9555)
                     /locus_tag="PWX02_001389"
     CDS             complement(7054..9555)
                     /locus_tag="PWX02_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HDL1729398.1"
                     /translation="MSRQSLSPEMIKNVKNAILHKMVFALGVEPRDASKRNWLNATLR
                     VVRDLSTEDWLQTRRSQVANGSRRVYYLSMEFLMGRTFSNAMISEGVYELIGAALKEL
                     GQDLEEIINEEGDPGLGNGGLGRLAACYMDSLATMKIPAIGYGIQYEYGMFRQEIRNG
                     EQIEQPDEWLENEFVWPYLRSSKRFPVRFGGKTWREGKKVVWQPEEEITAQAHDQLIP
                     GFETTSTNSLCLWSAHASGRGFGLSDFNRGDYFAAMVKQNSSENVSRVLYPDDSTYNG
                     RELRLRQEYFLCSASVQDIIRRHEAEFGSCVNLADKVAIHLNDTHPTLAVPELMRILI
                     DEKGYGWEQAWAMTRKIFFYTNHTLMSEALETWPVEMLARVLPRHLEIIFDINEYFLE
                     EVRSQFPEDHDLISRVSLIDEQGDRRVRMAWLAVVASAKVNGVAKIHSDLMVESIFAD
                     FARIYPNRFTNVTNGVTPRRWIKIANPGLANILDKCIGNKWLTDLSELEKFNVFVDDA
                     DVQAQVAAVKVENKRRLAKYVEETQGIKLNPEAIFDVQVKRIHKYKRQQMNVLHIITL
                     YNRILKNPTADWTPRVFIFAGKAASAYYAAKKVIRLINDVANVVNNDPRINDLIKVVF
                     IPNYGVSLAQMIIPAADVSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEILDH
                     VGKENIFIFGNEVEQVEELRRNGYSPYHYYEKDSELNEAISQILNGKFSPEDPYRYQD
                     LILNSGDYYQACADYRSYVEAQEKVAQAYRNKKAWTRSAIINIANMGYFSSDRSVMDY
                     AKNIWHIEPMSEEQLKGDSTMGSIIESSNKLLNLK"
     gene            complement(9650..11086)
                     /gene="glgA"
                     /locus_tag="PWX02_001390"
     CDS             complement(9650..11086)
                     /gene="glgA"
                     /locus_tag="PWX02_001390"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDL1729399.1"
                     /translation="MKVLHICSEMYPLIKTGGLADVMGALPFAQQQAGNDVRVLIPYY
                     PKVAQTLGETVEVATIGTFAGTVSVRFAYFNGLGVYVIDAPHLFMRDGNPYHDSNYQD
                     YPDNYKRFALLGYIGAQLAQGLDHWWGTAQVLHAHDWQAGLACAYLKSWGSNVKTLFT
                     IHNIAYIGRFNAFHLAELGLPWEFFHAEGLEFYGEISYLKAGLYFADRITTVSPTYAR
                     EITEEIAGAGLHGLLATRQYQGRLRGILNGVDAEVWNPETDTHIVVNYRANYMHGKAK
                     NKAELQRIFGLPEDKEALLMVMVTRLTEQKGADFLLTNIDTLMQHKVQIIVLGSGSPD
                     LENALRYFQHKYPNQIGVKIGYDEALSHQIIAGGDLILVPSRFEPCGLTQLYGLKYGT
                     LPLVRRTGGLADTVVDSHKESIENRSATGFVFDYPDAPAFLEAAMRAISLWQKQKLWS
                     SVRQNALAQDFGWAKVAEQYQNLYHEII"
     gene            complement(11209..>11409)
                     /gene="glgC"
                     /locus_tag="PWX02_001391"
     CDS             complement(11209..>11409)
                     /gene="glgC"
                     /locus_tag="PWX02_001391"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596318.1"
                     /note="catalyzes the formation of ADP-glucose and
                     diphosphate from ATP and alpha-D-glucose 1-phosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDL1729400.1"
                     /translation="IGKNCVIKRAVIDRHCVIPDGLEIGVNAEEDAKRFRVSKNGIVL
                     VTQSMLDKLAGKEANSDIKYTI"
BASE COUNT         3380 a         2540 c         2181 g         3308 t
ORIGIN      
        1 caaaatttta aaaatattgt ataaataata tattatactt ctttctaaat tactatgttg
       61 acgtaattta tctttgattt cttttaaact accctcacca gggtgtttgc tatttttaaa
      121 atggtttctg ttttacaata tacacataaa tacgatcaca acataaagtt aatattattt
      181 attaacttta attaggcatt gataaacctt acttcctttt gataataaag gcgtaccaac
      241 aattaatgtt aatttatgta ccccttctgg caagtatcta aactgaatac gatttcgctt
      301 tacagcgcca gcatcacgct caccattttc ttgttctaac tcccatactg cttgaatttt
      361 cgctgtctta tctgataaaa acagcgggat atagataggc aagttaatgt caaaattaac
      421 ttgctcagga ataattttac gcgatgtttt tctcgataat aatttgttta acttagacat
      481 agaatgttcc tttgtaaaat gtaaaataaa ctcattcatt actgtaaagc ttaaaataaa
      541 agtattagaa ataatagtta aaaacctccc atttaactaa cctagatcaa actttaggta
      601 attacatcta cttcctatcg atatatcggc aaaatttatt gattagacca caattggaat
      661 gcataaattg tgagcatttc cctatttata agattctatt tatttccaaa aatacaccat
      721 aatcgatgtt ttattttaac aatagcggtt aaattttgtc aaaactttgc aaatcaataa
      781 aaaacattga tttttgcact aaaatcctgt atttattcac aatctttata acatcatttt
      841 taggagcaaa cacaatgcaa aatgtaggtt ttatcggttg gcgtggtatg gtcggttcgg
      901 tcttaatgga tcgtatggtt gaagaaaata acttcgccaa tatcaaccct gtttttttca
      961 ctacttcaca agcgggtcaa aaagcccctg tttttggcgg aaaagatgcc ggcgaattaa
     1021 aaaacgcctt tgacattgaa gaattaaaaa aattagacat tatcgtaact tgccaaggtg
     1081 gtgattatac aaatgaagtc tatccaaaat taaaagaaac cggttggaac ggctattgga
     1141 ttgatgcggc ttctgcatta cgtatgaaag acgatgctat tatcgtatta gacccggtga
     1201 accaacatgt gatttcagaa ggcttaaaaa acggcattaa aacatttgta ggcggaaact
     1261 gtaccgtaag cctaatgcta atggctatcg gtggtttatt tgagaaagat ttggtcgagt
     1321 gggtttctgt agcaacttac caagccgctt ccggtgccgg tgcgaaaaat atgcgtgaat
     1381 tgctttctca aatgggtgaa ttagaagata gcgtaaaagc tgaattagca aatcctgcct
     1441 cttctatttt agagattgaa cgtaaagtta cggctaaaat gcgtgcggaa gacttcccaa
     1501 ccgagaactt cggtgcggca ttgggcggta gcttaattcc ttggattgat aaattattac
     1561 cggaaaccgg gcaaactaaa gaagagtgga aaggttatgc agaaactaat aaaattttag
     1621 gtttaagcga taacccaatt ccagttgatg gtttatgcgt gcgtattgga gcattacgct
     1681 gccacagcca agcattcaca attaagctga aaaaagatat tccgttagcg gaaatcgagc
     1741 aaattattgc tgcccacaat gaatgggtga aagtaattcc aaacgacaaa gaaaccacat
     1801 tgcgtgaatt aaccccggcg aaagtgaccg gtacattgag cgtgccggtt ggtcgcttgc
     1861 gtaaattagc gatgggtgga gaatacttag ctgcatttac cgtaggtgac caattattat
     1921 ggggtgcagc agagcctgtt cgccgcattt tagttcaatt aacccaataa ttcgccactg
     1981 ctctatacaa gcggttggat tttgcaaatt ttttgcagaa tttgaccgct tgtttgttat
     2041 aattattttc atcttttttc acttttagat gaataaatta tgaaaccgat ttttcttgaa
     2101 atccgccatt tgcggacatt aatcgcatta aaagagagcg gtagcgtatc tcttgcagca
     2161 aagcgtgtcc atttaacaca atccgcactt tcacaccaaa taaaattact ggaagaacag
     2221 tatgatctta cgctatttga gcgaaaaagc tcccctattc gctttacacc agctggcgaa
     2281 cgtttaatta agttggccta cgatattttg cccaaagtca ttgaggctga attagattta
     2341 gcccgagtga aacaaggaga agtcggagag gtacgtgttg cagtagaatg ccatacctgt
     2401 tttgattggc taatgccggc aatggatact ttccgtcaac attggcctct agttgaatta
     2461 gatatcgtat cgggtttcca tactgatacg gttggtttgc tattaagcca tcgtgcagat
     2521 ctggcaattg tcgctgaaga ggaagatagt gctggtatta tatacaaacc tctcttttca
     2581 tatgaaatgg tagctatttg ctctaaagat caccctcttg ccacaaaaga tgtatgggaa
     2641 gctgaagatt ttaaagatga gacattaatt acctatccag tacctgatga tatgctagat
     2701 ttactgcgaa aaatattagg ccctgctggc atcaaagtta ctcggcgtaa tagtgagtta
     2761 actattgcta ttattcagct agtagcaagt cggcgaggca ttgctgcact accttattgg
     2821 gcggttaaac catatttaga tagggggtat gtggtggcaa gaaaaataac tcagcaagga
     2881 ctatatagca ccctttatgg cgctattcga gaagccgatg ctaccctagc ctacttagat
     2941 gatttccatg ataccgtaaa atcgcagagt tttgcgactc ttcccggatt gatggtatta
     3001 gagtaaaaaa ttaacaattt atgttagcct aagcacaaaa aagcgaactt atgttcgctt
     3061 ttttatttaa atttaattta acgcccgtaa aatatcatca acacgttctt ttgcatcacc
     3121 aaatagcatt tgggtatttt ctttaaagaa cagtgggttt tgcacaccgg cgtagcctac
     3181 tgccattgaa cgtttgaaca cgataacatt ttgtgctttc catacttcaa gcaccggcat
     3241 tccagcaata gggcttgatg ggtcgtctaa tgcagcaggg tttacggtat cgtttgcacc
     3301 gattaccaat accacatcgg tttcttcaaa atcgtcattg atttcgtcca tctccagcac
     3361 tacatcataa ggtactttcg cttccgccaa taatacgttc atatgccccg gtaaacgccc
     3421 tgccacaggg tgaataccaa aacgcacatt cacgcctttt tcacgcaatt ttgcggtgat
     3481 ttccgctacc ggatattgtg cttgagccac tgccatacca taacccggcg tgatgatcac
     3541 agagcttgca tttttcagca tttctgccac ttcttcggct gtcgtttcac ggtattcacc
     3601 gtattcttca tcacctttgc cagactgaac ttcattgcca aaaccaccgg caatcacgct
     3661 aatgaatgag cggttcattg ctttacacat aatataagaa aggatagcac ctgatgatcc
     3721 cactaatgca ccggttacga ttaataaatc gttgcttagc ataaagcctg ccgctgcggc
     3781 tgcccaacct gaataagaat tcagcattga aactaccacc ggcatatccg ccccaccaat
     3841 agaagaaact aagtgccagc cgaagactaa cgcaataatt gtcataatca gcacagggaa
     3901 gatattgtgc ggattattta aaaacacgat catcaaaaaa acagaaacga taatggctgc
     3961 caaattccat ttgtgtttat gtggaatatt cagtgctgca gaagagactt tacgaccaaa
     4021 taatttaccg cttagtttac caaaggcaac caatgaaccg gagaaggtta ccgcaccaat
     4081 gaaaatacct aagaacactt ccacattgtg gatattggca agagtggctt gttccgctaa
     4141 aaatgccgct tgtgcagttt catctaaatt tgcaggcatt aatgcattgg tatgaagtcc
     4201 gtagctgtta aaaccaacca ataccgctgc taaaccgaca aagctgtgta gaattgccac
     4261 taattcaggc atttcggtca tttctacttt tagggctttt ttcactccga tgaatccacc
     4321 gattgccatt gcgattaaga tccaaatcgt gccatgtgat tgcgggccga aaatggttgc
     4381 cactaaagca atcgccatac ccacaatccc ataccaacaa cctgctttgg cggtttcgtg
     4441 tttagaaaga ccggctaagc tcataataaa gagaatagct gcaataatgt acgcagcagt
     4501 cacaaatcca aaagacatag tttcgtttct ccttaacctt ttctaaacat tgcaagcata
     4561 cgttgcgtta ctttaaagcc accaaaaata ttgatgctgg caaccaaaat cgcaataaat
     4621 gccaaaatat caataaagaa attaccggtc ggttgggcaa tttgtaatac cgcacctacg
     4681 atgataatcc ctgaaatcgc atttgttacc gccattaatg gggtatgtag tgcgtggctg
     4741 acgttccaaa ccacataata ccctaccaca caagcgagaa cgaatacggt aaaatgcgat
     4801 aaaaacgctg ccggagccac agatgcaagc cataggaaca gcacgcctgc tgctgccatc
     4861 acgccatatt tcactcgagg gtctgcaggt ttttcctctt ttttgaccgc ttgtggtgcg
     4921 gctgcttgtt tttgcggttg ggcagatact tgaatcggcg gagccggcca agttaattcg
     4981 ccgtcacgca ccaccgttac gccacgcaac actacatcat caaaattaat ctcaatttga
     5041 ccatctttat ttggtgctaa cagtttgagt aaattcacca agttagtacc ataaagttgc
     5101 gaagattgcg ttggtaaacg tgcaggaaaa tcggtatagc caatcacttt cacttgatta
     5161 tcggtcacca caatctcgcc ggctttggtg tattcacagt taccaccggt tgcagcggct
     5221 aaatcaacaa tcactgaacc cggtttcatt gaatcaacca tctctttagt gattaaacga
     5281 ggagcaggtt tgcccggaat ggcagcggtg gtaataataa tatccacctc tttggcttgc
     5341 tcggcatata acgccattgc acgacggtta aactcttccg acatcacttt cgcatagccg
     5401 tcacctgaac caccttcttc ttcaaaatca atttcgagga aatcagcccc cattgattta
     5461 acttgctctt ttacttcagg gcgagagtca aaggcacgca caatcgcccc caggctattt
     5521 gccgccccga ttgcagccaa gccagcaaca ccggctccaa ttaccaacac ttttgccggt
     5581 ggtactttac ctgctgcggt aatttgccca gtaaagaagc taccaaaagc gtttgccgct
     5641 tcgatcaccg cacggtaacc cgaaatattt gccatcgaac ttaaagcatc gagagcttgg
     5701 gcacgggaaa tacgaggcac agcgtccatt gccaacacat taattttctt agactgcaat
     5761 ttttccatca attgtggatt ttgtgccggc caaataaagc tgactaaggt taccccttct
     5821 ttgattaact caatttcagc ttctgtcggc gcattcactt taaaaataat gtctgcattc
     5881 caaacttctt gttgtgtgcc gatatttgca cctgcattta aaaatgcatt atcttcaaaa
     5941 ctggctttaa aacctgcgtc ttgttccaca agtacgtcaa agccaagttt tttgatctgc
     6001 tcaacggttt ttggcgttgc cgccactcga gtttcaccgt ctaacagctc tcttggtaca
     6061 ccaataagca taaagactcc tgtatggttg ttgtgattaa aaacgctcta aatgtaccac
     6121 taaataaaac aaataggtaa aaaattatgc ccaattaaac cgattatttc ccattttttc
     6181 aaattttaag caggctaaat ttttgctaca ctattaacag gctaaattta acaaaaaaga
     6241 aacaaaacca atgcagattc tcaaccaaat tcagatcatt cttgttgaaa cttcactccc
     6301 ggcaaatatc ggttccgcag cccgagcaat gaaaacgatg ggcttaaccc atttgcgatt
     6361 agtcgcccca aaacaaccgc ttgatgaaca agccgaagcc cttgctgctg gggcaaaaga
     6421 tgtgttggat aacgctcaat gctacgccac tttcgatgaa gccattgccg attgccaact
     6481 ggtgatcggc accagtgcca gacttcgcca tttgcaaaat accctgattg aaccaagaga
     6541 ctgtggtgaa ttagtggtaa atcgagccaa tcttggcaaa gtggcgattg tatttggacg
     6601 tgagcgagtg gggctaacca acgaagaatt attgaaatgc cagtaccatt tgaatttccc
     6661 aaccaatcct gattatggtt ctctgaattt ggcaatggca gtacagctcg caagttatga
     6721 aatccgaatg gcttatttgc aaaattctgg cgaaaaacaa ccgcttgcag cagataagga
     6781 ttacccaacc gctcaagcgt tagagcattt ctttgctcac accgaacgct tatataccga
     6841 attaggcttt atccgcaatg attcagtgat gctaaaactc cgccgtttat accaacgtgc
     6901 cgaactggaa actaatgagc tgaatttact gaggggaatg ctcacggcgg tggagaaggc
     6961 aaattgacaa gcggttaatt tttagtagaa atttgcaaaa tccctgacta aaacaaaaac
     7021 ggtaagatta ctcttaccgt tttctctaaa tcattatttt aaattcaaca gtttatttga
     7081 actttcaata atactgccca ttgtagaatc gccttttaat tgttcttcgc tcatcggttc
     7141 gatatgccaa atatttttcg cataatccat caccgagcgg tctgaggaga aatagcccat
     7201 attggcaata ttgatgatgg ctgaacgtgt ccaagctttc ttgttacgat aggcttgagc
     7261 cactttttct tgagcctcca catagctacg ataatcggca caggcttggt agtagtcacc
     7321 tgaatttaag atcaaatctt gatagcgata cggatcttcc ggtgagaatt taccgtttag
     7381 aatttgtgaa attgcctcat tcaattcgct gtctttttcg tagtagtgat acggtgagta
     7441 accgttgcgg cgaagctctt ccacctgctc tacttcatta ccgaaaataa agatgttctc
     7501 tttaccaacg tgatctaaaa tttcgacatt cgcaccgtct aatgtaccaa tggttaaagc
     7561 accattgagg gcaaatttca tattagaggt tccggaggcc tctgtacccg ctaaggaaat
     7621 ttgctctgaa acatcagctg ccggaataat catttgtgct aatgaaacac cgtagttcgg
     7681 aataaacacc accttaatta aatcattaat gcgagggtca ttattcacca catttgccac
     7741 atcattaatc aaacgaatca cttttttcgc cgcataataa gcacttgccg ctttccctgc
     7801 gaagataaac acacgaggtg tccaatcagc agtcgggttt ttcaagatgc ggttataaag
     7861 cgtgatgata tgcaacacgt tcatttgctg gcgtttatac ttgtgaatac gcttaacttg
     7921 tacatcaaaa atcgcttcag gattaagttt aattccctga gtttcttcga catatttggc
     7981 taagcgacgt ttgttttcta ctttcaccgc tgccacctgt gcttgcacat cagcatcatc
     8041 aacaaacacg ttgaattttt ccaattcact taaatcagtc agccacttat tgccgataca
     8101 tttatctaaa atattagcaa gccctgggtt ggcaattttg atccagcggc gaggtgtcac
     8161 cccattagtc acattagtaa aacgatttgg gtaaatacgg gcaaaatcgg caaaaataga
     8221 ttcaaccatt aaatccgagt gaattttcgc cacaccattt actttcgctg aggcgactac
     8281 cgcaagccac gccatacgca cacggcgatc gccttgttca tcaattaagg aaacacgaga
     8341 aatcaaatcg tgatcttccg ggaattgact acgcacttcc tctaagaaat attcgttaat
     8401 gtcgaaaata atctctaaat ggcgtggtag cacacgggct aacatttcta ccggccaagt
     8461 ttctaacgct tcactcatca aagtgtgatt ggtgtagaag aaaattttac gagtcatcgc
     8521 ccacgcttgt tcccagccat agcctttttc gtcaattaaa atacgcatta actcaggcac
     8581 cgcaagggtt gggtgtgtgt catttaagtg aattgccact ttgtcggcta aattcacaca
     8641 agagccgaac tccgcttcgt ggcggcggat aatatcttgt acagatgccg agcaaaggaa
     8701 atactcttgg cgtaagcgta attcacgacc gttataggta gaatcgtccg gataaagcac
     8761 acgagagaca ttttccgagc tgttttgttt aaccattgct gcaaaataat cgccacggtt
     8821 aaaatcagat aagccaaacc ctctacccga tgcgtgtgca gaccataaac ataagctgtt
     8881 agttgaggtg gtctcaaagc ccgggatcag ttggtcgtga gcttgagcgg taatttcttc
     8941 ttccggctgc catactactt ttttgccctc acgccaagtt ttaccgccaa agcgtaccgg
     9001 gaaacgctta cttgaacgta gatatggcca cacaaattca ttctctaacc attcatcagg
     9061 ttgctcaatt tgctccccgt tgcggatttc ttggcggaac ataccgtatt catattgaat
     9121 accgtagccg attgccggaa ttttcattgt ggcaaggctg tccatatagc aggcggctaa
     9181 acgccctaag ccaccgttac ctaagcccgg atcgccctct tcattgataa tctcttccag
     9241 atcttgccct aactctttta acgcagcgcc aataagctca tacacacctt cagaaatcat
     9301 tgcgttactg aacgtacgcc ccattaaaaa ttccattgaa aggtaataaa cacggcggct
     9361 accatttgca acctggctac ggcgggtttg taaccaatct tcggtagata aatcacgcac
     9421 tacacgtaag gttgcattga gccagttacg cttactggca tcacggggtt cgacccctaa
     9481 agcgaacacc attttgtgca aaatcgcatt tttaacattt ttgatcattt ctgggcttaa
     9541 tgactgcctg ctcattgagt aaattctcct aatttttaat attgcaagcg gtcgttttta
     9601 caaaaaaatc cgcaaattcg accgcttgaa atgatgttgt gaatacaaat taaataattt
     9661 cgtggtataa attttggtac tgttctgcca cttttgccca accaaaatct tgagcaaggg
     9721 cattctggcg aacgctcgac cacaattttt gtttctgcca taagctgatt gcccgcatag
     9781 ctgcctctaa aaacgcaggt gcatctgggt aatcaaatac aaatccggtg gcactgcggt
     9841 tttcaatgct ctctttatgg ctatctacca ccgtatcagc taagccaccg gtacggcgaa
     9901 ccagcggtaa agtgccatat ttcagcccat aaagttgagt taatccgcac ggctcaaaac
     9961 ggcttggtac tagaatcaga tcaccacctg caataatttg gtgcgataaa gcctcgtcat
    10021 aaccgatttt caccccaatt tggttcgggt atttgtgttg gaaataacgt agcgcatttt
    10081 ctaaatctgg cgatccactt cccaatacaa taatctgtac cttatgttgc attaaggtat
    10141 ctatattggt gagtaagaaa tctgccccct tctgctcagt taaacgagtc accattacca
    10201 tcaataatgc ttctttgtct tctggcaaac caaaaattcg ttggagttcc gctttatttt
    10261 tggctttacc gtgcatatag ttcgcacgat aattcaccac aatatgtgta tccgtttccg
    10321 gattccacac ctcagcatcg accccgttta aaatgccacg caatctacct tgatattgac
    10381 gggttgccag taaaccatgc aagcctgctc cggcaatttc ctcagtaatt tcacgagcat
    10441 aggtcgggct aacggtagta atgcgatcag caaaatacaa acctgctttg aggtaagaaa
    10501 tttcaccgta aaattctaag ccttcggcgt ggaaaaattc ccaaggtaag ccaagctctg
    10561 ctaaatgaaa ggcattaaag cgcccgatat aggcaatatt atgaatagtg aagagcgttt
    10621 ttacattgct tccccaactt ttaagatagg cacaagcaag ccctgcttgc caatcgtgtg
    10681 cgtgtaaaac ttgagcggtt ccccaccaat gatccaaacc ttgggctaat tgcgccccaa
    10741 tgtaacctaa taaagcaaag cgtttatagt tatccggata gtcttgataa ttgctgtcgt
    10801 gataagggtt accatcacgc ataaataaat gaggcgcatc gataacgtaa acgcctaagc
    10861 cattaaaata agcaaatcga acagagactg ttccggcaaa agtgccgata gtagcaactt
    10921 ctaccgtttc ccctaaagtt tgcgcgactt tcgggtaata aggaattaac actctgacat
    10981 cattaccagc ttgctgttgc gcaaatggca atgcccccat tacatcagct aaaccgcctg
    11041 ttttaattaa agggtacatt tcagagcaaa tatgtaaaac tttcattttt aaccgttttc
    11101 tatgtgtaaa atagttgaaa aataagaccg cttgaagcgc tttaatcata acaatacttc
    11161 gagcttgcga cctcaaatga caaactcgaa gcattatatc atctcttact aaattgtata
    11221 tttaatatcc gaattggctt cttttcccgc taatttatct aacattgact gtgtcactaa
    11281 tacaatgcca tttttgctga cacgaaaacg tttcgcatct tcttcggcat ttacaccaat
    11341 ttctaagcca tcaggaatta cacaatgtct gtcaatcaca gcgcgcttaa ttacgcaatt
    11401 tttaccgat
//
LOCUS       DANJWC010000074        11309 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.074, whole genome shotgun sequence.
ACCESSION   DANJWC010000074 DANJWC010000000
VERSION     DANJWC010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11309
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.074"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..789)
                     /locus_tag="PWX02_001392"
     CDS             complement(<1..789)
                     /locus_tag="PWX02_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL1729401.1"
                     /translation="MSYKVVFSLLFSLFLSTANANWTKINDVNYTWGPFNIYHITLFT
                     ESGTYVEGTRPLMLTLQYKKPWEGRDFAVSLARTWNNLGIKLPEKELDEVITHLRKTF
                     PDIKPEDTLHYIALEDRGYFILNDKVVSDVFNKAFNDAIVAIWLDPKMDISHQLLDPS
                     YKPRVESKNIESQAEEKSAEVKAQEPIANTEVSKEMKESQTVEQESNKTETLEVDKTQ
                     SSVANEATQPVENKDKKEPEKLEEKPKEMKIENPEVFISPIYDDI"
     gene            979..1194
                     /locus_tag="PWX02_001393"
     CDS             979..1194
                     /locus_tag="PWX02_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HDL1729402.1"
                     /translation="MFPEFRDLITQLKQNNSHFARLFDKHNELDQRIKNMEANIELAT
                     YEEVEALKKEKLHLKDQLYAILKQESA"
     gene            complement(1253..2410)
                     /locus_tag="PWX02_001394"
     CDS             complement(1253..2410)
                     /locus_tag="PWX02_001394"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDL1729403.1"
                     /translation="MSLDLADIRTQITNLDRNLLKLLAERHRLAFDVVRSKEITQKPL
                     RDLEREKILLQELVNFAEAENYQLDPQYVTQIFQRIIEDSVLTQQNYLQNKLNAQKEE
                     QVSIAFLGMRGSYSNMASRQFAKKYQGTLAELSCHSFNEVFEKVSLGEADFGVLPLEN
                     TTSGSINEVYDLLQHTDLTLVGELAYPIKHCVLANGTVDLAEVDTIYTHPQPAQQCSQ
                     FLAGLDKVHIKYCESSSHAMQMVARLNKPNIVALGNEDGGKLYGLTNIKTDIANQAHN
                     ITRFIVVAKEAVQVSPQVQTKTLLLMTTTQQAGALVDALMVFKNHHIRMMKLESRPIY
                     GKPWEEMFYVELEANIHSENTQKALAELEQVTSYLKVLGCYPSEIIEPVKL"
     gene            complement(2532..4598)
                     /gene="prlC"
                     /locus_tag="PWX02_001395"
     CDS             complement(2532..4598)
                     /gene="prlC"
                     /locus_tag="PWX02_001395"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HDL1729404.1"
                     /translation="MSNPLLNYTGLPAFSQIKPEHIKPAVEAVIKECRETVEKVAQIE
                     NPTWENFYMPQAMAGDKFSRAWSPVGHLNAVKNSPELREAYQACLPLLSEYSTWAGQH
                     QGLYQGYVKLKNSPEFETYSVAQKKAIENSLQDFELSGISLPAEKQKRYGEISARLAE
                     LNSQFSNNVLDATMGWDIVITDEAQLKGLPESALEAAKLSAESKGKKGYRFTLEIPSY
                     LPVMTYCENKALREEMYRAYVTRASDQGPNAGKWDNSAVIDEILKLRHEKAQLLGFKT
                     YSDYSLATKMAENPQQVLDFLNDLATRSKAQGKNELCDLKKFAKAHFGIEHLDLWDIP
                     FYSEKQKQALYSVNDEELRLYFPENRVLSGLFEVIKRIFGMRVEEQKEVDVWHKDVRF
                     FNIFDKNDRLRGSFYLDLYARENKRGGAWMDDCINQKRLADGTLQKPVAYLTCNFNKP
                     IGDKPALFTHDEVTTLFHEFGHGIHHMLTEIDVGDVAGINGVPWDAVELPSQFLENWC
                     WEEEALAFISGHYETGEPLPKAKLEQLLAAKNFQAAMFVLRQLEFGLFDFRLHTEYDP
                     SKQGQVLALLKEVKDQVAVAKGVEWARTPHSFSHIFAGGYAAGYYSYLWAEVLSADAY
                     SRFEEEGIFNPETGQSFLDNILTRGGSEEPMVLFERFRGRKPTLDALLRHKGILGNAE
                     PVDCTQ"
     gene            complement(4706..5446)
                     /gene="pflA"
                     /locus_tag="PWX02_001396"
     CDS             complement(4706..5446)
                     /gene="pflA"
                     /locus_tag="PWX02_001396"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HDL1729405.1"
                     /translation="MSVVGRIHSFESCGTVDGPGIRFILFLQGCLMRCKYCHNRDTWD
                     LDGGKEITVEELMKEVTTYKHFMKATGGGVTASGGEAVLQMEFVRDWFRACKAEGINT
                     CLDTNGFVRHYSPVVDEMLEVTDLVLLDLKQLNDEVHQDLVGVSNKRTLDFARYLHKL
                     NKPTWVRYVVVPGYTDDDDSAHRLGQFIQGMDNIEKVELLPYHRLGAHKWETLGYKYE
                     LEGVMPPSKEDLERIQKIIESYGHTVKF"
     gene            complement(5695..6720)
                     /locus_tag="PWX02_001397"
     CDS             complement(5695..6720)
                     /locus_tag="PWX02_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HDL1729406.1"
                     /translation="MSKQDLIRSSVAEYLTFITATGESQVDAVYADENVWLSQKMMAV
                     LYDVDVRTISYHLKKIFSDNELAENSVIQFFWITAQDGKNYHTKHYNLSAIIAVGYKV
                     NSERAVQFRKWATEIVQSFAIKGFAMDDERLKNDGTILGKKYFEEQLARIREIRLSER
                     KFYQKITDIYATAVDYDRTATATKRFFATVQNKLHWAIHGHTAAELIMARANAEKPNM
                     GLTNWKDAPQGKIYPFDVVVAKNYLSDNELAQLQRLVSAYLDMAEDMAERQIPMTMAD
                     WETRLNRFLAATDREILQDAGKVTAEIAKSFALSEFEKYRVKQDLTYESDFDLLVKEV
                     AAKYHAG"
     gene            complement(6955..9279)
                     /gene="pflB"
                     /locus_tag="PWX02_001398"
     CDS             complement(6955..9279)
                     /gene="pflB"
                     /locus_tag="PWX02_001398"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDL1729407.1"
                     /translation="MTQLTDAQQKAWEGFAAGEWQTEVNVRDFIQKNYTPYEGDESFL
                     AEVTPATSKLWADVMEMIKVENKTHEPYDIDCDTPSTITSHAPGYIDKSLEKIVGLQT
                     DAPLKRAIMPFGGINMVKGSCKVYRRELKPEVEQIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVITGLPDAYGRGRIIGDYRRMALYGADFLMKDKQRQFASLQARLESGEDIQATI
                     QLREEIAEQHRALGKMKEMAASYGFDISGPATNAQEAVQWTYFAYLAAVKSQNGAAMS
                     FGRVSSFLDIYIERDLRAGKITEQEAQELIDHLVMKLRMVRFLRTPEYDQLFSGDPMW
                     ATETLAGMGLDGRTLVTKNSFRILHTLYTMGPSPEPNLTILWSEQLPEAFKRYCAKVS
                     IDTSSVQYENDDLMRPDFQNDDYAIACCVSPMIVGKMMQFFGARANLAKTLLYAINGG
                     VDEKSGDQVGPKTEAITSEYLDYDDVMTRLDSFMDWLATQYVTALNIIHFMHDKYAYE
                     AALMALHDRDVFRTMACGIAGLSVAADSLSAIKYAKVKPIRTDIEIKNKAGEVVGVAK
                     NVATDFEIEGEYPQFGNNDNRVDDIAVDLVERFMKKIQKLKTYRNATPTQSVLTITSN
                     VVYGKKTGNTPDGRRSGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTF
                     SIVPNALGKDYEAQKRNLAGLMDGYFHHEATVEGGQHLNVNVLNRDTLLDAVEHPEKY
                     PQLTIRVSGYAVRFNSLTKEQQMDVITRTFTESM"
     gene            complement(9369..10193)
                     /gene="focA"
                     /locus_tag="PWX02_001399"
     CDS             complement(9369..10193)
                     /gene="focA"
                     /locus_tag="PWX02_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HDL1729408.1"
                     /translation="MFSPAEMAKIAEDGAVYKATKNQLYSFLSAITAGGFIAIAFVFY
                     TTTQVGASQMPWGMAKLIGGLVFSLGVIMCVVFGAELFTSSTLTIVARASQRINWLQM
                     FKNWVVVYFGNFVGAISIVLLIWFSGQIMAAKGQWGLVILNTAQHKIHHQWFEAFCLG
                     IFCNIMVCIAVWMSYAGKSLTDKAFIMMLPIAMFVASGFEHCVANMFMIPMGIAISHF
                     AGPEFWTAINVDQTQFADLDLYHFVFKNLIPATLGNIVGGVFFIGLMQWFLYIRKH"
     gene            10394..10888
                     /locus_tag="PWX02_001400"
     CDS             10394..10888
                     /locus_tag="PWX02_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDL1729409.1"
                     /translation="MTEYLTSFFSLFFSEQNQLFTMFLSGFLSATLLPGNSEIIFTTL
                     ASQHLITYGSLSSFSLFNLLLVATLGNSLGSITTYAIGLLLPRPQNTTNRYSQWALAK
                     SEKYGVLVLLFSWSPIVGDIFCGIAGWLRFSLWQSILFIVIGKLLRYLFLLATLYSVL
                     PHIL"
     gene            complement(10942..>11309)
                     /locus_tag="PWX02_001401"
     CDS             complement(10942..>11309)
                     /locus_tag="PWX02_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HDL1729410.1"
                     /translation="IFLARIISTMAASSRDENGNSSTNTLVFWVVDIVLQMVFGVLAT
                     MIAMWFSRQREFRADAGSAQLVGKEKMIAALQRLQRVHEPQEMEGSLAAFMINGSRGK
                     ELFMSHPPLEKRIEALRNL"
BASE COUNT         3205 a         2520 c         2144 g         3440 t
ORIGIN      
        1 gatatcatca taaatcggtg aaatgaacac ttccggattt tcaatcttca tctcctttgg
       61 tttttcttct agcttttctg gctctttctt atctttattc tctaccggtt gagtcgcttc
      121 atttgccaca ctggattggg tcttatctac ctctagggtt tctgttttgt ttgattcttg
      181 ctctacagtt tgagactctt tcatctcctt agaaacctca gtattggcaa taggttcttg
      241 tgctttcact tccgctgatt tttcctcagc ttgtgattct atattttttg actcaactct
      301 tggcttataa gagggatcaa gtaattgatg gctaatatcc attttcggat caagccaaat
      361 tgctacaatg gcatcattga aagctttatt aaacacatca gagactactt tatcatttaa
      421 aataaaataa cctctatctt ctaatgcaat atagtgcaat gtatcttcag gcttaatatc
      481 tgggaaagtt tttctcaggt gggtaataac ctcatctagc tctttttcag gtagcttaat
      541 gcctaaatta ttccaagttc ttgctaaaga aaccgcaaaa tcgcgccctt cccacggttt
      601 tttatattgt agagtaagca ttaaaggacg tgtaccttct acataagtac cgctctcagt
      661 aaaaagcgta atatggtaaa tattaaatgg cccccacgta taattcacat cattaatttt
      721 tgtccaattt gcatttgctg ttgataaaaa tagagaaaac aacagcgaaa agaccacttt
      781 atagctcatt atttttgtgt ccttaaatct aaaattccta ctcattatta tagccttaaa
      841 tatggctcac acacattata attttcgata taaattttgt gatcaagttc aaaaaaatag
      901 aaagatttaa cagtaaatca acaaaaaaat gctagtattt aatcaagtaa aatttgcaac
      961 cactattagg agttaaatat gtttccagaa tttcgtgatc tcataaccca attaaagcaa
     1021 aacaattcac attttgctcg cttatttgat aaacataatg agctagacca gcgtattaag
     1081 aatatggagg caaatattga actggcaaca tatgaagaag ttgaggcact aaaaaaagaa
     1141 aaattgcatt taaaagacca actttatgca attctaaaac aagaatcggc ttaacctaaa
     1201 gtaaaagctc agaaaatatt ctgagcttta atttcggtca tattagacaa tattacagtt
     1261 ttaccggctc aataatttcg cttgggtagc aacccaacac tttgaggtag ctggtaactt
     1321 gctccaattc cgccaatgct ttttgggtgt tctcggaatg aatatttgcc tccagctcca
     1381 cataaaacat ctcttcccaa ggtttgccgt aaatcgggcg ggattccagt ttcatcatac
     1441 gaatatgatg atttttgaat accattaacg catcgaccaa tgcacccgcc tgttgcgtgg
     1501 tggtcattaa cagtaacgtt ttggtttgta cctgtggcga aacctgcaca gcctctttgg
     1561 caacaacaat aaaacgggtg atattgtgag cttgattagc aatatcggtt ttgatattcg
     1621 tcaagccata caattttccg ccatcttcat tgcccaaggc aacaatattc ggcttattca
     1681 agcgagccac catttgcatt gcgtgcgagc tgctttcgca atatttgata tgtactttat
     1741 ctagcccggc taaaaattgg ctacattgct gggcaggctg agggtgggtg taaatggtat
     1801 ccacctccgc caaatcaacc gtgccgtttg ccaatacaca atgtttgatt gggtacgcca
     1861 attcacccac taaggttaaa tcggtatgct gcaataaatc atacacttca ttgatcgaac
     1921 ctgaagtcgt gttttcaagc ggcagtacgc caaaatcggc ttcaccaagg ctgactttct
     1981 caaatacttc attaaaagaa tggcagctta attccgccaa tgtgccttga tattttttgg
     2041 caaactgtcg agatgccata ttggaataag agcctcgcat tcccaaaaac gcaatcgaaa
     2101 cctgctcttc tttttgtgca ttcaatttat tttgcagata attttgctga gtcagcactg
     2161 aatcttcaat aatacgttgg aaaatttggg tcacatattg cggatcaagc tggtaattct
     2221 ccgcttcggc aaaatttacc agttcttgaa gcaaaatctt ttcccgttct aaatctctca
     2281 agggtttttg tgtgatttct ttgctccgca ccacatcaaa ggctaagcgg tgacgttctg
     2341 ccagtagctt taataaattg cggtctaagt tggttatttg ggttcgaata tcggctaaat
     2401 ctaaagacat tatttcactc tctgtcaggc taaatgctga aaattataac gagttgcaaa
     2461 agaaagaaaa gacaagcggt caaatttaca aaaaaatttg caaatctaac cgcttgtaaa
     2521 acgtgtttac tttattgagt gcaatcaaca ggctcggcat tacctaaaat gcctttgtgt
     2581 ctgagcaagg catcaagggt tggcttacgc ccacggaagc gttcgaacag caccattggc
     2641 tcttctgaac cgcctcgggt taagatatta tctaagaaag actgccccgt ttcagggttg
     2701 aaaatcccct cttcctcaaa gcgtgaatag gcatctgctg aaagcacttc cgcccataaa
     2761 taactgtagt agcccgccgc atagccgcct gcaaaaatat gcgagaaact gtgtggcgtt
     2821 ctcgcccact ctacgccttt ggcaacagcg acttggtctt tcacctcttt aagtaaggct
     2881 aacacttgtc cttgtttgct cgggtcatat tcggtgtgta ggcggaaatc aaacaagcca
     2941 aattctaatt ggcgaagcac aaacattgcc gcttggaagt ttttcgccgc caataattgc
     3001 tccaatttcg cttttggtaa cggctcgccg gtttcataat gccctgaaat aaaggctaat
     3061 gcctcttctt cccagcacca gttttctaag aattggcttg gcaactccac tgcgtcccac
     3121 ggcacgccgt taataccggc aacatcgccc acatcaattt cagtcagcat atggtgaatg
     3181 ccgtgtccga attcatggaa caaggtagtc acttcatcgt gagtaaacaa tgccggttta
     3241 tcgccaatcg gtttattgaa gttacaggtt aaatatgcca ccggtttttg taatgtgccg
     3301 tcagccaagc gtttttggtt gatgcaatca tccatccacg ccccaccacg tttgttttca
     3361 cgagcgtata aatctaagta gaacgagcca cgcaagcggt catttttatc aaaaatattg
     3421 aaaaaacgta cgtctttgtg ccatacatca acctcttttt gttcttccac acgcataccg
     3481 aaaatgcgtt taatcacctc aaataagccc gataatacac gattttccgg gaaatacagg
     3541 cgaagttctt catcattgac tgaatacaat gcctgttttt gtttttcgct gtagaacgga
     3601 atatcccata aatctaaatg ttcaatgccg aaatgggctt tagcaaattt tttcaaatcg
     3661 cataattcat ttttgccttg agctttcgag cgagtggcta aatcgttcaa gaaatccagc
     3721 acctgttgtg gattttccgc cattttggtg gctaatgaat aatcagaata ggtcttaaag
     3781 ccgagtagtt gggctttttc gtgacgcagt tttaagatct catcaatcac cgcactatta
     3841 tcccatttgc ctgcattcgg gccttgatca gaggcacgag tcacataagc acggtacatt
     3901 tcctcacgca atgctttgtt ttcacaataa gtcatcaccg gcaaataact tgggatttca
     3961 agagtgaagc gatagccttt cttacctttg ctttctgcgg ataattttgc cgcttctaat
     4021 gccgattccg gcaagccttt aagctgtgct tcatcggtaa tcacaatatc ccagcccatt
     4081 gttgcatcaa gtacgttgtt gctaaattgc gagttgagtt ccgccaaacg agctgaaatt
     4141 tcgccgtagc gtttttgctt ctctgccggc agtgaaatgc ctgataattc aaaatcttgc
     4201 aagctgtttt caatcgcctt tttctgtgca accgaataag tttcaaactc agggctgttt
     4261 tttaacttca catagccttg atataaccct tgatgttgcc ccgcccaagt gctgtattcc
     4321 gataataacg gcaagcacgc ttggtaagcc tcacgcaact ctggggaatt tttcaccgca
     4381 tttaaatgcc cgaccggtga ccacgcacgg gagaatttat ccccagccat tgcttgtggc
     4441 atatagaaat tttcccacgt cgggttttca atttgtgcga ctttttcaac agtttcacgg
     4501 cattctttga tcaccgcttc taccgccggt ttaatatgtt caggtttgat ttgtgagaac
     4561 gcaggcaagc ccgtgtaatt taaaagaggg ttagacatat taatgttcct ttttagatgt
     4621 aggggcgaaa tatttttcgc ccttttaaaa ataatatgac aagggcgaaa aatatttcgc
     4681 ccctacaatt caggatttta caaaattaaa atttcacggt atgcccgtaa ctttcaatga
     4741 ttttttggat acgctctaaa tcctctttag acggtggcat taccccttcc agctcatatt
     4801 tatagcctaa agtttcccat ttatgagctc ctaaacgatg ataaggcaga agttccactt
     4861 tctcaatgtt atccattcct tgaataaact gccctaaacg atgggcagaa tcatcgtcat
     4921 cggtataacc cggcacgacc acatagcgaa cccaagtcgg tttatttaat ttgtgtagat
     4981 aacgggcgaa atcaagggta cgcttattcg acaccccgac taaatcttgg tgaacttcat
     5041 cattgagctg ttttaaatcc agcaagacca aatcggtcac ttcaagcatt tcatccacca
     5101 ccgggctata atggcgaaca aaaccattgg tatctaagca ggtattaatg ccttcagcct
     5161 tacaggcacg aaaccaatcc cgcacaaact ccatttgcaa caccgcttcc ccacccgaag
     5221 ctgttacacc accgccggtt gctttcataa aatgcttata agtcgtgact tctttcatca
     5281 gctcttcaac cgtaatttcc ttaccaccat caagatccca agtatcacga ttatggcaat
     5341 atttacaccg catcaaacag ccttgcaaga acagaataaa acgaatcccc ggtccatcta
     5401 ccgtaccgca ggattcaaaa gaatggatac gtccaacaac agacatttgt cacctcaacc
     5461 ctcaaaaaat tttgagaaat tgtagcaaat ttttatgttg ttcgcagaaa taaatcacac
     5521 cggtatattt taagaaagga gaaagtcata ctattttttg cctatttcag gaataggctt
     5581 ttattaatct attgactcca tcaacatatc aaccaaatat gtttaaattt ttgcattttt
     5641 taaagctatt ttgaccgctt acaagcagtc ggtttttctg aaaactttgc aaacctagcc
     5701 ggcgtggtat ttggcggcga cctcttttac cagtaagtcg aaatcgcttt cgtaggtgag
     5761 atcctgcttg actcggtatt tttcaaattc gctgagggcg aaagatttag caatttcagc
     5821 ggtaactttg ccggcatctt gtaagatttc tcggtcggtg gcggcaagaa agcggttaag
     5881 gcgggtttcc cagtctgcca ttgtcatcgg aatttggcgt tctgccatat cttccgccat
     5941 atccaaatag gcagagacca gccgttgtag ttgtgctaat tcattatccg ataaataatt
     6001 tttggcaacc accacatcaa aggggtaaat tttgccttgc ggagcgtctt tccaattcgt
     6061 tagccccata ttcggctttt cagcgttggc tcgtgccata atcagctcgg cggcggtatg
     6121 cccgtgaatt gcccaatgga gcttgttctg cacggtggca aaaaagcgtt tggtggcggt
     6181 tgcggtgcgg tcgtagtcca cggcggtagc gtaaatgtcg gtgatttttt ggtagaactt
     6241 gcgttcggat aaccgaattt cccgaatacg agcaagctgt tcttcaaagt attttttgcc
     6301 caaaatcgtg ccgtcatttt tcagccgttc atcgtccatc gcaaagcctt taatcgcaaa
     6361 gctctgcacg atttcggttg cccatttgcg aaactgcacc gctcgctcgg aattgacttt
     6421 ataacccacc gcaataattg ccgacagatt ataatgcttg gtgtggtagt ttttgccgtc
     6481 ttgggcagtt atccaaaaaa attggataac tgaattttca gctaactcat tgtcactaaa
     6541 gatttttttg agatgatagc taatggttcg tacatcaaca tcatacaaca ccgccatcat
     6601 tttttggcta agccatacat tttcatcggc gtaaacggca tcaacttggc tttcgccggt
     6661 agctgtaata aaagtgaggt attccgccac agaggaacga attaaatctt gcttagacat
     6721 ctctttctcc catatagaac actgtaatta taaacaatat tacgaaagct gtgtaaatga
     6781 atagccaatt tttcgagcga gatcacaaaa aaaccgcttg cggcataaat gctacaagcg
     6841 gttgattatt actcacaaaa ttgtttccaa ttttgttcgc tcctacggag ccgtcggcaa
     6901 agccaacgtt caaaacgcaa tgcgttttgt ggctaaaagt tttgcaaatt gtaattacat
     6961 actttcagtg aaggtgcggg taattacgtc catttgttgc tctttggtta aagagttgaa
     7021 acgtaccgca tagcctgaaa cacgaatcgt taattgcgga tatttttcag ggtgttctac
     7081 cgcatctaat aatgtatcac ggtttaacac gttcacattt aagtgctgac cgccttctac
     7141 agttgcttcg tggtggaagt agccgtccat taagcctgca aggttacgtt tttgggcttc
     7201 gtaatcttta cctaacgcat ttggtacgat agagaaagta taagaaatac catctttcgc
     7261 ataggcaaac ggtaatttcg ccacagaagt taatgaagct accgcacctt tctggtcacg
     7321 accgtgcatt gggttcgcac ccggtccgaa tggagcacct gagcgacgac cgtccggtgt
     7381 attaccggtt ttcttaccat ataccacgtt agaagtgata gtaagcacag actgagtcgg
     7441 cgttgcattg cggtatgttt tgagtttttg aattttcttc ataaaacgtt caactaaatc
     7501 aactgcaatg tcgtccacac ggttatcgtt gttaccgaat tgcggatatt caccctcaat
     7561 ttcaaagtcg gttgctacgt ttttcgctac accaaccact tcgccagctt tgtttttgat
     7621 ttcaatatcg gtacggattg gttttacttt cgcatattta atggctgaaa gtgagtctgc
     7681 cgctactgaa agccctgcga taccacacgc cattgtgcgg aacacatcac ggtcgtgaag
     7741 agccattaat gccgcttcgt atgcatattt atcgtgcata aagtggataa tgttcagtgc
     7801 tgtcacatat tgggttgcaa gccaatccat aaagctgtct aagcgggtca ttacatcatc
     7861 gtaatctaag tattcacttg taattgcttc agttttaggc cctacttggt cgcctgattt
     7921 ctcatcaaca ccgccattga ttgcgtataa caaggttttc gctaagtttg cacgagcgcc
     7981 gaagaactgc atcattttac ccacgatcat tggcgatacg cagcacgcaa tcgcatagtc
     8041 atcgttttgg aaatccggac gcattaagtc atcgttttca tattgaactg atgaagtatc
     8101 aatagacact ttcgcacagt agcgtttaaa tgcttccggt aattgttcag accaaagaat
     8161 agttaagttt ggctccggtg acggtcccat tgtgtaaagg gtatgtaaaa tacggaagct
     8221 gtttttggtt actaatgtgc gaccgtctaa acccataccg gctaaggttt cggttgccca
     8281 cattggatcg cctgagaata attgatcgta ttctggagta cgcaagaaac gcaccatacg
     8341 caatttcatt actaagtggt cgattaattc ttgggcttct tgctcggtaa ttttaccggc
     8401 tcttaagtca cgttcgatat aaatatctaa gaaagaagac acacgaccaa aagacatcgc
     8461 cgcaccgttt tgcgatttta ccgctgcaag gtaagcgaaa taagtccatt gcaccgcttc
     8521 ttgtgcattg gttgccggac ctgaaatatc aaaaccgtag cttgctgcca tctctttcat
     8581 tttgcctaaa gcacgatgtt gctcggcaat ttcttcacgc agttgaatcg tggcttgaat
     8641 atcttcgccg gattctaaac gagcttgtaa tgaggcaaac tggcgttgtt tgtctttcat
     8701 taagaaatcc gcaccgtaaa gtgccatacg gcggtagtca ccgataatac gaccacgacc
     8761 ataagcatcc ggtaaaccgg taattacgcc cgatttacgg caacgtaaaa tatccggagt
     8821 gtaaacatcg aatacccctt ggttatgagt tttacgatat tcagtaaaga tttgttctac
     8881 ttcaggttta agctcacggc gataaacttt gcacgaacct tttaccatat tgataccgcc
     8941 aaacggcata atggcacgtt ttaatggggc atctgtttgc agaccgacga ttttttctaa
     9001 cgatttatca atataacccg gtgcgtgtga ggtaatggtt gatggcgtgt cgcagtcaat
     9061 atcatacggc tcgtgtgttt tgttttctac tttgatcatt tccatcacat ctgcccacaa
     9121 tttgctggtt gcaggtgtta cttcagctag gaaagactca tcaccctcat aaggtgtgta
     9181 gtttttttgg ataaaatcac gtacatttac ttcagtttgc cactcacctg cggcaaaacc
     9241 ttcccacgct ttttgttgag cgtctgttag ttgagtcatt ttaagtcctc tgtgttagta
     9301 agttaaaacg ggaaagcggt aattttttgc aaaaaatttg caaaatttga ccgcttgatt
     9361 ctgaaatctt agtgctttct aatgtataag aaccactgca ttaagccgat gaaaaacaca
     9421 ccgccgacaa tattacccag cgttgccgga attaggttct taaacacaaa atgatataga
     9481 tctaaatcgg caaattgcgt ttggtcaaca ttgattgccg tccaaaattc aggaccggca
     9541 aagtgagaaa tcgcaattcc catcggaatc ataaacatat ttgccacaca gtgttcaaag
     9601 ccggaagcca caaacattgc aatcggtaac atcataataa aggctttatc ggttaggctt
     9661 ttacctgcat aagacatcca caccgcaatg cacaccataa tgttacagaa aatgcctaaa
     9721 cagaaagcct caaaccattg gtggtgaatt ttgtgctgtg cggtatttaa aataaccaaa
     9781 ccccattgcc ctttggctgc cataatctga ccactaaacc aaatcaacaa tacaatggaa
     9841 atcgccccaa caaagttacc gaaatacacc actacccagt ttttaaacat ctgtaaccag
     9901 ttaattcttt ggctggctct ggctacaatc gtaagtgttg atgaggtaaa aagttctgcc
     9961 ccaaacacca cacacataat cacgccgagt gaaaatacca aaccaccaat cagtttagcc
    10021 ataccccaag gcatttggct cgcacccact tgagttgtgg tataaaatac aaatgctatt
    10081 gcaataaaac cgcccgcagt aatggcagag agaaaggaat ataattgatt ttttgttgct
    10141 ttataaaccg caccatcttc tgcgattttt gccatttcag caggagaaaa catcagacaa
    10201 ccttaacaat gagaattgaa aaaccgatat attatgtaga aattatagac ctcttgagat
    10261 aatcttcaaa taaaaatatt acacaatcag aggtatttcg atgtcttttt ttgatataga
    10321 acaaaatcca taaaaaacac aacattcaag aaaatttata actacatttt aataacaaac
    10381 caactacaat tttatgacag aatatctcac ttcctttttc agtttattct tttccgaaca
    10441 aaatcaactc tttactatgt ttctgagtgg atttctaagc gctaccctct tacccggaaa
    10501 ttctgaaatt atcttcacta ccttagcaag ccaacatctc atcacttatg gctctttatc
    10561 ctcattttca ctgttcaatt tactcttggt tgcaacctta ggtaatagtt taggtagcat
    10621 aaccacttat gcgatagggc tattactccc tcgcccacag aatactacca atcgttattc
    10681 acaatgggca ttagccaaaa gtgaaaaata tggcgtgtta gtcttgcttt ttagttggtc
    10741 accgattgta ggcgatattt tctgtggcat tgccggctgg ctacggttta gcctttggca
    10801 aagcattctc tttattgtga ttgggaaatt gttacgttat ctgtttttgc tggcaactct
    10861 ctattcggtg ttaccgcata ttttatagaa aacaaaagcg gctgttttgc aaaaaatttt
    10921 acaaaaacaa ccgcttgtgc tttataagtt tcttaacgct tcaatacgtt tttctaacgg
    10981 agggtggctc ataaacaact ctttaccacg gctaccgtta atcataaagg cagcaagaga
    11041 gccttccatt tcttgcggtt cgtgaacacg ttgcaagcgt tgtaatgccg caatcatttt
    11101 ctctttaccg actaattgag cagagcctgc atctgcacga aattcacgtt ggcgagagaa
    11161 ccacattgca atcattgttg ctaacacacc aaacaccatc tgtaacacaa tatctactac
    11221 ccagaacacc agcgtattgg tgcttgaatt gccattttcg tcacgtgaac ttgccgccat
    11281 tgtcgaaata atacgagcta agaaaatga
//
LOCUS       DANJWC010000075        11254 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.075, whole genome shotgun sequence.
ACCESSION   DANJWC010000075 DANJWC010000000
VERSION     DANJWC010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11254
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.075"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            85..744
                     /locus_tag="PWX02_001402"
     CDS             85..744
                     /locus_tag="PWX02_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL1729411.1"
                     /translation="MFNISKQQVLDAFHFRSACRSYDPNKKINKEDMDYILELGRLSP
                     SSVGSEPWKFLVLQNPETRQKIAPVSWGIKHPMEEMSHLVVILTKKNVRYDSVYMRDM
                     LAKRGLTYEQIEASLVRYKSFQEEDMKLLSERELFDWSTKQTYIALGNMMTGAALIGI
                     DSCPIEGFHYDTVNQILSDEGLFDLNEYGVSCMITFGYRNKEIKKKSRKPTEEVIAWI
                     E"
     gene            complement(804..2128)
                     /locus_tag="PWX02_001403"
                     /pseudo
     CDS             complement(804..2128)
                     /locus_tag="PWX02_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249339.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
     gene            complement(2229..2981)
                     /locus_tag="PWX02_001404"
     CDS             complement(2229..2981)
                     /locus_tag="PWX02_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon-containing protein"
                     /protein_id="HDL1729412.1"
                     /translation="MYLAIVHFSYHNFEQDPVTAISQVLNQWLYNGQVIGREMPITFH
                     QNEFQARVCVPTQESLLPQYNSDEVNEALQQAVEIGVNFASFELVGRDYQGEESSHNR
                     SPAFQILYTTHLDTCSPLYDGEQFAPIPLYSLQDQSLSEAILEWQQNWQACDLLQMNG
                     NVLECHALAQISDETSVLATIGRDLCQQIEQRTHIPTFYYLYRLGRDEGEYQRKCPCC
                     GGEWKLTEPLHDIFHFRCDPCRLISNLSWELL"
     gene            complement(2981..3880)
                     /locus_tag="PWX02_001405"
     CDS             complement(2981..3880)
                     /locus_tag="PWX02_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HDL1729413.1"
                     /translation="MNEKYSKLVKSAANLAIIVAILLIVIKAFAWWKTGAISILAAMT
                     DSVVDLFASLTNILVLRFALQPADENHAFGHGKAESLAALAQSAFISGSALFLLLQGF
                     HKLTNPELIAQSDIGVIVSIASIVITALLVFYQRYVVTLTKSPAIEADSLHYQTDLLM
                     NAAILVAMLLNSAGVVYADAVFAIGIALYIAFNAVKMFWCAVQTLLDQALPDEEIEQI
                     KQIASQHPNILGIHDILTRRAGAVRFIQLHLELDDHLTLLVAHEITDSLEQKLLAAFP
                     MSHVIIHQEPTTVVKQEFMAENI"
     gene            complement(3889..4452)
                     /locus_tag="PWX02_001406"
     CDS             complement(3889..4452)
                     /locus_tag="PWX02_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATPase"
                     /protein_id="HDL1729414.1"
                     /translation="MSATKKGITLVALSLAGCVSQSPQFAVPDAIRFGQDTFVKVTHS
                     QIDEMQHLLYLPEKSEKNLENWQKGILFFLDKNSKNQTLEQRAAFRQASFAKKPELEA
                     KVAIEQNELHSSVIYPPTERFNNVMLEVTRGRNLACGYGQIQFADKREVSKAIAKKLP
                     NLTAYSQEIAKLSLELNQLGWQVQCSK"
     gene            complement(4452..5228)
                     /gene="yaaA"
                     /locus_tag="PWX02_001407"
     CDS             complement(4452..5228)
                     /gene="yaaA"
                     /locus_tag="PWX02_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDL1729415.1"
                     /translation="MLAIISPAKTLDFKTNIDGFAFSQPNLTACSQELIDICKQLSPA
                     EVGSLMSISDKLAALNVARFAEWQLEHNQQNSKAAIFAFKGDVYTGLDAETLTKEQVE
                     YAQQHLRMLSGLYGLLKPLDLMQPYRLEMGTKLANPKGRDLYAFWGNIITEHLQQAID
                     EQGDDILINLASDEYYGAVKPQNLNATIIKPVFLDEKNGKYKVISFYAKKARGMMVRF
                     MLDTQPTSIEQLKQFNYGGYWLDADSSSENELVFKREEQA"
     gene            complement(5328..5723)
                     /gene="rpsI"
                     /locus_tag="PWX02_001408"
     CDS             complement(5328..5723)
                     /gene="rpsI"
                     /locus_tag="PWX02_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL1729416.1"
                     /translation="MTAANQNYGTGRRKSSSARVFIKPGSGNITINQRSLEVYFGRET
                     ARMIVRQPLELVELTDKLDLYITVKGGGISGQAGAIRHGITRALMEYDETLRPALRAA
                     GFVTRDARRVERKKVGLHKARRRPQYSKR"
     gene            complement(5740..6168)
                     /gene="rplM"
                     /locus_tag="PWX02_001409"
     CDS             complement(5740..6168)
                     /gene="rplM"
                     /locus_tag="PWX02_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDL1729417.1"
                     /translation="MKTFVAKPETVQRDWYVVDATGKTLGRLATELARRLRGKHKPEY
                     TPHVDTGDYIIVINADKVAVTGKKQTDKIYYWHTGYVGGIKDATFKEMIARRPEAVIE
                     IAVKGMLPKGPLGREMFRKLKVYAGSEHNHQAQQPQVLDI"
     gene            6467..6862
                     /locus_tag="PWX02_001410"
     CDS             6467..6862
                     /locus_tag="PWX02_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcB family protein"
                     /protein_id="HDL1729418.1"
                     /translation="MEQWTTDVWAAIMAAFIVGAVIGCTLVRVFNSSVRKQHELEKEL
                     KQTKTQVDEQKQKLEQHFEQSAQLLSSLASDYKKLYTHLAQSSEKLLPEASKNITFFQ
                     QTKLPIPAEKTEEDQPRDYSEGSSGLLKS"
     gene            complement(7023..8594)
                     /gene="guaA"
                     /locus_tag="PWX02_001411"
     CDS             complement(7023..8594)
                     /gene="guaA"
                     /locus_tag="PWX02_001411"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL1729419.1"
                     /translation="MTNIHHHKILILDFGSQYTQLIARRVREIGVYCELWAWDVTEEQ
                     IREFNPTGIILSGGPESTTEENSPRAPEYVFNAGLPVLGICYGMQTMAMQLGGLTETS
                     DHREFGYASVAIKSADQLFANLNDDLTACEPKLDVWMSHGDKVTKLPPHFTITGVTPT
                     CPIAAMSDESRRFYGVQFHPEVTHTKQGLALLKNFVVGICGCETNWTAENIIEDAVKR
                     IREQVGDDEVILGLSGGVDSSVTALLLHRAIGKNLHCVFVDNGLLRLNEGDQVMEMFG
                     NKFGLNIVRVNAEDRFLEALKGLDEPEAKRKTIGKVFVDVFDDESKKLSSVKWLAQGT
                     IYPDVIESAASKTGKAHVIKSHHNVGGLPDYMKLGLVEPLRELFKDEVRKIGLALGLP
                     AEMLNRHPFPGPGLGVRVLGEIKKEYCDLLRKVDAIFIEELHKAGWYYKVSQAFTVFL
                     PVKSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDLLGKISNRIINEVNGISRV
                     VYDVSGKPPATIEWE"
     gene            complement(8611..8922)
                     /locus_tag="PWX02_001412"
     CDS             complement(8611..8922)
                     /locus_tag="PWX02_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729420.1"
                     /translation="MKKLSLISIVALSACSIFGPSYSGETTASPLLKLDTERNINLYF
                     QAIHKCTPDKIHTKIINIPSEGNAKELWTATGCNKTQSFIITYRNDGVGGTFINMTTT
                     P"
     gene            complement(9044..9715)
                     /locus_tag="PWX02_001413"
     CDS             complement(9044..9715)
                     /locus_tag="PWX02_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL1729421.1"
                     /translation="MLQAIIFDMDGVIVDTEYLEFSLQKQFIEDIKEHSRPITLEQQS
                     EVVGKCLKEIPVIIKKLSESSLPIEEIRARYYAFFQDLFSKVDFKTIFRADIQQIIQF
                     AKQNQIKLAVASSSALSHIENILTVCGIKDEFDLIVSGEQFEHSKPDPTIYRYTCEKL
                     GVEPQNAVAIEDSYYGMLAAKTAGLTVIGYEEKRILIDQSLADYMGKDMKEILKILQK
                     CNTPS"
     gene            complement(9836..>11254)
                     /gene="guaB"
                     /locus_tag="PWX02_001414"
     CDS             complement(9836..>11254)
                     /gene="guaB"
                     /locus_tag="PWX02_001414"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDL1729422.1"
                     /translation="LVPAHSTVLPNTADLSTQLTKTIRLNIPMLSAAMDTVTETKLAI
                     SLAQEGGIGFIHKNMSIERQADRVRKVKKFESGIVSEPVTISPDMTLAELAELVKKNG
                     FAGYPVIDENQNLVGIITGRDTRFVTDLSKTVREFMTPKDRLVTVKENASREEIFHLM
                     HEHRVEKVLVVNNEFQLKGMITLKDYQKAESKPNACKDEFGRLRVGAAVGAGPGNEER
                     IDALVKAGVDVLLIDSSHGHSEGVLQRVRETRAKYPDLPIVAGNIATAEGAIALADAG
                     ASAVKVGIGPGSICTTRIVTGVGVPQITAIAEAAAALKDRGIPVIADGGIRYSGDISK
                     AIAAGASCVMVGSMFAGTEEAPGEIELYQGRAFKSYRGMGSLGAMSKGSSDRYFQSDN
                     AADKLVPEGIEGRIAYKGYLKEIIHQQMGGLRSCMGLTGCATIEELRTKAEFVRISGA
                     GIKESHVHDVTITKEAPNYRMG"
BASE COUNT         3242 a         2516 c         2224 g         3272 t
ORIGIN      
        1 gcaaattaac aggcgtgaaa ttcgcattct gctctaaatc aagttatcat taatctactt
       61 atttcactta taaaaggaaa aacgatgttt aatattagca aacagcaagt tttagacgct
      121 tttcacttcc gttcggcctg tcgcagttac gatccaaaca aaaaaatcaa caaagaagat
      181 atggattata ttttggaact cgggcgtctc tcaccaagct ctgtcggttc tgaaccgtgg
      241 aaatttttag tgctacaaaa cccggaaacc cgtcagaaaa tcgccccggt ttcgtgggga
      301 attaaacacc caatggaaga aatgagccat ttagtggtga ttttaaccaa aaagaatgtc
      361 cgttatgatt ctgtctatat gcgtgatatg ttagccaaac gaggcttaac ctatgagcag
      421 attgaagcct cgctggtacg ctataagtcg ttccaagaag aggatatgaa attgcttagt
      481 gaacgtgaat tattcgattg gagtaccaag caaacctata ttgcattagg caatatgatg
      541 accggtgcgg ctctgattgg tattgactct tgcccgattg aaggttttca ttacgatacc
      601 gtaaaccaaa ttttatcgga cgaaggctta tttgacctaa acgaatatgg tgtgtcttgt
      661 atgataactt ttggctatcg caacaaagaa attaagaaaa aatcccgcaa acctaccgaa
      721 gaagtgattg cgtggattga ataatcacct taacttataa aaacgggcga attaatcgcc
      781 catttttata tctttagttt tgactactcg gctttcaccc atttattggt taatgtgccc
      841 gccaccattg caccattcac gtttaaggcg gtgcgagcca tatcaataat cggctcaact
      901 gaaatgagca agcctactaa ctcaatcggc agattcaagg ttgatagcac cacaatcgcc
      961 gcaaaagttg caccaccacc tacaccggca atgccaaatg aggaaattgc caccaccaaa
     1021 atcaaggtta aaatatagtt taagataaac ggatccattc ccactgttgg agcgaccatc
     1081 accgccaaca ttgccggata aattcccgca cagccatttt gcccgatggt tgccccaaag
     1141 gttgcagaaa agttggcaat cacactgtta ttacctaatt attggtttgc gtctcaatat
     1201 tcaacggaat ggttgccgca ctggaacgag acgtaaaagc aaaacttaat gtcggcaaca
     1261 cttttttgta gtaatccaac gggttgattt tagccacaaa gagtaaaata ccgtgcacaa
     1321 taaacatcaa accaatcgca atgtatgatg ccacaataaa gctacctaaa tttacaatat
     1381 cgctccattg agaagttgcc gccattttga tcatcaacgc aaatacgcca taaggggtta
     1441 ggcgaatcac aaaacgtact aaacgcataa ccagtttatt taaggtctct acaccttgtg
     1501 cgatacgttc gcccaacgct ttatcttctt tgcctaagct cagtgcagca atgcctagca
     1561 gagccgagaa aataaccgtg ttgatgatcg acgttgggtt tgcaccggtt aattctaaaa
     1621 acggattttt cggaatgaaa gaaagcaaca ttgaaggcac agataagttg ctcacctgct
     1681 ccacacgccc taccactttc tcttgagcgg caagctcacg ggagccggcg actaaaccct
     1741 cggcagataa atcaaacaga tacactaaag caataccaat cgctgcggca atcgcggtgg
     1801 tcactaataa cactgatagc acactaaaac taattttgcc taaagatcct gcctctttta
     1861 agcgggtaat tgccgataaa atagagacaa acaccaatgg cattacgatc atttgtagca
     1921 agcgaacata accgttacca attaagttaa tccaatcaag agttgcatca acagtagctt
     1981 tttcgtaaaa agtttgcaaa aaagcaccgc ttgcaagccc taaaaataac ccaataaaca
     2041 ccgtttgacc gagtttttgc gtagatttat aaattttagc caataaaaat agtattgccg
     2101 caaagacaat taagttgaaa ataagcacag aacctccaaa gtaaaaccgc ttattataaa
     2161 aatgccgcaa gagtattacg cttgcgacat tcattcaaga tgatttatta ctttggtata
     2221 actaaaagtt ataataattc ccagcttaga ttagagatca aacggcaagg atcacatcta
     2281 aaatggaaaa tatcatgcaa cggctcagtt aatttccatt ctccgccaca acaagggcat
     2341 ttgcgttgat attcaccttc atcacgccct aaacggtaga ggtaataaaa ggtgggaatg
     2401 tgggttctct gctcaatttg ctgacataga tctcgaccga tcgttgcaag aacgcttgtt
     2461 tcatcgctaa tttgagcaag tgcgtggcac tctagcacat tgccgttcat ttgtagcaga
     2521 tcgcacgctt gccaattttg ctgccattcc aatatcgctt cgctaagaga ctgatcctgt
     2581 aaagaataaa gcggaattgg ggcaaactgc tctccatcat agagtggcga gcaggtatct
     2641 aaatgggtgg tatataatat ttgaaacgct ggcgatcggt tatgactgct ttcttcgccc
     2701 tgataatctc gcccgacaag ctcaaaactt gcaaaattca cgccaatttc aaccgcttgt
     2761 tgcaacgctt cattcacttc atcgctatta tattgtggca aaaggctttc ttgtgtcggc
     2821 acacaaaccc gagcttgaaa ctcattttgg tgaaaggtaa tcggcatttc tctgccaatg
     2881 acttgcccgt tatataacca ctgatttaag acttggctga ttgccgtcac cggatcttgc
     2941 tcaaaattat ggtagctgaa atgcacgatt gccagataca ttaaatattc tccgccataa
     3001 attcttgttt gaccaccgta gtcggctctt gatgaatgat aacgtgagac attggaaatg
     3061 ccgccagcaa tttctgctca aggctatcag ttatttcgtg agctaccagc agcgttaagt
     3121 gatcgtctaa ttccaaatgt agttggataa accgtaccgc acctgcacgg cgggttagaa
     3181 tatcgtgaat ccctaaaatg ttcggatgtt gcgaagcaat ctgttttatc tgttcaattt
     3241 cttcatcggg caaggcttgg tctagcaaag tttgtaccgc acaccaaaac attttcactg
     3301 cattaaaggc aatgtagagg gcaataccaa ttgcaaaaac cgcatcagca tagacaacac
     3361 cggcactatt gagcaacatt gccactaaaa ttgctgcgtt cattaataaa tcagtttggt
     3421 aatgtaatga atccgcctca atcgccggac ttttcgtgag cgtaacgaca taacgctgat
     3481 aaaagactaa aagtgcggtg atcacaatgg aagcaatact cacaattacc ccaatatcag
     3541 attgagcaat taattccgga ttagtcagtt tgtggaagcc ttgtagcagc aggaaaagcg
     3601 ccgagccgga aataaaggca ctttgagcaa gtgccgctaa agattccgct ttgccgtgtc
     3661 cgaaggcgtg attctcgtct gccggttgca acgcaaaacg caacaccaaa atattagtca
     3721 gcgaagcaaa cagatctact accgagtccg tcattgctgc caagattgaa attgcgccgg
     3781 ttttccacca cgcaaaggct ttaatcacaa tcagtaagat cgcaacaata atcgctaaat
     3841 ttgctgcact tttgactaat ttgctgtatt tttcgttcat ttactacatt atttactgca
     3901 ttggacttgc caacccagtt ggtttaattc aagagaaagt ttggcaattt cttggctgta
     3961 agcggtcaaa tttggcaatt tttttgcaat tgccttgcta acttcccgct tatctgcaaa
     4021 ttgaatctgc ccataaccac aagctaaatt ccgcccacgg gttacttcta acatcacatt
     4081 gttaaaacgt tctgtcggtg ggtaaatcac actactgtgt aattcatttt gttcgattgc
     4141 cacttttgct tcaagctccg gctttttcgc aaaacttgct tggcggaatg cagctcgttg
     4201 ctctaaggtt tgatttttac tgtttttatc caagaaaaat aaaatgcctt tttgccaatt
     4261 ttccagattt ttctcactct tttcgggcaa ataaagtaaa tgttgcattt cgtcaatttg
     4321 gctgtgagta acttttacaa aagtatcctg cccaaagcga atagcatcag gcactgcaaa
     4381 ttgtgggctt tggctgacac aacctgccaa tgataacgca accaaagtta ttcccttttt
     4441 ggtagccgac attatgcctg ctcctcacgt ttaaaaacca gctcgttttc actagaacta
     4501 tctgcatcaa gccaatagcc cccatagtta aactgtttga gctgttcgat gctagtcggt
     4561 tgggtgtcta acataaagcg gaccatcatt ccacgggctt ttttggcata gaagctgatc
     4621 actttatatt tgccgttttt ctcatctaaa aacaccggct tgataatagt cgcatttaga
     4681 ttttgtggct taactgcacc ataatattca tcagaggcaa gattaattaa aatatcatcg
     4741 ccttgttcat caatggcttg ctgcaagtgt tcagtaataa tattacccca gaacgcataa
     4801 agatctctgc ccttcggatt tgccaactta gttcccattt ctaaacgata aggctgcatt
     4861 aaatcgagcg gttttaataa accatacaag cctgaaagca tacgtaaatg ttgctgagca
     4921 tattcaacct gctctttagt tagcgtttct gcatctaacc ctgtgtaaac atcgccttta
     4981 aaagcaaaaa tggcggcttt agagttttgt tggttatgct caagctgcca ttcggcaaag
     5041 cgagcgacat ttaaagcggc taatttatcg ctgattgaca tcaaacttcc tacttcggct
     5101 ggtgaaagct gtttacaaat atcaattaat tcttggctac aagcggtcaa atttggttga
     5161 gaaaatgcaa aaccgtcaat attggtttta aaatcaaggg ttttggcagg ggaaataatg
     5221 gctaacataa atgcctctat caaatagtta atcagggcgg ttattctacc acagtcataa
     5281 agacaaaaaa ggcaggaaac cctgccttct tgaaaacgaa aagaaaatta acgttttgag
     5341 tattgtggac gacgacgtgc tttgtgtaaa cccacttttt tacgttcaac gcgacgtgcg
     5401 tcacgagtaa cgaagcctgc tgcacgaagt gctgggcgta gcgtttcatc gtattccatt
     5461 aatgcacgag tgataccgtg acggattgca cccgcttgac cagaaatacc accaccttta
     5521 acagtgatgt aaaggtctaa tttatcagtt aattcaacca attctaacgg ctgacgtacg
     5581 atcatacgag ctgtttcacg accgaagtaa acttcaagtg aacgttggtt gatagtaatg
     5641 ttacctgaac ccggtttgat aaatacacgg gctgaagagc ttttgcggcg acctgtgccg
     5701 tagttttgat ttgctgctgt catttttatg ctccgtgaat tagatatcta aaacttgtgg
     5761 ttgttgtgct tggtgattgt gttctgaacc tgcgtaaact tttaatttac ggaacatttc
     5821 acgaccaagt ggaccttttg gtaacatacc tttcactgcg atttcaatca cagcttcagg
     5881 acgacgagca atcatttctt tgaaggttgc atctttgatg ccacctacat agccagtgtg
     5941 ccagtagtag attttgtcag tttgtttttt acctgttact gctactttgt ctgcattgat
     6001 cacgatgatg taatcaccgg tatcaacgtg tggagtgtac tcaggtttat gtttaccacg
     6061 aagacggcgt gccaattcag tcgctaaacg acctaatgtt ttacccgtcg catctactac
     6121 ataccagtca cgttgtactg tttctggttt tgctacaaaa gttttcatta attaaatacc
     6181 aaatttaaag tttaaaaccc aatacctcat tgaggtattt cctattattt tacggtgaac
     6241 ccgacccctt cgagtctgat tcagaccaaa cttgtgtggc ggggaaaacc acacaaaaac
     6301 agggtggcga aattatacag agatttcact caaaaatcta acattttttg ctgataatct
     6361 ttcaaaattg aacctatatc acattttaat catttggagc ttttcaaata aaaactgaaa
     6421 tgttaaaaat agcagtaatc aatcactaaa acataaggag tttatgatgg aacaatggac
     6481 aacagatgtt tgggcagcca ttatggcagc atttattgta ggtgctgtaa taggttgcac
     6541 tctagttaga gtatttaaca gtagtgtcag aaaacagcat gaactggaaa aagagttgaa
     6601 gcaaaccaaa actcaagtgg atgagcaaaa acaaaaactt gaacaacact ttgagcaatc
     6661 agcccaatta ctttcaagtt tagcttctga ttataaaaag ctttataccc atttagcaca
     6721 aagctctgaa aagctattac cagaagcatc aaaaaatatt acctttttcc aacaaaccaa
     6781 attacctatt ccggcagaaa aaacggaaga agatcaacct agagattatt ccgaaggttc
     6841 atctggctta ctgaaatcat aaaactaaaa attgaatatt tagttttact aaaaggcact
     6901 tagtttaagg attgattccg ttggaatcaa tcctttattc taaaccgcta cattaagcag
     6961 ttaacaacat ttttcagctt tagatactga acttaggtta taagaagcct attttgtaag
     7021 agttattccc actcaatggt tgccggtggt tttccgctca cgtcatacac cacacgggaa
     7081 ataccgttca cttcgttaat aatgcggttg gagattttgc ctaatagatc atacggcaag
     7141 tgcgcccagt gagcggtcat aaagtcgatg gtttccaccg cacgcaagct gacgacccag
     7201 tcatatttgc gtccatcgcc cattacgccc accgatttca ccggcaagaa taccgtgaaa
     7261 gcttggctga ctttgtagta ccagccggct ttgtgcagtt cttcgatgaa gatcgcatcg
     7321 actttacgca gtaaatcgca gtactctttt ttgatttcgc ccagcacacg cacgcctaag
     7381 cccgggccgg ggaacgggtg gcggttgagc atttcagccg gtaagccgag tgccaagccg
     7441 attttgcgca cttcgtcttt aaacaattca cgcaacggtt cgactaagcc gagcttcata
     7501 taatccggca agccgccaac gttatggtgt gatttaatca cgtgggcttt gccggttttg
     7561 ctggcggcgg attcaatcac atcagggtag atcgtgcctt gtgcaagcca tttgaccgag
     7621 ctaagttttt tgctttcgtc atcaaacacg tccacaaaca ctttaccaat ggttttgcgt
     7681 ttagcttccg gttcgtctaa gccttttaac gcttctaaga aacggtcttc ggcattcact
     7741 cgcacaatgt tcaaaccgaa tttgttgccg aacatttcca tgacttgatc gccttcgttc
     7801 aagcggagca agccgttatc cacaaatacg cagtgcaggt ttttaccgat agcacgatgt
     7861 aatagcaagg cggtgacgga ggaatccacc ccacccgaca aacctaaaat cacctcgtca
     7921 tcgcccactt gctcacgaat gcgtttcacc gcatcttcaa tgatgttttc tgccgtccag
     7981 ttcgtttcac agccgcaaat gccgaccacg aagtttttca gcaacgccaa gccttgtttg
     8041 gtgtgggtca cttcggggtg gaactgcacg ccatagaaac ggcggctttc gtccgacatc
     8101 gcggcaatcg ggcaggttgg ggtaacgcct gtgatggtaa agtgcggtgg taatttggtg
     8161 actttgtcgc cgtgactcat ccacacgtcc aattttggct cgcaagcggt caaatcatcg
     8221 tttaaatttg caaaaagttg atcggcagat ttgatagcca ccgaggcata gccaaattca
     8281 cggtggtcgg acgtttcggt taaaccgccc aactgcatcg ccattgtctg cattccgtag
     8341 caaatgccca gcactggcag accagcgtta aacacatact ccggcgctcg tgggctgttt
     8401 tcctcagtgg tgctttcagg acctcctgaa agaataatcc ccgttgggtt aaattcacgg
     8461 atctgctctt ccgttacgtc ccaagcccaa agctcgcagt aaaccccaat ctcccgcaca
     8521 cggcgtgcga taagctgagt atattgtgaa ccgaagtcta aaattaaaat tttgtggtgg
     8581 tggatgtttg tcattgtaat ttcctttaaa ttatggcgtt gtggtcatgt taataaaagt
     8641 gccgcctacc ccatcgtttc tatatgtaat aataaaagat tgcgttttat tacaacctgt
     8701 tgcagtccaa agttcttttg cattaccttc acttggaata ttgatgattt ttgtatgaat
     8761 tttatcagga gtacatttat gtattgcttg aaaatacaga tttatatttc tttctgtgtc
     8821 caactttaat aaaggactgg ccgttgtttc tcctgaataa gatggtccga aaatactgca
     8881 cgcagataaa gcgacgattg aaattaaact taattttttc attctattta tcctattaat
     8941 attaaaatat ccactctcat ttctaggtta ctcaagccga tagactgttc caagtacttg
     9001 aagtagaaac attgacaaaa tttctataaa aatcgaccat ttattaactt ggagtattac
     9061 atttttgaag aatcttcaaa atctctttca tatctttgcc catataatcc gctaaacttt
     9121 ggtcaatcag tattcgtttt tcttcgtaac caatcaccgt taagcctgcg gttttggcgg
     9181 cgagcattcc gtagtaggag tcttcaatcg ccactgcatt ttgtggctca acgcctaatt
     9241 tttcgcaggt gtagcggtaa atggttgggt ctggcttgct gtgttcaaac tgttcaccgc
     9301 tgacgattaa atcaaattcg tctttaatgc cgcaaaccgt gagaatattt tcaatatgcg
     9361 aaagggcgga agaagaggca accgcaagct taatttgatt ctgcttggca aattgaataa
     9421 tttgctgaat atcggctctg aaaatggttt taaaatccac tttgctaaac agatcttgga
     9481 aaaaggcgta gtaacgtgca cgaatttcct caatcggcaa actggattcg cttagttttt
     9541 tgataatcac cggaatttct tttaaacatt tgcctaccac ttccgattgt tgttctaaag
     9601 taatcgggcg gctgtgttct ttgatgtctt caataaactg tttttgtaag gaaaattcca
     9661 gatattcggt atccacaatc acgccgtcca tatcaaaaat gatagcttgg agcatatagc
     9721 ctcttttgta aaattttaag aaaatctaac cacttattcc ctctctctgt tttaaattac
     9781 tgaacgtaat ttaaaacggt ctctctccca caaggggaga gagtgagaaa ggctattaac
     9841 ccattcggta gttcggtgct tctttggtaa ttgtcacatc gtggacgtgg ctttctttaa
     9901 taccagcacc actaatgcgg acaaattctg ctttggtgcg gagttcttca atagtggcac
     9961 agccggttaa tcccatacag gagcgtaaac cgcccatttg ttggtggata atttctttca
    10021 agtagccttt gtaagcgata cgcccttcaa tcccttccgg tacgagcttg tcggcggcat
    10081 tatcagattg gaaatagcga tctgacgagc ctttactcat tgcacctaat gatcccattc
    10141 cacggtagga tttgaatgct ctgccttgat aaagctcaat ttcacccggg gcttcttctg
    10201 tgccggcaaa catcgaaccg accattacgc aacttgcacc ggcggcaata gcttttgaaa
    10261 tatcgcctga ataacgaatt ccaccatcag caatcacagg aatgcctcga tctttaagcg
    10321 cagctgccgc ttctgcgatt gccgtgattt gtggcacgcc aacgcctgtt acaattctgg
    10381 tggtacaaat tgaacccggg ccgattccta ctttcacagc actggctcct gcatcagcta
    10441 acgcaatcgc tccttctgca gtggcaatat taccggcaac aatcggtaaa tcagggtatt
    10501 ttgcacgggt ttcacgcaca cgttgtaata caccttcaga atgcccgtgc gaagagtcga
    10561 ttaatagcac atcgacaccc gcttttacta aagcatcaat tcgttcttca ttgcccggac
    10621 cggctcccac tgccgccccc acacgcaaac gcccaaactc atctttacag gcattcggtt
    10681 tgctttccgc tttttggtag tcttttaggg taatcattcc ttttaactga aattcattat
    10741 tcactaccag cactttctcc actcggtgtt cgtgcattaa gtggaaaatt tcttcacggc
    10801 ttgcgttttc ttttaccgtc actaaacggt cttttggtgt cataaattca cgcactgttt
    10861 tgcttaaatc cgtgacaaat cgggtatcac gtccggtaat aattcccact aaattttggt
    10921 tttcatcaat caccggatag cctgcaaaac cgttcttttt caccaattca gccaattccg
    10981 ctaatgtcat atcaggagaa atcgtcactg gctcagaaac aataccactt tcaaattttt
    11041 tcactttacg cacacggtct gcctggcgtt caatcgacat atttttatgg ataaaaccaa
    11101 tgccgccttc ttgagcaagg gagatcgcaa gcttagtttc tgtaacggta tccattgcag
    11161 cagaaagcat cggaatgttt aaacgaatgg ttttagttaa ttgagtagaa agatcagcag
    11221 tattaggaag cacagtagaa tgtgccggga cgag
//
LOCUS       DANJWC010000076        11205 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.076, whole genome shotgun sequence.
ACCESSION   DANJWC010000076 DANJWC010000000
VERSION     DANJWC010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11205
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.076"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(64..939)
                     /locus_tag="PWX02_001415"
     CDS             complement(64..939)
                     /locus_tag="PWX02_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL1729423.1"
                     /translation="MWIWFTLLAAVAQAGRNAFQKQLSVNVPVLGVTLARFFYAVPLA
                     SGYLYFLYQYDDNLNIPHFPPLFFVYAIGAGLAQILATSLMVQLFHLKNYAIGVGLAK
                     SEAVWAALLGVLFFGVTISGIGWLGVVLGGVAIFLMTGSGNLRQLSWKTLFLGIASGL
                     CFALTSLLVREASLQLNSHYLLSAAWVLFSVISFEAVILVIYLALLQKETLIKFFRFP
                     KLGMLTSLFSFLGSFGWFNAMSLNHVAFVKTLGQVEIFFILGISYFVFKERLKIQDFI
                     GLILVVIAAILVVLG"
     gene            1047..1883
                     /locus_tag="PWX02_001416"
     CDS             1047..1883
                     /locus_tag="PWX02_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDL1729424.1"
                     /translation="MLPHIYIGTGGYADTDLIGTLYPFGTAKEDFLFVYSQHYDTLEI
                     NSTFHAPIGQKAFEGMVQKAEGRLNFSVKLHQDFSHQRTATLDQAQLFLNALAPFTAE
                     GLLANLFVQFPTQFERTPLNRRYLAELCQWFADYPLAIEFRHPSWHIPAVFDTFAKSS
                     NLIWCNVDYPQNIGLPAFHFFANQRTAYLRLHGRNPNWWKGQSAAECHDYRYSDEELK
                     GLAELLFNRRTEFDRLYLYFENTTKSHAFYNIQTLKGFLAEKGFQIKPVPENLLGQQS
                     LF"
     gene            complement(1958..6322)
                     /locus_tag="PWX02_001417"
     CDS             complement(1958..6322)
                     /locus_tag="PWX02_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL1729425.1"
                     /translation="MKHTFKLSLLATSIAISLSPTLLASIVRGDVDYQYFRDLAENKG
                     QFFVGATNIPVIDKTGKNIGTFLQVTPTKQVEVESNISPKPNVSLSECFDNESLPGCY
                     EFLYGGGDEDSNDLPVFETVTGETMSIPMIDFSSITRQTGIATLVNPQYVISVNHNQG
                     YRDIQFGDSSSEKADDHHYNYSVVRRNNYMPDPNKERTDLGKGIEKRERNDEGEKLIL
                     DGSGNPTPRWDYHAPRLSKLVTEVAPANEIERTTENVQDFYSVFSDPFIFPMFIRAGS
                     GRQAVINNERDRLNNGRIEVGNGPFLTGGSVLPVTNGDPSVPGSDFNSRILVAKTENN
                     TINDVFKDHGYGPLTTLGLPGDSGSALFGYDVRTKKWVVLGVYSDYFSENNTPGGDVY
                     KSYWNYHHPHYVRALEKENNAGAINANGARLTWTPSGNTSSIVGGNAPLTVNLADTSL
                     PTLQGHNGNTGDNPWNPQLQHGKTFHILGENNTLSLTENINQGAGAIHFHGNTTVEGM
                     KAGITWLGAGVDIDKDKNVIWKISNPAGDRLSKIGQGTLTINGKGENKGSVSVGDGTV
                     ILNQQADENNKKSAFSELGIVSGRPTVILNSSDQMDPNNIYFGYRGGRLDVNGNDLTF
                     RRIQNSDEGAMVVNHNTAQTANITLTGIVSPTIDQVKVQAITSPDQVSRHLDKDIFTY
                     QRFPHLPTNFIRLKKPLSVAEITAIFNARKVFIDLDFATGLIRENEYFEVLKVTDESA
                     AKQQILAEKIKAAEGTVQGFNGYFGETDPSKTNGRLNITMNSNEVANGKLLITGGTNL
                     NGTLSAEGTSEIILSGRPTPHAYDKVANKEVLIEGDWINRSFNATIFAAKDNGKLEIS
                     RNVANINGNFNLTDNATAQIGFTQGTSQACIRSDRTGVATCNINATLSETDLNSWERT
                     KVAGNVSLADNSTFSLGSKADLTGSITAQESTKVQLNDGSIANLTGLSTTGIFNGANG
                     STVNLSGVWTTLTNTVVDVLNVANGSQIHLNTPTATKQNTLTANTLTGNGIFKFVADL
                     TKTASDIVTIKDTASGQFSIEISTNGSAHSAQKIKLFETTNEEDFSLNLANEVRSGEY
                     RYHLLKEGNVYYLNPEKEEEPKITQKGPSETADPLPEYTGDIEYSEKGPGETADPLPE
                     YTGDIEHSEKGSGETAEPLPKYNGNAHLIASELTAQTNAVLGVTTVLDHVIHSEGTQN
                     WKVWSKTDYQTTEHRSAGYGFKQDANVIHLGLEKALDNQVTLGMILSQSKARNNFDHY
                     YSGKGRLTMLSMYAKKTWQNGVFVAIDTGFGKASNRLTYQANTVKLDRSVFVTGLSIG
                     KAWESANVNIIPSFSARYHHLSSAGNQLVDAKIETNAVDLLALQAGLSINKTLELNGL
                     QIKPEIGSYFVDASHGKLRTRFNNLQIEQQMGRYFKQEAGISANYRNINAGIQAGFLT
                     GNTLNKQRYISFKVTYEW"
     gene            complement(6642..8408)
                     /gene="aspS"
                     /locus_tag="PWX02_001418"
     CDS             complement(6642..8408)
                     /gene="aspS"
                     /locus_tag="PWX02_001418"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDL1729426.1"
                     /translation="MMRSHYCGALNRSHLGQTVTLSGWVHRVRNLGRFIFMQIRDREG
                     IVQVFFDEKDEALFKQASALRSEACVQIQGEVIARDESQINKEMATGEIEVLVKNLVV
                     YNNAEVLPLDFNQNNTEEQRLKYRYLDLRRPEMAEKLKTRAKITSFVRRFMDDNGFLD
                     IETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFR
                     DEDLRADRQPEFTQIDVETSFMTAEEVRALMEAMIHGLWKKTLGVDLGKFPMMTWNEA
                     MTRFGSDKPDLRNPLEMVDVADILKSVDFKVFSGPANDPKGRVVALRVPNGAALTRKN
                     IDDYTQFVGIYGAKGLAWLKVNDMNTGLEGIQSPVAKFLNAEVLKALVKRLNLQKDDI
                     VFFGADNWHVATNAIGALRLKVGNDLGLTDTTAWKPLWVIDFPMFERDDEGNLSAMHH
                     PFTSPKELSPEELVQDPENAVANAYDMVINGYEVGGGSVRIFSQQMQQTVFSILGINE
                     EEQKEKFGFLLDALKFGTPPHAGLAFGLDRLTMLITGTDNIRDVIAFPKTTAAACLMT
                     EAPSYANPKALEELAIRTIPQE"
     gene            complement(8563..9474)
                     /locus_tag="PWX02_001419"
     CDS             complement(8563..9474)
                     /locus_tag="PWX02_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HDL1729427.1"
                     /translation="MLYGLAIVLIPLFLGYLITLKNHRYMAIVNRVVSLCLYLILLVM
                     GISLGQIDNILTELPQIGGTAFSLVVILLLSNIIGLVIYDKLQPMKRDKVNQDQLPSR
                     LHLLIDSFKLIGITLLGGIIGYFTKGVVDFPLHASTYVLEVMIFGVGIQLRNSGIPLR
                     EVFFNKRGIYTSIVMVASSLIGGVISAFTLDLSLAKGLTFASAFGWYSLSSVLVNDAW
                     GPIYGSIAFFNDISREILCLFLVPLFMRQFPSTAVGIGGATSLDCMLPIIQKSGGIQI
                     VPLAISFGFIVNLAAPLLLAVFIGLEG"
     gene            9597..10679
                     /gene="dinB"
                     /locus_tag="PWX02_001420"
     CDS             9597..10679
                     /gene="dinB"
                     /locus_tag="PWX02_001420"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDL1729428.1"
                     /translation="MSIQAKHKRKIIHIDMDCFYAAVEMRENPALVGKPVAVGGNANQ
                     RGVLTTCNYEARKFGLHSAMPTATALKKCPNLILLPVNMALYKLVSAQIHEIFHRYTD
                     VIEALSLDEAYLDVTECERYFGSATWIAQAIRNDIFNELHLTASAGVAPLKFLAKIAS
                     DQNKPNGQCVITPDDVQNFVKNLPLAKIPGVGKVTNEKLKQLGFETCGDIQQAEKAVI
                     YRAFGKFGQRLWDFSHGIDERAVESYRERKSLAVENTLLHDISTLEQAEKIVADLFDK
                     LVFRLQRNWGDTPLTEFKKLGIKLKFDDFSQTTLERTTDGFAYERFGELLQQIFTRRK
                     ERKVRLIGLSVHFPEKKVNKQLNLWE"
     gene            10767..>11205
                     /locus_tag="PWX02_001421"
     CDS             10767..>11205
                     /locus_tag="PWX02_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HDL1729429.1"
                     /translation="MAKRVILFLLTNLAITFVLGIVLNLIFKVTGIQGQSTAGILVLS
                     LVFGFSGSLISLFMSKSMALRSVGAEVITQPRNEAEQWLFNTVQRQAQQANIPMPDIA
                     IYHSADVNAFATGATKSNSLVAVSTGLLNSMTKDEAEAVVAHEV"
BASE COUNT         3242 a         2317 c         2292 g         3354 t
ORIGIN      
        1 tattggtttt gcgtgggctt atgcccaccc tacaaagcta gataaaaaac gtaaaaatcc
       61 gatctagccc agcaccacca agattgcggc aatcactact aaaatcagcc ctataaaatc
      121 ttgaatttta agccgttctt taaacacaaa ataggaaata ccgagaataa agaaaatttc
      181 cacttgtcca agcgttttta caaaggcaac gtggttcagg ctcattgcat tgaaccaacc
      241 gaatgagccg agaaatgaaa acaggctggt caacatacct aattttggaa aacgaaagaa
      301 tttaatcagt gtctcttttt ggagaagagc taggtatata accaatatca ctgcctcaaa
      361 gctaatcacg ctaaaaagta cccacgccgc tgaaagcaaa tagtggctgt tgagttgcag
      421 gcttgcctct cgcaccaaaa gtgatgtgag ggcgaaacat aaaccgctgg ctatgccgag
      481 aaagagtgtt ttccacgaaa gttggcgtag attaccgctg ccggtcatta agaaaatcgc
      541 cactccacct agtaccacgc caagccagcc aatgccactg atggttacac caaaaaataa
      601 cacgcccagt aaagccgccc atactgcctc gcttttcgcc agcccgacac ctattgcata
      661 attttttaag tgaaaaagtt gcaccattag cgaagttgcc agaatttgag ccaacccagc
      721 cccgatagca tatacaaaaa acagcggtgg aaaatgggga atgttgaggt tatcgtcata
      781 ttggtataaa aaatagagat aaccgcttgc caacggcaca gcatagaaaa agcgggcgag
      841 cgttaccccc aatacaggca cattgacgct taattgcttt tgaaaggcat tgcgaccggc
      901 ttgagccacg gctgccagca gagtaaacca aatccacata atgatgatga aataagaaaa
      961 gatagccaaa tcttactctt ttattctcag tttgcaagcg gttaaaaaac cattaaaatt
     1021 tgcaaaaaat tgaaagaata aacacaatgc tccctcatat ttatattggc accggcggct
     1081 atgccgacac agatttaatc ggcacgcttt acccattcgg cactgcaaaa gaggattttc
     1141 tctttgtcta tagccaacat tacgacacgc tggaaattaa cagcacattc cacgccccca
     1201 tcgggcagaa agcctttgaa ggaatggtgc agaaagcgga aggtcggctg aatttttcgg
     1261 tgaaactgca tcaagatttc agccatcaac gcacggcgac tttagatcaa gcacaattat
     1321 ttcttaatgc gttagctccg ttcaccgctg aagggttatt ggcaaattta tttgttcagt
     1381 tcccaactca atttgaacgt acgccactta accgccgtta ccttgccgag ttgtgccaat
     1441 ggtttgcgga ttatccgtta gcgattgaat ttcgccaccc aagctggcat attccggcgg
     1501 tgtttgatac ctttgcgaaa agctctaatc tgatttggtg taatgtggat tacccacaaa
     1561 atatcggctt accggcattt cacttttttg ccaaccaacg tactgcttat ttacgcttac
     1621 atggtcgtaa cccgaattgg tggaaagggc agagtgcggc agaatgccac gattaccgct
     1681 attcagacga ggaattaaaa ggcttggcag aattgctttt taaccgcaga acggaatttg
     1741 acaggctcta tctctacttt gaaaacacca ctaaaagcca cgccttttat aatattcaaa
     1801 ccctaaaagg ctttttggcg gaaaaaggat ttcaaatcaa acctgtgccg gagaatttgc
     1861 ttgggcagca gagtttgttt tgatgggtaa tttacattgt tgaggagtaa ataaagggct
     1921 atgccattta gtaatggata gcccttaatc tcaatattta ccattcatat gtaactttga
     1981 acgagatata acgctgttta tttagcgtat tccccgttaa gaaacctgcc tgaattcctg
     2041 cgtttatatt gcgataatta gcggatatac ctgcttcttg tttaaaataa cgccccattt
     2101 gctgctctat ttgtaaatta ttgaaacgtg ttcttaattt cccatggctt gcatcaacaa
     2161 aatagctacc tatttccggt tttatttgca ggccatttag ttcaagcgtt ttattaattg
     2221 acagaccagc ttgaagagcg agaagatcaa cagcgtttgt ttcgattttt gcatctacaa
     2281 gttgattacc tgctgaagat aaatgatgat aacgggcgct aaatgatggg attatattca
     2341 cgtttgcaga ttcccatgct ttaccgatac taagccctgt tacaaatact gaacgatcta
     2401 attttactgt atttgcttga taagttaagc gattgcttgc tttcccaaaa ccggtatcta
     2461 tcgcaacaaa aacaccattt tgccatgttt tcttcgcata cattgatagc attgttaagc
     2521 ggcctttacc gctataataa tggtcaaaat tatttctcgc ttttgattga gataatatca
     2581 taccaagtgt gacctgatta tctaaggctt tctctaatcc tagatggata acgttcgcat
     2641 cttgtttaaa tccatatccg gcagagcgat gctctgtcgt ttgataatct gttttagacc
     2701 atactttcca attttgggtc ccttccgaat gaattacatg atctaatact gtagttacac
     2761 ctaatacagc attagtttgc gcagttaact cggatgcgat caaatgtgca tttccattgt
     2821 atttaggtaa tggctcagcg gtttcacccg atcctttttc tgaatgctca atgtctcctg
     2881 tgtattcagg taacggatca gcagtttcac ccggcccttt ttcagaatac tcaatatccc
     2941 cggtgtattc aggtaatggg tcagcggttt cacttggccc tttttgagta attttaggtt
     3001 cttcctcttt ctctggattg aggtaataca cattcccttc tttgagtaaa tggtagcgat
     3061 attcgcctga gcgcacttca tttgctaaat taagagaaaa gtcttcttca tttgttgttt
     3121 caaataattt aatcttttgg gctgaatggg cgctaccatt tgtgctaatt tctatcgaaa
     3181 attgaccgct tgcggtgtct ttaattgtga ctatatcgct cgctgttttc gtcagatcag
     3241 cgacaaattt aaatatgccg ttccctgtga gggtattagc ggtaagcgta ttctgttttg
     3301 ttgcagttgg agtgtttaaa tggatttggc taccatttgc gacattcaag acatctacaa
     3361 cagtgttggt taaggttgtc cataccccag aaagattaac ggttgaacca ttggcgccat
     3421 taaatattcc tgttgtactc aagccggtta aattagcaat acttccatca ttaagttgaa
     3481 ccttagtgga ctcctgagct gtaattgagc cagttaagtc tgctttactg cctaacgaaa
     3541 atgttgaatt gtcagctaaa gagacattgc cagccacttt ggttctctcc cagctgttta
     3601 aatcagtctc tgataaagta gcgttaatat tacaggttgc tacgcctgtg cggtcagagc
     3661 gaatacatgc ttgtgaggta ccttgggtaa aaccaatttg agcggtagca ttatctgtta
     3721 aattaaaatt accgttaata ttggcaacat tacggctgat ttcaagcttg ccattgtctt
     3781 tagctgcaaa gattgttgcg ttgaacgatc ggttgatcca gtcgccttca atcaggactt
     3841 ctttattcgc tactttatca taggcatgag gtgttggacg gcccgataaa atgatttcgc
     3901 ttgtaccttc tgcagaaagc gtaccgttga ggttcgttcc cccagtgata agcaatttgc
     3961 cgtttgctac ttcattactg ttcattgtaa tgtttaatcg accattggtt ttgctaggat
     4021 cagtttcacc aaaataaccg ttaaaacctt ggacggtgcc ttctgctgct ttgatttttt
     4081 cagcaagtat ttgttgcttt gcagcacttt catctgttac cttgagtact tcaaagtatt
     4141 cgttttcacg aatgagacca gtagcaaagt ctaaatcgat gaaaacttta cgcgcattaa
     4201 aaatagctgt gatctctgca acgcttaaag gttttttcaa acggataaaa ttagttggca
     4261 agtgtggaaa acgttgatat gtgaaaatat ctttgtccaa atgacgagaa acctgatctg
     4321 gactagtaat tgcttgaact ttcacttggt caattgttgg actaactatg ccggttagtg
     4381 taatgtttgc tgtttgggct gtattatgat tgacaaccat cgctccttca tcgctattct
     4441 gaatacggcg gaacgtgaga tcattgccgt tgacatctaa gcgcccacca cgataaccaa
     4501 aatagatatt gttaggatcc atttgatctg atgaattgag gattacggta ggtctgccac
     4561 tcacaatacc tagctctgaa aatgcagatt ttttgttatt ttcatccgct tgttgattta
     4621 atataacagt gccgtcacca acgctaacgg agcctttgtt ttctcctttg ccgttgatag
     4681 ttagtgtccc ttgtccgatt tttgataatc tgtcaccagc agggttactg attttccaga
     4741 tcacattttt atctttatca atatcaacac cggctcctaa ccaagtaatg cctgctttca
     4801 taccttccac agtggtatta ccatgaaaat ggattgctcc tgcaccttgg ttgatattct
     4861 ctgtgagact tagcgtattg ttttcaccca aaatatggaa tgttttaccg tgttggagtt
     4921 gaggattcca agggttatcg cctgtattac cattatgacc ttgaagtgtt ggtaatgaag
     4981 tatctgctaa attaactgta agaggagcat tccctcctac gattgagctg gtattgccag
     5041 aaggtgtcca agtgagtcta gcaccatttg cattaatagc gcctgcatta ttttcttttt
     5101 ctaaagcacg tacataatgt gggtggtgat agttccagta agatttataa acatctcccc
     5161 caggcgtatt attctctgag aaatagtcac tatatacacc taacacaacc cattttttgg
     5221 tacgaacatc ataaccaaaa agagcggagc cactatcgcc aggtaaaccg agagttgtta
     5281 agggaccgta gccgtggtct ttaaatacat cgtttatagt attattttca gtttttgcta
     5341 ctaaaatacg gctgttaaaa tcgctgcctg gaacgcttgg atcgccatta gtaacaggca
     5401 gaacgctacc tcctgtcaaa aaaggaccgt taccgacttc aatacgcccg ttgtttaagc
     5461 gatctctttc gttgttaatc actgcttgtc taccagaacc ggcacgaata aacatgggga
     5521 aaataaaagg gtcgctaaat acactataaa aatcttgtac attttcggta gtgcgttcaa
     5581 tctcattagc tggagcgact tcagttacta atttcgataa cctaggtgcg tggtagtccc
     5641 agcggggcgt tgggttacct gaaccatcta aaatcagttt ttctccttcg tcattacgtt
     5701 cacgtttttc tatgcctttc ccgagatctg ttctttcttt atttggatca ggcatatagt
     5761 tattacggcg caccacacta taattgtaat gatggtcgtc ggctttttcg ctagatgaat
     5821 caccaaattg aatgtcccga tagccttggt tgtggtttac actaataacg tattgtggat
     5881 taactaatgt tgcaatccct gtttgacgag taatggaaga gaagtcaatc attggaatag
     5941 acatagtctc acctgttact gtttcaaaaa caggtagatc attagagtct tcatcacctc
     6001 caccatagag aaattcataa caaccgggta aactttcatt atcaaagcat tcagataggc
     6061 ttacattcgg cttaggtgaa atatttgatt ccacttcaac ctgcttagtt ggtgtaacct
     6121 gtaaaaaagt accgatgttt ttgcccgttt tatcaataac tggaatattg gttgccccaa
     6181 caaaaaattg gcctttattt tctgctaaat cacgaaaata ttgataatcg acatcaccac
     6241 gtacaataga tgccagtaat gttggtgaaa gagaaatagc aattgatgtt gctagaagag
     6301 agagcttgaa agtatgcttc attattattt gaaacctgta taaatatgta agtagatcag
     6361 atttgagcgc tattaaaaag attatttaaa tcgattaaat attcatttaa ataccacaca
     6421 attattcagc gctattatct tgttcggcat aaatctgctc aatacccaaa taaggtaata
     6481 attaaaataa taggggatat ttagtagtaa catagagaag agaaaagagt ctttattatg
     6541 ttatttagag aataattttg cgattattac ttttgcaaag tttttcactt tttcgaccgc
     6601 ttgttaaata aaaagggcga aaaatatttc gcccctaccg attactcttg tggaattgta
     6661 cggattgcta actcttccaa cgctttcgga ttcgcataac ttggtgcttc tgtcattaaa
     6721 cacgctgcag cggtggtttt cgggaaagcg atgacatcac gaatgttatc agttcctgta
     6781 atcaacatcg ttaagcggtc taaaccgaag gctaagcctg cgtgtggcgg agtaccgaat
     6841 tttaacgcat ctaataagaa accgaatttc tctttttgct cttcttcgtt aatgcctaaa
     6901 atgctgaaca cggtttgttg catctgctgg ctgaaaatac gtaccgagcc accgcccact
     6961 tcgtagccgt taattaccat atcgtaagcg tttgccaccg cattttccgg atcttgcact
     7021 aattcttccg gcgataattc tttcggtgaa gtgaacgggt ggtgcattgc cgataagttg
     7081 ccttcatcat cacgttcaaa cattgggaaa tcaatcaccc ataacggttt ccacgctgtg
     7141 gtatcggtta agcctaaatc gttccccact tttaagcgta acgcaccgat ggcgttagtc
     7201 gcaacgtgcc agttatccgc accgaagaat acaatgtcgt ctttttgcaa gtttaagcgt
     7261 ttaactaagg cttttagcac ttctgcattt aagaattttg caaccgggct ttgaatgcct
     7321 tctaaacctg tgttcatatc attcactttt aaccaagcca agccttttgc cccataaatg
     7381 ccgacaaatt gggtgtagtc atcaatattt ttacgggtta gagctgcacc gtttggcaca
     7441 cgaagtgcca ctacacgccc tttcggatca ttcgccggac cgctaaatac tttgaaatca
     7501 accgatttta aaatatctgc cacatcaacc atttccagtg ggttacgcaa atccggtttg
     7561 tctgaaccga aacgggtcat tgcttcattc cacgtcatca ttgggaattt accgagatca
     7621 acgcctaagg tctttttcca taagccgtgg atcatcgctt ccattaatgc acgcacttct
     7681 tcggcagtca taaagctggt ttccacgtct atttgggtaa attccggctg gcggtcggca
     7741 cgcaaatctt catcacggaa acatttcacg atttggtaat aacggtcgaa gcccgacatc
     7801 attagcaact gtttaaatag ctgaggtgat tgtggcagag catagaattt gcctttatgc
     7861 acacggcttg gcacgaggta atctctcgca ccttcaggag tagctttggt cagcatcggg
     7921 gtttcaatgt ctaagaaacc gttatcgtcc ataaaacggc ggacaaaact ggtgatttta
     7981 gcacgggttt tgagcttttc tgccatttca gggcggcgta aatccaaata gcggtatttt
     8041 aaacgctgtt cttcggtatt gttttgattg aagtctaacg gcaatacttc agcgttgtta
     8101 tacaccacta aattttttac taacacttca atttcaccgg ttgccatctc tttattgatt
     8161 tgcgattcat cacgggcaat cacttcacct tgaatttgca cgcacgcctc agaacgtaag
     8221 gccgatgctt gcttgaaaag tgcttcatct ttctcatcga aaaacacctg cacaatgcct
     8281 tcacgatcac gaatttgcat aaaaataaaa cgaccaaggt tacgaacacg atgaacccaa
     8341 ccgcttaagg taactgtttg tccaaggtgt gagcggttta atgcaccgca gtagtgagaa
     8401 cgcatcataa aattatcctt ttgaaaataa atatcccaag tttcggggaa aacttaagga
     8461 ttatagcgga aaattcagcg aagatggata aataaaagca aacaagcggt gatttttatt
     8521 ccattttttg caaaaagtgg gataaaaatc accgcttgtg atttatcctt ccagcccaat
     8581 aaaaacggct aataacaatg gagcggctaa attaacaata aaaccgaagc tgattgccag
     8641 cggcacaatt tgaataccgc ccgatttttg aataataggg agcatacaat ctaatgaagt
     8701 cgctccgcca atgccgactg cggtggacgg aaattgacgc ataaacagtg gtactaaaaa
     8761 caggcataaa atttcacgcg agatgtcgtt aaaaaaggca atactgccgt aaatcgggcc
     8821 ccaagcatcg tttaccaata cgcttgaaag ggaataccaa ccgaatgcag aggcaaaggt
     8881 taagccttta gcaagggata aatctaaggt gaatgccgaa atcacgccac caatcaggga
     8941 acttgccacc attacaatcg aggtataaat cccccgtttg ttaaagaaca cttcacgcag
     9001 gggaataccg ctgtttcgca gttgaatccc caccccgaaa atcatcactt ctaacacata
     9061 ggtgctggcg tgcagaggga aatccaccac gcctttggtg aaatagccga tgatcccgcc
     9121 taataaggta atgccgatta atttaaagga atcaatcaac aaatgcaggc gggagggaag
     9181 ttgatcttga ttgactttgt ctcttttcat cggttgtaat ttgtcgtaaa tcaccaagcc
     9241 gataatgttt gaaaggagta aaattaccac taaactaaac gccgtaccgc caatctgtgg
     9301 cagttcggtg agaatgttat caatttgtcc gagagaaatc cccatcacta ataaaatcaa
     9361 gtaaaggcag aggctgacca ctctgtttac tattgccata tagcggtggt ttttgagggt
     9421 tattaagtaa ccgagaaaaa gtggaatcag cacaatggct aaaccgtaga gcatttttta
     9481 tcctttttat cgctattttt aaacttgaaa ttctaacaat aagggcataa aataaaactg
     9541 tttttatata cagttatttg tgttgcaagc ggtcgttttt ggggaaaata ttgcaaatgt
     9601 cgattcaagc taaacataag cgaaaaatta tccatattga tatggattgt ttttatgcag
     9661 cggtcgaaat gcgggaaaac ccggcgttgg tcggtaagcc ggtggcggtg ggcggcaatg
     9721 ctaatcaacg aggtgtgctg acaacctgta attacgaggc tcgaaaattc ggcttacaca
     9781 gtgcaatgcc aacggcaact gcgttgaaga aatgcccgaa tttaatttta ttgccggtga
     9841 atatggcgtt gtataaattg gtttccgctc aaattcacga aatttttcat cgctatacgg
     9901 atgtgattga agccttgtcg cttgatgaag cctatttaga tgtaaccgaa tgcgaacgct
     9961 attttggctc cgccacttgg attgctcaag caatccgcaa tgatattttc aatgaattgc
    10021 atttaactgc ctcggcagga gttgctccgc ttaaattttt agcaaaaatt gcctccgatc
    10081 aaaacaaacc taacggacaa tgtgtgatta cgcctgatga tgtgcaaaat tttgtgaaaa
    10141 atctaccgct tgcaaaaatt ccgggtgtgg ggaaagtaac caatgaaaaa ttaaaacagc
    10201 ttggctttga aacctgtggt gatattcagc aagcagaaaa agcggttatt taccgagcgt
    10261 ttggcaaatt cgggcaacgg ctttgggatt ttagccacgg tattgatgaa cgagcggtag
    10321 agtcttatcg tgaaagaaaa tcgctggcgg tggaaaatac gctgttgcac gatatttcaa
    10381 cccttgagca agcggaaaag attgtggcag atttattcga caaactggtt tttcggttgc
    10441 aacgaaactg gggcgatacg ccattaactg agtttaaaaa gttaggcatt aaactcaagt
    10501 ttgatgattt tagccaaacc acgcttgaac gaaccaccga tggctttgcg tatgagcgat
    10561 ttggcgagtt gctacagcaa atttttaccc gaagaaagga acgaaaggtg cgattaatcg
    10621 gtctaagtgt tcactttccc gagaaaaaag tgaataaaca gttaaatttg tgggaataat
    10681 tgtaaataat gacttgaaat taaggctaaa gtcattatct tattcgtgca atcattgcat
    10741 ctatattttt ggagattgaa aacattatgg caaaacgtgt cattctattt ttattaacta
    10801 acttagcgat tacctttgtt ttagggatcg tattaaacct tatttttaaa gtgaccggca
    10861 ttcaagggca aagcactgcc ggtattttgg tgctttcgtt agtcttcggt ttcagtgggt
    10921 ctttaatttc gctatttatg tcgaaatcaa tggcattgcg ttcggttggg gcagaggtga
    10981 ttacacagcc tcgtaacgaa gcggagcaat ggttattcaa cactgtacaa cgtcaggctc
    11041 agcaagcgaa tatcccaatg ccggacattg cgatttacca ttcggctgat gtaaatgcat
    11101 tcgctacagg agcaacgaaa agtaattctt tagttgcagt cagcaccggc ttgctgaatt
    11161 caatgactaa agacgaagct gaagcggtag tagcacacga agtgg
//
LOCUS       DANJWC010000077        10902 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.077, whole genome shotgun sequence.
ACCESSION   DANJWC010000077 DANJWC010000000
VERSION     DANJWC010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10902
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.077"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            37..369
                     /locus_tag="PWX02_001422"
     CDS             37..369
                     /locus_tag="PWX02_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDL1729430.1"
                     /translation="MALSVSSILSSQATYAEETTAELSEVNVVAKEDVKASIEKKNKE
                     IIQQELIQNNRDLVRYSPDVGIVNQGRHQKGFAIRGVEDNRVGISIDGVSLFLIQKKI
                     PFINAMVI"
     gene            336..2414
                     /locus_tag="PWX02_001423"
                     /pseudo
     CDS             336..2414
                     /locus_tag="PWX02_001423"
                     /inference="COORDINATES: protein motif:HMM:TIGR01786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
     gene            2469..2975
                     /gene="hutX"
                     /locus_tag="PWX02_001424"
     CDS             2469..2975
                     /gene="hutX"
                     /locus_tag="PWX02_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HDL1729431.1"
                     /translation="MISIEQQTALRNELAENPGQILEMLAAKYQCTLEEVMLNLPAEM
                     LKVTEGERFAEVLQQIHEWDDAITLISHTEDTIIEFVGKLPSGSISRGFYNFEHKEGG
                     GLQGHLRYENCAKIYLLDRPFMGKRTVSLIFINKKGNAMFKIFVGREKIGGALKEHQI
                     KALYQLIG"
     gene            complement(3055..4272)
                     /locus_tag="PWX02_001425"
     CDS             complement(3055..4272)
                     /locus_tag="PWX02_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter"
                     /protein_id="HDL1729432.1"
                     /translation="MKNKILGSALMIAGTTIGAGMLAMPLTSAGMGFGATALLLVGLW
                     ALLAYTGLLFMEVYQTAPQKDVGVASLAEQYFGLIGRVLATFSLLVLLYALLAAYITG
                     GGSLLAGVMPEMADADMKLKISILIFTAVLGTFVVVGVKSVDGLTRVLFLGKIVAFIA
                     VLLMMLPKAKLENLTAIPLDNLLIISAIPIFFTAFGFHVIMGTINSYLDADIGKIRKS
                     IYIGTAIPLVAYLLWQLATHGVLSQNEFTTILTQDPTLNGLVKATSQITGSTILGEMV
                     RLFSVLALITSFLGVSMGIFEGVGDLLKRLNLPTNRLWLTIATFTPPILFALFYPNGF
                     IKALGYAGLLFAFYGMILPIGLAWKARRQYPNLPYRVIGGNLALVVALIAGIIIMVIP
                     FLIQAGYLPQTVG"
     gene            4433..7966
                     /gene="mfd"
                     /locus_tag="PWX02_001426"
     CDS             4433..7966
                     /gene="mfd"
                     /locus_tag="PWX02_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDL1729433.1"
                     /translation="MSLISLNLPKSDGNFQDHQTLGNLVGYSDTLAISQAARAFYGLT
                     VVVTPDTRTALRLEKALPQFAGLPVQLFPDWETLPYDNFSPHQDIISARLSALFELQQ
                     GRQQILLLPINTLMQKVCPPNYLANNVLLIKKGDRFSIQKLRLQLENAGYRAVEQVLE
                     YGEYAVRGSILDLFPMGAEEPFRLDFFDDEIDSIRTFDVDNQRTKTEIAEINLLPAHE
                     FPTDSNGIEHFRVKFREKFGEIRREPEHIYQQVSKGILNAGIEYWQPLFFEEMASLFD
                     YFPAQTLFITFADIQQKAEQFHQDTQNRYESRRVDPMRPLLPPSDLWFAIDEVNRWLK
                     GYPRLTITSEKIRKSAAKMNANVANLPDIAIQSSAKEPFSAFQQFRQKFDGNILFSVE
                     SEGRRETLLDLLAPLGVKPKQVNSLDEAQGLPFSLIISPLDQGFVVETGGHGTRPYDD
                     ELNTNGENDNNYVGAGPVPARNLAIICETDLLGERVQTRREKNRKAVNPDTLIRNLAE
                     LKIGQAVVHLENGVGRYGGLTVLDAGGIKAEYLVLHYANDAKLYVPVASLHLISRYIG
                     GADETAPLHKLGSEAWAKTRQKAAEKIRDVAAELLDVYAKRESQTGFAFAYDKAEFDQ
                     FSATFPYEETDDQKIAINAVISDMCQAKAMDRLVCGDVGFGKTEVAIRATFLAVMNHK
                     QVVILAPTTLLAQQHYENFKDRFANYPVNVEVLSRFKTAKEQKTILQNVAEGKVDILV
                     GTHKLLQDDVAFRDLGLLVIDEEHRFGVRQKEKIKQLRANVDILTLTATPIPRTLNMA
                     LNGMRDLSIIASPPARRLLIKTFVRQSDEMVIREAILREILRGGQVYYLHNDVATIEN
                     CAEKLAELVPEARIVIGHGQMRERELERVMSDFYHQRFNLLVCSTIIETGIDVPTANT
                     IIIERADKFGLAQLHQLRGRVGRSHHQAYAYMLTPHPKTLTKDAEQRLEAMSTIDNLG
                     AGFALATHDLEIRGAGELLGSEQSGQIESIGFSLYMDLLENAVKALQEGREPSLDEIT
                     QHQAEIELRVPALLPDDYLPDVNMRLSFYKRIASSENLQSLNDLKVELIDRFGLLPEA
                     TKNLFAITQLRLSAQTLGIKKIDAGIHGGYLEFKPTAQPDPMKFIALIQKQPAVYSFE
                     GAVKFKFRLALEDNRKRLEFVENLLREVVGG"
     gene            8129..8416
                     /locus_tag="PWX02_001427"
     CDS             8129..8416
                     /locus_tag="PWX02_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HDL1729434.1"
                     /translation="MEEINGFEWDSEKALRNQQKHGVSFIEAVSVFYDNNAMLIPDPD
                     HSSREERFLLLGRSNKDNLLVVVHCERNTNIRIISARLAAKRESGQYKGVH"
     gene            8417..8629
                     /locus_tag="PWX02_001428"
     CDS             8417..8629
                     /locus_tag="PWX02_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HDL1729435.1"
                     /translation="MRDEYDFSNAVKNPYAKGLKSKSVVTIRIEDDVISYFKQLAEEV
                     NLPYQTLINLYLKDCVEQKRKPTISW"
     gene            8745..9005
                     /locus_tag="PWX02_001429"
     CDS             8745..9005
                     /locus_tag="PWX02_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDL1729436.1"
                     /translation="MEKIARLFRNGRNQAVRLPVEFEFDAEQVYVSKQENGDILLSLR
                     SRNTEHWQRLFELLPAVVCDETFLDTKERNQSFEQRDPFEVA"
     gene            9006..9395
                     /locus_tag="PWX02_001430"
     CDS             9006..9395
                     /locus_tag="PWX02_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HDL1729437.1"
                     /translation="MYMLDTNTVSYFFRGNANVVAKLKSINPERLCISSVTAAELVYG
                     VAKRNNGQLSQFLGYFLSSVKVLDWNYRCAELYGKLRAEMEKNGKVMGVQDQMIASHA
                     LAEECILVSSDQAFKMVPDLKLENWLH"
     gene            9415..10653
                     /gene="pepT"
                     /locus_tag="PWX02_001431"
     CDS             9415..10653
                     /gene="pepT"
                     /locus_tag="PWX02_001431"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDL1729438.1"
                     /translation="MKQSLLNRLLRYTQIHTTSDPKSQTVPSSQQQWDLLHLLKTEVE
                     AMGLSEIYLDPNGYLFASLPANIDKTLPVLGLIAHVDTSPDFNGENVKPQVIEAYQGG
                     NILLNGSGDYLSPTDFPNLNKLIGDTLVTTDGTSLLGADDKSGIAIILSAIEYLQANP
                     HIPHGKIRIGFTTDEEIGRGADHFDVNAFGADVAFTLDGGPLGELQYENFNADAATVT
                     FFGRSVHPGTAKGKMINALERACEFQHRLPPHLTPATSEGREGFIMLHGIEGGIDKAE
                     IHYILRSFERDELAAFGKMLNDTLAEMQQIYGEQCGKVEIVEQYRNMREVLDDYGYLV
                     EIAENAMREQGIEPNLEPIRGGTDGSRLSFMGLPCPNLFTGGENFHGRFEFISVDTME
                     KATKVVIDICRKFADYDFNK"
     gene            10820..10895
                     /locus_tag="PWX02_001432"
     tRNA            10820..10895
                     /locus_tag="PWX02_001432"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10853..10855,aa:Gly,seq:gcc)
BASE COUNT         3257 a         2180 c         2505 g         2960 t
ORIGIN      
        1 tattcaataa agtctaaaaa attgtgcccg actatattgg ctttaagcgt tagtagcatt
       61 ttgtcttcac aagctaccta tgcggaagaa acaacagcgg aattatctga agtaaatgtt
      121 gttgctaaag aagatgtgaa ggcttctata gaaaagaaaa ataaagagat aattcagcaa
      181 gagttgattc aaaataatcg agatttagtt cgttatagcc ctgatgttgg cattgttaac
      241 caaggtagac atcaaaaagg atttgctatt cgtggagtgg aagacaaccg tgtaggaata
      301 agtattgatg gtgtctcgct tttcctgatt cagaagaaaa ttccctttat aaacgctatg
      361 gtaatttaaa tacatctcgc caatctatag atccggaatt agctcgtact attgaagtat
      421 ctaaaggtgc agattctttc aatcaaggta gtggtaattt aggcgacgga gttaattatc
      481 ggacgcttga gccttatgat attgttcgta acggtaataa atttggtgct ttttatcgca
      541 caggatatgg tactcgtaat aatgaatggt tgaatacatt cggtgttgga tatttaggtg
      601 agaaagctga ggcgatattg gtttattcaa atcgccatgg gcatgaaatg aaaagtgcag
      661 gaggttatac acttcctgaa gattctctac aaactcgctc tttacgcagt agtaaacaaa
      721 ctcctgatga ttctactcat aatcatcata attatttagc caaatttgct tatcgattta
      781 atgataatca ccgcgttggt gtttcttaca gtgggcaaaa taataaaaat tatattatcg
      841 aagattcagc cgttactatt acttcttttt ggcgtgaagc ggaagaccgt agtaaacgtg
      901 atacagtaaa tgttttttat gagtatttcc cagagtcaaa atggatatct ctagcgaaga
      961 tagatgcaga ttatcaagat actgaaacat ctgcttataa ctatgaaggt acacgcgctg
     1021 aagatgcaac agactggagc cctgttaaac aaagaaaacc aagtgacaat aatattcgta
     1081 tttttaatac tgaattgaaa cgcttaaatt tccgtttaga cagccaagca ttagaatttg
     1141 gtaaatggac tcatcagcta tctttcaaag cctctactgc agagagagat ttcgatgtat
     1201 tacataagga ctcggtttat ttatccggtg tttgggtaaa ttctcccgat tctacgatga
     1261 tgtacccaat taaaacgaaa cagcacactt tttcattgca cgataagatt gatttaacgc
     1321 atgattggat tgcaacgcta ggcattcgtt atgattgggc gaagtatgaa caacaagaac
     1381 tcaatggtct tgtttgtcgt aactgcgtaa aaactgacaa tgcgaaattt aatcaactaa
     1441 cttgggttac cggtttagaa aaacatctga ctgaggcatg gaaagtagga tataacattg
     1501 ggacaggctt ccgtattcca aatgcatcag aaatgtattt tgattaccgt gataatgctg
     1561 caggagcttg gatgtccaat ccaaacctaa aagcggaacg tagtttaagc caaaatttaa
     1621 gtttgcaagg tagtggcaat gttgggctgc tctcagttaa tttccaccat actaaatata
     1681 aagatttttt atgggagcaa gaaacctggg atgtttacca agcatatggt agagagtttt
     1741 ggcgcccggt acagcaaatg caaaatattg attctgcgaa aatctacggg ctagaagtga
     1801 gtggtaaatg gaatttaaat tcagctatgc caatacctga aggctggaag ctatttggag
     1861 ccttcggtta cagcaaaggt agtatgtcta acggggcaga tttactttct attcagccga
     1921 ttaaagccgt agttggctta gattatgaac agcctgaagg taaatgggga gtattttctc
     1981 gcttcacatt cttgggggct aaaaaggcga aagatgcaaa atatttaaaa acattaccgg
     2041 aaaaatgcct taaagaggat agaacgccaa atccgtatta cccttattgg ggggatgaat
     2101 atgacattcg ttgtactgaa tattctcacg agacaggttt agatacctgg aagcacttaa
     2161 atgctaaagc ctttgttgtg gatttatacg gtttctataa accaactgag aatattacat
     2221 tacgtgccgg tatttataat ttgtttaacc gtaaatatca tacttgggat actttacgtg
     2281 gtttaaatac aacaggtgga atcgttaatt ctgttggagt acgtgaaaat gcaacttacg
     2341 gtggctatcc tggattacaa cgctattatg aacctggacg taacttctcg gctaattttg
     2401 aataccgttt ctaatttaca aatttactta gagagctaag aaactctctt tacttaaagg
     2461 aacatttaat gatatcaatc gaacaacaaa ccgccttacg caatgaattg gccgaaaatc
     2521 cggggcaaat tttagaaatg ttagcggcaa aatatcaatg cacgctggaa gaggtaatgc
     2581 tgaatttacc ggctgaaatg ctaaaagtaa ccgaagggga acgttttgcc gaagtgctac
     2641 agcaaatcca cgaatgggac gatgcgatta cgctgatttc gcatacggaa gataccatta
     2701 ttgagtttgt gggaaagctc ccaagcggtt caatttctcg tggattttac aatttcgaac
     2761 ataaagaggg cggaggctta caagggcatc tacgctacga aaattgtgcc aaaatttatt
     2821 tgttggatcg tccttttatg ggaaaacgta ctgtttcact gattttcatt aataaaaaag
     2881 gcaacgcaat gtttaagatt tttgtcggca gagagaaaat tggcggggca ttaaaagagc
     2941 atcaaattaa ggcgctgtat cagttgattg gctaatctgt aaaaagtcat cgaaaaatga
     3001 ccgcttgtaa cttatagaaa gggctacgat attgaatcgt agcccttttc aatattagcc
     3061 taccgtttgc ggtaaatagc cagcctgaat taaaaacgga atgaccataa tgataatacc
     3121 ggcaattaac gcaaccacta atgccaaatt accaccaatc acacggtaag gtaaattcgg
     3181 atattggcga cgtgctttcc acgctaagcc gataggtaaa atcataccat agaacgcaaa
     3241 gagtaaaccg gcgtaaccta atgccttaat aaagccgttc ggatagaata aagcgaacag
     3301 aattggcggt gtaaaggtgg cgatggtcag ccataaacgg ttagtcggta gatttaagcg
     3361 ttttaacaaa tcgcccacgc cttcaaaaat tcccatcgaa acccctaaaa atgaggtaat
     3421 caatgcaagc actgagaata agcgaaccat ttcgcctaaa atcgtactac cggtaatctg
     3481 gctagtggct ttcaccaagc cgtttaaggt cggatcttgt gtcaaaattg tggtgaattc
     3541 attttggctt aataccccgt gtgtagcgag ctgccacaat aaataagcca ctagcggaat
     3601 tgccgtcccg atataaatag atttacgaat tttgccgata tctgcgtcta aatagctatt
     3661 aattgttccc ataataacgt ggaaaccgaa cgcagtgaag aaaatgggta tagcagaaat
     3721 aatgagtaag ttatctaatg gaattgctgt caagttttcc agcttcgctt taggcagcat
     3781 catcagcaac acggcaataa aagcaacaat ttttcccaaa aacagtactc gggttaaacc
     3841 atcaaccgat ttcacgccga ctaccacaaa tgtacctaat accgcagtga agattaaaat
     3901 gctgattttc agtttcatat cagcatctgc catttcaggc attacaccgg ctaacagtga
     3961 accaccaccg gtaatataag ccgcaagtag ggcataaagt agtacgagca aactgaaagt
     4021 ggctaataca cgcccgatta agccaaagta ttgctcggca aggcttgcca ctccaacatc
     4081 tttttgcgga gcggtttgat aaacttccat aaataataaa ccggtgtagg ccaataaagc
     4141 ccataatccg actaagagta atgcggttgc cccaaaaccc attccggcag aggtgagtgg
     4201 cattgcaagc attcctgcac caatggtagt gcctgcaatc attaatgcac ttcctaaaat
     4261 tttatttttc ataatatgtg tgagtaagag ttaaaaaagg ggaacatttt atcagaattt
     4321 tttcgcaatg cgatctagat aacataattt ctccgcttat tttgcacaat cagccgtgct
     4381 ttagtaaact aagcaacaat ttgcaatttt ttatttaaaa cagaccgctt gtatgtcttt
     4441 aatttcactt aatctgccga aatctgacgg caattttcaa gatcatcaaa ccctcggtaa
     4501 ccttgtcggg tattccgata cccttgcgat cagccaagct gcccgagcct tttacggctt
     4561 gacggtggtt gtcacacccg acacccgtac ggcgttacgc ttggaaaaag ccttgcctca
     4621 gtttgcaggg ctgccagtgc aactgttccc cgattgggaa accttgcctt atgacaattt
     4681 ttcgccgcac caagacatta tctccgcccg actttctgcc ctgtttgaac tgcaacaagg
     4741 gcgacagcag attttgctgt tgccgatcaa caccttaatg cagaaggtct gcccgccgaa
     4801 ttatctcgct aacaatgtgc tgttgattaa aaaaggcgac cgtttttcga ttcaaaaact
     4861 gcggttacaa ctggaaaatg ccggctatcg ggcggtggaa caggtgttgg aatatggtga
     4921 atacgccgtg cgtggctcga ttttagactt attcccgatg ggagcagaag agccgtttcg
     4981 cctcgatttt ttcgatgatg agatcgacag tatccgcact tttgatgtgg ataaccaacg
     5041 caccaaaacg gaaattgccg aaattaattt actgccggca cacgagttcc cgactgatag
     5101 caacgggatc gagcatttta gagtcaaatt ccgtgaaaaa ttcggtgaaa tccgccgtga
     5161 gccagagcat atttatcagc aagtcagcaa aggcatttta aatgcaggga ttgagtattg
     5221 gcagccgttg ttttttgagg aaatggcaag tctgtttgac tatttccctg cccaaacgct
     5281 gtttatcact ttcgccgata ttcagcaaaa agctgaacaa tttcaccaag atacccaaaa
     5341 ccgctatgaa agccgccgag tggatccgat gcgaccgcta ctgccgccga gcgatctctg
     5401 gtttgcgatt gatgaggtaa atcgctggct gaaaggctat ccccgtttaa cgataacttc
     5461 ggagaaaatc cgtaaatcag cagcgaaaat gaatgcaaat gtggctaact tgccggatat
     5521 tgctattcaa tcctctgcca aagagccgtt ttctgctttc cagcaattcc gccaaaaatt
     5581 tgacggcaac attctgtttt cggtggagag cgaagggcga agagaaacct tgctggattt
     5641 gcttgctccg cttggggtga agccgaagca ggtcaattca ttagatgaag cacaaggttt
     5701 gccattttcc ctgattattt caccgcttga tcaagggttt gtggtggaaa cgggcgggca
     5761 cgggacccgc ccctacgatg atgaattaaa tacaaatggc gaaaatgata ataactacgt
     5821 aggggcgggt cctgtgcccg cccgcaacct tgccatcatc tgcgaaaccg acctgctggg
     5881 cgaacgtgtg caaacacggc gtgagaaaaa ccgcaaagcc gtcaatcctg ataccttaat
     5941 ccgtaattta gcagaactca aaatcggaca ggcggtggtg catttggaaa acggcgtggg
     6001 gcgttacggt ggcttaaccg tgctggacgc tggtgggatc aaagccgaat atctcgtgct
     6061 acattatgct aacgatgcca aactctatgt gccggttgcc tcgctccatt taatcagccg
     6121 ttatatcggt ggggcggatg aaactgcgcc gctacataaa ttaggcagtg aagcgtgggc
     6181 gaaaacccgc caaaaagcag cggaaaaaat ccgtgatgtg gcggcagaat tgcttgatgt
     6241 ttatgccaaa cgagaaagcc aaaccggttt tgcgtttgcc tacgataaag cggaatttga
     6301 tcagtttagt gccactttcc cttatgagga aacggacgat cagaaaattg ccatcaatgc
     6361 cgtaattagc gatatgtgcc aagccaaagc gatggataga ttggtgtgtg gcgatgttgg
     6421 cttcggcaaa accgaagtgg ccattcgagc gactttcttg gcagtgatga accataaaca
     6481 ggtggttatt cttgccccaa ctaccttgct cgcccaacag cattatgaga atttcaaaga
     6541 ccgtttcgcc aattaccctg tgaatgtgga agtgctgtct cgctttaaaa ccgccaaaga
     6601 gcagaaaacg attttgcaaa atgtggcgga gggcaaagtg gatattttgg tcggcacgca
     6661 caaactgttg caagacgatg tagcgttccg tgatcttggg cttttagtga ttgatgaaga
     6721 acaccgtttc ggcgtacgcc aaaaagagaa aatcaaacaa ctgcgtgcca atgtggatat
     6781 tctgaccctc accgccacgc caattccaag aacgctgaat atggcactca acggaatgcg
     6841 tgatctctcg attatcgcca gcccgccggc tcgccgactt ttaatcaaaa cctttgtgcg
     6901 acaaagtgat gaaatggtaa tccgtgaggc aattttgcgt gaaattctgc gtggtgggca
     6961 ggtttactac ctccataacg atgtggcaac cattgaaaat tgtgccgaga aattggctga
     7021 actcgtgcca gaggcaagaa ttgtgatcgg acacgggcag atgcgtgagc gtgaattgga
     7081 gcgggtgatg agcgatttct atcatcaacg ctttaacctg ctggtttgct ccaccattat
     7141 tgaaacgggg attgacgtgc cgactgccaa taccattatt atcgaacggg cggacaaatt
     7201 cgggctggca caactgcacc aactgcgtgg acgggtcgga cgctcgcacc accaagccta
     7261 cgcctatatg ctcacaccgc acccgaaaac gctcaccaaa gatgccgagc aacgcttgga
     7321 ggcgatgagt accattgata atctcggggc cggttttgcg ttggcgaccc acgatttaga
     7381 aattcgtggt gcaggcgaat tgctcggttc ggagcagagc ggacagattg aaagcatcgg
     7441 tttttcgctc tatatggact tgctcgaaaa tgcggtcaaa gctttgcaag aagggcgaga
     7501 accgagcctc gatgaaatca cccaacacca agcggagatt gaattgcgtg taccggcctt
     7561 attgccggac gattatctgc ctgacgtgaa tatgcgtctg tcgttctaca agcggatcgc
     7621 aagctcggaa aatctgcaaa gcctgaacga cttgaaagtg gagctgatcg atcgcttcgg
     7681 cttgctccca gaggcaacca aaaacctgtt tgcgattacc caacttcgct tgtcggcaca
     7741 aacgctcggg attaagaaaa tcgatgccgg cattcacggc ggttatcttg aatttaaacc
     7801 gacagcccag cccgacccaa tgaagtttat cgccttaatc caaaaacaac cagcggtgta
     7861 cagttttgaa ggggcggtga agttcaaatt ccgcttagca ctcgaagaca accgcaagcg
     7921 gttagaattt gtggaaaatt tgctaaggga ggttgtgggc ggataaagcc tcctaaggtt
     7981 gtcaatcttg aaaaacgatt tttgagatct gtatcgtaaa aatcgttttt gtttattgcg
     8041 aatccagtaa tttcatctag gctgtttata acttaaaaaa atagatgtaa atatatttta
     8101 tttacaatca aaaaggagta aactggcaat ggaagaaatt aatggttttg aatgggatag
     8161 tgagaaagct cttcgtaatc aacaaaaaca cggagttagt tttatcgagg cggtaagcgt
     8221 attttatgat aataatgcga tgttaatccc ggatccagat cactcatcta gggaagagcg
     8281 ttttttgcta ctcgggcgta gcaataaaga taatctactt gtggttgtgc attgcgagag
     8341 aaataccaac attcggatta tttcagcacg tttggcagca aaacgcgaaa gtgggcaata
     8401 taaaggagta cattaaatga gagatgaata cgatttttct aatgcagtga aaaaccctta
     8461 cgcaaaaggg ctaaaaagca aaagtgtggt tactattcgg attgaagacg atgttatcag
     8521 ctattttaaa caacttgccg aagaggtgaa tttgccttac caaacgttga tcaatcttta
     8581 tttgaaagat tgcgttgagc agaaacgtaa accgactatt agttggtaat attatgattt
     8641 gaaatcagta tgttattgag tatttgagaa tgggttatct tgccagttgt ataaaatagg
     8701 catatacttt ggcatataca ctatctatcg gagggtaaag taggatggaa aaaattgcga
     8761 gattattccg taacgggcgg aaccaagcag tgcgtttacc ggttgaattt gagtttgatg
     8821 cagagcaagt ctatgtttct aaacaagaaa atggcgatat tttattatca ttgagatcga
     8881 gaaataccga gcattggcaa cgattgtttg agctattgcc tgctgttgta tgtgatgaaa
     8941 catttttaga tacaaaagag agaaaccaat cctttgagca acgggatcct tttgaggtag
     9001 cttaaatgta tatgttagat acgaatactg taagctattt ttttcgtgga aatgcaaatg
     9061 tggtggcgaa attgaagtcc attaatcctg agaggctttg tatttcatct gtaactgctg
     9121 ctgaattggt ttatggtgtg gcgaaaagaa ataatgggca gctttctcaa tttttaggtt
     9181 attttttatc gagtgtaaag gttttagatt ggaattatcg ttgtgctgaa ctttatggaa
     9241 agttacgggc agagatggag aaaaatggca aagtaatggg cgtgcaggat caaatgattg
     9301 ctagtcacgc tcttgctgaa gagtgtattt tagtttctag cgatcaggca tttaaaatgg
     9361 tacctgatct gaaattggag aattggttac actaaactat agtaaggaaa aacaatgaaa
     9421 caatcccttc ttaaccgttt actacgttac acccaaattc acacaacttc agatccgaaa
     9481 agccaaactg tgccaagctc gcaacagcag tgggatttgc ttcatctgct gaaaacggaa
     9541 gtagaggcga tggggctaag cgagatttat cttgacccca acggctatct gttcgccagc
     9601 ttgcctgcca atattgacaa aaccctgccg gtgcttgggc tgattgccca cgttgatact
     9661 tcgcccgatt ttaatggtga aaacgtgaaa ccacaggtga ttgaggctta tcaaggcggc
     9721 aatattttgc taaacggcag tggcgattat ctttcgccaa cggatttccc gaatttaaac
     9781 aagctgattg gcgacaccct agtcaccact gacggcacct ctttgcttgg cgcagacgac
     9841 aaatccggca ttgcgattat tctctcggct atcgagtatt tacaagccaa tccgcacatt
     9901 ccgcacggca agatccgcat cggctttacc accgatgaag agatcggtcg aggcgcagat
     9961 cattttgatg taaacgcatt cggtgccgat gttgccttta cccttgatgg cgggcctctg
    10021 ggcgaattgc aatatgaaaa tttcaatgcc gatgcagcga ccgttacgtt cttcgggcgg
    10081 agcgttcacc ccggcacggc aaagggcaag atgatcaacg ccttagagcg agcttgcgag
    10141 ttccaacacc gtctgcctcc ccatttaacc cctgccacca gcgaagggcg tgaaggcttt
    10201 attatgttgc acggcattga gggtggaatt gataaagcgg aaattcacta tattttacgt
    10261 agctttgagc gtgatgaatt agctgcattc ggcaaaatgc tgaatgatac gctggcagag
    10321 atgcaacaga tttacggcga acagtgcgga aaagtcgaga ttgtcgagca atatcgcaat
    10381 atgcgtgaag tgctggacga ttacggctac ttggttgaaa ttgccgaaaa cgcaatgcgt
    10441 gaacaaggca ttgagccgaa tttagaaccg atccgtggcg gtactgatgg ctcacgcctt
    10501 tcatttatgg gcttgccttg cccgaattta tttaccggcg gcgaaaattt ccacggacga
    10561 tttgagttta tttcggttga tacgatggaa aaagccacaa aggttgtaat tgatatttgt
    10621 cggaaatttg ctgattatga tttcaacaaa taaggcggct gcaagcggtc aaatttgatg
    10681 aaaaatttgc aaaaagcgtt atttttcacc aattaaacga ttttttacag atttcctaca
    10741 aatttatttg acattaaatc tgaaatccat agaatatgca cctgttgaga cctctacgag
    10801 gttcgtagcg tttttaaatg cgggaatagc tcagttggta gagcacgacc ttgccaaggt
    10861 cggggtcgcg agttcgagcc tcgtttcccg ctccaatttt tc
//
LOCUS       DANJWC010000078        10339 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.078, whole genome shotgun sequence.
ACCESSION   DANJWC010000078 DANJWC010000000
VERSION     DANJWC010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10339
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.078"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..2549)
                     /gene="mutS"
                     /locus_tag="PWX02_001433"
     CDS             complement(<1..2549)
                     /gene="mutS"
                     /locus_tag="PWX02_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL1729439.1"
                     /translation="MTQDFSTHTPMMQQYLQLKAQNPDILLFYRMGDFYELFYDDAKR
                     AAALLDISLTKRGQSAGEPIPMAGVPYHAVEGYLAKLVALGESVAICEQIGDPATSKG
                     PVERKVVRIVTPGTVSDEALLPERQDNLVAAVYEEKGTFAIATLDMTSGRFLISELAS
                     KEALSAELQRVQPAEILYAEDFSAASILTNYKGLRRRPVWEFELVTAIQLLNRQFGTQ
                     TLAGFGVEKAVVALCAAGCVLHYAQETQRTALPHINAIHLAQHSDTILLDAATRRNLE
                     LTQNLAGGTENTLAAVLDKCVTPMGSRLLKRWIHQPIRDCGKLQARQKAITALIQYER
                     MSDLQPLLQQVGDMERILARVALRTARPRDLTRLRTALAQLPEIAKNSQNLTASIDQL
                     LAQLADFSPLHDLLERAIIENPPQLIRDGGVIAEGYNAELDEWRQLSDGATQYLENLE
                     IRERESTGIDSLKIGFNAVHGYYIQISQGQAHKAPIHYVRRQTLKNAERYIIPELKTY
                     EDKVLKAKGASLALEKQLYDELFDVLMPRLGELQLAAMMLSELDVLTNLAERADSLGY
                     VCPSFSPMRMVNIKGGRHPVVEQVLTEPFIANPVFLNAQRHLLIVTGPNMGGKSTYMR
                     QIALITLMAYIGSFVPAESAEIGPIDRIFTRIGASDDLASGRSTFMVEMTEMANILHQ
                     ATENSLVLIDEIGRGTSTYDGLSLAWACAEWLAKKTQSLTLFATHYFELTGLPNQLKG
                     VANVHLDAREHQDSIVFMHSVQEGAASKSYGLAVAALAGVPKQVIQLAKQRLAHLEEI
                     SHQTKQANEHPQADLLFSDGFDLQKNEAIQPLVVENEVEKALKALQPDELTP"
     gene            2699..3262
                     /locus_tag="PWX02_001434"
     CDS             2699..3262
                     /locus_tag="PWX02_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5358 domain-containing protein"
                     /protein_id="HDL1729440.1"
                     /translation="MLRKIVLLSTGLLLAACSSNEPQQPEAFPGEFANADYVLSEENA
                     QKWVIASEQAKQCIYPNLTRIQQEHFSKEDSYIFSQYVFFYPLEEIIGEQYVKIIQND
                     EKSMGYAQYLFKKLKNNEAVEALPKKQCATLRTNAKNDLAVVKGQYQSGMIEETNSQE
                     GKSGDKVATNDNKFFFDIIKWGSMLLL"
     gene            3436..4065
                     /locus_tag="PWX02_001435"
     CDS             3436..4065
                     /locus_tag="PWX02_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDL1729441.1"
                     /translation="MAVSATSFAQTTQPTAAEQMNAVKASVAEKATVAKEATKEKAAE
                     LKAGSAEKMATAKAKTESFKDSAKNKMDAVKATTAEKTDVIKAKAASASDTMKVKAQE
                     AKAATAEKAATAHETLNSAKTATAEKAAPMKAAATEKMSAVKKSQKVNINTANEEQLQ
                     SLTGIGEAKAKAIVEYRKKHGKIKNAAELANIPGIGDATIEKVAPYLTF"
     gene            4213..4842
                     /locus_tag="PWX02_001436"
     CDS             4213..4842
                     /locus_tag="PWX02_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL1729442.1"
                     /translation="MALDFNSVGLDSVEAKGGYGIGLQIGQQLLGSGMDVNAEAVARG
                     IYDVLNNNEPAIDINEVTAALQELGQRAEAAQAEAFKAIDAENKAFLEENKKANGVIV
                     TDSGLQYEILTEGTGEKPTATSTVRVHYTGSLIDGTVFDSSVKRGQPAEFPVNGVIRG
                     WTEALQLMPVGSKWRLTIPQELAYGERGAGASIPPFATLVFEVELLDIL"
     gene            4908..5423
                     /locus_tag="PWX02_001437"
     CDS             4908..5423
                     /locus_tag="PWX02_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HDL1729443.1"
                     /translation="MQKKSLISRLLKNIFLYGSLFIIISLVVDWYRSPSAPAQFEQTV
                     HYDIQHQPKVIAQLSHEKPMLLYFWGSWCHYCQVVSPNIQQLANDGTEVLGVALKSGD
                     ENDVQAYLNENGYTFATLNDPSGDFSRGWNIQATPTILIIKDGKIAHHTTGYTSYLSL
                     KFRLWLADVLY"
     gene            complement(5471..6525)
                     /locus_tag="PWX02_001438"
                     /pseudo
     CDS             complement(5471..6525)
                     /locus_tag="PWX02_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009500034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            6722..8080
                     /gene="mnmE"
                     /locus_tag="PWX02_001439"
     CDS             6722..8080
                     /gene="mnmE"
                     /locus_tag="PWX02_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDL1729444.1"
                     /translation="MKDTIVAQATPIGRGGVGILRVSGPLAETVAQAVLGKTLPPRIA
                     NYLPFKDEDGTVLDQGIALFFKAPNSFTGEDVLELQGHGGQVILDLLLKRILQVKGLR
                     IARAGEFSEQAFLNDKLDLAQAEAIADLIDATSEQAARSALKSLQGEFSNKINALVDS
                     VIYLRTYVEAAIDFPDEEIDFLADGKIEAKLNEIIAQLDGVRREAKQGSILREGMKVV
                     IAGRPNAGKSSLLNALAGREAAIVTNIAGTTRDVLREHIHIDGMPLHIIDTAGLRDAS
                     DEVERIGIQRAWEEIAQADHVLLMIDSTEQQADQFRQEWAEFLAKLPANMPVTVIRNK
                     VDLSGEQEGLIQVDDFTMIRLSAQTKVGVDLLREHLKKSMGYQSSTEGGFLARRRHLQ
                     ALETAAEHLERGHIQLTQFLAGELLAEELRMVQNALSEITGQFTSDDLLGNIFSSFCI
                     GK"
     gene            8081..9346
                     /gene="tilS"
                     /locus_tag="PWX02_001440"
     CDS             8081..9346
                     /gene="tilS"
                     /locus_tag="PWX02_001440"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDL1729445.1"
                     /translation="MTLFNRFQTQCATHLPNQTAFLIGLSGGVDSVVLLHLFARTSFH
                     VRAIYVHHGLSPNADDWAAFCEQYCKRLNIPFILQKVRVDSSNGVENGAREARYQAIQ
                     QQLKPNEVFATAHHLDDQAETFLLALKRGSGVKGLSAMQAVTFLQNFTVFRPLLTFTK
                     SDLMGYAVQHQLGWIEDESNADNRYDRNFLRNSILPLLNQRWQHFSQMVARSAQHCAE
                     QQALIEELLSDELKSRTGEKQQLNINGFGQFSLAKQQQLSRLWLEQNGVRMPSQAQLQ
                     AVISELIFAKADKNPQVKVGENIIRRYQQAIYVTEEIADIPPFEVVLPTETKVELLYQ
                     LGTIIRQQQEIICKKNGKNHRLQLPQELAQSTISIKIGQLGKVKCYGKAQREEMKKIW
                     QNHGIPVWERNRTPLIFWQDELVGLLPIL"
     gene            complement(9336..10229)
                     /gene="xerD"
                     /locus_tag="PWX02_001441"
     CDS             complement(9336..10229)
                     /gene="xerD"
                     /locus_tag="PWX02_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDL1729446.1"
                     /translation="MAELDPIIEQFLDTLWQEHGLSENTVASYRSDLERFSDYFSEPK
                     AFLSLDHIDLQGFLGERLEQGYKATSSARMLSCLRKFFRFLCLEHYRQDDPTLTLSSP
                     KRGASLPKSLSEDQVMDLLDAPNTLDPIELRDKAMLELLYATGLRVTELVSLSMDNIS
                     LKQGGVRIIGKGDKERLVPLGEEANYWIQEFFQYGRPILLNNQQSDVVFPSRRGQQMT
                     RQTFWHRIKHYAVLAGIDADKLSPHVLRHAFATHLVNHGADLRVVQMLLGHSDLSTTQ
                     IYTQVAKARLKSLHQQFHPRG"
BASE COUNT         3080 a         2406 c         2242 g         2611 t
ORIGIN      
        1 ggggtaagtt catcaggctg aagggctttc agggcttttt caacttcatt ttctactaca
       61 agcggttgaa ttgcttcatt tttttgcaaa tcaaaaccat cggaaaacaa caaatccgcc
      121 tgtggatgtt cgtttgcttg tttggtttgg tgggaaattt cctccaaatg tgccaaacgc
      181 tgctttgcca gttgaatcac ttgctttggc acacccgcca aggctgccac cgccaagccg
      241 taacttttgc tggcggcccc ctcttgcacc gagtgcataa acacaatact gtcttgatgt
      301 tcacgcgcgt ctaaatgcac atttgccacc cctttgagct gattcggcaa gccggtaagt
      361 tcaaaatagt gggttgcaaa caaagtgagt gactgggttt tcttcgccag ccattcggca
      421 cacgcccacg ctagcgataa accgtcatag gttgaagtgc ctcgcccgat ttcatcaatc
      481 aacaccaagc tgttttccgt agcttggtgc agaatatttg ccatttcggt catttccacc
      541 ataaaggttg agcgaccaga ggctaaatca tcgcttgccc caatacgggt gaaaatgcgg
      601 tcaatcgggc cgatttccgc actttctgcc ggcacaaaac tgccgatata agccatcagc
      661 gtaatcaggg caatttgtcg catataggtg cttttaccgc ccatattcgg gccggttaca
      721 atcaataaat gccgttgggc atttaaaaac accggattgg caataaacgg ttcggttaaa
      781 acctgctcca ccaccgggtg tctgccgcct ttgatattca ccattcgcat cgggctaaag
      841 ctcgggcaaa catagcctaa actatcagct cgctcggcaa ggttggttaa gacatcaagt
      901 tcagacagca tcatcgccgc taattgcaat tcgcctaagc gaggcattaa cacatcaaac
      961 agttcatcat aaagctgttt ttcaagggca agcgatgccc cttttgcttt cagcactttg
     1021 tcttcgtaag ttttcagctc agggataata taccgctccg catttttgag cgtttggcgg
     1081 cgaacatagt gaatcggggc tttgtgtgct tgcccttggc tgatctgaat gtaatagccg
     1141 tgaaccgcat taaagccgat tttcagcgaa tcaatgccgg tgctttcacg ttctctgatc
     1201 tccaaatttt ccagatattg cgttgccccg tccgaaagtt gccgccattc gtcaagctcg
     1261 gcattataac cttctgcaat caccccacca tcacgaatga gttgtggtgg gttttcgata
     1321 atcgcccgct ccagcaaatc gtgtaacggg ctgaaatccg ccagttgagc caaaagctga
     1381 tcgatactag cggtcagatt ttgtgaattt tttgcaattt ccggtaactg agccaatgca
     1441 gtgcgtagtc gggtgaggtc acgagggcga gccgtacgca acgccactcg ggcgagaatc
     1501 cgctccatat cgccgacttg ttgtaataac ggctgtaaat ccgacattcg ctcatattgt
     1561 atcaatgctg ttatcgcctt ttgtcgggct tgtaatttac cacaatctcg aatcggctga
     1621 tgaatccaac gcttgagcag acggctcccc attggggtca cacatttatc taacaccgcc
     1681 gccagcgtgt tttccgtgcc acccgctaag ttttgggtca gctctaaatt gcgacgagta
     1741 gctgcatcga gcaaaatagt atcgctatgt tgtgccaaat gaattgcgtt aatgtgcggt
     1801 aaagcggtgc gttgcgtttc ttgggcataa tgcaagacac agccggcagc acaaagtgcc
     1861 accaccgctt tttccacccc aaatccggca agtgtttgcg tgccgaattg gcgatttagc
     1921 agctgaatcg ccgttaccag ctcaaattcc cacaccggac ggcggcgtaa ccctttgtaa
     1981 ttcgttaaaa ttgaggcagc ggaaaaatct tccgcataca gaatttctgc cggttgtaca
     2041 cgttgtaact cggcggatag agcctctttg ctagcaagtt cactgattaa aaaccgcccg
     2101 gaggtcatat ctagcgtagc aatcgcaaat gtgccttttt cttcgtaaac tgccgccacc
     2161 aagttatctt ggcgttcggg taacaatgcc tcatcgctaa ctgttcccgg cgtaacaatc
     2221 cgtaccactt tgcgttctac cggccctttt gaggttgctg ggtcgccaat ctgttcacaa
     2281 attgctaccg attcccccaa cgccaccaat ttcgccaaat acccctccac tgcgtgataa
     2341 ggcacacccg ccatcggaat cggctcaccg gcagattgcc ctcgcttggt gagcgaaata
     2401 tccaacaatg ctgccgctcg tttcgcatca tcataaaaca gctcataaaa atcccccatt
     2461 cggtaaaaca atagtatgtc cggattttga gctttgagtt gcaaatattg ttgcatcatt
     2521 ggcgtatggg tggagaaatc ttgtgtcatc agtctattta ttgtgagaaa tcattgctga
     2581 tagtttaaca gattcagcca agggataaaa acaagcggtc atttttcgca gaaaatttgc
     2641 aatggtcagc cgctgttttt ttgttacaat gcaccaaatt tttaaaacag aggtcagaat
     2701 gttaagaaaa atagtgctac tttctacagg cttattattg gcggcttgta gcagcaatga
     2761 accgcaacaa ccggaagcat tcccgggcga atttgccaat gccgattatg tgctttcgga
     2821 agaaaatgct caaaaatggg taattgccag cgaacaggca aaacagtgta tctaccccaa
     2881 tttaacccgt attcagcaag agcatttttc caaagaagat tcttatattt tctcacaata
     2941 tgtatttttc tatccgcttg aagagattat cggggagcag tatgtaaaaa tcattcaaaa
     3001 tgatgaaaaa tcaatgggtt acgctcaata tttattcaaa aaactgaaaa ataacgaagc
     3061 ggttgaagcg ttaccgaaaa aacaatgtgc gaccttacgc accaatgcaa aaaatgattt
     3121 ggcagtggta aaagggcagt atcaaagcgg tatgatcgaa gaaaccaata gccaagaagg
     3181 gaaatcaggc gataaagttg ccaccaatga taataaattc ttcttcgata ttatcaaatg
     3241 gggcagtatg ttacttctct agtgagcctc aatactcggt tgttttaacc acaaccgagc
     3301 catttatacc taccgattga acttttatct aaagcctcag tcttaaaata cagttttttt
     3361 caattatttt ccctctttta tatttaagga ttttcaataa aagcattaaa aacattatta
     3421 gcattgggcg tggcaatggc tgtttcagct acttcatttg cacaaaccac tcaacctact
     3481 gcggctgaac aaatgaacgc agttaaagcc tctgttgccg aaaaagcaac tgtggctaaa
     3541 gaagcaacaa aagagaaagc agcggagtta aaagccggtt ctgcggaaaa aatggcgaca
     3601 gcaaaagcca aaaccgaatc atttaaagat tccgctaaaa acaaaatgga cgcagtaaaa
     3661 gccaccacag cagaaaaaac ggacgtgatt aaagcgaaag ccgcatctgc aagcgacaca
     3721 atgaaagtga aagctcagga agcaaaagcc gccaccgcag agaaagctgc aactgctcat
     3781 gaaaccttaa attcagccaa aaccgcaacc gctgaaaaag cggccccaat gaaagcagct
     3841 gcaacagaaa aaatgtcagc agtgaaaaaa tcacaaaaag tgaatatcaa cactgccaac
     3901 gaagagcaat tacaatcgct taccggcatt ggtgaagcaa aagccaaagc gattgtagaa
     3961 taccgcaaaa aacacggtaa aatcaaaaat gcagcagaac tggcgaacat tcccggcatt
     4021 ggcgatgcaa ccattgagaa agttgctcca tatttaactt tctaatgttc tgcaagcggt
     4081 tattttgttg caaaattttg cagaaaaata accgcttgtt catttcacac cttgtattct
     4141 taaatttcac ccccattaag tgaatcgtat ttctttcaat acagtattca ataaactcaa
     4201 aaggaacaga aaatggcatt agatttcaat tcagttggat tagattcagt agaagcaaaa
     4261 ggcggttacg gcatcggttt acaaattggt cagcaacttt taggcagcgg aatggacgta
     4321 aacgcagaag ccgttgcccg tggtatttat gatgtgttaa acaacaatga gccggcaatc
     4381 gacatcaatg aagtgaccgc tgcattacaa gaattaggtc agcgtgcgga agcggctcaa
     4441 gcagaagcat tcaaagcgat tgatgccgaa aacaaagcgt tcttggaaga gaataaaaaa
     4501 gcaaacggtg taatcgttac cgacagcggt ttacaatatg aaattttaac cgaaggcaca
     4561 ggcgaaaaac cgactgcgac ttcaaccgtg cgtgttcact acaccggttc attaattgac
     4621 ggcaccgtgt ttgacagctc ggtaaaacgc ggtcagccgg cagaattccc ggtaaacgga
     4681 gtaattcgtg gttggacaga agcattgcaa ttaatgccgg ttggctcaaa atggcgttta
     4741 accattccac aagaattagc ctacggcgaa cgtggtgcag gagcatcaat tccaccattt
     4801 gcgaccttag tgtttgaagt ggaattgctt gatattctct aatcaaactc aaataaaaaa
     4861 cctgtgaagg catttcacag gtttttgcta tttaagataa ctatattatg cagaaaaaat
     4921 cactcatatc tcgcttactt aaaaatattt tcctctacgg cagcctgttt atcattatta
     4981 gcctggtggt tgattggtat cgctcgccct ccgctccggc acaatttgag caaaccgtgc
     5041 attatgatat tcaacaccag ccgaaagtaa tcgcccaatt aagccacgag aagccgatgc
     5101 tactctattt ttggggaagt tggtgccact attgccaagt ggtttcgccg aatatccaac
     5161 aattagcgaa tgacggcacg gaagttttag gtgttgccct gaaatccggc gatgaaaatg
     5221 acgtacaagc ctatctcaat gaaaatggct acactttcgc tactttgaat gacccaagcg
     5281 gtgatttctc tcgaggttgg aacattcaag ccaccccaac catcttgatt atcaaagacg
     5341 gtaaaatcgc ccaccacacc accggctaca ctagctattt gagcttaaaa ttcagactat
     5401 ggctggcaga cgtgttgtat taaacgattc ctttcatttg caaaaaaatc cactaaatcc
     5461 gaccgcttgt ttacaccaaa aagacaagcg gtactaaatt cattgtcatc attgtggtga
     5521 tcgataaaat cagcctcgat gagccaaaaa taaagcctcg gtttttgccg ttgtcgaatt
     5581 tcaccagtaa atttgccatt gctgtgaggg cgtacacttc caccaccgta aaaatcacgg
     5641 tgtaaatata ggcagcggtc agagttgcga cttttaccgc aataaaccaa ggggcaataa
     5701 tcgccaccgc taataacggc gaaatccaaa ttaattgctg tttggtgagg ttgattttac
     5761 gagaaaaagt cgattgcaaa ctgacggtta aaacaccgtt gatgaataac gcaatcggcg
     5821 aatgctccgg tgcgtgcagg acattgtcga ataaatacgg aaaaataata aaataggacg
     5881 atgccacact cagtggcaca aatagcatta agtgagcgaa caaaaactct ttgttgatga
     5941 tttccttaat ttgtgcgaaa cgaaatttca cgccgccatt cacgccttcc accgaataaa
     6001 acggcttgac cataaacgca aacaagccgg cttccaaaat aaagcagatc caaatcagcg
     6061 tttcaatcat ctcaaaccta ataaacggaa taataatcag cggggcgagc attgaagaca
     6121 tactggtgac ttttagatat ttgccttgta aacgggtttt agcagcatat tcatcgccgg
     6181 caatcgccag taaacaggct ctggcattag tgccgaataa gcaactcccc aacccaaagc
     6241 aagtggttgc caacaataaa atgaggtaat tatccgctgc taaaaacagc atataggcaa
     6301 ccacatctaa taaacagccc aataacatca tttttgccaa gccgtagcga tccccccaaa
     6361 tgccggctaa aattgccaat gcctgattac aaaacaacag caatgaaagg aaaaaagcga
     6421 tttgggcgtt atctagctta aataaaagaa aaacacgctt tgcattgaaa aaaaggcggt
     6481 ggtggaaata aaacggcgta ggagtaatag tttttctaaa gacattcaga atggttgaat
     6541 tggttaaata aagagcggtt attctacctt ttaagtcaac cccaatcaat cggcttttgt
     6601 tttacctttg gcgtgggttt cggtatgata agcgatatgt agtggcgggt cctgtgcccg
     6661 cctaaatacc aatgataact tgcgggcgag cacaggactc gcccctacga taaacaagaa
     6721 aatgaaagac accattgttg ctcaagcaac accgattggc agaggcggtg tggggattct
     6781 gcgagtttcc ggtccgcttg ccgaaaccgt tgctcaagcc gtattaggca aaacattgcc
     6841 accccgtatc gcaaattatt tgccgtttaa agacgaagat ggcactgtgt tggatcaagg
     6901 catcgcctta ttttttaaag ctcccaactc ctttaccggc gaagatgtgc ttgaacttca
     6961 aggacacggt gggcaggtta ttttagattt actgctcaaa cgcattttac aagttaaggg
     7021 gctacgcatt gcccgagcag gtgaattttc cgaacaggca tttttaaacg ataagctcga
     7081 ccttgcccaa gctgaagcga ttgcggattt aatcgacgcc accagcgagc aagccgctcg
     7141 ttcagccctg aaatcactac agggcgaatt ttctaataaa atcaatgcgt tagttgattc
     7201 agtaatttat ttacgcactt atgtggaagc ggcgattgac ttccccgatg aggaaattga
     7261 ctttctggca gacggtaaaa tcgaggcaaa attaaatgag attatcgctc aattagacgg
     7321 tgttcgccgt gaagcgaaac aaggctcgat tctgcgtgaa ggaatgaaag tagtgatcgc
     7381 cggtcgcccg aatgcaggga aatcaagctt gcttaacgcc ttagccggac gagaggcggc
     7441 gattgtaacc aatattgctg gcacaacccg tgatgtgttg cgtgaacata ttcacattga
     7501 cggaatgccg ttgcatatta tcgacaccgc cggtctccgt gatgccagtg atgaggtgga
     7561 acgtatcggc attcagcgtg cttgggaaga aatcgcccaa gccgaccacg ttttattaat
     7621 gattgacagc accgagcaac aagccgatca gttccgacaa gagtgggcgg aatttctagc
     7681 caaactgccg gcaaatatgc ctgtgaccgt aattcgcaac aaagtcgatt tatccggcga
     7741 gcaggaaggc ttaattcagg tggacgattt cacgatgatt cgcctctctg cccaaaccaa
     7801 agtcggcgtt gatttattgc gtgaacactt gaagaaatca atgggctacc aaagttctac
     7861 ggaaggcgga ttcttggctc gccgccgtca tctgcaagca ttggaaaccg ctgccgagca
     7921 tttagagcga gggcatatcc agcttactca attcttagcc ggcgaattgt tagcggaaga
     7981 gttgcgtatg gtgcaaaatg ccttaagtga aattaccggc cagttcacct ctgatgactt
     8041 attaggcaac attttcagct cattttgcat cggtaaataa atgacacttt ttaaccgctt
     8101 tcaaacccag tgtgcaacgc atttgccgaa ccaaaccgcc tttttaatcg gcttaagcgg
     8161 tggggtggat tcggtggtac tactccacct ttttgcccgc acctcatttc acgttagggc
     8221 aatttatgtc caccacgggc taagcccaaa tgcggacgac tgggcagcct tttgcgaaca
     8281 atattgcaag cggttaaata ttccctttat tttacaaaag gtcagagtgg atagctcaaa
     8341 cggcgttgaa aacggggcga gagaagctcg ttaccaagcc attcaacaac agcttaagcc
     8401 caatgaagtg ttcgccaccg cccaccattt agatgaccaa gccgaaactt ttttgctcgc
     8461 cctcaagcga ggcagtggag tcaaaggatt atcggcgatg caagcggtca cttttttgca
     8521 aaattttacc gtttttcgac cgctactcac ttttactaag tcagatttga tgggttatgc
     8581 cgttcaacat cagctaggtt ggattgagga cgaaagcaat gccgataacc gttacgaccg
     8641 taatttcctt cgcaatagca tcttgccact actcaatcaa cgttggcagc acttcagcca
     8701 aatggttgcc cgttccgctc agcattgtgc agaacagcaa gccttgatag aagagctatt
     8761 aagcgatgaa ctcaagtcaa gaacagggga aaaacaacaa ctcaacatta acggctttgg
     8821 gcaattttca cttgccaagc aacagcaact tagccgcctt tggctggaac aaaacggagt
     8881 gagaatgcca agccaagccc agctacaagc ggtcatttct gaactgattt ttgcaaaagc
     8941 cgataaaaac ccacaggtta aagtcggcga aaatattatc agacgctatc aacaagctat
     9001 ttacgttacc gaagagatcg ccgacattcc gccatttgag gtagtacttc ccacagaaac
     9061 caaagtggaa ttactctatc agctcggtac tattattcgc caacaacaag agatcatttg
     9121 caaaaaaaac gggaaaaatc accgcttgca actgccccaa gagctggctc aatcaactat
     9181 ttctattaaa atcgggcaac taggcaaagt aaaatgctac ggcaaggctc aacgggaaga
     9241 aatgaaaaag atttggcaaa atcacggcat acctgtttgg gaacgcaacc gtacaccgct
     9301 gattttttgg caagacgaat tagttggcct attacctatc ctctagggtg aaattgctga
     9361 tgaagcgatt tcaaacgggc ttttgccact tgagtgtaaa tctgggtggt cgataaatca
     9421 ctatgcccca gcaacatctg taccactcgc aaatcagccc catgattgac caaatgggtc
     9481 gcaaaggcgt ggcgaagcac gtgaggcgag agtttatctg catcaatacc tgcaagcacg
     9541 gcatagtgct taatccgatg ccaaaacgtc tgccttgtca tctgctgccc tcgccggctc
     9601 ggaaatacaa catcagactg ctgattattc agcaaaatag ggcgaccgta ctggaaaaac
     9661 tcctgtatcc aataattcgc ctcctcgccc aatggcacta accgctcttt atccccttta
     9721 ccgataattc tcaccccccc ttgtttgagg ctgatattat ccatcgaaag cgacaccagc
     9781 tccgttaccc gcagaccggt ggcatacagc agctccagca ttgctttatc ccgtaactct
     9841 atcggatcta acgtattcgg ggcatctagc aaatccatta cctgatcttc acttaaggat
     9901 ttgggtaaag aagcaccccg tttaggggaa gaaagggtta aagtcgggtc atcttggcga
     9961 taatgctcaa ggcataaaaa acggaaaaat ttccttaaac aactcaacat tctggcagag
    10021 cttgtcgcct tatagccttg ctccaaacgc tcgccgagaa aaccttgtaa gtcgatatga
    10081 tcgagagaca gaaatgcttt aggttcagaa aaataatcgg aaaaacgctc taagtcagaa
    10141 cgataagagg caacggtatt ttcagacaaa ccgtgttctt gccatagtgt atcgagaaat
    10201 tgttcaataa tagggtcaag ctcagccata caagcgggta tattttcagg aaattttgca
    10261 aattttaaca gaattaaatc tataaaaata caaaagcgat agaaaagaaa aaaccgatta
    10321 gcttatgcta atcggttta
//
LOCUS       DANJWC010000079        10331 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.079, whole genome shotgun sequence.
ACCESSION   DANJWC010000079 DANJWC010000000
VERSION     DANJWC010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10331
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.079"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            34..822
                     /locus_tag="PWX02_001442"
     CDS             34..822
                     /locus_tag="PWX02_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1729447.1"
                     /translation="MDILDRLLAFAQISAGISVKCQLQGDIVFFPRFADHTLRSQIQP
                     LTSKNAVNLEQLGAFTLTQAGLSQSKCDMFCLHFYYDHQAELMESLPEMIVFQVAETP
                     LKAMLDILKQEAETPNLAVKSVVNSLAVVILTMILREHLLVNHNNQLFGILKGWQDLR
                     LRPLIKAILLNPEHEWQVDDMAAFIHLSRAQLVRLFNKHLCISPHAFVHKIRLQKAAM
                     LLKSQAGSVLSIALSCGFQSEPHFITAFKKQYCVTPSQYRQLNP"
     gene            1020..1583
                     /locus_tag="PWX02_001443"
     CDS             1020..1583
                     /locus_tag="PWX02_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HDL1729448.1"
                     /translation="MSAPNSGTNPAPLGLCGFALTTFLLSLINNGTFSGENVGLVLAM
                     GFAFGGTAQMIAGMFEFAKGNTFGFTAFTSYGAFWWSFALFKVFFAANVSAEFVGWYL
                     VAWGTFTLMMFIGTLAKARALQAIFLSLTITFYLLAAGDFTHNTTLTNIGGNFGLLTA
                     FLAFYLAAADIINESFGKTVLPIGEPK"
     gene            complement(1688..4123)
                     /gene="plsB"
                     /locus_tag="PWX02_001444"
     CDS             complement(1688..4123)
                     /gene="plsB"
                     /locus_tag="PWX02_001444"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HDL1729449.1"
                     /translation="MSGLLNFYRAFLNLPLSLLVKSRSIPTHPVAELELNLEQPIVYV
                     LPYTSQTDLLILQKNCLALNLPDPLQENVIEGQTLLRFVFLDEGRRFFKSKGAKSETE
                     SIFYRYLDLHRANAELDVQLVPVSVLWGRAPGKEDARHLQVLTSFQRFLSMVWFGRDN
                     FVRFSPAVSLRYMVTEYGADEKIAQKIARVAKMHFAKLRYSAMGPRLPNRDAMFNKIL
                     NSEVIQAAIAEEAKKSSPEKARKEAEKIINEIAADVKHESLRVADRVLSWLWNKLYQG
                     INVQNADRVRKLALEGHEIVYVPCHRSHMDYLLLSYLLYHQGLVPPHIAAGINLNFWP
                     AGPIFRSWGAFFIRRTFKGNRLYSTIFREYLAELFYRGYSVEYFIEGGRSRTGRLLEP
                     KTGMMSMSLQALQRGLNRPLSIVPVYIGYEHVLEVDTYAKELRGAAKEKENAGLVLRV
                     LKKLKNLGQGYVNFGEPILVNNYLNQYFPEWKEPSEDGRPKWLNEAVDNISHQVMVNI
                     NKAAAVNAKNLVGSALLASRQRALTREQLIEQLESYIQLFRNVPYSKDMTLPTESAEV
                     MLNHVIHLPRSGVLIEKDNFGELVRLERESAVLMTYYRNNVQHLFVLPSLVASMVLHH
                     EAVSKDVVIKSVNRIYPFLQAELFLHFKQEELKAHIEAIIAELSRQGVIKNESDMLMI
                     NRSHIRTLQLHSAGVREILQRYYISLSVLLEQPNISRGSLEKESSSIAQRLSVLHGIN
                     APEFFDKAIFSTFTSSLKAQGYFDGDDNLAIAKVKETESLLRSLISIEVQQTIQGAMA
                     KVAESVENPNIAG"
     gene            4290..4925
                     /gene="lexA"
                     /locus_tag="PWX02_001445"
     CDS             4290..4925
                     /gene="lexA"
                     /locus_tag="PWX02_001445"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HDL1729450.1"
                     /translation="MRKHLTARQQEIFDFVKNHIETTGMPPTRVEIAREIGFKSPNAA
                     EEHLKALARKGYIEMLSGTSRGIRILVEDENQAANDEDEGLPLIGKVAAGTPILAVEH
                     VENHYPVNGAMFNPSADYLLKVNGNSMEKIGILDGDLLAVHKTNTARNGQVVVARVND
                     EVTVKRLEKKGELIYLHPENDELKPIVVNPAQEYIEIEGIAVGVIRSNAWM"
     gene            5017..5697
                     /gene="mutH"
                     /locus_tag="PWX02_001446"
     CDS             5017..5697
                     /gene="mutH"
                     /locus_tag="PWX02_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HDL1729451.1"
                     /translation="MQLSTFSPTEQALLSKAEWLAGFTLGEIAEMLHIPIPADLKRDK
                     GWVGTLIETALGAKAGSKAEQDFAHLGIELKTIPVNQKGLPLETTFVSLAPLTQNNGI
                     TWETSHVKHKLSRVLWIPVEGERKIPLAERHIGQPILWSPTITQESRLQQDWEELMEL
                     IVLGELHKINATLGEVLQLRPKGRNNRSITSAINAKGEIVQSLPLGFYLRKHFTAEIL
                     QNFLHCPL"
     gene            5786..6517
                     /locus_tag="PWX02_001447"
     CDS             5786..6517
                     /locus_tag="PWX02_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDL1729452.1"
                     /translation="MFDWITNPEAWVALFTLTGLEIVLGIDNIIVISILVSRLPIEQR
                     QSARIIGLGLAMGTRILLLLSLAWMMRLVEPLFTIGNNPISGRDLILLIGGLFLIVKS
                     AMELKESISAESHEEKSESIKKASFFMVLIQIAIFDIIFSLDSVITAVAMADDIPVMV
                     IAIIIAVAVMMLAAKTIGDFVDNNPTIKNLALAFLILIGIVLVGEGFDIHIPKAVVYT
                     AMGFSVVVEMLNIKMRRNQAKLEQA"
     gene            6643..7680
                     /gene="nagZ"
                     /locus_tag="PWX02_001448"
     CDS             6643..7680
                     /gene="nagZ"
                     /locus_tag="PWX02_001448"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005711660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HDL1729453.1"
                     /translation="MLLIDIKDKELTQEEVEMLEHPLVSGLILFTRNFYDKAQIQALV
                     KSIRQRVKKPLLITVDQEGGRVQRFREGFTKLPAMQSFSLLAQTQEHALQLARETGWL
                     MAAEMFALDIDLSFAPVLDLGHECKAIGDRSFGKDPYTILPIAEAFIDGMLEMGMATT
                     GKHFPGHGYVLADSHLETPFDDRAKEVIFNHDILPFKQLISKGKLSAIMPAHVVYTEC
                     DNQPASGSIYWLKDILRKQLNFNGVIFSDDLGMRGASFMGNVVERTEKAIQAGCDLLL
                     LCNQPEGVVQVLDGLKYQPTQIQKARHLSLMKRKTVTWRELENSKAYQQAQARLTALQ
                     NHWLAWKETNA"
     gene            7805..8386
                     /locus_tag="PWX02_001449"
     CDS             7805..8386
                     /locus_tag="PWX02_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL1729454.1"
                     /translation="MQNKTEILPLVASPISHFRNKAKMVVSGSVERPILGILKDQHDP
                     QSGVDLTDCLLYPASFYSLFPILKDFIGRAGLVPYNIKKKKGELKYILITESQYNQSL
                     MLRFVVRSESKRALVERELPHLLEKLPPNSVVSLNIQPQHAAVLEGETEIFLTERKVI
                     EENFNSVPLFIRPQGFFQTNPTVASQLYLTAQQ"
     gene            8636..9172
                     /locus_tag="PWX02_001450"
     CDS             8636..9172
                     /locus_tag="PWX02_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005547254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HDL1729455.1"
                     /translation="MKMKTALAIALGSLFSFSVTAAELEKITVPIQLLDVEKGNKDIG
                     TVEITESKYGLVFTPNLKDLTEGLHGFHIHENPSCDAKEKGGKLTAGLAAGGHWNPNK
                     APHHGFPWSDDAHLGDLPALTVLHDGTSTNPVLAPRLKKLDEIKGRSLMIHEGGDNHS
                     DHPAPLGGGPRMACGVIK"
     gene            9342..9539
                     /gene="rumB"
                     /locus_tag="PWX02_001451"
                     /pseudo
     CDS             9342..9539
                     /gene="rumB"
                     /locus_tag="PWX02_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617603.1"
                     /note="RNA uridine methyltransferase B; catalyzes the
                     formation of 5-methyl-uridine at position 747 in 23S rRNA;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase"
     gene            9944..>10331
                     /locus_tag="PWX02_001452"
     CDS             9944..>10331
                     /locus_tag="PWX02_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729456.1"
                     /translation="MTEKERLKKYINDIENAAFFFETHFINKEVVYSTDDENISIIFK
                     RSNFMHLCGVKYDDGAREFFYAAINNRLDINKIKTKSDGTTFLKLSLLKSINFLLSSE
                     ITLTNNAIYLNIAFDKALKTKKQVFAL"
BASE COUNT         3222 a         2102 c         1934 g         3073 t
ORIGIN      
        1 cgcaatttaa ggtggaaata taaggaatga acaatggata tattagatag gcttctagct
       61 ttcgctcaaa tctcagccgg aatcagtgtg aaatgtcagt tacagggaga tattgtattt
      121 ttccccagat ttgccgatca tacattacgt agtcaaatac agcctttgac cagcaaaaat
      181 gcggtaaacc ttgaacaatt gggtgcattt acactcaccc aagcaggact ttctcaatca
      241 aaatgcgata tgttctgcct ccatttttat tatgaccatc aagccgaatt aatggaaagc
      301 ttacctgaaa tgatcgtatt tcaagttgcc gagacaccat taaaagcaat gttagatatt
      361 ctaaaacaag aagcagaaac acctaattta gccgtaaaat ctgtggttaa ctcgcttgct
      421 gttgttattt taactatgat cttacgagaa catcttctag ttaaccataa caatcagtta
      481 ttcggtattt taaaaggttg gcaagactta cgcttaagac ctttgatcaa agctattttg
      541 cttaatcccg aacacgaatg gcaagtcgat gatatggcgg catttatcca cctttctcgt
      601 gcccagttag tccgcttatt taacaaacat ttatgtatta gcccacatgc ttttgtacac
      661 aaaattaggc tacaaaaagc agcaatgctg ctcaaaagcc aagctgggtc ggtattaagt
      721 atcgccttat cttgcggctt ccaatccgag ccgcatttta ttaccgcctt taaaaagcaa
      781 tattgcgtaa cacccagcca atatcggcag ttaaatccat agctaaatga caacaacaag
      841 cggtcatttt ttggcaaaat tttgcaaaaa tctcaccgct tgtctcggaa atttctagtc
      901 aatttactgc aaaatcgcta tactttatcg ccctaaattt taggcacata acgtctgatt
      961 tatcggcgta gataaacaat taccacactt atggtgtaac aatataacag aggttttcaa
     1021 tgtcagctcc aaattcagga actaacccgg ctccgctcgg tttatgcggt tttgcactta
     1081 ctacattttt actaagccta atcaataacg gtacattcag cggtgaaaac gtaggtttag
     1141 ttttagcaat gggatttgct ttcggtggca cagcacaaat gattgcaggt atgtttgaat
     1201 ttgctaaagg taacacgttc ggctttactg cgtttaccag ctatggtgca ttctggtggt
     1261 cttttgcatt atttaaagtg ttctttgctg cgaatgtatc ggcagaattc gttggttggt
     1321 acttagtagc ttggggaaca ttcacactta tgatgttcat cggcacacta gcaaaagccc
     1381 gtgcattaca agctattttc ttaagtttaa ccattacttt ctacctttta gccgcaggtg
     1441 attttaccca taacaccacc ctaactaaca ttggtggtaa cttcggttta ttaaccgcat
     1501 tcttagcgtt ctacttagcc gctgcggata ttattaatga aagttttggt aagactgttt
     1561 taccaatcgg tgaaccaaaa taattatata ggaagtaaaa aagctgaatg tggtcgcgca
     1621 ttcagctttt atttttggca acaaaattgc aagaaacgag cgcttgttag cgcccttctt
     1681 tgatttatta tcctgcgata ttaggattct ctacagattc tgcgacttta gccatcgcac
     1741 cttgaatggt ctgttgaact tcaatcgaaa tcaaactacg taacaacgat tccgtttctt
     1801 taacttttgc aatcgctaaa ttatcatcac catcaaaata cccttgtgct tttagacttg
     1861 atgtaaaggt agagaaaatc gccttatcaa aaaactccgg cgcattaata ccatgcaaga
     1921 ctgataaacg ctgagcaatt gaactactct ctttttctaa agagccacga ctaatatttg
     1981 gctgctccaa caagacactt aggctaatat aataacgttg aaggatttca cgcacaccag
     2041 ctgaatgtaa ttgcaaggta cgaatatgtg agcgattgat cattaacata tcactttcgt
     2101 ttttaatcac cccctgccga cttagttccg caataattgc ttcaatatgt gcttttaatt
     2161 cttcttgctt aaagtgtaag aataactctg cttgcaagaa tggataaata cgattaaccg
     2221 acttaatcac aacatctttc gaaacagcct catgatgaag taccatactt gcaacaagtg
     2281 aaggcaacac aaataaatgc tgcacattat ttctgtaata tgtcattaac acagcagatt
     2341 ctcgttctaa acgaaccagt tcaccaaaat tatctttttc aattaaaacg cccgaacgag
     2401 gtaaatgaat aacatgattc aacatcactt ctgcactttc agttggtaat gtcatatctt
     2461 tagaataagg tacattacga aataactgaa tataactttc aagttgttca atgagttgtt
     2521 cacgggttaa tgcacgctgg cgagatgcca ataatgctga ccccactaag tttttcgcat
     2581 taactgctgc cgctttattg atattcacca ttacttgatg tgaaatatta tccacggctt
     2641 catttaacca cttaggtcgt ccatcttcgg aaggctcttt ccactcaggg aaatattgat
     2701 ttaagtaatt attcaccaaa attggctcac cgaaattcac atatccttga cctaaatttt
     2761 tcagtttttt cagcactcgt agcaccaagc ccgcattttc tttctctttt gccgcacctc
     2821 tcaactcttt agcgtaagta tcgacttcta agacgtgttc ataaccgata taaacaggca
     2881 caatacttaa aggtctattc agccctcgct gtaaggcttg taggctcatc gacatcatac
     2941 cggttttagg ctctaataaa cgacctgtgc gtgaacgccc gccttcaata aaatattcga
     3001 ccgaataacc acgatagaat aattccgcta aatattcacg gaaaatggtg gaatagaggc
     3061 gatttccttt aaaggtacgg cgaatgaaaa atgctcccca actacggaaa atcgggcctg
     3121 ccggccagaa atttaaattg atcccagcgg caatatgtgg cggtactaat ccttgatggt
     3181 aaagtagata agataggagc aaatagtcca tatggctacg gtggcaaggc acataaacga
     3241 tctcgtgacc ttctaatgcc aatttacgca cacggtctgc attttgcacg ttgatccctt
     3301 gataaagctt attccaaagc cagctcagaa ctcgatctgc tacacgcaaa ctctcgtgtt
     3361 ttacatcagc ggcaatttca ttaatgattt tctcggcttc tttgcgagcc ttttcaggcg
     3421 atgatttctt cgcttcttcc gcaatcgcag cttgaatcac ctctgaattc aggattttat
     3481 taaacatcgc atcacgattt ggtaaacgag gccccattgc tgaataacgc aattttgcaa
     3541 agtgcatttt cgccacacgc gcgatttttt gagcgatttt ttcatctgca ccatattcag
     3601 ttaccatata gcgtaacgat actgcagggg agaaacgtac aaaattatca cgcccaaacc
     3661 aaaccatgga taaaaaacgt tggaatgagg taagcacctg taaatggcgg gcatcttctt
     3721 tgcccggagc ccgcccccat aataccgaaa ccggcactaa ttggacgtct aattcagcat
     3781 tagcacgatg taaatctaaa taacgataaa aaatcgactc tgtctcactt ttcgcccctt
     3841 tagatttaaa aaaacggcga ccttcatcta aaaatacaaa acgaagcaat gtttgccctt
     3901 caatcacatt ttcttgcaac ggatcgggca aattcaaagc caagcagttt ttttgtaaaa
     3961 ttaataaatc ggtttgcgag gtataaggta gaacatagac aatcggctgt tcaagattta
     4021 gttccagttc agccaccgga tgagtcggaa tagagcgaga ttttactaaa agtgaaagtg
     4081 gtaagtttag gaaagctcga taaaaattta ataagccaga catataaata tccttttaga
     4141 atcgttattg ttggaggtct aacctctaat ttcttccatt ctaccataaa tttttatgga
     4201 atttcgtgaa attattagtt acaaaatcaa aaatactgta tatactatca atattttctg
     4261 tatgtaaaaa cagtgaggat taacttaaaa tgcgtaaaca tctaactgcc cgtcagcaag
     4321 aaatttttga tttcgtcaaa aaccatattg aaaccaccgg tatgccacca acccgagtag
     4381 aaatagcccg tgaaatcggc tttaaatcgc ctaatgcggc agaagaacac ttaaaagccc
     4441 ttgctcgtaa aggctatatt gaaatgttat caggtacatc tcgtggaatt cgcattttag
     4501 tcgaagacga aaaccaagct gccaacgatg aagatgaagg cttaccgttg attggcaaag
     4561 tagctgctgg cacacctatt ttagcggttg aacacgttga aaaccattac ccggtgaatg
     4621 gtgcgatgtt taatccaagt gctgattatt tactcaaagt aaatggcaat tcaatggaaa
     4681 aaatcggtat tttagacggt gacctattag ccgttcataa aaccaatacc gctcgtaacg
     4741 ggcaagtggt ggttgctcgg gtaaatgatg aagtgactgt taaacgctta gagaagaaag
     4801 gtgaattaat ttacttacac cctgaaaacg atgaattaaa accgattgta gtaaaccctg
     4861 ctcaagaata tattgaaatt gaaggcattg cagtaggcgt tattcgcagt aatgcttgga
     4921 tgtaatttcc tcacaagcgg ttatttttta gtaatttttt gcaaatttct gcctaaattt
     4981 gaccgcttgc cagtttagtc taattaacta agtgcaatgc aattatctac attctctcca
     5041 accgagcagg cattattaag caaagcggaa tggctggcag ggttcacttt aggtgaaatt
     5101 gccgagatgc ttcatattcc gataccagca gatctgaaac gagataaagg ctgggtcggt
     5161 actctgattg aaaccgcttt aggtgcgaaa gccggcagta aagccgaaca ggattttgca
     5221 catttaggca ttgagctgaa aaccattccg gtcaatcaga aaggattacc gctggaaacc
     5281 acattcgtca gccttgctcc tttaacccaa aacaacggta ttacttggga aacctcccac
     5341 gttaaacata agctttcccg tgtgctttgg attccggttg aaggggaacg aaagataccc
     5401 cttgccgaac gccatatcgg tcagccgatt ttatggtctc cgactatcac acaagaatcc
     5461 cgcctacagc aagattggga ggaattaatg gagttgattg tgttaggtga actgcataaa
     5521 attaatgcaa ccttaggcga agtgttacaa ctcagaccca aaggtagaaa taaccgctct
     5581 atcacctctg ctatcaacgc taaaggggag attgttcaat ctctccctct tggtttctat
     5641 ttgcgtaaac attttaccgc cgaaatttta caaaactttt tgcattgccc cctttaaatt
     5701 tgtcctcaca ctctataaga tgtcggcttt aattctattt gatagaaaat atcacttaat
     5761 cccttatttt taaaaggaca tttttatgtt tgactggatc acaaatcctg aagcttgggt
     5821 tgcgctcttt acacttaccg ggcttgaaat cgtattaggc attgataaca tcatcgtcat
     5881 cagtattctc gtttcccgtt tacctattga acaacgccaa tctgcccgaa ttatcggctt
     5941 ggggctggca atgggaaccc gtattttatt gcttctctcg cttgcttgga tgatgcgttt
     6001 ggtcgagcct cttttcacta ttggcaacaa tccaatttca ggtagagatc tcattctact
     6061 catcggtgga ttattcctga ttgttaaaag tgcaatggag ctgaaagaat ccatttctgc
     6121 cgaatctcac gaagaaaaaa gcgaatccat taaaaaagcc agctttttta tggtgttaat
     6181 tcaaattgca atttttgata ttattttctc ccttgattca gtcattactg ctgtggcaat
     6241 ggcagatgat attccggtaa tggttattgc gattatcatt gcagttgcgg taatgatgtt
     6301 agcagcgaaa accattgggg actttgtaga taacaaccca actattaaaa atctcgcttt
     6361 agcgttcttg attttaatcg gtattgtatt agtcggcgaa ggttttgata ttcatattcc
     6421 aaaagcagtg gtttatacgg caatgggctt ctcggttgtg gttgaaatgc tcaacataaa
     6481 aatgcgtcgt aaccaagcta aattagaaca agcataattg ctctgtctca tttatgccct
     6541 taatcattcg cccttgatca acatcaaggg ctttttaatg tagaataggc acaacaagcg
     6601 gtcatatctg ctcatttttt tgcaaatagc tcaacgaaaa ttatgttact gattgatatt
     6661 aaagataaag aactgactca agaagaagtc gaaatgctgg aacacccttt agtttccggg
     6721 ctaattttat tcacccgtaa tttctacgac aaagcacaaa ttcaggcatt agtgaaatcc
     6781 attcgccagc gggtgaaaaa accgttgctt attactgtcg accaagaagg cggtcgggtg
     6841 caacgcttcc gtgaaggctt tacaaaattg cctgcaatgc agtcttttag cctgctagct
     6901 caaacccaag agcacgcatt gcaactcgcg agagaaacag gctggttaat ggcggctgaa
     6961 atgtttgcat tggatattga tttaagtttt gcccctgttt tagatttagg acacgaatgc
     7021 aaggcaattg gcgatcgatc atttgggaaa gatccataca cgattttgcc aattgctgaa
     7081 gcctttattg acggaatgtt ggaaatgggg atggcaacta caggcaagca ttttcctggt
     7141 cacggatatg ttttagctga ttcacattta gaaacgcctt ttgatgatag agcaaaagag
     7201 gtgatattta atcacgatat tctccccttc aaacaactta ttagtaaagg taaactctcc
     7261 gctattatgc cagctcacgt tgtttatacc gaatgcgata accagcctgc aagcggttca
     7321 atttactggt taaaagacat tttacgcaaa caactcaatt tcaatggtgt cattttctct
     7381 gatgatttgg gtatgcgagg tgctagcttt atgggtaatg ttgtagaacg caccgaaaaa
     7441 gccattcaag ccggctgcga tttattgctg ctttgcaatc agccagaggg cgtcgttcag
     7501 gttttagatg gcttgaaata tcagcctact caaattcaaa aagcgcgtca tttatcatta
     7561 atgaagcgta aaactgtcac ttggcgtgag ctagaaaaca gtaaagctta tcagcaagct
     7621 caagctaggc tgaccgcttt acaaaatcac tggctagcgt ggaaagaaac aaatgcctaa
     7681 tctagctaac tgctcacatt ttaaacgtgg tgattgcacc tcctgccaat gacttgaaaa
     7741 accataccag agccaattag ccgataaaga agccgacctc aagcggttaa tttctccatt
     7801 tattttgcaa aataaaacgg aaatcttacc gcttgtagca tcccccattt cacattttcg
     7861 caataaagcg aaaatggtgg tttcaggtag tgtggaacgc ccaattttag gcattttaaa
     7921 agatcaacac gacccgcaaa gtggggtaga tttaaccgac tgcctacttt acccagcttc
     7981 tttttatagc ttattcccca ttcttaaaga ttttattggc cgagcaggcc ttgtgcccta
     8041 caacatcaaa aagaaaaaag gcgagctgaa atatatccta atcactgaaa gccaatataa
     8101 ccaatcgtta atgctccgtt ttgtggtgcg aagcgaatca aagcgagcat tggtggagcg
     8161 agaattaccc catctgttag aaaaattacc gcctaattcg gtggttagcc taaatattca
     8221 accgcaacac gccgctgttt tagaaggtga aacggagatt ttcttaaccg aacgaaaagt
     8281 gatcgaggaa aatttcaata gcgtaccgct atttattcgc ccacaaggct ttttccaaac
     8341 aaatccaact gtagccagcc agctttactt gacagctcaa caatgagttg aaaatttacc
     8401 aataaaaaag tgttgggatc tgttttgtgg cgtaggcggt tttggtttac attgtacaaa
     8461 agcattacaa aaaaaaggat tgatgttgaa ttaactggca ttgaaatttc agcatccgca
     8521 attgaaagtg ccacaaaatc agcggcaaca ttagccttag aacttgacaa aatttgactg
     8581 ctatggttaa aatgaattgc caattaacgg cttatctatg aaaaagggca gtcttatgaa
     8641 aatgaaaaca gcattagcaa ttgcattagg ttctttattc agcttttcgg taacagcagc
     8701 cgaactggaa aaaattaccg tgccgattca acttcttgat gtagaaaaag gcaataaaga
     8761 tattggtaca gtagaaatta ctgaatctaa atacggctta gtatttactc caaatctaaa
     8821 agatttaaca gaaggcttac acggcttcca tattcacgaa aacccaagct gtgatgcaaa
     8881 agaaaaaggt ggcaagctca cagcaggctt agccgcagga ggacactgga accctaataa
     8941 agccccacat cacggcttcc cgtggtctga tgatgctcat ttaggcgatt tacctgcatt
     9001 aaccgtttta cacgatggta catcaactaa tccagtatta gcccctcgct tgaaaaaatt
     9061 agatgaaatt aaaggtcgtt cattaatgat ccacgaaggc ggcgataacc actcagacca
     9121 cccggctcct cttggtggtg gtcctcgaat ggcgtgtggt gtaattaaat aaaatttgat
     9181 ctagttagaa agtgttggtt taaatatcat tccaacactt ttttatttct agccattacc
     9241 atataattgc ctcctattaa ggagaaatat atgtctataa aagcaaaaaa agaaccccat
     9301 aaaatacttc cagaacaagc aactttttct aagagttcag agtaagattt atctcgctcg
     9361 attctgcaaa atttgcctta aatgaaaaca gcaccccacc tgatttggtg attgtaaacc
     9421 cacctcgtag aggcattggt caatctttag cggaattttt aaacaatttg ggctcaactt
     9481 atttaatcta ttccagctgc aatgcccaat ctatggcaaa agattttgaa tcgtttgtgt
     9541 aggattgttc aaatcatatt cgcaaataca cagttactac gatgtatcca gccaatacat
     9601 ccattgggat tttccgtccg aatttcagac gttctagatc ttacacattc tttcaagttt
     9661 gtaaagcctg aaaatcattt tttattcttg ctttttgtaa aaaaactccc tattatattg
     9721 ataaggaagc gtagccttaa aacgcaaatg tgggttagca catcgtcaat ctgaaactaa
     9781 tctaggatat ataaggccta gcagatggca agcatcagaa aaacttgaaa ctaatctagg
     9841 atatataagg cctagcagat ggcagacatc agaaaaacta acatctccaa acattttaag
     9901 cccacctttt ggtgggctta attatttcgg tggtataaat ataatgactg aaaaagaaag
     9961 attaaaaaaa tatataaatg atatagaaaa tgcggctttc ttctttgaaa ctcacttcat
    10021 taataaagaa gtcgtgtatt ctactgatga cgagaatatt tctattattt ttaagcgttc
    10081 aaattttatg catttatgtg gtgttaaata tgatgatgga gcacgagagt ttttttatgc
    10141 tgcaattaat aatagattag acataaacaa aattaagact aaatctgatg gaacaacatt
    10201 cctaaaattg tcattgttaa aatcaattaa ttttttacta agctctgaaa taactttgac
    10261 aaataatgct atttatctaa acattgcatt tgataaagca ttaaaaacta agaagcaggt
    10321 atttgcatta a
//
LOCUS       DANJWC010000080        10315 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.080, whole genome shotgun sequence.
ACCESSION   DANJWC010000080 DANJWC010000000
VERSION     DANJWC010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10315
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.080"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..1083)
                     /locus_tag="PWX02_001453"
     CDS             complement(<1..1083)
                     /locus_tag="PWX02_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL1729457.1"
                     /translation="MKSSFLLIGCILLSLVTAVIRLNMVWEWEWQLLLINVDLLPINQ
                     AAVKAALLPEMTTSFLAGGLLAIASSGLQHIVRNNLASDSTLAVSSGAQLALMIAVIF
                     FPAAELFSSFWIALLGSLAAIVLVLFIAKASKLNPLTLILGGLIVNILFGAIASLLTL
                     FYFDFLFGVMVWGSGSLLQDGWATSIILAITVVVAFFIFVLLSRPLTILSLDDEQARR
                     LGAPVNLLRYLVIFVCAAITALVVSKIGVIGFIGFAGASLAHFAKIRHLLLRLLIAFL
                     AGGFLLFFTNNLIGIVSRIYDTLLPVGAFTATLGAPLLIFLVLRQRKAQFEASNTAYF
                     PVKRLNSWKLPILLLCAGVILFCLLQI"
     gene            complement(1083..1964)
                     /locus_tag="PWX02_001454"
     CDS             complement(1083..1964)
                     /locus_tag="PWX02_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1729458.1"
                     /translation="MMFRQYKRFSFLFILQISTAFAQPNIFTPDWSIASALTEMGNPP
                     IAMGDKRIYPLWSHRPIQPNSVLDIGARYQPNRELLAQLPVELVLSNFFYAHLASVYP
                     KEIPQIDVLFDGGNTEPQQHWHTYQKATRMIGTEIKNPNAAEAYLTKTEQTLAQWGKE
                     ILNSNPDIDYYSVVQFGSPRELRIYASNSMFRVAFDLMGLKQADLGVGDRWGNKLIPV
                     SDLAKLPERTCLIVISPFSKMTQADLENSYIWQRLGFGKNRCMKVLPPVWLFGGADSI
                     SHFAEFLHNAMTQGEKE"
     gene            complement(1961..2749)
                     /locus_tag="PWX02_001455"
     CDS             complement(1961..2749)
                     /locus_tag="PWX02_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1729459.1"
                     /translation="MLLKHNTMIQINSISYSVEQNRKLLSNISLNLSKGRLYGVIGHN
                     GSGKSTLMRLLSGDITPTSGSILVDGLPLHQLSTKEAAKHIAYLPQRLPEAADFLVQE
                     LVALGRFPYQKWLQKPTEFDVQIVEQAMESTSVTQFAKQPVNSLSGGEKQRVWLAMCL
                     AQQTPYLLLDEPLAALDIVYQVEVMQLIRKLVNEQGLTVIIIIHDINLASQFCDEIIA
                     LKQGELCIQGSVDEIMQEQKLLEIFGLKLHLFNHPAGLHKVAVL"
     gene            complement(2981..4264)
                     /locus_tag="PWX02_001456"
     CDS             complement(2981..4264)
                     /locus_tag="PWX02_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1729460.1"
                     /translation="MTACHEPKRSIWQQIFTKNMLLCVYTGFCSGLPLFVLIQLMPAW
                     LTSANLDIKTIAAFTLTSLPYTWKFLWAALLDRYFPPFLGRRKSWIFISQIGLLVILA
                     SFGLFNPVQDISIIVTLSVLLAFLSATQDIVVDAFRREILSDNELGLGNSIHVNAYRI
                     AGLIPGGLSLFLADHYAWDTVFLITAAFLVPCLFVTLIIAAEPNHKPIDRSKPFYMAF
                     VDPFKEFFTRKGVAGAIGLILFIFLYKLGDSLATTLQTKFILDMGFTKSHIAGIVKIT
                     SLWCSIGGGIIGGVAMLKLGVNRALWLFGFVQLITILGFAYLASFEHFPTESIGAAEL
                     WKLGLVMASEYLGVGLGTAAFVAFMARETNPAYTAMQLAIFTSLAALPSKMLGAYTGN
                     LVEMMGYYQFFWLCFFIGIPGMLMLFKVAPWGQAE"
     gene            4430..8146
                     /gene="metH"
                     /locus_tag="PWX02_001457"
     CDS             4430..8146
                     /gene="metH"
                     /locus_tag="PWX02_001457"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL1729461.1"
                     /translation="MNTQHRTELLQNALTERILILDGAMGTMIQKYKLTESDFRGERF
                     KNSTIDLKGNNDLLTLTQPLLISAIHEKYLAAGADIIETNTFSATTIAQADYELESIA
                     YGLNLVGAKLARLAADKFSTPEKPRFVAGILGPTNRTCSISPDVNDPGFRNITFMQLV
                     EAYAEATRGLIEGGADLIMIETIFDTLNAKAAVFAIETVFDELGVKLPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAKPLTFGLNCALGPKELRPYVEMMSKISETFVSVHPNAG
                     LPNAFGGYDLGAEEMATYIKEWAELGWLNIVGGCCGTTPEHIQAFAEATKGVAPRKLP
                     EIPTAMRLSGLEPLTIDDNSLFVNVGERNNVTGSAKFKKLIKEEKFAEAIEIAISQVE
                     NGAQVIDVNMDEALLDSKKCMTRFLNILATEPEAAKVPIMIDSSKWEVIEAGLQTVQG
                     KPIVNSISLKEGEEKFIHQAKLCRRYGAAVVVMAFDEVGQADTEERKVEICTRAYRIL
                     VDQLGFPPEDIIFDPNIFAIATGIEEHNNYGVDFIQACERIKRDLPHAKISGGVSNVS
                     FSFRGNNVVREAIHAVFLYYAIKAGMDMGIVNAGQLAIYDDLDPELREAIEDAVLNHR
                     HDATDRLLEISEKYRGVKVESADESAAEWRNLPVAERLKHALVKGITTYIIEDTEEAR
                     QQFASPLEVIEGPLMAGMDVVGDLFGDGKMFLPQVVKSARVMKQSVAYLEPYINATKQ
                     KGSSNGKMVIATVKGDVHDIGKNIVSVVLQCNNFEVIDLGVMVPADVIIETAIREKAD
                     LIGLSGLITPSLDEMEYFLGEMNRLKLDIPVLIGGATTSKEHTAIKLYPKYNYPVVYT
                     ANASRCVTVCATLMNPEAKAEFWEKTKAEYEEIQRKFAVRKPLRNGLSIEEARANRFD
                     GFSGEWADYVPPTPKQTGIIEYKNVPIATLRKFIDWSPFFMLWGLMGGYPDAFDYPEG
                     GEEARRVYNDAQRVLDEFEQNGKLNPSGIMGIFPAVREGDDIAILNEDRTACIGKSYH
                     LRQQSERGKNSKSPYNLCLSDFIADRESGQQDWFGMFAVCAGIEEHYLVESYKAAGDD
                     YNAILLQAVGDRLAEAMAEYLHFELRTKVWGYTVEDEDNGRLIREEYIGIRPAPGYPS
                     CPEHTEKQIIWDLLEVEQRIGMKLTESYAMWPAASVCGWYFTHPASNYFTLGKIDEDQ
                     VLDYAKRKGWDELTAKKWLSVAMKQDVKSQAENI"
     gene            8254..10257
                     /locus_tag="PWX02_001458"
     CDS             8254..10257
                     /locus_tag="PWX02_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDL1729462.1"
                     /translation="MIEKLNYHLVQLLKDSNYQLTQFSPAYIKKLEESIFLKSGKNGD
                     IPHIQCLVRKKDIKLTPEEAVRQLYLMNLHYEYDYPFERMELEYSVVFGREKKRADIV
                     IFDRDNPNAVYIMVELKKPKLKDGKEQLKSYCNATGAPIGVWTNGESISYYNRRDPNY
                     FKDIPDIPNAQQKLSDLLQERWTIQDLIDNDKIAKQRKSLKDLVIEMEDEVLANAGVD
                     VFEELFQLIFTKLYDEMESTRNKSRYLEFRNYGDTESELKAKIQSLFDRAKNKWEGVF
                     AESAKINLSPSHLAICVSSLEEVKLFNSNLDVVDEAFEYLINKSSKGEKGQYFTPRYV
                     IDMCVKMLDPKPEETVIDTAAGSCGFPVHSIFHVWEKQLKARGLERSHLFTAEEKLPE
                     QTDYVKEKVFAIDFDEKAVRVARTLNLIAGDGQTNVLHLNTLDYERWEDFTKEEEWND
                     VYGEGWKKLRKLRKTKNENRDFQFDVLMANPPFAGDIKETRILARYELGKNSKGKQQS
                     KVGRDILFIERNLDFLKDGGRMAIVLPQGRFNNSSDKYIRDFIAERCRILAVVGLHGN
                     VFKPHTGTKTSVLFVQKWHDELCPKVEDYPIFFATMQKSSKDNSGDKIYVKNADGTLK
                     LDNHQHLIVEHDLFNHDNSTQDGIAEAFIEFAKKEKLSFVGKD"
BASE COUNT         3264 a         2115 c         2286 g         2650 t
ORIGIN      
        1 gatttgtaat agacaaaata ggataactcc agcgcataac aacaatattg gcagtttcca
       61 cgaattgagg cgtttaacgg ggaagtatgc tgtattgctt gcttcaaatt gtgctttacg
      121 ttggcgtaat accaagaaaa ttaataatgg tgcgcctagt gttgcggtaa atgcaccaac
      181 aggtagtaga gtgtcgtaaa ttctgctaac aatgccgatg agattattgg taaaaaagag
      241 taaaaatccg cccgctagaa aagcaatcaa gaggcgaagt aataaatgtc gtattttagc
      301 aaaatgagcc aaacttgcac ctgcaaatcc aataaagcca atcacaccta ttttgcttac
      361 aaccaatgct gtaatggcgg cacaaacaaa aataactaag tagcgtagta aattaacagg
      421 cgcgcctaag cggcgagctt gttcatcatc taaacttaaa attgtgagtg gacgagagag
      481 taagacaaag ataaaaaatg caactactac cgttatcgct aagataatag aagttgccca
      541 accatcttgt aaaagtgagc cacttcccca aaccatcacg ccaaataaaa aatcaaagta
      601 gaaaagggtc agtaaagagg caatagctcc aaacaaaata ttaacaatca atccccctaa
      661 aataagggta aggggattga gcttagaggc tttagcaata aataaaacca gtacgatagc
      721 agctaaagag ccaagtaacg caatccagaa actagaaaaa agctcggcgg ctgggaaaaa
      781 gataaccgca atcatcaatg caagttgagc cccgctactg acggcaagtg tgctatctga
      841 cgctaaatta tttctgacaa tatgttgtaa gccactgctg gctatagcca ataaaccgcc
      901 tgctaaaaat gacgttgtca tttcaggtaa aagagctgct tttaccgcag cttgattaat
      961 tggcaataaa tcaacattta tgagtagtaa ctgccattcc cactcccaga ccatatttaa
     1021 acggataaca gctgttacta aggataaaag aatacagcca atcaataaga atgatgattt
     1081 cattactctt tttctccttg tgtcattgca ttgtgtaaaa actcagcaaa gtgcgagatg
     1141 gaatcagccc caccaaagag ccaaacaggc ggtaatactt tcatacaacg gtttttacca
     1201 aatcctaaac gttgccaaat gtagctattt tctaaatctg cttgcgtcat tttagaaaaa
     1261 ggtgagatga caattaaaca ggtgcgttcg ggtaatttag ctaaatcact cacaggaata
     1321 agtttatttc cccagcgatc tcccacacct aaatcagctt gttttaagcc catcaaatca
     1381 aaggctacac ggaacattga attagacgca tatatgcgta actctctcgg agagccaaat
     1441 tgaacaacag agtagtaatc aatatctgga ttactgttaa ggatttcttt cccccattga
     1501 gccaacgttt gttcagtttt cgttaaatat gcttctgccg cattagggtt tttaatctct
     1561 gttccaatca tacgagtggc tttttggtaa gtatgccaat gttgctgagg ttcagtattg
     1621 ccaccatcaa atagcacgtc tatttgtgga atttctttcg ggtaaacaga agccaagtga
     1681 gcgtagaaaa agtttgatag caccaattca acaggaagct gtgctaataa ttcgcgatta
     1741 ggctgataac gcgctccaat atctaacacg ctattaggct gaatagggcg gtgcgaccat
     1801 agcggataaa ttcgcttatc ccccatcgca atgggcggat ttcccatttc agtaagggca
     1861 gaggcaatag accaatctgg tgtaaaaata ttaggttgag caaatgcggt agaaatttgc
     1921 aaaataaaga gaaaactgaa ccgcttgtat tgcctaaaca tcataatacc gccactttat
     1981 gtaagcctgc agggtggtta aacaagtgca gttttagccc gaagatctca agtaatttct
     2041 gttcttgcat aatttcatca acagaacctt gaatacagag ttcgccttgc tttaacgcaa
     2101 taatttcatc acaaaattgg gaggcaagat taatatcgtg aataatgata atgacagtta
     2161 gaccttgttc attcactagc ttgcgaataa gctgcattac ttcaacttga taaacaatat
     2221 caagggcagc caatggctca tctagcagta aatagggtgt ttgttgggct aagcacatag
     2281 caagccaaac tcgttgtttt tctccgcctg atagactatt tacaggctgt ttagcaaact
     2341 gagtaacgga ggtgctttcc attgcttgct caacaatttg aacatcaaat tctgttggtt
     2401 tttgtagcca tttttgatat ggaaatcgcc ctaaagcaac cagttcctgc actaagaaat
     2461 ccgccgcttc aggtaaacgt tgtggtaaat aggcaatatg ttttgccgct tctttggtag
     2521 aaagctgatg aagtggtaat ccatcgacta aaattgaacc gctggtaggc gttatatctc
     2581 cgcttagtaa acgcattaac gttgatttac ccgagccgtt atggcctatt acgccatata
     2641 aacgcccctt gcttaaattg agggaaatat tgcttagcag tttacggttt tgttcaacag
     2701 agtagctaat ggaatttatt tgaatcatag tgttgtgttt taagagcata aaaaaggtgg
     2761 gattacccca ccaaatcaat caaaatgaaa atgagtagat gaaaatctat tatgaaaacg
     2821 gtgctaaata tatttttatt ataaatgaaa gtcaatatca tttagtttta ggtgagggta
     2881 aattttagga taaataaaaa ataaccgact aaattactag gtaattcaat cggttataag
     2941 tggtcggata tttaataaaa aatcttattt ttatgaatag ctattcagct tgtccccaag
     3001 gagcaacctt aaacaacatt agcattcccg gaataccaat aaagaaacag agccagaaga
     3061 attggtaata gcccatcatt tccactaaat tccctgtgta tgcccctaac attttgcttg
     3121 gtagggcagc aaggctggtg aaaatggcga gctgcattgc agtgtaagcg ggattagttt
     3181 cacgagccat aaaggcaacg aaagccgctg tgcctaaacc cacgcccaga tattcacttg
     3241 ccatcactaa gcctaatttc caaagctcgg cagctccaat gctttcggta gggaaatgct
     3301 caaaactggc aaggtaagcg aagcctaaaa tagtaattaa ttgtacaaat ccgaacagcc
     3361 ataaggctcg gttaacgccc aatttcaaca ttgccacgcc accaataata ccgccgccaa
     3421 tgctgcacca aagtgaagtt attttcacaa tgccggcaat gtgcgattta gtaaaaccca
     3481 tatccaaaat aaatttggtt tgtagcgtag ttgcaaggga atcgcctaat ttatatagga
     3541 aaataaatag aatcagccca atcgctcctg ccacgccttt acgggtgaaa aactctttga
     3601 acggatcgac aaacgccata taaaacggct tagaacggtc gattggctta tgattcggct
     3661 cggcagcgat tattagcgta acgaataagc aaggcactaa aaaagcagcg gtaatcagaa
     3721 atacggtatc ccacgcatag tggtcggcta aaaataatga aagcccaccc ggaatcagcc
     3781 ctgcaatacg ataagcatta acgtggatag agttacctaa cccgagttcg ttatccgata
     3841 aaatttcacg ccgaaaggca tccaccacaa tgtcttgcgt ggcggaaaga aaagctaata
     3901 gcacagaaag agtaacaata attgaaatat cttgcactgg gttgaacaag ccaaagctgg
     3961 ctaaaatcac caatagcccg atttgtgaaa tgaaaatcca gcttttgcgt ctgcctaaaa
     4021 aaggtggaaa gtagcgatct aacaatgcag cccacaggaa tttccacgtg tagggcaaag
     4081 aggtcagggt gaaggcggca atagttttaa tatctaaatt ggcactggtt agccaagccg
     4141 gcattagctg aattaacaca aacaacggta aaccggaaca aaagccggtg taaacacaaa
     4201 gcaacatatt tttagtaaaa atctgttgcc agatagagcg tttgggttcg tgacaagcgg
     4261 tcatatttct cctacatttt gcaaaataaa aaggggaaga actgagccaa tatagcataa
     4321 ttttaaaggc gactctaacc ctttccattt cgtcacagat aaggtttgac agccttgcga
     4381 catatttcta ttattccttt taatcaaact aaaaaactgg aagcagagta tgaacacgca
     4441 gcatagaacc gaattacttc aaaatgccct taccgaacgc attcttatct tagacggtgc
     4501 gatgggaacg atgatccaaa aatataaact aaccgaatct gatttccgag gtgaacgttt
     4561 taaaaacagt accatcgatc ttaaaggcaa taacgaccta ctcacactca cccagccact
     4621 cctgatttct gctattcacg agaaatattt agccgccggt gcggatatta tcgaaactaa
     4681 tacctttagt gcgacgacca ttgctcaagc agattatgaa cttgaaagta ttgcttatgg
     4741 gctgaatctt gtgggggcaa aacttgcccg tcttgccgcc gataaattct ccacccctga
     4801 aaaacctcgt tttgttgccg gtattcttgg gccgactaac cgtacttgtt cgatttcccc
     4861 tgatgttaat gaccccggtt tccgtaacat tacctttatg caactggtgg aagcctatgc
     4921 cgaggcaacc cgtggtttga ttgaaggtgg ggcggatctg attatgatcg aaaccatttt
     4981 cgataccctg aatgccaaag cggcggtgtt tgcaattgaa acagtgtttg atgaactggg
     5041 cgtgaaattg ccgataatga tctccggcac cattaccgat gcctcagggc gaacgttgtc
     5101 agggcagacc acggaggcgt tttataactc gctccgccac gccaaaccgc tgacgttcgg
     5161 cttgaactgt gcgctcgggc cgaaggaact ccgcccttat gtagagatga tgtcgaaaat
     5221 cagtgagact tttgtttcgg ttcacccgaa tgccggcttg ccaaacgctt ttggcgggta
     5281 cgatttaggg gcggaagaga tggcgactta catcaaagag tgggcagaac tcggctggct
     5341 gaatattgtc ggcggctgtt gtggcaccac gccggagcat atccaagcct ttgccgaagc
     5401 aaccaaaggc gttgcgcctc gtaaattacc tgaaattcca accgctatgc gtttgtcggg
     5461 cttagaaccg ctgacgattg acgataattc gctgtttgtg aatgtgggcg aacgcaataa
     5521 cgtcaccggt tcggcaaaat ttaagaagct gattaaagaa gaaaaatttg cggaagcgat
     5581 tgaaattgcc atcagccaag tggaaaacgg ggcgcaggtg attgacgtga atatggacga
     5641 ggcgttgctc gattccaaaa aatgtatgac ccgtttcctc aacattcttg ctaccgaacc
     5701 tgaggcggca aaagtgccga ttatgattga ctcctcgaaa tgggaggtga tcgaggcggg
     5761 cttgcaaacg gtgcagggca agccgatagt gaactccatt tcactcaaag agggcgaaga
     5821 gaaatttatt caccaagcca aactctgccg ccgctatggg gctgcggtgg tggtgatggc
     5881 atttgacgaa gtcgggcaag cggataccga agaacgcaag gtagaaattt gtacccgagc
     5941 ttatcggatt ttggttgatc agctcggctt cccaccggaa gacattattt tcgacccgaa
     6001 catttttgcg attgccaccg gtattgaaga acacaataac tacggcgtgg attttatcca
     6061 agcctgtgag cggattaaac gagacttgcc acacgccaaa atttccggcg gggtgtcgaa
     6121 tgtctctttc tctttccgag gcaacaatgt ggtgcgtgag gctattcacg ccgtgttcct
     6181 ctactatgcg atcaaagccg gaatggatat ggggatcgtg aacgcagggc agttggcgat
     6241 ttatgacgac ctcgaccctg aactgcgaga ggcgattgaa gatgcggtgc tgaaccaccg
     6301 tcatgacgct accgaccgcc tgttggaaat ttccgaaaaa tatcggggcg taaaagtaga
     6361 aagtgccgat gaaagtgccg ccgagtggcg aaatttacct gtggcagagc gtttaaaaca
     6421 cgccctcgta aaaggcataa cgacctacat tattgaagat accgaagagg ctcgccagca
     6481 atttgcctct ccgctggaag tgattgaagg gccgctcatg gcgggtatgg acgtggtggg
     6541 cgacctgttc ggagacggca aaatgttcct gccgcaagtg gtgaaatcgg ctcgggtaat
     6601 gaaacagtcc gttgcctatt tagagccgta catcaacgcc accaagcaga aaggatcgag
     6661 caacggcaaa atggtgatcg ccaccgtgaa aggtgatgta cacgacatcg gcaaaaacat
     6721 cgttagcgtg gtgttgcagt gcaataactt tgaagtgata gacttaggtg tgatggtgcc
     6781 tgccgatgta attatcgaaa ctgctattcg ggagaaagcc gatctcattg ggttaagcgg
     6841 actaattacc ccatcgctgg acgaaatgga gtatttttta ggtgaaatga accgcttgaa
     6901 gctcgatatt ccggtgctga ttggcggcgc aacgacctca aaagagcata cggcgatcaa
     6961 actttatccg aaatataact accccgtagt ctataccgcc aacgcttcac gttgcgtaac
     7021 cgtctgtgcg accttgatga accctgaggc aaaagccgaa ttttgggaaa aaaccaaagc
     7081 cgaatacgaa gaaattcagc gtaaatttgc cgttcgcaaa ccacttcgca acggtttatc
     7141 tattgaggag gctcgagcca atcgttttga cggatttagt ggcgaatggg cagactatgt
     7201 gccgccaacg ccaaaacaga ccggcattat cgagtacaaa aatgtaccga ttgccaccct
     7261 acgcaaattt atcgactggt cgccgttctt tatgctctgg ggcttaatgg gcggttatcc
     7321 tgatgccttt gattatccgg aaggcggcga agaggcacgc cgtgtctata acgatgccca
     7381 acgggtgctg gacgaatttg agcaaaacgg caagctcaac ccaagtggga ttatgggcat
     7441 tttccctgcc gtgcgtgagg gcgatgatat tgcaatttta aacgaagatc gcaccgcttg
     7501 tatcggcaaa tcctaccacc tccgccaaca atccgaacga ggcaaaaaca gcaaaagccc
     7561 gtacaacctc tgtttaagcg attttatcgc cgaccgagaa agcggacaac aagactggtt
     7621 cggaatgttc gccgtttgtg ccggcattga agaacactat ttagttgaaa gctacaaagc
     7681 ggcaggcgat gactacaacg ccattttgct ccaagccgtt ggcgaccgct tggcagaagc
     7741 aatggcggaa tacctacact ttgagctacg caccaaagtc tggggctaca ccgtggaaga
     7801 cgaagacaac ggacgcttaa tccgtgaaga atacatcggc atccgccctg cgcccggcta
     7861 cccaagctgc ccggaacaca ccgagaaaca gataatttgg gacttgttag aggtggaaca
     7921 acgcatcggc atgaaactca ccgaaagcta tgccatgtgg ccggcagcaa gtgtctgcgg
     7981 ctggtacttc acccaccctg ccagcaacta cttcactctc ggcaagattg atgaagatca
     8041 ggtgttggat tacgccaaac gcaaaggctg ggacgaactc acggcgaaga aatggttaag
     8101 tgtggcgatg aaacaagatg tcaagtcgca ggctgaaaac atttaatgta gtggcgggtt
     8161 ttaacccgcc tgttacaagc ggttggtttt gagataaaat atgcaaaatt tatctctctt
     8221 ttatgcaaag aagttatcat cgagaacaaa acaatgattg aaaaactaaa ttatcactta
     8281 gtacaacttt taaaagacag taattatcaa ttaactcaat tttctcctgc gtatataaaa
     8341 aaattagagg aatctatttt cctcaaatca gggaaaaatg gagatattcc tcatattcaa
     8401 tgtcttgttc gtaaaaagga tattaagctc acacctgaag aagcagtacg ccaactctat
     8461 ttaatgaatt tgcattatga atatgattat ccatttgaac gaatggaatt agaatattct
     8521 gttgtattcg ggcgtgagaa gaaacgagcg gatattgtca tctttgatag agataatcct
     8581 aacgctgtct atattatggt ggagctgaaa aagccgaaat taaaagatgg taaagaacaa
     8641 ttaaaatctt attgtaatgc gactggagca cctattggtg tatggacaaa tggcgaaagt
     8701 atttcttact ataatcgccg tgatcctaat tattttaaag atattcccga tattccgaat
     8761 gctcaacaaa aactttctga tttattacaa gaacgttgga ctattcaaga tctgatagat
     8821 aatgacaaaa ttgccaaaca acgtaaatca ttgaaagatc ttgttattga aatggaagat
     8881 gaggtactgg cgaatgctgg agttgatgtt tttgaagagt tatttcaact tatttttact
     8941 aaactctatg atgaaatgga aagtacacgc aataaaagtc gttatttaga gtttcgtaac
     9001 tatggtgata ccgaaagtga attaaaggca aaaattcaat ctctatttga tcgtgcaaaa
     9061 aataaatggg aaggcgtatt tgctgaaagt gccaaaatca acctttcccc ttcgcatttg
     9121 gcgatttgtg tatcttcatt ggaagaagta aagctgttta attctaacct tgatgtagtg
     9181 gatgaagcat ttgagtactt aattaataaa tccagtaaag gagagaaagg acaatacttt
     9241 acgcctcgtt acgtcattga tatgtgcgta aaaatgctcg atccaaaacc tgaagaaaca
     9301 gtcattgata ccgcagcagg aagctgtggt tttccagttc attctatttt tcacgtttgg
     9361 gaaaaacaac ttaaagcgag aggattagaa cgctcacact tatttactgc tgaagaaaaa
     9421 ttgcctgaac aaacagatta tgtgaaagaa aaagtctttg cgattgattt cgatgaaaag
     9481 gcagtacgtg ttgctcgtac cctaaactta atcgcaggag atgggcaaac caacgtacta
     9541 catttaaata ccttagatta tgaacgttgg gaagatttta ctaaggaaga agaatggaat
     9601 gatgtatatg gagaaggctg gaaaaaatta agaaaattac gtaaaactaa aaatgaaaat
     9661 cgagatttcc aatttgatgt gctaatggca aatccgcctt ttgccggtga tattaaagaa
     9721 actcgtatat tagctcgcta tgaattaggc aaaaatagca aaggaaaaca gcaatccaaa
     9781 gtcggacgag atattttatt tattgaacgc aatttagatt tcttaaaaga cggtggacga
     9841 atggcgattg tgctaccaca agggcgtttt aacaattcca gcgataaata tattcgtgat
     9901 tttattgcag agcgttgccg cattttagct gttgtgggct tacacggaaa tgtgtttaaa
     9961 ccacataccg gcacaaaaac ttccgtacta tttgtgcaaa aatggcacga tgaactttgc
    10021 ccgaaagtag aggattaccc gattttcttt gccacaatgc agaaatccag caaagataat
    10081 tcaggcgata aaatctatgt taaaaacgct gatggcacac tcaaattaga caaccatcaa
    10141 cacctaattg tggagcacga tctatttaat cacgataata gtacccaaga cggcattgca
    10201 gaagccttta ttgaatttgc taaaaaagaa aagctctctt ttgtgggaaa ggattaagcc
    10261 catttgaccc tgctaaatat caggcattgt tagatgggct agaggtgagc gaggt
//
LOCUS       DANJWC010000081         9948 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.081, whole genome shotgun sequence.
ACCESSION   DANJWC010000081 DANJWC010000000
VERSION     DANJWC010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9948
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.081"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..1198)
                     /locus_tag="PWX02_001459"
     CDS             complement(<1..1198)
                     /locus_tag="PWX02_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HDL1729463.1"
                     /translation="MERITQRITQNLETLKQFTATPGNGCTRLPFSPEARSAVNYLRD
                     LIVEVGLRVKEDEAGNIFGILEGEEPNLPCVMSGSHYDSVLHGGDYDGIAGVITALET
                     ARLLKESGKKFKRSFVVAGFNDEEGMRFGTGYFGSGAMLGLRDAEYCKKFLDKDGISI
                     YEAMRNYGLDPEKVQNAKWEEGSIGHFIETHIEQGPVLENQGLEIGLVTGIVGLHRHM
                     FTVHGRADHAGTTPMDMRLDAIDVATKVIAKIADWAREKNDGTVATVGYMNVLPGGIN
                     IVAEKVIFSVDIRSLNMANIEDITAKIDAALAEETKKIGSRFDKETKLVIQPVKLSEK
                     MLNILEEATKARQFSYKRLPSGAGHDSLEIGQKIPTVMIFVPSKEGRSHTPVEFTEYH
                     YFSKAAV"
     gene            complement(1239..1454)
                     /locus_tag="PWX02_001460"
     CDS             complement(1239..1454)
                     /locus_tag="PWX02_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729464.1"
                     /translation="MTVKSTLESSFFKTKAGKMTLAFIVIMLAFALIMLGIYQVNNVF
                     IHIGFVLMLVAMLYSPVDVFILNRKKN"
     gene            complement(1464..3068)
                     /locus_tag="PWX02_001461"
     CDS             complement(1464..3068)
                     /locus_tag="PWX02_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDL1729465.1"
                     /translation="MDIPVNFFMWFMALLPIITLVILMVKFQWGATEAAPVGLLITII
                     SGIFLYKADVTLIAIESAKGIWYALIILLIVWTAVLMYQVSDAAKAFLVIRSEMSKLF
                     PNELLLVLAMGWIFESFLQGITGFGVPVAVGAPLLIGIGVRPLWAVVLPLLGQAWGNT
                     FGTLAAAWDALAGSAGLEIGNEKYFLTALWSSAFLWFWIVITGLAIAWFYGRGKALKK
                     GLVATLIIATIQGGGELLLSQVNTTISAFIPACLSILAIVLISKMSMYKEVWRVEESP
                     IMDRTKTTETEEALPNMNLLQAFIPYIVLSVMTLAILVITPVNQFLSQFKFGFAFPET
                     VTGYGFLNKAYTSYSPIAIFTHASMFLFISSIIGLIYYQRKGWIAKGGTRAIFAKSIS
                     MTMPSGIAVVGLVIMSKIMGGTGQTAVLAEGMAAVLGKTYVILSPFIGLLGTFMTGSN
                     MSSNILFGDFQMTTSKLLNANSAAVLGAQTAGGAIGSAISPSKIILGTTTANILGKEG
                     DVLKTIIWITLLPILLIGVVAYFASL"
     gene            complement(3129..4061)
                     /gene="arcC"
                     /locus_tag="PWX02_001462"
     CDS             complement(3129..4061)
                     /gene="arcC"
                     /locus_tag="PWX02_001462"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDL1729466.1"
                     /translation="MSKQTIVLALGGNALGNNLVEQREAVAKTAKVIVDILQQGKNIV
                     ITHGNGPQVGMIQRAMDDFAKSSGDEIPLPTSVAMSQGYIGIDLQNAIKYELVTRGIE
                     QKVSTILSQIEVDPKDAAFQNPDKPIGRFLTKEEADELEAKGIRTMEDAGRGYRIVVP
                     SPKPKRICELETIKTLVEAGHIVITCGGGGIPVVADENGRLVGVNAVIDKDNASSLLA
                     EKLNADYLVILTAVEKVAINWGKPNQEWLSELSTEQARQYIAEEQFAKGSMLPKVEAA
                     LKFAESGEGRRALITLLDKAAEGIAGETGTVIYS"
     gene            complement(4196..7195)
                     /gene="fdrA"
                     /locus_tag="PWX02_001463"
     CDS             complement(4196..7195)
                     /gene="fdrA"
                     /locus_tag="PWX02_001463"
                     /inference="COORDINATES: protein
                     motif:HMM:NF004760.0,HMM:NF018275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDL1729467.1"
                     /translation="MLKTVVKKGSYQDSVVLMLLTNAISGLEGVNKVSVMMATPANKD
                     IFAQSGLDTAELLEATANDMVVVADVDQEDVINVVMEKVEAFLKQKSQASSSHSGTEV
                     VKSWNKATASLPGANLAVISIPGAYAALEANRALDEGLNVFMFSDNVSVEDEKALKEK
                     AHRKGLVVMGPDCGTGIIQGVPIAFTNSVTPGSIGIIGASGTGIQELTTIIDRLGEGV
                     ENAIGTGGRDLYEDIGGITMLDAIEAMEQNDKVKVLIVISKPPAKAVREKISARLSRY
                     SKPVITLFLGEKPTFHEENFYHAYTLDEAARLAVALVRQEPIPTFAKNSTNSTACGKT
                     LKAYYSGGTLAGEAAMLLKDALRIEGSGSKADGFMFKQDGHIVVDLGDDIYTQGKPHP
                     MIDPAKRIESMRDAVDDATTGVILFDIVLGYGSHEDMATALIPTIQELQQKAKAQSRD
                     VAFVATVCGTRSDYQGYDETVRKLVEAGVEVCETNKAAVEKSLDLLGLPFDEPTKPIQ
                     PKTVVQGENSPASEQLLRLLSEKPKIINIGLKSFADVAEKFGCQVVQFNWQPPAGGNI
                     QLIKALNFLNEYEAVDIDEANRKVIAKVVAATPIIRDNVLAKTVIKELNDGKVILHAG
                     PPIQYKDMPNTVQGSCVGAVLFEKWATDEASARALLENGDITFMPCHHVNAVGPMGGI
                     TTANMPVWVVENATDGNFAYCTMNEGIGKVLRFGAYSDEVVKRLEWMRDVLGPTLGKA
                     IRSMENGLAVNPLVAKAIAMGDEFHQRNIAASMSFFKEVAPRITAMSDLAENDKYDVI
                     KFLADTDQFFLNIMMASCKAIMDGARTITEGTVVTAMCRNGVHFGIRIAGMGDEWFVG
                     PVNTPQGLYFTGYDGEDACPDIGDSAITETLGVGGLAMIAAPAVTRFVGAGGYEDALR
                     TSNDMMEICIDRNPNYIVPNWNFQGACLGVDARLVVEKGITPVINTGIAHKVAGFGQI
                     GAGTVRPPLACFEKAVLAYARKLGFNE"
     gene            complement(7277..8329)
                     /gene="allD"
                     /locus_tag="PWX02_001464"
     CDS             complement(7277..8329)
                     /gene="allD"
                     /locus_tag="PWX02_001464"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HDL1729468.1"
                     /translation="MKLSREELKGLMKAKLMKAGLNDQHADITAEILTWSDERGYHSH
                     GAVRMEYYCERIFKGGITHNPKFEWKETGPASAIFEGDNGCGYVAATFAMEKAIEMAK
                     KSGIAIVGIRNISHSGAIGYYTEMAAKEGLLSISFCQSDPMAVPYGGSEPYYGTNPIS
                     FAAPADEGRNVVFDMATTVQAWGKILDKRSRGESIPPDWAVDVDGNPVTDPNKVNALV
                     PISGAKGYGLMMMVDIFSGILLGVPFGKHVSSMYHDLSEGRRLGQMHIVIDPACFCGA
                     EQFRENMAQSINELNEMSPAPGNDKVYYPGQRAEMRRDKYAATGGIEIVDKVYEYLIS
                     DDIHFDRYDHKNRFAD"
     gene            complement(8351..9136)
                     /locus_tag="PWX02_001465"
     CDS             complement(8351..9136)
                     /locus_tag="PWX02_001465"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HDL1729469.1"
                     /translation="MGYLNGQTGYRQGLLETRSVIRKHNFVILETDGLVRNSIPNYHN
                     CDISILSSPALGASFVDYIAVVHPNGGCTQLGGNGIEVFVYLISGQLAVKNGDTDVVL
                     ESGGYIFSPADQVLSFINKSEHATKIYIHRRRYTALEGHQAITYVGNVNEIDYVEYEG
                     MKTCLIKDLLPSATDFGFDMNMHILLFKPGASHGYIETHFQEHGMLFLSGKGMYRLDD
                     EWIPVKKDDYVFMDSYCPQACYAVGDEDFVYIYSKECNRDVEL"
     gene            9210..>9948
                     /locus_tag="PWX02_001466"
     CDS             9210..>9948
                     /locus_tag="PWX02_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL1729470.1"
                     /translation="MLKATQITLNTVQVQDTALVIEATHYLDDNIAPKANLIYLHGGG
                     LLYGTKEDLPQRHLETFTQAGLQIFAINYPLAPQAQIEEIISSIQQSIKHLQNQLHLE
                     LPYFLFGRSAGAYLALLTAVRLSQQIEIEKPKGVISYYGYGFLKDGWFDSPSPHYLKL
                     PLVIENTVQSLIATPSQEADLNTHFSCYVHARQTGKWKSLIYQGRDKFFYLDYSLRLA
                     ELNLPIFAAHATNDPDVPFAEFQALCEK"
BASE COUNT         2930 a         2372 c         1667 g         2979 t
ORIGIN      
        1 ataccgctgc ttttgagaaa tagtgatact cagtaaactc tactggagta tggctacgac
       61 cctctttact tggaacaaaa atcatcacag ttggaatttt ttgcccaatt tctaatgaat
      121 cgtgcccagc tccacttggc aagcgtttat agctgaattg acgagcttta gtagcctctt
      181 ctaaaatgtt taacattttt tcagatagct taacgggttg aataaccaat tttgtctctt
      241 tatcaaatct cgaaccaatt tttttagttt cctccgctaa tgctgcatca atttttgccg
      301 taatatcttc aatatttgcc atatttaaag aacgaatatc aacactaaag atcacttttt
      361 ctgccacaat gttaatccca ccaggtagaa cattcatata gccaaccgtc gctacagtgc
      421 catcattttt ctctcttgcc cagtctgcaa ttttagcaat cactttagtt gccacatcaa
      481 tggcatctaa acgcatatcc attggggttg tgcctgcatg atctgctcgc ccatgaaccg
      541 taaacatatg acgatgtaag ccaacaatac ccgttactaa accaatttca agcccttgat
      601 tttctaacac cggtccttgc tcaatatggg tttcaataaa gtggccgatt gaaccctctt
      661 cccattttgc attttgaact ttttctggat ctaaaccata attacgcatt gcttcataaa
      721 tggaaatgcc atctttatct aagaattttt tgcaatactc tgcatcacga agccctaaca
      781 ttgcacccga tccaaaataa cctgtgccaa aacgcatacc ttcttcgtca ttaaagcctg
      841 caacgacaaa gctacgttta aatttttttc cgctttcttt caataaacga gccgtttcca
      901 gtgccgtaat cacgcctgcg ataccatcat aatcgccacc atgaagcaca ctgtcatagt
      961 gagaaccaga catcacacag ggtaaattgg gctcttcgcc ttctaaaata ccgaaaatat
     1021 ttccagcttc atcttctttc acacgaagac caacttccac gattaaatct cttaaataat
     1081 ttaccgcact tctcgcctct ggtgagaatg gtaaacgtgt acaaccatta cctggtgtcg
     1141 cagtaaattg ttttaaagtt tctaaatttt gcgtaatacg ctgagtaatt cgttccattt
     1201 tttattcctt tttataaacg aaaatatttc gccatacgtt aatttttctt acgatttaga
     1261 ataaatacat caactggcga atataacatt gccaccaaca ttagcacaaa tcctatatga
     1321 ataaagacat tattcacttg ataaatgcca agcataatca atgcaaacgc taacataatc
     1381 acaatgaaag caagagtcat tttgcctgct ttagttttaa aaaaactact ttctaatgta
     1441 gattttactg tcataaactc ctcctaaagt gaagcaaaat aagccacaac gccaattaat
     1501 aaaataggaa gtaacgttat ccaaataatt gtttttaaca catcgccttc tttacctaaa
     1561 atattagccg ttgtcgtacc taaaattatc ttacttgggc taattgcact gccaatagcg
     1621 ccgcctgcag tttgggcacc aagcacagca gctgaattcg cattcaggag tttagaggtc
     1681 gtcatttgaa agtcaccaaa taaaatatta gaactcatat tactacccgt cataaaagta
     1741 cctaacaaac caataaatgg tgataaaatc acatacgttt tacctaatac cgcagccatt
     1801 ccttcagcca acacggcggt ttgccctgtc ccccccataa ttttcgacat aatcactaag
     1861 cctactaccg caatgcctga tggcattgtc atcgagatag attttgcaaa aattgctctt
     1921 gttcctcctt tggctatcca gcccttacgt tggtaataaa tcaagccaat aattgaagag
     1981 ataaataaga acatacttgc gtgagtaaaa atggcaatag gcgaataaga ggtataagcc
     2041 ttattcaaaa agccatagcc tgttactgtt tcagggaatg caaagccaaa tttaaattgt
     2101 gataagaact gattcactgg cgtaataaca agaattgcca atgtcattac tgatagcaca
     2161 atatacggaa taaaggcttg cagtaaattc atatttggca aggcttcttc cgtttcggtg
     2221 gttttagttc tatccataat tggactctct tccacacgcc aaacctcttt atacatactc
     2281 attttgctga taagcacaat cgctaaaatt gacaaacaag caggaataaa cgctgaaatt
     2341 gttgtattta cctggctaag taacaattcg ccaccacctt gaatagtggc aataattaag
     2401 gtggctacta agcctttttt cagcgctttg cctctaccat aaaaccacgc aatcgctaag
     2461 cctgtaatga caatccaaaa ccataagaat gcagaagacc aaagtgctgt taaaaagtat
     2521 ttttcattac caatttcaag cccagctgag cctgctaaag catcccaagc agccgctaat
     2581 gtaccgaaag tattacccca agcctgaccg agcaatggca atactaccgc ccataatgga
     2641 cgaacaccaa tcccaattaa tagtggagca cccaccgcaa caggcacacc aaaacctgtg
     2701 ataccttgta agaaactttc aaaaatccag cccattgcca ataccagtaa taattcgttt
     2761 gggaataatt tactcatttc cgaacgaatg actaaaaagg cttttgcagc gtcactgact
     2821 tgatacatta ataccgcagt ccaaacaatt aacaaaataa ttaatgcata ccaaatccct
     2881 tttgctgatt caattgcaat taatgtaaca tctgctttgt ataaaaaaat gcctgaaata
     2941 atggtaatta ataatccaac aggagcagct tctgttgctc cccattggaa tttaaccatt
     3001 aaaataacca atgtgataat cggcaataac gccataaacc acataaaaaa attcactgga
     3061 atatccattt tgtttaaaac aacctaatta caaaaaattg aaaaaaatga ccgcttgtgc
     3121 tagatatttt acgaataaat taccgtacct gtttcgcctg caataccttc tgccgcttta
     3181 tctaacaatg tgattaatgc tctacgccct tcgccacttt ctgcgaattt caatgcagct
     3241 tccaccttcg gcagcattga gcctttcgca aattgctctt cggcaatata ttggcgagcc
     3301 tgttcggtgc ttaattcaga aagccactct tggttaggct taccccagtt aatggcaacc
     3361 ttttctactg ctgttaagat aactaaatag tcggcattaa gtttttcagc caatagcgag
     3421 ctggcgttgt ctttatcaat aacagcattt acacccacta atcgaccatt ttcatcagca
     3481 accacaggaa tacctccacc gccacaagta attacaatat gccctgcttc cactaatgtt
     3541 ttaatagttt ccagctcaca gatacgtttt ggttttggcg aaggcaccac aatacgatag
     3601 ccacgcccag catcttccat tgtgcgaata ccttttgctt ctagctcgtc agcttcttct
     3661 ttagttaaaa atctgcctat tggtttgtct ggattttgga atgcagcatc ttttggatca
     3721 acctcaattt gcgacaaaat tgtactgact ttctgctcaa taccacgagt aaccaattca
     3781 tacttaatcg cattttgtaa atcaatgcca atgtaacctt gactcattgc aacactggtg
     3841 ggtaatggaa tttcatcgcc tgaactcttg gcgaaatcat ccattgctcg ctggatcatc
     3901 cccacttgcg gtccattacc gtgggtaatc acaatatttt taccctgttg taagatatcc
     3961 acaatcactt tcgctgtttt tgcaacggct tcacgctgct ctactaaatt attgcctaat
     4021 gcattaccgc ctaaggctaa tacgatggtt tgttttgaca ttatttctcc tttttatccc
     4081 tactacccca tacgagccgt atggggttct attatttcaa ccacgccgtg gctcataaag
     4141 ccctaggact tggtttattc aacctagcac gccttggcta ggttaatgca ggattttact
     4201 cgttaaaccc taactttctc gcatacgcaa gcacagcttt ttcaaaacaa gctagtggtg
     4261 ggcgtactgt gcctgcacca atttgaccga aaccagccac tttgtgggca atacctgtgt
     4321 tgattacagg ggtaatgcct ttttcaacca ctaatcttgc atcaacacct aagcaagccc
     4381 cttggaagtt ccagttcggc acaatgtagt tagggttacg gtcgatacaa atttccatca
     4441 tatcgttact ggtgcgaaga gcatcttcat aaccgccagc ccccacaaaa cgagttaccg
     4501 ctggtgcggc gatcatagcc aaaccaccca cacctaaggt ttcagtaatg gctgagtcgc
     4561 caatgtcagg gcaagcatct tcgccgtcat agccagtaaa atacaagcct tgtggtgtat
     4621 tcacagggcc aacaaaccat tcatcgccca tacccgcaat acgaatacca aaatgcaccc
     4681 cattgcgaca cattgcagtc acaaccgtac cctctgtaat ggttctcgcc ccgtccataa
     4741 tcgctttaca gcttgccatc ataatgttta agaagaattg atcggtatcc gctaaaaatt
     4801 taatcacatc gtatttatca ttttctgcca aatcactcat tgcagtaatg cgaggagcaa
     4861 cttctttgaa gaaagacatt gaggctgcaa tatttctttg gtggaactca tcccccatcg
     4921 caatcgcttt cgccaccagt ggattgactg ccaaaccgtt ttccattgaa cggattgcct
     4981 tgccaagtgt tggacctaat acatcacgca tccattctaa acgtttcacc acttcatctg
     5041 aatacgcacc aaaacgcaat accttaccga taccttcatt cattgtacag taagcgaaat
     5101 tgccatctgt agcattttct actacccaaa caggcatatt tgctgtcgtg attccgccca
     5161 tcggacccac cgcattcaca tggtggcacg gcataaatgt aatgtcgccg ttttctaata
     5221 aagcacgagc cgaggcttca tctgtcgccc atttttcaaa taatactgcc ccaacgcaag
     5281 aaccttgcac cgtgttcggc atatctttgt attgaattgg tggaccagcg tgtaagatca
     5341 ctttgccatc gtttagctct ttgatcaccg tttttgccaa cacattatca cgaataattg
     5401 gtgtggctgc cacaacctta gcaatcactt tacgatttgc ctcatcaata tccaccgctt
     5461 catattcgtt taagaaattc agtgctttga tcaactgaat gttaccacct gctggaggtt
     5521 gccagttaaa ttgcactact tggcagccaa atttttctgc tacatctgca aaacttttca
     5581 aaccaatatt aatgatttta ggtttttcag ataataagcg taataactgc tcagaagctg
     5641 ggctattttc gccttgcact acggttttcg gctggattgg tttggttggc tcatcaaatg
     5701 gtaaacctaa taaatctaat gatttttcta ctgcagcttt attggtttcg catacttcaa
     5761 cgcctgcttc taccaattta cgcacggttt catcatagcc ttggtaatcg ctgcgagtac
     5821 cgcatacggt tgccacaaaa gcaacatcac ggctttgagc tttagctttt tgttgcagtt
     5881 cttgaatggt cggaattaag gctgttgcca tatcttcgtg cgagccgtat cctaacacaa
     5941 tgtcgaataa aatcacgcca gtagtcgcat catcaaccgc atcacgcata gattcaatac
     6001 gcttggcggg gtcaatcatc gggtgcggtt tgccttgagt ataaatatca tcgcctaaat
     6061 ccaccacgat gtgtccatct tgtttgaaca taaacccatc ggctttcgag ccgctgcctt
     6121 caatgcgtaa cgcatctttc agcagcattg ccgcctcgcc agccaacgta ccgcctgaat
     6181 agtaggcttt taaggtttta ccgcaagcgg tcgaatttgt cgaatttttt gcaaatgttg
     6241 gaattggctc ttgacgcact aatgccaccg ctaaacgagc cgcttcgtct agggtgtaag
     6301 cgtgatagaa gttttcttca tggaatgttg gtttttcgcc caagaataag gtaataacag
     6361 gcttgctata acggcttaag cgagcagaga ttttctcacg taccgctttt gctggtggtt
     6421 tagagatcac gatcagcact tttactttgt cgttttgttc catcgcttca atagcatcta
     6481 acattgtgat accaccgatg tcttcgtata aatcacgccc ccccgtacca atcgcatttt
     6541 ccacgccttc gcctaagcgg tcgataatcg tggttaattc ttggataccc gtaccagacg
     6601 caccaataat accgattgaa cctggcgtta cgctgttagt aaatgcaatc ggcacacctt
     6661 ggataatccc tgttccacaa tctggcccca ttaccactaa acctttacga tgtgcttttt
     6721 cttttaacgc tttctcatct tccacactta cgttatcgct gaacataaaa acgtttaatc
     6781 cctcatccaa cgcacggttc gcttccaatg cagcataagc acctggaatt gaaatcaccg
     6841 ccaagtttgc acctggcaaa gaagcggtcg ctttattcca agattttacc acttccgtac
     6901 cgctgtgaga cgagcttgct tgagatttct gttttaaaaa tgcttctact ttttccatta
     6961 ccacattgat cacatcttct tgatcaacat ctgcgactac caccatatcg ttggcggtcg
     7021 cttctaataa ttccgcggtg tctaagccac tttgagcaaa aatatctttg tttgctgggg
     7081 ttgccatcat tacagatact ttatttaccc cttctaagcc tgaaatggca ttagtgagca
     7141 acattaacac taccgagtct tggtagctgc ctttttttac tactgttttt aacatattat
     7201 tcttcctaat tttataagcc tatacaagct gtatagggtt ctttgaattc agccacacta
     7261 tggctatatg ataagattag tccgcaaaac ggtttttatg atcgtagcgg tcaaaatgaa
     7321 tatcgtcact gattaagtat tcatagactt tatccacaat ttcaatacca cctgttgcag
     7381 cgtatttatc acgacgcatt tcagcacgtt gccctgggta atacacctta tcgttacctg
     7441 gtgctggaga catttcattt agttcattaa ttgattgtgc catattttca cggaattgtt
     7501 ctgcaccaca gaaacaagca ggatcaatca caatatgcat ttgacctaaa cgacgacctt
     7561 ccgataaatc gtggtacatt gaagaaacgt gtttgccaaa tggtacccct aataaaatac
     7621 ctgagaaaat atctaccatc atcattaaac cataaccttt cgctccagaa attggtacta
     7681 gagcattcac tttgttcgga tcggttacag gattgccatc aacatcaact gcccaatctg
     7741 gtggaataga ttcgccacga gaacgcttat ctaaaatttt accccaagct tgaacggtcg
     7801 ttgccatatc aaacacgaca tttctgcctt catctgcagg tgctgcaaaa gaaattgggt
     7861 ttgtgccgta ataaggttca gaaccgccat aaggcaccgc cataggatct gattgacaaa
     7921 atgaaataga taacaagcct tctttcgctg ccatttctgt gtaatatcct atcgcaccac
     7981 tatgagaaat gttacggata cctacaattg caatgcccga ttttttcgcc atttcaatcg
     8041 ctttttccat cgcaaaggtt gcagcgacat aaccacagcc gttatcgcct tcaaaaatcg
     8101 ctgaggcagg ccctgtttct ttccattcaa atttaggatt atgtgtgatg ccacctttaa
     8161 aaatacgctc gcaataatac tccatacgaa cggcaccgtg agagtggtag ccacgttcat
     8221 cagaccacgt cagaatttct gctgtaatat ctgcgtgctg atcgtttaaa cccgctttca
     8281 ttaatttagc tttcattaag ccttttaatt cttctcttga tagtttcatt gtcaattcct
     8341 caacgttaaa ttaaagttct acatcacggt tacactcttt cgagtagata tagacaaaat
     8401 cctcatcacc taccgcataa caggcttgtg ggcagtagga atccataaaa acataatcat
     8461 cttttttcac aggaatccat tcatcatcta aacggtacat tcctttacct gaaagaaaca
     8521 gcattccgtg ttcttggaag tgcgtttcaa tataaccatg cgacgcacca ggcttaaata
     8581 acaaaatgtg catattcata tcaaagccaa aatcagttgc agaaggaagt aaatctttaa
     8641 ttaaacaggt tttcattcct tcatattcaa catagtcgat ttcattcaca ttacctacat
     8701 aagtgattgc ctggtgacct tctaacgcgg tataacggcg acggtgaata tagattttag
     8761 tagcgtgttc cgatttattg ataaaactca gcacttgatc ggcaggcgaa aagatataac
     8821 caccgctttc tagaaccaca tcagtatcac catttttaac agcgagctgc ccgctaatca
     8881 aataaacaaa aacctcaata ccgttaccac ctaattgtgt gcagccacca ttcgggtgaa
     8941 ctacagcaat ataatcaaca aaagatgcac cgagtgcagg agaggataaa atactaatat
     9001 cgcagttatg gtaatttgga atactatttc tcactaaacc atctgtttcc aaaatcacaa
     9061 aattatgctt acgaattacc gaacgtgttt caagtaatcc ttgtcgataa cctgtttgac
     9121 cattcaaata tcccataata ttctccttct ttttatataa gttaagttga attattttgt
     9181 actataatat acaaaaatag aggttcatta tgctaaaagc aacacaaatc acattaaata
     9241 cggttcaagt ccaagacaca gctcttgtaa ttgaagcaac ccattatcta gatgataata
     9301 tcgcacctaa agcgaatctt atttatctac acggcggcgg cctactttat ggtacaaaag
     9361 aggatttgcc tcaacgacat cttgaaacat ttactcaagc tggtttgcaa atttttgcaa
     9421 taaattaccc gcttgcacca caggctcaga ttgaggaaat tataagctcc attcaacaat
     9481 ctataaaaca tttacagaac cagctacacc tagaactccc ttatttttta ttcggacgtt
     9541 ctgctggagc ttatcttgca ttactgactg ctgtcagact tagccagcaa atagaaattg
     9601 aaaagccaaa aggtgttatc tcttattatg gttatggctt tttgaaagat ggttggtttg
     9661 atagcccaag cccacactat ttaaaattac cattagtcat tgaaaatacc gttcaatcac
     9721 taatagccac tccaagccaa gaagctgatt taaacacaca tttcagttgt tatgttcacg
     9781 ctcgccaaac tggcaagtgg aaatcattaa tttatcaggg cagagataaa tttttctacc
     9841 tcgattacag cttacgtttg gcggaattaa atctaccgat tttcgctgct cacgccacaa
     9901 acgaccctga tgtgccgttt gcagagtttc aagccctgtg cgaaaaat
//
LOCUS       DANJWC010000082         9941 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.082, whole genome shotgun sequence.
ACCESSION   DANJWC010000082 DANJWC010000000
VERSION     DANJWC010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9941
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.082"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            87..221
                     /locus_tag="PWX02_001467"
     CDS             87..221
                     /locus_tag="PWX02_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/trimethylamine N-oxide reductase
                     NapE/TorE"
                     /protein_id="HDL1729471.1"
                     /translation="MRSELLKTLLLSGLILPLLMAFAVGAYGFIVWMLQLFVIGLPGA
                     "
     gene            225..1388
                     /gene="torC"
                     /locus_tag="PWX02_001468"
     CDS             225..1388
                     /gene="torC"
                     /locus_tag="PWX02_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HDL1729472.1"
                     /translation="MLGSIKALFTRPATKIGLGVLVGIGFIAGAISWHQFNKAMDHTS
                     TEEFCVSCHSMQQPLEELKKTAHWSNKSGVTATCSSCHLPHDYTEKYARKVQASREVW
                     AELSGKYKEEGSFEKHRLEMAEREWARFAANGSKECKACHSYERMNFDKMSEAARKAM
                     KPAAEKDQSCMDCHKGIAHHLPKADENALKSKFDQFVNKSPSQGKSYYTKGNAELFLD
                     EGLSQTIGQLETAVPVAFVQAGKNADLVELTMWRKEKGFGRIWYHDFGKNITDAVLTK
                     EFMANQPKFEVLETKEDPITGLNWQKVKLQAWIGKAQLLDDVTPIWANAENQYKTQCS
                     TCHRQPDVAHFDSNSWIGLFNGMVGFTNMDKQTGKEVLRYLQMHASDSEEAKH"
     gene            1488..3954
                     /gene="torA"
                     /locus_tag="PWX02_001469"
                     /pseudo
     CDS             1488..3954
                     /gene="torA"
                     /locus_tag="PWX02_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248692.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            3954..4544
                     /gene="torD"
                     /locus_tag="PWX02_001470"
     CDS             3954..4544
                     /gene="torD"
                     /locus_tag="PWX02_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HDL1729473.1"
                     /translation="MTLAISREERQFIYGWFNAMLARELSDEQLNALQAGEFDDFFAY
                     LSELGFAEQVAKFQAEVTACRQLEFPRLELAADFAQLFLLEGNLSAIPYASAYLSGEA
                     LAKNLAEMDRLLGEFGLQVNRETKEPSDHLCVYLTLFDRLLAEKPLETQQQFLQNQLM
                     SWLPAWFAKAKTVKTNTGVYPALLRLFEQVLLKEQR"
     gene            4684..5373
                     /locus_tag="PWX02_001471"
     CDS             4684..5373
                     /locus_tag="PWX02_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL1729474.1"
                     /translation="MQNNKTTYLNWYDLCVLTLILFGEAIYTSTQQYFITTEMQGNFE
                     ENLTFSNEQNYWALLTQLALLLIAMGYLKWRNFDFSRWVIRPSWLAFFSGIGLFILLS
                     LAMDLYFETLYGVYNLVYPEPFWAVLREVNISTVLYAILNGFYEEIFFLGICLAVKPE
                     YLKWAVIYSLIIRVSFHTYQGMESALGLGIWLGLIIYLLYSRSKQKNLFPFWVAHSIA
                     DILGLSLLAYF"
     gene            5531..6283
                     /locus_tag="PWX02_001472"
     CDS             5531..6283
                     /locus_tag="PWX02_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HDL1729475.1"
                     /translation="MKSIKKLTLATLILVGLTACGSGGSGSSSNNAKSKPQVELKKTE
                     EDKKAEEAKKIAEAKGIGNKEYKDGLNELEEKNETGKNGEEIRTYGYLYNSHYSVVTA
                     KMKQTLQENGRQMEPTVEVKGLKTENLPTEGKATYKGEAFDSHGNNSNSVVGGELIYN
                     VDFSSRTGSGLVKNVQGGSIELAQGEIKNDSIIASAHQKYNDQAVGNGSYNIQFFGPN
                     AEEIGGKIELNGEGEGSMKQMLGIAGTREEQK"
     gene            6332..7726
                     /locus_tag="PWX02_001473"
     CDS             6332..7726
                     /locus_tag="PWX02_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL1729476.1"
                     /translation="MYKKHFLFLLVSPLVMAETVEFQQDPKEFDGQRQAVKFEQEIAK
                     KDLKLTAYLEEDNSLKGIEQHINQAILRQDWAKLERLLGDYAKQEQFDPLLLDYALAA
                     LAYAKKDYRSAAFYYQQLLKKQPDLTYPRFDLALILKEDYRYREAEQEFKQLKTDDLR
                     NAVNSALAQIEEEESWKPDFYLQYTQTNNVNNASSSPTVNVNGRIFIKESDSLPQSAK
                     GVRYGVGLSKMRNIGGNHFVGVDLGYNGVYYWNNHDYSEQSVSLTPKYSYRTATYRIG
                     VSPFIEQNWLGSSRYNYQFGSSINAVKLFERGLISGSFSHLQKRYSEQHIADRYNGYR
                     NQLTLGTQWQAVKNWRFFANLNASREITREKAQSSNKWLATIGVIYQTPSWASSVSVG
                     YGKRYFADKHYLFGYKRQDKEYQANVAVWNRSWHWHGIVPKLNFRYQKIDSNIADFYS
                     RSNKEVFMTLEKLF"
     gene            complement(7778..8398)
                     /gene="nudF"
                     /locus_tag="PWX02_001474"
     CDS             complement(7778..8398)
                     /gene="nudF"
                     /locus_tag="PWX02_001474"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HDL1729477.1"
                     /translation="MKLTQYQHNDLQILNRETVYKGHFELQKITFRHKLFSGEMSGEV
                     VRELLIKGEAAALIAYDPILDKVVLIEQVRIGAYDAGQKKSPWLLELVAGMVDKGNEE
                     PAEVAMREAKEEAGLEVTQVQHALSVWDSPGGQLERLHLFLGLIDNTKVKSGEVHGLL
                     EEENEDILVHVVSREQAYQWVEDGTIDNVIAVIGLQWLQLNYQKFN"
     gene            complement(8407..8826)
                     /gene="psiE"
                     /locus_tag="PWX02_001475"
     CDS             complement(8407..8826)
                     /gene="psiE"
                     /locus_tag="PWX02_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HDL1729478.1"
                     /translation="MPKKSLEEHHSSFGKIASETFQWIMNIALLVTGALLSYSLFAEA
                     YSLYELSFSDSEKFQIVEKIVIFFLYFEFLALIVKYFKSNYHFPLRYFLYIGITAMVR
                     LIIVDHSNALHTLLFALSILVMIIALYIVHTDRLHKS"
     gene            complement(8836..9558)
                     /locus_tag="PWX02_001476"
     CDS             complement(8836..9558)
                     /locus_tag="PWX02_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDL1729479.1"
                     /translation="MQNFANIEIAFIHRKAPLPSDFSYPEMPGGLTEKQVQKWQSRRV
                     AFFLLTELFKKHHLDLDLLADMQRSPSGRTFVDSEQIDFNISHSGEWIAVIFSYSFAK
                     LAVGIDIEHPQKIRRYHDLICHYGNAEEQNVLLANHSPILSNLAERFYLSWCLREAIL
                     KSQGVGIVKLSEVKHLPLEKQIFSAHCPKGKLHFVSELPFYLSYFYQQPQNMLLSEPL
                     LYHWQNGQFQPIKCQSLVYDVN"
     gene            complement(9564..>9941)
                     /locus_tag="PWX02_001477"
     CDS             complement(9564..>9941)
                     /locus_tag="PWX02_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDL1729480.1"
                     /translation="ALTAIFAKVGLQGVDSDFATFIRTVVIIALIAFLTYSGKWQPLD
                     SLSSKNWLFLILSGLATGLSWSAYFKALQMGNASQVAPIDKFSLVLVTLFAVIFLGER
                     PSGQDWLGIGLVAGGVLLLAFKR"
BASE COUNT         2991 a         2036 c         2278 g         2636 t
ORIGIN      
        1 tctctatatt tttgattgat atcaaggcaa attgtttctg aagataaaat aattaacact
       61 tattagttaa taaaaaagga gttgaaatgc gttcagaact attaaaaacg cttttgttat
      121 cggggctgat tttaccgtta ttgatggcat ttgccgtagg agcttacggc tttattgtgt
      181 ggatgttaca actgtttgtt attggtttac cgggagcgta gaaaatgtta ggttcaatca
      241 aagcattatt tactcggcct gcaaccaaaa tcgggttggg agtgttagtt ggtatcggtt
      301 ttattgccgg agcaattagc tggcatcaat ttaataaagc gatggatcat accagcactg
      361 aagagttctg tgtgagttgc cactcaatgc aacagccgtt agaggagctt aaaaaaaccg
      421 cccactggtc gaataaaagc ggggtaacgg caacctgttc aagctgccac ttaccgcacg
      481 attataccga aaaatatgcc cgcaaagtgc aggcaagccg tgaggtgtgg gcagaattat
      541 ccggcaaata taaagaagaa ggctcatttg aaaaacaccg cttagaaatg gcagagcgtg
      601 agtgggcaag atttgccgcc aatggttcaa aagagtgtaa ggcttgccac agctatgagc
      661 gaatgaattt tgataagatg tcggaagccg ctcgtaaggc aatgaaaccg gcagcggaga
      721 aagatcaaag ttgtatggat tgtcacaaag gcattgctca ccacttaccg aaagccgatg
      781 aaaatgcact gaaatcgaag tttgatcagt ttgtgaataa atccccaagc caaggcaaat
      841 cttactacac caaagggaat gcggaattat tcctagacga agggttaagc caaactatcg
      901 ggcaattaga aaccgccgtg ccggtggcat ttgtgcaagc aggcaaaaat gcggatttgg
      961 ttgagttgac aatgtggcgt aaagagaaag gtttcggtcg catttggtat cacgatttcg
     1021 ggaaaaacat taccgatgcg gttctaacca aagagtttat ggcaaatcag ccgaaatttg
     1081 aggtgttgga aaccaaagaa gatccgatta ccggcttgaa ttggcaaaaa gtgaaactac
     1141 aagcgtggat tggcaaagct caattattag acgatgttac cccaatctgg gcgaatgcag
     1201 aaaaccaata caaaacccaa tgtagcactt gccacagaca gcctgatgtc gcccacttcg
     1261 attccaacag ctggatcggc ttattcaacg gaatggtcgg ctttaccaat atggataaac
     1321 agaccggcaa agaggtgcta cgttatttac aaatgcacgc ctccgatagt gaagaagcga
     1381 agcattaata atattactcc cctctttact aaagagggga attaatcgag cgttgaataa
     1441 cattctctgt actagctcac gtttttacaa ataaagaagg acaagatatg caacaatctc
     1501 gccgtcaatt tttaaaaaat atgtctgtga tggctgccgc tgtcacaatg cacgaatttc
     1561 ctcgtgcctc gcaatgtgtt tgcgagtgaa gacctcagtc aatggaaagt ttccggctct
     1621 cactgggggg caatgcgtgc caaaattgag aacggacgag tggcagaaat caaaccgttc
     1681 gagtttgata aacacccgac cgaaatgctc aacggcatta aaggcttaat ttacagcgat
     1741 tcccgtatcc gctacccgat ggtgcgttta gattggctca aaaaccgcca taacagcaac
     1801 accgcacagc gtggcgataa ccgctttgtg cgggtaacgt gggacgaagc cctcgacctg
     1861 ttctaccaag agttagaacg ggtacaaaaa gagtacggcc cgtgggcgtt acacaccgcc
     1921 aatgtgggct ggcgttcaac cggtcaattc cacagttgcg gtaaccatat gatccgagcg
     1981 attgcgatgc acggtaacag tgtcggcacg gcaggcgact attctaccgg tgcagggcag
     2041 accattctgc cgtatgtgct gggttcaacc gaagtctatt cgcagggcac gtcttgggaa
     2101 atcatcttaa aagagagcga aaacatcatt ttctgggcaa gcgacccggt gaagaacctg
     2161 caagtgggtt ggaactgcga aacccacgaa gcctatgcct acttggaaaa actgaaagag
     2221 aaagtggcag caaaaggcgt gaatgtgatc tgtgttgatc cggtgaaaag caaaacccag
     2281 aatttcttag gttgcgaaca gcagtacatc aatccacaaa ccgatgtgcc gtttatgttg
     2341 gcacttgccc acacgctcta caccgagaac ctgtacgaca aaaaattctt ggatatgtac
     2401 acggtcggct ttgaaaaatt cctgccgtat ctgttaggcg aaaccgaaga caaagtggcg
     2461 aaaaccccgg aatgggcggc tgaaatttgt ggtatttccg ccgacaaaat ccgtgaattt
     2521 gcccgaatgc ttgccggcaa acgcacccaa tttattttcg gctgggcaat tcaacgccaa
     2581 caacacggcg aacaaccgta ctggatggga gcggtgctag cctcaatgtt agggcaaatc
     2641 ggcttggaag gcggcggcat cagctatgct caccattaca gctctatcgg tattcctgaa
     2701 tccggtgcgg caatgccggg ggcgttcccg ctcaatattg atgaggggca aaccccgaaa
     2761 tacgacaaca aagattacaa aggctacagc gacaccattc cggtggctcg tatgaccgac
     2821 agcttgctcc acgccggcga aaccattgat tacaacggca aaaaagtgac ttacgctccg
     2881 ttcaaaatgg cgattttcac cggctgtaac caatggcacc gccattccga gcggaacaaa
     2941 atgaaacagg cgttccaaaa actggaaacc attgtttcga tcaactacag ttggacggca
     3001 acctgccgct tctccgacat cgtgctgccg gcctgtaccc catttgaacg caacgatatt
     3061 gacgcctacg gctcatacag caaccgtggc gtgattgcaa tgcacaagct ggttgatcca
     3121 ctctacgact ctcgctccga ctttgagatt ttccgagatc tctgccgccg tttcggtaaa
     3181 gagaaagagt acagccgtgg aatggacgaa atgcagtggg tagaaaaact ctacaaagat
     3241 tgtcgccgtg aaaacagagg taaatttgaa atgccggagt ttgctgaatt ttggcaaaaa
     3301 ggctatgtgc tgttcccaga gggcaaacct tgggttcgcc acgccgattt ccgtgaagat
     3361 ccggaattgc acgcacttgg cacaccgtcc ggctttatcg aaatttacag caacaaaatc
     3421 gcctctttcg gctatgacga ttgcaaaggt cacccaatgt ggtttgagaa agccgaacgt
     3481 tcacacggcg gtaaaaattc cgacaaattc ccgtactggt tgcaatcagt tcacccggac
     3541 aaacgcttac actctcaact ttgtgaatcg aaagaactgc gtgaaactta cagcgtgcaa
     3601 gggcgtgaac cgctttacat taacccacaa gatgccgcca aacacggcat tcaagacgga
     3661 gatttagtgc gagtgtttaa cgaccgtggg caagcgattg tgggggcggt gctttccgac
     3721 aacttcccga ccggcgtagt gcgtttacaa gaaggggctt ggtattcacc ccttgacgaa
     3781 agcgtggggg cgattgacac ctatggcgac ccgaacacaa tgaccctcga catcggcacg
     3841 tccaagttgg cacaagcggt ttccgccaac acttgcttgg tgaacatcga gaaatttatc
     3901 ggcaatgccc cggctccgaa tggctttaac ggtgcgattg aggtaacggc ttaatgacgt
     3961 tggcaatcag tcgtgaagaa cggcaattta tctatggctg gtttaacgca atgctcgccc
     4021 gagaactttc ggacgagcag ttaaatgcct tacaagcggg cgaatttgac gatttttttg
     4081 catacctcag cgaattgggt tttgccgaac aggttgcaaa atttcaagca gaagtgaccg
     4141 cttgtaggca gctggaattt cctcgtctag aactggcggc tgactttgcc caactgtttt
     4201 tattggaagg caatctcagt gccattcctt acgcctccgc ctatttaagc ggcgaagcgt
     4261 tagcgaaaaa tttggcggaa atggacagac tgctgggcga atttgggctg caagttaatc
     4321 gagaaaccaa agagccgagc gatcaccttt gcgtttatct cacccttttt gaccgcttgc
     4381 ttgctgaaaa accgttggaa acgcaacagc aatttctgca aaaccaactg atgagctggc
     4441 tccctgcgtg gttcgccaaa gccaaaaccg tcaaaactaa caccggcgtt tatccggcgt
     4501 tgctaaggtt gtttgaacag gtgttgctga aggaacaaag ataaagttat ttatttgaga
     4561 atgatttgta ttgagcgaat tgagaaagga agttaaaatt tcaacttcct tttttattgg
     4621 ctggatttat ccacattatt acaagcggtt atttttataa aaaaatttac aaaggagaca
     4681 ttaatgcaaa ataataaaac cacctaccta aattggtacg atttatgcgt gctaaccctg
     4741 atcctgtttg gtgaggcaat ttatacctca acccagcaat atttcatcac caccgaaatg
     4801 caaggtaatt ttgaagaaaa tttaaccttt tcaaatgaac aaaattattg ggcattactg
     4861 acgcaacttg cattgttgct gattgcaatg ggctatctaa aatggcggaa ttttgatttc
     4921 agccgttggg tgattcgtcc ctcttggctg gcttttttta gcggtatcgg gttgtttatc
     4981 ttgctctcgt tagcgatgga tctctatttt gaaacattat atggtgttta caacctagtg
     5041 tatccggagc cattttgggc agtattgaga gaagtgaata tttctaccgt gctgtatgcg
     5101 attttaaacg gcttttatga agaaatcttt ttcctcggta tttgcttggc ggttaaacct
     5161 gaatatttaa aatgggcggt gatttattca ctgattattc gggtgagttt tcatacctat
     5221 caaggaatgg aaagtgcgtt aggattagga atttggctcg gtttaattat ttatttgctc
     5281 tactcacgct caaagcagaa aaacctattt ccattttggg tggcacacag tattgcggat
     5341 attttggggc tgagtttgtt ggcttatttt tagttaaagg tgctacattt aagcggcata
     5401 agttgataga gctgatcgta ccagtagcat aaaaacatta ttatttgtat ggaaaggtgc
     5461 ttttaggtta caatataacc gatagttatt ggttataact aaaaattatt tttaatttaa
     5521 cggagcaaat atgaagagta tcaaaaaatt aaccctagcc actttgattt tagtgggttt
     5581 aactgcttgt ggtagcggtg gttcaggtag cagttctaat aatgctaaat cgaaaccaca
     5641 agttgagtta aaaaaaactg aagaggataa aaaggctgaa gaagcaaaaa aaattgcaga
     5701 ggcaaaagga attggcaata aagaatataa agatggatta aatgagttag aagaaaaaaa
     5761 tgaaacaggt aaaaacggag aagagattcg aacctatggt tatctctata acagccatta
     5821 ttccgttgta actgccaaaa tgaaacaaac tttacaggaa aatggtcgtc aaatggaacc
     5881 tacggttgaa gtgaaagggc ttaaaactga gaatttaccg acagagggca aagcaacata
     5941 taaaggagaa gcctttgatt ctcacggtaa taacagcaat tcagtagtag gtggagaatt
     6001 aatctataat gttgatttct ctagtcgtac cggatcaggt ctagttaaaa atgtacaggg
     6061 cggttctatc gagttagcac aaggcgaaat aaaaaatgat agtattatag cttctgctca
     6121 ccaaaagtat aatgatcagg ctgttggaaa tggtagttac aatatccaat tctttggtcc
     6181 aaatgcagaa gaaattggag ggaaaattga gcttaatggt gagggcgaag gctcaatgaa
     6241 gcagatgtta ggtattgcag gaacccgaga agaacaaaaa taacatttaa tacccaatgc
     6301 cgtttaatct taaacggcat tcttttttac tatgtataaa aaacattttc tatttttact
     6361 tgtttcgcct ttagtaatgg cggaaacggt tgagtttcag caagatccca aagagtttga
     6421 tgggcagcga caagcggtta aatttgagca agaaattgca aaaaaggatt taaaattaac
     6481 cgcttacttg gaggaagata attcccttaa aggtattgaa cagcacatta accaagcgat
     6541 tcttagacaa gattgggcga agctggaaag gctgcttggc gattatgcaa agcaagagca
     6601 atttgatccg ctacttttag actatgcttt ggcggcattg gcttatgcta aaaaagatta
     6661 tcgctctgcg gcgttttatt atcagcagtt gctgaaaaaa cagcctgatt taacctaccc
     6721 tcgttttgat ttggctctga ttttaaaaga ggattatcgc tacagggaag cagagcaaga
     6781 atttaagcaa ctcaaaaccg atgatttacg caatgcggtg aacagtgcgt tggctcaaat
     6841 agaggaggaa gagagctgga agccggattt ttatttgcaa tatactcaaa ccaacaatgt
     6901 gaataatgct tcttcgtcgc caacggtaaa tgtaaacggg cggattttta ttaaagaatc
     6961 agacagcttg ccgcagtcgg caaaaggcgt aagatatggc gttggcttga gtaaaatgcg
     7021 aaatatcggc ggtaatcatt ttgtgggagt cgatcttggc tataacggcg tttattactg
     7081 gaataaccac gattatagcg aacagtcggt ctcactaaca ccaaaatata gctacagaac
     7141 ggcaacttat cgtatcgggg tttcgccctt tattgagcag aattggctgg gtagctcacg
     7201 gtataactat caatttggta gcagtatcaa tgctgttaaa ctttttgaac gagggctgat
     7261 ttccggctca ttcagccatc tgcaaaaacg ttatagtgaa cagcatattg ccgaccgtta
     7321 caatggttat cgcaaccaac tcacgctagg tacgcaatgg caagcggtca aaaattggcg
     7381 attttttgca aatcttaacg ctagccgtga aattacccgt gagaaagctc aatcttccaa
     7441 taagtggctg gcgaccattg gcgtaattta ccaaacccca agctgggcaa gttcggtaag
     7501 tgtcggatat ggcaagcgtt attttgctga taaacattat ctgtttggct ataaacgcca
     7561 agataaggaa tatcaagcga atgtggcggt gtggaatcga agttggcact ggcatgggat
     7621 tgtgccgaaa ttaaatttcc gctatcaaaa aatcgacagc aatattgccg atttctactc
     7681 ccgtagcaat aaagaagtct ttatgacgtt agagaaacta ttttaaggct gtgtatgaat
     7741 gtcggggaga aaaattttcg cccccatcaa tattaactta attgaatttt tggtaattta
     7801 actgcaacca ttgtagccca attaccgcaa ttacattatc aattgtgccg tcttccaccc
     7861 attgatatgc ctgttcacgg ctaaccacat gcactaaaat atcttcgttt tcttcttcca
     7921 ataagccgtg aacctcgccg gatttcactt tggtgttgtc aatcaagccg agaaacaggt
     7981 gcaggcgttc cagttgtcca ccggggctgt cccatacgct taaggcgtgt tgcacttgtg
     8041 ttacttccaa gccggcttct tctttggctt cacgcattgc aacttctgcc ggctcttcgt
     8101 tgcctttatc taccataccg gcaaccaatt ccagcaacca aggcgatttc ttctgccctg
     8161 catcgtaagc accgatacgc acttgctcaa ttagcactac cttgtctaag ataggatcgt
     8221 aagcaattaa agctgccgct tcgcctttaa ttaagagttc acgcaccact tcaccgctca
     8281 tttcaccgga aaacagtttg tggcggaaag tgattttttg tagctcaaaa tgccctttat
     8341 agacggtttc tcggtttaga atctgtaaat cattgtgctg atattgggtt agcttcatat
     8401 tagttcctag cttttatgta aacgatcggt gtgtacaata tataatgcta taatcatcac
     8461 caaaatagag agtgcgaata atagtgtatg caacgcatta gaatgatcga caataatcaa
     8521 tcttaccatg gcagtaatcc cgatatagag gaaatagcgt aacggaaagt gataattcga
     8581 tttgaaatat ttcacaatca acgcaagaaa ctcaaaatag aggaagaaaa tcacaatttt
     8641 ttccacaatt tggaattttt cactgtcgct aaaagagagt tcatagagtg aataggcttc
     8701 agcaaacagc gagtaagaaa gcaatgctcc tgttactagc aaggcaatat tcataatcca
     8761 ctgaaaggtt tcggaagcaa ttttgccaaa actggaatgg tgttcttcta aagatttttt
     8821 aggcattcgt tatccttaat taacatcata aacaagtgat tggcacttta taggctgaaa
     8881 ctgcccgttt tgccaatggt atagcaaagg ttcagataat agcatatttt gaggttgttg
     8941 atagaagtag cttaaataga acggcagttc gctcacaaaa tgcagcttgc ctttcggaca
     9001 atgggcggaa aagatctgtt tttctaacgg taaatgtttt acttcggaca gtttcacaat
     9061 ccccacccct tgcgatttca atatcgcctc tcgcaagcac cagctcaaat aaaagcgttc
     9121 ggcgaggttg cttagaatgg gggagtggtt ggctaataaa acattttgtt cttcagcgtt
     9181 tccgtaatgg cagattaaat cgtggtaacg tctgattttt tgtgggtgtt caatatcaat
     9241 gccgaccgct aattttgcaa aagaatacga aaaaatgacc gctatccatt cccctgaatg
     9301 gctgatgtta aagtcaattt gctcagaatc gacaaaagtg cgaccgcttg gggagcgttg
     9361 catatcagct aataaatcta aatccagatg gtgtttttta aacaattccg tcagcagaaa
     9421 aaaggcgact cggcggcttt gccatttttg cacctgtttt tcagttaaac cgccgggcat
     9481 ttcagggtag gagaaatcac tcggcagagg tgctttccga tgaataaacg caatttcaat
     9541 atttgcaaaa ttttgcataa aaatcaccgc ttgaaggcaa gtaacagtac gccaccagca
     9601 actaagccaa tgccaagcca atcttgtccg cttgggcgtt cgcctaagaa aattactgca
     9661 aaaagggtaa ctaacaccaa gctgaattta tcgattggag cgacttggga agcattaccc
     9721 atttgcaatg ctttgaaata cgccgaccaa gacaatcctg ttgccaagcc ggataagatt
     9781 aaaaacagcc agtttttgct gctcaagcta tctaacggct gccattttcc gctgtaagtg
     9841 agaaaggcaa taagtgcaat aataactacc gtgcgaataa aagtggcgaa atcagaatct
     9901 acgccttgta agccaacttt ggcaaaaatg gcggttaatg c
//
LOCUS       DANJWC010000083         9934 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.083, whole genome shotgun sequence.
ACCESSION   DANJWC010000083 DANJWC010000000
VERSION     DANJWC010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9934
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.083"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            140..1945
                     /locus_tag="PWX02_001478"
     CDS             140..1945
                     /locus_tag="PWX02_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HDL1729481.1"
                     /translation="MLSKLQRFTLMIKSTIIKHPLEILFVTYVTILLINNVSDYELIK
                     RFQNLIALFPICFILLYLIRDTKAYWLFALIPIAIVFFFDQINLTLLEDSRYWATIFC
                     VFLCLISQYWYKNNHYFVAGTTNKLVNIVFATVLSGIIFATLSAITIAIIALFDLNKQ
                     NNFHIFEKFGIFSSFWAFPVLFLTLEQQTTNDSRYLNRISEMLLNWILSPALIIYTAI
                     IYAYVIFIAAKGQMPDGVVANVALPYLAFGLAIQAVQLLLKNAKWQWFYSLFTYLALL
                     PLTLIWYAIYIRFSHYGLTEARIYLVIGTITLSICYTLLLFKQLAQYRLFSAVSIAII
                     LISVFILDPKTVALKDQTKRLDNYLLKHKLLDVNNKISTKAVLKYQESLNENRDEAEY
                     FYSMLHYVARGMPAAQFKEKYGVNSSYDLMSEIRERNTDPRYFEATNSELVRLTKADM
                     ANAREYITFSSQLYRPNSFSHQKIENKLCQKLLSQGYYFEGNILNIEYQYSETAQDCS
                     SITQDPTEFVIYFKGLGTTIEFNINDILKKIFAKHHLSITQQHKNEVLEKIKGDLLKM
                     DLGNSVLGLKHIELRFEDNIGYIVESISTQYIMLK"
     gene            complement(1988..5016)
                     /locus_tag="PWX02_001479"
                     /pseudo
     CDS             complement(1988..5016)
                     /locus_tag="PWX02_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4981 domain-containing protein"
     gene            5194..6051
                     /gene="menB"
                     /locus_tag="PWX02_001480"
     CDS             5194..6051
                     /gene="menB"
                     /locus_tag="PWX02_001480"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005723289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDL1729482.1"
                     /translation="MLYPSEEFLYAPVEWVDHSEGYTDIRYHKSKDGIAKITINRPEV
                     RNAFRPQTVKEMIEAFSDARFDEKIGVIVLTGEGEYAFCAGGDQKVRGDYGGYKDESG
                     VHHLNVLDFQRDIRTCPKPVVAMVAGYAIGGGHVLHMLCDLTIAADNAKFGQTGPKVG
                     SFDGGWGASYMARLVGQKKAREIWFLCRQYNAQEALDMGLINTVVPYADLEKETVRWC
                     REMLQNSPIALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNEKREP
                     DFSKFRRNP"
     gene            6134..8536
                     /gene="mrcB"
                     /locus_tag="PWX02_001481"
     CDS             6134..8536
                     /gene="mrcB"
                     /locus_tag="PWX02_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1B"
                     /protein_id="HDL1729483.1"
                     /translation="MSDVELNEQSEQEPEQKPKKRNYFLSTLFKIGVIGACSMAFYGI
                     YLDGKIRSKMDGQIWELPAEVYARIESIKIEDNLTLEQTKQALLDNGYRQVSQVATPG
                     DFKIEENQLVLLRRAFPFPETPESQRVLRLTFKQDKLTYIEDLVQGKLIEEFRLDPKL
                     LALLHSDSDEERQALRLQQYPRLLIESLILTEDRRFYQHDGISPIGIARALITNYQAG
                     RTVQGGSTLTQQLVKNLFLSNERSLVRKVNEALMSVILDFRYDKNRILETYLNEIYLG
                     QNGSYQIHGFALASQFYFGRPIQEISLDQIALLVGMVKGPSLYNPWRNPQGAVERRNV
                     VLRLLLDHQVINQELYALLSSRPLNIREKGTIYREQPAFMQALTIDLKNELGEIKSAQ
                     LSGTKIFTTLDRKQQRSAEQAVITGLENLESKNKKVSDLQSAIVVAEYRTGKVRAIVG
                     DRLTQFAGFNRAIQSKRQIGSLVKPSVYAIALAQPELFRLNTPIQNKQISIKVGSTYW
                     TPRNYDRSFSGSVMLMDALVRSLNIPTVNIGMKVGLKKVIAKQKEMGWDNAEIPPYPS
                     TLLGSYSISPYDVTRSYQVIANEGLKTPLTTIEAIISHNGEMFYQRDSGKVSEQVLPP
                     EAAVQTMYAMQQVVERGTARSLQNDFAKLKLAGKTGTTNNARDTWFVGIDGEHVTTVW
                     LGKDNNGDTNLTGSSGALYVYKQYLERALPQAYRLPKLNTMQWVGINSYGSWNCDSGR
                     QIPVWRDKGQRYCTSAGNVVATEKPSVWDALSLKKENIEAKAEEAEGKQPIPTEEVAP
                     IE"
     gene            8711..9217
                     /locus_tag="PWX02_001482"
     CDS             8711..9217
                     /locus_tag="PWX02_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDL1729484.1"
                     /translation="MAMSNIGLDKEIAKKLADELNGLLATYQVFYTNVRGYHWNVRGV
                     NFFELHAKFEEIYDDLVTKVDEIAERILTLGHTPQNAFSQYLTKSQIQEHIGVSAAQE
                     CLRGTLNGFKTLLGQQREILALASDANDEGTASQMSDYIKEQEKLVWMFTAACAECSD
                     YAQGCSCN"
     gene            9276..>9934
                     /locus_tag="PWX02_001483"
     CDS             9276..>9934
                     /locus_tag="PWX02_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HDL1729485.1"
                     /translation="MAMSQGFQFKQFFIRHDRCAMKVNTDGILLGAIAHIKDCQNILD
                     LGTGSGLVALMLAQRTDDCCHITALELEPNAYQQAVENAQNSAWANRISVLQGDVMQQ
                     AFEQKFDLIVSNPPYFADSLAARTTERDLARSISHYSHLNWLAKAKPWLAENGKITLI
                     LPIAAAEKLVDQSEQIGLYCVEQWLIFTKSGKLAKRSIVSFSLNKQKREHKELIIYDL
                     D"
BASE COUNT         2998 a         1917 c         2106 g         2913 t
ORIGIN      
        1 cctatagttg aataacttag gctttcttat tttaatgaat atgttcacct ctaaaaacaa
       61 ataaagatta aaactgttta cctattattt attcacatgt atactgtgag atattccttt
      121 cataggtagg agatctatta tgctaagtaa gctacaacgc tttaccttaa tgataaaaag
      181 caccatcatt aagcatccac tggaaatttt attcgttact tacgtcacga ttttacttat
      241 caataatgtg agcgactacg aattaatcaa acgctttcaa aatctaattg ctctttttcc
      301 tatttgcttt attttgcttt acctgatacg cgataccaaa gcttattggc tattcgctct
      361 gattccgata gcaatcgtct ttttctttga tcaaattaat ttaacattat tggaagattc
      421 tcgttattgg gctaccatat tttgtgtatt cctttgtttg atcagtcaat attggtacaa
      481 aaacaaccat tattttgtag ccggaacaac taataaatta gttaacattg tctttgctac
      541 agttttatct ggcattattt ttgcaacatt aagcgctatt accatagcca ttattgcttt
      601 atttgattta aataaacaaa acaactttca tatttttgaa aaatttggca ttttttctag
      661 tttttgggca ttccctgttt tatttttaac tcttgaacaa cagactacca atgattctag
      721 atatctaaac cgcattagtg aaatgttact aaactggatt ttgtctccgg cattaattat
      781 ctatactgca attatttatg cttatgtaat ttttattgcg gctaaggggc aaatgccaga
      841 tggggttgtt gcaaacgtag cgcttcccta tttagctttc ggcctagcta tacaagcggt
      901 gcaattatta ctaaaaaatg caaaatggca gtggttttat tctctcttta cctatttagc
      961 acttttgccg ttaacattaa tttggtatgc catctatatt cgtttcagcc attatggctt
     1021 aactgaagcc cgtatttatt tagtgattgg cacaataacc ttatctattt gctatacctt
     1081 attactattt aaacagttgg cgcaatatcg tctcttttct gcagtgtcga tagcaattat
     1141 tttaatttca gtgtttattt tagatccgaa aacagttgcg cttaaagatc aaaccaagcg
     1201 gttggacaac tatttgctaa aacacaaatt attagatgta aataacaaaa tcagcacaaa
     1261 agctgtactt aaataccaag aatcattaaa tgaaaaccga gatgaagccg aatattttta
     1321 ttcaatgctc cactatgttg ccagaggaat gcctgctgca caatttaaag agaaatatgg
     1381 cgtaaattca agctacgatt taatgagtga aataagagaa agaaatactg acccacgtta
     1441 ttttgaagct actaattctg agctggttcg cttaactaaa gcagatatgg caaatgctcg
     1501 tgaatatatt acatttagta gccaattata tcggcctaat tcattttccc atcaaaaaat
     1561 agaaaataag ctatgccaaa aactcctttc tcaaggttac tattttgagg gaaatatatt
     1621 aaatattgag tatcaatact cagaaacggc tcaagactgc tcttcaataa cacaagatcc
     1681 tactgaattt gttatctact ttaaaggact tgggactact attgaattta acattaacga
     1741 tattttgaaa aaaatctttg ctaaacatca tttatctatt acccagcaac acaaaaatga
     1801 ggttttagaa aagattaaag gcgatttact caaaatggat ttaggaaatt cagtacttgg
     1861 gcttaagcat attgagttaa gatttgaaga caatatcggt tatatagtag aaagtattag
     1921 tacacaatat attatgctga aataaaagga gatgctcgtt caattaaatt tgaacgagca
     1981 tttttcgtta aaaattcaat ttaagcagcc actcaaaatt agtctcattt aaccgatatt
     2041 gcggtttcaa attcgggccg caagagtttg agccaatgcc gctcattttg taatcgacac
     2101 acagcacggt ggaagggctt tctaccaaat cataactgtg gcttttagtg gttagctctt
     2161 cctgtgtgta aggcgaaaga ttaaaactaa acggctgtgg agaagtaaca gttatcccat
     2221 ttgcttgcac ataatgcgta ccgaagtggc tgccgttttc ctgcgggttg agataatcgg
     2281 tatgattttc tcgggtgctg gtttgataag ctccgagttt cgccaaatgg tatttgtcga
     2341 tataactctc ctgctcgcca tagccaaaat agctcacttg gttattccgt ttattcaaga
     2401 aaaaccgcaa gccaaagcga ggtaaaaacg gcaaatgagc ggggcgggtt gcctgaacct
     2461 gaatcgtcat ttcgccattc ggggcaagat gataattcac tttcagggtt aaaatccgac
     2521 tttgagcggt tgccacaatg ccacattcgg cagtaatgtt tacgccgttt ttatcttgca
     2581 tcagttcggt atgatacgcc ctggtgtaac agttatcgta gccggctttt ttccaaaact
     2641 cacgaattaa gcggtcgtta tcggttgggg ctcgccaaat attgaaatcc aacggttgct
     2701 caattactgc ctcgccgttt ttctcaatcc ggctgaaaat gcctttgagt ttatcgaaag
     2761 tgtagcgata gtcgccctgt tgaatccgcc attcatttgc aaaatcttgc acaaaaatga
     2821 ccgcttgcgg gcgtggatta agtgctggag tgaccatcgc ttgatgcagc gtgatttggt
     2881 caaagcctaa gtgatgccct ttttccaaca gagccgtgct ttcatttaaa gaataatcaa
     2941 gggttaaaag ttgcaatttg ccgtttggtt cgggcagcgt tagcggcaaa caagcggtct
     3001 gatttggctg acaatttacc aaacattgtc cctgcagggt ggtttcgccg ttttcgctga
     3061 aacagtaatg cacggtcaga taatcagcta agtcgctgaa atccaactga tttttcagcc
     3121 aaattttgcc cgcttttaac tcagctcgca ccgggcgatt cacatttttc acttctaaca
     3181 aattagagtg tggcacacgc tgttgggaaa ccaagccgtc catacagaaa ttgccatcgt
     3241 gcaaggtttc gccgaaatca ccgccatagc cgtatttttc ggtcttctgt tcggcactta
     3301 gctcagagtt ggggagcaga ggagcatggt cgcaccattc ccacacaaag ccaccgcacg
     3361 caccgctgta gcgttggaag gtttgccaat aatcttccat atcaccactc gaattgccca
     3421 tcgcgtgaga atattcacac aaaataaaag gtttgtggtt ggcagagtcg gaaaaatatt
     3481 cctctaaccg ccggtgcttg ggtacatctc gctgtataaa tcgaggtttg aaagatcgtt
     3541 ttgatgtgcc gaatgttggt aaatcgagcc ttcgtagtga gttaaacggc ttggatcccg
     3601 ttgttttacc caacgggctg cgacttcaaa attttcacca aagccggatt cgttaccaag
     3661 tgaccaaata ataaccgaag tgcggttttt gtctcgctcc acattggcaa tgttgcgatc
     3721 taaaatcgcc tgtttgaaca gcggatctct tgcaaaataa cagaaattat cgaccgcttg
     3781 ttggcgaatt tcttcctgtt tgttcgggtt tggcacattc aggaaaatgc tgttttccgg
     3841 actttcaata tacactgcac tggagccgtg agattcaaga tcactttcgg caatcagata
     3901 aaaaccgagc tgatcgcaca attcattaaa ccacggagcg ttcgggtaat gggcggtgcg
     3961 aatagcgttg atgttatggg ctttcattaa gcgtaaatcc accaacgctt gctcacggct
     4021 aatgctgtag ccggttttcg ggtcggaatc gtggcggttt acccctttaa atttaatcgg
     4081 ggcgttgttg aatagcatca cgccattttc cacacgaatc tgacgaaagc cgattttttg
     4141 ctcaataatt tcgttttggt agctcaagca tagcgtgtag agtgtcggat cttccgcatt
     4201 ccacaaacac aaattgcaaa tttcttggca aaaatcaccg cttgtttgct cgcagactaa
     4261 gcggttttca gggtcgaaaa gttgaaaatg cacagcttcg gtgtaggtgg aaaattgtgt
     4321 ttccaccttg agttctgcct ttgaaagatc agcgtttaac tggtagcgga taaagaaatc
     4381 ttgcaggtag ttttgctcac gttccaaaat ataaacatca cggaaaatgc ctgacattcg
     4441 gaatttgtct tgatcttcca aataactgcc gtcacaccat tttagcacca ccacgacgag
     4501 gctgtttttg ccctgtttaa gctgtttggt gatgtcaaat tcattggtgc tgtggctgat
     4561 ttgcccgtag ccgacaaatt cgccgtttac ataaacaaac aggcacgaat ctacgccctc
     4621 aaaatttagc aaatagcgtt tgttaggctt ggggttaaac gtgatttcac gctgatacac
     4681 cccgcaaggg ttttggtgtg gcacataagg cggatcaaac ggaatcgggt agttgatgtt
     4741 agtgtattga tgggcatcaa agccttgagt ttgccagtta gacggcacgg ggatttggtg
     4801 ggtaaatttg gtgttgagga aatcctccgg cagatcgtgc ggacttggat agaaattaaa
     4861 cttccaatcg ccatttagca aagtaaataa ctcagattgt tcccgttcca actgattcgg
     4921 cgtttgccct ttagcaaaag gaataaaata agcgtggtgt ggcgtggtat taaggtgcaa
     4981 cacactcggg tcttggtagt aattcggtaa cgacataaaa gccctatgat caaaaagata
     5041 taaatacgaa tacactttaa ttctcttata aaatagggca atcaactatg tgaaaaattg
     5101 tgaggcggat cacaaaataa tttcctcacc tttttccttc gattccgtta taatcacaag
     5161 aattttttat gtcatttttc aaggaattta ccaatgttat atccaagcga agaattttta
     5221 tatgcaccgg ttgagtgggt tgatcacagc gaaggttaca ccgatattcg ttaccataaa
     5281 tcaaaagacg gtatcgctaa aatcaccatt aatcgtccgg aagtgcgtaa tgctttccgt
     5341 ccgcaaacgg taaaagagat gattgaagcc ttttctgatg cccgttttga tgaaaaaatc
     5401 ggcgtcatcg tattaaccgg tgaaggcgaa tatgcgttct gtgccggcgg cgaccaaaaa
     5461 gtgcgtggcg attacggcgg ctacaaagat gaaagcggtg tgcatcactt aaatgtgttg
     5521 gatttccaac gtgatattcg tacctgcccg aaaccggtgg tggcaatggt tgccggctat
     5581 gcgattggtg gtggacacgt tttacatatg ttatgtgatt taaccattgc cgctgacaat
     5641 gcaaaattcg gtcaaaccgg cccgaaagtg gggtcatttg acggcggttg gggtgcaagc
     5701 tatatggcac gtttagtcgg tcagaaaaaa gcgcgtgaaa tttggttctt atgccgtcaa
     5761 tataatgcac aagaagcgtt agatatgggc ttaatcaata ccgttgtgcc ttatgctgat
     5821 ttagaaaaag agactgtgcg ttggtgtcgc gaaatgctac aaaacagccc gattgcctta
     5881 cgttgcttaa aagcagcatt gaatgcagat tgtgacggtc aggctggcct gcaagagctt
     5941 gctggtaatg caacaatgtt gttctatatg acagaagaag ggcaagaagg acgcaatgcg
     6001 ttcaacgaga aacgtgagcc ggacttcagc aaattccgcc gcaatccata attgacggca
     6061 aatacaagcg gttcgatttt gcaaaaaatt tgcaaaaatc gaccgcttac cttttctaat
     6121 ttaagttaat tttatgtcag atgttgagtt aaacgagcag agcgagcaag agcctgagca
     6181 aaaaccgaaa aagcgaaact atttcctttc aaccttattc aaaatcgggg tgataggagc
     6241 ttgcagtatg gctttttacg gcatttattt agacggtaaa atccgtagca aaatggatgg
     6301 acagatatgg gaattaccag ctgaggtgta tgctcgtatt gaaagcatta aaattgaaga
     6361 caacttaacg cttgagcaaa ccaaacaagc gttgctggat aacggctatc gtcaagtttc
     6421 tcaagttgcg acaccaggcg atttcaaaat tgaagaaaat cagctggtgt tgctacgccg
     6481 agctttcccg tttccagaaa caccggaatc gcaacgagtg cttcgtttaa ctttcaaaca
     6541 agataaatta acttatattg aagatttggt gcagggtaag ttgatcgagg agttccgctt
     6601 ggatccgaaa ttactggcgt tactgcattc cgatagcgat gaagaacgtc aagcattacg
     6661 cttacaacaa taccctcgct tgttaattga gtcactgatt ttaaccgaag atcgccgctt
     6721 ttatcagcac gatgggatta gcccaatcgg tattgcccgt gctttaatca ctaactacca
     6781 agctggcaga acagtgcagg gtggcagtac cttaacgcaa cagttagtga aaaacttgtt
     6841 cttaagtaat gaaagatcgt tggtgcgtaa ggtaaatgaa gcgttaatgt cggtgatttt
     6901 agacttccgt tacgataaaa accgcatttt agaaacctat ctcaacgaaa tttatttagg
     6961 gcagaacggt agctaccaaa ttcacggttt tgcattggca agccaattct attttggtcg
     7021 tccgatccaa gagattagct tagaccaaat tgcgttattg gtgggaatgg taaaaggtcc
     7081 atcactgtat aacccgtggc gaaatccgca aggtgcggta gagcgacgta atgtggtgtt
     7141 gcgattattg ctggatcatc aagtgatcaa tcaagaactt tacgctctgt taagctcccg
     7201 cccgcttaat attcgtgaaa aaggcacgat ttaccgtgag caacctgcct ttatgcaggc
     7261 attaaccatt gatttgaaaa atgagttagg cgaaattaag tcagctcaac tttccggtac
     7321 taaaattttt acgacccttg accgtaaaca gcaacgttca gccgaacaag cagtgattac
     7381 cggtttggaa aatctggaaa gtaaaaataa aaaagtcagc gatttgcaat cggctattgt
     7441 cgtggcggaa taccgcacag gtaaagtgag ggcgatagta ggagaccgct taacccaatt
     7501 tgccggcttt aaccgtgcga ttcagtcaaa acgccaaatc ggttctttag tcaaaccatc
     7561 ggtttatgcg attgccttgg cacagcctga attattccgt ttaaatacac cgatccaaaa
     7621 taaacaaatt tcaattaagg ttggttcaac ctactggaca ccccgtaact acgaccgcag
     7681 ttttagtggc tcggtgatgt taatggacgc cttagtccgc tctctgaata ttccaacggt
     7741 gaatattggt atgaaagttg gcttaaaaaa agtcattgcc aaacaaaaag agatgggctg
     7801 ggataatgcg gaaattccgc cttacccatc aaccttgcta ggttcatatt cgatctcgcc
     7861 ttacgatgta acccgctctt atcaagtaat tgccaacgaa gggctgaaaa caccattaac
     7921 caccattgag gcgattattt cgcacaatgg cgaaatgttc tatcagcgag atagcggcaa
     7981 agtgagcgag caggtgcttc caccggaagc tgcggttcaa acaatgtatg cgatgcaaca
     8041 ggttgtggaa cgaggtacgg ctcgtagctt acaaaacgat tttgccaaac tgaaattagc
     8101 cggcaaaacc ggtacaacca ataacgctcg agatacttgg tttgtgggaa ttgatggcga
     8161 acacgtcacg accgtctggc tgggtaaaga taacaatggg gataccaacc tcaccggttc
     8221 aagtggtgca ttgtatgtgt ataagcaata tcttgagaga gccttgcctc aggcgtatag
     8281 attaccgaaa cttaatacga tgcaatgggt ggggattaat agctatggta gctggaattg
     8341 cgatagtggc agacaaattc cagtttggcg agataaaggg caacgttatt gtaccagtgc
     8401 aggtaatgta gtggctacag aaaaaccaag tgtttgggat gcactttcgc tgaaaaaaga
     8461 aaatatcgag gctaaagcag aagaagctga agggaagcaa ccaatcccta ctgaagaagt
     8521 tgcaccaatt gaataaaaat aaatttgata gccattatca ttcaagacgc acaatctgat
     8581 aggttatacc tataaaaagt atatattata accatttgct agttttcatt ttggattgta
     8641 atatagtaat aaattaaata agcagtatct aatttaattt tttaagatta acaattaaga
     8701 ggagcatatt atggcaatgt caaatatcgg tttagataaa gaaatcgcaa aaaaattagc
     8761 agatgagtta aatggtttat tagctactta ccaagtgttt tacactaatg tacgtggcta
     8821 ccattggaat gtacgtggtg tgaacttctt tgaattacat gcaaaatttg aagaaatcta
     8881 cgatgattta gtgacaaaag tagatgaaat tgcagaacgt attttaacct taggtcacac
     8941 acctcaaaat gcgttcagcc aatatttaac caagtcacaa attcaagaac atattggtgt
     9001 ttcagctgca caagaatgtt tacgtggtac tttaaatggt ttcaaaacat tactgggaca
     9061 acaacgtgaa attcttgctc tagcaagtga tgcaaacgat gaaggtactg cttctcaaat
     9121 gagtgattac atcaaagaac aagaaaaatt agtatggatg ttcactgcag cttgtgctga
     9181 atgttcagac tacgcacaag gttgttcttg taactaagtt gcaataatat aattaacccc
     9241 cgataatgaa agattatcgg gggttacttt ttcagatagc tatgtcgcaa ggatttcaat
     9301 ttaaacagtt ttttattcgg cacgacagat gtgcaatgaa agtcaataca gatggtattc
     9361 tattgggagc gattgctcac ataaaagatt gtcagaatat tttagatctg ggtacaggtt
     9421 ctggtttagt agcacttatg cttgctcaga gaacagatga ttgttgtcac attactgcgt
     9481 tggaacttga accaaatgcc taccaacaag cggtcgaaaa tgcccaaaat tctgcatggg
     9541 caaatcggat ttccgttttg caaggtgatg tgatgcagca ggcatttgaa cagaaatttg
     9601 acttgattgt tagtaaccca ccttattttg cagattcact tgcagctcga accacagaga
     9661 gagatttagc tcgctcaatt agccattaca gccacttgaa ttggctggca aaagcaaaac
     9721 catggttagc agagaacggg aaaattacac tgattttacc tatagcagct gcagaaaaat
     9781 tagttgacca aagtgaacaa attggattgt attgtgttga acaatggtta atttttacca
     9841 agagcggcaa attggctaaa cgcagtatag tgagttttag tttaaacaag caaaagcgtg
     9901 aacataaaga gctgatcatc tatgatctag ataa
//
LOCUS       DANJWC010000084         9914 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.084, whole genome shotgun sequence.
ACCESSION   DANJWC010000084 DANJWC010000000
VERSION     DANJWC010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9914
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.084"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            103..354
                     /locus_tag="PWX02_001484"
     CDS             103..354
                     /locus_tag="PWX02_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729486.1"
                     /translation="MKNKNFEIAKVVILNEQLGSPKDLELKVVENGLSDEDKYHIKQA
                     VLKAVENGCLDPEMLASRCCSAIETINKSRINTGNGSCE"
     gene            complement(357..632)
                     /locus_tag="PWX02_001485"
     CDS             complement(357..632)
                     /locus_tag="PWX02_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729487.1"
                     /translation="MKNNILITYDLNKSGQNYAALIEKIKTLGAWAKVQQSAWYLHTS
                     YSSKEVLEMLSTVTDYNDSLFVAQLSEANWRGLSNEVSKFIQEQWWK"
     gene            884..959
                     /locus_tag="PWX02_001486"
     tRNA            884..959
                     /locus_tag="PWX02_001486"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:917..919,aa:Arg,seq:tct)
     gene            1310..1825
                     /locus_tag="PWX02_001487"
     CDS             1310..1825
                     /locus_tag="PWX02_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013525949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HDL1729488.1"
                     /translation="MDYLLQLLNKLELNTIVGLGTCLFTGYLAFFAWSQLKELRKQHK
                     QKSTIELLISNNSNEYYRKRRKAYMAMRDSGVNLTVLACALNDKNATAEQKRQNFIVL
                     DVLNAIEFICVGIKEDLFDEAVYKRMSKSSVIKDWNTLKPYIMELRRINNNNNKLFCE
                     FEWLATKWESE"
     gene            2092..2802
                     /locus_tag="PWX02_001488"
     CDS             2092..2802
                     /locus_tag="PWX02_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005650531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HDL1729489.1"
                     /translation="MSNQVQFPVFNFNSSAVRVIIDPNQEFWFCGTDVCDVLGYSNAP
                     DALAKHCKPKGIAKRYTPTKGGNQELTFINEPNLYRLIIKSRKPEAEKFEAWVFEEVL
                     PQIRKTGKYTLQNSQQNLPLTLPEKPRKIQGTTRAAEGEYLYTREFSAMEIDHLAKLW
                     DIAEEQHKFIESLTPLFETMQSEQWFKKHSRALNCFNHHNYSYHILSEMCEDVEEWTG
                     IMPSIRAVKMKGKYYSRF"
     gene            2909..3415
                     /locus_tag="PWX02_001489"
     CDS             2909..3415
                     /locus_tag="PWX02_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729490.1"
                     /translation="MEAKYNMIIELAQQQGLTHIQLTQKHCLHLAASVGKLCSGVVNN
                     AKQKIKTATGEIFTLLTIINHQIQFDEDLELHTWKASGTTPPEQRAIRMLHHTSQFAW
                     FAPWLVDSNEKEQNQNTIRINLFNKCSGILIELNGILKEYELTLDECLDEFLKNKHCL
                     NEFSIHKE"
     gene            3419..3778
                     /locus_tag="PWX02_001490"
     CDS             3419..3778
                     /locus_tag="PWX02_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729491.1"
                     /translation="MTTFSIYTSVWHDSSTKGFRHNIKHKRHDCWRADIRIKQKINDV
                     WVQISRIRKRFKCPEQAKSFVEQWKQIDSPAFMQHQINRQALREELSNLEQRKIQKNG
                     MLEVIEYRISQIKELLK"
     gene            3775..4254
                     /locus_tag="PWX02_001491"
     CDS             3775..4254
                     /locus_tag="PWX02_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729492.1"
                     /translation="MKRNKVESYTIRLLKNNHTKRYFGEVILNGIKEISTAEMMSEIN
                     AIKALNKRIEALNKTKAVNLPNYPEIRGHQPIMYNYTFEQIEEDPEQVKPKPSKPKRK
                     PFTPYGLNGYFVDSNGNIRLHLDRRASAETITLETDFFNALAEMVKRTQEANNAKHA"
     gene            4388..4600
                     /locus_tag="PWX02_001492"
     CDS             4388..4600
                     /locus_tag="PWX02_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729493.1"
                     /translation="MIGYEDYTDASLQPEEDPSEEAFLLVEEKIIDVLENNGFETVAT
                     ILAGSDGWCNAINAYLAEQNKHRNEF"
     gene            4613..5536
                     /locus_tag="PWX02_001493"
                     /pseudo
     CDS             4613..5536
                     /locus_tag="PWX02_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251566.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
     gene            5529..6191
                     /locus_tag="PWX02_001494"
     CDS             5529..6191
                     /locus_tag="PWX02_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017806850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation protein TolB precursor"
                     /protein_id="HDL1729494.1"
                     /translation="MYKFKPRCSMLHQLMSEPRTKADREAGLISDTAKAAVREVAKFD
                     LFGYQSFDGNKYTEKGNQLEEQAIKLSGFTRGLVLKKNTERRENDWISGECDIYVPSR
                     KLIIDTKCSWDIGTHPFFADEAIAKAEKAGYDIQMQGYMWLWDCEEAQIDFVLFPTPI
                     DLIGTYESTEKLIDLVEQIPQQKRITTVTVKRDEAIIAKIKDRVKAASKYYDKLIQEA
                     MN"
     gene            6232..6498
                     /locus_tag="PWX02_001495"
     CDS             6232..6498
                     /locus_tag="PWX02_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729495.1"
                     /translation="MTTQKKYELLQNDTINHNGRTLYRIKAITTFGTVFAGTLGGYIE
                     KESNLKHLGNAWVYDNAEVHDEKTKREYQLLIEVAKSRILGEAT"
     gene            6526..6978
                     /locus_tag="PWX02_001496"
     CDS             6526..6978
                     /locus_tag="PWX02_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL1729496.1"
                     /translation="MKPFDLNKALAGEPVKLRNNDKAFVKYLISDDYIRDNKDHQVQG
                     YTVDEENVFLSEVSWAVSGSHFNDGTIAQYDIVGMWEEPRPTVTLTLPCPLKEPRDGM
                     WFIGDNFNVIKSNFPTHSYIEKLFDQGLYFASAEDARAWLDALKNSMR"
     gene            7077..7295
                     /locus_tag="PWX02_001497"
     CDS             7077..7295
                     /locus_tag="PWX02_001497"
                     /inference="COORDINATES: protein motif:HMM:NF016343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HDL1729497.1"
                     /translation="MNEENNGWISVKDRLPEIRKEVLITNNFIMIAQFNGESWFKPGG
                     FLGIQPVYGVTHWQPLPEPPKEKANERN"
     gene            7506..7730
                     /locus_tag="PWX02_001498"
                     /pseudo
     CDS             7506..7730
                     /locus_tag="PWX02_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248793.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            7990..8826
                     /locus_tag="PWX02_001499"
     CDS             7990..8826
                     /locus_tag="PWX02_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HDL1729498.1"
                     /translation="MANSVMIVQGVEVKVTARDGEDYINLTDMLRAKDGDFFISDWLR
                     NRNTLEYIGIWEEMNNPNFNYGEFATIKNQSGLNRFKISVKEFVAQTNAISLQAKAGR
                     YGGTYAHKDIALEFAMWISPEFKLYLIKEFQRLKQQEANESKLEWSVKHILSKANYRI
                     HTDAIKDHIIPALLNTKQHTFVYSSEADILNQALFGQTAKQWKDRNPNLKGNMRDHAT
                     VEQLTVLAALESQNALLIEQGYSQEERLAMLNRLAIQQMSSLLQTRAIEELKEKPLLI
                     EE"
     gene            9212..>9914
                     /locus_tag="PWX02_001500"
     CDS             9212..>9914
                     /locus_tag="PWX02_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HDL1729499.1"
                     /translation="MTTLTFQNTTLSVIDQNNQKWMPALEVGRALGYKNPSSDISKLY
                     ERNKDEFTPSMTAIIDMDTASGMQKVRIFSLRGCWLLGMRSHTKVAKDFRKWVLDILD
                     KEVLQNNQQIAPLAEPTITAEEQNMLQNAVKATHERTKLSYGEIWARTKNKFRVAEYK
                     QIKRSDLRDALIYVASMQPDFLTQPKQTINVDWFNMMNFALLLKQYHRFTKDLPHIHT
                     YLSALPEHSAMAGLLK"
BASE COUNT         3411 a         2018 c         1979 g         2506 t
ORIGIN      
        1 aacgcagctc taaaattgcg tgacagctcg gagagacggc atttcaaatt ccaaagcata
       61 tttgagaaag tgtgttttgg aatatttaac tagaggtaaa ttatgaaaaa caaaaatttt
      121 gaaattgcga aagttgttat tttaaatgaa caactaggaa gccctaaaga tttggaatta
      181 aaggttgtag agaatggcct gtctgatgag gataaatacc atatcaaaca ggctgtactt
      241 aaagctgtag aaaatggttg cttggaccca gaaatgcttg catcacgatg ttgttctgca
      301 attgaaacaa tcaataaaag cagaataaat acaggtaatg gaagttgcga ataggtctat
      361 ttccaccatt gttcttgaat aaatttacta acctcattgg ataatcctct ccaattagcc
      421 tcggataatt gagcaacaaa aagagagtca ttgtaatcag ttacggtgct tagcatttct
      481 aaaacctctt ttgaggaata tgaagtatga agataccaag ctgactgctg tactttagcc
      541 caagctccaa gagttttaat tttttcaatg agtgctgcgt aattttgccc tgatttattt
      601 aaatcataag taattaaaat attatttttc ataacttaat ccttatttga gttgtgagag
      661 acaataagga aacttgactg ctcacaagta taaaagagcc accatctcaa accgctctca
      721 acaaagagtt agcccgataa tcacactttg gttttttgcg tatgttttcc caaagtgccg
      781 ttatccagcg aaacgaatgg actagagagc ggtttgagat ggtgaaagcc atcctcctta
      841 gattgaagta aaccctgccg ttggggttaa gcaacggtac tttgaccgca tagttcagtg
      901 gatagagcag ctgccttcta agcagtgggt cgaaagttcg aatctctctg cggtcgccat
      961 ccaaaaccgc tttacgcaca tcatcgatga cgagcctacg aagtggaaac ctaccgtgta
     1021 gagcagtttt gaatggctaa gttgtgtaac tgctacaact taaaaagagc ggttaaaagg
     1081 tttgacatca tgacggtgtc ggattaatat aacgtcatta aatattattg acagtaatgc
     1141 tgacaataat tgtttgttct ttgtaataat ttagatgaca aaacagctta ccaagggaac
     1201 ttgagtaggc ttttttcgtc taagttacaa taatcttcat catctaggag tgaaatatga
     1261 aaaatttagc taactttaca ttaattgcta atgagggctg ggaggtttaa tggattacct
     1321 tttacagctt ttaaataaat tagagctgaa cacaatagtc gggctaggta cttgtttatt
     1381 tactgggtac ttagcctttt ttgcgtggtc tcagcttaaa gagcttcgca agcaacataa
     1441 gcaaaaatcg accattgaac tccttatcag caataacagc aacgaatact accgtaaacg
     1501 cagaaaagcc tatatggcta tgcgtgatag cggtgtaaac cttactgttc tagcgtgtgc
     1561 attaaatgat aaaaatgcca cagcagagca aaaaagacag aatttcattg ttttagatgt
     1621 gcttaatgcg attgaattta tctgtgttgg tatcaaggaa gacttatttg atgaagcggt
     1681 atataagcga atgagcaaaa gcagtgtcat taaagactgg aacacgctca agccctacat
     1741 aatggagctt cgcagaatca acaacaataa caacaagctc ttttgtgaat ttgaatggct
     1801 tgcaacgaaa tgggaatctg aatagcctca tatattattg aggctttttt gttgacaacc
     1861 gccaaacttc ggattaagat aaccgcacta ctaaacgaaa gtcggttatc cgctccgaca
     1921 aaacagcggt ttttttgtac ccaaaattta ggaaatgccc taaaactatg gcgggtcgag
     1981 agagcctaat agaacacccg aaaggggaat aagctccgcc gatctttcgt ttggtagttg
     2041 aagcccgcca cctactaagt ggcattaact aaaaacgaaa ggtacaaaat tatgtcaaat
     2101 caagttcaat tccctgtttt caatttcaat tcatctgctg ttcgtgtcat tatcgatcca
     2161 aatcaagaat tttggttttg tggcacagat gtctgtgatg ttttaggtta ttcaaatgct
     2221 cctgatgctt tagcaaaaca ctgtaagcca aagggtatcg caaaacgcta tacccctaca
     2281 aaaggcggaa atcaggaatt aactttcatc aacgaaccaa acctataccg actgattatc
     2341 aaatctcgca aacccgaagc ggaaaaattt gaagcgtggg tatttgaaga agttctgcca
     2401 caaattcgca agactggtaa atatacgttg caaaattctc agcaaaacct accgcttacc
     2461 ttgccggaaa aacctcgcaa aattcaaggc acaacccgag cagcagaggg tgaatatctc
     2521 tacaccagag aattttccgc aatggaaatc gaccacctag ccaaactttg ggacatcgcc
     2581 gaagaacagc acaaattcat cgaaagtctc acgccattgt ttgagacaat gcagtctgaa
     2641 caatggttta aaaagcatag cagagcatta aactgcttta accaccacaa ctattcctat
     2701 catatcctca gcgaaatgtg cgaggacgta gaagaatgga caggcattat gccttccatt
     2761 cgtgccgtaa aaatgaaagg aaaatattac agccgattct aattctcctt tcccctccgc
     2821 aactcacttt gcgtaatcac tccgtaattc ctgcggaggg gctttttaca cctaaaattc
     2881 agatttttga ctaaaaaagg aacgaaaaat ggaagctaaa tacaacatga ttattgagct
     2941 ggcacaacaa caaggcttaa cccatattca acttactcaa aaacactgtc ttcatcttgc
     3001 tgcatcagtg gggaaactat gttctggagt cgttaataat gcgaaacaga agattaaaac
     3061 ggctacggga gaaattttta cattactcac catcattaac catcaaatcc agttcgatga
     3121 agacttagag cttcacacgt ggaaagctag tggaacaacc ccacccgagc aaagagcgat
     3181 acgtatgtta catcatacct cgcaatttgc ttggtttgct ccttggttag tggacagcaa
     3241 tgaaaaagaa caaaaccaaa atacaatcag gattaattta tttaacaaat gctctgggat
     3301 tctgattgaa ctaaatggca ttcttaaaga atacgagcta actcttgatg agtgcttaga
     3361 tgaattcttg aaaaataagc attgtttgaa tgaatttagt attcacaagg agtaaaaaat
     3421 gacgacgttt agtatttata ccagtgtatg gcacgacagc tccacgaaag gctttcgcca
     3481 caacattaag cataaacgcc acgattgctg gagagcggat atccgcataa agcaaaaaat
     3541 caatgatgtg tgggtgcaga tttcaagaat acgcaaacgc ttcaagtgcc ctgaacaggc
     3601 aaaatccttt gtagaacaat ggaaacaaat tgattcccct gcatttatgc aacaccaaat
     3661 caaccgccaa gcattaagag aagaattatc aaaccttgag caacgtaaaa tccaaaaaaa
     3721 cggaatgctt gaggtgattg agtatcgtat tagccaaatc aaggagttac tgaaatgaaa
     3781 cgcaataaag tagaaagcta tactatccgc ttattaaaaa ataatcatac aaaacgctac
     3841 ttcggagaag ttatattaaa cggcataaaa gaaatcagca cggcagagat gatgtctgaa
     3901 attaacgcta ttaaggcatt aaataagcgt attgaagctc tgaataaaac caaagccgtt
     3961 aatttgccta attatcctga aatcaggggt catcagccta ttatgtacaa ttacactttt
     4021 gaacaaattg aggaagatcc tgaacaggtt aagcctaaac catcaaaacc aaaacgcaaa
     4081 cctttcacac cctacgggct aaacggctac tttgtggata gcaacggcaa tattcgcctg
     4141 cacctagaca gacgagcaag tgccgaaaca attacccttg aaactgattt cttcaacgct
     4201 cttgccgaaa tggtaaaacg tacacaagag gcaaacaatg ctaaacatgc ttagacaatg
     4261 gattacatac ggcttatacg ccctgctttt tatggttgcc atcaccctcc taatgcaatg
     4321 aagagcttat ccctgaaagc acctgcaagc ctgaaacttg cgaatttaaa gtaacggaga
     4381 aagtgtgatg ataggatacg aagactacac agatgcttca cttcaaccag aagaagaccc
     4441 tagcgaagag gctttcctct tagtggagga gaaaattata gatgtgctag aaaacaacgg
     4501 ctttgaaacc gttgctacta tcctagccgg ttctgatggc tggtgtaacg caattaatgc
     4561 ctacctggcc gaacagaaca aacacagaaa tgaattttag aggaataaga taatgacaaa
     4621 ccaaaatcaa caagtagcac aaaagcaaga taaattcccg attaaaaccc tatttgaaag
     4681 cccgaatgta aagcaaaaac tacaagagct tttaaacaaa aacgcagcca cgtttacaac
     4741 aagcgtgttg cagattgtga atagtaactc aatgttacgc aatgccgaac cgatgagcgt
     4801 attcaacgcc gcttgtatgg cagcaacgct taatttggca ttacaaaacg gcttaggttt
     4861 tgcctacatt gtgccgtacc aaaaccgcaa agagaaaaaa accgaagctt aattccaact
     4921 tggctacaaa ggtttaattc aactggctca acgtagcggg caatttaaac gccttgttgc
     4981 cgtgccggtg tatgaaaaac aactgcttgc cgaagacccc atcaacggtt acgagttcga
     5041 ttggaaacaa aaaccggcta aagatgaaaa accgattggc tattacgctt atttcaaact
     5101 gataaatgaa tttaccgccg aaatctatat gaccacgcaa gaagtccacg accacgccaa
     5161 ccgctacagc caaacctacc gcaaaggcta cggcgtatgg cacgacaact ttgaggcaat
     5221 ggctctcaaa actgtgatga agctcttgct ttccaaacaa gctccattat cagtggaaat
     5281 gcaacaagcg gtcttggcag atcaagcggt cgtaaaagat gttgaaaatg cagaatttgc
     5341 ctaccctgac aacgaaatcc aagaggcgga atttaccgaa ctcaaggtca gcgatgaggt
     5401 gtttgagaaa tgcaaacaaa ccatcatcaa caaagaaagc accttgcaag atatgtgcga
     5461 taacggctac gagttcaaca aagagcaata tgcggagttg gagaagttag aaaatgaaca
     5521 gcaatcaaat gtataaattc aaaccccgtt gctcaatgct ccaccaacta atgagcgaac
     5581 ctcgcaccaa agccgaccgt gaagccggac tgatttccga caccgcaaaa gcagcggtgc
     5641 gtgaagtggc taaatttgac ctattcggtt atcaatcctt tgacggcaac aaatacactg
     5701 aaaaaggcaa tcagctagaa gaacaagcca ttaagttaag cggttttact cgtggcctgg
     5761 ttctaaaaaa gaacaccgaa cgccgagaaa acgattggat ttcaggcgag tgtgatattt
     5821 atgtaccaag ccgaaagctg attatcgata caaaatgcag ttgggacatc ggcacgcacc
     5881 cgttttttgc agacgaagcc atcgccaaag ccgaaaaagc cggatatgac attcaaatgc
     5941 aaggctatat gtggctgtgg gattgcgaag aggctcaaat cgattttgtg ttattcccta
     6001 ctccaatcga ccttatcggt acttacgaaa gcacagaaaa gctgattgac cttgtggaac
     6061 aaatccccca acaaaaacgc attaccaccg tgacggttaa gcgtgatgaa gccatcattg
     6121 ccaaaatcaa agacagagtg aaagcagcca gtaagtatta cgacaagctt atacaagaag
     6181 ctatgaatta attcaatcgc cccgaaaggg gctttttaat aggtagccaa tatgacaacc
     6241 caaaagaaat acgaactact ccaaaacgac acaatcaacc acaacggcag aacactctac
     6301 cgcatcaaag ccataaccac atttggaaca gtctttgcag gtacacttgg cggctatatt
     6361 gaaaaagaga gcaacctaaa acatttagga aatgcttggg tgtatgacaa cgccgaagtt
     6421 cacgatgaga aaacgaagcg ggaatatcag ctactgattg aggtagcaaa aagcagaatt
     6481 ttaggagaag cgacttaatc agtcgctttt ttattggaga ataaaatgaa accatttgat
     6541 ttaaataaag cattggcagg tgagccagta aaactaagaa ataatgataa ggcatttgta
     6601 aaatacctaa ttagtgatga ctatattaga gataataaag accaccaagt acaagggtat
     6661 acagttgatg aagaaaatgt atttttgtca gaagtatcgt gggctgttag cggatctcat
     6721 ttcaatgatg gaacaatagc tcaatatgat attgttggta tgtgggaaga accaagacct
     6781 acagtaactc tcactcttcc ttgtccatta aaagaaccaa gagatggaat gtggtttatt
     6841 ggcgataatt tcaatgtgat aaaatcaaat ttccctacac attcttatat agaaaaacta
     6901 tttgatcaag ggttatattt cgcttcagca gaagatgcta gagcgtggtt agatgcactt
     6961 aagaatagca tgagatagtg tgcttattat tctctcaacc attggtattt tctcgtccgc
     7021 atacttgtac gagaaatata aaacatcaag aattattgtt attttggaga aagaaaatga
     7081 atgaagaaaa caacggctgg atttcagtca aagacagatt gccagaaatc cgcaaagaag
     7141 tcttgataac aaacaatttt attatgattg ctcaattcaa tggcgagagc tggttcaagc
     7201 cgggtggatt cttaggtatt cagcctgtct atggtgttac ccactggcaa cctcttcccg
     7261 agccaccaaa agagaaagca aatgaaagaa attaaactag gtgtatcgcc actcacaaat
     7321 gaaatttatg caggctatgt caataaaaaa ggcgatttat gaactgaaaa gcaagatgtg
     7381 acaatgccgg ctctattatg tgtagttcaa cactgtttga agtttggctc cccaatcatt
     7441 atctcaaaag aaaatggcga gcctgaattt gaaattactg taaaggattt aagaaaatga
     7501 gtaaagaaaa caacggctgg atttccattg aggacaaatt accacctatt gaaacggatg
     7561 tgctagggtt atgcgatatt tcaggaatgc agttaatttt aatagtttca agagaacttg
     7621 ccgataacga gtggtatttt cttagtgtaa atcaatatgg gcttgatgac gatgtgattg
     7681 cagtaaccca ctggcaacca cttccaaagc tgccaaagga caaccaatga cagacaccaa
     7741 acgattagat aaactaaaca ttaaaggttt cctaaatgaa aaacaaactt aagcgtattt
     7801 tacacaaaat caaaactgag caagatatga atttcatttt ttggttatgt gttgtagttc
     7861 tactctttaa cttggtccca tacttatacg gattaatcta attgacaccg cccccaattc
     7921 ggattaagat aaccgcactt tcaagccgta tttcacggct ttttttgtac ctaaaaacgg
     7981 agaaacgaaa tggcaaatag cgtaatgatt gtacaaggcg tggaggtcaa agtaaccgct
     8041 cgtgatggcg aagattacat caaccttact gatatgctac gagccaaaga cggcgatttc
     8101 ttcattagcg actggttacg caaccgcaat acccttgaat acatcggcat ttgggaagaa
     8161 atgaacaacc cgaattttaa ttatggcgaa ttcgccacaa ttaaaaatca atccggctta
     8221 aaccgtttca aaatcagcgt caaagaattt gttgctcaaa cgaatgcgat tagcttgcaa
     8281 gccaaagcgg ggcgatacgg tggcacttat gcccataaag acatcgctct tgaatttgcg
     8341 atgtggatta gccccgagtt caagctctat ctcatcaaag agttccaacg cctcaaacag
     8401 caagaagcga acgaaagtaa gctcgaatgg agcgtaaaac acattttaag caaggcgaac
     8461 taccgcattc atactgatgc catcaaagat cacatcatac cggcattgct gaatacaaaa
     8521 caacatactt tcgtttattc cagcgaagcg gacattctca atcaagctct cttcggacaa
     8581 acggcaaaac agtggaaaga ccgcaatccg aacctgaaag gcaatatgcg agaccacgct
     8641 accgttgagc aacttaccgt ccttgctgca ttggaaagcc aaaacgccct actaattgag
     8701 caaggttaca gccaagaaga gcgactggca atgctcaacc gtttggctat tcagcaaatg
     8761 agctcgctgt tacaaaccag agcgattgaa gaactgaaag aaaagccatt gctaattgaa
     8821 gaataaaatc taattgacaa aaaccgccct ttcggctaag ataaccgcac tttcaacagt
     8881 aaagtcggga actgcaattc ctgaatacca tacgagcggt gaagaatgat agtcgctcaa
     8941 tgcgactttt tttatagccg gaatctagtc aaatcaaggc actgtcaatt tgacagtacc
     9001 cctaaataag gcacttccaa tttggaagta ccccctaaaa ctaggcgctt gcaatttgca
     9061 aatcccctat gttgtcaatg atgggctagg taaggagatc gaaagatctg ccgttgtctc
     9121 gtatggacgg ttattgcaga ccttgcctag ttcatcacca gtactgcaat actcgtgatg
     9181 agtttttaaa cttacatatg agaaattcag aatgacaaca ttaactttcc aaaacactac
     9241 actttccgtt atcgatcaga ataatcaaaa atggatgccc gccttagaag ttggcagagc
     9301 gttaggttat aaaaatccat cgtctgatat atctaagctg tacgaacgca acaaagatga
     9361 atttactcca tcaatgacag ccatcattga tatggatacc gctagcggta tgcagaaagt
     9421 cagaattttc agcttgcgtg gttgctggct attaggtatg cgtagccaca ccaaagtcgc
     9481 caaagacttc cgcaaatggg tgctggatat tttagataaa gaagttttgc aaaataatca
     9541 acaaatcgca ccgcttgcag agccaacaat cactgccgaa gaacagaata tgctacaaaa
     9601 tgcggtgaaa gccacgcacg aacgcaccaa gctcagctac ggtgaaattt gggcaagaac
     9661 caaaaacaaa ttcagggtgg ctgagtacaa gcaaatcaaa cgtagcgatt tacgagatgc
     9721 tctgatttat gtagccagta tgcaacccga ttttctcaca caaccaaaac agacaatcaa
     9781 tgtggattgg ttcaatatga tgaacttcgc cttgctactg aaacaatacc accgtttcac
     9841 taaagattta ccgcatatcc acacctactt gtcggcactg cccgagcata gtgcaatggc
     9901 aggattgctg aaaa
//
LOCUS       DANJWC010000085         9858 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.085, whole genome shotgun sequence.
ACCESSION   DANJWC010000085 DANJWC010000000
VERSION     DANJWC010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9858
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.085"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            210..1508
                     /gene="tig"
                     /locus_tag="PWX02_001501"
     CDS             210..1508
                     /gene="tig"
                     /locus_tag="PWX02_001501"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDL1729500.1"
                     /translation="MSFSIETLEGLKRRVTINVPADKIEAAYKEQLKGYAKNARVDGF
                     RKGKVPHSIIEQRFGIAARQDVLSDEMQRAFFEAVIAEKINLAGRPTFTPNNYQPGQD
                     FSFTAEFEVFPEVELKGLENIEVEKPVVEIADADLDKMVDVLRKQQATWSESQEAAKA
                     ESRVTIDFVGSVDGEEFEGGKASDFVLFMGQGRMIPGFEDGIVGRKAGEQFDIDVTFP
                     EEYHAENLKGKAAKFAITLKKVEEMVLPELTEEFVKKFGNAKSVEELRAEIKKNMQRE
                     LKNAVTSRVKTQVINGLIAQNDVEVPAAAVEEEINVLRQQAVQRFGGKPEMAAQLPAE
                     LFEAEAKRRVQVGLLLSSIIGSNELKVDEKRVEEMIADIASAYEQPAEVVAHYAKNRQ
                     LTENIRNVVLEEQAVDAVLAKAKVTEKTMSFDEVMSQQAQ"
     gene            1624..2076
                     /locus_tag="PWX02_001502"
     CDS             1624..2076
                     /locus_tag="PWX02_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL1729501.1"
                     /translation="MEQLTFAQQLEQFFANHTLMVFAWVALLIAVIFSFYKSATQKYK
                     IVDNAQATQLINKEEAVVVDLRSDEEFRAGHIIDSIHVLPSDIKAAKINQIDKYKARP
                     VIVVDTNGFTSGGVAADLAKQGFSRVYVLKEGIGGWRAASLPTIKKHK"
     gene            2097..2597
                     /gene="secB"
                     /locus_tag="PWX02_001503"
     CDS             2097..2597
                     /gene="secB"
                     /locus_tag="PWX02_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HDL1729502.1"
                     /translation="MSEENQVASTEEATQTPFELQIQRIYVKDVSFEAPNLPTIFQQE
                     WKPQLGFELDTETKEIGEDLYEVTLHITVQTTLEDSNDVAFICEVKQAGVFTIKGLEG
                     IQLAHCLASQCPNVLYPYARELISSLVNRGTFPALNLSPVNFDALFMDYLQRQEESAE
                     EPALAN"
     gene            2704..3714
                     /gene="gpsA"
                     /locus_tag="PWX02_001504"
     CDS             2704..3714
                     /gene="gpsA"
                     /locus_tag="PWX02_001504"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL1729503.1"
                     /translation="MSEIYSAPITVLGAGSYGTALAIALSRNGHKTYLWGHNPDKMAV
                     LASERMNQDFLPDIVFPEALEIVADLATAVQKSKDLLIVVPSHVFSEVLAQIKPFLSQ
                     DHRIMWATKGLERDTGRLLQTVAKEILGEIYPLAVLSGPTFAKELAAGLPTAISLAST
                     DPVFADQMQKRIHCSKAFRVYLNNDIIGVQLGGAIKNVIAIGAGISDGMGFGANARTA
                     LITRGLAEISRLGVSLGADPKTFTGMAGLGDLVLTCTDNQSRNRRFGLALGQGKSAEV
                     AIAEIGQVVEGFYNTKEAYLLAQTQGVEMPIVEQIYQMLFCGKQASDVVKTLLGRERK
                     GE"
     gene            3730..4545
                     /gene="cysE"
                     /locus_tag="PWX02_001505"
     CDS             3730..4545
                     /gene="cysE"
                     /locus_tag="PWX02_001505"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDL1729504.1"
                     /translation="MQQSEINQIWQAIRDEAKELANCEPMLASFFHATILKHHNLGDA
                     LSYILANKLENPIMPAIALKEIIEEAYRAEPQIIASAACDINAVRTRDPAVDKWSTPL
                     LYLKGFHALQSYRVTHYLWNQGRKALAVYLQNEISVAFDVDIHPAAKVGCGIMFDHAT
                     GIVVGETSVIENDVSILQGVTLGGTGKENGDRHPKIREGVMIGAGAKILGNIEIGRYS
                     KIGANSVVLQPVPDHATAAGVPARIIGKSSEQKPAFDMNQYFEDEQGLFGDGI"
     gene            4594..6771
                     /locus_tag="PWX02_001506"
     CDS             4594..6771
                     /locus_tag="PWX02_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S6"
                     /protein_id="HDL1729505.1"
                     /translation="MQLTFIQMFCPLLSGLTIFTICYVFPIVVIECLFCHQLMLEFAK
                     FYFLDDKYMKPASFKLSLISAFVGLSLAQLSYSSTVRGDIDYQYFRDLAENKGKFYVG
                     AKNVEIFNKQGDKIGIALENIPMIDFSVITRGGGYATLTKPQYIVSVEHNRGYRTVQF
                     GDSGSENPDNHHFNYQLVSRNDYDPKGSKAIQGFTHNDYQTPRLHKLVTETAPAEQSQ
                     EKNYDLYRDASIFPMFIRAGSGTQYILAEKNAVKEKLADPYAYLIGGTTLKLTSNNAN
                     TFVFKGNVFDDSYSPLSSHPQQGDSGKTFTWTALGNSSRITGATAELVVDLADTSLPD
                     PTTGKPHKPSLHHGKTLKISGDGNTLNLANNINQGAGALYFSGNAVVTGANEMTTWLG
                     AGISVDKNKNVEWQVHNPVGDRLSKIGEGTLTVSGKGKNLGSISVGDGTVILNQQAGE
                     NGEKSAFSEVGIVSGRPTVILNSADQVDPNSIYFGYRGGRLDLNGNSLTFNRIQNVDD
                     GARIVNNNAGTAANISLVGQVFTANDVRTINFGEGYNADLFRSQGAYFVLENNAWKFI
                     SWNHDDAKKYVVEKKNKALENQLYAYNGYFGESDSSRENGKLNIHFKPINAGGKLLLT
                     GGTTLDGILSAENDAEIILSGRPIPYARQVLDENKKTINKEIIKEGEWINRTFSATTF
                     QAKDNGKIEVSRNVVEVNGNFNLSDNATAQVGFTQGKSQSCVR"
     gene            6784..7215
                     /locus_tag="PWX02_001507"
     CDS             6784..7215
                     /locus_tag="PWX02_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopropylmalate isomerase"
                     /protein_id="HDL1729506.1"
                     /translation="MASCNLSVLSDKDLHSWQRTTVNSDIKLTQNARFELGSKADLIG
                     TIESNGDSQINLRNGSSWVMTGNSNVNKLNVDNATITLDNNVGEPNTLNINSLSGSGV
                     INFITYFAQTISDLINVEHASGAFKAKISQIGTPTTQRGVL"
     gene            7212..7364
                     /locus_tag="PWX02_001508"
     CDS             7212..7364
                     /locus_tag="PWX02_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HDL1729507.1"
                     /translation="MIFYKYINLKKASHLNFKVAKYIDEQGKSRKMYLMTQDGFTLLV
                     MGFTHK"
     gene            7361..7600
                     /locus_tag="PWX02_001509"
     CDS             7361..7600
                     /locus_tag="PWX02_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729508.1"
                     /translation="MNRTFALNKIAVERCKQDFPDCYHFKKLMAEECTELADRLKMGL
                     VLMAELKTTSTLTSDQNRHFKAFRNGAKYLIKQLE"
     gene            complement(7662..8849)
                     /locus_tag="PWX02_001510"
     CDS             complement(7662..8849)
                     /locus_tag="PWX02_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL1729509.1"
                     /translation="MARKITPLNDTQIRKAKPEDSPLRDGNGLLLVITSNSKLWRFRY
                     ERPFTKKRNDLSIGAYPDISLAQAREIREEYRALLAQNIDPQTHRQQQEQAKANEISN
                     TYENVAWAWLEHRKTKKNFSDNYQKDVIALIKNNLLPHFGRMPITQITAPMAIKAFKQ
                     YQDEGKLEKLKRAIQKHNEIMMFAVHREMIPFNPTANIAKEFDSPTVEHFKTIKPEDL
                     GEFLYTLQNAQIHLQTRYLILWQLLTMTRPNEAAKAKWADIDEKAKLWTIPAENMKRG
                     IEHKITLSRQAIALLREIKKLSGGKTYLFPSVKNPQTHVNTQTANGAIKRMGYKGKLV
                     AHGLRAIASTYLNEKGYNSDLIEVALSHLNSDRIKAAYDRGERLEQRFKLLQVWADFP
                     PLV"
     gene            complement(9047..9413)
                     /gene="ssrA"
                     /locus_tag="PWX02_001511"
     tmRNA           complement(9047..9413)
                     /gene="ssrA"
                     /locus_tag="PWX02_001511"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            9579..>9858
                     /locus_tag="PWX02_001512"
     CDS             9579..>9858
                     /locus_tag="PWX02_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599455.1"
                     /note="binds with the catalytic core of RNA polymerase to
                     produce the holoenzyme; this sigma factor is responsible
                     for the expression of heat shock promoters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HDL1729510.1"
                     /translation="MKKFNENEIEDAEIIDAEPIDVDEIDEEETSVEAMHPALVSGSM
                     PVPQGNLDSYIRMANQYPILTAEQEKELAERYYYDEDVEAAKQLILSHM"
BASE COUNT         3001 a         1797 c         2206 g         2854 t
ORIGIN      
        1 tgattaagct agccatatca tttcatttgc aaaaatcggt gaaaaaagac cgcttgtagg
       61 caagcccatt gacaagcggt cagattttag ggatttttta caaaactgtt tatttgcgaa
      121 tgagtttctt ctcgacagtt tcttgataga acgctacaat aaccgtctat ttgtatatct
      181 tttttatatc cctaaaccga ggtttaagaa tgtcattttc aattgaaaca ttagaaggct
      241 taaaacgccg tgttactatt aacgtaccag ctgataaaat cgaagcagca tacaaagaac
      301 aattaaaagg ctatgcgaaa aatgctcgtg tagatggctt ccgtaaaggt aaagtgccac
      361 actcaattat cgaacaacgt tttggtattg cagcccgtca agatgtgtta tctgatgaaa
      421 tgcaacgtgc attctttgaa gccgtcattg ctgaaaaaat caacttggca ggtcgcccta
      481 ctttcacgcc aaacaactac caaccaggtc aagacttcag ctttactgcg gaatttgaag
      541 tattcccaga agtggaatta aaaggtttag agaatatcga agtagaaaaa ccggttgttg
      601 aaatcgcaga cgctgattta gacaaaatgg tagatgtatt acgcaaacaa caagcgactt
      661 ggtcagaaag ccaagaggct gcaaaagccg aatctcgtgt aactattgat ttcgttggtt
      721 cagtagatgg tgaagaattc gaaggcggta aagcatcaga cttcgtgtta tttatggggc
      781 aaggtcgtat gattccgggg tttgaagacg gaatcgtagg tcgtaaagcg ggtgagcaat
      841 tcgacattga tgtgactttc ccagaagaat accacgctga aaacttaaaa ggcaaagcag
      901 cgaaatttgc aattaccttg aaaaaagtag aagagatggt attaccggaa ttaaccgaag
      961 agtttgtgaa aaaattcggt aatgcaaaat cagtggaaga attacgcgca gaaattaaga
     1021 aaaatatgca acgtgaactt aaaaatgcag tcacttcacg tgtgaaaact caagtaatca
     1081 acggcttaat tgctcaaaat gatgttgaag tgccggctgc tgcggttgaa gaagaaatca
     1141 acgtattacg ccaacaagcg gtgcaacgtt ttggtggtaa gcctgaaatg gcagctcaat
     1201 taccggctga attatttgaa gcagaagcta aacgccgtgt tcaagtgggc ttattattat
     1261 cttcaatcat cggttctaac gaattaaaag tagatgaaaa acgtgttgaa gagatgatcg
     1321 cagatatcgc ttcagcttac gaacaaccgg ctgaagtggt tgctcactat gcgaaaaacc
     1381 gtcaattaac tgaaaatatc cgcaatgtgg tattagaaga gcaagcggtt gatgcagtgc
     1441 tagcgaaagc gaaagtgact gaaaaaacaa tgtcatttga cgaagtaatg tcacaacaag
     1501 ctcaataatt tccttataag gtgggctttt gctcaccttt tattactttt acaagcggtt
     1561 aaatttagtg ataaatttgc atcaaaacga ccgcttattt tttgatgtta aggaaattga
     1621 attatggaac aactaacttt tgctcaacaa ttagagcaat tttttgccaa tcataccttg
     1681 atggtgtttg cttgggttgc attattgatt gcggtaattt ttagctttta taaaagtgca
     1741 acgcaaaaat ataaaattgt cgataacgca caggctacac agctgattaa taaagaagag
     1801 gcagtagtgg ttgatttacg ttctgatgaa gagttccgtg ccggtcatat tattgatagc
     1861 attcacgtgt taccgtcaga cattaaagca gctaaaatca atcagattga taaatacaaa
     1921 gctcgcccgg taattgtagt agatactaat ggcttcactt caggtggtgt tgcggcagat
     1981 cttgctaaac aaggctttag cagagtttat gtattaaaag aagggattgg cggctggcgt
     2041 gcagcaagcc taccaactat taaaaaacat aaataatcta ttattaagga aattttatgt
     2101 cagaagaaaa tcaagttgca tcaacagaag aagctacaca aacccctttt gagcttcaaa
     2161 ttcaacgcat ttatgtaaaa gatgtttcat ttgaagcacc aaatttaccg actattttcc
     2221 aacaagagtg gaaaccacaa ttaggctttg agttagatac tgaaaccaaa gaaattggtg
     2281 aagatttata cgaagttacc ttacacatta cggtgcaaac tacgttagaa gacagcaatg
     2341 atgtcgcttt catttgtgaa gtaaaacaag caggtgtgtt tactattaaa ggtttagaag
     2401 gcattcaact tgctcactgc ttagcttcac aatgtccgaa tgtgctttat ccttatgctc
     2461 gtgagctaat ctcaagcctt gtgaatcgtg gtacattccc ggcattaaat ctctcaccgg
     2521 taaactttga tgcgttattt atggattatc tccaacgcca agaagaaagt gcagaagagc
     2581 cggctttagc gaactaagca ttttagatag aaaataggta tgcaaggcat acctattttt
     2641 ttacaagcgg ttattttttc tactattttt tgcaattttc taaataagcc gaacagagga
     2701 aatatgagtg aaatctattc tgccccaatc acggtattag gtgcaggttc ttatggaaca
     2761 gctttagcca ttgccctttc tcgaaatggt cacaaaacct atttatgggg gcataatccc
     2821 gacaaaatgg ccgttttggc atcagagcga atgaaccaag attttctgcc tgatattgta
     2881 ttcccggaag ccttagaaat tgtcgcagat cttgctacag cggtacaaaa atcgaaagat
     2941 ctgctgattg tggtgccaag tcatgtattc tctgaagtat tggctcaaat caagcctttt
     3001 ttgtcccaag atcaccgtat aatgtgggca accaaagggc tggagcgaga taccgggcgt
     3061 ttgttgcaaa cggtagctaa ggaaattttg ggggaaattt acccgcttgc ggtgctttcc
     3121 ggtccgactt ttgccaaaga actggcggca ggcttgccaa cagcgatttc gcttgcctca
     3181 actgatcctg tttttgccga tcaaatgcaa aaacggattc actgttccaa agcgttccga
     3241 gtttacctca acaatgatat tatcggtgtg caactcggtg gggctattaa aaatgtgatt
     3301 gcaattggtg ccggtatttc agacggaatg ggattcgggg cgaatgctcg taccgccttg
     3361 attactcgag ggctggcgga gattagccgt ttgggtgttt cgctcggggc tgatccgaaa
     3421 acctttaccg gaatggcggg gcttggagat ttagtgctga cgtgtaccga taaccaatcc
     3481 cgaaaccgcc gatttggttt ggcgttaggg caaggaaaat cggcagaagt cgcgatagcg
     3541 gaaatcggtc aagtggtaga aggattttat aataccaaag aagcctattt gctcgctcaa
     3601 acacagggcg ttgaaatgcc gattgtagaa caaatttacc aaatgttatt ttgtggcaaa
     3661 caggcttcgg atgtggtgaa aaccttgttg gggcgtgaac gaaaaggcga ataaagaaag
     3721 gagaataaaa tgcagcagag tgaaattaat cagatttggc aagcaattcg agatgaagcc
     3781 aaagagctgg cgaattgtga acctatgtta gcaagttttt tccacgctac cattttaaaa
     3841 caccataatc taggcgatgc gttaagttat attttggcga ataagttaga aaacccgatt
     3901 atgccggcaa tcgccttaaa agaaattatt gaggaagctt atcgagcaga gccacaaatt
     3961 attgctagtg cagcttgtga tattaatgcg gtcagaacta gagatcctgc ggtggacaaa
     4021 tggagtacac ctctgctgta cttaaaaggc ttccacgctt tacaaagcta tcgggtgacg
     4081 cattatcttt ggaatcaagg caggaaagca ttagcggttt atttacaaaa tgaaatttct
     4141 gttgcctttg atgtggatat tcatcctgcg gcaaaggtcg gttgcggaat tatgtttgac
     4201 cacgccaccg gtattgtggt gggtgaaacc tctgtgatcg aaaatgatgt ctcgatttta
     4261 caaggtgtaa cactaggcgg tacagggaaa gaaaatggcg accgccaccc gaaaatccgt
     4321 gaaggcgtga tgattggagc cggtgcaaaa attctcggta atattgaaat cggacgctat
     4381 tctaaaatcg gggcaaattc agtggtgcta caacctgtgc ctgatcacgc cacagcagca
     4441 ggtgtaccgg ctcgcattat cggcaaatcc tctgaacaaa aaccggcttt tgatatgaat
     4501 caatactttg aagatgagca aggtttgttt ggtgatggga tttaggtaag agttattcct
     4561 ttaatttatg atttagacat tgctaagtac aagttgcaat taacctttat tcaaatgttt
     4621 tgcccgctct taagcgggct tactattttc actatttgct atgtatttcc tatcgttgta
     4681 attgaatgct tattttgtca tcaattaatg ctagaattcg ccaaattcta ttttttggat
     4741 gataaatata tgaaaccagc ctcttttaaa ctctctttaa tttctgcatt cgtagggctg
     4801 tcattggcac agcttagtta tagttctacg gttcgtggtg atatagatta ccaatatttc
     4861 cgagatcttg cggaaaataa aggaaaattt tatgtcggag cgaaaaatgt tgaaatattt
     4921 aataagcaag gtgacaagat aggtattgct ttagaaaata ttccaatgat tgacttctct
     4981 gtgattacac gaggaggagg ttatgcaacc ctgactaaac cacaatatat tgtgagtgta
     5041 gagcacaaca gaggctatcg aacagtgcag tttggtgatt ctggtagtga aaacccagat
     5101 aatcaccatt ttaattatca attggtttca cgcaatgatt atgatccaaa agggagtaaa
     5161 gccattcagg gatttacgca taatgactat caaacgcctc gtttgcataa actagtaaca
     5221 gaaacagcac cagcagagca atcccaagag aaaaattatg atctgtatcg tgatgcaagt
     5281 atttttccaa tgtttattcg tgcaggttca ggtacacaat acattttagc tgaaaagaat
     5341 gctgtaaagg aaaaacttgc ggatccgtat gcttacctaa ttggtggaac cacattaaag
     5401 ttaacgagca ataatgctaa tacctttgtt tttaaaggca atgtatttga tgatagttac
     5461 agccctctta gctctcatcc acagcaaggt gatagtggaa agactttcac ttggactgca
     5521 cttggaaata gtagcaggat tacgggggcc acggctgaat tagtagttga tttagctgat
     5581 acctcattgc ctgacccaac aacaggtaag ccacataagc catctttaca ccatgggaaa
     5641 acgcttaaaa tttccggaga tggcaatacc cttaatttag ctaataatat taatcaggga
     5701 gccggagcgc tatatttttc aggtaatgcg gttgtaaccg gtgctaatga aatgacaacg
     5761 tggcttgggg ctgggatatc tgtagataaa aataagaatg tagaatggca agttcataat
     5821 ccggtgggag atagattatc taaaattgga gaaggaacgc taactgtttc tggtaaaggc
     5881 aagaatttag gatcaattag tgttggagat ggcacagtga ttcttaacca acaagcgggc
     5941 gaaaatggtg aaaaatcggc atttagtgaa gttgggatcg ttagtggacg accaacagta
     6001 atcttaaata gcgctgatca agtagatcct aacagtattt attttggtta tcgtggcggt
     6061 cgtttagatt taaacggtaa cagcttaaca tttaatcgga tccagaatgt cgatgatgga
     6121 gcgagaattg ttaataataa tgcaggtact gctgcgaata tttccttagt aggccaagta
     6181 tttacagcta atgatgttcg tacaattaat tttggagaag gctataatgc tgatctgttt
     6241 cgtagccaag gcgcctattt tgtgcttgag aacaatgcgt ggaagtttat aagttggaat
     6301 catgatgatg ctaaaaagta tgtcgttgaa aagaaaaata aagcactaga aaatcaactt
     6361 tatgcttaca atggctattt tggcgaatct gactcaagca gagagaacgg aaagttaaac
     6421 attcacttca agcctattaa tgcagggggg aagctccttt taactggagg taccacacta
     6481 gatgggattt tgtcagcaga gaatgatgct gaaattattt tatccggtcg cccgattcca
     6541 tatgcacgcc aagttcttga tgaaaataaa aaaacaatta ataaggaaat tataaaagaa
     6601 ggtgaatgga ttaatcgaac ctttagcgca acaacctttc aagctaagga taatgggaaa
     6661 attgaagtta gccgtaatgt agttgaagta aacggtaatt ttaatttgag tgacaatgca
     6721 acagctcaag ttggtttcac acaggggaaa tctcaatcat gtgttcgtta ggatcgtacc
     6781 ggcatagcaa gttgtaattt atctgtgtta tctgataagg atttacatag ttggcaacgc
     6841 actacagtca atagtgatat taaattaact cagaatgcac gttttgaatt aggtagtaag
     6901 gctgatctta ttgggacaat tgaaagcaat ggcgatagcc aaataaactt gcgaaatggt
     6961 tctagctggg taatgacagg aaacagcaat gtcaataaat tgaatgtaga taatgcgaca
     7021 atcaccttag acaataatgt gggtgagcca aatactttaa atattaatag ccttagcgga
     7081 agtggggtta ttaactttat cacttacttt gctcaaacaa taagtgattt gataaatgta
     7141 gagcatgcaa gcggtgcttt taaggcaaaa atttcccaaa ttggcacacc cactacgcaa
     7201 aggggcgttt tatgatcttc tataaataca ttaatttgaa gaaggctagc cacctcaatt
     7261 ttaaggtggc taaatatatt gatgaacaag ggaaatcaag aaagatgtat ttaatgaccc
     7321 aagacggctt tacgctttta gtgatgggct ttacccacaa atgaaccgca cttttgccct
     7381 aaataaaatc gccgttgagc ggtgtaagca ggatttcccc gactgctacc actttaaaaa
     7441 gctgatggcg gaggaatgca ccgaactcgc cgaccgtttg aaaatggggc ttgtactgat
     7501 ggcggaatta aaaacaacaa gcaccctaac aagcgaccaa aaccgccatt ttaaagcgtt
     7561 cagaaatggg gctaagtatt taatcaaaca gcttgaataa acggctatac acgttgccaa
     7621 cgtggacaag ggcgaatttg agccgacacc atataacccg attaaacaag cggagggaaa
     7681 tccgcccaca cttggagcag tttaaaacgt tgctctaggc gttcgcctct atcataggcg
     7741 gctttgattc ggtcgctgtt taaatgcgat aaggcaacct cgattaaatc gctattgtag
     7801 cctttctcat taagataagt ggaggcgatg gcccgcaagc cgtgagccac taatttgccc
     7861 ttgtagccca ttcgtttgat cgcaccgttg gcggtttgag tattgacgtg cgtttgtggg
     7921 ttcttcacgc tagggaataa atacgtttta ccgccactta gctttttaat ctctcgcaaa
     7981 agtgcgattg cttggcgtga gagtgtgatt ttatgctcaa tgcctcgctt catattttcc
     8041 gccggtatcg tccacagttt ggctttttcg tctatatccg cccattttgc cttagccgct
     8101 tcattcgggc gggtcattgt gagtaactgc cacagaatta aatagcgtgt ctgtaaatga
     8161 atttgggcgt tttgtaaggt gtataaaaac tcgcctaaat cttccggctt aatggttttg
     8221 aaatgttcaa ccgttgggct atcaaactct ttcgcaatat tggcggtagg gttaaacggt
     8281 atcatttcac ggtgtacggc aaacatcata atttcattat gtttttgaat tgcccgtttt
     8341 aacttctcta gtttgccctc gtcttggtat tgcttgaatg ccttgattgc cattggtgcg
     8401 gttatttgtg tgataggcat tcttccaaag tgcggcaaga ggttattttt aattaaggcg
     8461 attacatctt tttggtagtt gtctgaaaaa ttcttcttgg tcttgcggtg ttccagccac
     8521 gcccacgcta cattttcata agtgttgctg atttcgttgg cctttgcctg ttcctgttgt
     8581 tggcggtggg tttgagggtc gatattttga gccaataatg ccctatattc ttctctaatt
     8641 tctctcgctt gtgctaaaga aatatcaggg taagccccaa tgcttaaatc attccgcttt
     8701 tttgtgaacg gtctttcata cctaaaccgc cacaatttag aatttgaggt aataacaagt
     8761 agtaagccgt taccgtctct taatgggctg tcttccggtt tcgcttttct tatttgggta
     8821 tcgtttaacg gtgtgatttt tctagccata gtaaaccttt aggagtataa agcctaaatc
     8881 ttaggagtac gaatttttgg gggtatttcg gcgtactcct aaatatactc ctaaaatctt
     8941 cggatttgat tgtatcgtat aggtacgcaa cggacaataa aaaagccttg aaacattgat
     9001 tttcaaggct ttttaggtat cggttgaacc gtataggatt ataatttggt ggagctgggg
     9061 ggatttgaac ccccgtccga aatttctcta ccttcagtac tacatgttta gtctagtctt
     9121 taatttcgct tattcacagc ggacagacac gcaatgaata aactagcttt atttaattta
     9181 gtgtttcgat cctaaagcgg cggcgtccac acgatctcgt tttgggtgac ttatctttgc
     9241 cctcgtctta cgagcggaag ctgaggagat aaggctttaa agcaggttat taagctgcta
     9301 aagcgtattg ttcgtcgttt gcgactatta ttttgcggtt tgtttacgag gcctaccgca
     9361 cctcgacatg caccttgggc ttcgccaatc ccgtcgaatc caaaatcagc cccaaaagta
     9421 gcggtattct aaaggaaagc ggtcgaattt gcaaaaagtt ttgcaagtgg cggaacttag
     9481 cggtattgaa tagctcgaaa taaaatcata caaatcccac accagataga tttaacgatg
     9541 ttataatgta aaccttgctg agagtgaaga gaaatagaat gaaaaaattt aacgagaacg
     9601 agattgaaga tgctgaaatt atagatgccg agccgattga tgttgatgag atagacgaag
     9661 aagaaacatc ggtagaagca atgcatccgg ctttggtttc cggctcaatg cctgtgccgc
     9721 agggcaattt ggatagctac atccgaatgg caaatcagta tccgattctc accgccgagc
     9781 aagaaaaaga actggcggag cgttattatt atgatgaaga cgttgaggcg gcaaaacagc
     9841 tgatcctttc ccatatgc
//
LOCUS       DANJWC010000086         9801 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.086, whole genome shotgun sequence.
ACCESSION   DANJWC010000086 DANJWC010000000
VERSION     DANJWC010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9801
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.086"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..213
                     /locus_tag="PWX02_001513"
     CDS             <1..213
                     /locus_tag="PWX02_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005558558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HDL1729511.1"
                     /translation="GSKTASRLAFNRGLITQGEVWLEMIKSRNITVHTDDETILTEEF
                     NKIIKAYLPLFIEFEQRVETLCQTLD"
     gene            195..506
                     /locus_tag="PWX02_001514"
     CDS             195..506
                     /locus_tag="PWX02_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL1729512.1"
                     /translation="MPNFGLSERATKTITDILAKYPEVEQAIIYGSRAKGNYREGSDV
                     DLTFKGKDLTYDIVKRIWGDLDDSDSPYLFDLSIYHQLTNANFIEHIDRVGKVFYQRE
                     L"
     gene            617..1780
                     /gene="mnmA"
                     /locus_tag="PWX02_001515"
     CDS             617..1780
                     /gene="mnmA"
                     /locus_tag="PWX02_001515"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDL1729513.1"
                     /translation="MTTQLTSKTYDTHFPKLTPEQLAENSKKKVIIGMSGGVDSSVSA
                     FILQQQGYQVEGLFMKNWEEDDDTDYCTAAADLADAQAVADKLGMKLHKINFAAEYWD
                     NVFEYFLAEYKAGRTPNPDILCNKEIKFKAFLEYAAEDLGADYIATGHYVRRTSDDQD
                     AKLLRGLDENKDQSYFLYALNKKQIGQSLFPVGEIEKPIVRVIAEDLGLATAKKKDST
                     GICFIGERKFKDFLARFLPAQPGEIRTVEGKLVGRHDGLMYHTLGQRKGLGIGGVKGL
                     SEDPFYVVEKDLINNVLIVAQGHDNSALLSSGLIAKQLTWVDMQPLRENLRCTVKTRY
                     RQADIACEIQVLDDETIRVMFDEPQIAVTPGQSAVFYLGEVCLGGGIIESQLK"
     gene            1897..3276
                     /gene="argH"
                     /locus_tag="PWX02_001516"
     CDS             1897..3276
                     /gene="argH"
                     /locus_tag="PWX02_001516"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDL1729514.1"
                     /translation="MALWGGRFTQQADAKFKYFNNSLRFDYRLAIQDIEGSIGWAKAI
                     TSVGILTVAELEQLIAALKEVRAEVESNLTIILKDDAEDIHSWVESKLIEKVGDLGKK
                     LHTGRSRNDQVAVDIKMWCKVQAVALQERIRALQERLVSVAEENQESVMPGYTHLQRA
                     QPVTFAHWCMAYYEMLERDFSRLSDANKRMSTCPLGSGALAGTAYGIDRDLLARDLGF
                     EEATRNSLDSVSDRDHILELLSTASISMMHLSRFAEDLIIFNSGESGFLEMSDRVTSG
                     SSLMPQKKNPDACELIRGKSGRVFGALNGLLTTLKGLPLAYNKDMQEDKEGIFDALET
                     WQACLEIAELVLVDIQVNTERTREAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VYAISKKEPLEALSINEFQQFHHSIGDDVYPILSLESCLEKRCAKGGVNPQRVAEAIA
                     VAKANLASK"
     gene            3444..4442
                     /locus_tag="PWX02_001517"
     CDS             3444..4442
                     /locus_tag="PWX02_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HDL1729515.1"
                     /translation="MTESVNSTQAGTALSLGQQLKAAREALNLSITDVAQKTNLKKSH
                     IESLENDIFILQNVAPTFVRGYVRNYVKFLRLPESLISSVNYGEVTIPKEVTKVSSVK
                     PSSNSQGKTLKYLTVLVLLAALGMTLLWWWQGYQKEQENREQFVSSAPVAEVANTATN
                     NDASAQTSVPVQQPIQVSEQPAQVAETVVAAEPALTEKVEPTQAASSPVTQEDVKVVE
                     EVKQADATTAQVEAVNVLQQSQSEQAPTEQPVAVTNDELRIEITGSQSWITVRGAKNK
                     RLAEKLYNNGETLSFNENEQYRLTIGAPANVKVYYKGQEVPLKIDGRVARIRLPLQ"
     gene            4545..5657
                     /gene="ispG"
                     /locus_tag="PWX02_001518"
                     /gene_synonym="gcpE"
     CDS             4545..5657
                     /gene="ispG"
                     /locus_tag="PWX02_001518"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDL1729516.1"
                     /translation="MIHQSPIKRRVSKKIYVGNVPVGGDAPITVQSMTNTRTTDVEAT
                     VAQIKSLERVGADIVRVSVPTMDAAEAFKAIKQQVSVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRTVVDCARDKNIPIRIGVNAGSLERDLQEKYGEPTPQALLES
                     ALRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARAGSV
                     KSAVGLGLLLAEGIGDTLRVSLAADPVEEVKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EIDVIATVNELEQRLEDIITPMDVSIIGCVVNGPGEALVSDLGVTGSNKMSGFYLDGV
                     RQKERFDNAKLIDQLEAKIRARVAQQHNRIDIEQIG"
     gene            5663..6940
                     /gene="hisS"
                     /locus_tag="PWX02_001519"
     CDS             5663..6940
                     /gene="hisS"
                     /locus_tag="PWX02_001519"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDL1729517.1"
                     /translation="MAKTIQAIRGMNDCSPSESPLWQWVEQKVRNVLASYGYSEVRMP
                     IVESTPLFARAIGEVTDVVSKEMYTFWDNDEQLTLRPEGTAGCVRAAIERGWIYNNEQ
                     RLWYMGPMFRHERPQKGRYRQFHQAGVEVFGIANPEIDAELIILTARLWKELGIDQHV
                     SLQLNSIGSLEARANYRSALVKFLENHTALMNEEEKERLVKNPLRILDTKNEALQNVL
                     NDAPKLLDYLDDDSREHFTQLCALLDAMGIRYEVNPKLVRGLDYYNKTVFEWVTSALA
                     LGSQGTVCGGGRYDGLVEQLGGHATQGVGFAMGLERLVLLVQEVNKEIDLPKTVDVYI
                     VFSGEGATLNAFQLAEQIRSELPHVGVMTHCSGGKFQKQFKRADKVEAQIALVIGESE
                     VAEKQVVIKDLRSGAEQITIAQSELIAELSKRF"
     gene            6950..7570
                     /locus_tag="PWX02_001520"
     CDS             6950..7570
                     /locus_tag="PWX02_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL1729518.1"
                     /translation="MSNEYLNKTEEQQFEDAKNWFKENGTPILVAIILACAATAGWNF
                     WKNHQLETAQKTSASYQSVMESYLQNPSKNQPLVEKFIADNKDSSYAAFAQFEVAKQA
                     VEKGNFSAAKTALQSVLTYSDSTLQSIARFRLAQIDFQLKSYDEALASLAQIQDKAWE
                     LRKQILTGDILAAKGDIPAAKSAYEQAKTTANPQEQTLIDVRLNNL"
     gene            complement(7633..8736)
                     /gene="dnaN"
                     /locus_tag="PWX02_001521"
     CDS             complement(7633..8736)
                     /gene="dnaN"
                     /locus_tag="PWX02_001521"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDL1729519.1"
                     /translation="MQITITREQLLKPLQQVCGVLSSRPTLPVINNLLLEIEGNRLSL
                     TGTDLEVELTTQAQLLQAVDFAGKFTIPAKKFLDISKSLPDDSVISVQFEEDKAIIKA
                     GRSKFSLATLPASDYPNLMDWKPEVDFSIDQSTLARLIDATQFSMANQDARYFLNGMK
                     FETEGNLLRTVATDGHRLAVCTMVLNQELLTHSVIVPRKAVLELSRLVASNDETVRLE
                     IGSSNIRITMNGIVFTSKLIDGRFPDYRRVLPRNADRILEAETETLKRALMRAAILSN
                     EKFRGVRLTLSNNLLKITANNPEQEEAEEIIDVAYDSPEMEVGFNVSYVLDVLNTLKC
                     QRVRFSLVDSSSSCLIEDCDNSSAEYVIMPMRL"
     gene            complement(8755..>9801)
                     /gene="dnaA"
                     /locus_tag="PWX02_001522"
     CDS             complement(8755..>9801)
                     /gene="dnaA"
                     /locus_tag="PWX02_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDL1729520.1"
                     /translation="FSNTGFKTGLNPNQTFDNFVQGRSNQLAKLVAQQVVDNLGESHC
                     NPLSFYAGTGLGKTHLLHAIGNELVKRTPGTRVLYIHLERFYRDIIKAINSSNNDAEK
                     IKKFYRSLDVLMIDDIQFLANKPKVQEEFLHLLNSLFEQRKQIILTSDVLPKNIDKID
                     TAIKSRLSGGISATIEPPELETRVAILRQKALERGADLQEDAAYLMGQKLRTNVRELE
                     GALNRVIAWQKLTLRPITVDAVRETLQDLFASFDYLITIENIQRIVAEYYGIKVADIK
                     SKSRARSIARPRQMAMALAKELTNHSLPEIGREFGGRDHTTVMHACKTINELRDTESG
                     IQEDYIHLTRKLSS"
BASE COUNT         2920 a         1892 c         2236 g         2753 t
ORIGIN      
        1 ggttcaaaaa cggcttctcg tttggctttt aatcgtgggc taatcactca gggcgaagtt
       61 tggctagaaa tgataaaaag ccgcaatata acagttcata ctgacgatga aactatttta
      121 actgaagaat ttaataaaat tatcaaggcg tatctgcctt tatttattga atttgaacag
      181 cgagtagaaa cactatgcca aactttggac taagcgaacg agcgacgaaa actattaccg
      241 atattcttgc gaaataccct gaagtggagc aagccattat ttacggctcg cgtgctaaag
      301 gtaattatcg tgaaggctca gatgtagatc ttacttttaa aggaaaagat cttacttacg
      361 atattgtcaa acgcatttgg ggtgatttgg acgattccga ctcgccctat ttatttgact
      421 tgtcgattta tcatcaatta accaatgcta actttattga acatattgat agagtaggaa
      481 aagtctttta ccaaagagaa ctctaatttc ttaataggca agcggttcat tttgcaaaat
      541 tttttgcaaa tttcaccgct tgttgttttt attattaacc aatgtgcttc gccttaacca
      601 aagttacggg agatttatga caactcaatt aacctcaaaa acctacgaca ctcatttccc
      661 taaattaacc ccagaacaac ttgctgaaaa cagcaagaaa aaagtcatta tcggtatgtc
      721 cggtggggtg gattcctcgg tttccgcttt tattcttcaa cagcagggct accaagtgga
      781 aggcttgttt atgaaaaact gggaagaaga tgatgatacg gattattgta ccgccgccgc
      841 cgatcttgcc gatgctcaag cggttgccga taaactgggt atgaaactgc ataaaattaa
      901 ttttgcagcg gaatattggg ataatgtatt tgaatatttc ttggcagaat acaaggcagg
      961 aagaacgcca aatccggata ttttgtgtaa taaagagatc aaatttaaag cctttttgga
     1021 atatgcggca gaagatcttg gtgcggatta tattgcgacc gggcattacg tccgccgtac
     1081 gagcgatgat caagatgcga aacttttgcg tggcttagat gaaaacaaag atcagagtta
     1141 ctttttatat gcgttaaata aaaagcagat cgggcagagt ttattcccag tgggtgaaat
     1201 tgagaagccg attgtgcgag tcattgccga agacctagga ttagcaaccg caaagaaaaa
     1261 agattctacc ggcatttgtt ttatcggtga gcgtaaattt aaagatttct tagctcgttt
     1321 tctaccggct caaccggggg aaatccgcac cgttgagggc aaattagtcg gtcgccatga
     1381 tgggctgatg tatcacacgc ttgggcaacg caaaggcttg ggtatcggtg gggtgaaagg
     1441 gctaagtgaa gatccgtttt atgtggtgga gaaagactta attaacaatg tattgattgt
     1501 ggcacaagga cacgataatt cagccctgct ttcaagtggc ttaattgcga aacagctcac
     1561 ttgggtggat atgcagccgt tgcgtgaaaa tttacgctgc acggtaaaaa cccgttatcg
     1621 tcaagcagat attgcgtgtg aaatccaagt gcttgatgat gaaaccattc gagtgatgtt
     1681 tgatgaaccg caaattgccg tgacgccggg gcagtctgcc gtgttttact taggtgaagt
     1741 ttgcttaggc ggtggcatta ttgagtcaca attaaaatag ttataaataa atattgaaat
     1801 attttgcata tttatgcaaa ataagcgaaa agttaaggcg gtaaaacgcc tttttcattt
     1861 tgatcgatac aaagaataat atacaaggaa cagactatgg cactttgggg tggacgcttt
     1921 acacaacaag cagacgcaaa atttaaatat tttaacaatt cattacgttt tgattaccgc
     1981 ttggcgattc aagatattga aggctcaatc ggctgggcaa aagccattac ttcggtaggt
     2041 attttaacgg tagcagaatt agaacaacta atcgcagcat taaaagaggt gagagcagag
     2101 gtagaatcta accttaccat catcttaaaa gatgatgcgg aagacattca cagctgggtt
     2161 gaatctaagc tgattgaaaa agtgggcgat ttaggtaaaa aattgcacac cgggcgtagc
     2221 cgtaatgacc aagtagcggt ggatattaaa atgtggtgca aggtgcaagc ggtggcattg
     2281 caagaaagaa tccgagcttt gcaagagcgt ttggtttccg ttgccgaaga aaatcaagag
     2341 tcggtgatgc cgggctatac ccatttacaa cgagctcagc ctgtgacctt cgctcattgg
     2401 tgtatggcgt attatgaaat gctagaacgg gatttcagcc gtttgagtga tgctaataaa
     2461 cgtatgagca cttgcccgct tggttcaggg gcattggctg gtacggctta cggcattgat
     2521 cgtgacttat tagcccgtga tttaggcttt gaagaagcca cacgcaacag tttagacagc
     2581 gtgtcagatc gtgaccatat tttagagttg ctctctactg cctcgatcag tatgatgcac
     2641 ctctctcgct ttgctgaaga tttaatcatt tttaatagtg gcgaatcagg cttccttgaa
     2701 atgtcggatc gtgtgacctc aggctcatca cttatgccac agaagaaaaa ccctgatgct
     2761 tgtgagctga ttcgtgggaa atccggtcgg gtatttgggg cattaaacgg tttattaact
     2821 acactcaaag ggttaccact tgcttataat aaagatatgc aagaggacaa agagggtatt
     2881 tttgatgcgt tggaaacttg gcaggcttgt ttagaaattg cagaacttgt actggttgat
     2941 attcaagtga ataccgaacg cacccgagaa gccgctcagc aaggttatgc gaatgcgacc
     3001 gagttggcag attatttagt ggcgaaaggc gtgccattcc gtgaagctca tcatattgtt
     3061 ggcgaagcgg tggtttacgc aatcagcaaa aaagagccac ttgaagcatt aagcattaac
     3121 gaattccagc aattccatca ttcgattggc gatgatgttt acccaattct ctcgttagaa
     3181 tcttgcttag aaaaacgttg tgccaaaggt ggagtgaacc cacaacgtgt ggcagaggcg
     3241 attgcggtag ccaaagccaa tttagcttca aagtaaaacc ggatacaagc ggtcatattt
     3301 tgcaaaaaaa ttgaaaaata tgaccgcttg ccgattaaat tttcaatttt ttacgcatta
     3361 agattgcaaa ataggcatta cctctttaaa cttccgaata atttttgaat tttatttcta
     3421 ctactttctt taaagagttt tatatgacgg aatcagttaa ttctactcaa gctggtacag
     3481 cactttcttt agggcaacaa ttaaaagcgg ctcgtgaagc gttaaatctc tctattacag
     3541 atgttgctca aaaaaccaac ttaaaaaaat cacatattga atcacttgaa aatgatatct
     3601 ttattctaca aaatgttgcc ccaacttttg tgcgtggcta tgtgcgtaat tatgtgaaat
     3661 ttttgcgttt acctgaatcc cttatttcga gcgttaatta tggtgaagtc actattccaa
     3721 aagaagtcac taaagtttct tcagtaaaac cttcatcaaa ttcacaaggt aaaaccttaa
     3781 agtatttaac ggtattagta ttgcttgcgg cattaggaat gacattactt tggtggtggc
     3841 aagggtatca aaaagaacag gaaaatcgcg agcaatttgt tagctctgca ccagttgctg
     3901 aagtggcgaa taccgctaca aataatgatg cttcagcaca aacttccgtg cctgttcagc
     3961 aacctattca ggtttcagag cagcctgctc aagtagcaga aacggtagtt gcagcagaac
     4021 cggcattaac tgaaaaagta gagcctacac aagcagcatc atcacctgta acacaagaag
     4081 atgttaaagt ggtagaagaa gttaaacaag ctgatgccac cactgcacag gttgaggcag
     4141 ttaatgtttt acagcaatct caatcggaac aagcccctac agaacagcct gttgcggtaa
     4201 cgaatgatga gcttcgtatc gaaattaccg gttcacaaag ctggattaca gtgcggggtg
     4261 ctaaaaataa acgtttagca gaaaaattat ataacaatgg tgagacttta agtttcaatg
     4321 agaatgagca atatcgttta accattggtg cgcctgccaa tgtaaaagtg tactataaag
     4381 gtcaagaagt accattgaaa attgatgggc gtgtagctcg tattcgttta ccgttacagt
     4441 aagcttgatt taacgatgaa aaacgcacgt aaagctagca tttaacgata gctgtcttta
     4501 cgagcgtttt ctgtttttat aaattttcac aattagctaa aaatatgata catcaatcac
     4561 ctattaaacg tcgggtatcg aagaaaatct atgtgggtaa tgtgcctgta ggcggcgatg
     4621 caccgattac ggttcagtca atgaccaaca cccgcacaac cgatgtggaa gccaccgttg
     4681 ctcaaattaa atcgctggaa cgggtcgggg ctgatattgt gcgtgtgtct gtgccgacaa
     4741 tggacgctgc tgaggcattt aaagcgatta aacaacaagt gagtgtgcca ttggtggcgg
     4801 atatccactt cgattaccgc attgcgttga aagttgccga atatggggtg gattgcttgc
     4861 ggattaaccc gggtaatatc gggaatgaag agcggatccg cacggtggta gattgtgcca
     4921 gagataaaaa cattccgatc cgaatcgggg taaatgccgg atcattagag cgtgatttgc
     4981 aggaaaaata tggcgagccg acaccacagg cattattaga atcggcatta cgccacgttg
     5041 atcacttaga tcgccttaac tttgatcaat tcaaagtcag cgtgaaagcc tcagatgtat
     5101 ttctagcggt tgaatcttac cgacttcttg caaaacagat tgatcagccc ctgcatttag
     5161 gtattactga agcaggcgga gcgagagccg gttcggtgaa atcggcggta ggcttaggtc
     5221 tgttgctagc ggaagggatt ggtgatacct tgcgtgtgtc attagcggct gatcctgttg
     5281 aagaggtgaa agtcggtttt gatattttga aatcgctgcg tattcgctct cgtgggatca
     5341 actttattgc ttgcccaacc tgctcccgcc aagaaattga tgtgattgcg acggtaaacg
     5401 agctagaaca acgtttagaa gacattatca cgccgatgga cgtttcgatt atcggctgtg
     5461 tggtaaacgg tccaggagag gcgttggtgt ctgatttagg ggtaaccggc agcaataaaa
     5521 tgagcggctt ttatttagat ggcgttcgtc aaaaagagcg ttttgacaac gcaaaattaa
     5581 tcgaccaatt agaagctaaa attcgtgcca gagttgcaca acaacataat cgtatagata
     5641 ttgaacaaat aggataaata tagtggcaaa aacaattcaa gcaattcgag gaatgaacga
     5701 ctgctcacca agtgagtctc cattatggca atgggttgaa cagaaagtgc gtaatgtgct
     5761 ggcaagttac ggctattcgg aagtacgtat gccgattgta gaaagcactc cgttatttgc
     5821 ccgtgccatt ggcgaagtaa ccgatgtggt gtcgaaagag atgtacacgt tctgggataa
     5881 cgatgagcaa ttaacgctcc gcccggaagg tacagccggt tgtgtgcgtg cagcgattga
     5941 gcgtggttgg atctacaata atgagcaacg cttatggtat atggggccga tgttccgcca
     6001 cgaacgtcca cagaaagggc gttaccgcca attccaccaa gccggtgttg aagtgtttgg
     6061 cattgcaaat ccggaaattg atgcggagtt gattattctc accgcacgcc tatggaaaga
     6121 gttaggcatc gatcaacatg tttctttgca acttaactct atcggctcgt tagaagctcg
     6181 tgcaaattat cgctcagcat tagtaaaatt tttagaaaat cacaccgctt taatgaacga
     6241 agaagagaaa gagcgtttgg tgaaaaaccc gctacggatt ttagatacca aaaacgaagc
     6301 actacaaaat gtattaaacg atgccccaaa actgctcgat tatttagatg atgacagccg
     6361 tgaacatttt acccaacttt gtgccttact tgatgcaatg ggaatccgtt acgaagtcaa
     6421 tccaaaattg gtgcgtggtt tagactatta caataaaacc gtgtttgaat gggtcacttc
     6481 agccttagcc ttaggctcac aaggcaccgt atgtggcggt gggcgttatg acggcttagt
     6541 cgaacaactc ggtggacacg caacccaagg cgttggcttt gcaatgggct tagagcgttt
     6601 agtgctgttg gtacaagagg tgaataaaga gattgattta ccaaaaacgg tggatgtgta
     6661 tatcgtattc tccggtgaag gggcaacact aaatgccttc caacttgcag agcagattcg
     6721 tagcgaattg ccacacgttg gcgtaatgac acactgttcc ggcggtaaat tccaaaaaca
     6781 atttaagcgt gcagataaag tagaagcgca aattgcgtta gtgattggcg aaagcgaagt
     6841 ggctgaaaag caagttgtta tcaaagattt acgttcagga gcagaacaaa tcacgattgc
     6901 tcaatcagag ctgattgctg agctatctaa acgattttag gagaaaaaga tgagtaacga
     6961 atatttaaat aaaaccgaag agcagcaatt tgaagatgcc aaaaattggt ttaaagagaa
     7021 cgggacaccg attttggtgg cgattatttt agcttgtgcg gcaactgccg gctggaattt
     7081 ttggaaaaat caccagcttg aaacggcaca aaaaacatcc gcttcatacc aaagcgtgat
     7141 ggagagttat ttgcaaaatc caagcaaaaa tcaaccgctt gttgagaaat ttattgccga
     7201 taataaagat tcaagctatg ccgcttttgc ccaatttgaa gtagctaaac aagcggtcga
     7261 aaaagggaat ttttctgcgg caaaaacagc gttacagtca gttttgactt attcggattc
     7321 aaccttacaa tctatcgctc gttttcgttt ggctcaaatt gacttccaac taaaatctta
     7381 cgatgaagca ctggcaagtc tggctcaaat tcaagataaa gcgtgggaat tgcgtaagca
     7441 aattctaaca ggcgatattc ttgcagcaaa aggcgatatt cctgcggcga aaagtgctta
     7501 tgagcaagct aaaacgaccg caaatcctca ggaacaaacc ttaattgatg tacgtttgaa
     7561 taacttataa aaacaaagcc tgattgaaat aaaatcaatc aggctttttt gttggtttag
     7621 tgttgctgtg aattataaac gcattggcat aatcacatat tcggcagagc tgttgtcgca
     7681 atcttcaatt aagcaactag aacttgaatc aactaagcta aaacgcacac gttggcattt
     7741 taaggtgttt aacacatcta atacatagct aacgttaaag ccgacttcca tttccgggct
     7801 gtcgtaggca acatcaatga tctcttccgc ctcttcttgc tccggattgt ttgcggtaat
     7861 tttgagtaaa ttattgctta aggttaaacg cacccctcta aatttctcat tagaaagaat
     7921 cgctgcacgc attaatgccc gtttaagggt ttcggtttcc gcttctagaa tgcgatctgc
     7981 attgcgaggc agaactcggc ggtaatccgg gaatctgcca tcaatcagtt tagaggtaaa
     8041 tacaatgccg ttcattgtaa tacggatatt gcttgaaccg atttccaaac gcacggtttc
     8101 atcgttgctg gcaaccaagc gggaaagctc aagtacggct ttgcgtggca cgataaccga
     8161 atgagtcaaa agctcttgat ttagcaccat tgtgcaaacc gctaaacggt gcccatctgt
     8221 ggcaacggtg cgtaataaat tgccttcggt ttcaaatttc attccgttta agaaataacg
     8281 ggcgtcttgg tttgccattg agaattgggt ggcatcaatc agacgggcaa gggtagattg
     8341 atcaatcgag aaatccactt ccggtttcca atccatcaaa ttcgggtaat cacttgccgg
     8401 taatgtagct aaactaaatt tactacgtcc tgctttgata atggctttat cttcttcaaa
     8461 ttgtaccgaa atcacgctgt catcaggtag gcttttactg atatctagga attttttagc
     8521 tggaatggta aattttccgg caaaatcgac cgcttgtagc agttgtgctt gggtggttaa
     8581 ttccacttct aaatcggtac cggttaaaga tagacgatta ccttcaattt ctaataaaag
     8641 attgttaata acaggcaaag tcggacggct acttaatacg ccacacacct gttgtaaagg
     8701 cttaagtaat tgttctctgg taatggtaat ttgcataatc attccttcat cgggctaaga
     8761 tgataatttg cgtgttaaat ggatataatc ttcttggata ccgctttcgg tatcgcgtaa
     8821 ttcattaatg gttttgcagg cgtgcatcac ggtagtatga tcccgaccgc caaattcccg
     8881 accaatttcc ggcaagctgt ggttggttag ctcttttgcc agtgccattg ccatttggcg
     8941 aggacgggca atgcttcttg cccgactttt cgatttaata tcggcaactt ttatgccgta
     9001 atattcggcc acaatgcgtt gaatattttc aatcgtaatc aagtaatcaa aagaggcaaa
     9061 cagatcttgc aaggtttcac gcaccgcatc aaccgtaatc gggcgaaggg tcaatttttg
     9121 ccacgcaatc acacggttta atgctccctc caattcacgc acattggttc ggagtttttg
     9181 ccccattaaa taggcggcat cttcttgcaa atctgctcca cgttctaagg ctttttggcg
     9241 aagaattgcc actcgggttt ccaattcagg tggctcaatg gtcgcactaa taccgccgct
     9301 taaacgggat ttaatcgccg tgtcaatttt atcaatattt ttcggcaaca catcggaggt
     9361 taaaataatc tgtttgcgtt gttcaaacag cgaatttaac agatgtaaaa attcttcttg
     9421 tactttcggt ttattggcaa gaaattgaat atcgtcaatc attaacacat caagcgagcg
     9481 gtagaatttt ttgatttttt ccgcatcgtt attggaactg ttgatcgcct taataatatc
     9541 acgataaaaa cgttccaagt gaatatatag cacccgagta cccggtgtgc gtttaactaa
     9601 ttcattgccg atagcgtgca ataagtgggt tttgcctaaa ccggtgccgg cataaaacga
     9661 gagtgggttg caatggcttt cgcctaaatt atccaccact tgttgagcca ctaatttagc
     9721 taattggttt gagcggcctt gtacaaaatt atcaaaggtt tgatttgggt ttagcccggt
     9781 tttaaagccg gtattgctaa a
//
LOCUS       DANJWC010000087         9662 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.087, whole genome shotgun sequence.
ACCESSION   DANJWC010000087 DANJWC010000000
VERSION     DANJWC010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9662
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.087"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(206..1495)
                     /locus_tag="PWX02_001523"
     CDS             complement(206..1495)
                     /locus_tag="PWX02_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL1729521.1"
                     /translation="MDSIPLSSLFITLAILLVLSAFFSGSETGLMSLNRYKMRHLAEQ
                     GHKGAKLAEKLLARTDVLLSLILICNNLVNIAASAIATMIGMQLSGEAGVAIATGLLT
                     FVMLVFSEILPKTVAAIYPEKVGFFASFVLTPLKKLLSPIVFLMNLIIAGLMKLLRIQ
                     QKDKQGLSSEELRSVVLDAGKFIPAEHREMLVSILDMEKVTVDDIMVPRNDISGIDID
                     DDWKAIMRQLNHAPHARVIIYKGDMDNNIVGILRVREAFRLMLEKNEFTKEMLLRATD
                     EVYFIPEGTPLTTQLMNFKANKERIGLVVDEYGDIKGLVTLEDILEEIVGEFTTSSAP
                     TLEQEVQQQSDGTVIIEGSANLRDLNKLFDWDLPLEEARTFNGLILEHLEKIPDEGME
                     FELYNLKVMILEVSENMVKQAKVEHFSPKIEKEQDSA"
     gene            complement(1619..2416)
                     /gene="ccsA"
                     /locus_tag="PWX02_001524"
     CDS             complement(1619..2416)
                     /gene="ccsA"
                     /locus_tag="PWX02_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HDL1729522.1"
                     /translation="MLLPLLSIVAYALALLWVAPTLVNLENAATVNKKPNMMLVFGFG
                     LLATILHFSVLYNELFLIEGQNFTVTNVISLMSFIMAACVTLALLKWKTVWFPLIIVY
                     SFAICSVGVAGFVKGSFIRNVQENAGLLFHLGIALFSYALFFLALIYAFQLKWLDRKL
                     KSKTMFFCQILPPLMTVERHFFTLTLAAQAMLTLTLFSGVVYLHNFFEPEHIHKAIFS
                     FLAWLVYSIQLLGQWKLHWRGKRVLIYSISGMMLLTIGYFGSRLIVN"
     gene            2632..3156
                     /gene="yceD"
                     /locus_tag="PWX02_001525"
     CDS             2632..3156
                     /gene="yceD"
                     /locus_tag="PWX02_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005621677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HDL1729523.1"
                     /translation="MQKVKLPLTIDPYKDAQRRVDYEGYISGKLLTRLGESVCNVQSD
                     AQVTLSLYVDPQRLTVIKGTAKVDVEFDCQRCGDPFTQTLDCSFVFSPVSNMDQADIL
                     PEIYEPIEVNEFGEVNLLDMIEDEFILELPLVPMHSEEHCEVSVSEQVFGELPEELVQ
                     KPNPFAVLANLKKN"
     gene            3173..3343
                     /gene="rpmF"
                     /locus_tag="PWX02_001526"
     CDS             3173..3343
                     /gene="rpmF"
                     /locus_tag="PWX02_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006231206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL1729524.1"
                     /translation="MAVQQNKKSRSRRDMRRSHDALTTAAVSVDKTSGETHFRHHVTA
                     DGYYRGRKVINK"
     gene            3362..4384
                     /gene="plsX"
                     /locus_tag="PWX02_001527"
     CDS             3362..4384
                     /gene="plsX"
                     /locus_tag="PWX02_001527"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDL1729525.1"
                     /translation="MVDITLAVDVMGGDFGPRVTIPALEKALKQNPSLSFLLFGDEHQ
                     ITPLLQKHTLLNHPKIQIHHTEQVIEADVPFTTAIRQSKNSSMRLAIEAVKEGKAQGC
                     VSGGNTGALMGLAKLLIEPLPNIERPALTSLIPTLNGKSSVMLDLGANIEVSNHQLQQ
                     FAEMGNLFAQVMLDLVYPRLSLLNIGTEENKGTAQLQAVHQLLKNRQDLNYTGFIESD
                     KLTSYLTDVIICDGYTGNIALKALEGAAKNIISLFKKEKADSNICRNTKRYLLKAMFY
                     RYYRKLQQINPDRHNGATLLGLSKVVVKSHGGANINAFYYAIDYAVQQIQGDIPNKIL
                     EGLTQK"
     gene            complement(4481..5764)
                     /gene="hisD"
                     /locus_tag="PWX02_001528"
     CDS             complement(4481..5764)
                     /gene="hisD"
                     /locus_tag="PWX02_001528"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDL1729526.1"
                     /translation="MQTLIWNNLTETEQQQYLARPAQVVGESIKAAVNEIKANVIANG
                     DKALFELAEKFDKVKLESLVVSEQQIQQAQSRISPELKQAIQNAKANIEAFHKAQQNQ
                     EVDIETQPGVCCQVITRPINAVGLYIPGGSAPLFSTVLMLAVPAKIAGCNKIVLCSPP
                     PIADEILYTAHLCGVETIYAVGGAQAIIAMANGTQTVAKVDKIFGPGNAFVTEAKRQV
                     VQEGTAIDMPAGPSEVLVIADEFADPEFVASDLLSQAEHGADSQVILVTPSESLAKAV
                     AEELEKQVSVLPRAETACKALAHCRTFIAENLQQAVEISNKYAPEHLIVQTEDARSLL
                     ADLDNAGSIFLGAYSPESMGDYASGTNHVLPTYGYTKTYSSLGLADFSKRMTVQELTP
                     QGFLALAETVERMASAELLDAHRNAVSLRVAKLKG"
     gene            complement(5776..6675)
                     /gene="hisG"
                     /locus_tag="PWX02_001529"
     CDS             complement(5776..6675)
                     /gene="hisG"
                     /locus_tag="PWX02_001529"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDL1729527.1"
                     /translation="MTTQNRLRIAMQKKGRLSQECSELLKQCGVKIIWNDQRLIAYSE
                     NMPIEILRVRDDDIPGLVFDGVVDLGIIGENVLEEVELGRLSAGENVAYKKLRQLDFG
                     GCRLSLAIDRDKNYNNIKDLEDAVIATSYPNLLKRYMKEQNVPFKSILLTGSVEVAPS
                     AGIANAICDLVSSGATLEANGLKEVEVIYRSTACLIQRQEPLSAEKQALVDKLLTRIQ
                     GVQQANESKYIMLHAPKDKLAEITALLPGVENPTILPLANDQTRVAMHVVSQENLFWE
                     TMEQLKEMGASSVLVLPIEKMLA"
     misc_feature    complement(6708..6835)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            7034..7891
                     /locus_tag="PWX02_001530"
     CDS             7034..7891
                     /locus_tag="PWX02_001530"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDL1729528.1"
                     /translation="MQISLKKIYSGKVRDLYEIDEKRMLMVATDRLSAFDVILDDPIP
                     RKGEILTQISNFWFNKLAHIMPNHFTGDSVFDVLPQEEAEAIQHRAVVCKRLIPVKIE
                     SIVRGYLTGSGLKDYQQTGTICGLTLPEGLVEASKLPQPIFTPSSKAEVGDHDINISY
                     EECERQIGQELAAKVRDAALKLYTEAANYALTKGIIICDTKFEFGLDENGELTLMDEV
                     LTPDSSRFWSVETYREGTNPPSFDKQFVRDWLENSGWNKQPPAPKIPAEVIEKTVAKY
                     QEALDLLTK"
     gene            complement(8032..9513)
                     /gene="ilvC"
                     /locus_tag="PWX02_001531"
     CDS             complement(8032..9513)
                     /gene="ilvC"
                     /locus_tag="PWX02_001531"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDL1729529.1"
                     /translation="MANYFNTLNLRQKLDQLGRCRFMDRDEFADGCNFLKGKKIVIVG
                     CGAQGLNQGLNMRDSGLDIAYALRAEAIAEKRASFKRASENGFVVGTYEQLIPEADLV
                     INLTPDKQHSKVVADVMPLMKQGAAFGYSHGFNIVEEGEQIRPDLTVVMTAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGLAIAKAWASATGGDRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSIVCYDKLVSEGKDPAYAGKLIQYGWETITEALKQGGITLMMDR
                     LSNSAKLRAFELSEQIKEELAFLFEKHMDDIISGEFSSTMMADWANGDANLLKWREET
                     GKTAFENAPQADGIKISEQEYFDNGVLMVAMVKAGVELAFDTMVSAGIYEESAYYESL
                     HELPLIANTIARKRLYEMNVVISDTAEYGNYLFSHVATPILAEKIIPTLEKGDLGEPT
                     PAVEIDNVTLRDVNDAIRNHPIELIGQELRGYMTDMKRISSQG"
BASE COUNT         2924 a         2168 c         1791 g         2779 t
ORIGIN      
        1 aaacagataa aaaacgctca attttcaata taaaactcca aataaaaaca aaaattcaaa
       61 caaaaaaact aaaaacaaga catcttcagt taaatataaa taaaaaccta aaagctacca
      121 gcataattat ttatattaaa attaacaaaa aaataacaca ttatttgtac aaataatgtg
      181 ttatttactt tcttttttct taaaattagg ctgaatcttg ctctttttca attttcggtg
      241 aaaaatgttc aactttcgct tgtttaacca tattttcact cacttcaaga atcatcactt
      301 tcaagttgta gagttcaaat tccattcctt catcaggaat tttttctaaa tgctctaaaa
      361 tcaagccgtt aaaggttctg gcttcctcaa gcggtaaatc ccaatcaaat agtttgttca
      421 gatcacgcag gttggctgag ccttcaataa tcaccgtacc atctgattgt tgctgaactt
      481 cttgctcaag ggtcggtgct gatgaagtcg taaattcacc cacaatttct tctaaaatat
      541 cttcaagagt gactagccct ttaatatcac catactcatc aaccactaaa ccaatacgct
      601 ctttattcgc tttaaagttc attaattgtg tagtgagtgg tgtgccttca ggaataaaat
      661 agacttcatc tgttgcacgc agtagcattt ctttggtaaa ttcgtttttc tccaacatta
      721 aacgaaaggc ttcacgcacc cgcaaaatcc caacaatatt gttgtccata tcccctttgt
      781 agataatcac ccgagcgtga ggggcgtgat taagctgacg cataatcgct ttccagtcat
      841 catcaatatc aatgccgctg atgtcgttgc gaggcaccat aatgtcatca accgtcactt
      901 tttccatatc taaaatagag accaacattt cacggtgttc ggcaggaata aatttaccgg
      961 catcaagcac tacactacgc agctcttcgg agcttaagcc ttgtttatct ttttgttgaa
     1021 tccgcaataa tttcatcagc ccggcaataa tcaaattcat taaaaataca atcgggctga
     1081 gtaatttttt aagcggtgtt agcacaaagc tggcgaaaaa accgactttt tcagggtaaa
     1141 ttgccgctac tgttttaggt aggatttccg aaaaaaccaa cattacaaaa gttagcaaac
     1201 cggttgcaat tgccacaccg gcttcaccgg ataactgcat accgatcatc gttgcaatcg
     1261 cagaggcggc aatgttgact aagttattac aaattaagat aaggctaagt agcacatcgg
     1321 tgcgtgccaa gagtttttcc gccagtttcg cccctttatg cccttgctcg gcaaggtgac
     1381 gcattttgta gcggtttaaa gacatcaagc cggtttccga gcctgaaaaa aatgcagaga
     1441 gtactaaaag aatagctaag gtaataaata aactactcag gggaatactg tccaaaattc
     1501 aattcctatg taaattaatc ataaaaggta gcgtaacact aaaagcgttg tcactacctc
     1561 actttgtcgg ttcagccgac ttgcaaaaaa ttttgctttt ttgaccgctt gtagatcatt
     1621 aattgacgat caaacgacta ccgaaatagc cgatggtgag caacatcata cccgaaatcg
     1681 aataaataag cacccgtttt ccacgccaat gtaatttcca ctgtccaagt aactgaatgc
     1741 tataaacaag ccaagctaaa aaggagaaaa ttgctttatg aatatgctcc ggctcgaaaa
     1801 agttatgcaa ataaaccaca ccggaaaata aagtgagcgt cagcatagct tgtgcagcta
     1861 aggttaaagt aaagaaatgg cgttcaaccg tcattagcgg tggtagaatc tggcaaaaga
     1921 acattgtttt gctctttagt tttctatcca gccatttgag ttgaaaagca taaattaatg
     1981 ctaaaaagaa gagagcataa gaaaataatg caatccctaa atggaaaagc agtcccgcat
     2041 tttcttgcac attacggata aaactacctt ttacaaatcc agccaccccc acactacaaa
     2101 tggcgaaaga gtacacaata attaaaggaa accaaactgt tttccatttt aacaacgcaa
     2161 gtgtgacaca agccgccata atgaaactca tcaaagagat tacatttgtt accgtaaaat
     2221 tctgcccttc gattaaaaat aattcattat ataacacaga aaaatgcaaa atcgtggcaa
     2281 gtaacccaaa cccaaagact aacatcatat taggcttttt attaactgtt gcggcatttt
     2341 ctaaattcac tagagtaggg gcaacccaaa gtagcgctag agcgtaagca acaatggata
     2401 aaagaggaag taacatattg tctcgaatct cgtcaaataa gaataactat cagaatttta
     2461 atcaaaagta accgttttag ctagtttttg ctgttgctag atcaaaaatt atcctaaaaa
     2521 atgtgctttt cgccttaaat tgaaccctac cttttctcgg taagattatc tcactgaaaa
     2581 caataatttt cctttgacta atttgctttc tatctgtaat atgcacaccc tatgcaaaag
     2641 gtaaaactac cccttacgat tgacccttac aaagatgctc agcgtcgcgt tgattatgaa
     2701 ggctacattt caggtaaact tctaacacgc ttgggtgaat cagtatgtaa tgtgcaaagt
     2761 gatgcacaag tcactctctc gttatatgtc gatccacaac gcctaactgt gattaaaggc
     2821 acagcaaaag tagatgtgga atttgattgc caacgatgtg gtgacccgtt tacacaaacg
     2881 ctcgactgtt catttgtttt cagtccggtg tctaacatgg atcaggcgga catcttgccc
     2941 gaaatttatg aacctatcga agtaaacgag ttcggtgaag taaatttact agatatgatt
     3001 gaagatgaat ttattcttga attacctcta gtcccgatgc atagtgaaga acactgtgaa
     3061 gtgtccgtaa gtgaacaggt atttggcgag ttgccggaag aactggtaca aaaaccaaac
     3121 ccgttcgcag tattagctaa tttaaagaaa aactagatct aggagaaagc ccgtggctgt
     3181 tcaacaaaat aagaaatctc gttcacgtcg tgatatgcgt cgttcacacg atgcattaac
     3241 aactgcagca gtttcagtag ataaaaccag tggtgaaact cactttcgcc accacgtaac
     3301 tgcagacggt tactaccgtg gtcgtaaagt aatcaacaag taattccttt cgaggtttat
     3361 cttggttgat atcactcttg cagtagatgt gatgggcggg gactttggtc cccgcgttac
     3421 tattcctgca ctggaaaaag ccttaaaaca aaatccaagc ctttcttttt tattatttgg
     3481 tgatgaacac caaataaccc ctttattaca aaaacacaca ctactcaacc accccaaaat
     3541 ccaaattcat cataccgaac aagtgattga agctgatgtt ccgttcacta ctgcaatccg
     3601 ccaaagcaaa aacagctcta tgcggttagc gattgaggca gtaaaagagg gcaaagcaca
     3661 aggttgtgtt agtggcggta acactggtgc gttaatggga ttggcaaaat tattgattga
     3721 accattacca aatattgaac gccctgcact cacttcgcta attcctaccc tcaacggcaa
     3781 atccagcgta atgttagatt taggggcaaa tattgaagta agcaatcacc agctacaaca
     3841 atttgcagaa atgggaaacc tgtttgctca agtaatgctt gatttagttt accctcgcct
     3901 gtctttattg aatattggta cagaagaaaa taaaggcacg gcacaacttc aggcagtgca
     3961 tcaactgctg aaaaacagac aagacttaaa ttacaccggc tttattgaaa gcgataaatt
     4021 aaccagctat ctcactgatg tgattatttg tgacggctac accggcaata tcgcgttaaa
     4081 agcgttggaa ggggcagcta aaaatattat ttctctcttt aaaaaagaga aagccgattc
     4141 caatatttgc cgtaacacca aacgatattt actgaaagcg atgttctacc gttactaccg
     4201 caaattgcaa caaattaacc ctgaccgcca taacggggcg accttgctcg gcttatctaa
     4261 agtagtcgtg aaaagccacg gcggggcaaa tatcaatgcc ttttattacg ctattgatta
     4321 tgcggtgcaa caaattcaag gcgacattcc aaataaaatc ctagagggtt taacccaaaa
     4381 ataagcgaac tcaactgttc gcttattttc atttcttgtt tcacttaaag atacaagcgg
     4441 tcaaatttgc aaaaaaattt acccattcaa ccgcttgtgt ttagcctttt aattttgcca
     4501 ctcgtaaact aaccgcattg cggtgtgcat ctagcaattc tgcacttgcc attcgttcca
     4561 ccgtttcggc caaggctaga aagccttgtg gtgtcagctc ctgcaccgtc attcgcttgc
     4621 taaaatcagc taaacctaag ctcgaatagg ttttagtgta gccgtaagtc ggtaaaacgt
     4681 ggttagtgcc gctagcataa tcccccatac tttccggcga atacgcccct aagaaaatcg
     4741 agccggcatt atctaaatcc gccaacaacg agcgggcatc ttcagtttgc acaattaaat
     4801 gctcaggtgc atatttattg ctaatttcga ccgcttgttg caggttttct gcaataaaag
     4861 tacgacagtg agccaatgct ttacaggcag tttcagcacg aggcagaacc gacacctgct
     4921 tttctaattc ttcagcgact gcttttgcca agctctcact tggtgtgact aaaattactt
     4981 gggaatcggc cccgtgttcc gcttgagaga gcaagtcgct cgccacaaat tcagggtcgg
     5041 caaactcatc ggcaatcacc aacacttccg acgggcctgc cggcatatca atcgccgtgc
     5101 cttcttgcac aacttggcgt ttcgcctccg tcacaaacgc attgcccggg ccgaaaattt
     5161 tatccacttt cgctaccgtt tgtgtgccat ttgccatcgc aataatcgct tgagcccccc
     5221 caactgcgta aatcgtttcc accccacaca aatgggcggt gtagagaatt tcatcggcaa
     5281 tcggcggtgg tgagcaaagc acaatcttgt tacaaccggc aatttttgcc ggcactgcaa
     5341 gcattaacac ggtagagaaa agcggggctg aaccgcccgg aatataaagc cccaccgcat
     5401 taatcgggcg agtgatgact tggcaacaca cccccggctg ggtttcaata tccacctctt
     5461 gattttgctg tgccttgtgg aaagcctcga tattcgcttt cgcattttga atcgcttgct
     5521 taagctctgg tgaaatacgg ctctgtgctt gctgaatctg ctgttctgat acaactaagc
     5581 tctctaattt tactttatcg aacttttcag ccaattcaaa taatgcctta tcgccgttgg
     5641 caattacatt cgctttaatt tcattaactg ccgctttaat actctcgcca accacctgtg
     5701 ccggacgggc aagatattgc tgctgctcag tttctgttaa attgttccaa attagggttt
     5761 gcatatttat tccttttacg ccaacatttt ctcaatcggt aaaaccaata cggaacttgc
     5821 gcccatttct tttagctgct ccattgtttc ccagaacagg ttttcttggc tgacaacgtg
     5881 cattgccaca cgggtttggt cgttagctaa cggtaaaata gtcgggtttt ccacaccggg
     5941 taataaagcg gtaatttccg ccagtttgtc tttgggggcg tgcagcataa tatacttaga
     6001 ttcgttcgcc tgttgcacac cttgaatgcg ggttaataat ttatccacta acgcttgttt
     6061 ttccgctgaa agtggttctt ggcgttgaat taggcaagca gttgaacggt aaatcacttc
     6121 aacctctttt aatccgttcg cctctaatgt tgcacctgaa gaaaccaagt cacaaatcgc
     6181 attcgcaatt ccggcacttg gagcaacttc aaccgaaccg gtgagtaaaa tacttttaaa
     6241 cggcacattt tgctctttca tataccgctt taataaattg gggtaagaag tggcaatcac
     6301 agcatcttct aaatctttga tattgttata atttttatca cgatcaatag ctaacgataa
     6361 acggcagcca ccaaaatcta actgacgaag ttttttatag gcaacattct caccagcact
     6421 taatcgccct aattccactt cctctaatac attttcaccg ataatcccaa gatcaactac
     6481 gccgtcaaaa actaagcctg gaatgtcgtc atcacgcacc cgtaaaattt caatcggcat
     6541 attttctgaa taggcaatca ggcgttgatc gttccaaatg attttcacgc cacattgttt
     6601 aagcaactca ctacattctt ggcttaagcg accttttttc tgcatcgcaa ttcttaatcg
     6661 attttgtgtt gtcattgttg tttccttctt atgttttaaa ttgtaaaaat aaaaaaacct
     6721 ctcgaaatca cttccgagag ggttaaattt atgctgcgtt taggcgtact cggaagtctt
     6781 atcttccgca cataccaaat gcccgaaaga ttattcaggt aaatgatggt ggtgatgagt
     6841 acgaataaaa gccattttgt tgttctccaa actaaattgc tttaaaagat acgcttaaat
     6901 tttggcaatg acaagtgttt tttgcgaaaa aattgctatt tttcatctct ctaaaacaac
     6961 tatcgcaaac gtttgcgtaa agcggtataa tttgcctgat tttttgcaaa ttcaacaaag
     7021 taggaataac actatgcaaa tcagcctcaa aaagatctac tccggcaaag tccgtgatct
     7081 ctacgaaatc gatgaaaaac gtatgcttat ggttgccacc gaccgattat ctgcctttga
     7141 tgttatttta gacgatccta ttcctcgtaa aggcgaaatc ctcacccaaa tctccaactt
     7201 ctggttcaac aaattagccc acattatgcc aaaccacttc accggcgaca gtgtgtttga
     7261 cgtgttaccg caagaagagg cagaagctat tcaacatcgt gcggtggttt gcaaacgctt
     7321 aataccggtg aaaattgaat cgattgtgcg gggttattta accggctccg ggctgaaaga
     7381 ttaccaacaa actggcacga tctgcggctt aacattgccg gaaggcttgg ttgaggccag
     7441 caaattaccg caaccgatct tcacaccgtc cagcaaagcc gaggtgggcg atcacgatat
     7501 taatatcagc tacgaagagt gtgaacgcca gatcggtcaa gaacttgccg ccaaagtgcg
     7561 tgatgccgct ctaaaactct acactgaagc ggcaaactat gccctgacca aaggaattat
     7621 tatttgcgac accaaattcg aattcggctt agacgaaaac ggtgaactaa cgctaatgga
     7681 cgaagtccta accccggatt ccagccgttt ttggtcagtt gaaacctacc gagaaggcac
     7741 aaatccacct tcatttgata aacaattcgt gcgtgactgg ctggaaaata gcggttggaa
     7801 caaacaaccc ccagccccga aaataccggc agaggtaatt gaaaaaaccg tagcgaaata
     7861 tcaagaagca ttggatttat taaccaaata acaccacaag cggtcaaatt tacgctattt
     7921 tttgcaaaac aaaaacctgt aagccaacac ttacaggttt tttagttagt ggtaaaaagt
     7981 gcggttattc taccgcactt ttgcaaaatt ttgtgtaaat ctgaccgctt gttagccttg
     8041 cgaagaaata cgtttcatat ctgtcatata accacgtaat tcttgaccga ttaactcgat
     8101 tgggtggtta cggattgcgt cattcacatc acgtaaggta acgttgtcga tttccactgc
     8161 cggagttggt tcacctaaat cgcctttctc taacgttggg ataattttct ccgctaaaat
     8221 cggggttgca acgtgagaga ataagtagtt accgtattct gcggtatctg aaattaccac
     8281 gttcatttca tataaacgct tacgagcgat tgtatttgcg attaacggta actcgtgtag
     8341 agactcgtag taagccgatt cttcataaat accggctgaa accattgtat caaaggctaa
     8401 ttcaacccct gctttcacca ttgcaaccat taacacaccg ttatcaaagt attcttgctc
     8461 agaaatttta ataccgtctg cttgtggtgc gttttcaaag gctgttttac ctgtttcttc
     8521 acgccatttt aataagttgg catcgccgtt tgcccagtct gccatcatag ttgatgagaa
     8581 ctcaccactg atgatgtcgt ccatatgttt ttcaaataag aacgctaatt cttctttgat
     8641 ttgctcagaa agctcgaacg cacgcaattt ggcactgttt gataagcgat ccatcattaa
     8701 ggtgataccg ccttgtttta acgcttcggt aatggtttcc caaccgtatt ggatcaattt
     8761 acctgcgtaa gccggatctt taccttccga cactaatttg tcgtagcaca cgattgaacc
     8821 tgcttgtaac ataccgcaaa gaatggtttg ttcacccatt aaatcagatt tcacttctgc
     8881 tacgaatgaa gattctaata cgcccgcacg gtcaccaccg gtagcacttg cccacgcttt
     8941 cgcaatggct aaaccttcgc ctttagggtc attttccggg tgaacggcaa ttaaggttgg
     9001 cacaccgaaa ccacgtttgt actcttcacg cacttccgtg cccgggcatt tcggagcggt
     9061 catcacaacc gttaaatccg gacgaatttg ctcaccttct tccacaatgt tgaaaccgtg
     9121 tgaataacca aatgccgcac cttgtttcat taacggcatt acatcggcaa ctactttaga
     9181 gtgctgtttg tccggcgtta agttaatcac taaatccgct tcaggaatta attgctcgta
     9241 agtacccacc acaaagccgt tttcagaagc acgtttaaat gaagcacgtt tttcagcaat
     9301 cgcttcggca cgtaaagcgt aagcaatatc taagcctgaa tcacgcatat ttaaaccttg
     9361 gtttaaacct tgagcaccac aacccacaat cacgattttt ttgcctttta agaaattgca
     9421 gccatctgca aattcatcac gatccataaa gcggcaacga cctaactggt ctaatttttg
     9481 acgtaaattt aaagtgttga aatagttagc cattttttaa tccttatttg aattaatgag
     9541 attgatatga tgttttgttt gcacattgtt atagtttgat ttgattatac cttaacctaa
     9601 tattgcgtaa attgaaaata ttaatatttc atattgcata aaatgcaact tgcaaaaatt
     9661 tt
//
LOCUS       DANJWC010000088         9604 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.088, whole genome shotgun sequence.
ACCESSION   DANJWC010000088 DANJWC010000000
VERSION     DANJWC010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9604
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.088"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            2..598
                     /gene="nfuA"
                     /locus_tag="PWX02_001532"
     CDS             2..598
                     /gene="nfuA"
                     /locus_tag="PWX02_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HDL1729530.1"
                     /translation="MEEQTIHISISDAAQAHFARLLEQQEEGTNIRIFVVNPGTPGAE
                     CGVSYCPPNAVEETDTEFKFNSFSAFVDDISLPFLDEAEIDYVTDPMGSQLTLKAPNA
                     KMRKVADDAPFIERLDYVIQTQVNPQLASHGGKVTLIEITEDKYAVLQFGGGCNGCSM
                     VDVTLKEGIEKQLLAMFPDELVGVKDITEHQHGEHSYY"
     gene            complement(767..2404)
                     /gene="pyrG"
                     /locus_tag="PWX02_001533"
     CDS             complement(767..2404)
                     /gene="pyrG"
                     /locus_tag="PWX02_001533"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HDL1729531.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLASILEARGLNVTIMKLDPY
                     INVDPGTMSPTQHGEVFVTEDGAETDLDLGHYERFIRSKMSKANNFTSGKIYSEVLRK
                     ERRGDYLGATIQVIPHITNEIKARVLEGGKGRDVAIVEVGGTVGDIESLPFLEALRQL
                     AVDIGREKTLFMHLTLVPYIPTAGEVKTKPTQHSVKELLSIGIQPDVLICRSDRAIPE
                     NEKKKIALFCNVPERAVISLKDVDSIYRIPELLKSQGLDSFVCDRFRLDCKEADLSEW
                     EQVLYRQANPTGEVTIGMVGKYVELPDAYKSVNEALKHAGLTHRLTVNIKYIDSQDIE
                     TKGTELLAGLDGILVPGGFGYRGVEGKIRTAQYARENKIPYLGICLGMQIALIEYARN
                     VAGLTEANSSEFDKDCSQPVIGLITEWQDAEGNVETRSDESDLGGTMRLGSQQCHLIE
                     GSKARELYGAETIVERHRHRYEVNNKLLPQIEAAGLKVSGLSADRKLVEIIEVPNHPW
                     FIAAQFHPEFTSTPRDGHPLFAGFVKAAKDHQESRKA"
     gene            2668..3717
                     /gene="ansB"
                     /locus_tag="PWX02_001534"
     CDS             2668..3717
                     /gene="ansB"
                     /locus_tag="PWX02_001534"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HDL1729532.1"
                     /translation="MKIKKLLLPTLLSIATMPIQAADLPNITILATGGTIAGSQKNTA
                     TSAYQAGQLNIETLIEAVPDIKKLANIKGEQIVKIGSQDMSDEVWLKLAKAINQQCPN
                     TDGFVITHGTDTMEETVYFLDMTVKCEKPVVLVGAMRPATEKSADGVLNLYNSVVVAK
                     DSKSSGKGVLVAMNDIVLGARDVTKTSTTSVQTFNSPNFGALGYIHNSKVDYERSPES
                     KHTVNTPFKVDELTELPKVGIIYAYSNMPTEPLQALLDASYQGIVVAGVGNGNMNAAN
                     LALLEEAAKKGITVVRSSRVPTGYTTRDAEIDDSKYGFIASGTLNPQKARVLLQLALT
                     QTNDIKTIQQYFEDF"
     gene            complement(3791..4435)
                     /locus_tag="PWX02_001535"
     CDS             complement(3791..4435)
                     /locus_tag="PWX02_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729533.1"
                     /translation="MRIWQKLASVIVVTLGLISNASAENFKLKYSSNYLMPAYINFNN
                     GGGQYNIQAKINVPLYNIVFSAKGTEKNEQFHMLSYRDTRNGKTYAMAEIDSKTVKYG
                     KVKEPLKEEALTMPTYDLFTTAFQLSYYDKLPSSFQTTNGKKLYPTPNVQLRKAQKEV
                     KVGGKTYQEITYKFRTSDKKDITVKKFEGEKFPRYIAYSRDGDNYELTFDSFVK"
     gene            complement(4436..5419)
                     /gene="cysB"
                     /locus_tag="PWX02_001536"
     CDS             complement(4436..5419)
                     /gene="cysB"
                     /locus_tag="PWX02_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HDL1729534.1"
                     /translation="MKLQQLRYIVEIVNQNLNITEAAEVLYTSQPGISKQVRLLESEL
                     GINIFERNGKHVKGLTPAGEKIVAIARELLVKAQSIKSVAEEQTRPNKGILRIATTNT
                     QARYMLPSVIEKFKARYPEVSLHLHQGSPNQIYDALVAGEVDLAITTEAQYLFDEAVL
                     LPCYWWNRSVIVKPEHPLAAFVNQSKNLTVEQLGRYDLITYTFGFTGQSDLDHAFNKE
                     GILPNIVFTATDADVIKTYVRLGLGVGIMASMAHTETDKDLVAINASHLFQPSMTQIA
                     FKRGTFLRNYMYDFINYFSPHLTRVQVEKAEQLRDNNAISRLFESISLEEK"
     gene            complement(5416..6099)
                     /gene="yjjG"
                     /locus_tag="PWX02_001537"
     CDS             complement(5416..6099)
                     /gene="yjjG"
                     /locus_tag="PWX02_001537"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HDL1729535.1"
                     /translation="MKYQWVLFDADETLFSFNSYLGLKAMLARYQIDFSEQDYQDFQA
                     VNKPLWVAYQNNEITAQDIQTRRFAKLSAQTGVDPLQLNQELMAEMAFVSQPLEGVMD
                     MLNALYGKVKMGIITNGFTDLQQKRLDNTQTSHFFDIVVISEQIGAAKPDRQVFDYAF
                     ALMDEFDRTKVLMVGDTLASDILGGNNAGIDTCWLNLLSKENDTDIKPTYEVHTMQQI
                     IAIVEGKSL"
     gene            complement(6175..7068)
                     /locus_tag="PWX02_001538"
     CDS             complement(6175..7068)
                     /locus_tag="PWX02_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HDL1729536.1"
                     /translation="MNKKLPPLNALKAFESAARHLNFTKAAEDLFVTQAAVSHQIKLL
                     EDFLGASLFHRRNRLLELTELGAQYFYEVQPLLEQIALATDKIRQQSSRQILTISVPQ
                     TFGMHWLVPRLADFHEKYPEVEVRIKGVDQDEGLLGKETDVAIYYGKGDWQGLESIRL
                     TESRIVILASPEYLSQNTIHLPADLAGKHLLHSSTHNKWKRMVEHLHIEDIVDAETGS
                     IFSHTFMVLQAAMHQQGIAIANQLLAQHEIEKGTLIEPFPTTLFDEKSFYVVYPSQSA
                     QVQKVQFFVEWIQQKMKECCA"
     gene            7514..8551
                     /gene="rsgA"
                     /locus_tag="PWX02_001539"
     CDS             7514..8551
                     /gene="rsgA"
                     /locus_tag="PWX02_001539"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HDL1729537.1"
                     /translation="MSKPKLTHNQQRRIQSNHHKRINKKEIEWQDEMLGEMQQGIVVT
                     RHVKHADVETAEGQIFRCNIRRTLKNVVVGDQVSWRKGSEQLQGISGVIEAVFPRKNE
                     LTRPDYYDGIKVMASNIDQIIIVSAVLPTLSLNIIDRYLVICETANIPALIVLNKIDL
                     LNESERQAVKEQLKIYEEIGYETLCLSADTGENMQALHQYLAKGTSIFVGQSGVGKSS
                     LINQLLPEVNALTGSVSDNSGLGQHTTTASRLYHLPQGGQLIDSPGIREFGLWHLEPE
                     QITLGYREFQSVLGTCKFRDCKHKTDPGCALRQAVENGEISAIRFENYHRLIESLSET
                     KSQRHFRKEEV"
     gene            8548..9147
                     /gene="rnhB"
                     /locus_tag="PWX02_001540"
     CDS             8548..9147
                     /gene="rnhB"
                     /locus_tag="PWX02_001540"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDL1729538.1"
                     /translation="MMNEFIYPNARLIAGVDEVGRGPLVGAVVTAAVILDPNNPIQGL
                     TDSKKLSEKKRQALAEEIKAKALSWSLGRAEPTEIDELNILNATMLAMQRAVAGLHIQ
                     PDFVLIDGNRPPKLPMPAQAVVKGDSLVAEISAASIIAKVARDQEMDELDQQFPQYGF
                     AQHKGYPTKLHFEKLAEFGATPYHRKSFAPVRKVLGLID"
     gene            9160..9519
                     /locus_tag="PWX02_001541"
     CDS             9160..9519
                     /locus_tag="PWX02_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HDL1729539.1"
                     /translation="MTTEYQETIFTKIINKEIPANIVYQDELVTAFRDIAPQAPTHIL
                     IIPNKFIPTVNHVSNEDELALGRLFTVAAKLAQEEGIAEEGYRLVMNCNKNGGQEVFH
                     IHMHLLGGQKLGPLVAK"
BASE COUNT         2932 a         2022 c         1817 g         2833 t
ORIGIN      
        1 aatggaagaa caaaccattc atatttcaat atcagatgca gcacaagccc attttgccag
       61 actattagaa cagcaagaag agggaactaa tatccgtatt tttgtggtca atcccggcac
      121 accgggggca gaatgcggtg tctcttactg cccacccaat gcagttgaag agacggatac
      181 cgaatttaaa tttaacagtt tctctgcatt tgtcgatgat attagcttac cgtttttaga
      241 cgaagcggaa attgattatg taaccgaccc aatgggttca caacttaccc tcaaagcccc
      301 taacgccaaa atgcgtaaag tggctgatga tgcaccgttt atcgagcgtt tagattatgt
      361 gattcaaacc caagtcaatc cacaacttgc cagccacggc ggtaaagtga ccttaattga
      421 aatcaccgaa gataaatatg ccgtgctaca attcggcggt ggttgtaacg gttgctcaat
      481 ggtggatgtg accttaaaag agggaattga gaaacagctt ctggcaatgt tccctgatga
      541 attagttggt gtgaaagata ttaccgaaca ccaacacggt gaacattctt actattaaaa
      601 atactaaaag ggataaatca tttgatttat ccctttctta taattaatga gcaagcggtc
      661 aaatttactc gctttcgctc gcacttcgtg ccatcagcaa agccgatgtt caaacgcaag
      721 cgtttgtaca aaaaaatgag taaattcgac cgcttgttaa gtttgattaa gccttacgac
      781 tctcttggtg atctttcgca gctttcacaa aacctgcaaa taatggatga ccatcacgcg
      841 gagttgaggt aaattccgga tggaattgtg ctgctataaa ccaagggtga tttggcactt
      901 caataatttc cactaattta cgatctgccg ataaaccgct cacttttaag cctgccgctt
      961 caatttgtgg taacagctta ttgtttactt cgtaacggtg gcgatgacgt tcaacaatcg
     1021 tttccgcacc atataactca cgagcttttg agccttcaat taaatggcat tgttgtgagc
     1081 ctaaacgcat tgtaccacct aaatctgact catcgctgcg ggtttccaca ttcccttccg
     1141 catcttgcca ttcggtaatt aagccaatca ccggctgtga gcaatcttta tcaaattcag
     1201 aagaatttgc ctcggttaaa cctgccacat tacgggcata ttcaattagg gcgatctgca
     1261 tacctaaaca gatacctaag taaggaatct tgttttcacg ggcatattgt gcggttcgaa
     1321 ttttgccttc cacgccacga tagccaaagc cgcccggcac aagaatgccg tctaagcctg
     1381 ccaataactc tgtgcctttg gtttcaatat cttgtgaatc aatgtattta atattcactg
     1441 ttaaacgatg cgttaaacct gcgtgtttca aagcctcatt caccgattta tacgcatcag
     1501 gtaattccac atattttccg accataccga tagtcacttc gccggttggg tttgcttggc
     1561 ggtatagcac ttgttcccac tctgaaagat cggcttcttt gcaatctaaa cggaaacgat
     1621 cgcacacaaa ggaatctaac ccttgcgatt tcaataattc cggaatgcga taaatcgaat
     1681 ctacatcttt aagcgaaata accgcacgct ccggcacatt acagaaaagg gcgatttttt
     1741 tcttctcatt ttccggaata gcacgatctg aacggcaaat taacacatcc ggctgaatac
     1801 cgattgaaag caactctttt actgaatgct gagtcggctt agttttcact tcccctgcgg
     1861 ttggaatata aggcactaag gttaaatgca taaacaaggt tttttcacga ccgatatcca
     1921 ccgctaattg gcggagtgct tctaagaatg gcagtgattc aatatccccc accgtgccac
     1981 ctacttctac aatggcgaca tcacgccctt taccgccttc taacacacgg gctttaatct
     2041 cgttggtaat atgcggaata acctgaatgg ttgcacctaa atagtcgcca cggcgttctt
     2101 tacgcaacac ttcagagtaa attttaccgc tggtaaagtt gttggctttg ctcatttttg
     2161 aacggataaa acgctcgtag tgacctaaat ccaaatcggt ttccgcaccg tcttcggtca
     2221 caaatacctc cccgtgctga gttggactca ttgtacccgg atcgacattg atataaggat
     2281 caagcttcat aatggttaca tttaaaccac gagcttccag aatagatgct aaagaggctg
     2341 ccgcaatacc cttccctaaa gaagatacaa caccgcctgt gacgaaaata taattcgttg
     2401 tcattgtgat tgcctttgct gattgattag gttggaaatt cagaatatca gtttacttcg
     2461 taaaaaagaa actatcagga cgggaaagta gtttacatca aattacattt ttttgctaat
     2521 ggaaaatcgg caagcagtca gattaattct tttttcaata atgccgacta atcttaatta
     2581 atacactatt atttgcgata gatcagaatt aacctacatg tttatagata tcataatacc
     2641 tgtttttcta atactggaga cgatacaatg aaaatcaaaa aattactact gccaactctg
     2701 ttaagcattg ctactatgcc aattcaagca gccgacctac ctaatattac aattcttgca
     2761 actggaggga caattgccgg tagccaaaaa aatacggcaa cctcagctta tcaagctggg
     2821 cagctaaata ttgaaacact catagaagcc gtcccggata ttaaaaaatt agcaaatatc
     2881 aaaggagaac aaatcgttaa aatcggatct caggatatgt ctgatgaagt atggctaaaa
     2941 ctggcaaaag caattaatca acaatgccca aacacagatg gcttcgttat tacgcacggc
     3001 acagacacta tggaggaaac cgtatacttc cttgatatga ctgtaaaatg cgaaaaaccc
     3061 gtagtattag tgggggccat gcgaccagca actgaaaaaa gtgccgacgg cgtattaaat
     3121 ttatataact cagttgttgt cgccaaagat tctaaatcta gtggcaaagg ggtgttggtt
     3181 gcaatgaatg atattgtatt gggcgctcgc gatgttacaa aaaccagtac tacatcagtg
     3241 caaacattta actcaccaaa ctttggcgca ctaggctata tccataatag caaagttgat
     3301 tatgagcgct ctcctgaaag taaacacacc gtaaacaccc ctttcaaagt agatgaatta
     3361 acggaattgc caaaagtcgg cattatttat gcttattcaa atatgccaac cgaaccgctt
     3421 caagcattat tagatgcgag ttatcaaggc atcgtggttg caggagttgg caatggcaat
     3481 atgaatgcgg ctaatttggc tttacttgaa gaagcagcta aaaaaggaat taccgtggtt
     3541 cgctcgtctc gagtgccaac cggatatact acccgagatg cagaaattga tgatagcaag
     3601 tatggtttta ttgcttccgg cacattaaat cctcaaaaag ctcgagtatt actgcagctc
     3661 gccttaacac aaacaaatga tataaaaact attcaacaat attttgaaga tttctaaaaa
     3721 gtaaataaca agcggtctat tttgcaaaac aaatcacaaa atagaccgct tgtctcatta
     3781 ctaatcttta ttattttaca aagctatcaa aagttagctc ataattgtcg ccatcacgac
     3841 tataggcgat atagcgaggg aatttttcac cttcaaattt tttcaccgta atgtcttttt
     3901 tatcactggt tctaaacttg taggtaattt cttggtaggt tttgccgcct acttttacct
     3961 ctttttgggc tttgcgtagc tgcacattcg gtgttgggta aagtttttta ccgttagtag
     4021 tttgaaaact gctcggtaat ttatcgtaat agctcaactg aaaggctgtg gtgaacaaat
     4081 cataagttgg catagtcagc gcttcttctt ttagtggctc tttcactttg ccatatttta
     4141 ccgttttaga atcaatttct gccatcgcat aagttttgcc gttacgggta tctcggtagc
     4201 tcaacatatg aaattgttcg tttttttctg ttcccttcgc agaaaagaca atattatata
     4261 aaggcacatt aatttttgcc tgaatattat attggccacc accgttatta aagttaatgt
     4321 aggctggcat taaataattt gagctgtatt tcaacttaaa attctccgca ctggcgtttg
     4381 aaatcaagcc caaagtgacc acgattacgc tcgctaactt ctgccaaatt ctcatctatt
     4441 tctcctctaa agatatactt tcgaacaacc ttgaaattgc attgttatct cgtaattgtt
     4501 ccgctttctc cacttgtacc cgtgttaaat gtggcgaaaa ataattaata aaatcataca
     4561 tatagttacg aagaaaagtt cctcgtttaa aggcgatttg cgtcatactc ggttgaaaca
     4621 ggtgacttgc attgatagcg actaagtctt tatccgtttc cgtatgtgcc attgatgcca
     4681 taataccaac ccctaagcct aagcgcacat aagttttgat tacatcagca tctgtcgccg
     4741 taaatacgat attaggcaaa ataccttcct tattgaaggc atgatctaaa tccgattgcc
     4801 ctgtaaaacc aaaagtatag gttatcaaat catatctacc taattgttcg acagtcaaat
     4861 ttttactttg gttcacaaaa gccgcaagag gatgctctgg tttgacaata actgaacgat
     4921 tccaccaata acacggcaat aaaaccgctt catcaaataa atattgagct tctgtggtaa
     4981 tggctaaatc cacttctcca gccaccaaag catcataaat ttgattcggg gagccttggt
     5041 gcaaatgcaa acttacttca ggatagcgag ccttaaactt ctcaattaca ctcggtagca
     5101 tataacgggc ttgggtattg gtggtcgcaa tgcgtaagat gcctttgttg ggtcgagttt
     5161 gttcttcagc aaccgattta atgctctggg cttttaccaa taattcacga gcaatcgcca
     5221 caattttttc accagcaggc gttagcccct taacgtgctt accgttacgc tcaaaaatat
     5281 taattcccaa ctcactttcc aacaaacgaa cttgtttgct aatccccggt tgagaagtgt
     5341 aaagcacctc tgcggcctcg gtaatattca agttctggtt tactatttca acgatataac
     5401 gtaactgttg tagcttcata atgatttccc ctctacaatc gcaataatct gttgcatcgt
     5461 atgcacttcg taagttggct taatatccgt atcattctct ttactaagca aatttaacca
     5521 acaagtatca attccagcat tattacctcc taaaatatca gaagcgaggg tatcgcctac
     5581 cataagcact ttagtacgat caaattcgtc cattaatgca aaagcgtaat caaacacttg
     5641 gcgatcaggc tttgcggcac caatttgttc tgatatcact acaatatcaa aaaaatgtga
     5701 agtttgagta ttatctaaac gtttttgctg caaatcggta aaaccattgg tgataatgcc
     5761 catttttact ttaccatata aagcatttaa catatccatt acaccttcta aaggctgact
     5821 gacgaatgcc atctccgcca ttaactcttg attaagttgt agcggatcta cgcctgtttg
     5881 agcagataat tttgcaaaac gtctggtttg aatatcttga gcagtgatct cattattttg
     5941 gtaagccacc caaagtggtt tatttaccgc ttgaaagtct tgataatctt gttcactaaa
     6001 atcaatttgg taacgagcca gcatcgcttt taaaccaaga taagaattaa atgaaaacaa
     6061 ggtttcatca gcatcaaata atacccattg atatttcata atattccttt tttaacttag
     6121 ataacaagcg gtcaaatttt tctaattttt cgcataccgc tgataaagcc acgcttaagc
     6181 acaacactct ttcatttttt gctgaatcca ctcaacaaaa aattgtactt tctgaacttg
     6241 ggctgattgc gaaggataga caacatagaa cgatttttca tcaaacaatg tggttgggaa
     6301 aggctctatt aaagtacctt tttcaatttc gtgctgtgcc aacaactggt tggcaatcgc
     6361 aatgccttgt tggtgcattg cagcttgaag caccataaaa gtatggctaa aaattgagcc
     6421 agtttccgca tctactatat cttctatatg caaatgttca accattcttt tccacttatt
     6481 atgggtggag gaatgtaata aatgcttacc tgctaaatcc gccggtaaat gaatggtatt
     6541 ttgcgataaa tactcaggcg aagctaaaat aacaatacgg gattccgtca aacggataga
     6601 ttccagacct tgccaatccc ccttaccata ataaatagca acatctgttt ctttacctaa
     6661 taatccctca tcttgatcaa cacccttaat acgtacttcc acttctggat atttttcgtg
     6721 gaaatccgcc aaacgtggga ctaaccaatg cataccaaat gtttgtggta cgctaatagt
     6781 cagaatttga cgactacttt gctgcctaat tttatctgtt gcgagagcaa tttgttctaa
     6841 taatggctga acttcataga aatattgtgc gcctaactca gttaattcca gcaaacggtt
     6901 acggcgatgg aacagggaag cacctaaaaa atcttctaat agtttaattt ggtggctcac
     6961 tgcagcttgt gtgacgaaaa gatcttctgc cgctttagta aaatttaaat ggcgtgcagc
     7021 agattcaaat gctttcaatg cattgagtgg tggaagtttt ttgttcataa tttttcaatc
     7081 taatagatta ctaaacaagc aactctatta ttttttctga tagtttaaat aaaaaaaatt
     7141 cgtttgatgc ttgcaaatag aaataataaa atatccgcat acttagatgg atagtttaga
     7201 atttatttct attcctaaat tttaagtatg atgttgtgtt tgcatattgg tctaggaaac
     7261 tagactagag taacgaaagt tacttgtttc acttcctgta tattttgaac cgtattggtt
     7321 tataaccgcc cactttgggc ggtttttttc tatgtaaaaa aacagcacaa aacctagatc
     7381 gcattctata ttattcgcta cctttattct agcctttctg cttttttatt ctttcacttt
     7441 aatctattat aaaaagaggt tttatagtat gctaaagcct atttttcatt tgcaataatt
     7501 accgagagtt cttttgagta aaccgaaatt aacccataac caacagcgta gaatccaatc
     7561 taaccatcat aagcgtatca acaaaaaaga gattgaatgg caagatgaaa tgttaggaga
     7621 aatgcaacag ggcatcgtcg ttactcgcca cgttaaacac gctgatgttg aaaccgcaga
     7681 ggggcaaatt ttccgctgta atatccgccg tacattgaaa aatgtagtgg tgggcgatca
     7741 ggtttcttgg cgtaaaggta gtgagcaact acaagggatt agtggggtca ttgaagccgt
     7801 cttcccacgt aaaaatgaac ttacccgccc cgattattat gacggtatta aggtgatggc
     7861 atcgaacatc gatcaaatca ttattgtatc agctgtgcta cctacacttt cgctgaatat
     7921 tatcgaccgt tatttagtga tttgcgaaac ggcgaatatt cctgccttaa tcgtgctaaa
     7981 caaaattgat ttattaaacg aaagcgaacg gcaagcagtt aaagagcagc tcaaaatcta
     8041 tgaagagatt ggctatgaaa ccctatgttt atcggctgat acgggtgaaa atatgcaggc
     8101 gttacaccaa tatcttgcta aaggtacgtc tatttttgtc ggacaatccg gcgtggggaa
     8161 atccagccta attaaccaac tgttacctga agtcaatgct ctcactggct cagtgagcga
     8221 taactcaggg ttaggtcagc acaccaccac tgcctctcgc ttataccatt taccgcaagg
     8281 cggacagctg attgactcgc cgggcattcg agaatttggc ttatggcatt tagaacctga
     8341 gcaaatcacc ttaggctatc gagaatttca atcggtttta ggcacgtgca aattccgtga
     8401 ttgcaaacac aaaaccgacc cgggttgtgc cttaagacaa gcggtcgaaa atggcgaaat
     8461 ttctgcaatt cggtttgaaa attaccatcg cttaattgaa agcctgtctg aaaccaaaag
     8521 ccaacgccat ttcagaaaag aagaagtata atgaatgaat ttatttaccc taacgcccga
     8581 ttaatcgccg gtgttgacga agtcggacga ggacctttag tcggagcggt tgtgaccgca
     8641 gccgtgattt tagatccgaa taacccgatt cagggcttaa ccgattccaa aaaattatcc
     8701 gagaaaaaac gccaagcttt ggcagaagag attaaagcca aggctctgag ctggtcgcta
     8761 ggacgtgccg agccgacaga aattgatgag ctgaatattt taaatgcgac aatgttggca
     8821 atgcaacgtg cggttgccgg cttgcatatt cagccggatt ttgtgctaat tgacggcaat
     8881 cgtccgccaa aactgccaat gccggcacaa gcggtggtga aaggagacag cttagtggct
     8941 gaaatcagtg ctgcttcgat tattgccaaa gtcgcccgag atcaagaaat ggacgaatta
     9001 gaccaacaat ttccgcaata cggcttcgca cagcacaaag gctacccgac taaactccat
     9061 tttgaaaaat tagcagaatt tggggcaaca ccttatcatc gtaagagttt tgcccctgta
     9121 agaaaagtat taggtttaat cgattagaag gaaaacacaa tgactacaga gtaccaagaa
     9181 acgattttca ccaaaatcat caataaagaa attccggcaa atattgtcta tcaagatgaa
     9241 cttgtgaccg catttcgtga tattgctcca caagcaccca cgcatatttt gattattccg
     9301 aataaattta ttccaaccgt caatcacgtg agtaatgaag atgaattagc cctagggcgt
     9361 ttatttacgg tggcggcaaa attagcccaa gaagaaggca ttgccgaaga aggctatcgc
     9421 ttagtgatga attgtaataa aaacggcggg caggaagtgt tccatattca tatgcacttg
     9481 ttaggcggac aaaaactagg acctttagtg gcaaaataac tatgcgtatt tcctactttg
     9541 catttgcaat tttttcgatt tttttgaccg cttgtggtag caaacctgtc agctatttac
     9601 caac
//
LOCUS       DANJWC010000089         9543 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.089, whole genome shotgun sequence.
ACCESSION   DANJWC010000089 DANJWC010000000
VERSION     DANJWC010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9543
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.089"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(70..2262)
                     /gene="yccS"
                     /locus_tag="PWX02_001542"
     CDS             complement(70..2262)
                     /gene="yccS"
                     /locus_tag="PWX02_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010129387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HDL1729540.1"
                     /translation="MNKFLRYLQHSWLSENVIKTLPMFISLNIAAVSIWQLGISDFAM
                     PLMLGIIAGGLVDLDNSLIGRAKNLIISLVAFSLSSIGASVSLHLGWLFVPVAVVCTF
                     LLVMLGAIGQRYSTIAFGTLIVAVYNSLAYTPEVLWYHNVVMILLGTLLYGLVGILVY
                     LIFPNRTVQENLASAYLALSHYLLAKSAYFDPDDDDLAAKQLALAKANSEVMAAFDKT
                     RVALFYRLRRQHKQQRTQRMLRYYFTAQDILERASSSHYQYHELFQQLSNSDLMFRFQ
                     RVLELQAAACQKIAMSLRHREVYEHGMCGEKALQGLLNSLNYHQERGLKSAYRWRAIA
                     ENLGNIERQLAQIEQGSSSGEHIEPLSKNFTKSHRLIAENITGLSNISQAIKAHLTFE
                     SQLFRHAVRLSIVVLLCSAIVPILGFDSKGYWILLTAIFVCQPNYTATKTRLIQRVIG
                     TIFGVLVGGSFELFNVTLSLEAQLGLIVLTGSLYTFFRLVHYGFSTFYITVLVMISLD
                     IAGVSLESGILMRMLDTLLGTAIAWLAVAFLWPDWKYLNLQRNLHNSLKASGQYLRHI
                     IAQLQFGYNEQLPYRIARRDVHNHLSALSNAITNMYSEPQKYANDLTFAPKLLSIAYT
                     LLGYISTLGAYRIASREINQQVDFSAMFFSLAREVANIIGQIASESRSFAKLSEKLTA
                     IDQDLAQFEQENQQKQNDLSLVLIQQLRLIVQLLPQMQSLLKAEICHQ"
     gene            complement(2306..2764)
                     /locus_tag="PWX02_001543"
     CDS             complement(2306..2764)
                     /locus_tag="PWX02_001543"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDL1729541.1"
                     /translation="MKTTVRTIAKQKQIALVAHDSCKQDLIRWTQTHQAQLASHKLYA
                     TGTTGHLLARETGLEITSLLSGPMGGDQQLGGLIAEKKIDLLVFFWDPMNAAPHDPDV
                     KALMRIATVWNIPVAINETTANFLLNADLFHQEVSVNVPDYDGYLAERLG"
     gene            2890..4164
                     /gene="thrC"
                     /locus_tag="PWX02_001544"
     CDS             2890..4164
                     /gene="thrC"
                     /locus_tag="PWX02_001544"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDL1729542.1"
                     /translation="MNLYNIKHPEEQVNFAQAVRQGLGKNQGLFFPEVLPKLGSIDEL
                     LDLPLVERSQKILSALIGEELPQETLNAMVKNAFTFPAPLAKVNDDIYALELFHGPTL
                     AFKDFGGRFMAQALANVRGDGKITILTATSGDTGAAVAHAFYGLENINVVILYPKGKI
                     SPLQEKLFCTLGGNIRTVAIESDFDACQALVKQAFDDAELRQAIGLNSANSINISRLL
                     AQICYYFEAVAQLPKEKRDNVVVSVPSGNFGNLTAGLIAKTLGLPIKRFIASTNANDT
                     VPRYLQSGKWEPNATVATLSNAMDVSRPNNWPRVEELFKRNGWSLSELGSAALNDAET
                     EAALKAQYAEGYLCEPHGAIAYQSLKDQLQQGETGIFLCTAHPAKFKESVERILAIEL
                     PLPEALDKHNKLPLLSDEMAADFATLRAYLLK"
     gene            complement(4243..4977)
                     /locus_tag="PWX02_001545"
     CDS             complement(4243..4977)
                     /locus_tag="PWX02_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDL1729543.1"
                     /translation="MSFKDMTGQKVPSVTFRTRQNDAWVDVTTDELFNNKTVVVFSLP
                     GAFTPTCSSTHLPRYNELACEFKALGVDDIICMSVNDTFVMNAWKADQESENITVIPD
                     GNGEFTEGMGLLVDKNDLGFGKRSWRYSMLVKNGIVEKMFIEPQEPGDPFKVSDADTM
                     IKYLSPNWTAKESVSIITKPGCPFCAKAKALLKEQGYTFEEIVLGRDASTISVRAITG
                     KTSVPQVFIGGKHIGGSDELAAYFAK"
     gene            5092..5976
                     /gene="oxyR"
                     /locus_tag="PWX02_001546"
     CDS             5092..5976
                     /gene="oxyR"
                     /locus_tag="PWX02_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HDL1729544.1"
                     /translation="MNIRDLEYLIALAEHKHFRRAAESCNVSQPTLSGQIRKLEDELG
                     TILLERTSRKVLFTQAGLTLVEQAKVVLREVKILKEMASNQGKEMSGPLHVGVIPTLG
                     PYISPIIVPALKAEFADLELYIYELQTNQLLDQLESGQLDCGIISFVKESESFIEVPI
                     FNEEMYLVVPEQHEWAALKKFDMNLLKNRELLFLDSGHCLRTNTLDYCLSIGAKENPH
                     FKATNLETLRNMVAANVGLALIPKLAAKPIKGVHYIPLENPKPFRAVGMVYRPGSPLR
                     ARYERLAQAITKIMQSEA"
     gene            5979..6611
                     /gene="fabR"
                     /locus_tag="PWX02_001547"
     CDS             5979..6611
                     /gene="fabR"
                     /locus_tag="PWX02_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HDL1729545.1"
                     /translation="MANGMSVRAIQKEKTRRALVEAAFNQLSSEKNFSNLSLREVARE
                     AGVAPTSFYRHFKDMDELGLAMVDESGLLLRQLMRQARKRIANGGSVVVVSVETFFEL
                     IADRPNVFQLLLRESSGTSQAFRTAASREIQHFVAELADYIMSKDNHSNRELAYIQSE
                     GLVTLVFTAGSHALDMNNQEREKLKQRVIMQLRMLIRGSTYYANKQASST"
     gene            complement(6638..6937)
                     /locus_tag="PWX02_001548"
     CDS             complement(6638..6937)
                     /locus_tag="PWX02_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HDL1729546.1"
                     /translation="MKKADIQKMQDCYNQWVELLPELEKGIEQWKRASALLEPLSRFY
                     ASPEWRELHDSFDEQLDTKGNYSILSEDALWNALSEHHQLALEWLKLSTAYITKE"
     gene            complement(7026..>9543)
                     /locus_tag="PWX02_001549"
     CDS             complement(7026..>9543)
                     /locus_tag="PWX02_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDL1729547.1"
                     /translation="NVAAGDVNADSKDAVNGSQLYTFAMASREEVKSTDKSVTVNTTK
                     NADGANVFDLSVNTDDVTIVKDPTTGAIKANTTALNDANNDGRIDEPTADDAKKLVTA
                     GDITNAINNSGFTLKTSAVEGGEKLSGGDELINPGKAVEMVAGKNLTVKQEADGKVIY
                     ATKDDVKFSSVTSNTVTVPTDEADPANNPITINKDGINAGNKAISNVASNLTPVTADD
                     KVQPADNNPTNLADKLSNAATLGDVLNAGWNLQGNGKAVDTVVHNDTVDFINGKGTTV
                     TVENKDGKNTIKVDSPIEFVNQDPTDSSTPSNTAKFTGEAPVQLGNVASSVRNEDGST
                     PEGKDRAEAIKNAEGDKLNNVVNLGDLQAATNAATTKVGGNRGVTITPSTNADGSTTY
                     NVEAKTDGTTIKVDNEGNITANTSELGNNEDGTVKAPTQPNALVTAQTVADAVNNAGF
                     NIKSAGNKAAGDQAATKLVKTGEEVVFEAGDNLTVKRDGNQFTFATAKDVSFNSVQFS
                     ENGPKITNDGDNIKVGDKDGKPTKITNVADGDISPVSTDVINGKQLNNYAKVNGNNIG
                     TDEDGSINIVNGNGTTITSDKAGEVKVNVNSTDLTVADNGKINVQDPNGTGSHFVNAT
                     TVANAVNNVSWNVDSKAVGTGVVEGDKAPAKVKAGSTVSVNAGNNIKVTRKGSDVTVA
                     VSDTPEFTSVKTGDTLVNNNGVTINNGSAGKAVSLTKDGLNNGGNRITNVKAGEADTD
                     AVNVGQLKGAVNHLNNKIHRNNREARAGIAGSNAAALPQVYIPGKSMVAAAGGTFKGE
                     NALAVGYSRSSDNGKLILKLQGNANSRGDFGGGVGVGYQW"
BASE COUNT         2737 a         2183 c         1817 g         2806 t
ORIGIN      
        1 atcctactac aaattatata attaatacaa ccgtaggggc gggccctgtg cccgcccgtg
       61 cttcttctat tactgatggc aaatttcagc tttcagcaag ctttgcatct gcggtaacaa
      121 ctgcacaatc agcctcagtt gttgtatcaa cactaaggaa agatcatttt gtttttgctg
      181 attttcctgc tcaaactggg ctaggtcttg gtcgatagcg gtcaattttt ccgataattt
      241 tgcaaatgaa cggctctctg aggcgatttg cccaatgatg tttgccacct ctctggcgag
      301 tgagaagaac attgcggaaa aatccacttg ttgattaatc tctcggctag caatacggta
      361 agcccctaag gtggaaatat aacccagcaa ggtgtaagca atacttaaca atttcggggc
      421 gaaggttaaa tcgttcgcat atttttgtgg ttcgctgtac atattggtaa ttgcattact
      481 gagtgccgat aaatggttat gcacatcacg gcgtgcaatg cggtaaggta gttgctcgtt
      541 ataaccaaac tgcaactggg caataatatg gcgtaaatat tgtccactag ctttcaagct
      601 attatgtaag ttgcgttgca aatttaaata tttccaatcc ggccataaga atgctaccgc
      661 caaccacgca atcgcagtgc cgagcaaagt atcgagcatt cgcattaaga tgccgctttc
      721 aaggctcacg cctgcaatat caaggcttat cattaccaac accgtaatat aaaaggttga
      781 aaagccataa tgtaccaagc ggaaaaaggt atagagcgag ccggtcagta cgattagccc
      841 aagctgggct tccagactga gcgttacgtt aaacaattca aaagagccac cgaccagcac
      901 gccgaaaatc gtgccgatta cacgctgaat cagtcgggtc ttagtggcgg tgtaattcgg
      961 ttggcagaca aaaatcgccg tgagcaaaat ccagtaacct ttgctgtcga agcctaaaat
     1021 cggcacgatc gcactgcaca acagcaccac tatcgacagc cgaacggcgt ggcgaaacag
     1081 ttgggattca aaggttaaat gggctttaat cgcttggctg atattgctta agccggtaat
     1141 attttcggcg atcaagcggt gagatttggt gaaatttttg cttaacggct caatatgctc
     1201 accactgctt gagccttgtt caatctgagc taattgccgt tcaatattgc ctaaattttc
     1261 tgcaatagca cgccaacgat aggcactttt caagcccctt tcttggtgat aatttaatga
     1321 gttaagtaac ccctgcaagg ctttttcgcc acacataccg tgttcatata cttccctatg
     1381 gcgtaacgac atcgcaattt tttgacacgc cgccgcctgt aattccaata cccgttgaaa
     1441 gcgaaacatt aaatcactgt tgcttaattg ttgaaacagc tcgtgatatt gataatggct
     1501 ggagcttgcc cgctctaaaa tatcttgagc ggtgaaatag taacgcagca ttcgctgtgt
     1561 ccgttgttgc ttgtgctgac gtcttaggcg gtagaatagg gcgactctgg ttttatcaaa
     1621 agcagccatc acctcgctat tggctttcgc caaagctaac tgtttcgcag ctaaatcgtc
     1681 atcatcgggg tcaaaatagg cagatttcgc cagtaaatag tggctcaatg caaggtaggc
     1741 ggacgctaaa ttctcctgta cagtgcgatt cggaaaaatc aggtaaacca aaataccgac
     1801 caaaccatag agtaaggtgc caagtaagat catcaccaca ttgtgatacc acaatacttc
     1861 gggcgtataa gccaggctgt tataaaccgc cacaatcagc gtgccaaaag caatggtact
     1921 atagcgttgc ccgattgccc ctaacattac cagcaaaaag gtgcaaacca ccgccaccgg
     1981 cacaaacagc cagcccaaat ggagcgacac actcgcccca atcgaggaaa gcgagaacgc
     2041 cactaggctg ataatcagat ttttcgctcg tccgattagg ctgttgtcta aatccaccaa
     2101 accgccggcg ataatgccta acatcaacgg catcgcaaaa tcagaaatac cgagttgcca
     2161 aatactaacc gctgcaatat tcagcgaaat aaacataggc agcgttttaa ttacattttc
     2221 cgaaagccaa ctgtgttgta gatatcttaa aaatttattc atatttgctc atttcgggca
     2281 ggcacagaac ctgcccctac ctacactacc ctaaacgttc tgctaaatag ccatcataat
     2341 ccggcacatt cacgctgact tcttgatgaa acaaatccgc atttaacaga aaattagcgg
     2401 tagtttcgtt aatcgccacc ggaatattcc aaacggtcgc aatccgcatc agggctttca
     2461 catcaggatc gtgcggagcg gcattcatcg gatcccaaaa gaacaccagc agatcaattt
     2521 tcttttctgc gatcagcccg cctaattgct gatcgccccc catcgggccg cttaacaatg
     2581 aagtaatttc aagccctgtt tctctggcga gcaaatgccc ggtagtgccg gtggcatata
     2641 gcttatgcga ggcaagttgg gcttgatgag tttgtgtcca acgaattaaa tcttgtttac
     2701 agctatcgtg agccactaag gcaatctgtt tttgttttgc gatggttctg acggtggttt
     2761 tcatttttag ctcctttgca aaatttttca caaatcatac cgcttatttt tcgcttttgc
     2821 attgactaat cacggcttat ggctaaaatc aggtaaaaat tcgcatttta aaacaaagga
     2881 ttaaccgcaa tgaacttata caacatcaaa caccctgaag aacaagtcaa tttcgcccaa
     2941 gcggtacgcc aaggcttagg caaaaatcaa gggctatttt tccctgaagt gttacctaaa
     3001 ttaggtagca ttgatgaatt attggattta cctttggttg aacgcagcca aaaaattctt
     3061 tcagccttaa tcggcgaaga gttaccgcaa gaaaccttaa atgcaatggt aaaaaatgcg
     3121 ttcactttcc cggcaccgct tgccaaagtc aatgatgata tttatgcgtt agaattattt
     3181 cacggtccga ctttagcctt caaagatttt ggtgggcgct ttatggctca agccttagcc
     3241 aatgtacgag gagacggcaa aattactatt ttaaccgcta cctcaggtga caccggtgcg
     3301 gcggtggctc atgctttcta tggtttggaa aatatcaacg tggtcattct ttacccgaaa
     3361 ggcaaaatca gcccattaca agaaaaatta ttctgtacct taggcggtaa tattcgcact
     3421 gttgcgattg aatccgactt tgatgcttgc caagccttag tgaaacaagc ctttgatgat
     3481 gccgagctac gccaagcgat tggtttaaat tccgcaaatt ccatcaacat tagccgttta
     3541 ctggctcaaa tttgctacta ctttgaagca gttgctcagt taccaaaaga aaaacgagat
     3601 aacgtagtgg tttcagtacc aagcggtaat ttcggtaact taaccgccgg cttaattgcc
     3661 aaaacattag gtttaccgat taaacgtttt attgcctcaa ccaatgccaa tgacaccgtg
     3721 ccacgctatt tacaatcagg caaatgggaa ccaaacgcaa ccgttgcaac cctttctaat
     3781 gcaatggacg taagccgccc gaacaactgg ccacgagttg aagaattatt taaacgcaat
     3841 ggctggagtt tgtctgaatt gggttctgcc gcattaaacg atgccgaaac cgaagcggct
     3901 ttgaaagctc aatatgcaga aggttatctg tgtgagcctc acggtgctat tgcttaccaa
     3961 agcttgaaag atcagctaca acaaggcgaa accggtattt tcttatgcac cgctcacccg
     4021 gcaaaattca aagagtcggt ggaacgcatt ttagcgattg aattgccgtt accggaagca
     4081 ttagataagc acaacaaact gccactgtta tccgatgaaa tggcagcaga ttttgcaaca
     4141 ttgcgtgcat atttgttgaa ataaatattc aataatagaa aagggattaa gtattaaact
     4201 taatccctct ttttatgcat tacaagcggt tatatttgcc tattattttg caaaataagc
     4261 agctaactca tcactaccgc caatatgttt accgccgata aatacttgtg gtacagaggt
     4321 tttacctgta attgctcgta cagaaatcgt gcttgcatca cgacctaaca caatctcctc
     4381 aaaagtatag ccttgctctt ttaataatgc ttttgctttt gcacagaacg ggcaacccgg
     4441 tttagtgata atcgaaacag actctttagc tgtccaattt ggacttaaat atttaatcat
     4501 tgtatcggca tctgatactt taaacggatc gcccggttct tgtggctcaa taaacatttt
     4561 ttccacaata ccgtttttca ccagcatcga ataacgccaa gaacgtttac caaacccaag
     4621 gtcattttta tcaactaata gccccatacc ttctgtgaac tcaccattac catcaggaat
     4681 aacagtaata ttttcagatt cttgatctgc tttccaagcg ttcattacaa aggtatcatt
     4741 aactgacata cagatgatat catctacccc taatgcctta aattcacaag ctaattcatt
     4801 atagcgaggc aaatgtgttg atgaacaagt cggtgtaaat gctccgggta atgaaaacac
     4861 aaccaccgtt ttgttattaa ataactcatc ggttgtgaca tcaacccaag catcgttttg
     4921 acgtgtacga aatgttacac tgggtacttt ctgacccgtc atatctttaa aagacatatt
     4981 aatgttctcc tatagaatta tatattataa aaatcttagg cattatactc acaaatatgg
     5041 cataattcag cctatcggaa caataggaaa aatttaaatt ggaggaatca agtgaatatt
     5101 cgagatttag aataccttat tgcacttgca gaacataagc atttccgtcg tgcagcagaa
     5161 tcttgtaatg ttagccaacc tactttaagc gggcaaattc gcaaattaga agacgaacta
     5221 ggcactattc tgcttgaaag aaccagtcgc aaagtcctct ttacgcaagc aggcttaaca
     5281 ttagttgaac aagccaaggt agttttacgt gaagtaaaaa ttctaaaaga aatggcaagc
     5341 aaccaaggta aagagatgtc tggcccgctt catgttggtg taatcccaac cttggggcct
     5401 tacatttcgc caattattgt gcctgcatta aaagcagaat ttgcagattt agagctgtat
     5461 atttacgaat tacaaaccaa ccaattatta gatcaacttg aatccggcca gcttgattgt
     5521 ggcattatct catttgtaaa agagagtgaa tcttttattg aagtgcctat ctttaatgaa
     5581 gaaatgtact tggtagttcc agaacaacac gaatgggcag cgcttaaaaa attcgatatg
     5641 aatttattaa aaaaccgtga gttgctcttt ttagacagtg gccattgttt acgcactaat
     5701 acgctagact attgtctatc cattggagca aaagaaaatc cacactttaa agctaccaac
     5761 cttgaaacct tacgcaatat ggtagcagca aatgttggcc tagcattgat tcctaaatta
     5821 gccgctaaac cgattaaagg agtacattat attccacttg aaaacccgaa accgtttcga
     5881 gctgtgggaa tggtgtatcg accaggttca ccattacgcg cccgctatga gcgtttagct
     5941 caagccatta ctaaaattat gcaatccgag gcgtaaaaat ggcaaacggt atgagtgtgc
     6001 gcgcaattca gaaagaaaaa acacgcagag cattagtaga agccgccttt aatcaattaa
     6061 gctcagaaaa aaatttctct aatctcagtt tgcgtgaagt tgcgcgtgaa gctggagtcg
     6121 caccgacttc gttttaccgc cactttaaag atatggacga actaggctta gcaatggtgg
     6181 acgaatccgg gctactatta cgccaattaa tgaggcaagc tcgaaaacga attgcaaatg
     6241 gcggaagtgt tgtcgtggtt tcagtggaaa cattcttcga actgattgcc gaccgcccta
     6301 acgttttcca gctattacta cgcgaaagct ccggtacatc gcaagcgttt agaaccgcag
     6361 catcacgcga aattcaacat tttgtagctg aattggcgga ctatattatg agcaaagata
     6421 atcacagtaa ccgagaactt gcttatattc aatcggaagg tttagtcacg ctagtattta
     6481 ctgccggttc tcatgctttg gatatgaaca atcaagaacg ggaaaaacta aagcaacgtg
     6541 tcattatgca gctaagaatg ctgattcgtg gctccaccta ttatgccaat aaacaagctt
     6601 catcaacata aaaaaacaga cgcctaagcg tctgttttta ttctttcgta atatatgcag
     6661 tcgatagctt taaccattct agtgctaatt gatgatgttc agataacgca ttccataacg
     6721 catcttctga caagatacta taattgcctt tagtatctaa ctgttcatca aaactatcat
     6781 gtaattcacg ccattccggg ctagcataaa accgacttaa gggttccaaa agtgcagaag
     6841 ctcgtttcca ttgctcaatc cccttttcca attctggtaa tagctctacc cattggttat
     6901 aacaatcctg cattttctga atatctgctt ttttcattct ttaccgctta caccataaat
     6961 tttagcctca atataacata aaaatcccct tccgaagaag gggatttcaa ctcaattagg
     7021 ctaaattacc actggtatcc aacgcctaca ccgccaccaa agtcgcctcg gctattcgca
     7081 ttaccttgta atttcaagat aagcttaccg ttatcacttg aacgtgaata acctactgca
     7141 agcgcgtttt cacctttaaa tgtaccacct gctgcagcca ccatagattt acctggaatg
     7201 taaacctgcg gtaacgctgc tgcatttgaa ccggcaatac cagcacgggc ttcacggtta
     7261 ttacgatgga tcttgttatt taagtgatta acagcacctt ttaactgacc aacattaaca
     7321 gcatcggtat ctgcctcacc tgctttaaca ttagtaatac ggttaccacc attatttaag
     7381 ccatctttcg ttaagcttac cgcttttcct gcagagccat tattaatggt tacgccatta
     7441 ttgttcacca atgtatcacc agtcttaacg ctagtaaatt caggggtatc tgaaacggct
     7501 acagttacat cagaaccttt acgtgtcact ttgatattat tacctgcatt cacgctcact
     7561 gtactacctg cttttacttt cgcaggggct ttatcccctt caaccacacc tgtaccaact
     7621 gctttagaat ctacgttcca gcttacgttg tttacggcat ttgctactgt tgttgcattc
     7681 acaaagtgtg aaccggtacc gttaggatct tgaacattga ttttgccgtt atcagcaacg
     7741 gttaaatctg tgctgtttac gttcactttc acctcaccag ccttatcact ggtaatagtt
     7801 gtaccattac catttacgat attgatagaa ccatcttcat cagtaccaat gttattacca
     7861 ttcactttcg catagttgtt cagctgttta ccgttgataa catctgtgct tactgggcta
     7921 atatcgccat ctgctacatt agtgatctta gttggtttac cgtctttatc accgactttg
     7981 atgttatcac catcattagt gattttcgga ccattctcac tgaactgtac gctattgaag
     8041 ctcacatctt ttgctgtcgc aaaagtaaat tgattaccat cacgtttaac agttaagtta
     8101 tcacctgctt caaacacaac ttcttcgccc gttttcacta gcttagtcgc agcttggtca
     8161 ccggcagcct tattacctgc agatttaata ttaaagcctg cattattcac tgcatctgca
     8221 acagtttgag cagtcactaa cgcatttggc tgagttggtg ctttcaccgt accatcttcg
     8281 ttgttaccta actcactggt atttgcagtg atgttacctt cattatctac tttaatagta
     8341 gtaccgtcag ttttcgcttc aacgttataa gtagttgaac catcagcatt cgtgctaggt
     8401 gtgatagtta cacctcggtt accgcccact ttcgtagtag ctgcattagt tgccgcttgt
     8461 aaatcaccta agttcactac gttgtttagc ttgtcgcctt cagcattttt aatcgcttct
     8521 gcacggtctt taccctctgg ggttgagcca tcttcgttac gaacactact tgccacattg
     8581 cctaattgaa caggggcttc acctgtgaat ttagccgtat tgcttggcgt tgagctatca
     8641 gtaggatctt ggtttacaaa ctcaatcggg ctatctactt taatcgtatt cttaccgtct
     8701 ttgttttcaa ccgtgaccgt tgtaccttta ccgttaataa aatcaaccgt gtcattatgt
     8761 accacagtat caaccgcttt gccgttacct tgtaaattcc agcctgcatt taatacatca
     8821 cctaatgttg ctgcattact taacttatca gctaagttgg ttggattatt gtctgctggt
     8881 tgaaccttat catctgcagt cacaggtgtc aggttagacg caacattgct aatcgcttta
     8941 ttacctgcat tgataccatc tttattgata gtaattggat tatttgcagg atctgcttca
     9001 tcagttggta cagttacagt gttagaggtt acgctgctaa acttaacatc atctttagtc
     9061 gcataaatga ctttaccatc agcttcttgc ttcacagtta agtttttacc tgcaaccatt
     9121 tcaactgctt tacccggatt aatcagttca tcaccacctg aaagcttctc tccaccttct
     9181 acagccgacg ttttcaacgt aaagcctgag ttattgatgg cgttggtaat atcacctgct
     9241 gtcactaatt tcttagcatc atcagcggtt ggttcatcaa ttctaccgtc attatttgca
     9301 tcatttaatg ccgtagtgtt agccttgatc gcaccggttg taggatcttt tacaattgtt
     9361 acatcgtccg tattcacaga aagatcgaac acattcgcgc catcagcatt ttttgtcgtg
     9421 ttaaccgtaa ctgatttatc agtcgacttc acttcttcac gagatgccat agcgaaagta
     9481 taaagctgac taccatttac tgcatcttta ctatctgcat ttacgtcacc ggcagctaca
     9541 tta
//
LOCUS       DANJWC010000090         9506 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.090, whole genome shotgun sequence.
ACCESSION   DANJWC010000090 DANJWC010000000
VERSION     DANJWC010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9506
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.090"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(180..1505)
                     /locus_tag="PWX02_001550"
     CDS             complement(180..1505)
                     /locus_tag="PWX02_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase
                     family protein"
                     /protein_id="HDL1729548.1"
                     /translation="MISSVWNERYLADKKREQDHRSPFQRDRARLLHSEAFRCLQAKT
                     QIHAVGEDDFYRTRLTHSLEVAQIGSSLREKLLADVEGFQAVCSEQEFAKNMANLTAL
                     LPDHSLVESLCFAHDLGHPPFGHGGEMALNYKMYAFGGFEGNAQSFRIMTQLEPYTET
                     AGMNLTRRTLLGVMKYPALLSQTSPIEPAEMSEARHINLHHFPQSKGIFDDDNHFFEW
                     VLAPLSEKDRRQFCTVQPNQDPKQPNKTQYKSLDCSIMELADDIAYGVHDLEDAIVGG
                     MVTPQSWQNAEKLLAECQSDWVKQRLPEIREKLFSQHRYERKDVIGALVNHFITNVRW
                     KALPEFDEPLLRYNAYLPESVACVLKILKDFVYQYVICDAKTQRVERKGQRILMELFD
                     ILSSDPMRLLPNNVRERWKNAEESKRARVICDYLAGMSDGQAFKLFESL"
     gene            1590..2909
                     /gene="rlmD"
                     /locus_tag="PWX02_001551"
     CDS             1590..2909
                     /gene="rlmD"
                     /locus_tag="PWX02_001551"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL1729549.1"
                     /translation="MAIFYSEKSAKKPIKSPACQKATIQSLDYQGLGIAKINGKTWFI
                     ENALPDEQVEFKVLEEKRQYGRGQAVKILKKSANRRMPSCELYGQCGGCQMQHISLEL
                     QRKAKQQALFQRLQKLQTHAIDVQPMIIGNDKHYRRRAKLSIAIQQGKAVIGFRLQNS
                     NQIIPLAHCEVLVEPLANLLPKLKHLIENWQQPKKLGHIELVSADNTISLFFRHLGAL
                     SAQDNHNLRQFATQEQLSLFVMTSENHIEHLCGERPYYQIQGLKLHFSIRDFIQVNAT
                     LNEKMVEKALEWLELSNQDRVLDLFCGMGNFTLPIAERAKSVVGVEGVEPMVQQAREN
                     AVTSGLKNVEFYQTNLDEPFSDKPWAKEPFNKVLLDPARNGALFCLDHLTSLNPERIV
                     YVSCNPATLVRDAEKLLQLGYRLEKAAMIDMFPHTGHLESISLFLKS"
     gene            complement(3049..4230)
                     /locus_tag="PWX02_001552"
     CDS             complement(3049..4230)
                     /locus_tag="PWX02_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HDL1729550.1"
                     /translation="MESRRPSLSFFIILGMMAMLPPLAIDMYLPSFLDIARDLAVSQE
                     KVQTTLAVFTLGFGAGQLFWGPMADSFGRKIIILIGLVGSAIAAYFLTQVQNIETFYL
                     LRLVQGLCAAAPAVVLGALVRDLFERNLFAKLMSVIMIISMLAPLLAPIVGGYIAKYF
                     HWHSIFYVLVAMGILCVVLISWKIPETLAPEKRLPLSFGKTLSNFAILLKHKPTLGYV
                     LIGGLTFAGIFCFLTSGSIVYIGLYGVSQENFGYFFGLNMIVMVTMTAINSKMVVKVG
                     SETMLRIALAMQLLAGIWLALVAIFDLGFWAMAIGIPLYIGMLSTIGSNASAAILEQF
                     PQMAGTANGVAGTARFGVASLVGAGLSHIAVTSAAPMLLAMALCVMLAGVVYYFLCYK
                     R"
     gene            complement(4220..4921)
                     /gene="rsuA"
                     /locus_tag="PWX02_001553"
     CDS             complement(4220..4921)
                     /gene="rsuA"
                     /locus_tag="PWX02_001553"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HDL1729551.1"
                     /translation="MRLDKFIAENTGLTRSQAAKVLKSGVVTVNGNIEKSGAAKISQE
                     DEIYYEDQKLEWVEEGQYFMLYKPQGYICSHDDGEYPTVFQFFDYPLMTKLHTAGRLD
                     VDTTGLVLLTDDGKWSHRITSPKHHCEKTYLVTLADLVEDFYAQRLAEGILLRGEKEP
                     TLPAQLEILDDYNVNLTISEGRYHQVKRMFAALGNKVEALHRWRIGDVVLDERLEEGE
                     FRSLTSEEIASFNGK"
     gene            complement(5166..6137)
                     /gene="menC"
                     /locus_tag="PWX02_001554"
     CDS             complement(5166..6137)
                     /gene="menC"
                     /locus_tag="PWX02_001554"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDL1729552.1"
                     /translation="MRQVKLYRYQLPIQTGVVLRKQPLKERQGVLVCLQEQEKVGWGE
                     IAPLPSFSQESLEQAENQAVNWLKAWQKGEPETLEQLFPSVAFGLSCALAELNNELGE
                     QANNQSAVLCYGDLEKLHTAFSQHTNPIGKIKIGLNPEQEGEQANLLLHTFPNLRLRL
                     DANRLWSLEQAVKFAEKIANEHKPRIQFIEEPCQALELSRQFATQTDIAIAWDETVRE
                     ANFLVKKEPNLTAIIIKPTLVGSLEKCVNLIQQAQKQGLVAVISSSLESSLGLTQLAR
                     IAQQYTPNSVPGLDTLNLMQHQLLRDWHDSSLPLVGLESEFVREVVV"
     gene            6349..8427
                     /gene="metG"
                     /locus_tag="PWX02_001555"
     CDS             6349..8427
                     /gene="metG"
                     /locus_tag="PWX02_001555"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDL1729553.1"
                     /translation="MANQKRKMLVTCALPYANGAIHLGHMLEHIQADIWVRFQRMRGN
                     EVHFVCADDAHGTPIMLNADKLGITPEQLIEKAKADHEADFASFNISFDNYHSTHSEE
                     NRQITTDIYKTLKANGFIKSKVISQLFDPEKNMFLPDRFVKGTCPKCKAEDQYGDNCE
                     VCASTYSPMDLINPRSAVSGATPVVKESEHFFFDLPSFEGMLKEWTRSGSLQPEIANK
                     MQEWFESGLQQWDISRDAPYFGFEIPDAENKFFYVWLDAPIGYMASFKNLCDRAGIDF
                     DEFWQKDSTTELYHFIGKDIVYFHSLFWPAMLEGSGYRKPTNVFAHGYVTVDGAKMSK
                     SRGTFIQANTYLKHLDPECLRYYYAAKLNDRIEDLDFNLDDFVQRVNSDIVNKLVNLA
                     SRNASFIAKRFEGKLAEKLDDEALFNEFTAQAETIANFYENREFNKAIRAIMELTDKA
                     NKYIDDKAPWVIAKEEGKEAELQAVCSMGIELFRVLMSYLKPVLPKLAERAEAFLQSE
                     LRWDNIATPLFGHTVAPFKSLFSRLEKKQIDAVIDETKALFAEANKKTENQTKGTQKV
                     EQQAVEKQENIAVEPISPEITIDDFAKLDLRAAKVLNCEAVPKSDKMLRFELDLGDHQ
                     RQVFSGIKAAYPNPEALIGRFVIVIANLAPRKMSFGMSEGMILSAGTGGANLFLLDAD
                     SGVQPGMQVK"
     gene            8437..8853
                     /gene="ndk"
                     /locus_tag="PWX02_001556"
     CDS             8437..8853
                     /gene="ndk"
                     /locus_tag="PWX02_001556"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDL1729554.1"
                     /translation="MIQQTLSIIKPDATRRNLIGKILAQLEENGLTIKALKKVHLTRE
                     QAEGFYAEHKGKDFFEPLVEFMTSGSIVVSVLEGENAINHYRALMGATNPDNREKGTI
                     RDLYALSMRENSVHGSDSEESAKREIAYFFVPSEIV"
     gene            complement(8910..9386)
                     /gene="greA"
                     /locus_tag="PWX02_001557"
     CDS             complement(8910..9386)
                     /gene="greA"
                     /locus_tag="PWX02_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDL1729555.1"
                     /translation="MKQIPMTVRGAEQLREELDFLKNVRRPQIIDAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIQEIEGKLGNAQIIDVTKMANNGKVIFGATVQLVNADTDEE
                     VTYRIAGDDEANIKEGLISVNSPIARGLIGKEVNDSVSITTPGGKVEFDIVGVEYI"
BASE COUNT         2793 a         2213 c         1915 g         2585 t
ORIGIN      
        1 cgatctgctt taactaattc gccacgaggc tgataaattt cacttggaag aaaccgcaaa
       61 tgctgttgca gaagccaaag ttgctaatac taaaagacct ttagaaacag ttttcattga
      121 aatctcctat taaataaatg aaacctatta ctgagacctt gcaaaattaa caaagttcat
      181 tataaacttt caaaaagttt aaacgcctgc ccatcagaca taccggctaa atagtcgcaa
      241 attacccttg cacgcttaga ttcttccgca tttttccaac gttcccgcac attattaggg
      301 agtaatcgca tcggatcact gcttaaaata tcgaacaatt ccattagaat gcgttgcccc
      361 ttgcgttcca cccgctgggt tttggcatca caaatcacat attgatacac aaaatctttc
      421 aagattttca gcacacaggc aacactttcc ggtaaatagg cattataacg tagcaacggc
      481 tcgtcaaatt caggcagagc tttccaacgc acattggtaa taaaatgatt cactaatgcc
      541 ccgatcacat cttttcgctc gtagcggtgc tgagaaaaga gcttttcccg aatctccggc
      601 aggcgttgtt tcacccaatc ggattggcac tccgccagca atttttccgc attttgccac
      661 gattgcggtg tgaccattcc acctacaatc gcatcttcca aatcgtgtac gccataggca
      721 atatcgtccg ctagttccat tatgctgcaa tcgagagatt tatattgcgt tttgtttggc
      781 tgtttcggat cttgattggg ctgaaccgtg caaaattggc gtcggtcttt ttcacttaac
      841 ggggctaata cccactcaaa aaagtggttg tcgtcatcaa aaatcccttt gctctgtgga
      901 aaatggtgca aattaatatg ccgagcctcg ctcatttctg ccggctcaat cggcgaggtt
      961 tgagacaaga gtgccggata tttcattacc cccagtaagg tgcgacgggt cagattcatt
     1021 ccggctgttt cggtgtaagg ctcaagctgg gtcataatcc gaaaggattg agcattaccc
     1081 tcgaagccgc caaaggcgta cattttataa tttaacgcca tttcaccacc gtgcccgaac
     1141 ggagggtgcc cgagatcgtg ggcaaaacaa agggattcca ccaagctatg atccggcaat
     1201 aaagcggtca gatttgccat attttttgca aattcctgct ccgaacagac cgcttgaaag
     1261 ccttccacat ccgctaataa tttttctcgc aaactgctac cgatttgagc cacttccaac
     1321 gaatgggtta aacgggttcg ataaaaatca tcttccccca cggcgtgaat ttgcgttttc
     1381 gcctgcaaac agcgaaaagc ctccgaatgc aataaccttg cccgatcccg ctgaaaaggc
     1441 gagcggtgat cttgttcccg ttttttatcc gccaaataac gttcattcca aaccgatgaa
     1501 atcatattgc ctccattttc cgccaactgc ttttcttata taatctcccc atttcgacac
     1561 tttatatcat aacaattcat ctgaaaacaa tggcgatttt ttattctgaa aaatcggcaa
     1621 aaaaaccgat aaaatcaccc gcttgtcaaa aggcaactat tcaatcgtta gattatcaag
     1681 gcttagggat tgccaaaatc aacggcaaaa cttggtttat cgaaaatgcc ctgcctgatg
     1741 agcaggtgga atttaaagtg ttggaagaga aacgccaata cggacgtgga caagcggtta
     1801 aaattcttaa aaaatctgca aatcggcgaa tgccaagctg cgaactttac gggcaatgcg
     1861 gtggctgcca aatgcagcat atttcgcttg aactgcaacg taaggcaaaa caacaggctt
     1921 tattccaacg cttacagaaa ttgcaaaccc acgctattga tgttcagccg atgattatcg
     1981 gcaacgataa acactaccgc cgtcgggcaa aattaagcat tgcgatccag caaggcaagg
     2041 cggtaatcgg ctttcgtttg caaaattcca accaaattat accgcttgca cactgtgaag
     2101 tgctagttga accgcttgcc aatttgctcc caaaattaaa acacctgatt gaaaactggc
     2161 aacagccgaa aaaattgggg catattgagc tggtcagtgc ggacaatacc atttccctat
     2221 ttttccgcca tttaggggca ttatcggcgc aagataacca caatttgcgt caattcgcca
     2281 cacaagagca attatctctt tttgtgatga ctagcgaaaa tcacattgag catctgtgcg
     2341 gtgaacgccc gtattatcaa attcagggtc taaagttgca tttttcaatt cgggatttta
     2401 ttcaggtgaa tgccacgctc aatgaaaaaa tggtggaaaa agcccttgag tggctggaat
     2461 tatccaacca agatcgagtg ttagatctct tctgtggaat ggggaatttt accttgccta
     2521 ttgctgagcg agcgaaatcg gtggtcggtg tggaaggggt agaaccaatg gtgcaacaag
     2581 cccgagagaa tgcggtgaca agcggtctaa aaaatgtgga attttaccaa accaatttag
     2641 atgagccgtt ttctgacaaa ccttgggcaa aagagccgtt taacaaggta ctactcgacc
     2701 cggcacgcaa cggagcacta ttttgcctag atcacttgac atcactcaat cctgagcgga
     2761 ttgtttatgt ctcttgcaat ccggcaacac tggtgcgaga tgccgaaaaa ctgttgcaac
     2821 taggctacag gctagaaaaa gcggcaatga ttgatatgtt cccccacacc gggcatttgg
     2881 aatctatttc gctgttttta aaatcttagt agatattgcc ccgatggtgc gagcgtccac
     2941 gctcgtacca cacaagcggt taattctgcc taaaaatttg caaaaaatgt actattatta
     3001 accgcttgta gaaatttgtt gggttttcgc aagctaaccc caacctagtt atcgcttata
     3061 acacagaaaa taatacacca caccggctaa cataacgcac aatgccatcg ccaaaagcat
     3121 tggggcagca ctggttacag caatatgtga aagccctgct ccaacaaggg aagcaacacc
     3181 aaaacgagcc gtacctgcta ctccgttagc cgtgcctgcc atttgtggaa attgctctaa
     3241 aatggcggca ctggcattac taccaatggt ggaaagcatt ccgatataaa gcggaatacc
     3301 gattgccatt gcccagaagc ctaaatcaaa aatcgccact aaagccagcc aaatgccggc
     3361 taacagttgc atagctaaag caattcgtaa catcgtttca gagccgactt tcactaccat
     3421 tttgctgtta atcgccgtca tcgtcaccat cacaatcata ttcagcccga agaaatagcc
     3481 gaaattttct tgcgacacgc cataaagccc aatatagaca atcgagcctg aggttaaaaa
     3541 acagaaaata cctgcaaagg ttaagccgcc aatcagcaca taaccaagag taggcttatg
     3601 tttcagtaag atggcaaaat tgcttaaagt tttgccaaaa cttaacggca ggcgtttttc
     3661 gggggctaat gtttcaggga ttttccaact aattaatacc acgcacaaaa tccccatcgc
     3721 cactaacaca tagaaaattg agtgccagtg gaaatatttg gcaatataac cgccaacaat
     3781 cggtgcaagc aatggggcaa gcatagaaat aatcataata accgacatca atttggcaaa
     3841 taaattacgc tcaaacagat ctctcactaa tgctcccagc accaccgccg gggctgccgc
     3901 acataagccc tgtactaacc ttagcaggta aaatgtttcg atattctgaa cttgggtcag
     3961 aaaataagcg gcaatcgccg agccgactaa tccgattaaa ataatgattt tccgcccaaa
     4021 gctgtctgcc atcggtcccc agaatagttg ccctgcccca aaacctaggg taaataccgc
     4081 caaggtggtt tgcacttttt cttgcgaaac cgccaagtca cgggcaatat ctaaaaatga
     4141 gggcaaatac atatcaatgg ctaatggtgg caacattgcc atcataccta atatgatgaa
     4201 aaagctcagg cttggtcttc tactttccat taaaactggc tatctcctct gaagtgagcg
     4261 agcggaattc accttcttcc aacctttcat ctaacaccac atcgccaatt cgccagcggt
     4321 gcaatgcctc gactttattc ccaagggcgg caaacattcg cttaacttga tgataacgcc
     4381 cctcactaat agttaaattc acattgtaat cgtctaaaat ttcaagttga gccggcaagg
     4441 ttggctcttt ctcgccacgc aataaaattc cttctgccaa tcgctgtgca taaaaatctt
     4501 ccaccagatc agcaagcgtc actaagtatg tcttttcaca atggtgcttg ggcgaggtaa
     4561 tacggtgcga ccatttgccg tcatcggtta acagcaccag ccctgtggta tccacatcta
     4621 accgccctgc ggtatgcaat ttagtcatta acggataatc aaagaattga aacaccgttg
     4681 ggtactcacc atcatcgtgt gaacaaatat agccctgtgg cttatagagc ataaaatact
     4741 gcccctcttc cacccactct aatttctgat cttcgtaata aatttcatct tcttgactga
     4801 tttttgccgc accacttttt tcaatattgc cgtttaccgt caccacacca cttttcagca
     4861 ctttagccgc ttgtgaacgg gtcaaaccgg tattttcggc gataaatttg tctaaacgca
     4921 tactattttt ataccataaa ttactttacc ctctcccctc gtgggagagg gacagatttt
     4981 tcttcgtcta gaagaaaaat cagggagagg ggggaaatac gtcttcgtcc agaagaaaaa
     5041 tcagggagag gggaaatgtg caaacttttt tagaaaaatg accgcttccc ctctctctgc
     5101 ttatttcgct gaacgctcat aagcggtctc tctcccacaa ggggagagag tgtttaattg
     5161 ggagattata ccacaacttc cctaacaaat tcgctttcta gcccgaccaa cggcaagcta
     5221 gagtcgtgcc aatctcgcaa taattggtgt tgcattaaat ttaacgtatc caaccccggc
     5281 acactgtttg gggtgtattg ttgggcgata cgagccagtt gggttaagcc caaactgctc
     5341 tcaagacttg aactaatcac cgctactaat ccttgcttct gtgcttgttg aattaaattc
     5401 acgcattttt caagcgaacc aaccagcgta ggtttaatga taatagcggt caaatttggc
     5461 tcttttttta ccaaaaaatt cgcctctctc acggtttcgt cccacgcaat cgcaatgtcg
     5521 gtttgtgtgg caaattggcg ggaaagttcg agcgcttggc aaggttcttc gataaattga
     5581 atacggggtt tatgttcatt tgcaattttt tcagcaaatt tgaccgcttg ttccaagctc
     5641 caaagacggt tggcatcaag ccttaggcgt aaattcggaa aagtatgcaa cagcaaattt
     5701 gcctgctcgc cttcctgttc aggattgaga ccgattttaa ttttaccgat tgggttcgta
     5761 tgttggctaa atgccgtgtg taacttttcc aaatcgccat agcacagcac cgccgattgg
     5821 ttattcgctt gctcgcctaa ttcattattc agttcagcca acgcacagct tagcccaaag
     5881 gcaacagacg ggaaaagctg ctctaacgtt tcaggctctc ctttctgcca cgcctttaac
     5941 caattcacag cttggttttc agcttgttct aggctctctt ggctaaaact tggaagcggt
     6001 gcaatttcgc cccagccgac tttctcctgt tcttgcaaac acaccaacac cccttgcctt
     6061 tctttcaaag gttgcttgcg taacactaca ccggtttgaa tggggagttg gtagcggtat
     6121 aactttactt gtcgcatact atttttatcc aaaataggtt tagaggcgaa acatctttcg
     6181 cccacatcat catttgggaa attgaggcga aaaatgtttc gcctctagcg catcatattc
     6241 taccgcttgt tagcccaaag ctctaataaa ttcacgccaa ctggtaaacc tgcaactttt
     6301 gggctaaaat agacggcaat ttttacccta attttaatga gaaaaagaat ggctaaccaa
     6361 aaacgaaaaa tgcttgttac ttgtgccttg ccctatgcta acggtgcaat tcatttaggt
     6421 catatgcttg aacatattca agcagatatt tgggtgcgtt ttcaacgtat gcgtggcaat
     6481 gaagtccact ttgtctgtgc tgatgatgcc cacggcacgc ctattatgct taatgccgat
     6541 aaattaggca ttacccctga acagttaatc gaaaaagcca aagccgatca cgaagcggat
     6601 tttgccagtt ttaatatcag ctttgataat taccattcca cccacagcga agagaaccgc
     6661 caaatcacta ccgatattta taaaacctta aaagcgaacg gttttattaa aagcaaagtg
     6721 atttcacaac tgttcgaccc agaaaaaaat atgttcttgc cggatcgctt tgtgaaaggg
     6781 acctgcccga aatgtaaagc cgaagatcaa tatggcgata actgtgaagt atgtgcctct
     6841 acctatagcc cgatggattt aatcaaccct cgctcggcag tttccggtgc aactccggtg
     6901 gtaaaagaat cagaacattt cttttttgac ctgccaagct ttgagggaat gttgaaagag
     6961 tggactcgtt ccggctcact ccaacctgaa attgccaata aaatgcaaga atggtttgaa
     7021 agtggtttac agcaatggga catcagccgt gatgcccctt atttcggttt tgaaattccg
     7081 gacgcagaaa acaaattctt ctacgtttgg ttagatgccc ctattggtta tatggcatcg
     7141 tttaaaaatt tatgcgaccg tgcaggcatt gattttgatg agttttggca aaaagattcc
     7201 accaccgagc tttatcactt catcggtaaa gacattgttt acttccacag cctgttttgg
     7261 ccggcaatgt tggaaggcag cggctaccgc aaaccgacca atgtgtttgc tcacggttat
     7321 gtgacagtgg acggggcgaa aatgtcgaaa tctcgtggta cattcattca agccaacacc
     7381 tatttaaaac atctcgatcc ggaatgctta cgctattact atgcggcaaa attaaacgat
     7441 cgcattgaag atttagattt taatttagac gacttcgtgc aacgtgtaaa cagcgatatc
     7501 gtaaacaaat tagtgaatct tgcttctcgc aacgccagtt ttatcgctaa acgctttgag
     7561 ggcaaattag ctgaaaaatt agatgacgaa gcgttattta atgaatttac cgctcaagca
     7621 gaaaccatag cgaatttcta tgaaaaccgt gaatttaata aagctattcg tgccattatg
     7681 gaattaaccg ataaagccaa taaatacatt gacgacaaag ccccttgggt catcgccaaa
     7741 gaagaaggca aagaggctga attacaagcg gtttgttcaa tgggcattga gttattccgt
     7801 gtgctaatga gctacctaaa accggtatta ccaaaactgg cagaacgtgc tgaagcattc
     7861 ttacaaagcg aattgcgttg ggataatatc gctaccccat tattcgggca taccgttgca
     7921 ccgtttaaat cgctgttctc acgccttgag aaaaaacaga ttgatgcagt tattgatgaa
     7981 accaaagcct tatttgcaga agcgaataaa aaaaccgaaa accaaaccaa aggaacacaa
     8041 aaagtggaac aacaagcagt cgaaaaacaa gaaaatattg cagttgagcc aatcagccct
     8101 gaaattacga ttgatgattt cgctaaatta gatttgcgtg cagccaaagt attgaactgc
     8161 gaggcagtgc caaaatcaga caaaatgtta cgttttgagc tggatttagg tgatcaccaa
     8221 cgccaagtgt tctccggcat taaagcggcc tacccaaatc cggaagcact tatcgggcgt
     8281 tttgtgattg taattgccaa ccttgctcca cgcaaaatgt cgttcggtat gtcggaagga
     8341 atgattttat ctgccggcac aggcggtgcg aacctgttct tattagatgc agacagcggt
     8401 gttcagccgg gaatgcaagt taaataggag aagatgatga ttcaacaaac gctttctatt
     8461 attaagccgg atgctacacg ccgtaattta atcggcaaaa ttttggctca attggaagaa
     8521 aacgggctga ctatcaaagc tctaaaaaaa gtacatctta cccgtgagca agcggaagga
     8581 ttttatgcgg aacacaaagg caaagatttc tttgagccgt tagtggaatt tatgacatca
     8641 ggatctattg tcgtttctgt actggaaggg gaaaatgcga tcaaccatta tcgtgcatta
     8701 atgggagcaa ccaatccgga taaccgtgaa aaaggcacaa ttcgtgatct ttacgcctta
     8761 agtatgcgtg aaaattcagt acacggctca gattcagaag agtcggcaaa acgtgaaatt
     8821 gcgtacttct ttgtgccgag tgagattgtt taataaagta ggggcgaaaa atttttcgcc
     8881 ccactattgt aattcgcccc tagcgtagat taaatatatt ccacacccac aatgtcaaac
     8941 tcgaccttac cgcccggtgt ggtaatgctc accgaatcgt tcacctcttt gccgattaaa
     9001 ccacgagcaa tcggggaatt aactgaaatt aagccctctt taatattggc ttcatcatca
     9061 cccgcaatgc ggtaagtgac ttcttcatct gtatcagcat tcactaactg caccgttgcc
     9121 ccaaaaatca ctttgccgtt gtttgccatt ttagtgacgt caataatttg agcattgcct
     9181 aatttgcctt caatttcttg aatacgcccc tcacagaaac cttgttgttc acgggctgcg
     9241 tgatactcgg cgttctcttt taaatcgccg tgttcacggg cttctgcaat cgcatcaata
     9301 atttgagggc gacgcacatt ttttagaaaa tccaattctt cacgcagttg ctctgcacca
     9361 cgcaccgtca taggaatttg tttcattaaa tctatttcct tactaaaaat tatcggttta
     9421 cgaattaaaa cgaaagggct attattcaat ttctcactaa aaatttcaac tcccctttta
     9481 ttatgatttt taaaaatttc actcgt
//
LOCUS       DANJWC010000091         9311 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.091, whole genome shotgun sequence.
ACCESSION   DANJWC010000091 DANJWC010000000
VERSION     DANJWC010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9311
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.091"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            186..1139
                     /gene="msrP"
                     /locus_tag="PWX02_001558"
     CDS             186..1139
                     /gene="msrP"
                     /locus_tag="PWX02_001558"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HDL1729556.1"
                     /translation="MKKLTIQDVTPETIFYQRRKIITALGLGIAASALPKVSMAETQR
                     HLQSLQFKPDPNKGLELTPENKVIGYNNFYEFGVDKSAPAKNAANFKTDPWTLEIGGE
                     VENPFSLNHQQLLNTFPLEERIYRFRCVEAWSMVVPWIGFELARLIEMAKPTSKAKYV
                     VFHTLYDPAQMPGQKNRLLGGGIDYPYVEALRLDEALNSLTLLSVGLYGKTLPPQNGA
                     PIRLVVPWKYGFKSIKSIVKITFAEKRPLTTWESLAPNEYGFYANVNPNVDHPRWSQA
                     SERVIGAGGLLSVKRQPTLMFNGYENEVANLYRGLDLKVNY"
     gene            1139..1723
                     /locus_tag="PWX02_001559"
     CDS             1139..1723
                     /locus_tag="PWX02_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoxide reductase heme-binding subunit YedZ"
                     /protein_id="HDL1729557.1"
                     /translation="MLTWLRILIHLGCLLPLVWVAQLLYTGNETILGADPIKELEHFL
                     GYGAIVIFCIMFLLGIALQYLKKNQYQILRRPLGLWAFVWAALHISSYLLLELSLDVK
                     LFFSELAGRPYLILGAAAFFILSIMAITSLPMLKRKLGTRWGTIHQWAYPALVLAMVH
                     YYWSVKSVTFDPIIIGVLVLFIVAHKIQAKFAKK"
     gene            1820..2471
                     /locus_tag="PWX02_001560"
                     /pseudo
     CDS             1820..2471
                     /locus_tag="PWX02_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021111752.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            2723..3913
                     /locus_tag="PWX02_001561"
     CDS             2723..3913
                     /locus_tag="PWX02_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HDL1729558.1"
                     /translation="MSTSQQLIQQTEHFGANNYLPLPICISKAKGIWVEDPEGNRYLD
                     MLSAYSAVNQGHCHPKIVQALKDQADKVTLTSRAFYNDQLGIWYEKICKLANKEMALP
                     MNTGAEAVETAIKTARRWGYDVKGIAENQAEIIACVGNFHGRTMAAVSLSSDPDYKRG
                     FGPMLGGIKLVPYGDLEALKAAITPNTAAFLVEPIQGEAGILIPPEGFLEQAYELCKE
                     NNVLFIADEIQAGLGRTGKLFATEWEGFEPDIYILGKALGGGVFPISCVLANRDILGV
                     FNPGSHGSTFGGNPLACAVSIASIDVLLEEKLAERSRELGEYFLAELKKIQNPIIKEV
                     RGRGLFIGLELHQPARPFCEKLKEKGLLCKETHDTVIRFAPPLVIEKFDLDWALEKIR
                     EVLA"
     gene            3988..5499
                     /gene="nhaC"
                     /locus_tag="PWX02_001562"
     CDS             3988..5499
                     /gene="nhaC"
                     /locus_tag="PWX02_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL1729559.1"
                     /translation="MLILQTFSKNQPLVKGGFMSNRTEITDQNIRIPFRYAILPVIIM
                     VSIMAVAIIKFEASPHVPLLIGAISAAVIAMAHGYRWETIEKGIYRGIRMALPAIVII
                     IMIGLTIGAWLGGGIVATMIYYGLKLISPSLFLVTISIICAIVTLAIGSSWSTMGTIG
                     IAAMGIGFSLGIPAPMVAGAVISGAYFGDKMSPLSDTTNLAAGITETNLFEHIRHMFI
                     TTVPAFLIALSVYFYFGMEFRAENLDLAKIEDIMQAMQEHFLISPWLLLVPLLVVVLV
                     AKKVPALPALAAGIILGWLCHVFVQGGDVSVAVITLHDGYKIESGHEMIDKLFNRGGI
                     ESMMYTISLTIVAMTFGGIAEQAGMLHSVVEKILTLAKSAGSLISATIVSAFITNVTT
                     SEQYISILLPGRMYIKAYRDKGLSSRNLSRALEDGGTVTSVLVPWNTCGVYAFSMLQV
                     SAFEYAPYAIMNWLIPLIAIGLATLNIKIERLPPEELAKEPEEITLPKRGCCP"
     gene            5616..9215
                     /gene="putA"
                     /locus_tag="PWX02_001563"
     CDS             5616..9215
                     /gene="putA"
                     /locus_tag="PWX02_001563"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HDL1729560.1"
                     /translation="MMSYQLLPNTTIRPELSRHYRFDEQTLVQNLIEQAHCDEQADNI
                     KKLTTQLVGKVRTARQKASGVDALMHEFSLSSQEGVALMCLAEALLRIPDTATADKLI
                     RDKIAKGDWRSHTGNSPSLFVNAAAWGLVITGKLVSTHSESSLSSALSRLIAKGGEPL
                     IRKGVDVAMRMLGKQFVTGETIQQAIKNGEKRFAMGYRYSYDMLGEAALTAEDAKRYY
                     NDYVASIHAVGATSKGLGVYKSSGVSVKLSAIHPRYSLSQHQRVMDELYPRLKELFLL
                     AKKYDIGLNIDAEEADRLELSLDLMDRLLADPDLAGFNGIGFVVQAYQKRCPYVIEHL
                     IETARKHNRQLMIRLVKGAYWDTEIKRAQMDGAEGYPVFSRKVHTDLNYIVCAKKLLA
                     AQDVIYPQFATHNAQTLATIYHLAQGKRFEFQCLHGMGESLYDQVVGSDNLNVQCRIY
                     APVGTYQTLLAYLVRRLLENGANSSFVSQIVDENLSVDDLAQDPVQKAELTNGTMHPK
                     IPLPVDLFDDNRVNSKGYDLTDAVSLQNLENALNELTKQHYRAEPQLANDNTSGQESR
                     KIYNPANQAELVGEVIDATENDMEQAFESAAAFQGEWANTSPAVRAKCLEKMADLMEA
                     NMPQLFDLAVREAGKTLNNAIAEVREAVDFCRYYAKEVRNNIEAYKNPRGIVVAISPW
                     NFPLAIFVGEVSSALVAGNVVLAKPAEQTSLMAHYAASLFYQAGVPKAALQCLPGRGK
                     TVGVALVADHRVNGVIFTGSTETAKNINRNLHKNEKIIPLIAETGGQNAMIVDSSALG
                     EQVVIDVLNSAFDSAGQRCSALRVLYLQRDVADHILTMLKGAMAELKVGLPQHLTTDV
                     GPVIDKVAQQRLLDHIENLKKSAKSYYQVAISSEIAENGIFVPPTLLEIDHISQLKHE
                     VFGPVLHVIRFDADKLDQVIADINSTGFGLTSGLHSRIDETVDLWLKSVHAGNLYVNR
                     NTVGAVVGVQPFGGMGLSGTGPKAGGPLYLQRLVNRQEWTLNGLQGNAQAVDFDLNFA
                     IRKVLSGAEQQTALDLAKSLARRSPLGKTYPMQGITGEKNEMIFVARPQIGIGKGDLA
                     SQVQALIAIVTVGSQAVVEKSSELAKHASAFGKKLQVVDDFNSLDHLSTLIVLDLLSS
                     EEKLHHAERKGAILTYIEGLDLALSLLPLVHEKAISINTAAAGGNASLMSEMD"
BASE COUNT         2706 a         1895 c         2127 g         2583 t
ORIGIN      
        1 tcggctagtt gtagggtggc aaatttaacc atttgctttc taatctcttc tatctgtgta
       61 gaagatagac tcttcatata tctcctttag ggaataccca ataaaaaatt acacttattt
      121 tgtaagaatt ctagatcagc tgagtccaaa ataataacaa caattcataa aaaaaggata
      181 gttcgatgaa aaaattaaca attcaagatg ttacacccga aactattttc taccaacgcc
      241 gtaaaattat taccgcgtta ggtttaggta ttgcagcttc tgcattgcct aaagtaagta
      301 tggcagaaac acaaagacac ttgcaatcac tacagtttaa accggatcca aataaagggc
      361 ttgaactcac tccggaaaac aaagtaatag gctataacaa tttctatgag tttggcgtgg
      421 ataaaagtgc accagcaaaa aatgcggcaa acttcaaaac cgatccttgg acattggaaa
      481 ttggcggaga agttgaaaat ccgtttagct taaatcatca acaattgctt aatacgtttc
      541 cactagaaga acgcatttac cgttttcgct gtgttgaagc gtggtcaatg gttgtgccgt
      601 ggatcggctt tgagttagct cgcttaattg agatggcaaa accaaccagt aaagccaaat
      661 atgtggtctt tcacacttta tatgatcctg cacaaatgcc ggggcaaaaa aatcgcttgc
      721 taggcggtgg gattgattat ccttatgtag aagctttacg cttagatgaa gccttaaatt
      781 cattaacact actatccgtc ggtttatacg gtaaaaccct gccaccgcaa aacggtgctc
      841 ctattcgctt agtcgtgcct tggaaatatg gctttaaaag tattaagtcg attgtgaaaa
      901 tcacctttgc tgaaaaacgc ccactcacta cttgggaaag tcttgctcca aatgaatatg
      961 gcttttatgc taatgtgaat ccaaatgttg atcatccacg ttggtcgcaa gcatctgaac
     1021 gagtaattgg tgcaggcggg ttattatccg tcaaacgcca accaacacta atgtttaatg
     1081 gctacgaaaa tgaagtggca aatttatacc gtggcttaga tttaaaggtg aactactaat
     1141 gctaacctgg ttacgtatac tcattcatct tggctgttta ttaccgctag tttgggtagc
     1201 acagctatta tatacgggta atgaaacaat attaggtgcc gatccgatta aagagctgga
     1261 gcattttctc ggttatggtg caatcgtcat tttttgtata atgtttctac ttggtattgc
     1321 cctacaatac ctaaagaaaa accagtatca aattttacgc cgtccattgg gcttatgggc
     1381 gtttgtatgg gcagctctac atatttcaag ctatctattg ttagaattaa gccttgatgt
     1441 aaaattattt tttagcgagc ttgccggtcg cccatatttg attttaggag cagctgcttt
     1501 tttcattcta agcataatgg caataacttc gctgcctatg ctaaaacgaa aattaggaac
     1561 acgctggggg acgattcacc agtgggctta tcctgcatta gtattggcga tggtacatta
     1621 ttattggtcg gtaaagagcg taacattcga tccaattatc atcggagtct tagtgctgtt
     1681 tattgttgct cataaaatac aggctaaatt tgcaaaaaaa tagcaaaatc acaccgcttg
     1741 cagcagtcaa actacataca aaaaataagg ggctgtccta gataacgact aaaacatcca
     1801 tttaggttaa gataacccaa tgagaaaaag tcgtctaagt cagtacaaac aacataaact
     1861 cattgaactg tttgtagcag gtgtcactgc tcgaacagcg gctgagttgg tgaatgtaaa
     1921 caaaaccacc gctgcctatt actttcaccg tttgcgattg ctcatctatc aacatagtcc
     1981 tcatctggaa atgtttgagg gtgaaattga agcggacgaa agttatttcg gtggtgctcg
     2041 caaaggtaaa cgagggtgtg gtgccgcagg caaaactgcc gtatttgggc tgttaaaacg
     2101 tgatgacaag gtttataccg ttgtcgtacc aaatacgcaa tctgccacac ttttgccgat
     2161 tattcgggag gaaagtgaag cctgatagcc ttgtttacac cgatttttat cgaagctatg
     2221 atgtgcttga tgtgagtgaa tttaaccatt ttcgtatcaa tcacagcaaa cacttcgcag
     2281 aaaatcaaaa tcacattaac ggaattgaga atttttggag tcaagcaaaa cgccatttgc
     2341 gtaaatttaa cggcatacct aaagctcatt ttgagctgta tttgaaggaa tgcgaatagc
     2401 gttttaatca cagtgactta aaatctcaaa tttccatttt aaaacaattg gttaggggtt
     2461 gtttaagtta gttatctagg acagccctaa attttatagt tggaatttca acggagaaag
     2521 ttatctataa acttataaaa gttatagaaa atggaggcat ttttgcttaa agtatgctat
     2581 ctattcatat taattgcata gttaatcaac aactgaagaa aaatgagaag tagatcataa
     2641 aatcaacaaa aagtccgtgt tttttagcta aagattgtta aactcttttg tgttacaagt
     2701 ttttaacata aaggagtttt ttatgtctac ttctcaacaa ctcatccaac aaaccgaaca
     2761 tttcggtgcc aataactacc tacctctacc tatatgtatt agtaaagcta aaggcatttg
     2821 ggttgaagat cctgagggta atcgctattt ggatatgctt agtgcctact cagcggttaa
     2881 tcaagggcat tgtcatccaa aaattgtaca ggcactaaaa gatcaagcag acaaagtaac
     2941 gctgacctct cgagcatttt ataacgatca acttggtatt tggtatgaaa aaatttgcaa
     3001 actcgctaat aaagagatgg cattaccgat gaatacgggt gcagaagcgg tagaaacagc
     3061 gattaaaact gctcgccgtt ggggttatga tgtgaaagga attgcagaaa atcaagcaga
     3121 aatcatcgct tgtgttggca attttcacgg acgtacaatg gctgcagtat ctctctcgtc
     3181 tgatccggat tataaacgtg gtttcgggcc gatgttaggt ggcatcaaac ttgttccata
     3241 cggtgattta gaagcattaa aagctgcgat tacaccaaac acagcagcgt ttttggttga
     3301 gccaatccaa ggtgaagctg gcattcttat tccacctgaa ggtttcttgg aacaggctta
     3361 tgaactatgc aaagagaata atgtgctgtt tattgctgat gaaattcagg caggtcttgg
     3421 gcgtactggc aagttatttg caacagaatg ggaaggcttt gagccagata tttatattct
     3481 aggtaaagca cttggtggcg gggtgttccc aatctcttgt gtgttagcaa atcgtgatat
     3541 tttaggcgta ttcaatcctg gctcgcacgg ttcgacattt ggtggtaatc cattagcctg
     3601 tgcggtatct attgcctcaa ttgatgtact gttggaagaa aaacttgcag agcgttcccg
     3661 tgagttaggt gaatacttct tggcagagct gaaaaaaatc caaaatccaa ttattaaaga
     3721 agttcgtgga cgtggactgt ttattggctt ggagttacat caaccagcac gcccattctg
     3781 cgaaaaactc aaagaaaaag gcttactctg caaagagacc cacgatacag ttatccgctt
     3841 tgctccccct ttggtgattg aaaaattcga tttggattgg gcattggaga aaattcggga
     3901 ggtgttggct taattgtcat tttctctata ttttatccta ccaaatcgcc tttaaccctt
     3961 tttggcagga agcaaaacag ttgtaaaatg ctgattttgc aaactttttc caaaaatcaa
     4021 ccgcttgtaa aaggtggctt tatgtcaaac agaacagaaa taacagacca aaatatccga
     4081 atcccgtttc gctatgctat attgcctgtg attattatgg tatcgattat ggcggtggct
     4141 atcatcaaat ttgaagcctc acctcacgtt ccgttgctta ttggggcaat tagtgccgct
     4201 gtgattgcga tggcacatgg ctaccgttgg gagacaattg aaaaaggaat ttatagaggc
     4261 attcgtatgg ctttaccagc gattgttatc attattatga ttgggctaac catcggagct
     4321 tggctaggtg gtggaattgt agctacgatg atttactacg gcttgaaatt gatctctccg
     4381 tcgctgtttt tagtaactat ctcaataatt tgtgctattg tgacccttgc gattggtagc
     4441 tcgtggtcta caatgggaac aatcggtata gcggcgatgg ggatcgggtt tagtctcggc
     4501 attccggcac cgatggttgc cggtgcggtg atctcaggag catatttcgg cgataaaatg
     4561 tcgccactgt cagacactac caatctggca gcagggatta ccgaaaccaa tctgtttgaa
     4621 catatccgcc atatgtttat cactaccgtg ccggcgttcc tgattgcgtt atctgtctat
     4681 ttctattttg gtatggaatt tagagccgaa aatctcgatc ttgctaaaat tgaagacatt
     4741 atgcaggcaa tgcaggaaca tttcttgatt tcgccttggt tgctattagt gcctttatta
     4801 gtcgtggtgt tggtggcaaa aaaagtgcct gccttaccgg cattagctgc agggataatt
     4861 cttggttggc tctgccacgt ttttgtgcaa ggcggtgatg tcagcgtagc agtgattact
     4921 ctgcacgacg gctataaaat tgagagcggc cacgagatga ttgataagct gttcaaccgt
     4981 ggtgggatcg agtcgatgat gtacaccatt tcccttacga ttgttgcgat gacttttggt
     5041 ggcattgcag agcaagctgg aatgttacat tcagtagtgg aaaaaatcct tacccttgca
     5101 aaatcagcag gtagcttaat tagtgctacg attgtatcgg catttattac caacgtaacg
     5161 acatctgagc aatatatttc tattttatta ccaggcagaa tgtatataaa agcctatagg
     5221 gataagggac taagttcgag aaacctttct cgtgcattgg aagacggagg aacagttact
     5281 tcggttcttg taccttggaa tacctgtggt gtttatgcgt tctcaatgtt gcaagtgagt
     5341 gcttttgaat atgcaccgta tgcaataatg aattggctca ttccactgat tgcgattggc
     5401 ttggcaacct tgaatatcaa aattgagcga ttgccaccgg aagaattagc caaagaacct
     5461 gaggaaatta ctctgccaaa acgtggttgt tgtccataat ttatctaaat agtatttaat
     5521 aacaataggt taagttaaat aacaagcggt cggttttatc ttaaaatttg caagtttata
     5581 cagaaatttg atcgcttgct gtatagaaag gaaaaatgat gtcataccaa cttttaccta
     5641 acaccactat ccgtcctgaa ttgagccgtc attaccgctt tgatgagcaa accctggtgc
     5701 aaaatctgat agaacaagca cattgtgatg aacaggctga taacattaaa aagctcacta
     5761 cccaattagt cggtaaagtc cgaactgcac gccagaaagc aagcggggtt gatgcgttaa
     5821 tgcacgaatt ttcactctcc agccaagagg gggtggcgtt gatgtgcctt gccgaagcat
     5881 tactacgcat tcctgacacc gccactgcgg ataaattaat tcgagacaaa attgccaaag
     5941 gggattggcg ttcacacacc ggcaacagcc cctctctgtt tgtgaatgca gcggcgtggg
     6001 gcttggtgat taccggcaaa ttggtttcaa cccacagcga aagctcactc tcatcagcac
     6061 tttcacgcct gatcgccaaa ggtggagaac cgctgatccg caaaggtgtg gacgtagcaa
     6121 tgcgtatgct cggcaagcaa tttgtaacgg gcgaaaccat tcaacaggcg attaaaaacg
     6181 gcgaaaaacg cttcgcaatg ggttatcgtt acagctacga tatgctgggc gaagcggcac
     6241 tcaccgccga agatgccaaa cgctattaca acgactatgt tgcctctatt cacgctgtgg
     6301 gtgccacctc gaaaggcttg ggcgtgtata aatcctcggg tgtgtcggtc aaactctccg
     6361 ccattcaccc tcgttattcc ctttcccaac accaacgtgt gatggacgaa ctctatccac
     6421 gtctgaaaga actgttcttg ctggcgaaaa agtatgacat cggcttgaac attgatgccg
     6481 aagaagccga ccgcctcgaa ttgtcgcttg atttgatgga cagactgctt gccgaccctg
     6541 atttggcagg gtttaacggc attggttttg tggtgcaggc gtaccaaaaa cgttgcccgt
     6601 atgtgatcga gcatttaatt gagaccgccc gtaaacataa tcgccagcta atgattcgct
     6661 tggtaaaagg cgcatattgg gacactgaaa tcaagcgggc gcaaatggac ggtgcagagg
     6721 gctatccggt cttctcccgt aaggtgcata ccgatttgaa ctacatcgtt tgtgccaaaa
     6781 aattgctggc ggcgcaagat gtgatctatc cgcaatttgc cacccacaac gctcaaactc
     6841 tcgccaccat ttaccacttg gcacagggca aacgttttga gtttcaatgc ttgcacggta
     6901 tgggagagag cttgtacgac caagtggttg gcagcgataa tcttaacgtg caatgtcgca
     6961 tttatgcccc tgtcggcact tatcaaaccc tgcttgccta tttagtacgt cgcttgttgg
     7021 aaaacggagc gaacagctca tttgttagcc aaattgtgga tgaaaatctc agtgtagatg
     7081 atctggctca agaccctgtt caaaaagcag aactgactaa tggcactatg cacccgaaaa
     7141 ttccattgcc agtcgattta tttgacgaca atcgtgttaa ttccaaaggc tatgatttaa
     7201 ctgatgcagt aagcctacaa aatttagaaa atgccctcaa tgaacttacc aaacagcatt
     7261 accgtgctga accacagctg gcaaatgata atacaagcgg tcaagaaagc cgcaaaattt
     7321 acaatccagc gaaccaagct gaattagtcg gcgaagtgat tgatgccacc gaaaacgata
     7381 tggaacaggc gtttgaaagt gcggcggcgt tccaaggtga atgggcgaat acttcccctg
     7441 cggtgcgtgc caagtgctta gaaaaaatgg cggacttgat ggaagccaat atgccacaac
     7501 tgttcgattt ggcggtgcgt gaggcaggca aaacgctcaa caatgcaatt gctgaagtgc
     7561 gtgaagccgt cgatttctgt cgttattatg ccaaagaggt acgcaacaac attgaagcat
     7621 acaaaaatcc gcgtggcatt gtagtagcaa ttagcccgtg gaactttccg ttagcgattt
     7681 ttgtgggtga agtttcatct gcgttggtgg caggcaatgt ggtactagca aaaccagctg
     7741 aacaaacttc gttaatggca cattatgctg ccagcctttt ctaccaagca ggtgtgccga
     7801 aagcggcgtt gcaatgctta ccgggtagag gaaaaacggt tggggtggcg ttggtggcag
     7861 atcatcgtgt gaacggcgtg atttttactg gttcaacaga gacagctaaa aacattaacc
     7921 gcaatttgca caaaaacgag aaaattatac cgcttattgc cgaaactggc ggacaaaatg
     7981 caatgattgt tgatagctcg gcactgggcg agcaggttgt gatagatgtt ttaaactccg
     8041 catttgactc agcagggcaa cgttgttcgg cattgcgtgt gctgtatctg caacgtgatg
     8101 ttgccgatca cattctcaca atgctcaaag gggcaatggc ggagttgaaa gttggtcttc
     8161 cacaacactt aactaccgat gtcggaccag taatcgataa agtcgcacaa caacgcttgc
     8221 tcgatcatat tgaaaacctc aaaaaatcgg cgaaaagtta ctatcaggtg gctatctcct
     8281 ctgaaattgc cgaaaatggt attttcgtac cgccaacgct gttagaaata gatcatatca
     8341 gccagcttaa acacgaagta ttcggaccgg tgctacacgt tatccgtttt gatgcggata
     8401 aactcgacca agtgattgcc gacataaact ccacaggttt cggtctaacc agcggtttgc
     8461 atagccgaat tgatgaaacg gtggatttat ggttgaaatc tgtccacgct ggaaatctgt
     8521 atgtaaaccg taacacggta ggggctgtgg tcggcgtaca gccattcggt ggaatggggt
     8581 tatcaggaac tgggccgaaa gctggcgggc cgctctatct acaacgttta gtaaaccgcc
     8641 aagaatggac attgaacggt ttacagggca atgcacaagc ggtcgatttt gacttaaatt
     8701 ttgcaatccg caaggttcta agtggagctg agcagcaaac ggcgttagat ctggccaaaa
     8761 gccttgcacg ccgctcgcca ctcggaaaaa cctacccaat gcagggtatt accggcgaga
     8821 aaaacgagat gattttcgtg gctcgcccac agattgggat tggcaaaggt gatttggctt
     8881 cacaggtgca agccttgatt gccattgtaa cggtcggcag ccaagcggtg gtagaaaaat
     8941 caagcgagct ggcaaaacac gcctctgcct ttggtaagaa attacaggtg gtggatgatt
     9001 tcaacagtct cgatcacctc agcacactca ttgtgcttga cctgctttct agtgaggaaa
     9061 aattgcatca tgccgaacgc aaaggggcga ttttgaccta catcgaaggg ctggatttag
     9121 cgctctcact gctgccgttg gtacacgaga aagcaatcag catcaacacc gctgccgcag
     9181 ggggaaatgc cagcttaatg agtgaaatgg attaagcaac aaacaaaaga gtataagagg
     9241 gcagatcaca atgatctgcc tttttatttt aaggggctgt cctagataac gactaaaact
     9301 tccattaggt t
//
LOCUS       DANJWC010000092         9234 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.092, whole genome shotgun sequence.
ACCESSION   DANJWC010000092 DANJWC010000000
VERSION     DANJWC010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9234
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.092"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            36..1238
                     /locus_tag="PWX02_001564"
                     /pseudo
     CDS             36..1238
                     /locus_tag="PWX02_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252774.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
     gene            1264..4422
                     /locus_tag="PWX02_001565"
     CDS             1264..4422
                     /locus_tag="PWX02_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDL1729561.1"
                     /translation="MLGINENTIEQSAIATLQGLGWDYTYGKTILAGLEHEWRERATE
                     VVLKPLLAEAIAKFNPNLPACEVENVVAQVCRAESSDLAERNRQAYDWLRNGVKITYQ
                     LDGEQVSDVVQLIDFQRPENNDFRIVNQLDIGGKKGKRIPDLIGFVNGLSLVVFELKN
                     PLKENADIGKAFAQLQTYKDEISDLFVFNQALVISDSIVARIGSLTADFDRFTPWRVV
                     DEKNQNKRIVFEDELTALLQGVMNPKNLLDYVQNFVVFERDGKNRLIKKIGAYHQFYG
                     VNEAVDCTLLATTGNRKIGVFWHTQGSGKSLSMLFYAGKVLSQGCLKNPTLVLVTERN
                     DLDGQLYATFCGGEALLKQTPIQADGRDELRSALASRSAGGVIFTTIQKFGLMEGELA
                     HPVLNERENIIVITDEAHRSQYGFSQKINHKGEYREGYAKHLRSALPNASFIGFTGTP
                     IELDDKDTQEVFGKYVSIYDFEDAVEDGATVPIIYEPRQISLGESGEFTKVMEEAQQL
                     IDDDENSYNFRLREKLHSVDSRLQKMAEDIIAHYDERTKQQDGKAMVVVMSRAICVKL
                     YEKITALRPEWHSNDVLQGSIKIVMTSNASDPAEWQKHNQDKKTLEKRFKDPDDPLNI
                     VIVRDMWLTGFDAPCCNTMYIDKPMSGHNLMQAIARVNRVFRNKSRENGGLIVDYVGL
                     TDELEKAMKQYTNAGGKEKPVRDISAVLEKMVEHITVIRGQFATPIDGQAVDIAKMLQ
                     ISEPPKLLNAILTSANHILALDRIQPPDNTAKDKTPRKNAFLQSVRLAKKGYALCGAL
                     KAVEPYKQELAFYDAVRATIIKNSTAPRNSSSENDRLLQLTALMNRAVQSDGVVDLFD
                     LLKKDRPNINLLSDEFLETVKNSPTKDLWLSAMERYLASQLREQSGANLATKKAFEQK
                     LKEAMNQYHNHNLSVLEILEELIALAKEFEARQKRGEALGLSPAEMAFYDALARNESA
                     VREMGDEVLMKLAKDITDKLRKSVTVDWQYKDSVRAKMRTLIRIALRTYKYPPDLQAE
                     AIEFVLQQAEEIAGEFAITETA"
     gene            complement(4456..7347)
                     /gene="glnE"
                     /locus_tag="PWX02_001566"
     CDS             complement(4456..7347)
                     /gene="glnE"
                     /locus_tag="PWX02_001566"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HDL1729562.1"
                     /translation="MLTTLIPLSQLNLPPNLQNSEQIQPLVTAISLSNFVHQTLQKQP
                     DLLAEWLAKFPTPDDCCNYTERLAKILARVENEEMLGRVLRQFRHRELARLSFIQSNK
                     LATVELVFQHLSDLAESLILAARDWLFQRCCAEYGTPKNTLGETQELLILGMGKLGGR
                     ELNFSSDIDLIFTYPDIGETEGGRKPIENSKFFTRMGQRLIKVLDEITADGFVYRTDM
                     RLRPFGDSGALVLSFTAMEDYYQEQGRDWERYAMIKAKILGEDSQNLNHRYLKQMLRP
                     FVYRRYLDFSAIQSLREMKEKISREVVRRNLTDNIKFGAGGIREVEFIAQTFQMIRGG
                     RDKILQERSLLKVLPRLAELNLLTQTQVETLHNAYIFYRQIENVLQAIDDKQTQTLPT
                     DEASQARLVFACQSYYQQDLYSEKTHWVEHSFNDWQQFMAVLSQYQQAVRQIFNEIIG
                     EEETQANCNPVNEKLAEWKDILHYNIRLEDLSAVLKGYPKVAENDYTEIFRHFSTTFQ
                     DWVKRPIGVRGREVLRNLMPRIADSIFKGEDHLLLLPRVLNIVDKITTRTTYLELMLE
                     KEQILPQLLALCSKSVMIAEQIARYPMLLDELMSHRGLTDVQAFEKYQSALQDYLIRI
                     PEEDEEALIDGLRQFKQTQILRIAAADILGVLLVMKISDHLTYLAEAIINAVVNMAWK
                     QVSQRFGVPEHLEADEKGFAVIGYGKLGGIELGYNSDLDLVFLHNAPEDSQTVGGKKE
                     ISSHQFYLKLAQKINSIFNLNTSAGVLYEVDMRLRPSGEAGLLVSTFNAYEHYQKNEA
                     WTWESQALVRTRCVFGAENLKQAFEKIRQSTLAQPRASGQLRQEICEMRQKMYQHLSS
                     HSAEQFHIKQDQGGITDIEFIAQYLVLAHSHQHPKMAVWSDNVRIFDSAVECGILSSE
                     QSEQLQHCYTALRNKIHHLNLLRKDSVVDASEFTTERAFVREMWQRLLA"
     gene            complement(7519..7800)
                     /locus_tag="PWX02_001567"
     CDS             complement(7519..7800)
                     /locus_tag="PWX02_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HDL1729563.1"
                     /translation="MYYVIFAQDNPNMLEKRLEVRPAHLARLEKLQAEGRLLIAGPNP
                     TEDGSSVTGSTVIAEFNSLEEAQQWASQDPYVEAGVYGDVIIKPFKRVF"
     gene            complement(7802..8266)
                     /gene="yciA"
                     /locus_tag="PWX02_001568"
     CDS             complement(7802..8266)
                     /gene="yciA"
                     /locus_tag="PWX02_001568"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HDL1729564.1"
                     /translation="MKDKPYGKAPEGKLILRTLAMPSDTNANGDIFGGWIMSQMDLGG
                     AILAKELAKGRVVTVTVDKMIFHRPISVGDVVCCYGKLMHIGRSSMQVKVEVFIKQVY
                     EGNRERYLVTEALFTYVAIDKEGKPRAVPRENNPELDEALSLIDLTPEKQGE"
     gene            complement(8256..8813)
                     /locus_tag="PWX02_001569"
     CDS             complement(8256..8813)
                     /locus_tag="PWX02_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HDL1729565.1"
                     /translation="MKQLLEFIPLILFFVVYKMSGIQMASIALVIATIVQMIALKMLY
                     GKIEKQQIIMGGSVVFFGLLSAYFNEVKYLQWKVTIVYALFALVLLVSQFVFKTPLIK
                     KLLGKEIQLPEAVWNKINFGWAVFFLICMLLNIYISQYLSEEIWVDFKSFGIIGLTLV
                     ATVITGFVLYPYLKDAEKQEQKNER"
     gene            complement(8822..>9234)
                     /locus_tag="PWX02_001570"
     CDS             complement(8822..>9234)
                     /locus_tag="PWX02_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1729566.1"
                     /translation="LLLQFVMVLPLSLGASFAMASPETVIIALPVLVVGFYFFIKLSL
                     VIYAYLLEKPQKSLSESIKFTLQLSRGKMLPLILFCVISYLLPGMLSRLFTVFGNDFI
                     GIFITIVLSAVINVFMAIFSFRFYQVYRQMPAVR"
BASE COUNT         2778 a         1954 c         1930 g         2572 t
ORIGIN      
        1 gttactacct aaattattaa atggagagtt attcaatgaa taacgaagaa ttatcttcta
       61 gttactttga tgaaatcaaa gaatggcaaa gtggcgaacc tatttttgtc tctaaaaacg
      121 atgagataaa tagtcgtgtc cctttattaa gagtaaatgg ctggaaaaag tttattgatt
      181 tactttctac aaattttttc caagaaaata tttctgaact catttttaga gggcaacgcc
      241 gatatgattg gggactaatg cccaatattg ccagaaatag tgataatggc ttgtttacta
      301 aagaattgat ggaaaatcag cttaataact ttaaaaaagc cattcgaggt cggattaaag
      361 attatacttt attgcttaat gaagatgatg atgaaatttg ggcaattggg caacattatg
      421 gattgaatac acctttattg gattggacat attctcctta tgtctcatta ttttttgctt
      481 tttcacgcaa agatgttaaa gctgaaaagc caaatgaata ccgagttgtt tatattgcaa
      541 ataagaagtt actttctaac tttgttacct ttgttgatcc caaagtggat atgtatggga
      601 gattggtcaa tcaagcaggt ttatttatta aagcagaagc aggaaaatcg atagaatctt
      661 taattgtaga ttctattgcg gagcagaacg aaagtgaatt tctctctgat gagtcattcg
      721 aacaagaaat agcaaaatat ctctgaaaaa tttatatttt aaacacacca gacattcaaa
      781 gtgattgcat tttgtctttg cgacaaatga atattcatca cgccaattta tttcctgatt
      841 taatgggggc tgctgactat tgcaatctac tttctgaatt tcagccatat aacaaaaacc
      901 aaaaaattat tgatagttca acgccaaatc attgggagtc tccaactctt gcaaataacg
      961 aaggtagtat ccctactctt acaaacaata gctctattaa taatattaag gaattattaa
     1021 taccttatct agctcttcct gatgcagacc tagaacaaat agccaataag ttactcaata
     1081 ttctaaaaac caatatggta actgactggc aaacacgaga tgaaattaaa gcaaaaatca
     1141 aaattgcatg gcgtagaatc ctatctcaaa ccaacatgaa taaagaacaa atagaaaata
     1201 tgttagataa attattcaca ttagattttc agccataaac attattttca cggagaaccc
     1261 acaatgctag gcatcaacga aaacaccatt gaacaatccg ccattgcgac tttgcaaggc
     1321 ttgggctggg actacaccta cggcaaaacg attttggcag gtttagaaca cgaatggcgg
     1381 gagcgagcca ctgaggtggt attaaagccg ctgcttgccg aagcgattgc aaaattcaac
     1441 ccgaatttgc ccgcttgtga ggtggaaaat gtggtggcac aggtgtgccg tgcggaaagt
     1501 agtgatttgg cggagcgtaa tcgtcaggct tatgattggc taagaaatgg ggtcaaaatc
     1561 acttatcagt tggacggtga gcaggtgtct gatgtggtgc agctgattga ttttcagcgt
     1621 ccagaaaata acgattttcg cattgtcaat cagctggata ttggcggtaa aaaaggcaaa
     1681 cgcattcctg atttgattgg ctttgtcaat ggcttgtcgc tggtggtatt tgagctgaaa
     1741 aatccgctca aagaaaatgc cgacatcggc aaggcgtttg cccaactgca aacctataaa
     1801 gatgaaattt ctgatttgtt tgtgtttaat caggcactcg tgatttcaga cagcattgtc
     1861 gcccgtatcg gttcgctgac cgccgatttc gaccgtttta ccccttggcg tgtggttgat
     1921 gaaaaaaatc agaacaaacg cattgtgttt gaagatgaac tcaccgccct actgcaaggc
     1981 gtgatgaacc ccaaaaatct actggattat gtgcagaatt ttgtggtgtt tgaacgggac
     2041 ggcaaaaacc gtttgattaa gaaaatcggg gcgtatcatc agttttatgg cgtgaatgaa
     2101 gcggtggatt gcaccttgct tgccaccaca ggcaaccgca aaatcggtgt gttttggcat
     2161 acgcaaggct cgggcaaatc gctttcgatg ctgttttatg caggtaaagt attaagccaa
     2221 ggctgcctga aaaatccaac tttggtgtta gtcaccgaac gcaacgattt agacggtcag
     2281 ctttacgcca ctttttgcgg tggtgaggca ttgctcaaac aaacgcctat ccaagccgat
     2341 gggcgagacg aactccgctc tgcccttgcc agtcgttcag caggcggtgt aatttttacc
     2401 accattcaaa aatttggctt aatggagggc gagcttgccc acccagtgtt aaacgagcgg
     2461 gaaaacatca ttgtgattac cgatgaagca caccgttcgc aatatggttt tagccaaaaa
     2521 atcaaccaca aaggcgagta tcgtgagggt tacgccaagc atttacgcag tgcattgccg
     2581 aatgcttctt ttattggttt tacaggcacg ccgattgaac ttgatgacaa agacacccaa
     2641 gaagtcttcg gtaaatatgt ctcgatttat gattttgaag atgcggtgga agatggggca
     2701 accgtgccga ttatttatga gccacgccaa atcagcttag gcgaaagtgg cgagtttacc
     2761 aaagtgatgg aagaggcaca acaactgatt gatgacgatg aaaacagcta taacttccgc
     2821 ctgcgtgaaa aactgcacag cgtggatagc cgtttgcaaa aaatggcgga agatattatt
     2881 gcccattacg atgagcgtac caaacagcaa gacggcaagg cgatggttgt ggtgatgagc
     2941 cgtgccattt gcgtgaaatt gtacgaaaaa atcaccgcac ttcgtccaga atggcactca
     3001 aacgatgtgc ttcaaggcag cattaaaatt gtgatgacaa gtaatgccag cgaccctgcc
     3061 gagtggcaaa aacacaatca ggataagaaa accttagaaa aacgctttaa agacccagac
     3121 gatccgctca acatcgtgat tgtgcgggat atgtggctga caggctttga tgcgccctgc
     3181 tgtaacacaa tgtacattga taaaccaatg agcggacaca acctgatgca ggcaatcgct
     3241 cgggtaaatc gggtatttcg caacaaaagc cgtgaaaatg gcggcttgat tgtggattat
     3301 gtgggcttga ccgatgagct ggaaaaagcg atgaagcagt acaccaacgc aggcggcaaa
     3361 gaaaagccgg tgcgggacat ttcagcggtg ctggaaaaaa tggtggaaca tatcacggtc
     3421 attcgtgggc aatttgccac accgattgac ggacaagcgg ttgatattgc caaaatgttg
     3481 caaatcagcg aaccgcctaa actgctcaat gcgattttga catcagccaa ccatattctt
     3541 gccctagacc gcattcagcc acctgataac actgccaaag acaaaacccc acgcaaaaac
     3601 gcctttttgc aatcggtacg cttggcgaaa aaaggttatg ctttgtgtgg ggcattaaaa
     3661 gcagttgaac cctataaaca ggaactggcg ttttatgatg cagtgcgtgc caccattatc
     3721 aaaaacagca ccgctcctcg caattcttcg agcgaaaatg accgcttgtt gcagcttacc
     3781 gccttgatga atcgtgcggt acagtcggat ggtgtggtgg atttatttga tttgctgaaa
     3841 aaagaccgcc caaacatcaa cctgctttct gatgagtttt tggaaacagt aaaaaacagc
     3901 ccaaccaaag atttgtggct atcagcaatg gagcgttatc ttgcctcaca actgcgagag
     3961 caaagcggtg ccaatcttgc cacaaaaaaa gcatttgaac agaaactcaa agaggcgatg
     4021 aaccaatacc acaaccacaa tttaagcgta ttggaaattt tagaagagct gatcgccctc
     4081 gccaaagagt ttgaagcccg ccaaaaacga ggggaagcgt tgggactaag ccctgcggaa
     4141 atggcgtttt atgatgcttt ggcacgtaat gaaagtgcgg tgcgggaaat gggcgatgag
     4201 gtattgatga aacttgccaa agacattacc gataaattac gcaaatcggt caccgtagat
     4261 tggcaatata aagactcggt tcgtgccaaa atgcgaacct taatccgcat tgctctgcgt
     4321 acctataaat acccgcccga tttacaggcg gaagcgattg agtttgtgtt gcaacaagcg
     4381 gaagagatcg ccggagagtt cgctatcacc gaaacggcgt aatctatatt agggcgtgca
     4441 atgtacgccc taaatttatg ctaataacct ctgccacatt tcccgcacaa atgctcgctc
     4501 ggtggtaaat tcacttgcgt ccaccacgct gtctttgcgt aataagttta ggtggtggat
     4561 tttgttacgc agtgcggtgt agcaatgttg tagctgttcg ctttgttcgg aacttaaaat
     4621 accgcattct actgcgctgt cgaaaattcg gacattatcc gaccatactg ccattttcgg
     4681 gtgttggtgg ctgtgggcta ataccaaata ttgggcgata aattcaatat cggtaatacc
     4741 tccttgatct tgtttaatat gaaattgctc ggctgaatgc gagcttaaat gttggtacat
     4801 tttctgtcgc atttcgcaaa tttcttggcg taattgaccg cttgcacgag gctgagcaag
     4861 cgtgctttgg cggatttttt caaaggcttg tttgaggttt tccgccccaa atacacatct
     4921 tgtccgcacg agggcttggc tttcccacgt ccacgcttca tttttttgat aatgctcgta
     4981 ggcattgaaa gtgctgacta aaagcccagc ctcgcctgat gggcgaagtc gcatatccac
     5041 ttcatatagt acgccggcac tggtattaag gttaaaaatc gagttaattt tttgagccaa
     5101 ttttaagtaa aattgatggc ttgaaatctc ttttttaccg ccaacagttt ggctatcctc
     5161 tggggcatta tgcaagaaaa ccaagtcgag atctgaatta tagcccagtt caatcccgcc
     5221 tagcttgccg tagccaataa ctgcaaaccc tttttcatca gcctctaaat gttccggcac
     5281 accaaagcgt tggctgactt gcttccacgc catatttacc accgcattaa taatcgcttc
     5341 tgctaagtaa gtaagatgat cgctgatctt cattaccagc agcacgccta aaatatcagc
     5401 agcggcgatg cgtaaaattt gggtttgttt gaactgacgt aagccatcaa tcagtgcttc
     5461 ttcatcttct tcaggaatgc ggattaaata atcctgcaag gcagattgat atttttcaaa
     5521 agcctgcacg tcggttaagc ctctatggct catcagctca tcaagtagca ttggatatcg
     5581 agctatctgt tccgcaatca taaccgactt actacacaat gctaaaagtt gaggcagaat
     5641 ctgctctttt tcgagcatta attccaaata ggtagttcgg gtggtgattt tatcgacaat
     5701 attcaacacg cgaggcaata acaataaatg atcttcacct ttaaaaatgc tgtcggcaat
     5761 tctaggcatt aaattgcgta gcacctctcg ccctcttacc ccaatcgggc gttttaccca
     5821 atcttgaaag gtagtagaaa agtgacggaa aatctccgta taatcatttt ctgcaacctt
     5881 tggataacct tttagcactg cggataaatc ttcaaggcga atgttatagt gcaaaatgtc
     5941 tttccattcg gctaattttt cattaaccgg attgcagttg gcttgtgttt cctcttcacc
     6001 gataatttca ttaaaaatct ggcgtactgc ttgttgatat tggctcaata ctgccataaa
     6061 ttgttgccaa tcgttaaagg aatgctccac ccaatgcgtt ttttcgctat atagatcttg
     6121 ctgataatag ctttggcaag caaatacaag gcgggcttgc gaggcttcat cagtgggtag
     6181 ggtttgagtt tgtttatcgt caatcgcttg taatacattt tcaatttgac ggtaaaaaat
     6241 ataggcattg tggagcgttt ccacctgcgt ttgggttaat aaatttaatt ctgccaagcg
     6301 tggtagcact tttaacaggc tacgttcttg taaaatttta tctcgcccgc cacggatcat
     6361 ttggaaagtt tgggcgataa actccacttc ccgaatccca ccggcaccaa atttaatatt
     6421 atcggttaaa ttacggcgaa ccacttcacg gctgattttt tctttcattt cacgcaacga
     6481 ttggattgca ctgaaatcca gataacggcg gtaaacaaaa gggcggagca tttgctttaa
     6541 atagcgatga tttaaatttt ggctatcctc gcctaaaatt tttgctttaa tcatcgcata
     6601 acgttcccaa tcacgccctt gctcttggta ataatcttcc attgcagtaa aactcagcac
     6661 caaggctccg ctatcaccaa aggggcgaag tcgcatatcg gtacgataca cgaaaccatc
     6721 agcggtgatc tcatctaata ctttaattaa ccgctgcccc attcgggtaa aaaatttgct
     6781 gttttcaatc ggtttgcgtc caccttcggt ttcaccaata tcggggtagg taaaaatcag
     6841 gtcaatatcg gaagaaaaat taagctctcg tccgcccaat ttgcccatac ctaaaatcag
     6901 cagttcttgt gtttcgccaa gagtgttctt tggcgtgcca tattcggcac agcaacgctg
     6961 aaacaaccaa tctcgagcgg ctagaatcag gctctcggct aaatccgaca aatgctgaaa
     7021 taccaattct accgtcgcta atttatttga ctgaataaag ctcagacgag ccaattcacg
     7081 gtggcgaaat tgccgcaaaa ccctccccaa catttcttca ttttccactc ttgctaatat
     7141 ttttgctaaa cgctcagtat aattgcagca atcatcaggc gtaggaaact tggcaagcca
     7201 ttctgccagt aaatccggct gtttttgcaa ggtttgatgc acgaaattgg aaagcgaaat
     7261 cgccgtcaca agcggttgaa tttgctcaga attttgcaaa ttgggcggta aattgagttg
     7321 ggataaggga attagggtgg taagcataaa tttaggctca aaattgaaaa tagatagaat
     7381 tttacgctcg atcaattccc ctctttagca aagagggctt aggggagatt tggcagaggt
     7441 aattttactc tcatacgaaa cattaatatc gtaatcaaat ctcccccagc ccctcttttc
     7501 taaagagggg agaaaaaatt aaaacactct tttaaacggt ttaataatca catcgccata
     7561 cacaccggct tccacataag gatcttggct tgcccattgt tgagcttctt caagtgagtt
     7621 gaactctgca attacagttg aaccggttac gcttgagccg tcttcagtcg ggttaggccc
     7681 tgcgattaat aatcgacctt ctgcctgtaa tttttccaaa cgagccaaat gagccgggcg
     7741 aacttctaaa cgtttttcca acatattggg attatcttgg gcaaagataa cataatacat
     7801 actattctcc ttgtttttca ggggttaaat caattagcga taatgcttcg tctaattcgg
     7861 ggttattttc tctcggcaca gcacgcggtt tgccttcttt atcaatcgct acataggtaa
     7921 ataaggcttc ggttacgaga taacgttcgc gattgccctc ataaacttgt ttgataaaga
     7981 cctcgacttt cacttgcatc gaagaacgcc ctatgtgcat taacttgccg tagcagcaga
     8041 ccacatcacc gactgaaatc gggcggtgga aaatcatttt atctaccgtt accgtaacga
     8101 ctctaccttt tgccaactct ttggctaaaa ttgcaccgcc taaatccatc tgcgacataa
     8161 tccaaccgcc gaaaatatcg ccattggcgt tagtatctga cggcattgcc aaagttctta
     8221 aaatcagttt cccttccggg gcttttccat agggtttatc tttcattttt ttgttcctgc
     8281 ttttcagcat cttttaagta agggtaaagg acgaaaccgg taataaccgt tgcgactaag
     8341 gttaagccaa taatgccaaa ggatttgaaa tcaacccaaa tctcttccga taaatattga
     8401 ctgatataaa tattcagcag catacaaatc agaaagaata ctgcccaacc gaagtttatt
     8461 ttattccaca ctgcttcagg caactgaatt tctttgccta ataatttttt gattaatggt
     8521 gttttgaaca caaattggct cactaatagc accaatgcaa acagagcgta aacaatggtt
     8581 actttccatt gtaaatattt aacttcatta aaataggcac tcagtaagcc gaaaaagacc
     8641 accgagccac ccataataat ttgctgtttt tcaattttgc cgtagagcat ttttaaggcg
     8701 atcatttgca cgattgtagc gataaccaat gcgatagacg ccatctgaat accgctcatt
     8761 ttatacacga caaaaaacag aatcagcgga ataaattcaa gtaactgttt cattgatact
     8821 cctaacgcac tgccggcatt tggcggtaaa cttggtaaaa gcggaagctg aaaatcgcca
     8881 taaagacgtt aattaccgca cttaaaacaa tggtaatgaa tatgccaata aagtcattac
     8941 caaacactgt gaacaaacga gataacatcc ccggtaataa ataagaaatc acacaaaata
     9001 aaatcagcgg tagcattttt ccacgtgata attgaagcgt aaatttaata ctttccgata
     9061 gacttttctg cggtttctcc aataaataag cataaatcac taacgataat ttgataaaaa
     9121 aataaaaacc gactaccaac accggcaatg caataatcac tgtttcaggc gatgccattg
     9181 caaaagatgc accgagagaa agcggtaaca ccatcacaaa ttgtaacaac agca
//
LOCUS       DANJWC010000093         9165 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.093, whole genome shotgun sequence.
ACCESSION   DANJWC010000093 DANJWC010000000
VERSION     DANJWC010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9165
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.093"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            96..1202
                     /gene="mltA"
                     /locus_tag="PWX02_001571"
     CDS             96..1202
                     /gene="mltA"
                     /locus_tag="PWX02_001571"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HDL1729567.1"
                     /translation="MNWKAYKKAIAAASVVAIATVLVACSSSGQKGKTTSYDAEKAKF
                     GAKYNGRHFMPYGTHFQAGRLGLNAAPVNQLDFLQQLSNVRAYSSLADSYAIAYGKIS
                     RWISSGGNINDLAKYGISTRLMRGEDGYQNVLMTGYYSPVIKARRTPQGQYQHPIYAM
                     PSSKRFSRAEIYAGALQGQGLELAYSNSMLDNFLLGVQGSGYVDFGDGRLNYFAYAGQ
                     NGFKYASVGRLLVEDGEIPKEKMSVQAIREWGQRNPSRVQGLLERNPSYVFFKNDPTG
                     QVKGSAGVPLVPLASVASDRSIVPSGSVLLVEMPLIDSKGNWTGKHEMRLMVALDVGG
                     AVKGHHFDLYQGIGDKAGHIAGLMKHYGRVWVLN"
     gene            1273..1677
                     /locus_tag="PWX02_001572"
     CDS             1273..1677
                     /locus_tag="PWX02_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729568.1"
                     /translation="MLANNLQNITASTEPKYKISEIDVRILIRQLNNIEQCIYPELAK
                     PGYQQIYANWNLAENLTMQYFEYQLLKELLGEENQKLMQNDTLSTEYFHLLHSRLNHQ
                     KANVDPEKCDTFKPRYKEIYKSMENALTKKNQ"
     gene            complement(1711..3228)
                     /gene="purF"
                     /locus_tag="PWX02_001573"
     CDS             complement(1711..3228)
                     /gene="purF"
                     /locus_tag="PWX02_001573"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL1729569.1"
                     /translation="MCGIVGIIGNSPVNQAIYDGLTLLQHRGQDAAGIVTIDDENRFR
                     LRKANGLVSDVFQQEHMVRLQGNVGIGHVRYPTAGSSSVSEAQPFYVNSPFGITLVHN
                     GNLTNNAELKARLYNEARRHVNTNSDSESLLNIFAYFLDLYSTQHLSPDNIFETVRKT
                     NDSIRGAYACIAMIIGHGMVAFRDPFGIRPLVLGKREIEGKTEYMFASESVALDVVGF
                     EFVRDVLPGEAIYVTFDGQLHSQICADNPKLNPCIFEYVYFARPDSVIDGVSVYSARV
                     HMGELLGEKIKREWGRIIDDIDVVIPIPETSNDIAVRIANMLYKPYRQGFVKNRYVAR
                     TFIMPGQAQRKSSVRRKLNAIASEFKGKSVLLVDDSIVRGTTSEQIVEMARAAGAKRV
                     YFASAAPEIRYPNVYGIDMPTCEELVAYDRSVEEVAQMIGVDKLIFQDLEALYKSIQL
                     ENPTIHRFDDSVFTGEYITGDVDKCYLDSIARSRNDKAKAEAAKQATNLEIHNER"
     gene            complement(3243..3746)
                     /locus_tag="PWX02_001574"
     CDS             complement(3243..3746)
                     /locus_tag="PWX02_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDL1729570.1"
                     /translation="MVDLIVIGIIAFSVLISLWRGFTNEALSLLGWVIAFFVASSFYP
                     YLSGYLTQINSVYLQNSEFLRNGIAAAILFILTLIVCGVINGLLGKVIDLSGMSITDR
                     ILGGAFGALRGILIVSAILFFLDTFTQASQTELWKSSLLIPHFDFIVKWFFEQLQANS
                     SFLNTTK"
     gene            complement(3892..4353)
                     /gene="rnhA"
                     /locus_tag="PWX02_001575"
     CDS             complement(3892..4353)
                     /gene="rnhA"
                     /locus_tag="PWX02_001575"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HDL1729571.1"
                     /translation="MKLVEIFTDGSCLGNPGKGGIGILLRYNGYEKTVSKGYFQTTNN
                     RMELRAVIEALAMLKEPCKVQLNSDSQYMKNGIQKWIFNWKKNDWKTSDKKPVKNKDL
                     WVALDQEIQRHQIEWSWVKGHSGHRENEICDELAKQGANNPTLDDVGYIAD"
     gene            complement(4446..5777)
                     /gene="argG"
                     /locus_tag="PWX02_001576"
     CDS             complement(4446..5777)
                     /gene="argG"
                     /locus_tag="PWX02_001576"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005722481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDL1729572.1"
                     /translation="MSATILEALPLGQKVGIAFSGGLDTSAALLWMRKKGAVPYAYTA
                     NLGQPDEDDYNAIPKKAMEYGAENARLIDCRQQLAHEGIAAIQCGAFHISTGGIPYFN
                     TTPLGRAVTGTMLVAAMKEDDVNIWGDGSTFKGNDIERFYRYGLLTNPKLKIYKPWLD
                     QLFIDELGGRLEMSKFLIENGFDYKMSVEKAYSTDSNMLGATHEAKDLEELSTGMKIV
                     KPIMGVAFWDESVEIKAETVSVTFEEGVPVALNGKRIDDAVELIMEANRIGGRHGLGM
                     SDQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIEQYRINGLRLGRLLYQ
                     GRWFDPQALMLRETAQRWVAKAITGTVTLELRRGNDFTILNTESPNLTYEAERLSMEK
                     VEDAPFTPADRIGQLTMRNLDIVDTRGKLAVYTDAGLLAANNSVIPQLGKK"
     gene            5961..7001
                     /locus_tag="PWX02_001577"
     CDS             5961..7001
                     /locus_tag="PWX02_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDL1729573.1"
                     /translation="MKKLLVIRNDKIGDFMVCFPAFAMLKQSMPNVEITALVPSYTAP
                     LAELCPSIDKIIIDTANKKDKNEFNRILQAVKNEQFDAVICFVSDWHNAKLTWQSGIK
                     YRLAPATKLFQFLYNHRLTQRRSQSAKAESEYNLDLARAFLSKHNIPIVEPHTPYLSV
                     EQQTVQAQKAKLCEQLNITTVKKWVFVHSSTGGSANNLSIEQYAELIQGILAEFDCYV
                     VLTAGKGESEKAQQLAQQINHNNVVVYDKNEGLQDFTHSLACADLFIAGSTGPLHISG
                     ALNIPTIGFYPSRLSAIPRRWKPINDANKHIAFCPPSDKASQMNLTLISISEALKTIV
                     PFIRRMWNTPSL"
     gene            complement(7066..7824)
                     /locus_tag="PWX02_001578"
     CDS             complement(7066..7824)
                     /locus_tag="PWX02_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL1729574.1"
                     /translation="MPTLSVAMIVKNEAQDLRTCLDTVKDWVDEIVILDSGSTDETPQ
                     IAAEYNARFYTNTDWQGFGKQRQLAQQYVTSDYVLWLDADERVTAELKQSILQAVKND
                     EKNTVYKISRLSEVFGKQIRHSGWYPDCVVRLYRTEFAKYGDELVHEKVHYPKEAKIQ
                     KLQGDLLHFTYKDIHHYLVKSASYAKAWAIQRANAGKTASLFDGVTHAIGCFVKMYLL
                     KAGFLDGKQGFLLAVLSAHSTFVKYADLWNRTRK"
     gene            7951..8265
                     /gene="yhbY"
                     /locus_tag="PWX02_001579"
     CDS             7951..8265
                     /gene="yhbY"
                     /locus_tag="PWX02_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010786380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDL1729575.1"
                     /translation="MSITLTTKQKQFLKGLAHHLSPVVMLGGNGLTEGVLAEIDNALD
                     HHELIKVKVAGADRETKQLIIDAILRETKAAEVQTIGHILVLYRQSEEKKISLPKATK
                     AI"
     gene            8326..9048
                     /locus_tag="PWX02_001580"
     CDS             8326..9048
                     /locus_tag="PWX02_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL1729576.1"
                     /translation="MFKKFSLYTLAMLLIPVVTWLANWHWSLAIQYAEFDFDYFLYLI
                     TETGSVPYAIITCVVFALLLAWVVHKRYSLIFVITVCVASMVATQAIKTAMKSTFQEP
                     RPFVTALFQDKTDDFYQLTREQRGLAVMEKIGANQNFVTAHQAKEVGYSFPSGHTIFA
                     VSWLLLVVGFCRNMRGQAVVLAHFFAISWAALMLISRLRLGMHYPIDLFTSSLIAFLI
                     NLALFVWFVPWLESKKWFAKKP"
BASE COUNT         2767 a         1932 c         1724 g         2742 t
ORIGIN      
        1 aaaacaatca aattatattc tggttttatt ttgtcgctgt ttttgctatt cttctgttta
       61 tttttcacat tttattaacg atataaggat attcgatgaa ttggaaagca tataaaaaag
      121 ctattgcggc agctagcgtg gtagcgattg caacggtatt agtggcgtgt tcgtcttcag
      181 gccaaaaagg caaaacaacc tcttatgatg cggaaaaagc taaatttgga gcaaaatata
      241 atggtcgtca ctttatgcct tatggcacac attttcaagc tggacgttta ggattaaatg
      301 ctgctcctgt taatcaacta gactttttac agcagctttc taatgtcaga gcctatagta
      361 gcttggcaga ttcttatgct atcgcttacg gaaaaatcag tcgttggatc tcatcgggcg
      421 gtaatattaa tgatttagca aaatatggta ttagtactcg tttaatgcgt ggcgaagatg
      481 gctaccagaa tgtgttaatg acagggtatt actcgccggt tatcaaagcc cgtagaacgc
      541 ctcaggggca ataccaacac ccgatttatg cgatgccgtc tagcaaacgt ttttcacggg
      601 cggaaattta cgcaggagca ttgcaaggtc agggcttaga actggcttat agtaattcaa
      661 tgctcgataa cttcttatta ggtgttcaag gtagcggtta cgttgacttt ggtgatggtc
      721 gcttaaacta ttttgcttat gcagggcaaa atggctttaa atatgccagc gttgggcgtt
      781 tattagtaga agatggcgaa attccgaaag agaaaatgtc agtgcaggct atccgtgaat
      841 gggggcaacg taaccctagc cgtgttcaag gtttgttgga gcgtaatcca tcgtatgttt
      901 tctttaaaaa cgatcctacc ggtcaggtaa aaggctctgc cggtgtgccg ttagtaccac
      961 ttgcatctgt tgcatcagat cgtagcattg tgccatcagg tagcgtgttg ttggtcgaaa
     1021 tgccgttgat tgatagcaaa ggaaattgga ccggcaaaca cgaaatgcgt ttaatggtgg
     1081 ctcttgatgt tggcggtgca gtgaaaggcc atcacttcga tttatatcaa gggattggcg
     1141 ataaagcagg ccatatcgca ggtttaatga aacattacgg ccgtgtttgg gtattaaatt
     1201 aatacagggt aactatgtta aaaaaactat ctgccatatt cttacttcta ccgagtactt
     1261 ctaccgagcg ccgtgcttgc aaataatttg caaaatataa ccgcttcaac tgagcctaaa
     1321 tataaaatct cagaaataga tgtcagaatt ttgatacgcc agctcaataa tattgagcaa
     1381 tgtatctatc ctgagttagc taaacccggt taccagcaaa tctacgcaaa ttggaatctg
     1441 gctgaaaatt taacgatgca atattttgaa tatcagcttc taaaagagtt gctcggagag
     1501 gaaaatcaga aactgatgca aaatgatacg ctttctaccg agtatttcca tttactgcat
     1561 tctcgtttaa atcaccaaaa agcaaatgta gatcccgaaa aatgcgatac atttaaacct
     1621 agatataaag agatttataa aagtatggaa aatgcactca ctaagaaaaa tcaatagtaa
     1681 aaaactcgca gttttcactg cgagtttctt ttatctttcg ttatgaattt ctaaattggt
     1741 ggcttgtttt gccgcctctg cttttgcttt atcgtttcga gatcttgcta tactgtctaa
     1801 atagcattta tctacatcac ctgtaatata ttctcctgta aatacagagt catcaaagcg
     1861 atgaatagtc ggattttcca gttgaataga cttataaagt gcttcaaggt cttggaaaat
     1921 caatttatcc acccctatca tctgtgcaac ctcttccact gagcgatcat aagccactaa
     1981 ttcttcacaa gtcggcatat caatgccata cacattcggg tagcgaattt ccggtgcggc
     2041 agaggcaaaa taaacccgtt ttgcaccagc tgctcgtgcc atttccacga tttgttcaga
     2101 cgttgtacct cgtacaatag aatcatcaac cagtaacacg cttttgcctt taaattcaga
     2161 ggcaatcgca tttaatttac ggcgaaccga gcttttacgc tgtgcttgcc ccggcataat
     2221 aaaagttcga gctacatagc ggtttttaac aaacccttga cgatagggtt tatacaacat
     2281 attagcaata cgtaccgcaa tatcatttga ggtttcagga atcgggatca ccacatcaat
     2341 atcatcgata attcgtcccc attcacgttt aattttctca cctaataatt cgcccatatg
     2401 cactcgtgca gaatatacag aaacgccatc aatgacggaa tcaggacggg caaaataaac
     2461 atattcaaaa atacaaggat tcagttttgg attatcggca caaatttgcg aatgtaattg
     2521 cccatcaaag gtaacataaa tcgcttcacc cggcagcaca tctcgcacaa attcaaaccc
     2581 cactacatca agagccacac tttccgaagc aaacatatat tcggttttac cctcgatttc
     2641 acgtttaccc agcactaacg ggcgaatacc gaatgggtca cggaaagcaa ccataccgtg
     2701 tccgataatc atcgcaatgc aagcataagc accacgaatg ctatcattgg ttttacgaac
     2761 cgtttcaaag atattgtctg ggcttaaatg ctgagtggaa tagagatcta aaaagtaagc
     2821 aaaaatatta agaagggatt cagaatcaga attagtgttc acatggcggc gggcttcgtt
     2881 gtataagcga gctttaagtt ccgcattatt agttaaatta ccgttgtgaa ctaaggtaat
     2941 accgaaaggt gaattgacat aaaatggctg ggcttcagac acacttgagc tacctgctgt
     3001 tgggtagcga acgtgaccaa ttccaacatt gccttgtaat ctcaccatat gctcttgctg
     3061 gaaaacatcg ctgactaagc cgttagcttt gcgtaagcgg aaacgatttt catcgtctat
     3121 ggtgacgata cctgcggcat cttgtcctcg gtgttgaagt aatgttaaac catcataaat
     3181 cgcctgattc accggcgaat tcccaataat accgacaatg ccgcacatat agaaaaacct
     3241 ctttatttag tcgtgtttaa aaaactggaa tttgcttgta actgttcaaa gaaccacttc
     3301 acaataaagt caaaatgcgg aattaatagc gatgatttcc ataattccgt ttggcttgct
     3361 tgggtaaagg tatctaagaa aaataaaata gccgacacaa ttaaaatacc acgcaatgcc
     3421 ccaaaagcac cgcctaaaat acggtcggtg atagacattc cgctcaaatc aatcactttg
     3481 cctaataaac cgttgatcac accacaaaca attaaagtca aaatgaacag aatcgccgct
     3541 gcaataccat tgcgtaaaaa ttctgaattt tgcaaataaa ccgaattaat ttgtgtgaga
     3601 taaccgctta aataaggata aaacgagctt gccacaaaaa aggcgattac ccaaccaagt
     3661 agcgataatg cttcattggt aaaacctcgc cataaactga taagcactga aaaagcaatg
     3721 attccgataa caattaaatc aaccataaca caccttaaag cgtaaagctc tcctattcta
     3781 caaaaaaaga aaacgtttgc gtagcactat ttcgctaaaa ataagtgctt tatgcaaata
     3841 caagcggtta aattcggtaa aaaatttaca caatccgacc gcttgtaaaa cctagtctgc
     3901 aatataacca acatcgtcca acgttggatt atttgctcct tgtttagcca gctcatcgca
     3961 aatctcattt tcacgatgtc ctgaatgccc tttcacccaa ctccattcaa tttggtggcg
     4021 ttgaatttct tggtctaaag caacccataa atctttattt ttaactggct ttttatcgct
     4081 ggttttccag tcatttttct tccagttaaa aatccatttt tggatcccgt ttttcatata
     4141 ttggctgtca ctattgagtt gcaccttaca aggctctttt agcattgcta aagcctcaat
     4201 caccgcccgc aattccattc ggttattggt ggtttgaaaa tagcctttgc tcacggtttt
     4261 ttcgtacccg ttataacgca gcaaaatacc aatcccccct ttgccggggt tgcctaaaca
     4321 agaaccgtct gtaaaaattt caactaattt catcatcacg ctttgaaaat aaaagcccca
     4381 aagcattaac tttggggcag cttttaggct aaattctaaa agttatcaga atggagatca
     4441 actgattatt tttttccaag ttgtggaatc acgctattat tagccgccaa taaacctgca
     4501 tcggtataaa ctgctaattt accacgggta tcgacaatat ctaagttacg cattgttaat
     4561 tgaccgatac gatctgccgg cgtaaacggt gcatcttcta ctttttccat acttaaacgt
     4621 tctgcttcgt aggttaagtt tggcgattca gtgttaagaa tagtgaaatc gttaccacgg
     4681 cgtaactcaa gcgtgaccgt accggtaatc gcttttgcca cccaacgttg agccgtttca
     4741 cgcaacatta aagcttgcgg gtcaaaccag cggccttgat acaataaacg ccctaaacgt
     4801 aatccgttaa tgcgatattg ctcaatcgta tcttcgttgt gaataccggt aactaaacgc
     4861 tcgtaagcga tgtgtaataa tgccattccc ggtgcttcat aaataccacg agatttcgct
     4921 tcgataatac ggttttcgat ttggtctgac attcccaaac catgtctgcc accaatgcgg
     4981 ttagcctcca taattaattc aacggcatca tcaatacgtt taccgtttaa cgccaccggc
     5041 acaccttctt caaaggtaac agaaacagtt tccgctttaa tttcaactga ttcgtcccag
     5101 aatgccacgc ccataatcgg ttttacgatc ttcataccgg tgcttaattc ttctaaatct
     5161 ttcgcttcgt gggttgcacc taacatattc gagtctgtag agtaggcttt ttccaccgac
     5221 attttatagt caaaaccgtt ttcgattaag aatttagaca tttccaagcg accgcctaat
     5281 tcatcaatga atagttggtc taaccacggc ttataaattt ttaattttgg attggttaat
     5341 aaaccgtaac gatagaaacg ctcaatatcg ttacctttaa aggttgaacc atcgccccag
     5401 atattcacat catcttcttt cattgccgca accaacattg tgccggttac cgcacgacca
     5461 agtggcgtgg tgttgaaata cggaataccg ccggttgaaa tatggaaagc accgcattga
     5521 attgcggcaa tgccttcgtg agctaactgt tggcgacagt cgattaatcg tgcattttca
     5581 gccccatatt ccatcgcttt tttcggaatt gcgttgtaat catcttcatc cggctgacct
     5641 aaatttgcgg tgtacgcata aggcactgcc ccttttttac gcatccataa cagtgctgct
     5701 gaggtatcta aaccgcctga aaaggcgata cccactttct gccctaaagg taaagcttct
     5761 aaaattgttg ctgacatacg aattcctata aattttgaaa gtaaaaatat tcaaacgcaa
     5821 acgcttacgt tcaagcaaaa tattctagct gaaaagtagt aaaaattcat ctaaaattta
     5881 gcccgatgat ttgcaaattt tttgtgaaat ttgaccgctt ataatttact gaaaacctaa
     5941 aagcaataga gaaaatcaga atgaaaaaac tactcgtaat ccgtaatgat aaaattgggg
     6001 actttatggt ctgctttccg gcatttgcta tgttgaagca atcaatgccg aatgtggaaa
     6061 ttaccgctct tgtgccaagt tatactgctc cacttgccga gctttgccct tctattgata
     6121 agattattat tgatacggcg aataaaaaag ataaaaatga atttaaccgt attttacaag
     6181 cggttaaaaa tgagcaattt gatgcggtta tttgttttgt ttcagactgg cataatgcca
     6241 aactcacctg gcaaagtggg attaaatacc gcctcgctcc agcaactaaa ttattccaat
     6301 ttttatataa ccaccgttta acccaacgcc gttctcagtc agcaaaagcg gaatccgaat
     6361 ataatttgga tttagcccgt gcttttttaa gcaaacacaa tataccgatt gttgaacctc
     6421 acacgccata cttaagtgtt gaacagcaaa ccgtccaggc acaaaaagca aaattatgcg
     6481 aacaactcaa tattaccacc gttaaaaagt gggtatttgt gcatagttca accggtggtt
     6541 cggcaaataa tttatctata gaacagtatg cagagttaat tcaaggtatt ttggcagaat
     6601 ttgattgcta tgtggtactc accgcaggga aaggcgaaag cgaaaaagct cagcagttag
     6661 ctcaacaaat caatcacaac aacgtggtgg tttatgataa aaatgagggg ttacaagatt
     6721 ttacacattc gcttgcgtgt gcagatttgt ttattgccgg ctcaaccggt ccgttacata
     6781 ttagtggggc gttaaatatt ccaactattg gcttctaccc gagccgactt tctgccattc
     6841 cacgccgttg gaaaccgatt aatgatgcga ataaacacat tgcattttgc ccaccgtctg
     6901 ataaagcctc acaaatgaat ttaactttaa tttctatttc tgaggcatta aaaacgatag
     6961 taccgtttat tcggagaatg tggaatactc cctctctctg ataaaattgc taaacgcaat
     7021 tttatctatc tctctcccac tagagggaga gagtactgaa aagcatcatt tccgagtacg
     7081 attccacaaa tccgcatatt ttacgaaagt agaatgtgcc gaaagaactg ccaataaaaa
     7141 tccttgcttg ccatctaaaa aaccggcttt taatagatac attttcacaa aacagccgat
     7201 ggcgtgagtc acgccatcaa ataagctggc tgttttgcct gcatttgccc gttggattgc
     7261 ccacgcttta gcgtaacttg ccgattttac caaatagtgg tgaatatctt tataggtgaa
     7321 atgcagcaaa tcgccttgta gtttctggat ttttgcctct tttgggtaat gtactttttc
     7381 gtgaaccaat tcatcaccat atttggcaaa ttccgtgcga tacaatctca ccacacaatc
     7441 cggataccaa cccgagtggc gaatttgttt gccaaatact tcacttaagc ggctgatttt
     7501 gtaaacggta tttttttcat catttttgac cgcttgtaaa atagattgct tcagttcggc
     7561 tgtcactcgc tcatctgcat caagccaaag tacataatca ctggtcacat attgttgagc
     7621 taactgacgc tgtttaccaa agccttgcca atcggtgtta gtgtagaacc ttgcgttgta
     7681 ttcagcagca atttgtggtg tttcatcagt actgccacta tctaaaatca caatttcatc
     7741 tacccaatct tttactgtgt caaggcaagt ccgtaaatct tgagcttcat ttttgacaat
     7801 catcgcaaca cttaatgtcg gcatagttta tccttctctc tttttgtcgt gtgtttttct
     7861 gctattcgtt gcattttact ttaaaaatag tggtttttgt gctattttct acgccaaatt
     7921 ttatcaacaa aacaaattaa cggaaaaaga atgtcaatta cattaacaac caaacaaaaa
     7981 caattcctaa aaggcttggc tcatcattta agcccagtgg tgatgctggg gggtaacggc
     8041 ttaacagaag gcgtgttagc tgaaatcgat aacgccttag accaccacga actgattaaa
     8101 gttaaagttg ccggggctga tcgtgaaacc aaacaattaa ttattgatgc cattctgcgt
     8161 gaaactaaag cggctgaagt acaaaccatt gggcacattt tagtgcttta ccgccaaagc
     8221 gaagagaaaa aaatcagctt gccaaaagca actaaagcga tctaattgca aaaatatgtt
     8281 taaattcaac cgcttgtatt caagcggttt tttataggac tttctatgtt taaaaaattc
     8341 tctctttata cccttgcaat gttgctcatt cctgtggtga cttggctggc aaattggcac
     8401 tggtctctgg caattcaata cgctgaattt gatttcgact actttttata tttaatcacc
     8461 gaaaccggct ccgtacctta tgccatcatc acctgtgtgg ttttcgcctt attactggcg
     8521 tgggtggttc acaaacgcta ctctttaatt tttgtgatta ccgtgtgtgt cgcttcaatg
     8581 gtagccactc aagcaatcaa aacagcgatg aaatcaacct ttcaagagcc tcgtccattt
     8641 gtcactgctc ttttccaaga taaaacagac gatttttatc aattaacacg cgagcaacgt
     8701 gggcttgccg taatggaaaa aatcggtgca aatcaaaatt ttgttaccgc tcaccaagcc
     8761 aaagaagtgg gctattcttt cccatcaggc cacactattt ttgcggtttc ttggctatta
     8821 ttggtggtag gtttctgccg taatatgcga ggacaagcgg tcgttttggc tcattttttt
     8881 gcaatcagtt gggcggcgtt aatgctgatt agccgcttgc gtttaggtat gcactacccg
     8941 attgatttat tcaccagtag tttaattgcg tttctaatca atttagcctt atttgtgtgg
     9001 tttgtgcctt ggttggaaag taaaaaatgg tttgctaaaa agccttaatt ggcaatttag
     9061 ccaaatcaat atacaataca aattattgaa taagtaccta aaataaggag cttagaatgg
     9121 aaatgtcgat agaaaaaata gctgaactat taagcgataa agaaa
//
LOCUS       DANJWC010000094         9102 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.094, whole genome shotgun sequence.
ACCESSION   DANJWC010000094 DANJWC010000000
VERSION     DANJWC010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9102
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.094"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..875)
                     /gene="dusB"
                     /locus_tag="PWX02_001581"
     CDS             complement(<1..875)
                     /gene="dusB"
                     /locus_tag="PWX02_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDL1729577.1"
                     /translation="MQIGQYAIKNRIFLAPMAGITDQPFRRLCSQLGAGLTFSEMMST
                     NPDVWHTEKSRLRLAHHDEIGLNAVQIAGSDPVEMAKAAKINVEYGADIIDINMGCPA
                     KKVNKNMAGSALLRNPKLVAQILDSVVNAVDVPVTLKIRTGWDQENRNCLQIAKIAEQ
                     AGIKALTIHGRTRSCLFNGEAEYESIKAVKEAISIPVIANGDIDSAEKAKFVLDYTKA
                     DAIMIGRASFGNPWIFKEINDFFETGLSSPLSIDKKYQLMFKHIEDLHQFYGEEKGYR
                     IARKHVGWYTEKLNP"
     gene            complement(1099..1587)
                     /gene="sixA"
                     /locus_tag="PWX02_001582"
     CDS             complement(1099..1587)
                     /gene="sixA"
                     /locus_tag="PWX02_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HDL1729578.1"
                     /translation="MNIWIMRHGEAGFNASSDAARCLTDNGIKNTISQGKWLGEHLAN
                     QQIQLDKILVSPYLRAQQTLENVLIGMQAVNFPQHFTNLIESWDEVTPDGNPHTVESY
                     LHFLREEGAKNILLISHLPLVFDLVQILTNNQSNVQFPTSTIAEINWVKTKAELIRVK
                     HT"
     gene            1714..3153
                     /gene="pyk"
                     /locus_tag="PWX02_001583"
     CDS             1714..3153
                     /gene="pyk"
                     /locus_tag="PWX02_001583"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL1729579.1"
                     /translation="MSRKLRRTKIVCTMGPATDRGNTLEKIIAAGANMVRMNFSHGIP
                     EDHIERANKVREIAARLGRHVAILGDLQGPKIRVSTFKDGKIFLNIGDKFTLDADLPR
                     GEGHQEAVGLDYKNLPNDVVPGDILLLDDGNVQLKVLEVEGVKVHTEVTVGGLLSNNK
                     GINKLGGGLSAPALTDKDKEDIKLAAKIGVDYLAVSFPQSSADLHYARELAKEAGLEA
                     KIVAKVERAETVATEEAMDDIILGADVVMVARGDLGVEIGDAALVGVQKRLIRRARKL
                     NRVVITATQMMESMIKKPMPTRAEVMDVANAVLDGTDAVMLSGETANGDYPVETVKSM
                     AEVCLGAETMPSINISRHRMEGTFKSIEEAVAMSAMYTANHLEGVSAIISLTHSGETA
                     KLTSRISSGLPIYAMSRNQKALNLCALYRGVTPVYYNEDSRTMEGAKKAIAQLKEQGY
                     LLTGELVLLTHGDELKAGGTNTCRILRVE"
     gene            3249..4286
                     /locus_tag="PWX02_001584"
     CDS             3249..4286
                     /locus_tag="PWX02_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDL1729580.1"
                     /translation="MHNKLLSICILRLSAIGDVCHTLAVIQAIQRKYPSAEITWIIGK
                     TEAYLMKDIPNVHLIPYDKKIGWKGIFTLWQKLSHKRFDFLLNLQTAFRASILSLGIK
                     AKKKIGFNQDRAREMQWLFTNQKVEMTDSPHVLDGQMMFAKAIGVMDLSAQWNLPVSD
                     EDLAYGKQFIDFTRKNILIAPCSSKKEKDWLPERYAEIANWLSKQNINVLIAGSPSQY
                     EMETAAKIQQLAPNCTSIAGKTTLKQLAGVIRQVDLVISSDSGPAHIATTQNTPVIGL
                     YAIHNPRRTGPYNDLDKVVSVYDEAILQSYGKPWQQLAWATKAKGKNWMEKITVESVK
                     QKVVETLKITL"
     gene            4326..5384
                     /locus_tag="PWX02_001585"
     CDS             4326..5384
                     /locus_tag="PWX02_001585"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDL1729581.1"
                     /translation="MNTVFNQDSVHNVNIQDEKVLLTPQELKQEFPLPEHLRNQIERS
                     RKTISDIIHRRDKRQLIVIGPCSIHDPVAALEYGKKLKALSEQVSDKLYIVMRVYFEK
                     PRTTVGWKGLINDPKMDGSFDVETGLRIARKLCLDLAELGLPLATEALDPMTPQYLAD
                     LFSWSAIGARTTESQTHRELASGLSMAVGFKNGTDGDLAVALNAMQSSAQSHSFIGIN
                     QQGQVTLLKTKGNADGHIILRGGKTPNYEKQYVDDCEWVLKQANLPTAIMIDCSHGNS
                     NKDYRRQPLMAENVLEQLLAGNTSIIGLMIESNLHAGNQSSELPFEQMKYGVSITDAC
                     IDWQTTEELLTHFAEKLR"
     gene            5439..6560
                     /gene="tyrA"
                     /locus_tag="PWX02_001586"
     CDS             5439..6560
                     /gene="tyrA"
                     /locus_tag="PWX02_001586"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HDL1729582.1"
                     /translation="MNPLAPLREQIDQVDQQLIALLSERLALVAEVGKVKSEHGIPVY
                     APERETAMISARRSEAEKQGIPADLIEDVLRRIMRESYANENKHGFKRVNSDIQKIVI
                     VGGKGKLGGLFGNYLTLSGYNVEALGRDDWDNAEQILANADVVIVCVPIANTLATIER
                     LQSYLTENMILADLTSVKHQPLQKMLEVHNGAVVGLHPMFGPDIASQAKQVVACCHGR
                     FPERYQWLLEQIKIWGAKIEEIEAAAHDNAMTYIQALRHFSTFAFGLHLSRQPVQLSQ
                     LLALSSPIYRLELAMIGRLFAQDGSLYADIIADKPENLATIETLKESFEQGLDFFKRN
                     DKAGFIKAFEEVHHWFGDYSEQFLKESRVLLQQAHDSRK"
     gene            complement(6751..7836)
                     /gene="recF"
                     /locus_tag="PWX02_001587"
     CDS             complement(6751..7836)
                     /gene="recF"
                     /locus_tag="PWX02_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDL1729583.1"
                     /translation="MALTRLLINHFRNIQHTDLAFSPHFNFLVGANGSGKTSLLESIF
                     YLGHGRSFKSHISNRIIHYDKDDFVLHGKIDEAKHSWSVGIQKFRSGETTLKINGEDG
                     NKIADLAHLLPMQVITPEGLTLLNGGPSFRRAFLDWGLFHQHPDFYAHWNNLRRLLKQ
                     RNSALQQVRSYQELKAWDIELVKTTYAVSEMRAEYAEALRPEIEKTCQFFLPEIEIGL
                     SFHQGWEKGADYAEILAQGFERDKALGYTMIGAQKADFRFKANGLPVEDVLSRGQLKL
                     LMCALRLAQGEHLVAQKQRQCLFLIDDFASELDPTKRELLAHRLRESGSQVFVTAITQ
                     DQLNQMQWQDRSDDCLFTVKNGEIQPQ"
     gene            7976..8305
                     /locus_tag="PWX02_001588"
     CDS             7976..8305
                     /locus_tag="PWX02_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDL1729584.1"
                     /translation="MFGKGGLGGLMKQAQQMQERMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRIEIDPSLMEDDKEMVEDLVAAAFNDAVRRAEEMQKEKMASVTAGMQLP
                     PGMKFPF"
     gene            8313..8918
                     /gene="recR"
                     /locus_tag="PWX02_001589"
     CDS             8313..8918
                     /gene="recR"
                     /locus_tag="PWX02_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDL1729585.1"
                     /translation="MQTSPLLENLMEALRALPGVGPKSAQRMAYHLLQRNRSGGVALS
                     KALNEAMSHIGHCKSCRTFTEEEECTICKNPRRQMSGQLCVVEMPEDIQAIEQTGQFS
                     GRYFVLMGHLSPIDGIGPREIGLDLLQQRLEQESFHEVILATNPTIEGDATANYIAEM
                     CRMYNVKATRIAHGVPVGGSLEMVDGTTLSHSFAGRRDIDI"
     gene            8990..>9102
                     /locus_tag="PWX02_001590"
     CDS             8990..>9102
                     /locus_tag="PWX02_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005722395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HDL1729586.1"
                     /translation="METIDKIKQQISENPILLYMKGSPKFPSCGFSARAVEA"
BASE COUNT         2745 a         1735 c         1974 g         2648 t
ORIGIN      
        1 tccggattta atttttcggt ataccaacca acatgttttc gtgcaatacg ataacctttt
       61 tcttcaccat aaaattggtg caaatcctca atatgtttaa acattaattg gtatttttta
      121 tcaattgata atggtgaaga taatccagtt tcaaaaaaat cattaatttc cttaaatatc
      181 caaggatttc caaagctagc gcgcccaatc atgattgcat cggctttggt atagtcgaga
      241 acaaactttg ctttttccgc agagtcaatg tctccgtttg ctatcactgg gatagaaatt
      301 gcttctttta ctgccttaat actttcatat tcagcttcgc cattaaataa acagcttcga
      361 gtacgcccgt gaatagtgag agcttttatg ccggcttgtt ctgcaatttt cgcaatttgc
      421 agacaatttc tattttcctg atcccaacct gttctaattt ttagtgtcac agggacatca
      481 acggcattga ctacagaatc taatatttga gcgactaatt tggggttacg taaaagagca
      541 gagcctgcca tatttttatt cacctttttt gccgggcaac ccatattaat atcaataata
      601 tcggcaccat actcaacatt tatctttgca gcctttgcca tttcaacagg atcagaacct
      661 gcaatttgta cggcatttaa gcctatttca tcgtggtgtg ctaaccgcaa ccgtgatttc
      721 tcagtatgcc atacatcagg attggtagac atcatctctg aaaatgttaa acctgctcca
      781 agttggctac ataagcggcg aaaaggctga tctgtgattc cagccatagg tgcaaggaaa
      841 atacgatttt tgattgcata ttgtcctatt tgcattttta accttttatt aacaaatttg
      901 aagtaatttt aaccagaggg atatccaaat aaaaagtacg gtaaataccg tactttctta
      961 taatagctta tggatcagtt tagtactgat ttgagggctg gtatgatacg cattttttag
     1021 gtattttgga agtctataaa ttcgacaatt tataaaaaaa ttgaaaattt ttatattgac
     1081 attctaaaaa tgaaagaatt aagtatgctt tactcgaatt agctcagctt ttgtctttac
     1141 ccaattaatt tctgctatgg ttgaagttgg aaattgtaca ttgctttggt tattcgttaa
     1201 aatttggacg agatcgaaca caagtgggag atgtgaaatc agaagaatat tttttgcgcc
     1261 ttcctctctt aaaaaatgta gatagctttc tactgtatgc gggtttccgt caggcgtaac
     1321 ctcgtcccaa ctttcaatga ggttggtaaa atgttgggga aaattgaccg cttgcatacc
     1381 aataagcaca ttttcaagcg tttgttgcgc cctaaggtat gggctgacga gaattttatc
     1441 taattgaatt tgttggttgg ctaaatgttc tcctaaccat tttccctgtg aaatggtgtt
     1501 ttttatacca ttgtcggtta aacagcgagc agcatcactc gaagcattaa agcctgcttc
     1561 cccatggcgc ataatccaaa tattcataac attctccttt agttgtattt aaactctctt
     1621 gatttaggcg tataatgacc ctcgtttaac gcataatcaa gatgcttaga aagattttaa
     1681 cttaattttt taatttattg atggagtcaa ataatgtcta gaaaactcag aagaacgaag
     1741 atcgtttgta caatgggacc tgcaacagat cgtggcaata cgttggaaaa aatcattgct
     1801 gccggtgcaa atatggttcg tatgaatttc tctcacggta ttcctgaaga tcacatcgaa
     1861 cgtgcgaata aagtgcgtga aattgctgca agattaggtc gccacgttgc gattttaggc
     1921 gatttgcaag gtcctaaaat tcgtgtttcg acatttaaag acggtaaaat tttcttaaat
     1981 attggtgata aattcacctt agacgcagat ttaccgcgtg gagaaggcca tcaagaagct
     2041 gtaggtttag attacaaaaa cttaccaaat gatgtagtac ctggtgatat tctattgtta
     2101 gatgacggta atgttcaact gaaagtgttg gaagtggaag gcgttaaagt tcatactgaa
     2161 gtgacagttg gtggcctgtt atcaaacaat aaagggatta acaaattagg tggtggttta
     2221 tcagctcctg cattaaccga caaagataaa gaagacatta aattagcggc gaaaatcggt
     2281 gtggattacc tagcggtttc attccctcaa tcaagtgcag atttacatta tgcacgtgaa
     2341 ttagcaaaag aagcaggctt agaagcgaaa attgtagcga aggttgaacg tgccgaaact
     2401 gttgcgactg aagaagcaat ggacgacatc attttaggtg cggacgttgt gatggttgct
     2461 cgtggtgact taggggttga aattggtgat gcggctttag tgggtgtgca aaaacgttta
     2521 atccgccgtg ctcgtaagct aaatcgtgtg gtaattaccg caacccaaat gatggaatcg
     2581 atgattaaaa aaccaatgcc gacccgtgct gaagtgatgg atgttgcaaa tgctgtgtta
     2641 gatggtactg atgcggtaat gctttccggc gaaactgcaa acggtgacta ccctgttgaa
     2701 acggtaaaat caatggcaga agtgtgctta ggtgctgaaa caatgccaag cattaacatt
     2761 tctcgccacc gtatggaagg tacgtttaaa tctatcgaag aagctgtggc aatgtctgca
     2821 atgtacaccg caaaccattt agaaggtgta tcggcaatta tttcattaac tcattcaggc
     2881 gaaacggcta aattaaccag ccgtatcagc tcaggtttac cgatttatgc gatgtctcgt
     2941 aaccaaaaag cattaaacct ttgtgcgtta tatcgtggtg taacaccggt atattacaat
     3001 gaagacagcc gcacaatgga aggggcgaaa aaagccattg ctcaattaaa agaacaaggc
     3061 tatttattaa ccggcgaatt agtgctttta actcacggag atgaattaaa agctggcggt
     3121 acaaatactt gtcgtatctt aagagttgaa taatctaaac gattaacgca aaagccacat
     3181 aaaacacttt atgtggcttt ttttctgtct taatatagct taatgtaatc tgaactattg
     3241 atattattat gcacaataaa cttctttcta tctgtattct ccgcctttct gccattggcg
     3301 acgtttgcca taccttagcg gttattcaag ctattcaacg aaaatatcct agtgctgaaa
     3361 ttacttggat tatcggcaaa acggaagcgt atttaatgaa agatattccg aatgttcact
     3421 taattcctta tgataaaaaa atagggtgga aaggtatctt tacactttgg caaaaacttt
     3481 ctcacaagcg gtttgatttt ttattaaatt tacaaacggc attccgagct tcaattctat
     3541 cgcttgggat taaagcaaag aaaaaaatcg gctttaatca agacagagca agggaaatgc
     3601 aatggttgtt taccaaccag aaagtagaaa tgacagactc tcctcacgtg cttgacgggc
     3661 aaatgatgtt tgccaaagcc attggggtaa tggatttatc ggcacaatgg aatttacctg
     3721 tgagtgatga agatttagct tatggcaaac aatttattga ttttacaagg aaaaatatac
     3781 tcattgctcc ttgttcaagc aagaaagaga aagattggtt gccggaacgc tatgcagaaa
     3841 ttgctaactg gctaagtaag caaaatatca atgtattaat tgcaggttct ccatcgcaat
     3901 atgaaatgga aaccgcagcc aaaattcaac aacttgcccc gaattgtacg agtattgccg
     3961 gtaaaaccac gctaaaacag cttgcaggtg ttattcgtca ggttgatttg gtgatctctt
     4021 cagattcagg tcctgcccat atcgccacta cgcaaaatac gccggtgatt gggttgtatg
     4081 cgattcataa tccaagacgg acaggccctt acaatgactt agacaaagtg gtttcggtat
     4141 atgatgaagc gattttgcaa tcttatggta aaccttggca gcaattagct tgggcaacta
     4201 aagctaaagg caaaaattgg atggaaaaaa tcacggtgga gagtgtgaaa caaaaagtag
     4261 ttgaaacttt aaaaattacg ttgtaaagtt tgtgtattaa aagatttaca caaaaggaaa
     4321 ttaaaatgaa cacagtgttt aatcaagata gcgtgcataa cgtaaatatc caagatgaaa
     4381 aggtattact gaccccacaa gagttaaaac aagaatttcc gttgccggaa catttacgca
     4441 atcaaattga acgttcacgc aaaacgattt ccgatattat tcacagacga gataaacgcc
     4501 aactgattgt gattggacct tgttcgattc acgacccagt tgctgccctt gaatatggta
     4561 aaaaattaaa agctctttcg gagcaagtga gtgataagtt gtatattgtg atgcgggttt
     4621 attttgaaaa gccccgcact actgtaggtt ggaaagggct gattaacgat ccgaaaatgg
     4681 acggctcttt tgatgtcgaa accggcttac gtatcgcacg aaaactctgt ttggatttag
     4741 ccgaacttgg cttgccgtta gcgaccgaag ccttagatcc gatgacacca caatatttag
     4801 cggatttgtt cagctggtcg gcaattggag caagaacaac tgagtctcaa actcaccgtg
     4861 aattagcctc aggtttgtca atggcggtag gctttaaaaa tggtacagat ggtgatttag
     4921 ccgttgcact taatgcgatg caatcttccg ctcaaagcca tagctttatc ggcattaacc
     4981 aacaggggca agttacttta ctcaaaacaa aaggtaatgc agacggacat attattttac
     5041 gtggcggtaa aaccccaaat tatgaaaagc aatatgttga tgattgtgaa tgggtgctaa
     5101 aacaagcaaa cttacctaca gcgattatga ttgattgcag ccacggcaat tccaataaag
     5161 attaccgccg ccaaccgctg atggcagaaa atgtattaga gcagctactt gctggcaata
     5221 cttcgattat cggtttaatg attgaaagca atttacacgc aggtaatcaa tcatcagaat
     5281 tgccgttcga gcagatgaaa tacggtgttt ctattacgga tgcttgtatt gactggcaaa
     5341 ctacagaaga gcttttaact cactttgctg aaaagttgcg ttaaagataa tttgcaaaaa
     5401 attgcataaa aacgaccgct tgtaaattgg taacaaatat gaacccatta gccccattac
     5461 gagaacaaat tgatcaagtt gatcaacaac ttatcgcctt actttctgaa cgcttagctc
     5521 ttgtggcaga agttgggaaa gtcaaatcag aacacggcat tcctgtttat gctccggagc
     5581 gagaaaccgc aatgatttcc gcccgccgct ctgaagcgga aaaacaaggt attccggcgg
     5641 atctcattga agatgtgcta cgccgtataa tgcgtgaatc ttatgccaat gaaaacaaac
     5701 acggcttcaa aagagttaat tcggacattc aaaaaatcgt gattgttggt ggtaaaggga
     5761 aattaggtgg gctgtttggc aattatttaa ccctatccgg ctataatgtt gaggcattag
     5821 gacgtgatga ttgggacaat gccgagcaga ttttagccaa tgcagatgtg gttattgtgt
     5881 gtgtgccgat tgccaacacc ctagcaacca tcgagcgttt acaatcttat ttaaccgaaa
     5941 atatgatttt ggcggattta acctccgtca aacatcagcc gttgcaaaaa atgctggaag
     6001 tgcataatgg agcggtagtc ggtttacacc cgatgttcgg accggatatt gcaagtcagg
     6061 caaagcaagt ggtcgcttgc tgccacgggc gtttccctga acgttatcaa tggttgcttg
     6121 agcaaatcaa aatttggggg gcaaaaattg aagagattga ggcagcagca cacgataatg
     6181 caatgaccta cattcaagcc ctacgccact tttccacttt tgcattcggg ctacatcttt
     6241 ctcgccagcc ggtgcaactc tctcaattat tggcactatc ttcgccgatt taccgcttag
     6301 agttggcaat gattgggcgt ttatttgccc aagacggcag cttatatgcc gatattattg
     6361 ccgataaacc ggaaaattta gccaccatcg aaaccttaaa agagagtttt gaacaagggc
     6421 tagatttctt taaacgcaac gacaaagccg gttttatcaa ggcttttgag gaagtgcatc
     6481 actggtttgg cgattattcc gagcagttct tgaaagagag tagagtattg ctccaacagg
     6541 ctcacgattc aagaaagtaa aggtaggttg aggttttgct ctgatagcac tagcgtccac
     6601 gctagtgcta tttaatattc tacaagcggt taataaagag ctgttttttg caaattttta
     6661 tgcaaaatta tccgcttgtt ttggcacgag cgtggacgct cgcgccatcg gaagaaacat
     6721 ttttgtaaaa ttgcccccaa atctgaccgc ttactgtggc tgaatttctc cattttttac
     6781 cgtaaacaaa caatcatcgc ttctgtcttg ccattgcatt tgattgagct gatcttgagt
     6841 aatggcggta acgaatactt gtgagccact ttctcttaaa cgatgtgcta aaagctcacg
     6901 tttggttggg tcgagttcgg aggcaaaatc gtcaattaaa aataggcatt ggcgttgttt
     6961 ttgggcgacc aaatgttcgc cttgagccag tcttaacgca cacatcaata gctttaattg
     7021 cccacgggaa agcacatctt ccaccggtaa gccgttcgct ttaaaacgga aatcggcttt
     7081 ttgagctcca atcatcgtat agccaagagc tttatcccgc tcaaaacctt gtgccagaat
     7141 ctcggcataa tccgcccctt tttcccagcc ttgatgaaag ctcaagccga tttcgatttc
     7201 cggcaggaaa aattggcagg ttttctcaat ttcggggcgt aacgcttcgg cgtattcggc
     7261 tcgcatttcg ctcacggcat aagtggtttt caccaattcg atatcccacg ctttgagttc
     7321 ttgataagag cggacttgtt gcaaagccga attacgttgc tttaataaac ggcgaagatt
     7381 gttccaatga gcgtagaaat cagggtgctg gtgaaataag ccccagtcca aaaaagcacg
     7441 gcgaaagctc ggaccaccgt taagtaaggt taagccttcg ggggtgatca cttgcatcgg
     7501 caataaatgg gctaaatcgg caattttgtt gccgtcttca ccgttgattt taagtgtcgt
     7561 ctcgccactg cgaaatttct gaatgccgac cgaccaactg tgctttgcct cgtcaatttt
     7621 accgtgtagt acgaaatcgt ctttatcgta atggataatg cggttgctga tatggctttt
     7681 gaatgaacgc ccgtgtccaa gatagaaaat tgattccaac aaactggttt tgccacttcc
     7741 gttcgccccg actaaaaaat taaaatgcgg gctaaatgct aaatcggtat gttggatatt
     7801 tctaaaatgg ttgattaata aacgggttaa agccatagtt aaagtgcgag gaataaaaag
     7861 agtagccatt ttagcaaatt tgcaaaattt tgggaaaaat taaccgcttg cggattagaa
     7921 agtggttcag tagaataagc cgtttatttt ttattgaaaa ttaagaggaa aaattatgtt
     7981 tggaaaaggt ggcttaggcg gcttaatgaa acaggctcag caaatgcaag agcgtatgca
     8041 aaaaatgcaa gaagagatcg cccaattaga agtaacaggc gagtctggtg cgggtttggt
     8101 aaaagtcact attaacgggg ctcacaattg ccgccgaatt gaaattgatc catcattaat
     8161 ggaagatgat aaagagatgg tggaagattt agttgccgct gcctttaacg atgcggttcg
     8221 ccgtgcagaa gaaatgcaaa aagagaaaat ggcaagtgtg actgccggta tgcaactgcc
     8281 gccgggtatg aaattcccat tctaatttaa gtatgcaaac cagtccttta cttgaaaatt
     8341 tgatggaagc cttgcgtgca ttgcccggtg tcggaccgaa atcggcacag cgaatggctt
     8401 accatctgtt gcaacgtaat cgttccggcg gtgtggcgtt gtcaaaagcc ttaaatgagg
     8461 caatgagcca tatcggacat tgcaaatcgt gccgtacttt taccgaagag gaagagtgta
     8521 cgatttgtaa aaatcctcgc cgtcagatga gcgggcaact ttgtgtggta gaaatgccgg
     8581 aagacattca agcgattgag caaaccgggc aattttcagg gcgttatttt gtgctgatgg
     8641 ggcatttgtc gccgattgat ggtataggcc ctcgtgaaat cggcttagat ttattgcaac
     8701 aacgcttgga acaggaatct ttccacgaag tgattttggc gactaatccg accattgaag
     8761 gtgatgctac cgcaaattac attgctgaaa tgtgcagaat gtataacgtg aaagctactc
     8821 gtattgctca cggtgtgccg gtcggtggtt cattagaaat ggttgatggc acaacgcttt
     8881 ctcactcttt tgcaggacgg cgtgatattg atatttaaag ctatttctgt gctaacttaa
     8941 ttctgatata attactcaac tattttaaat tattattttg aggctaaaaa tggaaactat
     9001 tgacaaaatc aaacaacaaa tcagcgaaaa cccgattcta ctttatatga aaggttcacc
     9061 gaaattccca tcttgtggtt tctccgctcg tgcggtggaa gc
//
LOCUS       DANJWC010000095         9051 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.095, whole genome shotgun sequence.
ACCESSION   DANJWC010000095 DANJWC010000000
VERSION     DANJWC010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9051
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.095"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..1414
                     /locus_tag="PWX02_001591"
     CDS             <1..1414
                     /locus_tag="PWX02_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription antiterminator BglG"
                     /protein_id="HDL1729587.1"
                     /translation="IMLTVRLYKYFTQLEHPFGIKEFEQCFACPMTEKIRERLAKTII
                     HFNLVFSDIYAERFIIYLRYFYNQQPELLLPKLNFNYQQTNEYQFVIKLLEQLREQDS
                     RFEFNPEVIRFLTYIAIASTDLYRFKDCNSDNYPLLLDKAWEIHRFIFTQFSAYLNLS
                     EIHDDTCRKDLLKMLIPIAMKMMLSISDDVDLGLFNQTELHRNPILAHTVEKLVDDIE
                     KNYGYSLSIREQYFIFDIFSGMVNRIELERKPLRLAIIALNGRLSTQQLKRNIKRYFS
                     DYILKIETKVLYELAIMEDKPYDYYLCNEYGKNMAIPYKPIYFADSSLNENEYATSLN
                     EVFYHAYQYDEVLPSIKSIEIEAQYRLNEFHLEQEYLNYEQIILENKQDKIKLFYQLN
                     ASVEKFEIYYFKNKEDFTLYGYTKFIVMSLNVIEQPQKLKMILNIINKIAQTNFILNQ
                     LCWLDVKSYKYFFTQYKEKS"
     gene            1414..3561
                     /locus_tag="PWX02_001592"
     CDS             1414..3561
                     /locus_tag="PWX02_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL1729588.1"
                     /translation="MKRLRYSYLALILGSALPLSAYADGYLSNDNIRQFDARFNFFLA
                     NPLPAPIAKADGRGEAIENLVVKYASDYLNDIYLKEDVIRFRRWQWQDLYTSLDLKTK
                     QKPDYLNTYRLQAEAYFINKQYQEALSQLDQILRRNPNDVHAMAMSVVASRVLENPNT
                     EAERLLALYQISPSIAQKVQQYLHFTDTMLAASYGSEPQTTMIPDTIAVFGQSPNPDG
                     TPSKGLLQRLEKTKEMAAKFPNAKIILSGGPVRYIYAEADVMKKWLIENGVDESRLLL
                     DDVARDTPGNAVGIIDFMKEYGGRKVLGIGTILHLPRAMSVLKSYADSIGYELEIDSA
                     GGGSPPNEKNKKGEALYTFVNVARAMGLFTLGDFENLLAKEQAEKEQREALEKAQQNY
                     LEKLVAERDQAVKNAENSANELEVARQALETAKQAHQQAEEELNKELSKTQEQLKQST
                     EALSRLIIKQNNPFSEHYVVQLNLMEQATYDLYRRLAEIEHTQDWNLWLSDSLSKYRL
                     KNRSTEDYHLLHIGLDHQVSHNWSIGGVFGISQGQIENRTHKVKGTSAGIYAGYRPTS
                     GLFWTNFFNYSHFTNQSGVTKRDNNAYSLSSEIGRNIVMSHQWQITPKLQLGWAKAGS
                     ARMFWAGTGLRLGKVIELNAIKLKPYADISYRFRNRHIDTLDSLSDRHRNVISIGVQA
                     VGNRHYFAIEGSMLHGDALKRSYGLHANYHYQW"
     gene            3574..4692
                     /locus_tag="PWX02_001593"
     CDS             3574..4692
                     /locus_tag="PWX02_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL1729589.1"
                     /translation="MKKIKTTLSALALSLSLISVNSWAIPADYLSQDNISQYDKAFNF
                     VLAKVLPAPKGKEDGRGAPIEHLVIQYAPDYVDDPYLKEDVLNFRRWYWQAMYASLDQ
                     KVKEKPTYLNTYRLQAEAYFVNKEYREALSQLDQILRQNPRDVHALAMTVLASRVLED
                     TEQETARLSALKQLAPEVAQRMTDFFAFTEQTLAASYGNEPQTAMIPDTIAVFGSTPN
                     KDGTPSPLLLDRLQKTKEMAQKFPQAKIIVSGGAVKTEFAEADVMAKWLSENGIEKDR
                     LLLDPVARDTPGNAVGIIKLMQTYNGKKVLGIGTLQHIPRATAVLKGYAQHIGYPIEM
                     DSVGGGKPATPGKAKIEALYTYVNVARSMGLFELGDFK"
     gene            complement(4753..6015)
                     /gene="rho"
                     /locus_tag="PWX02_001594"
     CDS             complement(4753..6015)
                     /gene="rho"
                     /locus_tag="PWX02_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDL1729590.1"
                     /translation="MHLTQLKNTPVSELVEMGENQMGLENLARLRKQDIVFAILKQHA
                     KSGEDIFGQGVLEILPDGFGFLRSSDSSYLAGPDDIYVSPSQIRRFNLQTGDKIEGKI
                     RPPKEGERYFALLKVDSVNDDKPEASRSKILFENLTPLHANSRLKMERGNGSKEDLTA
                     RILDLAAPIGKGQRALIVAPPKAGKTVLLQNIAQSITHNHPECELIVLLIDERPEEVT
                     EMQRTVRGEVIASTFDEPATRHVQVAEMVIEKAKRSVEHKKDVVILLDSITRLARAYN
                     TITPVSGKILSGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVDTGSKMDEVIFE
                     EFKGTGNMELHLSRRIAERRIFPAIDFNRSGTRKDDLLMAADELQKTWMLRKVLNPMD
                     EVDAIEWLIDKLMMAKTNEEFFEIMKRS"
     gene            complement(6201..7148)
                     /gene="cysK"
                     /locus_tag="PWX02_001595"
     CDS             complement(6201..7148)
                     /gene="cysK"
                     /locus_tag="PWX02_001595"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDL1729591.1"
                     /translation="MTIYADNSYSIGNTPLVRLKNFGNGNIVVKVEGRNPSFSVKCRI
                     GANMVWQAEKDGILTKDKEIVDATSGNTGIALAYVAAARGYKLTLTMPETMSTERKRL
                     LRGLGVNLVLTEGAKGMKGAIAKAEEIASSDPERYVTLKQFENPANPAIHQQTTGPEI
                     WDATEGKIDVLVAGVGTGGTITGVSRAIKQDKGKQIISVAVEPSESPVITQTLNGEEV
                     KPGPHKIQGIGAGFIPKNLDLSLLDRVEQVSSEEAITTARRLMAEEGILGGISSGAAV
                     AAADRIAKLPEFADKLIVVILPSASERYLSMLLFEGIEA"
     gene            complement(7322..8818)
                     /gene="pepA"
                     /locus_tag="PWX02_001596"
     CDS             complement(7322..8818)
                     /gene="pepA"
                     /locus_tag="PWX02_001596"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDL1729592.1"
                     /translation="MEFSVKNGSVEKQRTACLVVGVYEPRRLCSAAEQLDKLSNGYIS
                     TLLRRGDLEGKAGQTLLLHNVPNVLADRVLLVGCGKERELTERQYKQIIQKSVQTINE
                     TGSMEAVSFLTELHVKGRTPYWNVRFAVEAIQEQLYAYNDFKSIKPEVRRELRRVIFN
                     VAHRKDLPDAERALEHGKAVALGISHAKNVANCPPNVCNPHYLAELAKGLAYDYPAIS
                     TTIIDEKQMAELGMNAYLAVSRASENEAFLSVIEYKGNPNADAKPIILVGKGMTFDSG
                     GLSIKPAEAMDEMKYDMCGAATVYGTIKAIAEMKLPLNVIGLLAGCENAVDGKAYRPG
                     DILTTMSGLTVEVLNTDAEGRLVLCDALSYVERFEPELVIDIATLTGACMIALGNHNS
                     GLMSPHNGLANELLAAAEQADDKAWRLPLGEEYQEQLKSNFADLANIGGRLGGACTAG
                     QFLSNFTKKYTWAHLDIAGTAWKSGAAKGATGRPVSLLTQFLINKAQV"
BASE COUNT         2692 a         1731 c         1864 g         2764 t
ORIGIN      
        1 aataatgctc acagtacgat tatataaata ttttacccag cttgaacatc catttggaat
       61 taaagaattt gaacagtgtt ttgcttgtcc tatgactgaa aaaatccgtg aaaggttagc
      121 taagacaatt attcatttca atttagtatt ttctgatatt tatgctgagc ggtttattat
      181 ttacttacgc tatttttata atcaacagcc tgagttattg ttaccgaagt taaattttaa
      241 ttatcaacag acgaatgaat atcagtttgt aattaagtta ctggaacagc ttcgtgaaca
      301 agacagtcgt tttgagttta atccggaagt tattcgattt ctgacctata ttgcgatagc
      361 cagtacagat ctctaccgct ttaaagattg taattcggat aattacccat tgttgctaga
      421 taaggcttgg gaaattcatc gttttatttt tactcagttt tctgcatatt taaatttatc
      481 ggaaattcac gatgacactt gccgtaaaga tttgcttaaa atgctgatcc ctatcgcaat
      541 gaaaatgatg cttagcattt cagatgatgt ggatttaggg ctatttaacc aaacagaact
      601 acatagaaat ccaattttgg ctcatacagt agaaaaatta gtggatgaca ttgaaaaaaa
      661 ctacgggtat agcctatcta ttcgagaaca gtattttatt tttgatattt tttccggaat
      721 ggttaatcga attgaattag aacgtaaacc gctccgactg gcgattattg cccttaatgg
      781 cagattaagc acccaacagt taaaacggaa tattaagcgg tacttttccg actatatttt
      841 gaaaattgaa accaaagtgc tatatgagct tgccataatg gaagataaac cttatgacta
      901 ctatctctgt aatgaatatg gaaaaaatat ggctattccc tataagccta tttattttgc
      961 tgacagtagt ttaaatgaaa atgaatatgc aacatcactc aacgaagtat tttatcacgc
     1021 ttatcaatat gatgaggtgt tgcctagcat taaatcgatt gaaattgaag cacaatatcg
     1081 cctaaatgag tttcatcttg agcaagaata tcttaattac gaacagatta ttttagaaaa
     1141 taaacaagac aagattaaac tattttatca actcaatgcc tcggtagaaa aatttgagat
     1201 ctattatttt aaaaataaag aagatttcac tttatatggt tatactaaat ttattgtgat
     1261 gagtcttaat gtcattgagc aaccacaaaa attaaaaatg attttgaata ttattaataa
     1321 aatagcacaa acaaatttta tccttaatca actttgttgg ttagatgtaa aaagttataa
     1381 atacttcttc acccaatata aggagaaatc ataatgaaac gcttgcgtta ttcttatctt
     1441 gctttgatat taggttcagc cttaccttta tcggcttatg ctgacggtta tttaagtaac
     1501 gataatattc ggcaatttga tgcccgtttt aattttttcc tcgcaaatcc actacctgcg
     1561 cctattgcaa aagcagatgg gcgtggtgaa gcgattgaga atttggtggt gaaatatgca
     1621 tcagattatc ttaatgatat ctatcttaaa gaggatgtta ttcgttttcg ccgttggcag
     1681 tggcaagatc tctatacgtc attggattta aaaacgaaac aaaagcccga ttatttaaat
     1741 acttaccgat tacaggcgga agcgtacttt atcaataagc aataccaaga agcattatct
     1801 caactggatc aaattctgcg gcgtaaccct aatgatgttc atgcaatggc aatgagtgtg
     1861 gttgcaagtc gggtattgga aaatcctaat actgaagccg agcgtttatt agcgctttat
     1921 caaatctctc caagtattgc gcaaaaggtg cagcaatatc tacattttac agacactatg
     1981 cttgctgcca gctatggtag tgagcctcaa acaacaatga tccccgacac catagcggta
     2041 tttgggcaat cgcctaaccc agacggtact ccaagcaagg gattattgca acgtcttgag
     2101 aaaaccaaag aaatggcagc gaaatttccg aatgcaaaaa ttattttaag tggtggacct
     2161 gttcgttata tctatgcaga agcggatgta atgaaaaaat ggttgataga gaatggcgtt
     2221 gatgaaagca gactgttgct tgatgatgtg gcacgagata ctccgggaaa tgctgtcggt
     2281 atcattgatt ttatgaagga atatggaggt agaaaagtat tggggatcgg tacgatctta
     2341 catttaccga gagcaatgtc tgtattgaaa agctatgcgg attccattgg atatgaattg
     2401 gaaattgatt ctgccggagg cggttctcct cctaatgaaa aaaataaaaa aggtgaagct
     2461 ctttatacat ttgttaatgt tgctcgtgca atggggttat ttaccttggg cgattttgag
     2521 aatttgcttg ccaaagaaca agctgaaaaa gaacaaagag aagcgttgga aaaagcacag
     2581 cagaattatt tagaaaaatt ggtagctgag cgagatcaag cagtaaaaaa tgcagaaaat
     2641 tctgcaaatg agctggaagt agccagacag gcgttagaaa cagcaaaaca ggctcatcag
     2701 caagctgaag aggaattgaa taaagaactt agtaaaacgc aagagcagtt aaaacagagt
     2761 acggaagccc ttagtcgttt aataataaag cagaacaatc ctttttccga gcattatgtt
     2821 gtacagctta atttgatgga acaagcaact tatgatctgt accgtaggtt agcagaaata
     2881 gaacatacac aagattggaa tttatggctt tcggatagtc tatccaaata tcgtttaaaa
     2941 aatcgttcaa ccgaagacta tcacttatta catatcgggc tagatcatca ggtttcccat
     3001 aattggtcta ttggtggagt atttggtatc agtcaggggc aaattgagaa taggacgcat
     3061 aaagtgaaag ggacatctgc tggtatttat gctggttatc gtcctacaag tggtctgttt
     3121 tggacaaatt tttttaatta cagtcatttt actaatcaat ctggtgtaac aaaacgtgat
     3181 aataatgcat acagtctgag tagtgaaatt ggaagaaata tcgtgatgag tcatcaatgg
     3241 caaattacgc caaagctgca attaggctgg gcaaaagctg gatcagcacg aatgttttgg
     3301 gctgggacag ggctacgttt aggaaaagtg attgaactca atgcaatcaa acttaaacct
     3361 tatgctgata ttagctatcg ttttcgaaat cgtcatattg ataccttaga tagtttatct
     3421 gatcgtcatc ggaatgtaat cagtattggg gtacaagcag ttggaaatcg ccattatttt
     3481 gcaatcgaag gatcaatgtt acacggggat gcattgaagc gttcatacgg attacacgca
     3541 aattatcact atcaatggta aataggagcc tctatgaaaa aaatcaaaac aaccctttct
     3601 gcccttgctt taagcctttc tttgataagt gtaaatagct gggcgatacc agctgattat
     3661 ttgagccaag acaatatcag tcaatatgat aaggcgttta actttgtatt agcaaaagta
     3721 ctgcctgcac caaaaggtaa agaagatggt cgaggagcgc cgattgagca tttagttatt
     3781 caatatgcgc cagactatgt tgatgaccct tatctaaaag aggatgtgct gaatttccgc
     3841 cgttggtatt ggcaagcgat gtatgcttcg cttgatcaga aagttaaaga gaagccgacc
     3901 taccttaaca cttaccgttt acaagcagag gcgtatttcg tcaataaaga gtatcgggaa
     3961 gcactttctc agctcgacca aattttgcgc caaaaccccc gtgatgttca cgctcttgcg
     4021 atgacagtat tagcttcaag ggtgctagaa gatactgaac aagaaacggc tcgtctttct
     4081 gcactgaaac agcttgctcc cgaagtcgca caacgaatga cagacttttt tgcctttacc
     4141 gaacaaacgc tggcggcaag ctacggtaat gaaccacaga cagcaatgat ccctgatacg
     4201 attgccgttt ttggttctac gccaaacaaa gacggcacac cttcgccgct gttgttagat
     4261 cgattacaga aaaccaaaga gatggcacaa aaatttccgc aagcaaaaat tatcgtcagt
     4321 ggcggtgctg tgaaaacgga atttgctgag gctgatgtga tggcaaaatg gcttagcgaa
     4381 aatggtattg aaaaagaccg tttgctactt gatcctgttg ccagagatac cccgggaaat
     4441 gcggttggga ttatcaagct gatgcaaacc tataatggca agaaagtatt aggtatcggt
     4501 acgttacaac atatccctcg tgcaacagcg gttctcaaag gttatgcaca gcacattggt
     4561 tatccgattg agatggattc cgttggtggt ggcaagccgg caacaccggg aaaagccaaa
     4621 atcgaggcac tttatactta tgtaaatgtg gcgagatcaa tggggttgtt tgagttgggg
     4681 gattttaagt agttacttta ttgacagctg ctattgataa aaatcccgcg ttaagcggga
     4741 tttttttatg ttctacgaac gtttcataat ctcgaaaaac tcttcgttag tttttgccat
     4801 cattaatttg tcgattagcc attcgatagc atctacttcg tccattgggt tgaggacttt
     4861 gcgtaacatc caagtttttt ggagttcgtc tgctgccatt aagaggtcat ctttacgagt
     4921 acctgagcgg ttgaagtcaa tagccgggaa gatacggcgt tcagcaatac ggcgtgagag
     4981 gtgtaattcc atattacccg tacctttgaa ctcctcgaaa atcacttcgt ccatttttga
     5041 tccggtatcc acaagtgcgg ttgcgataat ggttaagctg ccaccttctt ccacattacg
     5101 ggctgcaccg aagaaacgtt ttggacgatg taaggcgtta gcgtccacac caccggaaag
     5161 gattttgccg gaaaccgggg taatggtgtt gtaggcacgg gctaaacgag tgatggagtc
     5221 taataaaatc accacatctt ttttatgctc taccgagcgt tttgctttct cgattaccat
     5281 ctccgcaact tgaacgtggc gggttgccgg ttcatcaaag gttgaggcaa ttacttcacc
     5341 acgtacggtg cgttgcatct ctgttacttc ttccggacgc tcatcaatta ataatacgat
     5401 aagctcacat tcaggatgat tatgggtgat gctttgtgca atattttgca gtaacacggt
     5461 tttaccagct tttggcgggg caacaattaa cgctcgctga cctttaccga ttggggcggc
     5521 taaatctaaa atacgagcgg ttaaatcttc ttttgatcca ttgccacgct ccatttttaa
     5581 gcgagaattg gcgtgcagag gggttaagtt ttcaaagaga attttactgc gagaggcttc
     5641 cggtttgtca tcattaactg aatcaacttt cagtagggca aaatagcgtt ctccctcttt
     5701 tggtgggcga attttacctt ctattttatc gccagtttgt aagttaaaac ggcggatttg
     5761 gcttggagaa acataaatat catcagggcc ggctaaatat gagctatctg atgagcggag
     5821 gaaaccgaag ccgtccggta gaatttcaag cacgccttgc ccgaaaatgt cttcaccgct
     5881 ttttgcgtgt tgtttaagaa tagcaaatac gatgtcttgt ttacgtaaac gagccagatt
     5941 ttctaagccc atttgatttt cacccatttc cacaagctct gaaacagggg tgtttttaag
     6001 ttgagtaaga tgcataattt gaatgtaaaa ttgtgattaa ttaagattgg atttgttgat
     6061 attgattaga tacggccaac cgtaaagatg tggcgaagaa taacgcaata ggagacaaat
     6121 tgcaaatttt ttataaaaaa taaccgcttg tttgcaataa aaatgccgac atcagccggc
     6181 atttttatat taatttaata ttacgcttca atcccttcaa acagtagcat acttaaataa
     6241 cgctctgagg ctgaaggcag aatcactaca attaatttat cggcaaattc aggtagttta
     6301 gcgatgcgat ctgcggcagc aacggcagca cctgatgaaa taccgcctaa aatgccttct
     6361 tctgccatta aacggcgtgc ggttgtaatc gcttcttcgc ttgaaacttg ttcaacacgg
     6421 tcaagcagtg ataaatctaa gtttttcgga ataaaacctg caccaatgcc ttgaattttg
     6481 tgcgggcccg gtttgacttc ttcaccgttt aaagtttgcg taatgaccgg cgattcactc
     6541 ggttctaccg ccactgaaat aatttgcttg cctttatctt gcttaatagc acgggaaaca
     6601 ccggtaatag tgccacctgt tcctacgcct gccactaata catcaatttt accttcagtt
     6661 gcatcccaaa tttcaggacc ggtagtttgt tggtgaattg ccgggtttgc cgggttttca
     6721 aactgtttta aggtaacata gcgctctggg tcggaagacg cgatttcttc agctttcgca
     6781 atcgctcctt tcattccttt tgccccttcg gttaagacta aatttactcc taagccacgc
     6841 aataagcgtt tacgctcggt actcattgtt tccggcattg tgagcgttaa tttataacca
     6901 cgagcggctg ctacataagc caaggcaatt ccagtgttac cgctggttgc atctacaatt
     6961 tctttgtctt tggttagaat accgtctttc tctgcttgcc acaccatatt cgcaccaata
     7021 cggcatttca cgctaaaact tgggttacgc ccttccactt ttaccacaat attgccgttg
     7081 ccaaaattct ttaaacgcac cagcggggta ttgccgattg agtatgagtt gtctgcatag
     7141 atagtcatag tgatattcct taataagtat tgagattggg atttatcata cttattttta
     7201 atcgcttttc tagtagctgg aatttatttg atataactaa aaagaatata gggtcgtatg
     7261 aagaggatcg gtctgatttt ctttttgcaa aaactcgata aaatcagacc gcttgtgagg
     7321 gttagacctg ggctttattg atcaaaaatt gtgttagtaa ggataccggt ctgccggtcg
     7381 cccctttggc tgccccagat ttccaagccg tgccggcaat atctagatgc gcccaagtat
     7441 atttctttgt aaagttagag aggaattgac cggcagtaca ggctccgcct aaacgtccgc
     7501 cgatattagc aagatcagca aagttagatt ttaactgttc ttgatattct tcccctaaag
     7561 gtaaacgcca cgctttgtca tcggcttgct ctgcggcagc taagagttcg tttgctaaac
     7621 cgttatgagg tgacatcagc ccgctgttat gattacctaa ggcaatcatg caagcaccag
     7681 ttagggtagc aatatcaatc actaattccg gttcaaaacg ttctacatag ctgagagcat
     7741 cacatagcac taaacgtcct tctgcatcgg tgttgagtac ctcaacggtt aaaccagaca
     7801 ttgtagtgag aatatccccc ggtcgatagg ctttgccatc aaccgcattt tcacagccgg
     7861 cgagtaagcc gataacattg agtggtaatt tcatctcggc aattgctttg attgtaccgt
     7921 aaacagtagc agctccgcac atatcgtatt tcatttcgtc catcgcctcc gcaggtttaa
     7981 tggataaacc gccagagtca aaggtcattc ctttgccgac taagataatc ggcttcgcat
     8041 cagcattcgg gttgccttta tattcaatca ctgaaagaaa ggcctcattt tcagaggctc
     8101 tggaaaccgc taagtaagca ttcattccca attctgccat ctgtttttca tcaataatgg
     8161 tagttgaaat ggcaggatag tcgtaggcta aacccttagc caattcggct aaatagtgag
     8221 gattacacac attaggcgga caatttgcta catttttagc gtggctgatt ccaagtgcta
     8281 cggcttttcc gtgttctaaa gctcgttcgg catcaggaag atctttacgg tgtgcgacat
     8341 taaaaatcac acggcgtaat tcacggcgaa cttcaggttt gatgcttttg aaatcgttat
     8401 aggcatatag ttgttcttgg attgcctcta ccgcaaatcg cacattccaa taaggtgttc
     8461 tacctttaac gtgcagttcg gttaaaaagc tgacagcttc cattgagccg gtttcattaa
     8521 tagtttgtac acttttttga ataatttgtt tgtattggcg ttcagttaat tcacgctctt
     8581 ttccacagcc gactaataat actcgatcag cgagtacatt cggcacatta tgcaagagta
     8641 aggtttgccc tgctttgcct tctaaatctc ctcttctcag taatgtgctg atatagccgt
     8701 tacttaattt atcaagctgt tctgcggcag aacataatcg gcggggttca tagacaccaa
     8761 caaccaaaca ggcggttctt tgtttctcaa cactgccatt tttgacacta aattccattt
     8821 ttcctcctta atttttattg ctacagattt tataatgcca acataatcat caatgataag
     8881 taaaatatag atataccctg atgtaaatca ttatttaaaa taatttactt tttatttgca
     8941 aatattaaat gttttttgcg gtcgtataga taattttact ggtttttctt ttagtttgct
     9001 tactttatac taaaagagtt ttttagattt aagagaataa ttggagataa a
//
LOCUS       DANJWC010000096         9027 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.096, whole genome shotgun sequence.
ACCESSION   DANJWC010000096 DANJWC010000000
VERSION     DANJWC010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9027
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.096"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..359
                     /locus_tag="PWX02_001597"
     CDS             <1..359
                     /locus_tag="PWX02_001597"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249596.1"
                     /note="oxidizes malate to oxaloacetate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDL1729593.1"
                     /translation="EQIEQLTHRIQNAGTEVVEAKAGGGSATLSMAQAGAEFALGLVR
                     ALIGEDVIRYAYVDNANGETSPAFFAYPIRLGTNGVEKVLPIGNLSEFEKDQLEQLIP
                     VLNDEIQLGQTFNKNA"
     gene            436..732
                     /locus_tag="PWX02_001598"
     CDS             436..732
                     /locus_tag="PWX02_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729594.1"
                     /translation="MKKHNIIILTLASASVVACRSNTPVQVVNGDKNIDYSQPKTIYI
                     SETNTQPPALTKQEKIKPSNATALCFNGSYSTDIQNPCKKDGGVKQYFAHHHAN"
     gene            complement(781..957)
                     /locus_tag="PWX02_001599"
     CDS             complement(781..957)
                     /locus_tag="PWX02_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5363 domain-containing protein"
                     /protein_id="HDL1729595.1"
                     /translation="MMSEQTEKKGWFKKALEKYDAFCDELGVNQGACRGCVPVVKFDP
                     EPEKEQPEKSENKT"
     gene            complement(1042..1533)
                     /locus_tag="PWX02_001600"
     CDS             complement(1042..1533)
                     /locus_tag="PWX02_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HDL1729596.1"
                     /translation="MPSFDIVSEITMHEVRNAVENANRVLSTRYDFRGVEAVIELNEK
                     NESIKLTTESDFQLEQLIEILIGSCVKRGIEHSSLDIPTESEHHGKLYSKEIKLKQGI
                     ESDMAKKITKLIKDSKIKVQAQIQGEQVRVTGKSRDDLQAAIQLVKGADLGQPFQFNN
                     FRD"
     gene            complement(1549..2412)
                     /gene="serB"
                     /locus_tag="PWX02_001601"
     CDS             complement(1549..2412)
                     /gene="serB"
                     /locus_tag="PWX02_001601"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HDL1729597.1"
                     /translation="MTNTIFIYSKTLNEREIEQFSQQTAAILLKQAVYLDYKIAFFST
                     NLTACELRETAQKINADISDLTIIPTLNQGGLLLMDMDSTAIKIECIDEIAKLAGTGE
                     VVSAITASAMRGELDFEQSLRKRVGTLENASESILQKVRENLPLMDGFEQMVAILKEH
                     GWKLAIASGGFDYFADYLKQKYGLDFAVSNQLEIIDGKLTGKVLGKVVDAQHKADTLK
                     NLATTFNIPQNQWVAVGDGANDLPMLKTASLGVALHAKPKVQAQADFVVNFGDLTALC
                     LLLNANHLFQH"
     gene            2498..3157
                     /locus_tag="PWX02_001602"
     CDS             2498..3157
                     /locus_tag="PWX02_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HDL1729598.1"
                     /translation="MYISREKITKTLLLAGITLLCCGIVAVILMGVNQFKLGSQLASI
                     NQVSNLSHLLVRQQANIFSFLLVKNAKTDELVDALDSFAKEDFIIDANIYSPNGVLLA
                     QSQNAKDLKVLLDSEQSAKTQQIVEPIFSQQALVGFLRVTFDAEYGQTTKARVEQQFH
                     QLYGEIIILLLTGILLASSINYYFRRKIHHVQISPKPLMSQVKTQTQRFHSRRRWIKR
                     K"
     gene            3267..3485
                     /gene="infA"
                     /locus_tag="PWX02_001603"
     CDS             3267..3485
                     /gene="infA"
                     /locus_tag="PWX02_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDL1729599.1"
                     /translation="MAKEDCIEMQGTILETLPNTMFRVELENGHIVTAHISGKMRKNY
                     IRILTGDKVTVEMTPYDLSKARIIFRAR"
     gene            complement(3572..4765)
                     /locus_tag="PWX02_001604"
     CDS             complement(3572..4765)
                     /locus_tag="PWX02_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL1729600.1"
                     /translation="MQNIDVVVIGAGAAGLFCAAQLGQAGKKVVVLDSGKKVGRKILM
                     SGGGFCNFTNLDVSAGNYLSQNRHFVKSALSRYTQWDFISLVSHYGISYHEKELGQLF
                     CDESSQQIVDLLQAECNKGKVEILLRQAVISVEKVANFFEISTACGKFQSEHLVITTG
                     GLSMPALGASPFGYQLAEQFGIPVVPPRASLVPFTWKESDKYFAELSGIALPITVSAN
                     GKSFSNQMLFTHRGLSGPAILQISNYWELGETVEIDLLPSQSITDILSELRQSSPKLQ
                     LKTVLSRYLPKKLVEVWLEQKLLTDKVIADLSKKDLTLLEQFIHHWHFLPNGTEGYRT
                     AEVTKGGVDTDYISSKTMEAKNTAGLYFIGEVLDVTGWLGGYNFQWAWSSAFACAEGI
                     IEKSK"
     gene            complement(4765..5133)
                     /locus_tag="PWX02_001605"
     CDS             complement(4765..5133)
                     /locus_tag="PWX02_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/TusD sulfur relay family protein"
                     /protein_id="HDL1729601.1"
                     /translation="MSNSVQKLLFIINSAPYGNETFFSALRLALQIQEQHKANTEIKL
                     FLMSDAVTAGLAKQNPAEGYHIQQMLEILTAQGANVKLCKTCTNARGITDLPLADGVE
                     IGTLIELADWTIQADKVLNF"
     gene            complement(5252..6547)
                     /locus_tag="PWX02_001606"
     CDS             complement(5252..6547)
                     /locus_tag="PWX02_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL1729602.1"
                     /translation="MLEKLFQLKAKGSNVKTEIIAGITTFFTMVYIVFVNPSILGVAG
                     MDTQVVFVTTCLIAAFGTIAMGLFSNLPIALAPAMGLNAFFAFVVVQKLGYSWQVGIG
                     AIFLGSVGLFLLTVLQVRYWFMSAIPLGLRVGIGAGIGLFIALIGFKNMGLVVPNPAT
                     LVALGDLHDPKVLMGILGFFIIVVLAAKGIHSGVLISIVIVTALALAFDPAVSFNGIM
                     SMPPSLGAVVGQVDIAGAFDVALLGIIFSFMLVNLFDSSGTLIAVTTKAGFADEQGRF
                     PKMKQALLVDSTAAMAGSYMGTSAISTYIESGSGVSVGGRTGLTAVLVGILFLLTIFF
                     SPLAGLVPAYATAGALVFVGILMASSLIEVKWDDLTEATPAFITTAMMPFTYSITEGI
                     AFGFISYCVMKAFTGRWKEINLSVLVVSLLFIAKFVWVG"
     gene            complement(6644..7630)
                     /locus_tag="PWX02_001607"
     CDS             complement(6644..7630)
                     /locus_tag="PWX02_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL1729603.1"
                     /translation="MSNIHLAIAADFTLAEKLLEALANQDLGIENISAVELEPFGEEQ
                     NLRFGAKAVAQYALDEVNWAEFSHVLFAGKMAQAEVLAEAVKAGCVILDLYGITALIG
                     DVPVVVPSVNDENVANLRERNIVALANPQISQLALALKPLLNEPLKQVWVTSLLPSAY
                     FGDEKVKELAGQTARLLNGIPFDENAERIAFDVVPANVQGEEKSLPFSKAFELQIAKV
                     LPNLTACTTFHSVQVPVFYGVSQMVSVQSEYQLDANVLSEAWKAENWLNFNENSVITA
                     VKNGENEEQNALEISQLLAKSEQEVQFWTVADEQRFSLAFLAAKLLKLVLAY"
     gene            7871..8146
                     /gene="ybfE"
                     /locus_tag="PWX02_001608"
     CDS             7871..8146
                     /gene="ybfE"
                     /locus_tag="PWX02_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HDL1729604.1"
                     /translation="MAKQDSDCITLDLFATVPKVGRPRTNPLDREQQLRINKRNQLKR
                     DKSSGLKRVELKLHSDLVKLLEEQASEQGVSRGQLIETILNNYLTNR"
     gene            8152..8676
                     /gene="fldA"
                     /locus_tag="PWX02_001609"
     CDS             8152..8676
                     /gene="fldA"
                     /locus_tag="PWX02_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HDL1729605.1"
                     /translation="MAVVGLFYGSDTGNTENISKMIQKKLGAELIDIRDIAKSTKEDI
                     EAYDFLMFGIPTWYYGESQADWDDFMPTLKEIDFNGKVVAIFGCGDQEDYAEYFCDAM
                     GTVRDVVEPNGGVIVGHWPTAGYTFEVSQALVDDDTFVGLCIDEDRQPELTTERVNKW
                     CEQLKEEMCLDQLA"
     gene            8712..>9027
                     /gene="fur"
                     /locus_tag="PWX02_001610"
     CDS             8712..>9027
                     /gene="fur"
                     /locus_tag="PWX02_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HDL1729606.1"
                     /translation="MSEANIKRLKEVGLKVTEPRLTILALMQDIRDEMQHFAAEDIYK
                     ILLEKGSDIGLATVYRVLNQFEEAGILTRHNFDANKAVFELNMNHEHDHIICMDCGKV
                     FEF"
BASE COUNT         2754 a         1918 c         1715 g         2640 t
ORIGIN      
        1 aagaacaaat cgagcaatta actcatcgta tccaaaacgc cggtactgaa gtggttgagg
       61 caaaagcagg tggtggctca gcaaccttat ctatggctca agcaggagca gaatttgcac
      121 ttggtttagt gagagcctta atcggggaag atgtcattcg ttatgcttat gtagataatg
      181 ccaatggcga aacctctccg gcattctttg cttacccgat tcgtttaggt acaaatggtg
      241 tggaaaaagt tctacctatc ggcaatctca gcgaatttga aaaagatcaa ctagagcagt
      301 taattcctgt attaaatgat gaaattcagt taggacaaac ttttaataaa aatgcttaag
      361 gcataacttt aagggttgag aaatcagccc tttttaacat tctctcaatt aaaaatataa
      421 aaacggaaat tacccatgaa aaaacacaat atcattatac ttacattggc ttcagcatcg
      481 gttgtagctt gcagatcgaa cacccccgtt caagtcgtaa atggcgacaa aaatatagac
      541 tattctcaac ctaaaacgat ctatattagt gaaaccaata ctcaaccgcc agccctaact
      601 aaacaggaaa agattaaacc atcgaatgct actgctcttt gttttaacgg tagttactct
      661 actgatattc agaatccctg caagaaagat ggcggagtta agcaatattt tgcccatcat
      721 cacgccaatt aaaaacccca taaaaaaatg cacaatgtat cttcccattg tgcatttttt
      781 ctatgtttta ttttctgatt tttctggctg ctctttttcc ggttcagggt caaacttcac
      841 cacagggaca caacctcggc aagcaccttg atttacccct agctcatcgc aaaaggcatc
      901 atatttctca agtgcttttt taaaccagcc tttcttttct gtttgctcgc tcatcatctc
      961 ttccttttat gttgaattat taatgcaagc ggtcaaattt tgctgaattt ttccacttgt
     1021 catcattaat tgcaggaatt gctaatcacg gaaattatta aattggaatg gctgccctaa
     1081 atctgcccct ttcactagct gaattgcggc ttgtaaatca tctctggatt taccggtcac
     1141 acgcacttgt tcaccctgaa tttgggcttg aaccttgatt ttagaatcct taatcagctt
     1201 ggtaattttc ttcgccatat ctgactcaat cccttgcttg agcttaattt ctttggaata
     1261 gagtttaccg tgatgctcac tttctgtagg gatatctaat gaactatgct cgatcccacg
     1321 ttttacgcaa gagccgatta aaatctcaat taactgctct aattggaaat ccgattcggt
     1381 cgtgagctta atgctttcat ttttttcatt taactcaata acggcttcca ccccacggaa
     1441 atcgtagcgg gtgcttaata cacgattggc attttctacc gcattacgca cttcgtgcat
     1501 cgtgatttca gatacaatat caaatgatgg cattgttgtt cctctcaatt aatgttgaaa
     1561 taaatgattg gcattaagta ataaacataa tgccgttaag tccccgaagt ttaccacaaa
     1621 atcagcttgt gcttgcactt ttggtttagc gtgcaaagca acgcctaaac tggcggtttt
     1681 taacattggc agatcgtttg ccccatcgcc tactgcaacc cattgatttt gtggaatatt
     1741 aaaggttgtt gccaaatttt ttaaggtatc ggctttatgt tgagcatcca ctactttgcc
     1801 taacaccttg ccggttaatt taccgtcaat aatttccagt tggttagaga ccgcaaaatc
     1861 caacccgtat ttttgtttta agtaatcggc gaagtaatca aatccaccgg acgcaatcgc
     1921 taatttccag ccgtgttcct taagaatagc aaccatctgc tcaaagccgt ccataagcgg
     1981 tagattttcc cgcacttttt gcaaaatgct ttccgatgca ttttccagcg tgccgactcg
     2041 cttgcgtaaa ctttgctcga aatccagctc gccccgcatt gcactggcag taatggcgga
     2101 caccacctcg ccggtgccgg caagtttggc aatctcatca atacattcaa ttttaatagc
     2161 ggtggaatcc atatccatta atagcaaacc gccttggttt aaggttggaa taatagtgag
     2221 atcgctaata tccgcattga ttttctgtgc cgtttcacgc agctcgcaag cggttaaatt
     2281 tgtggaaaaa aatgcaattt tgtagtctaa atagaccgct tgtttcagca aaattgctgc
     2341 cgtttgttgt gaaaactgtt caatttcacg ctcgtttaag gtttttgaat agataaaaat
     2401 tgtgtttgtc attgtcttat tttaggttgt ttaaatttac tgcactaatc taacagaaaa
     2461 gcgtgctaca atcacgcatc attttgaggg aatttcaatg tacattagtc gtgaaaaaat
     2521 aacgaaaacg cttttgctgg cagggattac gctactctgc tgtgggatcg tggctgttat
     2581 tttaatgggc gtaaaccaat ttaagctagg ctcacaatta gccagcatta atcaagtatc
     2641 aaacctttca cacctattgg ttcgccagca agcgaatatt ttttcgtttt tgttagttaa
     2701 aaatgccaaa accgatgagc tggttgatgc gttggatagt tttgccaaag aagattttat
     2761 tattgatgcc aatatctatt caccaaatgg cgtgctactg gctcaaagcc aaaatgcgaa
     2821 agatttaaaa gtattgctcg attcagagca atctgccaaa actcagcaaa ttgttgagcc
     2881 aatattttct caacaggcgt tggttggctt tttacgcgtc acgtttgatg cagaatatgg
     2941 gcaaactact aaagcacgag tagagcaaca atttcatcaa ctttatgggg aaataattat
     3001 tttgctactc acaggtatat tgttggcaag tagcattaac tactattttc gtcgtaaaat
     3061 tcatcatgta cagataagcc caaaaccact tatgagtcaa gtaaaaaccc aaacccagcg
     3121 ttttcactca cgtcgccgtt ggattaagcg taaatagttt acactcaaat ggataaacga
     3181 ttgtaatttg agcgaataag cgtataatat ccgccgtttt tcgccgaatg gcatcatata
     3241 aaaattaaaa ctttaaagag gaataaatgg ctaaagaaga ttgcattgag atgcaaggta
     3301 cgattttaga aaccttacca aatacaatgt tccgtgtgga attagaaaac ggtcatattg
     3361 ttaccgcaca tatttcaggc aaaatgcgta aaaactacat tcgtatttta accggtgata
     3421 aagtaacggt tgaaatgact ccatacgatt taagcaaagc acgcattatt ttccgtgcaa
     3481 gataattcag cataaaataa ggcgaacaat tgtgttcgcc tttttgtttg gttttttagt
     3541 tatttgcttt tttagttatt tgctttttta gttatttgct tttttcaata atcccttctg
     3601 cacaggcaaa agcagatgac cacgcccatt ggaaattata accaccaagc cagccggtaa
     3661 catccagcac ttcgccgata aaataaagcc ctgctgtatt tttcgcttcc atcgttttag
     3721 atgaaatata gtcggtatca acaccgcctt tggttacttc cgctgtacga tagccttccg
     3781 tgccgtttgg caagaaatgc caatggtgga taaattgctc taataaggtc agatcttttt
     3841 tactcaaatc ggcaatcact ttatcggtca gcaatttctg ttccagccaa acttcaacca
     3901 attttttagg taaataacga gataatacgg tttttagctg taatttcggc gaagattggc
     3961 gtagttcgct caatatatcg gtaatggact gcgaaggtag cagatcaatc tctaccgttt
     4021 cccccaattc ccaataattg gaaatctgca aaattgccgg gccggataag cctctgtgcg
     4081 taaacaacat ctgattgcta aaacttttgc cattggcgga aacagtaatc ggcagtgcaa
     4141 tgccggaaag ctcggcaaaa tatttatcgc tctctttcca agtaaaaggc acaaggcttg
     4201 cacgtggtgg cacaacagga ataccaaact gctcggcaag ttgatagcca aaagggcttg
     4261 ccccgagagc cggcattgaa agccctccgg ttgtaatcac taagtgttcg gattggaact
     4321 tgccacaagc ggtcgaaatt tcaaaaaaat ttgcaacttt ttccacagaa atgaccgctt
     4381 gccgtaacag aatctcaacc ttgcctttgt tgcattctgc ttgtagtaaa tcgacaattt
     4441 gttgcgagct ttcatcacaa aacaactgcc ctagctcttt ttcgtgatag ctaatgccat
     4501 agtggctgac aagcgaaata aaatcccatt gggtataacg tgaaagggcg gatttcacaa
     4561 aatggcggtt ttggctaagg taatttccgg cacttacgtc taaattcgta aaattgcaaa
     4621 aaccaccgcc agacattaaa attttacgtc cgactttctt accgctgtct aacaccacca
     4681 cttttttgcc agcttgcccc agttgggcag cacaaaacag ccctgccgca ccggcaccaa
     4741 tcaccacaac atcaatattt tgcattaaaa attcagtacc ttatctgctt gaattgtcca
     4801 atctgccagt tcaattaatg tgccaatctc taccccatcg gctaaaggca aatctgtaat
     4861 ccctcgagca ttagtacagg ttttgcataa ttttacgttt gccccttggg cggttaaaat
     4921 ttccagcatt tgctgaatat gataaccctc tgccggattt tgctttgcca gccctgcggt
     4981 gactgcatcc gacattaaga aaagtttaat ttccgtattg gctttatgtt gttcttgaat
     5041 ttgtaatgcc aaacgcaacg cactgaaaaa cgtttcattg ccgtaaggag cagaattgat
     5101 gataaaaaga agtttttgaa ctgaattaga cattgtttta cctcggaata cacgaaacac
     5161 atagataatg aatcaaatac cctctcccct ttgtgggaga gggatagatt tttcttcgtt
     5221 tagaagaaaa atcagggaga gggggtagag cttaacccac ccacacaaat ttagcaataa
     5281 agagaagtga aaccactaaa acagaaaggt tgatttcttt ccaacgccct gtaaaggctt
     5341 tcattacgca gtagctgata aagccgaatg caatcccttc agtgatggag taagtaaacg
     5401 gcatcattgc ggtagtaata aatgccggcg tagcttcagt taaatcgtcc cattttactt
     5461 caattaagct agatgccatt aaaattccta caaataccaa tgcccccgca gttgcatagg
     5521 caggtactaa ccctgcaagt ggtgagaaga aaatggttaa taggaataag atcccaacta
     5581 acaccgcagt taagccggta cgaccgccaa ctgaaacgcc ggagccactt tcaatatagg
     5641 tagaaatcgc tgaagttccc atataagagc ctgccattgc agcggtacta tctactagca
     5701 gagcttgttt cattttcggg aaacgccctt gttcatccgc aaagcctgct ttggtagtca
     5761 ccgcaattaa ggtgccggaa gagtcaaaca ggtttaccaa cataaaggag aaaatgatcc
     5821 cgagtaaggc aacatcaaaa gcaccggcaa tatcaacctg ccccacaact gccccaaggc
     5881 ttggtggcat agacattatg ccgttaaagc tcaccgccgg gtcaaaggct aaggcaagag
     5941 cggtaacaat cacaattgaa attaacacgc ctgagtgaat acctttcgcc gctaatacaa
     6001 caatgatgaa aaagcctaaa atccccatta acactttcgg gtcgtgcaaa tcacctagtg
     6061 ccacaagcgt tgccggattt ggcaccacca agcccatatt tttaaaaccg attagggcaa
     6121 tgaataaacc gataccggca ccaataccta cacgtaaacc aagtggaatc gcagacataa
     6181 accagtaacg aacttgtaat acagttaata ggaataagcc aacagagcct aagaaaatcg
     6241 cccctatacc cacttgccac gagtaaccta atttttgcac cactacgaat gcaaaaaagg
     6301 catttaagcc cattgccggt gcaagagcaa tcggtaaatt actgaataac cccattgcga
     6361 tagtaccaaa agcggcaatt aaacaagtag tcacgaacac tacttgggtg tccattcccg
     6421 ccacgcctaa aatagacgga ttgacgaaaa caatataaac cattgtgaaa aaggtagtaa
     6481 taccggcgat aatttcggtt tttacattac tgccttttgc tttgagctga aataattttt
     6541 ctaacataaa accttcggtg tttaaacaaa atgacaacgc ccaagcaatc ggttgctcgg
     6601 gcgttgacga tttttatcac ggaataaata cttttgcaag cacttaatac gctaaaacta
     6661 attttagtaa tttcgccgct aaaaatgcca gactgaaacg ctgttcatcg gcgaccgtcc
     6721 agaactgcac ttcctgttcg gatttcgcca ataactgact gatttctaac gcattttgct
     6781 cttcgttttc gccatttttc accgctgtaa tcactgaatt ttcattaaaa ttcagccaat
     6841 tttcggcttt ccaagcttca cttaacacat tagcatctaa ctgatattca gactgcaccg
     6901 acaccatttg ggaaacccca taaaagaccg gcacttgcac gctgtggaag gtagtacaag
     6961 cggtcaaatt tggtaaaact tttgcaattt gcagttcaaa ggcttttgag aacggcagac
     7021 ttttctcttc tccttgcaca ttagccggca caacatcaaa ggcaatgcgt tccgcatttt
     7081 catcaaacgg aatgccgttt aacaaacggg cagtttgtcc ggcaagctcc ttgacttttt
     7141 catcgccaaa ataggcagac ggtaaaagtg aggttaccca cacctgtttt aacggctcgt
     7201 tcaacaatgg ctttaaggct aacgccagtt gtgaaatttg cggattggct aaagcaacga
     7261 tatttcgctc acgcaaattt gcgacatttt catcatttac acttggcaca actaccggta
     7321 catcgccgat gagtgcggta atgccgtata aatctaaaat cacacagccg gctttcaccg
     7381 cttctgccaa cacttccgct tgtgccattt tgccggcaaa tagaacatgg ctaaattccg
     7441 cccaatttac ttcatctaac gcatattgtg cgactgcttt tgccccaaag cgtaaattct
     7501 gctcttcacc aaacggctca agctcaacgg ctgaaatatt ttcaatccct aaatcttgat
     7561 ttgctaatgc ttcaagtaat ttttctgcca gcgtaaaatc agcggcaatc gctaaatgga
     7621 tattcgacac actatctcct ttttcataac gttattcggt ttcattataa gctaatttca
     7681 cgcttcagaa aaatcataac tcataatgtg taatttatga tttttgcaaa ttttaaacct
     7741 aaaaacaccg cttgttttgc ctgtaattta cttttcaaat gattgaactt ttgatatagt
     7801 tcacacttta aaagtagctt atcattctca ttaaaactga ggattagcta aagcccaata
     7861 gtaaaacaaa atggcaaaac aagattcaga ttgtataacc ttagatctgt ttgcgactgt
     7921 accaaaagtt gggcgtcctc gtacaaatcc tcttgataga gaacaacaac ttcgaatcaa
     7981 taaacgtaac caactaaaac gagataaatc ttccggttta aaacgggtag aattaaaact
     8041 gcattcggat ttagtaaagt tattagagga acaggcgtct gaacaaggtg tttcacgcgg
     8101 gcagttaatt gaaacaattc taaataatta tctcacaaat aggtagtagc aatggcagtt
     8161 gtaggtttat tttacggtag cgatacaggc aatacagaaa atatttcaaa aatgattcag
     8221 aaaaaattag gggctgaatt aattgatatt cgagacattg ccaaaagcac aaaagaagat
     8281 attgaagcat acgattttct gatgtttggg atcccaactt ggtattatgg tgaatcgcaa
     8341 gccgattggg acgattttat gcctacccta aaagagattg attttaacgg caaagtcgtt
     8401 gcaatttttg gttgcggtga tcaggaagat tatgcagaat atttctgtga tgcaatgggt
     8461 actgtgcgtg atgtggtaga gccaaatggt ggtgtgattg ttggtcattg gccaactgcc
     8521 ggttacactt tcgaggtttc tcaggcctta gttgatgatg atacttttgt cggcttatgt
     8581 attgatgaag atcgtcagcc tgaattaacc actgaacgtg ttaataaatg gtgtgagcag
     8641 ttgaaagaag aaatgtgctt agaccaactc gcataataac tattttggat aaagataaat
     8701 aggtggcata tatgtctgaa gcaaatatta aacgtttaaa agaggtaggc ttaaaagtaa
     8761 ccgagcctcg tttgactatc cttgcattaa tgcaagatat tcgtgatgaa atgcaacatt
     8821 ttgcagcaga agatatttat aagattctgt tagaaaaagg ctcagacatt ggtttagcaa
     8881 cggtttatcg tgtcttaaac caatttgaag aagccggcat tttaacccgc cataatttcg
     8941 atgcgaataa agcagtattc gaattaaata tgaaccacga acacgaccat attatttgta
     9001 tggattgtgg taaggtgttc gaattta
//
LOCUS       DANJWC010000097         8788 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.097, whole genome shotgun sequence.
ACCESSION   DANJWC010000097 DANJWC010000000
VERSION     DANJWC010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8788
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.097"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(40..813)
                     /locus_tag="PWX02_001611"
     CDS             complement(40..813)
                     /locus_tag="PWX02_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDL1729607.1"
                     /translation="MNLSNALKLIKYNELDVLEVNHNKFKAKIALQGAQLLSWQPYNA
                     EQDVLWLSEIEPFRIGSAIRGGIPICYPWFGSIKEPAHGTARIQEWRLVEHTCSDENV
                     GLVFALENKAKMEMMLGETCELYFTHLDPEPAQLALHSYFNIGDIEKVEVQGLPTRCF
                     DKLTDQEVEVPSPRKISENVDCIYPAQAVNFIQDFANQRTIKVEHINATETVLWNPWH
                     NTVSAMTETAYQNMVCVETARINTLLQQNETLGVRVLVR"
     gene            962..5158
                     /locus_tag="PWX02_001612"
     CDS             962..5158
                     /locus_tag="PWX02_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL1729608.1"
                     /translation="MKTKTFTRSYLASFVTIVLSLPAVASVVRNDVDYQYFRDFAENK
                     GPFSVGSMNIDIKDNNGQLVGTMLHNLPMVDFSAMVRGGYSTLIAPQYLVSVAHNTGY
                     KNVQFGAAGYNPDSHHYTYKIVDRNDYEKVQGGLHPDYHTPRLNKLVTEVVPAAVTNA
                     GTSIKPYLNEERFPMFLRAGSGTQALRGKESNKTTGIAGAYEYLTGGTTLQLSKSSPD
                     HWLDYSSNLYQVSYGPLSTYALPGDSGSGSYAYDMNEKRWVLVGVLNFYNGMDNQFNR
                     SAIIRKDFHEKKFAEDIAGTINNTVQNAQFNWTAQGKSSSLSQSNNVQKLNVDLKDSS
                     IANQNTSLPQENHGKTINFNGKDATIVLKQDIDQGAGALNLNANLTIRPETDQTWQGA
                     GIIVGKDKKVNWQVKNPQGDRLSKLGEGTLYVNGRGQNLGDISVGDGIVILNQQADHQ
                     GRKQAFNTVGIVSGRPTVVLGSADQVNPDNIYFGFRGGRLDLNGNSIAFKRIQNSDKH
                     ARIVNHNRDHISTLIIQGQDPLTSNDLIWGKWASNSPADIYEYTNPYQNKRKDYFRLK
                     GNSRVYYPTNATSNDHWEFLSSNREQAIQKILDAKNLRQRYDTFNGFIGEDASNKTNG
                     ILNVVFDTKTEVNTEQDKLKNIYTMSGGFNLNGELTLKGGTLLLSGHPTPHAYDIKNK
                     HDVVRENDWQDSHFTAKNITVNKMAQLYIGRNVNEVNSHFTATDKAKLNLGFINRSTP
                     SCYDSEYTGTTHCEVQAVISDNIFANLATTAIKGNVKLQNHSQLNLGKANLTGSVQAD
                     QTTHITLANHSHWLNNGTSQIGHLTMEKGSILSLNDKFATTEIPVRFNKMIIQGNLKG
                     NGRINYTANLAKGESDHLQVDGIAEGNFVLAVRNSTTEANPKSSLNLLSLKNSNQEGN
                     KVSISLENNYVDLGTYRYVLENRNHNYHLFNPLIPNSTSKEMNATSVSSIPKKESVTN
                     VPTLDKKETEQNLTQLQKDFSAHQLENQKAKQSMINAQSELRRLNSQLNVLQKYVNSR
                     RLGYYTQQAVLEQISIIQNKIKQTQTIFNDANATVKLTDQKLEEAKLALGSVNDLVLI
                     KASAPAMQATNQDTSMMNIIQADWISQYANTALSELSAQANSALQISNSLDRQLFKQS
                     DKFNVWSSVEHQKTEHKSDLYRPYKQQTNLTQLGIQMPIDNGLMFGVALSKNHANAEF
                     NEGVNGKSNLLMASLYGKWQSQQGTFISLDGSYGKAKNQLYLFGENHFTRRISSIGAN
                     IGHQFDLAGVQIQPTIGARYYHFSGQDYTLGGAKISSPNTHFMTYQAGLKASKTFSLD
                     DWKVEPSITTHYVDASNKRLAVNVNNNNINQRFGRYLKTEAGIRFEHNQWQFDLHAGF
                     INGNEIKKQHFAGVKLGYSW"
     gene            complement(5213..6307)
                     /gene="mobA"
                     /locus_tag="PWX02_001613"
     CDS             complement(5213..6307)
                     /gene="mobA"
                     /locus_tag="PWX02_001613"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HDL1729609.1"
                     /translation="MSKTIEERISAVILAGGLARRMNGVEKGLQLFEGKPLISHILAR
                     LSPQVSDIWLNVNRSIEQYQQLYPAFSYYQDSLPDFQGPLSGMLAGFEQIESDYLLFV
                     PCDTPFMPELLLQKLKTALRINNVQIAYAHDGERPHPTFALIHRSVQEDLKAYLGSNQ
                     QQLLAFFQSQKSVAVDFSEQKSAFTNVNTLEDLSRVSQFPVKNLAITGYSGTGKTTLL
                     EKLIPKLTACGIRVGLIKHSHHNVDVDKKGKDSYRLREAGANPTMIVCDERWALMTET
                     KQAVEFSQLIAKFNPQEVDLVFVEGFKHETLPKIQLHRKGIEKPLPELDQWTIATATD
                     YPLERENWLDINNIGGIADFIKNWLENKAS"
     gene            6394..6663
                     /locus_tag="PWX02_001614"
     CDS             6394..6663
                     /locus_tag="PWX02_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1040 family protein"
                     /protein_id="HDL1729610.1"
                     /translation="MKCERLEELLELLGEHWRKNPDLHLIDILQQLSVEVGEPDNFKA
                     LSDEVLIYQLKMRNAGKFEPIPGIKKDYEDDFKTALLRARGILND"
     gene            6727..7365
                     /locus_tag="PWX02_001615"
     CDS             6727..7365
                     /locus_tag="PWX02_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL1729611.1"
                     /translation="MKKLALKTAFVALSALFTFNSTAIAADPTAGKEYIEVRKAPSAQ
                     KEVVEFFSFYCPHCYDFELSYKIPSQIKEKLPSDSKLVQYHVNFLGRQSEDLTRAWAL
                     AMALGAEDKVKTALFEGAQKDAFKSMDDIRSVFLANGITAEQFDSGINSFAVNGLVNK
                     QVQLAEDFQIRGVPAFFVNGQYQLNLEGFADSSSTNDFIKRYVDAVVFLSKK"
     gene            7449..8489
                     /locus_tag="PWX02_001616"
     CDS             7449..8489
                     /locus_tag="PWX02_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDL1729612.1"
                     /translation="MTKLSILNLVPIREGQTAKQAVDSMVKLAKHAESIGIERYWIAE
                     HHNMKNLASSATQLLIQHALSHTEKLRVGSGGVMLPNHSPYIVAEQYGTLETLYPNRV
                     ELGLGRAPGTDMRTATALRRNAQSLPFPDEISELKGYFEGTNPVSAYPAQGLNVPFYI
                     LGSSPESAYLAAERGLPYAFASHFAPRFMDDAVRIYRTHFKPSEVLDKPYVILGVNAI
                     AAETDQEAEQLATTQTQFFLNVVTNAQNYLQPPVESDDKVWENFVKAEIVPHFGPVSF
                     QQNALYNQEKTVVRQMTACSLIGSRESVEQQLKSLKARIEIDEIMAVSYIFDEQKQHQ
                     SYTWLKEIADKV"
     gene            8491..8784
                     /locus_tag="PWX02_001617"
     CDS             8491..8784
                     /locus_tag="PWX02_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDL1729613.1"
                     /translation="MSKTMQQKAADAHNMCKLKGDSMLDNSDRQIAFEAVYDSEEIAV
                     QAVEFFTQKAKSVETEPCDIQSEITPIIDGFLMKMWITFSCQAEVILFQMAVR"
BASE COUNT         2903 a         1757 c         1628 g         2500 t
ORIGIN      
        1 aacaatatta ctaattcagc tgcttgctct cattaaacac tatcgcacta aaaccctcac
       61 tcctagcgtt tcattttgtt gaagtagagt atttatccga gcggtttcca cacataccat
      121 attttgatac gccgtttctg tcatcgcact aacggtatta tgccaaggat tccaaagtac
      181 ggtttcggtc gcattaatat gctcaacttt aatggtgcgc tgatttgcaa aatcttgaat
      241 aaaattcacc gcttgtgccg gataaataca atccacattt tcacttatct tgcgaggtga
      301 tggcacttca acttcttgat cggttagctt atcaaaacag cgagttggca agccttgcac
      361 ttccactttt tcaatatcac caatattaaa ataagagtgc aatgccagtt gtgctggttc
      421 tggatctaaa tgggtaaaat ataactcaca agtttcgcct aacatcattt ccattttcgc
      481 tttattctct aaggcaaata ccaaaccgac attttcatca gaacaagtat gctcaactaa
      541 acgccattct tgaatgcgag ctgtgccgtg ggcaggctct tttattgaac cgaaccacgg
      601 ataacaaatt ggtataccgc cacgaatcgc agagccaatt ctaaacggtt ctatctcact
      661 taaccacagc acatcttgct ctgcattata cggctgccaa cttagcaatt gtgctccttg
      721 taatgcaatt ttagctttga atttattgtg attaacttct agcacgtcta attcattata
      781 tttgattaat tttaatgcat tactcaagtt cataatttat tcctttgatg actaaaataa
      841 ttgacagcaa acactactta gtaattttat ataaaataag ggaagacatc ttgccaaact
      901 atcagttata agacagaata gccagaattt tttattactt ataaggataa acttctcatt
      961 tatgaagacc aaaacattta ctcgttctta tcttgcttct tttgtaacaa tcgtattaag
     1021 tttacctgct gtagcatctg ttgtacgtaa tgatgtggac tatcaatact tccgcgattt
     1081 tgccgaaaat aaaggaccat tttcagttgg ttcaatgaat attgatatta aagacaacaa
     1141 tggacaactt gtaggcacga tgcttcataa tttaccaatg gttgatttta gtgctatggt
     1201 aagaggtgga tattctactt taattgcacc acaatattta gttagtgttg cacataatac
     1261 tggatataaa aatgttcaat ttggtgctgc aggttataac cctgattcac atcactatac
     1321 ttataaaatt gttgaccgca atgattatga aaaggttcaa ggagggttgc acccagacta
     1381 tcatactcct cgattaaata aattagtaac agaagttgtg cctgccgcag tcaccaatgc
     1441 aggtacatct attaaaccct acttaaatga agaacgcttc cctatgtttc ttcgtgctgg
     1501 ttcagggaca caagcgctaa gaggaaaaga aagtaataaa acaactggaa tcgctggtgc
     1561 ttatgaatat cttactggcg gtaccacatt acaattatct aaaagctccc ctgatcactg
     1621 gttagattat tcaagtaacc tttatcaagt aagctatgga ccactttcaa cctatgcact
     1681 acctggtgat agtggttcag gttcttacgc ctatgatatg aacgaaaaac gatgggtatt
     1741 agttggtgtg ctcaatttct ataatggtat ggataatcaa ttcaaccgct ctgcgattat
     1801 ccgtaaagat ttccacgaga aaaaatttgc cgaagatatt gcaggaacaa tcaataatac
     1861 cgtacaaaat gcacaattca attggactgc tcaaggtaaa tccagctctc ttagtcaatc
     1921 aaataatgtg caaaaactca acgttgatct aaaagatagt agcattgcaa accaaaacac
     1981 ttctctgcca caagaaaatc acggtaaaac cattaatttt aatggtaaag atgcaactat
     2041 tgtactaaaa caggatattg accaaggtgc aggtgcatta aatctgaacg ctaatctcac
     2101 tattcgtcct gaaacagacc aaacttggca aggtgcaggt attatcgtcg gtaaagataa
     2161 aaaagtgaat tggcaagtaa aaaatccaca aggcgatcgt ttatctaaac tcggggaagg
     2221 aacactctat gtaaatggac gtggacagaa tcttggcgat atcagtgtag gtgatggtat
     2281 tgtaatactt aaccagcaag ccgatcacca aggaagaaaa caggccttta atacagtagg
     2341 aatcgtaagt ggtcgcccca ctgttgtgct aggtagtgca gatcaagtta atcccgataa
     2401 tatttacttt ggatttcgcg gaggtcgttt agacctaaac ggtaacagca tcgcctttaa
     2461 acgtattcaa aacagcgata aacatgctcg tattgtaaac cacaatcgcg atcacatttc
     2521 taccttaata atacaaggcc aagatcctct cactagtaat gatcttatat ggggaaaatg
     2581 ggcaagtaat agcccagcag acatttacga atataccaat ccttatcaaa ataaacgcaa
     2641 agattacttc cgtctgaaag gtaattcgag agtatattat ccaacgaatg ctacaagtaa
     2701 cgatcactgg gaatttcttt ccagtaaccg cgagcaagca atacagaaaa tcctagatgc
     2761 caaaaactta agacagcgct atgacacgtt taatggtttt ataggggaag atgcttccaa
     2821 taaaactaat gggatattaa atgtcgtgtt tgatacaaaa acagaagtaa atacagaaca
     2881 agataaatta aagaatatct acacaatgtc gggaggattt aaccttaatg gtgaactcac
     2941 ccttaaaggt ggtacattgt tgctttctgg tcacccaacg ccacacgctt atgatattaa
     3001 gaataagcat gatgttgtgc gtgaaaacga ttggcaagac agccatttta ctgctaaaaa
     3061 tatcacggta aataaaatgg cacaactcta tatcgggaga aatgtcaatg aagtaaatag
     3121 tcactttact gcgactgata aagccaaact caatttagga tttattaatc gttcaacgcc
     3181 aagttgctat gattctgaat acacaggcac tacacattgt gaagtgcaag cggtcatttc
     3241 cgataatatt tttgcaaatc tagcaacaac cgccattaaa ggtaatgtta aattacaaaa
     3301 ccatagccaa ttaaatttag gcaaagcaaa cctcactggt tctgtacaag ctgatcaaac
     3361 aactcatatc actttagcaa atcacagtca ctggttaaac aatggtacga gccagattgg
     3421 gcatcttaca atggaaaaag ggtcgatcct tagcctaaac gataaatttg ctaccacgga
     3481 aatcccagtc cgattcaaca agatgatcat ccaaggtaat ctaaaaggta atggacgaat
     3541 taactatacc gcaaatttag ccaagggcga atctgatcat ctccaagttg acggtattgc
     3601 tgaaggaaat tttgtccttg ccgttagaaa tagcacaact gaagcaaatc caaaaagctc
     3661 attaaaccta ctaagcttaa aaaatagcaa ccaagaaggc aataaagttt ctatttctct
     3721 agaaaataat tatgttgatc taggtactta tcgttatgta ttagaaaatc gtaatcacaa
     3781 ttaccattta tttaatccat taataccaaa ttcaacctct aaagagatga atgctacatc
     3841 tgtatcctct attccaaaaa aggaatctgt tactaatgtt cctactttag ataagaaaga
     3901 aactgaacaa aatcttactc aactacaaaa agatttttca gcacaccaat tagaaaatca
     3961 aaaagcaaaa caatctatga taaatgctca atctgagcta agacgactca attcacaact
     4021 gaatgtattg caaaaatatg tgaattctcg tcgcttaggt tactatactc agcaggcagt
     4081 tttagaacaa attagcatta ttcaaaataa aattaaacaa acacaaacaa tatttaatga
     4141 cgctaatgca actgtaaaac tcacagatca aaagctagaa gaagccaaat tagctctagg
     4201 ctctgtaaac gatcttgtat taataaaagc ctctgctcca gcaatgcaag caactaatca
     4261 agatacgagt atgatgaata ttattcaagc agattggata agccaatacg ctaacacagc
     4321 actttctgaa ctctcggcac aggctaattc tgctctgcaa atcagtaata gcttagatcg
     4381 ccaactcttc aaacaaagcg ataaattcaa cgtatggagc agcgtcgaac atcagaaaac
     4441 cgagcataaa tcagatttat accgcccgta taaacaacaa accaacctga cccaactggg
     4501 catacaaatg ccgatagata acggtttaat gtttggagtt gcattatcta aaaaccacgc
     4561 taacgcggaa tttaacgagg gtgtaaacgg taaatcgaat ctactaatgg caagcctata
     4621 tggtaagtgg caatctcaac aaggcacttt tatcagcctt gatggcagct acggtaaagc
     4681 aaaaaaccaa ctctacctat ttggtgaaaa ccactttacc cgccgaattt cctctattgg
     4741 tgctaacatt ggacatcaat ttgacctcgc aggagttcaa attcagccaa caataggagc
     4801 aagatactac catttcagcg gccaagacta tacactagga ggagcgaaaa tcagctcacc
     4861 aaatacccac tttatgacat atcaagcggg tctaaaagct agtaaaacct tttcattgga
     4921 tgactggaaa gttgaaccaa gcattacaac ccactatgtg gatgcaagta acaaacgctt
     4981 agcagtaaac gtcaataata acaatatcaa ccaacgtttc ggtcgctatc tgaaaaccga
     5041 agctggtatc cgatttgaac ataaccaatg gcaattcgac ttacacgctg gtttcattaa
     5101 cggcaacgaa atcaaaaaac aacattttgc tggtgttaag ctaggatata gctggtaaga
     5161 aaaagcccct agaactattt ctaggggctt tttctactct taaaaagagt atctaacttg
     5221 ccttattttc taaccaattc ttaataaaat ctgctattcc cccaatatta ttaatatcca
     5281 accaattttc tctctcaagc gggtaatccg ttgcggtggc aatcgtccat tggtctagct
     5341 ctggcaatgg tttttcaatc cctttgcggt ggagctgaat ttttggcaag gtttcgtgtt
     5401 taaagccttc tacgaaaacg agatcaacct cttgcgggtt gaattttgca attaattgag
     5461 aaaattcgac cgcttgtttg gtttctgtca ttaatgccca acgttcatcg cacacaatca
     5521 ttgtcgggtt tgctcctgct tcacgtaggc ggtagctgtc tttgcctttt ttatctacat
     5581 caacgttatg gtgggaatgt ttaatcagcc caacccgaat accgcaagcg gtcaattttg
     5641 ggattaattt ttccaataag gtggttttac ctgtgccgct gtagcctgta attgcaaggt
     5701 tttttacagg gaattggctt actcggctta aatcttccaa tgtattcacg ttggtgaatg
     5761 ccgatttttg ttcggaaaaa tcaactgcca cgcttttttg tgattggaag aacgcaagca
     5821 attgctgttg gtttgagcct aaataggctt tcaaatcttc ttgtaccgaa cgatgaatca
     5881 acgcaaaagt gggatgtgga cgctcaccat catgggcata tgcaatttgt acgttattta
     5941 tccgcaaagc agtttttaat ttttgcaata acagctcagg cataaagggc gtatcgcaag
     6001 gcacaaaaag caaatagtca ctttcaattt gctcaaatcc tgccagcatt ccactgagtg
     6061 gaccttgaaa atcaggcaag ctatcttgat agtaagaaaa agcaggataa agctgctgat
     6121 attgctcaat agaacgattg acattaagcc aaatgtcact aacttgtggt gaaagtcggg
     6181 ctaaaatgtg tgaaataagc ggttttcctt caaaaagttg taaccctttt tctaccccat
     6241 tcattcgacg agctaaaccg cccgctaaaa tcacggctga aatgcgttct tctattgttt
     6301 tactcacagc tttatccctt aaaattttgc aaaaataaga gtaaaagtat attatttcgt
     6361 ttaatttcca acaagttttt taaggggtaa aagatgaaat gcgaacgttt agaagagtta
     6421 ttagagttac ttggcgaaca ttggcgtaaa aatcctgact tacacctcat tgatattttg
     6481 cagcagcttt cagttgaagt gggcgagcct gataatttca aagcgttaag cgatgaagtg
     6541 ttaatctatc agcttaaaat gcgaaatgca ggcaaatttg agcctattcc cggcataaaa
     6601 aaagattatg aagatgattt taaaacggct ttattgcgag ctcgtggaat tttaaacgat
     6661 taagattgtc ttatccgtta gctagtttag catttattat ttatcaacat ttttgaagga
     6721 atcattatga aaaaattggc attaaaaacg gcatttgttg cactttctgc cctttttaca
     6781 tttaactcaa cggcaatcgc tgctgatcca accgcaggca aagaatatat cgaagtgcgt
     6841 aaagcacctt ctgcacaaaa agaagtggtg gaatttttct cattctactg cccacactgt
     6901 tatgattttg aattaagcta caaaatccca agccaaatca aagaaaaatt accaagcgat
     6961 agcaaattag tgcaatatca cgttaatttc ttaggtcgcc aatctgaaga tttaactcgt
     7021 gcttgggcat tagcaatggc attaggtgcg gaagataaag taaaaaccgc attatttgaa
     7081 ggtgctcaaa aagacgcatt caaatcaatg gacgatattc gctctgtatt cttagcaaac
     7141 ggcattacag ctgaacagtt tgatagcggt attaatagct ttgcggtaaa tggcttagtg
     7201 aataaacaag ttcaattagc ggaagacttc caaattcgtg gtgtgcctgc attcttcgta
     7261 aatggtcaat accaactcaa cttagaaggc tttgcagatt cttcatcaac gaatgatttc
     7321 atcaaacgtt atgttgatgc ggttgtgttc ttaagcaaga aataacccct taaaatagtt
     7381 agggttttta caatgctgag taggtaaatc ctattcagca ttttttatat gcaaactcgg
     7441 agaacaaaat gacaaagtta tctatcctaa accttgtacc tattcgtgaa gggcaaactg
     7501 ccaaacaagc ggtcgattct atggtgaaat tagcaaaaca cgcggaaagt atcggcattg
     7561 aacgctattg gattgcagag caccacaata tgaaaaacct tgccagctcg gcaacacagc
     7621 tcctcattca gcatgccctt tctcacactg aaaaattaag agtgggatca ggtggcgtga
     7681 tgttgcctaa ccatagtcct tatattgtgg cagagcaata cggtacgctt gaaacactct
     7741 accctaaccg tgtagaactt gggcttgggc gagcaccagg caccgatatg cgaaccgcta
     7801 ccgctttacg ccgtaatgca caaagtctgc catttcctga tgaaatcagc gaactaaaag
     7861 gttattttga aggcacaaat cctgtgtctg cctaccctgc acaggggttg aatgtgccgt
     7921 tttatatttt aggctcaagc cctgaaagtg cctatttagc agcagaacgt ggcttgcctt
     7981 atgcctttgc ttcacatttc gctcctcgtt ttatggacga tgcggtgcga atttatcgca
     8041 cacactttaa gccgtctgaa gtgttagata aaccctatgt gattttaggt gtgaatgcga
     8101 ttgcggcaga aaccgaccaa gaggcagaac agcttgctac cactcagact cagtttttct
     8161 taaatgttgt taccaatgcc caaaactatt tacaaccgcc cgtcgaatcc gatgataaag
     8221 tgtgggaaaa ttttgtgaaa gcggaaatcg taccgcattt cgggcctgtt tcgttccaac
     8281 aaaatgcgtt atacaaccaa gagaaaaccg ttgttcgcca aatgactgct tgttcactta
     8341 tcggtagccg agagagcgtt gaacaacagc tcaagtcatt aaaagcacgc attgaaattg
     8401 atgaaattat ggcggtaagc tacattttcg atgagcaaaa acagcatcaa tcctatactt
     8461 ggttaaaaga gattgcagat aaggtgtgat atgagcaaaa caatgcaaca aaaagccgct
     8521 gatgcacata atatgtgtaa gctaaaaggc gactctatgt tagataactc agaccgccaa
     8581 attgcctttg aagcagtgta tgatagtgaa gaaatagctg tacaagcggt cgaatttttc
     8641 acacaaaaag caaaatcggt agaaaccgag ccttgcgaca ttcaaagtga aatcacaccc
     8701 attatagatg gctttctgat gaaaatgtgg ataactttca gttgccaagc agaagtaatc
     8761 ttatttcaaa tggcggtgag atagatat
//
LOCUS       DANJWC010000098         8759 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.098, whole genome shotgun sequence.
ACCESSION   DANJWC010000098 DANJWC010000000
VERSION     DANJWC010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8759
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.098"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..661
                     /locus_tag="PWX02_001618"
     CDS             <1..661
                     /locus_tag="PWX02_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1729614.1"
                     /translation="TTIVGPNGGGKSTLLKILLKLLSPNKGKVIHQKGLKIGYVPQKL
                     HLEPSMPITVYRFLALKPHTTKEMINQALDLFSITHLTENSMQKLSGGELQRVLLARA
                     ILDRPQLLVLDEPMQGVDIIGQTELYQLLNKTRDLLNCAILMVSHDLNIVMANTDEVL
                     CINKHICCAGSPEVITHDPSFIHFFGDQFARNVAFYSHKHNHHHNLHGDICLGECSCE
                     H"
     gene            826..3639
                     /gene="sucA"
                     /locus_tag="PWX02_001619"
     CDS             826..3639
                     /gene="sucA"
                     /locus_tag="PWX02_001619"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDL1729615.1"
                     /translation="MQYTHFDDWLASSALGGANQSYIEEIYEQYLENPANVDESWRNI
                     FDSFPKTPQFEQAHTPIRDYFRKLAREQQPQSVTIIDPEAGAKQVRLLQWINAHRFRG
                     YLEAKLDPINYYRWKTSSVPELDYKYHGFTDEDLNETFTVGRYVYDKDTMKLSELDKA
                     LKETYLGPIGLEFMHVQDIEQRNWLQAKMESVLNKPLFTKAEKLNLLSELTAADGLER
                     YLGAKFPGAKRFSLEGSDAFIPMMKEIIRHASRQGMTDVVLGMAHRGRLNMLVNVFGK
                     RPAELFDEFAGKHADESRTGDVKYHQGFSSNFAVDDKNVHLTLAFNPSHLEIVSPVVI
                     GSVRARQERMRDTEHEKVLAVTVHGDSAVAGQGVVQETLNMANARGYKVGGTIRIVIN
                     NQIGFTTSNPNDTRSTEFCTDIAKMIQAPIIHVNGDDPEAVAFAARMAVEYRTKFKRD
                     IFIDLISYRRHGHNEADEPLATQPMMYSIIKKHPTPRKVYADRLITEGVLTNEQEIEM
                     LNLYRDALDNGECVVPEWREMDMAAVDWLQYLNYDWTALYESKFPEERFLTIAKRVCE
                     YPASLKAHSRVEKIYTDRKEMYEGKKLFDWGMAETMAYATLLDEGTHVRLSGEDAGRG
                     TFFHRHAVIHNQNDGTGYVPLTQLHANQGRFQVWDSVLSEEAVLAFEYGFATTDPKTL
                     TIWEAQFGDFANGAQIVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARVERY
                     LQLCAQQNIQVCVPSTPAQVYHMLRRQAIRKMRRPLVAITPKSLLRHPLAVSSLDELI
                     NGSFQNVIGELDNIDPKQVKRVVMCSGKVYYDLIEKRRENNQTDVAIIRIEQLYPFPL
                     EEMQQILEPYAHVKDFVWCQEEPLNQGAWYCSKHNFEASLPEDAKLTYAGRPASASPA
                     VGYPSLHAKQQKQLVEDALTIAE"
     gene            3742..4971
                     /gene="odhB"
                     /locus_tag="PWX02_001620"
     CDS             3742..4971
                     /gene="odhB"
                     /locus_tag="PWX02_001620"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDL1729616.1"
                     /translation="MTIEILTPDLPESVADATVATWHKKVGDNVKRDEILVEIETDKV
                     VLEVPASSDGILAEITQEQGATVVSKQSLGKLVVAKAGDISSATIEQKTESTPSDRKH
                     AAIENSHANADDQGPAIRRLLAEHDLQAADIQGSGVGGRITREDIEKEIAKRVQINQV
                     KGSATSQNTVSTVAFNSRSEKRVPMTRLRKRIAERLLEAKNTTAMLTTFNELDMQPIM
                     NLRKTYGEKFEKQHGVRLGFMSFYIKAVVEALKRYPEINASIDGDDVVYHNYFDISIA
                     VSTPRGLVTPVLRDCDKMSMADIEKKIKELAEKGRDGKLTVEDLTGGNFTITNGGVFG
                     SLMSTPIINPPQSAILGMHAIKDRPVAIDGQVVIRPMMYLALSYDHRLIDGKESVGFL
                     VAVKELLEDPTRLLLEI"
     gene            5264..6421
                     /gene="sucC"
                     /locus_tag="PWX02_001621"
     CDS             5264..6421
                     /gene="sucC"
                     /locus_tag="PWX02_001621"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDL1729617.1"
                     /translation="MNLHEYQAKQIFKQYGLPVSQGIACKTAEEAVEAIKQLQGEQWA
                     IKCQIHAGGRGKAGGVKLVRNEAEVREFADKWLGKRLVTFQTDANGQPVNTLYLEETC
                     NIDRELYLGAVVDRSAQKVIFMASSAGGMNIEEVAEKTPELIHKAVIDPLTGGQGYQG
                     RELAFKLGLKGDEVKQFADIFVKLANLFVEKDLALLEVNPLVITKEGQLLCLDAKMSI
                     DSNALYRHPELKELQDPSQDDEREAEAEKWNLNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLYGGKPANFLDVGGGATKERVAAAFKLILSDPNVKAVLVNIFGGIVRCDLIADGVIA
                     AVNEVVNVPVIVRLEGTNAAQGREILANSGLSILAASSLKQAAELAVKSAN"
     gene            6430..7299
                     /gene="sucD"
                     /locus_tag="PWX02_001622"
     CDS             6430..7299
                     /gene="sucD"
                     /locus_tag="PWX02_001622"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDL1729618.1"
                     /translation="MSILIDKNTKVICQGFTGGQGTFHSEQALAYGTQLVGGVSPNKG
                     GTTHLGLPVFNTVREAVEATGATATVIYVPAPGCKDAILEAIDAGIQLIVCITEGIPT
                     LDMLQVKQRLNQTGVRMIGPNCPGVITPDECKIGIMPGNIHKKGNIGIVSRSGTLTYE
                     AVKQTTDEGFGQSTCVGIGGDPIPGSNFIDILKLFQDDPETEAIVMIGEIGGSAEEEA
                     AAFIKENVTKPVVSYIAGVTAPKGKRMGHAGAIISGGKGTADEKFKILEDAGVTTVRS
                     LAEIGSALRKILG"
     gene            complement(7370..>8759)
                     /locus_tag="PWX02_001623"
     CDS             complement(7370..>8759)
                     /locus_tag="PWX02_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDL1729619.1"
                     /translation="DGPLWDVTIILLLGAGLFFTLTTGFVQLRLLPQSLREMWGGRST
                     EGKSLTPFQAFTTGLASRVGVGNIGGVATAIALGGPGAVFWMWITALMGMSSAFAESS
                     LAQLYKTKDPNGMFRGGPAYYITRGLKLPSLAIAFAVTLIFTFGFAFNAVQSNSIVEA
                     TKNAWDWNPVYVGMTLVVITGLIISGGVKRIGKVSAQVVPMMALFYLIIAVIILGMNI
                     EKVPSIISLIIESAFDFSAAAGGMFGALVSKTMLMGIKRGLFSNEAGMGSAPNSAATS
                     DAKHPASQGLIQMLGVFVDTIVVCTCTAVIILMSNNYGGETLRGISLTQSALQFHLGE
                     FGLHFLAFVILLFCFTSIIGNYAYAETNIRYIKNSPWFVWGFRAIVLFFVYFGAVRDG
                     GIVWAFADTVMASMAVINLVAILILAPIVWIILKDYLRQVKSGKEPVFRIEEHQDLIH
                     KGVDPLIWKSKE"
BASE COUNT         2728 a         1903 c         1863 g         2265 t
ORIGIN      
        1 cacgacgata gtcgggccaa atggtggagg aaaatctacc ctgcttaaaa ttttgctaaa
       61 attacttagc ccaaataaag gaaaggttat tcatcaaaaa ggactcaaaa ttggctatgt
      121 accacaaaaa ttgcatttag aaccctcgat gcccattact gtttatcgat ttcttgcgct
      181 aaaaccacat actaccaaag aaatgattaa ccaagcacta gatctgtttt ctattaccca
      241 tctcactgaa aatagtatgc aaaaattatc gggtggtgaa cttcaacgtg tattgctggc
      301 tcgggcaatt ttagatcgtc cgcaactttt agtgttagat gaaccaatgc aaggcgttga
      361 tattataggc caaaccgaac tctatcaatt acttaataaa acaagggatc tccttaactg
      421 tgctatttta atggtctcac acgacttaaa tattgtgatg gcgaatacag atgaagtgct
      481 ttgcatcaat aaacatattt gttgtgccgg aagccccgaa gtcattacac acgatccgag
      541 ctttatccat ttttttggcg accaatttgc acgtaatgtt gcattttatt cacataaaca
      601 taaccatcac cataatttac atggcgatat ctgccttggt gaatgtagtt gtgagcactg
      661 ataggcgtcc tttttaccaa atgtttaaaa tttgtaaaaa taatgctaaa aataaccgct
      721 tgcagcacaa gatttatcta aaaatcagac ttacttcaca ttttatttgc ttaaattgga
      781 gtataataag ggcgtgattt cataccacaa acaggtcagg tctttatgca atatacacat
      841 tttgatgatt ggcttgcatc atctgcatta ggcggtgcaa accaatctta tatagaagaa
      901 atttatgaac agtatctaga aaatccagca aatgttgatg aaagctggag aaacatcttt
      961 gattcatttc ctaaaactcc ccaatttgaa caagctcaca cccctattcg tgactatttc
     1021 cgtaaattag cccgtgaaca gcaacctcaa tccgtcacta ttattgaccc agaagcaggg
     1081 gcgaaacaag ttcgcttact gcaatggatt aacgctcacc gtttccgtgg ctatttagag
     1141 gcaaaactag acccaattaa ctattaccgt tggaaaacct ccagcgtgcc tgaattggac
     1201 tacaaatatc acggcttcac cgatgaagat ctcaacgaaa cttttacggt cggtcgctat
     1261 gtctatgata aagacacaat gaagctcagc gaactggata aagccctaaa agaaacttat
     1321 ttagggccta ttggtttaga atttatgcac gttcaagata ttgaacaacg caactggctt
     1381 caagccaaaa tggaaagcgt gctaaacaaa ccgctgttca ccaaagcaga aaaactgaat
     1441 ttattaagcg aattgaccgc tgccgatggt ttagagcgtt acttaggggc aaaattcccg
     1501 ggggcaaaac ggttctcatt ggaaggcagt gatgccttta ttccgatgat gaaagaaatt
     1561 atccgccacg cttcccgtca aggtatgacc gatgtggttt taggtatggc acaccgtggt
     1621 cgtttgaata tgttagtgaa cgtatttggt aaacgcccgg cagaattgtt tgatgaattt
     1681 gccggcaaac acgctgatga aagccgcacg ggcgatgtga aataccacca aggtttctcc
     1741 tcaaactttg ccgtagatga taaaaacgtt caccttaccc ttgctttcaa cccatcgcat
     1801 ttagagattg ttagcccggt ggtaatcggc tcagtgcgtg caagacaaga acgtatgcgt
     1861 gataccgaac acgaaaaggt gcttgcggtg accgttcacg gtgactctgc agtggcaggg
     1921 caaggtgtag tgcaagaaac cttaaatatg gcaaatgctc gtggctataa agtaggaggc
     1981 actatccgta ttgtgatcaa caaccaaatc ggatttacca cctctaaccc aaatgacacc
     2041 cgctccaccg aattttgtac cgacattgcc aaaatgattc aggcaccgat tattcacgtt
     2101 aatggtgatg atccggaagc tgttgccttt gcagcgagaa tggctgtgga atatcgtact
     2161 aaatttaaac gtgatatttt tattgattta atctcttacc gtcgtcacgg tcataatgaa
     2221 gccgatgagc ctttagcaac tcagccgatg atgtatagca tcatcaaaaa acacccgaca
     2281 cctcgcaaag tctatgcgga tcgtttaatt accgaaggtg ttttaaccaa cgagcaagaa
     2341 atcgagatgt taaacctgta tcgtgatgcc ttagacaacg gcgaatgcgt tgtgccggaa
     2401 tggcgtgaaa tggatatggc agcggtcgat tggctacaat atctcaatta cgactggact
     2461 gccctatacg aaagtaaatt ccctgaagaa cgattcttaa ccattgccaa acgggtgtgc
     2521 gaatatccgg cttcgcttaa agctcattct cgggttgaaa agatctacac tgatcgcaaa
     2581 gagatgtatg aaggcaagaa attattcgac tggggtatgg ctgaaacaat ggcatacgct
     2641 actttgttag acgaaggcac acatgttcgc ctttcaggag aagatgccgg acgtggcact
     2701 ttcttccatc gccatgcggt aatccataac caaaatgatg gaacaggcta tgtgccactt
     2761 actcaactgc acgccaatca agggcgtttc caagtatggg attccgtact ctctgaagaa
     2821 gcggtactcg cctttgaata tggctttgcc actaccgacc cgaaaacact gaccatttgg
     2881 gaagctcaat ttggagattt cgctaacgga gctcaaattg ttatcgacca atttatcagc
     2941 tcaggcgaac aaaaatgggg cagaatgtgc ggtttagtga tgttattacc acacggctat
     3001 gaaggtcaag ggccggaaca ctcctctgcc cgagtggaac gctatttaca gctttgtgct
     3061 cagcaaaata ttcaagtttg cgtgccatca acaccggcac aggtttacca tatgttacgc
     3121 cgtcaagcga tccgcaaaat gcgtcgccca ttggttgcaa tcacaccaaa atcattgcta
     3181 cgccacccac ttgcggtgtc atctcttgat gaattgatta acggttcatt ccaaaatgtg
     3241 attggcgagc tcgataatat cgatcctaaa caagtcaaac gtgtggtgat gtgttcgggt
     3301 aaagtgtact acgacttaat tgaaaaacgc cgtgaaaata accaaacgga cgttgccatt
     3361 attcgtatcg aacagctcta cccgttccca cttgaagaaa tgcaacaaat tctcgagcct
     3421 tacgctcacg ttaaagactt tgtctggtgt caagaagagc cactaaacca aggtgcgtgg
     3481 tattgcagca agcacaattt tgaggcttct ctgccggaag atgcaaaatt aacttacgca
     3541 ggcagaccgg cttctgcttc accggcggtg ggttatccat cgttacacgc aaaacaacaa
     3601 aaacaacttg ttgaagatgc tttaacgatc gctgaataat aaaatgggcg tatatacgcc
     3661 ctacaagcgg ttcactttta accaaatttt acaatttacg ctgtcggagt tttaaaactc
     3721 tctcgaaaaa aggaataagt tatgaccatt gaaattttaa cccccgattt acccgaatct
     3781 gttgccgatg cgaccgttgc cacttggcat aaaaaagtgg gcgacaatgt aaaacgtgat
     3841 gaaattttag tcgaaattga aacagacaaa gtcgttttag aagtaccggc ttcaagcgat
     3901 ggtattcttg cagaaattac ccaagaacaa ggtgctactg ttgtatccaa acaatctctg
     3961 ggtaaattag tagtagctaa agccggtgat attagctctg caactattga gcaaaaaaca
     4021 gaatccacgc catcagaccg caaacacgca gcaattgaaa acagccacgc gaacgctgat
     4081 gatcaagggc ctgcgattcg ccgtttatta gccgaacacg acttacaagc cgctgatatt
     4141 caaggcagtg gtgtaggcgg cagaatcacc cgcgaagata ttgaaaaaga gattgccaaa
     4201 cgagtgcaaa tcaaccaagt aaaagggtct gcaaccagcc aaaacaccgt tagcactgta
     4261 gcatttaaca gccgtagcga aaaacgtgtg ccgatgacac gactccgcaa acgtattgcc
     4321 gaacgcttac tagaggcaaa aaacaccact gcaatgctca ctacttttaa cgagctggat
     4381 atgcaaccaa ttatgaattt acgcaaaacc tatggcgaaa aattcgaaaa acaacacggt
     4441 gtgcgtttag gctttatgtc gttctacatt aaagccgtgg ttgaagcatt aaaacgctac
     4501 cctgaaatca atgcctccat tgatggcgat gatgtggttt atcataacta tttcgacatc
     4561 agcattgcgg tttctacccc acgaggctta gtcacacctg tgttgcgtga ttgcgacaaa
     4621 atgagtatgg cagatattga gaagaaaatt aaagaacttg ccgaaaaagg gcgtgacggc
     4681 aaattaaccg ttgaagactt aaccggcggt aactttacca tcaccaacgg cggggtattc
     4741 ggttcattaa tgtccacccc aattatcaac ccgccacaaa gtgcgatttt gggtatgcac
     4801 gctattaaag accgccctgt cgccattgac ggacaagtag tgatccgccc gatgatgtac
     4861 ctagccttaa gctatgacca ccgtttaatt gacggtaaag aatctgtcgg tttcttagtg
     4921 gcagttaaag agttattaga agacccaacc cgtcttcttt tggaaattta atcgaaaata
     4981 tgaccgcttg tacttttagc tacaagcggt ctgtttttaa taaaatttga caaaatccga
     5041 taaacgctct atttgagttc ccttctgagc cacctgagcg actttgaatt tgtaggaaaa
     5101 atttagtgtt tgagcttaca aggcaagcga gttataaatt tttccgtcaa gcaaattcaa
     5161 actaagcgaa gaaagtggcg aagcagggtg tgctttcttt tggttacttt tctttgcaca
     5221 agcaaagaaa agtaacataa aaaaaaccaa acgaggaaac tctatgaatc tacacgaata
     5281 tcaagcaaaa caaatattca aacaatacgg gcttcccgta agccaaggga ttgcttgcaa
     5341 aacggcagag gaagccgttg aggcaattaa acaactacaa ggtgaacaat gggcgataaa
     5401 gtgccaaatt cacgctggtg ggcgtggcaa agccggcggg gtgaaactgg ttcgcaatga
     5461 agccgaagtg cgtgaatttg ccgataaatg gctcggcaaa cgcttagtta ccttccaaac
     5521 cgatgctaat gggcaaccgg ttaatacctt atatttagaa gaaacctgca atatcgaccg
     5581 tgaattgtac ttaggtgcag tggttgatcg ctccgctcaa aaagtgattt ttatggcatc
     5641 aagtgccggt ggtatgaata ttgaagaagt agcggagaaa acccctgaat taattcacaa
     5701 agcggtaatt gacccactca ccggtgggca gggttatcaa gggcgtgaat tagcatttaa
     5761 attaggcttg aaaggtgatg aagttaaaca atttgcggat attttcgtga aactggctaa
     5821 cctatttgtt gaaaaagatt tagctttatt agaggtaaat ccacttgtca tcactaaaga
     5881 agggcaacta ctctgcttag atgcaaaaat gtcgattgac agcaatgctc tctaccgcca
     5941 cccagaactt aaagagttgc aagacccatc gcaagatgac gaacgtgaag ctgaagcgga
     6001 aaaatggaat ctcaactacg ttgcactaga tggcaatatc ggctgtatgg taaacggtgc
     6061 ggggctggca atggggacga tggatatcgt caaactttat ggtggtaaac cggctaactt
     6121 cttagacgtt ggcggtggtg caaccaaaga gcgtgtagca gcagctttta aattgatttt
     6181 atctgatccg aatgtgaaag ccgtgttagt caatattttt ggtggtatcg tccgctgcga
     6241 tttgattgct gatggcgtca ttgctgctgt aaacgaagtc gtgaatgtgc ctgttattgt
     6301 gcgtttagaa ggcactaatg ccgcacaggg gcgtgagatt ttagccaata gcgggctgag
     6361 tattctagcg gcatcatcat taaaacaagc ggctgaatta gcggtaaaat ctgcaaatta
     6421 ggaggaaaaa tgtcgatttt aattgataaa aacacaaaag taatttgcca aggtttcacc
     6481 ggcggacaag gtactttcca cagcgaacaa gcccttgctt atggcacaca attagtcggc
     6541 ggagtttcgc cgaataaagg tggcacaacc catttaggct taccggtttt caacaccgta
     6601 cgcgaagcgg tagaagcaac cggtgcaact gcaaccgtta tctacgttcc ggctcccggc
     6661 tgtaaagatg ctattttaga agccattgat gccggcatcc aactaattgt ctgtattact
     6721 gaaggcattc caaccttgga tatgctacaa gttaaacaac gtctaaacca aaccggcgtg
     6781 cgaatgatcg gtccgaactg cccgggtgtg attacccctg atgagtgcaa aatcggcatt
     6841 atgccgggca atatccacaa aaaaggcaat atcggtattg tttctcgttc gggtacttta
     6901 acctatgaag cagttaaaca aacaactgac gaaggctttg gtcaatctac ttgtgtcggt
     6961 atcggtggcg atccgattcc gggatctaac tttattgaca ttttaaaact gttccaagac
     7021 gatcctgaaa ctgaagccat tgtgatgatt ggtgaaatcg gcggctctgc cgaagaagaa
     7081 gcggcagctt tcatcaaaga aaatgtcacc aagcctgtag tcagctatat tgccggtgtc
     7141 accgcaccaa aaggcaaacg aatgggacac gccggtgcga ttatcagtgg cggcaaagga
     7201 accgccgatg aaaaattcaa aatccttgaa gatgccggtg tgacaaccgt cagaagttta
     7261 gccgaaatcg gttcagctct tagaaaaatt ttaggctaaa ctaaaagttc aagcggtcat
     7321 atttacccat ttttttgtaa atatgaccgc ttgttttttt gctattaaat tactctttcg
     7381 atttccaaat taatgggtca acacctttat ggataaggtc ttgatgttct tcaattctaa
     7441 acactggctc tttgccactt ttcacttggc gaagatagtc ttttaaaata atccagacaa
     7501 tcggagcaag aattaagata gccaccaagt taattaccgc cattgatgcc atcactgtat
     7561 cggcaaatgc ccatacaatc cctccgtctc ttactgcacc gaagtacaca aagaataaaa
     7621 cgatggcacg gaatccccac acaaaccacg gactgttttt aatgtagcgg atattagttt
     7681 ctgcataagc atagttaccg ataatggatg tgaagcaaaa cagtaaaatc acaaatgcta
     7741 aaaagtgtaa accaaattca cctaaatgga attgtaacgc actttgagta agtgaaatac
     7801 ctcttaaagt ttcaccacca tagttatttg acataagaat gatcactgca gtacaagtac
     7861 agactacaat tgtatctaca aatacgccca acatttgaat taagccttga cttgccgggt
     7921 gtttcgcatc agaagtcgct gcagaatttg gtgcagatcc cataccagct tcgtttgaga
     7981 acaagccacg tttaatcccc attagcatag ttttggatac caatgctcca aacattccac
     8041 ctgcagcagc agaaaaatca aaggcgcttt caataattaa agagattata cttggcactt
     8101 tttcaatatt catacctaaa ataattactg caataatcaa gtagaataat gccatcattg
     8161 gaacaacttg agcagaaact ttaccaatac gcttgacccc tccagaaata attaaccctg
     8221 taatcaccac taatgtcatg cctacgtaga ctgggttcca atcccacgca tttttggttg
     8281 cttccacaat agagttagat tgcacagcat taaaagcaaa accaaaggtg aagataagcg
     8341 tcactgcaaa agcaatagca agagaaggca gttttaaacc acgagtgatg taataggctg
     8401 gcccaccacg aaacattccg tttggatctt tggttttata caactgagcc agtgaagact
     8461 cagcaaaggc ggaactcatt cccattaagg cggtaatcca catccagaat accgcacccg
     8521 gcccacccaa agcgattgca gtagctaccc caccaatatt accaacccca acacggctgg
     8581 ctaatccagt agtaaaggct tggaacggag ttaatgattt cccttccgta gaacgtcctc
     8641 cccacatttc acgtaaactt tgcggaagaa gacgcagctg gacaaaacct gttgtaagag
     8701 tgaaaaataa ccctgcaccg agcaatagga taatcgttac atcccaaagt ggaccgtcg
//
LOCUS       DANJWC010000099         8748 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.099, whole genome shotgun sequence.
ACCESSION   DANJWC010000099 DANJWC010000000
VERSION     DANJWC010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8748
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.099"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..218
                     /locus_tag="PWX02_001624"
     CDS             <1..218
                     /locus_tag="PWX02_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HDL1729620.1"
                     /translation="IYTDFLSREEIEFITQCKQKNLIKKQLNIMGIPFKENANGFPVV
                     RRDYAQAKQRKPIATNDSDWVSNAFKA"
     gene            240..1280
                     /locus_tag="PWX02_001625"
     CDS             240..1280
                     /locus_tag="PWX02_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL1729621.1"
                     /translation="MARPRKYENNGLPQNLLCRRRKRGNGQIVEYYFYVLSNGKEKSL
                     GTNKYEAVLEAAKLNFENSKKSPVVLFIDVAKRYELEIVPTKKAKNTRQSNLQAIHWL
                     CKFFGNPPAPLEKIEPKHISQYLQWRKDTPAVANIEVGLFNTIWNMAREWGYTTLPSP
                     SQGVKKFPTKYREVYVEDYILDKIYEFADERMADIIETAYLLGQRPIDICNIHHSHIY
                     DGILHITQQKTGKKVRFEISGRLKEILDKRLGYESDWIFTNKWGKKLKRRSLGDHFKE
                     IKERAMKAYPELADEIAKVQMRDMRAKAATDISLMTTDEQAQKQLGHTSKRMTHHYIR
                     KDKILKPTDEIT"
     gene            complement(1439..1726)
                     /gene="rplY"
                     /locus_tag="PWX02_001626"
     CDS             complement(1439..1726)
                     /gene="rplY"
                     /locus_tag="PWX02_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HDL1729622.1"
                     /translation="MSFKFEAEVRSAQGKGASRRLRHNGQVPAIIYGGNAEPVSIILD
                     HDKVNNAQVHDSFYNEVLTIVVAGKEEQVKVQAIQRHPTKPKLVHLDFKRA"
     gene            complement(1878..3146)
                     /locus_tag="PWX02_001627"
     CDS             complement(1878..3146)
                     /locus_tag="PWX02_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HDL1729623.1"
                     /translation="MSDLKQQALDFHEFPIPGKISVTPTKALDTQHDLALAYSPGVAE
                     PCLAIEKDPSASYRYTARGNLVAVISNGTAVLGLGNIGALASKPVMEGKGVLFKKFAG
                     IDVFDIEINETDPEKLVEIIASLEPTFGGINLEDIKAPECFYVEQKLRERMRIPVFHD
                     DQHGTAIITGAAVINGLQVVGKAISEVRLVVNGAGASAIACTNLLRSLGVKKENITMC
                     DSKGVIFQGRGDKMDETKQFYAIEDNGWRTLADAVANADVFLGCSKGGALTQEMVKTM
                     AKDPLILALANPVPEITPAEAKAVRPDAIVCTGRSDFKNQVNNVLCFPFLFRGALDVG
                     AKTINEEMKMAAAHAIAELAKEPVPFEVISTYGELSFGPEYIIPTPFDPRLIVNVSIG
                     VAKAAIQSGVATRPITDWEAYAEQLKKLVA"
     gene            complement(3303..4214)
                     /locus_tag="PWX02_001628"
     CDS             complement(3303..4214)
                     /locus_tag="PWX02_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HDL1729624.1"
                     /translation="MKWLMLCREPRLYSCCRIKAACEAKGIELDILDPNRMLLGLENG
                     EFKIYYQAGESYDNNRPEPVLLPNYDGILPRFGTGSTEMGCSVLRHFEAKGIPVLNHS
                     AAFALARDKWRTLQKLAKHNLPVPNTNFAGHLVSVKSQLNQFAFPLISKVLNGSQGNG
                     VMLFEGKNNAEAVLATFRQVNEPYLCQQFVGEAKGQDIRAFVIGNEVVAAMSRTSVTG
                     DFRANIHQGGTAQPIELTQMERALAIKATKTIGLDIAGVDFLRTEKGVVILEVNASPG
                     FEGIERVNDVDIASEMVSYFINKMKNA"
     gene            complement(4211..4930)
                     /gene="nfsA"
                     /locus_tag="PWX02_001629"
     CDS             complement(4211..4930)
                     /gene="nfsA"
                     /locus_tag="PWX02_001629"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NADPH nitroreductase"
                     /protein_id="HDL1729625.1"
                     /translation="MQSKPTLETILSHRSIRKFTEQPISEEIFQTLIEAGKAASSSNH
                     LQCVTVIRVTDRQIRETLKAVSGMQYVVDCAEFLVFCIDFNKHKQLVPESQLEWAEVS
                     LIGAVDTGIFAQNVFLAAESLGLGAVYIGALRNDIERVSKALNLPEYCVPLVGMCLGY
                     PAQEPAIKPRLPNSLVCYNNQYQDFDWTEFAKYNENLTAYYQARIGEPQQWQAAIHKT
                     LDKPVRPHILPFFNQKGLLKK"
     gene            complement(4942..5727)
                     /gene="mtgA"
                     /locus_tag="PWX02_001630"
     CDS             complement(4942..5727)
                     /gene="mtgA"
                     /locus_tag="PWX02_001630"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HDL1729626.1"
                     /translation="MEQSMAKRNKRFLSSLLKKIGLFFIPRKIKSKLGWLWFGSSRLL
                     TFGGTFFLIPILIFSVLPVPYSAYMVQKKVENLVQGKSYPIKKEWVSLNEISWQMQMA
                     VIASEDQKFESHFGIDLQAIETALKLNAKSKKVRGGSTISQQTVKNMFLWHGQSWLRK
                     GIEVPLTLLVENVWSKDRILEVYLNIAEFGNGIFGVEAAAKHFFKKSAKQLTLQESAL
                     LAASLPNPLVFRVDKPGPSMRKRQAWIMRQVSLLDGKNYLDKL"
     gene            complement(5702..6022)
                     /gene="trpR"
                     /locus_tag="PWX02_001631"
     CDS             complement(5702..6022)
                     /gene="trpR"
                     /locus_tag="PWX02_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HDL1729627.1"
                     /translation="MKTLYNQRDPNEWTQFLALLEQAVAEDKLEPFFSMFLTADERGS
                     LGLRLQIVQALLKGEDSQREIQQNLNTSAATITRGSNMLKTLDQDFLQWVNGKVNGTV
                     NGKA"
     gene            complement(6102..6743)
                     /gene="lolA"
                     /locus_tag="PWX02_001632"
     CDS             complement(6102..6743)
                     /gene="lolA"
                     /locus_tag="PWX02_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HDL1729628.1"
                     /translation="MKTLFKKSVFGLALFGLANVALADAQSVAELQRRLEQVSQYSAD
                     FDQTVRSSKGKQIQQGKGKFQVKRPNLFRMDTKSPQENLIVSDGKDLWFYDPFVSQVT
                     VNTVQDAVNNTPFVLLTSSDKSHWDQYDVSQSADTFVLKPKSKKSNLKQFDVRIDQSG
                     LLKGFSTIERDGQSNLYVLRNITTGGVSSDLFKFSVPKGAELDDQRGGKKSKK"
     gene            complement(6854..>8748)
                     /gene="polA"
                     /locus_tag="PWX02_001633"
     CDS             complement(6854..>8748)
                     /gene="polA"
                     /locus_tag="PWX02_001633"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDL1729629.1"
                     /translation="ANVVEIDRSKYETVDTEQKLSAWIEKLTAARLIAVDTETDSLDA
                     MSANLVGISFALENGEACYIPLGHKQKAQPQQADMFGDEERSESPSSDELVKNQLNLT
                     ACLSKLKPILLNPEIKKIGQNIKYDLTIFANYGIGLQGVAFDTMIESYTQNSTGRHNM
                     DDLAARYLGHKTIPFEELAGKGKNQLTFDKIEIAKAAEYAAEDADVTMKLHQVLWEKL
                     QQEPELVKLFDEIEMPLVGVLSRIERNGVLIDPKKLLTQSAEIEKRLEEVETLVHAEA
                     GQTFNLASTKQLQEILFEKLGLPVLKKTPKGAPSTNEEVLDELAQQGHLVPKLLMEHR
                     GLSKLKSTYTDKLPQLINKKTGRVHTSYNQAVTATGRLSSSDPNLQNIPIRNEEGRRI
                     RQAFVAREGYKIVAADYSQIELRIMAHLSNDEGMINAFAEGKDIHRATAAEIFGVELG
                     EVTSEQRRSAKAINFGLIYGMSSFGLSNQLGIGRAEAQKYMDLYFQRYPAVQQFMTDI
                     REVAVEKGYVETLFGRRLYLPDIKSGNAILRKAAERVAINAPMQGTAADIIKVAMIGI
                     DNAIRDNDEIKMIMQVHDELVFEVKADKIDHYSQLIKTEMEKAISLKVPLIAEVGVGE
                     NWDEAH"
BASE COUNT         2577 a         1972 c         1603 g         2596 t
ORIGIN      
        1 acatctacac tgacttcctt tctcgtgaag agattgaatt tattactcaa tgcaagcaga
       61 aaaatctaat caaaaaacaa ctcaacatta tgggcattcc atttaaagaa aatgcaaatg
      121 gattccctgt tgtccgccgt gattatgctc aggctaaaca gcgtaaacct attgcaacga
      181 atgattctga ctgggtatca aatgcattta aagcgtaaat tgagaagaag gaggcactta
      241 tggcacgccc tcgaaaatat gaaaacaacg gattgccaca aaacctactc tgtcgtcgca
      301 gaaagcgtgg gaacgggcaa atcgtggaat actactttta cgttctctcc aatggcaaag
      361 aaaaatccct gggcacaaat aaatatgaag ccgttttaga agcagctaaa ctcaattttg
      421 aaaatagcaa aaaatcccca gtcgttttat ttattgatgt agctaaacgc tacgagctgg
      481 aaattgtccc taccaaaaaa gccaaaaaca ctcggcaatc taatttacag gctattcatt
      541 ggctatgtaa attctttggt aacccacctg caccgctcga aaaaattgag ccaaagcaca
      601 tcagtcaata tttacaatgg agaaaagaca cgccggctgt tgctaatatt gaggtaggtt
      661 tatttaacac aatttggaac atggcaagag aatggggata cacaacactt ccaagtccat
      721 cgcaaggtgt gaaaaaattc ccaactaaat atcgtgaggt ttatgtagag gattatatcc
      781 ttgataaaat ctatgaattt gcagatgaac gaatggcaga tattatcgag acagcgtact
      841 tgctaggaca aaggccgatt gatatttgca acatccacca ctcacacatc tatgacggca
      901 tactgcacat tactcagcag aagacaggga aaaaagtgag atttgaaatt agtggtcggc
      961 taaaagaaat tttagataaa agattgggat acgaatcaga ctggattttt accaataaat
     1021 ggggaaaaaa attaaaacgg cgaagtttgg gcgatcattt taaagaaata aaggagagag
     1081 caatgaaagc atatcctgaa cttgctgatg aaatcgctaa agtacaaatg cgtgatatgc
     1141 gtgccaaagc agccaccgat atttccctaa tgacaaccga tgaacaagct caaaaacaac
     1201 ttgggcatac atcaaaaaga atgacacatc attacatcag aaaagacaaa atcctcaaac
     1261 ctaccgatga aatcacctaa aaataaagcc cctttgccaa agacaaaaga gccatttaat
     1321 cgcctaaaat ccgttccgaa actattttgt aaataattga ttttattgac ctaaaaaact
     1381 ataaaatcgc aaaaagtttc ggaacagaaa acagcgttaa acccttttaa atcaagaatt
     1441 aagcacgttt gaagtctaag tgaactaatt ttggtttagt tgggtggcgt tgaattgctt
     1501 gtacttttac ttgctcttct ttacctgcaa caacgatagt taatacttcg ttatagaatg
     1561 aatcgtgaac ttgtgcgttg ttcactttat cgtgatctaa aatgattgat acaggctctg
     1621 cattaccacc gtagatgatt gcaggaactt gaccattgtg acgcaggcgg cggctcgcac
     1681 ccttaccttg cgcagaacga acttcagctt caaatttgaa tgacatagtt ttattctctt
     1741 aaataaaatg gggaaatccc cgtgaataga aaaaacagca ggcgacccag ctgtttccga
     1801 aaaaatatcg gcatatccgt ctcgatatgc cagctacgta ttttactgaa gttgcagaaa
     1861 aatgcaatgt aaaaatatta agcaaccagt tttttcaact gctctgcata agcttcccaa
     1921 tctgtaattg gtcgagttgc tacacctgat tgaattgctg cttttgctac accaatcgag
     1981 acattcacga tcaaacgtgg atcaaatggc gttggaataa tatattccgg gccaaacgat
     2041 aattcgccat aagttgaaat aacttcaaac ggcacaggtt ctttagccaa ctctgcaatc
     2101 gcatgggctg ctgccatttt catttcttca ttaatggttt ttgccccaac atctaatgct
     2161 ccacggaata agaacgggaa acaaagcaca ttattcactt ggtttttaaa gtctgaacga
     2221 ccggtacata caatcgcatc tggacggaca gccttcgctt cagcaggtgt aatttccggc
     2281 acagggttcg ctaaagcaag aattaatgga tcttttgcca ttgttttaac catttcttgc
     2341 gttaaagctc cgccttttga acaacctaag aaaacatctg cattcgcaac ggcatctgcc
     2401 aatgttctcc aaccattatc ttctattgca tagaattgtt tggtttcatc catcttatca
     2461 ccacgacctt ggaaaataac gcccttagaa tcgcacattg tgatattttc ttttttcaca
     2521 cctaatgaac ggagtaagtt agtacacgca atcgctgatg ctccagcacc gtttaccact
     2581 aaacgtactt ctgaaattgc tttacctacc acttgtaatc cgttaatcac cgccgcaccg
     2641 gtaataattg ccgtaccgtg ttgatcatcg tggaacacag ggattctcat acgttcacgt
     2701 aatttttgct ctacataaaa acattcaggt gctttaatat cttccagatt aatgccacca
     2761 aaagtcggtt ctaacgatgc aataatctcc actaatttct ccggatcagt ttcattaatt
     2821 tcaatatcaa acacatcaat tccggcaaat ttcttaaata gaacaccttt gccttccatc
     2881 acaggcttac ttgctaaagc accaatattg cctaagccta acactgctgt accattagaa
     2941 attactgcaa ctaagttacc tcgtgccgta taacgataag aagctgaagg atctttttca
     3001 atcgctaagc aaggctcagc aaccccagga gagtacgcta aagctaaatc gtgctgagta
     3061 tctaatgctt tagtaggagt aacggaaatt tttccgggga ttgggaactc gtgaaaatca
     3121 agcgcctgtt gttttaaatc gctcatttta gaaaccttat aatctataaa aataaaagta
     3181 atcaataata gccaaagagt agccatttta ccctaaattt tttcgcattt ttatggcttt
     3241 gctctcattt tttatgatat ttacaaaaaa taatacaaaa acgtccgctt gttttactca
     3301 aatcaagcat tcttcatctt attgataaaa taagaaacca tttcagaagc tatatcaaca
     3361 tcatttactc tctctatgcc ttcaaatcct gggctggcat tgacttctaa aataactacg
     3421 cctttctctg tccgcagaaa atcaacgcct gcaatatcaa gccctatcgt tttagttgct
     3481 ttaattgcta acgcacgttc catctgagtt aattcaatcg gttgagccgt tccaccttga
     3541 tgaatattgg ctctaaaatc acctgttacg ctggttcggc tcatggcagc aaccacttca
     3601 ttgccaatca caaacgcccg aatatcttgc cctttagctt cgcccacaaa ttgttggcat
     3661 agataaggct catttacttg cctaaaggtc gctaacacgg cctcggcatt atttttgcct
     3721 tcaaatagca ttacgccatt cccctgtgaa ccatttagca cttttgaaat aagtggaaaa
     3781 gcaaattgat tgagttgtga tttcacagaa accaaatgcc ctgcaaaatt cgtattagga
     3841 accggcaaat tgtgtttggc taatttttgt aatgttcgcc atttgtctcg ggctaacgca
     3901 aatgccgctg aatgatttag aacaggaata cctttcgcct caaaatggcg taacaccgaa
     3961 cagcccattt cggtactgcc tgtgccaaaa cggggtaaaa tgccgtcata attcggcaac
     4021 aataccggct ccggacggtt gttatcatag ctctcgccag cttgatagta gatcttaaat
     4081 tcgccatttt ctaatccgag tagcattcga ttcgggtcta aaatatccag ctcaatcccc
     4141 tttgcctcac aagcggcttt tattcgacag cagctatata aacgaggctc tcgacatagc
     4201 attagccact tcattttttc agcaatcctt tctgattgaa aaacggcaaa atatgcggac
     4261 gaacaggctt atccaatgtt ttatgaatag ctgcttgcca ttgttgtggc tcgccgattc
     4321 tggcttggta gtaagcggtc aaattttcat tatattttgc aaattcagtc caatcaaaat
     4381 cctgatattg attgttataa caaaccaaac tattcggcaa acgaggtttt attgccggct
     4441 cttgtgccgg ataccccaaa cacataccca ccagtggaac acaatattcc ggcaagttaa
     4501 gggctttaga aacccgctca atatcattac gcaatgctcc aatatacacc gctcccagcc
     4561 ctagtgattc ggcggctaaa aatacattct gggcaaaaat accggtatct accgccccaa
     4621 tcaacgacac ctccgcccat tccaactgcg attcagggac aagttgtttg tgtttattaa
     4681 aatcaatgca gaacaccaaa aattcagcac aatctactac atattgcata cctgaaacgg
     4741 cttttaatgt ttctcgaatc tgccgatcgg ttacacgaat caccgtcaca cattgcagat
     4801 gatttgaact tgaagccgct ttacctgcct caattaaggt ttggaaaatc tcttcagaaa
     4861 taggctgttc ggtaaattta cgaatagaac ggtgagataa aatcgtctca agagtcggtt
     4921 tactttgcat atttttctcg tctaaagttt atctaaataa tttttgccat caagcaaact
     4981 tacttgtcgc ataatccacg cttggcgttt tcgcatactc gggccgggtt tatctaccct
     5041 aaacaccaac gggttaggca aagaggctgc caatagtgcg gattcttgca aggttagctg
     5101 tttggctgat tttttgaaaa aatgctttgc ggctgcttcc accccgaaaa tgccgttacc
     5161 aaattccgca atattgaggt acacctctaa aatccgatcc ttactccaca cattttcgac
     5221 taacagggtt aaaggcactt caatcccttt gcgtaaccag ctttgcccgt gccacaaaaa
     5281 catatttttt accgtttgtt gcgaaatggt tgaacctcca cgcacttttt tggatttagc
     5341 attcagtttc aaggcagttt ctatcgcttg taagtcaatg ccaaaatggc tttcaaactt
     5401 ttgatcttct gaggcaatca cagccatttg catttgccac gaaatctcat tcaggctcac
     5461 ccattctttt ttgatcgggt aagatttccc ctgaaccaga ttctccacct ttttttgcac
     5521 catatacgca gaataaggca ccggtaacac cgaaaaaatc agtatcggaa tcaggaaaaa
     5581 tgtgccacca aaggttaata agcgactaga accaaaccaa agccagccca gtttactttt
     5641 tattttgcga ggaataaaaa aaagccctat tttcttcaaa agagagctta aaaatcgctt
     5701 gttacgcttt gccattgact gttccattaa ccttgccgtt cacccattgc agaaaatctt
     5761 ggtcgagtgt tttaagcata tttgaaccac gagtgatggt tgccgcactg gtatttaaat
     5821 tttgttggat ttcccgttgc gaatcttcac cttttagcaa agcttgcaca atttgtaaac
     5881 gcaagcctaa tgaacctcgc tcatcggcgg tgagaaacat tgagaaaaac ggctcaagtt
     5941 tatcttccgc aaccgcttgt tcaagcagag ccaaaaactg cgtccactcg tttggatctc
     6001 gctggttata gagtgttttc atactgtccc ttgaagtaaa aacggtgaca agcggtcaga
     6061 tttttacaaa attttgcaga aaaacgaccg cttgctctcc attatttttt cgatttttta
     6121 ccgccacgtt ggtcgtctaa ctctgcccct ttcggtacgc tgaatttaaa caaatcgctt
     6181 gagacaccgc cggtagtaat attgcgtaac acatataagt tactttgccc gtcacgctca
     6241 atggtgctga aaccttttaa taaaccgctt tggtcaatcc gcacatcaaa ctgttttaaa
     6301 ttgctttttt tcgattttgg cttaagcacg aaagtatcag cactctggct gacatcatat
     6361 tgatcccagt ggcttttatc actgctggtt aataagacga atggcgtatt attaactgca
     6421 tcttgcacgg tatttaccgt aacttgtgaa acgaaaggat cgtaaaacca taaatctttg
     6481 ccgtcagata caatcaaatt ttcttgtggt gatttggtat ccattctaaa taagttcggg
     6541 cgttttactt ggaatttgcc tttgccttgc tgaatttgtt tacctttgct agaacgcacg
     6601 gtttggtcga aatccgcact atattggctt acttgttcca aacggcgttg taattctgca
     6661 acagattgag catcagccaa tgccacattt gccaagccaa ataacgctaa accgaatacc
     6721 gattttttaa ataatgtttt catttgtttt cctttttacc aacaagaatt ttcttgtttg
     6781 acaataaaat catcaaaagt ttcatattaa tgaactgtta caacaataca ataaaagtcg
     6841 tgaattgtat agtttaatga gcttcgtccc agttttcgcc tacgccaact tcggcaatca
     6901 gtggcacttt gaggctaatc gctttttcca tttcggtttt gattaattgg ctgtaatggt
     6961 cgattttatc ggctttgact tcaaacacca attcatcgtg aacctgcata atcattttga
     7021 tttcatcatt atcacgaatc gcattatcaa taccaatcat cgccacctta ataatatcgg
     7081 cagccgtgcc ttgcattggg gcgttgattg ccacccgttc tgctgcttta cgcaaaatag
     7141 cattgccgga tttaatatca ggcaagtaca aacggcgacc aaatagggtt tcaacatagc
     7201 ctttttcaac tgcaacttca cgaatatcgg tcataaattg ttgaaccgcc gggtaacgtt
     7261 ggaagtagag atccatatat ttttgagcct ctgcacgccc aatccctaat tggtttgaaa
     7321 gcccaaatga cgacattccg taaatcagcc cgaagttaat tgctttcgca ctgcggcgtt
     7381 gttcggaggt gacttcacct aactccaccc cgaaaatttc ggcagcggta gcacggtgaa
     7441 tatctttgcc ctccgcaaag gcgttaatca ttccctcatc attcgataaa tgagccataa
     7501 tccgtagctc aatttgtgaa tagtcggcag cgacaatttt ataaccttct ctcgcgacaa
     7561 aagcttggcg aatccgtctg ccctcttcat tgcggatcgg aatgttctgc aaattcggat
     7621 cgctggaaga aaggcgtccg gttgcggtga ctgcttgatt gtaagaggta tgcacccgcc
     7681 ctgttttttt gttaatcagt tgtggtaatt tatcggtgta ggtagatttc aacttactta
     7741 acccacgatg ctccattaat aacttcggca ctaaatgccc ttgttgagcc aactcgtcta
     7801 acacttcttc gttggttgag ggtgcacctt ttggggtttt cttcaacacc ggcaagccta
     7861 atttttcaaa caaaatctct tgtaattgtt tagttgaggc aagattgaag gtttgccctg
     7921 cttctgcgtg tactaatgtt tcaacttcct ccaaacgttt ttcaatttca gccgattggg
     7981 tcaataattt tttcgggtca atcaacacgc cattgcgttc aattcgagaa agtacgccca
     8041 caagtggcat ttcaatttca tcgaataact tcaccaattc aggctcttgc tgtagttttt
     8101 cccacaatac ttggtggagt ttcatcgtca catcggcatc ttctgccgca tattcagccg
     8161 cttttgctat ctcgatttta tcgaaggtca gttgattttt ccccttacca gcaagctctt
     8221 caaacggaat cgttttatgc cccaaataac gggcggctaa atcgtccata ttatgacgac
     8281 cggtgctgtt ttgggtgtaa gattcaatca tcgtatcaaa agcaacacct tgtagcccaa
     8341 tgccgtagtt cgcaaaaatg gttaaatcat atttaatatt ctgcccgatc tttttaatct
     8401 ccgggttaag caaaatcggc ttgagtttac ttaaacaagc ggtcaaattt agctgatttt
     8461 ttaccaattc atcggaagac gggctttctg aacgctcctc atcaccgaac atatcggctt
     8521 gttgcggttg ggctttttgc ttatgcccaa gcggaatata gcacgcctcg ccattttcca
     8581 gtgcaaagga aataccgacc aaattagccg acattgcgtc aaggctgtcg gtttcggtat
     8641 ccaccgcaat caaacgagct gcggtgagtt tttcgatcca ggctgataat ttctgttcag
     8701 tatcaacggt ctcatatttg ctacgatcga tctcgaccac atttgcaa
//
LOCUS       DANJWC010000100         8741 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.100, whole genome shotgun sequence.
ACCESSION   DANJWC010000100 DANJWC010000000
VERSION     DANJWC010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8741
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.100"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            1..1062
                     /locus_tag="PWX02_001634"
     CDS             1..1062
                     /locus_tag="PWX02_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL1729630.1"
                     /translation="MAIYAIWNNKGGVGKSYLTFQIACEYAKQYPAKKVLVVDLCPQA
                     NSSSMLLGGLEKGEQELAKIHSQVNKNTISGYISERLVSPYRSINSTTKYVTQVSTIN
                     EYVPKNVYLVVGDEELEIQSSGILHASQAVHVPDSWRLVHLWVRDLVSDIQNAWDNDD
                     NVVFIDCNPSFTIYTELALAASDRLIIPFSADGSSKRAVKSVLSLVYGVKRHSGDVSS
                     HFYTESQKSKLALPQIYMYVGNRLTINAKASATAFKTVVEEIGDEIYNIWKTNANLFC
                     IHLAGVGTPTNKSSFRKMFQYEVRDANTASVVSGALGIPISRLSSGKRDVLGKNVMVN
                     QSQLDAQVPNIQNLVQCIE"
     gene            1213..2865
                     /locus_tag="PWX02_001635"
     CDS             1213..2865
                     /locus_tag="PWX02_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDL1729631.1"
                     /translation="MTIFQVAQNWLAQDPDAETRAELEQLIQAAESDEKAKAELTARF
                     DGRLQFGTAGLRGRLQAGSMGMNRVLVAQAAGGLAGFIKGYDKEPSIVIGYDGRKNSD
                     VFARDTAEIMAAAGIKTYLLPRKLPTPVLAYAIQYFDTTAGVMVTASHNPPEDNGYKV
                     YLGKANGGGQIVSPADKEIAALIDKVAAGDIRDLPRSQDFTVLDDEVVNAYIEKTASL
                     AKWPKAEISYVYTAMHGVGYEVLSKTLEKAGLPQPYLVSEQIQPDGSFPTVNFPNPEE
                     KGALDLAIKLAKEKNAEFIIANDPDADRLAVAVPDAQGNWKPLHGNVIGCFLGWYLAK
                     QFHAQGKQGVLACSLVSSPALAEIAKKYGLSSEETLTGFKYIGKVENLLFGFEEALGY
                     LVDPDKVRDKDGISAAIMFLDLVCSLKQEGKTLADYTTEFVQEFGAYVSGQISIRVCD
                     LAEIGKLMTALRNNPPSEIGGFKVAEFIDHTKTTRQNDILVFVLENGSRLIARPSGTE
                     PKIKFYLDARGTDAENAEQVLAQFDESVRSLLRQEEYGKQDC"
     gene            2918..3850
                     /locus_tag="PWX02_001636"
     CDS             2918..3850
                     /locus_tag="PWX02_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDL1729632.1"
                     /translation="MAQKIILDCDPGHDDAIAMMLAWGNPNIDLLAVTTVVGNQTLEK
                     VSRNALAVAEIAHICNVPFAKGCARPLVREVENAPDIHGDSGMDGLVLPESTRQFEKK
                     HAAQLIIDLIMSHPEKSITLVPTGGLTNIALAARLEPRIIERVKEVVLMGGGYHTGNW
                     SAVAEFNIKIDPEAAHIVFNAGWKVTMVGLDLTHQALATPDVVERFKALNSKSGQFVV
                     ELLAFFAKMYKQAQGFDAPPVHDACAVAYVIDPSLIEVQQVPVNIELTGTHTLGMTVA
                     DFRYPPKQCNTWVAKKLDKSRFWDLVIESVKNLP"
     gene            complement(3872..5953)
                     /gene="recG"
                     /locus_tag="PWX02_001637"
     CDS             complement(3872..5953)
                     /gene="recG"
                     /locus_tag="PWX02_001637"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDL1729633.1"
                     /translation="MSEQLLDGVPLTALSGVGAAISEKLSRIGINNVQDLLFHLPMRY
                     EDRTRITPIADVRPESFATIEGYVQLTEVQFGKRPILSVTLSDSTSKIMLKFFNFNAG
                     MKNSLATGVRVKAFGEIKRGRFMAEIHHPEYQIIRDNQPLVLAETLTPIYSTTEGLKQ
                     ASLRKLTEQALALLEKVKVAELLPDEYNPHKYSLKEALQLLHRPPPTISAEVLEKGDH
                     PAQKRLIFEELLAHNLAMQQLRLGVKQQYAEALTYQTDIKQRFLDSLPFKPTHAQSRV
                     TADIEQDLAKPHPMMRLVQGDVGSGKTLVAALAALLAIDNGKQVALMAPTEILAEQHA
                     NNFANWLNPFGIEVGWLAGKVKGKARTAQLEAIKNGDVQMIIGTHALFQDNVEFHHLA
                     LVIIDEQHRFGVHQRLTLREKGAKENNYPHQLIMTATPIPRTLAMTVYADLDTSIIDE
                     LPPGRTPITTVVISEDRRAEIVQRVYQACKQEHRQAYWVCTLIDESEVLEAQAAAAIA
                     EDLQRALPDLRIGLVHGRLKPQEKQAIMAEFKAANLDLLVATTVIEVGVDVPNASLMI
                     IENSERLGLAQLHQLRGRVGRGATASHCVLMYKPPLGKISSKRLQVMRDSQDGFYIAE
                     KDLEIRGTGEILGTKQTGMAEFKVADLMRDRKMIPLVQRYAKQIISENQPLAEALIKR
                     WLDNKAEYTNA"
     gene            complement(5957..6385)
                     /locus_tag="PWX02_001638"
     CDS             complement(5957..6385)
                     /locus_tag="PWX02_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HDL1729634.1"
                     /translation="MYRYLFDTNIVSELYKLRNDKMDVNVRQWLRGVNPSQTNISCIT
                     LSEIKTGILLKARKDKEQSDRLNDWFENNVLKAYQTKAFVVNNDIALLASEYHIPNKM
                     DLNDAYIAATAKYHNLILVTRNTKDFIRCDVRLFNPFETA"
     gene            complement(6385..6672)
                     /locus_tag="PWX02_001639"
     CDS             complement(6385..6672)
                     /locus_tag="PWX02_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HDL1729635.1"
                     /translation="MAITITSREFNQNASKVLKMAEKEPVFITKWGKIVNVVSSYMDY
                     QQKKPSEPSFEELFASSENEDVSDEFWQEFEQTLVEIRKSNKPRPVDFGDE"
     gene            6836..8719
                     /gene="htpG"
                     /locus_tag="PWX02_001640"
     CDS             6836..8719
                     /gene="htpG"
                     /locus_tag="PWX02_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDL1729636.1"
                     /translation="MANNQETRGFQSEVKQLLQLMIHSLYSNKEIFLRELISNASDAA
                     DKLRFKALSEPALYEGDGELRVRVSVDETLGTITIADNGIGMTREQVIDHLGTIAKSG
                     TKEFLNALGTEQAKDSQLIGQFGVGFYSSFIVADKVTVKTRAAGVPAEQGVIWESAGE
                     GDYTVADLEKKERGTEIILHLREDEKEFLNEWRLREIIGKYSDHIGLAVEIQTNEYDD
                     EGKASGQKWEKINKAQALWTRSKNEISDEEYTEFYKHLSHDFSDPLIWSHNKVEGKQE
                     YTSLLYVPSKAPWDLFQREQKHGLKLYVQRVFIMDDAEVFMPNYLRFMCGLLDTNDLP
                     LNVSREILQENKVTQSLRSALTKRALQLLEKLAKDDAEKYAQFWQAFGVVLKEGVGED
                     FANKQQVAGLLRFASTQRDDSEQAVSFADYIARMKEGQKAIYFLTADSYQAAKNSPHL
                     ELFHKKGIEVLLLSDRIDEWLVSHLTEFDGKPLQSITKSDLDLGELADKQEEESQKAQ
                     QAEFGSFLERAKGYFGERVKNVVLTHRLTDTPAVVSTDSDEMTTQMAKLFAAMGQQAP
                     EVKYTFELNPDHPMVKKVADLADEAEFADWIELLFEQALLAERGTLENPTAFIKRVNK
                     LLG"
BASE COUNT         2435 a         1848 c         2031 g         2427 t
ORIGIN      
        1 atggctattt atgcaatatg gaataataaa ggtggggttg gtaaaagtta tttaactttt
       61 caaatcgctt gtgagtatgc aaaacaatac ccagcaaaaa aagttctcgt tgttgattta
      121 tgtcctcaag caaattcatc atctatgcta ttaggcggcc ttgaaaaagg tgagcaagag
      181 ttagcaaaaa ttcattctca agtaaacaag aatactattt caggatatat ttcagaaaga
      241 ttagtaagtc cttaccgttc tattaatagt acaacgaaat atgtaactca agttagcact
      301 ataaatgaat atgtaccaaa aaatgtttat cttgttgttg gtgatgagga attagaaatc
      361 caatcatctg gtattttaca tgcttcccaa gctgttcatg ttcctgattc ttggcggcta
      421 gtgcatcttt gggtgcgtga tttagtttct gatatacaga acgcttggga taatgatgat
      481 aacgttgtat ttattgattg caatccaagt ttcactattt atactgaatt agcgttggct
      541 gcttcagata gattgatcat ccctttttcc gctgatggtt catcaaaacg agctgttaag
      601 tctgtattat ctctagtata tggggttaaa aggcactctg gggatgtcag ctctcatttt
      661 tatacagaat ctcaaaagag taagttagca ttacctcaaa tttatatgta tgtaggtaat
      721 agattaacga taaatgccaa agcatcagca accgcattta aaacagttgt agaggaaata
      781 ggtgatgaaa tctataatat atggaaaact aatgctaatt tgttctgtat tcacctagca
      841 ggtgttggta ctcctacaaa taaatcaagc ttcagaaaaa tgtttcagta tgaggtgaga
      901 gatgcaaata cagcatctgt tgtttcaggt gctttaggta ttccgattag tcgcttatca
      961 tcaggaaaaa gagatgtttt aggtaaaaat gtaatggtga atcagtcaca attagatgct
     1021 caagttccta acattcaaaa tcttgttcag tgtatagaat aaagaaaaat agcgtatatt
     1081 aagtacgcta ttttttattt ctgacaaatc tttttatttg atagctcgat cacaaaaaag
     1141 aaatatctcc ttttaaataa aaggactttg ggctaatcta aacagagttt ttaaattaca
     1201 taggagtaaa aaatgactat tttccaagtt gcacaaaatt ggttagccca agacccggat
     1261 gcagagaccc gtgcggaatt agagcagtta attcaagcgg cagaatcgga cgagaaagca
     1321 aaagcagaat taacagcccg ttttgacgga cgtttacagt ttggtacggc gggtttacgt
     1381 ggtcgcttac aagcgggttc aatgggtatg aaccgtgttt tggtggcaca agctgccggc
     1441 ggtttagctg ggtttattaa aggttatgat aaagagccgt caattgttat tggttatgac
     1501 ggtcgtaaaa attccgatgt gtttgcccgt gacaccgctg aaattatggc agcggcaggc
     1561 attaaaacgt acctgcttcc tcgcaaattg ccaacaccag tgcttgccta tgcgattcaa
     1621 tattttgata ccacagccgg tgtgatggta actgccagcc acaacccacc ggaagataac
     1681 ggctataaag tttatttagg taaagctaac ggtggcggac aaattgtttc gccggcagat
     1741 aaagaaattg ctgctctgat tgataaagta gcagcaggcg atattcgtga tttacctcgt
     1801 agccaagatt tcacggtgtt agacgatgaa gtggtaaatg cttatattga gaaaaccgcc
     1861 tcactagcca aatggccaaa agccgaaata agctacgttt atactgcaat gcacggtgtg
     1921 ggctatgaag tgttaagcaa aacattagaa aaagccggct taccgcaacc gtatttagtt
     1981 agtgagcaga ttcagccgga cggttcgttc ccaacggtta atttcccgaa cccggaagag
     2041 aaaggggcgt tagatttagc gattaaattg gcgaaagaga aaaatgccga atttattatc
     2101 gccaacgacc cggatgctga ccgtttagcg gtagccgtgc ctgatgctca aggcaactgg
     2161 aaaccactgc atggcaatgt aatcggctgt ttcttaggct ggtatttagc caaacaattc
     2221 cacgcacaag gtaaacaagg cgtgttggct tgctcgttag tatcctctcc ggcgttagct
     2281 gaaattgcga aaaaatatgg tttaagctcg gaagaaacct taaccggctt taaatatatc
     2341 ggaaaagttg aaaacctatt atttggcttt gaagaagcct taggttattt ggttgaccca
     2401 gacaaagtgc gtgataaaga cggcatttcc gctgccatta tgttcttaga tttagtgtgc
     2461 agcctcaaac aagaaggtaa aacccttgcg gattacacca ccgagtttgt acaagagttc
     2521 ggggcttatg taagcggtca aatttcgatc agagtttgtg atttagccga aatcggcaaa
     2581 ttaatgacgg cgttacgcaa caatcctcca agcgaaattg gtggctttaa agtggctgag
     2641 tttattgacc ataccaaaac cacacgccaa aatgacattt tagtgtttgt gttggaaaat
     2701 ggcagccgtt tgattgctcg cccttcaggt actgagccga aaattaaatt ctatttagat
     2761 gctcgtggca cagacgctga aaacgcagag caagtgctgg ctcagtttga cgaaagtgtg
     2821 cgttcgctgc ttcgccaaga agaatacggc aaacaagatt gctaatggca ttaattgagg
     2881 gggagcttag gctctccctt ttttaaagga gacaataatg gctcaaaaaa ttattttaga
     2941 ttgtgatccg gggcatgacg atgcgattgc gatgatgctc gcttggggga atccgaatat
     3001 tgatttgttg gcagtgacta cggtggtagg caatcaaacc ttagaaaaag tatcgcgtaa
     3061 tgcgttggcg gttgcggaaa ttgctcatat ttgcaacgtt ccttttgcca aaggttgtgc
     3121 cagaccgctg gtgcgtgaag tggaaaatgc cccggatatt cacggtgatt cgggaatgga
     3181 tgggcttgta ctccctgaat ctacccgaca atttgagaaa aaacacgccg ctcaattaat
     3241 tattgatttg ataatgtcgc acccggagaa aagcattacc ttagtgccga ccggtgggct
     3301 aaccaatatt gctctagctg cccgccttga gccccgtatt attgaaagag tgaaagaagt
     3361 ggtgctgatg ggcggaggtt atcacaccgg caactggagt gcggtggcag aatttaatat
     3421 taaaattgac ccggaagctg ctcatattgt gtttaatgcc ggttggaagg tgacgatggt
     3481 cggcttagac ttaactcacc aagcacttgc gacccccgat gtggttgaac gctttaaggc
     3541 attaaacagc aaatcggggc agtttgtggt ggaattgctg gcgttcttcg ccaaaatgta
     3601 taaacaggct caaggctttg atgctccacc ggttcacgat gcctgtgcgg tggcgtatgt
     3661 gattgaccca agcctgattg aagttcagca agtgccggtc aatattgagc taacaggcac
     3721 gcatacactg ggtatgacgg tggcggattt ccgctacccg ccaaaacagt gtaatacttg
     3781 ggtagcaaaa aaattagaca aatcccgctt ttgggatttg gtaattgaat cggtgaaaaa
     3841 cctgccgtaa tctaaataac ccgcttgccg gttaagcgtt ggtatattcc gccttgttgt
     3901 ctaaccaacg cttaattaac gcctctgcaa gcggttgatt ttcgctaata atttgcttgg
     3961 cgtaacgttg caccagcgga atcattttgc ggtctcgcat taaatccgcc accttaaatt
     4021 ctgccattcc ggtctgtttt gtgccgagaa tttcacctgt gcctcgaatc tccaaatctt
     4081 tttcggcaat gtagaaaccg tcttggctat cacgcatcac ttgtaaccgc ttgctggaaa
     4141 ttttcccgag cggcggtttg tacattaaga cgcaatgcga ggcagttgcc cctcgcccaa
     4201 cacgtccacg caactggtgg agttgagcca gcccgagtcg ttcggaattt tcgataatca
     4261 ttaggctagc attcggcacg tccactccca cttcaattac ggtggtcgca accagcaaat
     4321 ctaaattcgc tgccttaaat tccgccataa tcgcttgttt ttcctgtggt ttcagccgtc
     4381 cgtgtactaa cccgatgcgt aaatccggca aggctcgctg taaatcttcg gcaatcgctg
     4441 ccgcggcttg agcctccaac acttcgcttt catcaatcag ggtgcagacc caataggctt
     4501 ggcggtgttc ttgcttacag gcttggtaca cccgttggac gatttctgct cgccgatctt
     4561 cagaaatcac cacagtcgtg attggcgttc tacccggtgg cagttcgtca atgattgagg
     4621 tgtcgaggtc agcatagacc gtcattgcca gcgttctcgg aatgggggtc gccgtcataa
     4681 tgagctgatg cggatagtta ttttctttcg ctcctttttc ccgcaaggtc agccgttggt
     4741 gtacgccaaa acggtgttgt tcatcaataa tcaccaacgc taaatggtga aattccacat
     4801 tgtcttggaa cagggcgtga gtgccgataa tcatctgcac atcaccgttt ttgattgcct
     4861 caagttgtgc ggttctcgcc ttgcctttca ctttaccggc aagccagcca acctcaatcc
     4921 caaagggatt aagccagttg gcaaaattat tggcgtgctg ttcggcaaga atttcggtcg
     4981 gtgccattaa tgccacctgc ttgccgttat caatcgctaa tagtgctgcc aaggcggcta
     5041 ctaaggtttt acctgaacca acatcgcctt gtaccagtcg catcatcggg tgtggtttgg
     5101 caagatcttg ctcaatatct gcggttactc ggctttgtgc gtgagtaggc ttaaaaggca
     5161 aactgtcgag aaaacgctgt tttatatcgg tttggtaagt aagtgcctct gcatattgct
     5221 gttttacccc taaacggagt tgctgcatcg ctaaattgtg tgccagcagt tcttcaaaaa
     5281 tcagccgttt ttgagcaggg tggtcacctt tttccaagac ttcggcagaa atcgtcggcg
     5341 gtgggcggtg caataactgc aacgcttctt ttaggctgta tttgtgcgga ttgtattcat
     5401 caggcaacag ctcggcgact ttcactttct ctaataaagc gagggcttgc tcggttaatt
     5461 tccgcagcga ggcttgtttt aacccctcag tggtggagta aatcggggtc agggtttcag
     5521 ccagtacaag cggttgatta tcccgaataa tttgatattc agggtggtga atttccgcca
     5581 taaatcgccc acgcttaatt tccccaaagg cttttacccg aacgccggtg gcaaggctat
     5641 ttttcattcc agcattgaaa ttgaagaatt taagcataat tttgctggtg ctgtcggaga
     5701 gtgtaaccga taaaatcggg cgtttaccaa actggacttc ggtaagttgt acatagcctt
     5761 caatggtggc aaagctttcc gggcgaacat cagcaatcgg ggtaatacgg gtgcgatctt
     5821 cataccgcat tggcagatga aacagtaaat cctgcacatt attaatgccg attcgactta
     5881 acttttcgga aattgccgct cctacgccgg aaagggcggt taatggcacg ccgtctaata
     5941 attgttcact cattatttat gcggtttcaa atgggttaaa taaacgcaca tcacaacgga
     6001 taaaatcttt ggtgttgcgg gtgactaaaa tcagattgtg gtatttggcg gttgccgcaa
     6061 tataagcatc atttaaatcc attttattgg gaatatggta ctctgatgcc aatagagcaa
     6121 tgtcgttatt caccacaaac gcttttgttt gataagcctt taatacatta ttttcaaacc
     6181 aatcatttaa tcggtctgat tgttctttgt ctttgcgggc tttcagcaaa atacctgttt
     6241 tgatttcaga taacgtaata cagctgatat tggtctggct aggattaacc cctctcagcc
     6301 actggcgaac attgacatcc atcttgtcat ttcttaactt ataaagctcg gaaactatat
     6361 tggtatcaaa aaggtagcga tacattactc atctccaaaa tcaacaggtc ttggtttatt
     6421 gcttttgcgg atttcaacca atgtttgttc aaactcttgc caaaattcat cgctcacatc
     6481 ctcattttcg gaagaggcga atagctcttc aaagcttggc tcgctaggct ttttctgctg
     6541 ataatccata taactagaca ccacattgac gattttgccc catttggtaa taaaaaccgg
     6601 ctctttttct gccattttta acactttgct ggcattttga ttaaattctc tggaagtaat
     6661 ggttattgcc atatcgatct ccaaaatgta gtaaaaagat gagtgtattt tattaaatgt
     6721 agtaatatct ttcaattaat tcgctatttg gcaacagata ttttgcaaaa aatcttgaaa
     6781 atctgaccac ttgccccatc tattccctgt tttttaatga ataaaggaat acacaatggc
     6841 aaataatcaa gaaacccgtg gcttccagtc ggaagtgaaa cagttattgc aactgatgat
     6901 ccactcgctt tactccaaca aagaaatttt cctgcgtgag cttatttcca acgcctctga
     6961 cgccgccgat aaattgcggt ttaaagccct ttccgagccg gctttgtatg aaggtgatgg
     7021 cgaactgcgt gtgcgtgtct ctgttgatga aacactcggt acgattacga ttgccgataa
     7081 cggtatcggt atgacccgtg agcaggtgat cgaccatctc ggtacgattg caaaatctgg
     7141 taccaaagaa tttttgaacg cactcggcac tgagcaagcc aaagacagcc agcttatcgg
     7201 gcagttcggc gtgggctttt actcttcttt catcgtggcg gataaagtga cagtcaaaac
     7261 ccgtgccgcc ggagtgcctg ccgagcaagg cgtgatttgg gaatctgccg gtgagggcga
     7321 ttacaccgtt gccgatctgg agaaaaaaga gcgtggcacg gaaatcattc ttcacttgcg
     7381 tgaggacgaa aaagagtttt taaacgaatg gcgtttgcgt gagattatcg gcaaatactc
     7441 cgaccatatc ggcctggcgg tggaaattca gaccaatgaa tacgatgacg agggcaaagc
     7501 aagcggtcaa aaatgggaaa aaattaacaa agctcaagcg ctttggacac gctcaaaaaa
     7561 tgagattagt gacgaggagt acaccgaatt ttacaaacac ctcagccacg atttttccga
     7621 cccgctgatt tggtcgcaca ataaagtcga aggtaagcaa gaatacacca gcttgctgta
     7681 tgtgccgagc aaagccccgt gggatctgtt ccaacgcgag caaaaacacg gcttgaaact
     7741 ctacgttcag cgtgtattta ttatggacga tgcggaagtc tttatgccga actacttgcg
     7801 ttttatgtgt ggcttgctcg acaccaacga cctgccgctc aacgtcagcc gtgaaattct
     7861 gcaagagaat aaagttaccc aatcgctccg ttctgcctta accaagcgtg cgttacaact
     7921 gctggaaaaa ttggcgaaag acgatgccga aaaatatgcc caattctggc aagcgttcgg
     7981 cgtggtgctg aaagagggcg tgggcgaaga ctttgccaac aaacaacagg ttgcaggctt
     8041 actccgtttt gcctccaccc agcgtgatga cagcgaacaa gcggtcagtt ttgccgatta
     8101 tattgcaaga atgaaagagg ggcagaaagc gatctacttc ttaaccgcag acagctacca
     8161 agcggctaaa aacagcccgc acttagagct gttccacaaa aaaggcattg aggtgctact
     8221 gctttccgac cgcattgatg aatggttggt tagccacctc accgagtttg acggcaaacc
     8281 gctacaaagc attaccaagt cggacttaga cttaggcgag cttgccgaca agcaggaaga
     8341 agagagccaa aaagcccaac aggcggagtt tggttcgttc ttagaacggg cgaaaggcta
     8401 ctttggtgaa cgggtcaaaa acgtggtgct gactcaccgc ttaaccgaca caccggcagt
     8461 ggtttccact gacagcgatg aaatgaccac ccaaatggca aaactgtttg cagcaatggg
     8521 gcaacaagca ccagaagtga aatatacttt tgagctgaac ccagatcacc caatggtgaa
     8581 aaaagtagct gatcttgccg atgaagccga gttcgccgat tggattgaac tgctgtttga
     8641 acaagctctg cttgctgaac gaggcacgtt ggaaaacccg acagccttta ttaaacgggt
     8701 aaataaattg ttgggctagc cattactaaa aacggaaatc t
//
LOCUS       DANJWC010000101         8710 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.101, whole genome shotgun sequence.
ACCESSION   DANJWC010000101 DANJWC010000000
VERSION     DANJWC010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8710
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.101"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            173..1396
                     /gene="hflK"
                     /locus_tag="PWX02_001641"
     CDS             173..1396
                     /gene="hflK"
                     /locus_tag="PWX02_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HDL1729637.1"
                     /translation="MSWNESGNQQDPWGKPGQKKAEPKPEQQEQGSQQEPANNQQNEQ
                     QPPDLEEVFSSLLKKMGGGKGSNNSSNQPTASLGKFLPVVLGLAAIVWAGSGFYTVQE
                     AERGVVTRLGKLDSIVMPGLNWKPTFIDSVTRVNVERVSELNTSGSMLTQDENMVQVE
                     MTVQYRVEDPAKYLFSVSNPDDSLKQATDSALRYVIGHMTMDEILTTGRATVRERTWS
                     TLRDIIKTYDMGLLVTDVNFQYARPPEEVKAAFDDAIKAQEDEQRLIREAEAYARGEE
                     PIARGQAQRTIEQAQAYKEAVVLNAKGEVERLSQLLPEYKASPELTRERLYIQTMEKV
                     MKNTPKVVMDSSGNNLNVLPFDKLMNSSSVIKAEQPAAAQVVTPQVVQPQVQPRTQIQ
                     PQQAEQQEPTRKGRF"
     gene            1399..2286
                     /gene="hflC"
                     /locus_tag="PWX02_001642"
     CDS             1399..2286
                     /gene="hflC"
                     /locus_tag="PWX02_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HDL1729638.1"
                     /translation="MRRLLVPILAVVAFVVLQAITIVNEGERGIMLRFNKVHRDSDQK
                     VVVYEPGIHFKVPFIDSLKVLDARIQTLDGQEDRFVTVEKKDLLVDSYVKWRISDFGQ
                     FYTSTGGDYQKAADLLRRKVGDRLRSEIGSRTIKDIVSGSRGELMAGAQKALNAGEDG
                     AERLGIEVVDVRVKQINLPNEVSSSIYQRMRAERDAVAREHRSQGNEKAEVIRAEVDK
                     KVVLILANANKTAQALRGEGDAQAAKLYSEKFGNEPEFYSFIRSLKAYEDSFAEGQNN
                     MMLLKPNSEFLRFMQAPTK"
     gene            2460..3758
                     /gene="purA"
                     /locus_tag="PWX02_001643"
     CDS             2460..3758
                     /gene="purA"
                     /locus_tag="PWX02_001643"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010869263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDL1729639.1"
                     /translation="MGKSVAILGAQWGDEGKGKIVDLLTDRVKYVVRYQGGHNAGHTL
                     IINGEKTVLRLIPSGILRDNVTCLIGNGVVLSPEALMKEMGELEERGINVRERLKISE
                     ACPLILPYHVAMDHAREAALGKNKIGTTGRGIGPAYEDKVARRGLRVSDLFDKEHFAA
                     KLKDILDYYNFQLVNYYKVEAVDYQKTLDDVFAIADVITGMVADVTTLLHEARENGDN
                     ILFEGAQGTMLDIDHGTYPFVTSSNTTAGGVATGSGYGPRHLDYVLGIIKAYCTRVGS
                     GPFTTELFDDVGAEIARKGNEFGAVTGRPRRCGWFDAVAVRRAVQINSISGFCMTKLD
                     VLDGFKELKICVAYKMPNGKIVEYAPLAAKDWEGVEPIYETMPGWSENTFRVTKREDL
                     PQAALDYIKRIEELVGVPVDILSTGPDRVETMILRDPFAA"
     gene            complement(3918..3994)
                     /locus_tag="PWX02_001644"
     tRNA            complement(3918..3994)
                     /locus_tag="PWX02_001644"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3958..3960),aa:Arg,seq:tct)
     gene            complement(4019..4095)
                     /locus_tag="PWX02_001645"
     tRNA            complement(4019..4095)
                     /locus_tag="PWX02_001645"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4059..4061),aa:Pro,seq:tgg)
     gene            4265..5125
                     /gene="folD"
                     /locus_tag="PWX02_001646"
     CDS             4265..5125
                     /gene="folD"
                     /locus_tag="PWX02_001646"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDL1729640.1"
                     /translation="MTAQIISGSEVSSQIKKKVSEKIAHYVEQGKRNPGLAVILVGND
                     PASQVYVGSKRKSCAEIGIESKSYDLPEETTEAELLALIEQLNQDDSVDGILVQLPLP
                     KQIDSTKVIEAILPHKDVDGFHPYNVGRLSQRIPTLRPCTPYGVMKLLETTGVDLYGQ
                     HAVIVGASNIVGRPMALELLLGGCTVTVTHRFTKDLEHHVRQADILVVAVGKPEFIPG
                     EWVKDGAIVIDVGINRGADGKLKGDVEYSVASQKAAFITPVPGGVGPMTVAMLMQNTL
                     QAYENHLQVV"
     gene            5240..6691
                     /gene="thiI"
                     /locus_tag="PWX02_001647"
     CDS             5240..6691
                     /gene="thiI"
                     /locus_tag="PWX02_001647"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDL1729641.1"
                     /translation="MKFIIKLFPEIMIKSDSVRKRFIKILTSNIRNVLLKETENVSVV
                     RHWDFIEVRAKVEEETALVLDLLKRTPGIHHILEVEETPFTDMHDIFEQTYAAVKAEL
                     EGKTFSVRVRRKGKHDFRSLDVERYVGGGLNQRIETAKVKLTNPDVTVRIDIEHDKML
                     LIKARHEGLGGYPIGTQEDVLSLISGGFDSGVSSYMLIRRGSRVHYCFFNLGGAAHEI
                     GVKQMAYHIWSRYSTSHKVRFIAINFENVVGEILEKVDNGQMGVVLKRMMVRAASRIA
                     ERFDIQAIVTGEALGQVSSQTLTNLRLIDKASDTLVLRPLITHDKEQIIAMAKAIGTD
                     DIAKSMPEFCGVISKNPTVKAIETKILEEEGNFNFEVLEQAVQTASYLDIREIAQQTE
                     KEVVSVETTTELSKNDVVLDIRSPEEVDEKPFRLDGVEVKELPFYKLSSQFSTLDQSK
                     TYLLYCQRGVMSKLQALYLKENGYSNVKVFRVK"
     gene            complement(6736..7443)
                     /locus_tag="PWX02_001648"
     CDS             complement(6736..7443)
                     /locus_tag="PWX02_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HDL1729642.1"
                     /translation="MFKEKPVISNQHGALAMAMLPFLYAVIESGFSTLHIFFGLSWLF
                     LYFFSYPFLSLFSKKPTERNKKWAKIYFGLSILFALPLIYAQPSILQFLPIILPLGLV
                     QVHYAKQKDERNLWNDIAGILTFGIMGMAAYYLVTKQYQFEILLHPTLFFIATTFYIK
                     SMVRERRNPIYMEISIAIHLVLAMLYILCSLQAVFFAYLIATARAIIVPSFGWNVKQV
                     GMLEFPVILLFLASLIW"
     gene            complement(7511..8485)
                     /gene="pfkA"
                     /locus_tag="PWX02_001649"
     CDS             complement(7511..8485)
                     /gene="pfkA"
                     /locus_tag="PWX02_001649"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDL1729643.1"
                     /translation="MSKKIKKIAVLTSGGDAPGMNAAIRGVVRTALNEGLEVCGIQDG
                     YYGLYHDKVIPLDRRSVSETINRGGTFLGSARFPDFKRPEVRKHCVETLKKYSIDALV
                     VIGGDGSYMGAKLLTEEFGYPCIGIPGTIDNDIVGTDYTIGYQTALETALDAIDRLRD
                     TSSSHQRISIVEIMGRHCGDLTLSAALAGGCEYVIVPEKGLDKDALMRSIEEGFHKGK
                     RHSIIAITELMTDVHALAKEIEARFGNETRATVLGHIQRGGSPCAFDRILASRMGVYA
                     VELLLQGFGGHCVGIQNEQLVHHDIIDAINNMRRPFKEELFEASRKLF"
BASE COUNT         2655 a         1586 c         2007 g         2462 t
ORIGIN      
        1 aattttaaga gcaatactag ggcgaattta tcgccctatt tttttgcaaa taaatagtga
       61 aaaaagaccg cttgtttcag ataaacctag tcaaaagctt actaaatagg tacaatcact
      121 aacttgcatt ctaagcttta aagtgcgatt cgattttatt ttggagatat ttatgtcgtg
      181 gaatgaatca ggcaatcagc aagatccttg gggtaaaccg gggcagaaaa aagctgagcc
      241 aaagcctgag caacaagaac agggttcaca acaagagccg gccaataatc aacagaatga
      301 acaacagccc cctgatttag aagaagtatt tagcagttta ttaaaaaaaa tgggtggtgg
      361 aaaagggtct aataatagtt caaatcaacc tactgcaagc ttagggaaat tcctacctgt
      421 tgttttaggt cttgctgcaa tcgtttgggc tggttccggt ttttatactg tacaagaggc
      481 tgagcgtgga gtagttactc gtttagggaa actcgatagt atcgtaatgc ctgggttgaa
      541 ctggaagccg acttttattg attctgtcac cagagtaaat gtggaacgtg tgtcagagct
      601 taatacaagc ggttcaatgc tgacacaaga tgaaaatatg gtgcaggtgg aaatgacggt
      661 tcaatatcgt gtggaagatc cggctaaata tttgtttagt gtaagcaatc ctgatgacag
      721 cttaaaacaa gctacagata gtgcattgcg ttatgtgatt ggtcatatga cgatggacga
      781 aattttaact actgggcgtg caaccgtacg cgaaagaacg tggtccacat tgcgtgatat
      841 tatcaaaact tatgatatgg ggttattggt aacagacgta aacttccaat atgcacgacc
      901 gcctgaagag gtgaaagctg cctttgatga tgcaatcaaa gcacaagaag atgagcaacg
      961 cttaattcgt gaagcggaag cctatgcaag aggtgaagag ccgattgcac gtggccaggc
     1021 acagcgtacg attgagcaag ctcaagctta caaagaagca gttgtgttaa atgcgaaagg
     1081 ggaagtggag cgtttgagtc aattattacc tgaatataaa gcctctccag agctgactcg
     1141 tgaacgttta tatatccaga caatggaaaa agtcatgaaa aatacgccga aagtggtgat
     1201 ggattcaagt ggtaataatt tgaatgtatt gcctttcgat aagctaatga attcttcatc
     1261 ggtaataaaa gcggagcagc ctgcggcggc acaagtagtt acaccacaag ttgttcagcc
     1321 gcaagtacag ccaagaacac aaattcagcc gcaacaagca gaacaacaag aaccaactcg
     1381 taaggggaga ttttaatcat gcgtagatta ttagtaccta tacttgctgt agttgctttt
     1441 gttgtgctac aggcaatcac cattgtaaac gagggcgagc gtggcattat gttacgtttt
     1501 aacaaagttc atcgtgatag cgatcaaaaa gtggtggtat atgaaccggg gattcacttt
     1561 aaagtaccat ttatcgatag tttaaaggta ttagatgctc gtattcaaac tctagatggg
     1621 caagaagacc gttttgtaac agtcgagaaa aaagatttat tagtggactc ctatgtaaaa
     1681 tggcgaatca gtgattttgg ccaattttat acctctactg ggggggatta ccaaaaagcg
     1741 gcggatttat tacgccgtaa ggtaggggat cgtctgcgtt ccgagattgg ttctcgaacc
     1801 attaaagata ttgtatccgg ctctcgtggt gagttaatgg caggagccca aaaagcgctg
     1861 aatgccggtg aagatggagc agagcgttta ggaattgaag tagttgatgt gcgagtgaag
     1921 caaatcaact taccaaatga agtttcttct tcaatttacc aacgtatgcg tgccgagcgt
     1981 gatgcggtgg ctcgtgaaca ccgctcacaa ggtaatgaga aagcagaggt aattcgtgcc
     2041 gaagtggata aaaaagtggt gctgattttg gcaaatgcga acaaaactgc tcaagccttg
     2101 cgaggtgaag gtgatgctca agctgcaaaa ctctattcag agaaatttgg taacgagccg
     2161 gaattttaca gttttattcg tagtttaaaa gcctatgaag atagctttgc cgaaggtcaa
     2221 aataatatga tgttattaaa gccaaacagc gaattcttac gctttatgca agcaccgaca
     2281 aaataattgt atctataaaa cccgatcttg tatcgggttt ttaattttcg tccctattct
     2341 gatcatagca agcggtcaat tttggctgat tttttgcaaa aaatttgaaa aaattgaccg
     2401 cttgcgatat gaagttataa tgggtttcgt tattcatcct aactaaaaga ggcatttcta
     2461 tgggtaaaag tgttgctatt cttggcgctc agtggggcga tgagggaaaa ggcaaaattg
     2521 ttgatttatt aacagatcgt gtgaaatatg tagtacgtta tcaaggtgga cataatgcag
     2581 ggcatacctt aatcattaat ggtgaaaaaa ccgttctgcg tttaattcca tcgggtattt
     2641 tgcgtgataa cgtgacttgt ttaatcggta atggtgtagt gctttctcca gaggcattaa
     2701 tgaaagagat gggggagcta gaagaacgag gcattaatgt gcgtgagcgt ttaaaaattt
     2761 ctgaagcttg cccgttaatt ctgccttatc acgttgcgat ggatcacgct cgagaagcgg
     2821 cacttggtaa aaacaaaatc ggtacaacag gacgtggtat tggcccagct tatgaagata
     2881 aagtcgctcg ccgtggttta cgtgtcagcg atttattcga caaagaacat tttgccgcaa
     2941 aattaaaaga tattttagat tactacaact tccaattagt caattattac aaagttgaag
     3001 cggtagatta tcaaaaaacc ttagatgatg tgtttgcgat tgcggatgtg attaccggaa
     3061 tggttgccga tgtgacaact ttacttcacg aagcacgtga aaacggcgac aatattttat
     3121 ttgaaggggc tcaaggcacg atgttagata tcgaccacgg tacttacccg ttcgtaacca
     3181 gctcgaatac gacagcaggc ggtgtggcaa ccggatcagg ctacggtcct cgtcatttag
     3241 attatgtatt agggattatc aaagcctact gtacccgtgt aggtagtgga ccgttcacca
     3301 ccgaactatt tgacgatgta ggtgctgaaa ttgctcgcaa aggtaatgag ttcggagcgg
     3361 taaccggtcg ccctcgccgt tgtggctggt ttgatgccgt agccgttcgc cgagcagtgc
     3421 aaatcaactc catttcaggc ttctgtatga ctaaactcga tgttttagac ggattcaaag
     3481 agctaaaaat ctgcgttgcc tacaaaatgc caaacggaaa aattgtcgaa tatgcccctc
     3541 ttgcagcaaa agactgggaa ggtgttgaac caatttatga aacaatgccg ggttggtctg
     3601 aaaatacttt ccgtgtgaca aaacgtgaag atttaccaca agccgcatta gattacatta
     3661 aacgtatcga agagctggtc ggtgtgccgg tagatattct ttctacaggt ccggatcgtg
     3721 tcgaaacgat gattttgcgt gatccttttg cagcttaata aaagataaaa taaagccatt
     3781 tagtgagata gagctagatg gcttttttgt ttgaaccagt aaactttact ttggcacaat
     3841 tttgtcacaa tacagaaaat agttctttag cgtgagtttt taaggctgtg taagtatttg
     3901 aatttaaaag aatttaatgg cacgccctaa aggattcgaa cctttgaccc acgccttaga
     3961 agggcgttgc tctatccagc tgagctaagg gcgcagatga agataatata aaaagaagtg
     4021 gtcggcgaga taggatttga acctacgacc cactggtccc aaaccagttg cgctaccaag
     4081 ctgcgctact cgccgacata agatgttgtt gattatagat ttctttgcac aacagtcaat
     4141 ctttttttgt gaatgtcggc ttaaatgatt gaagtttatc cgttattcaa aattagctta
     4201 tcaaaaaggc tattttttga gaaaatgacg aatgttttgc caagtgatat ttcgggaggt
     4261 agtaatgact gcacaaatta tttctgggag tgaggtttct tcacaaatta aaaaaaaagt
     4321 gagtgagaaa atcgctcatt atgttgaaca gggtaaacgt aaccccggtc ttgcggtcat
     4381 tttagtgggg aatgacccag cctctcaagt gtatgtgggg agtaaacgca aaagttgtgc
     4441 tgaaattggg attgagtcga aatcttatga tttacctgaa gaaactacag aggctgaatt
     4501 attagctctg attgaacagc tgaatcaaga tgattcggtc gatgggattt tagttcaatt
     4561 accgttacct aaacaaattg attcgactaa agtgattgaa gccatcttac cacataaaga
     4621 tgttgatgga tttcatcctt ataatgttgg gcgtttatct cagcgtattc ccactttacg
     4681 cccttgcacg ccatacggtg tgatgaagtt gcttgaaaca acaggggtag atttatacgg
     4741 tcaacacgca gtgattgtag gtgcgtctaa tattgttggt cgcccgatgg cgttagaatt
     4801 gttgcttggt ggttgtacag tcactgtcac tcatcgcttt accaaagatt tagagcatca
     4861 cgttcgtcaa gcagatattt tagttgtagc agtcggcaag cctgaattta ttcccgggga
     4921 gtgggttaaa gacggtgcta tcgtgattga tgtagggatt aaccgtggag cagacggcaa
     4981 actaaaaggt gatgtagagt attcagttgc ctcgcaaaaa gcggcattta ttacccctgt
     5041 tcccggtggt gtaggcccga tgacggtggc aatgttaatg caaaatacct tgcaggcgta
     5101 tgaaaaccat ttacaagtgg tctaatttga cagatatttt gcaaatcctc ggcattggtc
     5161 gaggatttct ttttctaggc tttttcagca gaaaaggcta aaatactcgc catttttgtc
     5221 acactattct gatttaaata tgaaatttat catcaaactt ttccctgaaa ttatgattaa
     5281 aagcgattct gttcggaaac gctttatcaa aattttaacc tcaaatattc gtaacgtatt
     5341 attaaaagag actgaaaatg tgtctgtggt tcgccattgg gactttattg aagttcgggc
     5401 gaaagtggag gaagaaacgg cattagtgct ggatttactt aaacgcacgc cgggtattca
     5461 ccatatttta gaagtggagg aaaccccttt tacggatatg cacgatattt ttgagcagac
     5521 ttatgccgca gtgaaagctg aactagaagg caaaacgttt agcgtgcggg ttcgccgtaa
     5581 aggaaagcac gatttccgtt ctcttgatgt agagcgttat gtcggtggtg ggctgaatca
     5641 gcggattgaa acggcgaaag tgaaattaac caacccagat gttacggtca gaattgatat
     5701 tgaacatgat aaaatgttgc tgattaaagc tcgccacgaa gggttaggcg ggtatccaat
     5761 cggcacacaa gaagatgtgt tgtcattgat ttcgggtggt tttgattcgg gtgtatcgag
     5821 ctatatgctg attcgcagag gttctcgtgt gcattactgt tttttcaatt taggtggtgc
     5881 cgcacacgaa attggcgtga aacagatggc atatcatatt tggagccgtt atagtacctc
     5941 gcacaaagtc cgttttatcg ccattaattt tgaaaatgta gtgggtgaga ttttagaaaa
     6001 agttgataac ggacaaatgg gcgtggtatt aaaacgtatg atggtgcgag cggcaagccg
     6061 tattgccgag cgttttgata ttcaagcgat tgtgaccggt gaggcgttgg ggcaggtctc
     6121 tagccaaaca ctgactaatt tacgtttaat tgataaggca tcagatacgc tggtattacg
     6181 tccgctcatc actcacgata aagagcagat tattgcaatg gcaaaagcga taggcactga
     6241 cgatattgct aaatcaatgc cggagttctg tggagtaatt tctaaaaatc cgacggttaa
     6301 ggcaatcgaa actaaaattt tggaagaaga aggcaacttt aattttgagg tactagaaca
     6361 agcggttcaa acggcgagtt atttagatat tcgtgagatc gctcagcaaa ccgaaaaaga
     6421 ggttgtttcg gttgaaacca caacagaatt gagtaaaaac gatgttgtgc tagatattcg
     6481 cagcccggaa gaggttgatg aaaaaccatt ccgtttagac ggggttgaag taaaagaact
     6541 cccgttttat aaactttcca gccagtttag tacgcttgac caaagcaaaa cctacttgct
     6601 ctattgccaa cgtggggtaa tgagtaaact gcaggctctt tatttgaaag agaatggcta
     6661 cagcaatgta aaagtgtttc gagtgaaata atgcctcttt gattgcacct taatgattga
     6721 ttaaggtgca attttttacc aaataaggct tgctaaaaag agtaagatca caggaaattc
     6781 aagcattcca acttgtttaa cattccagcc aaagcttggc acaataatgg ctcgggcagt
     6841 tgcaattaaa taagcaaaaa agaccgcttg tagagagcag agtatgtaaa gcattgctaa
     6901 aaccaaatgt attgcaatac tgatttccat ataaattggg tttcttcgct ctcgcaccat
     6961 actctttata tagaaagtcg tagcaataaa aaatagggta gggtggagca gaatctcaaa
     7021 ctgatattgt ttcgttacca gataataagc tgccattccc attatgccaa aggttagaat
     7081 gccggcaata tcattccata aattgcgttc atctttttgt ttggcgtagt gaacttgcac
     7141 taacccaagc ggtagaatta tcggtaaaaa ttgcaaaatc gaaggctgag cgtagatcaa
     7201 aggtaatgca aatagaatgc ttaagccaaa gtagattttc gcccattttt tattgcgttc
     7261 agttggcttt ttactgaaaa gtgagagaaa agggtaggaa aagaagtaga gaaacagcca
     7321 cgacagccca aagaaaatat gcaaagtgct aaaaccactt tcaataacgg cgtagagaaa
     7381 cggcaacatt gccattgcta atgcaccgtg ttggttggat ataacgggtt tttctttgaa
     7441 catttaattt ccctcttaat gtggcaaata aaaaggcgac taaacagccg cctttaaatt
     7501 gtaagaaaag ttagaataat tttctagagg cttcaaataa ttcttctttg aacggacgac
     7561 gcatattatt gatcgcatca ataatatcgt ggtgaaccaa ctgctcgttt tgaataccga
     7621 cacagtgacc gccaaagcct tgcagtaata attccaccgc atagacgccc atacgggagg
     7681 ccaaaatacg gtcgaaggca caaggcgaac caccacgttg aatatgacct aatacggttg
     7741 cacgggtttc attgccgaaa cgtgcttcaa tttctttcgc taatgcgtga acatcggtca
     7801 tcaattctgt aattgcaata attgaatggc gtttaccttt gtggaaacct tcttcaatgc
     7861 ttcgcattaa tgcatcttta tctaagcctt tttccggtac gatcacatat tcgcaaccac
     7921 cggcgagggc agcacttagg gttaaatcac cacagtggcg acccataatt tccacaatcg
     7981 aaatacgttg gtgtgaactt gaagtatcac gcaggcggtc aatcgcatct aatgcggttt
     8041 caagggcagt ttgatagccg atggtgtaat ccgttcccac aatatcgtta tcaatcgtac
     8101 ccggaatacc gatacaaggg taaccaaatt cttcggttaa taatttagcc cccatataag
     8161 aaccgtcgcc accaataacc actaatgcat caatgctgta tttttttaag gtttcgacac
     8221 aatgtttgcg aacttctggt cttttaaaat cagggaagcg ggcagaacct aagaatgtac
     8281 cgcctcggtt gatggtttca gacacggaac ggcggtcaag tgggatcact ttatcgtggt
     8341 aaagcccata gtagccgtct tgaataccac aaacctctaa accttcatta agtgcggtac
     8401 gaaccacacc acggattgcc gcgttcattc ctggtgcatc accaccactg gttaatactg
     8461 caattttttt gattttttta ctcatttaaa aacaccttgt tgataaatta aagttaaatt
     8521 ggaaactggt gcaaggttac aatattcagc cttttcgttc tactaaaaaa tacgtatttt
     8581 tttgatttag cttagctttc ttgcaaaatt tcaagggtaa aatctagagc gttgaaaaaa
     8641 tgctcaacat cttgttgatt attgtaatgg gcaagtgaaa gtcgaactgt tccgacctct
     8701 tctaaatggc
//
LOCUS       DANJWC010000102         8647 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.102, whole genome shotgun sequence.
ACCESSION   DANJWC010000102 DANJWC010000000
VERSION     DANJWC010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8647
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.102"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..228
                     /locus_tag="PWX02_001650"
     CDS             <1..228
                     /locus_tag="PWX02_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005714533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1729644.1"
                     /translation="FIDTTINYYNNEGVYIFVIDDEVYIKRLQKIPGGIYRALSDNKT
                     YEPFDIKRETLDTAIILGKFIRVLPINPKDL"
     gene            303..683
                     /locus_tag="PWX02_001651"
     CDS             303..683
                     /locus_tag="PWX02_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729645.1"
                     /translation="MNNKRIKPEQFADFITQRTGRNLTCPICNSQEHYLHDGYDTFTE
                     LCGEKIIGVPTIPYRIEPSPDEVMKFAAPEFHSLHFNEREGTKYWLDQKARTAVIVTC
                     VCCNNMLFFDRERILEWIKGSQNE"
     gene            688..1173
                     /locus_tag="PWX02_001652"
     CDS             688..1173
                     /locus_tag="PWX02_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729646.1"
                     /translation="MIQNDPRDAIILSLLANAVYGGHNNSQISYQETEEVNLDARLRQ
                     VEGDVREIKSNYLTKEAFYKAGGSTLIALVVTAGIALWTVYSHLDTKVEARFSKIDDK
                     FVQIETNIKNLDIRLTKVESRLDNVEQRLDNVEQRLGNMEKKIDGVDNKLDLLLQQFK
                     R"
     gene            1304..1486
                     /locus_tag="PWX02_001653"
     CDS             1304..1486
                     /locus_tag="PWX02_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDL1729647.1"
                     /translation="MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKE
                     IGTGLVNKIKKDLDLK"
     gene            1525..1944
                     /locus_tag="PWX02_001654"
     CDS             1525..1944
                     /locus_tag="PWX02_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012621799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL1729648.1"
                     /translation="MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGL
                     ATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMKVFLLNEMIKQGVRKAEMARRLD
                     VHLPQIDRLLDFNHSTKVEFVEKAYGKLNQRFTILPH"
     gene            complement(2011..2316)
                     /locus_tag="PWX02_001655"
     CDS             complement(2011..2316)
                     /locus_tag="PWX02_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDL1729649.1"
                     /translation="MRKPAHPGQVLLDGFIEPNNIKIKELADHLGFSRETLSRVLHGK
                     TPMTANLALSLEEAGISTAKLWLNLQTKYDLWELKQQRLNPVMPFHFGGNYGETAHA"
     gene            complement(2325..2600)
                     /locus_tag="PWX02_001656"
     CDS             complement(2325..2600)
                     /locus_tag="PWX02_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011039853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL1729650.1"
                     /translation="MIKSFKHKGLKQYFEKGITKGIQLNHQRKIDGILDLIDSAESID
                     EFMPFYQCHELKGNRKGIYSMTVNGNWRITFEFVNGDAYILNYEDYH"
     gene            2890..3120
                     /locus_tag="PWX02_001657"
     CDS             2890..3120
                     /locus_tag="PWX02_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729651.1"
                     /translation="MNAQTTLNNHKDYILCGRKEKRTSDFINVFEVFENEATQEFVIE
                     RAMFRNGKLIDWNQSDKMNAEQAQQLWQAYIH"
     gene            complement(3599..3922)
                     /locus_tag="PWX02_001658"
     CDS             complement(3599..3922)
                     /locus_tag="PWX02_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005715000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729652.1"
                     /translation="MWEVLIPSIFSIIKEHFDNIVMRITTWFLSFIICWLYIPIDIQD
                     HLLANALPGLPTYTLLYIFYLVAATAFWQMFILCLDVFALIINKIIKSPAMDQQTNRV
                     DVDNK"
     gene            4115..4300
                     /locus_tag="PWX02_001659"
     CDS             4115..4300
                     /locus_tag="PWX02_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729653.1"
                     /translation="MNKMKQLAPFALLILLLGIVGRMDYDDHIQMQRYKCERNQGVWQ
                     VESNGNQYCGGKYDTKY"
     gene            4284..4448
                     /locus_tag="PWX02_001660"
     CDS             4284..4448
                     /locus_tag="PWX02_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729654.1"
                     /translation="MTRNIETGVEIVAVGKLQHSTYPQYSRQNAIMLINRKIERFNVG
                     RDKPISLYKE"
     gene            4454..4912
                     /gene="ssb"
                     /locus_tag="PWX02_001661"
     CDS             4454..4912
                     /gene="ssb"
                     /locus_tag="PWX02_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL1729655.1"
                     /translation="MAGINKVIIVGRLGNDPELRTMPNGDSVAKISVATSTEWTDKVS
                     GDKKQATEWHSIIAFRNLADIIGKYLKKGSQVYVEGKLRTRKWQAQDGTDRWTTEIIA
                     DQLQMLGSSNSGNSNNNWATEPAGNPPPTNPYNHVMTDSESRDFDDDIPF"
     gene            4948..5307
                     /locus_tag="PWX02_001662"
     CDS             4948..5307
                     /locus_tag="PWX02_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729656.1"
                     /translation="MAKTNVPEFLDELDCGIFKDKLATALSDVALGVLTHDKKGKVTV
                     EFSLDKMDSDSPSVQIQHKLSYVKPTKRGKSAEEDTTATPMYVHKGGALSATPEKTET
                     AVKESPSGLKALSKAAA"
     gene            5379..6182
                     /locus_tag="PWX02_001663"
     CDS             5379..6182
                     /locus_tag="PWX02_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HDL1729657.1"
                     /translation="MDKTTLQQISALAVAATKELETDFGAVMLPEGIKLQNLEPFQAH
                     RNQFRAAFSTQRFGSLIEYATANAQENAQCFIDQEKMSAEIVFDMGNREQAGHAKHRA
                     NLVMKKTAAYKALCEINGSRRSQRDFSDFLEDWGIYLNAYHHEDEISIKNAVQAVRKM
                     TIDHARNEEHELSDFAAKKSAMESVEAKSTLQLPTHLVFTCNPYNGLDTRSFTLRVQV
                     LTGSGEPVLTARLVHAEQIEESIATEFAEKLADALSETSIKVNIGTIAI"
     gene            6276..6710
                     /locus_tag="PWX02_001664"
     CDS             6276..6710
                     /locus_tag="PWX02_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729658.1"
                     /translation="MKTTQHILDEREQQHGNYNSFAKIYGGLRKVSDPHAEKLTWRQQ
                     ISVEMILFKLARILNNGSNHQDSWQDIAGYALLGGDIYTPQSSDNTNTKGLPKPLTDS
                     IYPESHLDKNAVWRLDLEFETKEQAVAVLEAVTGKKYSENIT"
     gene            6720..7208
                     /locus_tag="PWX02_001665"
     CDS             6720..7208
                     /locus_tag="PWX02_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HDL1729659.1"
                     /translation="MSKEKYFSVDVSNDIHILNLHETLEQAKQSCLNGAAEAYEFASE
                     MDDCENYEANDLPYAVYGVVLGRAKSDIRPLTSEEKESGYFEEVDNFIEQPELVETNG
                     WISVKDKLPEIHEEVLVTNNFIMIAKFNGESWITSGGILGIQPVYDVTHWQPLPEPPR
                     EQ"
     gene            7229..7438
                     /locus_tag="PWX02_001666"
     CDS             7229..7438
                     /locus_tag="PWX02_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729660.1"
                     /translation="MFRNELQVMDGKRYVVLECQFRREWKVAIESRGTVTSGEAIEIC
                     QYWIKYKGVKPEQLKVVEVPDILKE"
     gene            7585..7710
                     /locus_tag="PWX02_001667"
     CDS             7585..7710
                     /locus_tag="PWX02_001667"
                     /inference="COORDINATES: protein motif:HMM:NF021626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1729661.1"
                     /translation="MQSLRLIEIRCKYCQKLLAKAKDVQHLEIKCVRCKSINKFN"
     gene            7757..8599
                     /locus_tag="PWX02_001668"
     CDS             7757..8599
                     /locus_tag="PWX02_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729662.1"
                     /translation="MAKQIFKQAPLPFVGQKRMFLAQVSQILNENIPDDGQGWTIIDV
                     FGGSGLLAHTVKHIKPKAHIIYNDYDGYAERLKHIPDTNRLRKQIYDIIGESTPKNKR
                     LDPDKKSQVINVIQSFDGYIDVNCVASWLLFSGQQINSLENLFNKIFWNGIRQTDYPS
                     AEGYLDGIEVTHESFHKLLPRFQHKDKVLLLLDPPYLCTRQESYKQATYFDLIDFLRL
                     INLTKVPYIFFSSTKSEFVRFVDFMVSEKKDNWQTFENAKWIKVKASLNYQSTYEDNL
                     VYKF"
BASE COUNT         2984 a         1588 c         1704 g         2371 t
ORIGIN      
        1 tttattgata ctacaatcaa ttattacaat aacgagggtg tttatatttt tgttattgat
       61 gatgaagttt atataaaaag actacaaaaa attccaggtg gtatttatag agccctatcg
      121 gacaacaaga cttacgaacc ttttgatata aaaagagaaa ccttagatac tgctattatc
      181 cttgggaaat ttattcgagt attgccaata aatcctaaag acttgtaggc attagcgtag
      241 cggttgagca tcggagctat ttgtagccag cggttgtttt attgggtaga aaggaagtta
      301 aaatgaataa taaacgcatt aaacctgaac agtttgcgga ttttatcacg caacgtactg
      361 ggcggaattt aacttgccct atttgtaata gccaagaaca ttatttacac gatggctatg
      421 acacttttac ggaattatgc ggagagaaaa ttatcggtgt tccaaccatt ccataccgaa
      481 tagagccatc tcctgatgaa gttatgaaat ttgcagctcc tgaatttcat tctttgcatt
      541 tcaatgaacg ggaaggaaca aaatattggt tagaccaaaa agcgagaaca gccgttattg
      601 tcacttgtgt ttgctgtaat aatatgcttt ttttcgacag agagagaatt ttagaatgga
      661 ttaagggctc acaaaatgaa tagaccaatg attcaaaatg atcctagaga tgcaatcata
      721 ctatccttac ttgcaaatgc agtatacggc gggcataata attcacaaat tagttatcag
      781 gagactgaag aagtgaattt agatgctcga ttaagacaag ttgaaggcga tgtaagagaa
      841 atcaagtcaa attatttgac gaaagaggct ttttataaag ctggtggctc tacactcatt
      901 gcattagtag ttactgctgg aattgcatta tggacagtat attcacacct tgacaccaaa
      961 gttgaagctc gattctcgaa aattgatgat aagtttgtcc aaatagagac caatatcaaa
     1021 aatcttgata ttcgcctcac taaagttgaa tctagattag ataatgtcga acaacgacta
     1081 gacaatgttg agcagcgact aggcaatatg gagaagaaaa tagatggggt tgataataaa
     1141 ctcgatctgt tactacaaca attcaaaaga taaaataata ttccttgaat aaaccgccct
     1201 cgtggcggtt ttcttttctg ctacaaaaaa acacaacaaa aatgttgcaa taccaacaaa
     1261 aatgttgtat tatttccttg ctgttttgat aaggaggtat cttatgaaat acagcgagtt
     1321 cctgaggtac ttacttgctc aaggttgcga aattgaaaac cataggcgag gcagtcatcg
     1381 aaaggtaaca ctaaacggaa aacaatcggt ttttccttat cacgggagca aagagattgg
     1441 tacaggttta gtaaataaga ttaagaaaga cttagatctt aaataacaaa gtccctcgaa
     1501 agaggggctt ttctaggagt ttatatgtta cgctacccag ttgaaattac ccctgatgat
     1561 aacggcacat accttgtgac ttgccctgat attcctgaaa tggcaagcgt aggtgaggac
     1621 ttagaagaag ccctattaga agcacaagat ggcttagcca cagcccttga attctatttt
     1681 gatgatagac gagaaatacc aatgccaagc ccaattaaag agggacaaca tacggttaat
     1741 cttaccgtgc tgcaatcaat gaaagtattt ctcttaaatg aaatgattaa acaaggtgta
     1801 cgtaaagcag aaatggcaag acgattagat gtacacttac cacagataga tcgcctatta
     1861 gattttaatc attccacaaa agtagaattt gttgagaaag cctatggtaa gctaaatcaa
     1921 cgctttacta ttctccctca ctaataaaat aaccgcctac gggcggtttt cttttcctaa
     1981 gaacaataaa accctccgaa gagggcatta ttaagcgtga gcagtttctc cataatttcc
     2041 accaaaatga aaaggcataa caggatttaa ccgctgctgc tttaactccc ataaatcata
     2101 tttggtttgt aagttgagcc ataacttagc cgtactaatt cctgcttctt ctaaacttaa
     2161 cgccaaattt gcagtcattg gtgttttgcc gtgtaatact cttgataaag tttcacgaga
     2221 aaacccaaga tgatctgcta attctttaat tttgatgtta tttggttcaa taaacccatc
     2281 taataaaact tgacctggat gtgctggttt acgcataact ccccctaatg ataatcttca
     2341 taattcaaaa tataagcatc gccatttaca aattcaaatg taatccgcca gttgccatta
     2401 accgtcatag aataaatgcc ttttcgattt ccttttaact cgtggcattg gtaaaaaggc
     2461 ataaattcat caatactttc tgctgaatca atcaaatcta aaataccatc gattttacgc
     2521 tgatgattaa gctgaatacc ttttgttatc cctttttcaa aatattgttt taagccttta
     2581 tgcttgaaac tcttaatcat aatgataccc gccaaacaaa tgtgatataa atatatcaca
     2641 aaaaataagt aaggcaagat atttcgagtg tattcctgtg taattatcct ttctctctat
     2701 gcattctgtt taaaaaacaa gcaatcaaac aacctttcta aaaataaatt ctctttaaaa
     2761 tcaataaatt actaaacaaa agctaaactt tatttagttt acactaaata aacactttac
     2821 taaatgttta atatttacta aactacaccc atcaaaacaa acaacgccac agacaaaggg
     2881 gaaaccaaaa tgaacgcaca aacaacgcta aacaaccaca aagactacat tttatgcgga
     2941 cgcaaagaaa aacgcaccag tgatttcatc aatgtgtttg aggtctttga aaatgaagcc
     3001 acgcaagagt ttgtgattga aagagcaatg ttcagaaatg gcaagttaat cgactggaac
     3061 caaagcgaca aaatgaacgc tgagcaagct caacaacttt ggcaagccta cattcactaa
     3121 gaatttttat caaagccctt tactgagggc ttgaataaag gttctagacc tagcccacat
     3181 agcaggcaat agccgagaga taagcagaga ctgtgggatg ttctttaaca atttagtgct
     3241 tgtgcggacg atattaacaa cctcgagcag ttgtaaagta atgctttatc actcggcaag
     3301 gttggttaga ccctgacata caaatttgaa cgacttgcca aacagaggtg gcaaggttta
     3361 ggcaacacac tcgcaaggtg tggagagctg aaaaggcaac actggtatca gtcctaagca
     3421 atcacgctta tctcaagttt gagtaagtac ggatacaagc agcacccgac ggaatggcaa
     3481 aagccggacg ggcgacggcg tgggcagaca cgcattattc caaagcgtat ttaagaaagt
     3541 atgttctgga atatttaact agaggtaaat tatgaaggta aaacaatgct tattaatttt
     3601 atttattatc aacgtcaacc ctattggttt gttgatccat agctgggctt ttaataattt
     3661 tattaattat caatgcaaag acatccaagc aaaggataaa catttgccaa aaagctgtag
     3721 cagcgaccaa ataaaaaata tagagcaatg tataagtagg tagtcctggt agggcattag
     3781 ccagtaaatg atcttgtata tctattggta tatatagcca acagataata aaggacagaa
     3841 accaagttgt aatacgcata actatgttat caaagtgttc ttttattata gaaaagatag
     3901 agggtatcaa tacttcccac attgtaaagt tcctgatttt tagtgatgag aggagaggtc
     3961 tttaattata ttcctgcgag tggtgagaga caagcagggc ttgctgtccg tggcaagtat
     4021 aaaatcacgg acactgttta ctacaatcta gacgtaagtc gctccatttg cccaccgtaa
     4081 aacgtgggct ttttttaccc gaaaggacaa tcctatgaac aaaatgaaac aacttgcccc
     4141 atttgccttg ctaattttgc tattaggcat tgtcgggcga atggattatg acgaccacat
     4201 acaaatgcaa cgttacaaat gcgaacgaaa tcaaggcgtt tggcaagtgg aaagcaacgg
     4261 caatcaatac tgcggaggca aatatgacac gaaatattga aacaggcgta gagattgtgg
     4321 cggtcggcaa attgcagcat tcaacctatc cacaatacag ccgccaaaac gcaattatgt
     4381 tgattaaccg caaaattgag cggttcaacg ttggcagaga taaaccgatt tcactttata
     4441 aggagtaata aaaatggcag gaattaacaa agtgattatt gtcggtcgcc taggtaacga
     4501 cccggaacta cgaacaatgc caaatggcga ttcagtcgcc aaaatcagtg tggcaacttc
     4561 gaccgaatgg acggataaag tatcaggcga taaaaaacaa gccacagaat ggcatagcat
     4621 tatcgccttt cggaatttag ctgacattat tggcaaatac ctgaaaaaag gctcacaagt
     4681 ctatgttgag ggcaaactac gcactcgcaa atggcaagcc caagacggca cagaccgctg
     4741 gacgaccgaa atcatcgccg atcagctaca aatgctcggt agctcaaaca gcggaaacag
     4801 caataataat tgggcaaccg aaccggcagg aaacccaccg ccaaccaatc catataacca
     4861 cgtaatgacg gattcagaat cacgagattt tgatgatgat attccgttct aataatttta
     4921 ttcaaaccct aattaggaga accaaaaatg gcaaaaacaa acgtacctga atttttagat
     4981 gaattagatt gcggtatttt caaagacaag ctcgcaacag ctctttcaga tgtggcgtta
     5041 ggtgtactaa cccacgataa aaagggtaag gtaacggttg aatttagctt agacaaaatg
     5101 gatagcgaca gcccatctgt tcaaattcag cacaaattga gctatgtcaa accgaccaag
     5161 cgtggtaaat cagcagaaga ggatacaacc gccacgccaa tgtatgttca taaaggtggg
     5221 gcattatctg ctacacctga aaaaacagaa acggcagtaa aagaaagccc tagcggttta
     5281 aaggctctat ctaaagctgc tgcataatca attattttca atccaagcca cgttaatgcg
     5341 tggctttatt tttttactca aacaaaaagg aacttaaaat ggacaaaacg acacttcaac
     5401 aaatttcggc tcttgctgtt gctgcaacca aagaacttga aactgatttt ggtgcagtaa
     5461 tgctaccaga aggcattaaa ttacaaaatt tagagccttt ccaagctcac cgtaaccaat
     5521 tccgtgcagc gttttcaacg caacgttttg gtagcctgat tgaatatgct accgccaatg
     5581 ctcaagaaaa tgctcaatgc tttatcgacc aagagaaaat gagtgcggaa attgtatttg
     5641 atatgggcaa ccgagaacaa gcaggtcacg ctaaacatcg tgctaatttg gtgatgaaga
     5701 aaaccgcagc atacaaagct ctatgcgaaa ttaatggctc acgtcgctca cagcgtgatt
     5761 tttctgattt cctcgaagat tggggcatct acttgaacgc ttaccaccac gaagatgaaa
     5821 tcagtattaa aaatgccgtc caagcggtgc gtaaaatgac gattgaccac gcccgaaatg
     5881 aagaacatga actcagcgat ttcgcagcca aaaaatcagc gatggaatcg gtagaagcta
     5941 aatctacact gcaactgcca actcaccttg tatttacttg caatccatac aatggcttag
     6001 acactcgatc atttactctg cgtgtacagg tgctaaccgg tagcggtgaa cctgtactaa
     6061 ctgcccgctt agttcatgct gagcaaattg aagagtctat tgcgactgag tttgcggaaa
     6121 agctagccga tgcacttagc gaaacatcaa tcaaggtcaa tatcggtacg attgcgatct
     6181 agcttgtaag ttagcttata agtttacttg taagttttta atgaaaattg accgcttgtt
     6241 gaatacaagc ggttatttct cgaggaaaat tcacgatgaa aacaacccaa catattttag
     6301 atgaacgtga acaacaacac ggcaactaca acagtttcgc caagatttat ggtggtttac
     6361 gcaaagtaag cgacccgcac gcagagaaac ttacttggcg gcagcaaatc tcggttgaaa
     6421 tgatactgtt taaactggct cgtattttga ataacggctc aaaccaccaa gatagctggc
     6481 aagatattgc cggatatgcc ctattaggcg gcgatattta tactccgcaa tcatcagaca
     6541 ataccaatac caaaggctta ccaaaaccat taacagatag catttatccc gaatcacatc
     6601 ttgataaaaa tgcagtatgg cggctggatt tggagtttga aacgaaagag caagcggtgg
     6661 cggtattgga agccgtaaca ggcaagaaat atagtgaaaa tataacttag ggggaattta
     6721 tgagcaaaga aaaatatttt tcagttgatg tatcaaatga tatacatatc ctaaatctgc
     6781 acgaaacctt agagcaagca aaacaaagct gtttaaatgg tgctgctgaa gcctatgagt
     6841 ttgccagtga aatggatgat tgcgaaaact atgaagccaa tgatttacca tatgcagttt
     6901 atggcgttgt tttaggtaga gcaaaatcag atattcgccc gcttacaagt gaagaaaagg
     6961 aatctgggta ctttgaagag gttgacaatt ttattgaaca accagaactt gtagaaacta
     7021 atggctggat ttcggttaaa gacaaattgc cagaaatcca cgaagaagtg ttggtaacaa
     7081 acaactttat tatgatagct aagtttaatg gcgaaagctg gattacatcg ggaggtatct
     7141 taggtattca gcctgtttac gatgttactc attggcaacc acttcctgaa ccaccaaggg
     7201 aacaataaaa tcctgtaagg ttttagatat gtttagaaat gaactccaag taatggacgg
     7261 taaacgttat gtcgtgcttg aatgccaatt tcgccgcgaa tggaaagttg caatagagtc
     7321 tagaggtact gtaacgagtg gcgaagctat cgaaatttgt cagtattgga tcaagtataa
     7381 aggtgtaaaa cctgagcagc ttaaagttgt tgaagtgcca gatattctga aagaataagg
     7441 acaaatgagt taaaaacggc gacattataa gtgcggtaac acttaaaatg ccagctacgc
     7501 aaagcacgct tgcatatagc catacgccgc cacctagcta ggtaggcgga attttatcaa
     7561 aaaatgaagt aaaagtgtat ttatatgcaa tcattaagat taattgagat ccgctgtaag
     7621 tattgccaaa aattattggc aaaagcgaaa gatgtacaac atttagaaat taaatgtgta
     7681 cgttgtaaat caatcaataa atttaactga attgagtgtc tgagcgttaa gaacgcctga
     7741 acgccatagg agaactatgg caaagcaaat ctttaaacaa gccccacttc cgtttgtagg
     7801 tcagaaacga atgtttctag ctcaggtttc tcaaatttta aatgagaata ttcctgatga
     7861 cggccaaggt tggacaatca tagatgtatt tggtggtagt gggttattag ctcatactgt
     7921 caaacatatc aaaccaaaag ctcacatcat ctacaatgat tatgatggat atgcggagcg
     7981 gctaaagcat attcccgata caaataggct acgcaagcaa atttatgaca ttattgggga
     8041 gagtacgcct aaaaacaagc gattagatcc tgataagaaa tctcaagtta tcaatgttat
     8101 tcagtctttt gatgggtata ttgatgtaaa ttgtgtggct tcttggttgc tatttagcgg
     8161 acagcaaatt aatagtttag agaatctatt taacaaaata ttttggaatg gtattcgcca
     8221 aaccgattat ccaagtgcgg aaggctattt agatggaata gaagttactc acgaaagttt
     8281 tcataaacta ttaccacgtt ttcagcataa agataaggta ttgttattac ttgatccgcc
     8341 atatctttgt actcgccaag aaagctataa gcaagccact tattttgatt taatcgattt
     8401 tttaagatta atcaatttaa ctaaagtgcc ttatatcttt tttagttcaa caaaaagtga
     8461 atttgtgagg ttcgtagatt ttatggttag cgagaaaaaa gataactggc aaacttttga
     8521 aaatgcgaaa tggattaaag ttaaagctag tctaaattat caatctacct atgaagataa
     8581 tttggtttat aaattttgac atttaatgaa tgattgacaa ttaaccgctt gttccgcaag
     8641 cggttat
//
LOCUS       DANJWC010000103         8569 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.103, whole genome shotgun sequence.
ACCESSION   DANJWC010000103 DANJWC010000000
VERSION     DANJWC010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8569
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.103"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            91..864
                     /locus_tag="PWX02_001669"
     CDS             91..864
                     /locus_tag="PWX02_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL1729663.1"
                     /translation="MLKKLSIAALALTLSVSAFAKESLLERINNKGTITVGTEGTYAP
                     YTYHDASGKLTGYDVEVTRAVAEKLDVKVEFKETQWDAMLAGLKGERFDLVANQVALT
                     SPERRATFDKSESYSWDGAALLVREDETKIKTPADVKGVKTAQTLSSNYGEIARENGA
                     DIVPIDGMAQGLLLIQQKRADATFNSHLSLLDYLKKNPNSGLKIVWEKEEKVGAGLIA
                     NKGNDEALAKISKAIVELREDGTLKKLGEQFFGKDISVQ"
     gene            947..1663
                     /locus_tag="PWX02_001670"
     CDS             947..1663
                     /locus_tag="PWX02_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL1729664.1"
                     /translation="MLNDLLLSIPFMTQARVDLVLSGFWPMVQAAFLVSIPLAIASFI
                     IGMLIAVAVALVRISPATGFFHRLLLWLVKGYISIIRGTPMLVQICIVFYGLPAIGIF
                     IDPIPAAIIGFSLNIGAYGSETIRAAISSVPKGQWEAGYTIGMTYMQTFRRIIAPQAF
                     RVAVPPLSNTFIGLFKDTSLASVVTVTEMFRVAQQVANMSYDFLPVYIEAGLIYWCFC
                     WVLFLIQARLEVRFNRFVAK"
     gene            1673..2425
                     /locus_tag="PWX02_001671"
     CDS             1673..2425
                     /locus_tag="PWX02_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL1729665.1"
                     /translation="MLKIRNIHKTFGNNAILRGIDLDIQKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEQGTLEFSGNAPLKIDFAVKQSKKEILALRRKSGMVFQNYNLFPHKTAL
                     ENVMEGPVFVQQQAVEIAQKNAKALLEKVGLADKAELYPFQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNTMKELANEGWTMVVVTHEIKFALDVADVVVVMDG
                     GEIVEQGSPQQLFNHPQHERTKRFLNQIRAGK"
     gene            complement(2641..3174)
                     /locus_tag="PWX02_001672"
     CDS             complement(2641..3174)
                     /locus_tag="PWX02_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDL1729666.1"
                     /translation="MEFLAPVISLIVGFLSFVLVLRTWLQFCRVDPRLPISQTLLKIT
                     EPMVSPVGKLIPTVKNICFPALLIAAALVTLQFILFGVDLPRAVLIGLLSVVKTFGQI
                     LFFTTLIRALMSWVTQGNHPLDYTVAQITEPVLGFIRKLLPRTGMLDFSVMVLGFGLL
                     LLNSLFYQIFGGLWAAA"
     gene            complement(3280..3621)
                     /locus_tag="PWX02_001673"
     CDS             complement(3280..3621)
                     /locus_tag="PWX02_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HDL1729667.1"
                     /translation="MAIQRNARQRKKMHLGEYQELGFLIKFTFAEGTGIDQIDETVDR
                     FIDEVIRPNGLALEANGYLNWEGLVCLEKLGKCDESHRQLVKTWLETNGLKEVQVSEL
                     FDIWWDYPAQA"
     gene            complement(3645..4394)
                     /gene="trmB"
                     /locus_tag="PWX02_001674"
     CDS             complement(3645..4394)
                     /gene="trmB"
                     /locus_tag="PWX02_001674"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDL1729668.1"
                     /translation="MSEKQTFADKKRKTVEQAEFTEDGRYLRKVRSFVLRTGRLSDYQ
                     RDMMNNNWANLGLDYQPMPFNFEQIFGNNNPVVLEIGFGMGKSLVEMAEQNPNRNYIG
                     IEVHTPGVGACIAYALEKGVKNLRVICHDATEILRDSIADGTLGGLQLYFPDPWQKAK
                     HHKRRIVQPEFISRVIRKLSPNGFIHFATDWENYAEHMLEVLRQFESELQNTSATNDF
                     IPRPDFRPLTKFEERGHRLGHGVWDLYFTKK"
     gene            complement(4404..5354)
                     /locus_tag="PWX02_001675"
     CDS             complement(4404..5354)
                     /locus_tag="PWX02_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDL1729669.1"
                     /translation="MNSRILATGSYLPTKVRTNADLEKMVDTSDEWIVTRSGIRERRI
                     AEPDETVATMGAKAAQQALEMAGIEANDIDLIVVGTTTNSHAYPSAACQIQGLLQIED
                     AIAFDVAAACTGFVYALTVADQFVRNGQVKKALVIGSDINSRMLDETDRSTVVLFGDG
                     AGAVILEASEEPGIISTHLHASPDLENNLTLRSQKRGEERSGFIEMQGNATFKIAVNQ
                     LSSVVEETLEANNLQKSDLDWLIPHQANLRIITATAKKLEMEMDQVVVTLDKYANTSG
                     ATVPVALDEAVRDGRIQRGQLLLLEAFGGGWTWGSVLVKF"
     gene            complement(5500..6519)
                     /gene="hemB"
                     /locus_tag="PWX02_001676"
     CDS             complement(5500..6519)
                     /gene="hemB"
                     /locus_tag="PWX02_001676"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDL1729670.1"
                     /translation="MQVIQSQYPLCRMRRLRKHDFSRRLVAENQLTANDLIYPVFVIE
                     GENQRVQVPSMPGVERLTIDQLLVEAAELVKYGVPAVALFPVVGDAKKSLMAEEAYNP
                     QGLAQRAVRALKAQFPELGVITDVALDPFTTHGQDGIIDETGYVLNDVTTEVLVKQAL
                     SHAEAGADIVAPSDMMDGRIGKIREALEQNGFINTQIMAYSAKYASNYYGPFRDAVGS
                     AGNLKGGNKYTYQVDPANGNEGLHEVAMDIQEGADMVMVKPGMPYLDMVWRVKENFGV
                     PTFAYQVSGEYAMHMAAIQNGWLKERECIMEGLLCFKRAGADGILTYFAKRVAKWLYE
                     EAQAK"
     gene            complement(6600..7355)
                     /gene="tatC"
                     /locus_tag="PWX02_001677"
     CDS             complement(6600..7355)
                     /gene="tatC"
                     /locus_tag="PWX02_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HDL1729671.1"
                     /translation="MSQVDESQPLITHLIELRNRLLRSFICVLLVFCGLVYWANDIYT
                     LLATPLTDNLPDGATMIATNVATPFFTPIKLTIVVSVFLSVPFILYQIWAFVAPALYK
                     QEKRLVYPLLVSSTLLFYCGVAFAYYIVFPLVFGFLTSTAPEGVTMATDISSYLDFVL
                     TIFMAFGICFEVPVAIILLCWAGVTTPEDLKAKRPYIIVAAFVIGMLLTPPDIFSQTL
                     LAIPMCLLFEVGVLFARFYRPKDDDEPSEQVAQ"
     gene            complement(7342..7986)
                     /gene="tatB"
                     /locus_tag="PWX02_001678"
     CDS             complement(7342..7986)
                     /gene="tatB"
                     /locus_tag="PWX02_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HDL1729672.1"
                     /translation="MFDIGFSELVLIMIVGLVVLGPKRMPIAIRTVMGWVSTIRGLAA
                     NVQNELAQELKLQELKESIKKAEELNLSSLSPELNKTVEDLKMSATQLQDSLNSAKNE
                     LEKTAAESSTIPKLTDEQVAEIQQKIEQESQVLEAQELQKNAQNKPLEVDSLSEQLNV
                     TRTEKPLSADELAEQAEMDESLLTLESYYPPDDDLATPQSSESAQKSEANNVAS"
     gene            complement(7990..8211)
                     /gene="tatA"
                     /locus_tag="PWX02_001679"
     CDS             complement(7990..8211)
                     /gene="tatA"
                     /locus_tag="PWX02_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HDL1729673.1"
                     /translation="MGGISIWQLLIVVAIIVLLFGTKKLRTLGSDLGESVKGFKKAMA
                     DEKKDAEFEKVEAKDAISAEQKAKEKEQA"
     gene            complement(8322..>8569)
                     /gene="ubiB"
                     /locus_tag="PWX02_001680"
                     /gene_synonym="aarF"
                     /gene_synonym="yigQ"
                     /gene_synonym="yigR"
     CDS             complement(8322..>8569)
                     /gene="ubiB"
                     /locus_tag="PWX02_001680"
                     /gene_synonym="aarF"
                     /gene_synonym="yigQ"
                     /gene_synonym="yigR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249494.1"
                     /note="an Escherichia coli mutant results in accumulation
                     of octaprenylphenol and no ubiquinone; functions in the
                     formation of 2-octaprenyl-6-hydroxy-phenol from
                     2-octaprenylphenol in ubiquinone (coenzyme Q)
                     biosynthesis; similar to eukaryotic proteins that exhibit
                     kinase functions; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HDL1729674.1"
                     /translation="AEFPEALFKALQEQKQINFRLAEINESLKAQNSNANSRFLVLSI
                     SAIVICGAFWKFESLPIWLSVPLLTLFPLVLILSQRK"
BASE COUNT         2451 a         1934 c         1693 g         2491 t
ORIGIN      
        1 tatttcttat tccatttagt tatttgcaag cggtcaattt tccccttaaa tttgcaaaaa
       61 atttatttta ccaattaaaa ggaaatgatt atgttgaaaa aattatcaat tgctgcatta
      121 gctcttaccc tttctgtttc tgcatttgct aaagagagcc tacttgagcg tataaacaac
      181 aaagggacta ttactgtggg tacggaagga acttatgccc cttacaccta tcacgatgcg
      241 agcggtaaat taaccggtta cgatgtagaa gtaacccgtg cggtggctga aaaattagac
      301 gtaaaagtgg aatttaaaga gacacaatgg gacgctatgt tagccggttt aaaaggtgag
      361 cgttttgact tagtggcaaa ccaagtggct ttaaccagcc cggaacgtcg agcgacattt
      421 gataaatccg aatcttacag ctgggacggt gccgccttat tagtgcgtga agatgaaact
      481 aaaatcaaaa cccctgctga tgtaaaaggc gtgaaaactg cccaaacctt gagctcaaac
      541 tatggcgaga ttgcccgtga aaacggtgca gacatcgtgc caattgacgg tatggctcaa
      601 ggcttactgt taattcaaca aaaacgcgcc gatgcaactt tcaatagcca cttgtcgcta
      661 ttagattact tgaaaaagaa cccgaattcc ggcttaaaaa ttgtgtggga aaaagaagaa
      721 aaagtgggtg caggcttaat tgcgaataaa ggcaatgacg aagcattagc gaaaatcagc
      781 aaagcaatcg tagaattacg tgaagacggc acgttaaaaa aattgggcga gcagttcttc
      841 ggtaaagata ttagtgtaca ataatccata ttggattagg cttggggcga attcgcccct
      901 tttattttta aatctccctc aaaacgggag attttaggtt tttcagatgt taaacgatct
      961 acttctctca atccccttta tgacccaagc tcgggtcgat ttagtgctaa gcggtttttg
     1021 gccgatggta caagcggcat ttttagtctc tattccgctg gcaatcgcct catttattat
     1081 cggtatgtta attgccgttg cagttgcatt agtgcgaatt agccctgcaa caggcttttt
     1141 tcaccgcttg ctactgtggc tggtaaaagg ttatatctcg attattcgcg gcacgccgat
     1201 gttggttcag atctgtattg tgttttatgg tttgcctgca atcggcattt ttatcgatcc
     1261 gattccggcg gcaattatcg ggttttcact caatatcggg gcttatggct cggaaacgat
     1321 ccgtgctgcg atctcctccg tgccaaaagg acaatgggaa gccggttata ctatcggtat
     1381 gacttatatg caaacttttc gccgtatcat tgccccgcaa gcgtttcgag ttgctgttcc
     1441 gccacttagc aacaccttta tcggtctgtt taaagatacc tctcttgcct ccgttgttac
     1501 cgtaaccgaa atgttccggg ttgctcaaca ggtagcgaat atgtcttatg acttcctacc
     1561 tgtttatatt gaagccggtt tgatttattg gtgcttctgc tgggtgctat ttttaattca
     1621 ggcgagattg gaagtccgct ttaaccgatt tgtggcgaaa taaggaggaa ttgtgcttaa
     1681 aattcgcaat attcataaaa cctttggcaa caacgctatt ctacgcggca ttgatttaga
     1741 tattcaaaaa gggcaagtgg tagtaatttt aggcccttcc ggttcaggca aaaccacctt
     1801 tcttcgttgc ttaaatgcac tggaaatgcc cgagcaaggc acattggaat ttagtggtaa
     1861 tgctccgctg aaaattgatt ttgccgtaaa acagagcaaa aaagagattt tagcgctacg
     1921 ccgcaaatcc ggtatggtgt tccaaaacta caacctgttc ccgcataaaa ccgcattgga
     1981 aaatgtgatg gaaggaccgg tatttgtgca acaacaagcg gtcgaaattg ctcaaaaaaa
     2041 tgcaaaagcc ctgttagaaa aagtcggact ggcggataaa gcagaactct acccgtttca
     2101 actgtcaggc gggcaacagc aacgggttgg cattgcacga gcattagcaa ttcagccgga
     2161 gctgatgtta tttgatgaac caacttcggc acttgatcct gagttggtgc aagacgtgct
     2221 aaatacgatg aaagaactgg caaatgaagg ctggacaatg gtcgtggtta ctcacgaaat
     2281 caaatttgcc cttgatgttg ccgatgtagt ggtggtaatg gacggcggtg aaatcgttga
     2341 acaaggctcg ccacagcaac tgtttaacca cccacaacac gaacgaacta aacgtttttt
     2401 aaatcaaatc agagcgggta aataagaaac gatagcgcta gcgtccacgc tagtgctatc
     2461 taataaatgg tacaagcggt taattatgta taaattgtta tactgttttg gcacgagcgt
     2521 ggacgctcgc gccatcgtac agtaaaatca gctaacaagg ttccccataa aggataaata
     2581 agacaaggct ccttaatctt ttagattaag gagcttattt ttagatttta gaaatccgat
     2641 ttaagccgca gcccatagtc caccaaaaat ttggtagaac aagctattta gtagcaataa
     2701 gccgaaacct agcaccatta ccgagaaatc taacattccc gttcttggca ggagcttgcg
     2761 gataaaacct aacaccggtt cggtaatttg tgctaccgtg tagtctaatg ggtggttgcc
     2821 ttgagtgacc cagctcatta atgcacgaat caaggtggtg aaaaatagaa tttgtccgaa
     2881 ggttttgacc acacttaata agccgattaa tactgctctg ggtaaatcta cgccaaacag
     2941 aataaactgt aacgttacca aagcggcggc aattaatagt gccggaaagc aaatattttt
     3001 aacggttgga atcagcttgc caaccggtga aaccatcggt tcggtaattt taagtaaggt
     3061 ttgtgaaatc ggtaaacgag gatcaacccg acaaaattgc agccaagttc ttagcaccag
     3121 cacaaaactt aaaaagccaa caataagcga aataacagga gctaaaaatt ccatctcttt
     3181 ttctctttct atacaaaaca agcggtcgtt ttttagaaaa aatttgcaaa ttttcagtaa
     3241 aatctgaccg cttgtatctt attaaattaa ctaggcaaat taagcttgtg ctgggtagtc
     3301 ccaccaaata tcaaataatt cgctcacttg aacctctttt aagccgttag tttctaacca
     3361 agttttcact aattggcggt gagattcatc acatttgcct aatttttcta agcagactaa
     3421 accttcccag tttaagtagc cgttcgcttc aagggctaaa ccgtttggtc gaatcacttc
     3481 atcgataaaa cgatctacgg tttcatcaat ttggtcaatg cctgtgcctt cagcaaaggt
     3541 gaatttaatt aagaaaccta attcttggta ttcacctaag tgcatttttt tacgttggcg
     3601 tgcgttacgt tgaatagcca ttttttctct cctctatgaa taaatcattt ctttgtaaaa
     3661 tataaatccc acaccccgtg tcctaaacga tgccctcgct cttcaaattt ggttagcggg
     3721 cggaaatccg gacgtggaat aaaatcatta gttgctgatg tattttgcaa ctcgctctca
     3781 aattgacgta atacttcaag catatgttct gcgtaatttt cccagtcggt ggcaaaatga
     3841 ataaagccgt tagggcttaa ttttctgatc acacggctga taaattcagg ctgcacaata
     3901 cggcgtttgt ggtgttttgc tttttgccaa gggtctggga agtaaagctg taagccacct
     3961 aatgtaccgt cagcaatgct gtctcgcaaa atttcggtgg catcgtggca aattactctt
     4021 aaattcttca cgcctttttc taacgcataa gcgatacaag cccccacccc gggcgtgtgc
     4081 acctcaatgc cgatatagtt gcgattcggg ttttgctctg ccatttccac cagcgatttt
     4141 cccattccga agccgatttc taacactacc gggttgttat tgccgaaaat ctgctcaaaa
     4201 ttaaagggca ttggctggta atctaagcct aaatttgccc agttgttgtt catcatatca
     4261 cgttgataat cgctcaaacg accggtacgt aatacaaaac tccgcacttt tcgtaaataa
     4321 cgcccatctt cggtaaactc ggcttgctca acggttttac gttttttatc tgcaaaagtc
     4381 tgtttttctg acattttctt gccttaaaac ttcactaata ccgaacccca agtccagcca
     4441 ccgccgaatg cttcaaggag tagtaattgc ccacgttgaa ttcgcccgtc acgcaccgct
     4501 tcatctaatg ccaccggcac tgttgctcca cttgtattgg catatttatc aagggtaacc
     4561 accacttgat ccatttccat ctctaatttt ttcgccgttg cggtaataat gcgtaaattc
     4621 gcttggtgcg gaatcagcca atctaaatcg gatttttgta gattattcgc ttctaaggtt
     4681 tcttctacca cgcttgaaag ttggtttacc gcaattttaa aagtagcatt gccttgcatt
     4741 tcaataaagc cggaacgctc ttcgccacgt ttttggcttc ttaaagttaa gttattctct
     4801 aaatccggcg aagcgtgtaa atgggtcgaa ataatacctg gctcttcact cgcttctaaa
     4861 atcaccgccc ctgctccgtc accaaataac acaaccgtgc tgcggtcggt ttcgtccagc
     4921 attctggagt tgatgtctga accaatcacc aatgcctttt tcacttgccc gttacgcacg
     4981 aattgatcag cgacagttaa agcatacaca aagccggtac aagccgccgc cacatcaaaa
     5041 gcaatcgcat cttcaatctg aagcaaacct tgaatttggc aagctgcact tgggtaagcg
     5101 tgggagttgg tagttgttcc caccacaatt aaatcaatgt catttgcctc aattcctgcc
     5161 atttccagtg cttgttgagc ggcttttgcc cccattgttg ccactgtttc gtcaggctcg
     5221 gcaatacggc gttcacgaat gccggaacgg gttacgatcc attcatcaga ggtatcgacc
     5281 attttttcta aatcagcatt ggtacgcacc tttgtcggta aatagcttcc cgttgctaaa
     5341 attctactat tcatattttt attattctca atcaattaaa tgataatccg ttattatagc
     5401 ttgaaaacca acaaatatat aggctttaca agcggtcatt tttagcaaaa aagttgcatt
     5461 cacgaacgat tttgcactag aaccacctct tgctagtatt tatttcgctt gagcctcttc
     5521 gtatagccac ttcgctactc gcttggcgaa ataagttaga ataccgtctg ctcctgcccg
     5581 tttaaagcag agtaatccct ccataatgca ctcacgctct ttcagccagc cgttttggat
     5641 tgccgccata tgcatcgcgt attcgcccga aacttggtag gcgaaggttg gcacgccaaa
     5701 attctctttc acacgccaca ccatatccaa ataaggcata cccggtttca ccatcaccat
     5761 atctgcccct tcttgaatat ccatcgccac ttcgtgcaag ccttcgttgc cgttagccgg
     5821 atcgacttgg taagtgtatt tattaccgcc ttttaagttg ccggcagaac ctactgcatc
     5881 acggaacggt ccgtaataat tagacgcata tttagccgaa tacgccataa tttgcgtatt
     5941 gataaaaccg ttttgctcaa gggcttcacg aattttgccg attcgaccgt ccatcatatc
     6001 gctcggggca acaatatccg cccccgcttc tgcgtgcgat aacgcctgtt ttaccaatac
     6061 ttcagtagtc acatcattca gtacatagcc tgtttcatca ataatgccgt cttgtccgtg
     6121 cgtggtaaac gggtctaacg ccacatcagt aatcacgcct aattccggaa attgagcttt
     6181 taaagctcgc acggcacgct gtgccaaccc ttgcgggtta taggcctctt ctgccatcag
     6241 tgattttttc gcatcaccca ccacagggaa aagagccacc gcaggcacgc catatttaac
     6301 cagttctgcg gcttcaacta aaagctggtc gatagttaaa cgctctacac ccggcataga
     6361 aggcacttgt actcgctgat tttccccttc gatgacaaaa acagggtaaa tcaaatcatt
     6421 agcggtaagt tgattttctg ccactaagcg gcggctgaaa tcgtgcttgc gtaaacgacg
     6481 catacggcat aaaggatatt gggattgaat tacttgcatt tttaatttcc taattcttaa
     6541 aatacctatt cttgaaataa gaaaggcata gcaaacaggt gctatgcctt ttcaaaatat
     6601 tactgtgcaa cctgctctga tggttcatca tcatctttgg ggcgataaaa ccgagcaaat
     6661 agcacaccaa cttcaaacaa taaacacatt ggaattgcca ataatgtttg cgagaaaata
     6721 tctggtgggg tgagcaacat accaattaca aatgctgcca caataatata agggcgtttt
     6781 gcctttaaat cttcgggtgt ggttacgcct gcccaacata acaagataat cgcaaccggc
     6841 acttcaaagc agatcccgaa tgccataaaa atggttaaaa cgaaatcaag atagctacta
     6901 atatccgttg ccattgttac cccttccgga gcggtagagg ttaaaaagcc aaatacaagc
     6961 ggaaacacaa tataataggc aaaagcgaca ccacaataaa acagcaatgt gctagatacc
     7021 aataatgggt aaaccaaacg cttttcctgc ttgtataaag caggggcgac aaacgcccaa
     7081 atttggtaca aaataaaagg aactgataaa aacaccgaaa ccacaatggt taatttaatt
     7141 ggggtaaaaa acggagtggc tacattggtt gcaatcattg ttgcaccgtc tggtaaattg
     7201 tccgttaaag gtgttgccaa taaggtgtaa atatcattgg cccagtaaac caatccgcaa
     7261 aacaccaaga gcacacagat aaaactacgc agtaatcggt ttctcagctc aattaaatgt
     7321 gtaatcagag gttgagactc atcaacttgc gacattattt gcctccgatt tctgcgctga
     7381 ttctgatgat tgaggcgtag ctaaatcatc atctggcgga tagtaagatt ccaacgttaa
     7441 aagcgattcg tccatttctg cttgttcagc cagctcatca gccgaaagtg gcttttctgt
     7501 ccgcgtaaca ttaagttgct cagataaact atccacttca agcggtttat tttgcgcatt
     7561 tttttgcaat tcttgcgcct ctaacacctg gctttcttgc tcgatttttt gctgaatttc
     7621 tgcgacttgc tcatcggtta atttaggaat tgtgctgctt tccgcagccg ttttttccaa
     7681 ctcatttttt gcagaattaa gcgaatcttg caactgggtg gctgacattt tcagatcttc
     7741 caccgtttta tttaattctg gcgaaaggga gcttaaattc aactcttctg cctttttaat
     7801 gctctctttt aattcttgca gtttcagctc ttgagccagt tcattttgta cgtttgcggc
     7861 taaacctcga attgtactca cccagcccat tacggtacga atcgcaatcg gcatacgttt
     7921 cgggcctagc accaccaagc ccacaatcat aatcagtact aattcagaaa aacctatatc
     7981 aaacacggat taagcctgct ctttctcttt tgctttctgc tctgctgaaa ttgcatcttt
     8041 tgcttccact ttttcaaatt cagcatcttt tttctcatct gccattgctt ttttgaagcc
     8101 ttttacagat tcgcccaaat ccgaacctaa agtacgtaat ttcttcgtac cgaaaagtaa
     8161 tacgatgatt gcaaccacaa tcagaagctg ccaaatacta ataccgccca taaaaactcc
     8221 tttcatttaa tagggaatag ataaaataaa acgctgttta ttatatgtta ctttgacata
     8281 taaaaaagta attttgcaaa aaatttgtca aaaacaaccg cttatttgcg ttgactcaaa
     8341 atcaatacca gtggaaataa agttaataac ggcacactta gccatatcgg caaagattca
     8401 aatttccaaa atgcgccaca gatcacaatc gctgaaattg acagcacaag aaaacggcta
     8461 tttgcgtttg aattctgtgc ttttaaactt tcgttaattt ccgctaaacg gaaattaatt
     8521 tgcttttgct cttgtaaggc tttaaacaat gcctccggaa actccgcaa
//
LOCUS       DANJWC010000104         8557 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.104, whole genome shotgun sequence.
ACCESSION   DANJWC010000104 DANJWC010000000
VERSION     DANJWC010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8557
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.104"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(174..563)
                     /locus_tag="PWX02_001681"
     CDS             complement(174..563)
                     /locus_tag="PWX02_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HDL1729675.1"
                     /translation="MNISQAAEKTGLSTKQIRDYEKAGLLPVPARTQSGYRFYNEADL
                     DRLFFIGNARKVGFSLVQIAELLKLNDNPNRTSREVKQLTEQHIAELEQKINDLNEML
                     KLLRSWSRTCCGNNSPECSILNGLRDH"
     gene            637..837
                     /locus_tag="PWX02_001682"
     CDS             637..837
                     /locus_tag="PWX02_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL1729676.1"
                     /translation="MAIKLQLDGLHCGNCVKSVEKALNDVSGVTQAIVTLENQTAVVE
                     GEVLAEDLIATVEDIGFGAKLA"
     gene            837..2981
                     /gene="cadA"
                     /locus_tag="PWX02_001683"
     CDS             837..2981
                     /gene="cadA"
                     /locus_tag="PWX02_001683"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDL1729677.1"
                     /translation="MTEQQLLIDGMHCAACVRRVEKALLKVENVSFASVNLADQTAFV
                     QGNANSEALIAAVTKIGFGAEILESEEERRAKLQAQTKRVLSHKRTQFIVALVAGFAL
                     MVFGFYKGMQITENNRSLWLVWSLITLATMYFSGREFFSGAYNALKNKSANMDVLVAI
                     STGTAWLYSFWLTLFPQPNAHVYFEASVMIIGFINLGKYLELKAKQRSSLALEKLLDL
                     APKQAVIFEENIAKTIPAKGIKPEMRVQALTGDRLAVDGILESGSIWVDESMLTGEAL
                     PIEKKVGDKVRAGTLVQDGSGVYIAEQVGSQTALARVIHAVRHAQSSKPPLAQFVDKV
                     AAVFVPVVVSIALFAALIWLLVGKDFAFALSIFTTVLIIACPCALGLAIPLSTIAGVA
                     RAAEFGVLVRNIQALQAGSEIDTLVFDKTGTLTTGQMEVTEVITFNNFDRNDLITLTK
                     SLEQHASHPIAKAIVKFAENYPSLDVNEIQVEKGLGIRGKVGEDRVKIGNASFVNFSE
                     KTTACTATLIYIAVNDEIIGRISVQDQLRPEAKETIQRFQAEGYQCLMLTGDRQETAD
                     YFAKELGLNGVIAEVLPEQKAEKIRALQAEGKKVAMIGDGINDAPALAQANVGIAMHN
                     GSDIAVETADLSLMKSGLALVSQILPFSKRVLKNMKQSLLGAFIYNVVAIPVAAGMLY
                     PFTGWLLNPMIAAIAMTLSSITVVLNSQRLLK"
     gene            complement(3034..3819)
                     /gene="truA"
                     /locus_tag="PWX02_001684"
     CDS             complement(3034..3819)
                     /gene="truA"
                     /locus_tag="PWX02_001684"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL1729678.1"
                     /translation="MKIALGIEYDGSRYFGWQRQEAVESVQQQLEQALSVVANSPVEV
                     FCAGRTDAGVHGTGQVVHFETQANRPLQSWCFGTNAHLPDDIAVKWAVAVNEDFHARF
                     SATARRYRYIIFNQKLRSAILPKGVTHFHFPLDERKMHQAGQYLLGENDFSSFRAAKC
                     QSNTPWRNIHHLNVSRQGNYVIVDIQANAFVHHMVRNIVGTLLEIGQGKQPVEWAKWV
                     LEQRDREKAAPTAKAEGLYLVEVHYPEHFGIPQTPLGPLFLAD"
     gene            complement(3892..4581)
                     /gene="folE"
                     /locus_tag="PWX02_001685"
     CDS             complement(3892..4581)
                     /gene="folE"
                     /locus_tag="PWX02_001685"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDL1729679.1"
                     /translation="MTTISPELTITENTLSPEAQKVRQALLAKGIETPTVAHTKDKAS
                     RRAEIQQHMQAVLELLGLDLNDDSLEETPHRLAKMYVDEIFSGLDYATFPKITNIENR
                     MKVSEMVLVDDITLTSTCEHHFVTIDGKVAVAYYPKKWVIGLSKINRVVQFFAQRPQV
                     QERFTEQILTAFQTILETDDVAVYVKATHFCVKCRGVKDTNSYTVTSAFGGVFLEDRE
                     TRKEFLGLLNK"
     gene            4682..5389
                     /locus_tag="PWX02_001686"
     CDS             4682..5389
                     /locus_tag="PWX02_001686"
                     /EC_number="2.7.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid kinase"
                     /protein_id="HDL1729680.1"
                     /translation="MQNIQYLFNADLIPEHQAEFVQSLLTCKNFDNSDRLLGFSKGRG
                     ATWFLDTSAELGINVVLRHYYRGGLFGKIIKDSYFFTRFEATRAYKEFFLLQQMLAWG
                     LPVPRPIAIKVERNFCCYRADIMLEKLENTQDLSKILQSHSLSNEQYEQIGKLIRSLH
                     NHQIHHSDLNIHNILLGNDGKFWLIDFDKCGIEPGENWKQNNLDRLLRSFKKEQGRLN
                     IFFSEENWQAVLRGYTL"
     gene            complement(5444..6541)
                     /gene="apbC"
                     /locus_tag="PWX02_001687"
     CDS             complement(5444..6541)
                     /gene="apbC"
                     /locus_tag="PWX02_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HDL1729681.1"
                     /translation="MNQVNEQQLVEIKAILQQFSHPTLQKDLIALNAFKKAELGGDIL
                     RIEITMPFAWNSGFETLKAETEAKLKQVTGASGVKWVLNYQIATLKRANKHPAVNGVK
                     NIIAVTSGKGGVGKSTTSVNLALALRAQGARVGILDADIYGPSIPHMLGAKDQRPTSP
                     DNKHITPITAHGLQSNSIGYLMEEDNATIWRGPMASSALSQLLNETWWNELDYLVIDM
                     PPGTGDIQLTLSQQIPVTGAVVVTTPQDIALLDAIKGISMFEKVSVPVLGVIENMSMH
                     ICSNCGHHEAIFGTGGAEKIAQKYGTKVLAQLPLHIRLREDLDSGVPTVVAAPEHEIS
                     QAYLALAEKVASELYWQGSVIPSEIMIREVK"
     gene            complement(6538..8091)
                     /locus_tag="PWX02_001688"
     CDS             complement(6538..8091)
                     /locus_tag="PWX02_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL1729682.1"
                     /translation="MLKSLQAVTFVKKIAKVTACILLITSCDQLICPQSIQSVWVENR
                     QSDTLLKRAIYSAVFQLDPHKIKASADAAPLRDLLVGLMAYNRKGDVVPAIAQNWFTE
                     DEKEWLFILDEKAVWSNGQPVTAQDFVASWRRLIDPQNHSVLSKYLIYMGIENAKAIE
                     EQIKSVEELGVEALNAHTLRIRLNHPNHQLPEMLAHSTLLPTYQGIAPTIDNFVSNAS
                     YRLEKLDAQKMVLKARTQEIPFQTIEYDLITTIQNCSAFDIVENPLLNKKTNIVKFPC
                     LCSYYYEFNFDDPLLAKREIREAIRTMVASANIGQEYGVPSQSVIPHSLVKDREKRWN
                     PVVVEQLLTKAGISPYNPLKLTLLYDENSININIANQVTRALSQSELFNITPKMVDWQ
                     TLNKVRGQKDYQIARAGWCADYPDPIVFLQKFHSMSPDNHSGYKSEIVDQKLNRLQTE
                     TLSAEARESLIQEISEQLYSDVAVLPLFQYHHRVGIVSSLLGIDLNNDSEVIYSKDLK
                     RNLANKDNQ"
     gene            complement(8117..>8557)
                     /locus_tag="PWX02_001689"
     CDS             complement(8117..>8557)
                     /locus_tag="PWX02_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchE family NAAT transporter"
                     /protein_id="HDL1729683.1"
                     /translation="FSISLSSFRVAGGILIVSIAMTMISGKLGEHKQNKEEKNEDITE
                     YENIGVVPLAMPIMAGPGAIGSTIVWGTRYHTWEDYVGFSIAIIVFALICYILFRYSA
                     PLVKKLGKTGSNVVTRIMGLILMALGIEIIVAGITTLFPVLLVK"
BASE COUNT         2449 a         1818 c         1711 g         2579 t
ORIGIN      
        1 ttagtgaaat atgaccgctc gtatagctat ttatagcttc cactctccgc caatttttta
       61 gaaacgtcat cattgagtcc atcttattgt gaagcaaata aggttcgttt ttgattattt
      121 gtcataattt ttacctatag actaaagtca ttctatttta ctgcattttt agcttaatga
      181 tccctcaacc cattcaaaat tgaacactca ggactgttgt tgccacagca agtacggctc
      241 cagcttctga gtaatttaag catttcgttt aagtcattta ttttttgttc cagctctgca
      301 atatgttgct cggtgagctg ttttacctct cggctggtgc ggtttgggtt gtcgtttagt
      361 ttcaataatt cggcaatttg cacaagtgaa aagccgactt ttcttgcatt accaatgaag
      421 aacaatcggt ctaaatccgc ttcattataa aaacgataac ccgattgtgt gcgagctgga
      481 accgggagca gtcctgcttt ctcatagtct cgaatttgtt tggtagatag ccctgttttt
      541 tcggcggctt ggctaatgtt catattccct cttgacttta acctaaggtt aaaccttatt
      601 atatcctcga ataaccactc aacataggag aaaataatgg caatcaaatt acaattagac
      661 ggattgcatt gtggtaactg cgtgaaaagt gtcgaaaagg ctttaaatga cgtttcggga
      721 gtaacgcaag ctatcgtaac cttagaaaat caaacagcgg tagttgaagg cgaagttttg
      781 gctgaagatt taattgcaac agttgaagat attggttttg gagcgaaatt agcataatga
      841 ccgaacaaca gcttttaatt gatggaatgc actgtgctgc ttgtgttcgc cgagttgaga
      901 aagcattgct taaagtagaa aacgtcagtt ttgcctctgt taatcttgct gatcaaaccg
      961 cttttgtgca aggaaatgct aattcagaag cattaattgc cgctgttact aaaattggtt
     1021 ttggggcaga gatattagaa agcgaggaag agcgtagagc caagctgcaa gctcaaacta
     1081 aacgtgtgct aagccataag cgtacacaat ttatcgtggc tcttgttgca ggctttgcgt
     1141 tgatggtgtt tggcttttat aagggtatgc agattaccga aaacaatcgc tcgctttggc
     1201 ttgtttggtc tctcatcacg cttgctacaa tgtatttttc gggcagagaa tttttttcag
     1261 gggcttataa cgcccttaaa aataaatctg ccaatatgga cgtgttagtc gccataagca
     1321 caggcacggc ttggctttat tcattttggc ttacgctgtt tccacagcca aatgctcacg
     1381 tctattttga agcgagtgtg atgattatcg gctttatcaa tttaggcaaa taccttgaac
     1441 ttaaagccaa gcaacgctct tcccttgcat tggaaaaatt gctcgatctc gccccgaaac
     1501 aagcggtcat ttttgaagaa aatattgcaa aaactattcc agccaaaggc attaaacctg
     1561 aaatgcgagt gcaagcctta acgggtgatc gcctagcggt tgatggcatt ttagaaagtg
     1621 gctcaatttg ggttgatgaa tcaatgctga caggcgaagc cttgccgatt gagaaaaaag
     1681 taggcgataa agtccgagct ggcacgctgg tgcaagatgg ttcaggggtt tatattgccg
     1741 agcaagttgg ttctcaaact gctttagctc gtgtgattca tgccgttcgc catgcccaaa
     1801 gtagcaaacc accacttgct caatttgttg ataaagtcgc cgctgtattt gtgcctgtag
     1861 ttgtttctat tgcattattt gctgctttaa tttggcttct tgtcgggaaa gatttcgcct
     1921 ttgcactttc catttttacc accgtgctga ttattgcttg cccttgtgcc ttagggttag
     1981 ccattccgct ttcaaccatt gcaggggtag ctcgtgcggc tgaattcggt gtattagtga
     2041 gaaatattca agccttacaa gcagggagtg aaattgatac tctagttttc gataaaacag
     2101 gcacgctcac cacagggcaa atggaggtta cggaagttat taccttcaat aattttgata
     2161 gaaatgattt gattactctt accaaaagtt tagagcagca cgcctctcac cccattgcga
     2221 aagcgattgt aaaatttgcg gaaaattatc cctctcttga tgtcaatgaa attcaagtgg
     2281 agaaaggatt gggcattcga ggcaaggtgg gagaagatag agttaaaatc ggcaatgcct
     2341 catttgtaaa tttttcggaa aaaacaaccg cttgtactgc cacacttatc tatattgcag
     2401 ttaatgatga aataattggc agaatttcgg tgcaagacca acttcgccct gaagcgaaag
     2461 aaacaattca acggttccaa gctgaaggtt atcaatgcct aatgcttaca ggcgatcgcc
     2521 aagaaaccgc tgattatttt gccaaagagc tgggcttaaa tggcgtgatt gctgaagtct
     2581 tacccgaaca aaaagcggag aaaattcgag cgttgcaagc agaaggaaaa aaagtggcga
     2641 tgattggcga tggcattaat gatgccccag cccttgctca agcgaatgtg ggaattgcaa
     2701 tgcataatgg atcagatatt gcggtggaaa ccgccgatct ttctctgatg aaaagcggat
     2761 tagcacttgt aagccaaatt ttacccttct caaaacgagt gctaaaaaat atgaaacaga
     2821 gcctgctagg tgcattcatc tacaatgtgg ttgcgattcc tgtggcagca ggaatgcttt
     2881 atccgtttac tggttggctg ctcaatccaa tgattgctgc gattgcgatg acattgtcct
     2941 ccattacagt ggtgctgaat agccaaagac tgttgaaata attcaagcgg ttggatttgc
     3001 aaaaaaattt acaaactcaa ccgcttgtct taattaatcc gccaaaaaca gcggcccaag
     3061 aggtgtttgg ggaatgccga aatgttcagg gtaatgcacc tcaaccaaat atagcccctc
     3121 agctttcgct gtaggggcag ctttttcccg atcacgttgt tccagcaccc attttgccca
     3181 ttccacaggc tgcttgccct gcccgatttc gagcaacgtt cctacaatat tgcgtaccat
     3241 atgatgcaca aaggcgttgg cttgaatatc aacaatcaca taattgcctt gccgagacac
     3301 attcaaatga tgaatattcc gccacggcgt attagattga catttagcag cacggaaaga
     3361 ggaaaagtca ttctcgccca gcaaatactg ccctgcttgg tgcattttgc gttcatcaag
     3421 gggaaagtga aagtgggtaa cacctttagg taaaattgcc gaccgcagtt tttggttaaa
     3481 aataatatag cggtaacgac gggcggtggc actaaaacga gcgtgaaaat cctcattcac
     3541 cgctaccgcc catttcaccg caatatcatc gggtaaatga gcgttagtgc cgaaacacca
     3601 gctttgtaac gggcggtttg cttgcgtttc aaaatgcacg acctgcccag tgccgtgcac
     3661 accggcatcg gttctgccgg cacaaaacac ctccaccggc gaattggcaa caaccgacaa
     3721 cgcctgctct agctgttgtt gcacgctctc taccgcttct tgtcgttgcc agccaaaata
     3781 acggctgccg tcatattcaa tacctaatgc tattttcatt tcaacctcaa gcacctgatg
     3841 caaaatataa gggcgaaaaa ttttcgcccc cacagtaatt gatatttgca attatttatt
     3901 caataaacct aaaaattctt tacgggtttc tcgatcttct aagaaaacac cgccaaatgc
     3961 agaagttacc gtatagctat tggtgtcttt tacaccacgg catttcacac aaaaatgggt
     4021 cgctttcaca taaactgcga catcgtccgt ttctaaaatg gtctgaaatg cagttaaaat
     4081 ctgttcggta aaacgctctt gcacctgcgg acgttgggca aaaaactgca cgacacggtt
     4141 gattttcgag agtccaatca cccatttttt cgggtaatag gctaccgcca ctttaccgtc
     4201 aatagtgaca aaatgatgct cacaggtaga agttaaagta atatcgtcca ccagcaccat
     4261 ttcactgact ttcattcggt tttcaatatt ggtaattttc gggaaggtag cataatcaag
     4321 cccgctgaaa atttcatcga catacatttt cgccaagcga tgaggcgttt cttccaaact
     4381 gtcatcgttc aaatctaaac cgagcaattc taaaactgcc tgcatatgct gctggatttc
     4441 tgcacggcgg ctggctttat ctttagtatg agcaacagtc ggagtttcaa tgccttttgc
     4501 caacaaggct tggcgaactt tttgagcctc cggcgaaagc gtgttttctg tgatagttaa
     4561 ttcgggcgag atcgttgtca ttggttttcc tctcaaatgt aagcggaata ttctaacaaa
     4621 ttgacatcaa aaaaaatagc tctaaaatga gctatttcag ccataaacac cctctttatt
     4681 tatgcaaaat atacagtatc tctttaatgc cgacctaatt cccgaacatc aagctgaatt
     4741 tgtgcagtct ctcttaactt gcaaaaattt tgacaattcc gaccgcttgc taggtttctc
     4801 aaaagggcga ggtgcgactt ggtttttaga tacctctgcc gaactaggta tcaatgtagt
     4861 gcttcgccat tattatcgtg gcggcttgtt cggtaaaatc atcaaagaca gttacttttt
     4921 tacccgtttt gaagccaccc gagcttataa agaatttttc ttactgcaac aaatgttagc
     4981 ttggggctta cctgtgcctc gcccaatagc gatcaaagta gaaagaaatt tttgttgcta
     5041 tcgtgccgac attatgctcg aaaagctaga aaacacacaa gatttaagca aaatcttgca
     5101 atcccattct ttatcgaacg aacaatatga gcagataggg aaactgattc gcagtctgca
     5161 taaccatcaa attcaccatt ccgacttaaa tatccataat attttgcttg gtaatgatgg
     5221 caaattttgg ctgattgatt ttgataaatg cggcattgag ccgggcgaaa attggaagca
     5281 aaataaccta gaccgcctgt tacgctcgtt taagaaagaa caagggcgat taaatatttt
     5341 cttcagcgaa gaaaattggc aggctgtttt gagaggatat actctctaag aaacaataat
     5401 ggcacaagct tagaaacttg tgccatcaaa tcatcaacat taactacttc acttcacgaa
     5461 tcataatttc agacggaatc acactgcctt gccagtaaag ttcagaagct actttttccg
     5521 ctaatgcgag gtaggcttgg ctgatttcgt gttccggagc tgccaccacc gttggcacac
     5581 cgctatctaa atcttcacgc agacgaatat gtagtggtaa ttgtgctaac actttagtgc
     5641 catatttttg ggcgattttt tccgctccgc cggtgccgaa aattgcctca tggtgtccac
     5701 agtttgagca gatatgcatt gacatatttt caatcacccc aagcactggt actgagactt
     5761 tctcaaacat tgagatccct ttaatcgcat ctaataaggc aatatcttgt ggggttgtta
     5821 ctaccaccgc accggtcacc ggaatttgct gtgaaagcgt aagctgaata tcacccgtac
     5881 ccggtggcat atcaatgacc aagtaatcca attcattcca ccaagtttca tttaaaagct
     5941 gacttaacgc actgcttgcc atcgggccac gccaaatggt ggcgttatct tcttccatta
     6001 aatagccgat agaattagat tgcaagccgt gagcggtaat cggcgtgatg tgcttgttat
     6061 caggagaagt tggacgttgg tcttttgctc ccaacatatg cggaatagat gggccgtaaa
     6121 tatccgcatc taaaatcccc actcttgccc cttgtgctcg taaggctaaa gcaagattaa
     6181 ctgaggtcgt tgatttccct accccacctt ttcctgaagt gaccgcaatg atatttttta
     6241 ccccattgac tgccgggtgt ttattcgccc gttttaaggt cgcaatttgg taatttaata
     6301 cccattttac accgcttgca cctgttactt gctttaattt agcctctgtt tcggctttta
     6361 aggtttcaaa accactgttc cacgcaaatg gcatcgtgat ttcaattcta agaatatcgc
     6421 cccctaattc tgctttttta aacgcattaa gggcaattaa atctttctgt aaagtcgggt
     6481 gggaaaattg ttgcaagatt gctttaattt caacaagttg ctgttcattt acttggttca
     6541 ttggttatcc ttgtttgcta aattgcgttt taaatctttg ctataaatta cttcgctatc
     6601 gttattcaaa tcaataccca aaagggagga aacaatccca acccgatgat ggtattgaaa
     6661 taatggcaat acggcaacat cgctataaag ctgctcgcta atttcttgaa taagtgactc
     6721 tctcgcttct gcactaagcg tttctgtttg taaacgattt aatttttgat cgactatctc
     6781 acttttatag ccggaatgat tatccgggct cattgaatga aacttctgca aaaatacaat
     6841 cggatcagga taatctgcac accagcctgc tcgtgctatc tgataatctt tttgtcccct
     6901 tactttattt aaagtctgcc aatcaaccat tttaggtgta atattaaata attctgattg
     6961 agaaagggct cgagtaactt ggttagcaat attaatatta atgctattct catcatataa
     7021 caaagtcagc tttaaaggat tataagggct aattcctgct tttgttaata gctgctcaac
     7081 cacaacagga ttccaacgct tttctctatc ttttactaag ctatgtggaa ttacagactg
     7141 actcggaacc ccatattctt gcccaatatt tgctgaagcc accattgtcc ttattgcttc
     7201 acgaatctct cgtttagcta ataatggatc atcaaaattg aactcatagt aataagagca
     7261 caaacacgga aatttcacga tattcgtttt tttatttaat aaaggattct cgacaatatc
     7321 aaaagcagag cagttttgga tagttgtaat taaatcatac tctatagttt gaaagggaat
     7381 ttcttgggtt cgagccttta acaccatttt ttgagcatct agtttctcga gacggtaaga
     7441 cgcattgctc acgaagttat ctatggttgg agcaatccct tgataagtag gtaataatgt
     7501 ggaatgtgcc agcatttcag gcaattggtg atttgggtga tttagccgaa ttctcaatgt
     7561 atgcgcattt aatgcctcta caccgagttc ttcaactgac tttatttgct cttcaatcgc
     7621 ttttgcattt tctataccca tatagatgag atatttagac aatacagaat gattttgagg
     7681 atctattaaa cgtcgccagc ttgctacaaa atcttgtgca gtcacaggtt ggccattaga
     7741 ccacacagct ttctcatcta aaataaacaa ccactctttt tcatcttcag taaaccaatt
     7801 ctgagcaatg gccggaacga catcaccttt tctgttgtag gccattaaac cgactaatag
     7861 atctcttaaa ggagcagcat ctgcagaagc tttaatttta tgtgggtcga gttgaaatac
     7921 agcagaataa attgcacgtt tgagtaaagt atcactctgc cggttttcta cccagacaga
     7981 ttgaatggat tgagggcaaa ttaattgatc acaagatgta ataagcagaa tgcaagcggt
     8041 aacttttgca atttttttta caaatgtaac cgcttgtaag gatttcaaca taaactcccc
     8101 aaaggttaat taagatttat tttacaagaa gcacaggaaa taaagttgta attcctgcca
     8161 caataatttc aattcccagc gccattaaaa tcagccccat gatacgagta acgacattag
     8221 atcctgtttt gcctaatttt ttcaccaaag gggctgaata gcggaataaa atataacaaa
     8281 ttaatgcaaa tacaataatc gcaatgctaa agccaacata gtcttcccaa gtatggtagc
     8341 gggttcccca cacaatggtt gagccaattg cccctggtcc tgccataatc ggcattgcta
     8401 atggcacgac accgatattt tcgtattcag taatatcttc atttttctct tctttatttt
     8461 gtttgtgttc acctaattta ccgctaatca tcgtcatcgc aatgcttaca atcaaaatgc
     8521 caccagctac tctaaaagag ctaagtgaaa tgctaaa
//
LOCUS       DANJWC010000105         8510 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.105, whole genome shotgun sequence.
ACCESSION   DANJWC010000105 DANJWC010000000
VERSION     DANJWC010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8510
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.105"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            90..806
                     /locus_tag="PWX02_001690"
     CDS             90..806
                     /locus_tag="PWX02_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL1729684.1"
                     /translation="MKKTLLTLLFGCVVTAQAQDIKFVMEPSYPPFEMTDEKGEIIGF
                     DVDIANAICKEMNANCTFHSQPFDSLIQSLKQKQFDAAISGMGIAEPRKKQVLFSEPY
                     FPSSAAFIAKKDIDFAKVKTIGVQNGTTYQHYLAKEKKEYNVKSYASYQNAILDVQNG
                     RIDAIFGDVPVLAEMARKHEGLDFVGEKINNPNYFGDGLGIATHLKNQVLVDQFNAAL
                     KTIKENGEYQKIYDKWMGGK"
     gene            complement(866..1744)
                     /locus_tag="PWX02_001691"
     CDS             complement(866..1744)
                     /locus_tag="PWX02_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HDL1729685.1"
                     /translation="MKNLYKWPAAQSLYPNQGKKSYAGKRFRYRLRSWLHHSKIKQFE
                     QFVTQNPQLIPLLNARPNYSYPVAHRFLDKRFSAKQRLQKITDNLLFLPQKLAHLPPL
                     WEQAVNFGEIIADFELWLNINEHQPMEGFWALELRHRPTNQLVYLLTFGKLDEALLIA
                     VIQGPNFEDSKELVKQLTKSCHGLRPAYLMVETMKALTAVLGYQSLLGIPQKYQNKSR
                     WVQSKRYVVDYDAIFAESGGKPGNYWQLPLELESKKIDEIPSKKRSMYRKRYEMLANL
                     KQTMELMLKTYTGLNG"
     gene            complement(1820..3145)
                     /gene="hslU"
                     /locus_tag="PWX02_001692"
     CDS             complement(1820..3145)
                     /gene="hslU"
                     /locus_tag="PWX02_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HDL1729686.1"
                     /translation="MMSMTPREIVSELDAHIIGQKDAKRAVAIALRNRWRRMQLPEDL
                     RQEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LADVSMKLVRQQAVEKNKMRAQDAAEDRILDVLLPPAKDQWGNVQESDNNSTRQTFRK
                     KLREGQLDDKEIEIDVAAQVSVEIMTPPGMEEMTSQLQSLFEGMSPSKTKKRKMKIKD
                     ALKVMLDEEAAKLVNPEELKQQAIEAVEQHGIVFIDEIDKICKKGEHSGGDVSREGVQ
                     RDLLPIIEGSTVNTKHGMVKTDHILFICSGAFQVARPSDLLPELQGRLPIRVELKSLT
                     KEDFERILTEPNASLTLQYRELMKTEGVEIEFTKGGISRIAESAFRVNEKTENIGARR
                     LHTVLERLMDGISFDASERRGEKIIIDENYVSEALNDVVENEDLSRFIL"
     gene            complement(3197..3718)
                     /gene="hslV"
                     /locus_tag="PWX02_001693"
     CDS             complement(3197..3718)
                     /gene="hslV"
                     /locus_tag="PWX02_001693"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDL1729687.1"
                     /translation="MTTIVCVRKDGKVAIGGDGQATLGNCIEKGTVRKVRRLYKDKVV
                     TGFAGSTADAFILRDLFEKKLELHQGHLVKSAVELAKEWRTERALRRLEAMMIVANDS
                     EFLLVSGSGDVIEPEQDVLAIGSGGNYAKAAALALLRTENNLSAKEIVAEALKIAGDI
                     DIYSNHKHVIEEV"
     gene            complement(3779..4492)
                     /gene="sfsA"
                     /locus_tag="PWX02_001694"
     CDS             complement(3779..4492)
                     /gene="sfsA"
                     /locus_tag="PWX02_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDL1729688.1"
                     /translation="MKFPPLSHGILLRRYKRFLADIQLPNGEQITLHCPNTGAMTGCA
                     NPGDTVWFSTSHNPKRKYAHTWELTQTQAGDFICVNTLRANQLVQEALENQWISELAD
                     YQHILPEQKYGSENSRIDFLLKSDLLPDCFVEVKSTTLLTENGVGMFPDAKTERGQKH
                     LRELTKIAEQGLQAVIFFAILHTGIERFEVAKQIDPKYAELFAQAIAQKVQVLSYKMA
                     ISLENGQPIATTLTKKCEF"
     gene            complement(4508..5377)
                     /locus_tag="PWX02_001695"
     CDS             complement(4508..5377)
                     /locus_tag="PWX02_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDL1729689.1"
                     /translation="MLYLLIATLIWASSFIVGKVAISAFDPVQIMQIRLTLAAFIALP
                     FFIPAYKKIQKNQHIKVWLISFLTFPLCVILQFSGLKLTSAASAVALLGLNPLLTVLV
                     SYFCFKKQATRLDFILSLFACLGILIMASGSDDSGSINVLGLLLVTLGSLSFIVGMYL
                     SKEILQSVKVIDLTKITLVQGAITALPITLLFAENWQLPTSLSAWASVAYLGIICTSL
                     AILLWNKGISQSSPILAGILLALEPVFGLLMALVFLSEQLSLVTWVGIIIVICTALLS
                     VLLPMMKRYKRLI"
     gene            complement(5378..5770)
                     /locus_tag="PWX02_001696"
     CDS             complement(5378..5770)
                     /locus_tag="PWX02_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL1729690.1"
                     /translation="MKSPILGFHHIALIVSDYEKSKHFYTRILGAEIIAETYREQRAS
                     YKLDLRFKDGSQIELFSFPETPPRFTSPETCGLRHLAFRVENIEYAIAFLEQHQLPHE
                     GIRIDEITGKRFTFFRDPDDLPLEFYEV"
     gene            5854..6999
                     /gene="mutY"
                     /locus_tag="PWX02_001697"
     CDS             5854..6999
                     /gene="mutY"
                     /locus_tag="PWX02_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDL1729691.1"
                     /translation="MNSNSPFPLAKAKASDPFAKAVLAWFEQYGRKHLPWQQNKTLYQ
                     VWLSEVMLQQTQVATVIPYFARFMERFPTVIDLANASIDEVLHLWTGLGYYARARNLH
                     KAAIQIRDEFGGEFPTRFDDVLALTGVGRSTAGAILSSVLDAPHPILDGNVKRVLSRY
                     FAVEGWAGEKAVENRLWQLSESVTPDTQVADFNQAMMDLGAMICTRTKPKCLLCPLQE
                     NCKTNAMQAWDSFPAKKPKKTLPERQTYFLVLRSGSKVLLQKREAKGIWGGLFAFPQF
                     ESLEALKRSDFMQNLQISQQLTAFRHTFSHFHLDITPVLVETDLKKTSENSPLVVEEK
                     AGNYLSKVSSNADYWYDLHQHNEVGLATPVKRILDELAFSVKNEIKD"
     gene            7003..7272
                     /locus_tag="PWX02_001698"
     CDS             7003..7272
                     /locus_tag="PWX02_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HDL1729692.1"
                     /translation="MARTVFCEYLKQEAEGLDFQLYPGELGKRIFDSISKQAWSEWIK
                     KQTMIVNEKKLSMMNPEHRKLLEQEMVNFLFEGKEIIIEGYKPVE"
     gene            7289..8380
                     /gene="mltC"
                     /locus_tag="PWX02_001699"
     CDS             7289..8380
                     /gene="mltC"
                     /locus_tag="PWX02_001699"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HDL1729693.1"
                     /translation="MKKYAKYLPMLAIIPFLIACGSNKSTKKPKSTRVDYSKDTNGLD
                     ILAGQFSHNIDDIWGSNELLVASKKDYVKYTDKFYTRSHISFEEGVITIETLGNQNHL
                     RNSIIHTLLMGRDPKGIDLFASGDAPISSNPFLNGQVEDQFGRNITNVAIANDFATYL
                     LQNKVQTRRLKNGQTVSFVTIKMVAGHIEVRARQYLPLVRKMAKQYGIEPSLILGIME
                     VESAFNPYAISYANAIGLMQVVPRTAGRDIFARKGMGGQPSREYLYNPSNNIDAGTLY
                     LTILRDEYLDGIEDPVAKRYAMISAYNSGAGAVLRVFDSDRYSAIDRINDLSPDAVYR
                     ILTTAHPSSQARNYLRKVTTAREKYRNIR"
BASE COUNT         2478 a         1721 c         1801 g         2510 t
ORIGIN      
        1 taaaacaaac tttatgaaaa gaagactttt tttaagaaag taactgcgtt aaagtggtaa
       61 tccttataat gaaaacacag ggacttttta tgaaaaaaac acttttgacc cttttattcg
      121 ggtgtgtggt tactgcgcag gcacaagata tcaaatttgt gatggagcca agctatcctc
      181 catttgaaat gaccgacgag aagggagaaa ttatcggttt tgatgtggat attgcaaatg
      241 ctatctgtaa agagatgaat gcaaattgta cattccatag ccaacctttt gatagcttaa
      301 tccaaagcct aaaacaaaaa caatttgatg ctgcaatttc aggtatgggt attgcggaac
      361 cacgtaagaa acaagtatta tttagcgagc cttactttcc aagctctgct gccttcattg
      421 cgaaaaaaga tatagatttt gctaaggtga aaacgattgg tgtgcaaaat gggactactt
      481 accaacacta tttagcaaaa gagaaaaaag agtacaatgt gaaatcttat gcctcttatc
      541 aaaatgctat tttagatgtg caaaatgggc gaattgatgc tatttttggc gatgtgccgg
      601 tattggctga gatggcaaga aaacacgaag ggctagattt tgtaggggag aaaatcaata
      661 atccaaacta ttttggtgat ggtttaggca ttgcaactca cttgaaaaac caagtattag
      721 ttgatcaatt caatgcagca ttaaaaacga ttaaagagaa tggcgaatat caaaaaatct
      781 atgataaatg gatgggcggc aagtaacttg taagcggtca ttttttgcaa agttttgcag
      841 attttgaccg cttgtattct ttgatttacc catttaagcc agtataagtt ttaagcataa
      901 gctccatggt ctgctttaaa ttagctagca tttcataacg cttgcgatac attgagcgtt
      961 ttttgcttgg aatttcatct atttttttac tttccaattc aaggggtaac tgccaataat
     1021 ttcccggctt tccgcccgat tccgcaaaaa tagcatcata atccactaca tagcgcttac
     1081 tttgcaccca gcgggattta ttctggtatt tctgtggaat gcctagtaaa ctctgatagc
     1141 caagaacagc cgttaaagcc ttcatcgttt caaccattaa ataggccggg cgtaagccgt
     1201 ggcaactttt ggttaattgt ttcaccagct cttttgaatc ttcaaaattc ggaccttgaa
     1261 ttacggctat caacaaggct tcatctaact taccaaatgt taataaataa actagctgat
     1321 tagtcggtct gtggcgaagt tctaatgccc aaaaaccttc catcggctga tgttcattga
     1381 tgttaagcca taactcaaaa tctgcaataa tttcaccaaa attgaccgct tgttcccata
     1441 gaggtggtaa atgagccaat ttttgcggta aaaacagcag gttatctgtg attttttgta
     1501 agcgttgttt tgcactgaaa cgcttatcta aaaagcggtg agccacgggg tagctgtaat
     1561 ttggtcgagc atttaagagc ggaatcagtt gtggattttg tgtaacgaac tgctcaaatt
     1621 gcttgatttt ggaatgatgc aaccaagagc gaagacggta acgaaaacgt ttccctgcgt
     1681 aagacttctt tccctgattt ggataaagcg attgtgcagc aggccatttg tatagatttt
     1741 tcatttttac aactaacaat atgaaaagcg gctaattcgc aagatttttc gctaaaataa
     1801 ccgcttgtat aacttaaaac tacaaaataa agcgacttaa atcttcgttt tccaccacgt
     1861 catttaacgc ttcggaaaca tagttttcat caatgatgat tttttcacca cggcgttcgc
     1921 tggcatcgaa cgaaatgccg tccatcaaac gctcaagcac ggtgtgtaaa cgtcttgccc
     1981 cgatgttttc ggttttctcg ttcacacgga atgcactttc tgcaatacgg ctgataccgc
     2041 ctttagtgaa ctcaatctcc acgccttcag ttttcatcaa ctcacgatat tgcaaggtta
     2101 atgaggcatt cggttcggtt aaaatacgct caaaatcttc tttggtaagc gattttaact
     2161 ccacacgaat cggcaagcga ccctgtaatt ctgggagtaa atcagacggg cgagccactt
     2221 ggaatgcccc cgagcagata aacagaatat ggtcggtttt caccataccg tgcttggtat
     2281 ttaccgttga gccttcaatg attggaagta aatcacgctg aacaccttca cgagaaacat
     2341 ctccacccga atgctcgcct tttttacaga ttttgtcgat ctcatcaata aagacaatac
     2401 cgtgctgttc caccgcttca atcgcttgct gtttcagctc ttcaggattt actaatttcg
     2461 ccgcttcttc atcaagcatc acctttaacg catctttgat tttcattttg cgttttttgg
     2521 ttttgcttgg cgacattccc tcaaacagcg attgtaattg cgaggtcatc tcttccattc
     2581 ccggcggggt cataatctca acgctcactt gagctgccac gtcaatttca atctctttat
     2641 cgtctaattg cccttcacgc aattttttgc ggaaagtttg gcgggtagaa ttattgtcgg
     2701 attcctgcac atttccccac tgatctttcg ccggtggcaa taatacgtct aaaatgcgat
     2761 cttccgctgc atcttgtgca cgcattttat ttttttcgac cgcttgttgg cggactaatt
     2821 tcatcgacac atcagctaga tcacggataa tcgaatccac ttctttaccc acatacccca
     2881 cttcagtgaa tttggttgcc tcaactttga taaaaggggc attggcaagc ttcgccaaac
     2941 ggcgggcgat ttcggtttta cccacaccgg tcggaccgat cattaaaata tttttggggg
     3001 tcacttcttg gcggagatct tccggcaact gcattcttct ccagcggtta cgcaaagcaa
     3061 ttgctaccgc acgttttgca tctttttgtc cgataatgtg tgcgtctaat tctgagacga
     3121 tttcacgggg tgtcattgac ataatgttat tccttaaatt aattaccctc tcccacaagg
     3181 ggagagagtt atttaactaa acttcttcaa tcacgtgctt gtggttactg taaatatcaa
     3241 tatcgccggc aatttttaac gcttccgcaa cgatttcttt tgcagataaa ttattttcgg
     3301 tgcgtaataa cgcaagagca gcggctttcg catagttacc gcctgaacca atcgccagta
     3361 catcttgctc cggctcaatc acatcgcccg aacctgaaac caataaaaac tcgctgtcat
     3421 tggcgacaat catcatcgct tctaaacggc gtaaagcacg ctcggtacgc cactcttttg
     3481 ccagttccac tgccgatttc actaaatgcc cttggtgtaa ctccaatttt ttctcgaata
     3541 aatcacgcaa aataaaggca tcagcggtcg agccggcaaa gcctgtcacc actttgtctt
     3601 tatacaaacg gcgaactttc cgcaccgtgc ctttctcgat acaatttcca agcgttgcct
     3661 gaccatcacc accgatagcc acttttccgt ctttacgcac gcatacaatc gttgtcatac
     3721 taaatccttc tttttaaaaa ctttggctta tatggggata tgctgtgtgt tttcaagctt
     3781 aaaactcaca tttctttgtt aaggttgtcg caataggttg cccgttttcc aatgaaattg
     3841 ccattttata ggagagaact tgcacttttt gtgcaatggc ttgagcgaat aattcggcat
     3901 atttggggtc tatctgcttt gccacttcaa accgttcaat acctgtgtgc aaaatagcga
     3961 aaaaaatgac cgcttgtaag ccctgttcag cgattttcgt cagctcccgc aaatgttttt
     4021 gcccccgctc ggttttggca tcggggaaca ttcccacacc gttttcggtt aaaagggtgg
     4081 tagatttcac ttccacaaaa caatccggca gaaggtccga tttgagcaaa aaatcaatcc
     4141 gactgttttc actgccatat ttttgttcag gcagaatgtg ctgataatcc gcaagctcgc
     4201 taatccattg attttccaag gcttcttgta caagctgatt ggctctcaag gtattgacac
     4261 aaataaaatc ccccgcttgg gtttgggtta gttcccaagt gtgagcatat ttacgtttgg
     4321 ggttatgaga ggtggaaaac caaactgtat caccggggtt ggcacagcct gtcatcgcac
     4381 cggtatttgg gcaatggagg gtaatttgtt cgccatttgg caactgaata tcggcaagaa
     4441 aacgtttata acggcgaagt aaaatgccgt gggaaagagg gggaaatttc atttgcaaaa
     4501 aatcctgtta aattaaccgc ttgtatcttt tcatcatcgg tagcaaaacg gagagtaagg
     4561 cggtgcaaat aacaataata atcccaaccc aagtcactaa actgagctgt tctgataaaa
     4621 agaccaatgc cattagcagt ccgaataccg gttcaagtgc gagtagaata cctgcaagaa
     4681 taggcgaaga ttggctgatg cctttgttcc ataacaatat tgccaagctg gtacaaataa
     4741 tgcctaaata tgctacgctc gcccatgctg agagactggt tggcagttgc caattttctg
     4801 caaagagtaa ggtaataggt aaggcggtaa ttgccccttg caccagcgtg atcttggtta
     4861 aatcgatcac ttttactgat tgcagaatct ctttgctgag gtacattccc acaataaaac
     4921 ttaaactgcc taaggttaca agcaataatc ctaatacatt gattgaaccg ctatcatcgc
     4981 tacccgatgc cataattaaa atccccaagc aggcaaataa acttaggata aaatcgagcc
     5041 tggttgcctg ttttttgaaa caaaaatagc tgactaacac agttaaaagc gggtttagcc
     5101 cgagtagtgc cacggcactt gctgcggaag ttaatttcaa gccggaaaat tgtaagatca
     5161 cgcacagagg aaaagtgaga aaactaatca gccagacttt aatgtgctga tttttctgga
     5221 tttttttata cgcaggaata aaaaacggca gagcgataaa cgccgctaac gtcagccgaa
     5281 tttgcataat ttggacaggg tcaaaggctg aaatcgccac tttacctaca atgaatgaac
     5341 tcgcccaaat cagggttgca atcagtaaat ataacattta gacctcgtaa aactctaacg
     5401 gcagatcgtc cggatctcgg aaaaacgtga agcgtttgcc ggtgatctca tcaattcgga
     5461 tcccttcatg cggtaattga tgttgttcta aaaatgcgat ggcatattca atattttcca
     5521 ctcgaaatgc caaatggcga agcccgcagg tttcgggcga ggtgaaacga ggcggcgttt
     5581 caggaaaact gaataattca atttgtgagc catctttaaa gcgtaaatct aatttatagc
     5641 tggcacgttg ctctcggtag gtttctgcga tgatctctgc ccctaaaatg cgggtgtaga
     5701 aatgtttgga tttttcgtaa tcggaaacaa ttaatgcaat gtggtggaaa cctaaaatgg
     5761 gcgatttcat cttttttcct taatgtgtaa aatttttggt aaaactgacc gcttgcagta
     5821 tgataattta tttttcggat ttcaagattt ttaatgaaca gcaattcccc atttccttta
     5881 gcgaaagcaa aagcttctga tccgtttgcc aaagctgttt tagcttggtt tgagcaatat
     5941 gggcggaaac atttaccttg gcagcaaaat aaaacgctgt atcaggtttg gttgtcggag
     6001 gtaatgttgc aacaaactca agtagcgacc gttattcctt attttgcacg ttttatggaa
     6061 cgatttccca ccgtaattga cttagctaat gcctcgattg atgaagtatt gcatttgtgg
     6121 acagggctgg gttattacgc tcgggcaaga aatttgcata aagcggcaat tcaaattcga
     6181 gatgagtttg gcggtgaatt tcctacccga tttgatgatg tgttggcatt aaccggagtg
     6241 ggcagaagta ctgcaggggc gattttgtct tctgtgttag atgccccaca cccgatttta
     6301 gatggcaatg tcaaacgggt gttaagccgc tattttgcag tagaaggctg ggcgggtgaa
     6361 aaagcggtgg aaaatcgcct ttggcaatta agcgaaagtg tgacgcctga tacccaagtg
     6421 gcagacttta accaagcgat gatggattta ggggcgatga tttgcacccg tacaaagcct
     6481 aagtgcttgc tctgcccgct acaagaaaat tgtaaaacga atgcaatgca agcgtgggat
     6541 agcttccctg ccaagaaacc gaaaaaaacc ttgccggagc gtcaaaccta ttttttagtg
     6601 ctgagaagcg gctcgaaagt gctgttacaa aaacgggaag caaaaggcat ttgggggggc
     6661 ttatttgcgt ttccgcaatt tgagagctta gaggctctca agcggtctga ttttatgcaa
     6721 aatttgcaaa tttcacagca attaactgca tttaggcata cttttagcca ttttcatttg
     6781 gatattaccc cggttttagt agaaaccgat ttgaaaaaaa caagtgaaaa ttcaccgctt
     6841 gttgtcgaag aaaaagcagg aaattatctt tctaaggtat cttcaaatgc ggattattgg
     6901 tatgatttgc accaacataa cgaagtgggt ttagcaacgc cggttaaacg tattttagat
     6961 gagctggcat tcagtgtgaa aaatgagatt aaggattaaa ttatggctcg taccgtattt
     7021 tgcgaatatt taaaacaaga ggcagaaggt ttagattttc aactttatcc gggggagctg
     7081 ggtaagcgta tttttgactc aatcagcaag caagcgtggt ctgagtggat taaaaaacag
     7141 acaatgatcg tcaatgagaa aaaactcagt atgatgaacc ccgaacaccg taaactgctc
     7201 gaacaagaga tggtcaattt tttatttgaa ggtaaagaga tcatcatcga aggctataag
     7261 ccggtcgaat aataaaggtt aaatcacaat gaaaaaatat gctaaatatt tgccgatgtt
     7321 ggcaattatt ccgtttctta ttgcttgtgg gagcaataaa tcgactaaga agccaaaatc
     7381 tacccgagta gattattcta aagataccaa tggtttggat attttggcag ggcagttttc
     7441 tcacaatatt gatgatattt ggggaagtaa cgagctgtta gttgccagta agaaagatta
     7501 tgtgaaatat acggataagt tttacacccg tagccatatt tcgtttgaag aaggggtgat
     7561 tacgattgag actttaggga atcagaatca tctacgcaat tcgattattc atactttatt
     7621 aatgggcaga gacccgaaag ggattgattt atttgcctca ggcgatgcac cgattagctc
     7681 taaccctttc ctaaacggac aggttgaaga tcaattcggg cgaaatatta ccaatgttgc
     7741 catcgccaat gattttgcga cttatttgtt gcaaaataag gtgcaaacac gccgtttgaa
     7801 aaatggtcaa actgtgagct ttgtaacgat taaaatggtg gcaggacaca ttgaagtacg
     7861 tgccagacaa tatttaccgc tggtgcgtaa aatggcgaag cagtatggta ttgagccaag
     7921 tttgatttta gggattatgg aagtcgaatc agcattcaac ccttatgcca tcagctatgc
     7981 caatgcgatt ggcttaatgc aggttgtacc tcgtaccgcc gggcgtgata tttttgcccg
     8041 taaaggtatg ggagggcagc caagtcgtga atatctttat aatccatcaa ataacattga
     8101 tgcaggtacg ctttatttga ctattttgcg tgatgaatac ttagatggaa ttgaagatcc
     8161 tgtggctaaa cgctatgcaa tgatttctgc atacaatagc ggagcaggtg cggtattacg
     8221 agtgtttgat tcagaccgat actctgcgat tgatagaatt aacgatcttt cgcctgatgc
     8281 ggtttatcga attttaacca ctgctcatcc atcttctcaa gctagaaatt atttaagaaa
     8341 agtaaccacc gccagagaga aatatcgcaa tattcgttag tgtgtgaatt atgctgtttt
     8401 ttagccgaaa agtataaaaa aagcacattt gagcagcggt ttttttaaat ttgcaaaaaa
     8461 acgtttgacg ttgcaagctg aaatcagcat aatgcaccac gcaacgacgt
//
LOCUS       DANJWC010000106         8480 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.106, whole genome shotgun sequence.
ACCESSION   DANJWC010000106 DANJWC010000000
VERSION     DANJWC010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8480
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.106"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            366..1394
                     /locus_tag="PWX02_001700"
     CDS             366..1394
                     /locus_tag="PWX02_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL1729694.1"
                     /translation="MKKTLSALAIAAATFTSTSTLAANEVNVYSYRQPYLIEPMLKNF
                     EKDTGIKVNIIFADKGLVDRVKQEGELSPADVLLTVDISRVMEIVNADLAQKIDSKVL
                     EKNIPAQFRDSNDQWFGLTTRARVIYTSKDRVGKLPAGFDYLDLAKPEYKGKVCVRSG
                     KNSYNVSLFAAMIEHYGIEKTKAFLEGLKANLAQKPQGGDRDQVKAIKEGICDYSIGN
                     SYYYGKMLDDEKQKSWAEAAIINFPSGEYGTHKNISGVVIAKHSPNKANAVKLIEYLS
                     GEKAQGLYAELNHEYPVKEGIEPSKLVKGWGTFTADTIKLEDIAKHYEAALKLVDEVK
                     FDDFSEKK"
     gene            1517..3163
                     /locus_tag="PWX02_001701"
     CDS             1517..3163
                     /locus_tag="PWX02_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL1729695.1"
                     /translation="MRSQFCWKSIAALTTFIVLLPLIAICYHALQGDNENLAHLWQTM
                     LPIYLKNSTLLVLGTVFLALIFALPTAWIVANYRFWGQRTLQWLLCLPLAMPAYLIAY
                     LYTDLLDYSGAFQGWLRSIFGWTHPQDYWFPQIRTLYGACFVLALVLYPYIFLLVRVA
                     LLEQSENLTHSAKMLGASPSQLFRRITLPLIRPSVAVGIALVAMETLGDFGTVAYFAV
                     PTLTTAIYDSWLGYHDLGTASQIAIFMLLMIFLFLSLEQYSRRKQKSYQRGYEKKSAF
                     KFLTGWKLALALAWCWGLLALAFFIPFGRLLYWAYDYFEQAWNLDFVQFSLNSLKVSI
                     IASVITVLIALLLHFSKRLNTRKILVKNDRLSLQLASLGYAVPGTVLAIGLLSPLTFA
                     DHQLHAWLKSLELSPVGLIFSGSLFALVLAYTIRFLAMAIGSLETSLNKISPSLDMAS
                     QTLGKNGSAMLVKIHIPLIYKGILTAGLMVFIESMKELNASLLLRPFNFDTLATYVFT
                     FTSDEQLERAALPAIVLVLVGLLPVIWLTRSLISQSEKER"
     gene            3173..4183
                     /locus_tag="PWX02_001702"
     CDS             3173..4183
                     /locus_tag="PWX02_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1729696.1"
                     /translation="MSVLTINHLNLNFGSTKVLQNLHLAVNQNEIICLLGASGCGKTT
                     LLKAIAGLLPIEQGDIHLAGECLKSKAVEERQIGLIFQDYALFPHLTVADNIQFGLSK
                     LPKVQQKAITEKMLSVVHLQGFESRFPHELSGGQQQRVAIARALACQPELLLLDEPFS
                     NIDSQTRYAMIQEIKQILKSQNVPAIFVTHSKEEAFAFADKIAVMDQGKIIQFGKPNE
                     LYHSPVNPFVADFLGGTNYLNCNVTEDNVLCSPIGEFRLFPEMNVQKGRYRWLLRPEQ
                     IILKPNELGLGKVVSKLFLGLYYRYQVEIKGIEIVTYQSQELKIGEQVEVGFQVREFI
                     LF"
     gene            4193..4819
                     /locus_tag="PWX02_001703"
     CDS             4193..4819
                     /locus_tag="PWX02_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HDL1729697.1"
                     /translation="MKIEVWSDYACPFCYIGKRHLEQALAQFEGEVEVVFRAFELDPH
                     ANGEPEGDIQQRLMRKYQKTAEQADEMIRYVEQAGKQAGLDLRYRTTQYTRTFEAHRL
                     AKFAKSKGLGEVMIERLFKAYFSDNLILAKRTQLIDLALELGLDRDEVAQLLTGDDFG
                     HEVREDERMAHRYGINSVPFFVIDEKLGVSGAQPPKVLLDAIKQALQK"
     gene            complement(4881..6371)
                     /locus_tag="PWX02_001704"
     CDS             complement(4881..6371)
                     /locus_tag="PWX02_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HDL1729698.1"
                     /translation="MSLYQIARTETGKVISLNAKMANRHGLIAGATGTGKTVTLRKLA
                     ESLSDDGVPVFLVDVKGDLSGLVQAGNFSGKIAERILQFNLGGEDYLKGYPVSFWDVF
                     GTSGIPLRTTISEMGPMLLSRLLNLNDTQGGLLNLVFRVADDRGLLLIDLKDLRALLK
                     FVADNAKEFQLEYGHISAASVGAIQRALLRLENEGADNLFGEPALDLHDWIQTRNGRG
                     VINMLNAEKLINSPRVYSAFLLWFMAELFENLPEVGDPEKPTFVLFFDEAHLLFDDAP
                     KALVDKIEQVVRLIRSKGVGVYFVTQNPLDLPDSVLGQLGNRIQHALRAFTPCDQKAV
                     KSAAETFRANKEVNVVEAITQLGVGQALVSVLDEKGMPTPVEIAYIYPPKSQLAPLAM
                     GERDAIVKQDELYHHYSQYVNNQSAFELLNAKAEAIKAEQAAAEEANNDFFGGILSSI
                     FGSKKKKDQSITEQVVSQVAHSVGRNLRNQVTKQIMRGILGAITKK"
     gene            complement(6430..7665)
                     /gene="rhlB"
                     /locus_tag="PWX02_001705"
     CDS             complement(6430..7665)
                     /gene="rhlB"
                     /locus_tag="PWX02_001705"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HDL1729699.1"
                     /translation="MSNHLTEHRFIDFPIHTNVLNALNSKGFEFCTPIQAKTFPITLN
                     GNDIAGQAQTGTGKTLAFLIATFNHLLNNEIETAKNQPRALILAPTRELAVQIGADAQ
                     LFLPHCDLKLALAYGGDGYDKQIQAIEKGVDILVGTTGRVIDYVKQGIIHLDKIQVIV
                     LDEADRMFDLGFIKDIRYLMRKAPVPTERLTLLFSATLSAKVRELAYEDMDNAEYIEI
                     EPLQRTGHRIKEELFYPSNEDKMALLLTLLEEEWPDRCMIFANTKHKCEEIWQYLAAD
                     GHRIGMLTGDIPQKKRLSLLDNFTKGNLDILVVTDVAARGLHIPDVTHVFNYDLPDDK
                     EDYVHRIGRTGRAGESGHSISFACERYAVNLPAIEEYIGHQIPVSQYDKESLLTLPKV
                     NRSRSKKTFLAKTKKNSRN"
     gene            complement(7742..8464)
                     /gene="recO"
                     /locus_tag="PWX02_001706"
     CDS             complement(7742..8464)
                     /gene="recO"
                     /locus_tag="PWX02_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDL1729700.1"
                     /translation="MTTDQWQRAFVLHRREYSESSLLVEFFTEHHGRITLLAKGARRA
                     RSPLKAVLQPFTPLLVRWSGKGDLKTLTKAEPAALTLPMETAALYSGFYINEVLARVL
                     ENQTAYPELFQHYLRCMTELATQHQIEPILRTFEFHTLKALGYGVDFTHCTATGLPID
                     PKMSYQFRESEGFIASLLQNNNSFLGCDLLAFAQLDFSEKQTQQAAKRFTRIALKPYL
                     GSQPLKSRELFQSILPNKVKSG"
BASE COUNT         2527 a         1772 c         1613 g         2568 t
ORIGIN      
        1 caaagttgtt tgtcttacca cttatgtaag gcaagacggg gtttagcctc cacatatcct
       61 agaaaatcga tctttttata aagacacccc aattcctagc ttgcaatatg tgtgtgtttt
      121 agtgttaaaa aataaatgtt tatgttttct atcttaacga agacaaaaaa cggcgtgtat
      181 tatgccaaaa aacaaacagc ttgccaaata ttttctccat tttgcgagct tatcatttta
      241 agaaatgcta aaagccatca tttaaaaaat caataacaaa aaccgttctc attattgact
      301 aaaaaaatgg ggaggtttag aatgtcttcc attctgttat gaaaacctgt ctgaaggaga
      361 ctatcatgaa aaaaacactt tctgcacttg ctattgctgc agccactttt acatcaactt
      421 caacgcttgc tgcaaatgaa gtgaatgttt attcttaccg ccagccttac ttaattgagc
      481 caatgctaaa aaactttgaa aaagatactg gcattaaagt aaacatcatc ttcgctgata
      541 aagggttggt tgatcgtgta aaacaagaag gtgaattaag tcctgctgat gtgcttttaa
      601 cagtagatat ttcacgcgta atggaaattg ttaacgctga tcttgcacaa aaaattgact
      661 ctaaagtgtt agaaaaaaat attcctgcac aattccgtga ctctaacgac caatggtttg
      721 gtttaaccac acgtgctcgt gttatctaca cgtcaaaaga tcgtgtaggt aaattaccag
      781 caggctttga ctacctagat ttagctaaac cagagtataa aggtaaagtc tgcgtgcgtt
      841 caggcaaaaa ctcttacaat gtctcactat tcgcggcgat gatcgaacat tacggcattg
      901 aaaaaaccaa agcattctta gaaggcttaa aagcgaactt agcacaaaaa ccacaaggtg
      961 gtgaccgtga tcaagtaaaa gcaattaaag aaggcatctg tgattactct atcggtaaca
     1021 gctactacta cggcaaaatg ttagatgatg agaaacaaaa atcgtgggct gaagcagcga
     1081 ttattaattt cccgagcggt gaatacggca cgcacaaaaa catcagcggc gttgttattg
     1141 caaaacactc accaaacaaa gcgaatgcag tgaaattaat tgagtacctc agcggcgaaa
     1201 aagcccaagg tttatatgcg gaacttaacc acgaatatcc ggtaaaagaa ggcattgagc
     1261 cgtctaagct ggtgaaaggc tggggaacat ttaccgctga taccatcaaa ttagaagaca
     1321 tcgccaaaca ctacgaagcg gcattaaaat tggtcgatga agtgaaattc gacgatttct
     1381 ctgagaaaaa ataatcaacc gataagcggt catttttcca caaaaatttg caaaattttg
     1441 ttaaaataca accgcttgca aaaggacgca ttttgcgtcc ttttattttt ttatgaaata
     1501 acaggcttaa agaaaaatgc gatcacaatt ttgctggaaa agtattgctg ccctaacaac
     1561 ttttatcgtt ttgcttcctc ttattgcaat ttgctatcac gcattgcaag gggataatga
     1621 aaaccttgct cacctttggc aaacaatgtt gccaatctat cttaaaaact caactttatt
     1681 agtattagga accgttttcc ttgcactgat ttttgctctg cctaccgctt ggattgtggc
     1741 aaattaccgt ttttgggggc aaagaacctt acaatggtta ctttgtttgc ctctcgctat
     1801 gcctgcttat ttaattgcct atttatatac cgatttatta gattactcag gtgcttttca
     1861 aggctggctg cgtagtattt ttggctggac tcatccgcaa gattactggt ttcctcaaat
     1921 tcgtacattg tatggtgctt gctttgtgct tgcgttagta ctatatccat acattttttt
     1981 attagtgcga gttgccttat tagaacaatc tgaaaactta acacacagtg caaaaatgct
     2041 cggggcttcg ccctctcagt tgttccgtcg aattacgctg ccattaattc gaccttctgt
     2101 tgccgtaggc attgcattag tggcaatgga aacgcttggc gatttcggta ctgtggccta
     2161 ttttgccgtg ccaacactta ctacggcaat ttatgattcg tggctaggct atcacgatct
     2221 tggtactgcc agccagattg cgatctttat gttgctaatg attttcctgt ttttaagttt
     2281 agagcaatat agtcgccgta aacaaaaaag ctatcaacgt ggctatgaaa agaaatcagc
     2341 ctttaaattt ttaactggtt ggaaattagc attagccctt gcttggtgct ggggtttatt
     2401 ggcgttagcc tttttcattc cgtttggcag gttgctctat tgggcatacg actatttcga
     2461 gcaagcgtgg aatttagact ttgtccaatt ttcgcttaat agtttgaaag tatcgatcat
     2521 cgcctccgtg ataacggtac tgattgctct acttctgcat ttttctaaac gtttgaatac
     2581 ccgcaaaatt ttagtcaaaa atgaccgctt gtcattacaa cttgcctcac tgggctatgc
     2641 ggttcctggc acagtattag cgatcggttt actttcgcct ttaacatttg ccgatcatca
     2701 actccatgct tggctaaaaa gccttgaatt atcacccgtt ggattgattt tttcaggctc
     2761 actctttgca ttagtattag cttataccat ccgattttta gcaatggcaa ttggcagcct
     2821 tgaaacttcg ctaaataaaa tctctccatc gctggatatg gcgagccaaa cgcttggtaa
     2881 aaatggctca gcaatgttag taaaaattca tatcccttta atttataaag gtattttaac
     2941 tgcaggatta atggtcttta ttgagagtat gaaagagcta aatgcttctc tgctacttcg
     3001 cccatttaat tttgatacac tcgcaaccta tgtgtttacc tttacatcag acgagcagct
     3061 tgagcgtgct gctctgcctg ccatcgtatt ggtgttagtc ggtttactgc ctgttatttg
     3121 gcttaccaga tcccttattt cgcaatctga aaaagaaaga taaaggaatt ttatgtctgt
     3181 tttaactatt aatcatctca acctcaattt cggcagtaca aaggttttac aaaatctcca
     3241 tcttgctgtg aatcaaaatg aaattatctg tttgctaggt gcaagtggct gtgggaaaac
     3301 tacactgctc aaagccattg caggtttatt gccaattgag cagggcgata ttcatttagc
     3361 gggtgaatgc ctaaaaagca aagccgtgga agagcgacaa atcgggctga ttttccaaga
     3421 ttacgctctt tttccacact tgacggtagc cgataatatt caatttggct tatccaaatt
     3481 gccaaaagta caacaaaaag caattaccga aaaaatgctt tcagttgtgc atttacaagg
     3541 ctttgaaagc cgttttccac acgaactttc gggcgggcaa caacagcgtg tcgctattgc
     3601 ccgagcatta gcctgccagc cagaattgct actgttagat gagccgtttt ccaatattga
     3661 cagccaaact cgctatgcaa tgattcaaga gatcaagcaa atcttaaaaa gccaaaatgt
     3721 gcccgctatt tttgtaaccc atagcaaaga agaagccttt gcatttgccg ataaaattgc
     3781 cgtgatggat caagggaaaa ttattcaatt tggtaagcca aacgagctat accattctcc
     3841 agttaatcca tttgtggcag attttttagg tggcacaaac tatcttaact gtaatgtaac
     3901 ggaagacaac gtgttatgca gccctattgg cgagttccga ctcttccctg aaatgaacgt
     3961 tcagaaaggg cgttaccgtt ggcttcttcg cccagaacag attatcttaa aacccaatga
     4021 gctaggttta ggtaaagtgg taagcaaact gtttttaggc ctatactacc gctatcaggt
     4081 tgaaattaaa ggcattgaga tagtaactta ccaatctcaa gagctgaaaa ttggcgaaca
     4141 agtggaagtg ggcttccaag taagagagtt tatcctattt taaggagaat atatgaaaat
     4201 tgaagtttgg tcagattatg cctgcccgtt ttgttacatc ggcaaacgcc acttagagca
     4261 agccctagcc caatttgaag gcgaggttga agtggttttc cgagcctttg agcttgatcc
     4321 gcacgctaac ggcgaaccag agggcgatat tcagcaacgc ctaatgcgga aatatcaaaa
     4381 aaccgcagaa caagctgatg aaatgattcg ttatgtggaa caagccggca aacaagctgg
     4441 gctagatttg cgttaccgca ccacccaata cactcgcacc tttgaagctc atcgcttggc
     4501 gaaatttgcg aaaagcaaag gtttgggcga agtgatgatt gaacgtttat tcaaagccta
     4561 tttttccgat aatctcattt tggcgaaacg cactcaactt atcgacttag cgttagagct
     4621 cggtttagat cgtgatgaag tggctcaatt acttacaggt gatgatttcg gccacgaagt
     4681 acgggaagat gaacgtatgg ctcaccgcta tggcattaat agcgtgccat tttttgtgat
     4741 tgatgaaaaa ctcggtgtgt caggagctca accgccaaag gtgttgctag atgctatcaa
     4801 acaggcattg caaaaataat ccgaaaaaag accgcttgtt aagctaaaca agcagtctta
     4861 ttcttcttat aatttacaat ttattttttg gtgatcgcac ctaaaatacc acgcataatt
     4921 tgtttggtga cttggttgcg taagtttctg ccaactgaat gcgccacttg gctaaccact
     4981 tgttcggtaa tcgattgatc ttttttcttc ttcgagccaa aaatggaaga gagaataccg
     5041 ccaaagaagt cattgtttgc ttcttctgcc gcagcctgct ctgctttaat tgcttctgct
     5101 ttcgcattga gtaattcaaa ggcagattga ttattcacat attggctgta gtgatgatat
     5161 aactcatctt gtttcactat agcatcacgc tcgcccatcg ctaatggtgc taattggctt
     5221 tttggcggat aaatataggc aatttctaca ggcgttggca tacctttttc atctagcacc
     5281 gaaaccaacg cttgccccac accaagttgc gtgatggctt ctaccacatt tacctcttta
     5341 ttggctcgga aagtttccgc tgctgatttt accgcttttt gatcacatgg cgtgaaagca
     5401 cgcaaagcgt gttggatacg attgccaagc tgccctaata ctgaatcagg taagtcgagt
     5461 ggattttgcg taacaaaata aacacccacc ccttttgagc gaattaaacg aaccacctgt
     5521 tcaattttat ccaccaatgc cttaggggca tcgtcaaaca ataaatgagc ttcatcaaag
     5581 aataaaacaa acgtcggttt ttctgggtcg ccaacttctg gcaaattctc aaagagctct
     5641 gccataaacc aaagcaggaa tgcactgtac actcttggtg agttaattaa tttttctgca
     5701 ttcaacatat tgattacacc acgcccatta cgggtttgga tccaatcgtg caaatctaat
     5761 gcaggctcac caaataaatt atctgcccct tcattttcaa gccttaataa cgcacgctga
     5821 atagctccaa cgcttgctgc agaaatatgc ccatattcca gttggaattc tttcgcatta
     5881 tctgcgacaa atttaagcaa cgcacgcaaa tcttttaaat caattagcaa taaacctctg
     5941 tcatctgcca cacgaaaaac gagatttaac aagcctcctt gggtatcgtt taaattgagt
     6001 aaacgagaaa gtaacatcgg tcccatttcg gaaatagtgg ttcgaagtgg gatacctgat
     6061 gtaccaaata cgtcccaaaa cgaaactgga tagcccttta agtaatcttc tccccctaaa
     6121 ttaaactgta aaatgcgttc tgcaattttt cctgaaaaat tacccgcttg taccaagccc
     6181 gaaagatcac cttttacatc tactaaaaat actggcacac catcatcact caaagattct
     6241 gccaatttgc gtaaagtaac cgttttacct gtacccgtag ccccagcaat taaaccgtgc
     6301 cgatttgcca ttttggcatt aagcgaaata actttgcctg tttctgttcg agcaatttga
     6361 tacaaactca tctttcattc tcttaaaggt aaattaattt tttgttctca tcatataaaa
     6421 atttatcact taattacggc tattcttttt cgtttttgcc aagaacgttt ttttacttct
     6481 tgagcggttc actttcggta aagtcagtaa ggattccttg tcgtattgac tcacaggaat
     6541 ctgatgccca atatactctt caattgcagg caaattaacc gcataacgct cgcaagcaaa
     6601 actaattgaa tggccgctct cccctgcccg acccgttcta ccaatacgat gaacgtaatc
     6661 ctctttatca tcaggtaaat cataattaaa cacatgcgta acatctggaa tatgtaatcc
     6721 acgtgctgcc acatcagtca cgactaaaat atctaaatta cctttggtaa aattatctaa
     6781 cagagatagg cgttttttct gtggaatatc acctgttagc attccaatcc gatgaccatc
     6841 ggctgctaaa tattgccaaa tttcctcaca tttatgctta gtattggcaa aaatcataca
     6901 gcggtctggc cattcttcct ccaataacgt caataaaagt gccattttgt cttcattaga
     6961 cggataaaat aactcttctt ttatacgatg ccctgtacgt tgtagcggtt caatctcaat
     7021 atattcagca ttatccatat cttcataagc aagctcacgt actttagcgg ataaagttgc
     7081 tgaaaagagc aacgttaaac gctcagtagg cacgggagct tttctcatta aataacgaat
     7141 atctttaata aaaccgagat caaacatacg atcagcttca tctagcacaa tcacttggat
     7201 tttatctaaa tgaataatcc cttgcttaac ataatcgatc acacgccctg tggttccaac
     7261 taaaatatct accccttttt ctatggcttg tatttgcttg tcgtaaccat caccaccata
     7321 agcaagggca agtttaagat cgcaatgtgg taaaaaaagc tgtgcatctg ccccgatttg
     7381 taccgccagc tcacgagttg gtgcaagaat taatgctctt ggctgatttt ttgctgtctc
     7441 aatttcgtta tttaataaat ggttgaacgt ggcaattaaa aaagcaagcg ttttacctgt
     7501 gcctgtttga gcctgtcctg caatatcatt tccgttaagc gtaatcggga aagtttttgc
     7561 ttggataggt gtacagaact cgaagccttt actattcaac gcattgagta catttgtgtg
     7621 aatggggaag tcaataaaac ggtgttcggt aagatgattt gacataatat ttccttgaga
     7681 tatttgttat tgctacaagc ggttatattt taattaaaat ttgcaaaaaa atttaacaaa
     7741 actaaccgct tttcacttta ttaggcaata tagattgaaa aagttctcgg ctttttagtg
     7801 gctgagaacc taaataaggt ttcaatgcaa ttcgggtaaa tcgtttggca gcttgttgtg
     7861 tttgtttttc tgaaaaatct aattgagcaa acgccaataa atcacagcct aaaaagctat
     7921 tgttattttg taataatgag gcaataaagc cttcactttc tctaaattgg taactcattt
     7981 tcggatcaat tggcaaacct gttgccgtac aatgggtaaa atctacgcca tagcctaatg
     8041 cttttaatgt atgaaattca aaagtacgca aaatcggctc aatttggtgt tgagtagcca
     8101 gctccgtcat acaacgcaaa taatgttgaa ataattcagg gtaagcggtc tgattttcta
     8161 acactcttgc aagcacctca ttgatataaa aaccgctata aagtgcagcc gtttccattg
     8221 gtaaagtaag agcagcaggc tctgcctttg tgagtgtttt taaatcaccc ttgccagacc
     8281 aacgcactaa caagggtgta aaaggctgaa gcacggcttt caacggcgag cgagcccgcc
     8341 ttgcaccttt agcaagtaaa gtaatccgcc cgtgatgttc ggtaaaaaac tccactaata
     8401 aactgctttc gctgtattca cgccgatgaa ggacaaaggc tcgttgccat tgatctgtgg
     8461 tcataattaa tcccgactat
//
LOCUS       DANJWC010000107         8411 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.107, whole genome shotgun sequence.
ACCESSION   DANJWC010000107 DANJWC010000000
VERSION     DANJWC010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8411
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.107"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..204)
                     /locus_tag="PWX02_001707"
     CDS             complement(<1..204)
                     /locus_tag="PWX02_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HDL1729701.1"
                     /translation="MNRDIRWKQRFDNYQKSVSYLQAEAEKYADTDIDVIKKGIIQSF
                     EITHELAWKLMQDILKWEGEVDIY"
     gene            complement(206..451)
                     /gene="nqrM"
                     /locus_tag="PWX02_001708"
     CDS             complement(206..451)
                     /gene="nqrM"
                     /locus_tag="PWX02_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HDL1729702.1"
                     /translation="METVLLTFGFFVAVIFAMSIGFIVKGKTIKGSCGGITALGMKKM
                     CDCEEPCDNLKSKIADGTADPEEVARFNKEPQFYEVK"
     gene            complement(464..1498)
                     /locus_tag="PWX02_001709"
     CDS             complement(464..1498)
                     /locus_tag="PWX02_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDL1729703.1"
                     /translation="MKKFFLLLIAFFTLSACEKAPEQITLSGKTMGTTYTVKYIDNGE
                     VENLISPEKMKNELDGLLIEVNNQMSTYQQDSEISRFNQAKQANQAVEISQDFAKVVQ
                     EAIRLNKVTEGALDVTVGPLVNLWGFGPDKRLNKVPSDEQIAERSAYVGIDKILLEMS
                     SKPKLTKSAANLYLDLSSIAKGFGVDKLAEHLETIGVSNYLVEIGGELRGKGKNLQGL
                     DWRIAIEQPVMTQGQAAQITVPLHNLAMATSGNYRNYFEDEKGNRLSHIIDPKQLRPV
                     NHNLASITVFAPTTMTADGLSTGLFVLGAEKALAVAEREKLAIFLIVKNGDKFETQMS
                     SEFSKLIQSQ"
     gene            complement(1547..1840)
                     /locus_tag="PWX02_001710"
     CDS             complement(1547..1840)
                     /locus_tag="PWX02_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL1729704.1"
                     /translation="MIDLTESQRQQVLDIFKKYLPDTPVWVFGSRIKGTAKAYSDLDL
                     ALITKQPLTIRQQTELELAFTDSDLPFTVDLIDWASCNESFKQIILQRYEVLI"
     gene            complement(1833..2267)
                     /locus_tag="PWX02_001711"
     CDS             complement(1833..2267)
                     /locus_tag="PWX02_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HDL1729705.1"
                     /translation="MEQLDITALENAFNSLEKTIVSLENQAWFNRQEAIIQDTLIAGA
                     IQKFEFVYELSIKMLKRQLKNIESNDTDIDSTEFRDILRMAAQYGLIESPEVWLDYRK
                     MRNITSHTYDQNKAQQVYNGISNFLESTYFLVKQLQKRNSYD"
     gene            complement(2379..3608)
                     /gene="nqrF"
                     /locus_tag="PWX02_001712"
     CDS             complement(2379..3608)
                     /gene="nqrF"
                     /locus_tag="PWX02_001712"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HDL1729706.1"
                     /translation="MDSNFIFGIIAFTALVLVLAVIILFAKSKLVDSGDITISINNDP
                     EKGITLPAGGKLLGALASKGIFVSSACGGGGSCGQCKVQVKSGGGEILPTELSHISKK
                     EAKEGWRLACQVNVKSSMDVELPEEIFGVKKWECTVISNDNKATFIKELKLQIPEGEE
                     VPFRAGGYIQIEAEPHTVHYKDFDIPKEYHEDWDKFDLWRYTSKVDEHIIRAYSMASY
                     PEEKGIIMLNVRIATPPPRNPDVPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAE
                     MVFIGGGAGMAPMRSHIFDQLKRLKSKRKMSFWYGARSEREMFYVEDFDGLQAENDNF
                     KWYVALSDPLPGDREDYFRGFIHNVLYENYLKNHEAPEDCEYYMCGPPVMNAAVIGML
                     KSLGVEDENILLDDFGG"
     gene            complement(3619..4215)
                     /gene="nqrE"
                     /locus_tag="PWX02_001713"
     CDS             complement(3619..4215)
                     /gene="nqrE"
                     /locus_tag="PWX02_001713"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005529392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HDL1729707.1"
                     /translation="MEHLLSIFVKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGIA
                     VIVVLGIAVPANQLVYTHVLKEGALVEGVDLSFLNFITFIGVIAALVQILEMILDKFF
                     PALYSALGIFLPLITVNCAIFGGVSFMVQREYNFVESVVYGVGAGTGWMLAIVALAGL
                     TEKMKYSDVPAGLRGLGITFITVGLMALGFMSFSGIQL"
     gene            complement(4217..4843)
                     /locus_tag="PWX02_001714"
     CDS             complement(4217..4843)
                     /locus_tag="PWX02_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005717817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HDL1729708.1"
                     /translation="MADNNNLKKLLLSPIVDNNPIALQILGICSALAVTTQLQTAVVM
                     AIAVSLVTAFSSMFISMIRNYIPNSIRIIVQMAIIASLVILVDQILRAYAYDLSKQLS
                     VFVGLIITNCIVMGRAEAFAMKSGPVESFVDGIGNGLGYGAMLIIVAFLRELIGSGKI
                     FGITILETVQNGGWYQANGLFLLAPSAFFIIGFVIWAIRTWKPEQVEK"
     gene            complement(4843..5616)
                     /locus_tag="PWX02_001715"
     CDS             complement(4843..5616)
                     /locus_tag="PWX02_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HDL1729709.1"
                     /translation="MAKFNKDSVGGTLTVVVLLSLICSLIVAGAAVMLKPTQDIQKQL
                     DKQKNILQAAGLLQKDTNVQETYTKFIEPKIVDLATGDYVEGVTGFDARAAAKDPAQN
                     VVIAPEDDKANIKTRAKYAEVYLVKDESGKTTQVVLPMHGNGLWSIMYGFVAVQPDAN
                     TVNGITYYEQGETAGLGGEIVNPNWQKNFVGKKLYNEQNQVALRVGKGASADKDHGVD
                     GLSGATLTSNGVDGSFKYWFGQNGFGPYLAKFKAAAGAN"
     gene            complement(5609..6844)
                     /locus_tag="PWX02_001716"
     CDS             complement(5609..6844)
                     /locus_tag="PWX02_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HDL1729710.1"
                     /translation="MGLKNLFEKMEPAFHKGGKYEKWYTLFEAAYTIFYTPGTVTRKD
                     AHVRDAIDSKRMMLIVWLALFPAMFYGMYNVGAQGILAALKMGTLTDLVANNWHYSLA
                     DSLGSVADAGWGTKMLLGATYFLPIYLTVFAVGGFWEVVFAMVRKHEINEGFFVTSIL
                     LALIVPPTLPLWQAALATTFGVVVAKEVFGGVGKNFMNPALAGRAFLFFAYPGQISGD
                     AVWVAADGFSGATALSQWAVGGQGALQHVATGETITWMDAFLGNIPGSIGETSTLMLI
                     IGAAIIVFARIASWRIIAGVMIGMAATATLFNVIGSDTNPLFSMPWHWHLVLGGFAIG
                     MFFMATDPVSAAFTNKGKWAYGILIGFMCVLIRVVNPAYPEGMMLAILFANLFAPIFD
                     YLVVQGNIKRRLARVANNG"
     gene            complement(6847..8205)
                     /locus_tag="PWX02_001717"
     CDS             complement(6847..8205)
                     /locus_tag="PWX02_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HDL1729711.1"
                     /translation="MITIKKGLDLPIAGKPEQVIHNGNTVTEVAMLGEEYVGMRPSMK
                     VREGDVVKKGQVLFEDKKNPGVVFTAPASGTVTAINRGEKRVLQSVVIKVEGDEQITF
                     ARYEASQLASLTSEQVKQNLVESGLWTAFRTRPFSKVPALDAEPSSIFVNAMDTNPLA
                     ANPEVIIAEHQQAFFDGLTVLSRLFESKKVIHLCGSADSNILNAAQSRQINNVETNRF
                     KGPHPAGLSGTHIHFIDPVGATKQVWYLNYQDVIAIGKLFTTGELFTDRVVALAGPQV
                     KNPRLVRTRLGANLSQLTANELNAGENRVISGSVLSGAIATGVHDYLGRYALQVSALL
                     EGREKAFFGMIAPYSDKYSITRTTLGHFAKKLFNFTTAVHGSERAMVPTGAYERVMPL
                     DIIPTLLLRDLAAGDTDSAQALGCLELDEEDLALCTFVCSGKNNWGPLLRQALDKIEK
                     DG"
BASE COUNT         2524 a         1887 c         1493 g         2507 t
ORIGIN      
        1 ataaatatca acttcacctt cccattttaa aatatcttgc attaacttcc acgctaactc
       61 gtgtgtaatc tcaaaactct ggataattcc ttttttaatt acatcaatgt cggtatcagc
      121 gtatttttct gcttctgctt gaaggtaact tactgatttt tgatagttat caaaccgctg
      181 tttccagcga atatctctat tcatattatt tcacttcata aaattgtggc tctttattaa
      241 aacgtgccac ttcttcaggg tcggctgtgc cgtctgcgat tttagatttt aggttatcgc
      301 aaggttcttc gcagtcgcac atttttttca tacctaaagc ggtgataccg ccacagcttc
      361 ctttgatggt tttgcctttc acgataaaac caatcgacat cgcaaaaatt acggctacaa
      421 aaaagccaaa ggttaataat acagtttcca tttttcactc caactattgt gattgaataa
      481 gtttgctaaa ttcgctcgac atttgtgtct caaatttatc gccgttttta acaattaaaa
      541 aaatagctaa tttttcacgc tctgccactg ctaatgcttt ttccgctcct aaaacaaata
      601 aaccggttga taagccgtct gccgtcattg tagttggggc aaatacggtg atagaggcta
      661 aattatggtt taccggacgt aactgttttg ggtcgataat atgtgaaaga cggttgcctt
      721 tttcatcttc aaaataatta cgataattgc ccgaagttgc catcgccaaa ttgtgcaacg
      781 gcacggtaat ttgtgcggct tgaccttgtg tcattaccgg ctgctcgatt gcaatccgcc
      841 aatctaagcc ttgtaaattt tttcctttgc ctcgtaattc gccaccaatt tcaactaaat
      901 agttgctcac acctatagtt tcaagatgct cggctaattt atctacaccg aaaccctttg
      961 caatagacga taaatctaga taaagattag ctgctgactt ggtgagcttg ggtttactac
     1021 tcatttcaag caaaatttta tcaatgccga cataagcaga acgctctgca atttgttcat
     1081 cagacggcac tttatttaag cgtttatccg gcccgaagcc ccacaagttc accaatggac
     1141 caacggttac atctaaagcc ccttcggtta ctttatttaa acggattgcc tcttgtacca
     1201 cttttgcaaa atcctgcgaa atttcgaccg cttgatttgc ctgctttgct tgattaaaac
     1261 ggctaatttc cgaatcttgt tggtaggtgg acatctgatt attcacctca atcaataagc
     1321 catccagctc atttttcatt ttttcaggcg aaatcaaatt ctccacttca ccattatcaa
     1381 tatattttac ggtgtaagtc gttcccatgg ttttgcctga cagcgtaatt tgctcagggg
     1441 ctttttcaca agcacttaaa gtaaaaaaag caattaaaag aagaaaaaat tttttcattg
     1501 gtgtcacaaa agttaaaaat tggggaaatt atagcatatt tagcgattaa ataagcactt
     1561 catatcgctg taaaataatt tgtttaaacg attcgttgca acttgcccag tcaatcagat
     1621 ctactgtgaa aggtaggtca gaatccgtaa acgcaagctc taactctgtt tgttgccgaa
     1681 ttgtgagcgg ttgtttggtg attaatgcta aatcaagatc cgaataagct ttggctgtgc
     1741 ctttaatgcg agagccaaaa acccaaacgg gtgtatcagg caagtatttt ttgaaaatat
     1801 ccaacacctg ttggcgttgt gactcagtta aatcaatcat agctattgcg tttttggagc
     1861 tgtttaacta aaaaatacgt actttccaga aagtttgaaa ttccgttata aacctgctgt
     1921 gctttatttt gatcataagt atgcgaagta atatttcgca ttttgcgata atccagccaa
     1981 acttccggtg attcaatcag cccatattga gcagccattc gcaaaatatc acggaactcg
     2041 gtgctatcaa tatcagtgtc atttgactca atgtttttga gctgacgctt gagcattttt
     2101 atcgacaatt cgtaaacaaa ttcaaatttt tggattgctc ctgcaatcaa agtgtcttga
     2161 ataattgctt cttgccggtt aaaccacgct tgattctcaa ggctgacaat cgttttctca
     2221 agggaattga aggcattttc aagtgccgta atatccagtt gttccatttg cttcccctta
     2281 aaattcacat tattaaatta ccaaaactta ataatataaa ttaaatcaag cggtcaattt
     2341 tgcaaatttg accgcttgta gtcagaagtt aaggtttatc aaccaccaaa gtcatctaat
     2401 aagatgtttt cgtcttctac gcctaaactc ttcagcatac cgattaccgc agcattcatc
     2461 accggtggac cacacatata gtattcgcag tcttcaggtg cttcgtggtt tttgaggtag
     2521 ttctcataaa gcacgttatg aataaagcca cggaagtaat cttctctgtc gcccggtaat
     2581 ggatcagaaa gtgccacata ccatttgaag ttatcgtttt ctgcttgaag accatcaaaa
     2641 tcttccacat agaacatttc acgctcagaa cgagcaccat accagaatga cattttacgt
     2701 ttagatttta aacgttttaa ttggtcgaag atatgtgaac gcattggtgc catacctgca
     2761 ccaccaccga taaagaccat ttccgcatcg gtatctttag cgaagaactc accgaatgga
     2821 cctgaaattg ttactttatc acccggtttt aatgaccaaa tgtaggaaga catttgaccc
     2881 ggcggtacgt ccggattgcg tggtggaggt gtagcaatac gcacgttaag cataataatg
     2941 cctttctctt ccggatatga agccattgag tatgcacgaa taatatgctc gtccactttt
     3001 gaagtataac gccataagtc aaatttatcc cagtcttcgt ggtactcttt tggaatatcg
     3061 aagtctttat aatgaacggt atgaggttca gcttcaattt ggatataacc acccgcacgg
     3121 aacggtactt cttcgccttc aggaatttga agtttaagct ctttgatgaa ggttgctttg
     3181 ttatcgtttg agataacggt acattcccat tttttcacac caaagatctc ttccggaagt
     3241 tcaacgtcca ttgaagattt cacgtttacc tgacaagcta aacgccagcc ttctttcgct
     3301 tctttcttag aaatgtgcga taactcagtt ggaagaattt caccaccgcc tgatttcact
     3361 tgtactttac attgaccgca agagccaccg cccccacaag ctgacgaaac gaaaatccct
     3421 ttacttgcta aagcaccgag taattttcca ccagccggta gcgtaatgcc tttttccgga
     3481 tcgttgttga ttgagatggt aatatcacct gaatcaacta attttgattt agcgaaaaga
     3541 atgatcaccg caagcactaa taccagagcc gtaaatgcga taataccgaa aataaaatta
     3601 ctatccacga tatgctcctt ataattgaat gcctgagaat gacataaagc ctaacgccat
     3661 taagcctacc gtaataaagg taatacctaa accacgtaag cctgccggca cgtcggaata
     3721 tttcattttc tcggttaaac ctgcaagggc aacgatggct aacatccagc ctgtacctgc
     3781 acctacgcca taaaccacag attctacaaa gttatactca cgttgtacca taaacgatac
     3841 accaccaaaa atcgcacagt ttacggtgat aagtggtaag aagataccta atgcactata
     3901 aagtgccggg aaaaacttgt ctaaaatcat ttccagaatt tgaacaagtg ccgcaataac
     3961 cccgatgaaa gtgataaagt ttaagaagct taaatccaca ccttctacta atgcaccctc
     4021 ttttaatacg tgggtataaa ctaattggtt agctggaact gcaatgccaa gcactacaat
     4081 taccgcaata cctaaaccaa aagcagttga aactttctta gacaccgcaa ggaatgtaca
     4141 cataccaagg aagaaagaaa gtgccatatt ttcaataaat acggacttaa caaagatact
     4201 cagaagatgt tccatcttat ttctccactt gttccggttt ccacgttcta atcgcccaga
     4261 tcacaaatcc gataataaag aacgcacttg gtgctaataa gaataaaccg tttgcttgat
     4321 accaaccgcc attttgaaca gtttcaagaa tggtgatacc gaaaatttta cctgaaccga
     4381 taagttcacg taaaaaggca acgataatta acattgcacc gtagcctaaa ccgttaccga
     4441 taccatcaac aaaactttca accgggcctg atttcatcgc gaatgcttct gcacgcccca
     4501 ttacgataca gttggtaata ataaggccta cgaataccga taattgtttt gacagatcgt
     4561 aagcataagc gcgtaagatt tggtcaacca aaattactaa tgatgcaata atcgccattt
     4621 gcacaatgat acggatacta tttggaatat agttacgaat cattgaaatg aacatacttg
     4681 agaatgcagt aactaaactt accgcaatcg ccattactac tgctgtttgt aattgagtcg
     4741 ttaccgctaa cgcagaacaa atacctaaga tctgtaatgc gatcgggttg ttatcgacaa
     4801 ttggcgataa caataatttt ttaagattgt tattgtctgc cattagttag ctcctgccgc
     4861 tgctttaaat tttgctaaat atggaccaaa accattttga ccaaaccagt atttgaatga
     4921 accatcaaca ccgttcgagg ttaatgttgc accagataaa ccatcaacac cgtgatcttt
     4981 atctgcagat gcccctttac ccactcttaa cgcaacttgg ttttgctcgt tatacagttt
     5041 tttacctaca aagtttttct gccagttcgg gtttacaatt tcgccaccta agcctgccgt
     5101 ttcaccttgt tcatagtaag taataccgtt aacggtattt gcatcaggtt gtaccgcaac
     5161 gaaaccgtac ataattgacc ataagccgtt accgtgcatt ggtaatacta cttgagtcgt
     5221 tttgccagat tcatctttta ctaaataaac ttcagcatat ttagcacgcg ttttgatatt
     5281 cgccttatca tcttcaggtg cgataactac attttgagca ggatctttag ctgctgcacg
     5341 agcatcaaaa ccggttacac cctcaacgta atcacctgtt gctaaatcga caattttcgg
     5401 ctcaataaat ttggtatagg tttcttgcac attggtatct ttttgcaata aaccggccgc
     5461 ttgtaagatg tttttctgtt tatcaagttg tttctgaata tcttgtgttg gtttaagcat
     5521 tactgccgca cctgccacaa tcaaagaaca aattaaactt aataaaacta caacggttag
     5581 cgttccgcca acgctatctt tattaaattt agccattgtt tgctaccctc gctaatctgc
     5641 gtttgatatt cccttgaact actaaatagt caaagattgg agcaaataag ttagcaaata
     5701 agatcgctaa catcatacct tcagggtatg ctgggtttac cacacggatt aatacacaca
     5761 taaagccgat taagataccg tatgcccatt tacctttgtt ggtaaatgca gctgataccg
     5821 ggtcggttgc cataaagaac ataccgatcg caaaaccgcc taatactaaa tgccaatgcc
     5881 aaggcataga gaataatggg ttggtatcag aaccgattac attaaataat gtcgcagttg
     5941 ccgccatacc aatcattacg ccggcaataa ttcgccaaga agcaattcgg gcaaatacaa
     6001 tgattgcagc accaataatc aacattaagg tagaggtctc gccgatagaa cccggaatat
     6061 tacctaagaa cgcatccatc caagtaatgg tttcaccggt tgcaacgtgc tgtaatgcac
     6121 cttgaccacc aactgcccat tgagaaagtg cagttgcacc tgagaagccg tctgcggcaa
     6181 cccatactgc atcacctgaa atttgacccg ggtaagcaaa gaacaagaaa gcacgaccgg
     6241 ctaacgcagg gttcataaag tttttaccca cgccaccgaa cacttctttt gcgaccacaa
     6301 caccaaaggt agttgcaagg gctgcttgcc ataacggtaa tgttggagga acaattaatg
     6361 ctagtaagat agaggttaca aagaaacctt cgttgatctc gtgcttacgc accattgcaa
     6421 acaccacttc ccagaaacca cccactgcaa atactgttaa gtagattggt aggaaatagg
     6481 ttgcccccag caacatttta gttccccagc ctgcatcagc tacagagcct aaactatcgg
     6541 ctaagctgta gtgccagttg ttagcaacta aatccgttaa tgttcccatt ttgagtgccg
     6601 ctagaatacc ctgtgcacca acattgtaca ttccgtagaa catcgcaggg aataacgcta
     6661 accatacaat taacatcata cgcttagagt caatcgcatc acgcacgtga gcatctttac
     6721 gagtgaccgt acccggtgta tagaaaatcg tataggcagc ttcaaatagc gtgtaccatt
     6781 tctcatactt tccaccttta tggaaagcgg gttccatttt ttcaaaaaga tttttcaaac
     6841 ccatttttaa ccgtccttct cgatcttatc taacgcttga cgtaataatg gtccccagtt
     6901 gtttttaccg gaacacacaa aagtacataa cgctaaatct tcttcgtcta actctaagca
     6961 gcctaaagct tgagcggagt ctgtatctcc agctgctaaa tcacgcaata agagtgttgg
     7021 aatgatatcc aacggcatta cacgctcata agcccctgtt ggtaccattg cacgctcact
     7081 accgtgaact gctgttgtga agttaaataa tttcttcgca aaatgcccta aagtagtacg
     7141 cgtaattgaa tacttatcag agtaaggagc aatcataccg aagaacgctt tctcacgccc
     7201 ttctaataaa gcagacactt gtaatgcgta acgacctaaa tagtcgtgta ctcccgttgc
     7261 gatagcacca cttaacacag aacctgaaat gacacggttt tcacccgcat ttaattcgtt
     7321 ggctgttaat tgtgaaagat tcgcccctaa acgagtacgc accaaacgtg gatttttcac
     7381 ttgtggcccg gctaatgcga ctacacggtc agtaaatagc tcaccggtcg taaataattt
     7441 accgatagca attacatctt ggtaatttaa ataccaaact tgtttagttg cacctaccgg
     7501 gtcaataaag tgaatatgcg taccagaaag acctgcaggg tgcggacctt taaagcggtt
     7561 agtttccaca ttattgatct gacggctttg tgccgcattt aaaatatttg agtctgcact
     7621 gccacagaga tgaatcactt ttttactttc aaataaacga cttaatacag ttaagccatc
     7681 aaagaaagct tgttggtgtt ctgcaataat cacttccgga tttgctgcaa gtggattggt
     7741 atccatcgca ttgacaaaaa tagaggaagg ttctgcatct aatgcaggca ctttgctaaa
     7801 cggacgtgta cggaaagccg tccataagcc tgactctaca aggttttgtt ttacttgctc
     7861 tgaagttaat gaagctaatt ggctggcttc ataacgagca aaggtaattt gctcatcacc
     7921 ctcaacttta attactaccg attgaagaac acgcttctcg ccacggttaa ttgccgttac
     7981 ggtaccactt gcaggggcag taaataccac gcccggattc tttttatctt caaaaagaac
     8041 ctgacctttc tttactacat cgccttcacg cactttcatt gaaggacgca tacccacata
     8101 ttcttcacca agcatagcaa cttcagtaac ggtattaccg ttatggatta cttgctcagg
     8161 ttttcccgca atcggaagat ctaagccttt tttgattgta atcatattgt ttgcactact
     8221 tttcttttgg gttaaaaaat atgattgcgc taacgcaatc gcttctattg agccgcagct
     8281 ttacttaaaa ataaacaatg gtcccccacc tatttttaaa agccacatta agttctcaaa
     8341 tctgtgatca acctttatct tttcttaaca aaaggttaac taaaaataat cttcttattt
     8401 tacctaaaaa g
//
LOCUS       DANJWC010000108         8296 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.108, whole genome shotgun sequence.
ACCESSION   DANJWC010000108 DANJWC010000000
VERSION     DANJWC010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8296
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.108"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            118..636
                     /gene="hutZ"
                     /locus_tag="PWX02_001718"
     CDS             118..636
                     /gene="hutZ"
                     /locus_tag="PWX02_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization protein HutZ"
                     /protein_id="HDL1729712.1"
                     /translation="MTTNRQEVLQNRLGPEIEELKQQIKTIMLATIDKDGVPNVSYAP
                     FVINNGEYQVLITTIARHARNLQEVPKVSLMLIEDESKSRQIFARRRLTFDATVRMIE
                     RTDEEWNNSIAALKARHGDLVDELSNMQDFKLFSFKPTAGLFVKGFGKAFDVGVDDLI
                     NIVHLDQGHQTA"
     gene            786..3398
                     /gene="pepN"
                     /locus_tag="PWX02_001719"
     CDS             786..3398
                     /gene="pepN"
                     /locus_tag="PWX02_001719"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HDL1729713.1"
                     /translation="MQPKAKLRKDYRAPDFTITDIYLDFQLDPDRTFVTSKLTVVRKN
                     PEATTLRLDGHSFDFLSLKYNGEPFSAFQKDDESLTLNLADVPAERFELEIETALNPA
                     QNTSLQGLYKSGDGFCTQCEAEGFRQITYMLDRPDVLAKYTTKITASKSKYPYLLSNG
                     NHIAQGDLHDGRHWVEWQDPFFKPSYLFALVAGDFDLLQDKFITKSGREVTLEIYVDR
                     GNLDRAPWAMESLKRSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKF
                     VLAKPETATDTDYLDIEAVIAHEYFHNWTGNRITCRDWFQLSLKEGLTVFRDQEFTSD
                     LWSRSAKRIEDVRLLRTVQFAEDASPMAHPIRPEKVIEMNNFYTVTVYEKGAEIIRMI
                     HTLLGEEKFQQGMQLYVARHDGSAATCEDFVKAMEDASGVNLEQFRRWYSQSGTPELT
                     ISDEYDETRKAYRLHISQMTPATHDQMDKLNLHIPLKVALYGEQDGKRIPLQYELLTV
                     SDVLDVTAEHQTFEFHNVTQRPVPALLCDFSAPVRLDYNYTTEQLLTLLKHAENDFVR
                     WDAAQMLYTNELRENLSRYQQHQPLSFSEGLVNALTFVLDNSASLPELTALTLTLPKE
                     TEFAESFKTIDPQGIAVVREFMQHSIAHALQDKLLAVYHQNQCGEYQVVAEDIAKRAL
                     RNACLSYLAFTDLGNALVHKHYNNADNMTDTLAALQAVTKAQLSCRDALLADFEQKWK
                     HDGLVMDKWFMLQATRPDDDVLSIVQNLMEHPSFNFNNPNRLRSLVGAFCGQNPKAFH
                     AIDGSGYRFLVDTLIKLNESNPQVAARLIEPLIKLSRYDNQRQTLMRRGLERLRELDN
                     LARDLFEKIEKALD"
     gene            complement(3458..3730)
                     /locus_tag="PWX02_001720"
     CDS             complement(3458..3730)
                     /locus_tag="PWX02_001720"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDL1729714.1"
                     /translation="MVAKLFSVYGRVQGVAFRFFTLQEANRLGIKGYVRNRDDGSVEV
                     VAEADEQTIAQLENWLQQGSPAAKVERVIVSDYLGSERFKRFEVRY"
     gene            complement(3784..5418)
                     /locus_tag="PWX02_001721"
     CDS             complement(3784..5418)
                     /locus_tag="PWX02_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005700110.1"
                     /note="is involved in the transport of the murein peptide
                     L-alanyl-gamma-D-glutamyl-meso-diaminopimelate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HDL1729715.1"
                     /translation="MQSKFSLSLLTTSLVLGLSANVFAAKVPEGTTLAEKQEITINNG
                     SEPSSFDPHKIEGVPESQVAYQLFEGLVTKDSDGNIQPGVAESWENTPDYKTWTFKLR
                     QDAKWSNGDAVTAHDFVFAWQRLVNPLTASPYSSYLTYLNVENAQDIIGGKKKPEELA
                     VKAIDDHTFQVQLSQPVPYAVELATHSSLLPVHKATVEKHGDAWVKKGNLVGNGAYTL
                     ADHVINEKIVFERNKNYWNDKETVINKATFLAIANAATDVSRYRAGDLDMTNYALPPE
                     QFATLKKEIPNEVFTARTLSTYYYEINHKRAPFDNVKVREALNLALDRTIITDKVLGQ
                     GQTPTYVFTPPYIHEGEKIQQPAYSKDSMADRKAKALKLLEEAGYSKANPLKFTILYN
                     TNDNHKKIAIAVSSLWKQGTDGIVDVKLENQEWKTFLDTRRNANYDVSRAGWSADYNQ
                     ATTFVNYFLSNSSNNTAFYKSQAYDDAVAESYKATDAAGRAAAYAKAEEQLAKDYAII
                     PIYNYVNPRLVKPYVKGYEGKDPQDDILLRNLYIVK"
     gene            5845..7263
                     /gene="leuC"
                     /locus_tag="PWX02_001722"
     CDS             5845..7263
                     /gene="leuC"
                     /locus_tag="PWX02_001722"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDL1729716.1"
                     /translation="MQQAKTLYQKLFDSHVVYEAEGETPIIYINRHLIHEVTSPQAFD
                     GLRVAGRQVRQVSKTFGTMDHSISTQVRDVQQLEGQAKIQVLELEKNCEETGIPLFDI
                     TKKQQGIVHVMGPEQGLTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTL
                     KQARAKSMKVEVRGKVNPGITAKDIVLAIIGKTTMAGGTGYVVEFCGEAIRDLSMEGR
                     MTVCNMAIEFGAKAGIVAPDETTFAYLKDRPNAPKGQDWEDAIEYWKTLKTDEGAVYD
                     AEVILEAKDIAPQVTWGTNPGQVIGIDDVIPNPEDMADPVTRASAEKALAYIGLEAHT
                     DMKDVPVDQVFIGSCTNARIEDLRAAAAVMKGRKKADNVKRILVVPGSGLVKEQAEKE
                     GLDKIFIEAGAEWRNPGCSMCLGMNDDRLGEWERCASTSNRNFEGRQGRNGRTHLVSP
                     AMAAAAAVFGKFMDIRKVSLNA"
     gene            7276..7878
                     /gene="leuD"
                     /locus_tag="PWX02_001723"
     CDS             7276..7878
                     /gene="leuD"
                     /locus_tag="PWX02_001723"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDL1729717.1"
                     /translation="MAGIKQHSGLVVPLDAANVDTDAIIPKQFLQAITRVGFGKHLFH
                     EWRYLDAEETQINPDFVLNFPQYQGATILLARKNLGCGSSREHAPWALADYGFKVMIA
                     PSFADIFYNNSLNNHMLPIKLTEEEVEEIFQWVWANEGKQIHVDLEAMTVTVGEKVYT
                     FELDEFRRHCLLNGLDNIGLTLQHEAAIAAYEKNIPAFLR"
     gene            complement(7950..>8296)
                     /locus_tag="PWX02_001724"
     CDS             complement(7950..>8296)
                     /locus_tag="PWX02_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729718.1"
                     /translation="ISQAELNNPKDFKRYSYYCKNFATGSSSYLATYFPLWSESRKQE
                     NFGIYFQLDGGKVYPFDHLANISLNARATKFEVRYRSYQPIEGSFVELVAREFTSTYY
                     KNNVPWLECREG"
BASE COUNT         2457 a         1750 c         1694 g         2395 t
ORIGIN      
        1 ttaataatat gtattatcag ttacatcaca tttatagtca ataatgattc tcatatggat
       61 tgaaagtttt cactctttat agtaatctaa aaaacactat ttaaattgag gtattttatg
      121 acaaccaacc gtcaagaggt cttacaaaac cgtttaggtc cagaaattga agaattaaaa
      181 caacaaatca aaactataat gcttgcaacc atagataaag atggtgtacc gaatgtgagc
      241 tatgcccctt ttgtaattaa taacggcgaa tatcaagtct taatcactac tattgcccgt
      301 cacgctcgta atttacaaga ggtgccaaaa gtctctttaa tgctaattga ggatgaaagt
      361 aagagccgtc aaatttttgc ccgtcgtcgc ttaacctttg acgcaacagt gcgaatgatt
      421 gaacgtactg atgaggaatg gaataatagc attgcagcac ttaaagctcg tcacggcgat
      481 ttagttgatg aactgtcaaa tatgcaagat ttcaagctct ttagctttaa gccaacggca
      541 ggcttatttg taaaaggatt tggtaaagcc tttgatgttg gtgttgatga cttaattaat
      601 atcgttcatt tagaccaagg ccaccaaact gcctaattgc gaaataaact cacggctata
      661 aataatccta tttatagccg tttttattgc gacaagcggt caaatttttt ggaaaattta
      721 ccgctatttt agtacaataa cgacactatt ctatgagcta aacgcaacta aaaaaaggaa
      781 atcctatgca acctaaagcg aaactcagaa aagattatcg agctcccgat ttcactatta
      841 ctgatattta tcttgacttc caacttgacc cagatcgcac ttttgtcacc tcaaaactaa
      901 ctgtcgtgcg taaaaatcct gaggcgacaa cacttcgttt agacggtcat agtttcgatt
      961 tcctttcgct taaatataac ggcgagccgt tttccgcctt tcaaaaagat gatgaatctt
     1021 taaccttaaa tttagccgat gtgcctgccg agcggttcga gcttgaaatt gaaaccgcat
     1081 taaatccggc acaaaacacc tcgttacaag gtttgtataa atcgggcgac gggttttgca
     1141 cccagtgtga ggctgaaggt ttccgccaga ttacctatat gctcgaccgc cctgatgtac
     1201 ttgcaaaata tacgacaaaa atcaccgcta gtaagagcaa atacccgtat ctgctttcta
     1261 acggcaacca cattgcccaa ggcgacttgc acgatggcag acactgggtg gaatggcaag
     1321 atccgttctt taaaccaagc tatttatttg ccttggttgc gggggatttt gacttactcc
     1381 aagataaatt catcaccaaa agcggacgtg aagtcacgct tgaaatttat gtcgatcgtg
     1441 gaaatttaga ccgtgcacct tgggcgatgg agagcttaaa acgttcaatg aaatgggatg
     1501 aagagcgttt cggtttggaa tacgatttag atatttatat gattgttgcc gttgatttct
     1561 tcaatatggg agcaatggaa aacaaagggt taaatatttt caattccaaa tttgtattgg
     1621 caaaaccgga aaccgcaacc gatacggatt atttagatat tgaagcggtg attgcccacg
     1681 aatacttcca taattggacg ggcaaccgca ttacctgccg tgattggttc caactaagct
     1741 taaaagaggg cttaaccgtc ttccgtgatc aggaatttac ctccgattta tggtctcgtt
     1801 cggcaaaacg gattgaagat gtgcgtttac tgcgtaccgt acaatttgcc gaagatgcca
     1861 gcccgatggc tcacccgatt cgcccggaaa aagtgattga aatgaataat ttctacaccg
     1921 tgactgtgta tgaaaaaggg gcggaaatta ttcgtatgat tcacaccctg ttaggtgaag
     1981 aaaaattcca gcaaggtatg caactttatg tggcaagaca cgatggttct gcggcaactt
     2041 gtgaagattt cgttaaagca atggaagatg cttcaggcgt taatcttgaa caattccgcc
     2101 gttggtatag ccaatccggc acgcctgagc tcaccatttc cgatgaatat gatgaaaccc
     2161 gcaaggcata ccgcttgcat atttcacaaa tgacaccggc aacccacgat cagatggata
     2221 aactcaatct gcatattccg cttaaagtag cgttatatgg tgaacaggac ggtaaacgca
     2281 ttccgttaca atatgaatta ttgaccgtaa gcgatgtgtt agatgtaacc gcagaacacc
     2341 aaacctttga gtttcataat gtaacacaac gcccggtgcc tgcattactg tgtgatttct
     2401 ctgcaccggt acggttagat tacaactata ccaccgagca gcttttaacc ttactcaaac
     2461 acgctgaaaa tgattttgta cgttgggatg cagctcaaat gctctatacc aatgaactgc
     2521 gtgaaaattt aagccgttat cagcaacatc aaccactcag cttctcagaa gggttggtga
     2581 atgccctaac gttcgtgttg gataactcag caagcctgcc ggaactaact gcattaaccc
     2641 tcacattgcc aaaagaaacc gagtttgcag aatcgttcaa aaccattgac ccacaaggta
     2701 ttgcggttgt gcgtgaattt atgcagcatt ccattgctca cgccttgcaa gataagttat
     2761 tagcggtata tcaccaaaac caatgcgggg aatatcaagt cgtggctgaa gatattgcta
     2821 aacgagcctt gcgtaatgct tgcttaagct atttagcctt tactgattta ggcaacgcgt
     2881 tggtgcataa gcattacaat aatgccgata atatgactga tacgcttgct gcattacaag
     2941 ccgtaaccaa agcccagcta agttgccgtg atgcattatt agccgatttt gagcaaaaat
     3001 ggaaacatga tggtttagtg atggataaat ggtttatgct acaagccacc cgaccggacg
     3061 acgatgtgtt atccatcgtg caaaatttaa tggaacaccc aagttttaac tttaataacc
     3121 cgaaccgttt acgttcatta gtaggtgcat tctgcggtca aaacccgaaa gccttccacg
     3181 ccattgacgg ttcaggctat cgtttcttgg tagatacgct gattaaactt aacgaaagta
     3241 acccacaagt tgctgctcga cttattgagc cgttaattaa actctcacgc tacgataacc
     3301 aacgccaaac cttgatgcgt cgtggtctag aacgcctgcg tgagctggat aatttagcac
     3361 gagatctgtt tgagaaaatt gaaaaagcgt tagattaaac caagcaatac aagcggtgtc
     3421 gtttgcaaat ttttttacaa atgacaccgc ttgttagtta ataccttacc tcaaaccttt
     3481 taaacctttc tgaaccaaga taatcgctca caattacccg ttccaccttt gcagcaggtg
     3541 agccttgttg taaccaattt tccagttgag cgattgtctg ttcatccgct tcagctacca
     3601 cttctaccga gccgtcatca cgatttctga catagccttt tatgcctaat cgattcgctt
     3661 cctgcaaggt aaaaaaccga aaagcgacac cttgaacccg tccgtaaacc gagaataatt
     3721 ttgcgaccac tcttccccca taaaaatagg ctgcttattt ttcaataaac agcccataat
     3781 gtcttatttc acgatgtaga ggttacgcag taaaatatca tcttgcggat ctttaccctc
     3841 atagcctttc acgtaaggtt tcaccaaacg tgggtttaca tagttataaa tcggaataat
     3901 cgcataatct ttggctaact gctcctctgc ttttgcataa gcagctgcac gaccagctgc
     3961 gtctgtcgct ttgtaagatt ctgctaccgc atcatcataa gcttggctct tataaaacgc
     4021 agtattgttg ctggagttcg ataagaaata gttcacaaag gtggttgctt ggttatagtc
     4081 tgccgaccaa cctgcacgag aaacatcata atttgcattg cggcgggtat ctaagaatgt
     4141 tttccactct tggttttcta atttcacatc aacaatgccg tctgtgcctt gtttccataa
     4201 tgatgaaact gcaatcgcaa ttttcttgtg gttgtcgttg gtattataaa gaatagtaaa
     4261 ttttaacggg ttcgctttac tgtaaccggc ttcttccaat aatttcaacg ctttggcttt
     4321 tctatctgcc attgaatctt tggaataagc aggttgctgg attttttcac cctcgtgaat
     4381 ataaggcggt gtaaatacat aagttggtgt ttgaccttgt cctagcactt tatcggtaat
     4441 aatggtgcga tctaaagcta agtttaacgc ctcacgcact ttcacattat caaacggtgc
     4501 tcgtttatgg ttaatttcat agtaataagt agaaagggtt cttgccgtaa acacttcgtt
     4561 tggaatctct tttttcaagg ttgcaaactg ctccggtgga agagcatagt tggtcatatc
     4621 caaatcaccg gcacggtaac gtgaaacatc agttgcggca ttagcaatgg ctaagaaagt
     4681 cgctttatta atcacggttt ctttatcatt ccaataattt ttattacgct caaacacgat
     4741 tttttcatta ataacgtgat cggctaaggt ataagccccg tttccaacca gattgccttt
     4801 tttcacccac gcatcgccgt gtttctcaac ggttgctttg tgtacaggta ataaagaaga
     4861 gtgtgttgcc agctcaactg catacgggac aggttggctt aactgcacct ggaaagtatg
     4921 atcatcaatc gctttaactg ccagttcctc cggttttttc ttgccgccaa taatatcttg
     4981 agcattttct acattgagat aagttaaata gcttgaataa ggtgaagccg taagtggatt
     5041 aaccaaacgc tgccaagcaa atacgaaatc gtgagcagtt acggcatcac cgtttgacca
     5101 tttagcgtct tggcgtaatt tgaacgtcca cgttttgtaa tccggtgtat tttcccaact
     5161 ttctgccaca cccggctgaa tattgccgtc agaatctttc gttaccaatc cttcaaataa
     5221 ctgataagca acttgcgatt ccggcacgcc ctcaatttta tgtggatcaa aacttgatgg
     5281 ctcagaacca ttattaatcg taatttcttg tttttctgca agcgttgttc cttcaggcac
     5341 tttggctgca aacacatttg ccgataagcc taacactaat gaggttgtta ataaggaaag
     5401 cgagaatttt gactgcatat ttgatctcca ttgatgaata ttggcttgca accagccgat
     5461 ttgttctaat taattttttt aagtctaggc attattattt tgattgcaag gaatttttta
     5521 aaaaaatgtg atagatatca cctttcaatg caaaaattgc ataaaaaaat cccgaagaaa
     5581 atagctctat aaactctgat aatggctaga taacttgctg taactttcta attattcaga
     5641 ataatattct gcacattatt aacgaatact atttttaggc ttatttagca gaataaaaac
     5701 ccttatttag cataaaaata ctgttttact ttcatttttt taatcgttat attcactcca
     5761 tatcagcaag cggtcatttt tgcaaaattt tttacaaatt taaccgcttg tattaaaagt
     5821 tacccttttg agttggaaca ttttatgcaa caagccaaaa ccctatatca aaaactattc
     5881 gactcacacg ttgtgtatga agccgaaggc gaaacgccga ttatttatat taaccgccac
     5941 ttaattcacg aagttaccag cccgcaagcg tttgacggct tgcgtgttgc cggtcgccaa
     6001 gttcgccaag tcagtaaaac cttcggcaca atggaccaca gtatttcaac ccaagtgcgt
     6061 gatgtacagc aacttgaagg gcaagcgaaa attcaagtat tagaattaga aaaaaactgc
     6121 gaagaaaccg gtatcccact gtttgacatc actaaaaaac aacaaggtat cgttcacgta
     6181 atggggccgg agcaaggctt aactttaccc ggtatgacca ttgtatgtgg tgactcgcac
     6241 accgccactc acggggcctt tggtgcgtta gccttcggta tcggtacttc cgaagtggaa
     6301 cacgttttag ccacccaaac cttaaaacag gcccgtgcaa aaagtatgaa agtggaagtc
     6361 cgtggcaaag taaacccggg cattaccgca aaagatattg tgcttgcgat catcggtaaa
     6421 acaactatgg ctggcggtac aggctatgta gtggaatttt gtggcgaagc aattcgtgat
     6481 ctttcaatgg aagggcgaat gacagtgtgt aatatggcga ttgagtttgg tgcgaaagcc
     6541 ggtattgttg ccccggatga aaccacgttc gcttacttga aagaccgccc aaatgcacca
     6601 aaaggtcaag attgggaaga tgccattgaa tattggaaaa ccctcaaaac cgatgagggt
     6661 gcagtttacg atgcggaagt gatcttagaa gcaaaagaca ttgctccaca agtgacttgg
     6721 ggaaccaacc cggggcaggt gatcggtatt gatgatgtaa ttccaaatcc ggaagatatg
     6781 gccgatcctg ttactcgtgc ctctgcggaa aaagccttag cttacatcgg cttagaagcc
     6841 catacagata tgaaagatgt accggttgat caagtcttta ttggttcttg tactaatgct
     6901 cgtattgaag atttgcgtgc tgcggcagca gtaatgaaag ggcgtaaaaa agccgacaat
     6961 gtaaaacgta ttctcgttgt gccaggttca ggtttggtaa aagagcaagc agaaaaagag
     7021 ggcttggata aaatcttcat tgaagccggt gcagaatggc gtaacccggg ttgctcaatg
     7081 tgtttgggaa tgaacgatga ccgcttgggc gaatgggaac gttgtgcctc tacgtctaac
     7141 cgtaactttg agggcagaca aggacgtaac ggtcgtaccc acttagtcag cccggcaatg
     7201 gcggctgcgg cggcggtgtt cggtaaattt atggatattc gcaaagtgtc attaaacgca
     7261 taataggagg aaaaaatggc aggtattaaa caacattcag gcttggtcgt tcctcttgat
     7321 gcagcgaacg tagacaccga cgcaatcatt ccaaaacaat ttttgcaagc cattactcga
     7381 gtcggctttg gtaagcacct tttccacgaa tggcgttatt tagatgcgga agagactcaa
     7441 atcaaccccg atttcgtact gaacttccca caatatcaag gtgcaaccat tttactcgcc
     7501 cgtaaaaatt taggctgtgg ctcttctcgt gagcacgccc cttgggcatt ggcagattac
     7561 ggttttaaag taatgatcgc cccaagtttt gcggatattt tctacaataa cagcttaaat
     7621 aaccatatgt tgccgattaa attaaccgaa gaggaagtcg aagagatctt ccaatgggtg
     7681 tgggcaaatg aaggaaaaca gatccacgtt gatttagaag cgatgactgt gactgttggc
     7741 gagaaagttt atacctttga gttagacgaa ttccgccgcc actgcttatt aaacggtttg
     7801 gataatatcg gcttaaccct acaacacgaa gctgcgattg ctgcttatga gaaaaacatt
     7861 ccggcatttt tacgctaatt aaaatacaaa aaaacggcta acatctttaa ggcgttagtc
     7921 gttttctttt tagccatttt ctttggcaat tagccttctc ggcactctaa ccaaggcaca
     7981 ttatttttgt aataagttga agtaaactct cgtgccacta actctacaaa actgccttca
     8041 atcggctgat aagaacgata acgcacttca aattttgttg ctcttgcatt caaactgatg
     8101 ttggcaagat ggtcgaacgg ataaaccttt ccgccatcaa gttgaaaata gataccaaaa
     8161 ttctcttgct tacggctttc agaccaaagt ggaaaatagg ttgccaaata tgaactgctg
     8221 cctgttgcaa aattcttaca ataatagcta taacgcttaa aatctttcgg attattcaat
     8281 tccgcttggc tgatat
//
LOCUS       DANJWC010000109         8285 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.109, whole genome shotgun sequence.
ACCESSION   DANJWC010000109 DANJWC010000000
VERSION     DANJWC010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8285
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.109"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..634
                     /locus_tag="PWX02_001725"
     CDS             <1..634
                     /locus_tag="PWX02_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HDL1729719.1"
                     /translation="LADLIQEGNIGLMKAVKRFDPNVGVRLVSFAVHWVKAEIHEYVL
                     KNWRIVKVATTKAQRKLFFNLRKNKNRLAWFNEEEIKKVADDLGVSVEEVREMESRMT
                     GQDLGFDLPAGDDDEDSYAPSMYIEDSSSNFADDLEDEQHNGQATAQLAYALATLDER
                     SQDIIKTRWLDDNKATLQDLADKYNISAERVRQLENAALKKIKEAISLDG"
     gene            775..1083
                     /locus_tag="PWX02_001726"
     CDS             775..1083
                     /locus_tag="PWX02_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3144 domain-containing protein"
                     /protein_id="HDL1729720.1"
                     /translation="MINITDKQIDPTFYQRADGFINVANAHLKNIAPNQVSNAMLFGC
                     ARFNAYVAASKAEYKQQLADSREEVIQYFVEQYKEMLTANLDEYIQNFERYIEGKKAD
                     "
     gene            complement(1101..1403)
                     /locus_tag="PWX02_001727"
     CDS             complement(1101..1403)
                     /locus_tag="PWX02_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HDL1729721.1"
                     /translation="MNTYNNTLNNKNLEKKQLLMAIQAIPASQDFIWDGKDDDERPVT
                     PSEFSIAMKKRGRPAGSKKELVSLRLDKSVLEAFRASGKGWQSRINQVLSDFVKQV"
     gene            complement(1375..1665)
                     /locus_tag="PWX02_001728"
     CDS             complement(1375..1665)
                     /locus_tag="PWX02_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HDL1729722.1"
                     /translation="MKIYTWDETKRKINLAKHGLDFADADEVLSSPYRFDVEVIRNNE
                     FRIQSFAYSFGRLLVLSLAYTEREDYIRVISFRRASKAEREAYYEYLQQYFE"
     gene            complement(1803..2342)
                     /locus_tag="PWX02_001729"
     CDS             complement(1803..2342)
                     /locus_tag="PWX02_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HDL1729723.1"
                     /translation="MLDLTKIKLVITDVDGVLTDGGMYYTDQGEVMKRFHVHDGLGVK
                     MLQSCGIKVAVLSGGDTALLRKRLEVLKIDLALLGKMEKRSACFELMQQAGVTPEQTA
                     YIGDDTLDLPAFEVCGIAIATRNALDYIKEKADWVLQKAGGEGAFREVSDQILEAQGF
                     GEIFKTADGFFTVAGKMAQ"
     gene            2413..3096
                     /locus_tag="PWX02_001730"
     CDS             2413..3096
                     /locus_tag="PWX02_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDL1729724.1"
                     /translation="MSIAENLSQIHQQIQQISQQHQRDDVRLLAVSKTKPVEAIQEAI
                     EGGQRAFGENYVQEAIEKIECFANRSDLEWHFIGPLQSNKTKLVAAHFDWIQTVDRLK
                     IAERLSEQRPIGKAPLNVLIQINISDEASKSGIEPDEMLSLAQAISQLPNLKLRGLMA
                     IPKPESEPEQQKIAFCKMQQLFHRLQAEFEGIDTLSMGMSDDMQAAIECGSTMVRIGT
                     AIFGARDYR"
     gene            3287..5134
                     /gene="typA"
                     /locus_tag="PWX02_001731"
     CDS             3287..5134
                     /gene="typA"
                     /locus_tag="PWX02_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDL1729725.1"
                     /translation="MQNVDINKLRNIAIIAHVDHGKTTLVDKLLQQSGTFGDTRGDVD
                     ERVMDSNDLEKERGITILAKNTAINWNGYHINIVDTPGHADFGGEVERVLSMVDSVLL
                     VVDAFDGPMPQTRFVTQKAFAHGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLGATD
                     EQLDFPIIYASALNGVAGLEHEELAEDMTPLYEAIVKHVQPPQVELEAPFQMQISQLD
                     YNNYVGVIGIGRIKRGSVKPNQAVTVVGSDGKTRQGRIGQVLGHLGLQRFEATEAFAG
                     DIVAITGLGELNISDTICDINAVEALPALSVDEPTVTMFFCVNTSPFCGQEGKFVTSR
                     QILERLNKELVHNVALRVEETPNPDEFRVSGRGELHLSVLIENMRREGYELAVSRPKV
                     IYREEDGKKQEPFEQVTIDIEEQHQGSVMEALGIRKGEVRDMIPDGKGRTRLEYVIPS
                     RGLIGFRNEFMTMTSGTGLLYSTFSHYDDVKPGEIGQRKNGVLISNATGKALAYALFG
                     LQERGKLMIDHGVDVYEGQIIGIHSRSNDLTVNCLQGKKLTNMRASGKDDAIVLTTPV
                     RFTLEQALEFIDDDELVEVTPQSVRIRKRLLTENDRKRASRTTTSTSTH"
     gene            complement(5183..5620)
                     /gene="rimI"
                     /locus_tag="PWX02_001732"
     CDS             complement(5183..5620)
                     /gene="rimI"
                     /locus_tag="PWX02_001732"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL1729726.1"
                     /translation="MIEQIQAEDFEQLFEIEQQAHLVPWSKGTLLNNQGEKYLNLKVV
                     EQNKIVAFAISHIVLDEATLFNIAVAPDFQGKGIGKRLLSELILQLQQRKLTTLWLEV
                     RESNHSARKLYDSLGFNEVTTRKNYYPTPNGSKENAVVMALYL"
     gene            complement(5613..6035)
                     /locus_tag="PWX02_001733"
     CDS             complement(5613..6035)
                     /locus_tag="PWX02_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HDL1729727.1"
                     /translation="MNRRDLLLQEMGISQWVLTNPQVLKGDAQIRLNNAVKLVVVCEE
                     DHQHSRLFADILRALKLKNSEYQWLTGEQSQRLVFEHSPIFWLIQAEEQAVKMAKSFT
                     NQTAWKNESWQDLAKSTTKRQLWQQIDTFSAQFGEQRD"
     gene            6164..7153
                     /gene="rsmC"
                     /locus_tag="PWX02_001734"
     CDS             6164..7153
                     /gene="rsmC"
                     /locus_tag="PWX02_001734"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HDL1729728.1"
                     /translation="MLSLESEVLERHLALFENKSVLLFGDVRDDFARQLQSRAEKVAV
                     FSSYFDYARHHSEVEFGLECGLSAELAVFYWTKNKQECQFQLLQWLSQCQAGQEMLII
                     GENRAGVRSVEKLLEPFGSIAKIDSARRCGLYHFELQTVPTFDCKKFWKSYRLQDLDV
                     FALPAVFSSAELDNGTKLLLSTFDKADRLKGKVLDLGCGAGVIGATLKQQFPKIKLTM
                     SDIHAMALESGRRTLAENQLEGEVVASDVFSHINERFDLIVSNPPFHDGVDTAYRAVE
                     ELIFQAKNHLTKGGELRIVANAFLPYPDLLDKAFGKHDVLAKSTKFKVYSART"
     misc_feature    7283..7405
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            7427..7900
                     /locus_tag="PWX02_001735"
     CDS             7427..7900
                     /locus_tag="PWX02_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HDL1729729.1"
                     /translation="MKNPIEIYQSQDGKTQVSVQFDKETVWLSLQQMADIFGRDKSVI
                     SRHLRNIYQEGELEREATVAKNATVQIEGAREVVRQVEFYNLDAIISVGYRVNSKAGT
                     QFRIWATARLKDYLVKGYALNERRLQERGIEFEQVIGLLSQYTGCLSLVGGRKFA"
     gene            7967..>8285
                     /locus_tag="PWX02_001736"
     CDS             7967..>8285
                     /locus_tag="PWX02_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1729730.1"
                     /translation="MSINISPLASKNLTEWKLLWQQYLDFYQVNLADDIIQSTWQKVS
                     ESENVKGFGAFVDNRMVGFVHIVIHPNTWNLTECCYLEDLFVLAEYRNQGIGKELIEY
                     IYRY"
BASE COUNT         2490 a         1669 c         1794 g         2332 t
ORIGIN      
        1 gctggcagat ctgatccaag agggtaatat cggcttgatg aaagcggtaa aacggtttga
       61 cccgaatgtc ggcgtgcggt tggtttcttt tgcggttcat tgggtgaaag cggaaattca
      121 cgaatatgtg ctgaaaaact ggcgaattgt gaaagtggca accaccaaag ctcaacgtaa
      181 attattcttt aatttacgca aaaataaaaa ccgcttggcg tggtttaatg aagaagagat
      241 caaaaaagtt gccgatgatt taggggtttc agtggaagaa gtacgagaaa tggaatctcg
      301 aatgaccgga caagatttag gttttgatct gcctgccggt gatgacgatg aagacagcta
      361 cgctccttct atgtatattg aagatagcag ctccaatttc gccgatgact tggaagacga
      421 acaacataat gggcaggcaa ccgctcaact tgcctatgcg ttagccaccc ttgatgaacg
      481 tagccaagac atcatcaaaa cccgctggtt agatgacaat aaagccacac tgcaagattt
      541 agccgataaa tataacatct cggcagagcg ggtaagacag cttgaaaatg ccgcattgaa
      601 gaaaatcaag gaagcgattt cattagacgg ctagaaatgc ctacaagcgg tgattttttt
      661 actcgctacg ctcgcacttc gtgccgtcgg cagagccaac gttcaaacgc aagcgtttgt
      721 gcaaaatttt acgcaaaaag caccgcttgt attttgacta aaaatgagga tactatgatt
      781 aacattaccg ataaacaaat tgacccaact ttctaccagc gagcagacgg ctttattaac
      841 gttgccaacg ctcatttaaa aaacattgcc ccaaaccaag tcagcaatgc gatgttgttt
      901 ggttgtgcca gatttaacgc ctatgttgcg gcaagcaagg cggaatataa acagcagttg
      961 gccgattcca gagaagaagt tattcaatat tttgtggagc aatacaaaga aatgctcact
     1021 gcaaatttag atgaatatat ccaaaacttc gagcgatata ttgaagggaa aaaggcggat
     1081 taaccgcctt tgttttgtgg tcaaacttgt tttacaaaat cacttaaaac ttgattaatt
     1141 ctactttgcc aacctttacc tgaagctcta aaggcttcaa gaacgctttt atcaagacgt
     1201 aacgaaacca attctttctt tgaaccagct ggtctgccac gttttttcat tgcaattgaa
     1261 aactctgatg gcgtaaccgg gcgttcatcg tcatcttttc cgtcccaaat aaaatcttgg
     1321 cttgccggaa tagcttgaat agccataagt agctgttttt tctctaaatt tttattattc
     1381 aaagtattgt tgtaagtatt cataatatgc ctctctttct gctttagatg ctcgccgaaa
     1441 gctaattact ctgatgtaat cttccctttc tgtatatgca agtgataata cgagtaaacg
     1501 tccaaatgaa tatgcaaaag attgtattct aaattcattg ttacgaataa cttcaacatc
     1561 aaatcgatag ggcgatgaga gaacttcatc tgcatcggca aaatctaatc cgtgcttggc
     1621 taaattgatt ttgcgtttag tttcgtccca agtataaatt ttcataatta ttgtatatac
     1681 ataaaataaa tctaacaaga actaaattta atttagcaaa cagtaatttg aaaatcagat
     1741 tagaaataca ttttttcgat gcagatcgca aaaataaaat gaaatttaac tgcttgaaga
     1801 gtctactgag ccattttccc cgctaccgta aaaaaaccat ctgcggtttt aaaaatttca
     1861 ccgaatcctt gagcctccaa aatttggtcg gagacttcac gaaacgcccc ttcaccaccg
     1921 gctttttgca atacccaatc tgctttttct ttgatgtaat cgagggcgtt acgggttgca
     1981 atcgcaatgc cacacacttc aaaagccggt aaatctaagg tgtcatcacc aatgtaagcc
     2041 gtttgctcag gggtaacacc ggcttgctgc attagctcaa agcaggcgga gcgtttttcc
     2101 attttgccta acaaagcgag atcaattttc agcacctcta accgtttgcg gagcaaagcc
     2161 gtatcgccgc ccgagagtac tgccacttta atgccacaac tttgtaacat cttaacgcct
     2221 aaaccatcgt gaacgtggaa acgtttcatt acttcgcctt ggtcggtgta gtacattccg
     2281 ccatcggtta gcacgccgtc cacatcggta atcactaatt taatcttggt gagatctaac
     2341 atttttttgc ctcaaatatg gtatgatttt gccattatag cttttttacc tccgtttacc
     2401 aaaggaaccg aaatgtcgat tgccgagaat ttatcgcaaa tccatcaaca aattcagcaa
     2461 atttcccaac aacaccaacg tgatgacgta cggctacttg ccgtctcgaa aaccaagccg
     2521 gtagaagcca ttcaagaggc gatagagggc ggtcagcgtg catttggtga gaattatgtg
     2581 caggaagcga tcgaaaaaat cgaatgtttt gcaaatcgtt cagacttgga atggcacttt
     2641 attggtccgt tacaatcgaa taaaaccaag ttagttgcag ctcattttga ttggattcaa
     2701 acggtggata ggctcaaaat tgccgagcgt ttaagcgaac aacgcccgat aggaaaagca
     2761 ccgcttaatg tattgattca aattaatatc agcgatgaag cctcaaaatc aggcattgag
     2821 ccggacgaaa tgttatcttt agcccaagcg atttctcaat taccgaattt gaaattacgt
     2881 ggtttaatgg caatcccaaa accggaaagt gagccggagc aacaaaaaat cgctttttgc
     2941 aaaatgcagc aactttttca ccgcttgcaa gcagaatttg agggcattga caccctttca
     3001 atgggaatgt ctgacgatat gcaagccgcc attgaatgtg gttctacaat ggtgagaata
     3061 ggcacggcga tttttggggc gagggattat cgctaaaatt tatcggcata acaaaggaat
     3121 gaatctggca agtagtctta aattgtaaaa tttttgcaaa tttagaccgc ttgttttgta
     3181 gccaaattct gaaaaactct gtataattcg acctccaatt tctagactct cgccggtttt
     3241 gatgggcgag agtttttatt ttcactcaat aaaaaaagat attccaatgc aaaatgtaga
     3301 tattaataaa ttgcgtaaca ttgcaattat tgctcacgtt gaccacggta aaacaacgct
     3361 ggttgataaa cttttacaac aatccggcac ctttggtgac acccgaggcg acgtggacga
     3421 gcgtgtgatg gactccaacg atttggaaaa agagcgtggt attaccattc ttgcaaaaaa
     3481 tacggcaatt aactggaatg gttatcacat caatatcgta gataccccag ggcacgctga
     3541 cttcggtggt gaggttgagc gtgtactttc aatggtagat tcggtgcttt tagtggtaga
     3601 tgcctttgac ggcccgatgc cacaaacccg tttcgttacc caaaaagcct ttgctcacgg
     3661 cttaaagcca atcgtagtta ttaacaaagt ggaccgccct ggcgctcgtc ctgactgggt
     3721 ggttgatcaa gtgttcgatc tcttcgtaaa tttaggtgca acagacgagc aattagattt
     3781 ccctatcatt tacgcttctg cgttaaacgg cgtggctggt ttagaacacg aagagttggc
     3841 agaagatatg accccgcttt atgaagcgat tgtgaagcac gttcagccgc cacaagtgga
     3901 attagaggct ccgttccaaa tgcaaatctc gcaattagac tataacaact atgtaggcgt
     3961 tatcggtatc ggtcgtatca aacgtggttc ggtaaaacct aaccaagcgg taacggtagt
     4021 aggcagcgat ggcaaaaccc gccaaggtcg tatcggtcaa gtattagggc atttaggttt
     4081 acaacgcttt gaagcaactg aagccttcgc aggcgatatc gtggcaatta caggtttagg
     4141 tgaattaaat atttcagata cgatttgtga tattaatgcg gtggaagcgt tacctgcatt
     4201 aagcgtagat gagccaacgg taaccatgtt cttctgtgta aacacctcgc cattctgtgg
     4261 tcaagaaggt aaatttgtaa cttctcgcca aattcttgag cgtttaaaca aagaattagt
     4321 ccacaacgta gcacttcgtg tggaagaaac acccaaccca gacgaattcc gtgtgtcagg
     4381 tcgtggcgaa ttacacttat cggtattaat cgaaaatatg cgtcgtgaag gttacgagtt
     4441 agcagtgtct cgcccgaaag taatctaccg tgaagaagac ggcaaaaaac aagagccgtt
     4501 tgagcaagtg acgattgata tcgaagagca acaccaaggt tcagtgatgg aagcacttgg
     4561 tatccgtaaa ggtgaagtgc gtgatatgat tccggacggc aaaggacgca ctcgcttaga
     4621 atatgtgatt ccaagccgtg gcttaatcgg cttccgtaac gaatttatga cgatgacctc
     4681 aggtacaggt ttactttact caacattcag tcattatgat gacgtaaaac cgggcgaaat
     4741 cggccagcgt aaaaacggtg tgttaatttc taacgcaacg ggtaaggcat tagcatacgc
     4801 attatttggc ttacaagagc gtggaaaatt aatgatcgac cacggtgttg atgtatatga
     4861 agggcaaatt atcgggattc acagccgttc aaacgattta accgtaaact gcttacaggg
     4921 taagaaatta accaatatgc gtgcatcagg taaagacgat gccatcgttt taaccacacc
     4981 ggtgcgtttc actctagaac aagcgttaga gtttattgac gatgacgaat tagtggaagt
     5041 aacaccacaa tcagtgcgta ttcgtaaacg tttattaact gaaaacgacc ggaaacgtgc
     5101 aagccgtact acaaccagta ccagcacgca ctaactgata aaaaagagcg actgaggtcg
     5161 ctcttttggt tttaaattct ccctacaaat ataacgccat taccaccgca ttttctttgc
     5221 taccgtttgg cgtggggtaa tagtttttgc gggtggtaac ttcattaaaa ccgagtgaat
     5281 cgtagagttt tctggcagaa tggttggatt ctcgcacttc caaccacagg gtggtgagtt
     5341 tccgttgttg cagttgcaaa attaattcgg aaagtaaccg cttgccaatg cctttgcctt
     5401 gaaaatcagg agcaactgca atgttaaata aggttgcttc atctagcacg atatgactaa
     5461 tggcaaaagc cacaattttg ttttgctcta ccacttttaa attcaggtat ttttcaccct
     5521 gattatttaa taacgtcccc tttgaccacg gcactaaatg ggcttgttgc tcaatttcaa
     5581 ataactgctc gaaatcttcc gcttgaattt gctcaatcac gctgttctcc aaattgtgcc
     5641 gaaaaagtat caatttgttg ccaaagttgg cgtttggtcg tagatttggc taaatcttgc
     5701 caagactcgt ttttccaagc ggtctgatta gtaaaacttt ttgccatttt gaccgcttgt
     5761 tcttccgctt gaatcagcca aaagatcggc gaatgctcaa acaccaaacg ttgtgattgt
     5821 tcgccagtca gccattgata ttcgctgttt tttaacttta aagcacgcag aatatcggca
     5881 aagagccggc tgtgttggtg atcttcctca cacactacca ccaattttac cgcattgttt
     5941 aggcggattt gagcatcgcc ttttagcact tggggattgg ttagcaccca ttgcgaaata
     6001 cccatttcct gtaacagtaa atcacgacga ttcatttaaa acctcatcaa attttgcaaa
     6061 tagtatcaaa actccgcttg aacagaaact tttttcaaaa aattcaagat ttctgtactt
     6121 aaattcggta tattcacgct aattttttag ataaggtaag aaaatgctct ctttagaaag
     6181 cgaagtactt gaacgccatt tggcgttatt tgaaaacaag tcggtgttat tatttggcga
     6241 tgtgcgagat gattttgccc gccagctgca atctcgtgcg gaaaaggttg cggtattcag
     6301 tagctatttt gattacgcac gccaccatag tgaggttgaa tttggcttag aatgtggttt
     6361 gagtgccgag ttagcggtgt tttattggac gaagaacaag caggaatgcc aattccaact
     6421 gctacaatgg ctgtcgcaat gccaagcagg gcaagaaatg ttgattatcg gcgaaaaccg
     6481 agctggtgtt cgttcggtag aaaaattgct cgaaccgttc ggcagtattg ccaaaatcga
     6541 ctctgcacgc cgttgtggtt tataccattt tgagctacag accgtgccga cattcgattg
     6601 caaaaaattc tggaaatcct accgcttgca agacttagat gtgttcgctc taccagcggt
     6661 ttttagctcg gcggagctgg ataacggcac caaattattg ctttcaacct tcgacaaagc
     6721 agacaggcta aaaggcaaag tgttagattt aggctgtggg gctggcgtga ttggggcaac
     6781 gctcaaacaa caatttccga aaatcaaact gacgatgagc gatattcacg caatggcgtt
     6841 ggaatccggc agacgtactt tggcggaaaa tcagttagag ggggaagtgg tagcaagtga
     6901 tgtgttctct cacattaatg aacggtttga tttaattgtc tccaacccac cattccacga
     6961 cggggtcgat accgcctacc gagcggtgga ggagttgatt ttccaagcta aaaatcacct
     7021 aaccaaaggc ggtgagctgc gaattgtcgc aaacgcattc ttgccttacc cagatttgct
     7081 cgataaagcc ttcggcaaac acgacgtact ggcaaaatcc accaagttta aggtgtattc
     7141 cgcaagaaca taataggcag aacaaacggt tataaaaaag ccgatatttg cttaaaaaag
     7201 tgcttgatat ttctgccggc ttgacgtaaa ttctgttcat actgttttgg agtaatgcaa
     7261 tgaatttaaa ctacacttta catcaccacc acactttaac ctgatatctt tcgggcattt
     7321 ggtgtgtgcg gaagataact cttccgagca agtgtagcaa atcaataaag atctctcgga
     7381 aggcaacttt cgagagattt ttttatagat aaaatttggg aataatatga aaaacccgat
     7441 tgaaatttac caaagccaag acggcaaaac ccaagtttcc gtgcagtttg ataaggaaac
     7501 ggtgtggcta agtttacagc aaatggcaga tatttttgga cgagacaagt ctgttatttc
     7561 tcggcactta cgaaatatct accaagaagg tgaactggag cgtgaggcaa ctgttgcaaa
     7621 aaatgcaaca gttcaaattg aaggtgctag agaagtcgtt cgccaagtgg aattttataa
     7681 tctagatgcg attatttctg ttggctatcg ggtgaattcc aaagctggca ctcagtttcg
     7741 tatctgggca accgctcgcc taaaagacta tctggtaaaa ggctatgccc ttaacgaacg
     7801 ccgtttgcag gaacgaggta tcgagtttga gcaggtgatt ggtttgctca gtcaatacac
     7861 tggttgcctt agccttgttg gtggcagaaa gtttgcctga acaaaaagaa ctgatgatta
     7921 aactgattga acactttatt ttattaaaag cataacagga aaaaatatga gtattaatat
     7981 ttcgccgtta gcctccaaaa atctcactga atggaaacta ttgtggcaac aatatttaga
     8041 tttttatcaa gtgaatttag cagatgatat tattcaatca acttggcaga aagtttcaga
     8101 gagtgaaaat gtaaagggat ttggggcttt tgttgataac agaatggttg gttttgttca
     8161 tattgtgatt cacccaaata cttggaattt aactgaatgc tgttatttgg aagatttatt
     8221 tgttttagct gaatatcgca accaaggcat aggaaaagaa ttaattgaat atatttatcg
     8281 atatg
//
LOCUS       DANJWC010000110         8249 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.110, whole genome shotgun sequence.
ACCESSION   DANJWC010000110 DANJWC010000000
VERSION     DANJWC010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8249
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.110"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            105..1097
                     /locus_tag="PWX02_001737"
     CDS             105..1097
                     /locus_tag="PWX02_001737"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDL1729731.1"
                     /translation="MKKSFILQQQEISFVKNTFTQYLIDKLGIIEVQGPILSEVGNGM
                     QDNLSGIEKAVKVNVKCIPSSTYEVVHSLAKWKRHTLARFGFKEGEGLFVHMKALRPD
                     EDSLDRTHSVYVDQWDWEKVIPEGRRNFDFLKETVNEIYKAIRLTELAVEARFDIPSV
                     LPKQITFVHSEDLVQRYPNMTSKERENAICKECGAVFLIGIGGKLSDGKPHDGRAPDY
                     DDWTTESEQGYKGLNGDILVWNEELGTAFELSSMGIRVDETALRLQVSLTGDEDRLEM
                     DWHKDLLAGRLPLSIGGGIGQSRLVMFLLRKKHIGEVQSSVWPKAVFEEFENIL"
     gene            complement(1221..1640)
                     /gene="atpC"
                     /locus_tag="PWX02_001738"
     CDS             complement(1221..1640)
                     /gene="atpC"
                     /locus_tag="PWX02_001738"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDL1729732.1"
                     /translation="MASQFELTVVSAERKIFEGTVTSVRVSGTDGELGVYAGHAPLLT
                     AIKPGMVKFTLADGKEEFIYVSGGFLEVQPTVVTVLADTAIRGEELDEQRILAAKRKV
                     EETLSKTSDADITAKLAREIAKLRVYELTKTKLANKR"
     gene            complement(1668..3041)
                     /gene="atpD"
                     /locus_tag="PWX02_001739"
     CDS             complement(1668..3041)
                     /gene="atpD"
                     /locus_tag="PWX02_001739"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011978979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDL1729733.1"
                     /translation="MATGKIVQIIGAVIDVEFPQDAVPKVYDALKVESGLTLEVQQQL
                     GGGVVRCIALGSSDGLKRGLKVENTNKAIEVPVGTKTLGRIMNVLGEPIDEAGPIGEE
                     ERWTIHRAAPSYEEQANSTELLETGIKVIDLVAPFAKGGKVGLFGGAGVGKTVNMMEL
                     IRNIAIEHSGYSVFAGVGERTREGNDFYHEMKDSNVLDKVSLVYGQMNEPPGNRLRVA
                     LTGLTMAEKFRDEGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEMGVL
                     QERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDSTVVLSRNIASLGIYPAVDP
                     LDSTSRQLDPLVVGEEHYNVARGVQGTLQRYKELKDIIAILGMDELSEDDKRLVARAR
                     KIERFLSQPFHVAEVFNGVPGKFVPLKETIRGFKGILEGEYDHIPEQAFYMAGSIDEV
                     VERAKQM"
     gene            complement(3062..3928)
                     /gene="atpG"
                     /locus_tag="PWX02_001740"
     CDS             complement(3062..3928)
                     /gene="atpG"
                     /locus_tag="PWX02_001740"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDL1729734.1"
                     /translation="MAGAKEIRTKIASVRNTQKITKAMEMVAASKMRKTQERMAASRP
                     YAESIRKVISHIAKGNIEYKHPFLTPRPVKKVGYLVVSTDRGLCGGLNINLFKTVLHE
                     LKEKDDQGVKSRLAVVGNKGISFFNPMGLEIKGHINGLGDTPAMEDLVGIVNGMVNAY
                     REGEIDEVYVVYNRFINTMSQKPTVQQLLPLPALENDSLEQTGSWDYLYEPNPQALLD
                     SLLVRYLESQVYQAVVDNLASEQAARMVAMKAATDNAGNLINELQLVYNKARQASITN
                     ELNEIVAGAAAI"
     gene            complement(3954..5495)
                     /gene="atpA"
                     /locus_tag="PWX02_001741"
     CDS             complement(3954..5495)
                     /gene="atpA"
                     /locus_tag="PWX02_001741"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDL1729735.1"
                     /translation="MQLNSTEISELIKKRIAQFNVVSEAQSTGTIVSVSDGVIRIHGL
                     SDVMQGEMIALPGNRYAIALNLERDSVGAVVMGPYADLAEGMQVQCTGRILEVPVGRG
                     LLGRVVNTLGQPIDGKGEIQNDGFSPIEVIAPGVIDRQSVDQPVQTGYKAVDSMVPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTIANVVRKLEEHGAL
                     ANTIVVVASASESAALQYLAPYAGCAMGEYFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERASRVNADYVERFTNGAVKGQTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLESGLFNAGIRPAVNPGISVSRVGGSAQTKLVKKLAGG
                     IRTALAQYRELAAFAQFASDLDEATRKQLSHGQKVTELLKQKQFEPMSVAQLGLSLAA
                     AEYGYLDDIPVERVGSFEANLLAYAQSNYGEFMQELSKSGNFNDEIKDKLNEILSGFK
                     KNSAW"
     gene            complement(5508..6041)
                     /gene="atpH"
                     /locus_tag="PWX02_001742"
     CDS             complement(5508..6041)
                     /gene="atpH"
                     /locus_tag="PWX02_001742"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDL1729736.1"
                     /translation="MSELSTVARPYAKAAFDFALEQGQLEQWQQMLTFSAAVANDEQV
                     ASFLNSSLASGQISETFIKICGEQLDQYGQNFIRVMADNKRLAVLPAVLNAFLELRAE
                     QESIKDVEVVSADKLTDAQEAKIANAMEKRLGTKVRITSSIDSSLIAGVIIRYDDVVI
                     DGSSRGQLNRLSQELSL"
     gene            complement(6055..6525)
                     /gene="atpF"
                     /locus_tag="PWX02_001743"
     CDS             complement(6055..6525)
                     /gene="atpF"
                     /locus_tag="PWX02_001743"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDL1729737.1"
                     /translation="MNLNATLIGQLIAFALFVAFCMKFVWPPLIRAIEERQANIASAL
                     ASAEKAKQEQADSKAAADQEILRAKEEAQNIIDLATKRRNEILESVQAEAEVERQRII
                     EQGYAEVESERKRVQEELRQKVAALAIAGAEKIVGRSVDTAANNDIIDKLVAEL"
     gene            complement(6586..6840)
                     /gene="atpE"
                     /locus_tag="PWX02_001744"
     CDS             complement(6586..6840)
                     /gene="atpE"
                     /locus_tag="PWX02_001744"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDL1729738.1"
                     /translation="MESVITATIIGASILLAFAALGTAIGFAILGGKFLESSARQPEL
                     ASSLQTKMFIVAGLLDAIAMIAVGISLLFIFANPFIDLLK"
     gene            complement(6958..7746)
                     /gene="atpB"
                     /locus_tag="PWX02_001745"
     CDS             complement(6958..7746)
                     /gene="atpB"
                     /locus_tag="PWX02_001745"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDL1729739.1"
                     /translation="MAGTTAEYISHHLSFLSTGEGFWAVHLDTLFFSLVAGAIFLWVF
                     SRVAKNATTGVPGKLQCFVEIVIEWVNGLVKDNFHGPRNVIAPLALTIFCWVFIMNAI
                     DLIPVDFLPQLANLFGIHYLRAVPTADISATLGMSICVFALILFYTVKSKGFGGLVKE
                     YTLHPFNHWAFIPVNFILETVTLLAKPISLAFRLFGNMYAGELIFILIAVMYMADNFA
                     LQALGIPLHLVWAIFHILVITLQAFIFMMLTIVYLSIAYNKADH"
     gene            complement(7769..8152)
                     /locus_tag="PWX02_001746"
     CDS             complement(7769..8152)
                     /locus_tag="PWX02_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HDL1729740.1"
                     /translation="MSAVINRAKSQYKQALKVEFFLLCVFALGVSLWKMNFNFSFLGG
                     ALASFLPHLVFVYWVFFKKSAKNQSKMTAFYRGEGMKWLVTIILVIGCFKLIPALDVV
                     LFFVGYFIALFLNNMIPFVLSKHHH"
BASE COUNT         2453 a         1857 c         1537 g         2402 t
ORIGIN      
        1 tattgcattt tttagatatt tctttaaatt agcactcatc aattcttaaa tgcaatcaag
       61 cacgagaaat aaaaatcaac attctttaat taggaaagca aacaatgaaa aaatctttta
      121 ttttacagca acaagaaatc agtttcgtta aaaacacctt tacgcaatac cttattgata
      181 aattaggcat tatcgaagta caaggtccta ttttgagcga agtcggcaac ggtatgcaag
      241 acaacctttc cggtatcgaa aaagcggtaa aagtaaacgt aaaatgtatt ccaagctcta
      301 cttatgaagt ggttcattct cttgcaaaat ggaaacgcca caccttagct cgctttggat
      361 ttaaagaagg agaagggtta tttgttcata tgaaagctct tcgccctgat gaagattctt
      421 tagatcgcac acactcggta tatgttgacc aatgggactg ggaaaaagtc attcctgaag
      481 gtcgccgcaa cttcgatttc ttaaaagaaa ccgtaaacga aatctataaa gcaattcgtt
      541 taaccgaatt agcggtggag gctcgtttcg atattccatc tgtattgcct aaacaaatta
      601 ctttcgttca cagcgaagac ttagtgcaac gctatccgaa tatgaccagc aaagagcgtg
      661 aaaatgctat ctgtaaagag tgcggagcgg tgttcttaat cggtatcggc ggaaaattat
      721 cagacggtaa acctcacgat ggacgtgctc cggactacga tgactggaca accgaatccg
      781 aacaaggcta caaaggttta aacggtgata ttctcgtttg gaacgaagaa ctcggcacag
      841 cgtttgagct ttcctcaatg ggtattcgtg tggatgaaac tgccttacgc ctacaagtct
      901 ctttaaccgg cgacgaagac cgcctcgaaa tggattggca caaagattta ttagccggca
      961 gactcccgct ttctatcggc ggcggtatcg gtcaatctcg cttagtgatg ttcttactac
     1021 gcaaaaaaca catcggtgag gtgcaatcca gtgtctggcc aaaagcagta tttgaagagt
     1081 ttgagaatat tctttaataa aacaaaaaag cgaaccttag ggttcgcttt tgctttctac
     1141 aagcggtctt attttgcaaa aaatttactg tttttaaccg cttgtaaaac aaaaccccac
     1201 tttcgagggg ttttttgata ttaacgttta ttcgctaatt tggttttagt gagttcgtaa
     1261 acacgcagtt tcgcaatttc tctcgctaac ttcgcggtta tatccgcatc gctggttttt
     1321 gaaagtgttt cttctacttt gcgttttgcg gcaagaatac gctgttcgtc tagctcttcg
     1381 ccacgaattg cggtatctgc caacactgtt actacagtcg gttgtacttc aaggaaacca
     1441 cctgaaacgt aaataaactc ttctttgccg tcagctaaag tgaatttaac cattcccggt
     1501 ttaattgcgg ttaatagcgg tgcatgccct gcatacaccc ctaattcacc gtctgttcct
     1561 gaaacacgca cgctggtaac tgtgccttca aagattttac gttctgcact cactaccgtg
     1621 agttcaaatt gagatgccat gttgttctcc tttagtttag aacaaaatta catctgtttt
     1681 gctctttcaa ccacttcgtc aattgaaccc gccatataga acgcttgttc cggaatatgg
     1741 tcgtattcgc cttctaaaat gcctttaaag ccacgaattg tttcttttaa cggtacgaat
     1801 ttgcccggta cgccgttgaa tacttccgca acgtggaatg gttgtgaaag gaaacgttcg
     1861 attttacgcg cacgagcaac aaggcgtttg tcgtcttccg ataattcatc catacccaaa
     1921 attgcaatga tgtcttttaa ctctttataa cgttgtaatg taccttgtac accacgagca
     1981 acgttatagt gttcctcacc aaccacgagt ggatctaact gacgagaggt tgagtcaaga
     2041 gggtcaaccg ccgggtaaat accgagtgaa gcgatattac ggcttaatac caccgttgag
     2101 tctaagtggg caaaggtagt tgccggagac gggtcagtta agtcatccgc cggaacatat
     2161 accgcttgca cagaggtaat agagccggtt ttggttgagg taatacgctc ctgtaatacg
     2221 cccatctctt ctgcaagagt cggctgataa cctaccgcag acggcatacg acctaataac
     2281 gcagatactt cggtaccagc taaagtataa cggtagatgt tatccacgaa gaataatacg
     2341 tcacgacctt catcacggaa tttttctgcc atggttaagc cggttaatgc cacacgcaga
     2401 cggttgcccg gtggttcgtt catttgaccg taaaccagcg atactttgtc taatacgtta
     2461 gagtctttca tttcgtggta gaagtcatta ccctcacgag tacgctcacc tacaccggcg
     2521 aataccgagt aaccgctatg ttcgatagcg atgttacgga ttaactccat catatttacg
     2581 gttttaccta cacccgcacc accgaataag ccaactttac cacccttagc aaatggagca
     2641 actaagtcga taactttgat accggtctct aaaagctcgg tgctgttcgc ttgttcttcg
     2701 tagcttggtg ctgcacggtg gattgtccaa cgttcttctt caccgattgg gcctgcttca
     2761 tcaattggct cacctaatac gttcataata cgaccgagtg tttttgtacc aaccggtact
     2821 tcaatcgctt tattcgtatt ttcaactttt aagccacgtt ttaaaccatc agatgaacca
     2881 agtgcgatac aacggaccac accgccacct aattgttgtt gaacttcaag ggttaatcct
     2941 gattcaactt ttaatgcatc ataaactttt ggtactgcat cttgcggaaa ctcaacatcg
     3001 attaccgcac cgatgatctg tacaattttt cccgttgcca ttaccgttcc tctattcatt
     3061 gttaaattgc tgcggcaccc gcgacaattt catttaattc attcgtaata cttgcttgac
     3121 gagctttgtt atacactaac tgtaactcat taatcagatt acctgcgtta tcggttgctg
     3181 ctttcattgc caccattcga gcagcctgtt cagacgcaag attatctacc actgcctgat
     3241 aaacttgaga ttctaaataa cgaaccagta agctgtctaa taacgcttgt ggatttggtt
     3301 catagagata atcccaagaa ccagtttgct ctaatgagtc attttccagt gcaggcaaag
     3361 gaagcaactg ttgtactgtc ggtttttgtg acatcgtgtt tataaaacgg ttatatacca
     3421 catacacttc atcaatttcg ccttcacggt aggcatttac cataccatta acaataccga
     3481 ctaaatcttc cattgccggt gtatcaccca atccattgat atgaccttta atctctagcc
     3541 ccattgggtt aaaaaaggag atccctttat ttcccaccac agcaagtcga gacttaacac
     3601 cttggtcatc tttttctttc aattcatgta aaacggtttt aaataaattg atatttaagc
     3661 caccacataa accgcgatcg gttgaaacta ctaaatagcc aacttttttt accggacgtg
     3721 gggtcaaaaa tgggtgttta tactcaatgt tacctttggc aatatggcta attaccttgc
     3781 gaatactttc agcataaggg cgagaagccg ccatacgctc ttgggtttta cgcatttttg
     3841 atgcggcaac catttccatc gctttggtaa ttttttgtgt attacgaaca cttgcaattt
     3901 tggttcttat ctctttagca cctgccatat tatttctccg ctaaaaagtt agattaccaa
     3961 gcactgttct ttttgaaacc agataaaatc tcgttcaatt tatctttaat ttcatcgttg
     4021 aaattgccgg attttgacag ttcttgcata aactcgccgt agttactttg agcataagct
     4081 aataagtttg cttcaaatga gccaacacgc tcaaccggaa tatcatctaa gtaaccgtat
     4141 tcagctgctg ctaatgataa acctaattgt gcaacagaca tcggctcgaa ttgtttttgt
     4201 ttcaacaatt cagttacttt ttgaccgtgt gaaagctgtt tacgtgttgc ttcatcaaga
     4261 tctgatgcga actgggcaaa tgccgctaat tcacggtatt gagcaagtgc ggtacggata
     4321 ccaccggcaa gcttcttaac cagtttagtt tgtgcagaac cacctacacg ggaaaccgag
     4381 atacccgggt ttaccgcagg gcgaataccg gcattgaata agcctgattc taagaaaatc
     4441 tgaccatcgg taattgaaat tacgttagtc ggtacgaagg ctgaaacgtc acctgcttgg
     4501 gtttcgatga tcggtaatgc ggttaaagag ccggtttgac ctttcacagc accgttagtg
     4561 aaacgttcta catagtctgc atttacacga gacgcacgct ctaataagcg agagtgtaga
     4621 tagaacacat cacccgggaa tgcttcacga cccggtggac ggcgtaataa caatgaaatt
     4681 tgacggtaag caactgcttg cttagacaaa tcatcgtaaa cgatcaacgc atcttcacca
     4741 cggtcacgga agtattcacc cattgcacaa cctgcataag gtgcaagata ttgtaatgca
     4801 gcagattcag acgcagaagc cactacaacg atagtgttcg ctaatgcacc gtgttcttct
     4861 aatttacgca ccacgttggc gatagttgat gccttttgac cgattgcaac gtagatacat
     4921 ttaatgcctg aatcacgttg gttgatgatc gcatcaatcg ctaatgcggt tttaccggtt
     4981 tgacggtcac cgataatcaa ttcacgctga ccacgaccga ttggtaccat tgagtccacc
     5041 gctttgtaac cggtttgaac cggttgatca accgattgac ggtcgataac gcccggtgcg
     5101 atcacttcga ttggtgagaa accgtcattt tggatttcgc ctttaccatc gattggctga
     5161 cctaaggtat ttacaacacg acctaataag ccacgaccaa ccggcacttc taaaatacga
     5221 ccggtacatt gtacttgcat accttctgct aaatcggcat aaggtcccat tacaactgca
     5281 ccaacagaat ctctttctaa gtttaatgca atcgcataac gattgcccgg taatgcaatc
     5341 atttcacctt gcattacatc agataagccg tggatacgaa taaccccgtc acttaccgaa
     5401 acgattgtcc ctgtgctttg agcttcactc accacattaa actgggcaat acgtttttta
     5461 atcaattcac taatttcagt tgaatttagt tgcatttttt attcctctta caagctcaac
     5521 tcttgactca agcggtttag ctgtccacga ctactaccat caatcactac atcatcataa
     5581 cgaatgatca cacccgcaat taatgaacta tctattgatg aagtaatacg tactttcgtt
     5641 ccgagtcgct tttccatagc attggcaatt tttgcttctt gtgcatctgt taatttatct
     5701 gcagaaacaa cctcaacatc tttaattgat tcctgttctg ctcttaattc taaaaatgca
     5761 ttcaataccg caggtaatac tgctaaacgc ttgttatcag ccattacgcg aatgaaattt
     5821 tgcccatatt gatctaattg ttcgccacaa atcttgataa acgtctcaga aatctgaccg
     5881 cttgcaagtg atgagttaag aaaactagct acttgctcat cgttagcgac ggcggcagaa
     5941 aaagttaaca tctgctgcca ttgttccaat tgaccttgtt ctaaagcaaa atcgaaagcc
     6001 gctttagcat aggggcgagc tactgtgctt aattctgaca tctgcccctc ctagttatag
     6061 ttccgcaact agcttatcaa taatatcgtt gttcgcagcg gtatccaccg aacgacccac
     6121 gattttttct gcaccagcaa tagccaatgc agccactttt tgacgaagct cttcttgaac
     6181 gcgtttacgt tcgctttcaa cttccgcgta accctgttca ataatacgtt gacgttccac
     6241 ttcagcttct gcttgtacag attctaaaat ttcattacga cgttttgttg ctaaatcaat
     6301 gatattttgt gcttcttctt tcgctctaag gatttcttga tccgcagccg ctttagaatc
     6361 agcttgttct tgtttcgctt tttcggcaga agccaatgcg ctagcaatat ttgcctgacg
     6421 ctcttctatc gctcgaatta gcggtggcca tacaaacttc atacagaacg caacaaaaag
     6481 tgcgaacgca atcagttgac caattagtgt tgcatttaaa ttcacaacgc cctcctaaaa
     6541 gccggtaaag ttattcaact gaataacggt taatcaaatc ccgacttatt tcaataaatc
     6601 aatgaacggg tttgcgaaga tgaatagtaa tgaaatacct acagcgatca ttgcgatagc
     6661 atctaaaaga ccagcaacga taaacatttt agtttgtaag cttgatgcta attccggctg
     6721 acgtgcagat gactctaaga atttgccacc taagatagca aagccgatag cagtaccaag
     6781 agcagcgaaa gctaataaaa ttgaagcacc aataattgtt gctgtgatta ctgattccat
     6841 aatgttctcc attagattga ggaattagcc cgaaggctgg gttgttgata aaaatacata
     6901 tcaagcggtc aatattttgc aaaaatttgc gaaaaaccta ccgcttgtaa ggctagttta
     6961 atgatccgct ttgttataag cgatactcaa ataaacaatc gttagcatca taaaaataaa
     7021 ggcttgaagc gtaataacta aaatatggaa aatcgcccaa accaagtgta atggaatccc
     7081 caacgcctgt agagcgaagt tgtctgccat atacattact gcaataagga taaagattaa
     7141 ctcacctgca tacatattac cgaataaacg gaacgccaat gaaatcggtt ttgccaaaag
     7201 cgttaccgtt tcaagaataa agtttaccgg aatgaatgcc cagtggttaa acgggtgtaa
     7261 cgtgtattct ttcactaaac cgccaaaacc tttcgatttc accgtgtaaa ataaaattaa
     7321 cgcaaataca caaatagaca tacccagtgt tgcactaata tctgccgttg gtactgcacg
     7381 caaatagtga ataccaaaca gattggctaa ctgtggtaag aaatcgaccg ggataaggtc
     7441 gatcgcattc ataataaaca cccaacagaa aatcgttaat gctaatggtg caattacatt
     7501 gcgaggtccg tggaagttat ccttcactaa accgtttacc cactcaataa caatctcaac
     7561 aaaacattgc aattttcccg gaacgcccgt tgttgcattt tttgctacac gcgagaacac
     7621 ccataaaaaa atcgcacctg ccactaaaga gaagaacagt gtatctaaat gcactgccca
     7681 aaagccttca cctgtcgata aaaaactcaa gtgatggcta atatattctg ctgtcgttcc
     7741 agccataaca tatcctttta aattaaaatt agtggtgatg tttgcttaat acaaacggga
     7801 tcatattatt taaaaataat gcgataaaat aacccacgaa aaacaacaca acatctagag
     7861 cagggataag tttaaaacag ccgatcacca aaataatagt aaccaaccat ttcattcctt
     7921 caccacgata gaaagcggtc atttttgact gattttttgc agatttctta aagaaaaccc
     7981 aatatacaaa cacaagatga ggcaaaaaac tcgctaaagc accgcctaga aaggaaaaat
     8041 taaaattcat tttccataaa gacactccaa gtgcaaaaac gcacaagaga aaaaattcaa
     8101 cctttaatgc ttgtttatat tgtgattttg ctctattgat tacagctgac acattttatc
     8161 ctttttgaat aagtaaacca cacaatcagg ctaataatta gccactgttg aatcaaagaa
     8221 aatccgtgct aaattatact ttacaaatg
//
LOCUS       DANJWC010000111         8232 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.111, whole genome shotgun sequence.
ACCESSION   DANJWC010000111 DANJWC010000000
VERSION     DANJWC010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8232
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.111"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(3..713)
                     /locus_tag="PWX02_001747"
     CDS             complement(3..713)
                     /locus_tag="PWX02_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL1729741.1"
                     /translation="MWKNRQLFFGYAENRLLLLSRSITGLLGILCGVYIIDHLVLSDV
                     DMIGKLTSFILIILSAIFLKERASLLQWILCLIAFIGALFIIKPAVDIRFMPYLVGVI
                     GSVFAAIAYLCLRLLAKTKKVESPNTIVFFFSAFSTLILLPFVAIDYVVLTNLQTIYL
                     VLAGVTSAVGQFCVTTAYKYAAAKDISIYSYASVLFSAILGLLFFEQYPDMLSWLGYA
                     IIFVSSWLMFVLTKKQSK"
     gene            complement(728..880)
                     /locus_tag="PWX02_001748"
     CDS             complement(728..880)
                     /locus_tag="PWX02_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729742.1"
                     /translation="MNFWNSLSPLFKAIFCVLLSALSFAIMAAFLRLSGDVPIAQKAI
                     FRNAQH"
     gene            complement(949..1434)
                     /locus_tag="PWX02_001749"
     CDS             complement(949..1434)
                     /locus_tag="PWX02_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDL1729743.1"
                     /translation="MKKFAKVLMVAVPAFALAACSSSSDDAAANAAANAAANAAANAA
                     GEFGGLTAEQLKAQYNTVYFGFDSYAVEGEYRQLLDAHAQLLAKTQGNVTIAGHADER
                     GTPEYNIALGQRRADAVKGYLAAKGANNTSTVSYGEEKPAVLGHSEADYAKNRRAVLE
                     Y"
     gene            complement(1461..2747)
                     /gene="tolB"
                     /locus_tag="PWX02_001750"
     CDS             complement(1461..2747)
                     /gene="tolB"
                     /locus_tag="PWX02_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HDL1729744.1"
                     /translation="MKLFSRISGVIGIFSALLTTQVVADSDVRISIDEGVSMAQPIAV
                     VPFKASGVPADVAQIISDDLRNSGKFTPLERSQMPAQPGSAAEVVADQWSAIGIDNIV
                     VGQVSSTGGGYNVAYQLIDTLGTSGTAGGVIAQGSFIVPAAQIRQGAHTVSDEVFEKL
                     TQIRGAFRTKIAYVVQRGVSSYELRVSDYDGFNAFTITTSKEPLMSPEWSPDGSRLAY
                     VTFENKKAQVVVHDIRSGARRVVAALKGHNGAPSFSPDGSRIAFASNQDGELNIYVVG
                     SGGGTPSKLTANAGNNTEPSWSPDGSTIYFTSDRAGSPQVYRMSSSGGGASPMGGSGS
                     YNAKVSSDGKNLIMIAGDRVVKRDLASGGTEVLSSTFLDESPSISPNGIMVIYSSTKG
                     TSKVLQLVSADGRFKANLPGAGGHYKFPAWSPYLTK"
     gene            complement(2763..3986)
                     /gene="tolA"
                     /locus_tag="PWX02_001751"
     CDS             complement(2763..3986)
                     /gene="tolA"
                     /locus_tag="PWX02_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDL1729745.1"
                     /translation="MKSQQDRLELAVIVSILLHILLIGLLLLGSLFTKTELAAAGGSG
                     GDSDSFEAVMVDTGQVAAEYGQLLAEKKGSKASKPKVEPKEEAVEEDVEDKPTPEEIQ
                     KQKQAELAAIQEKQRQQEIERQQKLQEQQKQAQAKQEELKKQQAEEATRKKAAEAAKL
                     KADAEAKRLEAAAKQAEEEKKAKEALEKKLEQQKKAEAEKKLKEQKELKEQQAKEAKE
                     AKEKAEKEAKAKAEKEAKEKAEKEAKAKAEKEAKEKAEKEAKAKAAAEAKAAQAKNNK
                     ALDDFLNSGDIGGGSSKGGNKNSAGSQGSGGTSGLGQGTNVDGNAYGQRIKKLIQSRY
                     RVDPSFAGKQCDVKIFLSRDGTITNYQVISGDKAVCDAAVSAIVATRKVPPAPSDAVY
                     NMFKSPTLDFSLKVK"
     gene            complement(4000..4437)
                     /gene="tolR"
                     /locus_tag="PWX02_001752"
     CDS             complement(4000..4437)
                     /gene="tolR"
                     /locus_tag="PWX02_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HDL1729746.1"
                     /translation="MSRRLKRNDIKSEINIVPFLDVLLVLLLIFMATAPIISQSVEVN
                     LPEDTHSQSVSNEDKTPVILEVAGVGEYKLKIDGNYVQSNGQENINESEVVAYAGEQF
                     QKDNNTLFLVAGDKVVPYEEIMKGITLLKDAGVKSVGLMTEGK"
     gene            complement(4579..5259)
                     /gene="tolQ"
                     /locus_tag="PWX02_001753"
     CDS             complement(4579..5259)
                     /gene="tolQ"
                     /locus_tag="PWX02_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HDL1729747.1"
                     /translation="MQTEFSLLSLFLEASIVVKIVMLILIVFSILSWAVIIQRSKILT
                     KAKKDSLAFEDRFWSGEDLNRLHQGLENRRDALNGSEQIFYVGFKEFTRLQQANPDAP
                     ESIIQGSTRAMNLALNREMESLENYIPFLGTVGSISPYIGLFGTVWGIMHSFMGLSAV
                     KQATLQSVAPGIAEALIATAIGLFAAIPAVMAYNRLNLRLGKVEQGYVNFIDEFTTLL
                     HRQAFSKR"
     gene            complement(5281..5682)
                     /gene="ybgC"
                     /locus_tag="PWX02_001754"
     CDS             complement(5281..5682)
                     /gene="ybgC"
                     /locus_tag="PWX02_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDL1729748.1"
                     /translation="MDFPIRVYYEDTDAGGVVYHSNYLNFFERARTEFLRQQHFSQQE
                     LFSQSLAFVVKRIEIDYKLPARLDDLLNVETTLSELKKATLIFKQRLWKDGDCLSEAN
                     VTVACVDLIKMKPVAIPDDIRQALQKSYFSI"
     gene            complement(5845..6132)
                     /gene="ybgE"
                     /locus_tag="PWX02_001755"
     CDS             complement(5845..6132)
                     /gene="ybgE"
                     /locus_tag="PWX02_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HDL1729749.1"
                     /translation="MIHKLYNLTAKGLLKALSFILAAVLFATIWVNSTAFALSFGGKT
                     PYLAMLVFYGMAILWVHGIGFEIRAAIWKVIFLPLLGYLIVIPALWILLVK"
     gene            complement(6132..6230)
                     /locus_tag="PWX02_001756"
     CDS             complement(6132..6230)
                     /locus_tag="PWX02_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CydX/CbdX family cytochrome bd oxidase small
                     subunit"
                     /protein_id="HDL1729750.1"
                     /translation="MFYVTWVLGVLLAIFFATVITISIEKTGKFDE"
     gene            complement(6245..7378)
                     /gene="cydB"
                     /locus_tag="PWX02_001757"
     CDS             complement(6245..7378)
                     /gene="cydB"
                     /locus_tag="PWX02_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDL1729751.1"
                     /translation="MIDYEILRFIWWILIAVLLIGFAITDGFDMGVLNLLPIIGKSNV
                     ERRIMINTIAPHWDGNQVWLLTAGGAIFAAWPTVYATSFSGFYIAMVLVLAALFFRPV
                     GFEYRAKIDNPKWRSAWDWGLFIGGFVPSLVFGVAFGNLLQGVPFEFNNINQSQYTGS
                     FFGLLNPFALVCGIISLMMLTTQGATWLQMKTTDELHNNSRKVAQVTASIVLVAFILA
                     GVWLYFKDGFVITSELDHNAQSLLTNKTVAVQAGAWFRNYAEMPVLWVVPALVVVGAL
                     LTIIASKANRSGLAFFSSSLMIVGVILTAAVSMFPFIMPSITHPEMSLTVWDATASHT
                     TLTVMLFVAGIFVPLVLAYTIWSYIKMYGRLDASYLEENKAAY"
     gene            complement(7391..>8232)
                     /locus_tag="PWX02_001758"
     CDS             complement(7391..>8232)
                     /locus_tag="PWX02_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDL1729752.1"
                     /translation="GDESGYEIGKAQPTKLAAMEGEFETQPAPAAWNAFVIPNTAEMK
                     NEFSIHIPYAAGIIATRSLDKEIKGLKDIRVINEQRVRTGMVAYGLLEKLRSGNYTEA
                     DKEAFKAVQKDLGFGLLLKQYTPNVVDATEEQIQKAADNTIPNVGPTFWAFRVMMLSG
                     FLMLILITTAFIQNMRGKVESSRFLLRGLLFGLPLPWIAIESGWFLAEYGRQPWAIYE
                     MLPVGVSNSALTTGDLWFTIGLLCGLYTLFLVVEMYLMFKYGRLGPSALKTGRYYFEQ
                     STK"
BASE COUNT         2544 a         1814 c         1370 g         2504 t
ORIGIN      
        1 ttttactttg attgcttttt agttaataca aacataagcc agcttgagac aaaaataatt
       61 gcataaccta gccaactcaa catatccggg tattgttcaa agaacaacaa ccctaaaatt
      121 gcactaaata gcacagaggc ataactataa attgagatgt ctttcgccgc cgcatattta
      181 taggcggtag ttacacaaaa ctgcccgacc gctgaagtta cccctgctaa cactaagtag
      241 attgtctgta aattggttag cactacataa tcaatggcga caaaaggcaa taatatcaag
      301 gtcgaaaagg cagaaaaaaa gaaaacaatg gtattaggtg attctacttt tttggttttt
      361 gccaataacc ttaagcataa ataggcaatg gctgcaaaca cagagccaat cacgccaacc
      421 agataaggca taaagcgaat atcaactgcc ggtttgataa taaaaagtgc cccgataaag
      481 gcaataaggc ataaaatcca ttgcaggaga gatgctctct cttttaaaaa aatcgcagaa
      541 agaataatta aaatgaacga cgtgagtttg ccaatcatat cgacatcact tagcacaagg
      601 tgatcgatga tatatacacc acataaaatc cccaataacc cggtgatgga acggcttaat
      661 aataacaaac gattttctgc ataaccaaag aataattggc gatttttcca cacaatataa
      721 ccgcttatca atgctgtgcg ttgcgaaaaa tcgctttttg tgcaatcggt acatcccccg
      781 aaagacgaag aaaagcagcc ataatcgcaa agcttagtgc cgacaaaagc acacaaaaaa
      841 tggctttaaa taacggcgag agcgaattcc aaaaattcat agcataaagg cttttaataa
      901 aaataaagcc ctgattattc agggcttcaa tttagaatca actaagaatt agtattctaa
      961 tactgcacga cggtttttcg cgtagtcagc ttctgagtga cctaatactg ctggtttctc
     1021 ttcaccgtaa gaaacagttg aagtgttgtt agcaccttta gctgctaagt aacctttaac
     1081 tgcatctgcg cgacgttgac ctaatgcgat gttgtactct ggagtaccac gctcatcagc
     1141 gtgaccagca atagttacgt taccttgagt ttttgctaat aattgagcgt gagcgtctaa
     1201 taattgacgg tattcacctt caactgcata gctgtcaaaa ccgaagtata cagtgttgta
     1261 ttgtgctttt aattgttcag ctgttaaacc accgaattcg cctgctgcat ttgctgctgc
     1321 atttgctgct gcgtttgctg ctgcgtttgc tgctgcatca tctgatgagc tgctacatgc
     1381 tgctaatgcg aaagctggta ctgctaccat taatacttta gcaaattttt tcatttgttg
     1441 ctcctaatag agaaatatta ttatttagtt aagtatggtg accatgcagg gaatttataa
     1501 tgtccacctg cgccaggcaa attagcttta aaacgcccat ctgcagacac caattgtagc
     1561 actttactcg tacctttggt agagctataa ataaccataa tgccattcgg tgaaatactt
     1621 ggactttcgt ctaagaaagt tgaactcagt acttctgtgc cacctgaagc gagatcgcgt
     1681 ttcacaactc tatcaccggc aatcatgatt aaattcttac catcagatga tactttcgca
     1741 ttatagctac cgctaccgcc cattgggcta gcaccaccac ctgatgaact cattctataa
     1801 acttgaggag aacctgcacg gtcagacgta aagtagattg tgctaccatc tggagaccag
     1861 cttggttcag tattatttcc agcattagcg gtcaatttac ttggtgtacc accgccacta
     1921 cctacaacat aaatatttaa ctcaccatct tgatttgagg caaacgcaat gcgagaaccg
     1981 tctggagaga atgatggcgc accattatgt cctttcaatg ctgccactac acggcgagca
     2041 cctgaacgaa tatcgtgaac aacaacctgc gcttttttat tttcaaatgt cacataagct
     2101 aatctgctac catctggtga ccactctggc gacattaaag gctctttact ggttgtaata
     2161 gtaaaggcat taaaaccatc ataatcagat actcgtaatt catacgatga tacgccacgt
     2221 tgaaccacat acgcaatttt agttctaaat gcaccgcgaa tttgggttaa tttttcaaat
     2281 acctcatcac ttactgtatg cgcaccttga cgaatttggg ctgccggaac aataaatgaa
     2341 ccttgggcaa tgacaccacc cgctgtaccc gatgtaccaa gagtatcaat taattgataa
     2401 gcgacattat aaccgccacc tgtgctactc acttggccaa ctacaatatt atcaataccg
     2461 atagcactcc attgatctgc caccacttcc gctgctgatc ccggttgagc aggcatttga
     2521 gagcgttcaa gcggtgtaaa tttaccacta tttcgcaaat catcagaaat aatttgtgca
     2581 acatctgccg gcacaccgct ggctttaaat ggcacaaccg caatcggctg agccatactt
     2641 acaccttcat caatagaaat tcgcacatca gaatcagcaa ccacctgagt tgtcaataat
     2701 gctgaaaaga tgcctattac acccgatata cgagagaata atttcatttg tagtcctcta
     2761 aattatttaa cttttaaact aaaatcaagt gttggtgatt taaacatatt atataccgca
     2821 tctgatggtg caggtggtac ttttctagtt gccactattg cacttaccgc tgcatcacat
     2881 actgctttat cgccactgat aacttgatag ttagtaattg tgccatctcg acttaagaat
     2941 atcttaacat cacattgttt tccggcaaaa cttggatcaa cccgatatcg agattgaatt
     3001 aatttcttaa ttcgttgacc atatgcatta ccatctacat tagtaccttg ccctaagcca
     3061 cttgtaccac cagaaccttg tgatcctgct gagtttttgt taccaccttt acttgaacca
     3121 ccaccgatgt ctccactatt gaggaaatca tcaagtgctt tattgttttt cgcttgggcg
     3181 gcttttgcct cagcagctgc cttcgcttta gcttctttct cggctttctc tttggcttct
     3241 ttttctgctt ttgctttggc ttctttctca gccttttctt ttgcttcttt ttctgccttg
     3301 gctttagctt ctttttctgc tttttccttc gcttctttag cttcttttgc ctgctgttcc
     3361 ttcagttctt tttgctcttt taactttttc tcagcttccg ctttcttctg ctgttcaagc
     3421 ttcttttcaa gtgcttcttt agcttttttc tcttcttcag cttgtttagc agcagcctct
     3481 aaacgcttag cttctgcatc tgctttcagt tttgctgctt cagctgcttt tttacgagtt
     3541 gcttcttctg cttgctgctt tttaagctct tcttgcttag cttgtgcttg cttttgctgt
     3601 tcttgtagtt tttgctgacg ctcaatttct tgctgacgct gtttctcttg aatcgccgct
     3661 aactcagctt gtttctgttt ttggatctct tccggagtcg gtttatcttc cacatcttct
     3721 tcaacagctt cctctttagg ctctaccttt ggcttcgatg cttttgagcc ttttttctct
     3781 gccaataact gcccatattc agcagccact tgcccagtat ctaccatcac agcttcaaag
     3841 ctatcgctat cgccaccact gccaccagcc gcagcgagtt ccgtcttagt gaaaagagac
     3901 ccgagcaata acaacccgat taacaatata tgcaataaga tcgaaacaat aaccgctagc
     3961 tctagtctat cttgttgaga tttcacacgt tatccttggc tatttacctt ctgtcattaa
     4021 acctaccgat ttcactccgg cgtctttaag tagtgtaata cctttcataa tttcttcata
     4081 aggtactact ttatcaccgg caactaaaaa taaggtattg ttatcttttt gaaattgttc
     4141 ccctgcatag gcaacaactt ctgattcatt aatattctct tgcccattac tttgcacata
     4201 gttaccgtca attttcaatt tatattcacc aacacctgcc acctccaaaa tcactggagt
     4261 tttatcttca ttagaaactg attggctatg cgtatcttcc ggtaaattaa cttctacact
     4321 ttgactgata attggggccg ttgccataaa aatcagcaat aataccaaca atacatcaag
     4381 aaatggaaca atgttgattt ctgatttaat gtcattacgt tttaaacgac gagacatata
     4441 ttcctcttta caggtgctta gcaccttaat tgatctgaaa taaccggcac agtgtcccgg
     4501 tctcgaataa tgcggttaca agcggtcttt tttacgaaaa actttacaaa ttttcattaa
     4561 aaatcaaccg cttgtagatt atctttttga aaaagcctga cggtgtaata aggtcgtgaa
     4621 ctcatcaatg aaattcacat acccttgctc aactttacct aaacgtaaat tcaaacggtt
     4681 atatgccatt actgcaggaa tcgccgcaaa taaaccgata gccgttgcaa ttaatgcttc
     4741 tgcaataccc ggtgcgactg attgtaaagt tgcttgctta actgcactta atcccataaa
     4801 ggagtgcata attccccaaa ccgttccgaa taaaccaata tatgggctga ttgaacccac
     4861 tgtgcctaaa aacggaatgt agttttctaa actttccatt tcacggttta atgctaagtt
     4921 catcgcacgg gtagaacctt gaataatcga ttctggcgca tctgggttcg cttgttgtaa
     4981 acgggtaaat tctttaaacc caacataaaa gatttgctct gaaccattta atgcatcacg
     5041 acgattctct aaaccttggt gtaaacggtt tagatcttcc cctgaccaga atcgatcttc
     5101 aaaagcaagg gaatcttttt tggctttagt taaaattttg ctgcgctgaa taatcaccgc
     5161 ccaagacaaa attgagaata caataaggat cagcatcacg atctttacca caatacttgc
     5221 ttctaaaaat aacgaaagaa gactaaattc agtttgcatt aataaaactc cgaatagttt
     5281 ttaaatagaa aaataacttt tttgtaatgc ttgacgaata tcatcaggaa ttgcaaccgg
     5341 cttcattttg attaaatcaa cacaagcgac cgtaacattt gcctcactca gacaatcgcc
     5401 atctttccac aatcgttgtt taaagataag ggttgctttc ttcaattctg agagtgttgt
     5461 ttccacattt aataaatcat ctaaacgagc agggagctta taatctattt caatcctctt
     5521 aacaacaaaa gcaagggatt ggctaaaaag ttcctgctgt gagaaatgct gctgtctcaa
     5581 aaattccgtt cttgcccgct caaaaaaatt cagatagttg gaatgataaa ctactccgcc
     5641 ggcatcagta tcttcataat aaacacgaat cgggaaatcc ataagcatta tttctctcat
     5701 tcaaaatgaa aaaacgccca aaatttaggc gcaaattgtg agatatttta ggatcagaaa
     5761 tattgttatg caagtatatt ttttggtaaa ttgcaaaaat attttttatc tatcaaaaat
     5821 aaaaatagag acataaaaat gcctctattt aacaagtaaa atccataatg ccggaataac
     5881 aataaggtaa cctagaagtg gtaaaaaaat cactttccaa atagcagctc ggatttcaaa
     5941 tccaattcca tgtacccata aaatagccat accataaaat accaacattg ctaaatacgg
     6001 cgtttttcca ccaaaagaaa gagcgaaagc ggtcgaattt acccaaatag ttgcaaataa
     6061 tacagccgct aaaataaaag aaagggcttt taacaagccc tttgctgtta agttatagag
     6121 tttatgaatc attactcgtc aaacttacct gttttttcaa tgctgatcgt tatgactgtt
     6181 gcaaagaaga ttgcaagcaa tacacctaag acccaagtta cataaaacat attgtgtctc
     6241 cttattagta agctgcttta ttttcttcta aataactagc atctaaacga ccatacattt
     6301 tgatatatga ccaaatagta tatgctaata ctaacggtac aaagatacca gcaacaaata
     6361 acattacagt taatgttgtg tggctagcag tagcgtccca aaccgttaag ctcatttctg
     6421 gatgagtaat tgacggcata atgaatggga acattgatac tgcagcagtt aaaatcaccc
     6481 caacaatcat caatgatgat gagaagaatg ctaaacctga acgatttgct tttgaggcaa
     6541 taatagtcag taatgcacct actacaacta atgcaggaac aacccataat acaggcattt
     6601 cagcataatt tctaaaccat gcaccagctt gaaccgctac agttttattc gtcaatagag
     6661 attgtgcatt atgatctaac tcgcttgtga tcacaaaacc atctttaaaa tataaccata
     6721 cacctgcaag aataaatgcc actaatacaa tagaagctgt cacttgtgcc acttttcttg
     6781 agttgttatg tagctcatca gtagttttca tttgtaacca agttgcaccc tgtgtggtta
     6841 acatcattaa gctgataata ccgcacacta atgcaaatgg atttaataaa ccaaagaatg
     6901 aacccgtata ttgtgattgg ttaatattat taaattcaaa tggtacacct tgtaataagt
     6961 taccgaatgc aacaccgaat actaatgatg gaacgaaacc accaataaat aatccccaat
     7021 cccaagcact acgccatttc ggattatcaa tttttgcacg atactcaaaa cctacagggc
     7081 ggaaaaataa tgctgcaagc acaagcacca ttgcaatata gaaacccgag aacgaagttg
     7141 catagactgt tggccatgca gcgaagatcg caccaccagc ggtcaataac catacttggt
     7201 taccatccca gtgaggggca attgtgttga tcataatacg acgttcaaca ttacttttac
     7261 caatgatagg taaaaggttt aatacgccca tatcaaaacc atcagtaatt gcaaagccaa
     7321 ttaacaatac cgcaattaag atccaccaaa taaagcggag aatttcataa tcgatcattt
     7381 ctaactccta ttatttagtt gattgttcaa aataataacg gcctgttttt aatgcactag
     7441 ggccaagacg accatatttg aacattaaat acatttctac aactaagaat agtgtatata
     7501 aaccacacaa taagccgatt gtaaaccata aatcacctgt tgttaatgca gagtttgaca
     7561 caccgacagg taacatttca tagatagccc aaggctgacg gccatattcg gcaaggaacc
     7621 aaccagattc aatcgcaatc caaggtaacg gaagaccgaa taacaagcct cttaataaga
     7681 aacggctact ttctacttta ccacgcatat tttgaataaa tgctgtcgta atcaagataa
     7741 gcatcaagaa gccagataac atcattacac ggaatgccca gaatgtaggg cctacatttg
     7801 gaattgtatt atctgctgct ttttgaattt gttcttcagt cgcatcaacc acattaggtg
     7861 tatattgttt taacaataac ccaaagccta aatctttttg tacagcttta aatgcttctt
     7921 tatccgcctc tgtataattg ccagaacgta atttttctaa tagtccgtag gctaccatac
     7981 cggtacgaac acgttgttca ttaattacac gaatatcttt taggccttta atttctttat
     8041 ctaaagaacg ggttgcaata ataccagctg cataaggaat atgaattgag aattcatttt
     8101 tcatttccgc agtatttgga atcacaaaag cattccaagc agctggagca ggctgagttt
     8161 cgaattcacc ttccattgca gctaatttag tcggctgagc tttaccgatt tcataaccag
     8221 attcatcacc ca
//
LOCUS       DANJWC010000112         8151 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.112, whole genome shotgun sequence.
ACCESSION   DANJWC010000112 DANJWC010000000
VERSION     DANJWC010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8151
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.112"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            73..2004
                     /locus_tag="PWX02_001759"
     CDS             73..2004
                     /locus_tag="PWX02_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1729753.1"
                     /translation="MIFFTDLILKRGHSVLIDNSSVTIHTGQKVGLVGKNGCGKSSLF
                     ALLKNELQPEGGDVSLPKNWAISWVNQETPALAISALDYVIQGDREYTHLMAELDIAN
                     EKNDGNRIAIIHTQLDTIDAWTIQSRAATLLNGLGFSTEQLQLPVKSFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLERWLSNYRGTLLLISHDRDFLDPIIDRVIH
                     IEHQKLNEYTGNYTSFEIQRATKIAQQNAAYQQQQRKVAHLQSFIDRFKAKATKAKQA
                     QSRIKALEKMELIAPAYADSPFEFEFRPPLALPSPLLMMEKVSAGYAEKTVLESVKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELPPQNGSIQLAKGVQLGYFAQHQVDTLRFDE
                     SPLWHLQKLAPEKTEQELRNYLGGFDFKGDKVVQAVKSFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLEMRQALVDALTYYEGSLVVVSHDRHLLRSTVNEFYLVHDRKVEKF
                     KGDLDDYQKWLNEQNALANEPKSVLAKDSANSTACSSTNENSAAQRKEQKRLEAERRQ
                     QTAPLRKKITQLEKELEKATEKLNKLEAMLTESDIYEAENKAKLTETLAKQLEAKRLV
                     EEIEMQWLEAQEEFETMLR"
     gene            2283..2540
                     /locus_tag="PWX02_001760"
     CDS             2283..2540
                     /locus_tag="PWX02_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1729754.1"
                     /translation="MKNLRLSIHSSEHIWLRELFLKRRLELGLTQRTLGEKMGVLYSF
                     IGKVETGDRRLDIFEFIAYCKGLDLDPLSVLQEIEQQFPHK"
     gene            complement(2587..3207)
                     /locus_tag="PWX02_001761"
     CDS             complement(2587..3207)
                     /locus_tag="PWX02_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HDL1729755.1"
                     /translation="MKLWYSTTSPFARKALAVIKHHQLDDKVEMLRVTKAMDPNSPHN
                     QDNPLGRIPALQRNCGRWLFGSLLISEYLDQKGNNTPLLPKEGKPRWAVLALHNLADG
                     IMENTMPTLLQERMARPENEWWVSRHEQLMERNIRSFRQLEEALQEFGTELNLGTLTA
                     VALIDWWAFRLDKIGYDLAKNYPNLTAWAEEMNNKYAILAETKPSM"
     gene            complement(3209..3862)
                     /locus_tag="PWX02_001762"
     CDS             complement(3209..3862)
                     /locus_tag="PWX02_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HDL1729756.1"
                     /translation="MTPTLPICYQHRDFIIINKPEGLSVHKDEAEIGLTELVARQLNV
                     KQVWLVHRLDKITSGLLILALNKEAAARFYHLFEQHKIYKTYWALSDKKPKKKQGRIV
                     GDMQKSRNGAWKLCHSKENPAVTQFISHSVEDSLRHFILQPKTGKTHQLRVAMKSLGS
                     PILGDKLYSGSQADRVYLHAYQLDFEYENEKISVQALPTSGHFWQKICKSNHIKHGE"
     gene            3911..4570
                     /locus_tag="PWX02_001763"
     CDS             3911..4570
                     /locus_tag="PWX02_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL1729757.1"
                     /translation="MAVTIYLVRHGRTVWNLEGRLQGSGDSALVEEGIIGAKKTGIAL
                     KHIPFTAAYSSMQKRAQDTANYILAENERSNIPHFHHKGLNEFDFGSWEGMKSVDLQE
                     NDEYWVMKRTPAEYLAKANGGERFEQLYQRVTQAFNQIAELHQNDGKVLIVSHGMTLT
                     LLTAVLKGIAWQDFRNEEKHSFVFNTAITKVEVENGKVTVLEFNNTDHLDNVPTSQYK
                     R"
     gene            complement(4607..5653)
                     /locus_tag="PWX02_001764"
     CDS             complement(4607..5653)
                     /locus_tag="PWX02_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HDL1729758.1"
                     /translation="MSKQIFNEEELKTTEHFEPKQEFDLENAIIEEEEKAVEAELIIE
                     ESLKPSRFWIRVLLATLALFGVAVIAQSIQWLIDTFQQREWIYFAFSIVFFIVSLLGV
                     GAIISEWRKLVYLRNHQKNQQMSNQLLLEQLPSNDGEQAVKFCENIANQLNHLPSIAQ
                     AEQLWKSELNEAYNAKEVLYLFSENVLKPIDKQVKQMISRNAAENAVIVAVSPLAIVD
                     VLLMAARNIALVNKITKAYGMELGYISRLKLFKMVLKNMVFAGATEIATDVGMDFFSQ
                     NLTAKLSLRAAQGIGVGLLTARLGIKAMEFCRPVAFQANEKPRISAIRQELLTSVKNT
                     VFAKTETKEKVFSD"
     gene            complement(5653..6606)
                     /gene="dusC"
                     /locus_tag="PWX02_001765"
     CDS             complement(5653..6606)
                     /gene="dusC"
                     /locus_tag="PWX02_001765"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HDL1729759.1"
                     /translation="MNPIPNVILAPMQGVLDPFVRNLLTSVNDYDLCISEFVRVVDQR
                     LPKKTFYRLAPELYQGGLTDSGTPVRVQLLGQHPQWLAENAALAIELGSHGVDLNCGC
                     PSKTVNGSNGGASLLKDPELIYRATKAMREAVPSDKIVSVKVRLGWDSVSQCFEIADA
                     VEQGGANEITVHGRTKVDGYRAERINWQAIGEIQQRLSIPVIANGEIWDFESAKNCQK
                     ITACKALMMGRGALNTPNLSKVVKYNVPKMAWNEVLQLLFKYVQMENQFDTGFYHVAR
                     IKQWLRYLDKEYPQAVELFDILKTEHGYDGLKAHIEKAVEE"
     gene            complement(6684..7310)
                     /locus_tag="PWX02_001766"
     CDS             complement(6684..7310)
                     /locus_tag="PWX02_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HDL1729760.1"
                     /translation="MDSRSHIIPLFLAIVITVIAVWSGLNPTDRAVWYAEVIPIFIVF
                     ALLIITYPKFQFSGLAYFLMSLWLIMHLIGAKYTFANVPFEWANQFLTPILGEDRNHF
                     DRVAHYIIGFYSFPMAEWLLRKRKCGMGVAFFFSLFFIMSVAAAYEIIEWQYAVIEGG
                     NAGVEFLGSQGDIWDAQKDMLADTLGALTALMIYLIARPDLRIHDRHF"
     gene            complement(7310..8149)
                     /gene="djlA"
                     /locus_tag="PWX02_001767"
     CDS             complement(7310..8149)
                     /gene="djlA"
                     /locus_tag="PWX02_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HDL1729761.1"
                     /translation="MVGFFLGYQLFHGLFGGILGAFIGHLADKKLYELGSVRSSLFGK
                     NLTRQSLFTQTTFAVLGHIAKAKGRVTEADIELARALMARLKLDSAAQQLAQNAFTLG
                     KEPDFPLRQVIQEFREACGARTDLLRFFVEVQMQAALQDGQLDVNEQQILFTIAETMG
                     MSRFQFEQMIAMIMAAQQFRNGGYYQQNGSYHQQSQGGYQGSYQQPAQPSIEAAYTVL
                     GVTAQDDQNTVKRAYRKLMNEHHPDKLAAKGLPDEMMELAKEKAQQIQAAYDLICKVK
                     EWK"
BASE COUNT         2406 a         1798 c         1643 g         2304 t
ORIGIN      
        1 agttcacaaa gcaatggctt tgctttttta tgctaaaata cgcaaaattt ttaacaaaat
       61 agaccgcttg caatgatctt ttttacagac ttaattttaa aacgagggca ttccgttctc
      121 attgataaca gctccgttac cattcacacc gggcagaaag tcggtttggt gggaaaaaac
      181 ggctgtggca aatcctctct ttttgcctta ctgaaaaatg aattacaacc tgaaggtggc
      241 gatgtatcgt tgccgaaaaa ctgggcgatc tcgtgggtaa atcaagaaac gcctgcgtta
      301 gctatttcag cgttagacta tgtgattcaa ggagatcggg aatataccca tcttatggct
      361 gagctagata tcgcaaatga aaagaatgac ggcaaccgca ttgcgattat ccatactcaa
      421 cttgatacca ttgatgcttg gacaattcaa tcccgtgccg ctaccctgct taacggcttg
      481 gggtttagca ccgaacaact gcaactgccg gtgaaatcct tctccggtgg ctggcgtatg
      541 cggttaaatt tggcacaagc tctgatttgt cgttccgatc tgttattgct tgacgagcca
      601 accaaccact tagacttaga tgcggttatt tggttggagc gttggctgag taactatcgt
      661 ggcactttat tgttaatttc gcacgaccgt gattttctcg atccgattat cgatcgggtg
      721 attcatattg aacatcaaaa gctcaatgaa tacaccggca attacacctc gtttgagatt
      781 caacgtgcga ccaaaatcgc ccagcagaat gccgcttatc agcaacagca acgtaaagtt
      841 gctcatttgc agagttttat cgatcgcttt aaagccaaag cgactaaagc gaaacaggca
      901 caaagccgaa ttaaagcgtt ggaaaaaatg gagcttattg ccccagccta tgctgattcg
      961 ccgtttgaat ttgaatttcg cccaccgctt gcactgccta gcccgttatt aatgatggaa
     1021 aaagtcagtg ccggctatgc ggaaaaaacg gtgttggaat cggtcaaact gaatttggtt
     1081 cccggctctc gtattggttt actcgggaga aacggtgcag ggaaatccac gctgattaaa
     1141 ttattggcgg gcgaacttcc gccccaaaac ggctcaattc aactggcaaa aggcgtgcaa
     1201 ctcggctatt tcgcccaaca tcaggtggat acactacgtt ttgatgaaag cccactttgg
     1261 catttacaaa aactcgcacc ggaaaaaacc gagcaggaat tacgcaatta tttaggcggt
     1321 ttcgatttta aaggcgataa agtggtacaa gctgtgaaat cattctccgg cggagaaaag
     1381 gcccgtttag tgcttgcctt aattgtatgg caacgcccga atttactgct attagatgag
     1441 ccaaccaacc atttagattt agaaatgcga caagccctgg ttgatgcact gacctattac
     1501 gaaggctcgc tagtggtggt ttcgcacgac cgccatttat tgcgtagcac cgtgaatgaa
     1561 ttttatttag tgcacgaccg caaagtggaa aaatttaaag gtgatttaga cgattaccaa
     1621 aaatggctca atgagcaaaa cgccttagcc aacgagccga aatccgtact tgcaaaagat
     1681 tcggcaaatt cgaccgcttg ctctagcacc aatgaaaata gtgcagctca acgcaaagag
     1741 caaaaacgct tagaggcaga gcgccgccaa caaaccgccc cattgcggaa aaaaattact
     1801 caactggaaa aagagttaga aaaagcaacc gaaaaattaa ataaactcga agcaatgctt
     1861 accgaaagcg acatttatga agccgaaaat aaagcaaaac taactgaaac acttgcgaaa
     1921 cagttagagg caaaaaggct ggttgaagaa attgaaatgc aatggttgga ggctcaggag
     1981 gagtttgaga ccatgttacg ctagggttct acaagatagc gcaagcgtcc acgcttgtgc
     2041 catttaaggg aactatacac ctcaaaaaaa caatgatcat taagctagct tttttataaa
     2101 tttaaaaacc tatagattaa ataaataatg atattaatag taaaatcacg agtgcaacta
     2161 cactgacgat aataaaatca ggcatactta ttctccctat aaaatgtggt aatgtaacta
     2221 caaattaatt atataatgaa cctaaaaaag gttcaacatt atttgtacct aatataggtg
     2281 caatgaaaaa tcttagatta tcaatacatt ccagcgagca tatttggtta agagaactat
     2341 tcttaaaacg ccgtttggaa ctagggctaa cacaacgcac attaggagaa aaaatgggcg
     2401 ttctttactc atttatcgga aaagtagaaa caggagatcg cagattagat atttttgaat
     2461 ttatcgctta ttgcaaaggg ttagatttag atcctttatc tgtattgcaa gaaattgaac
     2521 aacaatttcc tcataaataa aggcattgag ttataactca atgcctttat tttctatgtc
     2581 aaatctctac atgctcggct tagtctctgc cagaatggcg tatttattat tcatttcctc
     2641 tgcccaagcg gttaaatttg ggtaattttt tgcaagatcg tagcctatct tatctaagcg
     2701 gaatgcccac cagtcaatta atgctactgc ggttaatgtg cctaaattca gctctgtgcc
     2761 aaactcttgc aatgcctctt ctaattgtct gaacgaacgg atattgcgtt ccattaattg
     2821 ctcgtggcgg ctgacccacc attcattctc ggggcgagcc atacgttctt gtaataaagt
     2881 tggcatcgta ttttccataa tgccgtccgc caaattatgt aatgccaaca ctgcccagcg
     2941 aggtttaccc tctttaggta acagtggagt gttattgcct ttttggtcaa gatattcgct
     3001 aattaacaaa ctaccgaaaa gccaacgtcc gcagttgcgt tgtagggcag gaatacgtcc
     3061 taacgggtta tcttgattat gtggcgaatt tggatccatt gcttttgtca cccgcaacat
     3121 ttcgacttta tcgtctaatt ggtggtgttt aatcaccgca agggctttgc gggcgaaagg
     3181 gctggtggtt gagtaccaaa gtttcatact attctccgtg tttgatgtga ttggatttgc
     3241 aaattttttg ccaaaaatga ccgcttgtcg gcaaggcttg cactgagatt ttctcatttt
     3301 cgtattcaaa atcaagctga taagcgtgca aataaacacg gtcggcttgg ctacctgaat
     3361 agagtttatc acctaaaatc gggctgccca aacttttcat tgccactctt aattggtgag
     3421 ttttgcctgt tttgggttgc aaaataaaat gcctgagcga gtcttcaaca gaatgagaaa
     3481 taaattgcgt tactgccggg ttttccttac tgtggcataa tttccacgct ccatttctgc
     3541 ttttttgcat atcgccaaca attctaccct gctttttctt cggcttttta tcgcttaatg
     3601 cccaataggt tttgtagatt ttatgctgtt caaataaatg gtaaaacctt gctgccgcct
     3661 ctttattcaa cgctaaaatc agtaaacctg aggtgatttt atccagacgg tgaaccagcc
     3721 aaacctgctt tacattcagt tggcgagcca ctaattccgt taagccgatt tcagcctcat
     3781 ctttatgcac actcaagcct tccggcttat tgataataat aaaatcacga tgttgataac
     3841 aaataggcaa tgttggggtc attttgctat aatccttcca atttttgact attttagcaa
     3901 ggaaattaca atggctgtta ccatttattt agtacgacac ggcagaaccg tgtggaattt
     3961 ggaaggcaga ttacaaggct cgggcgattc cgctttagta gaagaaggga ttatcggagc
     4021 gaagaaaacc ggcattgcgt taaaacatat tccgtttacc gccgcctatt ccagtatgca
     4081 gaaacgggct caagataccg caaactacat tttagcagaa aatgaacgca gtaatattcc
     4141 gcattttcat cataaaggct tgaatgaatt tgattttggc agctgggaag gaatgaaatc
     4201 tgtcgatttg caagaaaatg atgaatattg ggtgatgaaa cgtacaccgg cagaatatct
     4261 ggcaaaagca aacggtggcg agcgatttga gcaactttat caacgggtga ctcaggcgtt
     4321 caatcaaatc gccgagctac accaaaatga cggcaaagtg ctgattgttt cacatggtat
     4381 gaccttaacc ttactcacgg ctgtgttaaa aggcattgcg tggcaagatt tccgcaatga
     4441 agaaaaacat agctttgtgt tcaatactgc cattacgaaa gtagaagtag agaatggcaa
     4501 agtaacggtg ctggaattta ataataccga ccatttggat aatgtgccaa caagccaata
     4561 caaacgataa tattaagggc ggaacattgt ccgcccatag cattttctaa tcagagaaca
     4621 ctttttcttt cgtttctgtt ttagcaaata ccgtattttt gaccgaagta agcaactcct
     4681 gacgaatagc tgaaattctt ggcttctcat tcgcttgaaa agcaacagga cgacaaaatt
     4741 ccattgcttt aatgccaagg cgagcagtga gtaaacccac accaatacct tgtgcggcac
     4801 ggagcgataa tttcgccgta aggttttgcg agaagaaatc cattccaaca tcagtagcga
     4861 tttcggttgc accggcgaac accatatttt tcagcaccat tttgaacagt tttaagcggc
     4921 tgatataacc caattccatt ccataggctt tggtaatttt attcactaaa gcgatatttc
     4981 ttgccgccat taacaacaca tcaactattg ccagcgggct aaccgccaca atcaccgcat
     5041 tttctgccgc attgcgggaa atcatctgct taacctgttt atctatcggc tttagcacat
     5101 tttcgctaaa cagatatagt acctctttgg cattataagc ctcgttcagt tcgcttttcc
     5161 aaagttgctc ggcttgagca atgctcggca agtgattaag ctgatttgca atattttcgc
     5221 aaaatttgac cgcttgttcg ccatcattgg aaggcagctg ttccagcaat aattggttac
     5281 tcatttgttg atttttctga tgatttcgca aatacaccaa ctttcgccat tcggaaataa
     5341 tcgccccaac gcccaacaag ctgacaataa aaaagactat cgagaacgca aaatagatcc
     5401 actctctttg ctggaacgta tcaatcaacc actgaatact ttgagcaatt accgccacgc
     5461 caaacaaggc aagtgtggca agtagtactc gaatccaaaa gcgggaaggc ttaaggcttt
     5521 cttcaataat cagctccgcc tcaactgctt tttcctcttc ttcaataatc gcattttcga
     5581 gatcaaactc ttgtttaggt tcaaaatgct cggttgtttt tagctcttct tcattaaaaa
     5641 tttgcttact cattattcct ccactgcctt ttcaatatgt gcttttagac catcatagcc
     5701 gtgttcggtt ttcaagatat caaataattc taccgcttga ggatattctt tatccaaata
     5761 acgtagccat tgtttgatgc gagcaacgtg gtaaaaaccg gtatcaaact gattttccat
     5821 ctgaacgtac ttaaacaata gctgtagcac ttcattccac gccattttcg gaacattata
     5881 tttcaccact ttgcttaaat tcggtgtgtt taacgcccca cgccccatca ttaatgcctt
     5941 gcaagcggtt attttttggc aatttttcgc cgattcaaaa tcccaaattt cgccgttggc
     6001 gataacagga atagacagcc gttgttgaat ttcgccaatc gcctgccaat taattctttc
     6061 tgctcgataa ccatcaactt tggttcgccc gtgaacggta atttcattcg ctccgccctg
     6121 ttctaccgca tcggcaattt caaaacattg gctaacggaa tcccaaccta atcgcacttt
     6181 tacggaaacg attttatcgc ttggcacagc ttcacgcatc gctttggttg cacgataaat
     6241 cagttccgga tcttttaata acgaagcccc accattgctg ccatttaccg ttttagaggg
     6301 gcagccacag ttgagatcta ccccgtgtga accaagctca atggcaagag ctgcattttc
     6361 tgccagccac tgtgggtgtt gccctaaaag ctgtacccgc actggtgtgc cggaatcggt
     6421 taatccgcct tgatatagct caggggctag gcgataaaag gttttcttcg gtaaacgttg
     6481 atcgaccact cgcacaaatt cggaaataca gagatcgtag tcattcactg aagttaaaag
     6541 gttgcggaca aaagggtcta aaaccccttg catcggggca agaataacgt taggaattgg
     6601 attcattggt aaaataaaaa atacaagcgg ttattttttc tcaacatttt gcaaaaaatt
     6661 ggcaagaaac aaccgcttga aggttaaaaa tgtctatcgt gaatgcgcaa atcggggcga
     6721 gcgatcaggt aaatcatcag tgcggttaat gccccaagag tatccgcaag catatctttt
     6781 tgagcgtccc aaatatcccc ttgcgagcct agaaattcca cgccggcatt gccaccttca
     6841 atcacggcat attgccattc aataatttcg taagctgccg ccaccgacat aatgaaaaac
     6901 agcgagaaga aaaatgctac gcccattccg cacttgcgtt tacgcagtaa ccattctgcc
     6961 atcgggaagc tgtaaaaacc gataatgtaa tgtgctactc gatcaaaatg gttacgatct
     7021 tcacctaaaa tcggggttag aaattgattt gcccactcaa acggcacatt ggcaaaggta
     7081 tattttgccc cgatcaagtg cataatcagc caaagcgaca tcaaaaaata agctaaccca
     7141 ctaaactgaa atttagggta agtgataatt aaaagggcaa aaacgataaa aatcggtatc
     7201 acttccgcat accaaactgc gcggtctgtc ggatttaacc ctgaccaaac cgcaatcacg
     7261 gtaataacaa tcgctaaaaa tagagggatg atatgcgagc gtgaatccat tatttccact
     7321 ctttcacttt acaaattaaa tcgtacgccg cttgaatttg ttgagctttc tcttttgcca
     7381 actccatcat ctcatcaggc aaacctttag cggctaattt atccgggtga tgttcgttca
     7441 ttaacttacg ataagcccgt ttcactgtat tttgatcgtc ttgtgcggtt acgcctaata
     7501 cggtataggc tgcctcaatg cttggttgtg caggctgctg ataactgccc tgatagccac
     7561 cttgagattg ctgatgataa ctcccatttt gctgataata gccaccattg cggaattgtt
     7621 gagctgccat aatcattgcg atcatctgct caaattggaa gcgagacatt cccattgttt
     7681 cggcaatcgt aaacagaatt tgttgctcgt tcacatcaag ttgcccatct tgcaaggctg
     7741 cctgcatttg cacttcgaca aagaaacgta ataagtcagt tcttgcccca caggcttctc
     7801 tgaactcttg aatgacttgg cgtaacggga agtcaggctc tttacctaag gtaaaggcat
     7861 tttgcgctaa ttgctgggct gcactatcta attttaagcg agccattaac gctcgggcta
     7921 attcaatatc cgcttcagta acacgccctt ttgctttcgc aatatgcccc aacaccgcaa
     7981 aagtggtttg ggtgaaaagc gattggcgag ttaaattttt accgaaaaga cttgaacgaa
     8041 ctgaacctaa ttcatagagt tttttatcgg ctaaatgccc gataaaagcg ccaagtatcc
     8101 caccaaataa accgtgaaaa agctgatagc ccaagaaaaa accgactatt t
//
LOCUS       DANJWC010000113         8067 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.113, whole genome shotgun sequence.
ACCESSION   DANJWC010000113 DANJWC010000000
VERSION     DANJWC010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8067
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.113"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..155)
                     /locus_tag="PWX02_001768"
     CDS             complement(<1..155)
                     /locus_tag="PWX02_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729762.1"
                     /translation="MDIIINFAIYLQFFIGLFAIVNPFGSLPIFFSMTAHQYEGERNR
                     TSLITAVS"
     gene            complement(277..2148)
                     /locus_tag="PWX02_001769"
     CDS             complement(277..2148)
                     /locus_tag="PWX02_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HDL1729763.1"
                     /translation="MIEKMHERTNGPVFKIIFALVSISFVIGGIGTGLISQDNSVAKV
                     NGEEISQQLFNNTLNREQNRLNVELGSRFWDLMDSPEYAAQFNQSVLNGLIDEELLRQ
                     YAKELKLGISADQIKSEIVNSQMFQQDGKFSNELYQQTLRHNNLSADGYAAIVQEGML
                     QSQLQEGIINSDFNVPAQQALLAKLLLQQREVRLAEFSVAKEMENQTASEQELRAYYE
                     ANKTKLLAPEKLAVEYVTLSPKDVESKVEITNEQIQTYYDRNKADYVTKGEAHLAHIQ
                     VASEAEAQAIEQALKNGEDFATLAKNKSSDTLSANNGGDLGWAKAGTFPKAFEDAVAN
                     LTAGQMSAAVKVDNAYHIIKVLERKPEQVIELAQVKDKIAQTIRQELVLTEYSTVARE
                     MANKAFENNSSLEEVAKAGNVELQKTDLFTRENVPTALQNEKVLKVLFEGELRQNKQN
                     SDALDVGNDANPQTLFVRVSDYDAERPQTFDEAKAAVENFVKAEKAEKALLAKVEENV
                     KALNEGKSVDVKFNAPQTLVYMQAEVENPVLAGTIFAMKKAQDKTTYQMARNQKGDVV
                     IVALDNITEGNAEQFKTLAPQFEQANRLILRNEFLQSLRAKASIEVNEDFMQQRSSH"
     gene            2330..2599
                     /gene="rpsO"
                     /locus_tag="PWX02_001770"
     CDS             2330..2599
                     /gene="rpsO"
                     /locus_tag="PWX02_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDL1729764.1"
                     /translation="MSLSVEAKAKIVAEFGRDAKDTGSSEVQIALLTAQINHLQAHFA
                     EHKKDHHGRRGLLRMVSRRRKLLDYLKRTDLAKYSETIARLGLRR"
     gene            2671..3540
                     /locus_tag="PWX02_001771"
     CDS             2671..3540
                     /locus_tag="PWX02_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HDL1729765.1"
                     /translation="MAFASLFTLLDDIAAVLDDVSIMTKVAAKKTAGVIGDDLALNAN
                     QVSGKNVVPERELPIVWAVAKGSLINKVILIPLALLLSVFLPQAIIPLLMIGGAYLCF
                     EGVEKLLHRLLHKNNQAHSDETETLSEKDKIKGAIRTDFILSAEIIIIALGVLQDSST
                     LTQILSLSAIGVGITLFVYGLVGLIVKLDDIGLWLMQKKSTFSQAVGKGLLFAMPWFM
                     KGLSVIGTIAMFLVGGGIFTHNLPMIHHFVEQFGISPHFSTLVDFAVGIVVGIIACLI
                     ILPLMKLFSKKAH"
     gene            complement(3630..4895)
                     /locus_tag="PWX02_001772"
     CDS             complement(3630..4895)
                     /locus_tag="PWX02_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HDL1729766.1"
                     /translation="MFRTLFLMLLLLVGLISGPYLAGNQGYVRIETATTVIEMSIVML
                     VVFFVLAMAVVYLLEWIVTKICRMSNGAYNWFSTRKHKKAQQETLEGLMRMSEGNYSK
                     AEKLFSKNAKHADEPVLNLIKAAEAAQQNGDDLAANKYLIKATEIAGPNNVAVELVRT
                     RILLQQGKLPAARTAIDSLLELAPHSDEVLRLAIQIYKESRAYVALDRLLVEIGQRNF
                     LTAEEYAELEYFVDDGLQDERLQEEGQEGLLSWWENQPNRRRKSVYSRVGVIKRLIDS
                     DDHQSAQEIALETLKKFEDEQLQGLFVQLTRLQIDSDSKLLKVLAKRADKANVKYTDD
                     YARALGYIYTRDGQYQKAKSQFELLLAHDECVADDRIMALHVAEQMGDTLMANRIREE
                     NLKQVNMATQSKVNEPLALPQTATESNSL"
     gene            complement(4914..6224)
                     /locus_tag="PWX02_001773"
     CDS             complement(4914..6224)
                     /locus_tag="PWX02_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDL1729767.1"
                     /translation="MSRSKKVTEHLEHSAEKVVEEAQQAVDSAESFAKKEIEEDVVNE
                     AIEKDIQTEMQKAPKEQVVVQKSGGKGLALVALLVALAVGGAGHYMANKKFAEVESQI
                     QAVSAKANQPTTQTMVEMPNFENEKAQIAELVTEYQKALERIKALETAQNGYTQQISS
                     LQLQLQKLNNTSGSDNTFWLLSEADFLLNNAARKVVLDNDIETAKNLLLEADQVLSKA
                     PNTIHIREAIKTDLNTLTALHQIDQNALMQRVANLANKLDDLPILESEQASNSKQDGQ
                     VSDSLADWEKNLEKSAASFLDHFIRVSKRNAADEKAFVAPNQEIYLRENIRLRLQIAI
                     LAVPRQQSELYKKSLQTVGSWVRSYFETSNENVQHFLKELDELAEQTIYVDAPTSLQS
                     LKVIAQQINKTPEKIEKVEIKAEKELEQAEPVKAEQDAMPPSAQ"
     gene            complement(6236..7000)
                     /locus_tag="PWX02_001774"
     CDS             complement(6236..7000)
                     /locus_tag="PWX02_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDL1729768.1"
                     /translation="MNVLVTRPDSRGQELVDMLNEQQIFAIHQPLFTIEAGKELPLLP
                     SAISRLKSDDYVFVVSPNAVDYAAKTLVDTGFHFRSDLKYFAVGQRTAKYLTEKAEQA
                     VIYPIEFENSEGVLALPEMQNLTDKTILILRADSGRELLAETAVLRGATIQYLECYRR
                     EPVTDDIPEKISLCKRLGVDTIVITSSEILRSLYEQAKADCRAWLFECDLVVVSRRIA
                     KIAKQMGWQEDKIRVSDKADNVTLMNTLLFKVGKSN"
     gene            complement(7000..7935)
                     /gene="hemC"
                     /locus_tag="PWX02_001775"
     CDS             complement(7000..7935)
                     /gene="hemC"
                     /locus_tag="PWX02_001775"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDL1729769.1"
                     /translation="MKDILRIATRQSPLALWQANFVKSELEKHFPQLSVELVTMVTKG
                     DIILDTPLAKIGGKGLFVKELELALLENRADIAVHSMKDVPMSFPEGLGLAVICERED
                     PRDAFVSNNYHSLEDLPKGAVVGTSSLRRQCQLMAKYPHLEVKSLRGNVGTRLSKLDN
                     GEYDAIILASAGLIRLGLQERIRSYISVEQSLPAAGQGAVGIETRANDERILNYIAKL
                     NHSSTACCVMAERAMNTRLQGGCQVPIGGYATLEGDELTLNALVGSLDGSQIIRASAK
                     GHKQEAEQLGILVAEQLLAQGADKILAAVYNENVQ"
BASE COUNT         2281 a         1752 c         1350 g         2684 t
ORIGIN      
        1 gaaacagcgg tgattaagct ggtgcgattt ctttccccct catattggtg agcggtcata
       61 ctgaaaaaaa taggcaagga accaaaagga ttgactatag caaatagacc aatgaagaat
      121 tggaggtaga tggcaaaatt aataatgata tccacaaaaa ctttctcaaa caacagtgtt
      181 tcgggcgcta ttatatcggt taatcaaggt agatacaaac caaaagcacc cagcctaaag
      241 gacggttggg tgctttttga taaaatgaaa ggcttgttag tgtgacgaac gttgctgcat
      301 aaaatcttca tttacctcaa ttgaagcttt tgcacgtagc gattgtaaga attcattacg
      361 taaaattaaa cgattagctt gttcaaattg tggcgccaat gtcttaaact gttcagcatt
      421 cccttccgta atgttatcta aggcaacaat aactacatcg cccttttgat tacgtgccat
      481 ttgataagtc gttttatctt gcgccttttt cattgcgaaa atcgttccag ccaataccgg
      541 gttttctact tctgcttgca tataaactaa agtttgtggc gcattaaatt tcacgtccac
      601 tgatttccct tcattcaagg cttttacatt ttcttctact ttcgccagca acgctttttc
      661 cgctttttct gccttcacaa aattttcaac tgctgctttt gcttcatcaa aagtttgtgg
      721 acgttctgca tcataatcac tcactctcac aaacaatgtc tgtggatttg catcattccc
      781 aacatctaaa gcatcagaat tttgtttatt ttggcgtagc tctccttcaa acaaaacttt
      841 taatactttc tcattttgta aagcagtagg tacgttttca cgggtaaaca gatccgtttt
      901 ttgcagttcc acattaccgg ctttcgcaac ttcttcaagt gaagaattat tctcaaaggc
      961 tttatttgcc atttcgcgcg caactgtaga atactcagtt aacactaatt cttggcgaat
     1021 agtttgggca attttatctt tcacttgtgc taactcaatc acttgctcag gtttacgctc
     1081 caatactttg atgatatggt aagcattatc cactttcacc gctgcactca tctggccggc
     1141 agttaaattt gctacagcat cttcaaaagc tttcgggaaa gtgccagctt ttgcccaacc
     1201 tagatcacca ccattatttg ctgataaagt atcgctagat ttgttttttg ccaaagtagc
     1261 aaaatcttcc ccatttttga gcgcttgctc aatagcctgt gcttccgctt cactagcgac
     1321 ttgaatatga gctaaatggg cttcaccttt agttacataa tcggctttat tgcgatcata
     1381 gtaagtttga atttgctcat tggtaatctc aactttgctt tccacatctt ttggagaaag
     1441 agtaacgtac tcaaccgcta atttttcagg tgctaataat ttagttttat ttgcttcgta
     1501 ataggctcgc aattcttgct cggaagcggt ctgattttcc atttcttttg caactgaaaa
     1561 ttcggctaaa cgcacttcac gttgttgcaa cagtaatttt gctaacaatg cctgctgtgc
     1621 cggtacatta aaatcactat tgataatccc ctcttgtagc tgggattgca acataccttc
     1681 ctgcacaatc gctgcatagc catcggcact taaattattg tggcgtaatg tttgttggta
     1741 taactcgtta ctgaatttac catcttgttg aaacatctgg ctgtttacaa tttcagattt
     1801 gatttgatca gcactaatac ctagttttaa ctcttttgca tactggcgca gaagctcttc
     1861 atcaattaat ccatttaata ccgattgatt aaattgagca gcatactccg ggctatccat
     1921 taaatcccaa aagcgactcc ccagttcaac atttaatcta ttttgctcac gattaagcgt
     1981 gttattaaat aattgctgac taatttcttc cccatttact ttcgctacag aattatcttg
     2041 tgaaatcaat cctgtcccaa ttccaccgat tacaaatgaa atagagacta atgcaaaaat
     2101 aattttaaag accggcccat ttgtccgctc gtgcattttt tcaatcattt aaaaattcct
     2161 aaattactca aaaattgtcg ctgattatac tctttctcaa atgagaaata aacttgcaaa
     2221 tttttattga aatttaaccg ctttctcttt aaaattcaca gtccctgtcg tagctgagtt
     2281 agagatcggc tattgatgta ttatcaaccc ttaacaaaag gaaattaaaa tgtctctaag
     2341 cgtagaagca aaagcaaaaa tcgttgctga atttggtcgt gatgcaaaag acactggttc
     2401 ttctgaagtt caaattgcat tattaactgc acaaatcaac cacttacaag cacactttgc
     2461 tgagcataaa aaagatcacc acggtcgtcg cggtttatta cgtatggttt cacgccgtcg
     2521 taaattatta gactacttaa aacgtactga tcttgctaaa tattcggaaa ctatcgcacg
     2581 tttaggttta cgtcgctaat ttcaaatatt agatatgaga gccgagctga aaagttcggc
     2641 ttttttataa attttattcg aggaaaaaat atggcttttg cgtcattatt tacattatta
     2701 gacgatattg ctgccgtgct tgatgatgta tcgatcatga ctaaagtggc tgctaagaaa
     2761 acagcaggcg taatcggtga tgatcttgcc ttaaatgcta atcaagtcag tggaaagaat
     2821 gtggtgccag aacgagaact accgattgtt tgggctgtag cgaaaggctc attaatcaat
     2881 aaagtgatct tgattccact agcgttgctg ctatcggtct ttttaccgca ggctattatc
     2941 ccgctattaa tgataggcgg tgcatattta tgttttgaag gcgtagagaa attactccat
     3001 cgtctattac ataaaaacaa tcaagctcat tctgatgaaa ccgaaaccct ttctgaaaaa
     3061 gataaaatta aaggtgcaat tcgcaccgat tttattctat cagcagagat tatcattatt
     3121 gcgttaggtg ttttgcaaga ctcttcaacc ctaacccaaa ttttatctct ctccgctatc
     3181 ggggtcggaa ttaccctgtt tgtttatggc ttagttggat taattgtaaa actagatgat
     3241 atcggtttat ggttaatgca gaaaaaaagc acattctcac aagcggttgg aaaaggctta
     3301 ttatttgcaa tgccgtggtt tatgaaagga ctttctgtga ttggtactat tgcgatgttc
     3361 ttagtcggtg gtggtatttt tacccataat ttaccaatga ttcatcactt cgtagaacaa
     3421 ttcggtatct ctccccattt ttctactctg gttgattttg cagtgggtat tgtggttggt
     3481 attattgcat gcttaattat tttacctttg atgaaattat tttctaaaaa ggcgcactaa
     3541 tccccaaaag cgtagctttg gttaaagcta gctttttatc tcttgctata aaaaaatagc
     3601 aggtttcaag cctgctattt ttttaattat tataatgagt tagactcagt ggctgtttgc
     3661 ggtaaggcta atggctcatt aactttagac tgagtagcca tattcacctg tttgagattt
     3721 tcctcacgaa tacggtttgc cattaatgta tcccccatct gctctgccac gtgcagtgcc
     3781 ataatacgat catcagcaac acattcatca tgagccaata acaattcaaa ctgagacttc
     3841 gctttttggt attgcccatc tcgtgtataa atatagccaa gcgctctagc ataatcatcg
     3901 gtatatttaa cattcgcttt atctgcccgt tttgctaata cttttagtag cttactgtca
     3961 gaatcaattt gtaaacgggt taattgcaca aataaacctt gtaattgctc atcttcaaat
     4021 ttcttcaacg tttctaaagc aatttcttgt gcagattgat gatcgtcact atcaattaaa
     4081 cgcttgatga caccgacacg actatacacc gatttacgac gacgatttgg ttggttttcc
     4141 caccaagaaa gcaagccttc ctgcccttct tcctgtaaac gctcatcttg taaaccatca
     4201 tcaacgaaat actctaactc agcatactct tctgcagtta agaaattacg ttggccgatt
     4261 tcaactaata atctatctaa cgcaacataa gctctggatt ctttataaat ttgaatcgct
     4321 aagcgtaata cctcatcgct atgaggtgca agctctagta ggctatcaat cgcagttcgg
     4381 gcagccggta attttccttg ttgtaataaa attcgggttc gtaccaactc cactgcaaca
     4441 ttattcggtc cagctatttc agttgcctta attaaatatt tatttgcagc taaatcatca
     4501 ccattttgct gagcagcttc cgccgcttta attaaattca gtacaggctc atctgcatgt
     4561 tttgcatttt tactaaagag tttttccgct tttgagtaat tcccttcact catacgcatt
     4621 aaaccttcca gtgtttcttg ctgagctttt ttatgcttac gagttgagaa ccagttataa
     4681 gctccattac tcatacggca tattttagtg acaatccatt caagtaaata aactaccgcc
     4741 attgccaaca caaaaaaaac cacaagcatt acaatgctca tttcaatcac ggtagttgct
     4801 gtttcaattc tgacataacc ttgatttccg gctaaataag gcccagagat tagtccaact
     4861 aataataaaa gcatcaaaaa gagcgttcta aacataatct attctccttt ttattattgt
     4921 gctgaaggag gcatcgcatc ttgctctgct tttacaggct ctgcttgctc taattctttt
     4981 tcagctttta tttcgacttt ctctattttt tcaggggttt tattaatttg ctgagcaatc
     5041 acctttaagc tttgtaatga agttggagca tccacataaa tagtttgttc tgccaactca
     5101 tctagctctt tcaagaaatg ttgcacattt tcatttgaag tttcaaaata acttcttacc
     5161 caagagccta ccgtttgtaa cgatttttta taaagttcgc tttgctggcg aggaacagct
     5221 aaaatcgcaa tttgtaaacg caagcgaatg ttttcacgca aataaatttc ttgattcggt
     5281 gcaacaaagg ctttctcatc tgctgcatta cgtttcgata cacgaataaa atgatctaaa
     5341 aatgaagcag cacttttttc taaatttttc tcccaatccg caagagaatc acttacttgc
     5401 ccatcttgtt tgctatttga agcttgttca ctttccaaaa tcggcaaatc atctaattta
     5461 ttcgctaaat ttgcgacacg ctgcattaag gcattttgat cgatctgatg caatgcggtt
     5521 aatgtattta aatcagtttt gattgcctca cgaatgtgaa tcgtattcgg tgcttttgat
     5581 aaaacttgat ctgcctctaa caataaattt ttcgctgttt caatgtcatt atctaacacc
     5641 actttacgag ccgcattatt caataggaaa tctgcctcag ataatagcca gaatgtgtta
     5701 tccgaaccag aggtattatt tagcttttgc agttgaagct gcaaactact aatttgctgg
     5761 gtataaccat tttgggctgt ttctaaagct ttaatccgtt ctaaagcttt ttgatattcc
     5821 gtgaccaatt cggcaatttg tgctttttca ttttcgaaat ttggcatctc aaccatagtt
     5881 tgagtagtag gctgatttgc tttagcagat actgcctgaa tttgagattc aacctctgca
     5941 aactttttat ttgccatata atgaccagct ccgccaaccg ctaaagccac taatagtgca
     6001 actaatgcca agccctttcc gcccgatttt tgaacaacca cttgttcttt tggcgctttt
     6061 tgcatttctg tttgaatatc tttctctata gcttcgttca ctacatcttc ctcaatctct
     6121 ttttttgcaa aagattccgc tgaatcgacc gcttgttgtg cctcttctac caccttttca
     6181 gctgaatgct ctaaatgttc agttactttt ttagatcttg acatactcgc cacctctagt
     6241 tagatttgcc cactttaaat aataatgtat tcatcaaagt tacattatcg gctttatccg
     6301 ataccctaat cttatcttct tgccagccca tttgtttagc tatctttgca atccgccggc
     6361 tcactacaac cagatcacat tcaaataacc atgctctaca atctgctttc gcttgttcat
     6421 aaagtgatct taaaatttca ctactggtga tcacaatagt atctactccc aagcgtttac
     6481 ataagctgat tttttcggga atatcatcgg taaccggctc tctacgataa cactccaaat
     6541 attgaatagt tgccccacgc aagactgcag tttcagcaag tagctcacgc ccagaatcgg
     6601 ctcttagaat taaaatagtt ttatcagtta gattctgcat ttcaggtaat gccaaaaccc
     6661 cctcgctatt ttcaaattct atcggataaa tgaccgcttg ttcagctttt tctgttaaat
     6721 atttcgcagt ccgttgccct accgcaaaat atttcaaatc tgaacgaaaa tggaacccgg
     6781 tatctactaa tgtttttgca gcatagtcca ctgcattcgg cgaaacaaca aatacataat
     6841 cgtctgattt tagccgagaa atagccgaag gaagcaaagg taattcttta ccggcttcaa
     6901 tcgtaaatag cggttgatga atagcaaaaa tttgctgttc attcaacata tctaccaatt
     6961 cttgcccccg actatccggg cgtgtgacca acacattcat tattgtacgt tttcgttata
     7021 aaccgcagct aaaattttat ctgcaccttg agcaagcaac tgttccgcca ccaaaatgcc
     7081 taattgctcc gcctcttgct tatgtccttt tgccgaagcc cgaataattt gtgagccatc
     7141 taacgatcca actaacgcat taagcgttaa ttcatcgccc tctaatgtag cataaccacc
     7201 aatcggcact tggcaaccgc cttgcaaacg ggtattcatt gcacgttctg ccatcacgca
     7261 acaagcggtc gaactatggt ttaattttgc aatgtagttc aaaatccgtt catcattggc
     7321 tcgagtctca atccctactg ctccttgacc agctgcgggt aatgattgtt ccacagaaat
     7381 atagctacgg attcgctctt gcaagcctaa acgaattaag ccggcagagg ctaaaataat
     7441 cgcatcatac tcgccattat ctaatttgct taagcgagtg ccgacattgc ctcgcaatga
     7501 tttaacctct aaatgcggat atttcgccat taattggcac tgacggcgta agctcgatgt
     7561 accgaccaca gcacctttcg gcaaatcttc taacgaatgg tagttgttcg acacaaacgc
     7621 atcacgggga tcttcacgtt cacaaatcac cgcaagcccc aagccttcgg gaaagctcat
     7681 cggcacatct ttcattgaat gcaccgcaat atcagctcga ttttctaaaa gggctaattc
     7741 taactctttc acaaacaagc cttttccgcc aattttggct aatggggtgt ctaaaataat
     7801 atcgcctttc gttaccatcg taaccagttc gacagataat tgcggaaaat gtttttctaa
     7861 ttcgcttttt acaaaatttg cctgccataa ggctaacggg ctttgacggg ttgcaatgcg
     7921 taaaatatct ttcataaatt ctaaaattaa aacattgagt taatgaatta tcctaccact
     7981 ttttagctga actgccaaaa ataatcgtgg atttcgcttt gcatttaatc aaaaatccgg
     8041 ctttttctca aaatgtatca atatcta
//
LOCUS       DANJWC010000114         8054 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.114, whole genome shotgun sequence.
ACCESSION   DANJWC010000114 DANJWC010000000
VERSION     DANJWC010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8054
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.114"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            186..980
                     /locus_tag="PWX02_001776"
     CDS             186..980
                     /locus_tag="PWX02_001776"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDL1729770.1"
                     /translation="MRLIPLETDHDVSVWAARYIVDRINAFQPTKEKPFVLGLPTGGT
                     PEKTYKELIKLYQAGEVSFKNVVTFNMDEYVGLPKEHPESYHSFMFKHFFDHVDIPLE
                     NINILNGMAEDVNAECERYEEKIRFYGKIHLFMGGVGVDGHIAFNEPASSLSSRTRIK
                     TLTEDTRIANSRFFNNDVSQVPKFALTVGVGTLLDAEEVMILVTGHNKALALQACVEG
                     AVNHLWTISCLQLHRRGIVVCDEPATQELKVKTVKYFKELEKNVAR"
     gene            1061..2203
                     /gene="nagA"
                     /locus_tag="PWX02_001777"
     CDS             1061..2203
                     /gene="nagA"
                     /locus_tag="PWX02_001777"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDL1729771.1"
                     /translation="MNHYALTNCVIYTGEQVLYQHALVVKEDKIDSIVLEENLPLDLQ
                     KIDLKGANLSAGFIDLQLNGCGGVMFNEEITTRTLEIMQETNLRSGTTSYLPTFITSP
                     DEGVKQAVAVMREYLSKHKNQALGLHLEGPYLSVAKKGVHRPEFIREISPEMLDFLCD
                     NGDVITKITIAAENPTADHIKRFNDAGIIVSIGHSNATFAQTKQRIAEGAAFATHLHN
                     AMSPVSNGREGGVVGAVLDSDIYTGIICDGLHVEWGNIRIAKKAKGDKLVIVSDAVAA
                     AGSDIESFIFVGKKVTVKDGKCYDEFGALGGAAITMIESIRNVVQHCDISLNEAIRMS
                     TLYPAKAIGVEETLGSLEAGKIANLAIFDNQFEVKATAVNGQLQWH"
     gene            2285..2599
                     /locus_tag="PWX02_001778"
     CDS             2285..2599
                     /locus_tag="PWX02_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HDL1729772.1"
                     /translation="MQFKDYLATFPTIDHLAGLNVINSGEVIQHIPAIEGKLGSLKLY
                     NALFAKFDGKLTASAAKQGLEWFAEHVEDAKNNVGKHPNIDLLFRVIEQDLSLSLEPI
                     VK"
     gene            complement(2678..3868)
                     /locus_tag="PWX02_001779"
     CDS             complement(2678..3868)
                     /locus_tag="PWX02_001779"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDL1729773.1"
                     /translation="MTQAIESVLAELKRGVENIYSEEDLITKLKENRPLRIKLGADPT
                     APDIHLGHTVVLNKLRQFQQFGHEVYFLIGDFTGMVGDPSGKNSTRPPLSREDVLRNA
                     ETYKEQIFKILDPQKTKIVFNSEWLGKLGTEGMIRLASNYTVARMLEREDFKNRFTNQ
                     QPIAIHEFIYPLLQGHDSVELKADVELGGTDQTFNLLVGRELQKSAGQKPQVAMTLPL
                     LVGLDGEKKMSKSLGNYIGVTEAPSEMFGKIMSISDDLMWDWYNLLSFRPLDEIAQLK
                     ADVENGKNPRDVKILLAKEIIARFHDEAAADAAEQEFVNRFQKGAIPDEVPEFTFEGE
                     IGLAALLKEAGLVPSTSEAIRSAQQGGVKIDGEKVEDVKQTAQKGTFVYQVGKRKFAR
                     ITVK"
     gene            complement(4231..5535)
                     /locus_tag="PWX02_001780"
     CDS             complement(4231..5535)
                     /locus_tag="PWX02_001780"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HDL1729774.1"
                     /translation="MESIIALFKGMGLMHLELGQAIMIVISLVLLWLAIARKFEPLLL
                     LPIGFGGLMSNIPEAGMAMTALDNLLHSGSAEQLAIIAAKVNSTSLDLHSIKDAIALA
                     APSVQNELEVMAGDMGYTAGVLALFYKVAIGYGVAPLIIFMGVGAMTDFGPLLANPRM
                     LMLGAAAQFGIFATVLGALGLNWLGIIDFTLPQAAAIGIIGGADGPTAIYLASKLAPE
                     LLGAIAVAAYSYMALVPLIQPPIMKALTTEQERKIRMVQLRNVSNREKIIFPIVLLLL
                     VALLLPDAAPLLGMFCFGNLLRTSGVVERLNDTAQNALINIVTIFLGLSVGAKLVADK
                     FLQPQTLGILVLGVIAFGIGTASGVIMAKIMNRFSQHKINPLIGSAGVSAVPMAARVS
                     NKVGQEYDKQNFLLMHAMGPNVAGVIGSAIAAGVMLKYISTM"
     gene            complement(5545..7371)
                     /gene="oadA"
                     /locus_tag="PWX02_001781"
     CDS             complement(5545..7371)
                     /gene="oadA"
                     /locus_tag="PWX02_001781"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HDL1729775.1"
                     /translation="MTTQPKKIAITDVVLRDAHQSLFATRLRLDDMLPIASELDNIGY
                     WSLETWGGATFDSCIRFLGEDPWVRLRELKKAMPKTPMQMLLRGQNLLGYRHYADDVV
                     DKFVERSVANGMSVFRVFDALNDPRNMQAALQAVKKHGGHAQGTLSYTTSPVHTLQTW
                     LDVTEQLLEIGVDSLVIKDMSGILTPMAAYELVSEIKKRYDVELHLHCHSTTGMAEMA
                     LLKAIEAGVDGVDTSISSMSGTYGHPATESIVATLQGTPYETGLSIPALEKISAYFRN
                     VRKKYAKFEGQLRGIDSRILVAQVPGGMLTNLESQLKQQNASDKLDLVLQEIPRVRED
                     LGNIPLVTPTSQIVGTQAVMNVLMGERYKTIAKETAGILKGEYGRTPAPVNKALQDRV
                     LEGAEPITDRPADHLPPELEKLVAEVTASAKEKGITLSENMIDDVLIVALFQQVGWKY
                     LENRNNPSAFEPVPSADEAKPAQASAASAPKAQTQSGSAIYTVELEGKAFVVKVSEGG
                     DITNIAPTPATPAPQAVDSAPKSANTLSANAEPVNAPMAGNILKVEVKEGQQVAEGDV
                     LLILEAMKMETQICAAKSGTIQGINVKQGDVVAVNDTLMSIA"
     gene            complement(7411..7665)
                     /locus_tag="PWX02_001782"
     CDS             complement(7411..7665)
                     /locus_tag="PWX02_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit gamma"
                     /protein_id="HDL1729776.1"
                     /translation="MAPAELFAEGINLMVAGMGFVMLFLLVLIYAISFISKIINQYFP
                     EPVSIPASKTVPQSPPDDLERLRPVIAAAIAHHRRINGQH"
     gene            complement(7855..>8054)
                     /locus_tag="PWX02_001783"
     CDS             complement(7855..>8054)
                     /locus_tag="PWX02_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729777.1"
                     /translation="VFVGTLNGLILPISLGLILLAAHKTKIVGDYKHPVWMTVSGAIV
                     VILMAVLSAMTLSKYISGLMA"
BASE COUNT         2287 a         1691 c         1774 g         2302 t
ORIGIN      
        1 agtagcaaaa gtgatttttt cgatctcgat cccaaaaatg atttttgggt attgattttt
       61 caatcgataa attttctgtg atcaggatca caaaaatagc agaaaaaata gaaattgaag
      121 ttgtatttca ttaaaataat aaaaaatgaa gtgatttatt cagaattgat atttaaggag
      181 agactatgcg tttaattccg ttagaaactg atcacgatgt gagtgtttgg gctgctcgtt
      241 atattgttga tcgtatcaat gcattccaac caaccaaaga aaaacctttt gtgcttggct
      301 taccaaccgg cggtacgcct gaaaaaacct ataaagaatt aattaaatta tatcaagctg
      361 gtgaagtgag ctttaaaaat gtggtgactt tcaatatgga cgaatacgtc ggcttaccta
      421 aggaacaccc tgaaagctac cacagtttta tgttcaagca cttttttgac cacgttgata
      481 ttccgcttga gaatatcaat atcctcaacg gtatggcgga agacgtgaat gcagaatgcg
      541 aacgttatga agaaaaaatc cgtttttatg gtaaaatcca tctgtttatg ggcggggtcg
      601 gtgtggatgg gcatattgcg tttaatgagc ctgcatcttc gctttcttcc cgcactcgaa
      661 ttaaaacgct taccgaagat actcgcattg caaactcacg tttctttaac aatgacgtaa
      721 gccaagtgcc gaaatttgct ttaaccgttg gggttggtac gttgcttgat gccgaagaag
      781 tgatgatctt agtgaccggc cataacaaag ccttagcatt acaggcgtgt gtggaagggg
      841 cggtaaatca tctttggacc atcagttgct tacaattaca tcgccgtggt atagtggtgt
      901 gtgatgagcc ggcaacccaa gagctgaaag ttaaaacagt taagtatttc aaagagttag
      961 agaaaaacgt agctcgctaa ttctgatttt acaagcggtt aattttgtaa tattttttac
     1021 aaaaacgacc gcctgttagc atttaaagag aagagagaaa atgaaccatt atgccttaac
     1081 aaactgtgtg atttacaccg gtgaacaagt gctttaccaa cacgcattag tggtgaaaga
     1141 agataaaatc gactccattg ttttggaaga aaatttaccg ctggatctgc aaaaaatcga
     1201 cctaaaagga gcgaatcttt cagccggttt tattgacttg caactgaacg gctgcggtgg
     1261 ggtgatgttt aatgaagaaa tcactacccg cactttagaa attatgcagg aaaccaatct
     1321 gcgttcaggc acaaccagct atttaccgac ttttattacc tcgcctgatg agggagtgaa
     1381 acaagcggtg gcggtaatgc gtgagtattt gtcgaaacac aaaaaccaag cactcggctt
     1441 gcatttggaa ggaccttatc taagtgtggc aaagaaagga gtacaccgtc cggaatttat
     1501 ccgtgaaatc agccctgaaa tgttagattt cttatgcgat aacggcgatg taattacgaa
     1561 aatcacgatt gcagcagaaa acccgaccgc agaccatatc aaacgcttta atgatgcagg
     1621 aattattgta tctatcgggc attccaatgc cacttttgct caaaccaaac agcgaattgc
     1681 cgaaggggca gcgtttgcta cgcatttaca caatgcaatg tcgccggtca gcaacgggcg
     1741 tgaaggcggt gtagtagggg cggtgttgga tagcgatatt tacaccggca ttatttgcga
     1801 tggcttgcac gttgaatggg gcaacattcg cattgccaaa aaagccaaag gcgataaatt
     1861 agtgattgtc agcgatgctg ttgctgccgc aggaagtgat attgagtcgt ttatttttgt
     1921 cggtaaaaaa gtcacggtga aagatggcaa atgctacgat gaattcggag cattgggcgg
     1981 tgcggcaatc acgatgatcg aatccattcg caacgtagtg caacattgcg atattagcct
     2041 gaatgaagca atccgaatga gtacccttta cccggcaaaa gcgattgggg tagaggaaac
     2101 actcggctca cttgaagccg gcaaaattgc caaccttgcg attttcgata atcaatttga
     2161 agtaaaagcg actgctgtaa acgggcaatt acagtggcat taattttgct acaaattcta
     2221 agcataacag catataatcc aaaggcaggc attgtgtctg ccttttattt atagggagaa
     2281 taacatgcaa tttaaagatt acttagccac ttttccaact attgatcatc tagccggctt
     2341 aaacgtgata aatagcggtg aagtgattca acatattcct gcgattgaag gtaaattagg
     2401 ttcactaaaa ctttacaatg cactgtttgc gaaatttgat gggaaattaa ccgctagtgc
     2461 agcaaaacaa gggttggaat ggtttgccga acacgttgaa gatgcgaaaa ataatgtggg
     2521 taagcacccg aatattgatc tattatttag ggtaattgag caagatttat ctttaagttt
     2581 agagccgatt gttaaataag attgcactag tgaaaaataa caagcggtca ttatctgcaa
     2641 aatttttgca aataatgact gcttgtgttt aagaggctta tttcacggta atgcgtgcaa
     2701 atttgcgttt gccgacttgg taaacaaaag tgcctttttg agcagtttgt ttcacatctt
     2761 ccactttttc accatcaatt ttaaccccgc cttgttgggc tgagcgaatt gcctctgaag
     2821 tggacggtac taagccggct tctttcagta gagccgctag gccgatttca ccctcaaagg
     2881 taaattcagg cacttcatca ggaattgccc ctttttggaa acggttgaca aactcttgct
     2941 ctgctgcatc tgctgccgct tcatcgtgga aacgagcgat aatttctttt gccagtagaa
     3001 ttttcacatc acgcgggttt ttgccatttt caacatccgc ttttaattgt gcaatttcgt
     3061 ctaatggacg gaatgagagc aggttgtacc aatcccacat taaatcgtct gaaatcgaca
     3121 taattttgcc gaacatttcg cttggagctt cagttacacc aatgtagttg ccgagagatt
     3181 tcgacatttt tttctcgccg tctaagccga caagtagtgg gagtgtcatt gccacttgtg
     3241 gtttttgacc ggctgatttt tgtaactcac gtccaactaa caagttaaag gtttggtctg
     3301 taccacctaa ttccacgtca gctttcagtt ctaccgagtc gtgaccttgt agtagcggat
     3361 aaataaattc gtggattgcg atcggttgtt ggttcgtgaa acggtttttg aaatcttcac
     3421 gttctaacat tcgagcaacg gtgtagttgg aggctaagcg gatcatacct tccgtaccta
     3481 acttgcctaa ccattcggag ttgaatacga tcttggtttt ttgtggatct aaaattttga
     3541 agatctgctc tttataggtt tcggcattgc gtaacacgtc ttcacggcta agtggcggac
     3601 gggtagagtt ttttcctgat ggatcgccta ccataccggt gaaatcaccg attaagaaat
     3661 agacttcgtg cccgaattgt tggaattgac gaagtttgtt cagcacaaca gtatgaccta
     3721 agtgaatatc cggtgcagtt gggtctgccc ctaatttaat acgcaatggg cggttttctt
     3781 ttaatttggt gattaaatct tcttctgaat aaatattttc cacgccacgt ttaagttcag
     3841 caaggactga ttcgatagct tgagtcattt atcttccttt tagtcttgaa tataaaaaat
     3901 ggatctatta tagcggtatg aattgaaatt gataggctat aggcgaaaaa aaacgccgta
     3961 ttttgtgggg gaatacggcg taaagagttg gagtgattac ctattatttt tgaagtgatt
     4021 taatgataat gattatcatc tgttaagtca atactttttt tgaaaatttt caataagaat
     4081 tattctcatc aattaagtaa gatggatact acggcaatta gtgaagaatg tacagcggaa
     4141 ttttcgtatt gtaagagtgt tggtaagcgg tatttttaga aaaaacctcc gagagcggtt
     4201 atttttcgga ggttttttgc aaatgaggat ttacatcgtt gaaatgtatt tcaacatcac
     4261 ccccgctgca atcgcagagc caatcacgcc ggcaacgttt gggcccattg cgtgcattaa
     4321 caagaagttt tgtttatcgt actcttgtcc gactttgttt gatacccgtg cagccatcgg
     4381 cacggcagaa acacccgctg agccgataag tgggttgatt ttgtgctgac taaagcggtt
     4441 cattattttc gccataatca caccgcttgc agtgccgatc ccaaaggcaa tcacgcccaa
     4501 tactaaaatg cctagtgttt gcggttgtaa gaatttgtct gcgaccaatt ttgcccccac
     4561 cgataaccct aagaaaatcg tcacgatatt gattaacgca ttttgggcgg tgtcgtttaa
     4621 gcgttctact acgccactgg ttcgcagtaa gttgccaaag cagaacattc ccaataacgg
     4681 tgcagcatcc ggcagtaaga gtgccactaa taatagcaac acgataggga aaataatttt
     4741 ctcacggttg cttacattac ggagctgaac cattcggatt ttacgttctt gctcggtggt
     4801 taaggctttc ataatcggcg gctgaatcag tggcactaac gccatataag aataagccgc
     4861 taccgcaatc gcacctaata attcaggggc aagtttgctg gcaagataaa tcgcggtcgg
     4921 gccatctgcc ccgccgataa tcccaatcgc agcggcttgt ggcaatgtga agtcgataat
     4981 gcctaaccag tttagcccta atgcacccag tacggtagca aaaatcccaa attgagccgc
     5041 agccccaagc attaacattc gagggttggc aagtaaaggg ccgaaatcag tcatcgcccc
     5101 gacacccata aagataataa gcggagcaac gccgtagccg atagcaactt tatagaataa
     5161 tgccaacaca ccggcggtat agcccatatc gccagccatt acttcaagct cattttgaac
     5221 agagggagca gccagtgcga tggcatcttt aatcgaatgc agatcaaggc ttgtactatt
     5281 taccttagca gcaataattg ccagttgctc ggcagaaccg ctgtggagta aattgtctaa
     5341 tgccgtcatt gccatacctg cttccggaat gtttgacatc aaaccaccaa agccaatagg
     5401 cagtagtaaa agcggttcga attttcgggc gatggcaagc caaagcaaca ctaaactgat
     5461 gacaatcata atcgcttgcc ccagttctaa atgcattagc cccatacctt taaaaagtgc
     5521 aataatactt tccataccgg actcctaggc aatactcatt aaagtatcat ttaccgccac
     5581 aacatcgcct tgtttgacat taatgccttg aatcgtgccg gattttgccg cacaaatttg
     5641 ggtttccatt ttcattgctt caagaatcag taacacatcg ccttcagcaa cttgttgccc
     5701 ctctttcact tccactttca aaatgttccc tgccattgga gcattcaccg gttcggcatt
     5761 ggctgatagc gtatttgcag attttggtgc agaatcgacc gcttgcggag ccggtgttgc
     5821 tggtgttgga gcgatattgg ttatatcgcc accttcactc accttcacca caaaggcttt
     5881 gccttcaagc tcaacggtat aaatggctga accggattgt gtttgggctt ttggtgcaga
     5941 agcagcggag gcttgtgccg gttttgcttc atcagcactt ggcactggct caaaggcaga
     6001 cggattattg cggttttcca aatatttcca gcccacttgt tggaagagtg ccacaatcaa
     6061 tacatcatca atcatgtttt cagatagcgt aatgcctttt tcttttgcac ttgcagtcac
     6121 ttctgccact aatttttcca gttccggcgg aagatgatcg gctgggcggt cggtaatcgg
     6181 ctctgctccc tcaagcactc tgtcttgcaa ggctttgttt accggtgccg gtgtgcgacc
     6241 atattcgcct tttaaaatac ctgcagtttc tttggcgata gttttataac gttcgcccat
     6301 taatacgttc atcaccgctt gtgtgccgac aatttgtgag gttggtgtca ccaacggaat
     6361 attgcctaaa tcttcacgca cacgaggaat ttcttgtaaa accaagtcta atttatccga
     6421 cgcattttgt tgcttgagtt ggctttcgag gttggtcagc atgccacccg gcacttgggc
     6481 aaccaaaata cggctatcta taccacggag ctgtccttca aattttgcat attttttacg
     6541 cacgttacgg aagtaggcag agattttctc cagagctgga atgctcaagc cggtttcata
     6601 cggcgtgcct tgaagtgtgg cgacaataga ctcggttgcc gggtgaccgt aagtgccgga
     6661 cattgatgaa atggaggtat cgacaccatc gacaccggct tcaatcgctt tgagcaacgc
     6721 catttcagcc ataccggtgg ttgaatggca atggagatgt agctcaacgt cgtagcgttt
     6781 tttaatttcg ctgactaatt cataggctgc cattggggtt aaaatgcccg acatatcttt
     6841 gatcaccaac gaatccacgc caatttccaa taattgctcg gttacatcca accacgtttg
     6901 tagggtatga acagggctgg tggtgtaact caaagtacct tgtgcgtgcc cgccgtgttt
     6961 tttgaccgct tgtaatgcgg cttgcatatt gcgagggtcg ttgagggcat caaatacacg
     7021 gaacacactc ataccgtttg ccaccgaacg ctcgacaaat ttatctacca catcgtcggc
     7081 ataatgacga taacccaata agttttgccc acgtagcagc atttgcattg gggttttcgg
     7141 cattgctttt ttcagctcac gcaaacgcac ccacggatct tcgcctaaaa aacgaataca
     7201 actgtcaaaa gttgccccgc cccaagtttc taacgaccaa tagccgatat tatccagctc
     7261 ggaggcaatc ggtaacatat cgtctaagcg taaacgagtc gcaaaaaggg attggtgtgc
     7321 atcacgcaaa accacatcgg taatcgcaat ttttttcggt tgagttgtca taatcaaatt
     7381 ccttctaatt attttatttt tgaaatcttg ttaatgttgc ccgttaattc gacgatgatg
     7441 agcaatcgca gcggcaatca cagggcgtaa acgctctaaa tcatctgggg gagattgagg
     7501 cacagtttta gaggctggaa tagaaacagg ttcggggaaa tattgattga taatcttgga
     7561 aataaagctg atggcataaa ttaaaaccag caggaacagc ataacaaatc ccattccggc
     7621 taccattaaa tttatacctt ccgcaaagag ttctgcaggt gccataattt atccttaaaa
     7681 tgtagaaatg atgaagaaaa tgtagaaaaa attcagattt atttcaaatt atttagcctc
     7741 aaaatgagtg gtagatcaca aactttaaac gcaagcggtc agattttaca aattttttac
     7801 aagagtagtt aaaatttgca aaacctgacc ttaggttagc ttgaagctaa gtcgttatgc
     7861 cattagcccc gaaatatatt ttgaaagggt cattgcactt agcaccgcca ttagaatcac
     7921 cacaatcgcc cctgaaacgg tcatccacac agggtgtttg tagtcgccca caatcttggt
     7981 tttgtgagct gccagcagaa ttaacccgag ggaaatcggt agaattaagc cgttaagcgt
     8041 ccctacaaac acca
//
LOCUS       DANJWC010000115         8000 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.115, whole genome shotgun sequence.
ACCESSION   DANJWC010000115 DANJWC010000000
VERSION     DANJWC010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8000
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.115"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..986
                     /gene="icd"
                     /locus_tag="PWX02_001784"
     CDS             <1..986
                     /gene="icd"
                     /locus_tag="PWX02_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019750916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDL1729778.1"
                     /translation="LELIRQYHVAIKGPLMTPVGGGIRSLNVAMRQGLDLYNCLRPIR
                     YYDGTPSPVKHPELVDMVIFRENSEDIYAGVEWVAGSPEANKVIEFLQQEMGVKKIRF
                     TEDCGIGIKPVSKQGTQRLVRAALQYVIDNNRKSLTLVHKGNIMKFTEGAFKEWGYQV
                     AEEFGAKLIDKGPWMSLTNPKTGEEIIIKDSIADAFLQEILLHPTEYDVIATLNLNGD
                     YISDALAAQVGGIGISPGANIGFEAAIFEATHGTAPKIAGQNKGNPGSLILSGEMMLR
                     HLGWVEAADLVVKAVSKTIANKTVTFDFAEMLEGVTLRSTSEFAQDIIANM"
     gene            1006..3612
                     /gene="acnB"
                     /locus_tag="PWX02_001785"
     CDS             1006..3612
                     /gene="acnB"
                     /locus_tag="PWX02_001785"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014391308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HDL1729779.1"
                     /translation="MENFIENYQQQVDERAALGVVPQPLSAEQTAQLVELLKNPPAEQ
                     AVFLLDLFTNRIPAGVDEAAYVKAAFLADITKGVATSPLISPAHAVKLLGTMQGGYNI
                     ETLLSALDNPELAPIAVEALSKTLLMFDNFHDVKERAEQGNPFAQQVLESWANADWFT
                     SRPKLAEKLTVTVFKVTGETNTDDLSPAQDAWSRPDIPLHALAMLKNERDGIFPDENG
                     HTGPIKQLEALKEKGFPLAYVGDVVGTGSSRKSATNSVLWFMGEDIPYIPNKRAGGVV
                     LGGKIAPIFFNTLEDAGSLPIEVDVTNLNMGDVIDIYPYASKICKHNSDEVLAEFELK
                     TEVLLDEVRAGGRIPLIIGRGLTHKARVALGLPESDVFIKPQSVSESNKGYTLAQKMV
                     GRACGVEGVRPGQYCEPRMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAA
                     YPKPVDVITHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIG
                     ISFPAGSGLVAFAAATGVMPLDMPESVLVRFSGTMQPGITLRDLVHAIPYYAIQQDLL
                     TVEKKGKKNIFSGRILEIEGLEDLKIEQAFELSDASAERSAAACTIKLNKEPIIEYLN
                     SNITLLKWMIAEGYDDKRTLERRIANMQAWLDNPELLEADENAEYAAVIEINLDEIKE
                     PIVCVPNDPDDARLLSQVQGDKIDEVFIGSCMTNIGHFRAAGKLLSQAQGELPTKLWI
                     APPTKMDAGLLTEEGYYSIYGKSGARVEMPGCSLCMGNQARVANNANVVSTSTRNFPN
                     RLGQGANVYLASAELAAVSALLGKLPTPQEYLDYMNNLQKDKDDIYRYMNFDQINSYV
                     KKADNVIVQVAV"
     gene            3748..6105
                     /locus_tag="PWX02_001786"
     CDS             3748..6105
                     /locus_tag="PWX02_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HDL1729780.1"
                     /translation="MNTALEKGKFVFLDKPKNTITTAAHWGALNVTVENGKVVKSEGS
                     LAKNHQNELQDVVADQLYSPVRVKYPMVRKGYLEGNKDTTLRGRDKWVRISWDKAFEL
                     VSGEIQRVREQYGSESIFGGSYGWYSCGSLHASRTLLQRYLNVTGGFTNTKGDYSTGA
                     VQVIMPYVLGNIEVYAQQTSWHTIVESTELLVIWSSNPLNTLRIAWTSAEPEGIDYLA
                     KLKASGKRIICIDPVKSETCQFLEAEWLPINTGTDVALILGIAHTLITENKHDHDFLT
                     KYTVGFEQLADYVLGKADNLPKTAEWASQICGIPEEVIKTLANDFVTHRTMLMSGWGL
                     QRLQHGEQSHWALVTLAAMLGQIGLPGGGFGLNYRYSTNGLNVNSGVRLGAINAGKPN
                     PDMPFFPVSRIADALLKPNQPYDYNGQTLHYPNIKLVYWAGGNPFAHHPDTNYLVKAW
                     QQPETVIVNECYWTPTARMADIVLPATTSYERNDLTVAGDYVAKHIIPMKQVVEPQFE
                     AKNDYDIFAELAKRAGKEQAFTEGKSEMEWLKEFYQVAFEAGEKANTPLLDFEAFWAE
                     NKVLSFEAEHQNNTFVRYQDYRTDPIKHKLATPSGKIEIFSETIAKMNYEDCKGHPTW
                     FEHHEYSSKTNEAEPLALVTPHIKYRLHSQLAYSSLRQYYEVNGREPMLIHTKDAADR
                     GITSGDIVRIFNKRGQVLAGAVVTDGIIQGTVALYEGGWYDPQDLGKTDTPLCKNGNP
                     NVLTRNEGSSKLGQGNAPNTTTVQVEKYVGDAPEVTVFKAPFFSE"
     gene            6260..7771
                     /locus_tag="PWX02_001787"
     CDS             6260..7771
                     /locus_tag="PWX02_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HDL1729781.1"
                     /translation="MTTLFQQFSPTVIKQQWFSNVRGDVLSGLVVALALIPEAIAFSI
                     IAGVDPQIGLYASFCIAVIISFVGGRPAMISAATGAMALVLAGLVRDHGLQYMLAATL
                     LTGVLQIIAGWLKLGSLMRFVARDVVIGFVNALAILIFMAQLPELQNTTWHTYALVVA
                     GLAIIYLFPYVPVIGKIIPSPLVTIIALTLFTLWQGIDVRTVGDMGQLPDTLPVFLLP
                     DIPLNLDTLWIILPYSFSLAAVGLLESLMTATIVDEMTETDSDKNRECKGQGIANIIS
                     GLFGGMAGCAMIGQSVINVKSGGRTRLSTFLAGFLLLIFVVFLSDILKVIPMAALVAV
                     MIMVSIGTFNWQSLEELKTHPIGFNVVMLVTVAIVVYTHNLALGVFSGVLLSALFFAN
                     KIGRYMAVKTSAIEKEELRYDVIGQVFFASSDDFIAQFDFTRSELKKVHIDLTHAHFW
                     DVTAVSALDKVVLKYRKSDVEVEIIGLNEASKTLVDRFGVHDKSEDVDIILNH"
     gene            7878..>8000
                     /locus_tag="PWX02_001788"
     CDS             7878..>8000
                     /locus_tag="PWX02_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDL1729782.1"
                     /translation="MKNKFVAIAAFSGLFTVAFGAIASHALSAQLSPKALGWIET"
BASE COUNT         2242 a         1619 c         1910 g         2229 t
ORIGIN      
        1 ccttagagct tatccgccaa tatcacgtgg cgattaaagg tcctttaatg acaccggtgg
       61 gtggcggtat tcgttcttta aatgtggcga tgcgtcaagg attagattta tacaactgct
      121 tacgcccaat tcgttattat gacggtacgc caagcccggt taaacaccct gagctggtgg
      181 atatggtgat tttccgtgaa aactcggaag atatttatgc cggtgtagaa tgggttgccg
      241 gttcgcctga agccaataaa gtgatcgaat ttttacaaca agagatgggc gtgaaaaaaa
      301 tccgctttac cgaagattgc ggtatcggaa ttaagccggt ttctaagcaa ggcacacaac
      361 gcttggtgcg tgctgcattg caatatgtaa ttgataacaa ccgtaaatcg ctcacgcttg
      421 tgcataaagg taacattatg aaatttaccg aaggagcatt taaagagtgg ggctaccaag
      481 ttgcggaaga atttggggca aaattaattg ataaaggccc ttggatgagc ttgactaatc
      541 cgaaaaccgg tgaggaaatc attataaaag acagcattgc cgatgccttt ttacaagaaa
      601 tcttgttaca cccaaccgaa tatgatgtga ttgctacact gaacctaaac ggcgactata
      661 tttctgatgc tttagccgca caagtaggcg gaatcggtat ttcaccggga gcgaatattg
      721 ggtttgaagc ggctattttt gaagccacac acggcacagc ccctaaaatt gcagggcaaa
      781 ataaaggcaa tcctgggtca ttaattttaa gtggggaaat gatgttacgc catttaggtt
      841 gggttgaagc ggctgattta gtggtaaaag cggtgtctaa aaccattgcc aataaaactg
      901 tcacctttga ttttgcggaa atgctggagg gggtaactct acgctcaacc tcagaatttg
      961 cacaagacat tattgccaat atgtaaagtt ggagagggaa tgatgatgga aaattttata
     1021 gaaaattatc aacaacaggt tgatgaacgt gcggcactgg gtgttgtgcc tcagccactt
     1081 tctgcggaac aaacggctca actggttgaa ttattgaaaa acccaccggc tgaacaagcg
     1141 gtctttttat tggatctttt taccaatcgt attccggcag gtgttgatga agccgcttat
     1201 gttaaagccg cctttttagc cgatattacc aaaggggttg ccacatcgcc gctcatttcg
     1261 cctgctcacg cagtgaaatt actcggcaca atgcaaggtg gttataacat tgaaacctta
     1321 ttgtctgcgt tggataaccc tgagcttgca ccgattgcgg tagaagcctt atctaaaaca
     1381 ttattgatgt ttgataattt ccacgatgta aaagaacggg cagagcaggg taatccgttt
     1441 gcacaacaag tattggaatc ttgggcgaat gccgattggt ttacctctcg cccgaaactt
     1501 gccgaaaaat taacggtaac ggtatttaaa gtcacaggcg aaaccaatac cgatgatctt
     1561 tccccggctc aagacgcttg gtctcgccct gatattccac ttcacgcatt agcaatgctg
     1621 aaaaatgaac gtgacggtat tttccccgat gaaaacggtc ataccggccc gattaagcaa
     1681 ctggaagcat taaaagaaaa aggtttcccg ttagcctatg tcggtgatgt agtcggcacc
     1741 ggctcttctc gtaaatccgc aaccaactcc gtgctttggt ttatggggga agatattcct
     1801 tacattccaa acaaacgtgc cggtggggtg gtgttaggcg gtaaaattgc cccgattttc
     1861 tttaacacat tagaagatgc cggttcttta ccgatcgaag tggatgtaac caacctgaat
     1921 atgggcgatg taatcgacat ttacccttat gcaagcaaaa tttgcaaaca caactccgat
     1981 gaagtcttag ccgaatttga gctaaaaacc gaagtattgc ttgatgaagt gcgtgcaggc
     2041 gggcgaattc ctctgattat cggacgtggt ttgacccata aagccagagt agcacttggc
     2101 ttgcctgaaa gtgatgtatt tattaaaccg cagtcggttt cagagagtaa caaaggctac
     2161 acccttgctc aaaaaatggt agggcgagct tgtggcgtag aaggcgttcg accggggcaa
     2221 tattgcgagc ctcgaatgac gtctgtcggt tcacaagata ccacaggacc aatgacaaga
     2281 gatgagctga aagatttagc ctgtttaggc ttctcggcag atcttgtgat gcaatcgttc
     2341 tgccatactg ccgcttaccc gaaaccggtg gacgtaatta cccaccacac tttgcctgat
     2401 tttattatga accgtggcgg tgtgtcactt cgcccgggtg atggcgtgat tcactcttgg
     2461 ctaaaccgta tgttattgcc ggatactgtc ggtacaggcg gcgattccca cacccgtttc
     2521 ccaatcggga tttcattccc tgccggttcg ggcttagttg cctttgctgc ggcaacaggg
     2581 gtgatgccgt tagatatgcc ggaatcggtc ttggtgcgtt tttccggcac aatgcaaccg
     2641 ggcattacct tacgggatct ggttcacgct attccgtatt atgccattca gcaagacttg
     2701 ctgacggtgg agaaaaaagg taagaaaaat attttctccg gtcgaatttt agaaattgaa
     2761 gggttggaag atctgaaaat cgaacaagcc ttcgagcttt ctgatgcttc tgctgaacgc
     2821 tctgctgcag cctgtaccat caagctcaac aaagagccga ttatcgagta tctgaattct
     2881 aatattacgc ttttaaaatg gatgattgcg gaaggctatg acgataaacg cacgctagaa
     2941 cgccgtatcg ccaatatgca agcgtggttg gataatccgg agcttttaga ggcagacgaa
     3001 aatgcggagt atgccgcagt gattgagatc aacttagatg aaatcaaaga gccgattgtg
     3061 tgcgtaccga atgaccctga cgatgctcgc ctgctttctc aagtacaggg cgataaaatt
     3121 gatgaagtct ttattggttc ttgtatgact aatatcgggc atttccgtgc ggcaggtaaa
     3181 ctgttaagcc aagcacaggg cgaattgccg actaaattat ggattgctcc gccgactaaa
     3241 atggacgcag gcttgctgac cgaagagggc tattacagca tttacggcaa aagcggagca
     3301 agggtagaga tgccgggctg ttctctctgt atgggtaacc aagcccgagt ggcaaataat
     3361 gctaatgtgg tttctacctc tacccgaaac ttcccgaacc gtttagggca aggggcaaat
     3421 gtgtatttag cctcagcaga attagccgca gtttctgcgt tattgggtaa attgccgaca
     3481 ccgcaagagt acctagacta tatgaataac ttgcagaaag ataaggacga tatttaccgc
     3541 tatatgaact ttgaccaaat caatagttat gtgaaaaaag cggacaatgt aatcgttcaa
     3601 gttgccgttt aacttccttg aagcccacct ttttaggtgg gttttgtttt gcaaattttt
     3661 ggctaaaaac aaccgcttgt ggttgcaact tttggcttaa tccagtaaat tcctctcaat
     3721 tttataacag aaaaggcatt aatttaaatg aacacagcat tagaaaaagg caagtttgtc
     3781 tttttggata aaccgaaaaa taccattacc accgccgccc actggggtgc attaaatgta
     3841 acggtggaaa acggaaaagt ggtgaaatca gagggcagtt tagcgaaaaa tcaccaaaac
     3901 gaattgcaag atgtggtggc ggatcagctc tatagcccgg ttcgggtgaa atatccgatg
     3961 gtgcggaaag gctacttgga agggaataaa gacaccactt tgcgagggcg agataagtgg
     4021 gtgagaatct cttgggacaa agcctttgag ctggtttccg gcgaaatcca acgggtaaga
     4081 gagcaatatg gcagcgaatc cattttcggc ggctcttacg gctggtatag ctgtggttca
     4141 ctgcacgcat ctcgaacgct tttgcaacgt tatttaaacg tcacaggcgg ctttaccaat
     4201 actaaaggcg attattccac cggtgcggtg caagtgatta tgccttatgt attaggcaat
     4261 attgaggtgt atgctcaaca aaccagttgg cacacgattg tagaaagcac agaattattg
     4321 gtaatttggt cgtctaatcc actgaataca cttcgcattg cgtggacctc tgccgaaccg
     4381 gaggggattg attatctcgc caaattaaaa gcaagcggta aacggattat ttgtatcgac
     4441 ccggtgaaaa gcgagacctg tcagttttta gaggcagagt ggttgcctat taataccggc
     4501 acggatgttg cactgatttt agggatcgcc cacaccctta ttactgaaaa caagcacgat
     4561 cacgacttct taactaaata tactgttggt ttcgagcaac ttgctgatta tgtgttagga
     4621 aaagcagata atttacctaa aacagcagag tgggcgagcc aaatttgtgg cattccggag
     4681 gaggtgatta aaaccttagc aaatgatttt gttactcacc gcacaatgtt gatgtccggc
     4741 tggggcttgc aacgcctgca acacggggag caaagccact gggcgttagt gactcttgcc
     4801 gctatgctcg ggcaaattgg cttgccgggc ggcggtttcg gcttaaatta tcgttattct
     4861 accaatgggc taaatgttaa ttccggtgtt cgtttagggg caatcaatgc aggtaaacct
     4921 aaccccgata tgccgttttt cccggtgtct cgaattgccg atgcgttgct caaaccgaat
     4981 cagccttatg attacaacgg gcaaacatta cattacccga acatcaaatt agtctattgg
     5041 gcggggggta atccgtttgc tcaccacccg gataccaact atttagtgaa agcgtggcaa
     5101 cagccggaaa cggtgatcgt caatgaatgt tactggacac cgaccgctcg aatggcagat
     5161 attgtattgc ccgcaaccac cagctacgag cgaaacgatc tcaccgttgc cggtgattat
     5221 gtagcaaaac atattatccc gatgaaacag gtggttgaac cgcaatttga ggcgaagaat
     5281 gattacgata tttttgccga attagcgaaa cgagccggta aagaacaagc atttaccgaa
     5341 ggcaaaagcg aaatggaatg gcttaaggaa ttttaccaag ttgcctttga ggcgggggaa
     5401 aaagctaata cgccattgct ggatttcgag gcattttggg cggaaaataa agtgttgagc
     5461 tttgaggcag aacatcaaaa taatactttc gttcgctatc aagattatcg tactgatccg
     5521 attaaacaca aattggcaac gccatcaggc aaaattgaga ttttctccga aaccattgcc
     5581 aagatgaatt atgaagattg taaaggacac ccgacttggt ttgaacatca cgaatattca
     5641 agcaaaacca acgaagccga gccgttggca ttagttacac cgcatattaa ataccgttta
     5701 cacagccagc ttgcttactc ctcattacgt caatattacg aggtaaacgg cagagaacca
     5761 atgctaattc acacaaaaga tgccgctgac agaggcatta caagcggtga tattgtgcga
     5821 atttttaaca agcgagggca ggtgttggca ggagcagtgg tgactgatgg catcattcaa
     5881 ggcacagtag cgttatacga aggtggttgg tatgacccgc aagatttagg caaaacagat
     5941 actccactat gcaaaaacgg caacccgaat gtgctaaccc gtaatgaggg ttcatcaaaa
     6001 ctcgggcagg gcaatgcacc gaacactacc acggttcaag tggaaaaata tgtgggagat
     6061 gcacctgaag tgacggtgtt taaagcccct ttttttagtg aatagcaatt aatccaagcg
     6121 gtcatttttc tactattttt tgcaaaaaaa gagcgaaaga agaccgcttg tttatttgac
     6181 gacttttcac taatttgcta ctctctcgcc tcatttttag ccgactttgc tcggcttttc
     6241 tttttattta agggtaataa tgacaacttt atttcagcaa ttttcgccta cggtaattaa
     6301 acaacaatgg ttttcaaatg tacgaggtga tgttctctct ggtttagtgg tggctttggc
     6361 gttaattcca gaagccatag cattttcaat tattgcaggg gttgatccac aaattggttt
     6421 atatgcctca ttttgtattg cagtaattat cagttttgtg ggcggcagac cggcgatgat
     6481 ttctgcagca accggtgcaa tggctctggt attagcaggc ttagtgaggg atcacggttt
     6541 acaatatatg ctagcggcca ccttattaac aggcgtgttg caaattattg ccggttggtt
     6601 aaaactcggc tcgcttatgc ggtttgtggc acgagacgta gtgattgggt ttgttaatgc
     6661 acttgccatt ttaattttta tggcacaact acctgagtta caaaatacaa cttggcatac
     6721 ttatgcactg gtggtagcag ggcttgctat catttatctt tttccttatg taccggtgat
     6781 tggaaaaatc attccctcac cattagtgac cattattgct ttaacacttt tcacattatg
     6841 gcaaggcatt gatgttcgta ccgttgggga tatggggcaa ttacccgata ccttaccggt
     6901 gtttttatta cccgatattc cgcttaattt agacacctta tggattattt tgccttattc
     6961 tttcagcttg gccgcagtag ggttattgga atctttaatg acggcaacga ttgttgatga
     7021 aatgacagag acagacagcg ataaaaaccg tgaatgtaaa gggcagggca ttgccaatat
     7081 tattagtggc ttatttggtg gtatggcagg ttgtgcgatg attgggcagt cggtcattaa
     7141 tgttaaatcc ggtggacgta caagactatc tacctttctc gccggttttt tgttactgat
     7201 ttttgtggta tttttgagcg atattctgaa agtgattccg atggctgcat tagttgcggt
     7261 gatgattatg gtctctatcg gcacctttaa ttggcaatcc ttagaagagc taaaaacgca
     7321 cccaatcggc tttaatgtag tgatgttagt caccgttgcg attgtggttt atacacataa
     7381 cttagccctt ggcgtatttt ccggcgtatt actctcggca ttattctttg cgaataaaat
     7441 tggacgttat atggcggtta aaacaagtgc tatagagaaa gaggaattgc gttatgatgt
     7501 tatcgggcaa gtattttttg cctcatctga tgattttatc gcacaatttg attttacccg
     7561 ttcagaactg aaaaaagtcc acattgattt aacccacgct cacttttggg acgtaacggc
     7621 agtttcagcg ttagataaag ttgtgttgaa atatcgaaaa agtgatgttg aggtagaaat
     7681 tatcggatta aatgaggcca gcaaaacctt agtagataga tttggggttc acgataaatc
     7741 ggaagatgtg gatattatct tgaaccatta agcatacaag cggtgatttt ttgtaatttt
     7801 tttgcaaaat ttcaccgctt gttggtttcg ataatatgat atagtaagcc atctaacatc
     7861 atacctatct tcaacttatg aaaaacaaat ttgtagcaat tgccgcattc agcggcttat
     7921 ttactgtggc atttggggcg atagccagcc acgcattatc ggctcaatta tcgcctaaag
     7981 ccttaggctg gattgagacg
//
LOCUS       DANJWC010000116         7922 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.116, whole genome shotgun sequence.
ACCESSION   DANJWC010000116 DANJWC010000000
VERSION     DANJWC010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7922
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.116"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..815)
                     /locus_tag="PWX02_001789"
     CDS             complement(<1..815)
                     /locus_tag="PWX02_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDL1729783.1"
                     /translation="MNLRWQEYPENARKLFKLSLPIFISQLSASGMGLADIVMAGLVS
                     DDDVSAIAVSNSIYFPLFLFVLGLLNAITPTVSYLNGSSNRHLIANQVRQGYWIVFAM
                     SIPLMAIFLNSHLILDLMQTPAEFSIKSQQYLAVMAVGLIPALLAVSLRCMNDGLSNP
                     KPAMRITFFGLLLNIPLNYIFIFGKFGLPEMGAVGCGVATAIVNWIMFFMLLHYCYTN
                     SAQKDIRLFEKWIDAPSGQILLKLCKLGLPIAFAIFTEVMLFSTSSLFLSPLGS"
     gene            complement(951..2045)
                     /locus_tag="PWX02_001790"
     CDS             complement(951..2045)
                     /locus_tag="PWX02_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL1729784.1"
                     /translation="MKKLAGLFAAGLATVALTACNEEKAAEPKATAAEAKPQATNQTV
                     HLYTWTEYVPEGLLDEFTKETGIKVEVSSLESNETMYAKLKLQGKDGGYDVIAPSNYF
                     VSKMAKEGMLAELDHAQLPVIKELNQDWLNKPYDQGNKYSLPQLLGAPGIAFNTADYK
                     GETFTSWGDLWKPEFAGKVQLLDDAREVFNIALLKLGKNPNTTNPDEIKEAFEELKKL
                     RPNVLSFTSDNPANSFIAGEVSVGQLWNGSVRIAKKENAPVDMVFPKEGPVLWVDTLA
                     IPANAKNKENAHKLINYLLSAKIAEKLTLAIGYPTSNVEALKVLPKEITEDIAIYPSA
                     EVLQKSQWQDDVGDAIELYEKYYQELKAAK"
     gene            2267..2863
                     /gene="grpE"
                     /locus_tag="PWX02_001791"
     CDS             2267..2863
                     /gene="grpE"
                     /locus_tag="PWX02_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDL1729785.1"
                     /translation="MTTQNEHLQEEVKQEEIQPEVVTEETQAEEQNTPQEEPLALAQA
                     RIAELEAYIAEADKREQDIQLRAQAEIQNIRRRAEQDVEKAHKFALEKFSKELLTVVD
                     NLERGLDALDKAVTDETTQALVDGVEMTHKEFINTLAKFGVVAIGEVGEAFNPELHEA
                     ISMQPAEGIEANHVSTVLQKGYTLHGRVIRPAMVMVAN"
     gene            2961..3230
                     /locus_tag="PWX02_001792"
     CDS             2961..3230
                     /locus_tag="PWX02_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729786.1"
                     /translation="MDAAMSLRFDKLRFVKRLQEANQTPEMAEAFANALDEALEQSQS
                     PLATKADLQLELEKLKNEINTTIFKAITLNITILGFLMAIMKFIN"
     gene            3293..3745
                     /locus_tag="PWX02_001793"
     CDS             3293..3745
                     /locus_tag="PWX02_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDL1729787.1"
                     /translation="MTLQFNAVALLLVVLIILGLISQNSAVTISAAVLLIMQQTLLSK
                     FVPFVDQYGLKIGIIILTIGVLSPLVSGRITLPELAQLLNWKMALSIIAGVLVAWLGG
                     RGVNLMGSQPVLVTGLLIGTVIGVAFLKGVPVGPLIAAGILSLIVGKT"
     gene            3849..7835
                     /gene="hrpA"
                     /locus_tag="PWX02_001794"
     CDS             3849..7835
                     /gene="hrpA"
                     /locus_tag="PWX02_001794"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HDL1729788.1"
                     /translation="METPLQKNHKKPPLVKLENITTADFRRLLGRLKGAENIKNIDHK
                     RSVLAEISQDLTACQARFFARTQHAQNLKIDYPDLPVSARREEILKLISENQVVVIAG
                     ETGSGKTTQLPKMCLELGRGVKGLIGHTQPRRIAARSVATRIAEELKSELGETVGYKV
                     RFNDQVSENSLIKLMTDGILLAEIQLDSYLNQYDTLIIDEAHERSLNNDFILGYLKQI
                     LHKRPDLKVIITSATIDVERFSKHFNNAPIIEVSGRTFPVEVRYRPIVEDQDQLQGIL
                     NAVDELQAEGRGDILIFMNGEREIRDTAEALQKQELRHSEILPLYARLSAAEQQRIFQ
                     PSGGLNRIILATNVAETSLTIPNIKYVIDTGTARISRYSYRTKVQRLPIEPISQASAN
                     QRKGRCGRTSEGICIRLYSEEDFNSRPQFTDPEILRTNLAAVILQMTSLGLSDIANFP
                     FVDAPDSRQIQDGIRLLEELQAIIPNEKIPSTACGGGLGRGEIRSNDGTPPLPTSPRK
                     RGDGFRLTPIGRQLAQLPVDPRLGRMVIEAAKNGSLHEVMMIVSALSIQDPRERPQEK
                     QQSADDKHRRFADKESDFLAFVNLWHFIQAQQKELSKNQFRNLCRKDYLNYNRVREWQ
                     DIYHQLRLAVREMGLKINSEPASYQPIHTALLTGLLSHIGLKDNEKMHYLGARNAHFF
                     IFPNSALFKKQPKWVMAAELVETTKLWGRMVASIEPEWVEPLAVHLTKSSYSEPHWAK
                     SKGAVMASEKVSLYGIPIVAQRSVNYGSIDPTVSREIFIRSALVEGECHNNYKFFKEN
                     HRLIREVEALEHKSRRRDILVDEQVLFEFYDQRIGTEVVSSKHFDTWWKQASKKDPEL
                     LNFEKSFLINDNANLASELDFPNFWHQGDLKLKLSYQFEVGKDHDGVTVHIPLPLLNQ
                     VEPEGFDWQIPGLRHELIVSLIKSLPKSLRRNFVPAPNYADAFLGRAEPFKKPLLDSL
                     SHELRRMTGVLVEPEQWDLSQLPPHLRMTFRVIDDKGKKLQESENLDELKFALKDEVQ
                     NTLSNIADDGIEQSGVHIWNFAELPQFYEQKKAHFSVKAYPAIVDEQTAVGVKLFETE
                     FEQARAMQAGLRRLLLLNVPSPIKYLHEKLPNKAKLGLYFAPFGKVLELIDDCIACAV
                     DQLVEEFGGFVWTEEKFTQLHEFVRANLNDKTVEIAKQVEKCLTLAYELNKRLKGKLD
                     FTMAFALSDIKTQIEGLIYPDFVTKTGYARLADLYRYLTAIERRLDKLLVDSNTDRAK
                     MLRVEAVQEAYKQLLAKLPKSKAVPDEVLEIRYMIEELRVSLFAQQLGTKYPISDKRI
                     LNVINEIK"
BASE COUNT         2337 a         1744 c         1745 g         2096 t
ORIGIN      
        1 gaaccaagtg gtgacagaaa cagcgatgag gtcgaaaaca gcatcacttc ggtaaaaatc
       61 gcaaaggcaa tcggcagccc taatttgcaa agttttagca aaatttgacc gcttggagca
      121 tcaatccatt tctcaaacaa acgaatatct ttttgggcag aattggtgta gcaataatgc
      181 aacaacataa aaaacataat ccagttcaca attgcggttg ctacaccaca gcctaccgct
      241 cccatttcag gcaagccaaa tttgccgaaa atgaaaatat agtttagcgg aatgttcagc
      301 aataaaccga aaaaggtaat ccgcatcgca ggtttagggt tagataatcc atcgttcata
      361 caacgtaaac tcaccgccag caaagcagga ataagcccaa ctgccattac tgctaaatat
      421 tgttgcgatt tgattgaaaa ctcagccggt gtttgcatta agtctaaaat cagatggctg
      481 tttaggaaaa ttgccattaa tggaatagac attgcaaaca caatccaata gccctgcctt
      541 acttggttag caattaaatg gcgattgctc gagccgttta agtaagaaac ggttggggta
      601 attgcattca gcaaccctaa cacaaacaga aagagcggaa agtaaatcga attacttacc
      661 gcaatcgccg agacatcatc atcactcacc aagccagcca ttacaatatc cgccagcccc
      721 attccggaag ctgaaagctg ggaaataaaa atgggtaggg atagtttaaa taatttgcgg
      781 gcattttcag gatattcttg ccatcttaag ttcatggttt aatctctcaa aatctaagta
      841 tgtttttatg attgtacctt agaaagcggt cgtttttgcc aaattttttg caactcaaaa
      901 gagaaagcct ctaaagattg ctccctagag gcttcagaat taatcagcta ttattttgct
      961 gcttttaact cttggtagta tttttcgtaa agctcaattg catcaccaac atcatcttgc
     1021 cattgagatt tttgtaaaac ctctgcagat gggtaaattg ctatatcttc agtaatttct
     1081 ttcggtaata cttttaatgc ttctacgttt gaagttgggt aaccaatcgc taacgttaat
     1141 ttttcagcta tttttgcact taataagtag ttgattaatt tatgtgcatt ttctttgttt
     1201 ttcgcatttg ccggaatggc taaagtatcg acccaaagca caggaccttc tttcgggaat
     1261 accatatcaa ccggtgcgtt ttctttcttc gcaatacgta cagaaccgtt ccataactga
     1321 cctacagaaa cctcaccagc aataaatgag tttgccgggt tatcagaggt aaatgaaagc
     1381 acatttggac gtaatttttt caactcttca aaggcttctt tgatttcatc cggattagtt
     1441 gtatttgggt ttttacctaa ttttaataac gcaatgttga atacttcacg agcatcatct
     1501 aataattgta ctttacctgc aaattcaggt ttccacaaat caccccaaga agtgaatgtt
     1561 tcacctttgt aatctgccgt attaaatgca atacccggcg cacctaataa ttgaggtaat
     1621 gaatatttgt ttccttgatc gtaaggttta tttaaccaat cttggtttaa ttctttaata
     1681 accggaagtt gtgcgtgatc taattccgct aacattcctt ctttcgccat tttagataca
     1741 aagtagtttg atggagcaat tacatcataa ccgccatctt tgccttgtaa ttttaattta
     1801 gcgtacattg tttcgttaga ttcaagactt gaaacttcaa ctttaatgcc ggtttctttc
     1861 gtaaattcat ctaacaaacc ttcaggcaca tattcagtcc aagtataaag gtgaactgtt
     1921 tgatttgttg cttgaggttt tgcttcagcc gcagttgctt ttggctctgc cgctttttct
     1981 tcattacacg ctgttaatgc aactgttgct aaacctgctg caaataaacc cgctaatttt
     2041 ttcatttaag ttttctccgt taaagaaaag tgattaaaaa atatagcctt gcctaagggc
     2101 ggaaaagact agggcatttt aagccccccc ccgtattttg caagcctttt ctgattaaaa
     2161 agtgaattat tttcgcaaaa agaaagagat atgcttgaaa tcccttaatt tatcgctata
     2221 ttaaggaaga tttacatttc cagattagtc ttttaaagga taaaaaatga ccactcaaaa
     2281 tgaacactta caagaagaag taaaacaaga agaaattcaa cctgaagttg taactgaaga
     2341 aactcaagcg gaagaacaaa atacacctca agaagaaccg ttagctctcg ctcaagctcg
     2401 aattgccgag ttagaagcct atattgcaga agccgacaaa cgtgagcaag atattcagct
     2461 tagggctcaa gcagaaattc aaaatatccg ccgccgtgca gagcaagatg ttgaaaaagc
     2521 tcacaaattc gctttagaaa aattctccaa agaattgtta acagtggttg ataaccttga
     2581 acgtggatta gatgcccttg ataaagccgt gaccgatgaa accactcaag cgttagttga
     2641 cggcgttgaa atgactcata aagaattcat caatacactg gctaaatttg gtgtggtggc
     2701 aattggcgaa gtaggcgagg catttaaccc tgaattgcac gaagctattt caatgcaacc
     2761 tgccgaagga attgaagcca accatgtcag cacagtgcta caaaaaggct acacgcttca
     2821 cggacgagtg attcgcccgg caatggtgat ggtggcaaac taaattgtcg attttcgacg
     2881 aagatcacaa aatctcctaa aattcctgct tgtgattatg ctaaattagt ttataactta
     2941 accacaagag gataattgaa atggatgcag ctatgagcct acgatttgac aaattacgtt
     3001 ttgtaaaacg cttgcaagaa gccaatcaaa cacctgaaat ggctgaggca tttgctaatg
     3061 ctctcgatga agccttagag caaagtcaaa gcccactagc aacaaaagct gatttgcaac
     3121 ttgaacttga aaagctaaaa aatgaaatca ataccacgat atttaaagca attaccttaa
     3181 atatcacaat tcttggcttt ttaatggcaa taatgaaatt tattaactag ctcaacgttt
     3241 aattaaccac tcccttactt ctatgaagta agggttttta cgttttaatc ctatgacttt
     3301 acaatttaac gcagtggcac ttctacttgt ggtgctgatt attttagggc tgatcagcca
     3361 aaacagtgcg gttactattt cagccgccgt attgctgatt atgcaacaaa ccttactttc
     3421 taaatttgtc ccctttgtcg atcaatacgg cttaaaaatc gggattatca ttttaaccat
     3481 cggcgtactt agccccttgg tttcagggcg aattacgctt cccgaacttg cccaactatt
     3541 gaactggaaa atggcactct caataattgc tggcgtgctg gtagcgtggc taggtggcag
     3601 gggcgtgaat ttaatgggca gccagccggt gttggtaact ggacttttaa tcggcacggt
     3661 gatcggcgtg gcgttcttaa aaggcgtacc ggtcgggccg ttgattgcgg cagggatttt
     3721 gtcactgatt gttggtaaaa cctaatgtag ggtggacgta agtccacgca taaaatccct
     3781 attctgttac gtgggcttac gcccacccta caataaatca caaggtctta atgcaaaaga
     3841 gaaattaaat ggaaacacct ttgcaaaaaa atcataagaa accaccgctt gtgaagctgg
     3901 aaaatatcac caccgccgat ttccgccgcc tgctcggtcg cttaaaaggg gcggaaaata
     3961 ttaaaaacat cgatcacaag cggtcagttt tggcagaaat ttcgcaagat ctcaccgctt
     4021 gtcaggcacg ttttttcgca cgaactcagc acgcccaaaa cctgaaaatt gactaccctg
     4081 atctgcctgt ttccgctcgc cgtgaggaaa ttttaaaact gattagcgaa aatcaggtgg
     4141 tggtgatcgc aggggaaacc ggttcgggca aaaccaccca gttaccgaaa atgtgcttag
     4201 aactcgggcg tggggtgaag ggcttgattg ggcataccca gccccgccga attgccgcac
     4261 gttcggtggc gactcgcatt gccgaagagc tgaaatcgga actgggcgag acggtcggtt
     4321 acaaagtccg ctttaacgat caagtcagtg aaaacagctt aatcaagcta atgacggacg
     4381 gtattctgct ggcggaaatc cagctcgaca gctatttaaa tcagtacgat acgctgatta
     4441 ttgacgaagc tcacgagcgt tcgctgaaca acgattttat tttgggttat ctgaaacaaa
     4501 ttctgcacaa acgccccgat ctgaaagtga tcatcacctc ggcaactatt gatgtagaac
     4561 gtttttccaa acatttcaac aacgccccga ttattgaagt ctctggtcgg acattcccgg
     4621 tggaagtgcg ttatcgcccg atagtggaag atcaagatca actgcaaggt attctaaatg
     4681 cggtggacga actgcaagcc gaagggcgtg gcgatattct gatttttatg aacggtgaac
     4741 gggaaattcg tgacactgcc gaagccttgc aaaagcagga actgcgacat agcgaaattt
     4801 taccccttta cgcccgctta tcggctgccg aacaacaacg gattttccag ccaagcggtg
     4861 ggctaaaccg aattattctg gcgaccaacg tggcagaaac ctccctcact attccgaata
     4921 ttaaatatgt gatcgacacc ggcacggctc gtatttctcg ttatagctac cgcaccaaag
     4981 tgcaacgctt gccgattgag ccgatttcgc aagcctccgc caaccagcgg aaagggcgtt
     5041 gtgggcgaac ctcggaaggg atctgtatcc gcctctattc agaagaggat ttcaacagcc
     5101 gtccacaatt taccgatcct gaaattctac gaactaacct tgcagcggtt attttacaaa
     5161 tgacttcgct tggattgagt gatattgcca atttcccgtt tgttgatgcc cctgacagcc
     5221 gacaaattca ggacgggatt cggttgttgg aagaattaca ggcgattatt ccaaatgaaa
     5281 aaatcccctc caccgcttgc gggggagggc tagggagggg ggaaatccgt tctaatgatg
     5341 gcactccccc cctcccaacc tccccccgta aacgggggga tgggtttaga ttaaccccaa
     5401 tcggtcgcca actcgcccaa ctgccagtcg atcctcgctt aggcagaatg gtgatcgagg
     5461 cggcgaaaaa cggttcgttg cacgaagtga tgatgattgt gtcagctctc tcaatccaag
     5521 accctcgtga gcgtccgcag gaaaaacagc agtcggcaga cgataaacac cgccgttttg
     5581 ccgataaaga gagcgatttc ttagcctttg tgaatttgtg gcactttatc caagcccaac
     5641 aaaaagaact gtcgaaaaac cagttccgta atctctgccg taaggattat ttgaactaca
     5701 accgtgtgcg ggagtggcag gatatctacc accaactccg ccttgcagtt agagaaatgg
     5761 gcttaaaaat taacagtgag ccggcaagtt atcagccgat tcacaccgcc ttgctcactg
     5821 gcttgttgtc gcatatcggc ttgaaggata atgaaaaaat gcactattta ggggcaagaa
     5881 atgcccactt tttcattttc ccgaactcgg cacttttcaa aaaacagccg aaatgggtaa
     5941 tggcggcaga gctggtggaa accactaaac tttggggtcg aatggttgct agtattgagc
     6001 cggaatgggt ggagccgttg gcagtacacc tcactaaatc cagttacagc gaaccgcact
     6061 gggcgaagtc aaaaggggcg gtaatggcat cggaaaaagt gtcgctctac ggcattccga
     6121 ttgtggcaca acgctcggtg aattatggct cgatagaccc aaccgtcagc cgtgagattt
     6181 ttatccgctc ggcgttggtg gaaggcgaat gtcacaacaa ttacaaattt ttcaaagaaa
     6241 atcaccgctt gatccgtgaa gttgaggcat tggaacacaa atcccgtcgc cgtgatattt
     6301 tggtggacga gcaggtgctg tttgagtttt acgatcagcg gattggcacc gaagttgtgt
     6361 cgagcaagca ttttgatact tggtggaaac aggcaagcaa aaaagatcct gaactgctga
     6421 actttgaaaa atcgttcctg atcaacgaca acgccaacct tgccagcgag ctggatttcc
     6481 cgaatttctg gcatcaaggc gatttaaaac tcaaactgag ctatcagttt gaggtcggaa
     6541 aagatcacga tggcgtgacg gtgcatattc cgctaccgct gcttaaccaa gtggaacccg
     6601 aaggtttcga ttggcaaatt ccggggctac gccacgagct gatcgtctcg ctgattaaat
     6661 ctttaccgaa atcgctacgg cgaaattttg tgccggcgcc gaattatgcg gatgcctttt
     6721 tagggcgagc cgagccgttt aaaaaaccgt tgctggacag tctaagccac gaactacgcc
     6781 gaatgacggg tgtattggtc gagcctgaac agtgggattt aagccaactg ccgccacatt
     6841 tgcggatgac tttccgtgtg attgatgata aaggcaagaa actgcaagag agcgaaaacc
     6901 ttgatgagct gaaatttgcc ttgaaagatg aggtgcagaa caccctttcc aacattgccg
     6961 atgacggcat tgagcaaagt ggtgtgcata tttggaattt tgccgaattg ccacagttct
     7021 atgagcagaa aaaagctcat ttcagcgtga aagcctaccc tgcgattgtc gatgaacaaa
     7081 cggctgtggg ggtgaaactg tttgaaacgg aatttgagca ggctcgtgca atgcaggcag
     7141 gacttcgccg tctgctgttg ctaaatgtgc catcgccgat taaatatcta cacgaaaaac
     7201 tgccgaacaa agccaagctg ggcttatatt ttgccccgtt cggcaaagta ttggagctga
     7261 ttgacgactg cattgcctgt gcggtagatc agctggtaga agagttcggc gggtttgtct
     7321 ggacggagga aaaattcacc caactgcacg aatttgtgcg ggcaaattta aacgataaaa
     7381 ccgtagaaat tgccaaacag gttgaaaagt gcctcacact ggcctacgag ctaaacaagc
     7441 ggttaaaagg caagctcgat tttacgatgg cgtttgcgtt aagcgacatc aaaacccaaa
     7501 tcgaagggct gatttacccc gactttgtca ccaaaaccgg ctacgcccgc cttgcagatt
     7561 tataccgtta tttgaccgct atcgaacgcc gtttggataa actgttagtg gacagcaaca
     7621 ccgaccgagc caaaatgcta cgggtggaag cagtgcaaga ggcatataaa cagctccttg
     7681 ccaaactgcc aaaatccaaa gcggtgccgg acgaggtact ggaaattcga tatatgatcg
     7741 aagaactgcg ggtcagtctg ttcgctcaac agctcggcac aaaatacccg atttcggata
     7801 agcggatttt gaatgtgatc aatgaaataa agtaatattc cctatacaac aagcggtcag
     7861 ataagtaaaa aattttgcaa ctctgaccgc ttgttttctc gaatacctat tcctctcgct
     7921 tc
//
LOCUS       DANJWC010000117         7775 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.117, whole genome shotgun sequence.
ACCESSION   DANJWC010000117 DANJWC010000000
VERSION     DANJWC010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7775
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.117"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(61..135)
                     /locus_tag="PWX02_001795"
     tRNA            complement(61..135)
                     /locus_tag="PWX02_001795"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(101..103),aa:Gln,seq:ttg)
     gene            complement(161..245)
                     /locus_tag="PWX02_001796"
     tRNA            complement(161..245)
                     /locus_tag="PWX02_001796"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(209..211),aa:Leu,seq:tag)
     gene            complement(248..324)
                     /locus_tag="PWX02_001797"
     tRNA            complement(248..324)
                     /locus_tag="PWX02_001797"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(288..290),aa:Met,seq:cat)
     gene            complement(491..1315)
                     /gene="aroE"
                     /locus_tag="PWX02_001798"
     CDS             complement(491..1315)
                     /gene="aroE"
                     /locus_tag="PWX02_001798"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDL1729789.1"
                     /translation="MNRYAVWGNPIAQSKSPRIHQLFALQTQRNIEYIANLGDETAFE
                     QQLADFFASGAKGCNITAPFKERAFKLADEFSETALLAQACNTLKRLEDGRLYADNTD
                     GAGLVSDLERLGWLQKGHKTLILGAGGATKGVLLPLLKAGLEITLYNRTQEKAVVLAE
                     KFAKFGKIQTACLSDLANQRFNLIVNATSLGLQGKYVELPESVIASANIYDMQYAPNM
                     QTPFLNYARRCGATACQDGLGMLVGQAAFAFSLWEGQFPDVEPVLAQLKLEMQTVK"
     gene            complement(1322..1876)
                     /locus_tag="PWX02_001799"
     CDS             complement(1322..1876)
                     /locus_tag="PWX02_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HDL1729790.1"
                     /translation="MNNFLEIVNQLKQEEVVAYPTEAVFGLGCNPQSEKAVRKLLVLK
                     QRPEEKGLILLASDLKYLLPYIDEARMTEQAWQRLSHIGEQAITWIVPVKNTVPSYLT
                     GKFDSIAVRLCNVPAVVQLCEASGVALTSTSANLSGEPPCRNAKEVIDQFGQDFPVLH
                     ADTLGRENPSEIRDIFSQQIFRQG"
     gene            complement(1869..2405)
                     /locus_tag="PWX02_001800"
     CDS             complement(1869..2405)
                     /locus_tag="PWX02_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HDL1729791.1"
                     /translation="MVLFKSTKQTETCPQCGSPLQIKQGKKGLFLGCSAYPECDYIKP
                     LHQAGHIIKQLDELCPECGGNLQLKQGNYGIFIGCSHYPECHFIVHEEAEVEEEFDCP
                     ECKQHKLIARKGRSGKIFYGCIGFPNCKFTLATRPINHLCEKCGCELATIKKIKGKQF
                     TICANKSCQHQTELKPNE"
     gene            complement(2415..2726)
                     /locus_tag="PWX02_001801"
     CDS             complement(2415..2726)
                     /locus_tag="PWX02_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDL1729792.1"
                     /translation="MNREKITFFSRFEADILSGKKTITIRDKSESYFQPNQELAVFTN
                     ETDRFFANIKVLSVTPIVFDELNEQHAKQENMSLAELKQVIREIYPNDNAFFVIEFTL
                     I"
     gene            complement(2719..3195)
                     /gene="recX"
                     /locus_tag="PWX02_001802"
     CDS             complement(2719..3195)
                     /gene="recX"
                     /locus_tag="PWX02_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDL1729793.1"
                     /translation="MTTSTTKTNKYTAVNYLLYLLSKRDYSEQELRQKLKQKEYELTE
                     IDAAIEKAQANQWQSDERFCTTFIRYRSMQGIGPRRLKQELKLKGVKDWLINQELENA
                     EVDWFELAEQIFEKKRPLVWDLKAKQKMWRFMVSRGFYNDHFSHLMALDYSSDEYE"
     gene            complement(3254..4360)
                     /gene="recA"
                     /locus_tag="PWX02_001803"
     CDS             complement(3254..4360)
                     /gene="recA"
                     /locus_tag="PWX02_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDL1729794.1"
                     /translation="MAEKKSQKNTPVKQIDPEQKEKALAAALAQIEKQFGKGSIMKLG
                     DTQALDIEAVSTGSLGLDSALGIGGLPMGRIVEIYGPESSGKTTLTLSVVAQAQKNGK
                     TCAFIDAEHALDPIYARKLGVDTDGLLISQPDNGEQALEICDALVRSGAVDVIIVDSV
                     AALTPKAEIEGDMGDSHMGLQARLMSQALRKLTANIKATNCLVIFINQIRMKIGVMFG
                     NPETTTGGNALKFYASVRLDIRRSGVVKDGDEVIGSETKVKIVKNKVAPPFREVQFDI
                     MYGEGIARMNELLILAESHGFINKAGAWFSYEGEKIGQGKNNAIKWLKEHPEVASKIE
                     QDIRNLLISNPTFTATPDSENADNADDEFSEEEL"
     gene            complement(4461..5081)
                     /locus_tag="PWX02_001804"
     CDS             complement(4461..5081)
                     /locus_tag="PWX02_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HDL1729795.1"
                     /translation="MCRFPLFETLSIIDGKVQNLQYHQQRFEQATQEYFGCEPRFSLA
                     DILVVPEASQRGKVRCRIDYNATEFELKFFIYSPKKVERFRLVEVENWDYHLKFSDRK
                     RFDLLPILQNEEVIILNNGYVSDCSIGNLLFLKQGIWYSPQHYLLKGTQLSKLLDEGK
                     VVLAKIAKQDLHQYEKIMLINALNPFEEPRALPCSQIYFCDLDRKS"
     gene            complement(5059..6033)
                     /locus_tag="PWX02_001805"
     CDS             complement(5059..6033)
                     /locus_tag="PWX02_001805"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HDL1729796.1"
                     /translation="MQDFIHQANHYGQAKRPFFFLIDFEQKKPLIFPLEQAHASGLFF
                     EFFCKSNLEKPVKKPTKAFELSANPIPFSEYQTGFELVKKEIQAGNSYLLNLSYPTAI
                     ETNYSLAEIFAASKAKYKCYLQGRFVCFSPECFVRIENNRIFSYPMKGTINANEENAE
                     QKLMASEKEFTEHNTIVDLIRNDLALVAKNIEVTKYRYVEKVETHRGAILQTSSEICG
                     ELGENWQENIGSILAKLLPAGSISGAPKEKTVSIIQQAELGKRGYYTGIFGYFDGESL
                     ESAVAIRYIAQTSNGLVFHSGGGITSQSVLTDEYNELLEKVYVPISAI"
     gene            6143..7750
                     /gene="rmuC"
                     /locus_tag="PWX02_001806"
     CDS             6143..7750
                     /gene="rmuC"
                     /locus_tag="PWX02_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HDL1729797.1"
                     /translation="MHFQEIGIYIALAVCVLVGVYLFFLKSRYQRDAYELSQDLGKIT
                     ENFEQIQQKYEVLAQEKNQLEQWAIQQQTKYEAVSERLNERDGQLQRFQQKIELAEQQ
                     ENQLERYINELKERVGSSQAKAESLEEQLQFSQSSLSTKERENQALFSRLNEVQNELT
                     ELRTTLSEKQANFEAQQRNFIEVKQQLNVEFQHLAQQILDEKSKRFSETNQSSLEALL
                     KPFKEQIEGFQKRVNEVHSESLKGSANLEAEIKRVLQIGVSMSEEAQNLATALKGNNK
                     IAGNWGEVQLESALQSAGLLAGEHYQAQESFRDEEGRRFAPDFVVHLPDQKHLIIDSK
                     VSLVAYDQAVRSEENFAISQALDEHCRSLRNHIEGLSKKNYSALLGIKSPDFVLMFIP
                     IEPAYIEAMKHDPQLFNFGYERNVILVSHTTLMPILRTVANLWRIERGNSEAREISEK
                     AGEIYNQVCAVADRLAKLGNTLNTANNQYNQTVTSLVGRQGLLGKVERFQHLSSKASQ
                     IVPQVELIENEVDTIKLEMVSRELLEE"
BASE COUNT         2281 a         1627 c         1507 g         2360 t
ORIGIN      
        1 accccaaaag aaaaaactaa cttacgttag cttatataga tttgaatgtt tgaaattaag
       61 tggctggggt actaggattc gaacctaggg atgccgagat caaaacccgg tgccttaccg
      121 cttggcgata ccccaacact aatttttgtt aatcgataaa tggtgcggga cgagggactt
      181 gaacccacac acctcgcggc gccagaacct aaatctggtg cgtctaccaa tttcgccagt
      241 cccgcaatgg tggctacagc gagattcgaa cttgcgaccc caacattatg agtgttgtgc
      301 tctaaccagc tgagctatgt agccatttgc gttcaccata tcggtcaacg gagtgcatta
      361 tgctgatttt agggctttcc gtcaaatact ttttttaaaa aatttgaatt tttgattcgc
      421 ttgtatagtt attcgtcgtt ttggcttaaa tctagcattt tttgtttata aaataatagc
      481 ttatattctg ctatttcacc gtttgcattt ctaattttaa ttgagccagt acaggctcaa
      541 catcggggaa ttgcccttcc cacaaagaaa acgcaaacgc tgcctgtcca accaacattc
      601 ctaatccgtc ttggcacgct gttgctccac aacgccttgc ataattgaga aaaggagttt
      661 gcatattggg ggcatattgc atatcgtaaa tattcgccga tgctataaca gattcaggca
      721 attcaacata tttgccttgc agaccgagag aggtggcatt gacgattaaa ttaaaccgtt
      781 ggtttgccaa atcgcttaaa caagcggtct gaattttgcc aaattttgca aatttttcgg
      841 ctaaaacgac cgctttctcc tgcgtgcggt tatatagagt aatctctaac cccgctttaa
      901 gtaacggaag taatacgcct ttggttgctc cacctgctcc aagaattaag gttttatgcc
      961 ctttttgtaa ccaacctagc cgttccaaat cagaaactaa accggccccg tcggtattat
     1021 cggcatataa ccgtccatct tctaagcgtt taagcgtatt acacgcctgt gccaacaaag
     1081 cggtttcgct aaattcatct gccaatttaa aggctctttc tttaaaaggg gcggtgatgt
     1141 tgcaaccttt tgctccagaa gcgaaaaaat cggctaattg ttgctcaaaa gccgtttcat
     1201 ctcctaagtt agcgatatat tcaatgttgc gttgggtttg taaagcaaag agttgatgaa
     1261 tacgggggga tttactttga gcaatcgggt tgccccacac ggcatatcgg ttcatttcgg
     1321 cttatccttg tctaaaaatc tgttggctga aaatatctcg aatttcggaa gggttttctc
     1381 tacctagcgt gtctgcgtgt aatacaggaa aatcttgacc aaactgatcg atgacttctt
     1441 ttgcattgcg gcaaggtggt tctccgctca aattggcact ggtagaggtc aacgcaacgc
     1501 cgcttgcctc acacaactga accaccgccg gcacattaca taaacgtacg gcaatagaat
     1561 caaatttgcc agtgaggtaa cttggcacag tatttttaac cggtacgatc caagtaattg
     1621 cctgctcgcc aatatggctt aggcgttgcc aagcctgttc cgtcattcga gcttcatcaa
     1681 tatagggaag aagatacttt aaatcagaag ccagtaaaat tagccctttt tcctcagggc
     1741 gttgttttaa tacgagtaat ttccttaccg ctttttcact ttgtggattg cagcctaagc
     1801 caaaaaccgc ttcggttggg taagctacaa cttcttcttg ctttaattga ttaactattt
     1861 ctaaaaaatt attcattcgg ttttaattct gtttggtgct ggcaactttt gttagcacaa
     1921 atggtgaatt gcttgccctt aatttttttg attgtagcga gttcacaacc acatttttca
     1981 cacaagtggt taatcggtcg agttgctaat gtaaatttgc aattagggaa gcctatacaa
     2041 ccataaaata ttttgcctga acgccctttt ctagcaatca gtttatgttg cttgcattca
     2101 gggcaatcaa actcttcttc aacctcagct tcttcgtgta cgatgaagtg gcattcaggg
     2161 taatggctac agccgataaa aataccataa ttgccttgtt ttaattggag gtttccgcca
     2221 cattcagggc aaagttcatc taattgctta ataatatgac cggcttgatg taatggctta
     2281 atataatcac attcagggta agctgaacaa cccaaaaata accctttttt gccctgctta
     2341 atttgcaacg gcgagccaca ttgtgggcaa gtttcggtct gtttggttga tttgaaaagt
     2401 accatattga tttgctaaat aagcgtaaat tcaatcacaa aaaacgcatt gtcattcgga
     2461 taaatctccc gaataacttg tttcaattcg gctaagctca tattttcttg tttagcgtgt
     2521 tgctcgttta attcatcaaa cactataggt gttaccgata gtactttaat attggcgaaa
     2581 aatctatcgg tttcattagt aaaaaccgct aattcttggt ttggttgaaa ataagattct
     2641 gacttgtctc gaatcgtaat tgttttttta ccgcttaaaa tatcggcttc aaatcggctg
     2701 aaaaaggtaa ttttctctct attcatattc atcgctactg taatccagag ccattaaatg
     2761 actaaaatga tcgttataaa aaccacggct aaccataaat cgccacattt tctgctttgc
     2821 tttcagatcc catacaagcg gtcgtttttt ctcaaaaatt tgctcagcta attcaaacca
     2881 atccacttcg gcgttttcta attcttggtt aattaaccaa tcttttaccc ctttaagttt
     2941 tagctcttgc tttaaacggc gaggcccaat gccttgcatt gaacgataac ggataaaagt
     3001 ggtacaaaag cgttcatcac tttgccactg attcgcttga gctttttcaa tcgctgcatc
     3061 aatctccgtg agttcatact ctttttgctt tagtttttgc cgaagctctt gctcgctgta
     3121 atcacgcttt gaaagtaaat atagcagata attaaccgct gtgtatttat ttgttttagt
     3181 agtagatgtt gtcatttatc ggttataaat aaaaatgcga ttttcacgtt gagcgaaaac
     3241 cgcataaatt taattagagt tcttcttcac taaattcatc atctgcattg tctgcatttt
     3301 cagaatctgg agtagccgta aaagttgggt ttgaaatgag tagatttcga atatcttgct
     3361 caattttact cgccacttca gggtgttctt ttaaccattt gatggcatta tttttaccct
     3421 gaccgatttt ctccccttca taagagaacc aagcccctgc tttattaatg aagccgtgag
     3481 attccgctaa aatgagtaat tcattcatac gggcaatgcc ctcaccgtac ataatatcaa
     3541 attggacttc acggaacggt ggagcaacct tgtttttcac aattttcact tttgtttcgc
     3601 taccgataac ctcatcaccg tcttttacta cgcctgaacg acgaatatct aaacgtactg
     3661 aagcatagaa tttaagggca ttaccaccag ttgtggtttc agggttaccg aacattaccc
     3721 caattttcat acgaatttgg ttaatgaaaa tcaccaagca gttggtcgct ttgatatttg
     3781 cggttaattt acgcaatgct tgtgacatta aacgagcttg taagcccata tgggaatcac
     3841 ccatatcgcc ttcaatctct gcttttggtg taagtgccgc aacagagtcc acaataatga
     3901 catcaaccgc accggaacga actaacgcat cacaaatttc taatgcttgt tcgccattat
     3961 ccggttgtga aattaataat ccatctgtat ctacccctaa cttacgggcg taaataggat
     4021 caagtgcgtg ttcggcatca ataaaggcac aagttttacc atttttttgg gcttgagcca
     4081 ccacagataa agttaatgta gttttacctg aagactccgg tccataaatt tccacaatac
     4141 gacccattgg taaaccacca atgcctaatg ctgagtctaa accaagagaa ccggttgaaa
     4201 ctgcttcaat atcaagggct tgagtatcgc ctaacttcat aattgagcct ttgccaaatt
     4261 gcttttcaat ttgagctaat gcggctgcca acgctttttc tttttgttcc ggatcaatct
     4321 gtttaaccgg agtgttcttt tgactttttt tctctgccat attctatcct ttgtaaactt
     4381 ttatctaaaa gcaaccgctt gttgctgcaa aataactgta tattattata gtatttactt
     4441 atccagttgc aagcattttt ttaggatttt cgatcaagat cacaaaaata aatttgcgag
     4501 caaggcaatg cccttggttc ctcaaaggga ttaagagcat taatcaacat aattttttca
     4561 tattgatgca aatcttgctt ggctattttg gctaaaacga ctttcccttc atcaagcaat
     4621 ttactaagct gagtgccttt gagcaaataa tgttgcgggc tataccagat tccttgtttt
     4681 aggaacagta aattgccgat ggagcaatca ctgacgtagc cattattgag aataatcacc
     4741 tcttcatttt gtaaaatagg gagcaaatca aaccgcttgc ggtcgctaaa tttgaggtga
     4801 taatcccaat tttccacctc aaccaagcga aaacgttcta cttttttggg cgaatagata
     4861 aaaaatttca gctcaaattc tgttgcgttg taatctattc ggcaacgtac tttgccccgt
     4921 tgagaagcct ctggcacaac taaaatatca gcaaggctaa aacgaggttc acagccaaaa
     4981 tattcttgtg ttgcttgctc aaaacgttgt tgatgatact gtaagttctg aactttgccg
     5041 tcaataatcg aaagggtttc aaatagcgga aatcggcaca tagactttct ctaacagttc
     5101 attatattca tcagttaaca cactttgtga ggtaatgcca ccaccactat gaaacacaag
     5161 cccgtttgag gtttgagcaa tgtaacgaat tgccaccgca ctttctaagc tctcgccatc
     5221 aaaataacca aaaataccgg tgtagtaacc ccgttttcct aattctgctt gctgaataat
     5281 cgaaaccgtt ttctctttcg gtgcaccgct aatggagccg gcaggtagta attttgccaa
     5341 tatcgaaccg atattttctt gccaattttc acctaattcg ccacagatct cggaacttgt
     5401 ctgcaaaatt gcccctcgat gggtttctac tttttccaca taacggtatt tagtcacttc
     5461 aatatttttc gccaccaacg ccaagtcatt tcggatcaaa tccacaatgg tattatgttc
     5521 ggtaaactct ttttccgaag ccattaattt ctgctcggca ttctcctcat ttgcattaat
     5581 tgtgcctttc atcgggtagg aaaaaatgcg gttattttca attcgcacaa agcattcagg
     5641 ggaaaaacag acgaacctgc cttgcaaata acatttatat tttgctttgc tggcggcaaa
     5701 aatttccgcc aatgaataat tggtttcaat ggcagtcggg tagctcaaat tcagcaagta
     5761 gctattgccg gcttgaattt cctttttcac taattcaaac ccggtttggt attcgctaaa
     5821 cggaatcgga tttgccgata attcaaaagc tttagtcggt tttttaaccg gcttttcgag
     5881 attcgatttg caaaaaaatt caaaaaaaag accgcttgcg tgagcctgtt ctagcggaaa
     5941 aataagtggc tttttctgct caaaatcaat taaaaagaaa aaagggcgtt ttgcttgccc
     6001 gtagtgattg gcttggtgaa taaaatcttg catagaaaac acaatggctg aaattggctg
     6061 aatcatcaaa agctctgtta taatgcaaag caatagattt gaaaagcatt ttttaaaaaa
     6121 ggttttaccg atgaacaaca ccattcattt tcaagaaatt gggatctaca tcgcccttgc
     6181 cgtttgtgtg ctggtaggcg tttatctctt tttcttgaaa tcccgttatc agcgagatgc
     6241 ctacgaatta agccaagatc ttggcaaaat caccgaaaat tttgaacaaa ttcagcaaaa
     6301 atatgaggtg ttggctcaag aaaaaaatca gcttgagcag tgggcaattc aacaacagac
     6361 caaatatgaa gcagtgagtg aacgtttaaa cgagcgagat gggcaattgc aacgattcca
     6421 acaaaaaatc gaacttgccg aacagcaaga aaaccagctg gaacgctata ttaatgagct
     6481 aaaagaacgt gtgggttctt cccaagcaaa ggcagaaagt ttggaagaac agctacaatt
     6541 tagccaaagt tcactctcaa ctaaagagcg ggaaaatcaa gcgttattta gccgtttaaa
     6601 cgaggtacaa aacgagctga ccgaactgcg tactacgctt tcggaaaaac aggcaaattt
     6661 tgaagcacaa caacgcaatt ttattgaggt aaaacaacag cttaatgtgg aatttcaaca
     6721 tttggcacaa caaattcttg acgaaaaaag caagcgtttt agcgaaacca atcaaagctc
     6781 gttagaagct ctactgaagc cgttcaaaga gcaaattgaa ggctttcaaa aacgggtgaa
     6841 tgaggtacat agtgagtcgc tcaaaggctc ggcaaattta gaggctgaaa ttaagcgggt
     6901 gctacaaatt ggcgtttcaa tgtcggaaga ggcacaaaat ctggcaacgg cattaaaagg
     6961 caataataaa attgccggca actggggcga agtacagctt gaaagtgcct tacaatctgc
     7021 cggtttactc gcaggcgagc attaccaagc ccaagagagt tttcgtgatg aagaggggcg
     7081 acgttttgcc cccgattttg tggtgcattt gccggatcaa aaacatctga ttattgacag
     7141 taaagtctcg ttagtagcgt acgatcaagc ggtcagatca gaagaaaatt ttgcaatttc
     7201 tcaagcctta gacgaacatt gccgttcgct acgcaatcat attgagggat tgtctaagaa
     7261 gaactacagt gcattattag gcattaaaag cccggatttt gtactgatgt ttattccgat
     7321 agaaccggct tatattgaag cgatgaagca cgatccgcaa ctgtttaact ttggctatga
     7381 acgcaatgtg attttggttt ctcacaccac cttaatgccg attttacgca cggtcgccaa
     7441 tttatggcgg attgaacgag gcaacagcga ggctcgtgaa atcagcgaaa aagccggcga
     7501 aatttacaac caagtgtgtg cggttgccga tcggttagct aaattaggta acacgctaaa
     7561 taccgcaaat aatcaataca atcaaaccgt tacctcgtta gtgggacggc aaggcttact
     7621 gggcaaagtg gaacgattcc aacatctttc aagcaaagca agccaaattg tgccgcaggt
     7681 tgagctgatt gaaaatgagg tggatactat taaattggaa atggtatcga gagaattatt
     7741 ggaagaatag tcaaatctct ttattcccta aaatt
//
LOCUS       DANJWC010000118         7773 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.118, whole genome shotgun sequence.
ACCESSION   DANJWC010000118 DANJWC010000000
VERSION     DANJWC010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7773
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.118"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            487..1005
                     /locus_tag="PWX02_001807"
     CDS             487..1005
                     /locus_tag="PWX02_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein"
                     /protein_id="HDL1729798.1"
                     /translation="MQKKLFIAQIKQNLSELNVFSTDNIFLNSPYFSQQTGLVSVFIA
                     EIEKTVELLLNQTEVLYSEFYAEKLVKQFDALKNAVEKIQSKPESAQFHSSYQFSPNI
                     HRLAPNKRLQEYRKALRALNEKISWLVEQNLNTQNEATKQTLQNQITETEYRKMKCLK
                     AIENLEQELLFK"
     gene            complement(1009..1082)
                     /locus_tag="PWX02_001808"
     tRNA            complement(1009..1082)
                     /locus_tag="PWX02_001808"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1048..1050),aa:Cys,seq:gca)
     gene            complement(1106..1181)
                     /locus_tag="PWX02_001809"
     tRNA            complement(1106..1181)
                     /locus_tag="PWX02_001809"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1146..1148),aa:Gly,seq:gcc)
     gene            complement(1391..2815)
                     /gene="lpdA"
                     /locus_tag="PWX02_001810"
     CDS             complement(1391..2815)
                     /gene="lpdA"
                     /locus_tag="PWX02_001810"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011272714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDL1729799.1"
                     /translation="MSKEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYSTLGGI
                     CLNVGCIPSKALLHVAKVIEEAKNAVHNGVTFGEPTIDLDQVRAGKEAVVSKLTGGLA
                     GMAKARKVTVVEGLAAFTGPNTLVARDRDGNPTTITFDNAIIAAGSRPIQLPFIPHED
                     PRVWDSTDALKLKEVPKKLLIMGGGIIGLEMGTVYNALGSEVEVVEMFDQVIPAADKD
                     VVAIYTKQIEKKFKLMLETKVTAVEAKDDGIYVSMEGKACNDTKRYDAVLVAIGRVPN
                     GKLIDAGKAGVEVDERGFIRVDKQMRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGMKHYFDPKVIPSIAYTEPEVAWVGKTEKECKQEGLNYEVAKFPWAASGRAIASEC
                     SEGMTKLIFDKDTHRVLGGAIVGSNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNAKAKKC"
     gene            complement(3015..4919)
                     /gene="aceF"
                     /locus_tag="PWX02_001811"
     CDS             complement(3015..4919)
                     /gene="aceF"
                     /locus_tag="PWX02_001811"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDL1729800.1"
                     /translation="MSKQINVPDIGSDEVSVTEVMVKVGDTISVDQSIINVEGDKASM
                     EVPAPEAGVVKEVLVKVGDKVSTGSPMLILESADAAPAQEAPKAEAAPAAPVATTSAV
                     VEVNVPDIGGDEVNVTEIMVKVGDSVEVDQSIINVEGDKASMEVPAPVAGVVKEILIN
                     VGDKVSTGKLIMKFETASAATAQSAPVAEAAPAQASASAIKDVNVPDIGGDEVNVTEI
                     MVKVGDSVSEEQSLITVEGDKASMEVPAPFAGVVKEILVKSGDKVSTGSLIMKFEVAG
                     AAPAPAAAQAASAPAPQAAAPAKADAAPAGQNQSGLSQEQVVASAGYAHATPVIRRLA
                     REFGVNLDKVKGTGRKGRIVKEDIQAYVKTAVQVFEKQGGTAAAATGAANGAGLGLLP
                     WPKVDFSKFGEVEEVELSRINKISGANLHRNWVMIPHVTHFDRTDITELENFRKEQNK
                     EAEKRKLDVKITPVVFIMKAVASALEAFPRFNSSISEDAQRLTLKKYINIGIAVDTPN
                     GLVVPVFKNVNKKGIIELSRELMEVSKKAREGKLTASDMQGGCFTISSLGGIGTTHFT
                     PIVNAPEVAILGVSKSEMQPIWNGKEFEPRLMLPLSLSFDHRVIDGADGARFLSYINS
                     VLADIRRLVM"
     gene            complement(5123..7747)
                     /gene="aceE"
                     /locus_tag="PWX02_001812"
     CDS             complement(5123..7747)
                     /gene="aceE"
                     /locus_tag="PWX02_001812"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005550031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HDL1729801.1"
                     /translation="METQDWLASLDSLIREEGLERAQFIIEQVISQARAGGVALPTGV
                     TTDYVNTIPVSEQPAYPGDHKIERRIRSAVRWNAIAAVLRSQKKDLDLGGHISTFQSA
                     ATMYEVCFNHFFKAPTEKDGGDLVFFQGHAAPGMYGRAFLEGRITAEQMDNFRQEAFT
                     DGLSSYPHPKLMPEFWQFSTVSMGLGPVNAIYQARFLKYLENRGLKDTSAQKVYAFLG
                     DGEMDEIESKGALSFAGREKLNNLIFTISCNLQRLDGPVNGNGKIVQELEALFTGCGW
                     EVIKVLWGSGWDKLLEKDTSGKLKQLMMEVLDGDYLTFKSKNGAYVREHFFGRYPETA
                     ALVADMTDDEIWALRRGAHDSEKLYAAYAKAQKSDKPVVILAHQVKGYRIPEAESKNT
                     AHQSKKMSLDSLKGFRDYFELPLTDEQVESLEYVRFPEGSEEYNYLHGQRKALNGYVP
                     VRKPKFTVDFKVPELSEFQALLDEQPRGISTTMAFSRVLNTLLKDKNIGQQIVPIIAD
                     EARTFGMEGLFRQIGIYNPHGQNYVPSDRDLVAYYREAKDGQVLQEGINELGATASWV
                     AAATSYSVSNLPMIPFFIYYSMFGFQRVGDMMWLAGDQLARGFMIGGTSGRTTLNGEG
                     LQHEDGHSHIQAGVIPNCVTYDPAFAFEVAVIVQDGIRRMYGENQEDIFYYITTLNEI
                     TEQPAMPAGAEEGIRKGLYKFETVEGKGKGHVQLLSSGAIMRHVRAAAQILANDYGIT
                     ADVFSAPSFNELGRDGADVARWNLLHPTETPRVPYVAQVLQDLPTVASTDYMKMYAEQ
                     IRAYVPSKHYHVLGTDGFGRSDSRANLREHFEVDERYVVVAALAQLAKEGTVENKVVA
                     EAITKFGLNVDRINPLYA"
BASE COUNT         2245 a         1727 c         1428 g         2373 t
ORIGIN      
        1 tttatatgtt atatgataag taattaccct cattaaggtg taagagtaat tatccaagat
       61 acaatgtatc agcaaaacgc ctaacattgt tgtcgccata ataattgcaa aaagaaaact
      121 caatgtcaat agttcaaact ccaaaacaaa gacggagttt ttcttttaaa atcaaatggt
      181 tataattaca ttatttcatt tggagaaaat ccactcattt ttaacagaaa aaatgaagcg
      241 cataacatat tgataaataa ggatttttca tatttaattt gatatagttc aaacttatca
      301 aaaagttcat ttattttgtg acatagttca catttttgat atttaatttc ttttttgcct
      361 gtttttcacg cacgaattgt aagaaattag taaaaatcaa ccgcttgtac gcaaagtgtt
      421 ttgctttaaa atagaagtta tatgaaataa aattcactta aattttcttt attgataaca
      481 tcaaccatgc aaaaaaaact ttttatcgcc caaataaaac aaaacctctc ggaattaaat
      541 gtattttcaa cggataatat ttttctgaat tcaccctatt tctcacagca aaccggtttg
      601 gtgagtgtgt tcattgctga aattgaaaaa acagttgagc tattattgaa ccaaactgag
      661 gtgctatata gtgaatttta cgctgaaaaa ttggttaaac aatttgatgc attaaagaat
      721 gccgtagaaa aaatccaaag taagccagaa tcagctcaat tccattcttc ctatcaattt
      781 tctccaaata ttcatcgttt agcgcccaac aaacgcttac aagaataccg taaagcgtta
      841 cgtgcattaa atgagaaaat tagctggcta gttgaacaaa atttaaatac acaaaatgag
      901 gcaactaaac aaaccctgca aaaccaaatc acagagacag aatatcgcaa aatgaaatgc
      961 ttaaaggcga ttgaaaattt agaacaagag ttgctattta aatagtgatg gaggcgtgtc
     1021 ccggagtcga accgagctac atggatttgc aatccagtgc ataaccgctt tgctaacacg
     1081 ccataagatt tgaagatttg gaaattggag cgggaaacga ggctcgaact cgcgaccccg
     1141 accttggcaa ggtcgtgctc taccaactga gctattcccg catttgaagt ctcacaacat
     1201 tgcgctgtgg aacgtggtgc attttacgga tttatcattt tatgtcaatg ctaaatttag
     1261 cattatagat tcaaatgctg aaaaaaaact cactattcaa tagtttagtt ttaaaaataa
     1321 tcaagcggtt gtattttgca aaaaatttac aaaatacaac cgcttatttt ttagagatta
     1381 aataatctga ttaacacttt ttggctttcg catttggtaa gtctgtaatt gaaccttcaa
     1441 atacttctgc cgctaatcct accgattcgt gtaacgttgg gtgagcatga atggttaatg
     1501 cgatatcttc agcatcacag cccatttcga ttgcaagacc gatttcgcct aataattcgc
     1561 caccattgct accaacaatc gcaccaccta atacacggtg agtatcttta tcaaaaatta
     1621 acttggtcat accttctgag cattcagaag caatcgcacg acctgaagca gcccaaggga
     1681 atttcgccac ttcgtagttc aagccttctt gtttacactc tttctcagtt ttaccaaccc
     1741 aagccacttc tggttcagta taagcgatgg acggaatgac tttcggatcg aagtaatgtt
     1801 tcattcctgc aatcacttct gctgccacgt gtccctcgtg tacacctttg tgagctaaca
     1861 tcggctggcc gacaatatca ccaatagcaa agatgtgtgg cacattggta cgcatttgtt
     1921 tatctacacg aataaagcca cgctcatcta cttccactcc agctttaccg gcatcaatta
     1981 atttaccgtt tggcacacgt ccgatagcga ctaataccgc atcataacgt ttagtatcgt
     2041 tgcaagcctt accttccatt gatacataga taccgtcatc tttagcttct actgcggtta
     2101 ctttggtttc aagcattagc ttgaattttt tctcgatttg tttggtgtaa atcgctacaa
     2161 catctttatc tgcagcagga attacttggt cgaacatttc aaccacttcc acttctgaac
     2221 caagcgcgtt atataccgtt cccatttcta aaccgataat accaccaccc ataataagga
     2281 gttttttcgg tacttctttt aatttaagag catcggttga atcccaaact cgtgggtctt
     2341 cgtgtggaat aaatggtagt tgaatcggac gagaacctgc tgcgataatc gcattatcga
     2401 acgtgatagt tgtaggattg ccgtcacgat cacgagcgac taacgtattc ggaccagtaa
     2461 atgcagccaa gccttctact actgtcactt tacgagcctt agccattcct gctaagccac
     2521 cggttaattt tgaaacaacg gcttctttac cagcacgtac ctgatctaaa tcaatcgttg
     2581 gctcaccaaa ggtcacaccg ttgtgtactg catttttagc ttcttcaatt acttttgcaa
     2641 cgtgtaataa tgctttagaa gggatacaac ctacgtttaa gcaaatacca cctaaggttg
     2701 aataacgctc aacaattact gtttctaagc ctaagtcggc acaacggaat gccgctgagt
     2761 aacctgctgg acctgcacca agtacgacaa cttgggtttt aatttctttg ctcatagttt
     2821 ttcctctttt tattcccccc ctttagcaaa gaggggtaag ggagatttgg cagaatataa
     2881 agcgaagtga gagagcaatc aatcaaacac aaatttctac caaataattt attctcttcg
     2941 taaattacgt ctatcaaatc tccccctacc cctcttttct aaagagggac ttttttagga
     3001 ggagctttac cgaattacat cactaaacga cgaatatctg ccaacacgct attaatatag
     3061 cttaagaatc tcgcaccatc agcaccatcg atcacacggt ggtcgaatga taatgaaagc
     3121 ggaagcatta agcgtggttc aaactctttg ccattccaaa tcggttgcat ttcagattta
     3181 gacacaccta aaatagccac ttccggtgca ttcacgattg gcgtaaagtg cgttgtacca
     3241 ataccgccta aacttgaaat tgtgaaacag ccaccttgca tatcagatgc tgtcaattta
     3301 ccttcacggg cttttttcga gacttccatt aattcgcgag aaagttcgat aatgcctttt
     3361 ttgttcacat ttttgaacac cggcaccact aaaccgtttg gcgtatcaac cgcaatcccg
     3421 atattgatgt attttttcaa ggttaaacgt tgtgcatctt cagaaattga gctgttgaaa
     3481 cgagggaagg cttctaatgc acttgccact gctttcataa tgaagaccac aggggtaatt
     3541 ttcacgtcta atttacgttt ttccgcttct ttattttgct ctttacggaa gttttctaac
     3601 tcggtaatgt ctgtgcgatc gaaatgtgta acgtgaggaa tcattaccca gttacggtgt
     3661 aagttcgcac cggaaatctt attgatacgg cttaattcaa cttcttcaac ttcaccaaac
     3721 ttgctgaaat ctactttcgg ccacggtaat aaacctaagc ctgcaccgtt tgccgcacct
     3781 gttgctgccg ctgctgttcc gccttgtttt tcaaatactt gaacagccgt tttaacgtat
     3841 gcttgaatat cttctttaac gatacggcct ttacgacctg taccttttac tttgtctagg
     3901 tttacaccaa actcacgagc taaacggcga attaccggtg ttgcgtgtgc atagccagct
     3961 gaagccacca cttgttcttg gcttaaacct gattggtttt gacctgccgg agctgcgtca
     4021 gctttcgccg gagctgctgc ttgtggagca ggagctgaag ccgcttgagc agccgccgga
     4081 gccggtgccg cacccgctac ttcaaatttc ataattaatg aaccggttga aactttatca
     4141 cctgatttca ctaaaatttc tttcaccaca ccggcaaatg gagccggaac ttccattgaa
     4201 gctttatcac cttcaaccgt gattaaagat tgttcttctg aaacagaatc acccacttta
     4261 accataattt cagttacgtt tacttcatca ccaccgatgt ccggtacatt tacatcttta
     4321 atcgctgatg ccgaagcttg agctggagca gcttccgcaa ccggtgcaga ttgtgctgta
     4381 gctgcggaag ccgtttcaaa tttcataatc aatttaccag ttgaaacttt gtcacccaca
     4441 ttgattaaga tttcttttac tacgccggca accggtgcag gcacttccat tgaggcttta
     4501 tcaccttcaa cgttgatgat agattgatcc acttcaacgc tatcacctac tttaaccata
     4561 atttcggtta cgttaacttc atcgccaccg atatcaggta catttacttc aacaacggct
     4621 gaagttgttg ctactggagc agcaggagct gcttctgctt ttggtgcttc ttgagcagga
     4681 gcagcatctg ctgactctaa aattaacatt ggtgaaccgg ttgaaacttt atcacccact
     4741 ttcactaaaa cttcttttac cacacctgct tcaggagccg gcacttccat agaggcttta
     4801 tcgccttcta cgttaataat tgattgatct actgaaatag tatcaccaac ttttaccatc
     4861 acttcagtca cactgacttc atccgaaccg atgtccggca cattaatttg ttttgacatt
     4921 ttgaaatcct tcttgagtta ccccctctcc cattgtggga aggacagatt tttctttgta
     4981 agaagaaaaa tcagggagag gggaatgcaa gcggttgatt ttctcaacat ttttgcaaat
     5041 aaaactaata ccctctctgc taaaagtttc taaacgaaac ctttagctgt ctctctccca
     5101 tcaagggaga gagtattaga aattacgcat ataatgggtt aatacgatct acatttaagc
     5161 cgaatttagt aatagcttcc gcaaccactt tgttttcaac tgtaccttct ttcgctaatt
     5221 gagcgagggc tgcaactact acataacgct catcaacttc gaagtgttca cgtaagtttg
     5281 cacggctgtc tgaacgaccg aaaccgtctg tacctaacac gtgatagtgt ttacttggta
     5341 cataagcacg gatttgctct gcgtacattt tcatatagtc ggttgaagca acagttggta
     5401 aatcttgtaa cacttgagca acgtacggta cacgtggtgt ttcagttggg tgtaataagt
     5461 tccaacgagc cacatctgct ccatcacgac ctaattcatt gaatgatggt gctgagaata
     5521 catctgctgt aatgccgtag tcattcgcga gaatttgtgc agctgcacgc acgtgacgca
     5581 taattgcacc tgagcttaat aattgtacgt gacctttacc tttaccctca acagtttcaa
     5641 atttatataa acctttacgg ataccttctt ccgcacctgc cggcattgcc ggctgttcgg
     5701 taatttcatt taaggtagta atgtagtaga agatatcttc ttggttttca ccatacatac
     5761 gacgaatacc gtcttgaaca ataaccgcaa cctcaaaggc aaatgcaggg tcataagtga
     5821 cgcagttcgg gattacacct gcttgaatat ggctatgacc atcttcgtgt tgtaaacctt
     5881 caccgtttaa tgttgtacga cctgaagtac caccaatcat aaagccacga gctaattggt
     5941 caccggctaa ccacatcata tcgcctacac gttggaaacc aaacattgag tagtagatga
     6001 agaatgggat catcggtaag ttgctaacag aataagaagt cgctgctgcc acccaagatg
     6061 ctgttgcacc taactcattg ataccttctt gtaacacttg gccatctttt gcttcacggt
     6121 agtaagctac taaatcacgg tctgacggta catagttttg accatgtggg ttataaatac
     6181 cgatttgacg gaataaacct tccataccga aagtacgggc ttcgtccgca ataataggta
     6241 cgatttgctg accgatattt ttatctttca ataaagtatt caatacacga gagaacgcca
     6301 ttgtggttga aataccacgt ggttgctcat ccaataatgc ttggaattcg cttaattcag
     6361 gcactttgaa atcaaccgta aatttcggtt tacgtacagg cacataaccg tttaaggctt
     6421 tacgttgtcc gtgtaagtag ttgtactctt cagaaccttc agggaatctt acatactcta
     6481 ggctttctac ttgctcatcg gttaatggta gctcgaagta gtcacggaag ccttttaagc
     6541 tatctaatga catttttttc gattggtgag cagtattttt actttccgct tcaggaatac
     6601 ggtagccttt cacttggtga gctaaaatta caaccggttt gtccgatttt tgtgcttttg
     6661 cataggctgc ataaagtttt tcagaatcgt gtgcaccacg gcgtaatgcc caaatttcat
     6721 cgtctgtcat atcagcaact aatgctgcag tttcagggta acgaccgaag aagtgttcac
     6781 gaacgtaagc gccatttttg gatttgaagg ttaaatagtc accatcaagc acttccatca
     6841 ttaactgttt aagtttgcct gaagtatctt tttcaagcag tttatcccaa ccactacccc
     6901 ataacacttt aatcacttcc cagccacaac cggtaaagag tgcttctaat tcttgcacga
     6961 ttttgccgtt accgttaacc ggaccgtcta aacgttgtaa gttacagcta atagtgaaga
     7021 ttaagttatt taatttttca cgaccggcga atgataatgc acctttagat tcgatttcat
     7081 ccatttcgcc atcgcctaag aatgcatata ctttttgagc cgaagtatct tttaggccac
     7141 ggttttctaa atattttaag aaacgagctt ggtaaatagc atttaccggt cctaaaccca
     7201 tagatacggt agagaattgc caaaattcag gcattaattt agggtgtggg taagaagata
     7261 aaccgtctgt aaatgcttct tgacggaagt tatccatttg ttctgcggta atacgacctt
     7321 ctaagaacgc acgaccatac atacccggtg ctgcgtggcc ttggaagaac actaaatcac
     7381 caccatcttt ttcggttggt gctttgaaaa agtggttaaa acacacttca tacatagttg
     7441 ccgcagattg gaaagttgaa atatgaccac ctaagtcaag atctttcttc tggctacgaa
     7501 gtactgccgc aatcgcattc caacgtaccg cagaacgaat acgacgttcg attttatgat
     7561 cacccggata agctggctgc tcggaaacag ggatagtatt gacataatct gttgttacgc
     7621 ctgttggtaa tgccacgcca cctgcacgag cttggctaat aacttgttca atgataaatt
     7681 gagcgcgctc taaaccttct tcacgaatta atgaatcgag tgatgctaac caatcctgag
     7741 tttcgattgg atctacgtca tttcttaagt tct
//
LOCUS       DANJWC010000119         7629 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.119, whole genome shotgun sequence.
ACCESSION   DANJWC010000119 DANJWC010000000
VERSION     DANJWC010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7629
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.119"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..698)
                     /locus_tag="PWX02_001813"
     CDS             complement(<1..698)
                     /locus_tag="PWX02_001813"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729802.1"
                     /translation="MENLKLLTHPWAEKGLKNEIPLNREGSGVTSATATYEDGFPDVT
                     MKPIHEGGKAPSGKDMNGVLYELSTHIVHLNKGCLYPFEMDFCNAIGGYPKGSLLISN
                     DKGKLFISLIDNNTTDFNSENYFGKWDCISTQDLLNSPDGFKFIGQCESIAQLRTIEP
                     TEDQQRILVKSYYAGKNLGGGEFYADFADTTTADNAGTVIVTAGGKRWKRVYSTLNIF
                     DFGVSLDSVNNDAI"
     gene            complement(771..1391)
                     /locus_tag="PWX02_001814"
     CDS             complement(771..1391)
                     /locus_tag="PWX02_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HDL1729803.1"
                     /translation="MNKPHLQKTIISQYANSPIMNRIIQFADEYLETKDCFRQFYNIY
                     WDIDTAEGVGLDFWGVVLGLSRFVEINDQTTFQGSSIASTELKDKVNPTSYRMNDAMY
                     KSMLFVKAMTNIVYATAPNINKILQILFKERGRAYFVKSGTMTARYVFEFKLLDVEKA
                     IVMTLLPRPTGVLCDFYEPEQLYTWGFNENELAPFGQAAFYIGDKN"
     gene            complement(1388..2584)
                     /locus_tag="PWX02_001815"
     CDS             complement(1388..2584)
                     /locus_tag="PWX02_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDL1729804.1"
                     /translation="MTIQTNVPSIQFTPQGLILPSEQEILNGVLADFNAAFGGGLSQN
                     LETPQGQLASSIAAIIADKNNQIAWLVNNLDPTYSDGIMQDAIAKIYFVKRKGRVNSF
                     ATCQFVGLPGVTIPKGLVIKDTTNNEWILDEEISILENGTVEGRVIANGVYGAKANTI
                     TKIHQSIIGLDRVTNPQDAVVGTERESRQDFAERYRESVAMNAQGMPSAVHANVMKLE
                     GVIDCYVIDNPTNKTVKHGATGYPIKPHSVYVAVRGGNDLDIAKMIWLYSGNGCDFNG
                     NTEIVVTDDTYVTPKPTYYINFMRPLDTPIFFRVKIKSGLVGDYLNEMKEAIKMQFDQ
                     SSHRKIGRIFYAMNYVSSIIKSFPKDYLLDVEVSSDGVNFENKVQLGIDQYPILFSEN
                     IILVEE"
     gene            complement(2581..2931)
                     /locus_tag="PWX02_001816"
     CDS             complement(2581..2931)
                     /locus_tag="PWX02_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729805.1"
                     /translation="MTTLFLHPQRWDLCLDDEGNIAYAKDPYAKAQDVASAIKLFKGE
                     LYYDTEKGIPYFDETLGKKQSLALYQYRLEQAALRVKGVVSAKANIESGASREVTGSV
                     TFTDEQNRQMSVKL"
     gene            complement(2935..3597)
                     /locus_tag="PWX02_001817"
     CDS             complement(2935..3597)
                     /locus_tag="PWX02_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HDL1729806.1"
                     /translation="MSNNLGLLKPDQVLGGTGEFEFIISGIVNRIQTVTLVQVIAVNA
                     TGIGPVGTVDVQPMVAQLDGSGKAYAHGVIHNIPYFRLQGGSNAVIIDPEVGDIGMCG
                     FCSRDISSVKVNKAPSTPQSRRKFDYADGLYFGGFLNGVPSQYLFFKDSGIELVSTGE
                     ILLKGTSIKLDAPVETTSTVNAAGDITDNAKSGGKSMKNMRQVYDTHTHNGGPQPDRK
                     TG"
     gene            complement(3587..4474)
                     /locus_tag="PWX02_001818"
     CDS             complement(3587..4474)
                     /locus_tag="PWX02_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729807.1"
                     /translation="MSSFTKKKLKFSFVLGQQDPHFDKDENNTVIVKGLRASVTIQSG
                     HGALMPSAKLMIYGLGQKVMNKLAKIRWNTDDAKLNYVRVEASQDGGESYILVYEGVI
                     QYAYPNYGSSPEVILTIDSTSALQHKIRPVPPLSFNGEVDVASAAAQICQKMSMQFEN
                     NGVTEKVSNPYLSETALDQVFKLCDAINAKAVVEFNTIAITPQGQARDILVPVISPSS
                     GLIGYPTPTITGTRFQCLFDPVIRVQGLVEIKDSLVEICNGKWLVVGLSHYLESEVSG
                     GRWLSEVDAIYPGGVKIVK"
     gene            complement(4474..4770)
                     /locus_tag="PWX02_001819"
     CDS             complement(4474..4770)
                     /locus_tag="PWX02_001819"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729808.1"
                     /translation="MDLYHIPLKAVANQRVRCQIEGQELEITLHTRLEEQLYISIKCN
                     GVAVVHNRICQDLTPLIDVGYLPMKGVLFFKDMQGRQAPHCKELGKRYRLFWGA"
     gene            complement(4773..5480)
                     /locus_tag="PWX02_001820"
     CDS             complement(4773..5480)
                     /locus_tag="PWX02_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729809.1"
                     /translation="MTWSSVGIPSVPGLPKNIGDAAIKFGGALGINLLFGNYWGVFDQ
                     NGIPLLLADNVKSVKYQSRSKISTAPLEKGSFAAYNKVIEPYTVNVVMTKGSGGVKER
                     GAFLALVDAFANSTDLFMVITPEAIYPNCNIVGYDYVREAGDGARLIKVNLHLQEIRE
                     VEVEYTKTKAESAEAVQEQGNVTPEKVNTEAGNTSVLFDIGQEIGDMKETLKTEGAGA
                     VIDKLKDGLMNAVGMGG"
     gene            complement(5715..6611)
                     /locus_tag="PWX02_001821"
     CDS             complement(5715..6611)
                     /locus_tag="PWX02_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HDL1729810.1"
                     /translation="MSNQITTQTLSFYGSDLITLKVEDVIYTAVRPIVDALGLSWGSQ
                     QRKLENSKEKFSCTHMKTTGSDGKTYEMLCMPIKKLNGWLFSINPEKVRVDLKEKVIC
                     YQEECFEALYNYWHLGKAERLQNSAENQTALPEPTLTAEEQNMLQNAVKATHERTKLS
                     YGEIWARTKNKFRVAEYKQIKCSDLRDALIYVASMQPACIDRPRQTINVDWFNMMNFA
                     LLLKQYHRFTKDLPHIHTYLSALPEHSAMAGLLKNHLTEMKNNDRLFTDSILSLSEIS
                     NHFLPQDQWKSLMRIFSEMIVE"
     gene            complement(6896..7075)
                     /locus_tag="PWX02_001822"
     CDS             complement(6896..7075)
                     /locus_tag="PWX02_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729811.1"
                     /translation="MSNYSKTKLKIHLKKGLEMETNASPIMRGAIAISIVLIALGVFA
                     LCVTPLANVLIEMIK"
     gene            complement(7227..>7629)
                     /locus_tag="PWX02_001823"
     CDS             complement(7227..>7629)
                     /locus_tag="PWX02_001823"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729812.1"
                     /translation="IKALLALFVIFLILDFIAWSSGNKTASEVKAKQLISDKQCNQLI
                     KDLIYHKHAQDICLVEAKLGEFNSNLNGDSFAEYSTRNGCPQLPENVAYKLQEETSLN
                     FINALKSSPSKVEFCKKELPYFKKLVQKYAS"
BASE COUNT         2123 a         1675 c         1371 g         2460 t
ORIGIN      
        1 ttgatggcat cgttattcac gctgtctaag ctgactccaa agtcaaagat attgagtgtt
       61 gaataaactc gtttccatcg tttgccacca gccgtcacaa tcaccgtgcc ggcattgtct
      121 gcggttgtgg tatccgcaaa atcagcataa aactctccac cgcctaaatt tttcccagcg
      181 tagtaggatt taaccaaaat gcgttgttgg tcttctgttg gctcgatggt gcgtaattga
      241 gcaatagatt cacattgacc gatgaactta aatccgtcag gagaattaag taaatcctga
      301 gtagaaatgc aatcccactt cccgaagtaa ttttcggaat tgaaatcagt tgtattgttg
      361 tctatcaagc taataaatag ctttccttta tcattgctaa ttaatagtga accttttgga
      421 tatcctccta tagcattaca aaagtccatt tcaaatggat aaaggcaacc tttattaagg
      481 tggacaatat gtgtcgataa ctcatataac actccgttca tatccttacc cgaaggtgct
      541 tttcctcctt catgaatagg cttcatagtt acatccggga aaccatcttc gtaagtcgcc
      601 gtggcggatg taactcctga accttctcga ttcagtggta tttcattttt taatcccttt
      661 tcagcccaag gatgcgtgag tagctttaag ttttccatga tttctccaac aaaaaaccgt
      721 ttatggaata agaaccataa acggttaagt taatgaataa tttaatttgt ttagttttta
      781 tcgccaatat aaaaagcagc ttgcccgaat ggagctaact cattttcatt aaatccccaa
      841 gtatataatt gctctggctc ataaaagtca caaagcacac cggttggacg aggtagtaat
      901 gtcataacta ttgccttttc tacatccagc agtttgaatt caaaaacata cctagccgtc
      961 attgttcccg acttaacaaa gtaagctcga cctctttctt taaatagtat ctgcaggatc
     1021 ttattgatat tcggtgctgt tgcatacaca atattcgtca ttgctttaac aaaaagcatt
     1081 gacttataca ttgcatcatt catcctgtag ctagttgggt taactttatc ctttagctca
     1141 gtcgaggcta tactggaacc ttgaaaggta gtttggtcat taatttctac aaaacgagat
     1201 aacccaagca ccactcccca aaagtcaagt ccaacacctt cagcagtatc aatgtcccaa
     1261 taaatattat agaactgcct gaagcaatct tttgtctcaa gatattcatc agcaaattga
     1321 atgatacggt tcataattgg actgtttgca tactgactaa tgattgtttt ttgtagatga
     1381 ggtttattca ttcttccacc aaaattatat tttccgaaaa aagaatgggg tattgatcta
     1441 tccccagctg aaccttattc tcaaaattaa caccatcact acttacttca acatcaagca
     1501 agtaatcctt aggaaaagat ttaatgatcg aagaaacgta attcattgca taaaaaattc
     1561 taccaatctt gcgatgagaa gattgatcaa attgcatttt aattgcttct ttcatctcat
     1621 tgagataatc gccaaccagc ccagacttaa tttttaccct aaagaaaatt ggcgtatcca
     1681 aaggtctcat gaaattgatg taatatgttg gcttaggagt aacgtaagta tcatcagtga
     1741 ctactatttc agtattacca ttaaagtcac atccattacc agaatataac catatcattt
     1801 ttgcaatatc taaatcattt cctcctctta cagcaacata aacactatga ggttttatag
     1861 gatatccagt tgctccatgt tttactgttt tgtttgtagg attatcaata acatagcaat
     1921 caatcactcc ttctaatttc attacattag catgcacagc agatggcata ccttgagcat
     1981 tcatagctac gctttctcta tatcgttccg caaaatcctg tctgctttcc cgttctgtac
     2041 caacaaccgc atcttgtggg ttggtaacac ggtctagccc gataatcgat tgatgaattt
     2101 ttgtgatcgt attagctttt gcaccataaa caccgttagc aataacacgt ccttccacag
     2161 tgccgttttc taatatgctg atctcttcat ctaatatcca ttcattattg gtggtatctt
     2221 tgataactaa cccttttgga atagttacac ccggtaaacc aacaaattga caggtggcaa
     2281 aagagttcac tcgccctttt cgttttacga aataaatttt agcaatagca tcttgcataa
     2341 tgccgtcgga ataagtcgga tcaagattat tcactagcca cgcaatttga ttatttttat
     2401 cagcaataat cgcagctatc gagctggcaa gttgtccctg tggtgtttct aagttttggc
     2461 ttagtccacc accaaatgcc gcattaaagt ccgccagcac accgtttaga atttcttgtt
     2521 cgctcggtaa aattaagcct tgcggtgtaa actgaattga tggcacattc gtctgtatcg
     2581 tcatagtttc acgctcatct gtctattttg ttcatcggta aaagtcacag agccggtcac
     2641 ttcccgactg gctccgcttt caatatttgc tttggcagaa accactcctt taactctgag
     2701 ggctgcttgc tctaagcgat attgatataa tgctagagat tgcttcttcc caagtgtttc
     2761 atcaaaataa ggaatccctt tctccgtgtc ataatacaac tcgcctttaa acagctttat
     2821 ggcacttgcc acatcttgag ctttagcata aggatctttt gcataagcga tattcccctc
     2881 atcatcaagg cataaatccc acctttgcgg gtgtaaaaat aacgttgtca tacattaccc
     2941 cgttttccgg tcaggttgtg gcccaccatt gtgcgtgtgc gtatcataaa cctgtcgcat
     3001 atttttcatt gatttacctc ctgatttggc attatcggta atatctccgg cagcgtttac
     3061 tgttgaggta gtttcaacag gggcatctaa tttgattgat gtgcctttta gcaaaatttc
     3121 accggtagaa accaactcaa tgcccgaatc tttgaaaaag agatattggc tcggcacacc
     3181 gttaagaaaa ccgccaaaat acaagccatc tgcataatca aattttcgtc tgctttgtgg
     3241 ggtcgagggg gctttgttca ctttcactga ggaaatatca cgactgcaaa aaccacacat
     3301 tccaatatcg cctacttccg gatcgataat aaccgcattg ctgccaccct gaagtctaaa
     3361 ataagggata ttgtggatta caccgtgtgc atacgctttg ccactaccgt cgagttgtgc
     3421 caccattggt tgcacatcta ccgtgccgac cggtccgatg ccggttgcat ttaccgcaat
     3481 cacttgcact aatgtcactg tttgaattcg attcacaatg ccggaaataa taaattcaaa
     3541 ttcgcctgtg ccacctaaaa cttgatcggg ctttagtaaa cctaaattat ttgacaattt
     3601 tcacacctcc ggggtaaatg gcatcgactt ctgataacca acgccctcct gatacttcac
     3661 tttctaaata atggcttaat ccaacaacca gccatttgcc attacaaatt tcaactaaac
     3721 tgtctttaat ttccacaagc ccttggacgc gtataacagg atcaaataaa cattgaaacc
     3781 ttgtgccggt aatggttggg gttggatatc caattaatcc gctactcggc gaaataacgg
     3841 gaactaaaat atctcttgct tgaccttgcg gcgtgatagc aatcgtatta aattccacga
     3901 ccgcttttgc attgatggca tcacataatt taaaaacttg atctaaagcg gtttcagaca
     3961 agtaagggtt gctcactttc tctgttacac cattattctc aaattgcata ctcatttttt
     4021 ggcaaatttg agcggccgct gaagccacat caacttcacc gttaaaactt aatggcggaa
     4081 ccggtctaat tttgtgttgt aaagctgatg tactatcaat cgttaagatc acctcagggc
     4141 ttgaaccata attcggatag gcatattgaa taacgccctc ataaaccaat atatagcttt
     4201 ctcccccatc ttggcttgcc tctactctga cgtaattcag cttagcgtca tcagtattcc
     4261 agcggatttt cgccaattta ttcattacct tttgaccaag cccgtatatc attagctttg
     4321 cactcggcat tagtgcaccg tgtccgcttt gaattgtgac actcgccctc aatcccttaa
     4381 caatcaccgt attattttca tctttgtcaa aatgcggatc ttgctgtcca agcacaaaag
     4441 aaaacttcag ctttttcttg gtaaaacttg acattacgcc ccccaaaaca agcggtatct
     4501 tttgcctaat tctttgcaat gcggagcttg ccgcccttgc atatctttaa aaaacaaaac
     4561 gcctttcatc ggcaggtaac ctacatcaat taaaggcgtt aaatcttgac aaattcggtt
     4621 atgcaccacc gctacaccgt tgcattttat gctgatataa agctgttctt ccaaacgggt
     4681 atgcaaggtg atttcgagtt cttgtccctc aatttggcag cgtactcgtt gatttgccac
     4741 cgctttaagt ggaatatgat ataagtccaa aatcaacctc ccattccaac ggcgttcatc
     4801 aagccatctt tcagtttatc aataactgca ccggccccct cggtttttaa tgtttctttc
     4861 atatcaccga tctcttgtcc tatatcaaaa aggactgaag tattgccggc ttcagtattt
     4921 actttctctg gcgttacatt cccttgctct tgcacagctt ctgccgattc tgctttagtt
     4981 ttcgtgtatt ccacctctac ctctcgaatt tcctgcaggt gtaaatttac tttaatcaac
     5041 cttgcaccgt ctccggcttc acgcacatag tcataaccaa cgatattgca gttcgggtaa
     5101 atagcttcag gagtgatcac cataaataaa tcagtgctgt tggcaaaggc atcgacaagg
     5161 gctaaaaatg cccctcgttc tttcacaccg ccactgcctt ttgtcattac tacattcacg
     5221 gtataaggct cgatcacttt gttgtaagcg gcaaatgagc ctttttcgag tggtgcggta
     5281 gaaatctttg aacggctctg atatttcacc gatttcacat tatccgcaag taaaagagga
     5341 atgccgtttt gatcgaacac tccccaataa ttgccaaata ataaattgat acccaacgca
     5401 ccaccgaatt ttattgccgc atcgccgata tttttcggta accccggcac actcggtatg
     5461 ccgacagaac tccaagtcat attttctcca aaaaaataac cgcttgcgga acaagcggtt
     5521 gggttaatta acgttgagct gtacaaattt ttccgtcgca gtcattgcct aactctaagt
     5581 agtcagcaga gatagcaaag gcggttaaaa atagaatgat ttttgcgtat ttcatcgtct
     5641 gtttctctga atttgggtgt aagaaacccc tgccgaattt cagtagaaat agcagtcagg
     5701 gggggctttt gggattactc cacaatcatt tcgctaaaaa tccgcattag gcttttccat
     5761 tggtcttgcg gtaggaaatg gttcgagatt tcagagagcg acaaaatact gtcggtgaat
     5821 aaccggtcgt tattcttcat ttctgtcagg tgatttttca gcaatcctgc cattgcactg
     5881 tgctcgggta gtgccgacaa gtaggtgtgg atatgcggta aatctttggt gaaacggtgg
     5941 tattgtttca gtagcaaggc gaagttcatc atattgaacc aatccacatt gattgtctgt
     6001 cttggtctat caatacaggc aggttgcata ctggctacat aaatcagagc gtctcgtaaa
     6061 tcgctacact tgatttgctt gtactcagcc accctgaatt tgtttttggt tcttgcccag
     6121 atttcgccgt agctgagctt ggtgcgttcg tgcgtggctt ttaccgcatt ttgtagcata
     6181 ttttgctctt ctgctgtgag ggttggctcg ggaagtgcgg tctgattttc cgctgaattt
     6241 tgcaaacgct ccgctttgcc taagtgccag tagttgtaga gtgcctcaaa acactcttct
     6301 tggtagcaga tcactttttc ttttaaatct acccgcactt tttcaggatt aatgctaaac
     6361 aaccagccgt tgagtttttt gattggcata cagagcattt cataagtttt gccgtctgat
     6421 ccagttgttt tcatatgagt acaactgaat ttttctttgg aattctcaag ttttctttgt
     6481 tgagaacccc aagacaagcc tagtgcatca acaatcgggc gaactgccgt gtaaataaca
     6541 tcttcaactt ttaaagtaat aagatctgaa ccgtaaaaag aaagagtttg agtagtaatt
     6601 tgattagaca taagtctgct cctgtttgtt ttttcgaagt ttagttttcc ctattcaata
     6661 gggtgccgag aggttcgaaa accgcaaaca gacggtcggg attattcccc tttcgggtat
     6721 tgtattctcc gccctctcga cattgagcct gtacaatctt tttctaatgg ctggaaaaag
     6781 atcgcaaaat ttagacacaa aaaaaccgct tgtctgacgg gtgcggattg ccgctgtttg
     6841 tttaggtttc gacgcctatg gggaaatagt actactcaaa ttttggtttg taaagctatt
     6901 ttatcatctc aatcaacaca tttgctaacg gtgttacaca taaggcaaaa acgcccaacg
     6961 ctataagtac gattgaaata gctatcgcac cacgcataat aggacttgca tttgtttcca
     7021 tttctaaccc cttttttaag tggattttta actttgtttt gctataattg ctcataattt
     7081 atttaatcat tccgtattaa tggttaaatg aaaaacccca agagtttgcg gctcttgggg
     7141 tttgtttttg gtaataaaaa agcccattta acataatggg ctctactcta tagtagtttt
     7201 actcgttttt tatggttttc attttattat gaagcatatt tctgaactag ctttttaaaa
     7261 taaggtagtt cttttttaca aaactcaact ttgcttggtg aagattttag agcatttatg
     7321 aaatttaaag atgtttcctc ttgtaattta taagctacat tttcaggtaa ttgaggacag
     7381 ccattacgag ttgagtattc agcaaaacta tctccattta aattagaatt aaattctccc
     7441 agtttcgcct caacaagaca aatatcttga gcgtgtttgt gataaatcag atcttttatt
     7501 agctgattgc attgcttgtc agagattagt tgtttagctt ttacttctga cgctgtttta
     7561 ttccctgaag accaagctat aaaatctaat atcaagaaaa ttacaaatag agccaaaaga
     7621 gccttgatt
//
LOCUS       DANJWC010000120         7304 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.120, whole genome shotgun sequence.
ACCESSION   DANJWC010000120 DANJWC010000000
VERSION     DANJWC010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7304
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.120"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..452)
                     /locus_tag="PWX02_001824"
     CDS             complement(<1..452)
                     /locus_tag="PWX02_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005537801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL1729813.1"
                     /translation="MKNKCVKFTLATLATLVLSGTVMAKMTEGKLVIWINGDKGYNGL
                     AEVGKKFEKDTGVQVLVEHPDRLEEKFAQVASTGDGPDIMFWAHDRFGGYAQSGLLAE
                     VNVSKEFKDKFVDFAWDAETYNGKIIGYPVAIEAISLIYNKDLVKEAP"
     gene            810..1928
                     /gene="malK"
                     /locus_tag="PWX02_001825"
     CDS             810..1928
                     /gene="malK"
                     /locus_tag="PWX02_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005552148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HDL1729814.1"
                     /translation="MANVSLRNVGKSYGDVHISKDINLEINEGEFVVFVGPSGCGKST
                     LLRMIAGLEDITTGELYIGGKLMNEVEPSKRGIGMVFQSYALYPHLDVAENMSFGLKL
                     AGVSKAERDQRVNQVAEILQLAHLLDRKPKALSGGQRQRVAIGRTLVSQPEVFLLDEP
                     LSNLDAALRVQMRVEISKLHKKLNRTMIYVTHDQVEAMTLADKIVVLNAGGIAQVGKP
                     LELYHYPANRFVAGFIGSPKMNFLPVKVIAVEPERVKIELPDANHHNFWIPVSGEGVN
                     VGDNLSLGIRPEHLVPSDQTQVTLHGIVQVVELLGNETQIHLEIPEIKQPTLIYRQND
                     VVLVNEGDDLNIGILPERCHLFKEDGTACKRLFVEKGV"
     gene            2026..3315
                     /locus_tag="PWX02_001826"
     CDS             2026..3315
                     /locus_tag="PWX02_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin"
                     /protein_id="HDL1729815.1"
                     /translation="MKKTVLAVAISGAMFAASASAVDFHGYARSGIGWTSGGGEQSAF
                     TVNGGGSKYRLGNESDTYAELKLGQELFKSGEKSIYFDTNLAYGGTLHNNDWTPTSPA
                     LRELNVQFKNFADSLPGATLWAGKRFYQRHDVHMNDFYYWDISGPGAGVENIDLGFGK
                     LSLAVTRDTERGGANTFGYTTVYKLDPSTGKIKLDKQRNKADVYNDIFDVRLAGIELW
                     KDGSLELGFDYGNAHAKKGAALEKGATKNGYMVTAEYTQGNFFGGFNKFTAQYATDSM
                     TSWNNGHAQGSKADNKGNMLRLINQGVVQASDKVEVMYALIYEKTKLDNKQGKTWYSA
                     GIRPMYKWNDTMSTLLEVGYDRIKDQATGKKNDLVKYTVAQQWQAGNSIWARPAIRVF
                     GTYARWNDKFNTKARTDAGYKAKDGEFITGVQFEAWW"
     gene            3397..4293
                     /gene="malM"
                     /locus_tag="PWX02_001827"
     CDS             3397..4293
                     /gene="malM"
                     /locus_tag="PWX02_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HDL1729816.1"
                     /translation="MKKTLLSTAIFFAHLLVVFPSIANGSAGKSIHINSAELAQIQWQ
                     DVPFSQTVKTDLSEQHKQAFTTAFAGIASPVAAYRIPANQGTLEIEIESPVMDKNVFV
                     PTAVVLDSQFNVASTYPSSEFKLLEERGLKGSRLGAELNLTPAMNQDYIYLLIYTTQQ
                     DLAKTTMMPHPAKTYAKATGKQPPAINDIEVAHSLKGQIHINVTGSNGTKFIGLPTEI
                     FSSNKAATPAAKTAIPLPIKENPVAVKTAVDKDTEAYFNQAVMKAIKAKDINKAMNLV
                     NEAEKLGLTSPRQIFLKNVSSN"
     gene            complement(4453..6321)
                     /locus_tag="PWX02_001828"
     CDS             complement(4453..6321)
                     /locus_tag="PWX02_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL1729817.1"
                     /translation="MSGLVSPELIKTVVLLATSVTIVPLFKRLGLGSVLGYLVAGCLI
                     GPSGIGLFQDPAAVVHMAELGVVMFLFIIGLEMHPELLWAMRKAIFGRGFLQVAICGT
                     LLTLAGIYLLGFSKEVAFIAGMGFTLSSTAIVMQVLEDKGISSTPKGQRIVATLLFED
                     LAIVPLLASVAFLAPNQVDAESSTNWSAIGLSLVAVLILVASGKWLMNPIFRVISKAK
                     VREMMTAAALLVVLGSALLMEISGLSMAMGAFLAGVMLSESSFRHQLEADIEPFRGLL
                     LGLFFMGVGMSLDLSLVLNNWLWLLVIVAVYVIGKGLGIFIAASITRLTLNESIVRTT
                     IMAHGGEFAFVLFSAAATAGILTPDTHATLTAAVIVSMLLSPLLILIKQGVRKKKAKN
                     ESTNMTGVELPEDLEANVLVIGFGRFNQIVCQALLAKGLSVSVIDSNTDHIRAAARFG
                     FKLYYGDGARLDVLRASGIANANCVIVGVGNPERTEKIVELIKSEYPLVPVFARTYDR
                     QSAVDLVKKHHVDYHIRETFESALALSQAAIERLGSSEEEAKQIISYVRRLDAERFNE
                     ELLHGFSAEVIRKYWVPTPWVNPQHEGSALNEETADILEELGETVEVIKVDITDKN"
     gene            complement(6352..>7304)
                     /locus_tag="PWX02_001829"
     CDS             complement(6352..>7304)
                     /locus_tag="PWX02_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDL1729818.1"
                     /translation="EKLRQFITDLRLSFPIQAPEVTNDDMHHPNEIRNLVIALNLVQD
                     PTEHIDELPKKVYPSDLFNFGKAQKTLVGSVSIIYRNVWNEIRTQHFDGEDAILKALK
                     LVSNRIYQGNSSPQSVNVFCYSKYLRSELRDFVADLVHKCITIQTGCLLQENEFSTLK
                     VAGKTWQFLFHKKTFSIKQIDEQQAVENLNSFAKTKQNAKSENEKKKEFPQQISEFAS
                     EGFLQFFFEDNVDNSFNVYVVDERNNVESYYNCFGNKEEKIRKINKLQNEKQLKTETP
                     ASFGSFNHPQFYQLISPNGELCIVPFQSRQHLDYLAQKHH"
BASE COUNT         2315 a         1427 c         1431 g         2131 t
ORIGIN      
        1 tttggtgctt ctttcactaa atctttatta taaattaatg aaatcgcttc aatcgcaacc
       61 ggataaccga taattttgcc gttgtaagtt tcagcatccc aagcaaaatc gacaaattta
      121 tctttaaatt ctttgcttac attcacttca gccaataaac cggattgtgc ataaccacca
      181 aagcggtcgt gagcccagaa cataatatcc ggaccgtcac cagttgaggc aacctgtgca
      241 aatttttctt caagtctatc cgggtgttca actaaaactt gaacaccggt atctttttca
      301 aatttcttac cgacttcggc aagcccattg taccctttat caccgttaat ccagataacg
      361 agtttacctt ccgtcatttt tgccattact gttcctgaaa gaactaaggt agcaagagta
      421 gctaaggtga atttgacaca tttgtttttc ataaaaaact ccttgaagtt aaataaaaga
      481 aacggatatg aaacaaccca tttattaaat tatggcaaat tatgggctat acaatctatt
      541 tgagatcacc ctttgctcta cgccccccaa gctaaaagta tgatctaagt aacatttttt
      601 atcattttgt gatctgcttc acaaaaatag gcattaaaaa tgagaggttg atcacaaaat
      661 ttatttaatt tttctgaatt ggcgtttttt aaataattat ggctctaacc ctaaaaaaaa
      721 gtattttagg atgatttctt ctttagataa aaaggcgact attcagccta aataaaacca
      781 ataagcaaaa gcagaaaaga ggtttagtta tggcgaatgt atcattacgt aacgtgggaa
      841 aatcgtatgg tgatgttcat atctctaagg atattaattt agaaattaac gaaggagaat
      901 tcgttgtttt cgttggaccg tcaggttgtg gcaaatctac gctattacga atgatagcag
      961 gtttggaaga tattacgaca ggagaactct atatcggtgg aaaactgatg aatgaagtag
     1021 agccttcaaa acgtggtatc ggtatggtgt tccaatccta tgctctctat ccacatttag
     1081 acgttgccga aaatatgtct tttggcttaa agctggcagg tgtaagtaaa gcagagcggg
     1141 atcaacgagt taaccaagtt gccgaaatct tacagcttgc ccatttatta gatcgaaaac
     1201 ctaaagcact ttccggcgga caacgccaac gggtggctat tggtagaaca ctggtttccc
     1261 aaccggaagt atttttactc gatgaaccac tttccaacct tgatgccgca ttgcgtgtac
     1321 aaatgcgggt agaaatttcc aaattacata aaaaactaaa ccgcacaatg atttatgtta
     1381 cgcacgatca agttgaagca atgacattag cggacaaaat cgtggtatta aatgccggcg
     1441 gtattgctca agtaggtaaa ccgttagaac tttatcatta cccggcaaac cgctttgtag
     1501 cagggtttat cggctcgcct aaaatgaact ttttaccggt aaaagtgatt gcggtagagc
     1561 cggaacgggt aaaaattgag ttacctgatg cgaatcatca taatttttgg attccggttt
     1621 ccggcgaagg ggtgaatgtg ggagataact tatctttagg tatccgcccg gaacatttag
     1681 ttccttcgga tcaaacccaa gtgaccttac acggtattgt gcaagttgtg gaattgctcg
     1741 gaaatgaaac acaaatccac cttgaaatcc ctgaaatcaa acaaccaacc ttaatttatc
     1801 gtcaaaacga tgtggtatta gttaatgaag gcgacgatct gaatatcggt atattgccgg
     1861 aacgctgcca tttatttaag gaagacggta cagcttgcaa acgtttgttt gttgaaaaag
     1921 gggtataaca atttcagttt attactgatt ttagagggta ttttcaatct tctaatcaat
     1981 taataattaa cctaagtaaa atcaaatctt aagagaggtt ctattatgaa aaaaacagtc
     2041 ctagcagttg caatcagtgg tgcaatgttc gcagccagtg catcagcggt tgatttccac
     2101 ggatatgcac gttcaggtat cggttggaca tcgggggggg gggagcaatc cgcattcacg
     2161 gtaaatggtg gtggttcaaa ataccgttta ggtaacgaat ctgataccta tgcagaattg
     2221 aaattaggtc aggaattatt caaaagtggt gagaaatcta tctactttga tacaaacctt
     2281 gcttacggcg gtacgcttca caataatgat tggacaccaa caagtccagc actacgtgaa
     2341 ttaaatgtac agttcaaaaa ctttgcagat agtttaccag gtgccacctt atgggctggt
     2401 aaacgttttt accaacgcca tgatgtacat atgaatgact tctattactg ggatatttca
     2461 ggtccgggtg caggggttga aaatattgat ttaggcttcg gaaaactctc tttagcggtt
     2521 actcgtgata cagagcgtgg aggggcgaat acttttggat atactacagt ttataaatta
     2581 gatcctagca caggtaaaat taaattagat aagcagcgta ataaagccga tgtttacaac
     2641 gatatttttg atgttcggtt agccggtatt gagctttgga aagatggttc gttagagtta
     2701 ggctttgatt acggtaatgc acatgctaag aaaggtgcag cccttgaaaa aggagcaacc
     2761 aaaaacggtt atatggtaac agcggaatac actcaaggta acttctttgg tggtttcaat
     2821 aaatttaccg cacaatatgc aaccgattct atgacctctt ggaataacgg tcacgcacag
     2881 ggttcaaaag cagataataa aggcaatatg cttcgtttaa tcaaccaagg ggtagtgcaa
     2941 gcgagtgata aagttgaagt aatgtatgcc ttaatctacg aaaaaacaaa actagataat
     3001 aaacaaggta aaacttggta ttctgccggt atccgcccaa tgtacaaatg gaatgataca
     3061 atgagtacat tattagaagt aggttatgac cgtattaaag atcaagccac aggcaagaaa
     3121 aacgacttag tgaaatacac cgttgcacaa caatggcaag caggtaacag catttgggct
     3181 cgccctgcaa tccgtgtgtt tggtacttat gcacgttgga atgataaatt caacacgaaa
     3241 gctcgtaccg atgccggcta taaagccaaa gacggcgagt ttatcaccgg tgttcaattt
     3301 gaagcttggt ggtaatcatc aaacctttgt gtgggtagga ttacctaccc actatttcag
     3361 cactttatcc tagtttcacg atttttagag gttaatatga aaaaaacgct tctttcgaca
     3421 gcaatattct tcgcccatct tttggtggtg tttccgagta tagcaaatgg ctcggcaggt
     3481 aagtctattc atattaactc ggctgaatta gctcaaattc aatggcaaga tgtgccattt
     3541 tcacaaaccg taaaaacaga tctaagcgag caacacaaac aagcatttac cacagcgttt
     3601 gccggtattg ccagcccggt tgcagcttat cgtattccgg caaatcaagg cacattggaa
     3661 attgaaatcg aaagcccggt gatggacaaa aatgtttttg tacccacggc agtcgtgttg
     3721 gatagtcaat ttaatgtggc atcaacttac ccctcttctg agtttaaatt attggaagag
     3781 cgaggactca aaggtagccg tttaggggca gaacttaatt taacgccggc aatgaaccaa
     3841 gattatattt acttgctcat ttataccacg cagcaagatt tagccaaaac cacgatgatg
     3901 ccgcatccgg caaaaaccta tgcaaaagca accggtaaac aaccaccagc aattaacgat
     3961 attgaagtag cacacagttt aaaagggcaa attcacatca acgttaccgg ctcgaacggc
     4021 actaaattta ttggattacc gactgaaatt ttcagctcaa acaaagcagc aacgccggca
     4081 gctaaaacgg ctataccgtt gccgataaaa gaaaatccag ttgcagtaaa aacagcagta
     4141 gataaagata cggaagccta ttttaatcag gcggtaatga aagcgattaa agcaaaagat
     4201 attaataaag cgatgaattt ggttaacgag gcagaaaaat tagggctgac ttctccacgc
     4261 caaattttct taaaaaatgt aagttcaaat tgatttattg tttttttcat agatattctc
     4321 cttgtcatcg tgcgatggtt tattaccatt aacaccctat cagattatgc cgatagggtg
     4381 cttttttacg agtgattatg cgatatgtat aggaaggctt atacttgaat aaggattcaa
     4441 agtataaatt ctttaatttt tatcagtgat atccactttt ataacttcta ctgtctcacc
     4501 aagctcttct aaaatatcag ccgtttcttc attaagtgca ctaccttcgt gttgtggatt
     4561 tacccaaggt gttggtaccc aatatttgcg gataacttct gcggagaaac cgtgtaataa
     4621 ttcctcatta aagcgttctg catctaatcg gcgaacatag ctgataattt gttttgcctc
     4681 ttcctcgcta gaacctaagc gttcaatggc ggcttggctt aatgcgaggg ctgattcaaa
     4741 ggtttctcga atgtgataat caacgtggtg ctttttgact aaatcaaccg cactttgtct
     4801 atcataggtt ctcgcaaata caggtactaa ggggtattca gatttaatca gctctacgat
     4861 tttctccgtt ctttccggat tgcctacgcc aacaattacg cagtttgcat tggcgatgcc
     4921 agatgcccta agtacatcta gtctcgctcc atcaccatag taaagtttaa aaccaaaacg
     4981 agcagcagca cgaatatgat cagtatttga atcaataacc gatacactta gtcctttggc
     5041 taataaggct tggcagacta tttggttaaa gcgtccaaaa ccgatgacta atacattggc
     5101 ttctaaatct tccggtaatt caacccctgt catattagtg gattcatttt tcgctttttt
     5161 ctttctcact ccttgcttga ttaaaatgag aagtggggaa agcaacattg agacaattac
     5221 cgcagcggtt aaggtagcgt gagtatctgg ggttaaaatc ccagcggttg cggcggctga
     5281 gaatagcaca aaggcaaatt caccaccgtg agccataatg gtagttcgca caatagattc
     5341 attcagtgtt aatcgggtga tagaggcagc aatgaagatt ccaaggcctt taccgataac
     5401 ataaactgct acaatcacca ataaccatag ccaattattg aggacgaggc tgaggtctaa
     5461 tgacattcct acgcccataa agaataagcc taaaagtaaa ccacggaagg gttcaatatc
     5521 agcttctagt tgatggcgga aagaagactc cgaaagcatt actccggcaa ggaatgcccc
     5581 cattgccatt gaaagcccac tgatttccat taaaagagcg gagcctaata ccacaagtaa
     5641 ggcagcggca gtcatcattt ctcgtacttt ggctttagaa ataacacgga aaatagggtt
     5701 catcagccat tttccactcg caaccaaaat aagaacggca actaggctaa gaccaatagc
     5761 agaccaatta gtactggatt cagcatctac ttggttagga gcaagaaagg caaccgaggc
     5821 taataatggc acaatcgcta aatcttcaaa tagtaaagtt gcaacaatac gttgcccttt
     5881 tggcgtggaa cttatccctt tatcttccag cacttgcatt acaatggcag tagaagatag
     5941 cgtaaaaccc ataccggcaa taaatgccac ttctttggaa aaacctaata agtaaatgcc
     6001 ggctaaggtt aataaagtac cgcaaatcgc aacttgtaag aaacctcgcc cgaagatggc
     6061 tttacgcatc gcccaaagca gttccgggtg catttccaaa ccaataataa acaaaaacat
     6121 aaccacgcca agttctgcca tatgcacaac agcggcaggg tcttggaaca atccaatgcc
     6181 cgatggcccg attaaacatc ctgcgactaa ataacccaat acgcttccta aaccaaggcg
     6241 tttaaatagc ggtacaatag tgacgctggt tgcaagtaat acgactgttt taattaattc
     6301 cggactaact aatcctgaca tagaattccc acctgtataa tataaataag attaatgatg
     6361 tttttgtgct aaataatcta aatgctggcg gctttgaaac ggcacaatac ataactcacc
     6421 gtttggtgaa ataagttgat aaaattgtgg atgattaaag cttccaaatg atgctggtgt
     6481 ttctgttttt aattgttttt cattttgtaa tttattgatt ttcctgattt tctcttcttt
     6541 attgccgaaa cagttatagt aactttcgac attattacgt tcatctacaa catatacatt
     6601 aaaactatta tcgacattgt cttcaaagaa aaactgtaaa aagccttcac tggcaaattc
     6661 actaatttgc tgcggaaatt cttttttctt ttcattttcg cttttagcat tttgcttggt
     6721 ttttgcaaaa gaatttaaat tttcaaccgc ttgttgttca tcaatttgtt taatgctaaa
     6781 tgttttttta tgaaaaagaa actgccaggt ttttcctgct acttttaaag tgctaaattc
     6841 attctcttgc agtaaacaac cagtctgaat tgtaatacat ttatgaacta aatctgccac
     6901 aaaatcccgt aactcactac gtagatattt actatagcaa aaaacattaa ctgattgagg
     6961 tgaagaattt ccttgataaa ttcgattgga taccaatttt agtgctttta aaatagcatc
     7021 ttcaccgtca aaatgttgag ttcgaatttc attccataca tttcgataaa taatgctcac
     7081 actaccaact aacgtttttt gtgccttacc aaaattaaac agatcgcttg gataaacttt
     7141 ttttggtagt tcatcgatat gttcggttgg atcttgtact aaattaagcg ctataaccaa
     7201 attgcgaatt tcattagggt gatgcatgtc atcattggtc acttccggcg cttgaattgg
     7261 gaaagataag cgcaagtcgg tgataaattg tcgcaatttc tcga
//
LOCUS       DANJWC010000121         7185 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.121, whole genome shotgun sequence.
ACCESSION   DANJWC010000121 DANJWC010000000
VERSION     DANJWC010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7185
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.121"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..2512
                     /gene="topA"
                     /locus_tag="PWX02_001830"
     CDS             <1..2512
                     /gene="topA"
                     /locus_tag="PWX02_001830"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDL1729819.1"
                     /translation="HIRDLPKSGSDEKTEKAKPISTKGLTAAEKAKIKAEKERVALVK
                     RMGIDPYNGWKANYQIMPDKEKVVSELKSLAKKADHIYLATDLDREGEAIAWHLREII
                     GGDDSRFSRVVFNEITKDAIKKAFDDPQPLNIDRVNSQQTRRFLDRVVGFMVSPLLWK
                     KVARGLSAGRVQSVAVKLLVEREREIKAFQSEEFWTILANTQINRKKLPLELTTYKGK
                     KFSASNEAEANVAVRALEQSDFIVSNIEAKETSSRAAAPFITSSLQRAASTRLGFGVK
                     KTMMLAQRLYEAGYITYMRTDSTNLSREALAMARGYIQDNFGANYLPEKANVYRSKDN
                     AQEAHEAIRPSDVNVKMADLKGMDKDAERLYDLIWRRFLACQMLPARYDSTAVTVSAG
                     DYELKANGRVLRFDGWTKVLPVQGKNPEDQELPAVELHQKLTLDEIVPSQHFTKPPAR
                     FNEASLVAELEERKIGRPSTYASIISTIQERGYVRTENKRFYVEKIGEVVTDRLNESF
                     KDLMNYDFTANMEEVLDQIAEGSKNWKEELNTFFKHFSEELSQAELDELEGGMKPNSL
                     VPTDIDCPTCGRKMAIRTASTGVFLGCTGYALPPKERCKTTMNLIPEAEFFNVLDSDS
                     ETKALMARKRCPKCDSAMDSYVIDAERKLHVCGNNPNCDGHIIEQGSFKIKGYDGPVV
                     ECDKCGSDMHLKLGRFGKYMGCTSCDNTRKILKNGEVAPPKEDPIAFPELKCEKADAY
                     FVLRDGASGVFMSAHNFPKVRESRAPKVVELAQYRERLPEKLQYLADAPQQDPEGNEA
                     IIRFSRKEKRQYVTSEKNGKATKWIVDYLDGKWVERKK"
     gene            complement(2562..3500)
                     /gene="yvcK"
                     /locus_tag="PWX02_001831"
     CDS             complement(2562..3500)
                     /gene="yvcK"
                     /locus_tag="PWX02_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HDL1729820.1"
                     /translation="MENTDIIPFKPTRHHYLNQLNAVVALGGGHGLGRLLSALSFLKE
                     RLTGIVATTDNGGSTGRIRSQQGVIAWGDLRNCLNQIIVKPTIASRLFEYRFAGTGEL
                     AGHNLGNLILTALENMQIRPLEGINLVRELLHVRSQLIPMSEIPVHLIAQLHSGESVF
                     GEVQIDSLAETPQHLALDTMVVATPEAISAIQSAELILLGPGSFFTSIMPNLLVAGIA
                     NSIAQSKARVVFIDNIGQEHGPAGQLSLNQRIEWMENCIGQARINAVITTDDAGSELP
                     EHIQILRKNLSANDVYYRHDRNKLSKAIDELLAQNI"
     gene            complement(3514..4590)
                     /locus_tag="PWX02_001832"
     CDS             complement(3514..4590)
                     /locus_tag="PWX02_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL1729821.1"
                     /translation="MFQMLQDWYRKKFTDPQIVVLFSILLIGFGIIYFFSDLLMPLLV
                     ALVFAYLLEWPIRFLSSKLKLPRTLSVILVLGGFIALLSFLGVVLLPSLWNQAVTFIQ
                     DLPSMFNLLNAWLQALPEHYPELVDYATLDSIVNTAKSNILSMGESLLTLSINSIISL
                     VGLGIYTFLVPLMVFFLLKDKIVLMRSFSKVLPQNRRLATRVWFEMQQQIANYIRGKF
                     LEILIVGVVTYIIFLFFDLRYPLLLSVAVGISVLVPYIGAVLVSIPVMLIALFQFGLS
                     PDFYYLMLAFIISQLLDGNLLVPFLFSEAVNLHPLTIIIAVLIFGGLWGFWGVFFAIP
                     LATLVKAVLQALPSAQSDAIILEK"
     gene            complement(4621..5376)
                     /locus_tag="PWX02_001833"
     CDS             complement(4621..5376)
                     /locus_tag="PWX02_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL1729822.1"
                     /translation="MKKEEVGHHFLARLGKTRLRPGGKLATDWLIANGDFDKNKKVLE
                     VACNMGTTAIQLAQQFECQIVGIDLDEDALEKARKNIKENHVEHLIQVQRANATKLPF
                     DDESFDIVINEAMLTMLPLEAKEKAIREYFRVLKPNGFLLTHDIVIQDKDSDTLLAEL
                     RDAINITVSPLAKADWKSLFQRCGFRNVDTFSGEMSLLSPKGLIHDEGVLGAMKIIGN
                     ALKPENRETFNKMYKLFNDPDKKLGFIAVCSQK"
     gene            5528..5737
                     /locus_tag="PWX02_001834"
     CDS             5528..5737
                     /locus_tag="PWX02_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome alternative rescue factor ArfA"
                     /protein_id="HDL1729823.1"
                     /translation="MSKQKNDYLHQRGQIKESAVKALVTDPLFRQRVERNRKGKGSYR
                     RQEKHKKTIYGQKETPFKNIRLESF"
     gene            complement(5786..6508)
                     /gene="bioD"
                     /locus_tag="PWX02_001835"
     CDS             complement(5786..6508)
                     /gene="bioD"
                     /locus_tag="PWX02_001835"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HDL1729824.1"
                     /translation="MPSLFITGTDTNVGKTVVTRAIIQTLTKHSFPVIGYKPIACGGD
                     DSLPTEPTQYDYATEDNSDVLTILDSCPTNVSYRDINSYTFIHSSTPVFAALDAVHHI
                     QLEKLNTDLKRLENAYPNVVVEGTYGWLTPINKDYYFADWVMQNNMPVVLVVGIKEGC
                     VNHALLTANEILQRGVKLVGWVANRVNPGLRHYHELIELLTQKINAPLLGQISYMGHP
                     EHKELSSYIQNPEPLLAYFSKP"
     gene            complement(6556..>7185)
                     /locus_tag="PWX02_001836"
     CDS             complement(6556..>7185)
                     /locus_tag="PWX02_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL1729825.1"
                     /translation="QLWLLAESTVGSLINQDNAIFLQLDDTVNLSVLLSGSLLSKHSK
                     MNVDNMLMPRFSHLSDEIFPELDEIHRYKLVNQVTFPAIVRLIDRYLPNNLKSQEQKI
                     SWLCQKIDENQPLALAILSHITDNLAYALMNLVNIFSSEKIMLNSPLIKIKEPLFTQI
                     SEKLHKNLLQTNLKIDLVTSQYDWNSPLIACSAIKQGIYDGNLIKDRIN"
BASE COUNT         2281 a         1329 c         1491 g         2084 t
ORIGIN      
        1 gcatattcgt gatttaccga aaagtggtag tgatgaaaaa acggaaaaag cgaaaccgat
       61 ttcaaccaaa gggctgaccg ctgccgaaaa agctaaaatc aaagcagaaa aagagcgtgt
      121 tgcattggtt aaacgtatgg gtatcgaccc ctataatggc tggaaagcta actatcagat
      181 tatgccggat aaagagaaag tggtttccga gctaaaatcg ttggcaaaga aagcagatca
      241 tatctatctg gcaaccgact tggatagaga aggggaggcg attgcgtggc atttgcgtga
      301 aatcattggt ggtgatgaca gccgttttag ccgagtggta tttaatgaaa tcaccaaaga
      361 tgcgatcaaa aaggcttttg acgatccgca accgctcaat attgaccgtg taaattccca
      421 gcaaacccgc cgctttttag accgtgtagt gggctttatg gtgtcgccat tattatggaa
      481 aaaagtagct cgtggtttat cggcagggcg tgtgcaatcg gttgccgtaa aattactggt
      541 tgagcgtgag cgtgaaatca aggcgttcca aagcgaggag ttttggacta ttcttgccaa
      601 tacgcaaatt aatcgtaaaa aattaccgct tgaattaact acttataaag gtaaaaaatt
      661 ctcggcaagc aatgaggctg aagcaaatgt tgcagtgcgt gctttggagc agagcgattt
      721 tattgtttct aatattgaag ccaaagaaac cagctctcga gctgctgcac catttattac
      781 ctcatcgtta caacgtgccg ctagcactcg tttaggtttt ggggttaaaa agacgatgat
      841 gttagcccaa cggttatatg aagcgggtta catcacctat atgcgtactg attcaaccaa
      901 ccttagccgt gaagcattag caatggctcg aggttatatt caggataatt tcggggcgaa
      961 ttatttgccg gaaaaagcaa atgtgtatcg cagtaaagat aatgcccaag aagctcacga
     1021 agccattcgc ccgtcggatg tgaatgtcaa aatggcggat ttaaaaggaa tggataaaga
     1081 tgccgagcgt ttatacgatt taatttggcg tcgtttcttg gcgtgtcaaa tgttgcctgc
     1141 tcgttatgat tctaccgcag tcacagtgtc tgccggagac tatgaactca aagcaaacgg
     1201 gcgtgtactg cgttttgacg gctggacgaa agtactgccg gtgcaaggca aaaatccgga
     1261 agatcaggaa ttaccggcgg ttgaactgca tcaaaaatta acgcttgatg aaattgtgcc
     1321 aagtcagcat tttactaaac cgcctgcacg ttttaatgag gcgtctttgg ttgctgagtt
     1381 agaagagcga aaaattggcc gcccttcaac ctatgcttca attatttcga cgattcaaga
     1441 acgtggctat gttcgcaccg aaaacaagcg tttttatgtg gaaaaaatcg gtgaagtagt
     1501 gacggatcgc ctcaacgaat cattcaaaga tttaatgaat tatgatttca ctgccaatat
     1561 ggaagaggtg ctcgaccaaa ttgcagaggg gtctaaaaat tggaaagaag agttgaatac
     1621 tttctttaaa catttctctg aagagttgag ccaagcagaa ttagatgaac ttgaaggcgg
     1681 aatgaaacca aacagccttg tgccaaccga tattgattgc ccgacttgtg gacgaaaaat
     1741 ggcaattcgc accgcttcca caggggtatt tttaggttgc accggctatg ctttaccgcc
     1801 aaaagagcgt tgcaaaacga caatgaattt aattccagag gcggaatttt tcaatgtgtt
     1861 agattcggat tctgaaacca aagccctaat ggctcgcaag cgttgcccga aatgcgattc
     1921 ggcaatggat agttatgtga ttgatgctga acgtaaatta cacgtttgtg gtaataatcc
     1981 aaattgtgat ggtcatatta ttgagcaagg ttccttcaaa attaaaggct atgacggacc
     2041 tgtagtggaa tgcgataaat gcggttcaga tatgcactta aaactgggtc gtttcggcaa
     2101 atatatgggc tgcacaagct gcgataacac ccgtaaaatt ctgaaaaacg gggaagttgc
     2161 tccgccaaaa gaagatccta ttgcttttcc tgaattaaaa tgtgaaaaag ccgatgccta
     2221 ttttgtatta agagacggag caagtggggt gtttatgtca gctcataact tcccgaaagt
     2281 gcgtgaaagc cgtgcaccaa aagtggtaga gttagctcaa tatcgtgaac gtttgccgga
     2341 gaaattgcag tatcttgccg atgctccgca gcaagatccg gaaggaaacg aggcaattat
     2401 ccgttttagt cgtaaagaga aacgccaata tgtgacttct gagaaaaacg gcaaagccac
     2461 caaatggatt gttgattatc tagacggtaa atgggttgag cgtaagaaat aaagcttaaa
     2521 agacaagcgg taaaattttc taaaaatttt accgcttgct tttagatgtt ctgagcgagt
     2581 agttcatcaa ttgctttact taatttattg cgatcgtgtc ggtaataaac gtcattcgca
     2641 gataagtttt ttcgcaagat ttgaatatgt tcgggaagtt cagaaccggc atcatcagtc
     2701 gtaattaccg cattaattct agcctgtccg atacagttct ccatccattc aattcgctga
     2761 ttaagtgata gttgcccagc tgggccatgt tcttgtccga tattgtcaat aaatactact
     2821 cgggctttgc tttgagcaat agagtttgct atgcctgcca ccagcaaatt cggcataata
     2881 ctggtaaaaa agctacctgg gcctagcagt attaactcag cactttgaat agctgaaatg
     2941 gcttctggtg tagcaacaac catagtatct aacgctaaat gttgtggtgt ttctgctaaa
     3001 gaatcaattt gcacttcacc aaatactgat tcgcctgaat gcaactgtgc aattaaatgc
     3061 accggtattt cagacattgg gataagttgc gatcttacgt gcaataattc tcgcaccaaa
     3121 ttaatccctt ctagcggtcg gatttgcata ttttcaaggg cggtgagaat taaattgcct
     3181 agattatgtc cagcgagttc gcctgtgcca gcaaaacgat actcaaacaa ccgagaagct
     3241 atcgttggtt taacgataat ttgatttaaa cagttgcgta aatctcccca agcaatcacc
     3301 ccttgttgtg aacgtattct accggttgag cctccattat cggtagtggc aacaatgccg
     3361 gtaagccgtt cctttaaaaa agagagcgcg gagagcaatc gacctaagcc gtggccccca
     3421 cctagtgcaa caactgcatt gagttggttt aaatagtgat gtcgggtagg tttaaaagga
     3481 ataatatctg tattttccat cttgggattt cgtttatttt tctaaaataa tggcatctga
     3541 ctgggctgaa ggcaatgctt gtaatacagc tttaaccaaa gtcgctaaag gaatcgcaaa
     3601 aaacactccc caaaagcccc ataaaccacc aaagatgagt acggcaataa taattgtcag
     3661 agggtgtaga tttactgctt cagaaaataa aaagggtacg agtaaatttc cgtctagcaa
     3721 ctgactaata ataaatgcga gcattaaata gtagaaatcc ggcgataacc cgaactggaa
     3781 tagagcaatt aacattaccg gaatgctgac taaaaccgca ccgatgtagg gaactaacac
     3841 tgaaatccct accgctacag aaagtagtaa cggatagcgt aaatcaaaaa agaggaaaat
     3901 aatataagtg acaaccccaa caattagaat ttctaaaaat ttacctcgaa tatagttggc
     3961 aatttgttgt tgcatttcaa accatactcg tgttgcaagt cgacggtttt gcggtaatac
     4021 tttactaaaa gaacgcatta aaacgatttt atctttcagt aagaaaaaga ccatgagtgg
     4081 gactaagaag gtgtaaatgc ctaagccaac taagctgatg atagagttaa tcgagagtgt
     4141 tagaagtgat tctcccatac tcaaaatatt acttttagcg gtattcacaa tggaatctag
     4201 ggtcgcataa tctactaatt cagggtaatg ctccggtagg gcttgtaacc aagcattgag
     4261 cagattaaac atcgaaggta aatcttgaat aaaggtaact gcttgattcc aaaggctggg
     4321 tagtagtact acgcctaaaa aagataataa agcgatgaaa cctcctagta ctagaatgac
     4381 actcaaagtt cgaggtaatt tgagtttgct cgacaaaaaa cggattggcc attccaataa
     4441 ataggcaaac acaagtgcaa ccagaagggg cataagcaga tcgctaaaaa aatagataat
     4501 accgaaaccg atcaatagaa tactaaacag cacaactatt tgcgggtcgg taaatttttt
     4561 tcgataccaa tcttgcaaca tttgaaacat atggattgcc tttaaattat gcgatgaaaa
     4621 ttatttctgg ctacagactg caatgaaccc tagctttttg tccgggtcat taaatagttt
     4681 gtacatttta ttaaaggttt cgcgattttc tggttttaat gcattgccga taattttcat
     4741 ggcgcctaaa acaccttcat catgaatcag acctttaggg gaaagtagag acatttctcc
     4801 ggaaaaggta tcaacattac ggaaaccaca acgttggaat aatgatttcc agtctgcttt
     4861 agcaagtgga gagacagtga tattgattgc atcacgcaac tcggctaata aagtatcgga
     4921 gtctttatct tggataacaa tatcgtgcgt taataaaaag ccatttggtt ttaatacacg
     4981 aaaatattca cgtattgcct tttctttcgc ttctagagga agcattgtca gcatggcttc
     5041 attaattaca atatcaaaac tttcgtcgtc aaatggcagt tttgttgcgt tggcgcgctg
     5101 tacttgaatt aaatgctcaa catggttttc tttgatattt ttacgtgctt tttcaagcgc
     5161 atcttcgtct aaatcaatac caacaatttg gcattcaaat tgttgagcta attggattgc
     5221 agtagtcccc atgttacagg caacttcgag cactttttta tttttatcaa aatccccgtt
     5281 tgcaattaac caatcagttg ctaatttccc cccggggcgt aaacgagttt tgcctaaacg
     5341 agctaaaaaa tgatgaccaa cttcttcttt tttcataaaa acctctggat ttgcaaaata
     5401 cgttaaatat atgaccgctt gtattgtgat ctatgttcgt tattatactc aattttttca
     5461 ttttcttata tacaacatca acagattttg ccagaataca acctcatttt gatatgagga
     5521 aaatagaatg agtaagcaaa agaatgatta cctccaccag cgaggtcaaa ttaaagaaag
     5581 tgcagtaaaa gcattagtga ctgatccact ttttagacaa cgtgtagaaa gaaatcgtaa
     5641 agggaaaggg agctatcgca gacaagaaaa acacaaaaaa actatctatg ggcaaaaaga
     5701 aaccccattt aaaaatattc ggttagagtc tttttaaatg gggttttatt tgtagcttat
     5761 tgtagtttta taagcctgat aaacactatg gtttactgaa ataggccaac aacggttctg
     5821 gattttgaat ataagaagaa agttctttat gctctgggtg acccatatag gaaatttgcc
     5881 caagtaaagg cgcattaatt ttttgtgtca gtagttcaat taattcgtga taatgccgta
     5941 gccctggatt tactctgttt gcgacccaac ctactaattt tacaccacgc tgcaaaattt
     6001 cattcgcagt aagtagtgca tggttaacac aaccttcctt aattcccacc actaaaacaa
     6061 ccggcatatt gttttgcatt acccaatcag caaaatagta atccttgtta ataggagtta
     6121 gccagccata ggttccttct acgacaacat ttggataggc attttctagt cgttttaaat
     6181 ctgtgtttaa tttttccagt tgaatatgat gaactgcatc taaagctgca aatacagggg
     6241 tactggaatg aataaaagta tagctattaa tatcacgata gctaacatta gttggacagc
     6301 tatctagtat ggttaatacg tctgaattat cttcagtagc ataatcatat tgagtaggtt
     6361 ctgtgggtaa tgaatcatca ccgccacaag caatgggttt atagccaatt acaggaaaac
     6421 tatgttttgt aagtgtctgt atgatggctc gggtaaccac agttttccct acgttggtgt
     6481 ctgttccggt aataaataat gagggcataa atggctcctt tcgaaaatat ggctggtagt
     6541 ttaaccaaaa atagatcaat ttattctgtc tttgatcaaa tttccgtcat aaattccttg
     6601 ttttattgct gaacaggcaa ttaatggact attccaatcg tattggctgg taactaaatc
     6661 gatttttagg tttgtttgta acaaattttt atgcaatttt tctgaaattt gcgtaaataa
     6721 cggctctttt attttgatca atggcgagtt tagcataatt ttttcactag agaatatatt
     6781 aactaaattc attaatgcat aagctaaatt atcggtaata tgcgataaaa tagctaatgc
     6841 aagcggttga ttttcatcaa ttttttgaca aagccagctg attttctgtt cctgactttt
     6901 taaattatta ggcaaataac gatcaatgag tctcacaata gctgggaacg tcacctgatt
     6961 gactaattta tagcgatgaa tttcatctag ttcagggaat atttcatcac tgagatggct
     7021 aaatctaggc ataagcatat tatcaacatt catctttgag tgtttagaca gtaaactacc
     7081 actaagcaat acacttagat tcacagtatc atctaattgt agaaaaatag cattatcttg
     7141 gttaattaat gagccaaccg tagattctgc aagcaaccac agctg
//
LOCUS       DANJWC010000122         7036 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.122, whole genome shotgun sequence.
ACCESSION   DANJWC010000122 DANJWC010000000
VERSION     DANJWC010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.122"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(345..938)
                     /locus_tag="PWX02_001837"
     CDS             complement(345..938)
                     /locus_tag="PWX02_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729826.1"
                     /translation="MHNNLAIFYCGTNGAGKSTLRSFNQDKVEIVIDSDNIAMQINPL
                     NPRLADIEAGRKAIELFQFAVKNQVAFSMESTLSGKSILKRMENAKAGYFKTRLNYIG
                     VDSYKINIARVKARVKAGGHFIDEETIRKRYHISRENLIEAIFINDETLIYDNSETKP
                     NLILQIADKKVIKISDKIPNWCNQLCDELVELGFKTT"
     gene            complement(928..1098)
                     /locus_tag="PWX02_001838"
     CDS             complement(928..1098)
                     /locus_tag="PWX02_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729827.1"
                     /translation="MTSREELLKKQRELDILFTAWFEEKKKHEVLTYRRENGDLIQHY
                     PDGTEKVIEYAQ"
     gene            complement(1100..1846)
                     /locus_tag="PWX02_001839"
     CDS             complement(1100..1846)
                     /locus_tag="PWX02_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDL1729828.1"
                     /translation="MNKLAKIEQLKTALSQRRPLSESEVQRLREEFIIESSYNSNAIE
                     GNTITLRETVLILKEGMTIAEKPIWEHLDIIGFKDAFNYIYELVANKEPLSERAIKDI
                     HSLVLMGNAESRGVYRKIPVRILGAENEPPPPYLIAEEMQLLIEKYQQKLAALHPIEA
                     ISWLHLAFESIHPFIDGNGRTGRLLINFELLKQGYLPIDIKFTNRAQYYQCFDDFHKN
                     QQDISSLVELISQYELAELERYLAILKQRT"
     gene            complement(1843..2286)
                     /locus_tag="PWX02_001840"
     CDS             complement(1843..2286)
                     /locus_tag="PWX02_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1729829.1"
                     /translation="MLKNLSWHAKRFDELSTQELFEIYHARTAVFVVEQNCPYQEVDN
                     KDLQAVHFFAKNAKNLTAYCRLIPANDGVHIGRVLVVKENRGSGLARELVQKAMDYCG
                     EHFPAQPIHAQAQSYLQGFYESFGFKAVSEVYLEDGIAHLDMVLE"
     gene            complement(2280..2717)
                     /locus_tag="PWX02_001841"
     CDS             complement(2280..2717)
                     /locus_tag="PWX02_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HDL1729830.1"
                     /translation="MKQVQFYLLSQADAAELSAAEMQACELAAQAWRLSKRVLIACET
                     EQQALNIDEALWARDPDSFVPHNLSGEITTYPTPIEISWKGKRNAQRRDLLISLQTQL
                     PEFIGSFTQIIDFVPVEETAKAQARDRYKQYRQLGWQLETIQC"
     gene            2956..3399
                     /gene="lysM"
                     /locus_tag="PWX02_001842"
     CDS             2956..3399
                     /gene="lysM"
                     /locus_tag="PWX02_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HDL1729831.1"
                     /translation="MGLFDFVGDIGRKLFNKEEDASKAVTEHLAEDNPGVENVNVTVE
                     NGVAKISGIASTAAAVEKAVLMAGNVAGITEVNIDEVTLPDDVQLASDDELYVIQKGD
                     TLWEIATKAYGNGAKYKAIVEANKEVIKDENKIFPGQKIRIPKGL"
     gene            complement(3453..4304)
                     /locus_tag="PWX02_001843"
     CDS             complement(3453..4304)
                     /locus_tag="PWX02_001843"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005701564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDL1729832.1"
                     /translation="MKQYLDLCNRIVSEGKWVANERTGKHCLTVINADLTYDVEKGEF
                     PLVTTRRSYWKAAIAELLGYIRGYSNAADFRQLGTKSWDANANENAQWLANPYRKGED
                     DMGLVYGAVGRNFPKPDGGSVDLLRQIVDDLKKGIDNRGEIYTFYHPGAFHMGCLRPC
                     LHSHHFSLLDGTLYLNSTQRSADVPLGLNWNMIQCYTFLALMAQITGHKAGQAFHKIV
                     NAHIYEDQLELMRDVQLKREPLNAPKLIINPDIKSLEDLETWVSLDDFKVEGYEYHPA
                     IQYPFSV"
     gene            4391..4948
                     /locus_tag="PWX02_001844"
     CDS             4391..4948
                     /locus_tag="PWX02_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729833.1"
                     /translation="MDMKGIMKSILGFSTAVFLAACSSNKSGWTRLNDNDVDQKSYAI
                     AYGATAQTYADRVNESYDINSFMNGVNDWYTKKVTLPIEQIRAITINRMLDHNVYAYY
                     SGVIYAADLQANFNHLDPNCWGLVQPASMTQGIHDAMLDLQKNKVRGDEYIKNGVEQI
                     LHLCVKTIAEDENKSQSKGKTKKHK"
     gene            5157..5251
                     /locus_tag="PWX02_001845"
     tRNA            5157..5251
                     /locus_tag="PWX02_001845"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5192..5194,aa:Ser,seq:gct)
     gene            5265..5341
                     /locus_tag="PWX02_001846"
     tRNA            5265..5341
                     /locus_tag="PWX02_001846"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5299..5301,aa:Arg,seq:acg)
     gene            5471..6964
                     /gene="mltF"
                     /locus_tag="PWX02_001847"
     CDS             5471..6964
                     /gene="mltF"
                     /locus_tag="PWX02_001847"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HDL1729834.1"
                     /translation="MKGLIARLFIAVALLLWAWDMIFPWQKIIHSEANHYTQIQQSKT
                     LKVGMINHPLSYFVGPEGKSGIEYDLATAFAQYLKVNLDIKTYDSSEKLFEALRNNDV
                     DIAAAGLLYHSELNDEFQIGPSYYSASWQLVYKKGSARPYRLSDLRENVIIPAGSAVI
                     PILNQLKVDNPELKWQISDKFTQEELLLKVAQGEISHTIAMSVDISAAQHINPNLAVG
                     FDLTDENPVLWYLANSSYSELQSVLLDFMSYSMETGLISRIEEKYFNHLAKFDYVDIQ
                     SYLNAIKTILPKYEPLFRAHKGELEWQMLAAIAYQESHWDPNATSPTGVRGIMMLTKD
                     TADRMKISDRTNPAQSIKAGSEYLHMLMRQMPETIPQADRIWYSLAAYNMGLGHLLDL
                     RRLTKQLGGDPDNWLDVKKNLPLLAEKRYYANLKYGYARGYEALQYVENIRRYYGSII
                     NYQRVEEQKMQEQENLPDGQIKQENSSYEKIEEKEIPTDKEITPASN"
BASE COUNT         2123 a         1284 c         1392 g         2237 t
ORIGIN      
        1 gaataatagg aattttgtta ttttttatga actgttttaa gccattctct tttttatgag
       61 taccaataac ttttttagac tttttcctat tctgtttcat ctttgtgttt ttgttccaat
      121 atccccgatg gtgatcgtct tcgacgagag cttatttatt taagaaccta tcagagatag
      181 gcataagcgg tctgtttcga gaagtttttt gcaaagtttt tctttaatcc gacagcttgt
      241 cacccacaca caagccgtgt ggggttacgc atagctgagc cactttgtgg ctctaagtta
      301 cagctccaaa ggagctggga ttgtcgagcc tagcacggct cgtgctaggt ggttttaaac
      361 cctaattcca ctaattcatc acaaagctga ttacaccaat tcggtatttt gtctgaaatt
      421 tttatcactt tcttatcagc aatctgcaaa atcaaattcg gcttggtttc tgaattatca
      481 taaatcagtg tttcatcatt gataaaaatc gcttcaatta aattttcacg gctaatgtga
      541 tagcgttttc taatggtttc ttcatcaata aaatgtccgc cggctttcac tcttgctttt
      601 acccgtgcga tgttaatttt atagctatcg acaccaatat aatttaatcg agttttgaaa
      661 tatcctgctt tggcattttc cattcgtttt agaatagatt tacccgagag ggttgattcc
      721 attgaaaatg caacctgatt ttttactgca aactgaaata gttcgattgc ttttctgccc
      781 gcctcaatat cggccaatct tggattgagt gggttaatct gcatcgcaat attatcggaa
      841 tcaatcacaa tctcgacttt atcttgattg aaagaacgta aagtagattt gccggctccg
      901 tttgtgccac agtaaaaaat tgctaggtta ttgtgcatat tcaatcactt tttccgtacc
      961 gtccggataa tgctgaatta aatccccatt ttctcggcga tacgtcagca cttcgtgctt
     1021 tttcttttct tcaaaccatg ctgtaaagag aatatccagt tcacgttgtt ttttcagtaa
     1081 ttcttcacga cttgtcattt tatgtcctct gtttcagaat cgccaaatac ctttccaact
     1141 cagctaattc atactgacta atcaattcaa ccaaactact aatatcttgt tgatttttat
     1201 ggaaatcatc aaaacattgg taatattggg ctcgattggt aaatttaata tcaatcggta
     1261 aatagccctg ttttaataat tcaaaattaa tcagtaatcg ccctgtgcgc ccgtttccat
     1321 caataaaagg gtgaatgctt tcaaaggcta aatgcagcca actgattgcc tctatgggat
     1381 gaagtgcggc cagtttttgt tgatattttt caatcaataa ttgcatttct tcggcaatta
     1441 aataaggggg ggggggttca ttttctgccc ctaaaattcg tactggaatt ttgcgataaa
     1501 cccctcggct ttccgcattg cccattaata ccagagaatg aatatcttta atcgctcgtt
     1561 cgcttaatgg ttctttattg gcgactaatt cataaatata gttgaatgca tccttaaaac
     1621 cgataatatc taaatgctcc caaatcggtt tttcggcaat ggtcattccc tcttttaaaa
     1681 tgagcaccgt ttcacgcaaa gtaatggtat tgccttcaat tgcattggaa ttatagctgc
     1741 tttcaataat aaactcttcc cgcagtcgtt gaacttcgct ttcagataat ggacggcgtt
     1801 gtgaaagtgc tgtttttaat tgttcgattt tagctaattt attcattcta acaccatatc
     1861 caaatgcgca atcccatctt ccaaatacac ttctgaaacc gctttaaacc caaaactttc
     1921 ataaaaccct tgcaggtaac tttgggcttg ggcgtgaatg ggttgagcag gaaaatgttc
     1981 gccacaataa tccatcgctt tttgtaccaa ttcccttgct aagcctgagc cacgattttc
     2041 tttcaccacc aacactctac ctatatgcac accatcattg gctggaatta aacggcagta
     2101 agcggtcaga ttttttgcat tttttgcaaa gaaatgcacc gcttgcaggt ctttattatc
     2161 gacttcttgg taagggcagt tttgctcaac cacaaacacc gctgtacgtg cgtggtaaat
     2221 ctcaaacaac tcttgggtgg atagttcatc aaagcgtttt gcgtgccaag agaggttttt
     2281 tagcattgta tcgtttccag ttgccaacca agttggcggt attgtttata gcggtcacga
     2341 gcctgtgctt tggcggtttc ttccaccggc acgaaatcaa taatctgggt aaaactgccg
     2401 ataaattcgg ggagttgggt ttggagtgaa atcaacaaat cccgacgttg agcattgcgt
     2461 ttacctttcc acgaaatttc gataggcgtt gggtaagtgg tgatttctcc cgataaattg
     2521 tgcggcacga acgaatccgg atctcttgcc caaagtgctt cgtcaatatt taacgcttgt
     2581 tgctcggttt cgcatgcaat cagtacccgt ttacttaatc gccacgcttg agccgcaagt
     2641 tcgcaggctt gcatttcagc agcggaaagc tcggctgcat cggcttggct taatagataa
     2701 aattgtactt gtttcacaga atgttcccag aaattcaatc agatatttaa acttaatatt
     2761 ctagctgaaa atgagtaaaa tataaaatct aagcgtttgt ttttgcaaaa aattgagaaa
     2821 aaatgaccac ttgtgttgcg ttgccttgag gaactaatta gcttttagac gttctaagag
     2881 aacaatgcac tatttcaata gttggaaatt gtgtagaata cccacttcat ttagctattt
     2941 tataaggaac ttactatggg cttatttgat tttgttggcg acattggtcg taaattattt
     3001 aataaagaag aagatgcttc aaaagcggtt acagagcatt tggcggaaga caatccgggc
     3061 gttgaaaatg taaacgtaac ggttgaaaac ggggtagcga aaatttcagg cattgcttca
     3121 accgcagcag ctgttgagaa agcggtattg atggcgggta atgtggctgg tatcactgag
     3181 gtaaatattg atgaagtgac cttgcctgat gacgtgcaac ttgcaagtga tgatgagctt
     3241 tatgttatcc aaaaagggga tacgttgtgg gaaatcgcca caaaagctta tggtaacggt
     3301 gcaaaatata aagcgattgt agaagcaaat aaagaagtta ttaaagacga aaataaaatc
     3361 ttcccagggc aaaaaattcg tatcccgaaa ggtttataat caaattgaaa gagctaagct
     3421 ggtcgaaagt ttagctcttt ttgtaggtgg tgttagactg aaaatggata ttgaatggca
     3481 gggtggtatt cataaccttc aactttgaaa tcatctaagc tcacccaagt ttctaaatct
     3541 tctaaggatt ttatatcagg attaatgatt aatttaggtg cattgagtgg ttctcgctta
     3601 agttgaacat cacgcattaa ttctagttga tcttcataaa tatgcgcatt gacaatttta
     3661 tggaacgctt gtcctgcctt atgccctgta atttgagcca ttaaagctaa aaaggtatag
     3721 cattgaatca tattccaatt taacccaagc ggtacatctg ccgaacgctg agtactattc
     3781 aggtaaagcg tgccatcaag tagagaaaaa tgatggctgt gtaaacaagg acgtaagcag
     3841 cccatatgga aagcgccggg gtggtagaag gtataaattt caccacggtt atcaattcct
     3901 tttttcaaat cgtccacaat ttgtcgcaat aaatcgactg agccaccgtc tggtttcggg
     3961 aaattacgcc ctactgcgcc gtaaaccaag cccatatcat cttccccttt tcgataggga
     4021 ttagcaagcc attgtgcatt ttcatttgca ttagcgtccc acgattttgt gcctaattga
     4081 cggaaatcag cagcattact atagcctcga atatagccta aaagttcggc aatcgcagct
     4141 ttccaataac tacggcgagt ggtaactaaa ggaaattctc ctttttctac gtcataagtt
     4201 aagtcagcat taatgactgt gaggcagtgt ttgcctgtac gttcatttgc aacccatttg
     4261 ccttcgctaa cgatgcggtt acataagtct aaatactgtt tcattattta cctcataaaa
     4321 aattttaggt aattttgcca tttattcggt aaaatagcta aaactttatg acacaacaca
     4381 ataaggagtt atggatatga aaggtataat gaaatccatt ctgggttttt ccaccgctgt
     4441 ttttttagca gcttgttcat cgaataaaag cggttggaca cgtttaaatg ataatgatgt
     4501 tgatcagaaa tcttatgcta ttgcttatgg tgcaacggct cagacttatg cggatagagt
     4561 aaatgaaagc tatgatatca attcctttat gaatggggta aatgattggt acactaaaaa
     4621 ggttacattg ccaattgagc aaattagagc aataacgatt aatcgaatgt tagaccataa
     4681 tgtgtatgct tattatagtg gtgtgattta tgccgcagat ttgcaggcaa atttcaatca
     4741 tttagatcct aattgttggg ggcttgttca accagcgagt atgacccagg gcattcacga
     4801 tgcgatgtta gatttacaga agaacaaagt tcgtggagat gagtatatca aaaatggggt
     4861 tgagcaaata ttgcaccttt gtgtaaaaac gattgctgaa gatgaaaata aatcgcaatc
     4921 taaaggtaaa accaaaaagc ataaataagt taagctatga caagcggtaa aatttttaaa
     4981 aaattttacc gcttttttat aaccgtatga aatgtttaat atttattcta tgaaacttaa
     5041 aaaaacagcg ttttgtttat tcaataggca aacactcggg atttttcaat tttcaggaaa
     5101 aagaatttga catcttttta aattctagta atatggcgcc cacagacaca gtgagtggtg
     5161 agatggccga gcaggctgaa ggcgctcccc tgctaaggga gtatagggtt aaaaagctct
     5221 atcgagggtt cgaatccctc tctcaccgcc atttacttta tcacgcaccc gtagctcagc
     5281 tggatagagt actcggctac gaaccgagcg gtcagaggtt cgaatcctct cgggtgcgcc
     5341 atttctatat tatatccatt atattcccat tttatttttt taataaataa ttaaactata
     5401 aagtattatt ccgttaattt gtaatttcag ataggcttaa tgcttagctt gaaataatga
     5461 ggattttaaa ttgaaaggat taattgctcg cctttttatt gccgtagcat tgttactgtg
     5521 ggcttgggat atgatttttc cttggcaaaa aatcattcat tcagaagcaa atcattatac
     5581 acaaattcag caaagcaaaa cgttaaaggt tggaatgatc aaccatccac tttcttactt
     5641 tgttggtcct gaaggaaaaa gtggcattga atatgattta gcaacagcct ttgctcaata
     5701 tttaaaggtc aatcttgata ttaaaactta tgatagtagc gagaaattgt ttgaggcctt
     5761 aagaaataat gatgttgata ttgctgccgc tgggctgctt taccattctg aattaaacga
     5821 tgaatttcag ataggtcctt cctattattc ggcttcttgg cagcttgttt ataaaaaggg
     5881 aagtgctaga ccatatcgac tttccgattt gcgggaaaat gtgattattc cagccggttc
     5941 tgcggtaatt ccaatattaa atcagcttaa ggtggataat ccggaactaa aatggcagat
     6001 ctcggataaa tttacacaag aagagctatt gttaaaagtc gcacaaggtg aaatttcaca
     6061 tacaattgca atgtcggttg atatttctgc tgcacagcat attaatccta atttagccgt
     6121 agggtttgat ttaactgatg aaaacccagt gttatggtat ttagcaaata gttcttatag
     6181 tgaactgcaa tctgtgctgc tggattttat gtcttattct atggaaacgg ggctgatatc
     6241 aagaatagaa gagaaatatt ttaatcattt agctaagttt gattatgttg atattcagag
     6301 ctatttgaat gcaattaaaa ctattttacc taaatatgaa cccctttttc gggcacataa
     6361 aggtgaatta gaatggcaaa tgctggcggc tatcgcctat caggagtctc attgggatcc
     6421 taatgcaact tctccaaccg gagtgagggg aattatgatg ttgacaaaag atacggcaga
     6481 tagaatgaaa atttctgata ggactaaccc tgcacaaagt atcaaagcgg gatctgaata
     6541 tttacatatg ctaatgagac aaatgccgga aactattcct caagcagata gaatttggta
     6601 tagcctagca gcgtataata tgggattggg gcacttgttg gatcttcgtc gattaacaaa
     6661 acaacttggt ggtgatccgg ataattggct tgatgttaaa aagaatctgc cattgcttgc
     6721 ggaaaaacgt tattatgcta atctaaaata cggctatgct cgaggatatg aagcattaca
     6781 gtatgttgaa aatatacggc gttattatgg tagtattatt aattatcaac gagttgaaga
     6841 gcaaaagatg caagaacaag aaaatttacc agatggtcaa ataaaacagg agaattcttc
     6901 ttatgagaaa attgaagaaa aagaaatccc taccgacaag gaaattacgc ctgcgtcaaa
     6961 ttaagcaaat taaagataga aaaagattaa tttctgcacg tagaaaggca tttatgcaga
     7021 tgcaacaggc catctg
//
LOCUS       DANJWC010000123         6893 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.123, whole genome shotgun sequence.
ACCESSION   DANJWC010000123 DANJWC010000000
VERSION     DANJWC010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6893
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.123"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..815)
                     /gene="metC"
                     /locus_tag="PWX02_001848"
     CDS             complement(<1..815)
                     /gene="metC"
                     /locus_tag="PWX02_001848"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HDL1729835.1"
                     /translation="MSNYKNLSTKLVHTGRKKRNTQGAVNPVIQRASSLVFETLEEKK
                     HATQNRYKGALFYGRRGTLTHFSLQEAMCELEGGAGCYLYPCGAAAVTNAILAFVEQG
                     DHILMTGAAYEPTQEFCNVLLKRMGVSTTYYDPMIGEGLKALVQPNTKVLFLESPSSL
                     TMEVPDIPTLVRVAREINPEIVIMIDNTWSAGVLFPALEYGIDISIQAGTKYLVGHSD
                     VMIGTAVSNARCWDKLREGSYLMGQMVDADSAYITARGLRTLGIRLKQHEQSS"
     gene            complement(874..1413)
                     /gene="dsbB"
                     /locus_tag="PWX02_001849"
     CDS             complement(874..1413)
                     /gene="dsbB"
                     /locus_tag="PWX02_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HDL1729836.1"
                     /translation="MLYFFKNLSQSRSAWFLLMLSCLILEGTALYFQHGMGLVPCVMC
                     IYERVALFGILFAGILGFLGNRYAIIRYLAILIWLGSAFKGLILSIKHHDYQVNPSPW
                     NQCEFKVNFPQTLPLDQWLPQVFAPGPVNCSQSQWEMFGFSMAQWLIFTFAVYLIVAV
                     IVLISQFKRSRQRNNFMFR"
     gene            complement(1476..3017)
                     /gene="nhaB"
                     /locus_tag="PWX02_001850"
     CDS             complement(1476..3017)
                     /gene="nhaB"
                     /locus_tag="PWX02_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton antiporter NhaB"
                     /protein_id="HDL1729837.1"
                     /translation="MSSIDALFKSFLGKSPEWYKLCILAFLIINPLIYFFINPFVAGW
                     FLVAEFIFTLSMALKCYPLQPGGLLAIEAVVIGMTNPHHIKEEIMANFDVILLLMFMV
                     AGIYFMKQLLLYIFTKLLVAIHSKKILSLSFCLSAAFLSAFLDALTVIAVIISVGTGF
                     YGVYHKVASGNNFEDSTDITNDEKIISNKEILEQFRGFLRSLLMHAGIGSALGGVMTM
                     VGEPQNLIIASQAEWGFIEFLLRVLPVSLPVLICGIATCFLVEKYKLFGYGDRLPSKV
                     WVVLARYSQLKEQKMTKQDRLKMLVQAIAGIWLIIGLALHLADVGIIGLTIIIICTAF
                     CGITDEHTLGKAFQESMPFTALLIVFFSVVAVIIDLKLFEPIIAFVLSAEPHSQLSLF
                     YIFNGLLSMISDNVFVGTVYINEAKTALDAGIIDREQFDLIAVAINTGTNLPSVATPN
                     GQAAFLFLLTSSFAPLIKLSYGRMVYMALPYTIVLSIVGFLGLEFLLPSMTEVMINWG
                     WFITR"
     gene            3174..3899
                     /gene="fadR"
                     /locus_tag="PWX02_001851"
     CDS             3174..3899
                     /gene="fadR"
                     /locus_tag="PWX02_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HDL1729838.1"
                     /translation="MGHSDILKAQSPAALAEEYIVKSIWNNHFPAGSDLPAERELAER
                     IGVTRTTLREVLQRLAREGWLSIQHGKPTRVNDIWETSGPNIIGTIIKLDKSYLPVVI
                     ANVVSLRTRMAEAYIPEAIYLAPEESAKLFDGLDELENTAAAYAEFDYKLFRQFTFLA
                     NKPVYALILNSFKSMYHQVAKIFFADENNRQLTLSFYRSLLCTCLAKDSEKAAACMAK
                     NRQSSGEIWQKLSANIPESFGQL"
     gene            3914..4984
                     /locus_tag="PWX02_001852"
     CDS             3914..4984
                     /locus_tag="PWX02_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDL1729839.1"
                     /translation="MNEIIFSLEPQDNARLQSLCGAFDEHLALIEKSFNLIIARTGFN
                     FTIQSEDDNHYSATLVQNSVKLIKQLYNETAPIKGKIKELDLEDIHLAIQESRMLLQN
                     QWQAGSQGEIAIKTKRGVIKPRGEHQQAYLRNILSHDISFGIGPAGTGKTFLAVAAAV
                     ESLERQEIRRILLTRPAVEAGEKLGFLPGDLGQKIEPYLRPLYDALFEMLGFEKAQKL
                     MERGVIEIAPLAYMRGRTLNDAFIILDESQNTTTEQMKMFLTRIGFNSKAVITGDITQ
                     VDLPRSQKSGLKHAMEVLKEVPELSFNFFDSKDIVRHPVVAKIVQAYDAWEAVDEERK
                     EQRKLEKLALEQEKILAQAEML"
     gene            complement(5107..>6893)
                     /gene="tkt"
                     /locus_tag="PWX02_001853"
     CDS             complement(5107..>6893)
                     /gene="tkt"
                     /locus_tag="PWX02_001853"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDL1729840.1"
                     /translation="LHLTGYDLSIEDLKQFRQLHSKTPGHPEYGYAPGVETTTGPLGQ
                     GITNAVGIAIAEKTLAAQFNREGHKIVDHYTYAFLGDGCLMEGISHEACSLAGTLGLG
                     KLIAFYDDNNISIDGHVDGWFTDDTAQRFESYGWQVIRNVDGHNPEQIKFAIENAQAE
                     TERPTLIICKTIIGYGSPNKSNSHDCHGAPLGDAEIQAAREFLGWEHAPFVIPAEIYT
                     EWDAKAKGTLAEKDWNAKFAAYEAAYPELAAEFKRRYAGELPANWEAESKAFIEKLQA
                     NPATIASRKASQNAIEAYAPILPEFLGGSADLASSNLTLWSGSKPIRAEHNVDGNYIN
                     YGVREFGMSAIMNGIALHGGFIPYGATFLMFYEYAHNAVRMAALMKQRALFVYTHDSI
                     GLGEDGPTHQPVEQTASLRQIPNLETWRPADQVESAVAWKAAVERKDGPTALIFTRQN
                     LAQQQRTAEQLANISRGGYILRECCEKGSCPDLILIATGSEVDLAMKAADVLDAEGVK
                     VRVVSMPSTNVFDKQDEAYRESVLPRSVTKRVAIEAQLADFWYKYVGFDGRIVGMNSF
                     GESAPADQLFKLFGFTVENVVAKAKEIL"
BASE COUNT         2142 a         1371 c         1429 g         1951 t
ORIGIN      
        1 atgctacttt gttcgtgctg ttttaagcga atgcctaagg tgcgtagccc ccgagcggtg
       61 atgtaagcgg aatcagcatc caccatttgc cccattaaat aagagccttc acgcagttta
      121 tcccaacaac gagcattgga aacggctgtg ccgatcatca catcagaatg cccgactaaa
      181 tatttagtgc cggcttgaat ggaaatatca atgccatatt ctaaagcagg gaacagcaca
      241 ccggcagacc aagtgttgtc gatcataatc acgatttctg ggttgatttc acgagcaaca
      301 cgcacgagag tcggaatatc aggcacttcc attgttagcg agctcggcga ttctaaaaaa
      361 agcactttgg tgttcggttg cacaagggct ttcaagcctt caccaatcat cgggtcataa
      421 taagtagttg aaacccccat acgttttaac agcacattac agaattcttg tgtcggttcg
      481 taagctgcgc ctgtcattaa aatgtggtca ccttgttcca caaaagctaa aatcgcattg
      541 gtgactgccg ccgcaccgca agggtaaagg tagcaacctg ctccaccttc taattcacac
      601 atcgcttctt gtaatgaaaa atgagtgaga gtgccacgtc tgccataaaa taatgctcct
      661 ttgtagcggt tttgtgtagc gtgttttttc tcttctaagg tttcaaacac taaagatgaa
      721 gcacgctgaa tcaccgggtt aactgctcct tgtgtgttac gttttttacg ccctgtatga
      781 acaagtttgg tggaaagatt tttatagtta gacataactt ttcctaaagt aaaatgcata
      841 ggggcaaatc aacatttgcc cctttattaa taattaacga aacataaagt tatttctttg
      901 acggctacgt ttaaattggc tgattagcac aatgaccgca acaattaagt aaaccgcaaa
      961 agtaaagatt agccattgtg ccattgaaaa gccgaacatt tcccattggc tttggctaca
     1021 attaaccgga ccgggggcaa acacttgtgg tagccattgg tcaagcggca aggtttgcgg
     1081 gaaattaact ttaaattcgc attgattcca tggtgatgga ttgacctgat aatcgtggtg
     1141 tttgattgaa agtattagcc ctttaaatgc gctaccaagc caaattagaa ttgccaagta
     1201 gcgaataatc gcatatctat taccaagaaa tcctaaaata ccggcaaata aaataccaaa
     1261 taaagcaact cgctcataaa tacacattac acaaggtact aatcccatcc catgttggaa
     1321 ataaagcgct gtgccttcaa gtataagaca gcttaacatt agtaaaaacc atgcactgcg
     1381 agattgtgat agatttttaa aaaaatagag catagttatt cctcattttg tgaacttaag
     1441 gcattctacc ttatttggta tgagttatct ataaactaac gtgtaataaa ccatccccaa
     1501 tttatcatta cttcagtcat agaaggtagt aagaattcta accctaagaa accgacaata
     1561 gatagaacaa tggtataagg taatgccata tagaccattc tgccgtaaga cagtttaatt
     1621 aacggcgcaa aggaggacgt taataagaat aagaaagcag cttgtccgtt tggggtagca
     1681 acagaaggaa gatttgtgcc agtattaatt gcaactgcaa tcaggtcgaa ctgctcacga
     1741 tctataatgc cggcatctag tgcggtttta gcttcattaa tgtaaacagt accgacgaag
     1801 acgttatctg aaatcatcga gagcaaacca ttgaaaatat agaataatga aagctgtgaa
     1861 tgtggttcgg cagataacac aaaggcaatg atcggctcaa ataattttaa atcaataatc
     1921 acggcaacaa ccgagaaaaa cacaataagc aatgcagtaa aaggcataga ttcttggaat
     1981 gctttcccta aagtgtgttc atcagtaatg ccacaaaatg cagtgcaaat aatgatgatg
     2041 gttaagccaa taatccctac atcggcaaga tgtagcgcta agccaataat tagccaaatt
     2101 ccagcaatgg cttgcaccag catctttaag cgatcttgtt tcgtcatttt ttgttctttt
     2161 agttggctat agcgagcgag tacgacccaa actttgctag gaagcctgtc tccataacca
     2221 aataatttat atttttctac aagaaagcag gtcgcaatcc cacaaattaa cacgggtaag
     2281 ctaactggta atactctaag taagaattcg ataaatcccc attccgcttg ggaggcaata
     2341 attaagtttt gtggttctcc caccatggtc attacgccac ctaaagctga gccaataccg
     2401 gcgtgcatca ataaacttct taaaaaacca cgaaattgct ctaaaatttc tttattagag
     2461 atgatttttt catcattggt aatatcagta gaatcttcaa agttattacc tgatgcgact
     2521 ttatgatata cgccgtagaa acctgttcca acgctaatga ttacagcaat aaccgttaaa
     2581 gcatcaagaa aggcggataa aaaagcagcg cttaagcaga aagaaaggga aagaattttt
     2641 ttcgaatgaa tggcgacaag cagtttggtg aaaatataaa gcaataattg cttcataaag
     2701 taaataccgg caaccataaa cattaaaaga agaataacat caaagtttgc cataatttct
     2761 tctttaatgt ggtgaggatt tgtcataccg attacaactg cttcaatcgc taataaacca
     2821 cctggctgta atggataaca ttttaatgcc atagagagag taaaaataaa ttcagcgact
     2881 agaaaccagc ctgcaacaaa aggattgata aagaaataaa tgagaggatt gataattaaa
     2941 aatgccaaaa tgcacaattt ataccactcg gggctttttc ctaagaaact tttgaacaga
     3001 gcatcaatgc tactcatata aaggacctca aaatttgtaa acagatatta caattgtaat
     3061 atagtttaga ttaaaggata atagtattac tgaacttttt agtcgcaata attgattaga
     3121 aataaagaat agttagattt tagataagca tattttttgc ttggggggca ttaatgggtc
     3181 acagtgatat tttaaaagcg caaagcccgg cagcgttagc ggaagaatat attgtaaaga
     3241 gtatttggaa taatcatttc cctgccggct ctgatcttcc tgccgagcga gagttagcag
     3301 agcgcattgg ggtaacaaga acaactttac gtgaagtact acaacgtcta gcgcgagaag
     3361 gttggttaag tattcagcac ggcaagccaa caagagtgaa tgatatttgg gaaacttcgg
     3421 ggcctaatat tattggcact attattaagc tagataaaag ctatcttcca gtggtgattg
     3481 ccaatgtggt gtcattacgt actcgtatgg cggaggctta tattccagaa gcgatttacc
     3541 ttgctcctga agaaagtgct aaactctttg atggactaga tgaattagaa aatacagcag
     3601 ctgcttatgc tgaatttgat tataagttat tcagacaatt tactttttta gccaataagc
     3661 ctgtgtatgc gttgatccta aatagcttta aatcaatgta tcatcaagtc gcaaaaattt
     3721 tctttgctga tgaaaataat cgtcaattaa cgttaagctt ttaccgttca ttattatgta
     3781 cttgtcttgc aaaagatagt gaaaaagcag ccgcttgtat ggctaaaaat cgccaatcta
     3841 gtggtgaaat ttggcaaaaa ttatccgcta atattcctga aagctttggt cagctttaac
     3901 agaaggtaga attttgaacg aaattatttt tagcttagaa ccgcaagata atgctcgtct
     3961 gcaatcgctt tgtggtgcat ttgatgagca tttagcctta attgaaaaaa gttttaatct
     4021 cattattgct cgaacaggct tcaactttac gattcaatcg gaagatgaca atcactattc
     4081 tgctaccttg gttcaaaata gtgtgaagct gattaagcaa ctgtataatg aaactgcccc
     4141 gattaaaggc aaaattaagg aattggactt agaagatata catttggcga ttcaggaaag
     4201 ccgaatgcta ctacaaaacc aatggcaagc cggctcgcaa ggcgaaatag cgattaaaac
     4261 caagcgaggg gtgattaaac ctcgtggcga acatcaacaa gcctatctgc ggaatatatt
     4321 aagccacgac atcagttttg gtatcgggcc tgctggcaca ggcaaaacct ttttagcggt
     4381 agcggcagcg gtggaatcgc ttgaacgcca agaaatccgc cgtattttac ttacccgtcc
     4441 ggcggtggaa gccggtgaaa agctcggttt tttacccggc gatttagggc aaaaaattga
     4501 gccttactta cgcccgttat atgatgccct gtttgaaatg cttggctttg aaaaagctca
     4561 aaagttgatg gaacgtggtg tgattgaaat tgccccactt gcttatatgc gagggcgtac
     4621 tttaaacgat gcgttcatca ttttagatga aagccaaaac acgactaccg agcaaatgaa
     4681 aatgttctta acccgtattg gctttaattc caaagcggtg attaccggcg atattaccca
     4741 agtggatttg ccccgtagcc aaaaatcagg cttaaaacac gcaatggaag tgctaaaaga
     4801 agtgccggaa ctcagcttta atttctttga cagcaaagat attgtccgcc accctgtggt
     4861 tgccaaaatt gtgcaagcct atgatgcgtg ggaagcggtt gatgaagagc gtaaagaaca
     4921 gcgaaaactg gaaaaattag ctttagagca agaaaaaatt cttgctcaag ctgaaatgtt
     4981 atagcttaca agcggtcata tttcgctaat tttttgcaaa aaatcgctga aatttcaccg
     5041 cttatcatag ttgttcaaca aaaaagcggg aagttaattc ccgcttttct ttatcttatt
     5101 gtttcattat aagatttctt ttgctttagc aaccacgttt tcaacggtaa agccgaagag
     5161 tttgaataat tgatctgctg gtgcggattc accaaagcta ttcataccta caattcgacc
     5221 atcaaagccg acatatttat accagaagtc agctaattga gcttcaatcg ccacacgttt
     5281 agtgactgaa cgtggtaata ccgattcacg ataggcttca tcttgtttgt caaacacgtt
     5341 ggtgcttggc attgaaacta cacgcacttt cacgccttct gcatctaaca catcagcggc
     5401 tttcattgct aaatctactt cagaaccggt tgcaattaag attaaatccg ggcaactgcc
     5461 tttttcgcaa cattcacgca agatatagcc accacgagag atgttcgcta attgctccgc
     5521 agtacgttgt tgttgagcca gattttggcg ggtaaagatt agtgctgtcg ggccgtcttt
     5581 acgttctacc gccgctttcc aagcaacagc agattctact tggtctgccg gacgccaggt
     5641 ttctaagttt ggaatttggc gtaatgaagc agtttgttct accggttggt gggtcgggcc
     5701 gtcttcacct aaaccgatgg agtcgtgtgt ataaacaaat aaggcacgtt gcttcattaa
     5761 ggctgccata cgcaccgcat tgtgtgcata ttcgtagaac attaagaagg ttgcaccgta
     5821 aggaatgaaa ccaccgtgta gggcaatccc gttcataatc gcagacatac cgaattcacg
     5881 cacgccgtag ttgatgtagt taccatctac attgtgttcc gcacgaatcg gttttgagcc
     5941 agaccataag gttaagttag agcttgctaa gtcagctgag ccgccgagga attcaggtaa
     6001 gattggcgcg taagcttcga ttgcattttg tgatgcttta cggcttgcaa tggttgccgg
     6061 atttgcttgt aatttttcaa taaaagcttt gctttcagct tcccagttag ccggtaattc
     6121 gcctgcgtaa cggcgtttaa attcagcggc tagttcaggg tatgccgctt cataagcagc
     6181 aaatttagcg ttccaatctt tttctgctaa tgtacctttt gcttttgcat cccactctgt
     6241 ataaatttca gctggaatca caaatggggc atgttcccaa cctaaaaatt cacgagcggc
     6301 ttggatttca gcatcaccca atggcgcacc gtggcaatcg tgagagtttg atttatttgg
     6361 tgaaccatag ccgataatgg ttttacaaat aattaaagtt ggacgctcgg tttcggcttg
     6421 tgcattttca atcgcaaatt tgatttgttc tgggttatgg ccatcgacat tacggataac
     6481 ttgccagcca taagactcaa agcgttgtgc ggtatcatca gtgaaccaac catcaacgtg
     6541 gccgtcgata gagatattgt tatcatcgta gaaagcaatc agtttgccta agcctaatgt
     6601 accggcgaga gagcaagctt cgtgtgagat accttccatt aaacagccat cgcctaagaa
     6661 agcgtaagtg tagtgatcga caattttatg accttcacgg ttaaattgtg ctgcaagggt
     6721 tttttccgca atcgctatac cgaccgcatt ggtaatacct tgacctaacg ggcctgtggt
     6781 ggtttcaaca cccggtgcat aaccatattc agggtgacca ggtgttttag aatgtaactg
     6841 acggaactgt tttaaatctt cgatagagag atcatagccg gttaaatgaa gta
//
LOCUS       DANJWC010000124         6860 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.124, whole genome shotgun sequence.
ACCESSION   DANJWC010000124 DANJWC010000000
VERSION     DANJWC010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6860
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.124"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..591)
                     /locus_tag="PWX02_001854"
     CDS             complement(<1..591)
                     /locus_tag="PWX02_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005567524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1729841.1"
                     /translation="MITVKNLHHKIGNATILNNINLTIPNGGITALIGANGAGKSTLL
                     SLIARLQPIQQGEIHLNELNTANTASQTIARQLAILTQDNVIHSRITVKDLLLFGRYP
                     HHQGRITSHDNAIVEEALVRFDLSTFSHRFLNELSGGQRQRALIAMTFCQQTDHILLD
                     EPLNNLDMFHARELMKLLRKLTDELALTTIMVVHDIN"
     gene            complement(611..1489)
                     /locus_tag="PWX02_001855"
     CDS             complement(611..1489)
                     /locus_tag="PWX02_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL1729842.1"
                     /translation="MTYNAKGNWDFILQLRGKKLILLCTIAYTIGISTLLFQTLTHNP
                     ILTPSILGFDSLYLLLQTSLVFLFGGLGFTQLDPSYKFFFETLLMLGASILLFSSLNR
                     NRSDLTRMLLIGVIFGVLFRSLNNLLQRMIDPTEFAVAQGTSFASFNLTTPTLLWIGG
                     CIAILSAIWVWLQRHKLDILLLGKDKAIGLGLEYHQFSRQLLICCGLLVSVSTALVGP
                     ILFLGLLVCAIVNAISPTVCHSVRIPMTFLISAITLILGQAIFEQVLKMQGVLSVVIE
                     FIGGIVFIYLILKRKK"
     gene            complement(1542..2510)
                     /locus_tag="PWX02_001856"
     CDS             complement(1542..2510)
                     /locus_tag="PWX02_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL1729843.1"
                     /translation="MFSFFRLNLFLLILLIPISASVGVADFHWSEIFHSSEQSQIFFI
                     SRLPRTLAVILVGATLAVAGMVLQIVLKNRFIEPSMIGTSQSAAIGILLASLLFPTSA
                     LLLKMSIATLSALMGMGLFMLLIRQLPPHQQLMLPLIGIVFGNVIEAISTFIAYETDS
                     LQVLSIWFSGDFSGILAGRYELLWITAILAVVVYVMADQLTIAGLGENISTNLGINYK
                     QMTWLALIVVAMITAIVVVTVGQIPFIGLVVPNIVSRIAGDRLRKNLPAVVVLGANLV
                     LICDILGRAINAPYEVPISTIFGIIGTVIFLYLLLKGKRAEEKARG"
     gene            complement(2604..3374)
                     /locus_tag="PWX02_001857"
     CDS             complement(2604..3374)
                     /locus_tag="PWX02_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL1729844.1"
                     /translation="MFDTGSLDTLQALGVKIDGMPEFKVLSYLKPSADKAVNVGTVFE
                     PDLEALNSLNPDLIIVGTRTAKTFDEVSQIAKTIDMTDNGDKLIESGLQRLDSFGKLF
                     NKEAETEKLKAEINTLLDQTKVAVKGKGNGLIILVNGGKISAFGSGYRLSWIHDNLGI
                     PMADSSIKNTGHGQPVSFEFIQKLNPDWLFVLDRIAAIGQEGQSAQQVLDNELVRQTK
                     AWKNGNIVYLSAASYLAPGGYEQMKTDLTNIKTAFEKK"
     gene            complement(3335..3499)
                     /locus_tag="PWX02_001858"
     CDS             complement(3335..3499)
                     /locus_tag="PWX02_001858"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729845.1"
                     /translation="MIKKALFALTATAVFAASAFAKDVTIPTAERLLSKKPRLKLPCL
                     TPEVWILYKP"
     gene            3673..5136
                     /locus_tag="PWX02_001859"
     CDS             3673..5136
                     /locus_tag="PWX02_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL1729846.1"
                     /translation="MAEYSGTTQEKMKDKQVEQINISPHSIEAEQAVLGGIMLSNDHW
                     DNVAERVQPHDFYNYAHRIIFEQMAELVRHNKPIDIITLDQALKDKGVLQDVGGFAYL
                     AELSKNTPSAANILAYADIVRDRSDLRSVISAGNQIAEMGYKTKGRNSKEVLDEAERI
                     VFEIAEKRNNANEGPQRIDDILLKTLARMDSLSKNRHNGGVTGVSTGFVDLNKKTAGL
                     QPSDLIIVAARPSMGKTTFAMNLCENASLVDIEDKDDLDEQGNPKKKPAKPVLIFSLE
                     MPADQIMMRMLASLSRVDQTKIRTGQISDDEDTAKISSTMAILQERNNIYIDDSSGLT
                     PTELRSRARRIYRENGGLSLIMVDYLQLMRAPGFADNRTLEIAEISRSLKALAKELEV
                     PVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYRDEVYNDNSDLKGIAE
                     IIIGKQRNGPIGRVRLTFQGQYSRFDNYTGGHYDDEY"
     gene            5126..5431
                     /locus_tag="PWX02_001860"
     CDS             5126..5431
                     /locus_tag="PWX02_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HDL1729847.1"
                     /translation="MNTKPTQPITLCAIYRSKAKEGMYLYVPKRDQFEQVPETLRQLF
                     GKPEFVMMFNLTGEKPLVRAKNEEVLQLLIEQGYYLQMPPPPENLHKAFIAQQKGEK"
     gene            5431..5880
                     /gene="nrdR"
                     /locus_tag="PWX02_001861"
     CDS             5431..5880
                     /gene="nrdR"
                     /locus_tag="PWX02_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HDL1729848.1"
                     /translation="MRCPFCSTEETKVIDSRLAASGYQIRRRRECISCKERFTTFESA
                     ELVVPYVIKNNGSREPFDAEKLRTGLRRALNKRPVSADDVEKAISQIIIQLQAMGERE
                     VPSRHIGTLAMNALKQLDKVAYIRFASIYLSFSDIEEFIKEIEKLKE"
     gene            complement(5954..6823)
                     /locus_tag="PWX02_001862"
                     /pseudo
     CDS             complement(5954..6823)
                     /locus_tag="PWX02_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991647.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         2172 a         1386 c         1419 g         1883 t
ORIGIN      
        1 gttgatgtcg tgtaccacca ttatagtagt taaggcgagt tcatcggtta attttcgtaa
       61 taatttcatt aattcccgag catggaacat atctaaatta ttcagtggtt catctaataa
      121 gatatgatcg gtttgctgac agaaggtcat tgcaattaat gcccgctggc gttgccctcc
      181 ggataattca tttaagaatc ggtgggagaa agtcgataaa tcaaatcgta ccagagcctc
      241 ttctacaatg gcattatcgt gcgaagtgat tctgccttga tgatgagggt aacgcccaaa
      301 taacagcaga tctttgacgg taatacggct atgaatcaca ttatcttggg tcaagatagc
      361 cagttgtcga gcgatagttt gagaagctgt gttggctgta ttcagctcat ttaagtggat
      421 ttcaccctgt tggataggtt gcaagcgtgc aattaaagag agcagggtgg atttgcccgc
      481 accatttgct ccgattaaag cggtaatacc accatttgga atggttaaat tgatattatt
      541 cagaatcgtg gcattcccga ttttgtggtg aagattttta accgttatca ttttcttcct
      601 taatttatgg ttattttttc cgttttaaaa tgagataaat aaagacaata ccgccaataa
      661 attcaataac gacactcaga accccttgca ttttcagcac ttgctcgaag attgcctgcc
      721 ctaaaattaa ggtgatggca gagattaaaa aagtcattgg tattcgcacg ctatggcaaa
      781 cagtcgggct aattgcatta acaatggcac aaacaagtag ccctagaaat agaatcgggc
      841 caacaagtgc ggttgaaaca gacaccaaca atccacaaca gattagtaat tgacgtgaaa
      901 attgatgata ttctaaccca agcccaattg ccttatcttt gccaagtaac aaaatatcta
      961 atttatgccg ttgtaaccag acccaaatgg cagagagaat agcgatacag cctccaatcc
     1021 aaagtaatgt aggggtagtc aggttaaatg aggcaaaaga agtgccttga gcaacggcaa
     1081 actctgttgg gtcgatcatt cgttgcaaca aattatttag gcttctaaat aagacaccaa
     1141 agatgacacc gattaaaagc attcgggtta aatcggatcg atttcgattc agtgaactaa
     1201 atagtaatat tgatgcccct agcattaaca aggtttcaaa gaaaaactta tagcttgggt
     1261 ctaactgagt aaagcctaaa ccgccaaata aaaatactaa cgaggtttgc aatagcaagt
     1321 agagcgaatc gaaccccaaa atacttggag ttaaaatagg attatgtgtg agtgtttgga
     1381 atagcagggt cgaaatacca atcgtataag caatagtaca gagcaaaata agctttttgc
     1441 cacgcagttg caatataaag tcccaattac ctttcgcatt ataggtcata taaaacagaa
     1501 tacttgctaa taagattaag cttaaaacga taaagcgtga tctaaccacg agccttctcc
     1561 tcagcccgtt tccctttgag caataaatag aggaaaatca ctgtcccaat aatcccaaaa
     1621 atagtagaaa tcggcacttc ataaggggca ttgatggctc gccctaaaat gtcacaaatc
     1681 aggactaaat tcgcacctaa caccacgacc gcaggcagat ttttacgcaa tcggtcacct
     1741 gctatgcgag agacaatatt agggacgacc aagccgataa acggaatctg tccaaccgtc
     1801 accacgacaa tagcggtaat catcgccacc acaattaagg caagccaagt catttgtttg
     1861 tagttaatgc ctaagttggt actaatattt tcaccaaggc ctgcaatggt taattgatct
     1921 gccatcacat aaaccacaac agccagaata gcggtaatcc agagcagttc ataccgacct
     1981 gccaaaatac cggagaaatc gccggaaaac caaatcgaaa gcacttgtag gctgtcagtt
     2041 tcataagcaa taaaagtgct gatagcttca ataacattgc caaaaacgat cccgattaat
     2101 gggagcatta attgttgatg aggcggtaat tgacgaatga gcaacataaa aagccccatt
     2161 cccattaatg cacttaatgt ggcaatagac atttttaaca gtaatgctga tgttgggaaa
     2221 agtaaactag caagtaaaat accaattgcc gcactttggc tagtgccaat catactaggc
     2281 tcaataaagc ggttcttcag cacaatttgt aataccatac ctgcaacggc taaggttgca
     2341 ccgactaata tcactgcgag cgttcttggt aaacggctga taaaaaagat ctgactttgt
     2401 tcggaagaat gaaaaatttc actccaatga aaatctgcca cacccacact ggcactgatt
     2461 ggaatgagca atattaaaag aaatagattt aatctaaaaa aagagaacat agtgataaat
     2521 agcaagcggt catattctct ctctttttta caaatgcagg cacttgcaaa aataggagga
     2581 aatctgaccg cttgtaaggt taattatttt ttctcaaatg ctgttttaat gttagttagg
     2641 tcggttttca tctgctcata accacccggc gctaaatatg aggcagcact tagataaacg
     2701 atattaccgt ttttccaagc tttagtttga cgaactaatt cattatccaa cacttgttgg
     2761 gctgattgac cttcttgtcc gatagccgca atacggtcta acacaaacaa ccaatccgga
     2821 ttgagttttt ggataaattc aaacgaaaca ggctgaccgt gaccggtatt tttaatgcta
     2881 gaatctgcca ttggaatccc taaattatcg tgaatccaac ttaaacgata acctgaacca
     2941 aatgcagaaa ttttgccacc attgactaaa ataatcagtc cgttaccttt accttttact
     3001 gccactttgg tttggtctaa taaagtatta atttctgctt ttagtttttc ggtttccgct
     3061 tctttattaa ataatttgcc gaaagaatca agacgctgta atccgctttc gattaattta
     3121 tcgccattat ccgtcatgtc gatggttttc gcaatttggc tgacttcatc aaatgttttt
     3181 gctgtgcgtg tgccaacaat gatcaaatcc ggatttaaag aatttaacgc ttctaaatcc
     3241 ggctcaaata ccgtaccgac attcaccgct ttgtctgcac tcggtttaag ataagaaagc
     3301 accttaaatt caggcatacc atcaattttt acacctaagg cttgtaaagt atccaaactt
     3361 ccggtgtcaa acacggcaat tttagacggg gtttctttga aagtaacctc tctgcagttg
     3421 gaatagtaac atctttggca aatgcagaag cagcaaacac agcagttgcg gtcaatgcaa
     3481 acaaggcttt tttaatcatt ggataactcc atttatattg aaaataattc tcaaagattt
     3541 taacgaaaat tgattttaag taaagcggaa aataacaagc gggcgaattt tgcctaaaat
     3601 ttgcaaattt caatttaatt aaaattccga tacactatgt acatcaatac gtcattatag
     3661 caagagaaaa atatggcaga atatagcgga acaacccaag agaagatgaa agataagcag
     3721 gttgagcaaa ttaatatctc accacattcg attgaggcag agcaagcggt gcttggtggc
     3781 attatgctca gtaatgatca ctgggataat gttgctgagc gtgtacaacc gcacgatttt
     3841 tacaactatg cgcatcgaat tatttttgaa caaatggcgg agttggtgcg ccacaataaa
     3901 ccgattgata ttattacgct ggatcaagcc ttaaaagaca aaggggtgct gcaagatgta
     3961 ggaggttttg cctatttagc cgagctttct aaaaataccc cgagtgcagc aaatatttta
     4021 gcctatgcgg atattgtgcg agatcgttcc gatttaagaa gtgtaatcag tgccggcaat
     4081 caaattgcgg aaatgggcta caaaacgaaa gggcgtaact ccaaggaagt gttagacgaa
     4141 gcagagcgaa ttgtgtttga aattgcagaa aaacgcaata atgccaatga agggccacaa
     4201 cgtattgacg atattttact taaaacactc gcccgaatgg attcgctatc taaaaatcgc
     4261 cataacggcg gagttactgg tgtttctacc gggtttgttg atctcaataa aaaaaccgca
     4321 gggttacagc cttcggattt aatcattgtg gcagcccgtc cttcaatggg gaaaactact
     4381 tttgcaatga acttatgtga aaatgcttca ttagtggata ttgaagataa agatgattta
     4441 gacgaacagg gtaatccgaa gaaaaaaccg gcaaagccag tgctaatttt cagcctggag
     4501 atgccggcag accaaattat gatgcgtatg cttgcctcac tttctcgggt cgatcaaacc
     4561 aagatccgaa ccggtcagat ttcagatgat gaagacactg ctaaaatctc cagcacaatg
     4621 gcaattttac aagaacgtaa taatatctat attgatgata gctccggttt aacaccgacc
     4681 gaattacgtt ctcgagcgag acggatttat cgtgaaaatg gcggattaag cttgattatg
     4741 gtggactatc tccaattaat gcgagcgccg ggatttgcag ataatcgtac tttagaaatc
     4801 gcagaaattt cccgttcttt aaaggcacta gcgaaagaat tagaagtgcc agtagtcgct
     4861 ctttctcagc taaaccggag tttggaacaa cgtgccgata aacgtccggt aaactcggat
     4921 ttgcgtgagt caggttctat tgagcaagat gcggacttaa ttatgtttat ttatcgtgat
     4981 gaggtctata acgacaattc cgatctgaaa ggcattgcgg aaattattat cggaaaacaa
     5041 cgtaacggcc cgattggtcg ggtgcgttta acgttccaag ggcagtattc ccgttttgat
     5101 aattacacag gagggcatta tgatgatgaa tactaaacca actcagccaa tcacactttg
     5161 tgcgatttat cgcagtaagg caaaagaagg aatgtattta tatgtgccta agcgtgacca
     5221 atttgagcaa gtaccggaaa cattacgcca gttatttggt aagcctgaat ttgtgatgat
     5281 gtttaattta accggtgaaa agccattggt gcgtgctaaa aacgaagaag tgttacaatt
     5341 acttatagag caaggctact atttgcaaat gccacctccg ccggagaatt tgcataaagc
     5401 ctttatcgca caacagaaag gggaaaaata atgcgctgcc cattttgttc cactgaagaa
     5461 actaaagtga tcgattcacg gcttgcagca agcggttatc aaattcgccg ccgccgtgaa
     5521 tgtataagct gtaaagaacg ttttactacc tttgaatcgg ccgaattagt tgtgccttac
     5581 gtgatcaaaa ataatggtag ccgagagccg tttgatgccg aaaaattaag aacagggtta
     5641 agacgtgcat taaataaacg ccctgtcagt gctgatgatg tcgaaaaagc aattagccaa
     5701 atcattattc agctgcaagc gatgggcgaa cgagaagtgc cgagtcgcca tatcggtaca
     5761 ttggcaatga acgcacttaa acaattagac aaagtagcat atatccgctt tgcctctatt
     5821 tatttaagct ttagtgatat tgaagagttc attaaagaaa ttgaaaaatt aaaagagtag
     5881 ttgcaaaaaa acataaaaaa atgaccgctt gtgagtttaa aatccacaag cggtcatttt
     5941 tctttaaaaa tactcaatta gcttttactt gttccaacac aaaccctaca aacgcccgca
     6001 caatgctaga cggataacga tgggcggcat aaatcatcgt caaatcaaat ttggggtttt
     6061 ctatctcgcc caagacttcc accaagccat tttctgccac gagtctatcc accatgctgt
     6121 cggcaagcag cgccactgct ttgccctgtt ttgccatata ttcaatggca tagctgtcat
     6181 tgctggcaaa aagataggga atttccaccg cttgtctgcc agtgccaagt gtaatgacgc
     6241 ttgcttcatt ttttgcccaa gcggcaatgg ggaaattttt caaatctttg atggtattgg
     6301 gcgtacccaa tcgtgccaat aaatcagggt gtgccaccca tttactgccg atttgcccca
     6361 cttttttggc gataaactgt tcgccgtgta actgcccaat ccgaaatgct acatcaatgc
     6421 cgtctgccga caaatcaagc agacggtcgg tcagcgtgca atgcacctgt acctgtgggt
     6481 aggctgcctg aaaatcagct atccacgcca aaacaggctc gcaggcaggg cttgccgaca
     6541 ggcgtaattt gccctttaaa ttgccttgct cttcaaacaa cacttctttg gcagtggcaa
     6601 gctgttcaat ggacaaatgc acctgttcat acaggcgttg ccctgccatt gtgggtttaa
     6661 cgcctgattt ttagtggtca aagagttgaa tgttaagctc tttttccaaa tcggcaattt
     6721 gacggctaat ggttgcaatc ggtacattga gtcgctcgga ggctttggag agactgccgt
     6781 gctggataac ggcgacaaag aggcggaggg tgttgaggtt cattgttttt ctccccaatg
     6841 ttcaatttct tgaataaaat
//
LOCUS       DANJWC010000125         6751 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.125, whole genome shotgun sequence.
ACCESSION   DANJWC010000125 DANJWC010000000
VERSION     DANJWC010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6751
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.125"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..272
                     /locus_tag="PWX02_001863"
     CDS             <1..272
                     /locus_tag="PWX02_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729849.1"
                     /translation="LFNNVFLTFIIPMMVSSAVIVYKGYTQKNEDKVFWIYMGFFLIW
                     ILGFIYFEPAVYDKSLKISGAGFGAMLSIIICSKLLPKETNKEQL"
     gene            complement(493..1581)
                     /gene="purK"
                     /locus_tag="PWX02_001864"
     CDS             complement(493..1581)
                     /gene="purK"
                     /locus_tag="PWX02_001864"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDL1729850.1"
                     /translation="MQKSAIYPPVYVLGNGQLGRMLRYAGAPLDIEVKPLAFDAPVFD
                     IEPNSIITAEIERWADTPLTQLLGNHPNFVNLNVFGKLADRFTQKSLLDELHLATSKW
                     QLLENAEHWQDIFQHIGEKVVVKRRTGGYDGRGQWIVTQDSIHQITPELFGEVIAEKF
                     IPFEGEISIVGARFRDGSTRFYPISHNLQQNGILRYSVSDVNLPNQALYQQQAEQMLA
                     TVMQKLEYVGVMAMECFVVGDKLLINELAPRVHNSGHWTQLGCAISQFELHLRALLDL
                     PTPALDQIAPSVMVNLIGIEHNKQWLSLPFSQLHWYGKEVRAGRKVGHINLTHADKNG
                     LIELLEQLRPSLTEDFHSGLDWAINKLK"
     gene            complement(1600..2565)
                     /locus_tag="PWX02_001865"
     CDS             complement(1600..2565)
                     /locus_tag="PWX02_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDL1729851.1"
                     /translation="MSKKLLILHTGGTISMKEGEDGKVSPSNENPLLAALERLNHPAK
                     LVQESLFNIPSPHINIEHWQQLKMRIEKAVNEENVDGIVITHGTDTLEETSYFLDLAL
                     SVNIPVVITGAMRSSNELGADGLINLQSAILVALNEESRDKGVLVVMNDEIHNAKFVT
                     KTHTTNVATFQTPTFGPCGLVTKESVIFFQKLTTYERYPIQQVTKANVQLLKAYAGMD
                     SFLLEQLAKSKCDGVVIEALGAGNLPPSCLAGIEALVNANIPIVIVSRAFNGITQDVY
                     DYVGGGKQLKQKGIIFTKGLSGQKARIKLMVLLSQTLDKPLSDYF"
     gene            complement(2580..3077)
                     /gene="purE"
                     /locus_tag="PWX02_001866"
     CDS             complement(2580..3077)
                     /gene="purE"
                     /locus_tag="PWX02_001866"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDL1729852.1"
                     /translation="MPNKAEIAIVMGSKSDWATMSEATQILDQFGLSYHVEVVSAHRT
                     PDKLFSFAETAETNGYKVIIAGAGGAAHLPGMIAAKTIVPVLGVPVKSSMLSGVDSLY
                     SIVQMPKGIPVGTLAIGPAGAANAGLLAAQILAAFNPDIAEKLRIFRQTQTQMVLDNP
                     DPRVE"
     gene            3251..3895
                     /gene="sspA"
                     /locus_tag="PWX02_001867"
     CDS             3251..3895
                     /gene="sspA"
                     /locus_tag="PWX02_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HDL1729853.1"
                     /translation="MTSSINKRSVMTLFSDKNDIYSHQVRIVLAEKGVPYEIENVMLG
                     SISEDLLELNPYGNIPTLVDRELVLFNPRIIMEYLDERFPHPPLMPVYPVSRGQCRLN
                     MYRIEQDWYSQIDIVNKNPESAEGKKALAQLKEDVLALAPIFAAKPYFMSDDFSLVDC
                     YIAPLLWRMQILGVKFSGATAKPINAYMTRVFQRDSFIQSVGGEAPKHLMDDKD"
     gene            3895..4302
                     /locus_tag="PWX02_001868"
     CDS             3895..4302
                     /locus_tag="PWX02_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HDL1729854.1"
                     /translation="MKPLRPYLYNAYYNWIIDSDNTPYLLVNAEFPDVDVPVEFVKEG
                     KIILNISPRSIGQYIVADEYISFSARFQGMLRDIYIPFGAMVAIYAQETGDGIMFQEE
                     AYYTEEAYQARVRAVEQPKKIKAKKTSTLKLVK"
     gene            4499..5458
                     /locus_tag="PWX02_001869"
     CDS             4499..5458
                     /locus_tag="PWX02_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1729855.1"
                     /translation="MKFHKTLLVTLLSSLSVAALAQDKIQNVAITAIVEHPALDSIRK
                     GVIEELAREGFVDGKNIKIDYQSAQGSTATAAQIARKFVGDKADIIIPITTPSAQPVV
                     AATRSIPIVFSGVTDPVAAKLVKSWEPSGTNVTGISDHKAMAPQVKLIQTLVPDLKAV
                     GYVYSAGEVNSAIVLEELKQEAQKQGFTIVPVAVQRSADIGTAARSLNGKVQAIYISE
                     DNAVVSAYEALHKAALEAKIPVIAADRDTVQRGAVAAYAVNQYDIGVATGKVAARVLK
                     GEKAGTIPIQEVSKLELSINTKTAKELGITIPEVLIKEAKQTF"
     gene            5538..5798
                     /locus_tag="PWX02_001870"
     CDS             5538..5798
                     /locus_tag="PWX02_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005718542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL1729856.1"
                     /translation="MGWIAAIIVGAIIGWIASAVMKSEGGLCKNIIIGIVGSSLGRWV
                     FGDVLGIGSAYSAGDFNIVGIFFGVLGAALLIFILRKLSIIN"
     gene            complement(5848..6738)
                     /locus_tag="PWX02_001871"
     CDS             complement(5848..6738)
                     /locus_tag="PWX02_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL1729857.1"
                     /translation="MDKLNAISLFCKVIETQSFTQAAQQAQISLPMASKLVAQLEEHL
                     NVRLLQRTTRKITPTEAGLLYYQQCLPILNELKEADASVSNINSTLQGKITMSVPMDF
                     GSRFIAPYLGKFMEDYPNLALHIEFSDRRVDVIAEGFDLVLRIGSLADSSIVAKKIAD
                     SHLVLVASPDYLARFGEPKGLEALVERGCLLYEYHPQWQFSQQGQKMQIKPQGKIYSN
                     NGYALVQMAKAGLGIINIPKFLIKHELKNGELIPIMPFLTQEPLQISLLYPNRRYLSP
                     KVRILIDFFTQLMQEQKEYI"
BASE COUNT         1931 a         1251 c         1447 g         2122 t
ORIGIN      
        1 ccctgttcaa taatgtgttt ttgactttta ttattccgat gatggtaagt agtgcggtta
       61 ttgtttataa aggttatacc cagaaaaatg aagataaagt gttttggatt tatatggggt
      121 tctttttaat ttggattttg ggatttattt attttgagcc ggcagtttat gataaatcac
      181 tgaagatttc aggtgcagga tttggggcga tgctctctat tatcatttgc tcgaagttat
      241 tgcctaagga aacaaataaa gagcagctct aagattcttg tgaagaaatc tgttttctcg
      301 gaggaagaaa atgctttttg atagccatat ccaccctgag gggttttcag atcaagatga
      361 tgaccggaag tagccattat gttgaattat tttgcaaatt ttagcactaa gtcgaccgct
      421 tgtttggtac gagcgaggaa gctcgcacca tcgggtaaac agatttgcaa aaaagttaca
      481 aaatccgacc gcttacttga gcttattgat tgcccaatct aatcccgaat ggaaatcttc
      541 cgttaggctc ggtctgagtt gttccagtag ttcaattaag ccgtttttat cggcgtgagt
      601 gaggttgata tgcccaactt ttctaccagc tcggacttct ttgccatacc aatgtagttg
      661 gctaaatggg aggcttagcc actgcttgtt atgttcaatg cctattaaat taaccatcac
      721 gcttggggca atttggtcga gtgctggcgt aggtagatct aataatgccc gtaaatgtaa
      781 ctcaaattgg ctgatggcac agccgagttg tgtccagtgt ccgctgttat gcactcgagg
      841 ggcaagctcg ttgatgagta gcttgtcgcc cacaacaaag cattccattg ccattacgcc
      901 gacatactct aatttttgca ttaccgtagc gagcatctgt tcggcttgct gctggtagag
      961 ggcttggttc ggtaaattca catcggagac ggagtaacgt agaatgccgt tttgttgtaa
     1021 attgtgagaa atagggtaga aacgggttga gccgtcacgg aatctcgccc caacaatgga
     1081 aatttccccc tcaaacggga taaatttttc ggcaatcact tcgccaaata attcaggtgt
     1141 aatttgatga atcgagtctt gcgttacaat ccattgccca cgtccgtcat atcctccggt
     1201 tcggcgttta accactactt tttcgccaat atgctgaaaa atatcttgcc aatgttcggc
     1261 attttctagc agttgccatt ttgaagtcgc aagatggagt tcatcaagta gtgatttttg
     1321 cgtaaaacga tcggctaact tcccaaacac attcaggttc acaaaattcg ggtggttacc
     1381 gagtaattgc gttaaaggcg tatctgccca acgttcgatt tctgcggtaa taatgctgtt
     1441 tggctcaata tcaaacacag gggcatcgaa agccagcggt ttgacttcaa tatcaagtgg
     1501 tgctccggcg tagcgtagca ttctcccgag ctggccgttg cctaatacat acacaggtgg
     1561 gtagattgca cttttttgca tcgtggtgtt ctcaatgggt tagaaatagt ctgatagtgg
     1621 tttgtctaag gtttggctga gtaagaccat aagtttaatg cgagcttttt gcccacttaa
     1681 gcctttggta aaaataatgc ctttttgctt taactgtttt ccaccgccta cataatcata
     1741 gacatcttgg gtaataccat tgaatgcccg agaaacaatt acaattggaa tattggcatt
     1801 cactaaagcc tcaataccag ccagacaact agggggtaaa ttacctgcac ccaatgcctc
     1861 aatcacaaca ccatcgcatt tgcttttcgc taattgttcc agcagaaaac tatccattcc
     1921 ggcgtatgct ttcagtagtt gcacgttagc tttagttact tgttgaattg gataacgttc
     1981 gtaagtggtc aatttttgga aaaaaatgac agattctttc gttactaagc cacaaggccc
     2041 aaaggttggg gtttggaaag tcgccacatt agtggtgtgg gttttggtca caaatttagc
     2101 attgtggatt tcatcattca tcaccactaa cacgccttta tctcggcttt cttcatttaa
     2161 cgccactaaa atcgcacttt gtagattgat taagccatca gcacccagtt cgttactgga
     2221 acgcattgct ccggtaatta ctaccggtat atttacgctg agggctaaat ccagaaaata
     2281 ggaggtttct tccaaggtat ctgtgccgtg ggtgattact atgccatcga cattctcctc
     2341 attcactgcc ttttcaatcc gcatttttaa ctgttgccaa tgctcgatat tgatatgcgg
     2401 agagggaata ttaaataacg attcttgcac caattttgct gggtggttta aacgttctaa
     2461 ggcagccaag agaggatttt cattggaagg ggagacctta ccgtcttccc cttctttcat
     2521 tgaaatagta ccaccagtgt ggaggattaa taatttttta gacatctttt atacaatagt
     2581 tattcaacac gaggatcagg attatctaat accatctgag tttgggtttg acggaaaata
     2641 cgcagtttct ctgcaatatc agggttaaag gctgctaaaa tttgagcggc taataagcct
     2701 gcatttgctg cacctgccgg cccgattgcg agcgtaccga ccggaatacc tttcggcatt
     2761 tgcacaatcg aatagaggct atctacacca cttaacattg agctttttac cggcacacct
     2821 aacaccggca caatggtttt agcagcgatc attccgggca agtgagctgc accgccggct
     2881 ccggcaataa tcactttata gccgttagtc tctgcagttt cagcaaaaga aaagagtttg
     2941 tccggtgtgc ggtgtgctga gacgacttca acgtgatagc tcaaaccgaa ctggtctaat
     3001 atttgggttg cttcagacat tgtagcccaa tcactcttag atcccattac gatcgcaatt
     3061 tcagctttat ttggcatttt aaatccttat gatactaatg taaaacagtg taatcatagc
     3121 atacaaagca aacgtttgcg tagctattta agaagtgaaa agcaattttt agggctgtta
     3181 tctgctggtt aaaatatgat ataatcgaca accattttat tttttataaa tttaatcatc
     3241 ggagatataa atgacgagta gtataaacaa acgttcggta atgactcttt tttcggataa
     3301 aaacgatatt tacagccacc aagtgcgtat tgtgttagca gaaaaagggg tgccttatga
     3361 aatagagaac gttatgctag gcagtatttc tgaagattta ttagaattga atccttacgg
     3421 taatattcct acattggttg atcgtgaatt agtgctgttt aaccctcgta ttattatgga
     3481 atatttagat gagcgtttcc ctcacccacc attaatgccg gtttaccctg tttctcgtgg
     3541 tcagtgccgt ttaaatatgt atcgtattga acaagattgg tattcgcaaa ttgatattgt
     3601 gaataaaaat cctgaatcag cggaaggcaa gaaagcgtta gcacagctta aagaagatgt
     3661 attggcatta gcgcctattt ttgccgctaa accttatttt atgagcgatg atttcagctt
     3721 ggttgattgc tatattgcac cgttattatg gcgtatgcaa atcttaggcg ttaaattcag
     3781 cggtgcaacg gcaaaaccaa ttaatgcgta tatgacccgt gtattccaac gtgatagttt
     3841 tatccagtct gttggcggcg aagcgccaaa acatttaatg gacgataaag actaatgaaa
     3901 ccattaagac cttatcttta taatgcttac tataactgga ttattgatag cgataacacg
     3961 ccttacttat tagtgaatgc agaatttcca gacgtagatg tgccggtaga gtttgtcaaa
     4021 gaagggaaga ttattttaaa tatttcgcct cgttccatcg ggcagtatat tgtggctgat
     4081 gaatacatta gctttagcgc tcgttttcag ggaatgttaa gagatattta tattcctttt
     4141 ggggcaatgg tggcgattta tgcgcaagaa accggtgatg gtattatgtt ccaagaagaa
     4201 gcctactaca ctgaagaggc ttatcaagct cgagttagag cagtagagca gcctaaaaaa
     4261 atcaaggcga agaaaacatc taccttaaag ttggtgaaat aagagatcta tttcatcaaa
     4321 acacattaca agcggttaaa tttttctgat tatttgcaaa tatcagtcaa aatttaaccg
     4381 cttgtttgtt ttgtgacgga aataatcaaa ttccacttgc actccgacta aaaattgctt
     4441 atctttgttt tgtgtttgta ctgttgtgat agtacagtaa tgatcttaag gagagaaaat
     4501 gaaattccat aaaaccttat tagtgacttt gttaagttca ctttctgtag cggcattagc
     4561 tcaagataaa attcaaaatg tagcaattac agcgattgtg gaacaccctg cattagattc
     4621 aattcgtaaa ggtgtgattg aagaattagc ccgagaaggg tttgttgatg gtaaaaacat
     4681 taaaatcgac tatcagtctg cacagggtag tactgcaaca gcagcacaaa ttgcgcgtaa
     4741 atttgttggg gataaagcag atattattat cccgatcacc acaccttcgg cacaacctgt
     4801 tgttgctgca acacgttcta tcccaattgt gttttcaggc gtgacagacc cggttgctgc
     4861 aaaattagta aaatcgtggg agccaagtgg cactaacgtg acggggattt cagaccacaa
     4921 agcgatggct ccacaagtga aattaattca aacattagta ccggatttga aagctgttgg
     4981 ttatgtgtat agtgcaggtg aagttaattc tgcgattgtg ttagaagagt taaaacaaga
     5041 ggctcaaaaa cagggcttta ctatagtgcc ggttgctgtt caacgcagtg ccgatattgg
     5101 cactgctgct cgcagcctaa acggaaaagt gcaggctatt tatatttctg aagataatgc
     5161 agttgtttcg gcttatgaag ccttacataa agcggcttta gaagcaaaaa ttccggtgat
     5221 tgctgctgac cgtgatacag tacaacgtgg tgcagtggca gcttatgcgg ttaatcaata
     5281 tgatattggt gtggcaaccg gtaaagttgc tgctcgtgta ttaaaagggg aaaaagcagg
     5341 tacaattcca atccaagaag tcagcaaatt agagctttca attaatacta aaacggcaaa
     5401 agaattgggc attactatac cagaggtatt aatcaaagaa gctaaacaaa ctttttagtg
     5461 taaaaaataa aaatttgttt tatttgtcat aatcttgtag agtaaaaatt catttcaact
     5521 cagctaagga gaatattatg ggatggattg ctgcaattat tgttggcgct ataattggtt
     5581 ggatagcttc ggctgtaatg aaaagtgaag gcggactttg caaaaatatt attattggta
     5641 ttgtgggttc gtcacttgga cgctgggtat ttggtgatgt attaggtatt ggttccgctt
     5701 atagtgccgg agactttaat atagtcggta tcttttttgg tgtattaggg gcagctcttt
     5761 tgattttcat tttgagaaaa ttaagcataa ttaactaatt tcgtatttca gaaaggaagc
     5821 agttgtttta ttactgcttc ttttttgtta aatatactct ttttgctctt gcatcagttg
     5881 tgtgaaaaaa tcaattagga ttcgcacttt aggagagaga taacgacggt ttggatagag
     5941 taagctgatt tgtagaggtt cctgtgtcaa aaaaggcata ataggaatca actctccgtt
     6001 ttttagctcg tgttttatta aaaactttgg aatgttaatg atgcccaaac cagcttttgc
     6061 catttgtact aacgcataac cattgttact gtaaatttta ccttgtggtt taatttgcat
     6121 tttttgtcct tgttgagaaa attgccattg aggatggtat tcatagagta ggcagcctcg
     6181 ttccactaat gcttctaagc ctttaggttc gccaaatcga gctaaataat ctggagaagc
     6241 aactaaaacc aaatgggaat ctgcaatttt tttcgctaca atggaactat ctgcaaggct
     6301 accaatacgc agcaccaaat caaagccttc tgcaatcaca tctacccgac ggtcactaaa
     6361 ttcaatatga agggctaaat tagggtaatc ttccataaat ttcccaaggt aaggggcaat
     6421 gaagcgagag ccaaaatcca ttggtacaga cattgttatt ttcccttgta gcgttgagtt
     6481 aatattactt acgctggcat cggcctcttt tagttcattt aaaataggca agcattgctg
     6541 atagtagagc aaccctgcct cagttggcgt aatttttcga gtggttcgtt gtagtaaacg
     6601 cacattgaga tgttcttcta actgagccac caatttactt gccatgggta acgaaatttg
     6661 agcttgttgt gctgcttgag tgaaactttg ggtttcaatt actttacaaa ataaactgat
     6721 tgcgtttagt ttatccattt tctctcttct t
//
LOCUS       DANJWC010000126         6707 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.126, whole genome shotgun sequence.
ACCESSION   DANJWC010000126 DANJWC010000000
VERSION     DANJWC010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6707
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.126"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..615)
                     /locus_tag="PWX02_001872"
     CDS             complement(<1..615)
                     /locus_tag="PWX02_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1729858.1"
                     /translation="MAEILFRSCLCAMQSVFFIYLQFYKGFETSQIATAFSLLVFTSQ
                     AFSLFAGSWGDSFGRYPIMMLGCLLDALAYILLLTTKNYPLLLLATFFFGLGSTLFST
                     NAKAFLLANIQDTGDSYQLKTKAQGKFLKVSSLSAMVAPLLTIPFIFYRKAEWLIWIS
                     CCIEIIMLFVMFKTAPKTHECANGQCERFKFGALRGILNEQFIFV"
     gene            698..1159
                     /locus_tag="PWX02_001873"
     CDS             698..1159
                     /locus_tag="PWX02_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDL1729859.1"
                     /translation="MRKIKLLHVILSLILGLSAAAIGSYPAIKEIGEERAKKHQDILD
                     LNRELYSAMVNRDFSALQTLLSDDFELYSSTGEVIDKQEWIKLIQKRVLEFDKVDLLS
                     TDFQGNQSSGLLRVNGEFFGIERDNVVVEMNIRTIVRNTERQIKCIVIKEV"
     gene            1172..2065
                     /gene="murQ"
                     /locus_tag="PWX02_001874"
     CDS             1172..2065
                     /gene="murQ"
                     /locus_tag="PWX02_001874"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL1729860.1"
                     /translation="MNALGQMITEQRNPNSMNIDRLSALDIVQVINQEDKQVAIAVEQ
                     CLPQIAQAVEKIVQAFEKGGRLVYVGAGTSGRLGVLDASECPPTYGVKPEMVVGLIAG
                     GERALRFPIEGAEDNREQGKVDLQAVNFSEKDVLVGIAASGRTPYVLGAMEYAKQLGA
                     TTVSIASNPNSAMAQIADIAIETVVGPEVLTGSSRMKSGTAQKLVLNMLTTASMILIG
                     KCYQNLMVDVQATNEKLKARAIRIVMQATECEREVAEDTLKLAENNAKLAIMMILGNL
                     DKQKAEDLLSQNQGRLQKALK"
     gene            complement(2151..3095)
                     /gene="prmB"
                     /locus_tag="PWX02_001875"
     CDS             complement(2151..3095)
                     /gene="prmB"
                     /locus_tag="PWX02_001875"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL1729861.1"
                     /translation="MFKYNTALLDEISALPIVNDLVTIQDMMRWGYSYFNASDLYYGH
                     GYDNAWDEAQQLVLAGLYLPFDVPETLYSSRLTRVEKERIINLICERLGSRKPVAYLT
                     NSAWFCGGEYYVDERVIVPRSPIGELIKKGFKGIIKNEPHRILDMCTGSGCIAIACAE
                     RFPEAEVDAVDLSLDALDVAQINIERHQVSHRVFPICSDLFNNLPQDKYDLIVTNPPY
                     VDEEDLGDMPEEFHHEPALALGSGKDGLDLTKRILAQAADYLNDDGVLVCEVGNSMVH
                     LIEQFPSVPFNWLEFEHGGLGVFSLTKEELVKHRHLFA"
     gene            3188..3733
                     /gene="smrB"
                     /locus_tag="PWX02_001876"
     CDS             3188..3733
                     /gene="smrB"
                     /locus_tag="PWX02_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HDL1729862.1"
                     /translation="MNDTLLADEFALFREAAKGTKKLTQNTFVPKAEPRKKLQELREL
                     KEKADTEFYFSDEYEPLLKEENEKVKYLREDVDPYILKQLRRGDFQPELFLDLHGLTR
                     EKAKKELAALILACEREKVYCASVMTGFGTRTLKDQIPRWLVQHPKVMALHQAPKEWG
                     GDASILILVEQPENPEKMFGR"
     gene            3797..4282
                     /locus_tag="PWX02_001877"
     CDS             3797..4282
                     /locus_tag="PWX02_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDL1729863.1"
                     /translation="MSEQTFHQITALLDSHRARYRVVEHPSAGKSEEVAKIRGTEIGQ
                     GAKALVCKVKGNGVKQAVLAILPADYQADLTKIAEHLGGLRASLASPAEVAELTDCVF
                     GAIPPFSFHPDLLLVAEPSLFERYAELAFNAGTLERSIILNTEDYQKIVNPVLVQFAK
                     S"
     gene            complement(4284..5033)
                     /locus_tag="PWX02_001878"
     CDS             complement(4284..5033)
                     /locus_tag="PWX02_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL1729864.1"
                     /translation="MEKHFEINEQGLSIRCKLFYEKELQQIENVVIVLHGFGSSKDLK
                     SNTKFGERLSSKYKGYAALAFDFPCHGDDARKKLSVEESITYLQLVIDYAKTSLNAKN
                     LYAYGTSFGGYLTLKYLAEKGNPFRKIALRAPAVQIYHSLMNRLSEEERQKLAKGREI
                     LWGFDRQMKISQAFFDELTDITQNDYLDYAEQILILHGTEDEMVALSTSQQFADNNVI
                     ELLPIEGADHAFSNPKLMDIAIGKIVEFFAP"
     gene            5205..6068
                     /locus_tag="PWX02_001879"
     CDS             5205..6068
                     /locus_tag="PWX02_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HDL1729865.1"
                     /translation="MLQLILAILCSVTVGVLIKFARQKGIRIDQSIAVNYIVTTTMTF
                     LLLKPDYQGQSITDIIANNPTSYIFVILSITLPTIFLVQAKALEFAGIIRTDAAQRLS
                     LFLPILAAFTIFGEQVTQNKLIALLLAFVALGCLLWKGNQGMEKGGKIAIISLALVWV
                     GYGVNDILFKQIAKSGSAFPVTLSIAFIGAGCFMFIYLLLKRTQWHVPSVLTGLLLGV
                     LNFGNILFYIQAHQAMKDDPTLVFTGMNLGVICLGTFVGAFFFKEKIHKVNYLGVAIA
                     IVAIICLFYWR"
     gene            6192..>6707
                     /locus_tag="PWX02_001880"
     CDS             6192..>6707
                     /locus_tag="PWX02_001880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729866.1"
                     /translation="MKLKALSTLIASSMLYSQSFAVPPEKTAEEPQPFFNKTESGYDL
                     VFNDQNYRLIETELNGEKIQFRAFERIVYVNNPIEPDYQTINLYVPEAYYNNGEINGY
                     QADTAPIFLPNSVGAICRQKRQVMIKKDVTVHQVRFWWRYLKAMWWQVWEQEDARYKK
                     RENTPAKHLLPL"
BASE COUNT         2068 a         1268 c         1490 g         1881 t
ORIGIN      
        1 tacaaaaata aattgttcat ttagtattcc tcttaatgca ccaaatttaa agcgttcgca
       61 ttgcccattt gcacattcat gtgttttggg tgcggtttta aacataacga atagcataat
      121 aatttcaatg cagcagctaa tccaaataag ccattctgct ttacggtaga aaataaatgg
      181 aatagtaagt aatggtgcta ccatcgctga caggcttgat accttcagaa atttaccttg
      241 agccttagtt ttgagttggt aactatctcc ggtatcttga atattggcta ataagaatgc
      301 tttcgcattg gtactaaata aggtgctacc gagcccgaag aaaaaggtag caagtaatag
      361 cagagggtaa ttttttgttg tcagtaataa aatgtaagct agggcatcga gcaaacagcc
      421 aagcatcatg atagggtaac gtccaaaact atcaccccaa gaacctgcaa aaagggaaaa
      481 tgcttggctg gtaaagacca acaaagaaaa tgcagttgca atttggctgg tttcaaagcc
      541 tttgtagaat tgtaagtaga taaaaaatac gctttgcatt gcacataagc aagaccggaa
      601 aagaatttcc gccataaaat gatattttgg atattcatag ataatattta atgagaaaaa
      661 tgatttctaa tataactaaa tttaataagg aagcacaatg agaaaaatca aattattgca
      721 tgttatttta tctttaatat tagggctttc tgcggcagcg attggaagtt atccggcaat
      781 aaaagagatt ggcgaggagc gggcaaaaaa acatcaagat atattagatt taaatagaga
      841 actttatagt gcaatggtga accgtgattt ttcggctttg caaacattac taagcgatga
      901 ttttgagctt tattcatcaa caggtgaagt gattgataag caagaatgga tcaaattaat
      961 ccaaaagaga gtattggaat ttgataaggt tgatttgcta tctaccgatt ttcaggggaa
     1021 tcaatcatct ggattattaa gagtaaatgg tgagtttttt gggatagagc gggataatgt
     1081 ggtggtagaa atgaatattc gaaccattgt gagaaataca gaacgccaaa ttaaatgtat
     1141 tgtgattaaa gaggtttaag tgtcgcaact attgaatgcc ttagggcaga tgattaccga
     1201 acaacgtaat ccgaattcga tgaatattga ccgcctttca gcgttagata tagtccaagt
     1261 catcaaccaa gaagacaaac aagtagcgat tgcggtggag cagtgcctgc ctcaaattgc
     1321 acaagcggtg gaaaaaattg tgcaagcctt tgagaaaggt ggacgattgg tttatgtcgg
     1381 tgccggcacg agcggaagat taggggtgct tgatgcttcg gaatgtccgc ccacttatgg
     1441 cgtaaaacct gaaatggtgg ttggattaat tgccggtggt gagcgggcat tacgtttccc
     1501 gattgaaggg gcggaagata atcgtgagca aggcaaagta gatttacaag cggtcaattt
     1561 tagcgaaaaa gatgtattgg tcgggattgc agccagtggg cgaacgcctt atgtgttagg
     1621 ggcgatggaa tatgctaagc aacttggtgc gaccacggtg tcgattgcca gtaacccaaa
     1681 ttcggcaatg gcacaaattg cggatattgc gattgaaacc gttgtcgggc cggaagtgct
     1741 aaccggttcc agccgtatga aatccggcac agctcaaaaa ttggtattga atatgctcac
     1801 cactgcgtcg atgattttaa tcggtaaatg ctaccaaaat ttaatggtag atgtgcaagc
     1861 aactaatgaa aaactcaaag ctagagcaat tcggattgtg atgcaagcga cagaatgtga
     1921 acgagaagtg gctgaagata cactaaaatt agctgaaaat aacgctaaat tggcaattat
     1981 gatgatttta ggtaacttag ataagcagaa agccgaagat ttattaagcc aaaatcaagg
     2041 gcgattacaa aaagcgttaa aatagaagta tgtctgctct ctccccttgt gggagagaga
     2101 cagatcaatc attcattcag aatgattgat cagagagagg ggaggcagat ttaagcaaat
     2161 aaatggcgat gtttcactag ttcttctttg gttaaactga acacccctaa gccaccgtgt
     2221 tcaaattcca gccagttaaa cggcacactg gggaattgtt cgattaaatg caccatactg
     2281 ttgccgactt cgcagactag cacgccatca tcatttaagt aatcagcggc ttgagcaaga
     2341 atccgcttgg ttaaatctaa cccatctttg cccgagccaa gagctaatgc cggctcgtga
     2401 tggaactctt cgggcatatc gcctaagtct tcttcatcaa cataaggcgg attagtcaca
     2461 attaagtcgt atttatcttg cggtaaattg ttaaacagat cggaacagat cgggaaaact
     2521 cggtgagaca cctgatgacg ctcaatatta atttgagcaa cgtctaaggc atcaagggaa
     2581 agatcaaccg catcgacttc tgcttccgga aaacgctcgg cacaggcaat cgcaatacaa
     2641 ccgctgccgg tacacatatc taaaattcga tgtggctcgt ttttgataat gcctttaaac
     2701 ccttttttga tcagctcacc aatcggtgaa cgtggcacga tcacacgctc atcgacataa
     2761 tattcaccgc cacaaaacca agccgagttg gtaaggtatg ctacaggttt gcgagagcct
     2821 aaacgctcac agattaaatt gatgatacgt tctttctcaa ctcgagttaa gcgagaggaa
     2881 taaagtgttt ccggtacatc aaacggcaaa taaagccctg ccaacactaa ttgttgtgcc
     2941 tcgtcccacg cattatcgta gccgtgcccg taataaagat cagaggcatt aaaatagcta
     3001 tacccccaac gcatcatatc ttgaatagtg actaaatcat tcacaatggg cagggcggaa
     3061 atttcatcaa gaagtgcggt attatattta aacattggcg tgcctttttt cgtattgtta
     3121 aaatggctgt gttataccac attttgcttt gtgttagaat gtaaaaatga ataagaagga
     3181 aaagataatg aacgatacgt tattagccga tgaatttgca ttattcagag aggcagcaaa
     3241 aggcacgaaa aagctgacac aaaatacctt tgtgccgaaa gcggaacctc gcaagaaatt
     3301 gcaagaattg cgagaattaa aagaaaaagc tgatacagaa ttttatttct cggatgaata
     3361 tgagcctttg ctgaaagaag aaaatgaaaa agtgaaatat ctgcgagaag atgttgatcc
     3421 ttatattctc aaacagttac ggcgaggcga tttccaaccc gagctgtttt tagatctgca
     3481 tggtttaact cgagaaaaag cgaagaaaga gttggctgcg ttgattttag cctgtgagcg
     3541 ggaaaaggta tattgtgcca gtgtgatgac cggctttggc actcgcacgc tcaaagatca
     3601 aattccacgc tggttggttc agcacccaaa agtaatggcg ttgcaccagg ccccgaaaga
     3661 gtggggcgga gatgcctcta ttctaatttt ggttgagcag cctgaaaatc ctgaaaaaat
     3721 gtttgggcgt taaaattcgg ctgaattgcc aattttttat taaaaacgac cgcttgtgta
     3781 tcattaagga gaaatgatgt cagaacaaac tttccaccaa attacagcat tattagattc
     3841 gcaccgagct cgctatcgag tggtagaaca tccttctgcc gggaaatcgg aagaagtggc
     3901 aaaaattcga ggaacagaaa tcgggcaagg ggcgaaagcc ttagtttgca aggtaaaagg
     3961 caatggggta aagcaagctg tgcttgctat attacctgct gattaccaag cggatttaac
     4021 aaaaattgca gagcatcttg gtgggttaag agcctcactt gccagcccgg ctgaagtggc
     4081 agaattgacc gattgtgtat ttggggcaat tccgccgttt agctttcacc ccgatttatt
     4141 attggtggct gagccaagtc tgtttgagcg ttatgctgaa ttagccttta atgccggtac
     4201 gttggaacgt tcgattattc tgaataccga ggattaccaa aagatagtta atcctgtgtt
     4261 ggttcaattc gccaaaagct aggttatggg gcgaaaaact ccacgatttt gccaatagcg
     4321 atatccatta atttagggtt agaaaaagcg tgatctgccc cttcaatcgg gagcagttca
     4381 atcacgttat tatcggcaaa ctgctgtgaa gtgcttaacg ccaccatttc atcttccgtg
     4441 ccgtgcagaa ttaaaatttg ctcggcataa tccaaataat cgttttgggt aatatcggtt
     4501 agctcatcaa aaaaggcttg gctgattttc atctggcgat caaaccccca caaaatttct
     4561 cgccctttgg caagtttttg ccgttcctct tctgacaagc ggttcattaa cgaatggtag
     4621 atttgtactg ccggtgcccg caatgcaatt ttccgaaacg gattgccttt ttccgccaga
     4681 tatttcaagg ttaaatagcc gccaaaacta gtgccgtagg catacagatt tttagcattc
     4741 aggctggttt tggcataatc aatcaccagt tgcagataag taatggattc ctcaaccgag
     4801 agttttttac gggcatcatc accgtggcaa gggaaatcaa aggcaagggc agcgtagcct
     4861 ttatattttg agctaaggcg ttccccaaat ttggtattcg atttcagatc tttagacgaa
     4921 ccaaagccgt gcaacacaat cactacattc tcgatctgct gcaattcttt ttcataaaac
     4981 agtttacagc gaatgctgag accctgttca ttaatctcaa agtgtttttc cattttatgt
     5041 taagtattga aattgaagat gacaaaattt tagcactatt tgagtgcaag cggtcggatc
     5101 tctcgcaaaa tttgcaaatt ttccggtaaa tttaaccgct tgtactagcc aaaatttaag
     5161 catatttata gaatacagcc ttcgttttta aaaggagaaa tgaaatgtta caacttattt
     5221 tagcgatttt atgtagcgtg actgtaggcg tgttgattaa attcgcccgc caaaaaggga
     5281 ttcgtatcga ccaaagtatt gcggtgaact atatcgtgac taccacgatg acgtttttat
     5341 tactaaaacc ggattaccaa gggcagtcga ttaccgatat tattgctaat aacccgactt
     5401 cctatatttt tgtgatttta agtatcacat tgcctactat tttcttggtg caagcaaaag
     5461 ccctagagtt tgccggtatt atccgaaccg atgcggcaca acgtttatcg ctgttcctgc
     5521 cgattttggc ggcattcacc atttttggtg agcaggttac gcaaaataaa ttaattgcac
     5581 ttctgttagc tttcgtggca ctcggttgct tattatggaa aggcaatcag ggaatggaaa
     5641 aaggcggaaa aattgcgatt atcagccttg ctctggtttg ggtaggctat ggcgtgaacg
     5701 atattttatt taaacagatt gccaaatcag gctctgcttt ccctgtcacc ctttcgattg
     5761 cctttatcgg tgcaggctgt tttatgttta tctatttgct acttaaacgc actcaatggc
     5821 acgttccaag tgtcttaacc ggcttattgc taggcgtgtt gaacttcggc aatattctgt
     5881 tctatattca agctcaccaa gccatgaaag atgacccaac tttagtcttc actggaatga
     5941 acttaggggt aatttgctta ggcactttcg ttggagcatt cttctttaaa gagaaaatcc
     6001 acaaagtgaa ctacttaggc gtggcgattg cgattgtggc gattatttgc ttgttttatt
     6061 ggcggtaaat atactttcca aaattaaggg cgaacattat gttcgccctt aatttttgag
     6121 taatatagtg taaaatattc aataaattcg accgcttgtt gtaccgattc atatttcata
     6181 aaggacaaaa aatgaagcta aaagccttat ctacactcat tgcctcttca atgctatata
     6241 gtcagagttt tgctgtaccg ccggaaaaaa cagctgaaga gccacagccc tttttcaata
     6301 aaaccgaatc tgggtatgat ttagtcttta atgatcaaaa ctatcgttta attgaaacgg
     6361 aattaaatgg cgagaaaatc caattccgtg cctttgagcg gattgtgtat gtgaacaatc
     6421 cgattgagcc agattaccaa acaattaatc tgtatgtgcc ggaagcttac tacaataatg
     6481 gcgaaatcaa tggctaccaa gcggataccg ccccgatctt cttgccaaat tcagtggggg
     6541 ctatatgccg gcaaaagcgg caagttatga taaaaaaaga cgtgacggtt caccaggtac
     6601 gattttggtg gcgttatcta aaggctatgt ggtggcaagt gtgggagcaa gaggacgcac
     6661 gctacaaaaa gagggaaaat acaccggcaa agcacctttt gccatta
//
LOCUS       DANJWC010000127         6671 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.127, whole genome shotgun sequence.
ACCESSION   DANJWC010000127 DANJWC010000000
VERSION     DANJWC010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6671
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.127"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..271
                     /gene="rbsC"
                     /locus_tag="PWX02_001881"
     CDS             <1..271
                     /gene="rbsC"
                     /locus_tag="PWX02_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005753457.1"
                     /note="functions to transport ribose at high affinity;
                     forms a complex with RbsA2C2B; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDL1729867.1"
                     /translation="IVTSRLSSAQPTAGVSYELDAIAAVVVGGTSLMGGKGRVMGTLV
                     GALIIGFLNNALNLLDISSYYQMIAKALVILAAVLADNYLGTKKV"
     gene            294..1172
                     /gene="rbsB"
                     /locus_tag="PWX02_001882"
     CDS             294..1172
                     /gene="rbsB"
                     /locus_tag="PWX02_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006995182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HDL1729868.1"
                     /translation="MKKLTTIASAVMLSLSVAATASAKETIALAVSTLDNPFFVSLKD
                     GAQKKADELGYKLVVLDSQNDPAKELSNVEDLTVRGAKVLLINLTDSEAGGNAVAIAN
                     RNKIPVITLDRGAAKGEVVSHIASDNVAGGKMAGDFIAQKLGSGAKVIQLEGIAGTSA
                     ARERGEGFKQAIEAHKFNVLASQPADFDRTKGLNVTENLLSSKGDVQAIFAQNDEMAL
                     GALRAVSSAKKEVVIVGFDGTDDGVKAVESGKLAATIAQQPELIGSLGVETADKVLKG
                     EKVEAKIPVDLKVISK"
     gene            1293..2222
                     /gene="rbsK"
                     /locus_tag="PWX02_001883"
     CDS             1293..2222
                     /gene="rbsK"
                     /locus_tag="PWX02_001883"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDL1729869.1"
                     /translation="MKKLTVLGSINADHVISVPYFAKPGETLTGHSCHIAYGGKGANQ
                     AVAAARLGAKVSFIGCIGSDDIGKAMKSAFEQDGIDTRSIKTIPNEMTGIAMIQVAES
                     GENSIVISAGANGHLDETVVAKCAAEITQADYLLMQLETPLPAIVQAAKLAQQNGTKV
                     ILNPAPARALPDELLNLLDMITPNETEAEILTGVKVVDEASAIQAAQVFHQKGIEKVL
                     ITLGSKGVFVSENGVGKIVAGFRVQAVDTTAAGDTFNGALVTAMLEDKPLDQAIRFAH
                     AAAAISVTRKGAQPSIPTRQETLDFLSQQAVSF"
     gene            2236..3105
                     /locus_tag="PWX02_001884"
     CDS             2236..3105
                     /locus_tag="PWX02_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL1729870.1"
                     /translation="MATMKDIARLAQVSTSTVSHVINNSRFVSEEIRERVQRVVKELN
                     YTPSALARSLKVNETKTIGMLVTATGNPFFAEVMAGVEQYCQQHQYNLIIATTNGDTE
                     LNADKIFENSISGGYLATKTLIEAGHSKIAIITGNLKKSLAKNRLEGYKKALAEANIP
                     LNPNWVFESHFDFAGGVNGMNALLAQSEKPTAVFACSDTIALGAYQAIWQQGLRIPQD
                     ISIIGYDDIELAKYLSPPLSTISQPKAELGRLAVETLLKRIRFKSTDFQTIMLEPELV
                     LRHSIQTINKNAE"
     gene            complement(3141..3770)
                     /locus_tag="PWX02_001885"
     CDS             complement(3141..3770)
                     /locus_tag="PWX02_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL1729871.1"
                     /translation="MTAPTQILLIDDHPLMRQGMRQLLELDHQFSVVGEASNGTDGIK
                     LALRLEPDVIILDLNMKGLSGLDTLRSLRAQGIDARIIVLTVSDEQSDVFALMDAGVD
                     GYLLKDTDTPELLDNIRKAAAGEVVLSETVRQHLLNRQPENDPLSCLTDRELDVLQWL
                     ATGMSNKQIAAQLFISEETVKVHIRNLLRKLNVHSRVAATVLYLEQQKG"
     gene            3968..6127
                     /gene="rlmKL"
                     /locus_tag="PWX02_001886"
     CDS             3968..6127
                     /gene="rlmKL"
                     /locus_tag="PWX02_001886"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HDL1729872.1"
                     /translation="MTNQTTYFATAARGFEEILKTELEQICAAECKVAQGGVHFTTTQ
                     KGAYQALLHSRLASRILLPLISTKIFSDSDLYASIVSFNWADLFDPRDTFYIDFSGTN
                     REIRNTQFGAMRVKDGIVDYFERKGFARPTVDKDNPDVRIHVYLDRENVVVSLDLSGD
                     ALHIRGYREETGKAPLRETLAAAIILRSGWKVGTPLVDPMCGSGTLLIEAAQMAANIA
                     PQLHRKHWGFNAWKGHQQAMWKAVLDEAFRNVELGAVGENCNSSLQKMFFGFDLDHRV
                     LTKAKQNAKNAGVDHLIQWQQGDVAALKNPIPEKIGTVVCNPPYGERLGTTPALIALY
                     SVFGQRLKQQFAGWNASIFSGEPELLNCLRLRSARQFKAKNGPLDCVQKNYSISEHNK
                     RSDLDENLQFEQNAQVAPDFANRLTKNIKKIEKWAKQQGIDAYRLYDADLPEYNLAVD
                     RYGDHIVVQEYQAPKNIDEQKARQRLLDAVSATLYVTGVETNKLVLKVRQKQKGTNQY
                     EKLANKGEYFYVNEYGAKLWVNLTDYLDTGLFLDHRLTRKMVGEMAKGKTFLNLFAYT
                     GSATVHAALNGAKSTTTVDMSNTYLNWAEQNLELNNLSLRHHRLFQADCLQWLAECRE
                     RFELIFVDPPTFSNSKRMENSWDVQRDHIELMKQLKRILTADGTIVFSNNKRGFKMDF
                     DGLAALGLTAENISHKTLPLDFERNPQIHNCWIVKNV"
     gene            6304..>6671
                     /locus_tag="PWX02_001887"
     CDS             6304..>6671
                     /locus_tag="PWX02_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HDL1729873.1"
                     /translation="MLDIVLYEPEIPQNSGNIIRLCANCGFRLHMIEPFGFGWDDKKL
                     RRSGLDYHEFVDIQKYKNFEDFLERAKPKRLFALTTRGEQNHSDVKYELGDFLMFGPE
                     SRGIPKPLLAEMPMSQKIRIP"
BASE COUNT         1964 a         1342 c         1567 g         1798 t
ORIGIN      
        1 aattgtgact tcacgtctct cttctgctca gccaacggca ggtgtgtcct atgagttaga
       61 cgcaattgca gcggtagttg ttggtggaac cagtctaatg ggcggtaaag ggcgtgtaat
      121 gggaacgctt gtcggggcat taattatcgg tttcttaaac aatgccctta atttattgga
      181 tatttcttca tattatcaga tgattgctaa agcgttggtt attttagcag ctgtattagc
      241 tgataattat ctaggcacta aaaaagttta atctatcttt aaaggagatt tctatgaaaa
      301 aattaactac gattgcatca gcggttatgt taagtttatc ggttgcagcc actgcttcgg
      361 caaaagagac tatcgctctt gcagtatcga ctttagataa cccattcttt gtgagcctta
      421 aagacggtgc acagaaaaaa gcagatgaat taggctacaa attagtggtg cttgattccc
      481 aaaatgaccc ggctaaagag ctttccaatg tggaagactt aacggtgcgt ggggcaaaag
      541 tgttattgat taacctaacc gattcggaag cgggcggaaa tgcggtggct attgcaaatc
      601 gcaacaaaat tccggttatt acacttgacc gtggtgcagc aaaaggcgaa gtggttagcc
      661 atatcgcttc cgataacgtt gccggcggca aaatggcggg agatttcatt gctcagaaat
      721 taggctcagg ggcgaaagtg attcaattag aaggcattgc cggcacttcc gccgctcgtg
      781 aacgtggtga aggctttaaa caagcgattg aagcacataa attcaatgta ttagccagtc
      841 aaccggctga ttttgaccgc actaaaggct taaatgtgac tgaaaacttg ctctcaagca
      901 aaggggacgt acaagctatt tttgctcaaa atgatgaaat ggcactagga gctttgcgtg
      961 cagtgagttc agcgaagaaa gaggttgtaa ttgtgggctt tgacggcaca gatgatggcg
     1021 taaaagccgt tgaaagcggt aaacttgccg caacgattgc tcaacaacct gaactgattg
     1081 gctcattagg cgtagaaact gccgataaag tgctaaaagg cgaaaaagtg gaagcaaaaa
     1141 ttccggttga tttaaaggtt atttcaaaat aattatactt gaagatagcg ttcgtctctg
     1201 acgaacgctt tgaattaata aaatagcact cgtcagagac tagtgctatc agaatcctag
     1261 ttttgttgta ctaggtaatt tgagggctta atatgaaaaa actcaccgtt ctcggtagca
     1321 ttaacgctga tcacgtgatt tccgttccct attttgccaa accgggggaa acgctcaccg
     1381 gacatagctg ccacattgct tatggcggta aaggggcaaa tcaggcggtg gcggcagcac
     1441 gtttgggagc taaggtttct ttcatcggtt gtattggcag tgatgacatc ggcaaagcaa
     1501 tgaaatcagc ctttgagcaa gatggcattg acactcgctc gattaaaacg attccgaatg
     1561 aaatgaccgg cattgcaatg attcaagtgg cggaatcggg cgagaacagt attgtgattt
     1621 cagccggagc gaacggtcat ttggatgaaa cggttgtggc aaagtgtgca gccgaaatca
     1681 cacaggcgga ttatttgcta atgcagctag aaacaccgtt gccggcgatt gtgcaagcag
     1741 caaaattggc tcagcaaaac ggcacgaaag taatattaaa ccctgcgcct gctcgagcgt
     1801 tacccgatga actgttaaac ttactggata tgattacccc aaatgaaacg gaagcggaaa
     1861 tattaaccgg cgtgaaagtt gttgatgaag ccagtgcgat tcaagccgca caagtgtttc
     1921 accaaaaggg catcgaaaaa gtgttgatta cacttggctc aaaaggggta tttgtaagcg
     1981 aaaacggtgt aggcaaaatc gttgcaggct tccgtgttca agcggtggat accactgccg
     2041 caggggatac ttttaacgga gcattagtga ctgcaatgtt agaagataaa ccgctggatc
     2101 aagccattcg ttttgctcac gcagcggcag cgattagtgt gactcgcaaa ggggctcaac
     2161 cttctattcc gacccgtcag gaaacactgg attttctaag ccaacaagcg gtgagttttt
     2221 aggaaaattt tgcaaatggc aacaatgaag gatattgcac ggcttgcaca ggtttccacc
     2281 tcgaccgttt ctcacgttat caacaatagc cgatttgtca gcgaggaaat tcgggaaaga
     2341 gtgcagaggg tggtgaaaga actgaattac acgccttctg cattggctcg tagcctcaaa
     2401 gtgaatgaaa ctaaaaccat cggaatgctg gtaacggcaa ccggcaatcc gttttttgcg
     2461 gaggtgatgg cgggggtgga gcaatattgc cagcaacatc aatataacct gattattgcc
     2521 actaccaacg gtgacaccga gctcaatgca gataaaatct ttgaaaattc aatatccggc
     2581 ggctatctcg caacaaaaac cttaattgaa gcagggcatt ctaagattgc gattattact
     2641 ggaaacctaa aaaaatcatt ggcaaaaaat cgtttagaag gctacaaaaa agcacttgcc
     2701 gaggcgaata ttccactcaa tccaaactgg gtgttcgaaa gccattttga ttttgccggc
     2761 ggggttaatg gaatgaatgc tttgcttgcc caaagcgaaa agccgacagc ggtatttgcc
     2821 tgtagcgaca ccattgctct aggggcttac caagcaatct ggcaacaagg tttacgaata
     2881 ccgcaagata tttcgattat cggctatgac gatattgaac tggcaaaata cctttcccca
     2941 ccgctgagta ccattagcca gcccaaagcg gaactcgggc ggcttgcggt ggaaactttg
     3001 ctaaaacgaa ttcgttttaa atccactgat tttcaaacca taatgcttga acctgaatta
     3061 gttttaaggc attcaatcca aacaataaac aaaaacgctg aataatgtga agttattcag
     3121 cgttttttat ccgatttcaa ttagcctttt tgctgttcca aataaagcac ggtggcggca
     3181 actcgggaat gcacgtttaa tttacgcagt aaattgcgga tatgcacttt tacggtttct
     3241 tcggaaataa agagctgagc cgcaatttgt ttgttcgaca taccggttgc gagccattgt
     3301 aaaacatcca gttcacgatc tgttaagcaa cttaaaggat cattttctgg ctggcggttg
     3361 agtaaatgtt ggcgaacggt ttcgcttaat accacttcac cggcagccgc tttacggata
     3421 ttatctagta gttccggggt gtcggtatct tttaataaat agccgtccac accagcatcc
     3481 attaatgcaa atacatcgga ttgctcgtct gacactgtta aaacaatgat tctggcatct
     3541 ataccttgag cgcgcaatga gcgtaaagta tctagccctg aaagcccttt catatttaaa
     3601 tccagaataa tcacatccgg ctcaagtcgt aatgcaagtt tgataccgtc ggtgccgttg
     3661 cttgcttcac ccactacact aaattggtga tctaattcca atagttgacg cattccctga
     3721 cgcattaatg ggtggtcgtc aatgagtaaa atttgagttg gagctgtcat ttatttctcc
     3781 tttcctttct cttaatcggt taattttgca attattaatt caaaatagca agtttaggga
     3841 tttactaaat gccgcttttt tgatcttagc taatttttaa ctaaaaaaga aattgcaaaa
     3901 ctttgcaaga aaaagaccgc ttgttcggct ataattactg ccaattttta tttcaagtgt
     3961 gaaaagaatg acaaatcaaa caacctattt tgccacggca gcccgtggtt ttgaggaaat
     4021 actaaaaacc gaactggagc agatttgtgc ggcagaatgc aaagtcgctc aaggcggtgt
     4081 gcattttacc accacgcaaa aaggggctta tcaggcatta ttacacagcc gtttagcctc
     4141 tcggatctta ctgccgctca tcagcaccaa aatttttagt gatagcgatt tatatgcttc
     4201 catcgtgtca tttaactggg cggatttatt cgatccccgt gatacctttt acattgattt
     4261 cagcggcact aaccgagaaa tccgcaacac ccaatttggg gcaatgcgag tgaaagacgg
     4321 cattgtggat tactttgaac gcaaaggttt tgcccgccca acagtagata aagataatcc
     4381 ggatgttcgc attcatgttt atttggatcg tgaaaatgtt gtggtttcgc ttgatttaag
     4441 tggagatgcc ttacatattc ggggctatcg ggaagaaacc ggtaaagctc cgttgcgtga
     4501 aacattggcg gcagccatta ttttgcgttc cggctggaaa gtcggcacgc cattggttga
     4561 tccgatgtgt ggttcaggca cgttactgat tgaagcagca caaatggcag cgaatattgc
     4621 ccctcaactt caccgtaaac attggggctt taatgcgtgg aaagggcatc aacaagcgat
     4681 gtggaaagcc gtgttagatg aggcattccg aaatgttgaa ttgggtgcgg taggggagaa
     4741 ttgcaattcg tccctacaaa aaatgttctt cggttttgat ctcgatcatc gtgtgctgac
     4801 taaagcaaag caaaatgcga aaaatgcagg ggtagatcat ctcattcaat ggcaacaagg
     4861 tgatgtggct gcgttaaaaa atccgattcc ggaaaaaatc ggcacggtgg tgtgtaaccc
     4921 cccttatggt gaacgtttag gcacaacgcc ggcgttaatt gctctttatt cggtgttcgg
     4981 gcaacgccta aaacagcaat ttgccggttg gaatgcctca atttttagcg gcgagcctga
     5041 attgttaaat tgcctgcgtt tacgttcagc ccgtcaattc aaagcgaaaa acggtccgtt
     5101 ggattgtgtg cagaaaaatt actccatcag cgagcataac aagcggtcag atttagatga
     5161 aaatttacaa tttgaacaga atgctcaagt cgctccggat tttgcaaatc gcttaaccaa
     5221 aaatatcaaa aagatcgaaa aatgggcgaa acaacaaggc attgatgcct accgtttata
     5281 cgatgccgat ctgcctgaat ataatttagc ggtggacaga tatggcgacc acattgttgt
     5341 gcaggaatac caagccccga aaaatattga tgagcaaaaa gcccgccaac gcttgttaga
     5401 tgcagtctct gctacgcttt atgtgacagg tgttgaaact aataaattag tgttgaaagt
     5461 tcgccaaaaa caaaaaggca cgaatcagta cgaaaaactt gccaataaag gcgagtattt
     5521 ctatgtgaac gagtatgggg cgaaactgtg ggtgaattta accgattact tggatacggg
     5581 tttgttccta gaccatcgct taacccgtaa aatggtgggc gaaatggcaa aaggcaaaac
     5641 attcctgaat ctgtttgcct acacgggttc agctacggtt cacgctgctc ttaatggggc
     5701 aaaatcgacc actacagtgg atatgtcgaa cacctatttg aactgggcgg agcaaaattt
     5761 agagctgaat aatttatcac ttcgccatca tcgtttgttc caagccgatt gcttgcaatg
     5821 gctggcagaa tgccgtgagc gatttgagct gatttttgtc gatccgccga ctttctccaa
     5881 ctcaaaacga atggaaaaca gttgggacgt gcaacgagac catattgagt taatgaaaca
     5941 gcttaaacgt attttaaccg ctgatggtac cattgtgttc tcgaacaata agcgtggctt
     6001 taaaatggat tttgacggtt tggcagcatt aggcttaacg gcggaaaata tttcgcataa
     6061 aaccttaccg ttggattttg aacgcaatcc gcaaatccat aattgttgga ttgtgaagaa
     6121 tgtatagata ataccctcga cccttgtggg agagggatag atttttcttc gttcagaaga
     6181 aaaatcaggg agagggtaag aagttactga tgaaagagtt atttccccct ctctctgaaa
     6241 aatatttctg aacgaaatat ttttcggtct ctctcccaca aggggagaga gtaaggctaa
     6301 aaaatgttag atattgtttt atacgaacct gaaattccgc aaaacagcgg aaatattatc
     6361 cgcctttgtg ccaactgtgg ctttcgtttg catatgattg aaccgttcgg ctttggttgg
     6421 gacgataaaa agctccgccg ttcggggctg gattatcacg aatttgtgga tattcaaaaa
     6481 tataaaaatt ttgaagattt ccttgagcgt gctaagccta aacgcttgtt cgccttaacc
     6541 accagagggg agcagaacca cagtgatgtg aaatatgaac ttggcgattt tttaatgttc
     6601 gggccggaaa gcagaggcat tccaaagcca ttattagcgg aaatgccgat gtcgcaaaaa
     6661 atccgcattc c
//
LOCUS       DANJWC010000128         6568 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.128, whole genome shotgun sequence.
ACCESSION   DANJWC010000128 DANJWC010000000
VERSION     DANJWC010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6568
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.128"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..3000
                     /locus_tag="PWX02_001888"
     CDS             <1..3000
                     /locus_tag="PWX02_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDL1729874.1"
                     /translation="GLSQVSYANPLPLGNTTGTNNLAVGEGSFALGTGSQAIGKNAIA
                     TSGNISPQEFKAELENFRNLVNQIEELRTQIAEQEKQGSVNQQLQDTLKSQIAQYDTI
                     LERVREKKAQQAILANSVIPMRKELVNRRNQLTELENNFNTSFLPEKNKQEAYQKFST
                     VIESLDWNKLSATNGVDQLATDLKTKIETDFPNINFSNDQYKDLIKGYRNIQGNLVTA
                     IPKFLNKVNDDFAISNSDRGLYYNYLPYHGIDRWEGSNFNFRTLNGVYYDDNLTSTEL
                     LRKFMIQRDYNTLSELSRAHAGNADGLAKNYHLVVAKMSYENLKKELSKNSDSKFYKL
                     KKLYEAYGEFSNLNEYVSSSFQGNPLELLTVPVLKKSLENQELLPNKLYRKFNIDNQA
                     IAQTTSNITKEEIADLEKTVENFNKFKELIDYSSNAWIFNKEEYRTYMNDNVIPFMEK
                     VEEFTKTLKTLSREDLSEEERTAKTIDSVKLRIYLNEQARNRKNYINGFVPTDWISEI
                     TDQMTKSKNLWLKYEEESKTTLKPYQEETISSLIKDEINKKMAEIDTTTLEIANKERE
                     LEELENQINAFTLTDEEKLAEDVKNDLISKLDEAKAALAKNQQTLKEKHDELLGLNNK
                     LDSSPLVKNGKNALAEGTNAFASGENAIAFGTDSQATGNNAIALGANSKANAESAIAI
                     GKGAQALKEKALALGENAIANGASAIAIGDNIGVSGEKAVGIGSNAIVSGNGAISIGS
                     DNRVSHKNAVAIGSNITQTAENSVNLGNESATTVELTAETAGTTQYAHSEILGEIYKF
                     AASEPAGVVTVGAKGKERRIQNVAAGLVSETSTDAVNGSQLYAVAKAANEGKIGVVRY
                     TNTDGVVRIANHLDGNEVNISNKSGNTRLLTGLSEGLAPTDAVNKAQLDREIGVVNNK
                     ISAVEKEVKKVKGSVSNAIAIASLPQVSVPGKRQLSVATGHTLGTTSVAVGLNGLSDN
                     GRISYKLGTSVSQNSNFAVGAGIGFSW"
     gene            3019..3732
                     /locus_tag="PWX02_001889"
                     /pseudo
     CDS             3019..3732
                     /locus_tag="PWX02_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4041..4481
                     /locus_tag="PWX02_001890"
     CDS             4041..4481
                     /locus_tag="PWX02_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDL1729875.1"
                     /translation="MTACKNGLGFLVLRLFLAYEFFEAGLSKLNGENWFASIQESFPF
                     PFNVLPADLSWAIASGAEILLPILLTLGLFTRFSALGLMILTAVAWYAVHAGNGYNVC
                     NNGYKMALIYLVMLVPLLLQGAGSLSLDFLLQKKSPTKRWLKFL"
     gene            4507..4794
                     /locus_tag="PWX02_001891"
     CDS             4507..4794
                     /locus_tag="PWX02_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729876.1"
                     /translation="MKKATLMALAGALTMATATVNAETTSVKADACMEVKDGKCVKTK
                     ATEGKCGEGKCGATSSEPKATEGKCGEGKCGATAPKAAEGKCGEGKCGASK"
     gene            4895..5818
                     /locus_tag="PWX02_001892"
     CDS             4895..5818
                     /locus_tag="PWX02_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HDL1729877.1"
                     /translation="MLKFTGAGLGYRRDLADGFLSLTDNNIINFMEVAPENWLKMGGQ
                     ARYNFDKVAEKIPVIIHGLSLSLGGQAPLDMTLLKGIKNMQQQYNSFFFSDHLSYCEC
                     EGHLYDLLPMPFTDEAVHHVANRIKQVQDYLGMQISLENTSYYLHSPTSTMNEVEFLN
                     AIAQEADCGIHLDVNNIYVNGVNHGLLDPFVFIDQVDKSRVNYIHIAGHDEDHGAAED
                     VKMTEGESFNMVKGELRHLPTLLVDTHGEAVQKTVWDLLEYTYEHLPHIPPTLLERDF
                     NFPPFQELISEVQHIMSLQKKYAKTRLKNAA"
     gene            5808..6548
                     /locus_tag="PWX02_001893"
     CDS             5808..6548
                     /locus_tag="PWX02_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HDL1729878.1"
                     /translation="MPLKLVEEHKPLLKEIQAEFANAIRHNDVKLMSGNISPQRLGVY
                     SRLVRNNTLGFIDRCYVQLPQHLSPEEWVAVKEKFIREGKAHSPFFQDIAGEFLNFCR
                     ENGCMPPHLLELMDFENAQLLAEVSMATVPSEFEWDNDTIMQFSGAAELRQYAVNFIR
                     SEFKQFDFEPTNVLIWRDAEFGIYYNRLEELDFFLLSSLQEGANSLNGLLAELSEFTE
                     QNLELKALLRQLWEKWVEAEVIYPVYPE"
BASE COUNT         2247 a         1178 c         1295 g         1848 t
ORIGIN      
        1 gggcttagcc aagtctctta tgccaatcca cttcctttag gcaacaccac tggcaccaat
       61 aaccttgctg taggtgaagg tagttttgcg ttaggcacag gttctcaagc aatcggcaag
      121 aatgccattg caaccagtgg taatatctcc ccccaggaat tcaaggcgga gttagaaaac
      181 ttccgtaatt tagttaatca aattgaagag ttacgtactc aaattgctga acaagaaaaa
      241 caaggctctg ttaatcaaca attacaagat actttaaaga gtcagattgc tcaatatgac
      301 actattttag agcgtgtacg tgaaaaaaaa gcacaacaag caatattagc caatagcgtt
      361 ataccaatgc ggaaagaatt agttaataga agaaatcagt taacagaatt ggaaaataat
      421 tttaatacta gttttttacc agaaaaaaat aaacaagaag catatcaaaa attttctaca
      481 gtaatagaat cattagactg gaataaatta tcagcaacta atggggtaga tcaattagca
      541 acagacttaa aaactaaaat tgaaactgat ttccctaata ttaatttttc aaatgaccaa
      601 tataaagatc tcattaaagg ttatcgcaat attcaaggta acttagtaac agccattcct
      661 aaattcctga ataaagttaa tgatgatttt gctataagta atagtgatag aggcttatat
      721 tataactacc ttccttatca tggtattgat cgttgggagg gttctaattt taactttcga
      781 acactaaatg gagtttatta tgatgataat ctaacttcaa cagaactcct gcgaaaattt
      841 atgattcaaa gagattataa cactctttct gaactaagtc gtgctcacgc aggaaatgct
      901 gatggtctgg ctaaaaatta ccatcttgta gttgcgaaaa tgtcctatga aaacctgaaa
      961 aaagaattat ccaagaactc cgactctaaa ttttataaac tgaaaaagct atatgaagcc
     1021 tatggtgaat tctcaaattt aaatgaatat gtttcaagca gtttccaagg aaatccactt
     1081 gaattattaa cagtacctgt gcttaaaaaa agtttagaaa accaagagtt actccctaac
     1141 aagctgtata ggaaatttaa tattgacaac caggctattg cacagaccac atcaaacatt
     1201 actaaagaag aaattgctga tttagaaaaa actgtagaaa atttcaataa gttcaaagag
     1261 ttaattgatt atagtagcaa tgcgtggatc tttaataaag aagaatatcg cacttatatg
     1321 aatgataatg taattccttt tatggagaag gtagaggaat ttactaaaac attaaaaact
     1381 ttatctagag aagacttaag tgaagaggaa cgtacagcca aaacaattga ctccgttaaa
     1441 ttaagaatat atctcaatga acaagcacga aatcgtaaaa attatattaa tggatttgta
     1501 cctaccgatt ggatttctga aattacagat caaatgacaa aatccaaaaa tctatggtta
     1561 aaatatgaag aggaatccaa aacaacatta aaaccatacc aagaagaaac aatatctagt
     1621 cttataaaag atgaaattaa taaaaaaatg gctgaaatag atactacaac actagagatt
     1681 gctaataaag aaagggagct cgaggagctt gaaaaccaaa ttaatgcttt cactttaact
     1741 gatgaagaaa aacttgcgga agatgtgaaa aatgatttaa tctcaaagct tgatgaggct
     1801 aaagcagcct tagcaaaaaa ccagcaaacg ctcaaagaaa agcatgatga attactaggg
     1861 ctaaataaca aattagatag ctcaccactt gttaaaaacg gtaaaaatgc acttgcagaa
     1921 ggcacgaatg cctttgcttc tggtgaaaat gccatcgcat tcggcacgga ttcacaagct
     1981 acgggtaata acgctatcgc actaggtgca aattcaaaag caaacgcaga aagtgcgatt
     2041 gcaattggta aaggtgcaca agcattaaaa gaaaaagccc ttgctttagg cgaaaatgcc
     2101 attgccaacg gggcatctgc tattgcgatt ggtgataata taggtgtttc tggtgaaaaa
     2161 gccgtaggta ttggttcaaa tgctattgta tctggcaatg gggcaataag tattggttct
     2221 gataatcgcg tttcgcataa aaatgcagta gctattggtt caaatattac ccaaacggct
     2281 gaaaactcag taaatttagg caatgagtca gcaacaaccg tagaacttac cgctgaaaca
     2341 gctggcacaa cgcaatacgc acattccgaa attttaggtg agatctataa atttgccgcc
     2401 tcagaacctg ctggtgtcgt gactgttggg gcaaaaggta aagagcgtcg tattcaaaat
     2461 gttgctgcag gtttagtttc tgaaaccagt actgatgcag taaatggtag ccaattatat
     2521 gccgtagcca aagcagccaa tgaaggtaaa atcggtgttg tgagatatac aaatacagat
     2581 ggtgtagtaa ggattgctaa ccatttagat ggtaatgaag tcaatatcag caataagtcc
     2641 ggcaatactc gcctcttaac tggactttct gaaggtcttg caccaacaga tgctgtaaat
     2701 aaagcacagc ttgatcgtga aataggtgta gtaaataaca aaatcagtgc tgtagaaaaa
     2761 gaggtaaaaa aagtgaaagg ttcggtatca aatgctatcg caatagcaag cttacctcaa
     2821 gtatctgtac caggaaaacg tcaactctct gtcgctacag gccataccct tggtacaact
     2881 tcagttgcag taggtttaaa tggccttagt gataacggta gaatatctta caagctaggc
     2941 acatctgtat cgcaaaacag taattttgcg gttggtgctg gtattggttt ctcatggtaa
     3001 ggaaacataa tgtattgtat gactaaaaaa atgaattctc attttgtaaa ggcaaacaaa
     3061 atacttgctg ctatgttact tttagctcca ttaggtttct cttatgctga tgaggcgagc
     3121 ttaacaaaat accagcaagc acgtattgcg gtaacttata aaggtaacgt tggtgatctc
     3181 tctcaacaac tcgctaagaa gttaggaatt ggttattatg ccatacagca taattcatct
     3241 attcaagtaa agttgaaaca gaccgataac gataccttgc aacatttgct cgatcaaata
     3301 aaccagcaac ttaaacagca aaatagtaac caacaattac gctttgatat gctagatgat
     3361 aaagtagtgt tggctttagt tggcaacaat gtcagtttag ctccaactca atttattggg
     3421 gatgtggtct atgataataa agctctagtt caagaagaga aagagcgtaa tattatctct
     3481 gtacctcctc aatcaaataa aggatcatat agcgaggtta gcagtgctga agaaaaaaag
     3541 ctcggtgata ttttagcatt gagtcaagat acgaaattaa ttagtaaata tgctaaacga
     3601 aaagtgccta cctataatgt ggttaaccaa cacgcattag ggttagaaaa tatccgtagt
     3661 actaaaatca gcaccttctt aatttttaaa gatggcattg atatgagtaa atataaagtt
     3721 gaaggtcaat ttcaggatat cgctatcgta ggtaacgtag ttggaatttt acatcgtcag
     3781 aaagtaccgc cgaaagaaat tttaattaca gctcctgata aacagcaata taaactagct
     3841 aaagctaatt aaataccctt gcaaaaaaac aaataggtta aattttgcac tgaatttaac
     3901 ctatttttat ttatctataa aaacaggtta taatttttca cacatttttt gtaataaacg
     3961 tatacttatt ctgtctaata accacatttt tagttaggag catttttatg tcatcatttt
     4021 caactcagtc agcttgctca atgacagctt gtaaaaacgg cttgggcttt cttgtattac
     4081 gtctatttct tgcttatgaa ttttttgaag cagggttatc aaaactaaac ggtgaaaatt
     4141 ggtttgcctc aattcaagag agcttcccat tcccctttaa cgtactacca gccgatctta
     4201 gctgggctat tgctagcgga gcggaaatct tactgcctat tttactcact ttgggtctgt
     4261 tcacccgctt cagtgcgtta ggattaatga tcttaacagc ggtagcgtgg tatgctgttc
     4321 acgctggcaa tggctacaat gtctgcaaca atggctataa aatggcactc atttatttag
     4381 tgatgttagt accactttta ttacaaggtg ctggctcgct ctctctcgat tttctgttac
     4441 agaaaaaatc cccaaccaaa cgctggttga aattccttta atcccttaat ttatggagaa
     4501 tttttaatga aaaaagcaac tttaatggca ttagctggtg cattaactat ggcaaccgca
     4561 actgtaaacg ctgaaactac atcagtgaaa gcagatgctt gtatggaagt aaaagatggc
     4621 aaatgtgtaa aaaccaaagc tacagaaggt aaatgtggcg aaggcaaatg tggagcaacc
     4681 tcttctgagc caaaagcaac cgaaggtaaa tgcggcgaag gcaaatgtgg agcaacggca
     4741 ccaaaagcgg cagaaggtaa atgcggtgaa ggcaagtgtg gagcttctaa gtaagtagcg
     4801 aaggtgcgtt ttctaaacgc acctaacctc ttataaacac aatctacaag cggttaaatt
     4861 ccgctgattt tttgcaaatt aaaggaacaa gacgatgtta aaatttactg gtgctggtct
     4921 aggctatcgc cgtgacctag cggacggatt tttatctctc acagataaca atataattaa
     4981 ttttatggaa gtagccccag aaaactggct gaaaatgggc ggtcaggctc ggtataactt
     5041 cgataaagtc gctgaaaaaa tccctgttat tattcacggt ttatccctct ctctcggcgg
     5101 gcaagcgcct ttagatatga cgctactaaa agggatcaag aatatgcagc agcagtataa
     5161 ctcctttttc ttctctgatc atttaagcta ttgcgaatgt gaagggcatt tatatgacct
     5221 gctgccaatg ccgtttaccg atgaagccgt acatcacgtg gcaaatcgca ttaaacaagt
     5281 gcaagattat ttaggtatgc aaatttccct tgaaaatacg tcctactatc tgcattcacc
     5341 aaccagtacg atgaatgagg tagagtttct caatgcaatt gcccaagaag cggattgtgg
     5401 cattcacctt gatgtgaata atatttatgt taatggtgtc aatcacggct tgcttgatcc
     5461 atttgtattt attgatcaag tggataaatc tcgtgtcaat tatattcata ttgcaggcca
     5521 cgatgaagat cacggtgcgg cggaagacgt taaaatgacc gagggtgaat catttaatat
     5581 ggtaaaaggc gaactacgtc atctgccaac tttactggtc gatacccacg gagaagctgt
     5641 acaaaaaact gtgtgggatt tactagagta cacctatgaa cacctaccgc atattccacc
     5701 aacactgtta gaacgggatt tcaacttccc accgtttcaa gaactgatta gtgaagtaca
     5761 gcacattatg agcttacaga aaaaatatgc aaaaacgagg ctaaaaaatg ccgcttaaac
     5821 tcgttgaaga acacaaaccg ttactcaaag agattcaagc cgagtttgcg aatgcaattc
     5881 gccataacga tgtaaagctg atgagtggta atatctctcc acagcggctt ggcgtctatt
     5941 ctcgcttggt tcgcaataat actctcggtt ttatcgaccg ttgttatgta cagctccctc
     6001 aacatctaag tcctgaagaa tgggttgccg ttaaagaaaa atttatacga gaaggtaagg
     6061 ctcattcgcc ctttttccaa gatattgctg gcgaatttct caatttttgc cgagaaaatg
     6121 gttgtatgcc accacacttg cttgagttga tggactttga aaatgctcaa ttactggctg
     6181 aggtatcaat ggcaaccgta ccatcagaat ttgaatggga taacgatact ataatgcagt
     6241 tttctggtgc agctgagctg cgtcaatatg cggtaaactt tatccgtagc gagttcaaac
     6301 agtttgactt tgagccgacc aacgtactga tttggcgaga tgcggaattt ggcatctatt
     6361 acaaccgctt ggaagagctg gatttcttcc tgcttagctc gttgcaagag ggggcaaatt
     6421 ccctgaatgg cttactagca gaactcagcg agtttaccga gcaaaatctg gagctaaaag
     6481 ccttgttacg ccaactttgg gaaaaatggg ttgaagcaga ggtgatttat cctgtctatc
     6541 ctgaatgagg ataaggtatg tgatatgt
//
LOCUS       DANJWC010000129         6550 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.129, whole genome shotgun sequence.
ACCESSION   DANJWC010000129 DANJWC010000000
VERSION     DANJWC010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6550
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.129"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            125..622
                     /gene="tpx"
                     /locus_tag="PWX02_001894"
     CDS             125..622
                     /gene="tpx"
                     /locus_tag="PWX02_001894"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005578656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDL1729879.1"
                     /translation="MSKVTLAGSPIDVAGTFPQKGDVVADFTLVNSDLSDVSLSDFDG
                     KRKVLNIFPSIDTGICATSVRVFNQKAADVANTVVLCISADLPFAQARFCGAEGIENA
                     KVLSSFRHRDLHNKLGVDIISGPLAGLTSRSVIVLDENNKVLHSELVPEIKQEPDYEA
                     ALAVL"
     gene            complement(707..2332)
                     /gene="yidC"
                     /locus_tag="PWX02_001895"
     CDS             complement(707..2332)
                     /gene="yidC"
                     /locus_tag="PWX02_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL1729880.1"
                     /translation="MNNRGLLLMGLFLVSILIFTQWQQDFDPEIQAQKQAQVAAQTQQ
                     AGDLPSASNANNPIADSITQGKTITVESDVLRLTIDTLGGDVIGSDLLKHNAELHSNT
                     PFKLLQQDGDKIYVAQSGLVGKNGIDTNAGRPQYAVTADSFKLAEGQNEISVPLTFEK
                     EGVVYTKTFTLKRGSYDVGVNFNIKNNTAQAIEVQPYGQLKHSIVESSGNLTMPTYTG
                     GAYSSSETNYKKYSFQDMEKANLSVDTKAGWIAVLQHYFVSAWVPNQDADNSLYSRTN
                     NGVATIGYRGPLTTIAPNSEANLNSKLWTGSKDQKEMGESANHLDLTVDYGWAWFIAK
                     PLFWLLTAIQKIVSNWGLAIIGVTIVVKTILYPLTKAQYTSMAKMRMLQPRITEMRER
                     FGDDRQRMSQEMMKLYREEKVNPMGGCLPILIQMPIFIALYWTFMEAVELRHAPFFGW
                     IQDLSAQDPYYILPLLMGGSMFLLQKMSPTPVADPVQQKVMTFMPVIFTVFFLWFPSG
                     LVLYWLTSNLITIAQQWLIYRNLEKKGLHSRKK"
     gene            complement(2485..2910)
                     /locus_tag="PWX02_001896"
     CDS             complement(2485..2910)
                     /locus_tag="PWX02_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HDL1729881.1"
                     /translation="MTIWKQTATCEQLNTLSQKSAVSHLGIEFTAIGEDWIEAQLQVD
                     ERTQQPFGVLHGGVSAALAETTANAGALMVCEPHQIAVGMELNISHLKSIPAGTKAIA
                     KATPLKLGREVQVWNIDIKDEQGNLCAVARLSTKTLDKR"
     gene            complement(3239..6331)
                     /locus_tag="PWX02_001897"
     CDS             complement(3239..6331)
                     /locus_tag="PWX02_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDL1729882.1"
                     /translation="MIPRLSEVPQLDKATQSYLTELVNQHFSGEIASNYADRLSLATD
                     NSVYQQIPQAILFPKSVSDVVILTKLAQKPEYQHLTFTPRGGGTGTNGQSLNNNIIVD
                     LSRHMNQILELNVEERWVRVQAGVVKDQLNQFLKPHGLFFSPELSTSNRATLGGMINT
                     DASGQGSLQYGKTSDHILAIKSVLINGDILETQAVKSADFFAHIEGLNLSALGKTLHR
                     EIFNRCKTLRPKVLNELPQLNRFITGYDLKNVFNHGESEFNLTRILTGSEGSLAFICE
                     AKLNLLPIPKYRTLINIKYNSFDAALRSAPFMLEAKALSVETVDSKVLNLAKQDIVWH
                     SVSDLLTEDPANPILGINIVEYASSDQTQIETQVKALCQSLDQKMANNEAGIIGYQLT
                     SDVNSIEKIYAMRKKAVGLLGNAKGWAKPIAFVEDTAVPPENLADYIVEFRALLDRYN
                     LPYGMFGHVDAGVLHVRPALDLCDKAQVETFKQISDQVVELTAKYGGLIWGEHGKGMR
                     SYYGERFFGETLWQELRYIKTLFDPYNRLNPGKICTPLESETSLYPIDSQMRADFDRQ
                     IPIQVREEFKGAMNCNGNGLCFNFDVHSTMCPSMKVSGNRLYSPKGRATLVREWLRLL
                     TEQNVSPDDLIFKPRKQAVKLADFVAKIRNTLNKNKEYDFSHEVKAAMDTCLACKACA
                     SQCPIKIDVPTFRSQFNELYHSRYLRPLKDYVVSNLEFIAPLMAKQPRFFNFFSTSTF
                     AEKAANKTIGMTYLPALSVPNLQHQLVEVGYQGESLESLEQQATSGDFSAKNCKTLFI
                     VQDPFTSYYDAKVVADFIALIKKLGFKPIVLPFKPSGKAQHVKGFLHKFAKTAKNQAD
                     FLNRVAKLGLPMVAVDPALTLLYREEYKEILKEARGDFKVLLAHEWLREVIKNGELEN
                     CKKSANSDRLPWYLFAHCTESTALPSSMKEWQAIFAHFGETLLNENVGCCGMAGTFGH
                     ETKHVEMSKAIYQQSWQIKLKNKPLERCLATGYSCRSQVKRFEQQGIKHPIQALLDVI
                     "
BASE COUNT         1771 a         1296 c         1443 g         2040 t
ORIGIN      
        1 attgtctagg agtatagcaa aataaggtta ttttctaaag gctatccaca ttaaattgat
       61 agtttcctat tttgcaaaaa atcggtatat ttaaaccgct tgtttatttg ataaggagaa
      121 aaaaatgagt aaagtgactt tagcaggaag cccgattgat gttgcaggca catttccaca
      181 aaaaggcgat gtggttgccg attttacctt agtcaacagt gatttaagtg atgtttcgct
      241 ttccgatttt gatggcaaac gcaaagtgtt aaatattttc ccaagtattg acaccggcat
      301 ttgtgccact tctgtgcgtg tgtttaatca aaaagcggct gatgtagcga atacggttgt
      361 cctttgcatt tcagcggatt taccttttgc ccaagcacgt ttctgtggag cagaaggaat
      421 tgaaaatgcc aaagtattat ccagtttccg tcatcgtgat ttacacaaca aactgggggt
      481 agatattatt tcaggtccgt tggcaggctt aacttcacgt tcagtaattg tgttagatga
      541 aaacaacaag gtattacaca gcgaattagt gccggaaatt aaacaagagc cggattatga
      601 ggcggcgtta gcggtattat aatttgcaga attatgctgg aaaataaccg cttgtaaagc
      661 aacaaaaagc ccgttgattc aattcaacgg gctttgtttt tagtgattat ttcttacgag
      721 agtgtaagcc ttttttctca aggttacggt aaatcaacca ttgctgtgca atcgtgatta
      781 agtttgaggt taaccagtaa agcactaagc ctgatgggaa ccataagaag aacacggtaa
      841 agataaccgg cataaaggtc attacttttt gttgtaccgg gtcagctacc ggtgttggcg
      901 acattttttg taacaagaac atcgaaccac ccattaataa aggcaaaatg tagtatgggt
      961 cttgtgccga taagtcttga atccagccga agaaaggtgc gtggcgtaat tctaccgctt
     1021 ccataaatgt ccaatataaa gcaatgaaaa tcggcatttg gattaaaatt ggtaagcagc
     1081 cacccattgg gttcactttt tcttctctgt agagtttcat catctcttgg ctcatacgtt
     1141 ggcggtcgtc gccgaaacgt tcacgcattt cagtaatgcg aggttgtaac atacgcattt
     1201 ttgccatcga ggtgtattgt gctttggtta atgggtaaag aatggttttc actacaatag
     1261 tgacaccgat aattgccaag ccccagttgc ttacgatttt ttgaattgcg gttagtaacc
     1321 agaaaagcgg tttagcgata aaccacgccc agccgtaatc tacggttaag tctaggtgat
     1381 ttgctgattc gcccatttct ttctgatctt tagaacctgt ccaaagtttg ctatttaaat
     1441 tagcctctga atttggcgca atcgtagtta aagggccacg gtaaccgata gttgctaccc
     1501 cattattggt gcgtgaataa aggctgttat ccgcatcttg gtttggcacc caagcagaca
     1561 cgaaatagtg ctgtaatacc gcaatccaac ccgctttggt gtccacgctt aagttggctt
     1621 tttccatatc ttggaaactg tattttttgt agttggtttc tgaagaagaa taagccccgc
     1681 cggtgtaagt cggcatggtt aaattgccgg aactttcaac tatagagtgt tttaattgcc
     1741 cgtaaggctg aacttcaatc gcttgtgcag tattattttt gatattgaag tttaccccaa
     1801 catcgtagct accacgtttt agcgtgaaag ttttggtgta aaccacaccc tctttttcaa
     1861 aggttaatgg tacagaaatt tcattttgcc cttcggctaa tttgaagcta tctgccgtta
     1921 ccgcatattg tgggcgaccg gcattggtat caatcccgtt tttacccact aaaccgcttt
     1981 gagcaacgta gattttgtcg ccgtcttgct gtaatagttt aaatggcgtg ttagagtgta
     2041 attcagcatt gtgttttaac aaatctgagc cgatgacatc accgcctaag gtatcaatag
     2101 tcagacgtaa tacatcgctt tccacggtaa tggttttacc ttgcgtgatg ctatctgcga
     2161 ttggattatt ggcattggaa gcagacggaa gatcacccgc ttgttgggtt tgtgctgcca
     2221 cttgagcttg tttttgtgct tgaatttcag gatcaaaatc ttgttgccat tgtgtaaaaa
     2281 tcaaaataga cacaagaaat aaacccatta gcagtaagcc acgattattc atgttggaat
     2341 tctcttgtta gtaatggata aaaagaaagg tattttataa taggttagct tgttttccaa
     2401 acgcctttta tgaacaagcg gtcggatttt ccaagttttt tgcaaaattt tcggagaatt
     2461 tgaccgcttg tggagaataa gaaactaacg tttatctaag gttttggtgg aaaggcgagc
     2521 aactgcacac aggttacctt gttcgtcttt aatgtcgata ttccaaacct gtacttcacg
     2581 ccctaatttc agcggtgtag ctttggcaat cgcttttgtg cctgccggaa tcgattttaa
     2641 atggctgatg tttagctcca ttcccaccgc aatttgatgg ggttcacaca ccattaacgc
     2701 ccctgcattg gcagtagttt ccgcgagtgc tgccgataca ccgccgtgca atacgccaaa
     2761 cggctgttga gtgcgttcgt ccacctgaag ctgtgcttca atccaatctt caccgatagc
     2821 ggtaaattca atccctaagt gtgacaccgc cgatttttgg ctgagtgtgt ttaattgttc
     2881 acaagttgct gtttgcttcc aaatggtcat tattatttcc tgtctaataa aaaagctgtc
     2941 tatttagaca gctttcatta tctctttgag cgaaacatac tacgccaata gtacattgtt
     3001 tggtcgccca tattacctta agccatatag cactaaggag gtgatttatg tgagaaaatc
     3061 agaagttttc tttctggttg cgttgctaat attattagca acttccgcta gcattaaagt
     3121 actcaccata cttgatgtgc tagtttggtt attcgctaga taacctaaaa tcagacagag
     3181 gcaggctggt tacctgcctc gctttttatt ctaagtcctt ttaggttaca tttcaacctt
     3241 aaatcacgtc cagcaacgct tgaattgggt gtttaatgcc ttgttgttca aaacgtttga
     3301 cttgcgagcg gcaggagtag ccggttgcta ggcaacgctc aagcggttta ttttttaatt
     3361 taatttgcca tgattgctga taaatcgctt tcgacatttc aacgtgtttg gtttcgtgtc
     3421 cgaacgtgcc tgccattcca cagcaaccga cattttcatt gagtaaggtt tcaccaaaat
     3481 gggcgaaaat agcttgccac tctttcattg agcttggcaa ggcggtggat tcggtgcagt
     3541 gagcaaataa ataccacggc aagcggtcgg aatttgccga ttttttgcaa ttttccagct
     3601 cgccgttttt aatcacttcc cttaaccatt cgtgtgccaa taagactttg aaatcccccc
     3661 gagcttcttt gaggatctct ttgtattctt cacgataaag caaagttaag gcaggatcga
     3721 cagccaccat cggtagcccc aatttcgcca cacggtttaa aaaatcggct tggtttttcg
     3781 ctgttttcgc aaatttatgc aaaaagcctt taacgtgttg tgctttacca cttggcttga
     3841 atggcaacac aatcggctta aagcctaatt ttttaatgag agcgataaag tctgccacca
     3901 ctttggcatc gtaataagag gtaaacggat cttgcacaat aaacaaggtt ttgcaatttt
     3961 ttgcagaaaa atcaccgctt gtagcttgtt gttcaaggct ttctaagctc tcgccttggt
     4021 agccgacttc caccaactga tgttgcagat ttggcactga caatgccggc aaataggtca
     4081 ttccaatggt tttgtttgca gctttttcgg caaaagttga ggtgctgaaa aagttaaaaa
     4141 aacgtggttg tttcgccatt aaaggggcga taaattctaa gtttgagacc acataatctt
     4201 tcagtggtcg caaatagcgg ctgtggtaaa gctcgttaaa ttgcgatctg aacgtcggta
     4261 cgtcaatttt gattgggcat tggctggcac aggctttgca ggcaaggcag gtgtccatcg
     4321 ccgctttgac ttcgtgtgaa aaatcatact ctttgttttt attgagcgtg ttgcgaattt
     4381 ttgcaacaaa atcagctaat ttgaccgctt gtttgcgagg tttgaagatc aaatcgtccg
     4441 gcgaaacgtt ttgttcggta agtaatctga gccattcacg cacaagagta gcccgccctt
     4501 tcggtgagta aagacggttg cccgagactt tcattgaagg gcacatcgtg ctgtgtacat
     4561 caaagttaaa acatagcccg ttgccgttac aattcattgc tcctttaaat tcttctcgca
     4621 cttggattgg aatttggcgg tcaaaatcgg ctcgcatttg tgagtcaatc gggtagagcg
     4681 aggtttcgct ttctaatggt gtacaaattt tacctgggtt caagcgattg tacgggtcaa
     4741 ataaggtttt gatgtaacgg agttcttgcc acaaagtttc gccaaagaaa cgttcgccgt
     4801 agtaagaacg catacctttg ccgtgttcgc cccaaatcaa gccaccgtat ttggcggtga
     4861 gttcgactac ttggtcggaa atttgcttaa aggtttccac ttgggctttg tcgcataagt
     4921 caagagcagg gcgaacgtgt agcacgccgg catcaacgtg gccgaacatt ccgtaaggca
     4981 gattatagcg atctaataaa gctcgaaatt ccacgatgta atcggcaagg ttttccggtg
     5041 gcacagcggt gtcttctaca aaggcgatcg gttttgccca gcctttggca ttgcctagca
     5101 ggcctacggc ttttttccgc attgcgtaga ttttctcgat agaatttaca tcactcgtga
     5161 gttggtagcc gataatcccg gcttcattat ttgccatttt ttgatcaagg ctttggcaga
     5221 gtgctttcac ttgggtttca atttgagtct gatcgcttga ggcatattca acaatgttaa
     5281 tccctaaaat cgggttggct gggtcttcgg ttaataaatc tgacaccgaa tgccagacga
     5341 tatcttgctt ggcaagattg agcactttgg aatcaaccgt ttccaccgat aaggcttttg
     5401 cctctaacat aaaaggagcg gagcgtaagg cggcatcaaa ggaattatat ttaatattaa
     5461 ttaaggtgcg atatttggga atcggcagta aattcagttt ggcttcgcaa ataaaggcaa
     5521 gcgaaccttc agaaccggtt aaaatacggg taaggttaaa ttcgctttca ccatggttaa
     5581 acacattttt taaatcatag ccggtaataa aacggttaag ctgaggcagt tcgttcagca
     5641 ctttggggcg taatgttttg caacggttga aaatttcacg gtgcaaggtt ttgccgagag
     5701 cggagagatt taacccttca atatgtgcaa aaaaatcggc agatttgacc gcttgtgtct
     5761 ctaaaatatc gccgttaata agcacagatt tgatggcaag aatatgatca gatgttttgc
     5821 catattgcaa agaaccttgc ccagaggcat cggtgttgat cattccgcct aaagtcgctc
     5881 ggttactggt agaaagttcg ggggaaaaga aaagtccatg tggttttaaa aattgattga
     5941 gctggtcttt caccacgcca gcctgcactc gaacccaacg ctcttccaca ttcagttcca
     6001 aaatctgatt catatggcgg gagagatcaa caataatatt attgttaaga gattgcccgt
     6061 tagtgcctgt gccaccgcct cgtggagtaa aagttaaatg ttgatattct ggcttttggg
     6121 caagtttggt tagaattacg acatcagaaa cggatttcgg aaacagaata gcttgcggaa
     6181 tttgctgata cacgctgttg tctgttgcca gtgaaagcct atctgcataa tttgatgcaa
     6241 tttccccact aaaatgctga ttgacgagtt cagttaaata ggattgggtg gctttatcca
     6301 gttgcggtac ttcagagagt ctaggtatca taagctgtgt ttggtttttt ataataaaag
     6361 cactaaattt tgcttatctt agcaattttt ctaaaaattg ggaatggaat atcaatttga
     6421 ttaaatagtg ttcgaaaaat ggtttttggt taaaatatag acaaattatt acgtttctat
     6481 acatttcatc aggaaaaaac gattgatttt tcgaaaatga aaccacataa tgacaatatt
     6541 cttgctcaat
//
LOCUS       DANJWC010000130         6493 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.130, whole genome shotgun sequence.
ACCESSION   DANJWC010000130 DANJWC010000000
VERSION     DANJWC010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6493
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.130"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(109..1308)
                     /locus_tag="PWX02_001898"
     CDS             complement(109..1308)
                     /locus_tag="PWX02_001898"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HDL1729883.1"
                     /translation="MFKFSTVEEAIQAIAQGKIILVSDDADRENEGDFICAAEFATPE
                     NINFMAKYGKGLICTPISTEIAKKLNFHPMVLENEDNHQTAFTVSVDHIQTGTGISAF
                     ERSLTCLKMLDENAKPEDFRRPGHVFPLVAKDGGVLVRNGHTEATVDLARLAGLKPAG
                     LCCEIMAEDGTMMQMPELQAFAQAHNMPFITIQQLQEYRRKHDSLVNVVSVVKMPTKY
                     GEFKAHSFVETISGKEHVALVKGDIGDGENVLCRIHSECLTGDAFGSQRCDCGQQFAA
                     AMNQVEQEGRGVVLYLRQEGRGIGLINKLRAYELQDRGMDTVEANLALGFKDDEREYY
                     IAAQMFEKLGVKSIRLLTNNPAKIEGLIEQGLNVVAREPIMVEPNEHDLEYLKVKQHK
                     MRHMFNF"
     gene            complement(1327..1974)
                     /gene="ribE"
                     /locus_tag="PWX02_001899"
     CDS             complement(1327..1974)
                     /gene="ribE"
                     /locus_tag="PWX02_001899"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDL1729884.1"
                     /translation="MFTGIIEEVGQIAQIKKQGEFVVVTVKAKKILTDVHLGDSIAVN
                     GVCLTVTSFTQEQFTADVMSETLKRTSLGELSINSSVNLERAMAANGRFGGHIVSGHI
                     DGTGVVAEITPADNSTWYRIKAEPKLMRYIIEKGSITIDGISLTVVDVDAESFRVSII
                     PHTIKETNLGTKKIGSLVNLENDIVGKYIEQFLLKKEPENPQSKITADFLKNAGF"
     gene            complement(2156..3253)
                     /gene="ribD"
                     /locus_tag="PWX02_001900"
     CDS             complement(2156..3253)
                     /gene="ribD"
                     /locus_tag="PWX02_001900"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005708727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDL1729885.1"
                     /translation="MTDVQYMAYAITLAEKARGWTSPNPLVGCVIVKNGNIIAEGYHQ
                     KAGSWHAERNAILNCEEDLTGATAYVTLEPCCHHGRTPPCSELLIERGIKKVFIGSRD
                     PNPLVSGKGAAQLRAAGIEVVEDFMRTECDALNPIFFHYIQTKRPYVLLKYGMTADGK
                     IATYTGNSKWITGEAARANVQKSRHEYSAIMVGVETVLADDPMLNSRMENAKQPVRIV
                     CDSQLRTPLDSQLVQTARQYRTIIATLNNDAKKQAEYTACGVEILVTGERDKRVDLAD
                     LLQKLGELGIDSLLIEGGSQLNFSVLEQGLVNRVHCYIAPKLIGGKAAKTPIGGKGIG
                     ELAQAVKLRLKSTELLGEDILLDYEVDLLRN"
     regulatory      complement(3429..3547)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            3751..6153
                     /gene="lon"
                     /locus_tag="PWX02_001901"
     CDS             3751..6153
                     /gene="lon"
                     /locus_tag="PWX02_001901"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDL1729886.1"
                     /translation="MAPRKKKAIELPLLPLRDVVVFPYMVMPLFVGREKSIQALRSAM
                     DSNKQLFLVTQQDPNKEEPNAEDMYGVGVIANIIQMLNLPDGTVKVLVEGQTRAKIEQ
                     IHDDENGFWAAIQPIYSEYDDENEELKAIAKTTLTEFENYVKNNKKIPAEIIAKLQKI
                     TLEDRLADTIASNLIAPVKKKQELLEQPNLIARFEALLIAMATEMDTLETETRIRNRV
                     KQQMEKNQRDYYLNEQIKAIQKELGNGDDVEQSELDKLKEKIDAAKLPTEVKDKLNTE
                     FKKLKAMPQSSSEATVVRGYIDWILQMPWHKKSAVKKDLAQAQEILDKDHYGLERVKE
                     RIVEYLAVQSRLNKLKGPILCLVGPPGVGKTSLGQSIANATGRKYVRMALGGVRDEAE
                     IRGHRRTYIGSMPGSLMMKMAKVGVRNPLFLLDEIDKMAQDMRGDPASALLEVLDPEQ
                     NKAFNDHYLEVDYDLSDVMFVATSNSMNIPPALLDRMEVIRLSGYTEDEKMHIARGHL
                     LAKQQENNGLKEGELTIEDSAILSIIRYYTREAGVRSLEREIAKICRKAVKALVLDKK
                     LKSITVSEKNIEEYLGVKRFDYGKMDSQNRIGEVTGLAWTEVGGDLLTIETASVAGKG
                     KFSYTGSLGDVMKESIQAAMVVVRSRAEKLGIAEDFHEKRDIHVHVPDGATPKDGPSA
                     GIAMCTALISSLTGNPVRKEVAMTGEISLRGKVLPIGGLKEKLLAAHRGGITTVIIPK
                     ENEKDLEEIPENAKAALDIHAVETIDEVLALALENPPMGIEILSKAEIKVKKSRAKAT
                     VQ"
     gene            complement(6287..>6493)
                     /locus_tag="PWX02_001902"
     CDS             complement(6287..>6493)
                     /locus_tag="PWX02_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249287.1"
                     /note="participates in controlling several genes involved
                     in isoleucine and valine biosynthesis; activates the
                     transcription of the ilvC gene in the presence of
                     acetolactate or acetohydroxybutyrate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HDL1729887.1"
                     /translation="IVPMVALGFGVALLPDIVIKHSPMNNQVSYVNLPSPITPFELGI
                     CVQQKRLQEPIINAFWQLLSDNSA"
BASE COUNT         1880 a         1440 c         1354 g         1819 t
ORIGIN      
        1 gagaggggaa attgcaaact ttttctaaaa aatataccct ctctctgaaa aatacttctg
       61 aacgaagtat ttttctgtct ctctcccaca aggggagaga gtattggatt agaaattaaa
      121 catatgtcgc attttgtgct gtttaacttt aaggtattcc aaatcgtgtt cgtttggctc
      181 aaccataatc ggctcacgag ccactacgtt taaaccttgc tcaatgagtc cttcaatttt
      241 tgccggatta ttggttaaca aacgaatcga ttttacgcct aatttttcaa acatttgagc
      301 ggcgatataa tattcacgct catcatcttt gaaacctaat gccaggttgg cttcaaccgt
      361 gtccattcct ctatcttgca attcgtaagc acgcagttta ttaattaaac cgatgccacg
      421 tccttcttgg cgtaaataaa gcaccacgcc tctgccctct tgctccactt ggttcattgc
      481 cgctgcgaac tgctgaccac aatcgcaacg ttgtgagcca aaggcatcgc cggttaaaca
      541 ttcggaatgg atacggcata atacattttc gccatcacca atatcacctt taactaaagc
      601 gacgtgttct ttgcctgaaa tagtttcaac gaaactgtgt gctttgaact caccgtattt
      661 ggtcggcatt tttaccaccg aaaccacatt caccaagctg tcgtgttttc tacgatactc
      721 ttgtaactgc tgaatagtaa taaacggcat attgtgtgct tgtgcaaatg cctgtaattc
      781 cggcatctgc atcatcgtgc cgtcttctgc cataatttca cagcaaagcc ctgccggttt
      841 caagcccgcc aaacgagcta aatccacagt tgcttcggta tgaccgttac gcactaatac
      901 gcctccgtct tttgccacta atgggaaaac atgccccgga cgtctaaaat cttccggctt
      961 agcattttca tcaagcattt tcaggcaagt taatgaacgc tcaaaagcgg aaataccggt
     1021 gccggtttga atatggtcaa cagaaacggt aaatgcggtt tgatgattat cttcgttctc
     1081 tagcaccatc gggtgaaaat ttaatttttt ggcaatttct gtcgaaatcg gcgtacaaat
     1141 caacccttta ccatatttcg ccataaagtt gatattttcc ggtgtcgcaa attccgctgc
     1201 acaaatgaag tccccttcat tttcacggtc ggcatcatcg ctgactaaaa taattttccc
     1261 ttgtgcaatt gcttgaattg cttcttctac cgttgaaaac ttaaacatta tttgtccttt
     1321 atttggttaa aaccctgcat ttttaagaaa gtccgcagta attttacttt gcggattttc
     1381 aggctctttt ttcaataaaa actgttcgat atatttgccg acaatgtcat tttctaaatt
     1441 caccaaacta ccgatttttt tcgtaccgag gttggtttcc ttaatggtat gcggaataat
     1501 cgatacccgg aaagattccg catcaacatc caccaccgtt aaactaatgc catcaatagt
     1561 aattgagcct ttttcaatga tgtaacgcat taacttaggt tcggctttaa tgcgatacca
     1621 agttgaatta tctgctggtg taatctctgc aaccacaccc gtgccatcaa tatgacctga
     1681 cacaatatgc ccaccaaaac gcccgtttgc cgccattgcc cgctcaagat taaccgagct
     1741 gttgatactt aattcgccaa gtgatgtacg ttttaaggtt tctgacataa catcagcagt
     1801 aaattgctcc tgcgtaaatg aagtcacggt taaacacacg ccatttaccg caatgctatc
     1861 ccctaaatgt acatcggtca gaatcttttt tgctttaacc gttaccacca caaactcgcc
     1921 ttgtttctta atttgggcta tctggccaac ttcttcgata atgcctgtga acatttaaat
     1981 ttttctccga tttacttcca atttagtctg agaactgctt atcttactat tcccctctcc
     2041 cgtttacggg agagggaaga ttacactcat tttcccctct ctctgcataa aatttctgaa
     2101 cgaaatttta tgctgtctct ctcccgtaaa cgggcgagag taaggaaatt tcaatttaat
     2161 tacgaagcaa gtccacttca taatccaaca aaatatcttc tccgagcaat tcggtggatt
     2221 ttagacgtaa tttaaccgct tgtgctaact cgccaatccc cttgccacca atcggggttt
     2281 ttgccgcttt gccgccaatc agtttcgggg caatatagca atgtactcga ttgactaacc
     2341 cctgttccag cacgctgaaa ttgagttgcg aaccaccttc aatcaataag ctgtcgatcc
     2401 cgagttcgcc cagcttttgc aaaagatcgg ctaaatccac ccgcttgtca cgttcgcctg
     2461 tcactaaaat ttcaacaccg caagcggtat attctgcctg cttttttgca tcattattga
     2521 gcgtagcaat aatggtgcga tattggcgtg cagtttggac tagctggcta tccaatgggg
     2581 tacgcagttg gctgtcacat acaatccgca ccggctgttt agcattttcc attcggctgt
     2641 tgagcattgg gtcatcggca agcaccgttt ccaccccgac cataatcgca ctatattcgt
     2701 ggcggctttt ctgtacatta gctcgggcag cctcgccggt gatccatttc gaattccccg
     2761 tataagtcgc aattttacca tctgccgtca tcccatattt cagcaacaca taagggcgtt
     2821 tggtttgaat gtaatggaag aaaatcgggt tcagcgcatc gcactccgtt cgcataaagt
     2881 cttccacaac ctcaatgccg gcggctctaa gctgtgccgc tcctttgcca gaaacaagtg
     2941 ggttcggatc tcgagagccg ataaacactt ttttgatccc acgttcaatc agcagttcgg
     3001 aacaaggcgg ggttcgcccg tggtggcaac aaggctctaa ggttacataa gcggttgccc
     3061 ccgtcagatc ttcctcgcag tttaaaatcg cattgcgttc agcgtgccaa ctaccggctt
     3121 tttggtgata cccttcggcg ataatgttgc cgtttttcac gatcacacag ccaactagcg
     3181 gattagggct tgtccaaccc cgtgcttttt cggcaagggt gatggcataa gccatatatt
     3241 gaacatctgt catatgcatt ttgataaatt gagggcgtaa tgaaaggatt attgcaatac
     3301 aagcggtcgt ttttagtaaa aaatttgcaa acgccgcctg caaaacaatg aagccttgaa
     3361 atctcacgac tcaaggcttg caaaaaagac acaaaaaatg accgcttgtg cggtattttt
     3421 tgcggttttt gactcttctt tcatccagac tatactgtcg gttttggaat ttcaccaaat
     3481 cctgccgatt gctcggctcg cggactttac cgccgatcgg gaatttcacc ctgccctgaa
     3541 gattaaaata tgcaggggat tttgctcgca ttacgatttg atgtcaagcg ggaaattaag
     3601 ctaattaccc tgattttaac aattgattaa atcgccaaca tctactgttt tgactattcg
     3661 agttttttta agataaatca aattttccct tgtttttccc aaattcgccc tcataatggg
     3721 ggattatatt ttgatagaga gagcaaattt atggcaccaa gaaaaaagaa agcgattgaa
     3781 ttaccactac tcccattgcg tgatgtggtg gtttttcctt atatggttat gccgttattt
     3841 gtaggacgtg aaaaatcaat tcaagcccta cgctcggcga tggattccaa taaacaactg
     3901 tttttagtca ctcagcaaga tccaaataaa gaagagccga atgcagaaga tatgtatggc
     3961 gtaggtgtaa ttgccaacat tattcaaatg ttaaacctgc cggacggcac agttaaagtg
     4021 ctggtagaag gacaaacccg tgccaaaatc gagcaaattc acgatgatga aaacggcttc
     4081 tgggcggcaa ttcagccaat atattcggaa tatgatgacg aaaacgaaga gttaaaagcg
     4141 attgctaaaa ccactttaac cgaatttgaa aattacgtta aaaataacaa gaaaattccg
     4201 gcggaaatta ttgctaaact gcaaaaaatc acgttggaag accgcttggc ggataccatt
     4261 gcctctaact taattgctcc ggtgaagaaa aaacaggaat tattggagca accaaatctg
     4321 attgcccgtt ttgaagcctt gttaattgcg atggcaactg agatggatac gcttgaaacc
     4381 gaaactcgga ttcgtaatcg tgttaaacag caaatggaaa aaaaccaacg tgattactac
     4441 ctcaacgagc aaattaaagc gattcaaaaa gagttaggta atggcgatga cgttgaacaa
     4501 agcgagttag ataagcttaa agagaaaatt gacgctgcaa aattaccgac cgaggtaaaa
     4561 gacaagctga atacggagtt caaaaagctc aaagcaatgc cgcaaagctc ttccgaagct
     4621 accgttgtgc gtggttatat tgactggatt ttacaaatgc cgtggcataa aaaatcagcg
     4681 gtcaaaaaag atttagccca agctcaagag attttggata aagaccacta cggcttagaa
     4741 cgagtgaaag agcggattgt agaatactta gcagtgcaaa gtcgtctaaa taaattaaaa
     4801 ggtccgattt tgtgcttagt tggtccgccg ggggttggta aaacctcatt agggcaatct
     4861 attgccaatg ccacagggcg taaatatgtg cgtatggcgt taggtggcgt gcgtgatgaa
     4921 gcggaaatcc gtgggcatcg ccgtacttac atcggctcaa tgccgggtag cctaatgatg
     4981 aaaatggcga aagtcggcgt gcgtaatccg ctgttcttgt tagatgaaat cgacaaaatg
     5041 gctcaagata tgcgtggcga cccggcttct gctctgcttg aagtgttaga tcctgagcaa
     5101 aataaagcct ttaacgacca ttatttagag gtggattacg atttatccga tgtaatgttt
     5161 gtggcaacct caaactcaat gaacattccg ccagcattgc tcgaccgtat ggaagtgatt
     5221 cgtctttcag gctataccga agatgagaaa atgcacattg ctcgtggtca tttactggct
     5281 aaacagcaag aaaataacgg actaaaagag ggtgagctga ccattgaaga cagtgcaatt
     5341 ttaagtatta tccgctacta cacccgtgaa gcaggcgtgc gtagccttga gcgtgaaatt
     5401 gcgaaaatct gccgtaaagc cgtgaaagct ttagtattgg ataagaaatt aaaatccatt
     5461 acagtttctg agaaaaatat cgaagaatac ttaggcgtaa aacgttttga ctacggcaaa
     5521 atggatagcc aaaaccgcat tggcgaagtg accggtttag cgtggacaga agttggcggt
     5581 gatttattaa ccattgaaac cgcttctgtt gcaggtaaag gcaaattctc ttacaccggt
     5641 tcattaggcg atgtaatgaa agagtctatt caagcggcaa tggtggttgt acgctctcgt
     5701 gcagaaaaat taggcattgc tgaagacttc cacgaaaaac gggatattca cgttcacgtg
     5761 ccggacggtg caacaccgaa agatggtccg agtgcaggga ttgcaatgtg tactgcatta
     5821 atttccagcc taacaggtaa ccctgtgcgt aaagaagtag cgatgaccgg tgaaatcagc
     5881 ttgcgtggca aagtattgcc aatcggcggc ttaaaagaga aattactggc agcccatcgt
     5941 ggtggtatta cgacggtgat tattccgaaa gaaaatgaaa aagacttaga ggaaattccg
     6001 gaaaatgcaa aagcagcgtt ggacattcac gcagtagaaa ccattgacga ggtactagct
     6061 ctggcactag aaaacccgcc aatggggatt gaaatcttgt caaaagccga gatcaaggtg
     6121 aaaaaatcgc gagctaaagc cacagtgcaa taaagtgcaa taatctaaaa caacataagg
     6181 tgcgcagatc acaatcacgc accttatttt ttatctgaaa gttttatcta caagcggtcg
     6241 gttttgctct attttttgca aaaatttagc aaaatctaac cgcttgttat gctgaattat
     6301 cagacaataa ttgccaaaat gcgttgatga tcggctcttg caagcgtttt tgctgaacgc
     6361 aaattcccag ctcaaacgga gtgatcggcg agggtaaatt cacataagaa acttgattat
     6421 tcattgggct atgcttaatc acaatatccg gtagcagtgc gaccccaaag cctagtgcaa
     6481 ccatcggcac aat
//
LOCUS       DANJWC010000131         6477 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.131, whole genome shotgun sequence.
ACCESSION   DANJWC010000131 DANJWC010000000
VERSION     DANJWC010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6477
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.131"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..283)
                     /locus_tag="PWX02_001903"
     CDS             complement(<1..283)
                     /locus_tag="PWX02_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729888.1"
                     /translation="MSTTLNEINITELDAGPEIKSLSLNDAVNSFLNDSACPICGKNC
                     QYWQRLKRSLNRKENQGKGDKVLVKNPSPISIEKNLINAYKEYRKFEEDL"
     gene            complement(317..1603)
                     /gene="purD"
                     /locus_tag="PWX02_001904"
     CDS             complement(317..1603)
                     /gene="purD"
                     /locus_tag="PWX02_001904"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012771675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDL1729889.1"
                     /translation="MNILIIGNGGREHALAWKVRQSPLADKVFVAPGNAGTAMENGIE
                     NVAISATDVPALVTFAKQNSVGLTIVGPEAPLVVGVVDAFRAHGLTIFGPTQAAAQLE
                     GSKAFTKDFLARHQIPTAEYQNFTEIEPALAYLKQKGAPIVIKADGLAAGKGVIVAMT
                     LQEAEDAVRDMLSGNAFGEAGSRVVIEEFLDGEEASFIVMVDGKNVEPMATSQDHKRV
                     GEGDSGLNTGGMGAYSPAPVVTKAIHQRVMDEIIYPTVQGMASENTPYTGFLYAGLMI
                     MPNGQPKVIEFNCRFGDPETQPIMMRLESDLVELCLKACEGKLDSVQSKWCEQAALGI
                     VLAAAGYPGDYRKGDEIIGIPTSTSNQKVFLAGVEQKEGKLLTNGGRVLCATALGNSV
                     FEAQQQALQLAEQIQWQGKFYRRDIGYRAVARKQAK"
     gene            1837..2211
                     /gene="rpsF"
                     /locus_tag="PWX02_001905"
     CDS             1837..2211
                     /gene="rpsF"
                     /locus_tag="PWX02_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDL1729890.1"
                     /translation="MRHYEIVFMVHPDQSEQVPAMIERYTASVKEAGGQVHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQSVIDELETNFRYNDAVLRNLIVHTKAAVTEASPM
                     VKAKESKVAEAVAEVESEEAGE"
     gene            2186..2560
                     /gene="priB"
                     /locus_tag="PWX02_001906"
     CDS             2186..2560
                     /gene="priB"
                     /locus_tag="PWX02_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HDL1729891.1"
                     /translation="MNQRKQASNLPIDNCLILSGTVSSLVKQSLNPLGVPHYSFWLEH
                     RSIQTEVNLERQAWCKIQVVLAGNQFSYLTQQIKLGESVRIKGFIHTHKDFNGLNQLV
                     LHTEQIEFIDQEKPNGTLFPSS"
     gene            2535..2762
                     /gene="rpsR"
                     /locus_tag="PWX02_001907"
     CDS             2535..2762
                     /gene="rpsR"
                     /locus_tag="PWX02_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDL1729892.1"
                     /translation="MARYFRRRKFCRFTAENVVEIDYKDIATLKNYISESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLALLPYTDNHQ"
     gene            2778..3227
                     /gene="rplI"
                     /locus_tag="PWX02_001908"
     CDS             2778..3227
                     /gene="rplI"
                     /locus_tag="PWX02_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDL1729893.1"
                     /translation="MQVILLDKVAHLGSVGDQVTVKSGFARNFLIPQGKAVMATAANI
                     AHFEARRAELEAKAAAALATAQERAAKIAALAAVSVSATAGDDGRLFGSVSAKDIAEA
                     LTAAGVGVAKSEVRLGEGPLRTTGEHQVKIHLHPEVNATVAVNVVAE"
     gene            complement(3291..4067)
                     /gene="dnaQ"
                     /locus_tag="PWX02_001909"
     CDS             complement(3291..4067)
                     /gene="dnaQ"
                     /locus_tag="PWX02_001909"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HDL1729894.1"
                     /translation="MTEEKIIRQVVLDTETTGMNMAGGPPQIGHNIIEIGAVEVINRR
                     LTGRTYHVYIKPPREVDEEAIAVHGITNEFLQDKPVFAEIAEEFLDFIKGAELIIHNA
                     PFDVAFMDQEFSYLPNPPAKTAEMCTVTDSLQMARRMYPGKRNNLDALCDRLGIDNSK
                     RVLHGALLDAEILADVFLMMTGGQISLLGEEETEVVSEVIDIEEEFSAIVLNADEAII
                     LKANDEEEESHLALLKLIEKKSKGNCLWTKALTDTAEISH"
     gene            complement(4155..5825)
                     /gene="ettA"
                     /locus_tag="PWX02_001910"
     CDS             complement(4155..5825)
                     /gene="ettA"
                     /locus_tag="PWX02_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HDL1729895.1"
                     /translation="MSTQFVYTMHRVGKVVPPKRHILKDISLSFFPGAKIGVLGLNGA
                     GKSTLLRIMAGVDKEFEGEARPQPGIKIGYLPQEPKLAPQQTVREAVEEAVAEVKNAL
                     TQLDEVYAMYADENADFDKLAAKQAELEAIIQAHDGHNLQNQLERAADALRLPDWDAK
                     IEHLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDYEGTVVAITH
                     DRYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKEKRLAQEQAAESARQKSIEKELEW
                     VRQNPKGRQAKSKARMARFEELNSGEYQKRNETNELFIPPGPRLGDKVIEVQNLTKSY
                     GDRTLIDDLSFAIPKGAIVGIIGANGAGKSTLFRMLSGKEQPDSGSIVLGETVVMASV
                     DQFRDAMDDKKTVWEEVSNGQDILTIGNFEIPSRAYVGRFNFKGVDQQKRVGELSGGE
                     RGRLHLAKLLQRGGNVLLLDEPTNDLDVETLRALENALLEFPGCAMVISHDRWFLDRI
                     ATHILDYGDEGKVTFYEGNFSDYEEWKKKTFGEAATQPHRVKYKRIAK"
     gene            6031..>6477
                     /locus_tag="PWX02_001911"
     CDS             6031..>6477
                     /locus_tag="PWX02_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729896.1"
                     /translation="MYVQKHSNYSTALLVVIREKINSLPHQGLILDLISISKDDLDDI
                     LEGNTDMNIHQLLSLIYKAGFFDVLNITNNLCIKLSQFGFYFQNGNLSQKDDELLSLY
                     REFYKHNEFITHLKSHSLYGCIEQPTSAFFHYCTNENFRNAFNEESL"
BASE COUNT         1731 a         1456 c         1342 g         1948 t
ORIGIN      
        1 ttaaatcctc ttcaaatttt ctgtattctt tataagcgtt aattaaattt ttctcaatag
       61 aaattggaga tggattcttc acaagaactt tatctccctt accttgattt tcttttctat
      121 ttaaagatct ttttagtctt tgccaatatt gacagttttt tccacatata gggcacgcag
      181 agtcatttag aaaagagttt actgcatcgt ttaatgatag tgatttaatt tcggggcctg
      241 catcgagttc agtgatatta atttcattaa gtgttgtact cataataaat ttttccttgt
      301 tttaagagtt tataaattat ttcgcttgtt ttcttgccac tgctcggtag ccaatatctc
      361 ggcgatagaa tttgccttgc cattgaattt gttcggcaag ttgtaaggct tgttgttgag
      421 cttcaaatac actgttgcca agtgcggtgg cacaaagtac acgtccaccg ttggtgagca
      481 gttttccttc tttttgctca acccctgcta agaaaacttt ttgattggag gtagaggtcg
      541 gaatgcctat gatttcatcg cctttacggt aatcgcccgg gtagcctgcg gcagctaaaa
      601 caatgcctaa tgcggcttgt tcacaccatt ttgattgcac gctatccaat ttgccttcgc
      661 acgcttttaa gcacaattca acgaggtctg attccaaacg catcataatc ggctgagttt
      721 cagggtctcc aaaacggcag ttaaattcaa tcactttcgg ctgaccgttt ggcataatca
      781 ttaagccggc atagagaaaa ccggtgtaag gggtattttc gcttgccatt ccttgcacgg
      841 tcgggtaaat aatttcgtcc attacacgtt ggtggattgc ctttgttacc accggagcag
      901 gggagtaagc ccccattccg ccggtattta agccgctatc gccctcgcca acacgtttgt
      961 ggtcttgtga ggttgccatt ggttcgacat ttttgccgtc caccatcaca ataaaactgg
     1021 cctcttctcc gtctaaaaat tcctcgatca ccacacggct gccggcttca ccaaaggcgt
     1081 tgcctgacag catatcacgc actgcatctt ccgcctcttg taaagtcatt gcaacaatta
     1141 cgcctttgcc tgcggctaaa ccgtctgctt tgattacaat cggtgcacct ttttgtttta
     1201 agtaggcaag tgccggttca atttcggtga agttttggta ttctgcggtt ggaatctgat
     1261 ggcgagccaa gaaatctttg gtaaatgctt tagaaccttc taattgagca gcggcttggg
     1321 tcgggccgaa aatggttaaa ccgtgggctc gaaaagcatc aaccacgcca actactaacg
     1381 gtgcttcagg gccgacaatc gtcaaaccta cgctattttg tttagcgaat gtaactaagg
     1441 caggcacgtc agtggcggaa atagctacat tttcaatgcc gttttccatc gctgtgccgg
     1501 cattgccggg ggcgacaaag actttgtctg cgagcggcga ttgacgaact ttccaagcaa
     1561 gagcgtgttc acgcccaccg ttgccgataa ttaaaatatt cattgggatt ccttgcaaaa
     1621 atgataaagc aaacgtttgc gtattgtact tgatttgttt atctgctgac aagcggtcgt
     1681 ttttctcgga ttttttgcaa aatacgaaat tgttgcttgc aaaataggct tttttcagta
     1741 taatcctgcc gttcagtcac attcgttttt taatccttgc ttccacaagt tggtgactgg
     1801 ctaaagttgg aggctgctaa cccgtaagga tagtcaatgc gtcactacga aatcgttttt
     1861 atggttcacc cggaccaaag cgaacaagta ccagcaatga ttgagcgcta cacagcgtct
     1921 gtaaaagaag ctggcggtca agttcatcgc ttagaagatt ggggtcgtcg tcaattagca
     1981 tacccaatca acaaacttca caaagcacac tatgtgttaa tgaatgtaga agcacctcaa
     2041 agtgtaatcg acgagttaga aactaacttc cgttataacg atgcagttct tcgtaactta
     2101 atcgttcaca ctaaagcggc agtaactgaa gcgtcaccaa tggtgaaagc gaaagaaagc
     2161 aaagttgctg aagcggtagc tgaagttgaa tcagaggaag caggcgagta atttgccgat
     2221 tgataattgt ttaatcttaa gcggaacggt ttccagcttg gttaaacaaa gcctaaaccc
     2281 ccttggtgtg cctcattaca gtttttggtt agaacatcgt tcaatccaaa ccgaagtgaa
     2341 tttagaacgc caagcgtggt gcaaaatcca agtggttttg gcaggcaatc aatttagtta
     2401 tttaacccaa caaattaagc tcggtgagag cgtgagaatt aaaggtttta tccatactca
     2461 caaagacttt aacggtttaa atcagttagt cttacacacc gaacaaattg aatttataga
     2521 tcaggagaag ccaaatggca cgttatttcc gtcgtcgtaa gttctgccgt ttcacagcgg
     2581 aaaatgttgt tgaaatcgat tacaaagata tcgctacatt aaagaactac atttctgaaa
     2641 gcggcaagat tgttcctagc cgtattactg gtactcgtgc gaagtatcaa cgtcaattag
     2701 ctcgtgcaat taaacgcgct cgctaccttg cgttacttcc atatactgat aaccaccaat
     2761 aatcaggaga gagtacaatg caagttattt tattagacaa agttgctcac ttaggttctg
     2821 tgggcgacca agtaaccgtt aaatctggtt ttgctcgtaa cttcttaatc ccacaaggta
     2881 aagcggttat ggcaactgcg gcaaacattg ctcactttga agcacgtcgt gcagaattag
     2941 aagcaaaagc tgcagcagca ttagcaactg cacaagaacg tgcagcgaaa attgcagcat
     3001 tagcagcagt atcagtatct gcaactgcag gtgatgatgg tcgtttattc ggttctgtat
     3061 cagctaaaga catcgctgaa gcattaactg cagcaggtgt tggagttgct aaatctgaag
     3121 ttcgtttagg tgaaggtcca cttcgtacaa ccggcgaaca ccaagttaaa attcacttac
     3181 accctgaagt gaatgcaact gtagcagtaa acgttgttgc tgaataatca gtagaaatag
     3241 aaaacccagc gaaagctggg ttttttgttt gctttattta ttggttagaa tcagtgagat
     3301 atttctgctg tgtcggttaa tgcttttgtc cataggcaat ttcctttgga ttttttctca
     3361 atcagtttca gaagtgctaa atgcgactct tcctcttcat cgtttgcttt aagaataatt
     3421 gcctcatcgg catttaacac aattgcagaa aattcttctt caatatcaat aacttcactt
     3481 accacttcag tttcttcttc gcctaataat gaaatctgac cgccggtcat cattaggaat
     3541 acatcagcta agatctccgc atccagtaac gccccgtgta gtacacgctt gctattatca
     3601 atgccgagcc tgtcgcaaag tgcatctaaa ttattccgtt tgccggggta cattcttcgt
     3661 gccatttgca ggctatcggt tacggtacac atttctgcgg tttttgccgg tggattaggc
     3721 agataagaaa attcttgatc cataaacgcc acatcgaagg gggcattatg aataattaat
     3781 tccgcacctt tgatgaaatc caaaaactcc tcggcaattt cagcaaaaac aggtttatct
     3841 tgcaaaaatt cattggtgat gccgtgaact gcaatcgcct cttcatctac ctcacgcggt
     3901 ggcttgatat aaacgtgata agttctgccg gttaaacggc ggttaatgac ttcaaccgca
     3961 ccgatctcaa taatattatg cccaatctgt ggcgggccac ctgccatatt cattcctgtg
     4021 gtttcagtat ccagcactac ttggcggata attttttctt ctgtcatatt caatttaaaa
     4081 ttgtaggggt gaaatatttt tatcccttaa ttctgcattt gattaggggc gaaacatatt
     4141 tcgcccctac attgttactt cgcaatccgt ttatatttca ctcggtgcgg ttgggtggcg
     4201 gcttcaccga aggttttctt cttccactct tcatagtcgg agaagttacc ctcgtagaac
     4261 gtcactttgc cttcatcgcc gtagtctaaa atatgggtgg cgatacggtc taagaaccaa
     4321 cggtcgtgcg aaattaccat tgcacagccc gggaactcta agagcgcatt ttccaacgca
     4381 cgcagggttt ctacgtctaa gtcgttggtc ggctcgtcca gtaacaacac gttaccgcca
     4441 cgttgtagta gcttcgccaa gtgtaaacga ccacgttcac cgcctgaaag ctcgcccaca
     4501 cgtttttgct gatctacgcc tttgaagttg aaacgcccca cataagcacg gcttgggatt
     4561 tcaaagttac caatcgtgag aatatcctgc ccgttcgaca cctcttccca cacggttttc
     4621 ttatcgtcca tcgcatcacg gaactgatcg accgatgcca ttaccaccgt ttcaccaagc
     4681 acgattgaac cagaatccgg ctgttcttta ccagaaagca tacggaacaa ggttgattta
     4741 cccgcaccat ttgccccgat aatcccgaca atcgccccct tcggaatagc gaacgacaag
     4801 tcgtcaatca aagtgcgatc gccgtaagat ttagtcaggt tttgtacctc gatcacttta
     4861 tcacctaagc gtggacccgg tggaataaag agttcgttag tctcgttacg tttttgatat
     4921 tcgcctgagt taagctcttc aaagcgtgcc atacgagcct tacttttcgc ttggcgacct
     4981 tttggatttt ggcgtaccca ttccaactct ttctcaatcg atttttgacg agccgattcc
     5041 gccgcttgct cttgtgccaa gcgtttttct ttctgctcca accaagaaga gtagttacct
     5101 tcccaaggaa tgccttcacc acggtcaagc tctaagatcc agcccgcaac gttgtctaag
     5161 aagtaacggt cgtgggtaat tgccactacg gtgccttcgt agtcgtggag gaaacgctcc
     5221 aaccacgcca ccgattccgc atctaagtgg ttggtcggct catctaacaa taacatatcc
     5281 ggtttttcca gcaacagacg gcaaagtgcc acacggcggc gttcaccccc tgataaatgc
     5341 tcgatttttg cgtcccaatc cggcagacgt aacgcatcgg cagcacgctc taactggttt
     5401 tgaaggttat ggccatcgtg ggcttgaata atcgcctcta attccgcctg ttttgccgct
     5461 aatttgtcga aatcggcatt ctcatcagca tacatcgcat agacttcatc aagctgggtt
     5521 aaggcgtttt ttacttccgc aaccgcttct tccaccgctt cacgcacggt ttgctgtggg
     5581 gcaagtttcg gttcttgtgg aagatagccg attttaattc ccggttgtgg acgagcctcg
     5641 ccctcgaact ctttatctac gcccgccata atgcgtaaaa gcgttgattt acccgccccg
     5701 tttaagccga gcaccccgat tttcgccccc gggaaaaagc tcagggaaat atctttaaga
     5761 atgtgacgct tcggcggcac gactttgccg acacggtgca tcgtatatac aaattgagtt
     5821 gacattggtt gtattcctca caaaaaaatt cgccttattt tacttgaatt tacagccaaa
     5881 atatagggtg gttttggctt ttggtaaagt aaccttttcc aatagagatg ttatttgagg
     5941 ctgcatagta ctatgcattt ctcttacctt gacaggtaag agaaacaaat cctgaaggaa
     6001 aaaaatttta aggaagttat tctatgcaat atgtatgtcc aaaaacactc aaattattca
     6061 acagcattgt tggttgttat aagggagaag attaattctc tacctcatca aggcttgatt
     6121 ttagatttaa tatccatttc taaagatgat ttagatgata ttttggaggg taatactgat
     6181 atgaatattc atcaattatt aagcctgatt tataaggctg gattttttga tgtattaaac
     6241 atcacaaata atctatgtat aaaactcagt caatttggtt tttatttcca aaatggcaat
     6301 ttgagccaaa aagatgatga gcttttgagt ttatatagag aattctataa acataacgag
     6361 tttatcactc atctcaagtc ccatagtctt tatggatgta ttgagcaacc tacttcggca
     6421 ttttttcatt attgtactaa tgaaaacttt agaaatgcgt ttaatgaaga gagcctc
//
LOCUS       DANJWC010000132         6446 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.132, whole genome shotgun sequence.
ACCESSION   DANJWC010000132 DANJWC010000000
VERSION     DANJWC010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6446
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.132"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            43..732
                     /locus_tag="PWX02_001912"
     CDS             43..732
                     /locus_tag="PWX02_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL1729897.1"
                     /translation="MSTLSRDSILSQIQNGLIASCQPVDDGPMDKPEIVAAMAAASVI
                     GGAKGIRIEGVDNLKAARAAVNVPIIGIVKRDLPDSPVRITPFLEDIDALAEAGADII
                     AVDGTFRTRPVSIEEAVKRIHEKGCLAMADCSTLEEGLHCQQLGFDIVGSTMSGYTGG
                     EVPDEPDYQLVKDLKAAGCNVMAEGRYNTPELAKTAIEIGADYVTVGSALTRLEHIVS
                     WFADSVKSAQK"
     gene            742..1644
                     /locus_tag="PWX02_001913"
     CDS             742..1644
                     /locus_tag="PWX02_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HDL1729898.1"
                     /translation="MRCLAIDIGGTKIATAIVQHNQVSQRQQIQTPTDKPQADQAGSL
                     HQAIGEIMQHYQGQFDFVAVASTGIINKGVLTALNPKNLGGLAEFPLKQSIEKHTDKP
                     IALLNDVQAAVCAEYLNEDQQAVKNFVFITVSTGVGGGIILNGELQIGSNGIAGHIGH
                     TLADPNGPLCGCGRVGCVEAVAAGRAIEAVSSQWEKPCSPKEVFERFRQGNPQAVELV
                     QKSAKAIANLIADLKISLDIQKVVLGGSVGLAEGYLPQVSNYLAEMPSVYHCILENAL
                     SGQDAGLIGAAWWAENQLNQGFTL"
     gene            1669..2547
                     /gene="nanA"
                     /locus_tag="PWX02_001914"
     CDS             1669..2547
                     /gene="nanA"
                     /locus_tag="PWX02_001914"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005718701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDL1729899.1"
                     /translation="MKNLKGIFSALLVSFNEDGTINEKGLRQIIRHNIDKMKVDGLYV
                     GGSTGENFMLSTEEKKEIFRIAKDEAKDEIALIAQVGSVNLKEAVELGKYATELGYDS
                     LSAVTPFYYKFSFPEIKNFYETIIRETGNKMIVYSIPFLTGVNIGVSQFAELFENEKI
                     IGVKFTAGDFYLLERLRKAFPNHLIYAGFDEMMLPATVLGVDGVIGSTFNVNGIRARQ
                     IFELAQQGKIKEAFEIQNVTNDLIEGILGNGLYQTIKGLLELEGVQAGYCREPMTKEQ
                     TPAQKEVTKQLKAKFL"
     gene            complement(2608..2813)
                     /locus_tag="PWX02_001915"
                     /pseudo
     CDS             complement(2608..2813)
                     /locus_tag="PWX02_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253447.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2771..3416)
                     /locus_tag="PWX02_001916"
                     /pseudo
     CDS             complement(2771..3416)
                     /locus_tag="PWX02_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3521..5116)
                     /locus_tag="PWX02_001917"
     CDS             complement(3521..5116)
                     /locus_tag="PWX02_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1729900.1"
                     /translation="MRLSQLSLSVLLLSASSLVAAAPVNTLINCVATPPMKLSPAITN
                     DANDFNASSQQIYNRLLAFKAGELTVEPSLAERWEISDDGLSYTFHLRKDVKFHSNKQ
                     FTPTRTLNADDVVFSFQRQMDLNHPYHKVSDGTYFYYHWMNLPKILKTVEKVDDYTVK
                     ITLQQPNAPFLTTLAMDFLSIYSKEYADKLLAEGKPELLDQAPIGTGPFVFQAYQTDQ
                     AVRYVANPDYFQGKAKFEKLIFSITPDAGTRYAKLKAGECDVIDFPNISDIAQMKQDP
                     KVNLLQRDGLNLAYIGLNSTKPALNNAKVRQALHYATDKKAIVEAVYQGGGTVASNPF
                     PDSVLGYNPNLPQYEFNLEKAKALLTEAGFADGFETEIWVQPVVRPSNPNPRRTAEII
                     QADWAKIGVKAKLVTHEWADFNKRTREGEFSTGTYGWTSRNGDPDNFLFPLLSKSNIP
                     GTNYGRWTDEKFEQLLAQAVQTQNRDERAKLYQQAVEIANEQTPFIPLAHAINYVPLS
                     KRVQGFVQNPFGYTPFYGVSLSE"
     gene            5265..5705
                     /gene="mioC"
                     /locus_tag="PWX02_001918"
     CDS             5265..5705
                     /gene="mioC"
                     /locus_tag="PWX02_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HDL1729901.1"
                     /translation="MTSICIITGSTLGGAEYVADHINEVLSEQGFEVELFNQATLADI
                     EGKANLIVVTSTHGAGELPENIQPLFDDLANSTADFSSMKFGVIGLGSSDYDTFCNAV
                     NIVEEALKAKGASQVCESLRIDVMNNFDHDGSAEEWLPSFIEQL"
     gene            5717..5884
                     /locus_tag="PWX02_001919"
     CDS             5717..5884
                     /locus_tag="PWX02_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HDL1729902.1"
                     /translation="MKKISANKAKWIKIAIILIYMFSPVDILPEAILGPLGLVDDAAA
                     LMLLIKTILEK"
     gene            complement(5973..6446)
                     /gene="asnC"
                     /locus_tag="PWX02_001920"
     CDS             complement(5973..6446)
                     /gene="asnC"
                     /locus_tag="PWX02_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HDL1729903.1"
                     /translation="MYSKNTTKSEIDKLDQKILRWLIKDARTPYAEMAKVFGVSAATI
                     HVRVEKMRQAGIIESTKIVVDERKVGYDVCCFIGIILKSAKDYDKVIAKLSEFNEVVE
                     AYYTTGNYSIFIKVMTHTIEELHSVLAGKIQSIDDIQSTETLISLQNPISRDILP"
BASE COUNT         1926 a         1343 c         1348 g         1829 t
ORIGIN      
        1 ctagtccatt gactaccttt tgaacataaa aaaggaactg ttatgtcaac actatctcgt
       61 gattctattt taagtcaaat tcaaaacggg ctgattgcct cctgccaacc ggttgatgac
      121 ggccccatgg ataaacctga aattgtcgcc gcaatggcag cagcctctgt gattggtggt
      181 gctaaaggca ttcgcattga aggtgtcgat aatttaaaag ccgctcgtgc ggcagtgaat
      241 gtgccgatta tcggcattgt aaaacgtgat ttgccagatt cccctgtgcg tattacgcca
      301 tttttagaag atattgatgc cttagccgaa gccggtgccg atattattgc ggtggacggc
      361 acattccgca cccgcccggt cagcattgaa gaggcggtaa aacgcattca cgaaaaaggt
      421 tgcttggcaa tggcggattg ctccaccctt gaagaaggtt tacattgcca acaattaggc
      481 tttgatattg tgggtagtac aatgtctggc tacaccggtg gcgaagtgcc tgatgagccg
      541 gactatcaat tagtgaaaga tttaaaagct gccggctgta atgttatggc ggaaggtcgc
      601 tacaacacgc ctgaactggc aaaaacggcg attgaaatcg gggcggatta cgttaccgtt
      661 ggatcggcat tgacccgctt agagcatatt gtgagctggt ttgccgactc tgtaaaatcg
      721 gctcaaaaat aggagctgct tatgcgttgt ttagcaatag acatcggtgg cacgaaaatt
      781 gccacggcaa ttgtgcaaca caatcaagtg tcacaacgcc aacaaattca gaccccgact
      841 gataaaccac aagctgatca ggcaggcagt ttgcatcaag cgattggtga gataatgcaa
      901 cattatcaag ggcaatttga ttttgtggca gttgcctcaa caggcattat caataaaggg
      961 gtgcttaccg cattaaatcc taaaaattta ggcggattgg cagaatttcc gttgaaacaa
     1021 agcattgaaa aacataccga taagccgatt gcattattaa atgacgtgca agcggcggtg
     1081 tgtgccgaat atttaaatga agaccaacaa gcggtcaaaa attttgtatt tattaccgtt
     1141 tccaccggcg taggcggcgg cattatttta aacggcgaac tgcaaatcgg cagtaacggc
     1201 attgcaggac atatcgggca tactctagcc gatccgaacg ggcctctctg tggttgtgga
     1261 cgagtcggct gtgtcgaagc agtcgcagca gggcgtgcaa tagaggcagt ttccagccaa
     1321 tgggaaaagc cttgttcacc gaaagaggtg ttcgagcgat tccgccaagg caacccacaa
     1381 gcggtcgaat tagtacaaaa atctgcaaaa gcgattgcta atttaattgc agatttaaaa
     1441 atcagtttag atatacaaaa agtggtgtta ggtggcagtg tgggcttagc ggaagggtat
     1501 ttaccgcagg tgtccaacta tttagctgaa atgccgagcg tatatcattg catattggaa
     1561 aatgctctat ccgggcaaga tgccggctta atcggtgcgg cttggtgggc ggaaaaccaa
     1621 cttaatcaag gttttactct ttaacaaatt ctaactccgg agatccaaat gaaaaactta
     1681 aaaggtattt ttagtgcgtt actcgtctct ttcaacgaag acggcaccat caacgaaaaa
     1741 ggcttacgcc aaattatccg ccacaacatc gacaaaatga aagtggacgg tttatatgtc
     1801 ggcggttcaa ccggtgaaaa ctttatgctt tctaccgaag agaaaaaaga aattttccgc
     1861 attgccaaag acgaagcgaa agatgaaatc gcccttatcg cccaagtcgg tagcgtaaac
     1921 ttaaaagagg cggtagaact gggtaaatat gccacagaat taggctacga cagcttatct
     1981 gcggtcacac cgttctacta caaattcagc ttcccggaaa tcaaaaattt ctacgaaacc
     2041 attattcgtg aaaccggcaa caaaatgatt gtttactcca ttccgttctt aaccggcgta
     2101 aacatcggtg tgagccaatt cgctgaatta tttgaaaacg aaaaaatcat cggtgtaaaa
     2161 ttcactgccg gcgatttcta cctgttagag cgtttacgca aagcgttccc gaaccactta
     2221 atctacgcag gtttcgatga aatgatgcta ccggcaacgg tattaggtgt ggacggtgta
     2281 atcggctcaa ccttcaacgt aaacggtatt cgtgcaagac aaatctttga attagcccaa
     2341 caaggcaaaa tcaaagaagc atttgaaatc caaaatgtaa ccaacgattt aatcgaaggc
     2401 attttaggca acggtttata ccaaaccatc aaaggcttat tggagttaga aggtgtgcaa
     2461 gcaggttact gccgtgagcc gatgacgaaa gaacaaactc cggcacaaaa agaagtgacc
     2521 aaacagttaa aagctaaatt cttgtaattt aagttgcaaa aggctaaacg aaaatcaccg
     2581 cttgtagtaa caagcggtga ttttttctca acgacttgca attaatgaag ccattctgct
     2641 ttttctacca cggtgtaacg gtcaccatta tattttaatc gcactttttc ttggcgagta
     2701 ttttctgatt tcaactctct acctatatca taaaaccctg tactccagat attttgagta
     2761 atatccgctt ttacaatgat atcattgaag ccgttggtct gtttgttatc catttgcaat
     2821 accaagtttg tcgtttccgt tttggtttct ttatcatcta aaatagacca aaaagtcatc
     2881 gggtaatcaa aaacaagttt taacttttct ttttccggca caaataacat caaatgatca
     2941 tttgcagaat actcaggagc acttggacca cgagcagcat tattaaacct cacgccgaaa
     3001 gctcttactt tgtcgtttag aatataacgg gcggtatcaa ttttaagact atctgcattc
     3061 acatcgatag aggcatcgat ctccatatcc tgctcccacg attgcaatat cgcattatca
     3121 ttcacacgag caacaaaaag cgtccactta ggtgtgtttt cgtcgttttg ataattttct
     3181 ttgctgtatt taggctctat taccgcataa ctcactaagg tttccgttga attgttagga
     3241 gctgttttac aggcagaagc taaagtacga gcagaaagtt tctcttgaga aatcttaaag
     3301 taatgagcaa taatttgatg ttgttgctca gcacaaacct gtgtgttatt accttcatct
     3361 gcttttattt gagttgcaag cgtaagtaaa agaactaaga ggagtatctt tttcatatag
     3421 atttatccgt atagaaagat aactcattct aaaagaaaag cagtctattc tctaccacat
     3481 tttgcaaaaa gtggcaaaaa tagaccgctt gtatggcata tcactccgat aaactcaccc
     3541 cataaaatgg cgtgtagcca aacggatttt gcacaaaacc ttgcacccgt ttgctgagtg
     3601 gtacataatt gatagcgtga gccagcggaa taaatggcgt ttgttcgttg gcaatttcca
     3661 ccgcttgttg atagagcttc gcccgctcgt cacggttttg ggtttgtaca gcttgagcta
     3721 ataactgctc aaatttctca tccgtccaac gcccataatt tgttcccgga atattagatt
     3781 tgcttaataa agggaagagg aaattatcag gatcaccatt acggcttgtc caaccataag
     3841 tgccggtaga gaactcgcct tcacgggtgc gtttgttaaa atccgcccat tcgtgggtca
     3901 ctaatttcgc tttcaccccg attttcgccc aatccgcctg aataatttca gccgttcgac
     3961 gtgggtttgg gttagaaggg cgcactaccg gttgtaccca aatttccgtt tcaaatccat
     4021 cggcaaagcc ggcttctgtt aatagggctt tggctttttc aaggttaaat tcatattgcg
     4081 gtaaatttgg attatagccc aacaccgaat cagggaacgg attacttgcc accgtgccac
     4141 cgccttgata aacagcctcg acaatcgctt tcttatcggt tgcataatgc aaggcttggc
     4201 gaactttggc gttatttaat gccggtttgg tgctgtttaa gccgatataa gctaaattca
     4261 gcccgtctct ctgtaacaaa ttcaccttcg gatcttgttt catttgggca atatcggaaa
     4321 tattcgggaa atcaatcaca tcgcattcac ccgctttcag tttagcataa cgagtgcccg
     4381 catcaggggt aatactaaaa atcagtttct caaattttgc tttaccttgg aaataatccg
     4441 ggttcgcaac ataacgcacc gcttgatcgg tttgataggc ttggaacaca aaaggccctg
     4501 tgccgatcgg ggcttgatcc aataactccg gcttgccttc cgccagcaat ttatccgcat
     4561 attcttttga ataaatcgac aagaaatcca tcgctaaggt tgtcagaaaa ggagcattag
     4621 gttgctgtaa ggtgatttta acagtgtaat cgtccacttt ttccactgtt tttaaaatct
     4681 tcggcaaatt catccaatgg taatagaaat aagtaccgtc agagacctta tggtaagggt
     4741 ggtttaaatc catctggcgt tggaacgaga acactacatc atccgcattc agcgttcgag
     4801 ttggcgtaaa ttgtttattg gaatgaaatt tcacgtcttt acgtaaatgg aatgtgtaac
     4861 ttaagccatc gtcactgatt tcccaacgtt ccgccaaact cggctcaacc gttagttcac
     4921 cggctttaaa cgctaataag cgattataaa tttgttgtga actggcgttg aaatcattgg
     4981 catcattagt aattgcagga ctaagtttca tcggtggagt tgccacgcaa ttaatcaacg
     5041 tatttaccgg agctgctgcc accaaactgc tggcacttaa gagtaaaaca gaaagagata
     5101 attgagaaag acgcataaag actccttaac attttctact aacttactcg gcattaacgt
     5161 attttcaaaa taactaaagg ttataattta taatcagaat aatcagttgc aaaccaatga
     5221 ttcttggcta gaatattggc agttttttaa acgagagatt tactatgaca tcaatttgta
     5281 tcatcaccgg cagcacctta ggcggtgcgg aatatgttgc cgatcacatt aatgaggtat
     5341 taagcgagca aggttttgaa gtggaactgt ttaaccaagc cacccttgcc gatattgaag
     5401 gtaaagccaa tctgattgtc gttacctcca cccacggagc cggcgagcta cccgaaaata
     5461 tccagccgtt atttgatgat ttagcaaata gcacggcgga tttcagctca atgaaatttg
     5521 gcgtaatcgg cttaggcagc tcagattacg acaccttctg caatgcagta aatattgttg
     5581 aagaagctct caaagcgaaa ggggcaagcc aagtttgcga atcactgaga attgatgtaa
     5641 tgaataattt cgaccacgat ggcagtgctg aagaatggtt accatcattt attgaacaat
     5701 tataacttaa gtatcaatga aaaaaatcag tgcaaataag gcgaagtgga ttaaaattgc
     5761 tattatctta atctatatgt tcagccctgt agatattcta cccgaagcaa ttttaggccc
     5821 actcggtttg gttgatgatg ctgccgcatt aatgctattg attaagacta ttttggaaaa
     5881 ataggcatag tagacaaaat agttgcaaaa aaccaaaaca aaacgaccgc ttgtaaccca
     5941 atacaagtgg tcttatcttt tacttaattc ttttaaggga gaatatctct tgaaatagga
     6001 ttttgaagtg aaataagcgt ttctgttgac tgaatatcat caatagactg aatttttcct
     6061 gctaataccg aatgcaactc ttcgatggta tgggtcatta ctttaataaa aatagaataa
     6121 ttaccagtgg tatagtacgc ctcaacaact tcattaaatt cactaagttt agcaattact
     6181 ttatcataat ctttggctga ttttaaaata atgccaataa agcaacacac gtcataccca
     6241 accttacgct cgtccactac gattttggta gattcaataa tgccggcttg gcgcattttt
     6301 tcaacacgga catggattgt tgcggcactc acaccaaaaa cttttgccat ttccgcataa
     6361 ggcgtgcgag catctttaat tagccaacgt aaaatttttt gatccagctt atcaatttcc
     6421 gatttagtcg tgtttttact gtacat
//
LOCUS       DANJWC010000133         6397 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.133, whole genome shotgun sequence.
ACCESSION   DANJWC010000133 DANJWC010000000
VERSION     DANJWC010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6397
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.133"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..588
                     /locus_tag="PWX02_001921"
     CDS             <1..588
                     /locus_tag="PWX02_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HDL1729904.1"
                     /translation="IHFSHLRVINEDFIAPNHGFGKHPHDNMEILTYMLKGRVAHEDS
                     MGNKTEVKAGEFQIMSAGTGVFHSEINPDPNETLHLYQIWIIPNQKDVTPRYNQDRFA
                     PKEGATLILSPNAEEGSFKIYQDMKLWRYQYPTAKSEQLALEPNRSYWLQVVKGNLNI
                     NGVELSTSDALGIRQEKALEINTQDDVEFLLFDLV"
     gene            complement(656..1447)
                     /locus_tag="PWX02_001922"
     CDS             complement(656..1447)
                     /locus_tag="PWX02_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDL1729905.1"
                     /translation="MNNLRIKMISPEIALAKYNELDVLEINHSKVKAKIALQGAQLLS
                     WQPKNTQQDVLWLSEIEPFQTGVAIRGGIPICHPWFGTDKKPAHGTARIQAWNLLEHT
                     SSADGIRVVLGLENQAKLEMILGEVCELYFTHLAPEPAQAALHTYFQVGDITKTEVQG
                     LATQCFDKLTNQEIEVPSPRKISENVDCIYPAQAVNFIQDFANQRTIKVEHINATETV
                     LWNPWHNTVSAMTETAYQNMVCVETARINTLLQQNETLGVRILVR"
     gene            1571..2575
                     /gene="fbp"
                     /locus_tag="PWX02_001923"
     CDS             1571..2575
                     /gene="fbp"
                     /locus_tag="PWX02_001923"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HDL1729906.1"
                     /translation="MKTLGEFIIEKQAEYPGAKGELSGILSSIRLAAKIIHREINRAG
                     LSQDILGTAGSENVQGEAQMKLDVFANETMKKALLAREDVAGFASEEDDNFVAFDNER
                     GRNAKYILMTDPLDGSSNIEVNVSVGTIFSIYRRVSPIGTPVTQEDFLQEGRKQVAAG
                     YVTYGSSTMLVYTTGNGVNGFTYDPSLGLFILSHPDLKTPTEGKYYSINEGQYVTFPQ
                     GVKRFIKYCQETDEAIKRPYSSRYIGSLVSDFHRNLLKGGIYIYPTSTVYPKGKLRLL
                     YECNPIAFLAEQAGGMATDGINPILDIKPTELHQRVPFFVGSTSMVKQAGEFMQEFAK
                     "
     gene            2759..4864
                     /locus_tag="PWX02_001924"
                     /pseudo
     CDS             2759..4864
                     /locus_tag="PWX02_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250422.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S6"
     gene            complement(5074..5802)
                     /locus_tag="PWX02_001925"
     CDS             complement(5074..5802)
                     /locus_tag="PWX02_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1729907.1"
                     /translation="MKSFDKVWFSAKELEELDLPDLPKQATNITRKALKELWIKRDAV
                     GVKGGGNEYHITSLPRKAQEALGFKSGINAIKDSIVREITPEEQNKGSELSGTVLNNI
                     ISMIDSFASIKVSAGFGSFNECVTKPDGQEPYADSLLHTLGVNAKKCAVFWASGDSMY
                     PTIDNGDQLLVDLSRNEIRGDNNIYIVQNGDSVWVKRVKLLWDGVELISDNREEYELI
                     KITADQAQNLQIIGQVVHIGHSLV"
     gene            6012..6239
                     /locus_tag="PWX02_001926"
     CDS             6012..6239
                     /locus_tag="PWX02_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1729908.1"
                     /translation="MNKKKRAQDMHRADIRAELMKKGITFSQLGIENGLSKTTVRNAL
                     DKPYKNGEEIIAKALGKTPAEIWPSRYPDVA"
     gene            6254..>6397
                     /locus_tag="PWX02_001927"
     CDS             6254..>6397
                     /locus_tag="PWX02_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDL1729909.1"
                     /translation="MKDWFSAKELAGISGLSKYPTNVTRQAKKEQWATRPLKGVKGGG
                     FEYH"
BASE COUNT         2028 a         1301 c         1239 g         1829 t
ORIGIN      
        1 atccattttt cacatttacg ggtaattaac gaagatttca ttgcacctaa tcacggtttt
       61 ggcaagcacc cgcacgataa tatggaaatt ttaacttata tgttaaaagg acgtgtggct
      121 cacgaagaca gtatgggtaa taaaaccgaa gtaaaagctg gcgaatttca aattatgtct
      181 gccggaaccg gggttttcca ttcagaaatt aatccagatc cgaatgaaac cttacacctt
      241 taccagattt ggattatccc aaaccaaaaa gatgtgaccc ctcgctacaa ccaagataga
      301 tttgccccga aagagggagc aaccttgatt ctttcgccaa acgcagaaga aggttcattt
      361 aaaatttacc aagatatgaa gctgtggcgt tatcaatacc caacagccaa atcagagcaa
      421 ctggcattag agccaaaccg cagttactgg ctacaagtgg tgaaagggaa tctaaacatc
      481 aatggcgttg aactttccac cagcgatgcc ttaggcattc gtcaagagaa agcacttgaa
      541 ataaacactc aagatgacgt ggaattttta ctgtttgatc tagtgtaaat acaagagaca
      601 agcggttaaa tttgtaaaaa atattactaa ttcagctgct tgctctcatt aaacattatc
      661 gcaccaaaat tctcactcct agcgtttcat tttgttgaag taaggtattt atccgtgctg
      721 tttccacaca taccatattt tgatacgccg tttctgtcat cgcactaacg gtattatgcc
      781 aaggattcca aagtacggtt tcggtagcat tgatatgctc aactttaatg gtgcgctgat
      841 ttgcaaaatc ttgaataaaa ttcaccgctt gtgccggata aatacaatcc acattttcac
      901 ttatcttgcg aggtgatggc acttcaattt cttgatttgt caatttatca aaacattgtg
      961 ttgctaagcc ttgtacttcc gttttcgtaa tatcaccgac ctgaaaataa gtatgcaatg
     1021 cagcctgtgc cggctccggt gctaagtgag taaaatacaa ctcacagact tcgcctagta
     1081 tcatctcgag ttttgcctga ttttctaacc ccaaaacaac acgaatccca tctgctgagg
     1141 aagtatgttc cagcaaattc cacgcttgaa tacgggctgt gccgtgagca ggctttttgt
     1201 ctgtaccaaa ccaagggtgg caaatcggaa tgccgccacg aatcgccaca cctgtttgga
     1261 aaggttcaat ttcacttaac cataatacat cttgctgagt atttttaggt tgccaactca
     1321 gtaactgcgc accttgcaat gcaattttag ccttcacttt ggagtgatta atctctaaaa
     1381 catccaattc attatatttt gctaatgcta tttcagggct gatcattttg attcgtagat
     1441 tattcatcgt attgctcctc atcttgaaag ataaaatgtg aagtggatca cagaattaaa
     1501 gcttaaaccg ctacctcttt aaaggtaatc gttataataa gggcattcta acatagcgaa
     1561 ggagaaaaaa atgaaaacac tcggcgaatt tattattgaa aaacaagcag aatacccagg
     1621 ggcaaaaggg gaattaagtg ggattttatc ttctattcgt ttagccgcta aaattattca
     1681 tcgagaaatc aaccgtgccg gtttaagtca agatatttta ggtactgcag gttctgaaaa
     1741 tgtacagggt gaagcccaaa tgaaacttga tgtattcgcc aacgaaacga tgaaaaaagc
     1801 gttacttgca cgtgaagatg ttgccggctt tgcatcggaa gaagatgata attttgttgc
     1861 ctttgataat gagcgtggga gaaatgcaaa atatatttta atgaccgatc cgcttgatgg
     1921 ctcctccaat attgaagtga atgtttctgt gggaacaatc ttctctattt accgccgtgt
     1981 ctcccctatc ggcaccccag taacacaaga agatttcttg caggaagggc gtaaacaagt
     2041 tgccgccggt tatgttactt atggttcttc aaccatgctg gtttacacca caggcaatgg
     2101 cgtgaacggt tttacctatg atccgtcatt aggcttattt attctttcac accctgatct
     2161 aaaaacccct accgaaggaa aatattactc catcaatgaa gggcagtatg ttactttccc
     2221 acaaggtgtg aaacgcttta ttaagtattg ccaagaaacc gatgaagcca tcaaacgtcc
     2281 atactcttca cgctatatcg gctcgctggt gtccgatttc caccgcaatt tattaaaagg
     2341 cggtatttat atttacccaa cctccaccgt atatccaaaa gggaaattgc gtttactcta
     2401 tgaatgcaac ccgattgcgt ttcttgccga acaagccgga ggaatggcga cagatggtat
     2461 taatccgatt ttagatatta agccaacaga gctacaccag cgtgtaccgt tctttgtcgg
     2521 ttcaacctca atggtcaaac aagcgggcga atttatgcaa gaatttgcaa aataatgctc
     2581 ctgcttatca ctcaataaaa ctttcaacaa agcagctgcg tattccgtag ctgcttttgc
     2641 ctatcttaag attgtaaatt tgtataaaaa acaggtaaga cctcttgcca aataaataat
     2701 tatctataga ataccccacc tttttactcc attaatatta gcaatttatt aacattttat
     2761 gataaataaa aactttccac tttcttacct tgccagcttt gtggcatttg ctgttagttt
     2821 atctgcaaca gcctctgttt tcagagatga tatcgatctt caatactacc gtgattttgc
     2881 ggagaataag ggtaaattca ccgtaggtgc aagcaatatt gaaattaaag ataaaagtgg
     2941 caatacatta gatgcactct tcccaaatgt accgatgcca gattttagtg ctgcaaaccg
     3001 taacttaggg atagcaactc tagttgcacc gcaatattta gtcagcgttg cccataatac
     3061 acaatacaat actgtggaat ttggcgcccc aggcactaat gccgatgcac accactacac
     3121 ttacaaagtc gttgatcgta acgactatgg aattgttgaa ggtgggcaac atcaagatta
     3181 tcaagtgcca cgtctcaata aattagtcac tgaggtcgca cctgcaacag tgactgatct
     3241 gggtaataat gcttcagcct accaagatag tagtcgtttt actcactttg ctcgcttagg
     3301 ctctggtcgt caaatagtca aaaataccga gcataaagac aaccaaattt caacatctta
     3361 ccaatattta accgggggag ttcatttacc aatcgcggta cataattccg attactggct
     3421 agattttaga ggaaatgcat taaacaatag cccatatgga gcattaactg catttggtac
     3481 tcgtggtgat agcggttcag gtgtgtatgg ctacgatcag aaaacgaaac gctggctact
     3541 actggctact tacacctttg gtacgcctgc aaacaactac tacaaccgtg ccggtattat
     3601 ccgtcaggat tatcacgata aacaatttgc cgaagatatt gccggcacat taaccaatgc
     3661 gcaacaaaat gccgtttttg aatggtctgc acagggtaaa gacagtagca ttggtaataa
     3721 agggaaaaac ttaacggttt cgcttgctga tcgttcaatt aaagacaata gcgcttctcc
     3781 attaggctat ggtggcacac ctcagctaca acttccgcaa gataacaccg gaaaaaccct
     3841 aaacattgaa ggacaagata gcactatcgt attaaaaacc gatattgacc aaggtgctgg
     3901 cgcattaaat ttcaatgcta atgcaacagt tcgccctgaa aatgatcaaa gctgggtagg
     3961 agctggaatt gtagtggcaa aaggtaaaca agttaattgg caagtcaaaa acccacaagg
     4021 tgaccgttta tctaaattgg gcaaagggac actacatatt aacggtaaag gcgaaaacct
     4081 tggcgacatt agtgttggac aaggcactgt cattttgaac caacaagcgg gtgaaaatgg
     4141 caaaaaatct gcatttaacc aagttggtat tgtgagcggc cgttcaaccg ttgtattaaa
     4201 cagtgctgat caagtcgatc caaataagat ttactttggc ttccgtggtg gtcgtttaga
     4261 tctcaacggc aacaacattg cgtttaatcg tattcaaaat agtgatgatg gtgcgcgtat
     4321 tgttaataat cacctacaaa aagctgcaac actcaccatt aatggcccga agccccctga
     4381 agcaacggac ttaaaatggg gaacgtggaa agaaaacagt gctgatattt atgaatatat
     4441 caacccacat gctaataacc gtaccgatta ctttacgctc aaaggcaatc caaaccaata
     4501 tatgccaacg aatggcgcaa gtaatgccca ttggaccttc ctgtcttcta acaaggatgc
     4561 agcagtaaaa caagtactcg cgcaaaaagg tctggagcat cgctacaata gctttaatgg
     4621 ttttattggc gaaacagata acacccagca taacggtcgt ttgaatgtgg tgtatgatcc
     4681 aaaagcctct acaccaccgg caactgcctc tgaagtaact tggggtaagg ggctagtagc
     4741 tggtgcagat atttatctgt tcaccaatcc gaaaacgaaa attcgggaat actttgcatt
     4801 aaaaggtgat ccaaaacaac ctgttcctcg cggtggctta agcagcgagc attgggaatt
     4861 tttagtgtgg ggtactgatc ttttttgcat agagagaaag aaaaaaattt gggcggaaaa
     4921 ataacggcga gccccgtgaa tacaagggtt cgccgttatt gtttggttgg agctatgcaa
     4981 aaaacaaaat gagcaggatt agaaaaaaac gagcagtttt tgcgttattt tttgcatagg
     5041 tttgatcggt tttaaaacta ttttaaatct gttttaaact aaagaatgcc cgatgtgtac
     5101 cacttgcccg atgatttgta ggttttgggc ttggtctgct gtaatcttta ttaactcgta
     5161 ctcttctcgg ttgtctgaaa taagctctac accgtcccat agcagtttta cacgcttaac
     5221 ccaaacactg tcgccgtttt gtacaatgta aatattattg tcgcctcgta tctcattacg
     5281 acttaaatca actagtagct ggtcgccatt atcaatagtc ggatacatcg agtctccact
     5341 tgcccaaaaa acagcacact tcttggcatt aacaccaagc gtatgcaaca agctatcagc
     5401 ataaggctct tgtccatcag gttttgttac acattcatta aagctcccaa agcctgcaga
     5461 tactttaata gatgcaaaac tatcaatcat tgagattatg ttattcaaaa cggtgcctga
     5521 taattcagat cctttgtttt gctcttcagg tgtaatctct cttactatgc tatccttgat
     5581 tgcatttatt ccacttttaa atccgagtgc ttcttgggct ttacgaggca atgaagttat
     5641 gtgatattca ttccccccac cttttactcc aactgcatca cgttttatcc agagttcttt
     5701 taatgccttt cttgtaatgt ttgttgcctg ctttggaagg tcaggcaaat ccaattcttc
     5761 tagttctttg gctgaaaacc aaactttatc aaatgatttc ataattaacc ccttcttaat
     5821 caaaaatgat tttaaaatca aaattgatta agttatttaa aatcaatagc ttacaaatag
     5881 atttaaaaaa gatttaaaaa atttattaaa tcactattga tttagaaaag aataagtatt
     5941 atatttagtc ctgtagttaa tcaagtacgc taaagcgtag caaataaact aaatgtggag
     6001 gatctcacaa aatgaataaa aagaaaagag ctcaagatat gcatagagct gatattcgag
     6061 ctgaattgat gaagaaagga ataacatttt ctcagttagg gattgagaat gggctttcca
     6121 aaacaacagt aagaaatgct cttgataaac cttataaaaa cggtgaagaa attatcgcaa
     6181 aagcattagg aaaaacgcca gccgaaatat ggcctagtcg ttaccctgat gttgcataac
     6241 aaggatagct gttatgaaag attggtttag tgcaaaagaa ttagcaggta ttagtggctt
     6301 atcaaaatac ccaacaaatg taactagaca agccaaaaaa gaacaatggg caactcgtcc
     6361 attgaaaggg gttaaaggag gtggttttga atatcac
//
LOCUS       DANJWC010000134         6377 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.134, whole genome shotgun sequence.
ACCESSION   DANJWC010000134 DANJWC010000000
VERSION     DANJWC010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6377
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.134"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            151..1473
                     /gene="pepP"
                     /locus_tag="PWX02_001928"
     CDS             151..1473
                     /gene="pepP"
                     /locus_tag="PWX02_001928"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HDL1729910.1"
                     /translation="MDLAYLAKMPQEEFTARRERVLEQMQDNSALLVFTESEKRRNSD
                     SEYLFRPDSYFWYLTGLAEPKSAVLLVKSEGKTESIIFVRKKDPLMETWNGRRLGIEE
                     APKTLQLNAAFDIDDIETVLAKKLENLTACYCALGIQEWGDNILFATFEKMKVNRQKV
                     PTTLIDWQPMLSEMRLIKSEFEIALIQQACHISSMAHIRAMKQARPNRYEWEIEGEIQ
                     HEFSRFGARFPAYNSIVAGGENACILHYNENDQVLKGGDLLLIDAGAEFAYYAGDITR
                     TFPINGKFSEPQKELYELTLTMQKEAINLLVPNSSIKAANDKAVQILTQGLVRLGILN
                     GDVESLIEQKAYRQFYMHGLGHWLGLDVHDVGDYGTERDRPLQIGMVLTVEPGIYIPK
                     DADVPEQYKGIGIRIEDNLLITEYGNKNLSSGCPKEIADVEQLMQSLN"
     gene            1589..1774
                     /locus_tag="PWX02_001929"
     CDS             1589..1774
                     /locus_tag="PWX02_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HDL1729911.1"
                     /translation="MYFEIYKDAKEEFRWRLKAGNHEVIATSQAYTTKQGCQKGIEAV
                     KKVTPETEVKDLTKAEA"
     gene            1903..3219
                     /gene="argA"
                     /locus_tag="PWX02_001930"
     CDS             1903..3219
                     /gene="argA"
                     /locus_tag="PWX02_001930"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HDL1729912.1"
                     /translation="MQNTELALAQWFRQSTPYVNMHRGKTFVIMLDGDVIESPNFVNI
                     INEISLLHSLNIKLVIVFGARPQINQLLKQNHIEPEYYHNIRVTNPQSLEYVKQAVGK
                     VHYDFFARLSLRLPHSEVINVVSGNMILAQPIGVVDGVDYALSGKIRRINIESIKQQL
                     AQNSIVLLGPIAPSVTGEMFNLPFEDIATQVAIKLRADKLIGFCEEQGILDENGKPIP
                     DYLPQDGETLLQQLIERGEYHTPKARFLQAAITVCREGVKRSHLLSYKEDGSLLQELF
                     SRDGIGTQFSMESSEQVRIANSSDIPGLLDLIRPLEQQGILVKRSREQLEMEITNYTI
                     IERDGVVIACAALNPYPEEKMAEMACVAVHPDYRDSSRGDILLENIQKRAVQLGMEKL
                     FVLTTRTTQWFQERGFRLADAQDLPRYKRENYNYQRRSKVLILPLW"
     gene            3273..4160
                     /locus_tag="PWX02_001931"
     CDS             3273..4160
                     /locus_tag="PWX02_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012621753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL1729913.1"
                     /translation="MKKHQPIKGAMAIITAGVLFACINTLIPKLTSISPIDSSVIALV
                     QYLVAFIFLVPSMNNIGFFSSLKTEHFGQHCFRIFLSAIGIQCWTMALANQLPIWQGI
                     ALLMTSPLFVTIGSGLFLKEKVDKKRWIATAMGFVGAMIILEPWSDYFEMIALLPIAA
                     AFFWAGYSLMVKKLSRYDSPTTMVAYLFILITPFNLLIALTNLSPSGFSLPSWSDFGF
                     LILLGFFTALAQLAVAKAYSVADASYIQPFDFIKLPLNVLAGWLVFNWVPPGKLWLGA
                     AIIIAATIYITHAETKQSN"
     gene            complement(4240..4731)
                     /gene="ilvN"
                     /locus_tag="PWX02_001932"
     CDS             complement(4240..4731)
                     /gene="ilvN"
                     /locus_tag="PWX02_001932"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDL1729914.1"
                     /translation="MRRTLAVLLENESGALSRVVALFSQRGFNIESLTVAPTDDPSLS
                     RMTIVAQGEEQVLEQIEKQLHKLIDVFKVSNLSAYEHVEREVLLVKVRATGSSRDELK
                     RMVDIYRGQIVDLTPKLYTIQLSGSTEKLNAFIEAVKEETTIVEIVRSGVISLSRGDK
                     NAL"
     gene            complement(4724..>6377)
                     /locus_tag="PWX02_001933"
     CDS             complement(4724..>6377)
                     /locus_tag="PWX02_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HDL1729915.1"
                     /translation="GYPGGSVLDIYDAIHTLGLNHVLVRHEQGAVHMADGYARSTGKV
                     GCVLVTSGPGATNTITGILTAYTDSVPLVILSGQVPSSLIGTDAFQESDMIGISRPVV
                     KHSFLVKNTEEIPEIIKKAFYIASTGRPGPVVIDIPKDVVNPLNKYPYEYPQEVSMRS
                     YNPTVQGHKGQIKKALKALLVAKRPVLYVGGGVISAECSPEITEFAQKLNLPVTCSLM
                     GLGAYPASDKQYLGMLGMHGTFEANNAMHESDLILGIGVRFDDRTTNNLAKYCPNAKV
                     IHVDIDPASISKTVQAYIPIVGSAKNVMEEFLSLLGEENLAKNQVDLTAWWQQIDAWK
                     ARNCLAFEENSDVIKPQQVIRMIHKLTNGEAYVASDVGQHQMFAALHYGFDKPRRWIN
                     SGGAGTMGFGLPAAMGVKFAHPDATVVCVTGDGSIQMNIQELSTAKQYDTPIVIVSLN
                     NRFLGMVKQWQDIIYSGRHSQVYMNSLPNFAKLAEAYGHVGIQIDHPSELEAKLTEAF
                     SIKDKLVFVDVLVDETEHVYPMQVRGGAMNEMMLSKTERTDA"
BASE COUNT         1889 a         1237 c         1329 g         1922 t
ORIGIN      
        1 gtaaaggtta tatttaatct taatctgttt tgtttttgca atcaagatct caaatataat
       61 ctttcatctg ttttggttag gctgaattta tgctgcgttt gtgctaaaat aaggcaggtt
      121 tcgatataga tataataaaa aggagaaata atggatctcg cttatttggc taaaatgcca
      181 caagaagaat ttaccgcacg ccgtgagcgt gtgcttgagc aaatgcagga taattctgcg
      241 ttattggttt ttaccgaatc ggaaaaacgc cgtaatagtg actctgaata cttgtttaga
      301 cctgatagtt acttttggta tttaaccggt ttggcagaac ctaaatcagc cgtattgctg
      361 gtaaaaagcg aaggtaaaac ggaaagtatt atttttgtgc gtaaaaaaga ccctttaatg
      421 gaaacctgga atggcagacg tttagggatt gaggaagctc caaaaacgtt acagcttaac
      481 gcagcttttg atattgatga cattgaaacc gttcttgcaa aaaaattaga aaatctgacc
      541 gcttgttatt gtgcgttagg gatacaagag tggggcgata atattttgtt tgcaacattt
      601 gaaaaaatga aagtcaatcg gcaaaaagtg ccgacaacct tgattgattg gcagccaatg
      661 ttatcggaaa tgcggttgat taaatcagaa tttgaaattg ccttaattca gcaggcgtgt
      721 catatttctt caatggcaca tattcgagcg atgaaacaag ctcgcccgaa tcgttatgag
      781 tgggaaattg agggggaaat tcaacacgaa tttagccgtt ttggagcaag gtttccggca
      841 tataattcaa tcgttgccgg cggtgagaat gcctgtattc ttcactacaa tgaaaatgat
      901 caggtgctta aaggcggcga tttattactg attgatgcag gggcggaatt tgcgtattac
      961 gccggcgata ttacccgaac cttccctatt aatgggaaat tctccgaacc gcaaaaagag
     1021 ctgtacgaat taaccttaac tatgcaaaaa gaggcgatta atcttcttgt gccaaacagc
     1081 tcgattaaag cggcaaacga taaggctgta cagattctca cgcagggctt ggttcgctta
     1141 ggaattttaa acggtgatgt ggaaagcctg attgagcaaa aagcctatcg ccaattttat
     1201 atgcacggat tagggcattg gctagggcta gatgttcacg atgtggggga ttacggcacg
     1261 gagcgggatc gtcctttgca gattggtatg gtgttaactg ttgaacccgg tatctatatt
     1321 cctaaagatg cggatgtacc ggagcaatat aaaggtattg ggattcggat tgaagataat
     1381 ttgctgatta ccgaatacgg caacaaaaac ttaagcagcg gttgccctaa agaaattgcg
     1441 gatgttgagc agttgatgca atcgttaaat taattcgcaa atttttgtaa aaaaatgacc
     1501 gcttgctttg atattttggg gaatttagct taagctaacc cgacacaaac acagtgtttg
     1561 ggcaatcaca acacgaagga gtgtttatat gtattttgaa atttacaaag atgcaaaaga
     1621 agaatttcgt tggcgtttaa aagcgggtaa ccacgaagtt attgcaacca gccaagccta
     1681 tacgactaag caaggttgtc aaaaaggcat tgaagcagtt aaaaaagtta cacctgaaac
     1741 agaagtaaaa gatttaacca aagctgaagc ataatctata aagccctgat attatttcag
     1801 ggctttgtta tttatgcaaa tataatgctt aattattcaa tttaaggctt gcggattttt
     1861 ctaaaaaaga taatatgcgt ttatattata aagaggaaat atatgcaaaa cacagaactc
     1921 gcattagctc aatggtttag gcaatctaca ccttatgtta atatgcacag aggcaaaacc
     1981 tttgtgatta tgcttgatgg cgatgttatc gaaagcccga attttgtcaa tattatcaat
     2041 gaaatcagtc tcctgcacag cctgaatatc aaattagtga ttgtatttgg ggcaagaccg
     2101 caaattaacc aattgttaaa acaaaatcat attgagccgg agtattacca caatatccgt
     2161 gtgactaatc cacaatcgct agaatatgtg aaacaagcgg tagggaaagt tcattatgat
     2221 ttctttgccc gcctttcttt gcgtttaccc cattctgaag tgattaatgt agtcagtggt
     2281 aatatgattt tagctcagcc gattggcgtg gtggatgggg tagattatgc actaagcggt
     2341 aaaatccgtc gtattaatat tgagagcatt aagcagcaac tggcacaaaa ttcgattgta
     2401 ttacttggtc cgattgcccc gtctgttacc ggtgaaatgt ttaatctgcc gtttgaagat
     2461 attgccacac aggttgcaat taaacttcgt gccgataaat taatcggttt ttgtgaagag
     2521 caggggattt tagatgaaaa cggcaagcca attccagatt atcttccgca agatggcgaa
     2581 accttgttac agcagttaat cgagcgtggc gaatatcaca ccccgaaagc tcggtttttg
     2641 caggcggcga tcacggtatg ccgagaggga gtgaaacgct cgcatttatt aagctataaa
     2701 gaagatggtt cattgcttca agagttattc tctcgtgatg gtattggcac acagttctca
     2761 atggaaagct cggaacaggt acgtattgct aattccagtg atattccggg attattggat
     2821 ttaatccgcc cacttgaaca gcagggcatt ttagtcaaac gctctcgaga acaattagaa
     2881 atggaaatca caaactacac cattattgag cgtgatggtg tggtgattgc ttgtgcggca
     2941 cttaacccgt atccggaaga aaaaatggcg gaaatggcgt gtgtggcggt tcaccctgat
     3001 tatcgtgatt cctcaagagg ggatattcta ctggaaaata ttcaaaaacg ggcagtgcaa
     3061 ctgggaatgg agaaactctt tgtgctgact acaagaacca cacaatggtt ccaagagcga
     3121 ggatttaggc tggcagatgc ccaagattta cctcgctata aacgggaaaa ttacaattat
     3181 caacgtcgtt ctaaagtatt aattttacca ctttggtaaa atattcaatg tttttgaccg
     3241 cttgttacca agagaactat aaaaggagag ctatgaaaaa acatcaaccc atcaaagggg
     3301 ctatggcaat tattaccgca ggtgtattat ttgcctgtat caatacctta attcccaaat
     3361 taacgtccat ttcaccaatt gattcgagtg tgattgcact ggtgcaatat ttagtcgcct
     3421 ttattttcct cgtacccagt atgaataata tcggtttctt ctcttcatta aaaaccgagc
     3481 attttgggca acactgtttt cggatttttc tatctgcgat tggtattcaa tgttggacaa
     3541 tggcactcgc taaccaatta cctatttggc agggcatagc gttattgatg acttcgccat
     3601 tgtttgtgac catcggctcc ggtctgtttt taaaagagaa agtggataaa aagcgttgga
     3661 ttgcaactgc aatggggttt gttggggcaa tgattattct agagccttgg agcgattatt
     3721 ttgaaatgat tgcactgttg ccgattgctg ctgctttttt ctgggcaggt tattcgctaa
     3781 tggtgaaaaa actctctcgc tacgatagcc caactacgat ggtcgcttat ctgtttattc
     3841 taattacgcc ttttaatttg ttgattgcac tcacgaacct tagccctagt gggtttagtc
     3901 tgccttcttg gagcgatttc ggctttttaa tcttactcgg tttctttacg gcgttagcac
     3961 aactggcggt agccaaagcc tacagtgtag cagatgcttc ctacattcag ccgtttgatt
     4021 ttattaaatt gccgctgaat gtattagccg gttggcttgt atttaactgg gtgccaccgg
     4081 gtaaattatg gctaggggca gcgattatta tcgctgcgac catttatatt actcacgctg
     4141 aaactaaaca gagtaattag caacaaattc acgttacata aaaaatccct ttatctttaa
     4201 gataaaggga tttgcaaatt ttaagctaaa attgaccgct tacagagcgt tcttatcacc
     4261 acgagatagg ctaatcacgc cggagcgaac aatctctaca atcgttgttt cttctttaac
     4321 tgcctcaata aatgcattga gcttctcggt agaaccggat aattgaatgg tgtaaagttt
     4381 cggtgttaaa tctacgattt gcccacggta aatatctacc atacgtttca gttcatcacg
     4441 agatgaacct gtagctcgca cttttaccaa taacacttca cgctcaacgt gttcataagc
     4501 acttaagtta ctcactttaa acacatcaat gagtttgtgt agttgctttt cgatttgttc
     4561 taacacctgt tcttcgcctt gcgcgacaat tgtcatacgg gaaaggcttg gatcatcagt
     4621 aggggcaacg gtcaagcttt caatgttaaa accacgctgc gagaaaaggg caactacacg
     4681 agataatgca cctgattcat tttctaataa tacggctaat gttctacgca tctgttctct
     4741 ccgttttact taacatcatt tcattcattg ctcctccacg cacttgcatt gggtaaacat
     4801 gttccgtttc atcgaccaat acatcaacaa atacaagctt atctttaatt gagaatgcct
     4861 cagttaattt tgcctccaat tcagaagggt ggtcgatttg aatcccaacg tggccataag
     4921 cctctgctaa tttagcaaag ttaggtagag aattcatata cacctgtgaa tggcggccag
     4981 agtaaataat atcttgccac tgtttcacca tacctaagaa acggttattt aagctgacaa
     5041 tcacaatcgg tgtgtcgtat tgctttgcgg tggaaagttc ctggatattc atttggatac
     5101 taccatcacc ggttacacaa acaacagtgg catcagggtg ggcaaatttt acccccatcg
     5161 cagcaggtaa gccaaagccc attgtgcctg caccgcctga attaatccaa cgacgtggtt
     5221 tatcgaaccc ataatgcaat gcagcaaaca tttgatgttg tccaacatct gaggcaacat
     5281 aagcctcccc attggtcaac ttgtggatca ttctaatcac ttgttgtggc ttaatcacat
     5341 cactgttttc ttcaaaggct aagcaattac gagctttcca agcatcaatt tgttgccacc
     5401 aagcggttaa atctacctga ttttttgcaa gattttcctc acctaataaa gataaaaatt
     5461 cttccattac attcttagca ctgcctacaa ttggaatata agcttgtact gttttggaaa
     5521 ttgaggctgg atcaatatcc acgtggatca cttttgcatt cgggcaatat tttgctaagt
     5581 tatttgttgt acgatcatca aaacgtaccc caatacctaa aatcaaatca ctttcgtgca
     5641 ttgcattatt tgcctcaaaa gtgccgtgca tgcctaacat acctaaatat tgcttatcag
     5701 aggcaggata agcacctaat cccattaatg aacaggttac aggtagattt agcttttgtg
     5761 caaactctgt aatttctggc gaacactctg cactaattac accaccgcct acataaagca
     5821 ctggacgttt tgctactaat agtgctttta atgctttttt aatttgccct ttatgccctt
     5881 gaactgttgg gttataagaa cgcattgata cttcttgcgg atattcatag ggatatttat
     5941 ttaatggatt gactacatct ttaggaatat caataactac cgggcctggt cgaccggttg
     6001 atgcaatata aaaggctttt ttgataattt caggaatttc ttctgtattt tttactaaga
     6061 aactgtgttt tacaaccgga cgagaaatgc cgatcatatc actttcttgg aatgcatctg
     6121 tcccaattaa acttgatgga acttgacccg acagtattac taacggaaca gagtcggtat
     6181 aagcagttaa aataccagta attgtattcg ttgctcccgg acctgaagtc actagcacac
     6241 agccgacttt acctgttgaa cgggcatagc catcagccat atgtacagca ccttgctcgt
     6301 gtcgaactaa aacgtgattc aatccaagtg tatgaattgc atcataaata tcgagtacag
     6361 agccacccgg atagccg
//
LOCUS       DANJWC010000135         6324 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.135, whole genome shotgun sequence.
ACCESSION   DANJWC010000135 DANJWC010000000
VERSION     DANJWC010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6324
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.135"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            77..439
                     /locus_tag="PWX02_001934"
     CDS             77..439
                     /locus_tag="PWX02_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729916.1"
                     /translation="MYQVFIAEPKDPNLEKGLLKHFIKSYLTQEELDFLHKILTNLPN
                     IRPFWGDTGYKYIVIDNVYFLYEVSESEKKVILLGTKFNNKRKTFNFANTKTQKKSMK
                     KWINDLAIYKPHKINQSQ"
     gene            452..1369
                     /gene="nagK"
                     /locus_tag="PWX02_001935"
     CDS             452..1369
                     /gene="nagK"
                     /locus_tag="PWX02_001935"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005708433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDL1729917.1"
                     /translation="MAIYYGLDVGGTKIELAAFNEKLEKLHSERVPTPQTNYQDWLTA
                     IKTLVETADTKFDEKGTVGLGIPGFVNRETGIAEITNIRVADGKTILKDLAECLGREV
                     RAENDANCFALSEAWDESNKPYSTVLGLIIGTGFGGGFVFDGKIHSGRIGMAGEVGHM
                     QLNYHALKLLDWDKAPIYACGCGNQACLDTYISGRGFELLFNDLVGEKISAKAIIERF
                     YQQETKVVEFVEKYIELMAISISNLITILDPDMIVFGGGLSNFDYIYQALPKALPKHL
                     MRSAKVPVIKKAIHGDSGGTRGAAALFLK"
     gene            complement(1440..3386)
                     /locus_tag="PWX02_001936"
     CDS             complement(1440..3386)
                     /locus_tag="PWX02_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL1729918.1"
                     /translation="MYQRSQSRTVLFPILLFVGINLVILTLSRLVLGVWQSDRVSAVD
                     GWIPLILQGIRIDISSLCWLFGLPALFSVLCFHKNVLGSIWQTVLRIWLTVGSVFILF
                     MEVATPNFIETFDLRPNRLFIEYLVHPREVFTMLINGHLWAVICGIGIAMISLIIYWK
                     LSGWATKNLHFPSWKMRPLLALLVAAIVFVGGRSSFAHRGINPAMVAFSSDPLVNSLV
                     LNSGYSVLYAVQQLKDEDKSSEQYGKMSVEEMFNIVKQVRNRPASDYISDEIPTLTQN
                     KATYQGKPKNIVIILEESLGAQFVGSLGGKNLTPNLDKLSEQGWYFTNLYATGTRSVR
                     GIEAVTAGFTPTPARSVVKLTKSQTNFFSIAELLKRQGYHTSFIYGGEKHFDNMASFF
                     YGNGFQQIIDEKDYKNPKFTATWGVSDEDLFDKANGTFNQLHKSGKPFFSLVFSSSNH
                     DPFEFPDGKIELYEQPKQTRHNAAKYADYAIGHFFELAKKSEYWQDTVFLVIADHDSR
                     AVGEHLVPIQHFHIPALLLGEHIQPRTDNRLVSQLDMPTTLLSVAGISGQYPMIGYDL
                     TQDDPNRAFMQYDATQAMMRGNDVVVMRPNLPIEGFSYDKANYKLTPKEQPESFKKEA
                     LAHALLGSYLYKNQLYKLPEETKQ"
     gene            complement(3418..4206)
                     /locus_tag="PWX02_001937"
     CDS             complement(3418..4206)
                     /locus_tag="PWX02_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HDL1729919.1"
                     /translation="MQVTELNIYPIKSTQAIQVSQAFVEPKGLNFDREFMLAEPNGKF
                     ITARKDPELYRLAAFPIATGVMITHTSGQKCVALYQDFVEEQSSEVWGTHFNAKVAAE
                     AVNQWLSEIFGRAVQLRWLGSQSQRIVANFADHPMSFADSNPVSLMSLKSLEQVQQWS
                     PIPLEMARFRPNIVIDGNVAFEEEQWQQVQIGEVLFTKAALCTRCILITRDLNTLELD
                     PKSEPFRTLKEKHCDEKGKPVFGIHLVPQNSGVIKVGDPVIIQL"
     gene            4331..4561
                     /gene="xseB"
                     /locus_tag="PWX02_001938"
     CDS             4331..4561
                     /gene="xseB"
                     /locus_tag="PWX02_001938"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDL1729920.1"
                     /translation="MAKKPTQDFESTLKELEAIVTHLESGDLPLEDALNEFETAIKLV
                     QQGQERLQKAEQRIQILLNKNDTAPLSDYERE"
     gene            4567..5460
                     /gene="ispA"
                     /locus_tag="PWX02_001939"
     CDS             4567..5460
                     /gene="ispA"
                     /locus_tag="PWX02_001939"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HDL1729921.1"
                     /translation="MSYLLATDLTHLQQRINHFLAEKLKTTDSVGSPLVEAMAYAVLL
                     GGKRVRPFLIYATGRMLGVPLEKLDHSAAAMEAIHAYSLVHDDLPAMDDDRLRRGKPT
                     CHIAFDEATAILAGDALQSFAFELLATDPHLTDREKVAQVTELATASGAKGMCLGQSL
                     DLIAENKAVSLEELELIHRNKTGALILSSVMMGLNLSEHSQNLAIKQPLVNYAKAIGL
                     AFQVQDDILDVIGDTDKIGKTVGSDEHLNKSTYPKLLGLDGAKQKAEMLYQTALHSLE
                     QLPFDTTALKELANFIVKREN"
     gene            complement(5494..6294)
                     /locus_tag="PWX02_001940"
     CDS             complement(5494..6294)
                     /locus_tag="PWX02_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HDL1729922.1"
                     /translation="MCQLLGMNCNTPTDITFSFDGFRRRAGLTDHHTDGFGIAFFEGK
                     GVRVFRDNRPGASSPMAEAISQYQIKSLNVVAHIRKATRGDVNLENTHPFIRELWGEN
                     WVFAHNGTVEGVGVCEECHYQPIGTTDSEAAFCRIVSELKEMYPAKPSEKEIFDAVVQ
                     ITSRIAEHGVFNFVLSNGHWMIARCTTNLHYVCRKAPFGKALRDDDVSIDFSQYTKIT
                     DKVTIITTQPLTKNENWTKMKNGGYVFFKDGDVVYEIEGTLPEAKPHV"
BASE COUNT         1951 a         1343 c         1255 g         1775 t
ORIGIN      
        1 caggctagtg ctgatacttg ggaggcaatc ttaaataaca ctattcaacc ttttgacttc
       61 aaaaaatagg cttgctatgt atcaagtttt tattgcagaa ccaaaagacc caaacttaga
      121 gaaaggctta ttgaaacact tcattaagtc ttatcttact caagaagaac tcgatttcct
      181 gcataaaatt ctcactaatt tacccaacat cagacctttt tggggcgata ccggttacaa
      241 atatatcgtc attgataatg tttacttctt gtatgaagtt tccgaaagtg aaaagaaagt
      301 cattctactt ggcaccaaat ttaataataa acggaaaact tttaattttg cgaatacgaa
      361 aacccagaaa aaatcaatga agaaatggat taatgattta gcaatttata aaccgcataa
      421 aatcaatcaa tcgcaataag agagcgtaat tatggcaatt tattatggct tagatgtggg
      481 cggcacaaaa attgaattag ccgcttttaa tgaaaaacta gaaaaactac acagcgaacg
      541 tgtgccgaca ccgcagacaa attatcaaga ttggctgacg gcaattaaaa cgctggttga
      601 gactgccgat acaaaatttg acgaaaaagg cactgtcggg ctgggcattc cgggctttgt
      661 aaaccgtgaa accggcattg cggaaattac caatattcga gtggctgacg gcaaaacgat
      721 tctgaaagat ttagccgaat gtttagggcg tgaagtgcgt gccgaaaacg atgctaactg
      781 ctttgccctg tctgaagcgt gggacgaaag taataagcct tattccaccg tattagggtt
      841 gattatcggc accggttttg gcggtggttt tgtgtttgac ggtaaaattc attcaggcag
      901 aatcggaatg gcaggcgaag ttgggcatat gcaacttaac taccacgcac ttaaattgct
      961 cgactgggat aaagccccaa tttatgcatg tggttgtggc aaccaagcct gtttagacac
     1021 ctatatttca gggcggggtt ttgagttact gtttaatgat ttagtaggag aaaaaatctc
     1081 ggcgaaagcg attatcgaac gcttctacca acaagaaacc aaagtggtgg aatttgtcga
     1141 aaaatatatt gagctaatgg caatcagtat cagcaatctg attacgattt tagatccgga
     1201 tatgattgta ttcggtggcg gtttatccaa ctttgattat atctaccaag ctctaccaaa
     1261 agcattacca aaacacttaa tgcgttcagc gaaagtgccg gtcatcaaaa aagcaattca
     1321 cggcgactca ggtggcactc gtggtgcagc agcattattc ttaaagtaat gaacaaattt
     1381 gagagagggc aagcggtcat ttttaataaa aatattgcag ccctctcttt taatttgcct
     1441 tactgcttag tttcttccgg taatttatat aactgatttt tatataaata actgccaagc
     1501 aatgcgtgtg ctaacgcctc ttttttaaac gattccggct gttctttagg ggtgagttta
     1561 tagttagctt tatcgtagct aaatccttct atcggtaaat ttggtcgcat taccaccaca
     1621 tcatttcccc gcatcatcgc ctgtgtcgca tcatattgca taaaggcacg gtttgggtca
     1681 tcttgcgtta aatcataccc tatcatcgga tattgaccac ttattcccgc cacagaaagt
     1741 aatgttgtcg gcatatcgag ctgactaact aaacggttat ccgttcttgg ctgaatgtgt
     1801 tcgcctaata aaagtgccgg aatatggaag tgttgaattg gcactaaatg ctcgccaacg
     1861 gctcttgaat cgtgatctgc aatcactaaa aacaccgtat cttgccagta ctctgatttt
     1921 ttcgctaact caaagaaatg cccgatagca taatcggcat attttgccgc attatggcga
     1981 gtctgcttcg gttgttcata gagttcaatt ttgccgtccg gaaattcaaa cggatcgtgg
     2041 ttactggaac tgaataccaa actaaaaaac ggtttacctg atttgtgcag ttgattaaaa
     2101 gttccatttg ccttatcaaa cagatcttca tcactcacgc cccaagttgc ggtaaattta
     2161 gggtttttat aatctttttc atcgataatt tgctggaaac cattaccgta gaaaaagctc
     2221 gccatattat caaaatgctt ctccccacca taaataaagg aagtatggta tccttgacgt
     2281 tttaataatt cggcaatact aaaaaagtta gtttggcttt tcgtcagttt taccacagat
     2341 cttgccggtg ttggtgtaaa accagccgtt accgcttcaa ttcctctcac cgaacgagta
     2401 cccgttgcat aaagattggt aaaataccag ccttgttctg aaagtttatc taaatttgga
     2461 gtgagatttt tccctcctag cgaaccgaca aattgagccc ctaaactttc ttctaagata
     2521 atcacaatgt ttttaggctt tccttgataa gtcgctttat tttgcgtgag ggtgggaatt
     2581 tcatctgaaa tataatctga agctggtcga ttacgaacct gcttaacaat gttgaacatt
     2641 tcctccacag acatttttcc atactgttcg gaagatttat cttcatcttt caattgctga
     2701 accgcataaa gcacggaata acctgaattt agcaccagtg aattgacaag cggatcgctt
     2761 gaaaatgcca ccattgccgg attaatccct cgatgggcaa aactggaacg cccaccaaca
     2821 aaaactattg cagcaaccaa taaagccagt agcggacgca ttttccagct cgggaaatgc
     2881 aaatttttgg ttgcccaacc cgataatttc caataaatga tgagagaaat catcgcaatg
     2941 ccaatcccac aaataacagc ccataaatgt ccgttaatca gcattgtgaa cacttctctc
     3001 ggatggacca aatactcaat gaaaagacga tttgggcgaa ggtcaaaggt ttcaataaaa
     3061 ttcggtgtgg cgacttccat aaaaagaata aatacactac cgacagttag ccaaattctt
     3121 aaaacagtct gccaaatcga acccagtaca tttttatgaa aacacaacac gctaaataat
     3181 gccggtaagc caaatagcca acatagcgaa gatatatcaa tgcgaatacc ttgtagtatt
     3241 aatggtatcc atccatcaac agcggatact cgatcagatt gccaaacgcc taacactaaa
     3301 cgggataagg tgaggataac taaattaatc ccaacaaata gtaggattgg aaatagcaca
     3361 gttcttgatt gactgcgttg gtacattttg aatctccttt taaaaaatat cggcatatta
     3421 taattgaata atgacaggat cgcctacttt tatcacacca ctgttttgtg gcacaaggtg
     3481 aataccgaac accggcttac ctttttcatc gcaatgcttt tcttttaagg ttctaaacgg
     3541 ctccgatttg gggtctaact caagcgtgtt taaatcccgt gtaatcaaaa tgcaacgagt
     3601 acataatgcc gctttggtaa atagcacttc gccaatttga acttgttgcc actgctcttc
     3661 ctcaaatgcc acattgccat caatcacaat attcgggcga aaacgagcca tttcgagtgg
     3721 aatcggcgac cattgttgca cttgctcaag cgattttaga gacatcaaac tcaccgggtt
     3781 gctatctgca aaactcattg ggtgatcggc aaaattcgca acaattcgtt ggctttgcga
     3841 gccaagccaa cgtagttgca cagccctacc aaaaatttca ctcagccatt gattgaccgc
     3901 ttctgccgcc actttggcat taaaatgtgt tccccacact tcactactct gctcttccac
     3961 aaaatcttgg taaagtgcaa cgcatttttg accgcttgta tgggtaatca ttacccctgt
     4021 cgcaatcggg aaagccgcta gacggtataa ttccggatct ttacgggcag taataaattt
     4081 gccgttaggc tcggctagca taaattcacg gtcaaaattt aagcctttcg gttcaacaaa
     4141 tgcctgtgaa acttgaatag cttgagttga ttttatcggg taaatattga gctcggtaac
     4201 ttgcatagaa aatcctccat ttggaaatag gataacaata cctgcaagcg gtcgaaaagt
     4261 aaaaggaatt tgcaaatccc ttgtgagaac ggtaaacttg agcaactttt acagcaatag
     4321 gtcagcaaca atggcaaaga aacccactca agattttgaa agcacactta aagaattaga
     4381 agcaatcgtt acccatttgg aatccggtga tttaccgctt gaagatgccc tcaacgaatt
     4441 tgaaactgca atcaaattag tgcaacaagg gcaagaacgg ttacaaaaag ccgagcaacg
     4501 tattcaaatc ttattaaata aaaacgatac tgccccactt agcgattacg aaagagaata
     4561 atcacaatga gctatctatt agcaaccgat ctgactcacc tgcaacaacg gattaaccat
     4621 tttctcgctg aaaaattaaa aacaacggat agcgttggtt cgcctttagt agaagcaatg
     4681 gcttatgcgg tattgctggg cggtaaacgg gtgcgaccgt ttttaatcta tgccaccggc
     4741 agaatgcttg gtgtgccgtt ggaaaaatta gatcatagtg cggcggcaat ggaggctatt
     4801 cacgcttact cgctggttca cgatgattta ccggcaatgg acgatgatag attacgccgt
     4861 ggcaagccaa cctgccatat tgcctttgat gaagctaccg ctattttagc cggcgatgcg
     4921 ttacaaagtt ttgcgtttga actgctggca accgatccgc atttgaccga ccgagaaaaa
     4981 gtcgctcaag ttacagaatt ggcaactgct tccggtgcaa aaggaatgtg cttagggcaa
     5041 agtttggatt tgattgcgga aaataaagct gtcagtttgg aagaacttga attaattcat
     5101 cgcaacaaaa ccggggcatt gatactgtca tccgtgatga tgggattgaa cctttccgaa
     5161 cattcgcaaa atttagccat aaaacaaccg cttgtaaact atgccaaagc gattggctta
     5221 gcctttcagg tgcaagatga tattttagat gtcattggtg ataccgataa aatcggcaaa
     5281 acagtgggtt cagatgaaca cttaaataaa agcacatacc cgaaattgtt gggcttagac
     5341 ggggcaaaac aaaaagccga aatgctttat caaaccgctc tccattcgct ggaacagttg
     5401 cctttcgata ccaccgcatt aaaagagtta gctaatttta ttgttaagcg tgaaaactaa
     5461 gaaaaaacct agccgaagct aggtttttag tgattaaacg tgaggcttgg cttccggcaa
     5521 tgtgccttca atttcatata ccacatcgcc gtctttaaag aaaacatagc caccattttt
     5581 cattttggtc cagttttcat ttttggtaag tggctgagtg gtaataatcg tcactttatc
     5641 agtaattttg gtatattgac taaaatcaat cgacacatca tcatcacgca aggctttgcc
     5701 aaacggtgct ttacggcaaa cgtaatgcag attagttgta caacgggcaa tcatccaatg
     5761 accgttggat aaaacaaaat taaatacccc gtgttccgca attcttgagg taatttgcac
     5821 cacagcatca aaaatctctt tttcgcttgg cttagccgga tacatctctt ttaattccga
     5881 cacaatacgg caaaatgccg cttcggaatc ggttgtacca atcggctggt agtggcactc
     5941 ctcgcatacc ccaacacctt ccactgtgcc gttatgggca aacacccagt tctcgcccca
     6001 taattcacga atgaatgggt gggtattttc caaattcaca tcgccacggg tagctttacg
     6061 aatgtgtgca accacattga gtgatttaat ttgatattgg ctgatcgctt ctgccatcgg
     6121 cgatgatgcc cccgggcgat tatcacgaaa aacccgaacg cctttaccct caaaaaaggc
     6181 aataccgaat ccgtctgtat gatgatctgt aagccctgct ctacggcgga aaccatcaaa
     6241 agaaaatgta atatcggttg gcgtattaca attcattcca agtaattgac acataattga
     6301 cttctttaaa ataaataatt taca
//
LOCUS       DANJWC010000136         6198 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.136, whole genome shotgun sequence.
ACCESSION   DANJWC010000136 DANJWC010000000
VERSION     DANJWC010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6198
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.136"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(11..928)
                     /gene="era"
                     /locus_tag="PWX02_001941"
     CDS             complement(11..928)
                     /gene="era"
                     /locus_tag="PWX02_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013525392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDL1729923.1"
                     /translation="MTEQIQTPAKTYCGFIAIVGRPNVGKSTLLNKILEQKISITSRK
                     AQTTRHRIVGIHTEDQYQAIYVDTPGLHIEEKRAINRLMNRAASSAIGDVDLIIFVVE
                     GTKWTEDDEMVLNKLRSAKAPVLLAINKIDNIKEKEELLPHITELSQKFDFAEILPIS
                     AQRGKNVHILQKFVRQSLRQGEHHFPEEYVTDRSQRFMASEIIREKLMRFTGEELPYS
                     VTVEIEQFKMNERGVYEINGLILVERDGQKKMVIGAGGQKIKTIGIEARKDMERLFDN
                     QVHLELWVKVKAGWADDERALRSLGYMDE"
     gene            complement(1031..1705)
                     /gene="rnc"
                     /locus_tag="PWX02_001942"
     CDS             complement(1031..1705)
                     /gene="rnc"
                     /locus_tag="PWX02_001942"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDL1729924.1"
                     /translation="MQLERLQKKLGYQFHNLDYLIQALTHRSAATKNNERLEFLGDSI
                     LNFTIGKVLYDKFPKSNEGELSRMRATLVREQTLAIIARKFDLGDYLKLGPGELKSGG
                     FRRESILSDCVEAIIAAIYLDQDMAKAMEKVEDWYSDLLKDISPGDSQKDPKTRLQEF
                     LQGRKLPLPEYDVLDIKGEAHNQTFRVTCKVINVEEVFIGIGTSRRKAEQNAAEKALA
                     VVKTKK"
     gene            complement(1751..2710)
                     /gene="lepB"
                     /locus_tag="PWX02_001943"
     CDS             complement(1751..2710)
                     /gene="lepB"
                     /locus_tag="PWX02_001943"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL1729925.1"
                     /translation="MAQFMPIIFLGIIYGIWQWLDSMQLPNTISIILVALVIICGGFW
                     AFYKFSADPRRKQAIVLQQKRLGRELTAEEIAEIQPKSAVGEFFASLFGILFFVTVLR
                     SFLFEPFQIPSGSMEPTLRVGDFLVVNKFSYGIKDPIWQNTLIETGKPERGDVIVFKA
                     PAQPHVDYIKRVVAVGGDKIKYDFATQQLTVTKANGETKVFEYSEGKPNPEFFYHGEM
                     QVERTEKGNVTHQILNHPMAFNYAPYFYQQEGQAEGEWVVPQGYYFVMGDNRDNSEDS
                     RFWGFVPERNIVGKATFIWLSLDKKANEFPTGLRFSRMFTSIN"
     gene            complement(2797..4590)
                     /gene="lepA"
                     /locus_tag="PWX02_001944"
     CDS             complement(2797..4590)
                     /gene="lepA"
                     /locus_tag="PWX02_001944"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDL1729926.1"
                     /translation="MQNIRNFSIIAHIDHGKSTLSDRLIQTCGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLNYKAKDGEIYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAIEMDLEVVPILNKIDLPAAEPERVAEEIEDIVGIDAIDAVR
                     CSAKTGLGIEDVLEEIVKKIPAPEGDAEAPLQALIIDSWFDNYLGVVSLVRVKNGVLK
                     KGDKIKVMSTGQSYNVDRLGIFTPKQVDTAELKTGEVGWVVCAIKDILGAPVGDTLTH
                     HHNPATEVLPGFKKVKPQVYAGLFPISSDDYEAFRDALGKLSLNDASLFYEPETSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVVQTSGETIYVDSPSKLPA
                     VNNIAEIREPIAECNMLVPQEFLGNVITLCVEKRGVQTNMVYHGNQIALTYEIPMGEV
                     VLDFFDRLKSTSRGYASLDYGFKRFQTADMVRVDIMINGERVDALAIIVHRANAAYRG
                     RELVEKMRELIPRQQFDIAIQAAIGNHVIARSTVKQLRKNVLAKCYGGDVSRKKKLLQ
                     KQKEGKKRMKSLGNVEVPQEAFLAILHVGKD"
     gene            4863..6092
                     /locus_tag="PWX02_001945"
     CDS             4863..6092
                     /locus_tag="PWX02_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDL1729927.1"
                     /translation="MQFREINPSPRLLMCPGPTDADPRVLRAMSAPILGQFDPEFTSL
                     MNETMEMERRLFQTKNEQTYVVNSTSRGGLETVLTAAICPGDKVLIPAFGRFGYLLNE
                     ILARSGADITIIEREWGTVFEPEEIEAELKKGGYKAVAIIHGETSTSMMQPLEEIGKI
                     CKQYDAMLIVDTVATLGGVDIRVDEWGIDACIGGTQKCISAPSGTALITYNKRIEEII
                     AKRKRVEKGIRAESDIDGTLPPIPSNYLDLAQLQDYWSPRRLNHHTEATSAQYGVHEA
                     LRIILQEGLEARFARHILNDKALCAGLEAMGLKIFGDRKHKAPVVTAFHIPEGMSGPA
                     LRSDILNHFGIELATSFGPLDGKVIRIGNMGFSSQKRNVLLTLGALEAVLLSHKVKVP
                     SGDAVAAALAVYKEADK"
BASE COUNT         1791 a         1346 c         1137 g         1924 t
ORIGIN      
        1 gacaaaaaaa ctactcatcc atatacccta aacttctcaa tgctctttca tcatccgccc
       61 aaccagcttt tactttcacc caaagctcaa ggtgcacttg gttgtcaaat aagcgttcca
      121 tatcttttcg agcttcaatg ccgatggttt tgattttttg cccccccgca ccaataacca
      181 tttttttctg accatctcgt tctactaaaa tcaagccatt gatttcatac accccacgct
      241 cattcatttt aaattgttcg atttctaccg ttaccgaata aggtaactct tcacctgtaa
      301 aacgcattag tttttcacga ataatttctg atgccataaa gcgttgagag cggtctgtca
      361 catactcttc aggaaagtgg tgctcacctt gacgcagtga ttgacgcaca aatttctgta
      421 aaatatgcac atttttaccc cgttgtgccg aaattggcag aatttcagca aaatcaaatt
      481 tttgtgaaag ctcggtaata tgcggcagaa gttcttcttt ttccttaatg ttatcaattt
      541 tattgattgc caataaaaca ggggcttttg ccgaacgcaa tttgtttaac accatttcat
      601 catcttctgt ccattttgtg ccttccacaa caaagatgat gagatctaca tcgccaatag
      661 cagaacttgc tgcacggttc attaaacgat taatcgcacg tttttcttct atgtgtaagc
      721 ctggggtatc tacataaatc gcttggtatt gatcttcggt gtgaatcccc acaatacggt
      781 gacgggttgt ttgtgctttg cgtgaagtaa tcgagatttt ttgttctaaa atcttattta
      841 ataatgttga tttccctaca tttgggcgac caacaatcgc aataaaacca caataggttt
      901 ttgcaggagt ttgaatttgt tctgtcattt tgttttcctt tcattctgtg gaatgaattt
      961 gatggtgcaa gcgtccacgc ttgtaccatt taattaaatc tttagcacaa gcgaggacgc
     1021 ttgcgctatc ttactttttc gttttaacca ccgctaacgc tttttctgcg gcattttgtt
     1081 cggcttttcg gcggctggtg ccaatgccga taaacacttc ctcgacgtta atcactttgc
     1141 aggttacacg gaaagtttgg ttatgagctt cgcctttaat atctaataca tcatattcag
     1201 gcaatggcaa ttttctacct tgtaaaaact cttgcaggcg ggtttttgga tctttttgtg
     1261 aatcgcctgg actgatgtct ttaagcaaat ctgaatacca atcttccacc ttttccattg
     1321 cttttgccat atcttgatcg agataaatgg ccgcaataat cgcttcaaca cagtccgata
     1381 aaatcgattc tcggcgaaaa ccaccacttt ttagctctcc cggacctaat tttaggtagt
     1441 cgcctaaatc aaatttgcga gcaataatcg ctagagtttg ctcacgcaca agagttgctc
     1501 tcatacggct aagctcacct tcattcgatt ttggaaattt gtcgtacaat actttaccaa
     1561 tagtaaaatt taaaattgaa tcgcctagaa attcaagacg ctcattgttt ttggttgccg
     1621 cactgcggtg tgttaaggcc tgaatgaggt aatcaaggtt atgaaattga taacctaatt
     1681 ttttctgtaa tcgttctagt tgcatagttt atgtgaataa aggggcgtat ctcatacgcc
     1741 cctgttaaga ttaattgatt gaggtaaaca ttctagaaaa acgaagcccc gttgggaatt
     1801 catttgcttt tttatctaaa cttaaccaaa taaaagtggc tttaccaacg atgtttctct
     1861 caggcacaaa gccccagaaa cgactgtctt cactgttatc acggttatca cccatcacaa
     1921 aatagtatcc ttgtggcaca acccattcac cttctgcttg cccttcttgt tggtagaaat
     1981 aaggtgcata gttaaatgcc attgggtgat ttaaaatttg atgagttaca ttaccttttt
     2041 cagtgcgttc tacctgcatt tcaccgtgat agaaaaactc aggatttggc ttgccttcgc
     2101 tgtattcaaa tacctttgtt tcgccattgg ctttagtaac cgttaattgc tgagttgcaa
     2161 aatcatattt aattttatcg ccacctactg ccaccacacg cttaatataa tcaacgtgtg
     2221 gttgtgctgg tgctttgaat acaatcacat cgcctcgttc tggtttgccc gtttcaatca
     2281 aggtgttttg ccagatagga tctttaatgc cgtagctgaa tttatttacg actaagaaat
     2341 cgcctacacg caaggttggc tccattgaac cacttggaat ttgaaatggt tcaaataaga
     2401 acgagcgaag tactgtaaca aagaataaaa tgccgaaaag tgaggcaaaa aattcgccta
     2461 ctgcggattt aggttgaatt tccgctattt cttcagcggt taattctctg cctaaacgtt
     2521 tttgttgtag aacaatcgcc tgtttacgtc ttgggtcagc actgaatttg taaaacgccc
     2581 aaaatccacc acaaataata accaaggcaa ctaaaataat ggaaatagta tttggcagtt
     2641 gcattgaatc aagccattgc caaataccat aaataatacc tagaaaaata attggcataa
     2701 attgtgccac tgaatgctcc tttttattca aaattgcaaa gttttctcaa aatatgaccg
     2761 cttgcaagcg gtcgtatttt acacgaatat tgacaattaa tccttaccca catgaagaat
     2821 tgccaaaaat gcttcttgtg gcacttctac gttaccgaga gatttcattc gttttttacc
     2881 ctctttttgt ttttgtagca gtttcttctt acgacttaca tcaccaccgt aacattttgc
     2941 taatacgttt ttacgcaact gttttaccgt tgagcgagca ataacgtggt taccaattgc
     3001 cgcttgaatt gcgatgtcaa attgttggcg aggaatcagc tcacgcattt tttccactaa
     3061 ttcacgtcca cgatacgccg catttgcacg gtgaacgata atcgccaacg catcaacacg
     3121 ctcgccgtta atcataatat ccacacgcac catatcagcc gtttggaaac gtttaaaacc
     3181 gtaatcgagt gaagcataac cacgagaggt tgattttaag cggtcgaaga agtctaacac
     3241 tacttcgccc attgggattt cgtaagttaa ggcgatttgg ttaccgtgat agaccatatt
     3301 ggtctgtacg ccacgttttt ccacacaaag ggtaatcacg ttgcctagga attcttgtgg
     3361 cacaagcata ttacattctg caatcggctc acgaatttct gcaatattgt tgactgctgg
     3421 gagtttcgat gggctatcca catagatcgt ttcgccactg gtttgcacca cttcgtaaac
     3481 tacggtcgga gcggtagtaa tcaaatcaag atcgtattca cgctctaaac gctcttgaat
     3541 gatctccata tgtaataagc ctaagaagcc gcagcggaaa ccaaagccta acgcacttga
     3601 agtttctggc tcatagaaca atgacgcatc atttaggctt aatttgccta aagcatcacg
     3661 gaaggcttcg tagtcgtctg aactaatcgg aaataaccct gcataaacct gtggttttac
     3721 tttcttaaag cccggtaata cttcagttgc tgggttgtgg tggtgggtta atgtatcgcc
     3781 cactggtgcc cctaaaatat ctttaatcgc acaaactacc caacctactt ccccagtttt
     3841 aagttctgcg gtatccactt gttttggcgt gaaaatacca aggcgatcta cattgtaaga
     3901 ctgccctgta ctcatcactt taattttatc gccttttttc agcacgccat ttttcacacg
     3961 cactaaagac acaacgccta aatagttatc gaaccaagag tcgataatta acgcttgtaa
     4021 tggtgcttcc gcatcgcctt ctggtgcagg aattttcttc acgatttctt ctaaaacatc
     4081 ttcaatgcct aaacctgttt tagctgaaca gcgtactgca tcaatggcat caatgccgac
     4141 aatatcttca atctcttctg ccacacgctc aggctcggct gctggtaaat cgattttatt
     4201 taaaattggc acaacctcta aatccatctc aattgcggta taacaattcg ccaacgtctg
     4261 tgcttccacg ccctgccctg catcaaccac taataaagca ccttcacaag cagcaagcga
     4321 gcgagaaact tcatatgaga aatccacgtg ccctggcgta tcaataaagt tgagctgata
     4381 aatttcgcca tctttcgctt tatagtttaa ggttacactt tgtgctttaa tagtaattcc
     4441 acgctcacgc tcaagatcca ttgaatctaa tacttgagcc tccatttcac gatctgataa
     4501 gccaccacaa gtttgaatta agcggtctga taaagtcgat ttgccgtgat cgatatgagc
     4561 gataatcgaa aagttacgaa tattttgcat tttcatcggg cagttaatgt cctgttttgt
     4621 tatttgttta tcaaaaatta atcggggaat tgtacttgaa atacaaggtt ttgcaaagaa
     4681 aacaaatagc aataagcggt tattttttcc taaaaatgtg caaatttctt gagaaaaatg
     4741 accgcttgca ctcaccctaa ttgcaaataa tttctctaaa atataaaaaa cattgagcaa
     4801 tcacaaattt ttattgtaga atccataagt ttttttaatt cattcgatta taggaaataa
     4861 ttatgcaatt cagagaaatt aatccctccc ctcgtttatt aatgtgccct ggcccaacag
     4921 atgctgaccc acgtgttttg cgtgcaatgt ctgcacccat tttagggcaa tttgaccctg
     4981 agtttacctc attaatgaat gaaacaatgg aaatggaacg ccgtttattc caaaccaaaa
     5041 atgagcaaac ttatgtagtc aactccacct ctcgtggtgg cttagaaact gttctaaccg
     5101 cagcgatttg cccaggcgat aaagtgttaa ttcctgcatt tggtcgtttt ggttatttat
     5161 taaatgaaat tttagctcgt agcggtgcag atattaccat tatcgaaaga gagtggggaa
     5221 ccgtatttga accagaagaa attgaagcag agttgaaaaa aggcggctat aaagcagttg
     5281 ctattattca cggcgaaacc tcaacctcta tgatgcaacc gttagaagag atcggcaaaa
     5341 tctgtaaaca atatgatgca atgttaattg tagataccgt tgcaacctta ggcggtgtag
     5401 atattcgtgt ggatgaatgg ggcattgatg cttgtatcgg cggtacacaa aaatgtattt
     5461 ccgccccatc aggcacagct ttaatcactt ataacaaacg gattgaagag attattgcaa
     5521 aacgtaaacg tgtggaaaaa ggcattcgtg cagagtctga tatagatggc acattaccgc
     5581 caattccaag taactactta gacttggcac agctacaaga ttactggagc ccacgccgtt
     5641 taaatcacca cacagaagcc acttctgcac aatatggtgt acacgaagcg ttacgcatta
     5701 ttttacaaga aggcttagaa gctcgttttg ctcgccatat tttaaacgac aaagcattat
     5761 gtgcaggttt agaagcaatg ggtttgaaaa tttttggcga tcgcaaacat aaagcccccg
     5821 ttgtaacggc attccacatt ccagaaggta tgagtggccc agcattgcgt agtgatattt
     5881 taaaccactt cggtattgaa ttagccacct cattcggccc cttagatgga aaagtaatcc
     5941 gtatcggtaa tatgggtttc tcaagccaaa aacgtaacgt acttttaacc ttaggtgcgt
     6001 tagaagccgt acttttaagc cataaagtaa aagtgccttc aggcgatgcg gttgcagctg
     6061 ccttggcagt ctataaagag gctgataaat aaccaccttc ctaaatttac aatacaactc
     6121 actaataatt aatactgtgg atatttatcc acagtaaaac cacagaaact caaaatattt
     6181 tcacaaattt aatctttt
//
LOCUS       DANJWC010000137         6172 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.137, whole genome shotgun sequence.
ACCESSION   DANJWC010000137 DANJWC010000000
VERSION     DANJWC010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6172
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.137"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            121..2043
                     /locus_tag="PWX02_001946"
     CDS             121..2043
                     /locus_tag="PWX02_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HDL1729928.1"
                     /translation="MKLIEIENLNKYFGLGQTQAHILKNINLSIERGDFVAIIGASGS
                     GKSTLMNIIGCLDTASSGICRIDGKETQTMSADELSDLRQRKLGFIFQRYNLLSALNA
                     NENVALPAIYAGMDSSSRKKRADTLLTKLGLSDKTQHKPNELSGGQQQRVSIARALMN
                     GGEIILADEPTGALDSKSGETVLEILRDLHHEGHTIIMVTHDPNIAAQASRVVEIKDG
                     QIIRDEHQRADVQETALNKTTTQKTRFWAQLFESFKMATSAIMAHKMRAILTMLGIII
                     GITSVISVVALGRGSQQQILSNINNLGTNTMDIMNGTGFGDRRANLTKNLTISDALML
                     GRQSYVESTTPSSNVSATLIYGNTAVTGSVRGVGEQFIDVKGLKLISGRFFSAEEVKN
                     VAQIIVIDPNTQKDLGLTEPVEGKTILVDKKPLQIIGVAQQSNNMNQSSLTLWAPYTT
                     IMQRISGAKQIDSLTVKIKDDIESQTAEKSITELLVAKHGKKDFFIMNSDTIKQTITS
                     TTNTMTLLISSIALISLIVGGIGVMNIMLVSVTERTREIGVRMAIGAKQRNILQQFLI
                     EAVVICLIGGVIGILLASAIIWAFNTLGSNFKMMLSPESVVIAVLCSTLIGVVFGYIP
                     ARNASQLNPITALAQE"
     gene            2126..2896
                     /locus_tag="PWX02_001947"
     CDS             2126..2896
                     /locus_tag="PWX02_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDL1729929.1"
                     /translation="MTITNTELEQILNTKLNSSAINDYAPNGLQVEGKREIKKIITGV
                     TATLPLIEKAIERNADAILVHHGYFWKSETPCIRGMKGKRIKQLLVNDINLFGYHLPL
                     DIHPELGNNAQLAKHLGVKNLQGLEDRPNSIPMYGELENPISANELKQKLEKTLQRTV
                     ILCDEFVHSPQKMIKTIGICSGGGQGYIDLAFEKGCDAFISGEISEQTTHSAREQGIY
                     YFACGHHATERDGVKALGEWLAVEYGLDVEFIDIDNPA"
     gene            complement(2977..4107)
                     /gene="nrdB"
                     /locus_tag="PWX02_001948"
     CDS             complement(2977..4107)
                     /gene="nrdB"
                     /locus_tag="PWX02_001948"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDL1729930.1"
                     /translation="MAYTTFSQHKNDQLKEPMFFGQNVNVARYDQQKYEIFEKLIEKQ
                     LSFFWRPEEVDVSQDRIDYAQLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWTFSETIHSRSYTHIIRNIVNDPSIVFDDIVTNEEIIKRAKDISAYYDD
                     LIRDSQLYSLYGEGSYQVDGKTCEVSLRSLKRQLYLCMMSVNALEAIRFYVSFACSFA
                     FAERQLMEGNAKIIKFIARDEALHLTGTQHILNIMTYGNDDPEMAEIARECRQEAYEL
                     FLSAAEQEKAWAEYLFKDGSMIGLNRDILVQYIEYITNIRMQAVGLPLPFKARSNPIP
                     WINAWLVSDNVQVAPQEVEVSSYLVGQIDAKVDSRDFGDFEL"
     gene            complement(4134..>6172)
                     /gene="nrdA"
                     /locus_tag="PWX02_001949"
     CDS             complement(4134..>6172)
                     /gene="nrdA"
                     /locus_tag="PWX02_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDL1729931.1"
                     /translation="YLAARLAIFHLRKKAYGQFEPPHLYEHVKKLVALGKYDADLLSD
                     YTPEEWETMNGFIDHWRDMTFSYAAVKQLEGKYLVQNRVSGEIYESAQFLYLLVAACL
                     FSKYPTASRLDYIRRFYDATSTFKISLPTPIMAGVRTPTRQFSSCVLIECDDSLDSIN
                     ATSAAIVKYVSQRAGIGINAGAIRALGSPIRGGEAFHTGCIPFYKHFQTAVKSCSQGG
                     VRGGAATVYFPIWHLEAESLMVLKNNRGVEENRARHMDYGVQINKMMYQRLIKGGEIS
                     LFSPSDVPGLYEAFFADQAKFEQLYLQYEQDPSIRQRKVKAVELFSLLMQERASTGRI
                     YIQNVDHCNTHSPFNPRVAPIRQSNLCLEIALPTKPLAHFYDEQGEIALCTLSAFNLG
                     TLSNLDELEELADLVVRALDALLDYQDYPVLAARNSSLNRRALGVGVINYAYYLAKNQ
                     VRYSDGSANNLTHRTFEAIQYYLLKASMNLAKEQGACAYFDQTTYAKGLLPIDTYKKE
                     IDELTQEPLHYDWEQLRQDILQFGLRNSTLTALMPSETSSQISNATNGIEPPRGYISV
                     KASKDGILKQVVPEYERLKAYYELLWDIPNMDGYLHLVGIMQKFVDQAISANTNYDPN
                     RFAEAKVPMKVLLKDLLTAYKYGLKTLYYQNTRDGAEDVQDDLDEGCAGGACKI"
BASE COUNT         1934 a         1290 c         1183 g         1765 t
ORIGIN      
        1 ctattcattt agataaaagt aatatgggag gattaaaaat cgaactgaaa cttccccttt
       61 ggcgttccta aggcaaaaag catatcgttt gtaaaaaaca gtcgaaaaat aaccgcttgt
      121 atgaaattaa ttgaaataga aaatctcaat aaatactttg gcttgggaca aacccaagcc
      181 catattctaa aaaatatcaa tctcagtatt gaacggggtg attttgtcgc aattataggt
      241 gcttcaggtt ctggcaaatc taccttaatg aacattattg gctgtttaga tactgcaagc
      301 tcaggtattt gccgtattga tggcaaagag acccaaacaa tgtctgcaga tgaactgtct
      361 gatttacgcc aacgtaaact ggggtttatt ttccaacgct ataatctcct ttcagctttg
      421 aatgcaaacg aaaatgttgc cctacctgca atttacgccg gaatggatag ctcatcacgc
      481 aaaaaacgag ctgatacgtt attaacaaag ttaggattat cagataaaac ccaacataag
      541 ccgaatgagt tatctggggg gcaacagcaa cgagtcagca ttgcacgagc cttaatgaac
      601 ggcggtgaaa tcattcttgc agacgagcct accggtgcat tagattccaa aagtggcgaa
      661 actgtattgg aaatcttgcg agatctacac cacgaaggtc ataccattat tatggtgaca
      721 cacgacccaa acattgccgc tcaagccagc cgtgtagttg aaattaaaga tggacaaatc
      781 attcgagatg aacaccaacg tgctgatgtt caggaaacag ctctcaataa aacgacaaca
      841 caaaaaactc gcttttgggc tcaactgttt gaatccttca aaatggcaac cagtgcaatt
      901 atggcacata aaatgcgggc aatcctcacg atgctgggca ttattattgg cattacctct
      961 gtgatttccg tagtcgctct cggtcgaggc tcgcaacaac aaatcctttc taatatcaac
     1021 aacctaggca caaatacgat ggatattatg aatggaaccg gctttggcga tagaagagcc
     1081 aatctgacta agaatttaac catcagtgat gccttaatgt tggggcggca aagctatgtg
     1141 gaaagcacta ctccttcaag caatgtcagt gccacattga tttatggtaa taccgcagta
     1201 accggttcag tacgaggtgt tggagagcaa tttattgatg tgaaagggtt aaagctgatt
     1261 tcagggcgtt ttttcagtgc cgaggaagta aaaaatgtgg cacagattat tgtaattgac
     1321 cctaacactc aaaaagattt ggggctgaca gagcctgttg aaggtaaaac gattctagtg
     1381 gataaaaaac cgttacagat tattggtgtg gcacaacagt caaataatat gaaccaaagt
     1441 agccttaccc tgtgggcgcc ttataccact attatgcaac gcatttcagg tgcaaaacag
     1501 atagattcac tcacggttaa aattaaagat gatattgaaa gccaaactgc cgaaaaaagc
     1561 attaccgaat tgttggtagc taaacacggc aaaaaagatt tctttattat gaatagtgat
     1621 acgatcaaac aaaccattac cagtaccacc aatacaatga ccttgctgat ttcctctatc
     1681 gcccttattt cgctgattgt cggcggtatc ggggtaatga atattatgtt ggtttccgtc
     1741 accgagcgaa cccgagaaat tggtgtccga atggcgattg gtgcaaaaca acgcaatatt
     1801 ttgcaacaat ttttaattga agcggtggtt atttgcctga ttggtggtgt aataggcatt
     1861 ttgctcgcaa gtgctattat ttgggcattt aataccttag gctcgaattt taaaatgatg
     1921 ctttcacctg aatcggttgt gattgccgta ctctgctcta ctcttatcgg tgtggtattt
     1981 ggctacattc cggcaagaaa cgcctcccaa ttaaacccaa ttactgctct ggcacaagag
     2041 taaatgcgac aactatctgc tacactgtag ggcaaaaaat tttttgccct tacatccatt
     2101 attatgccaa gtttaaggag ataaaatgac aataaccaat actgaactag aacaaatact
     2161 gaacaccaaa cttaacagct ccgccattaa cgactatgcc ccaaatggct tgcaagttga
     2221 agggaaaaga gaaatcaaaa aaatcattac cggtgtgact gccacacttc ccctgattga
     2281 aaaagcgatt gagagaaacg ccgatgcaat tttagtccac cacggttatt tttggaaaag
     2341 cgaaacccca tgcattcgag gaatgaaagg taaacgcatt aaacagcttt tagtgaatga
     2401 catcaatctc tttggttatc acctaccact ggatattcac cccgaactag gtaacaacgc
     2461 ccagcttgct aagcatttag gcgtaaaaaa tctacaaggt ttagaagaca gacctaattc
     2521 aattccaatg tacggtgaat tagaaaatcc gatttccgcc aatgaactaa aacaaaaact
     2581 tgaaaaaacg ttacaacgca ccgttatttt atgtgatgaa tttgttcata gcccacaaaa
     2641 aatgattaaa acaatcggga tttgctccgg cggaggacaa ggctatattg atttagcctt
     2701 tgaaaaaggc tgtgatgcct ttattagcgg cgaaatttcc gaacagacca cccactctgc
     2761 ccgtgaacaa ggaatctatt attttgcttg tggtcatcac gccacggaac gagatggcgt
     2821 aaaagcctta ggtgaatggt tagccgttga atatggttta gatgttgaat ttattgatat
     2881 tgataaccct gcataaaaaa tacaagcggt tgaatttcct gaaaattttg caatttgcaa
     2941 aaaatcacta aaaatcaacc gcttgtatat catagtctaa agttcaaaat cgccaaaatc
     3001 acggctatct actttagcat caatctggcc aaccaaataa gaactgactt ccacttcttg
     3061 tggtgctacc tgtacattat cggaaaccaa ccacgcattg atccacggaa tggggttgga
     3121 acgtgcctta aatggtaatg gtagtccaac agcctgcatc cgaatattag tgatatattc
     3181 aatatattgc accaaaatat cccgatttag ccctatcatc gaaccgtctt taaacaggta
     3241 ttccgcccac gctttttctt gttcagctgc acttaaaaaa agctcgtagg cctcttggcg
     3301 acattcacgt gcaatttctg ccatttcagg atcatcattg ccataggtca taatattcaa
     3361 tatatgctga gtgccggtaa gatgtaatgc ttcatcacgt gcaataaatt tgatgatttt
     3421 ggcatttcct tccataagct gccgttccgc aaaggcaaat gagcaagcaa aagagacata
     3481 aaaacgaatt gcttcaagcg cattcacact catcatacag agataaagtt gtctttttag
     3541 actacgcaaa ctgacctcac aggtttttcc atcaacctga taactgcctt caccatacaa
     3601 actatagagt tggctgtcac gaatcaaatc atcataatag gcggaaatat ctttggcacg
     3661 cttaataatt tcttcgtttg tgacaatatc atcaaatact attgatggat cattgacaat
     3721 attacggatg atatgtgtgt aagaacgact gtgaattgtt tcgctaaatg tccaagtttc
     3781 aatccaggtt tcaagctcgg ggatagacac taatggcaaa agcgccacat tcgggctacg
     3841 cccttgaatg gaatccaaca aggtttggta ttttaaattg ctgataaaaa tgtgcttttc
     3901 atgttcaggc agttgggcat agtcaatgcg gtcttgcgac acatccactt cttccggtcg
     3961 ccagaaaaat gagagttgtt tttcaatcag tttttcaaaa atttcgtatt tttgttgatc
     4021 ataacgcgcc acattcacat tttgtccaaa aaacatgggt tcttttagtt gatcattttt
     4081 atgttgtgaa aacgtagtat atgccatgtt attctccttt atttctgttc ttattaaatt
     4141 ttacaagcac caccagcaca gccttcatct aagtcgtctt gcacatcttc tgctccatca
     4201 cgggtatttt gatagtaaag ggtttttaac ccgtatttat acgccgttaa tagatctttt
     4261 aataatactt tcatcggtac tttagcttct gcaaagcggt ttgggtcgta atttgtattc
     4321 gccgaaatcg cttggtcaac aaatttctgc ataatcccaa ccaaatgcaa atagccgtcc
     4381 atattaggaa tatcccaaag cagttcatag tatgctttta aacgttcata ctccggcaca
     4441 acctgcttta aaataccgtc ttttgatgct ttaacactaa tatagccacg cggaggttca
     4501 attccattgg tggcattcga aatttgcgac gaagtctccg atggcattaa ggcagttaaa
     4561 gtggaattgc gcaagccaaa ttgcagaata tcctgacgta gttgttccca atcataatga
     4621 agcggttctt gagttaattc atcaatttct tttttataag tatcgatagg gagtaaccct
     4681 ttggcataag tggtttgatc aaaataagca cacgcccctt gttctttggc taaattcatc
     4741 gatgctttta ataaataata ctgaatcgct tcaaaggtac ggtgagttaa attattagca
     4801 ctgccatcgg aataacgcac ttggttttta gctaaatagt aagcatagtt aatcacgcct
     4861 acacctaatg cgcggcgatt taatgagctg ttacgtgcag ctaataccgg ataatcttga
     4921 taatctaata gggcatctag cgcacgaacc actaaatcag ccaattcttc taactcatct
     4981 aaattgctga gtgtgcctaa attaaatgcc gataatgtac ataatgcgat ttccccttgt
     5041 tcatcataaa aatgtgccag tggcttagtt ggcaaagcaa tttctaagca gaggttcgat
     5101 tggcgtatcg gtgcgacacg aggattaaat gggctatgag tattacaatg atcgacattc
     5161 tgaatataaa ttcgccctgt tgaagcccgc tcttgcatca gtaatgaaaa caattctaca
     5221 gctttaactt tacgttgccg aatgctcgga tcttgctcat attgcaaata aagttgctca
     5281 aatttagctt gatcagcaaa aaacgcttca tataaccccg gcacatctga tggactaaat
     5341 aaactaatct caccgccttt aattaaacgt tgatacatca ttttattaat ctgtaccccg
     5401 taatccatat ggcgagcacg gttttcttcc acaccacggt tattttttaa taccattaag
     5461 ctttctgctt ccaaatgcca aatcggaaaa tacacggtag ccgctccacc gcgaacacca
     5521 ccttgagagc aagactttac tgcagtttga aaatgtttat aaaagggaat acagccggta
     5581 tgaaatgctt ctccaccacg aatggggcta cctaatgccc gaattgcgcc ggcattaata
     5641 ccgatcccag cacgctgtga aacatattta acgattgcag ccgaggttgc attgatggaa
     5701 tctaagctat catcacactc aattaacaca caggaactaa attgacgagt aggtgtacgc
     5761 actcctgcca taatcggtgt tggcaaagaa attttgaaag tggaagtcgc atcataaaaa
     5821 cggcgaatat aatctaagcg gcttgctgtt ggatacttag aaaataaaca ggcagctacg
     5881 agtaaataca agaattgagc cgattcataa atctctccgc taacacgatt ctgcactaaa
     5941 tattttcctt ccaactgctt tactgctgca taggaaaacg tcatatcccg ccagtgatca
     6001 ataaatccat tcattgtctc ccattcttcg ggcgtatagt cgctcagcaa gtctgcatca
     6061 tatttaccta gcgcaactaa ttttttaacg tgctcatata aatgcggtgg ctcaaattgt
     6121 ccataagctt ttttgcgtaa gtggaaaatc gccaagcgtg cggctaaata tt
//
LOCUS       DANJWC010000138         6093 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.138, whole genome shotgun sequence.
ACCESSION   DANJWC010000138 DANJWC010000000
VERSION     DANJWC010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.138"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            22..1008
                     /gene="pheS"
                     /locus_tag="PWX02_001950"
     CDS             22..1008
                     /gene="pheS"
                     /locus_tag="PWX02_001950"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDL1729932.1"
                     /translation="MQHLKDLTNKAREALDKIDQNLEALEEFRVEYFGKKGHFTTLMQ
                     ELRNIAAEERPAIGAKINEAKQVVLDVLNAKKEEWEQAVLNAKLANESIDVSLPGRKT
                     ELGGLHPVSITIERVVKFFSELGFTVANGPEIETDYYNFDALNIPDHHPARADHDTFW
                     FDANRLLRTQTSGVQIRTMENVKPPIRIVAPGRVYRNDYDQTHTPMFHQIELLYVDKK
                     ANFTELKGLIHDFLKAFFEEDLQVRFRPSFFPFTEPSAEVDVMRQNGKWLEVLGCGMV
                     HPNVLRNVGIDPEEYSGFAVGMGVERLTMLRYNVTDLRSFFENDLRFLKQFK"
     gene            1056..3443
                     /gene="pheT"
                     /locus_tag="PWX02_001951"
     CDS             1056..3443
                     /gene="pheT"
                     /locus_tag="PWX02_001951"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDL1729933.1"
                     /translation="MKFNESWLREWVNPAISTEQLCDQITMLGLEVDDVEPVAGAFSG
                     VVVGEVVECAQHPDADKLRVTKVNVGGERLLDIVCGAPNCRQGLKVACAVEGAVLPGD
                     FKIKKTKLRGQPSEGMLCSYSELGIKEDHSGIIELPADAPIGQDFREYLNLNDVAIEI
                     SLTPNRADCLSIAGIAREVGVINRVDVKAPTITDVKATISDKVSVELQAPEACPRYLA
                     RVVKNVNVKATSPLWLQEKLRRCGIRSIDPIVDITNLSLLELGQPMHAFDAAKIEGGI
                     QVRMAKEGEELVLLDGTNAKLQSNTLVIADSKGALAMAGIFGGEASGVSENTKDVVLE
                     SAFFASLAITGRARQYGLHTDASHRFERGVDPELTRQAMERATALLLEICGGEASEIV
                     EAVSQENLPKRNTVTLRRSKLDSVIGHHIDDATVTDILTRLGLNVTFADNSWTAIAPS
                     WRFDIEIEEDLIEEIARIYGYNSIPNNSPLAHLTMKGTPEKLLEANRIRMALVDSDYQ
                     EVITYSFVDPKKQSLLHPQQEALILPNPISSEMSAMRVSLLTGLLDTIVYNQSRQQNR
                     VRIFEAGLRFIPDSNAESGVRQEFVFGAAIVGDKRPVHWENKSEAADFFDLKGDIERI
                     LSLTAIRHDLQFVAKQFPALHPGQSAAIMLDGKEIGFIGTVHPSIVQKFGIKGKPIVC
                     EILASAIAERPVTQAGEISKFPANNRDIAVVVDESVPAGEVLEACRKAGGNKLVAVNL
                     FDVYRGSNLESGKKSLAISLTVQDNEKTLEENEINAVIQAVLAELATRFNAVLRD"
     gene            3501..3797
                     /locus_tag="PWX02_001952"
     CDS             3501..3797
                     /locus_tag="PWX02_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HDL1729934.1"
                     /translation="MALTKIEIAENLIEKFGLEKRVAKQFVELFFEEIRSSLENGEEV
                     KLSGFGNFSLREKKARPGRNPKTGENVAVSARRVVVFKAGQKLRERVEQASSQS"
     gene            3836..4357
                     /locus_tag="PWX02_001953"
     CDS             3836..4357
                     /locus_tag="PWX02_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL1729935.1"
                     /translation="MKLTKRWLSLGCLLATTAMLVACSSSDTTSKSQVKSKVYRSIYQ
                     SDLSDPIMAISRLSEHQHEWKGTRYRLGGNSKAGIDCSGFMQVTFKDLFGIDLPRTTT
                     EQAKAGERISKDELRTGDLVFFKTGRGPNGKHVGVYVKNGQFLHASTKGGVIYSDLDS
                     PYWSKAFWQARRL"
     gene            4373..5416
                     /gene="degS"
                     /locus_tag="PWX02_001954"
     CDS             4373..5416
                     /gene="degS"
                     /locus_tag="PWX02_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HDL1729936.1"
                     /translation="MLKKIGQAVIFGLFCAILILFVAPIIQSKGSSFSLSAFTQKEIV
                     SYNAAVKIASPAVVNVYSRNFSSENETFEVKNLGSGVIMSSDGYILTNRHVIEDADQI
                     VIALQNGAISTNVKLVGDDSLTDLAVLKIEGENLPTIPQNPERKLQIGDVVLAIGNPL
                     NLGQSITQGIVSAIGRKTLTESGRQNFIQTDVSINQGNSGGALINTAGELIGINTLRF
                     GKNTNELSEGLNFAIPINLANQIMSKIIKDGRVIRGYFGVSTDLLDTAKMQGYEKGVI
                     IVNVDKNGPAAKAGIQAGDLVLKVGDIEAFSPSQMMEELAEMKPYTTVKVLVQRDEQL
                     LNFDVTITELQTQ"
     gene            5425..>6093
                     /gene="cmk"
                     /locus_tag="PWX02_001955"
     CDS             5425..>6093
                     /gene="cmk"
                     /locus_tag="PWX02_001955"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDL1729937.1"
                     /translation="MKKIVITVDGPSGAGKGTLCHALAEKLGFDFLDSGALYRITALS
                     AIKKEVPLDDEEKLAEVGRNLDIQFLPENGEIQVILEGENVGDLIRTAEAGQNASKVA
                     AFPLVREALLERQRAFSSEKGLIADGRDMGTVVFPDAQIKLFLDASAEERTKRRVKQL
                     QEKGFNANFDEILAEIKERDFRDRNRAVAPLVPAKDALLLDSTHLSIEDVINQALNHI
                     AQYIK"
BASE COUNT         1887 a         1020 c         1444 g         1742 t
ORIGIN      
        1 attgagttta gaggataggt aatgcaacac ctaaaagatt taactaataa agcacgtgaa
       61 gctttagata agattgatca aaatttagag gctttagaag agtttagagt cgaatatttt
      121 ggtaaaaaag ggcattttac aacgttaatg caagaattac gcaatattgc agcggaagaa
      181 agaccggcaa tcggagcaaa aattaatgaa gcaaagcaag ttgtgcttga tgtgttaaat
      241 gccaaaaaag aagaatggga acaagctgta ttaaatgcaa aattagctaa tgagagcatt
      301 gatgtttcac tgccggggcg taaaactgaa ttaggtggtt tgcaccctgt ttctatcact
      361 attgagcgtg tggtgaaatt tttctctgaa ttaggtttta ccgtagcaaa cggacctgaa
      421 attgaaaccg attactataa cttcgatgcg ttaaacattc cggatcatca tccggctcga
      481 gcggatcacg atacgttttg gtttgatgcc aatcgtttat taagaaccca aacctcgggt
      541 gtgcaaatcc gtacaatgga aaatgtaaaa ccgcctatcc gtattgtggc accggggcgt
      601 gtatatcgta atgactacga ccaaacccac acaccgatgt tccaccaaat cgaattgtta
      661 tatgtggata aaaaagcaaa ctttaccgag ttaaaaggct taattcacga ttttctcaaa
      721 gcattctttg aagaagattt acaagtgcgt ttccgcccgt cattcttccc attcactgag
      781 ccttctgcag aagtagatgt aatgcgtcaa aacggcaaat ggttagaagt gttaggttgc
      841 ggtatggtac acccgaatgt gttacgcaat gtagggattg accctgaaga atatagcggt
      901 tttgctgtgg gaatgggcgt cgagcgttta acgatgttac gctacaatgt cacggattta
      961 cgttcattct ttgaaaatga cttacgtttc ttaaaacaat ttaagtaata caagcggtta
     1021 aaaatttaga aaattttaca aataggatag aaataatgaa attcaatgaa tcttggttgc
     1081 gtgaatgggt aaacccagca attagcacag aacagttatg cgaccaaatt acaatgttag
     1141 ggttagaagt tgacgatgta gagccggtag ctggtgcgtt ttcgggtgta gttgtgggtg
     1201 aagtggtgga atgcgcccag cacccggatg ctgacaaatt gcgtgtcact aaagtgaatg
     1261 ttggtggcga acgtttatta gatattgttt gtggtgcacc aaactgccgt caaggtttaa
     1321 aagtggcttg cgctgttgaa ggtgcagtgc taccgggcga tttcaaaatt aagaaaacca
     1381 aattgcgtgg tcagccttca gaggggatgt tatgttctta ctcagaactt gggattaaag
     1441 aagatcatag cggtattatt gaattacctg ctgatgcacc tattggtcaa gatttccgtg
     1501 aatatttaaa tttaaatgat gtggcgattg aaatcagcct tacgccaaac cgtgccgact
     1561 gcttaagcat tgccggcatt gctcgtgaag tgggggtgat taaccgtgtc gatgtgaaag
     1621 ctccaactat tacagatgta aaagccacga tttcagataa agtttcagtg gaattacaag
     1681 caccggaggc ttgccctcgc tatttagccc gtgtggtgaa aaatgtgaat gtcaaagcga
     1741 catcgccact ttggttacaa gagaaattac gccgttgtgg gattcgctca atcgatccaa
     1801 tcgttgatat tactaacttg agccttttag aattagggca gccaatgcac gcctttgatg
     1861 cagcgaaaat tgaaggtggc attcaagtgc gtatggcaaa agaaggcgaa gaattggtat
     1921 tgctagatgg cacaaacgct aaattacaat cgaatacctt agtgattgct gatagcaaag
     1981 gggcattggc tatggcaggt attttcggtg gcgaagccag cggggtgagc gagaatacga
     2041 aagatgtggt gttagagtct gcattttttg cttcattagc gattaccggt cgcgctcgtc
     2101 aatatgggtt acataccgac gcatcacacc gttttgagcg tggggttgat ccagaattaa
     2161 ctcgtcaagc aatggagcgt gcaacggcat tattactgga aatttgcggc ggtgaagcaa
     2221 gtgaaattgt ggaagccgtg agccaagaaa acctaccaaa acgcaatact gtcacgctta
     2281 gacgcagtaa attagattcg gtgattggtc atcatattga cgatgcaacg gtaaccgata
     2341 ttttaacccg cttaggcttg aacgtaacat ttgcggataa tagttggacg gcaattgctc
     2401 caagttggcg ttttgatatt gaaattgaag aagatttaat tgaagaaatc gcgcgaattt
     2461 atggctacaa cagtatccca aataattcgc cacttgctca tttaacgatg aaaggtacgc
     2521 cggaaaaatt actggaagca aaccgtattc gtatggcatt ggtagatagt gattaccaag
     2581 aagtgattac ttatagcttt gttgatccga aaaaacaatc attattgcac ccacagcaag
     2641 aagccttgat tttaccaaac ccgatttcaa gtgaaatgtc ggcaatgcgt gtttctcttt
     2701 taaccggttt gttggacact atcgtgtata accaaagccg tcagcaaaac cgtgtgcgta
     2761 tttttgaagc aggtctacgt tttattccgg atagcaatgc ggaatcaggc gtgcgtcaag
     2821 aatttgtttt tggtgcggcg attgtaggcg ataaacgtcc ggtacattgg gaaaacaaat
     2881 cagaagcagc cgatttcttt gatttaaaag gcgatattga gcgtattctt tctttaaccg
     2941 ctatccgcca cgatctgcaa tttgtcgcaa aacagttccc agcattacac ccggggcaat
     3001 cggctgcgat tatgcttgat ggcaaagaaa ttggttttat cggaactgtt cacccgtcaa
     3061 ttgtgcaaaa gtttggcatt aaaggcaaac cgattgtatg tgaaattcta gcatcagcta
     3121 ttgcagagcg tccggttaca caagcgggcg aaatttctaa atttcctgca aataaccgtg
     3181 atattgcggt agtggttgat gaatctgttc ctgcaggtga ggtgttagag gcttgtcgta
     3241 aagctggtgg taataaatta gtggcagtaa atttatttga cgtttaccgt ggttcgaatt
     3301 tagaaagtgg taagaagagt ttagccatta gcttaacggt tcaagataat gaaaaaacat
     3361 tagaagaaaa tgaaatcaat gcggtaattc aagcggtatt agctgaatta gcaacacgtt
     3421 tcaatgcggt acttcgtgat taatcagtaa aatatggctg atttatttta tcagccataa
     3481 caataaacta aggataaatg atggcgctta ccaagattga aattgcagaa aacctgattg
     3541 aaaaatttgg gttagaaaag cgtgtagcga agcaatttgt tgaattgttc tttgaagaga
     3601 tccgatcctc cctcgaaaat ggtgaagaag tgaaattatc tggttttggt aatttttcac
     3661 tgagagaaaa aaaagctcgt ccaggaagaa atcctaaaac gggggaaaat gtggctgttt
     3721 cagcccgtcg agtagtagtt tttaaagctg ggcaaaaatt acgagaacgt gttgagcaag
     3781 ctagctccca atcatagggg cagttatatt agttagattt aatgtggagt aataaatgaa
     3841 attaaccaaa cggtggcttt ccttaggttg tttgttagct actacagcga tgttagtggc
     3901 ttgttcaagt tcagatacaa cctctaagtc tcaagtaaaa agtaaagttt atcgttcaat
     3961 ataccaatca gacctttctg atcctattat ggcaattagt cgattaagtg aacatcaaca
     4021 cgaatggaaa ggcactcgct atcgtttagg tggcaatagc aaggctggca tcgactgttc
     4081 tggttttatg caagtcactt ttaaagatct ttttgggata gatttgccga gaaccaccac
     4141 agagcaggct aaggctggtg agcgtatatc caaagacgaa ttgcgtacag gtgatttagt
     4201 ttttttcaag acagggcgtg gtccaaatgg gaaacacgtt ggtgtttatg taaaaaatgg
     4261 gcaatttctt cacgcctcaa caaagggagg ggtgatttat tctgatttgg attcgccata
     4321 ttggtctaag gctttctggc aggctcgtcg tttataattt agggtaacat acatgctgaa
     4381 aaaaatcgga caagcggtca tatttggact tttttgtgca attttaattt tgtttgtagc
     4441 tccaattatt cagagtaaag ggagttcgtt ttcattatca gcattcactc aaaaagagat
     4501 tgtaagctac aatgcagcgg ttaaaatagc ctcccccgca gtcgttaacg tatatagccg
     4561 aaattttagt tctgaaaatg aaacatttga agttaagaat ttaggttcgg gtgtgattat
     4621 gagtagcgat ggctatattt taactaacag gcatgtgatt gaagatgcgg atcaaatcgt
     4681 gattgctttg caaaacggtg ctatttcgac taatgtaaaa ttagtcggcg atgattcttt
     4741 aactgattta gctgttttaa aaattgaggg tgagaattta cctactattc cacaaaatcc
     4801 ggagcgcaaa ctacaaattg gcgatgtggt gttggcaatt ggtaatcctt tgaatttagg
     4861 acagagcatt acgcaaggga tagtcagtgc tattgggcga aaaaccttaa cagaaagtgg
     4921 tagacaaaat tttattcaga cagatgtttc cattaatcaa gggaactcag gtggtgcttt
     4981 aattaatact gctggtgagc taataggtat taacactttg cgttttggga aaaatacgaa
     5041 tgaattgtca gagggcctaa attttgcgat tcccattaat ctggcgaatc aaattatgag
     5101 taagatcatt aaagatggcc gagttattcg tggctatttt ggggtaagta cagatttgct
     5161 tgacaccgca aaaatgcaag gttatgaaaa aggggtgatc attgttaatg tagataaaaa
     5221 tgggccagcc gcaaaagcgg ggattcaagc aggtgactta gtgcttaaag tcggagatat
     5281 tgaggcattc tctccttctc aaatgatgga agagcttgct gaaatgaaac cttatactac
     5341 agtaaaagta ttggttcaac gtgatgaaca attacttaac tttgatgtaa ctattactga
     5401 gttacagact caatagggta gaaaatgaaa aaaatagtga ttacagttga tgggccgagt
     5461 ggtgcaggta aaggaacgtt atgtcatgca ttggctgaaa aattagggtt tgatttttta
     5521 gattctggag ccttataccg gattactgca ttaagcgcaa ttaaaaaaga ggttcccctt
     5581 gatgatgagg aaaaactagc ggaagttggg cgtaatcttg atattcaatt tttgccagaa
     5641 aatggcgaga ttcaggtgat tttggaagga gaaaatgtag gcgacttaat ccgaacggca
     5701 gaagcaggtc aaaatgcctc taaagtcgct gctttccctt tggtacgtga agcactttta
     5761 gaacgccaaa gagcctttag tagcgaaaaa ggtctgattg cagatggacg agatatgggg
     5821 acagttgtgt ttcctgatgc acaaattaag ctgtttttag atgcaagtgc cgaagaacgc
     5881 acaaaaagac gtgtaaaaca gttgcaagaa aagggattta atgctaactt tgacgagatt
     5941 ttagccgaga taaaagaacg tgattttcgc gatagaaatc gagcagtcgc gccacttgtt
     6001 ccggctaaag atgcgttgct gttagattca acgcatttat caattgaaga tgtgattaac
     6061 caagcattaa atcatatcgc tcaatacatt aaa
//
LOCUS       DANJWC010000139         5985 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.139, whole genome shotgun sequence.
ACCESSION   DANJWC010000139 DANJWC010000000
VERSION     DANJWC010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.139"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            211..296
                     /locus_tag="PWX02_001956"
     tRNA            211..296
                     /locus_tag="PWX02_001956"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:245..247,aa:Leu,seq:caa)
     gene            390..1214
                     /locus_tag="PWX02_001957"
     CDS             390..1214
                     /locus_tag="PWX02_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL1729938.1"
                     /translation="MKPLQNGTNKPFTITIASTKGGSAKSTNAANIGAFCAEHGLKTL
                     LIDTDTQPTLSSYYELAYQVPGGTYEFLHFKDVEPTHIISKTTIPNLDLIQSNDPSNK
                     ISPMLRDSPDGALRFSLLLQKIEGYDVIIIDTRGTRDITVDMSVLAADVLFCPILPHI
                     LSAKEFIRGTIGMYQDLETFTAFGFTLPPLKAMINCVDHTKDVKLVSDYLHTLFQNEF
                     DENKTLLEFSVPDRVAYREAATFSKPVYRQSQAEYETIRQLCALLMPQFAQSHFSE"
     gene            1226..2587
                     /gene="dnaB"
                     /locus_tag="PWX02_001958"
     CDS             1226..2587
                     /gene="dnaB"
                     /locus_tag="PWX02_001958"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL1729939.1"
                     /translation="MSNPNKPTTHLYSAEAEAAVIGGLLLDNTLFDDVIGKINSADFY
                     FGVHQALFKGITDLIEVGKPADILTLDEYLKQKNLLKEIGGFAYLAEVSKNTPSAANV
                     GAYADVVLLHSKHRQLLKLGQFIVDQTQTAKTPEKLESVIDEVEKKMTEFSLSDNTKS
                     ATDLSDIFASMLSRMELSAKNGDPVTGTPTGIQGIDEATTGGQPGDLIVIGARPSMGK
                     TAFSQTIAYHTLEKFESAPVFYHSMEMPADQILQRFLAMRARVSLQDIRQADRLDDED
                     WGKLTMAMGHILEHWKNRLVIDDEGSLTPQKLRTKVRQNARKYGKPAAIFIDYIQLMQ
                     TSQRYENRNLEIANISRSLKLLAKEMECPVYALSQLNRSLEQRANKRPINSDLRESGS
                     LEQDADVILFIYRDEIYDADSPFKGTAEIIIGKQRNGPLTSVMTNFRSEFALFENRTN
                     TTH"
     gene            2596..4245
                     /locus_tag="PWX02_001959"
     CDS             2596..4245
                     /locus_tag="PWX02_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDL1729940.1"
                     /translation="MTKDEKRLAAIAKGLNVSPISNTAPSYQTATPTHYSSDVEYITV
                     TLDKLRPYEHNPRKTRNPNFEMIKESIRRRGLDHKPNITRRPGEDFYIVADGGNTRIQ
                     ALKELFTETKDPKFWSISCQYKPWQGDTADSVEAELNLLIGHLIENDTRADLSFIEKA
                     LGILQAKEYYEKKLNKSLSARELATELEGDGYIIHYTLANKMERCVLYLYPHIPEVLF
                     KGLGHTQIDKLLAIRNNAEEVWAAHQFDTDINFESLWSENLSKFNEATPFQAKEFQSE
                     LITAMVEALDGKVSFETLYLDIDLDEQKFKKIAAKQQEIDNLVEKTVDQINTRQEQQA
                     AKFEPVIKNENKSVATEPAKNVIDDVIDDNESDDEQNNAITDLSQLDSPLLDKQDNQT
                     EDYLSQIAHNFGLTPGMSIQEQREQRAEANGLTFACEGRQPVEDIWLIHPARQYRSEA
                     YSLALDIAESVGIDHLVEHVVKQPVDYTYAMKPTDSELSPSVLTLYSFLSMLQTAKHK
                     DENNCVLDSNFLFELDDITLVKLFRLIRVSRHIHQQGGKND"
     gene            4238..4795
                     /locus_tag="PWX02_001960"
     CDS             4238..4795
                     /locus_tag="PWX02_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HDL1729941.1"
                     /translation="MIKTITVNEALIGNALLNIKEGNIHPCLKMGFSEEQLKAINNLS
                     LDEIMDISNSVVSFAKVEINHETFWKLLAIAQANTQQRQIIDRALLLGASIEMLHQYF
                     GLSTSEVSARRQLLGIEEKMGRKAAASDEESTHIWEIWQKYKQKMESLDSQEGLELLC
                     LIAEEGNMNLTVVWKLVSEWQSKHK"
     gene            4968..>5985
                     /locus_tag="PWX02_001961"
     CDS             4968..>5985
                     /locus_tag="PWX02_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729942.1"
                     /translation="MFSSINNPTEHGLLFFGNQHETVPTRLLQDPCLTPRAKFAWQLI
                     KCNVQAFQGGLFPSYETLSKMLSDRPYAEAKLSEKVVTQTLYLLRLTRWLTLCETVRD
                     ERGYVQGNVYILHDEPVPILDAIQLNSDYLSFLDKCTKHKDSLINGVANHIVESLMAD
                     KTKWHYVSHLDCLQNRYKTYQQQQQMKDKPLSQAEQELSKIQQNILTSAMEVRDFDEE
                     VRKKSSEILTSNKEVRDENDDKSLILDLLPLGKSDVLYSTLYINKYCTGEPSEITWPQ
                     EIPLTSLEQKLTAQAMKGLDLGLCQAILLEAQQRIIQKQDVRKPKGYLYTLIQRAKQG
                     EFKPY"
BASE COUNT         1944 a         1155 c         1179 g         1707 t
ORIGIN      
        1 tgcataacta tttttgtaaa gatgaattga gtgctgattt ttaaatcaac cgatttcaaa
       61 aggcattttt acgcttgcac aagcggtcat atttccctat aatcgtgcaa aaattaagcc
      121 agtgtggcga aatcggtaga cgcagcggat tcaaaatctt ttgcgtaaac tgataaaaca
      181 tacaggcaat tgaataaaaa tgcactctca gcctgagtga tgaaattggt agacatgatg
      241 gattcaaaat ccattggtga ataaccgtgt cggttcgagt ccgacctcag gcaccattca
      301 actgcgttcc tttcaaataa tactcactct aaatatacag tttataatgc caatactcct
      361 taacttcaac tttgttaaga gagtaaatta tgaaaccatt acaaaacggt acaaacaaac
      421 cttttaccat tactattgct tcgaccaaag gaggctctgc taagagtacg aatgccgcta
      481 acattggtgc attctgtgca gaacacggtc ttaaaaccct tcttatcgat actgataccc
      541 aaccgacatt aagttcctat tatgaactcg catatcaagt tccgggcgga acttatgaat
      601 ttcttcattt taaagatgta gagcctactc atatcatttc taaaacgact attcctaatc
      661 ttgacttgat tcagtcaaat gatccgtcta acaaaatcag cccaatgctg agagattctc
      721 ctgatggagc attacgtttc agtttactac tacaaaaaat agaaggctac gatgtcatca
      781 ttattgatac ccgtggaaca agagatatta ctgtcgatat gtctgtgctg gcagctgatg
      841 ttttattctg ccctattctt ccgcacattc tatctgctaa ggaatttatc cgtggcacga
      901 ttggaatgta tcaagattta gaaactttta ctgcatttgg ctttactctc ccaccgctga
      961 aagcgatgat taactgtgtt gatcatacca aggatgtcaa attagtctct gactatctgc
     1021 acacgttgtt ccaaaatgag tttgatgaaa acaaaacatt actggaattt tctgtgcctg
     1081 atcgtgtggc ataccgagaa gcggcaactt tttcaaaacc ggtctatcgc cagagccaag
     1141 ctgaatatga aaccattcgt cagctttgtg cattactgat gccacagttt gcacaatctc
     1201 atttttctga gtagttgggg gatttatgtc taacccaaat aaaccaacca cccatctcta
     1261 ttcagcagaa gctgaagccg ctgtcatcgg gggattgtta ttagataaca ctctctttga
     1321 cgatgtcatt ggcaagatta attcggctga cttttatttt ggtgtgcatc aagccttatt
     1381 caaggggatt acggatttaa ttgaggtggg gaaacctgca gatattctca cattagatga
     1441 atacctaaaa cagaaaaatc tgttgaagga aatcggcggt tttgcctatc ttgcagaagt
     1501 ttcaaaaaac accccttcgg cagccaatgt tggagcttat gctgatgtcg ttctgctaca
     1561 tagtaaacac cgacaacttt taaagctggg ccagtttatt gtcgatcaga ctcagacagc
     1621 aaaaacaccg gaaaaacttg aatcagttat tgatgaagtt gagaaaaaaa tgactgaatt
     1681 cagcctttct gacaatacca aatctgcaac agatttaagt gatatttttg ccagtatgct
     1741 ctctcgtatg gagctttcgg ctaaaaatgg tgatcctgtg actggaacac cgacaggtat
     1801 tcaaggcatc gatgaagcga ctaccggtgg gcaaccgggt gatttaattg tgattggtgc
     1861 ccgtccttcg atggggaaaa cggcattttc tcaaactatt gcctaccaca cccttgaaaa
     1921 atttgagtct gctcctgtgt tctatcacag tatggaaatg ccggcagatc aaattttaca
     1981 acgtttttta gcaatgcgtg ccagagtaag tttacaagat attcgccaag cagaccgctt
     2041 ggacgatgaa gattggggaa aactaacgat ggcaatgggg catattcttg aacattggaa
     2101 aaatcgttta gtcattgatg atgaaggtag ccttacccct caaaaattac gcaccaaagt
     2161 tcgacaaaat gctcgtaaat atggaaaacc ggccgcaatt ttcattgatt acattcaact
     2221 aatgcaaacc tcgcaacgtt atgaaaatcg caatttagag attgctaaca tctctcgttc
     2281 gctcaaatta ctggcaaaag agatggagtg cccagtgtat gcactctctc agcttaatcg
     2341 ctctttggaa caacgtgcta acaaacgtcc gatcaactct gatttacgag aatctggttc
     2401 tcttgaacaa gatgccgatg tcattttatt catctatcga gatgaaattt atgatgcgga
     2461 ttcccccttt aaaggtaccg cagaaatcat tatcggtaag caaagaaatg gaccacttac
     2521 ttctgtaatg accaatttta gaagtgaatt tgctctattt gaaaaccgaa caaacacaac
     2581 gcattaggat agattatgac aaaagatgaa aaaagactgg cagcgatagc gaaaggatta
     2641 aacgtttcgc ctatttcgaa tactgctcca tcctatcaaa cagcaacacc aacgcattat
     2701 agtagcgatg ttgaatatat tacagtgacg ctagataaat tacgtccata tgagcataac
     2761 cctcggaaaa cccgtaaccc taacttcgag atgatcaaag agtctattcg tcgtcgagga
     2821 ttagatcata agccgaatat cacccgtcgt ccgggagaag atttttacat tgtcgctgac
     2881 ggtggtaata cccgtattca ggcacttaaa gagctgttta ctgagaccaa agatccaaaa
     2941 ttttggtcca tcagttgtca atataaacct tggcaaggtg atactgccga cagtgtcgaa
     3001 gctgaactca atttacttat cgggcatttg attgaaaatg atacccgtgc ggatttgtca
     3061 tttattgaaa aagctcttgg tattttacag gcgaaagaat attacgagaa gaaattgaat
     3121 aaatcacttt cagcaaggga attagctact gagttagagg gtgatggcta tattatccac
     3181 tatacgttgg ctaataaaat ggagcgatgt gtactttatc tatatcccca tattccagaa
     3241 gttctattta aaggtttagg acatactcaa atcgataagt tattagctat tcgtaataat
     3301 gccgaagagg tatgggcagc ccatcaattt gatactgata ttaattttga atcgctctgg
     3361 tcagaaaatt tatccaaatt taatgaggct acaccatttc aagcaaaaga attccaaagt
     3421 gaattgatta cggcaatggt tgaagcttta gatggaaaag tgagttttga aacactttat
     3481 ctggatattg acttagatga acaaaaattc aaaaaaatag cggctaaaca acaagaaatt
     3541 gataatttag ttgaaaaaac agtcgatcaa atcaatacca gacaggaaca acaagcagca
     3601 aaatttgaac cagttatcaa aaatgaaaat aaatctgttg ccacagaacc agccaaaaat
     3661 gttattgatg atgtaataga tgataatgaa agtgacgatg agcagaataa cgccattact
     3721 gatttatctc aattggattc tccattattg gataagcaag ataatcaaac tgaggattat
     3781 ctctcccaaa ttgctcataa ctttggactc acaccgggaa tgagtattca agaacagcga
     3841 gagcaacgag cagaagccaa tggacttact tttgcttgtg aaggtcgcca gccggttgaa
     3901 gatatttggt tgattcatcc tgctcgtcaa tatcgttcag aggcgtattc ccttgcctta
     3961 gatattgcag agagtgtggg cattgaccac ttggttgaac atgttgtgaa acagcctgtg
     4021 gattacactt atgcaatgaa accgacagat agtgaacttt caccgtctgt tctaacactc
     4081 tattcattcc tttcaatgtt acagacagct aaacataagg atgaaaataa ctgtgtccta
     4141 gattccaact tcctgtttga gcttgatgat attaccttag tcaaactttt ccgtctcatt
     4201 cgggtcagtc gccatattca tcaacaagga ggaaaaaatg attaagacaa ttaccgtaaa
     4261 tgaagcattg attggtaacg ccctgctcaa tattaaagaa ggaaatatcc atccttgttt
     4321 aaaaatggga ttttctgagg aacaactaaa agcgatcaat aatctcagtc ttgatgaaat
     4381 tatggatatc agcaactctg ttgtgtcttt tgccaaagtg gagattaatc acgaaacctt
     4441 ttggaaattg ttggcgattg cacaagctaa tacccaacaa cgacagatta ttgaccgagc
     4501 attattgctt ggagcttcta ttgaaatgtt acatcagtat tttggtttat caacctctga
     4561 agtttctgca cgtcgccaat tacttggtat tgaagaaaag atggggcgaa aagcagccgc
     4621 aagtgatgaa gaatctaccc atatttggga gatttggcaa aaatacaaac agaaaatgga
     4681 aagcctagat tcacaagaag gcttagagct cctgtgcctg attgctgagg aaggtaatat
     4741 gaatttaacg gttgtctgga aattggtttc cgagtggcaa tcaaaacata agtaaaaaat
     4801 aaatgcccca aagttggcgc tttggggcat tcgatattgg agtcttatat ctctattcta
     4861 tggctcatag acttttagga gaccgacttt gcatgagtcc tgagtattat agccatagga
     4921 tagagaatag caagagaaaa actctttaat ccgaatggag agaaacaatg ttcagcagta
     4981 taaataaccc aacagaacac ggattgctat tttttggcaa ccaacacgaa acagtaccta
     5041 ctcgcttatt acaagatccc tgtctaacac caagggcaaa atttgcatgg cagttgatta
     5101 agtgtaatgt tcaagccttt caaggtggct tatttccttc ctatgagaca ctcagcaaaa
     5161 tgttgtctga tagaccctat gctgaggcta aattatcgga aaaagtagtg actcaaaccc
     5221 tttatttact ccgtcttacc cgatggctga cattgtgtga aaccgtacgt gatgagcgag
     5281 gttatgtgca aggtaatgtg tatattctgc atgatgagcc tgtacccatt ttagatgcta
     5341 ttcagttgaa tagcgactat ctctctttct tagataaatg caccaagcat aaagatagtc
     5401 ttattaatgg tgtagcaaat catattgtag aaagtctgat ggcagataaa actaagtggc
     5461 attatgtctc tcatttagat tgtctgcaaa atcgttataa aacatatcaa cagcaacaac
     5521 aaatgaaaga taaacctctt tcacaagctg aacaagaatt gagcaaaatt caacaaaata
     5581 tcctgacttc cgctatggaa gtcagggatt ttgatgagga agtcaggaaa aaatcctcag
     5641 aaatcctgac ttccaataag gaagttaggg atgaaaatga cgataagtcc ttgattttag
     5701 acttacttcc attagggaag tcagatgtac tgtacagtac tttatatatt aataagtact
     5761 gtactgggga acctagcgaa attacttggc cacaagaaat cccactaacc tcacttgaac
     5821 aaaaactcac tgcacaagca atgaaagggt tagatctggg gttatgccaa gctattttgc
     5881 tggaagcaca acaacgcatt attcaaaagc aggatgtgag aaaaccgaaa ggttatctct
     5941 atacgcttat ccagcgggcg aagcagggtg aatttaaacc ctact
//
LOCUS       DANJWC010000140         5962 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.140, whole genome shotgun sequence.
ACCESSION   DANJWC010000140 DANJWC010000000
VERSION     DANJWC010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.140"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(291..587)
                     /locus_tag="PWX02_001962"
     CDS             complement(291..587)
                     /locus_tag="PWX02_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729943.1"
                     /translation="MSNTIQTSNHTLSSTAECLSTAEMSLLVRLLKLARTQHELILTA
                     LPALQQLQSPLAEQAEATTQQAAILRLVEETLLARYPTIQDAELKQQIFEWFNS"
     gene            744..1202
                     /locus_tag="PWX02_001963"
     CDS             744..1202
                     /locus_tag="PWX02_001963"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729944.1"
                     /translation="MDKAFNDEYHPLATDLINDIFTVQGISNRGRIALIRQYAELLVR
                     SLLGVSFSERITLGDKKTTRALAELKAKRQHYYELAVAVEYIRDVGNAATHTLYTGEI
                     SGKMVNKSLFTWHKFMPIISLSTFTNIRLARMLPPWCCLGCCRLVFACWC"
     gene            1208..1585
                     /locus_tag="PWX02_001964"
     CDS             1208..1585
                     /locus_tag="PWX02_001964"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729945.1"
                     /translation="MHRQFPQSYAIAEKMIILLFKVHGIERMTLWVERNRRKLEQIDR
                     LAVENEEQVTQFIEKEYSAYDFALAQMGANIHPVPYQDFDVAAARYREYVSEMSVLQS
                     PEIVELKGIMDFVYLGVRQEKAR"
     gene            complement(1601..2428)
                     /locus_tag="PWX02_001965"
     CDS             complement(1601..2428)
                     /locus_tag="PWX02_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729946.1"
                     /translation="MPTEITRLTLRFDGLDATEHQVDLYSLGEALQGFARIISTAGHF
                     SVTQTYSKSFSMHSVKTYAQEVRANCFSVDMVLNWAQQNQILSGSFGILLAAVIPYIF
                     QRNKAKTEEMKMLKDSLDKAIEALGNRDQAVIERLASTIEKMAEELRPAARLAVSPIG
                     ESCKTISIIDTQTKQSIGIINESDKESIMSLSENEITGIQVFDILISELDKVKATCKV
                     NINNAEQRTNAVISDPLFGSAENPYLQAFAQNKAIKVTGKATIKDGEIIKLTIMDIH"
     gene            complement(2445..3284)
                     /locus_tag="PWX02_001966"
     CDS             complement(2445..3284)
                     /locus_tag="PWX02_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1729947.1"
                     /translation="MKWYEFAEQQRKICGLTQVELGDKLNVEQGTVGNWLNGRREPEL
                     ATILKIFHALGITQVTFNSDSQIVDIQSKQRIHWYELAEQRRKNLGLSQAELADEFEV
                     SQATVGNWLSNRREADIYTIAKLLERLGIVDVVLHTDATISLTSGIFDTNTPQLKVSA
                     ALKFRGQDIGEKLTLEDELSLHYFSQDVRAYAVRIDGEKLEPRIVSGEYIVVEPGAKL
                     QSYDEVLIKLKDGRYMIRVLLTGRNKEWRYADPNTMQQDTDFDPSQIETMEYIAAIIK
                     PPR"
     gene            complement(3284..3943)
                     /locus_tag="PWX02_001967"
     CDS             complement(3284..3943)
                     /locus_tag="PWX02_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729948.1"
                     /translation="MKEKWNDIVKRRIKELDIHRTVFAERVGVSSSGLGSWLNESRVP
                     NLDQIAKMLYLVGIDSVTLHSADCLMSVRESYKLNKIEGVPVKGSLAFGFPEMGNAVE
                     KATLKTAEQKQFIDFRASSPRSEAYQILGTALSPRIRRNEFVVIDPNKKPLETDEVLV
                     YFKETEEYAIKRLVELAKKEGDPVFLADLNQDMLRMAVEEDAITMYRIEGFAKESKVI
                     R"
     gene            4040..4234
                     /locus_tag="PWX02_001968"
     CDS             4040..4234
                     /locus_tag="PWX02_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1729949.1"
                     /translation="MNKIRQFRRAIGLSQQELASVIGVTTGAIGFYERGLREPNLATI
                     KKLAEALNCKPADLYPVLEG"
     gene            4283..4726
                     /locus_tag="PWX02_001969"
     CDS             4283..4726
                     /locus_tag="PWX02_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017806454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729950.1"
                     /translation="MTMKQTIIEMIESCTGGKSAVAGFLGFTESELNNRLYQTKGQRF
                     KDEELIAIEQEFGVSHWSDEINRQLGKVAFCVPNADELDAEELSELQLHELAKRGLLF
                     AELEKALADGEVTQAEAEVLSKLLNRSQCATAKVITATIELYSKR"
     gene            4789..5628
                     /locus_tag="PWX02_001970"
     CDS             4789..5628
                     /locus_tag="PWX02_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HDL1729951.1"
                     /translation="MNQLLNISEQKSSITMSSREIAELCEKEHRHVLRDIRAYVGAII
                     QMERGINVKSLDWSGKEGVELFGHTPIGGVICRYEVNPQNNQSYPIYYLDKSATLTII
                     SGYNILLRKKIIDRWQELETQVPQSSKTPHIPQTYAEALRLAADQAEQNQLLQLENKQ
                     KTEENNALKSYFEPGLTPAQFVKGLNGVNSNKINDYLRTRGWLYKEKHCWRVCSQVRD
                     KYLTESFKRIEVDPVERIEMTTYKPILLEKGAVKIFEAYLQGKLPMKADWNGKYYHSK
                     VAV"
     gene            5625..>5962
                     /locus_tag="PWX02_001971"
     CDS             5625..>5962
                     /locus_tag="PWX02_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729952.1"
                     /translation="MRFTSTINNVRLVEWEINITQGALVDLINQASSWAKAVVIDGIT
                     YYWMSFSKVCEELPAVFSKEDTVYRQYKVLKEKGIIDHFKMDGKDYVRLTEKGCEWNK
                     FEPIRESEKNP"
BASE COUNT         1692 a         1149 c         1334 g         1787 t
ORIGIN      
        1 gggtatatgc ttgtattcct ttgcttgcac caaagggcat aacgctttga agtagtgggc
       61 gttttaaggg ctgcctccga ctttcgtctt cctacttttc accattaggc ttaaagcggt
      121 tttgaatttg atttttaaag aacattgagc cgtagctcgt tttgatggga gaataataca
      181 ttatgtgtca aattaagatc aatacaaaaa gagtgaaaaa ttatacaaaa tgtgttgatt
      241 ggctattttt taatctatta agggtaaaaa gccccttccg gggcttgtga ttatgaattg
      301 aaccattcaa agatttgttg cttgagttca gcgtcttgaa ttgtggggta gcgagcaagt
      361 agggtttctt caacaaggcg gaggattgcc gcttgttgag tggttgcctc ggcttgttct
      421 gctaatgggc tttgcagttg ttgcaatgcc ggtagggctg ttaaaataag ctcgtgttgt
      481 gtgcgagcta attttaacag gcgaaccaag aggctcattt ctgcggtgga taagcattcg
      541 gcagttgagc taagtgtgtg atttgatgtt tgaatagtat ttgacatagc gtcctccgtt
      601 gatctaaaaa caaattgccc tgaacattca gcgacattgt gtgaagtgag gcttttttag
      661 atcggtacgc tgtcgatatg atgttaaaat aatgcaaatg aaggttattg tctgtgatca
      721 atttcatatt ttgaggataa aaaatggata aagcatttaa cgatgaatat caccctttag
      781 cgaccgattt gattaatgat attttcacgg tgcaagggat ttcaaatcgg ggcagaatag
      841 cccttattcg ccagtatgcg gagctgttgg tgcggtcttt actcggcgtg tcattttcag
      901 agcgtattac gctgggcgat aagaaaacca ctcgtgcgtt ggcggagtta aaagccaaaa
      961 ggcagcacta ttacgagctg gcggtagcgg tggaatatat tcgagatgtg gggaatgccg
     1021 caacgcatac actctacacc ggtgagattt cggggaagat ggtaaataaa tcactattca
     1081 cttggcacaa atttatgcct attatttcgt tgagtacttt cacaaatatc cgcttggctc
     1141 gaatgctgcc gccttggtgt tgtttgggtt gttgccgcct cgttttcgct tgttggtgtt
     1201 gatccgattg catcgtcaat ttcctcaaag ttatgccatt gcggaaaaga tgattatttt
     1261 attgtttaag gtgcatggca ttgagcgaat gacattatgg gtggaacgaa accggcgtaa
     1321 gctggagcaa atagatcggt tggcggtaga aaatgaagaa caggtgacgc agtttatcga
     1381 aaaagagtac tccgcttatg attttgccct tgctcaaatg ggggcgaaca ttcaccctgt
     1441 accatatcag gattttgacg tggcggcagc tcgttatcgt gaatatgtat cggaaatgag
     1501 cgtgctgcaa tcgccggaga ttgtagagct gaaagggata atggattttg tgtatttggg
     1561 ggtgaggcaa gaaaaagccc gctgatggcg ggcagaaaat ttagtgaata tccataatcg
     1621 tcagtttgat gatttcgccg tcttttatgg ttgctttgcc ggttactttg attgctttgt
     1681 tttgagcaaa ggcttgtaaa taaggattct ctgcactgcc aaatagcgga tctgaaatca
     1741 ccgcatttgt gcgttgctca gcgttattga tgtttacttt acaagttgct ttaactttat
     1801 ccagctctga aatcagtata tcaaatacct gaatcccggt tatttcattt tcagacaagg
     1861 acataatcga ttctttgtct gactcattaa ttatgcctat agactgcttg gtttgtgtat
     1921 caataatgga gattgtttta caactttccc cgattggcga aacagcaagt cttgctgcag
     1981 ggcgaagctc ttccgccatt ttttcaatgg tggaagccag tctttcaata acagcttgat
     2041 ctcgattacc caaagcctct atagccttgt ctaacgagtc tttcaacatt ttcatttctt
     2101 ctgtttttgc cttgtttcgc tggaaaatat aagggataac cgctgcaagc agtatgccaa
     2161 atgaaccgga taatatctga ttctgttgag cccagtttaa taccatatca accgaaaagc
     2221 aattcgctct gacttcttgt gcataagttt tcacgctatg catagaaaaa cttttagagt
     2281 aggtttgagt aactgaaaaa tgaccagcag ttgaaatgat gcgagcaaag ccttgtaagg
     2341 cttcgccgag gctgtaaaga tcaacctgat gctctgttgc atccaatccg tcaaatctaa
     2401 gggttaatcg ggttatttct gttggcattg ttactcctta ttaattatct cggcggctta
     2461 ataatggcgg cgatgtattc cattgtctca atttggcttg ggtcaaaatc ggtatcttgt
     2521 tgcattgtat taggatcagc atatcgccat tctttatttc tgccggtcag taatacccta
     2581 atcatatatc tgccgtcttt gagtttaatc aacacttcat catagctttg gagcttcgct
     2641 cccggctcta ccacgatata ttcgccactc acaatgcgag gctcgagttt ttcgccatca
     2701 atccgtactg cataggcacg cacatcttga gagaagtaat gtagtgacaa ttcgtcttct
     2761 agcgttaatt tctccccgat gtcctgcccc ctaaatttta aggcggcact gactttgagt
     2821 tgtggtgtat ttgtatcaaa aatacctgag gtcaaagaaa ttgtggcgtc ggtatgtaat
     2881 accacatcga caatccctaa tcgctccaat aatttagcaa tagtgtaaat atccgcttct
     2941 ctacggttac taagccaatt cccaaccgtt gcttgagaaa cctcaaattc atctgccaac
     3001 tcagcttgtg aaagccctag gtttttacgt cgctgttcag ctaattcata ccagtgtatt
     3061 ctttgtttac tttgtatatc gactatttgg ctatctgaat taaaggtaac ttgagttatg
     3121 cctaatgcgt ggaaaatttt taatatggtt gctagttcag gctcacgtcg tccatttaac
     3181 caatttccaa cagtgccttg ctcaacgttt aatttatccc ccagctctac ttgggtaagc
     3241 ccgcatattt tcctctgctg ctctgcgaac tcataccatt tcattaccga atcaccttac
     3301 tctctttcgc aaagccttcg attcgataca ttgtgattgc atcttcctca acagccatac
     3361 gcaacatatc ttgattcagg tcggctaaga aaacggggtc gccctctttt ttcgctagtt
     3421 cgactaaacg tttgattgca tattcttccg tttctttgaa atacaccagc acttcgtctg
     3481 tttcgagtgg ttttttattt ggatcgatca ccacaaattc attgcggcga atacgaggac
     3541 tgagagccgt gcctaaaatt tgataagcct ctgaacgagg ggaagaggct ctaaaatcaa
     3601 taaattgttt ttgctcagcg gttttgagtg tcgccttttc tacggcattg cccatttcgg
     3661 ggaagccgaa agcgagcgag ccttttaccg gcacaccttc aattttattg agtttatagc
     3721 tttctcttac agacattaaa cagtctgcac tatgtagtgt tacgctatca atgcctacta
     3781 agtaaagcat tttggctatt tggtcgaggt ttggcactct actttcattt aaccaacttc
     3841 ctaaaccaga agatgaaact cccactcgct ctgcaaaaac agttcgatga atatccagct
     3901 ctttaattcg acgtttaaca atgtcattcc atttctcttt cataggtgca ttttacctca
     3961 taaaaccagt aaataagtta tctcctcttt acaataaata acactaaatg tattattgca
     4021 cattaagagg ttttaattta tgaataaaat tagacaattc cggcgggcta tagggttatc
     4081 tcaacaagaa ttagcgtctg taattggtgt taccactggt gctattggat tctacgaaag
     4141 aggtttacga gaacccaacc tagccacgat taaaaaactt gctgaagcct taaactgcaa
     4201 gccagcggat ttatacccgg tgcttgaagg ttagtttagg cttttggctg aaaatgaaaa
     4261 ccacaaaaaa cgataggaaa ttatgacgat gaaacagacg attatcgaga tgatcgagag
     4321 ttgcaccggt ggcaaatcgg ctgtagccgg ctttcttgga tttactgaga gcgagctaaa
     4381 caaccgctta taccaaacca aaggacaacg ctttaaggac gaagagctga ttgcgattga
     4441 gcaggaattt ggcgtaagcc attggagtga tgaaattaat cgtcagcttg gcaaggtggc
     4501 gttttgtgtg ccgaatgcag acgagctaga tgcggaggaa ctttctgaac tgcagttaca
     4561 cgagcttgcg aaacgaggct tgttatttgc agagctggaa aaggcattgg cggacggcga
     4621 ggttactcaa gccgaggctg aggtattaag taagctatta aaccgttcgc aatgtgcaac
     4681 ggcaaaggtg attacagcaa ccattgagct gtatagcaaa cgttaggcaa taaaaaacca
     4741 ccgctgtaac ggtggctctt taaaactaaa caacgaaagg tattttcgat gaatcaatta
     4801 ttaaacattt cagaacagaa atcaagcatt acgatgagta gtcgtgagat agcagagtta
     4861 tgcgaaaaag aacatcgtca cgttttaaga gacattaggg cttatgttgg tgctattatc
     4921 caaatggaga gaggaattaa tgttaaatct cttgattgga gcggaaaaga gggtgtagaa
     4981 ctttttggac atacccctat tggcggagtt atatgtcgat atgaggttaa tcctcaaaat
     5041 aatcaaagtt acccaatcta ttacctagat aaatcagcaa cgctgacaat tatctctggc
     5101 tacaatattt tgcttagaaa gaaaattatc gaccgctggc aagagctgga aacgcaagtg
     5161 ccgcaatctt ctaaaacacc tcatattcca caaacgtatg cggaagcgtt acgtttggcg
     5221 gcagaccaag cagaacaaaa tcagttattg cagttagaga acaagcaaaa gaccgaagag
     5281 aacaatgcac tgaaatcgta ctttgaacct ggtttaacac cagcccaatt tgtgaaaggg
     5341 ctgaatggcg taaacagcaa caaaattaat gattatttgc gaactcgtgg ctggttatac
     5401 aaagaaaagc attgttggcg agtgtgtagc caagttcggg ataagtatct aacggaatca
     5461 tttaagcgaa ttgaggttga tcctgtagag cgtattgaga tgactaccta caagccaatt
     5521 ttgcttgaga aaggtgcggt taagattttt gaggcttatt tacaaggtaa gttaccaatg
     5581 aaagctgatt ggaacggcaa atattatcac agtaaggtgg cggtatgaga ttcacatcaa
     5641 caataaacaa cgtccgctta gtggagtggg aaatcaacat cacgcagggg gcattggtcg
     5701 atttaatcaa tcaagcctcc agttgggcaa aagcggtggt tattgacggc ataacctatt
     5761 actggatgtc gtttagcaaa gtgtgcgaag agttacctgc tgtatttagc aaagaagata
     5821 cggtttaccg ccaatataag gtgttgaaag aaaaaggcat tatcgaccac ttcaaaatgg
     5881 acggtaaaga ttatgtccgt ttaactgaaa aaggttgtga atggaataaa ttcgagccga
     5941 tccgagagtc ggaaaaaaat cc
//
LOCUS       DANJWC010000141         5944 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.141, whole genome shotgun sequence.
ACCESSION   DANJWC010000141 DANJWC010000000
VERSION     DANJWC010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5944
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.141"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(116..745)
                     /gene="hflD"
                     /locus_tag="PWX02_001972"
     CDS             complement(116..745)
                     /gene="hflD"
                     /locus_tag="PWX02_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HDL1729953.1"
                     /translation="MADYHDITLAYAGVCQAATLVQQFAHKGMADREAFKCALESLLI
                     TQPESTLSVFGDDLSHLKMGLETALAQTGGGNGKLDTEVGRYWISLLALSQKLNKNPE
                     AKQQLAQRLQQVERQLALYEDNMMADQMIANLAAIYSDIISPLGTKIHVMGMQDYLVR
                     PDIQNKIRASLLAGIRAGILWQQVGGSRWQFLFSRKKIFNQAQKFYRQI"
     gene            complement(853..2541)
                     /gene="cydC"
                     /locus_tag="PWX02_001973"
     CDS             complement(853..2541)
                     /gene="cydC"
                     /locus_tag="PWX02_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HDL1729954.1"
                     /translation="MKSLFPFFALYQTHFGRLLLGVVLAILGLAASIGLLSLSGWFLA
                     ASFLAGNALIFNFFYPSSGVRGLAVGRTISRYFERLVTHDATFRVLANLRASVFKKLI
                     PLSPSGLNRFRNSELLNRLVADVDTLDTLYLNLVSPFVSAVMIIAFMAIGLSFVSVPL
                     MLIICGTLLVLLAVIPTVFYRLGLKLGRNAIQSRANYRSQFIEWVQLNAEFLLFGNLN
                     QMSEKLQQTEHQWLNAQSKESRLSGLSNSLIMLSNGILTCVVIYLVSTSINVPTAKYP
                     EALIALVIFCVMASAEILSPIGIAFLHLGQVITAAERISEITEQQPNVTFGSKAEWQN
                     LSQNQPLVRFENVSFAYYGEQNVLNNLSFEVLKGQKVAILGKTGSGKSTIFQLLNRNY
                     DPTSGQIWLNNCKIDEYSEPMLRSKLVTLSQRVHIFSQTLKDNLLMGNAQATDEQMQE
                     VVRKVGLGHLLEIDGLNLWLGEGGRPLSGGEQRRLGLARLLLSSAELVLLDEPTEGLD
                     RETEQQIINLILTHCCDRTLIMITHRQSGLDKFDVVYRIDNGRLVGWFLQQIAY"
     gene            complement(2541..4307)
                     /gene="cydD"
                     /locus_tag="PWX02_001974"
     CDS             complement(2541..4307)
                     /gene="cydD"
                     /locus_tag="PWX02_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HDL1729955.1"
                     /translation="MEKQRQRELQKWLKSQQKVIKKYMHLNILLGSFSSLCMIGQMWL
                     IATMLDKMLIGKQSPNSFLVEIMLLFCCFAARAFFIFLRERVGFKAGQTLRLHLRRQI
                     LAKMEAVGPMSIQQKPAGSWATLMLEQVENLHNFYARYLPQQFLSLIVPLLILCFVFP
                     INWAAGAILFATMPLLPLFMILAGMKAVEANQRNIGILSRISGQFLDKLKGLETIRLF
                     GQAEKQTEQIYQSTEDFRISTMDVLKMAFLSSAVLEFFTAVSIAVTAVYFGFIFLGEL
                     DFGYYGTGVTLFIGFFCLMMAPEFYQPMRELGVFYHDKAAAISAADNIETFLKEKVKT
                     QGGNLQKNVENQPLVIQAKDCVIFSPQGKALTQALNFELNAHQHIALVGQSGAGKSSL
                     MNMLLGFLPYEGSVTINGTELRDLNLSQWRAKLAWVGQNPQLMHGSLKENILIGNPNA
                     TDSELAKALQLSKADEFVARLGLEHQVQDSNIGISGGQAQRIAIARALLRPYELLLLD
                     EPTASLDMDSEQQVLAALHNLSREQTTLMITHRVEDLTQCDEIWVMKQGQIIQKGRFT
                     ELEHTGFFAELLHNELVHQGEI"
     gene            complement(4422..>5944)
                     /locus_tag="PWX02_001975"
     CDS             complement(4422..>5944)
                     /locus_tag="PWX02_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HDL1729956.1"
                     /translation="VNLDLTSNAIKFIQKMEGGKSFPLTVSPLKIDLKMSDNNLTLKS
                     DLRVENNGRLSTDLVMKDLSKARALSGNIHLDQLSLKLIRPLLERGDSVDGNVNARLT
                     VSGTATSPLLHGNLNVTELKARSVTMPFDITGGNLAMNFHGATSTLSGKLQTKQSELR
                     LEGDADWRDLNAWKTRVHAQANRFQVDIPNIAKVAVSPDIQVTATPTLLTLSGNVDIP
                     WARIEVEQLPESAVSVSGDEVIMDGSVKNKVPFSQRNIPAQTSGGMAINADIKINIGN
                     DVKLKAYGLNSNVNGLLSVRQGKQGLGLYGQVHLREGRFAAYGQDLLIRKGDISFAGS
                     PSQPTLDIEAIRNPEAMEDPTITAGVRVTGLADNPTVKVFSDPAMSQNEALSYILTGR
                     SLESSGDTSSSNAVAAALLSMSLSKSSKLVGDVGSTFGLKDLSVTTAGIGDNTKVEVS
                     ASLAPKFRVKYGVGIFAPLTELTLRYNLTPRLYLQWVSSINQAVDLMYRFEFDELF"
BASE COUNT         1679 a         1352 c         1154 g         1759 t
ORIGIN      
        1 taaaaatcgg acgaaggctc gcaactttgt cttgataacg tccatcaatc ggggataacg
       61 cagttaaagc ggtaagttcc attgttttat attctccaaa taggtaaatg ttcggttaga
      121 tttgacgata aaatttctgt gcttggttaa aaattttctt gcgtgaaaat aaaaattgcc
      181 aacggcttcc gccgacttgc tgccacaaaa taccggctcg aataccggct agtagcgagg
      241 ctcgaatttt attctgtata tccgggcgaa ccaagtaatc ttgcattccc atcacgtgaa
      301 ttttcgtacc taacgggctg ataatatcgc tataaatagc ggctaaattg gcaatcattt
      361 gatctgccat catattatct tcatacaggg ctaactggcg ttccacttgc tgtaaacgct
      421 gggctaactg ttgttttgct tcagggtttt tattgagttt ttggctaagg gcgagcaggc
      481 ttatccaata acgtccgact tcggtatcta atttgccgtt accgccgcca gtttgagcta
      541 atgcggtttc taagcccatt tttaaatggg aaagatcatc gccaaataca cttaacgttg
      601 attccggctg agtgattaac aggctttcta aagcacactt aaaagcctct ctgtctgcca
      661 tgcctttatg tgcaaattgt tgcactaacg tcgccgcttg gcaaacgccg gcataagcaa
      721 gggtaatatc gtggtaatcc gccatctctg taaaattcaa tatgataaaa cattagtggg
      781 aatatagcat tttttcagct tcttttgaat ggcaaaaggc atttattcta ttgaaaaata
      841 aatgcctttt cattaatacg caatttgctg caaaaaccat cctaccaatc gcccattatc
      901 tattcgataa accacatcaa atttatccag tccgctttgg cggtgagtga tcataattaa
      961 ggtgcgatca cagcagtgag tcaaaattag gtttataatc tgctgttcgg tttctctgtc
     1021 cagtccttct gttggctcat caagtaagac taattcagca gaactcagga gcaaacgggc
     1081 aagccctaaa cggcgttgtt cgccaccgga aagcggtctg ccgccctcgc ctaaccataa
     1141 attcaagccg tctatttcaa gcaaatgccc taagcctact tttcttacta cttcttgcat
     1201 ttgctcatct gtagcttgag cattccccat taataaatta tcttttaggg tttggctaaa
     1261 aatatgcact cgttggctta aggtaactaa ttttgagcgt aacatcggct ctgaatattc
     1321 gtcaatttta caattattca gccaaatttg accgcttgta gggtcgtagt ttcggtttaa
     1381 cagttggaat atggtcgatt ttccgctccc tgttttacct aagattgcca ctttctgccc
     1441 ttttaatacc tcaaaggaaa gattatttag tacgttttgc tcgccgtaat aagcaaagct
     1501 cacgttttca aagcgtacaa gcggttgatt ttggctcaaa ttttgccatt ctgccttgct
     1561 accaaatgtt acattaggct gttgttcggt aatttcacta atccgctcgg cggcggtaat
     1621 cacttgccct aaatgcaaaa aggcgatacc aatcggggaa agaatctccg ccgatgccat
     1681 tacacagaaa atcactaacg caattaaggc ttcagggtat tttgcagtgg gcacattaat
     1741 cgaagttgaa accaaataga tcaccacaca agtcaaaatg ccgttggata acattatcaa
     1801 gctatttgat aagccggaca gtcggctttc tttgctttga gcgttcagcc attggtgttc
     1861 agtttgctgt agtttttcgc tcatttggtt caagttgcca aagagtaaaa attctgcatt
     1921 taactgtacc cactcaataa attggctgcg gtaatttgct cggctttgta ttgcattacg
     1981 ccctaatttc aaacccaatc gataaaatac ggtaggaata acggcaagca ataccaatag
     2041 tgtgccgcaa atgatcagca ttaatggcac ggaaacaaaa gaaagcccga ttgccataaa
     2101 tgcaataatc atcactgcac tgacaaaagg cgagactaaa ttcagatata gggtgtctaa
     2161 cgtatccaca tctgccacca agcggtttaa caattcgctg tttctaaagc gatttagccc
     2221 gcttggactc agtggaatca attttttaaa gacgctagca cgcaaattcg ccaatacccg
     2281 aaaagtcgca tcgtgggtga ctaaacgttc aaagtaacgt gaaattgtcc gccctactgc
     2341 caaccctctc acccccgatg aagggtagaa aaagttaaaa ataagcgcat tgccggctag
     2401 aaatgaggca gctaaaaacc aaccggaaag gctgagcaaa ccgatacttg ccgctaatcc
     2461 taaaattgcc agcacaacac ctaataatag tcgcccaaaa tgggtttggt agagagcaaa
     2521 aaacgggaac aacgatttca ttagatttct ccctgatgca ctaattcatt atgtaataat
     2581 tccgcaaaaa agccggtgtg ttctaattca gtgaaacgac ctttctgaat gatttgccct
     2641 tgtttcatca cccaaatttc atcgcattgg gttaaatctt ccactcgatg agtaatcatt
     2701 agcgttgttt gctctcggct gagattatgc agagcggcta acacttgctg ttccgaatcc
     2761 atatcaaggc ttgcagtcgg ctcgtctaac agtaataatt cgtacgggcg aagcaacgct
     2821 cgtgcaatcg caatccgctg tgcttgcccg ccagagatac cgatattact gtcttgcact
     2881 tgatgctcca accctaaacg tgccacaaat tcatcggctt ttgaaagctg taatgccttg
     2941 gctaattcag aatctgtcgc attagggttg cctattaaaa tattctcttt caaactgcca
     3001 tgcattaatt gcggattttg ccccacccac gctaatttcg ctcgccattg gcttaaattt
     3061 aaatctcgca gttcggtgcc gttaatcgta accgaccctt cataaggcaa gaaaccaagt
     3121 aacatattca ttaatgaact ttttcctgca ccactttgcc caaccaaagc gatatgttgg
     3181 tgtgcattca actcgaaatt taacgcttgg gttaaggctt tgccttgtgg tgagaagatc
     3241 acgcaatctt tggcttgaat cacaagcggt tgattttcaa catttttttg taaattgcca
     3301 ccttgcgttt tcaccttttc ttttaagaaa gtttcgatat tatctgcggc actaatcgct
     3361 gctgccttgt cgtgataaaa cacgccaagt tcacgcatcg gttggtaaaa ctcaggtgcc
     3421 atcattagac aaaagaagcc gataaataag gtcacgcccg taccataata gccaaaatcc
     3481 agctcaccta aaaaaataaa gccaaaatag accgccgtca ccgcaatcga taccgccgtg
     3541 aaaaactcca ataccgcaga ggataaaaac gccattttta gtacgtccat cgtactgatt
     3601 cggaaatctt cggtgctttg gtagatttgt tcagtctgct tttccgcttg cccaaataag
     3661 cgaatggttt ctaaaccttt taatttatct aagaactgtc cgctaatacg ggaaagaatg
     3721 ccgatattac gctgatttgc ctccactgct ttcattccgg ctaaaatcat aaataaaggt
     3781 aataatggca ttgttgcaaa taaaatagcc cctgctgccc agttaatcgg gaatacaaaa
     3841 cagaggatca gtaatggcac tatcagcgat aaaaattgct gtggcaaata gcgtgcgtag
     3901 aaattatgta gattttccac ctgctcgagc attaaggttg cccagctgcc cgccggtttt
     3961 tgttgaatgc tcatcggccc gactgcttcc attttggcta gaatttggcg gcgaagatgt
     4021 aaacgcaagg tttgccctgc tttgaaaccg acacgctcac gcagaaaaat gaagaatgct
     4081 ctggcagcaa aacaacagaa taagagcata atttcaacca gaaagctatt cggtgactgc
     4141 tttccgataa gcattttgtc taacattgtg gcaatcagcc acatctgtcc aatcatacat
     4201 aaactactga aactccctaa cagtatgttt aagtgcatat attttttgat gactttttgc
     4261 tgacttttaa gccatttttg taattcgcgt tggcgttgtt tttccataat tttgtcaatt
     4321 tttttgtttt ttcaaccgct tgtaaatcgg gtttgccatt ctactataaa gcaaaaaggt
     4381 tgttaatggc gaaacattaa caacctttca ttcagcctta actagaatag ctcgtcaaat
     4441 tcaaagcgat acattaaatc taccgcttgg ttaatacttg agacccattg taagtacaat
     4501 cttggcgtta aattatagcg taaggttaat tcggtaagcg gtgcaaaaat gcccacgcca
     4561 tacttcactc tgaatttagg ggcaaggctg gcacttactt ctactttggt gttatcgcca
     4621 atacctgcgg tggtcacgct taaatctttg agtccgaaag tgctaccaac atcacccacc
     4681 aatttactgc tttttgataa gctcatactg agtaatgccg ctgcgactgc attactggag
     4741 ctggtatcgc cactgctttc caatgaacga ccagttaaaa tataggaaag tgcttcattt
     4801 tgcgacattg ccggatcaga gaatactttt accgttggat tgtctgccaa accggtcact
     4861 cttaccccag ctgtaatggt cggatcttcc attgcctccg gattgcgtat agcttcaata
     4921 tctaaggttg gctgagacgg tgagccggca aaactaatat cacctttacg aatcagcaag
     4981 tcttgaccat aagcggcaaa tcgtccttca cgcaaatgaa cttgcccgta aagccctaaa
     5041 ccttgtttgc cttgtctgac agacaataag ccattcacgt tactgtttaa accataggct
     5101 ttgagtttga catcattacc gatgttaatc ttaatatctg cattaattgc cattccacct
     5161 gaggtttgag ccggaatatt acgttggcta aacggcactt tatttttcac agagccgtcc
     5221 ataatcacct catctccact tacactgacc gcactttcag gtaactgctc gacttcaatt
     5281 cttgcccaag ggatatctac atttccactt aaggttaaaa gagttggcgt tgccgtcact
     5341 tgaatatccg ggctaactgc cacttttgcg atattaggaa tatccacttg gaaacggtta
     5401 gcttgagcgt gaacccgtgt tttccacgca tttagatctc gccagtctgc atcaccctcc
     5461 aaacgcagtt cgctttgttt ggtttgtaat ttaccgctta aagtcgatgt tgccccgtgg
     5521 aaattcattg ctaaattccc acctgtaata tcaaacggca ttgtaacaga gcgagctttt
     5581 aactctgtaa cgtttagatt tccgtgcaat aacggagagg ttgcggtgcc gctcacggta
     5641 agacgagcat tcacattgcc atcaaccgaa tcgccccgtt caagtagcgg tcgaatcagc
     5701 tttaagctta attggtcaag atgaatattg ccggataacg cacgagcctt acttaaatct
     5761 ttcatcacta aatcagtcga aagtctgccg ttattttcga cccttaaatc agatttaagg
     5821 gttaaattat tgtcagacat tttcagatca attttaagcg gtgaaacggt gagtgggaaa
     5881 ctttttcccc cctccatttt ttgaataaat ttaatcgcat tagaggttaa atccagattc
     5941 actt
//
LOCUS       DANJWC010000142         5750 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.142, whole genome shotgun sequence.
ACCESSION   DANJWC010000142 DANJWC010000000
VERSION     DANJWC010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5750
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.142"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            2..712
                     /locus_tag="PWX02_001976"
     CDS             2..712
                     /locus_tag="PWX02_001976"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005819058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HDL1729957.1"
                     /translation="MVALAAIFISIFGTIYALLRLRHPSSVGVVARWFGALHKLVGLE
                     LITRKKPEINYPVVYIGNHQNNYDMVTISSMVAEKTVSIGKKSLIWIPFFGLVYWATG
                     NIFIHREKRSSAIETMNKAGQIVKERNVSVWMFPEGTRSRGRGLLPFKTGAFHLAISA
                     GVPIVPVVCSNLHNKVDLNRSNNGTVICEMLDPIDTSGYSRENVKELMEKCHQIMLAK
                     FEQLNQEVEQLENTAKNA"
     gene            714..2138
                     /locus_tag="PWX02_001977"
     CDS             714..2138
                     /locus_tag="PWX02_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HDL1729958.1"
                     /translation="MKKQITRRQFLQGSALAAGFATVPTPLLAAERPQLNIPPLMDIG
                     RGKPVRLDLRPAQTQFDKGKLVDVWGVNGQYLAPTVRVKSGDFVKLTYLNNLPQKISM
                     NIQGLQASSEMIGTVHRSLDPKSSWSPIVSINQPACTCWYHADTMLNSAFQVYRGLAG
                     LWMIEDEQSKKATLPNKYGVNDIPLILQDQLLNKDGVQVLDKNTSQFFGKRLFVNGQE
                     SPYFNVPRGWVRLRIVNASLSRAYELRLDNEQPLYLIATGLGMLAEPLETESVQLAPS
                     ERVEVLVDLSEGKAVSLISGQKRDIFYKIGQVFADNDELNDNVILELRPEGMAAVLNN
                     KPSLPAFNLEEFNLKISQERRFAIRPADYLINQKRFDPKRIDFTAQKGSIERWYLHST
                     QAVGFTLQGAKFIIETLNRKQYPIKQLAWRDTVWLEAEQEITLLVRFDNTAPEQLPFT
                     FGVSDFMLRDRGTMGQFSVIENGQ"
     gene            2192..3145
                     /gene="accA"
                     /locus_tag="PWX02_001978"
     CDS             2192..3145
                     /gene="accA"
                     /locus_tag="PWX02_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDL1729959.1"
                     /translation="MSQEFLDFELPIAELEAKIESLRAVSEQDDKINLDDEIKRLQKK
                     SEELTKKTFANLDAWQISRMARHPNRPYTLDYIEHIFTEFDELAGDRAFADDKAIVGG
                     IARLDGRPVMVIGHQKGRTVKEKVKRNFGMPAPEGYRKALRLMEMAERFNMPIITFID
                     TPGAYPGIGAEERGQSEAIARNLREMSQLSVPVICTVIGEGGSGGALAIGVGDKVNML
                     QYSTYSVISPEGCASILWKSADKASTAAEVMGLTAPRLKELELIDNIVPEPLGGAHRN
                     VEQIAQNLKQRIVEDLAGLDQLNKEDLLERRYQRLMNYGYV"
     gene            complement(3240..3315)
                     /locus_tag="PWX02_001979"
     tRNA            complement(3240..3315)
                     /locus_tag="PWX02_001979"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3280..3282),aa:Thr,seq:tgt)
     gene            3434..4294
                     /gene="pdxY"
                     /locus_tag="PWX02_001980"
     CDS             3434..4294
                     /gene="pdxY"
                     /locus_tag="PWX02_001980"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HDL1729960.1"
                     /translation="MKNILSIQSHVVYGYAGNKASTFPMQLLGVDVWALNTVQFSNHT
                     QYGKWGGMVMPKEQIGSIVDGIHNIGELHRCDAVLSGYIGSAEQVAEIIQAFHKVKAE
                     NPNAIYVCDPVMGHPDKGCIVADGVKEGLINLAMAQADIITPNLVELRELTGLTVENF
                     EQAVEAVKVILTKGPKKVLVKHLSRVGKESNKFEMLFANQDGIWHISRPLYQFAKEPV
                     GVGDLTAGLFVANLLNGKSDLEAFEHTANAVNEVMEQTARSGLYELQIIASRDRIVNP
                     QCSYQAVKIA"
     gene            complement(4381..5750)
                     /locus_tag="PWX02_001981"
                     /pseudo
     CDS             complement(4381..5750)
                     /locus_tag="PWX02_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992286.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
BASE COUNT         1693 a         1196 c         1251 g         1610 t
ORIGIN      
        1 aattgttgcc cttgccgcta ttttcatttc tatttttggc actatttatg cgttactgcg
       61 tttacgtcac ccaagtagcg tgggtgtggt agctcgttgg tttggtgctt tgcataaatt
      121 agtcgggttg gaattaatta cccgcaaaaa gccggaaatt aactatccgg ttgtctatat
      181 cggcaatcat caaaacaatt acgatatggt aacgatttcc tcaatggtgg cagaaaagac
      241 ggtgagtatc ggcaaaaaaa gcctgatttg gattccgttt ttcggtttgg tttactgggc
      301 aaccggcaat atttttattc accgtgaaaa gcgttccagt gcgattgaaa caatgaataa
      361 agcagggcaa atcgtgaaag aacgcaatgt gtcggtgtgg atgttcccgg aaggtactcg
      421 tagccgtggg cgtggtttac tgccatttaa aaccggtgca ttccatttag ccatttctgc
      481 cggcgtgccg attgtgccgg tagtttgctc taatttacac aataaagtgg atttgaaccg
      541 ttcaaataac ggcacagtaa tctgtgaaat gcttgatccg attgatactt caggctattc
      601 ccgtgaaaac gtcaaagaac tgatggaaaa gtgccatcaa attatgttgg caaaatttga
      661 acaacttaat caagaagtcg aacagttaga aaacactgca aaaaacgcct aatatgaaaa
      721 aacagatcac ccgacgccaa tttttacaag gctctgccct tgcggctgga tttgcaacag
      781 tgccaacacc gctattagca gcagagcgac cgcaactgaa cattccgcct ttaatggata
      841 ttgggcgagg aaagccggtt cggttggatt tacgccccgc acaaacccaa tttgataaag
      901 gcaaattggt tgatgtatgg ggtgtgaacg ggcaatatct tgccccaacc gtacgggtaa
      961 aatcggggga ttttgtcaaa ttaacttatc tcaataattt accgcaaaaa atctcaatga
     1021 atattcaagg attgcaagcc tcaagtgaga tgattggcac ggtgcatcgt tcccttgacc
     1081 ccaaaagtag ctggtcgccg attgtttcta tcaatcaacc ggcttgtact tgttggtatc
     1141 acgccgatac aatgctcaac tcggcattcc aagtgtatcg tggcttagcc ggcttgtgga
     1201 tgattgaaga tgaacagagc aaaaaagcca ctttaccgaa taaatatggc gtgaacgaca
     1261 ttccgttaat tctgcaagac cagcttctca acaaagacgg cgtacaggtg ttagataaaa
     1321 acacctcaca atttttcggt aagcgattat ttgtcaacgg gcaggaatcg ccttatttta
     1381 acgtgcctcg tggctgggtc agattgcgga tcgtcaatgc ctcactttcc cgtgcttatg
     1441 aattacgctt agataacgag cagccgttgt acctaatcgc caccggctta ggaatgctgg
     1501 cagagccact tgaaaccgaa tcggttcaac ttgcaccgag tgaaagggtg gaagtgttgg
     1561 tcgatttaag cgaagggaaa gcggtatcgc tgataagcgg tcaaaaaaga gatatttttt
     1621 acaaaatcgg gcaggtcttt gctgacaacg acgaattgaa tgataacgtg attttagagc
     1681 ttcgccctga aggaatggca gcggtattga ataataaacc gagtttaccg gctttcaact
     1741 tagaggaatt taacctgaaa atcagccaag agagacgttt tgccattcgt ccggctgatt
     1801 atttaatcaa ccaaaagcgg tttgacccaa aacgcattga ttttaccgcg caaaaaggca
     1861 gcattgagcg ttggtattta catagcacac aagcggtcgg atttacgcta caaggtgcaa
     1921 aattcattat tgaaacccta aaccgcaagc aataccctat caaacaactt gcgtggcggg
     1981 acaccgtttg gctcgaggcg gagcaagaaa tcacgctgtt ggttcgcttt gataatactg
     2041 cccctgagca actgccattt acctttggcg tgagcgattt tatgctgcgt gatagaggta
     2101 cgatgggtca atttagcgta attgaaaatg gacaataacc gactgataag cgataatgcc
     2161 ccataatttt gcccataaaa aggaaaatag aatgagccaa gaatttttag attttgaatt
     2221 gccgattgct gaattggaag ccaagatcga gtctttaaga gcagtcagcg agcaagatga
     2281 caaaattaat cttgatgatg aaatcaagcg tttacaaaag aaaagcgagg aattaaccaa
     2341 gaaaaccttt gctaatttag atgcgtggca aatttcccgt atggcacgcc accccaatcg
     2401 cccgtacact ttagattata ttgagcatat tttcactgaa tttgacgaac tggcaggcga
     2461 ccgtgccttt gccgatgata aagcgattgt cggtggtatt gcccgtctgg acggacgtcc
     2521 ggtgatggtt atcggtcatc agaaaggtcg taccgtaaaa gagaaagtga aacgtaactt
     2581 tggtatgcct gctcctgaag gctatcgcaa agcgttacgt ttaatggaaa tggcagaacg
     2641 ctttaatatg ccaatcatca cctttatcga cacaccggga gcttatccgg gcattggtgc
     2701 ggaagagcgt ggtcagtcag aagcgattgc ccgtaacctg cgtgaaatgt ctcaactttc
     2761 cgtgccagta atctgtacgg taattggcga aggtggctcg ggcggtgcat tagcgatcgg
     2821 tgtgggcgat aaggtcaata tgttgcaata ttcaacttac tcggtaattt cacctgaagg
     2881 ctgtgcctcg attctgtgga aaagtgccga taaagcctca acggcagcag aagtaatggg
     2941 tttaactgcc cctcgtttaa aagagcttga gctgattgat aacatcgtgc cggaaccctt
     3001 aggcggtgct catcgcaatg ttgaacaaat cgcccaaaac cttaaacagc gtattgttga
     3061 agatttggca gggttagatc aacttaataa agaggattta ctggaacgcc gttatcaacg
     3121 cttaatgaat tacggttacg tctaattcaa tttcacccta caagcggtaa gatttttgtg
     3181 aaaatttgca aaatcttgcc attttacatt aaaggggact catcagaaaa taaaggtgat
     3241 ggtgccgact accggaatcg aactggtgac ctactgatta caagtcagtt gctctaccta
     3301 ctgagctaag tcggcatatt gaatggataa gagaacgagg cgggattata gagatttctt
     3361 gatgagcttc aagtccttgc aacttcaaat gactaaaaaa caacacatat tgaaatttta
     3421 aaggagtgaa tgaatgaaaa acatactatc catacaatcg cacgttgtct acggctatgc
     3481 cggcaataag gcttcaactt ttcctatgca gctgttaggt gttgatgtat gggcgttaaa
     3541 taccgtacag ttttccaacc atacccaata tggaaaatgg ggcggtatgg tgatgccaaa
     3601 agaacaaatc ggtagcattg ttgatggcat tcacaatatc ggtgaactgc atcgttgcga
     3661 tgccgtactc tctggctata tcggctcggc agaacaagta gccgaaatta ttcaggcttt
     3721 tcataaagtg aaagcggaaa atccaaacgc aatttatgta tgcgacccgg taatgggaca
     3781 ccccgacaaa ggctgcattg tggcagacgg cgtaaaagag ggattgatca atctggcaat
     3841 ggcacaagcc gacattatca caccaaattt agtggaatta agagaattaa ccggtttaac
     3901 ggtggaaaat tttgagcaag cggtcgaggc agtgaaagtg attttaacca aaggcccgaa
     3961 aaaagtattg gttaaacatt taagccgagt cggcaaagag agcaacaaat ttgaaatgct
     4021 gttcgccaac caagatggca tttggcacat cagccgccca ctctaccaat ttgccaaaga
     4081 acctgtcggt gtaggcgatt taaccgcagg gctatttgtg gcgaacttat taaatggaaa
     4141 atccgatcta gaagcctttg aacacactgc aaatgcggtc aatgaggtaa tggaacaaac
     4201 cgctagatca gggctttatg agttacaaat tattgcttct cgtgatcgga ttgttaatcc
     4261 acagtgtagt taccaagctg taaagattgc ctaattaaat gaagtataaa ataacattat
     4321 tatctctata actataaaaa aacgggcgta taaacgcccg ttttgtttaa aaagattcag
     4381 ttagaaatta attctaatcc cagcagtcac aacgttgttg tgaccactat tttttgcgag
     4441 tttaaggtcg taattcacat ataaggaagt atttttattt acttccgcca atgcaccaat
     4501 ccctacccac gttttatgtt ttgcaagccc aatacctttt acgtcgaatt ttgcatcttg
     4561 taaacctgta taacttgctt taaagctaag atcttctcga ttaaacgctt tttgatgatt
     4621 tacatagcct tggaaagtcg ttttcatccc agctaaattc acctctaaag atgttcgtaa
     4681 accaatcaat ccgcttgttt gtttataggt tgctttgtcc gcggtgagac caaattgact
     4741 gttttgctca ctgaatgcgc cacgtctgac aacatcatga ctaaaaccga caaatggagt
     4801 taacgcaaag cgaccttgct tgaagtcata gcctgattcg acataagcag aaaacactct
     4861 gtctcggtgg ttgatttttg ctgtacttgc gttattatcc gccaaaataa tatgacgctc
     4921 tacatctgaa tcaacactgc cataaccggc tcgaccttgt aaataccaag gtgttttatg
     4981 cttattgcct atacgggcat ataatgatgc acctattcca tctgcttttg aactaccgcc
     5041 ataacgatcg aagttcactt ttgctttaga ataattaagt gccgcaccta aaatcacatg
     5101 gtctgtaact ggcttatcaa aacctatttg gccagcatag gtattggttt taccttcacc
     5161 aaaccctatt tcttttagag taccattccc ataaataccc gttactcaca caccagcagt
     5221 ctcacctaca ttatccagcg ttcctaacat tacaagacgg ttagagagat ctttattcac
     5281 tgtttctgag ttttcaaaag taagcgcttg cgcagacgca taaatttgac cagataagct
     5341 atctaaaacc acactttgcg tattgatgtt tgcaatactg ttttgtaaag tagccgcttg
     5401 ttgtgcgaat tcatcatttg tagcatttgt agaaccatta tttccttcaa tttggttatc
     5461 caatgccgaa aatgagctct ctaaagcagt cgcgacattt ttccacattg catcaccaga
     5521 ggtgttttcg tttacatagt ctgctacatt tttccggcta actgttgctt ttacttcatt
     5581 ctcattggtt ttttccgccg tcgcagttaa taaccctgag gtttctattg cagcaaagtt
     5641 acccttaatt gcggaatcag aagtaatagc actcattgat aaaccttttt gggtcacata
     5701 ctgagcttta ccctctttta ccgcagctag tgataaagta gagccgttta
//
LOCUS       DANJWC010000143         5742 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.143, whole genome shotgun sequence.
ACCESSION   DANJWC010000143 DANJWC010000000
VERSION     DANJWC010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5742
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.143"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            13..69
                     /locus_tag="PWX02_001982"
     CDS             13..69
                     /locus_tag="PWX02_001982"
                     /inference="COORDINATES: protein motif:HMM:NF016757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PT domain-containing protein"
                     /protein_id="HDL1729961.1"
                     /translation="MRVNQPTNQPTNQPTNQL"
     gene            87..899
                     /locus_tag="PWX02_001983"
     CDS             87..899
                     /locus_tag="PWX02_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL1729962.1"
                     /translation="MGRPELERAVLSVQNQTYPCKHYVFVDGEKYHQQAADILKNFPS
                     VIVTYLPMNTGADGWVNSSINAIALFLVKEDIICYLDDDNWFEPTHVESGVHALLEMQ
                     SDYAYSLRNFYDLDGEFLCIDTIESLGEYNCVYQEPLECNYQLGGVKGVLSINLNDCQ
                     HIDTNCYFIRSEIARLISTAWYTGIHNDRNVYKKLQELALKGNCTKKVTVNYTLDTRK
                     YYGSVFNELLEEPFLLSEYDAYQFTNEVIKFHSEESLKAWGGCLIWEQNNGS"
     gene            1031..1729
                     /gene="aphA"
                     /locus_tag="PWX02_001984"
     CDS             1031..1729
                     /gene="aphA"
                     /locus_tag="PWX02_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HDL1729963.1"
                     /translation="MTAATMFASLIAFSAQAGGPKVAYTQPGIDMRTHEAQQAPIHWV
                     SIEQIKESIKDLPPMAVSFDIDDTVAATSGCFHYGKQKYSPDDFSYLKNQDFWDEINA
                     GCDKYSLPKQVAINLIKMHQERGDQVYLITGRTAGKNEQVTTIMQKYLGIKNMQPVNF
                     MGGKAHSTKYDKTPAIIEHKVQIHYGDSDDDILAAREAGIRGIRILRAANSNYTPFPQ
                     AGGYGEEVVVNSSY"
     gene            1910..2671
                     /gene="udp"
                     /locus_tag="PWX02_001985"
     CDS             1910..2671
                     /gene="udp"
                     /locus_tag="PWX02_001985"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HDL1729964.1"
                     /translation="MSEVFHLGLTKAMLKGAKIAIVPGDPARSERIAKKMDNPEFLAS
                     TREFTSWLGYLDGEPVVVCSTGIGGPSVSICVEELAQLGVRTFLRIGTTGAIQEHINV
                     GDILITTGAVRLDGASQHFAPMEYPAVANFECTNALYAAAVESGVTPYVGITASSDTF
                     YPGQERYDTFTGKIYRHFQGSLKQWQDLNVMNFEMESATLFTMCSALGLRAGMVAGVI
                     VNRTQQEIPNEATIHDTEHKAVSVVVEAARKLIKG"
     gene            2746..3342
                     /locus_tag="PWX02_001986"
     CDS             2746..3342
                     /locus_tag="PWX02_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729965.1"
                     /translation="MKKWLMLITLIFGITACFSSLEFDESKQEKINSFLIVGNKLYVV
                     GELHDYEFEHQQVAELNNFLKSLIANKIKSVEANFEVKESKKVEGSYRIILDGKSLTE
                     SERSTVMDTFHFNSAQNPSITYQAEGNLVKLANREELLQKFRFKQALEANVRYYKMKS
                     GLQNTAEGALSIVMLPVAVVAVLPFYMFWVGACATYGC"
     gene            3353..3838
                     /locus_tag="PWX02_001987"
     CDS             3353..3838
                     /locus_tag="PWX02_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HDL1729966.1"
                     /translation="MYSLYLKILSECDWCCDPKTLSTYQQEWLLHEGSLTQKLYDVAT
                     NFNVEVVQEKWIAKNSEKTTACNEDVWSREVLLKDGEKNWIFAQTLVPRSTIENIAQN
                     VLELGNQPIGLWLFSQNPTRTKLEWTQTENGLFARRGVYEIRGYSLEIRELFLETFPY
                     L"
     gene            complement(3876..4745)
                     /locus_tag="PWX02_001988"
     CDS             complement(3876..4745)
                     /locus_tag="PWX02_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL1729967.1"
                     /translation="MKNINTQAVGWIGLGQMGEPMAKHLLNSGVNVGVYNRDPAKAEK
                     VVEAGGTLYPSVLELVKAYDVIFLMIADYAAVQQVLSDEVLAELNGKVVVNMSTISPS
                     QNQAVEALLAQHGAEFVEAPVSGSSKVAEAGKLLVLAAGKEETVELLKPLFAAFSTTT
                     FYYGEVGKAGGIKLMINSLLGIFIQAYGEALNFADQYGIPKEKVIEMISGSFMNSPIF
                     QAKVPMYQANEFPPAFMMKHMTKDFNLASEEIAKLGKSYPLIEQATKTYNQANDSGLS
                     EVDMAAIYQFLAK"
     gene            4875..5666
                     /locus_tag="PWX02_001989"
     CDS             4875..5666
                     /locus_tag="PWX02_001989"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDL1729968.1"
                     /translation="MKPTILLYDSGMGGLTIYDEIRKVLPHAHYLYCFDNAFFPYSEK
                     SEAVLIERAVKIVQKIAEKQPLDMVVVACNTASTVVLPALREHFNFPIVGTVPAIKPA
                     AAISETKVIGLLATKGTVARPYVDELIRKYAQDCTVEKIGTTDLVEIVEEKQRSGKVD
                     MNRLQKVVEAWQGKPELDTVILGCTHFPLVKAELQQLLPDVKYFVDPGVGIAKRVLSL
                     LEERKFQQKADFENQAFCTKQSSAFNIKQDVMQNWGFNNLSVIDI"
BASE COUNT         1796 a         1013 c         1188 g         1745 t
ORIGIN      
        1 ataggaaata gaatgcgtgt caaccaacca accaaccaac caaccaacca accaaccaac
       61 caactgtagc tgttatcagt tcaacaattg gtcgtcctga attagaaaga gctgttttaa
      121 gcgtacaaaa tcagacttat ccttgtaaac attatgtttt tgttgatgga gagaaatatc
      181 accaacaagc ggctgatatt ttaaagaatt ttccaagtgt tattgttact tatctcccaa
      241 tgaataccgg ggctgatggt tgggtaaata gcagcattaa tgcaattgca ctatttttag
      301 tgaaagaaga tattatttgt tatctggatg atgataattg gtttgaacct actcacgttg
      361 aatctggtgt gcatgcttta ttagaaatgc aatctgatta tgcttactct ttacgtaatt
      421 tttatgattt agatggcgag tttttgtgta ttgatacgat tgaatcgttg ggagaatata
      481 attgcgttta tcaagaacca ttagaatgta attatcaatt aggtggtgtt aaaggtgttt
      541 tatcaataaa tctgaatgat tgtcagcata ttgatacgaa ttgctatttt attcgtagtg
      601 agatagctcg ccttatcagt acggcttggt acacaggtat tcataatgat agaaatgtat
      661 ataaaaaatt acaagaattg gcactaaaag gaaattgtac aaagaaagtt acggttaatt
      721 atactcttga tacacgtaaa tattatggat cagtatttaa tgaattatta gaggagccat
      781 ttttattaag cgaatatgat gcctatcaat ttacgaatga ggtgattaaa tttcattcag
      841 aagaaagcct taaagcttgg ggaggttgct tgatttggga acagaacaat ggctcataag
      901 cgttaagcgt aatcgtttgc ttgtgatcat attcacaaaa taaggttttt gtattagcta
      961 agagtgccta ataagattaa aataggtatg gttaatcata tggaggtatt ttataaaatt
     1021 tacattaaaa atgacggcag cgacaatgtt tgcatctctt attgcttttt cagcacaagc
     1081 aggcggacca aaagtagctt atacccagcc gggaattgat atgcgaacac atgaagcaca
     1141 gcaagcgcca attcattggg tatctattga gcaaattaaa gaaagcatca aagatttacc
     1201 ccctatggcg gtaagttttg atattgatga tactgttgct gcaactagcg gttgcttcca
     1261 ctacggaaag caaaaatatt cgccagacga tttcagctac ttaaaaaatc aagatttttg
     1321 ggacgaaatt aatgctggct gtgataaata ttctttgcca aagcaagttg ctattaattt
     1381 aataaaaatg caccaagaac gtggtgatca agtttattta attacggggc gtaccgcagg
     1441 taaaaatgaa caagttacaa cgattatgca aaagtatctt ggtattaaaa atatgcaacc
     1501 ggtaaatttt atggggggga aagcacattc aacaaaatat gataaaaccc ctgcgattat
     1561 tgaacataag gtgcaaattc actatggtga cagtgatgat gatatcttag ctgcccgtga
     1621 agccggtatt cgaggtattc gtatacttcg tgcagcaaat tctaattata cgccattccc
     1681 tcaggccgga ggatatggtg aagaagttgt tgtcaattca agttattaat ttcttagtct
     1741 gaaatatggc caccattgcg tggccatcat tttttattta tataatgacg aaaccgttta
     1801 cctttgatct agttcacaaa atcaaacttt gcttgcaaaa aattgtcgta aattaaccgc
     1861 ttgctaagat agaatggctc atatccagtt agataaaaag gagataaaaa tgtctgaagt
     1921 atttcattta ggtttaacca aagccatgct caaaggtgca aaaattgcaa tcgttccggg
     1981 cgatccggct agaagtgaac gtattgccaa aaaaatggac aacccagagt ttttagcctc
     2041 aacccgtgaa tttacctctt ggttaggtta tttagatggc gaaccggtgg tggtttgttc
     2101 aaccggtatt ggcggaccgt cggtgtcgat ttgtgtggaa gagttagcac agttaggtgt
     2161 tcgcacattc ttacgcattg gcacaacagg tgcaattcaa gagcatatta atgtaggcga
     2221 tattttaatt accaccggag cggtgcgttt agatggtgcg agccaacatt ttgcaccaat
     2281 ggaatatcct gcagtagcga attttgaatg tactaatgct ctttatgcag cagcagtgga
     2341 atcaggcgtt acaccttatg tggggattac agcttcatca gacactttct acccggggca
     2401 agaacgttac gacactttca ccggcaagat ctatcgtcat ttccaaggtt cattaaaaca
     2461 gtggcaagat ctcaatgtta tgaattttga aatggaatcc gccactctct ttacgatgtg
     2521 ttcggcatta ggcttgcgag caggtatggt tgctggtgta attgtaaacc gcactcaaca
     2581 agagattccg aatgaagcaa ccattcacga caccgaacat aaagccgttt cggtagtagt
     2641 ggaagcggca agaaaattaa ttaaaggcta atctttaaat ttaaaagcaa aaaaggtaaa
     2701 ttgtctttgt tacaatttac cttttttctt tttagggagc tttctatgaa aaaatggttg
     2761 atgttaatta cattaatttt cggcattacc gcttgttttt ccagtttaga atttgatgaa
     2821 agtaaacaag aaaaaatcaa ttcattcttg attgttggta ataaattata tgtggtgggt
     2881 gaactgcacg attatgaatt tgagcatcag caagttgcgg aactgaataa ttttctcaaa
     2941 agcctaattg ctaataaaat caaatctgtt gaggcaaact tcgaggttaa ggaatccaaa
     3001 aaagtggaag gtagttatag aattatttta gatgggaaat cacttacaga aagtgaaaga
     3061 agtacggtga tggatacctt tcattttaat tcagctcaaa atccatctat tacttatcag
     3121 gcggaaggga acttggtgaa attagccaat agagaagagc ttttgcaaaa attccgattt
     3181 aaacaagcgc ttgaggcgaa tgtgcgttat tacaaaatga aaagtggtct tcaaaatacg
     3241 gctgaaggag cgttaagtat tgtaatgttg cctgttgctg tagtagctgt tttaccattc
     3301 tatatgtttt gggttggtgc ttgtgccact tacggatgtt aaataacttt ttatgtattc
     3361 tctatacctg aaaattttat cggaatgcga ttggtgttgt gatccaaaaa cactttctac
     3421 ctaccagcaa gaatggcttc tgcacgaagg gtcacttacc caaaaattgt atgatgtcgc
     3481 aaccaatttc aacgtggaag tagtacaaga aaagtggatt gcaaaaaaca gtgaaaaaac
     3541 gaccgcttgt aatgaagatg tttggtcgcg agaagtgtta ctgaaagatg gtgaaaaaaa
     3601 ttggattttt gcccaaactc ttgtgccaag atcaacaatc gaaaatatcg ctcaaaacgt
     3661 gttggaattg ggcaatcagc cgattggttt atggctattt tctcaaaatc ctactcgcac
     3721 caaattagaa tggacacaaa cagagaacgg tttattcgct cggcgtgggg tgtatgaaat
     3781 tagagggtat tctctggaaa ttcgagagct ttttttagaa acctttcctt atttataaaa
     3841 aaatcggttg gtaatgacac caaccgattt tcatcttatt ttgccaagaa ctgataaatt
     3901 gcagccatat ccacttcgct caagcctgaa tcattggctt gattgtaggt tttagtggct
     3961 tgctcaataa gcgggtaaga tttgcctaat ttcgcaattt cttcgcttgc caaattaaaa
     4021 tctttggtca tatgtttcat cataaacgca ggcggaaatt cattagcttg gtacatcggc
     4081 acttttgctt ggaagatcgg gctgttcata aacgagcctg aaatcatttc gattactttc
     4141 tctttcggaa tgccatattg gtcggcgaag tttaacgcct cgccataggc ttgaatgaag
     4201 atgcccagta gcgagttgat catcaattta atcccaccgg ctttacccac ttcaccataa
     4261 tagaaagtgg tggtactaaa ggcagcgaaa agcggtttga gcaactcaac ggtttcttct
     4321 ttaccggcag ccagtaccaa tagtttacct gcttctgcca ctttgcttga accggaaacc
     4381 ggagcttcaa caaattctgc accgtgttgg gctagtagag cttctaccgc ctggttttgg
     4441 cttggggaaa tggtgctcat attcaccact actttgccgt ttaattcagc caacacttca
     4501 tcgcttaaca cctgttgcac ggcggcatag tctgcaatca tcaagaaaat cacatcgtag
     4561 gctttcacca attccaacac agaaggatat aatgtgccgc ctgcttcaac taccttttct
     4621 gcttttgctg ggtcgcggtt gtaaacgccc acgttcactc cgctatttag caaatgtttt
     4681 gccatcggtt cgcccatttg ccctaaacca atccaaccga ctgcttgtgt attaatgttt
     4741 ttcattattt ttctccttac tgaataaagc aaattgatta ttgcgatttt ccctcatcaa
     4801 gtcaaataga gagaacttga atatagcaat ggaaaagagt agcattttag cacttacttt
     4861 tttgaacaat accaatgaaa ccaacaattc ttttatacga ttccggaatg ggcggattga
     4921 cgatttacga tgaaatccga aaagtgttac cgcacgctca ctatttatat tgctttgata
     4981 acgctttttt cccatattcc gagaagtcgg aagcagtttt aattgagcga gcggtcaaaa
     5041 ttgtgcaaaa aattgcagaa aaacaaccgc ttgatatggt tgttgttgct tgtaatacgg
     5101 caagcacagt ggttctgccg gcattgcgtg agcattttaa ttttccgata gtaggaactg
     5161 taccggcaat taaaccggca gcggcaattt ccgaaactaa agtcatcggc ttgctcgcca
     5221 ctaaaggcac ggttgctcgc ccttatgttg atgaattgat aagaaaatat gctcaggatt
     5281 gtacggtaga aaaaattggc acaaccgatt tggttgaaat tgttgaagaa aaacaacgaa
     5341 gcggtaaagt ggatatgaac cgactacaaa aagtggtcga ggcttggcaa ggtaagccgg
     5401 aattggatac ggttatactc ggttgtaccc attttccctt agtcaaagct gaattacagc
     5461 aattattgcc cgatgtgaaa tattttgttg atccgggtgt aggtattgcc aaacgggtgc
     5521 taagcctttt ggaagaacgc aaatttcaac aaaaagccga ttttgaaaat caagcattct
     5581 gcactaaaca aagttctgca tttaatatca aacaagacgt tatgcagaat tggggattta
     5641 ataatctttc ggtgattgac atttaaacaa aacaatctgt tttgatgaaa aaaggttcag
     5701 ttgagcaaaa actttaaaaa agggcttgca ctcaatttta aa
//
LOCUS       DANJWC010000144         5724 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.144, whole genome shotgun sequence.
ACCESSION   DANJWC010000144 DANJWC010000000
VERSION     DANJWC010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.144"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            75..1232
                     /gene="hemW"
                     /locus_tag="PWX02_001990"
     CDS             75..1232
                     /gene="hemW"
                     /locus_tag="PWX02_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HDL1729969.1"
                     /translation="MNLILPPLSLYIHIPWCVQKCPYCDFNSHAQKGLIPEQEYIEHL
                     LADLSQDLTAYRVAIGDRKLYSIFIGGGTPSLFSAEGIAYLLSEVEKRIAFEPNIEIT
                     LEANPGTAEAERFLGYAQGGVTRISMGIQSFEPEKLLKLGRIHDANEAKQAIKFAQNS
                     AKSGLQSFNIDLMHGLPNQSVKQALVDLETGISLNPPHLSWYQLTIEPNTMFYYRKPT
                     LPDDDELWDIFEQGHQLLTNAGYEQYETSAYAKKGYQCRHNLNYWRFGDYLAIGCGAH
                     GKISYPTGEIYRFSKTKHPKGYMRGEYRYSQDLIELDDRPFEFFMNRFRLLEATPKQE
                     FEWFTGLDREIVRPTIDWALNQNYISESSTHWQITQHGKLFLNELLEGFLD"
     gene            1374..2022
                     /locus_tag="PWX02_001991"
                     /pseudo
     CDS             1374..2022
                     /locus_tag="PWX02_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824574.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
     gene            2109..2576
                     /gene="bcp"
                     /locus_tag="PWX02_001992"
     CDS             2109..2576
                     /gene="bcp"
                     /locus_tag="PWX02_001992"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDL1729970.1"
                     /translation="MNILQAGDKAPQFTLQNQADESVSLSQFAGKKVLVYFYPKALTP
                     GCTTQACGLRDSKSELDELNVVVLGISTDLPKKLAQFVEKKALNFTLLSDPEHQVAEA
                     FGVWGEKKFMGKTYDGIHRISFLIDEQGVVEQVFDKFKTGEHHQMIVDYLRGR"
     gene            2576..3124
                     /gene="gmhB"
                     /locus_tag="PWX02_001993"
     CDS             2576..3124
                     /gene="gmhB"
                     /locus_tag="PWX02_001993"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HDL1729971.1"
                     /translation="MGKKTIFLDRDGTINIDHGYVHQIDDFQFIDGVGKALKQLQDKG
                     YLLVLVTNQSGIARGYFSEAQFMQLTEWMDWSLDEDYGVVLDGIYYCPHHPEGKGEYR
                     EDCDCRKPKAGMFTQAIQDLNIDPSQSYMVGDKLEDILAAEAAGVKTKVLVRTGKAVT
                     EEGQAKADLVLDSLVDLVKYIK"
     gene            3129..3968
                     /gene="queF"
                     /locus_tag="PWX02_001994"
     CDS             3129..3968
                     /gene="queF"
                     /locus_tag="PWX02_001994"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDL1729972.1"
                     /translation="MNYNDKSLSALKLGQKTEYKSEYDASLLQPVPRKLNRDGLGITQ
                     EQPFNRGADVWTCYELSWLNQNGLPQVAIADVAIDFKSENLIESKSFKLYLNSFNQTK
                     FSSIEEVEKTIQADLARCASGQVSVKIYQLSHYTQQPIVDFAGDCIDEQDIQIDSYAF
                     STQHLENIAENEPVEEILVSHLLKSNCLITSQPDWGSVQIRYKGKKLNCEKLLRYLVS
                     FREHNEFHEQCVERIFCDLMQFAQPEKLTVYARYTRRGGLDINPFRSNFEEVPENLRM
                     ARQ"
     gene            4160..5293
                     /locus_tag="PWX02_001995"
     CDS             4160..5293
                     /locus_tag="PWX02_001995"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDL1729973.1"
                     /translation="MLIFMMNEYSPNYYIGVMSGTSLDGVDIALLDFAKNPPKMTACD
                     FFPMPEELRADISALLKTGETNLQKLGEIDHRLGLLYAETINAFLAKNQLKAEDIQAI
                     GCHGQTVWHSPDSEYPFTTQIGDMNLVAEQTGITTIADFRRKDMAVGGQGAPLVPAFH
                     QAIFSDPNRLTVVLNIGGISNISVLNPNSPTVGYDVGAGNTLMDSWIEQHQGKRYDKN
                     GEWARTGKLNTELLATLLDESFFAKAPPKSTGRELFNLTWLAKKLENLTACQPQDVQR
                     TLAEFTAQSIANELLRFESSLPRLLLVGGGGARNPLLMERISALLPEWEVTTTTEFGL
                     DIDYVEAAAFAWLAYQRIHNLPSNMPSVTGAKKAVSLGVIYPA"
     gene            complement(5290..>5724)
                     /locus_tag="PWX02_001996"
     CDS             complement(5290..>5724)
                     /locus_tag="PWX02_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1729974.1"
                     /translation="LFIPLGLGSAFYIIFPYIFTNLLDKPELVSVSFFVNNLIAVLLQ
                     SDFSRKLNLTVHKLIYIAPILIILQIMPWFYALQWLSTITAFLYLVIFATITVYANTA
                     LSNMLVKLDNGKNQGLMFGSSKLILAITTLGVMNVLPYIFLI"
BASE COUNT         1792 a         1070 c         1260 g         1602 t
ORIGIN      
        1 cgaaaatctt tgcttttcac ttcacgtgct tttcgttatt attcgccaat ttaatcataa
       61 atgaaaaatt gacagtgaac ctgattttac cgcctttaag cctttatatc catatccctt
      121 ggtgcgtgca aaaatgccct tattgcgatt ttaactcgca cgctcaaaaa ggattaattc
      181 ccgagcaaga atatatcgaa catttacttg cagatttatc ccaagatttg accgcttacc
      241 gagtggcaat aggtgatcgt aaattatatt ctatttttat tggcggcggc accccgagct
      301 tattttcagc ggaagggata gcttatttat taagtgaagt agaaaaacgg attgcattcg
      361 agccaaatat cgaaattaca ctggaagcga acccgggaac cgcagaggca gagcgatttt
      421 taggttacgc acaaggtggc gtaacccgca tttcaatggg aatccaaagt tttgagccgg
      481 aaaaattatt gaaattagga cggattcacg atgcaaatga agcaaaacaa gcgattaaat
      541 ttgcccaaaa ttctgcaaaa tcgggcttgc aaagtttcaa cattgattta atgcacggct
      601 taccaaacca atcggttaaa caagccttgg tggatttaga aacagggatt tcactcaatc
      661 caccgcattt atcgtggtat cagctcacga ttgagccgaa tacgatgttc tattatcgta
      721 agccaacctt gcctgatgat gacgagttat gggatatttt cgagcaaggg catcaacttt
      781 taacgaatgc cggctatgag cagtatgaaa cctcggctta tgccaaaaaa ggctaccaat
      841 gcagacacaa tttaaattat tggcgatttg gggattattt ggcgattggt tgtggtgctc
      901 acggcaaaat ttcgtatccg acaggggaaa tttatcgttt tagcaaaacc aaacacccga
      961 aaggttatat gcgaggggag taccgctaca gccaagatct gattgagtta gacgaccgcc
     1021 cgtttgagtt ttttatgaac cgctttagat tgcttgaagc cacgccaaaa caagaatttg
     1081 aatggtttac cggtttagac agggaaattg ttcgcccgac aatcgattgg gcgttaaacc
     1141 aaaactatat ttccgaaagc tcaacccatt ggcaaattac ccaacacggt aaattgtttt
     1201 taaatgaatt actggaaggc tttctagact gatttgcaaa aaaacgacca aaacagaccg
     1261 cttgtaatgc taaattacag gcgttttttg tgatgtgcgt cgaaaaattc gtatttttta
     1321 ctagacaaaa aaacagtatt cagtagcata gctctcacaa ataaggagag cttatgaccg
     1381 ataaacaaat ccgtaatagt accgccgaat ttttgatttt tacccaacaa aatggagcag
     1441 aaaatattac tgttcgttat gaagataacg aaatttggct cacgcaaaaa ttaattgctc
     1501 agctttttga tgtttctatt ccaactatca atgagcattt aaagcatatt tttgaaagtg
     1561 gagagctaga agaaatgtca gttattcgga atttcctaat aactgccgca gatggtaaaa
     1621 attatcgtac caaacactac aaccttgatg ccgttatttc gataggctat agggtaaact
     1681 cggtgcgggc gacacaattt cgccaatggg caactaaagt attacgggat ttcgcccaaa
     1741 aaggctatgt gattgaccgc caaagaatgg aaaatggtac atttttaaat gaagattatt
     1801 ttgagcagtt gctggcagaa attcgtgaaa tttggttatc tgaacggcgt tcttgagggc
     1861 aaaggcaaaa tgcgttttga tgaagcgaaa ttgcacgcag aaagcgaatt tgagaaatac
     1921 cgcattgttc aagacagatt atttgaaagc gattttgaca aggtattgaa agaagtgttg
     1981 cggtcggaaa atcaataccg tccatcattt tcaacaacat aaaacaaccg cattttgcaa
     2041 attttttgcc aagtttgacc gcttgccgtg tataatgcaa gcggttattt tttatggaaa
     2101 aattacaaat gaatatctta caagcaggcg ataaagcccc tcaattcact cttcaaaatc
     2161 aggctgatga gtcggtttca ctctctcaat ttgccggtaa aaaagtgttg gtttatttct
     2221 acccgaaagc ccttacaccg ggttgcacta cgcaagcttg tggtttgcgt gacagcaaat
     2281 cagaacttga tgagctcaat gtggttgttc tcggaattag caccgattta ccgaaaaaat
     2341 tagctcaatt tgtagagaaa aaagccctta attttacctt attatctgac ccggaacacc
     2401 aagttgcgga agcctttggt gtatggggcg aaaagaaatt tatgggaaaa acctatgacg
     2461 gcattcaccg catcagcttt ttaattgatg aacagggcgt ggttgagcaa gtgtttgata
     2521 aatttaaaac cggcgaacat caccaaatga tcgtggatta tctacggggt cgctaatggg
     2581 aaaaaaaacc atttttttag accgtgacgg cacaattaat atcgatcacg gctatgtgca
     2641 tcaaattgat gattttcaat ttattgacgg tgtcggcaag gcgttaaaac agctacaaga
     2701 caaaggttat ttattggtgc tggtaaccaa tcaatctggc attgcccgag gctattttag
     2761 cgaagcacaa tttatgcaac taaccgaatg gatggactgg tcgctcgatg aagattatgg
     2821 cgtggtacta gacggtattt actactgccc acatcacccc gaaggcaaag gcgaataccg
     2881 tgaagattgt gattgccgta agcctaaagc aggtatgttt actcaagcga ttcaagattt
     2941 aaacatcgac ccaagccaat cttatatggt gggcgataaa ttagaagata tccttgccgc
     3001 agaagctgcc ggcgtgaaaa ccaaagtgct ggttcgcacc ggcaaagccg tgacggaaga
     3061 aggtcaggct aaagccgatt tggtgttaga tagtttagtt gatttagtaa aatatattaa
     3121 ataaagcaat gaattataac gataaatctc tttcagccct gaaattaggg caaaaaaccg
     3181 agtacaaaag cgaatatgac gcctcgcttt tgcaacctgt tcctcgtaaa ttaaaccgtg
     3241 atggtttagg cattacacaa gagcagcctt ttaaccgagg ggctgatgtg tggacttgct
     3301 atgagctatc ttggttgaac caaaatgggc taccgcaagt agcgattgca gatgtcgcca
     3361 ttgattttaa gagcgaaaac ttaattgaat cgaaaagttt caagctctat ttgaacagtt
     3421 ttaaccaaac caaatttagc tcaatagaag aagttgagaa aacgattcaa gcggatctgg
     3481 ctcgctgtgc aagcggtcag gtttcggtaa aaatttacca attatcgcac tacacgcaac
     3541 agccgatagt tgattttgca ggcgattgca ttgatgagca agatattcaa atcgacagtt
     3601 atgcgttttc gactcaacat ttagaaaaca ttgcagaaaa tgagccggtg gaagaaatct
     3661 tagtcagcca tttgctcaaa tctaactgct taattacttc acagcctgac tggggaagtg
     3721 tgcaaattcg ctacaaaggc aaaaagctaa actgtgaaaa actgttacgt tatttagtgt
     3781 cgttccgtga gcataatgag ttccacgaac aatgtgtgga acgcattttc tgcgatttaa
     3841 tgcaattcgc tcagccggaa aaattaacgg tttacgcacg ctacaccaga cgaggcgggc
     3901 tagacatcaa tccgtttaga tcgaattttg aggaagtgcc ggaaaatcta agaatggcac
     3961 gccaatgatt ttgttgtttt ttgttcccgt ctgaaccgcc tgagcgtgtt tgaattggta
     4021 ggtaaattta ctctgagcgg agcgaatgta aatttgccgt taagccaatt caaactaagc
     4081 gaggatagcg gtgaagctcg ggttgccttt cttttggtta cttttctttg gcaaagcaaa
     4141 gaaaagtaac aaatagcaga tgttgatttt tatgatgaat gaatatagcc caaattacta
     4201 tataggcgta atgtccggca cgagccttga tggggtggat attgctttgc tggattttgc
     4261 aaaaaatcca ccgaaaatga ccgcttgtga tttttttcca atgccggagg aattacgggc
     4321 ggatatttct gcgttactta aaaccggcga aaccaatctc caaaaactgg gggaaattga
     4381 tcaccgttta ggcttgcttt atgcagaaac gatcaatgcg tttttagcga aaaatcagct
     4441 aaaagcagag gatattcaag ccataggttg tcacgggcag acggtgtggc attcaccgga
     4501 ttctgagtat cccttcacca cccaaattgg cgatatgaat ttggtggcag agcaaaccgg
     4561 tattaccact atcgctgatt ttcggcgtaa agatatggca gtaggtgggc aaggggctcc
     4621 gcttgtgccg gcatttcatc aagctatttt tagcgatcca aatcgtctaa cagtggtgct
     4681 gaatattggc ggcatcagca atatttcggt attaaatccc aattccccta cagttggtta
     4741 tgatgtagga gccggaaata ctttgatgga tagttggatc gaacaacatc aaggcaaaag
     4801 atacgataaa aacggcgaat gggcgagaac gggcaagctc aatactgaac tgttagcgac
     4861 gttattagac gagtcgtttt ttgctaaagc cccgcctaaa agcactgggc gggagctgtt
     4921 taatttaacg tggcttgcaa aaaaattgga aaatttgacc gcttgtcagc cacaagatgt
     4981 gcaacgcacg ctggcagaat ttactgctca aagtattgcc aatgagttat tacgctttga
     5041 aagctctctg cctcgcttgc ttttagttgg tggtggtggg gcaagaaatc cgttattaat
     5101 ggaacggatt tctgctttac ttcccgagtg ggaggttaca accactacag aatttggttt
     5161 agatattgat tatgtagagg ctgccgcttt tgcttggtta gcttaccagc gaatccataa
     5221 tttacccagt aatatgccta gcgtgacagg agcaaaaaaa gccgtgagtt tgggcgtgat
     5281 ttaccctgct tagattaaga aaatgtaagg taatacattc attactccga gagtggtaat
     5341 agctaaaatt aatttagagc ttccaaacat taagccttgg tttttaccat tgtctaattt
     5401 cactagcata ttagatagtg ccgtattcgc atacacggta atagtggcaa aaatcactaa
     5461 atagagaaat gcagtgatgg ttgaaagcca ttggagtgca taaaaccaag gcattatttg
     5521 aagtataatc agtattggtg caatataaat aagtttatgt actgtcagat ttaattttcg
     5581 tgaaaaatca gattgcaata acactgcaat taaattattc acaaagaatg agacagagac
     5641 taattctggt ttatccagaa gattggtgaa tatatagggg aatataatat aaaatgcact
     5701 tcctagacca agcggaataa aaag
//
LOCUS       DANJWC010000145         5673 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.145, whole genome shotgun sequence.
ACCESSION   DANJWC010000145 DANJWC010000000
VERSION     DANJWC010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.145"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            256..735
                     /locus_tag="PWX02_001997"
     CDS             256..735
                     /locus_tag="PWX02_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HDL1729975.1"
                     /translation="MDNLFDLKLNINQIAELTGLHRQTVSQRVAGLTPSLGSNSKLKL
                     YALRDLILTGLAEKMSADVDSLSPVDRKSFWQAENERLKYEEKTGELIPASEVAFEMG
                     AMAKAVVQTLETLPDILERDCGLQPKDLIRVQQVIDDVRDQMALHIQQAPTTEQGDD"
     gene            735..2846
                     /locus_tag="PWX02_001998"
     CDS             735..2846
                     /locus_tag="PWX02_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010786315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HDL1729976.1"
                     /translation="MFASAKEIRRDMANAVKAPRRMKVSDAVAEYMRVPLGGGNSVKW
                     DKHTAAYMLEPMDCLNSREYDAVIFVGPARTGKTIGLIDGWITYSIICDPSDFLLVQL
                     TQEKASEHSRKRLDRTFRGSPEIAKRLSPRKNDNNVHDKYFRAGNLLKIGWPSINVLS
                     SSDYKYVALTDYDRWPEDVDGEGDGFSLASKRTTTFMSAGMTLVESSPGKDIVDLKHI
                     PKSTHEAPPTTGILSLYNRGDRRRFYWQCPCCSEYFEPSMANMVGYREESDFVKASEN
                     ARLQCPHCQALIPPELKRELNIKGVWLKEGQKIDASGQISGESRKSRIASFWLEGPAA
                     AYQTWAQLTYKLLNAEHEYEMTGSEETLKAVTNTDWGLPYLPRSALEQRRSDELMERR
                     EEVEEKTVPPQCRFIVAAVDVQGGKNRRFVVQMVGYGENGERWLIDRYNISHTLPDRD
                     GVIEKIDPRIPEDWHILISDVLEKRYVLAHKPSDFMPILAMAVDSGGEEGVTDNAYQF
                     WRKCRRDGYAKRVYLVKGDSTKRQKLITKTHPDNTSRSDRHASARGDVPLYLLQTDYL
                     KDRINNALSRDTVGANYIHFPDWLGEWFFNELTYEERGADGKWRKPGKGNNEAFDLFC
                     YAHAIAILRGYERIKWGDENDVPSWAKLPDINPNIIRETPRTAEQAVEIEEKPAKPQP
                     KPTKAKSSWLSGGGRKTGGWL"
     gene            2843..3067
                     /locus_tag="PWX02_001999"
     CDS             2843..3067
                     /locus_tag="PWX02_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729977.1"
                     /translation="MSLYTIDELKDKIRTLDEKIETAQSQVSFNGRSVSYQVAELTKQ
                     RDRYQTMLDEQLAQSGQHTKQHRIKYARFI"
     gene            3067..4578
                     /locus_tag="PWX02_002000"
     CDS             3067..4578
                     /locus_tag="PWX02_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL1729978.1"
                     /translation="MNFLEKTIATLSPKWAANRSRNRYVLNAYEAAQPSRTHKASRES
                     KGANSTVRQSAVSLREQARALDQNHDIVIGILDKMEERVIGSKGIHIEPQPLTLAGDV
                     HEDLAEQIRKLWAEWSVKPEVTGLYTRPLLERMLLRTWLRDGEVFVQLVKGKVAGLEH
                     GSPIAFSLEALEPDFVPMQSDEAKNGLVQGVFLNAWRKPTAYQVYLDNPQESNGMYGK
                     VKTVSAENMLHLAFRKRLHQIRGVSMLHGVIVRLADLKEYEESERVAARIAAAMTMYI
                     KKGDAALYDDDSNSDGNRLFDIAPGAVIDDLKPGEDIGLINSNRPNVNLETFRNGQLR
                     ATAAGTRSSYSSIARDYNGTYSAQRQELVESFEGYAVLQDAFVAAISRPIYREWLKMA
                     IASQAIKIPSDIDERSLFNAVYSGPVMPWIDPIKEANAWKERIKGGLATEGQAIRASG
                     NNPAEVKRQRIVEIRENQAEGLKFDTDLTNYSQQNSGSSNRNLEDSTKENEKE"
     gene            4582..>5673
                     /locus_tag="PWX02_002001"
     CDS             4582..>5673
                     /locus_tag="PWX02_002001"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729979.1"
                     /translation="MTTVVLDTVKVVANPPVIKPIFPPEFSEMEKLKRENYYFGRRIA
                     YAPNKVQSYVSLANSSVELSKKINELEKKIISAKSIGEIFKIISGLLMLYAKILNKVE
                     QEKLKKLYSLLVSQYDEVNKIKKAYLSKKEIAEKAEKSNLKKFALTKVNPRDYDLILS
                     VSGGSRLWWLHVGKHKADEVNEAQKLIDALIILNETLTEFSNESHRLVTTLSRHIVTN
                     IKQTPKLPITSRNRNTPVTSFDMLYYYRAKTGKSVTLKQLGLFNTVRELVKKPNALGK
                     KDNRSVHGDFIQQIITKNARSFKNTYSFKEGIWNINNALWAIGSAIVEGNFTGSAVQK
                     GDRFYLEGSIDYSFYDKFTDPYDIFNIIPG"
BASE COUNT         1715 a         1256 c         1369 g         1333 t
ORIGIN      
        1 ccgctaatga gagtgggagc ttaattgctc ccttttcatt tgcgttacat tacttagtta
       61 tgcttttgat tgtatcaaga tcataactaa gcaatgtgtt gcatagtttg taaaaggtac
      121 tcccgagggg ataccccttt ccacggggtt gcggcagcgc ggttttcggc agttttttgg
      181 gtttctaggc atcatcatct tcctgtgatg ttgccgtttt tttaggcggt tttgattttt
      241 ttaggatttg agcaaatgga taacttattt gatctaaaac ttaacatcaa tcagatcgcc
      301 gaactcaccg gattgcaccg ccagacagta tctcagcgag tggcagggct caccccatcg
      361 cttggcagta acagcaaatt aaagctctat gcgctgcgtg atttaatcct caccgggttg
      421 gcggaaaaga tgtcggcgga tgtcgatagc ctctctccgg ttgatcgcaa gtcattttgg
      481 caagcggaaa acgaacgtct caaatatgag gagaaaacag gcgagctaat cccagcctcc
      541 gaagtggcgt ttgaaatggg tgcaatggca aaagcagtag tgcaaacact cgaaaccttg
      601 ccggatattt tggagcggga ttgtggattg cagccgaaag accttatccg ggtgcaacaa
      661 gtaattgatg atgtgcgaga tcagatggct ctccatatcc aacaagcccc aacgaccgaa
      721 cagggagatg actaatgttt gcaagtgcca aagaaatccg ccgtgatatg gcgaacgctg
      781 tcaaagcacc tcgccgaatg aaagtctccg atgccgtagc ggaatatatg cgtgtgccac
      841 ttggcggagg aaactcggtc aaatgggata aacacaccgc cgcctatatg ttagaaccga
      901 tggactgcct caacagccga gaatatgatg cggtgatttt tgtcgggccg gcacgaacgg
      961 gtaaaaccat cggcttgatt gacggctgga taacctactc aattatctgc gatccgtccg
     1021 atttcctgtt ggtgcaactt acccaagaga aagccagcga acatagccga aaacggcttg
     1081 accgcacatt ccgcggttcg cccgaaattg ccaagcggct aagcccacgc aaaaacgata
     1141 acaatgtcca cgacaagtat ttccgagcgg gcaacttgct caaaatcggc tggccgtcta
     1201 tcaatgtgct ttcttcatcg gactacaaat atgttgctct gactgactac gaccgctggc
     1261 ccgaagatgt ggacggtgaa ggtgatggtt tctcccttgc ctcaaagcgg acgaccacct
     1321 ttatgtcagc aggaatgact ttggtggaga gttcgcccgg taaagatatt gttgatctca
     1381 aacatatccc gaaaagcacc cacgaggctc caccaacaac aggcatttta agcctataca
     1441 atcgcggtga tcgccgtcga ttttactggc aatgcccttg ttgctcagag tattttgaac
     1501 catcaatggc aaatatggtt ggctaccgag aagaaagcga ttttgtcaaa gccagtgaaa
     1561 acgcacggct acaatgcccc cactgccaag ccttgattcc gcccgaactc aaacgagagc
     1621 taaacatcaa aggcgtatgg ctcaaagagg ggcaaaaaat tgatgcaagc ggtcaaatta
     1681 gtggcgaaag tcgcaaatcc cgtattgcct ccttttggct tgaagggcca gcggcagcct
     1741 accaaacgtg ggcgcagctc acctacaaac tgctcaatgc cgaacacgaa tatgaaatga
     1801 ctggcagtga ggaaaccctc aaagcggtaa caaataccga ctgggggtta ccttacttac
     1861 cacgatctgc attagaacaa cgccgttctg atgagttaat ggaacgccgt gaagaggttg
     1921 aagagaaaac cgtgccaccg caatgtcgct ttatcgtggc ggcagtcgat gtacagggcg
     1981 gcaaaaatcg ccgttttgtg gtgcagatgg ttggctatgg cgagaatggc gaacgctggt
     2041 tgattgaccg ctacaacatc tcgcacacac tacccgacag agatggtgtg atcgaaaaaa
     2101 tcgaccctcg tattcccgaa gattggcata ttctgatttc agatgtgctg gaaaaacgct
     2161 acgtccttgc tcacaagccg agcgacttta tgccgatttt ggcaatggcg gtggacagtg
     2221 gcggcgaaga gggcgtaacc gacaacgcct accaattttg gcgaaaatgc cgccgagatg
     2281 gctatgcgaa acgggtctat ttagtcaaag gtgacagcac caaacggcaa aagctcatca
     2341 ccaaaacgca ccccgacaac acctcacgga gcgaccgcca cgcctccgca cggggcgatg
     2401 tgccgttgta tctgctccaa accgactacc tcaaagatcg gattaacaac gccctttccc
     2461 gtgatacggt gggggcaaac tacatccact tcccagactg gctaggcgaa tggtttttca
     2521 atgaattgac ctacgaggag cgaggagccg acggcaaatg gcggaaaccg ggcaaaggca
     2581 ataacgaagc cttcgacctg ttctgctatg cccacgccat tgcaatttta cgaggctacg
     2641 agcgaatcaa atggggcgat gaaaacgatg tgccaagctg ggcaaaactg cccgacatca
     2701 accctaacat cattcgggaa acgccaagaa cggctgaaca agcggtcgaa attgaagaaa
     2761 aacccgcaaa accacaaccc aaaccgacca aagcaaaaag cagttggcta agtggtggcg
     2821 gacgaaaaac aggaggctgg ctatgagcct ttacaccatc gatgaactca aagacaaaat
     2881 ccgcacgctt gatgagaaaa ttgagaccgc tcaatcgcag gtgagcttta acgggcggtc
     2941 ggtatcctat caagtggcgg aattaaccaa acagcgtgat cgctatcaaa caatgctgga
     3001 tgaacaactt gcccaaagcg ggcaacacac caaacagcat cgaattaaat atgcgaggtt
     3061 tatttgatga acttcctcga aaaaaccatt gccacccttt ccccgaaatg ggcggcaaat
     3121 cgctctcgaa atcgctatgt gctaaatgcc tatgaggcgg cacagcctag ccgaacccat
     3181 aaggcaagcc gagagagcaa aggggcaaac tctactgttc gccaaagtgc ggtcagccta
     3241 cgggaacagg cacgggcgtt agaccaaaat cacgatattg tgatcggcat tttagacaaa
     3301 atggaagaac gggtgattgg ctcgaaaggt attcatatcg aaccacagcc actcacgctt
     3361 gccggtgatg ttcacgaaga tctcgcagag caaatccgca agctgtgggc ggaatggtcg
     3421 gtgaaacccg aagtaacagg cttatatact cgccccctgt tagagcgaat gctactgcga
     3481 acttggctac gagacggtga agtgttcgtg cagttggtga aaggcaaagt ggcagggtta
     3541 gaacacggct cgccgattgc gttttcactc gaagccttag agccggactt tgtgccaatg
     3601 caatccgatg aggcaaaaaa cggcttggtg caaggcgtgt ttctcaatgc gtggcggaaa
     3661 cctaccgctt accaagtcta tttggataat ccgcaagaaa gtaacggaat gtacggcaaa
     3721 gtgaaaaccg tgtcggcaga aaatatgttg cacctcgcct ttcgtaagcg gttacatcaa
     3781 atccgtggcg tgagtatgtt gcacggcgtg attgtgcgct tggcagatct gaaagagtac
     3841 gaagagagcg aacgagtcgc cgcacgcatt gccgcagcga tgacgatgta cattaaaaaa
     3901 ggcgatgcgg cactgtatga tgacgacagt aacagcgatg gcaaccgcct gtttgatatt
     3961 gcccccggtg cagtgattga tgatttaaaa ccgggtgaag acattggctt aatcaattcc
     4021 aaccgcccaa atgtgaattt ggaaactttc cgcaatggac aactgcgagc cactgccgcc
     4081 ggtactcgtt cgagctattc gagcatcgcc cgagactaca acggcactta ctctgctcag
     4141 cgacaagaat tggttgaaag ttttgaaggc tatgcggtat tgcaagatgc gtttgtggcg
     4201 gcaatcagtc gcccgattta tcgggagtgg ctcaaaatgg cgattgcctc acaagctatc
     4261 aaaatcccgt ccgatattga tgaaagatcg ctgtttaatg ccgtctattc cggccctgta
     4321 atgccgtgga ttgatcccat caaagaggcg aatgcgtgga aagagcggat taaaggcggt
     4381 ctggcaaccg aagggcaagc gattcgagcc agtggcaata acccagccga agtcaaacgg
     4441 caacggattg tcgaaatccg agaaaaccaa gccgaagggc tgaaatttga taccgattta
     4501 actaattaca gccaacaaaa tagcggcagt tcgaatagaa atctcgaaga ttctactaaa
     4561 gaaaatgaaa aggagtaaat tatgacaaca gttgtgttag atacagttaa agttgtggca
     4621 aacccaccag taatcaagcc aatatttcca ccagaattca gtgaaatgga aaagctgaag
     4681 cgtgaaaact attactttgg tcgtagaatt gcttatgccc caaataaagt tcaaagttat
     4741 gtttctttag caaactcatc cgtagagtta agtaagaaaa tcaatgagtt ggaaaagaaa
     4801 attatttcgg ctaaatctat tggtgaaatt tttaagatta tcagtgggtt acttatgcta
     4861 tatgctaaaa ttctaaataa agttgagcag gagaagttaa aaaaactgta ttcgttactt
     4921 gtttcacagt atgacgaagt taataaaata aaaaaagcat atttatctaa aaaagagata
     4981 gcagaaaaag cagaaaaatc aaatcttaag aaattcgcct taacaaaggt aaatccgaga
     5041 gattatgatt tgattttatc tgtttcaggt ggttcaagat tgtggtggtt acatgttggt
     5101 aagcataaag cagatgaagt taatgaggcc cagaaattaa tagatgcatt gatcatactc
     5161 aatgaaacac ttactgagtt ttcaaatgaa agtcatcgac tggtaacaac tttatcaagg
     5221 catattgtta caaatataaa acaaacccca aaattaccga ttacatctcg aaatagaaat
     5281 actccagtca catcctttga tatgctttac tattatagag ctaagactgg caaatcagtt
     5341 actttaaaac aattaggatt atttaatact gtccgagagt tagtaaagaa accaaatgca
     5401 cttggtaaga aagataaccg tagcgtacat ggtgatttta ttcagcaaat cattactaaa
     5461 aatgctcgtt cttttaaaaa tacttactct tttaaagaag gtatttggaa tattaataat
     5521 gcattatggg caattggtag tgctattgtt gaaggcaatt ttacgggtag tgcggtacaa
     5581 aaaggagata gattttatct tgaaggtagt atagattata gcttttatga taagtttact
     5641 gatccatacg atatttttaa tatcattcct gga
//
LOCUS       DANJWC010000146         5600 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.146, whole genome shotgun sequence.
ACCESSION   DANJWC010000146 DANJWC010000000
VERSION     DANJWC010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.146"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..101
                     /locus_tag="PWX02_002002"
     CDS             <1..101
                     /locus_tag="PWX02_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL1729980.1"
                     /translation="REISEKHGMELTNHSLYLYGKCSNLTNCDEKK"
     gene            184..2850
                     /locus_tag="PWX02_002003"
     CDS             184..2850
                     /locus_tag="PWX02_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HDL1729981.1"
                     /translation="MTMEENTHTVVDAQIRQPRKISPFWLLPIVAFIIGALLFFQILK
                     EQGETITIRFSKGDGITAGKTAIRYQGLQIGQVKRVYFVDNLKEVEVQAEINPEAKSI
                     LRKQTKFWLVQPSASLAGVSGLDAIVSGNYITLLPGEGDFVDEFIAEEDPPAITVSDG
                     DLLIRLISDDIGSITVGASVYFRKVPVGNIADYRFTKDQKKIEIDVVINQKYAHLVKK
                     ESRFWNISGISADVNLQGIKINMDSLASVVQGAVAFDSPDNMEIAEQGQKFELYPDLK
                     SAKRGIEINVKLPLTHNLKINETGVFHQNVQIGMLSQLKLPQVKDNGVPQQESGQHKQ
                     IDGVLLIDPSYENLLRSQTKILLKEPKFSLNTEQLTKVGELLRGVYFEVHPGEGEPQS
                     EFVVQNEADYLLSLPNLLSFSLIAPQAYGVDTNQGIYYSDVQIGEIVKRSLELDKVRF
                     DVIIYPPYRNLIGSNSKFVAISNIDMSVGLDGLRVHAGSPSEWLKGGVRLINGKNDGS
                     PKKQYALYKDIESAESGMATLDKKATTTLSADSLSGISEGSLVLYRDFQVGEVLKITP
                     KQQKFEVELFIQPNYRHLLTEKSRFWIEPATSVDVSLKGVNITTAPLMRTLKGAISFD
                     NGGSKGNKTLYASKEKATSGNTYLTLIAKDASKLSEGMDIKYMGLTVGQIEKLELQNA
                     KKQIKATAYIQRKYYPLIAKAGSKFNVISPEISTSGFKNLDAALQNYITVDVGSGQAQ
                     NQFVLADTDTVKTTYANGFPIIVETTNANGIQPEAPVLYRGMQVGIVSHLSLSEVNDR
                     VLIHLKINSKYQHLVRTNTQFWQASGYTMDVSLQGVSMNSGTMSQLLNGGIEFSTPYT
                     KVVKPQAQPNRRFFLQRKLPDEAPAWDQGIAE"
     gene            2868..3377
                     /gene="luxS"
                     /locus_tag="PWX02_002004"
     CDS             2868..3377
                     /gene="luxS"
                     /locus_tag="PWX02_002004"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDL1729982.1"
                     /translation="MPLLDSFKVDHTKMNAPAVRVAKTMTTPKGDTITVFDLRFCRPN
                     IDILPVRGIHTMEHLFAGFMRDHLNSEQVEIIDISPMGCRTGFYMSLIGTPSAEEVVK
                     AWTASMEDALDKIPDVSAIPELNEYQCGSYKEHSLEEAHQIARNVLAAGIGVNRNEDL
                     ALDEKLLNP"
     gene            3445..5292
                     /locus_tag="PWX02_002005"
     CDS             3445..5292
                     /locus_tag="PWX02_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1729983.1"
                     /translation="MFEKLFQWFEARVETYPNETPRTPKSGLIPFIYDATKGMRFYIL
                     LLTVLVAAVGIIEAVLFRFMGDLVDWVNKYSPTELWAEKGWTIIGMFFIALLAVVFVY
                     LASSIRFQSLQGVFPMRLRWNFHRLMLGQSLGFYQDEFAGRVSAKVMQTALSVRDVIM
                     TCADMLVYVLVYFTTSSVILLQLDGWLFVPFILWVISLAATIIYFVPKLAQAAQEQSD
                     ARSLMTGRITDAYANIATVKLFSHGNRESAYAKESMEEFMVTVHKQMRLVTVIETVTN
                     LTSMALIISTAGIGLWLWGSSVVTAGAIATSTALALRIKGLSQWIMWEFARLFENLGT
                     VQDGMITLSKPHNVVDKIDAKPLNVTKGEIKFENVDFAYDPKKPLLKDFDLTIKPGEK
                     VGLVGRSGAGKSTLTNLLLRFYDIQDGSITIDGQNIRDITQESLRSQIGLVTQDTSLL
                     HRSVRENLMYGRPNATEEEMIKAVEQAAASEFIPNLQDAKGRTGFDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEVAIQENLTQLMEGKTVVAIAHRLST
                     IMAMDRLIVLDKGEIVEQGTHEELLAQNGVYARLWAHQSGGFLPENTDI"
     gene            5479..>5600
                     /locus_tag="PWX02_002006"
     CDS             5479..>5600
                     /locus_tag="PWX02_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729984.1"
                     /translation="MEQIVSSLWSDCLNHLQTKVSPTDYSTWLRPLQASSENGEL"
BASE COUNT         1691 a         1125 c         1212 g         1572 t
ORIGIN      
        1 aacgtgaaat cagtgaaaaa cacggaatgg aattgactaa ccacagttta tatctgtatg
       61 gtaaatgcag taatttaacc aactgtgatg agaaaaaata atatttctta ttttgactta
      121 tttctattac cgaagtccct ttgggtcttc ggtattgttc tatataggtt tccctaattt
      181 aaaatgacga tggaagaaaa tacccacact gttgtagatg cccaaatccg gcagcctcgc
      241 aaaatttctc ctttttggct gttaccaata gttgctttta ttattggtgc tttgctattt
      301 ttccaaattt taaaagagca aggtgaaaca atcacgattc gatttagtaa aggtgatggc
      361 attactgccg gaaaaaccgc tattcgttac caaggcttgc agattggtca ggttaagcgt
      421 gtttattttg tcgataattt aaaagaagtg gaagtgcaag cagaaataaa ccctgaagcc
      481 aaaagtattt tacgcaaaca gactaaattt tggttagtgc aaccaagtgc ttctttggct
      541 ggcgtttcag gcttagatgc gattgtttct ggtaactata ttaccctact tcccggtgaa
      601 ggcgatttcg ttgatgaatt tattgcagaa gaagatcctc cggctattac cgtgagcgat
      661 ggcgatttac tgatccgttt aatctctgat gacataggct ctattactgt cggagcgagt
      721 gtttatttcc gtaaagtgcc agtgggtaat attgccgatt atcgttttac caaagaccag
      781 aaaaaaatcg aaattgatgt cgtcattaat caaaaatacg ctcatttagt gaaaaaagag
      841 agccgatttt ggaatattag cggtattagc gctgatgtaa atttacaagg tattaaaatc
      901 aatatggaca gccttgcctc ggtagtacaa ggtgcggttg cttttgattc gcctgataat
      961 atggaaattg ccgagcaagg acagaaattt gagctatatc cggatttgaa atcagctaaa
     1021 cgtggaattg aaatcaacgt aaaactaccg cttacacata atttaaaaat taatgagact
     1081 ggtgtttttc accaaaatgt gcaaattggt atgctttcgc agctaaaatt accgcaagta
     1141 aaagacaacg gtgtgccaca gcaagaatca gggcagcata aacagattga tggggtttta
     1201 ttaattgacc caagttatga aaatttacta cgctcccaaa ctaaaatctt gctcaaagag
     1261 ccgaaattct ccttaaacac agagcagctg acgaaagtag gtgagctgct gcgtggtgtc
     1321 tattttgagg ttcacccggg ggaaggagaa cctcaatcgg aatttgtggt acaaaacgaa
     1381 gccgattatc tattaagttt accgaattta cttagcttta gcttaattgc tcctcaagcc
     1441 tatggggtag ataccaatca ggggatttac tatagcgatg tgcaaatcgg agaaatcgtc
     1501 aaacgtagcc tagagttaga caaagtacgt tttgatgtca ttatttatcc gccttatcgc
     1561 aacctgattg ggagtaacag caaatttgtt gcgatatcca acattgatat gtcggtgggg
     1621 ctagatgggt taagagttca cgccggttca ccatctgaat ggttaaaagg cggtgttcgc
     1681 ttaattaacg gtaaaaatga cggtagcccg aaaaaacaat atgcacttta taaagatata
     1741 gaaagtgcgg aatcaggcat ggctacctta gataaaaaag ccaccactac cctttcggca
     1801 gattcattat ctggtatcag cgaaggctct ttggtgctat atcgtgattt tcaggtcggc
     1861 gaggtgttaa aaataacccc gaaacaacaa aaatttgagg ttgagctatt tatccagcca
     1921 aattatcgtc atctattaac cgagaaaagc cgtttctgga ttgagccggc aacgagtgtt
     1981 gatgtgtcac tcaaaggcgt gaatatcact acagctccat taatgcgtac cttaaaaggg
     2041 gcgatcagct ttgataatgg cggttcaaaa ggcaataaaa cgctttatgc aagtaaggag
     2101 aaagccactt cgggcaatac ttatctcacg ctgattgcaa aagatgcgag taagttaagc
     2161 gaaggaatgg acattaaata tatgggctta accgtaggtc aaattgaaaa acttgagcta
     2221 caaaatgcaa aaaaacagat aaaagcgacc gcttacattc aaaggaaata ctatccgtta
     2281 attgcaaaag ccggcagtaa atttaatgtt atctcgcctg aaatcagtac ctccggcttt
     2341 aaaaatttag atgcagcact gcaaaattac attacggttg atgtcggttc agggcaagct
     2401 caaaaccaat ttgttcttgc agataccgat acggtaaaaa ccacctatgc caacggtttc
     2461 ccgattattg ttgaaaccac taatgccaat ggtattcagc cagaagcacc tgtgctttat
     2521 cgtggaatgc aggtggggat tgtcagccat ttaagcctca gcgaagtaaa tgatagagtt
     2581 ttaattcacc tgaaaattaa tagcaaatat cagcacttag tcagaaccaa tacccaattc
     2641 tggcaagctt caggttacac gatggacgtg agcctgcaag gtgtaagtat gaactcaggc
     2701 acaatgtcgc agttattaaa cggcggtatt gagttctcta ccccttacac taaagtggtg
     2761 aagcctcaag ctcagccaaa cagacgattt ttcttacaac gcaaactacc tgatgaagcc
     2821 cctgcttggg atcaaggaat tgcggaataa tattgatgga gtaaaaaatg cctttattag
     2881 atagttttaa agtagatcac accaaaatga acgccccagc cgtgcgtgtt gcaaaaacaa
     2941 tgaccacacc gaaaggcgat accattaccg tgttcgatct ccgtttctgc cgcccgaata
     3001 ttgatattct accggtgcgt gggattcaca caatggaaca tctttttgcc ggctttatgc
     3061 gtgaccacct caatagcgaa caagtggaga tcattgatat ttcgccaatg ggctgccgta
     3121 ccggttttta tatgtcgctc atcggcacgc cttcagcaga agaggttgtc aaagcgtgga
     3181 cggcttcaat ggaagatgcg ttagataaaa tccctgatgt atcggcaatt cctgagctga
     3241 acgaatatca atgtggctct tacaaagagc attctttaga agaagctcac caaattgctc
     3301 gtaatgtgtt agctgccggc atcggtgtaa atcgcaacga agatttagcc ttagacgaaa
     3361 aattacttaa tccataattt tttaacatct tttcgggggc aaatggttat ttgcccccgc
     3421 attttattcg gaaatcacaa atttatgttt gaaaaactgt ttcaatggtt tgaggcaagg
     3481 gtggaaactt acccaaatga aacgccaaga accccaaaat caggtctcat tccttttatt
     3541 tacgatgcca ctaaaggaat gcggttttat atcttactac tgaccgtatt agttgcagct
     3601 gtcggtatta tcgaagcggt tctgtttcgc tttatgggcg atttagtcga ttgggtcaat
     3661 aaatactcgc caacagaact ttgggcagaa aaaggctgga cgattatcgg tatgtttttt
     3721 attgccctac ttgcagtggt gtttgtttac cttgccagca gcattcgctt tcaatctctg
     3781 caaggggtat tcccaatgcg tttgcgttgg aacttccacc gcttaatgct tgggcaaagt
     3841 ttaggtttct atcaagacga atttgcagga agagtgtctg caaaagtaat gcaaaccgcc
     3901 ctttccgtgc gtgatgtgat tatgacttgt gccgatatgt tggtgtatgt gctggtttac
     3961 ttcaccacct ccagcgtgat tttattacaa cttgacggct ggttattcgt gccgtttatt
     4021 ttatgggtca tctcattagc ggcaaccatt atctactttg tcccgaaatt agcccaagct
     4081 gctcaagagc aatcggatgc cagaagttta atgaccggtc gaattaccga tgcttacgct
     4141 aacattgcca ccgtcaaact cttctcgcac ggcaaccgtg aatctgccta tgcgaaagag
     4201 tcgatggaag aatttatggt gacagtgcat aaacagatgc gtttagtgac tgtaattgaa
     4261 accgtaacta atctaacctc gatggcatta ataatttcaa ctgcgggtat cggtttatgg
     4321 ctttggggtt caagcgtagt aactgcaggg gcgattgcca cctcaaccgc tttggcttta
     4381 cgcattaaag gcttatcaca atggattatg tgggaatttg ctcgtctatt tgaaaattta
     4441 ggtacggtac aagacggtat gattacgctt tccaaaccac acaatgtggt agataaaatc
     4501 gatgcgaaac cgttaaatgt aaccaaaggc gaaattaaat ttgagaatgt ggattttgcc
     4561 tacgatccaa aaaaaccgct actgaaagat tttgacctca ctataaaacc gggggaaaaa
     4621 gtcggtttag tcgggcgtag tggtgcaggt aaatcaacgc tcaccaattt attattgcgt
     4681 ttttacgata ttcaagacgg ttcaatcacg attgacgggc aaaatatccg tgatattacc
     4741 caagaaagcc ttcgctcaca aattggtttg gtgactcaag acacctcgct gttacatcgc
     4801 tctgtgcgtg aaaacttgat gtacggtcgc ccgaacgcca ccgaagaaga gatgatcaaa
     4861 gccgttgaac aagctgccgc tagcgaattt attccaaacc tgcaagatgc caaaggcaga
     4921 accggttttg atgcacacgt tggcgaacga ggggtaaaac tttccggcgg acaacgccaa
     4981 cggattgcga ttgcccgagt gatgctaaaa gatgctccga ttttattact cgatgaggca
     5041 accagtgcgt tagactctga agtagaagtg gcaattcaag aaaatctcac gcaattaatg
     5101 gaaggtaaaa ccgtggttgc gattgcccac cgcctctcta ccattatggc aatggacaga
     5161 cttatcgtgt tagataaagg cgaaatcgtt gaacaaggca cgcacgaaga attgcttgct
     5221 caaaacggtg tttacgccag actttgggct caccaaagcg gggggttctt accggagaat
     5281 acggatattt aatcaactcc atcacacatc atactaagca agcggtcatt ttttgttagc
     5341 aatttgcaag atttcaacaa aaaacgaccg cttgttcgcc aattaaaacc gcttccaaac
     5401 accaataaaa tctatcaaaa atttgcaaga tttctcccct tttttcaggt atcattatga
     5461 cctttaataa caataatttt ggagcaaatt gtgtcttccc tttggtctga ttgccttaat
     5521 cacttacaaa cgaaagtctc accaaccgat tacagcactt ggctgcgtcc tctgcaagcg
     5581 agttctgaga atggggaact
//
LOCUS       DANJWC010000147         5540 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.147, whole genome shotgun sequence.
ACCESSION   DANJWC010000147 DANJWC010000000
VERSION     DANJWC010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.147"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..1320)
                     /gene="ubiB"
                     /locus_tag="PWX02_002007"
     CDS             complement(<1..1320)
                     /gene="ubiB"
                     /locus_tag="PWX02_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HDL1729985.1"
                     /translation="MNIKNLNRFYHIIRTFLRYGIDEAIPNLPITRKIKLGRKALFWV
                     KNQHSDKPYGVRLRLALQELGPVWIKLGQMLATRRDLFAPELADQLALLQDNVEPFDG
                     KIARQIIEQALGGKLETWFENFDETALASASIAQVHTAKFNQNQPLAGKDVVLKVIRP
                     GIEPVIKADLELMYEIAHFIPKLSNDGKRLRAVEVVREYEKTILDELDLRKEMHNAIR
                     LRANFENSEMLYVPEMYQDFCHKNLIAMERIYGIPVSNIAELEANSTDMKLLAERGVQ
                     VFFTQVFRDSFFHADMHAGNIFVNREHPENPQYIGIDCGIVGTLNHNDKRYLAESFVA
                     FFNRDYRRVALMHVESGWTPPDTDIDKFEQAFREVCEPIFAKPLSEISFGHVLLNLFN
                     VAREFNMEVQPQLVLLQKTLLYIEGLGRQVYPQLDLWQTAKPFLQNWL"
     gene            complement(1394..2371)
                     /locus_tag="PWX02_002008"
     CDS             complement(1394..2371)
                     /locus_tag="PWX02_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDL1729986.1"
                     /translation="MKQIIAQARENAVRAVDFQRVLMYWHIGKRIFEQEQQGQERADY
                     GTYLIKMLAKELEPEFGSGFCSRQLERYRQFYRAFPIATALRSQLNWTQYRRLIQISD
                     SDKREFYLAESCKNNWSSRQLERQINSSLYERLLLSNDKESVLAVARNEQQPVDPKQL
                     IKDPMVLEFLGLKRESAYYEKDLEQAIITHLQDFLLELGNGFSFIARQKRLHLDGDDF
                     FVDLVCYNRLLQCFVIFEIKTHKLTHQDLGQLQMYVNYFDRVEKLPHENATIGVLLCA
                     DKNDAVVKFSLPENQNKIFASQYQLYLPSEQTLIAEVQKEIENFEQKYP"
     gene            complement(2532..3179)
                     /locus_tag="PWX02_002009"
     CDS             complement(2532..3179)
                     /locus_tag="PWX02_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HDL1729987.1"
                     /translation="MLNNVKQQLMLPQFTFGLIETAFNALLKRSPHIEPNLRKLSGKV
                     LKIALTSPKIDFFVLFSESRTDWLGNYEGEADCTVQLAFDTLPKLADKQKLTELINNK
                     SLVLTGDIQVLQHFTALLEQLEKDPAELLSPFVGDVVAQTSTDLAKKLLGKVKAQFEQ
                     NNQHLVENLIQERPVLVHRLQAVHFYDQVEELAQQALKLEQKFTHYVRSSSHQQS"
     gene            complement(3370..4158)
                     /gene="ubiE"
                     /locus_tag="PWX02_002010"
     CDS             complement(3370..4158)
                     /gene="ubiE"
                     /locus_tag="PWX02_002010"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HDL1729988.1"
                     /translation="MSDVEQLAPKDEVQQSAETTHFGFKTVAKEEKQQLVANVFHSVA
                     GKYDLMNDLLSFGIHRVWKRFTIDCSGVRKGQKVLDLAGGTGDFTAKFSRIVGESGEV
                     ILADINSSMLEVGREKLRNLGVVGNVSYVQANAECLPFADNTFDCIVISFGLRNVTDK
                     DKALRSMFRVLKPGGRLLVLEFSKPIIDPISQLYNFYSFNILPKVGEVVVNDAESYRY
                     LAESIRMHPKQDELKAMMEQTGFESVNYYNLSAGIVALHRGYKF"
     gene            4455..5408
                     /gene="corA"
                     /locus_tag="PWX02_002011"
     CDS             4455..5408
                     /gene="corA"
                     /locus_tag="PWX02_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDL1729989.1"
                     /translation="MIRAFGLDNACLISVDETNPTLLNDAIWIDLIDPSEAERTVLEN
                     RLDQTLAEERELEDLEASARFFEDEDGLHLHSFFYCLDDDDYADIATVAFTIRDGRLF
                     TLRDRDLPAFRLYRMRSRREKLIDSNAYELLLDLFETKIEQLADVIEEVYADLEKLSR
                     VILNGQQENENFNDALSRLTEFEDASSKVRLCLMDTQRALSFLLRKTRLPNNQLEQAR
                     DIMRDIESLQPHNESLFQKVNFLMQAAMGYINIEQNRIMKFFSVVSVMFLPATLVAST
                     YGMNFEFMPELHFQYGYPMAIGLMIVAASAPYLYFKRKGWL"
     gene            complement(5434..>5540)
                     /locus_tag="PWX02_002012"
     CDS             complement(5434..>5540)
                     /locus_tag="PWX02_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HDL1729990.1"
                     /translation="HFELYLKECEWRFNHSDLKSQISFLKQLVKGSLG"
BASE COUNT         1551 a         1225 c         1075 g         1689 t
ORIGIN      
        1 cagccagttt tgtaaaaacg gttttgcggt ttgccataaa tccaactgtg gataaacctg
       61 cctgcccaaa ccttcaatat aaagaagtgt tttttgcaac aacaccaact gcggctgcac
      121 ttccatatta aattctcttg ccacattaaa caggttgagc aaaacgtgtc cgaatgaaat
      181 ttccgataac ggcttagcaa aaatcggctc acatacctca cgaaaggctt gctcaaattt
      241 atcaatatcg gtatccggcg gcgtccagcc cgattccacg tgcattaagg caacacggcg
      301 gtaatctcgg ttgaaaaatg cgacaaaact ttcggctaaa tagcgtttgt cattgtggtt
      361 caacgtgccg acaatcccgc agtcaatgcc gatatattgc ggattttccg ggtgttctct
      421 gttcacaaaa atattgccgg cgtgcatatc ggcgtggaaa aaactgtcac ggaatacttg
      481 ggtaaaaaat acctgcacac cacgctctgc cagcaatttc atatcggtgc tgttcgcttc
      541 caactcggca atgttagaca ctggaatgcc ataaatccgc tccatcgcaa tcaagttttt
      601 atggcaaaaa tcttgataca tttcaggcac atacagcatt tcgctatttt caaaattcgc
      661 ccgaagccga atggcattgt gcatttcttt gcgtaaatcc agctcgtcta aaatagtttt
      721 ttcgtactct cgcaccactt caaccgcccg tagccgtttg ccgtcattcg ataatttcgg
      781 gataaagtga gcaatttcat acatcaactc taaatccgcc ttaatcaccg gctcaatgcc
      841 cgggcgaatc acttttaaca ccacatcttt gcctgcaagc ggttgatttt ggttaaattt
      901 tgccgtgtga acctgtgcaa tcgaagcaga agctaaggca gtttcatcaa agttttcaaa
      961 ccaagtttca agctttccgc ccaaagcctg ctcgataatt tgacgagcga ttttgccgtc
     1021 aaacggttca acattatctt gtagcaaggc gagctggtcg gcaagctccg gtgcaaacag
     1081 atcacgtctg gtagctaaca tttgcccgag cttgatccaa accggtccaa gctcttgcaa
     1141 agccaaacgt aaacgtacac cgtaaggttt gtctgaatgt tgattcttca cccaaaacaa
     1201 agccttacgc cctagcttta ttttgcgggt aattggcaga ttcggaatgg cttcatcaat
     1261 accatagcga agaaaagtgc ggataatatg gtaaaagcgg ttgagatttt tgatgttcat
     1321 tgaaatatat atttaatggt aattaggaag attttagcaa aattctgtaa aaatttaacc
     1381 gcttgttggg ctactatggg tatttctgct caaaattctc aatttctttc tgtacttccg
     1441 caataagtgt ttgttcagag gggaggtaaa gttggtattg gctggcaaaa attttattct
     1501 gattttccgg tagcgaaaat tttaccaccg catcattttt atcggcacaa agcaagacac
     1561 caatagtggc attttcgtgc ggcagttttt caactcggtc aaaataattg acgtacatct
     1621 gtaattgccc caaatcttga tgggtgagct tgtgggtttt aatttcaaaa atcacaaaac
     1681 attgaagtaa acggttgtaa caaaccaaat cgacaaaaaa atcatcaccg tctaggtgca
     1741 aacgcttttg gcgtgcgata aacgagaaac cgttacccaa ttctagtaga aaatcctgca
     1801 aatgcgtaat gatggcttgc tctaaatcct tttcgtaata agcactttcc cgttttagtc
     1861 cgagaaattc caataccatc gggtctttaa tgagctgttt cggatcaacc ggctgctgct
     1921 catttctggc aacagcaagc acactttcct tatcattact gagcaataat cgctcgtata
     1981 gactactatt aatttgccgt tccagctggc gtgaactcca attattctta caactttcag
     2041 ccaaataaaa ttcccttttg tcagaatcgg agatttgaat tagacgacga tattgtgtcc
     2101 aattcaattg cgaccgcagt gcggtcgcaa ttggaaacgc acgataaaac tggcgataac
     2161 gctctaattg acggctacaa aaaccacttc caaattctgg ctcaagctct tttgcgagca
     2221 ttttaatcag gtatgttcca taatcagcac gctcttgccc ttgttgctcc tgttcaaaaa
     2281 tccgttttcc gatgtgccaa tacatcagca cacgttggaa atctaccgca cgaaccgcat
     2341 tttcacgagc ttgagcaata atttgtttaa tatcttagat catcgttgtt tctattaaca
     2401 taaaagcctc cgttttggat taagtttatg cttaatctat ccggaggcaa tttctttcac
     2461 tcaacttttc gagtcatatc acaattttat tttgttaaat ttaattttgc acaaacgctt
     2521 gcgtttgaac atcagctttg ctgatggctc gaagagcgaa cgtagtgagt aaatttttgc
     2581 tctaatttga gcgcttgttg ggcaagttct tcgacttggt catagaaatg caccgcctgt
     2641 aaacgatgca ccaacaccgg tctttcttgg attaaattct caaccaaatg ttgattattt
     2701 tgctcaaatt gagcttttac tttacctagc aattttttag caagatcggt gctagtttga
     2761 gccactacat cgccaacaaa aggggaaagc agttcggcag ggtctttttc gagctgttca
     2821 agtaatgcag tgaaatgttg caacacttga atatcgcccg tcagcaccaa cgatttatta
     2881 ttaatcagct cggttaattt ttgcttatcc gctagtttcg gcagggtgtc aaatgccagt
     2941 tgcaccgtac aatctgcttc cccctcgtag ttgccaagcc aatcggttcg gctttcgcta
     3001 aacaacacaa aaaagtcgat tttaggcgaa gttagggcga tttttagcac ttttccggac
     3061 aatttacgca aattcggttc aatatgaggc gagcgtttaa gtaacgcatt aaaagcggtt
     3121 tcaatcagac caaaggtaaa ttgcgggagc attaattgtt gttttacatt atttaacatt
     3181 cgtacctact taccctcttc acttttggga gaaggataga ttgttcttca ttaagaagca
     3241 aaatcaggga gaggggtatt tgcaaaatat tgaataaata tgaccgcttg ccccctctct
     3301 ctgcttattt cgctaaacgc tcataagcgg tctctctccc acaaagggag agagtcattt
     3361 aagagaaaac taaaatttat aacctctgtg taatgccaca atgccggcac ttaagttgta
     3421 gtaattcacg ctttcaaagc cagtttgttc catcattgct tttagctcgt cttgtttcgg
     3481 gtgcattcgg atcgattcag ctaaatagcg gtaactttcg gcgtcattta ccaccacttc
     3541 gcccactttc ggcaaaatat tgaatgagta gaaattataa agctggctga ttgggtcgat
     3601 aatcggtttt gagaattcca acacaagtaa gcgaccgccc ggttttagca cgcggaacat
     3661 tgaacgtagg gctttgtctt tgtcggtaac attacgcaag ccaaaactaa tcacaataca
     3721 atcaaaagtg ttatcagcaa aaggcaagca ttcggcattc gcttgaacgt agctcacatt
     3781 tccaaccacg cctaaattgc gtaatttttc ccgcccgact tccaacattg agctgttaat
     3841 atcggctaaa atcacctcac cactttcacc aacaatgcga gagaattttg cggtaaaatc
     3901 acccgtgccg ccggcaagat ccagcacttt ttgcccttta cgcacgccac tgcaatcaat
     3961 ggtaaagcgt ttccacactc gatgaatgcc gaaagaaagc aaatcgttca ttaggtcata
     4021 tttgcctgcc accgagtgaa acacatttgc gaccagttgc tgtttttctt ctttcgctac
     4081 ggttttaaac ccaaagtggg tggtttctgc cgattgttgc acttcatctt taggtgctaa
     4141 ttgttctaca tcgctcattt tcttgtctct agttgcaaaa tttaggcgta accatagcaa
     4201 aaaagcggtc gtttttcttt aattttttgc aaattttctt aagtaaataa ccgctttctt
     4261 gactagaaaa gccaaattct gtaacattcg cacactattt tttcttgagg attacacatt
     4321 ttggacagtc atagtcgata cttacccgtt taacctatct tgtctaacct caatgttacc
     4381 gacaaggtcg gtaatgctcg cctagtatct ttgttccttt ttgttccaag cctcctaact
     4441 atttggagct aaacatgatc cgtgcttttg gactagataa cgcatgttta atcagcgttg
     4501 atgaaactaa cccaacttta ttaaatgatg cgatttggat tgatctcatc gatccatcag
     4561 aagctgaacg aaccgtatta gaaaaccgct tagaccaaac actcgctgaa gaacgtgaat
     4621 tggaagactt agaggcgtct gcccgtttct ttgaagatga agacggtttg cacctgcact
     4681 cgttctttta ctgcttagat gatgacgatt acgccgatat tgccactgtc gccttcacta
     4741 ttcgtgacgg gcgtttgttt accttgcgtg atcgggattt acccgcattt cgcttgtatc
     4801 gaatgcgttc ccgccgtgag aaattgattg acagcaacgc ctatgaactc ttactcgatt
     4861 tatttgagac caaaatcgag cagcttgcag acgtaattga agaagtctat gctgacttag
     4921 aaaaattaag tcgggttatt ctaaacggtc agcaagaaaa tgagaatttc aatgatgccc
     4981 tttctcgctt aactgaattt gaagatgcaa gttcaaaagt gcgtttatgt ttgatggata
     5041 cgcaacgggc gttaagtttc ttactgcgta aaacccgttt gccgaataat cagctagaac
     5101 aagcccgtga cattatgcga gatattgaat ctctgcaacc gcacaatgaa tccttgttcc
     5161 aaaaagtgaa ttttttaatg caagcggcaa tgggttatat caatattgag cagaaccgaa
     5221 ttatgaaatt cttctcggtg gtgtcagtga tgttcctacc tgctacgctg gttgcttcta
     5281 cttacggtat gaactttgaa tttatgccgg aacttcattt ccaatatggc taccctatgg
     5341 cgattggttt aatgattgtt gccgcctccg ccccttatct ttattttaaa cgtaaaggtt
     5401 ggttataatt tattttgggg ctgtcctaga taactaacct aaactaccct taaccaattg
     5461 ttttaaaaag gatatttgag attttaagtc actgtgatta aaacgccatt cgcattcttt
     5521 caaatacagc tcaaaatgca
//
LOCUS       DANJWC010000148         5512 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.148, whole genome shotgun sequence.
ACCESSION   DANJWC010000148 DANJWC010000000
VERSION     DANJWC010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5512
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.148"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..512
                     /locus_tag="PWX02_002013"
     CDS             <1..512
                     /locus_tag="PWX02_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDL1729991.1"
                     /translation="GHHTNRKIGSPITDKGLQLLKCYQKDQSNTELVYFSRLNNGNLV
                     KSTTHIIIGHEGKPIGLFCINLNLSTPLDKTVSSFMNYDPIPPHYKSENFITHPKELI
                     EETFLKAVTHIEQHTTKHSKLYKKSIIFYLFENGIFELKEAVPIVAEKLSLTSHAIYK
                     HLREFKNNT"
     gene            541..1819
                     /locus_tag="PWX02_002014"
                     /pseudo
     CDS             541..1819
                     /locus_tag="PWX02_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249977.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1833..3134
                     /locus_tag="PWX02_002015"
     CDS             1833..3134
                     /locus_tag="PWX02_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDL1729992.1"
                     /translation="MNIHCEKLVQSILHAVNHEVVPALGCTEPISLALASAIARQYLG
                     HLPERIEAKVSPNLMKNGMGVAVPGTATVGLPMAAAIGAIGGEPDGGLEVLKNITPEQ
                     VKQAKAMLNTDKVSVSIFETEHILYSEATLFHQANRVCVRIAEHHTNVIYIEKNGQTI
                     LSKPCAVNNDNTTEIFASLSAKDIFNFSMQIELEKIRFIEQAAQLNSALSQEGLRADY
                     GLHIGRTLQKQIGLGLISDDLLNRIVIETTSASDARMGGASLPAMSNSGSGNQGITAT
                     MPIVVVARHLNVTDEQRIRALFLSHLMAIYIHSKLPKLSALCAVTTAAMGSCAGIAWL
                     LTGKFENISMAISSMIGDISGIICDGASNSCAMKVSTSVTSSYKSILMSMDNSQVTGN
                     EGIVEHDIDRSINNLCSIASRSMQYTDRQVIEIMVNKPKLK"
     gene            complement(3239..5305)
                     /gene="glyS"
                     /locus_tag="PWX02_002016"
     CDS             complement(3239..5305)
                     /gene="glyS"
                     /locus_tag="PWX02_002016"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDL1729993.1"
                     /translation="MTTQNFLAEIGTEELPPKALKKLATAFAENVENELNQAGLSFEK
                     VEWFAAPRRLAVKVLSLAESQPSKEVEKRGPAVSAAFDAEGKPTKAAEGWAKGCGITV
                     EQAERIATDKGEWLVHRAVIEGQPTKNLLVDIIAKSLANLPIPKTMRWGDKTEQFVRP
                     VHTVTLLFGAELIEGEILGVKSGRTIRGHRFLGEREFQIENADQYPQLLKDRGSVVAD
                     FNERKALILAKSQEKATALGGVADIEEDLLDEVTSLVEYPNVLTAKFEERFLAVPAEA
                     LVYTMKGDQKYFPIYSKDGKLLPHFIFVSNINPEDPSKIIEGNEKVVRPRLTDAEFFF
                     KTDLKQKLEDQLPRLETVLFQQQLGTLRDKTARIEALAGEIAKQIGADETKAKRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEDEEVAVALNEQYMPRFAGDNLPNSLVAC
                     SVALADKFDTLTGIFGIGQAPKGSADPFALRRAALGSLRIIVEKNLPLDLTDLVAKSA
                     QLFGDKLTNKDVVEDVVDFMLGRFRAWYESEGIAVDVIQAVLARRPTKPADFDARVRA
                     VSHFRTLDSAEALAAANKRVSNILAKADIAIGDIDVSACVEPAEKALAEAVIALKAEV
                     QPLIAQGDYTAVLDKLANLRQPVDAFFDGVMVNAEDQKLRQNRLAILSTLQGLFLQVA
                     DISLLQ"
     gene            complement(5314..>5512)
                     /locus_tag="PWX02_002017"
     CDS             complement(5314..>5512)
                     /locus_tag="PWX02_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1729994.1"
                     /translation="MNGKNDNARKEAEEHFNELQNGLLEAQDYKKDLDIKIRKLAREL
                     YNENIAQLEIEEIKEHLYKNW"
BASE COUNT         1506 a         1254 c         1092 g         1660 t
ORIGIN      
        1 acggacatca tactaaccga aaaataggtt cgcctattac agataaagga ttacagttac
       61 taaaatgtta ccaaaaagat caatctaata ctgagctggt ttacttttca cggttaaata
      121 acggtaactt agttaagtcc accacgcata ttattatcgg gcatgagggc aaacctatcg
      181 gattattttg tattaattta aatttatcga caccattaga taaaaccgtt agctctttta
      241 tgaattacga tcctattccg cctcattata aaagtgaaaa ctttattact cacccaaaag
      301 aacttattga agaaaccttt ctaaaagcag ttactcacat tgagcaacac actaccaaac
      361 attcaaaact ctataagaaa tccattatct tctatttgtt tgaaaatggc atctttgagc
      421 taaaagaggc agtacctatt gtggcagaaa aactctccct aaccagccac gcaatttata
      481 aacatttaag agaattcaaa aataacactt aattatactg ttacgattta aggagactct
      541 atggatactc aaaaactctc gcctgccgaa tttaaacaag caacaaaatt tgattctacc
      601 gacttcggct ggatcattat gagtattggt atggctatcg gggcaggcat cgtcttttta
      661 ccggtacaag taggacttat gggcatgtgg gtttttctac tttcttctat tatcggatac
      721 ccggcgatgt acctttttca gcgtctgttc attaatactt tagcagaatc tccggaatgc
      781 aaagattacc ccaccgttat tagtggctac ctgggcaaaa actggggaat ctttttaggt
      841 gtactttatt ttattatgtt agtgatttgg atgtttgttt attctaccgc aattacaaac
      901 gacagtgcct cctatcttct taccttcggc attaccaata cactactctc tgataatcca
      961 ttttatggct tagtgctaat ctgtatatta gttgccttat cttctcgagg cgaaaagcta
     1021 ctgtttaaaa tatctacatt tatggtatta actaagctat ttgtcgttgc cgccttaggg
     1081 cttgcaatga ttggaatgtg gcaactccat aatatcggtg caatgcctcc tttcggcaaa
     1141 cttataaaag atgccatcat tacccttcct ttcaccctaa cctctatact cttcttgcaa
     1201 actctcagcc caatggtaat ttcttaccgt gctaaagaac agtctcgtga agttgcgcgc
     1261 tataaagccc tacgagcgat gaatattgca tttgctatct tatttattac cgttttcttt
     1321 taagctgtct cttttacact tgcaatgggg agagaagaag cgctcaaagc atacgagcaa
     1381 aatgtttccg cattagcaat tgcagcccaa tttttccctg gtagttgggc aacctatgta
     1441 ggtgttgcat taaatatttt tgccgttatg acagcctttt tcggggtata ttttaggttt
     1501 tagagaagcc actcaaggca ttgtgatgaa cctattatta agaaaattcc cggctgaaaa
     1561 aatcaatcct aattatgtgc agaaagggat tatgttattt tccattctgc tggcttggag
     1621 tgcgattgta ttaaatgcac cggttttaag ctttacctca atctgtagtc ctatttttgg
     1681 cttagttggg tgcttgattc cggcctattt agtctataaa gtgcctgcat tattcaaata
     1741 taaagggcta gcactcaata ttattatcat taccggcatt ttattatgta tttctccatt
     1801 cctcacattt ttagagtaat aaggttttat ctatgaatat tcactgcgaa aaattagtac
     1861 aatccatttt acatgcagtc aaccatgaag ttgtccctgc attaggctgt acagaaccta
     1921 tttctctcgc ccttgcatcc gccattgcac gccaatattt aggtcattta ccagaacgta
     1981 ttgaggcaaa agtttcccct aacttgatga aaaatgggat gggagtagca gtaccgggaa
     2041 ctgcaaccgt cggattgcct atggcggcag ctattggggc aatcggaggg gagcctgatg
     2101 gtggattaga agtattaaaa aatattacgc cagaacaggt taagcaagct aaagctatgt
     2161 taaataccga taaagtgtct gtctctattt tcgaaacaga acatattcta tattctgaag
     2221 caacactatt tcaccaagcg aatcgagtat gtgttcgtat tgctgaacac cataccaatg
     2281 tcatttacat tgaaaaaaac gggcaaacta ttttaagcaa accttgtgcc gtcaataacg
     2341 ataatactac tgagatcttt gcttcacttt cggcaaaaga tatatttaat ttttctatgc
     2401 aaatcgaatt ggaaaaaatt cgctttattg agcaagcagc acaattaaat tccgcactct
     2461 cacaagaagg attacgtgcc gattatgggc tacatatcgg aaggacattg caaaaacaga
     2521 ttggtctagg tctaatcagt gatgatttgc ttaatcgcat tgttatagaa accacctctg
     2581 cctctgatgc tagaatgggc ggtgcaagtt tacctgctat gagcaattcg ggttcaggta
     2641 atcaaggaat tacagcaact atgccgattg ttgtcgttgc ccgccattta aatgtcactg
     2701 atgagcaacg cattagagca ctctttttat cacacctcat ggcaatttat attcacagca
     2761 agttaccgaa attatctgca ctctgtgccg taacaaccgc ggctatggga agctgtgccg
     2821 gcatagcgtg gctgctcaca gggaaatttg agaatatcag tatggcaatt agtagtatga
     2881 tcggcgatat tagcggtatt atctgtgatg gtgcttcaaa cagttgtgcg atgaaagtct
     2941 ccaccagcgt aacatcaagc tataaatcca ttctgatgtc tatggataat agccaagtaa
     3001 caggcaacga aggaattgtc gaacacgata ttgatcgttc aattaataac ttatgcagca
     3061 ttgcctctcg tagtatgcaa tacacagacc gccaagtcat cgagattatg gtgaataaac
     3121 caaaacttaa ataatcccgc ctttggtaaa atttcaagca aaaaagaccg cttgcagatc
     3181 acaattactg caagcggtct attttcatta aaattttgca attattgcaa acttattgtt
     3241 attgcaacag cgaaatatcc gccacttgca ggaacaagcc ttgtagcgtg cttaaaatgg
     3301 cgaggcggtt ttgacgcagt ttttggtctt ctgcatttac cattactccg tcaaagaagg
     3361 catcaaccgg ttggcggagg ttcgctaatt tatccagcac agcggtgtag tcgccctgtg
     3421 caatcagcgg ttgaacttcg gctttcaacg caataaccgc ctcggctaat gctttttctg
     3481 ctggctctac gcaagctgaa acatcaatgt cgccaattgc aatatcggct ttggcaagaa
     3541 tgttactcac ccgtttattc gctgcggcta aggcttcggc actgtcaagg gtgcggaagt
     3601 gagacacggc acgcacacgg gcatcgaagt cggcaggctt ggtcggacgg cgggcgagaa
     3661 cggcttggat cacgtccacc gcaatgcctt cgctctcata ccacgcacgg aagcggccga
     3721 gcataaagtc caccacgtcc tcaaccacgt ctttgttggt gagtttatcg ccaaataatt
     3781 gagcagattt cgccactaaa tcggttaaat caagcggtaa atttttctcc acaataatcc
     3841 gcaacgagcc aagtgccgca cggcgtaggg cgaacgggtc ggcactgcct ttcggggctt
     3901 gaccaatgcc gaaaatccct gttagcgtgt caaatttgtc cgccaacgcc accgagcaag
     3961 ccaccaatga atttggcaag ttatcgccag caaaacgtgg catatactgc tcgtttaacg
     4021 ccaccgccac ttcttcatct tcaccgtcgt ggcgggcata gtgcataccc atcacgcctt
     4081 gggtatcagt aaattcaaac accatattgg tcatcaggtc acattttgag aggagacctg
     4141 cacgtttcgc tttggtttca tctgccccga tttgcttcgc aatttcacct gccaacgctt
     4201 caatgcgagc ggttttatca cgcaatgtgc caagctgttg ctggaacaac acggtttcaa
     4261 ggcgtggcag ttggtcttcc aacttctgtt ttaagtcggt tttgaagaag aactccgcat
     4321 cggtcaaacg tgggcgaacc actttttcgt taccctcgat gattttgctt gggtcttccg
     4381 gattgatgtt cgacacaaaa ataaagtgcg gtaacaattt gccgtctttt gaataaatcg
     4441 ggaagtattt ttggtcgcct ttcatggtgt aaactaaggc ttccgccggc acagcaagga
     4501 aacgctcttc aaacttggcg gtgagtacgt tcgggtattc caccaacgag gtcacttcgt
     4561 ccagcaaatc ttcttcaata tcggcaacgc cacccaaagc ggttgctttt tcctgtgatt
     4621 ttgcaagaat taaggcttta cgctcattga aatctgctac cacagatcca cgatctttca
     4681 acagttgcgg atattgatcg gcgttctcaa tttggaactc acgttcacct aagaaacggt
     4741 gaccacgaat agtacggccg cttttcacgc ctaagatctc accttcgatc aactccgccc
     4801 cgaataaaag tgtcacggta tgcacagggc ggacgaactg ctcggtttta tcgccccaac
     4861 gcatcgtttt tggaatcggc aagttcgcca gcgatttggc gataatatcg accaacagat
     4921 ttttagtcgg ctgaccttca atcacggcac gatgcaccaa ccattcgcct ttgtcggtgg
     4981 caatacgctc agcttgctct acggtaatcc cgcagccctt cgcccagcct tccgctgcct
     5041 tagtcggctt gccttcggca tcaaacgctg ccgacaccgc cggccctcgt ttctcaacct
     5101 ctttactcgg ctggctttcc gcaaggctca acaccttcac cgccaaacgg cgaggagcag
     5161 caaaccattc cactttctca aacgaaagac ctgcttggtt taattcgttt tctacattct
     5221 ccgcaaatgc ggtcgctaat tttttgagtg ccttcggtgg cagctcttct gtgccgatct
     5281 cggcaaggaa gttttgtgtt gtcattgttt aaattaccaa tttttatata aatgttcttt
     5341 gatttcttct atttctaatt gtgctatatt ttcattataa agctctctag ctaatttcct
     5401 aattttgatg tctaaatctt ttttataatc ttgagcttct aatagcccat tttgtaattc
     5461 attaaaatgt tcttcagctt cttttctagc attatcattc ttgccattca tt
//
LOCUS       DANJWC010000149         5488 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.149, whole genome shotgun sequence.
ACCESSION   DANJWC010000149 DANJWC010000000
VERSION     DANJWC010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5488
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.149"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..880)
                     /locus_tag="PWX02_002018"
     CDS             complement(<1..880)
                     /locus_tag="PWX02_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDL1729995.1"
                     /translation="MSNNNRSALLGAAFLMATSAIGPGFLTQTATFTQTLLASFGFVI
                     LLSILLDIGAQLNIWRIVAVSEKKAQDIANAVLPGAGYFLALLIVMGGLAFNIGNVGG
                     AGLGLNILTGLSPEMGAVVSGAIAVGVFLFREAGKVMDRFAQIMGFVMIALTIFVAVK
                     ANPPIDDAVVHTFVPEKLDVIAIVTLVGGTVGGYITFAGAHRLLDAGIKGKENLKEVS
                     KSSVSAILIASIMRVVLFLAVLGVVSMGVQLDPKNPAATPFAHVAGDVGLMIFGVVIW
                     AASITSVIGAAYTSVSF"
     gene            complement(1155..1892)
                     /gene="pxpA"
                     /locus_tag="PWX02_002019"
     CDS             complement(1155..1892)
                     /gene="pxpA"
                     /locus_tag="PWX02_002019"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDL1729996.1"
                     /translation="MNHVDLNVDLAEGCGNDHSLLQLVTSANVCCGLHAGDYNEMRKA
                     INWAKENSVRVGAHPSFPDRENFGRTNMQLPDDELKACLLYQLGAAKALCDAENMPMS
                     YVKPHGALYNMAAKDPHLATLIANTVKQFDPNLKLMGLSGSLMLSIAEQHGLETISEV
                     FADRHYLADGSLVPRTRDDAMVESDDEAISQVLQMVLDGTVPTVDGGNVAIKADSICL
                     HGDSEHAVVFAERIKKELQAKGILVKA"
     gene            complement(1879..2808)
                     /locus_tag="PWX02_002020"
     CDS             complement(1879..2808)
                     /locus_tag="PWX02_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HDL1729997.1"
                     /translation="MIYIEKIQGFAHIQDLGRYGYRHLGVSHGGAMDSLALQAGNLLL
                     KNANNAPAIELTLGGMRITFDCDTPFCLTGALVEAELDGTPVFSYYRYTANARQTLTI
                     KRIVLGNYAYLCVAGGFLVPQVLGSASTDLKAQFGGFQGRMLKAGDNIATGRRDSRLS
                     LMSIEPIDFTSHIRATASSEYEAFTADSRERFWQQGWQLQNNSNRMGYRFAEKALELT
                     ASLEMLSYSAPSGTVQVPPDGQPIVLMADAQTTGGYPKIACIIQADLGRLAQNPFGST
                     VQFEQVSREQAVEIYQKDQNYLETIRRKANESR"
     gene            complement(2805..3452)
                     /gene="pxpB"
                     /locus_tag="PWX02_002021"
     CDS             complement(2805..3452)
                     /gene="pxpB"
                     /locus_tag="PWX02_002021"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDL1729998.1"
                     /translation="MLTIHHTSEHSLLCLAEPPAELAKQQQIWRVAEQARALDGVLEV
                     VVGMNNFTLFHAIGTDSTRLIQAVQQLWENDQAQAAQSPRLIEIPVHYGGEFGEDLFE
                     VAQFHNTTPEEIIRRHTEPTYTVFMIGFQPGFPYLNGLPQNLHTPRRNVPRTKVPAGS
                     VGIGGSQTGIYPFTSPGGWQLLGRTELPLFDIQHNPPTLLTAGDNVRFVAESITL"
     gene            complement(3546..4370)
                     /locus_tag="PWX02_002022"
     CDS             complement(3546..4370)
                     /locus_tag="PWX02_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HDL1729999.1"
                     /translation="MKKLLLFLLFIGNPIFAKSPELMLVGQYQDQDISGWVMSEKLDG
                     VRGFWDGQQLISRQGYPLNPPDYFIQDFPPFAIDGELFSERGKFEEISSIVRSAQPKG
                     WHKLKLQVFDVPNAQGNLIERLATLENYLKQHPTLHIAIIEQIPIKDKAHLQQFYQSV
                     LEQGGEGVIVRNPNAGYIQGRSAQILKIKPVLDEECTVVAHHKGKGKYTDKLGAITCE
                     NQRGRFRIGSGFKDKERENPPPIGSIITYKYRGVTESGKPRFATFWRIRNDQISEN"
     gene            complement(4504..5319)
                     /locus_tag="PWX02_002023"
     CDS             complement(4504..5319)
                     /locus_tag="PWX02_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730000.1"
                     /translation="MEHFIGLGVAGNFAGHLEQAGEAADFAKVEAVQPKAIFPFYVPA
                     ENLGDYQFLSTYPLSNTAINFPSDADNLQIEPEVALICEIGYQDQQVVSLTPTHFAAY
                     NDCSIRRPNANKICEKKNWGASSKGLSAIHLPLDNFNKGGTLDNYNIACFHKRNGELN
                     TYGIDSPAVEYSYFHQKLLDWIVERMNNQPDEGPMNNISEMLKQANYPTKAIISIGAT
                     RYTPFGESNLLQVGDVSMVVVYNVKKYSHSQIEEMAKLETFSEDISALIQTVR"
BASE COUNT         1433 a         1302 c         1253 g         1500 t
ORIGIN      
        1 tgaaggaaac cgaggtataa gccgccccaa tcacagaggt aatggaggct gcccaaatca
       61 ccacgccgaa gatcatcaaa ccgacatcgc cggcaacgtg ggcaaatggg gtagccgcag
      121 ggtttttcgg atcaagttgc acgcccattg acaccacgcc cagcaccgct aaaaacagta
      181 ccacacgcat aattgaggcg attaaaatgg ctgacaccga gcttttgctc acctctttta
      241 aattctcttt gcctttaatg cctgcatcaa gcaaacggtg cgcaccagca aaggtgatgt
      301 atccaccaac agttccgccc actagcgtga caatcgcaat cacgtctaat ttttccggca
      361 caaaggtatg caccaccgca tcgtcgattg gcggattggc ttttacggca acgaagatgg
      421 ttaaagcaat catcacaaag cccataattt gtgcaaatct gtccatcact ttaccagctt
      481 cacggaacag gaacacgccc acggcaatcg caccgctgac caccgcaccc atttcagggg
      541 aaagtccggt gagaatgttt agccctaaac ctgcaccgcc cacgttgccg atattaaagg
      601 ctaaaccgcc catcacgata agcagagcca gaaagtaacc tgcccccggc aatacggcat
      661 tggcgatgtc ttgagctttc ttttccgata ctgctacaat tcgccaaata ttcagttgag
      721 caccgatatc aagcaagatc gagagtaaga tcacaaagcc gaagctggca agtagggttt
      781 gggtgaaggt ggctgtttgg gttaagaacc ccggcccgat ggcggaggtc gccattaaaa
      841 atgctgcccc gagtagggcg ctacggttgt tgtttgacat tgtgttggtt ccttttttgt
      901 tgttaatgag tcattattcg tggatttatc atggtaatat tcccctcttt agcaaagagg
      961 ggttagggga gatttggcag tagaactcat cgaagtgaga gagttaataa tgctcaattt
     1021 aacctcttga aattacttat tttctccgta aatcgcttct gtcaaatctc ccccagcccc
     1081 tcttttctaa agaggggagt tcgatctcca agagttttat ttctggtttt ccgtccattt
     1141 tgactaaaac aggatcaggc tttcaccaaa atcccctttg cctgtaactc ttttttaatt
     1201 cgctcggcaa ataccacggc gtgttcgctg tcaccgtgta agcaaatgct gtcggcttta
     1261 atcgccacat taccaccatc aacggttggt acagtgccgt ctaacaccat ttgcaagact
     1321 tggctgatcg cttcatcatc gctttctacc attgcatcat cacgggtgcg aggcacgagc
     1381 gagccgtcag ctaaatagtg gcggtcggca aagacttcgg aaatggtttc caaaccgtgc
     1441 tgctcggcaa tgctcaacat caggctgccg gataagccca tcagtttgag gttgggatcg
     1501 aactgtttga ccgtattggc gataagggtg gcaaggtgag ggtctttggc ggccatattg
     1561 tataaagcgc cgtgcggttt aacgtagctc atcggcatat tttccgcatc acataaggct
     1621 ttggcggctc ccagttggta gagcagacag gctttaagtt catcatcggg cagttgcata
     1681 ttggtacgcc caaagttttc cctatctgga aaactcgggt gagcgccaac tcgcacgctg
     1741 ttttccttcg cccagttaat cgctttacgc atttcattgt aatcaccggc gtgcaagcca
     1801 cagcagacat tcgccgaagt aacgagctgt aataatgaat gatcgttgcc acaaccttcg
     1861 gcaagatcga catttaaatc aacgtgattc attcgctttt ctccgaattg tttccaaata
     1921 attttgatct ttttgataaa tttcgaccgc ttgttcacgg cttacttgct caaattgcac
     1981 cgttgagccg aacggatttt gtgccaaccg cccgagatcg gcctgaataa tgcaggcaat
     2041 tttcggataa ccaccggtgg tttgggcatc tgccatcagc acaatcggct ggccgtccgg
     2101 cggcacttgc accgtaccac tcggggctga gtaggagagc atttcaaggg aagcggtcaa
     2161 ttccaacgct ttttctgcaa aacggtagcc catacggttg ctgttgtttt gcagttgcca
     2221 cccttgttgc caaaagcgtt cacggctatc tgcggtaaag gcttcatatt cgctcgaagc
     2281 ggttgcccga atgtgtgagg tgaaatcaat cggctcaatc gacatcaaag aaagtcggct
     2341 gtctctgcgg cctgtggcga tgttatcgcc ggcttttaac attctgcctt gaaaaccgcc
     2401 aaattgagct ttgagatcgg tacttgccga acctaacacc tgcggcacca gaaaaccgcc
     2461 tgccacgcat aaataggcgt aattgccgag aacaatccgt tttatcgtca gcgtttgacg
     2521 ggcgttggca gtgtagcggt aataggaaaa aaccggcgtg ccgtccagct ccgcctccac
     2581 caatgcaccg gtgaggcaaa atggcgtgtc gcaatcaaag gtaatccgca ttccgccaag
     2641 ggtaagttca atcgcaggtg cattatttgc attttttaac aataaattac ccgcttgtaa
     2701 ggcaaggctg tccatcgcac cgccgtggct cacgcctaaa tggcgataac cataacgccc
     2761 taaatcttga atgtgggcaa aaccctgtat tttttcgata taaatcatag cgttatactc
     2821 tccgccacaa atctaacatt atcgccggct gtaagtagcg ttggcggatt gtgttgaata
     2881 tcaaacaacg gtaactcggt tcgtcctaac agttgccagc cacctggtga ggtaaaggga
     2941 taaatgcctg tttgactacc gccgatgccg accgaaccgg cagggacttt ggtgcgaggc
     3001 acgtttcggc gtggggtgtg taaattttgc ggtaagccgt tgaggtaagg aaagccgggt
     3061 tgaaagccga tcataaacac ggtgtaagtc ggttcggtat gacggcggat aatctcttcg
     3121 ggagtggtat tgtggaattg ggcgacttca aacagatctt cgccaaattc accgccgtaa
     3181 tggacaggga tttcaatcag gcgtggggat tgtgcggctt gggcttggtc gttttcccag
     3241 agttgttgaa ccgcttggat aaggcgtgtg ctatcggtgc caatggcgtg aaatagggta
     3301 aaattgttca ttcctaccac cacttccaac acgccatcta aggctcttgc ctgttctgcc
     3361 acccgccaaa tctgttgctg tttggcaagc tctgcaggcg gttcggctaa gcagagcagg
     3421 gaatgttcgc tggtatgatg aatcgtcagc ataattgccc tcaaattgtt acaaaaaggt
     3481 aacaactttt tacgctgata tatccagact gtcaatttta ttttgttgaa aatgtgaaat
     3541 agctcttaat tttcagaaat ttgatcattt cttattcgcc aaaacgttgc aaatctgggc
     3601 ttgcctgatt ctgtgacacc tcgatattta taggtaatga ttgagccaat cggtggcgga
     3661 ttttctcgtt ctttatcttt aaaaccgctg ccaatccgaa aacgtccacg ttgattttca
     3721 caagtgattg ccccgagttt atcggtatat ttccctttgc ctttatgatg ggcaaccaca
     3781 gtgcattcct catctaaaac cggtttgatt ttcaaaattt gggcagaacg accttgaata
     3841 tagcctgcat tcgggttgcg aacgataacc ccttcgccac cttgttccag cacagattgg
     3901 taaaattgtt gaaggtgggc tttatcttta atcggaattt gctcaataat agcgatatgg
     3961 agcgttggat gttgcttcag ataattctct aaggtcgcca gccgttcaat caaattccct
     4021 tgtgcatttg gcacatcgaa cacctgtaat ttcagcttgt gccaaccttt gggctgagcg
     4081 gaacgcacaa tgcttgaaat ttcctcaaat ttgccccgtt cgctaaacag ttcgccgtca
     4141 atcgcaaaag gagggaaatc ttgaataaaa tagtcgggtg gattgagcgg ataaccttgg
     4201 cggctgatga gctgttgacc gtcccaaaaa cctcgcacgc catcgagttt ttctgacatt
     4261 acccagccac tgatgtcttg atcttgatat tgcccaacga gcattaactc gggcgatttg
     4321 gcaaaaatag ggttaccaat gaagagtagg aataagagca gttttttcat agtctctccg
     4381 aaatagtgcc tccgaatatg caaggcacta taaaggaaag cgtgcaaaca gtcagaaatg
     4441 aatctcaatt tttcgacgca gatcgcaaaa ttttccgaaa aactgatcgc ttgagtgaga
     4501 aatttaacgt actgtttgaa ttaaagcaga aatatcctcg ctaaaggttt ccaacttcgc
     4561 catttcttca atttggctat gagaatattt cttcacattg taaacgacca ccatacttac
     4621 atcacctact tgcaataaat tactttcgcc aaaaggcgtg tagcgtgttg ccccaatgct
     4681 gataatcgct ttagttgggt agtttgcctg ttttaacatt tcggaaatgt tgttcatcgg
     4741 accttcatcg ggctgattat tcattctctc tacaatccaa tcgagcaatt tttgatggaa
     4801 atagctgtac tccaccgcag ggctgtcgat gccgtaggta ttcagctcgc cattacgttt
     4861 atggaaacaa gcgatgttgt aattatccag cgtgccgcct ttgttgaagt tatctaatgg
     4921 aagatgaatt gccgaaagcc ctttagacga agccccccag tttttctttt cacagatttt
     4981 attcgcattc ggacggcgga tagagcagtc attgtaagcc gcaaaatggg ttggcgtgag
     5041 cgaaaccact tgctgatctt ggtagccaat ttcacaaatt aacgccactt caggctcaat
     5101 ttgcagatta tcagcatcac tcgggaaatt aatcgcagta tttgaaagcg gataggtgga
     5161 taaaaactga taatccccca aattttccgc cggcacataa aacgggaaaa tcgcctttgg
     5221 ctgcactgct tccactttcg caaaatccgc cgcctctccg gcttgttcta aatgtccagc
     5281 aaaattgccg gccacgccta aaccgataaa gtgttccata atctactcct taattggtaa
     5341 aagttggata aaattttacc gcttgtaacc aaaaattgcg aatgtttatt ttgatctgat
     5401 tgacagaaaa ttattgaatt gagattaaat ttagccatat tttgaacata aaattagcgc
     5461 tttgttataa aaacaagcaa gcggtcat
//
LOCUS       DANJWC010000150         5486 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.150, whole genome shotgun sequence.
ACCESSION   DANJWC010000150 DANJWC010000000
VERSION     DANJWC010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5486
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.150"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            50..1183
                     /gene="carA"
                     /locus_tag="PWX02_002024"
     CDS             50..1183
                     /gene="carA"
                     /locus_tag="PWX02_002024"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDL1730001.1"
                     /translation="MFKPAILVLTDGSVFSGKSIGADGHTIGEVVFNTAMTGYQEILT
                     DPSYTKQIVTLTYPHIGNTGINLEDKESPNVCASGLIIRDLPLLHSNFRASSSLSDYL
                     IQQNIVAIADIDTRRLTRILREKGAQAGCILVGDATEENIANALAFANSFGSMAGKDL
                     AQSVTCNELYKWTDGEWQLGKGYVPQQQTEFHVVAYDYGVKHNILRMLAERGCRLTVV
                     PAKTSAEAVLALNPDGIFLSNGPGDPEPCDYAITAIRKLLETQKPIFGICLGHQLLGL
                     AAGGKTKKMVFGHHGANHPVQDLDSPKVLITSQNHGFEVDESSLPSNVRVTHRSLFDG
                     TVQGIELTDRPAFSFQGHPEASPGPNDVAYLFDRFVSEMKKVK"
     gene            1286..4492
                     /gene="carB"
                     /locus_tag="PWX02_002025"
     CDS             1286..4492
                     /gene="carB"
                     /locus_tag="PWX02_002025"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDL1730002.1"
                     /translation="MPKRTDINTILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     VLVNSNPATIMTDPNMADVTYIEPIEWKTVEKIIEKERPDAILPTMGGQTALNCALDL
                     SKNGVLKKYNVELIGATEDAIDKAEDRGRFKEAMTKIGLNTPKSFVCHSFEEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNRDEFIAICERGFEASPTHELLIEQSVLGWKEYEM
                     EVVRDRADNCIIVCSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAINPANGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLNE
                     LRNDITGGLIPASFEPTLDYVVTKIPRFAFEKFANADDRLTTQMKSVGEVMAMGRTFQ
                     ESLQKALRGLETGICGFNLLSEEPEKIRRELANPGPNRILYVADAFGAGFSLEEVHHY
                     SKIDPWFLVQIQDLVLEELALEKKQFSDLDANEIRRLKRKGFSDKRIAQLTKVSEKMV
                     RDYRHTYAILPVYKRVDTCAAEFASDTAYLYSTYEEECEAKPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHASLALREAGYETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RVENPWGVIVHYGGQTPLKLANALSDAGVNIIGTSADSIDAAEDRERFQQILNTLGLK
                     QPANRTARNALEAVALANEVGYPLVVRPSYVLGGRAMQIVYNDEELNRYMREAVSVSN
                     DSPILLDHFLNNAIEVDVDCICDGENVLIGGIMQHVEQAGIHSGDSACSLPAYSLSGH
                     VLDEIRKQTNAMAFALGVKGLMNVQFAVQNEVVYVLEVNPRASRTVPFVSKATGRPLA
                     KIAARVMAGETLADLNALEEIIPSKFFVKEAVFPFIKFPGVDTILGPEMRSTGEVMGI
                     GNTFAEAFFKAQLGAGERIPKVGKVFMSVIEEHKPALIYIAKRFQEQGYGLCATFGTA
                     NFLRQHGIAVQTVNKVREGRPHIVDAIKNGEIALVINTVSGEPEVIADSHSIRRTALQ
                     QRVPIYTTLAGADAISYGVKHINEFDVYSVQELEQKAN"
     gene            complement(4567..5358)
                     /locus_tag="PWX02_002026"
                     /pseudo
     CDS             complement(4567..5358)
                     /locus_tag="PWX02_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
BASE COUNT         1566 a         1254 c         1218 g         1448 t
ORIGIN      
        1 cctacttcta aggctacaca gataaaattt gatgattagg agtaaaacaa tgttcaaacc
       61 agcaattctg gttctcactg acggttctgt cttttccggc aaatccattg gtgctgacgg
      121 tcacactatc ggtgaagtgg tatttaacac tgcgatgacc ggttatcaag aaatccttac
      181 cgatccttct tatactaaac aaatagtcac cctgacttac ccccacattg gcaataccgg
      241 cattaattta gaagataaag aatcccccaa tgtttgtgcc tccggcttaa ttattcgtga
      301 tcttcctctt ctccatagca acttccgtgc gtcctcctca ctttcagatt atctcattca
      361 acaaaacatt gtcgctattg ccgatattga tacccgccgt ttaacccgaa ttttacgcga
      421 aaaaggggca caagccggtt gtattttagt aggcgatgcc acagaagaga atattgctaa
      481 cgctttggca tttgccaaca gtttcggttc tatggctggg aaagatttgg ctcaaagtgt
      541 cacctgcaat gaattatata aatggacaga tggtgaatgg caattaggta aaggttatgt
      601 accgcaacaa caaaccgagt tccacgtagt tgcctatgat tacggtgtaa aacacaacat
      661 tttgcgaatg cttgccgaac gaggctgtcg cttaaccgtt gtgccggcaa aaacctctgc
      721 tgaagctgtg cttgcgttaa atccggacgg tattttcctt tctaacggcc cgggagaccc
      781 ggagccgtgc gattatgcca ttaccgcaat ccgaaaatta ttggaaacgc aaaaacccat
      841 tttcggcatt tgcttagggc atcaactttt aggtttagcg gcaggcggta aaaccaaaaa
      901 aatggtgttt ggtcatcacg gagctaatca cccggtacaa gatttagaca gcccaaaagt
      961 gctcatcacc agccaaaacc acggttttga agtagatgaa tccagcttac catctaatgt
     1021 gcgagtgact caccgctcac tgtttgacgg cacggtgcag gggattgaac tcactgatag
     1081 accggcgttt tcgttccaag gtcacccgga agccagcccg gggccgaatg atgttgccta
     1141 tttatttgac cgatttgtca gtgagatgaa aaaagtgaag taaggacgta cctatgtgtg
     1201 tccatcaagc ggtcgaaatt tgcaaaaaac ttaccaaaaa taaccgcttg tagcctgaaa
     1261 taaacgagat aaaagagagc ccaaaatgcc aaaaagaaca gacataaaca ctatcctaat
     1321 tatcggtgcc ggtccgattg tgatcggtca agcgtgtgaa tttgactact ccggagccca
     1381 agcgtgcaaa gcgttgcgtg aagaagggta taaagtggtg ttggtgaact ccaaccctgc
     1441 aaccattatg accgacccga atatggcaga tgtgacttac attgagccga ttgaatggaa
     1501 aacggttgaa aaaatcattg aaaaagaacg ccctgatgcc attttaccga ctatgggcgg
     1561 gcagacagcg ttgaactgtg ccttagatct ttcgaaaaac ggtgtgctga aaaaatataa
     1621 tgtagagctg atcggagcta ccgaagacgc gattgataaa gccgaagatc gtggtcgttt
     1681 taaagaagca atgactaaaa tcggcttgaa tacgccaaaa tcattcgttt gccacagttt
     1741 tgaagaggcc ttagcagcac aagagcaggt tggctttcca accctgattc gcccctcttt
     1801 cacgatggga ggctccggcg gtggtattgc ttataatcga gatgaattta tcgccatctg
     1861 cgaacgtggt tttgaagcct cgcctactca cgaattactg attgaacaat ccgtactagg
     1921 ctggaaagag tatgaaatgg aagttgtgcg tgaccgtgcc gataactgca ttatcgtctg
     1981 ctcgattgaa aacttcgacc cgatgggtgt acacaccggc gactccatta ccgttgctcc
     2041 ggcacaaacg ctaaccgata aagagtatca aattatgcgt aacgcttcgc tggcggtatt
     2101 gcgtgagatc ggcgtggaca ccggcggttc aaatgtgcaa tttgcgatta accctgcaaa
     2161 cggcgagatg atcgtcattg aaatgaaccc tcgtgtgagc cgttcttctg cactcgcatc
     2221 taaagccacc ggcttcccga ttgccaaagt tgcggcgaaa ttggctgtag gctacacact
     2281 caacgaattg cgtaacgaca tcaccggagg gctgattccg gcttcattcg agccgacttt
     2341 agattatgtt gtgaccaaaa ttccacgctt cgcctttgaa aaatttgcca atgcagacga
     2401 ccgattaacc actcaaatga aatcggtagg cgaagtaatg gcaatgggaa gaaccttcca
     2461 agagagttta caaaaagcct tgcgaggctt ggaaaccggc atttgcggct ttaacttgct
     2521 ttccgaagag cctgaaaaaa tccgccgtga acttgccaac cccggaccaa accgtatttt
     2581 gtatgttgcc gatgcctttg gagcaggctt ttcattggaa gaagttcacc actattctaa
     2641 aatcgacccg tggttcttgg tgcaaattca agacttagta cttgaagaat tagctttaga
     2701 gaaaaaacag tttagcgatc tagatgctaa cgaaatccgc cgcttaaaac gcaaaggctt
     2761 ctcagacaaa cgtatcgcac agcttaccaa agtcagcgaa aaaatggtgc gggattaccg
     2821 ccatacttac gctattctac cggtgtataa acgggtagat acctgtgcag ccgagtttgc
     2881 ctcagatacg gcttacctct attctactta cgaggaagag tgtgaagcca aaccgtctga
     2941 tcgcaaaaaa gtgatgattt taggcggcgg accaaaccgt atcgggcagg ggattgaatt
     3001 tgactactgc tgcgttcacg cctcgcttgc tctgcgtgag gcaggttatg aaaccattat
     3061 ggtgaactgc aaccctgaaa ccgtttccac cgatttcgac acctccgacc gcctctattt
     3121 cgagccgcta acattagaag atgtactgga aattgtgcgt gtagaaaacc cgtggggcgt
     3181 gattgtgcat tacggcggtc aaacaccact gaaattagcg aatgccttat ctgatgccgg
     3241 tgtaaacatt atcgggactt ccgccgacag cattgatgcc gccgaagacc gtgaacgttt
     3301 ccaacaaatt ctaaacactc tcggtttaaa acagccggca aatcgcaccg cccgtaatgc
     3361 gttggaagcg gtggcattgg caaatgaagt aggctatccg ctggtggttc gcccatctta
     3421 tgtgctaggt ggtcgggcaa tgcaaattgt gtataacgat gaagagctaa accgctatat
     3481 gcgtgaagcg gtatcggttt ccaacgacag cccgatttta ctcgaccact tcctcaataa
     3541 tgcgattgaa gttgatgtgg attgcatttg tgacggcgaa aatgtactca ttggcggcat
     3601 tatgcaacac gttgagcaag ccggtattca cagtggcgat agtgcctgct cactacctgc
     3661 ctattcgtta agcggtcatg ttttggacga aattcgcaaa caaaccaacg caatggcgtt
     3721 tgctctcggt gtgaaaggct tgatgaacgt acaatttgca gtacaaaatg aggtcgttta
     3781 tgtgttggaa gttaaccctc gagccagccg tactgtaccg tttgtcagca aagctaccgg
     3841 tcgcccactg gcaaaaatcg ctgctcgcgt gatggctggt gaaacccttg ccgacttaaa
     3901 tgcattagag gaaattatcc caagcaaatt cttcgttaaa gaagcggtat tcccattcat
     3961 caaattcccg ggtgtggata ccattctcgg gcctgaaatg cgttctaccg gcgaagtgat
     4021 gggcataggt aatacctttg ctgaagcgtt cttcaaagca caactgggtg ccggtgaacg
     4081 aattccaaaa gtcggcaaag tgtttatgtc ggttattgaa gagcataaac ctgctttaat
     4141 ttacattgcc aaacgtttcc aagaacaagg ctacggttta tgtgctacct tcggcactgc
     4201 aaacttccta cggcaacacg gaattgcagt ccaaacggta aacaaagttc gtgaagggcg
     4261 accgcatatt gtcgatgcga tcaaaaacgg cgaaattgcg ttggtaatca acaccgtcag
     4321 cggagagcct gaagtgattg ccgacagcca ctctatccgc cgtaccgcct tgcaacaacg
     4381 tgtgcctatt tacaccaccc ttgcaggtgc tgatgctatc tcctacggcg taaaacatat
     4441 caatgagttt gatgtgtatt cagtgcagga attagaacaa aaggctaact aaaataaagc
     4501 taaaatgaca agcggtcaga ttttgcaaat tttttacaaa ttctgaccgc ttgtgtttgt
     4561 agaggattat ttagtaaatt tatatgctaa atcattccag cgtaattcat ttttgagttg
     4621 tggtagctcg gtattttggt tgatatggat aaactcaata ccaaaatatt cagcgaacat
     4681 tcgtagcata tcggcatcaa tatctaaact aaatacacta tgatgagcac cgccggctag
     4741 aatccatgct tgagtaccta catcaaaatt tggttgaagc ttccaaaaag catgcccaac
     4801 tggtaaattt ggcattggat ttggtgaggc gatagcgtct aaatccgcaa caaccattct
     4861 aaaacggtct cccatatcta caacggttgc attcactgct tttcccgcct tactactgaa
     4921 aattaagcga acaggatctg ccttaccgcc aatacctagc ggtcgaatgg ctaatgttga
     4981 tttctctttg gcaatagacg aacaaacttc aagcatatga gcccccaata ccacttggtt
     5041 ttgttcatct aaatcatagg tgtagtcttc cataaaggaa gaaccgtttg gtaagccata
     5101 acccatcact ttcacttcac gcacaagagc tgcggttttc caacctcctt caccaccaaa
     5161 tccgtagcct tgttccatta aacgctgaac agccaagccc ggtaattgtt taatcccatt
     5221 tagattttgg aaattatcag taaaagcttt aaatccacca ttatctaaga atgtttttaa
     5281 gccgatttca atacgagcag aatatgcgag agattctctt gctgaaccat tttcttgcaa
     5341 gctatcctca acattataca agcggtcaga ttttgcaaaa aaatgaccgc ttgttgccat
     5401 ttctaaagtg tgatctacct ctcaaattta caaaattttt tataacatat tgatttaaaa
     5461 tgttttttat atttttcctt taattg
//
LOCUS       DANJWC010000151         5424 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.151, whole genome shotgun sequence.
ACCESSION   DANJWC010000151 DANJWC010000000
VERSION     DANJWC010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.151"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(66..659)
                     /locus_tag="PWX02_002027"
     CDS             complement(66..659)
                     /locus_tag="PWX02_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HDL1730003.1"
                     /translation="MWISESKFKQVFPNAVSGIYSIIDKHIEKAGCKTKEQQAMFLAQ
                     CGVECAGYSVFEENLNYSATALLRTFPKYFSQHNVVLYARDKRAIANRVYANRMGNGN
                     EQSGDGWKYRGRGLIQITGKANYIEFAKWEGLPSLVTDPDQITKNLALTVQVAVWYWV
                     SRKLSEFSDILTVTKKINGGTHGLTQRTAYYKKLMAD"
     gene            complement(649..1002)
                     /locus_tag="PWX02_002028"
     CDS             complement(649..1002)
                     /locus_tag="PWX02_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HDL1730004.1"
                     /translation="MNTMPEKNPDIYTQAWNYLVMFLSNHNGWICGVIIAFFTSITKS
                     FLYGKTDTPKRVLAEAILCSVIAGSMRPILIYINADVSLITPIGAGIGLLGTSAIRQM
                     ILKFLNKKAGGENVD"
     gene            complement(1145..1315)
                     /locus_tag="PWX02_002029"
     CDS             complement(1145..1315)
                     /locus_tag="PWX02_002029"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730005.1"
                     /translation="MTEQLNTQSKSGEEDQEKSQDRYAVGWPSRRISDCVQHFRSRKI
                     YPFASNNDVKQG"
     gene            complement(1341..1526)
                     /locus_tag="PWX02_002030"
     CDS             complement(1341..1526)
                     /locus_tag="PWX02_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730006.1"
                     /translation="MYNLDDAIQECREKFGEDKPYFSEAGTLTFAGCKALSRLRDEKL
                     GIKAPPNFPFPRVEDLS"
     gene            complement(1543..1618)
                     /locus_tag="PWX02_002031"
     tRNA            complement(1543..1618)
                     /locus_tag="PWX02_002031"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1583..1585),aa:Trp,seq:cca)
     gene            2035..2229
                     /locus_tag="PWX02_002032"
     CDS             2035..2229
                     /locus_tag="PWX02_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730007.1"
                     /translation="MKLSTNELKALSDERRGVRAKSYKLSLETIALIEQLSKQLDMPQ
                     NQLIKLAVEKLQEQTNLTTN"
     gene            complement(2197..2637)
                     /locus_tag="PWX02_002033"
     CDS             complement(2197..2637)
                     /locus_tag="PWX02_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730008.1"
                     /translation="MLIDIKGVLNLWGSFVRFTDCKGYPTMQAFMRESPQEREKQRYL
                     PRLDDDTLAKVDRQIRKLDPAGKAKAYLANLAKPNDKREELESLESDELQFAILYLRY
                     VAGLENKPIWQKLSLSKGAFENELRLAERFVEGVLIGSEIRLFL"
     gene            complement(2627..2986)
                     /locus_tag="PWX02_002034"
     CDS             complement(2627..2986)
                     /locus_tag="PWX02_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDL1730009.1"
                     /translation="MVELILPYPPSVNDYWRWVSKTRRVVCKRGKDYQWATFLATRNK
                     PKFGGQVEIECDVYFPDNRDRDLDNLGKCILDSLVYSKVIVDDSRKYVKKLTFEDKGN
                     QKGGAVIVRIKERLNVN"
     gene            complement(2979..4007)
                     /locus_tag="PWX02_002035"
     CDS             complement(2979..4007)
                     /locus_tag="PWX02_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDL1730010.1"
                     /translation="MAEGLLLTPYFQTEVGIVFYKVPKDISPAAFGERTLLQPAPTDL
                     ATVKSGKVANFLANSTACKELAEFAKSAKVRSTLNQRDKYSNDLPYMRFVKSIQTCQC
                     RDGKYCHPERVIANIENGAVQLCWHHDKMRMDGEIPTEKLEALADKNWERFIAQQIRL
                     TLRKTQATVIEFADLVLFASIKGLLNELDDNALQHFLGYPALNNSGVNKESNIGFDDV
                     PNARANLKQWAEKLKLKVEPEPLAAFMAKPKLQRFEWRKWLQYVKAQPCMCCGKQADD
                     PHHIIGYGGAMGSKQHDLFVIPLCRIHHDELHRNVGKFEQDYGSQLELLIKFLHRALG
                     LGVLEIDG"
     gene            complement(4134..4727)
                     /locus_tag="PWX02_002036"
     CDS             complement(4134..4727)
                     /locus_tag="PWX02_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HDL1730011.1"
                     /translation="MTDFDRDTWQTPKYLRNWLNHRYAWFHIDGCSNGKNALYSYWIG
                     KAAEGLDEDTLSGQIAEDFLADNLLDVILECVAEWGELLRIFVNPPYSNPLPFVQRAA
                     ELKKAGHIVVMLLPADKTTEWYTIIQQHANEVIDIIGYHDEKGTWRTGRLQFINPVTG
                     KPAQGNNKGSMIVVFDPFIEGLVTRQMPLNKIKEWGK"
     gene            complement(4738..>5424)
                     /locus_tag="PWX02_002037"
     CDS             complement(4738..>5424)
                     /locus_tag="PWX02_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDL1730012.1"
                     /translation="QARKNFRESSEKNPTDNNTNYKNNNDHTTPLNPPTGEPAPAEVV
                     LNYLNSALATLAAQLGERKPVGYSLKPWVKNIAARIRESSVADCCQVVDYLVAKWGRD
                     EKMREYLCPKTIFRQSNFADYLPKSKAWADNGKPVCVNGKWVAPAELEKRLIMPTVEE
                     VRALFQKSLSGNPFKALDFTNKRNLVMYHATINTKNKRPLERDLPMIISQEIKNAVER
                     IDRLKVPTFS"
BASE COUNT         1540 a         1281 c          959 g         1644 t
ORIGIN      
        1 tgccaaatcc agccacacaa acccaaaatc attactgtgg cagctagata aatgtattta
       61 atcatttagt ccgccattag ctttttataa taagctgtac gctgagttag accgtgcgtt
      121 ccgccattaa tctttttagt aacagtaaga atgtcgctaa attcggataa cttgcgactt
      181 acccagtacc aaacagccac ctgtactgtc aatgctagat ttttagtgat ttggtcgggg
      241 tcagtcacta gacttggtaa gccttcccat ttagcaaact cgatataatt tgccttaccg
      301 gtaatttgaa ttaagccacg cccacgatat ttccaaccat cgccactttg ttcattgccg
      361 ttacccattc gatttgcata cacccgatta gcaatagcac gcttatctcg agcataaagt
      421 acgacattgt gttggctaaa gtatttcggg aatgttcgca atagagcagt tgcagaatag
      481 ttaaggtttt cttcaaatac actatatccg gcacattcca caccacactg agccaagaac
      541 atcgcctgtt gctcttttgt tttgcagcct gctttttcaa tgtgtttgtc aataattgag
      601 taaataccac taactgcatt agggaatact tgtttaaact tactttcact aatccacatt
      661 ttcaccacct gcttttttgt tcaagaattt taaaatcatc tggcgaattg cacttgtacc
      721 caataatcca attcccgcac caataggggt aatcagtgat acatcagcat tgatataaat
      781 taaaatagga cgcattgagc cagcaataac gctacacaag atagcctctg ccagcactcg
      841 tttaggtgta tctgttttac catacagaaa tgacttggta atagatgtaa aaaaagcaat
      901 gataacgcca caaatccagc cattatgatt gcttaaaaac attacaagat aattccaagc
      961 ctgcgtatat atatcagggt tcttttcagg cattgtattc atactccacc ccgtttcgag
     1021 gcaataaaaa agcccagtcg tgagactgag ctttggttaa aaattctgct agaatactgt
     1081 tccccaacaa ataaactagc agagggtaaa aatgattgaa tttactctgt cccatcagaa
     1141 gcatttaacc ttgcttcacg tcattattac tggcaaatgg ataaattttc ctacttcgga
     1201 agtgctgaac gcaatctgat atacgtcgag atggccagcc cacggcatag cgatcttgag
     1261 atttttcttg atcttcttca ccgctttttg actgagtatt tagttgttcc gtcatagcaa
     1321 tccctcattg atttttgtag ctaagataga tcttcaaccc ttgggaaagg gaaatttggc
     1381 ggagccttaa ttcctaactt ttcatctctt aatcgagaga gggctttgca accagcaaaa
     1441 gtaagtgttc cagcttcaga aaaataaggc ttatcttcac caaatttttc tctgcattct
     1501 tgaattgcat catcaaggtt atacataaca tttactcacg tttggcaagg gtggctggac
     1561 tcaaaccaac aaccaacgat tttggagacc gttgctctac ctattgagct acacccttaa
     1621 aataaaaagc cccaagcatt tctgctcagg gctgtaaaat tctttttaag ttcaccacct
     1681 atgcaatgaa ccgcaactta ccacaaataa tacactttat acttaagcta tacaacactt
     1741 tttaatcaaa caaatacaaa aaagcccata atttacaaaa ctatgggctt tctaatgaaa
     1801 attcacttgt tttattcggt gttccgaact ataataatct cactttcaac ggttcgggtt
     1861 gaaagtgagt gtgaggctta atcctcacgc ttgaagaagg aacaaacgat gtttaagtac
     1921 attgtcctag ttatcttctt gttagttatc agctccccag cctactaact tgaaataact
     1981 cgctgggggc gaaagctccc agctcttcaa acagggtcat tttaggaatt tagaatgaaa
     2041 ttgtcaacca atgaattaaa agcactatct gatgaaagaa gaggggtgag agcgaaatct
     2101 tacaagctaa gcctcgaaac tattgctctt attgaacaat tatcgaagca gttagatatg
     2161 cctcaaaacc aactgattaa gttagccgtt gagaaactac aagaacagac gaatctcact
     2221 accaattaat actccctcca caaatcgctc ggcaagtcgc aattcatttt caaaggcccc
     2281 cttgctgagc gacaacttct gccaaatagg cttattttct agccctgcaa cataccgcag
     2341 atacaaaata gcaaattgca gttcatcact ctcgagtgat tctaactcct cacgcttatc
     2401 gttcggttta gctaaattgg caaggtatgc tttggcttta ccggctgggt ctagttttct
     2461 aatctgccga tcaaccttgg ctaaggtatc atcatctaat cgaggtaaat agcgttgttt
     2521 ttctctctct tgcggagatt cacgcataaa cgcctgcatt gttggatagc ctttgcaatc
     2581 cgtaaaccta acgaaactac cccataagtt taatacacct ttaatatcaa ttaacattca
     2641 aacgctcctt aattctcaca atgaccgcac cgcctttttg attgcctttg tcctcaaatg
     2701 tgagcttttt cacatatttt cgggagtcat caacaatcac tttgctatat accaacgaat
     2761 ctaaaataca cttacctaag ttatccaaat cccgatctcg gttatcgggg aagtaaacat
     2821 cacactcaat ttcaacctgt ccgccaaatt tcggcttatt tcgagtagca agaaatgttg
     2881 cccattggta atctttacct cgtttacaca ccactcgtct tgttttgctt acccaccgcc
     2941 aatagtcatt tacgcttggc ggatagggca gtattaattc aaccatctat ttctaatacc
     3001 cccaaaccta acgctcgatg taaaaactta atcaacaact ctaattgcga gccgtaatct
     3061 tgctcaaact tacccacatt acgatgtaat tcatcatggt ggattcggca gagtggtatt
     3121 acaaataaat cgtgctgttt actccccatc gctccgccat agccgataat atgatgtgga
     3181 tcgtctgctt gtttaccgca acacatacaa ggctgagctt ttacgtactg caaccacttc
     3241 cgccattcaa accgctgtaa tttcggtttt gccataaatg ccgccaacgg ctccggctcg
     3301 actttcagtt ttaacttttc agcccattgt tttagattgg ctcgagcatt cggcacatca
     3361 tcaaagccga tattactttc tttattcaca ccgctattat tcaaagccgg ataacctaaa
     3421 aagtgctgta aggcattatc atctaactca tttaacaaac ctttgatact cgcaaacaac
     3481 actaagtccg caaattcaat gacggtagct tgtgtcttgc gtaaggttaa gcggatttgt
     3541 tgggcgataa atctttccca gtttttatct gccaatgctt ctaatttttc agttgggatt
     3601 tcaccgtcca ttcgcatttt gtcgtgatgc caacataact gtactgcacc attctcaata
     3661 tttgcgatta ctctttctgg gtgacagtat ttaccatctc ggcattggca cgtctgaatt
     3721 gactttacaa accgcatata cggcaaatca ttactatatt tgtcacgctg atttaacgtt
     3781 gaacgaactt ttgccgattt agcaaactct gccaactctt tacaagcggt cgaatttgcc
     3841 aaaaaatttg caaccttgcc tgatttaacc gtcgctaaat cggtcggtgc aggttgcaac
     3901 aaagtccgct caccgaaagc agcaggagat atatctttag gcactttgta aaacacaata
     3961 cccacttcgg tttgaaagta aggggtaagt agcaaacctt ccgccattat tgcccccatt
     4021 tctcggtttc atcataggct ttcacgaata gcataaatac gccaatacag attaaaaaaa
     4081 tcagtacgcc aatcactgca ccaataccaa taaatagttc ccaataatcc attttattta
     4141 ccccattcct tgattttatt aagtggcatt tgacgagtaa ccaatccctc tatgaacgga
     4201 tcaaacacaa caatcatcga gcctttatta ttgccctgag ccggtttacc tgttactggg
     4261 ttaataaatt gtagtcgtcc tgtacgccaa gtgccttttt catcgtgata gcctatgatg
     4321 tctataacct cattagcgtg ttgctggata atggtgtacc attctgtagt cttatccgct
     4381 ggtaacagca ttaccacaat atgaccggct tttttcagct ctgccgcccg ttgtacaaac
     4441 ggcagtgggt tgctgtatgg cggattcacg aaaatacgca gtaattcgcc ccactctgcc
     4501 acacattcca agatcacatc gagcaagtta tctgccaaga aatcctcggc aatctgacca
     4561 cttagcgtgt cttcgtccaa gccttctgcc gctttgccaa tccagtagct atacaatgcg
     4621 tttttgccgt tagaacaacc gtcaatgtga aaccacgcat agcgatgatt tagccaatta
     4681 cgcaaatact ttggcgtttg ccaagtgtct ctatcaaaat ctgtcattgt ctaatcctta
     4741 cgaaaatgtc ggtactttta gccggtcgat acgctcaacg gcatttttaa tctcttggct
     4801 gataatcatc ggcaaatctc gctcaagtgg tcgcttgttt ttggtgttga tagtggcgtg
     4861 gtacatcact aaattgcgtt tgttggtaaa gtccaacgct ttaaacggat tgccactcaa
     4921 tgatttctgg aacaatgctc gtacttcttc taccgttggc ataatcaagc gtttctcaag
     4981 ctctgctggt gcaacccact taccatttac gcaaacaggc ttgccgttat ccgcccaagc
     5041 ttttgattta ggcagataat ccgcaaaatt cgattggcga aaaatcgttt tagggcagag
     5101 atattcacgc attttttcat ctcgtcccca tttagcgaca aggtagtcca ccacttggca
     5161 acagtccgcc accgaacttt cccgaatgcg tgcagcgatg tttttcaccc aaggcttgag
     5221 tgagtaaccc acaggcttac gctcgccaag ttgcgccgcc agcgttgcca aagccgaatt
     5281 gagataattc aatacaactt cagccggggc aggttccccc gttgggggat taaggggggt
     5341 agtatgatca ttattatttt tataattagt attattatct gtcggatttt tttccgagct
     5401 ttctcggaaa tttttccgag cttg
//
LOCUS       DANJWC010000152         5343 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.152, whole genome shotgun sequence.
ACCESSION   DANJWC010000152 DANJWC010000000
VERSION     DANJWC010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.152"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..466)
                     /locus_tag="PWX02_002038"
     CDS             complement(<1..466)
                     /locus_tag="PWX02_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDL1730013.1"
                     /translation="MKSQIYYDYYPSPIGQLLLLATEEGLIYIELGQEQQTTSLDNFL
                     SAQAASGQILEIFCKTKEILDRYFAGEPLDFQQLDFLAPQGTPFQQSVWKILREIPYG
                     QTTTYGEIATQLGKPSAMRAVGGAVGRNPISILVPCHRVLGKNQALTGFGGGL"
     gene            complement(533..2014)
                     /locus_tag="PWX02_002039"
     CDS             complement(533..2014)
                     /locus_tag="PWX02_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HDL1730014.1"
                     /translation="MKMKNFSKTLIASLVAFSSAGASAAAFQLAEISTSGLGRAYAGN
                     AAVADNASVVATNPALMTLFKQTEISAGGIFVDADVNIDGKLNATGADASHKNVIPTA
                     IVPNAYIVSPINERLSIGGGVNVNYGLISEFDPNYSAGFLGGRTSLNAVNVNFSAAYD
                     LGYGFVLGAGLNAVHAKAELERFLGAGAARFAPAKQFLEDKKVQVQSAIQGLKALEAG
                     IAAGNTALEAQKTQVLAQVNGLLKSQNLNQTITNSAQLERLVNGIQNTNASSTIHKLK
                     GDKWALGWNVGLVYNINERNRLGFTYHSEIDLKFKGKYSNTVPVVIPKMVDTITGGAE
                     MDGTLRLRLPAFWEVSGYHKLTDKLAAQYSYKRTDWSKFQSLEAYDMQGNRLLHKTEN
                     FNDSQRIALGLSYDVNEILTLRTGVAFDESASVTHPSISIPDTDRAWYSVGATVRFTP
                     NLSADVGYSHLRGSNLTFSEDGLATFKSKARANLYGLNVNYKF"
     gene            2361..2813
                     /gene="iscR"
                     /locus_tag="PWX02_002040"
     CDS             2361..2813
                     /gene="iscR"
                     /locus_tag="PWX02_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HDL1730015.1"
                     /translation="MKLTSKGRYAVTAILDIALHGKLEPVSLADISDRQAISLSYLEQ
                     LFAQLRRDGIVQSVRGPGGGYQLGKKPEEISVGMIISAVNENIDVTKCKGSGNCRKDS
                     QCLTHSLWERLEDQIEQFLGTISVADLAAEHADHDCEKEHCHDHPHHH"
     gene            2868..4088
                     /locus_tag="PWX02_002041"
     CDS             2868..4088
                     /locus_tag="PWX02_002041"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HDL1730016.1"
                     /translation="MKLPIYLDYAATTPMDERVAKKMMEYMTKDGIFGNPASRSHKFG
                     WEAEEAVDVARNQIADLIGADSREIVFTSGATESDNLAIKGAAHFYQTKGKHIITVKT
                     EHKAVLDTCRQLEREGFEVTYLDPEEDGILDLAKLKAAIRPDTILVSVMHVNNEMGVI
                     QPIKEIGAICRAKKIIFHVDATQSVGKIEVNVQDLNVDLMSFSSHKLYGPKGIGGLYV
                     CRKPRVRLEAIIHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMARLTVLRDR
                     LYNGFKDIEEVYVNGSMEEGKRIGSNLNISFNFVEGESLMMSLRDIAVSSGSACTSAS
                     LEPSYVLRAIGRDDELAHSSIRFTLGRWTTEEEIDHTIEIVKKAIVKLRELSPLWEMF
                     KEGVDLSKIEWNHH"
     gene            4215..4598
                     /gene="iscU"
                     /locus_tag="PWX02_002042"
     CDS             4215..4598
                     /gene="iscU"
                     /locus_tag="PWX02_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HDL1730017.1"
                     /translation="MAYSEKVIDHYENPRNVGTFDKEASDTGTGMVGAPACGDILRLQ
                     IKVNDQGIIEDARFKAYGCGSAIASSSLITEWVKGKSLEEAGAIKNSDIAEELELPPV
                     KVHCSILAEDAIKAAIADYKEKQAK"
     gene            4707..5030
                     /gene="iscA"
                     /locus_tag="PWX02_002043"
     CDS             4707..5030
                     /gene="iscA"
                     /locus_tag="PWX02_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HDL1730018.1"
                     /translation="MSITLTEAAANRVRTFLENRGKGIGLRLGVKTSGCSGLAYVLEF
                     VDVLNEDDQVFEQHGVKVIVDEKSLTYLNGTQLDFVKEGLNEGFKYTNPNVKNECGCG
                     ESFSV"
     gene            5040..>5343
                     /gene="hscB"
                     /locus_tag="PWX02_002044"
     CDS             5040..>5343
                     /gene="hscB"
                     /locus_tag="PWX02_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HDL1730019.1"
                     /translation="MNNPFTLFDLPVQFQLDNAQLSERYLALQKQLHPDNFAHKSSAE
                     QLEAVQKSADVNEALNILKDPILRAEAMIEIHTGEAKNLEEKSMRDVDFLMQQLEWH"
BASE COUNT         1669 a          986 c         1130 g         1558 t
ORIGIN      
        1 gcaagccacc accgaatccg gttaaggctt gatttttccc taatacccta tggcaaggca
       61 ctaaaatcga aatcgggttt cgcccaactg caccgccgac ggctcgcatc gcacttggct
      121 tgcctagttg ggtggcaatt tcgccataag tagtagtttg cccgtaaggg atttcacgca
      181 aaattttcca aactgattgc tggaatggag tgccttgcgg ggcaagaaaa tccaactgct
      241 gaaaatccaa tggctcgccg gcaaaatagc ggtctaaaat ttctttggtt ttgcaaaaaa
      301 tttctaaaat ctgaccactt gcagcttgag cggaaaggaa attatcaagc gaggtggttt
      361 gttgctcttg ccctagctca atgtaaatca agccttcttc tgtggctaaa agcaataatt
      421 gcccgatagg gcttgggtag taatcgtaat aaatttgaga tttcatgttt ttatgccaat
      481 aaaaaaagag gtgtaattga ttttacacct ctttaaacct taattcatca aattagaatt
      541 tgtagtttac gtttaaaccg taaagatttg cacgagcttt gctcttgaat gtggctaaac
      601 cgtcttcaga gaaggtcagg ttgctaccac gaagatgtga gtaacctaca tcagcagata
      661 ggtttggcgt gaaacgcacg gttgcaccta cagaatacca agcacgatca gtgtccggaa
      721 ttgagattga tggatgagtt acacttgcac tttcatcaaa tgctacacct gtacgcaatg
      781 ttagtatttc attcacatca taagacaagc ctaatgcaat acgttgagag tcattaaagt
      841 tctcggtttt gtgcaatagg cggttacctt gcatatcata agcttctagg ctttggaatt
      901 tgctccagtc tgtgcgttta tagctgtatt gtgcagcaag tttatcggtt agtttgtggt
      961 aacctgacac ttcccagaat gccggtaggc gtaatcttag tgtaccatcc atctctgcac
     1021 cgccggtaat ggtatctacc atcttaggaa taactacagg aacggtatta gaatatttgc
     1081 ctttaaattt aagatcaatt tcagagtggt aagtaaaacc taaacggttt ctttcattga
     1141 tattataaac caagccaaca ttccaaccca atgcccattt atcgccttta agtttatgaa
     1201 ttgttgaact tgcattggta ttttgaatgc cattaaccaa acgttctaat tgagcagaat
     1261 tcgtgatggt ttggtttaaa ttttgagatt ttaataaacc gtttacttga gctaatactt
     1321 gtgttttttg agcttcaaga gcggtattac ctgctgcgat acctgcctca agagctttta
     1381 aaccttgaat tgcactttgt acttgtactt tcttgtcttc caaaaattgt ttagctggag
     1441 caaaacgtgc cgcaccggca cctaagaaac gttctaattc tgcttttgcg tgtactgcat
     1501 tcaaacctgc acctaataca aaaccataac ctaaatcata agccgcacta aaattaacat
     1561 tcacagcatt taatgatgta cgaccaccta agaaaccagc agaatagtta gggtcaaatt
     1621 cagatatcaa accataattt acatttacgc caccaccaat agaaaggcgc tcattgatag
     1681 gtgaaacgat ataagcattt ggaacaatag cggtaggaat gacattttta tgtgaggcat
     1741 ctgcacctgt agcattgagc tttccatcaa tatttacatc agcatctacg aaaataccac
     1801 cggcagaaat ttcggtttgt ttaaataacg tcattaacgc agggttggtt gcaacaacag
     1861 aagcgttatc ggctacagcg gcattacctg cgtaagctct acctaaaccg gatgttgaaa
     1921 tttcagctaa ttggaatgcg gcagcagatg caccggctga gctgaatgct actaatgaag
     1981 cgattaaagt tttgctaaag tttttcattt tcataggaaa tctcttaaaa aatatatatt
     2041 ttagaatgaa taattcataa aagttataag aagtaagaag aaagtgcaga ttttatgtgt
     2101 cgaagtactc tctatcaatt aaatttcaac tgttccgatc agttaggagc aaataagagt
     2161 gaaattgtgc ggtctatgaa ctgtttatct ttggtatttt cggctctttt ttcgtataat
     2221 tagcgttatt gttttaatct gttttaatat ataaaacctt tcacactaat tagtcctaaa
     2281 tgattgacta aattggtggg gtatatcaaa attgagcaac tgttccagaa gattttctgg
     2341 ataatcaatg aggatatttt atgaagctta cctcaaaagg gcgttatgca gtaaccgcca
     2401 ttttagatat tgctttacac ggaaaactag agccggttag cttagccgat atttcagatc
     2461 gccaagcgat ttcactgtct tatttagagc aactgtttgc tcaacttcgc cgtgacggta
     2521 tcgtgcagag cgttcgtgga ccgggtggtg ggtatcaatt aggtaaaaaa ccggaagaga
     2581 ttagcgtggg tatgatcatc tccgccgtga atgaaaatat tgatgtcacc aaatgtaaag
     2641 gtagtggtaa ttgccgtaaa gattctcagt gtttaacgca ttctttgtgg gagcggttag
     2701 aagatcaaat tgagcagttt ttagggacaa tttcagttgc ggatttagcc gcagaacacg
     2761 ctgatcacga ttgtgaaaaa gagcattgtc acgatcatcc tcatcatcac taattgcaaa
     2821 ttttttatca aaaatgaccg cttgggttgt tttaactagg agtaacaatg aaattaccaa
     2881 tttatttgga ttacgcagca accacaccaa tggacgaacg tgttgcgaaa aaaatgatgg
     2941 aatatatgac caaagacggt atctttggta accctgcttc tcgctcacac aaatttggtt
     3001 gggaagcgga agaagcggtt gatgtggctc gtaaccaaat tgccgattta atcggtgcag
     3061 acagccgtga aatcgttttc acttctggtg caacagagtc ggataactta gccatcaaag
     3121 gtgcggctca tttctatcaa accaaaggta aacacattat cactgttaaa accgaacata
     3181 aagcagtatt agatacttgc cgtcagttag agcgtgaagg ttttgaagtg acttacttag
     3241 accctgaaga agacggtatt ttagatttag ctaaattaaa agcggctatt cgtccggaca
     3301 caattttagt ttcggtaatg cacgtaaata acgaaatggg cgtaatccaa ccgattaaag
     3361 agatcggtgc aatctgccgt gcgaagaaaa tcattttcca cgttgatgcg acccagtctg
     3421 ttggtaaaat cgaagtgaat gtacaagact taaatgttga tttaatgtct ttctcaagcc
     3481 acaaattata cggaccaaaa ggtatcggcg gtctatatgt ttgtcgtaaa ccacgtgtgc
     3541 gtttagaagc aattattcac ggtggcggtc acgagcgtgg tatgcgttcg ggtacattgc
     3601 ctgttcacca aattgtaggt atgggtgaag catatcgtat cgctaaagaa gaaatggcaa
     3661 cggaaatggc tcgtttaacc gtgttacgtg atcgtttata taacggcttt aaagatattg
     3721 aagaagtata tgtaaacggt tcaatggaag aaggcaaacg tatcggttca aacctaaaca
     3781 tcagctttaa ctttgttgag ggcgaatcgt taatgatgtc attacgtgat atcgcagtat
     3841 catcaggttc agcttgtact tctgcaagtt tagagccatc ttatgtgtta cgtgcaatcg
     3901 gtcgtgacga tgaattagct cacagctcaa tccgtttcac tttaggtcgt tggacgactg
     3961 aagaagaaat cgatcatact attgaaatcg tgaaaaaagc tatcgtgaaa ttgcgtgagc
     4021 tttcgcctct ttgggaaatg ttcaaagaag gtgtagattt gtctaaaatc gaatggaatc
     4081 accactaatt cagaaaaatt ttgcaagggc aaataatatt tgcccctaca aaataagctt
     4141 tcatcacaaa tagtaaattg aatgtagggg cgaaataggt ttcgccggaa ataggaaata
     4201 aaaggaatac aaaaatggca tatagcgaaa aagtaattga tcattacgaa aacccacgca
     4261 acgtagggac ttttgataaa gaagcatcag acaccggtac aggtatggtg ggagcaccgg
     4321 cttgtggtga tattttacgt ttacaaatta aagtgaacga tcaaggtatc attgaagatg
     4381 cacgctttaa agcatacggt tgtggttctg caatcgcatc aagttctctc attaccgaat
     4441 gggtaaaagg taaatcatta gaggaagcag gtgcgattaa aaacagtgat atcgctgaag
     4501 agttagaatt accaccggta aaagttcact gttcaatctt agcagaagat gcaatcaaag
     4561 ctgcaattgc agactacaaa gagaaacaag ctaagtaata tgatacaatc aatgcaagcg
     4621 gtcgattttt gcaaaaaatt tgcaaaattt aaccgcttgc atttgctatt tctatcttga
     4681 tttaatccat tcacataagg aaaactatga gcattacctt aacagaagct gccgcaaacc
     4741 gagttcgtac attcttagaa aacagaggta aaggtatcgg gctaaggctt ggtgtgaaaa
     4801 cttccggttg ttcggggctt gcctatgtgc ttgaatttgt tgatgtatta aatgaagacg
     4861 atcaggtttt cgaacaacac ggcgtaaaag ttattgtcga tgaaaaaagc ctgacttact
     4921 taaacggtac gcagctggat tttgtgaaag aaggcttaaa cgaaggcttt aaatacacca
     4981 acccgaatgt taaaaacgaa tgcggctgtg gcgaaagttt tagtgtgtaa ggaactgtaa
     5041 tgaataaccc ttttacttta tttgatttac ccgttcaatt tcaacttgat aacgcccagc
     5101 tttctgagcg ttatcttgct ttacagaaac agcttcaccc agataacttt gcccacaaat
     5161 caagtgctga acagttagaa gcggtgcaaa aatctgctga tgtcaatgag gcactcaata
     5221 ttcttaaaga tccgatttta cgggcagaag cgatgattga aatccataca ggtgaagcca
     5281 aaaatctcga agaaaaaagt atgcgagatg tggatttctt aatgcagcaa ctcgaatggc
     5341 acg
//
LOCUS       DANJWC010000153         5321 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.153, whole genome shotgun sequence.
ACCESSION   DANJWC010000153 DANJWC010000000
VERSION     DANJWC010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.153"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..147
                     /locus_tag="PWX02_002045"
     CDS             <1..147
                     /locus_tag="PWX02_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDL1730020.1"
                     /translation="YNGYVVDFLHFYWDIYHYPVFNIADIAICVGAGLLILEAFITKK
                     EPKK"
     gene            158..1102
                     /gene="ispH"
                     /locus_tag="PWX02_002046"
                     /gene_synonym="lytB"
     CDS             158..1102
                     /gene="ispH"
                     /locus_tag="PWX02_002046"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDL1730021.1"
                     /translation="MKILLANPRGFCAGVDRAISIVELALEIHGAPIYVRHEVVHNKF
                     VVDGLKAKGAIFVEELHEVPDDAIVIFSAHGVSQVVRQEAKNRGLKVFDATCPLVTKV
                     HMQVARASKKGTKAILIGHEGHPEVIGTMGQYNNQEGGIYLVESVEDIANLPVSNNDE
                     LTFMTQTTLSIDDTIGVIEALKEKYPAIQGPRKNDICYATTNRQHAVRELAEQSQLVL
                     VVGSKNSSNSNRLAELASRMGVMSKLIDGPQDIDPTWLEGIETIGITAGASAPEVLVQ
                     SVISHLKTLGVTEVETLVGNEEDVVFEVPKELRIKEVK"
     gene            1102..1350
                     /locus_tag="PWX02_002047"
     CDS             1102..1350
                     /locus_tag="PWX02_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HDL1730022.1"
                     /translation="MTEINRFKLEWACRRGMRELDKMIMPFYQNHFNELNETQQQAFV
                     EMLNYSDPELFRWVMLQAPAPTEEIAELIRLMRDKIET"
     gene            1530..1862
                     /locus_tag="PWX02_002048"
     CDS             1530..1862
                     /locus_tag="PWX02_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF413 domain-containing protein"
                     /protein_id="HDL1730023.1"
                     /translation="MADSFSVTRRFFDDKNYPRGFSRHGDYTIRESQTLEQFGQACLA
                     LESGERKPATQEEKRFVAVMKGEEVAETDIEKAWLKYRTLTSKTKRIYTLSGNAGSDA
                     GDDFSAAE"
     gene            1927..3018
                     /gene="trmA"
                     /locus_tag="PWX02_002049"
     CDS             1927..3018
                     /gene="trmA"
                     /locus_tag="PWX02_002049"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HDL1730024.1"
                     /translation="MTLPIEQYSDLLAKKTENLTALLKPFNPPAIEVFASKPSHFRMR
                     AEFRVWHEEGELYHIMFNPATKARYRVDSFPIASELINHMMTALLAEIKGNELLTRKL
                     FQIDYLSTLSGEIAVSMLYHKSLNEEWVEQANALKSRLEQQGFKVQIIGRANKQKIAL
                     DCDYVEEILPVHGKNYVYRQVENSFTQPNAEMNIKMLEWARNCTKNSEGDLLELYCGN
                     GNFSIALAENFRKVLATEISKSSVQSAQYNIEKNGIVNLQIIRMSAEEFTQAMNGVRA
                     FYRLKGIDLNAYECNTIFVDPPRAGLAQATLNMVQAYERILYISCNPETLAENLQQLS
                     QTHRIEKAALFDQFPYTHHIESGVWLIRK"
     gene            3110..3199
                     /locus_tag="PWX02_002050"
     tRNA            3110..3199
                     /locus_tag="PWX02_002050"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3144..3146,aa:Ser,seq:tga)
     gene            3355..3726
                     /locus_tag="PWX02_002051"
     CDS             3355..3726
                     /locus_tag="PWX02_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HDL1730025.1"
                     /translation="MKYLYIICGFICIGLGIIGAVLPGLPTTPFLLLALFCFTKGSER
                     IRQWFIQTKLYKKYLQEYDEKRAMTMKQKITILAISFPFTLFAFFALPHIGGKIALVV
                     LVIIQYWYFFCKMKTLEPENA"
     gene            complement(3723..4634)
                     /gene="xerC"
                     /locus_tag="PWX02_002052"
     CDS             complement(3723..4634)
                     /gene="xerC"
                     /locus_tag="PWX02_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDL1730026.1"
                     /translation="MTTSNPLFQQTRPYWDFLRIEKQVSPHTLSNYQRQLMAVSELFS
                     QAGIENWEEVESSSVRWMLSQSHKQGLSAKSIGLRLVALRQWFVYLIKIGRMSANPTL
                     GVKSPKVGKHLPKNIDAEQVGQLLAIESDEPSDIRDIAMMELMYSSGLRLSELQGLDL
                     GDMDLSAREVRVQGKGSKERIVPIGSQALKALNRWLEMRLQFKPQDNAVFLNKRGGRL
                     SHRSIQLAMKKWGERQGLESHLHPHKLRHSFATHMLEASGDLRAVQELLGHSSLSTTQ
                     IYTHLDFQHLAKIYDSAHPRARRKQED"
     gene            complement(4701..4991)
                     /locus_tag="PWX02_002053"
     CDS             complement(4701..4991)
                     /locus_tag="PWX02_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5377 domain-containing protein"
                     /protein_id="HDL1730027.1"
                     /translation="MSVKTEVLFSNHWNVRVSDPGFEGSPSHYFETVYLTLEAFINGE
                     NVHYEFTRKVEQEVKTHRSFTDVNELFEFLGEYLSPVALGNVGVRIGQLGLA"
BASE COUNT         1595 a         1128 c         1079 g         1519 t
ORIGIN      
        1 tacaacggct atgtagttga tttccttcat ttttattggg atatttatca ctaccctgtg
       61 tttaatattg ccgatattgc gatttgtgtg ggtgcaggct tactgatttt agaagccttt
      121 ataaccaaaa aagaaccgaa aaaataagag tgattaaatg aaaattttat tagcaaaccc
      181 acgcggtttt tgtgccggcg tggatcgagc catttcgatt gttgaattag ccttagaaat
      241 tcacggtgca cctatctatg ttcgccacga agtggtacat aacaaatttg tggtggatgg
      301 cttgaaagcc aaaggggcta tttttgttga ggagctacac gaagtgccgg acgatgctat
      361 cgtgatcttc tcagcccacg gggtgtcgca agtagttcgc caagaagcca aaaatcgtgg
      421 cttaaaagtg tttgatgcca cttgcccact cgtgaccaaa gtgcatatgc aggttgcccg
      481 agccagtaaa aaagggacaa aagcaatttt aatcggtcac gaaggtcacc ctgaagttat
      541 cggtacaatg ggacaataca ataaccaaga aggtggcatt tatttggtag aatcggtgga
      601 agatattgcc aacttgccgg tttccaacaa cgatgaatta acttttatga cgcaaaccac
      661 actttccatt gacgatacca ttggcgtgat tgaggcgtta aaagagaaat atccggcaat
      721 tcaaggccct cgcaaaaatg atatttgtta tgctaccacc aatcgccaac acgcagtgcg
      781 ggaactggca gaacaatcac aattagtgtt agtggtaggt tcaaaaaact cctcgaactc
      841 aaatcgtttg gcggaactcg cctctcgaat gggtgtgatg tcaaaactga ttgatggtcc
      901 gcaagatatt gatccaactt ggttggaggg tattgaaact atcggtatta ccgccggtgc
      961 ttccgctcct gaagtgttgg tgcaatcagt aatttcgcat ttaaaaaccc ttggcgtaac
     1021 cgaagttgaa accttagtcg gcaacgaaga agacgtggtg tttgaagtcc cgaaagagtt
     1081 acgcatcaaa gaggtaaaat aatgacagaa atcaaccgct ttaaattaga atgggcgtgc
     1141 cgacgtggta tgcgtgagtt agacaaaatg attatgccgt tttaccaaaa tcattttaat
     1201 gaactcaatg aaacccaaca gcaagctttc gttgaaatgc tcaattacag cgacccggaa
     1261 ctgtttcgct gggtaatgct gcaagcacct gccccaaccg aagaaatcgc tgaattgatt
     1321 aggttaatgc gagataaaat cgaaacatag ccccaccgct tgcaacttgc aagcggtgat
     1381 tttttgcata aactttgcat attgggcgtt aatgcaaaaa attcgctaaa tttgaccgct
     1441 tttttacatt aaggcattga tttattcgct gtttttgttt aatgtatgcc gattttattt
     1501 tccctaattt caaacaacga ggcattatta tggcagacag tttcagcgtt acgcgccgct
     1561 tttttgacga taaaaattat ccacgtggtt tctctcgcca cggcgattac accattcgtg
     1621 aatcacaaac tttagaacaa ttcggtcaag cttgcttggc tttagaaagt ggtgaacgta
     1681 agccggcaac tcaagaagaa aaacgtttcg ttgcggtaat gaaaggcgaa gaggtagcag
     1741 aaactgacat tgaaaaagcg tggttaaaat accgcacatt aactagtaaa acaaaacgca
     1801 tctacaccct ttcaggcaat gcaggtagcg atgccggcga tgacttcagt gctgctgaat
     1861 aatttctaat ttaaaaccac gaagtaattc gtggtttgtt ttttcatttg ctatttgtgg
     1921 taaaaaatga ctcttcctat cgaacaatat tccgatcttc ttgcaaaaaa aaccgaaaat
     1981 ttgaccgctt tactcaagcc gtttaacccg cctgcgattg aggtatttgc ctcaaagcca
     2041 agccatttcc gtatgcgggc agaatttcga gtgtggcacg aggaaggcga gctgtaccac
     2101 attatgttca accctgcaac caaagctcgt tatcgggtgg atagcttccc tattgccagt
     2161 gagctgatta accatatgat gaccgcatta ttggcggaaa ttaaaggcaa tgaactactg
     2221 actcgcaagc tgttccagat cgattattta agcaccttaa gcggtgaaat tgcagtctca
     2281 atgctttacc acaaaagcct gaatgaagaa tgggttgaac aggcaaacgc cttaaaaagc
     2341 cgtttagagc aacaaggttt caaagtgcaa attatcgggc gtgccaataa acaaaaaatc
     2401 gcactggatt gcgattatgt ggaagaaatt ctaccggtac acggcaaaaa ttatgtgtat
     2461 cgccaagtgg aaaacagctt tacccagccg aatgcggaaa tgaatattaa aatgctggaa
     2521 tgggcgagaa attgcacaaa aaatagtgaa ggcgatttgc ttgagcttta ctgcggcaac
     2581 ggcaattttt cgattgcctt ggcggaaaac ttccgcaaag tgctggcaac cgagatctca
     2641 aaatcttcag tgcaatcggc tcaatacaat attgaaaaaa atggcattgt taacctacaa
     2701 attatccgaa tgtcggcaga agaatttacc caagcgatga acggtgtgcg agccttttat
     2761 cgcttaaaag ggattgattt aaacgcctac gaatgcaata ccatttttgt tgatccacct
     2821 cgtgcagggt tagctcaagc tacattaaat atggtgcaag cgtatgagcg gattctctat
     2881 atctcttgca atccggaaac ccttgccgag aatttacaac aattaagcca aacccatcgg
     2941 attgaaaaag ccgctctttt tgaccaattc ccttacaccc accatattga aagtggcgtt
     3001 tggttaatta gaaaataaat tgtgctgaaa aaaagaacga ataagcagct tttaagattt
     3061 attacttgac gatgttcgca ataatctcta ttattgcaac attttcaacg gaggaatggc
     3121 agagcggttg aatgcaccgg tcttgaaaac cggcgagggt taacgccctc cgtgagttcg
     3181 aatctcactt cctccgccag tctttaaacc tgttgagcaa tcagcaggtt tttttatttc
     3241 atttgcaaga aaatcagata aaaccaccgc ttgaatccat cgctataaac tggttgaaat
     3301 cagcctaaag ttgctttagg ataattgcga ttcattatca ataacttaat tattatgaaa
     3361 tacctttata ttatctgtgg ttttatttgt attggtttag gcatcatcgg ggcagtatta
     3421 ccgggcttgc cgaccacacc gttcttactt ttggctctgt tctgctttac taaaggctcg
     3481 gagagaatcc gccagtggtt tattcaaacc aaattataca aaaaatatct gcaagaatat
     3541 gatgagaaac gggcaatgac gatgaagcaa aaaatcacga ttttagcgat ttcgtttccg
     3601 tttacactat ttgctttctt tgcactgccc catatcggtg ggaaaattgc gttagtagtg
     3661 ttagtgataa ttcaatattg gtatttcttc tgcaaaatga aaacccttga accggagaat
     3721 gcctaatctt cctgcttccg ccttgctctt gggtgggcag agtcataaat tttagctaag
     3781 tgttggaaat ccagatgcgt ataaatttga gtggtggaca ggctactatg acccaataat
     3841 tcctgaaccg ctcgcaaatc accgcttgcc tctaacatat gagtcgcaaa agagtgacgt
     3901 aatttatgtg gatgcagatg gctttccagc ccttgtcgct caccccattt cttcattgcc
     3961 aactgaatgg aacgatgaga taaccgccca cctcgcttat tcagaaacac cgcattatct
     4021 tgcggcttaa attgcaaacg catttctaac cagcgattta acgcttttaa cgcttgcgag
     4081 ccaatcggca caattcgctc tttgctaccc ttaccttgca cccgtacttc tcgtgccgat
     4141 aaatccatat cgcctaaatc cagcccttgt aattccgata aacgcaagcc ggaactatac
     4201 atcagctcca tcatcgcaat atctcgaata tcgctcggct catcactctc aatcgccagc
     4261 aactgcccga cctgctccgc atcaatattt ttcggcaaat gcttacctac tttcggcgat
     4321 ttcaccccta aagtcgggtt tgccgacatt cgcccgattt taatcaaata gacaaaccat
     4381 tgccgtaacg ccaccaagcg taagccgata cttttcgcac tcaacccttg cttatggctt
     4441 tggcttaaca tccaacgcac cgagctactt tcgacctctt cccaattttc aatgcctgct
     4501 tgcgaaaaaa gctcgctcac cgccattaat tggcgttgat aattgcttaa ggtatgcggg
     4561 ctaacttgct tttcaatgcg taagaaatcc caataggggc gggtttgttg gaaaagaggg
     4621 ttggaagtgg tcataaacgt aataacaagc ggttaatttt taggaaaatg ttgcaagggc
     4681 aaaagatttg ccctttgaga ttaggctagc cccaactgac cgatacgcac gccaacatta
     4741 ccaagtgcaa cagggcttaa atattcgccg agaaattcaa acaattcatt cacatcagta
     4801 aaactgcgat gagttttcac ttcttgctcc actttacgag taaattcata atgtacattt
     4861 tcgccgttaa taaaggcttc cagcgttaaa tatacggttt caaaatagtg gcttggcgag
     4921 ccttcaaagc ccggatcact cactcgaaca ttccaatgat tactaaataa aacttcggtt
     4981 tttactgaca ttgtgttttc cttcttccta taagtacgta cattatttta atcaagatag
     5041 attgaatttg ccacttttcc tagtcagaat cgaggcaaat cttatttggt ttgtcctctt
     5101 atatctttcc ctcaaaatat gatcttggtc aaaaaatact gacttttaac tcttaataaa
     5161 gttaaatata attgacttta aaatttactc attcttataa ttcacaaaaa ttggagtaaa
     5221 agttatgcca gataaattag agtatcttca tgatttagac aaagaaatta tgctgtctta
     5281 tttctcatta agtgaagcaa tagcagggtt attaggagaa t
//
LOCUS       DANJWC010000154         5283 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.154, whole genome shotgun sequence.
ACCESSION   DANJWC010000154 DANJWC010000000
VERSION     DANJWC010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5283
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.154"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(230..1870)
                     /gene="pgi"
                     /locus_tag="PWX02_002054"
     CDS             complement(230..1870)
                     /gene="pgi"
                     /locus_tag="PWX02_002054"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013527751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDL1730028.1"
                     /translation="MQKINPVQTFAWNALEQHKADNLSIPQLFAEDPNRFDKYSLRFE
                     DQLLVDFSKNAINGKTLTLLRQLANECGLESAKNAMFSGKKINRTENRAVLHVALRNR
                     ANTPIAVDGKDVMPEVNAVLAKMKDFCERVISGEWKGYTGKTITDVINIGIGGSDLGP
                     YMVTEALRPYKNHLNMHFVSNVDGTHIAEVLRKVNPETTLVLVASKTFTTQETMTNAL
                     SARKWLLDSAKDESAVAKHFVALSTNAKEVEKFGIDTANMFEFWDWVGGRYSLWSAIG
                     LSIALSLGFENFEQLLAGAHAMDKHFLITPIEKNIPTTLALVGIWNNNFLGAESEAIL
                     PYDQYMHRFAAYFQQGNMESNGKFVGRDGKPVTHQTGPIIWGEPGTNGQHAFYQLIHQ
                     GTKLIPCDFIAPAQTHNPLSDHHNKLLSNFFAQTEALAFGKSKETVEKEFLDAGKTLE
                     EVAEIVPFKVFSGNKPTNSILVQKITPYSLGALIAMYEHKIFVQGVLFNIYSFDQWGV
                     ELGKQLANRILPELETDEKINSHDSSTNGLINQFKAWR"
     gene            complement(1903..2151)
                     /locus_tag="PWX02_002055"
     CDS             complement(1903..2151)
                     /locus_tag="PWX02_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HDL1730029.1"
                     /translation="MLTPKNLEAIAQQLHNALPQSLKNVGNDLDEKFKQILQAQLARL
                     DVVTREEFDVQSQVLLRTREKLTELEKRLDELTQRTDE"
     regulatory      2276..2320
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            2324..2992
                     /gene="queC"
                     /locus_tag="PWX02_002056"
     CDS             2324..2992
                     /gene="queC"
                     /locus_tag="PWX02_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDL1730030.1"
                     /translation="MTHQKPKAVVIFSGGQDSTTCLFQAIAEFGKENVEVVTFQYGQR
                     HSIELEKARWISNDLGVKQTLIDTSVIKAITTNALMDNNAEIQQQGSTPNTFVDGRNA
                     LFLLYTAIYAKGQGIQTIFTGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYNFNIRTP
                     LMYLTKKETWALADKLGAFDYIRDHTHTCYLGVEGGCHDCPSCVLREKGLNEYLAERE
                     VSNV"
     gene            2985..3407
                     /gene="queD"
                     /locus_tag="PWX02_002057"
     CDS             2985..3407
                     /gene="queD"
                     /locus_tag="PWX02_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDL1730031.1"
                     /translation="MFKIAKEFSFDMAHMLDGHDGKCQNLHGHTYKLQVEVAGELFQE
                     GAKRGMVMDYSDLKAVVKQHILEPMDHAFIYDLQSERESQVAQLLIDLNSKVYGIPTR
                     TTAEEMAKYMFEKLEKVGLPVSLIRLWETPTSYCEYSK"
     gene            3407..4033
                     /locus_tag="PWX02_002058"
     CDS             3407..4033
                     /locus_tag="PWX02_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDL1730032.1"
                     /translation="MAELISNPRFPIVEIFESLQGEGFNTGMPAIFVRFGKCNLTCPW
                     CDTNYNQFEQWTLSDILAKVRSYSAKNVIITGGEPTIQPNLSLLLEQFKKEGYFLAIE
                     TNGLKEVPAQIDYIATSPKRMYQEKYQRRCIEFANEVRIVVDGEVQGFCEQIESQIKA
                     EHYYLSPCEVEGKMNLLETITQIGLLNQRPNKPKWQLSIQTHKIVGIE"
     gene            complement(4129..5178)
                     /locus_tag="PWX02_002059"
     CDS             complement(4129..5178)
                     /locus_tag="PWX02_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730033.1"
                     /translation="MKFNKKLILTFAATLVLSACGGSSSGGSPSTPNQPKPVLVPKTQ
                     NTPQVQNAPQVQNVPQVQNVPQMENNTQSQNNSQIQDQPEVAPPATQIAPSIPEKKID
                     GSFDKIGTVILDAEKQTITLNKFILVDKPDSPKKYDRVSGKPIIKEKNQIILPLADIS
                     TGALTSDQNADYLIGRSGDLFYGVYHDTQSNILVEEADKFSKYFVIYDENRVNSKVSQ
                     DLTATYDKKGGVVYGANAGIKEFEARLNKLGDVNIEFKNGKANGNISDENSVKPIFTI
                     TGDTKSLVISPTADNPVISVILNERKQSYSQGMEKAVMDIKFIDSAKGAGDQKYLIGE
                     AKSESWQAIMVSEKQ"
BASE COUNT         1557 a          959 c         1070 g         1697 t
ORIGIN      
        1 tgagtgaaga gtagaggtaa aatagtaagc ctttttaacc agtttattta ttgtacctat
       61 tttagaaaaa agaaaagtag aaaagagata ttaggtaaac tcatagtgca aaagaatata
      121 gaaaaaactg cttgtgtaga aatggtgctt agttcagtcc tcagctaaaa ttttagaaaa
      181 aaagcataga gattttttac ctctatgctt tttattgtat tgactatatt taacgccaag
      241 ccttgaattg gttaattaaa ccatttgtgg agctgtcgtg gctgttgatt ttttcatcgg
      301 tttcgagttc gggtaaaatg cggtttgcaa gctgtttgcc taactccacg ccccattggt
      361 cgaaactgta aatgttgaac agcacgcctt gcacgaagat tttgtgttca tacattgcaa
      421 ttaatgcccc taagctgtac ggggtgattt tttgcaccaa gatagagttg gtcggtttgt
      481 ttccgctgaa cactttaaac ggcacgattt cagctacttc ttccaacgtt ttacctgcat
      541 ctaaaaactc tttttcaact gtttcttttg atttaccgaa agctagtgct tcagtttgag
      601 caaagaagtt agaaagcagt ttgttgtgat gatcgcttaa cggattgtga gtttgtgccg
      661 gtgcgataaa atcacaagga attaattttg tgccttgatg aatgagttga tagaatgcgt
      721 gctgaccgtt agttcccggt tcgccccaaa taatcggacc tgtttggtga gtaaccggtt
      781 tgccgtcacg ccccacaaat ttaccgtttg attccatatt gccttgttgg aaataagccg
      841 caaagcggtg catatattgg tcgtaaggta aaatcgcttc tgattcagca cctaagaaat
      901 tgttattcca aatgccgact aaagctaacg tagttggaat gtttttctca atcggcgtga
      961 ttaagaaatg cttatccatt gcgtgagcac cagcaagtag ctgctcgaag ttctcaaaac
     1021 ctaatgaaag ggcaatcgaa agcccgatgg cagaccataa tgagtaacga ccgccaaccc
     1081 aatcccagaa ttcaaacata ttagcggtat caataccgaa tttttccact tctttggcat
     1141 tggttgaaag tgccacgaaa tgttttgcta ccgcagattc atctttggca gaatcaagta
     1201 gccatttgcg agctgaaagg gcattggtca tcgtttcttg agtggtaaag gtttttgaag
     1261 ccactaacac taacgtggtt tcaggattaa ctttacgcaa cacttcagca atatgcgtgc
     1321 cgtccacgtt agacacaaag tgcatattaa ggtgattttt ataagggcgt aatgcctctg
     1381 ttaccatata aggacctaga tccgagccac caataccgat attgatcaca tcagtaatcg
     1441 ttttgccggt atagcctttc cattcgccgg aaataacacg ctcgcagaaa tctttcattt
     1501 tcgctaacac ggcattcact tccggcatta catctttgcc atcgactgcg attggtgtat
     1561 tggcacggtt acgcagtgca acgtgcaata cggcacggtt ttcagtgcgg ttgatttttt
     1621 tgccggagaa catcgcattt tttgctgatt ccaaaccaca ttcatttgcc agttggcgaa
     1681 gtaaggttaa ggtttttccg ttgatcgcat tttttgaaaa atcgactaat aattgatctt
     1741 caaaacggag ggaatatttg tcgaaacggt ttggatcttc ggcaaaaagt tgaggaatag
     1801 aaaggttgtc tgctttatgt tgctctaagg cattccaagc aaaagtttgc acagggttaa
     1861 ttttttgcat ctgagtttcc ttatactaaa ttaaaaaatc gattattcat ctgttctttg
     1921 agtcagttca tcaagacgtt tttctaattc ggttaatttt tcacgagtgc ggagtaacac
     1981 ttgagattga acatcaaatt cttcacgagt gacaacatca agacgggcta attgggcttg
     2041 aaggatttgc ttaaattttt catctagatc attaccgaca ttttttaagc tttgcggtaa
     2101 ggcgttatgg agttgttgag caatcgcttc taagttcttc ggcgtgagca tatttaaatt
     2161 ccttactata aatgagtatc tcaattttat tgaattttgg gtttaagaga aagcaaattc
     2221 tttgtaaaat atcacctttc gcaaaataca agcaaaaaaa gaccgcttgt attttttatt
     2281 tgtggttcgt aaacctccca cacaaaaaaa ctaaggacac aaaatgactc atcaaaaacc
     2341 aaaagccgtt gtgatttttt ccggcggtca agactctacc acttgtttat tccaagctat
     2401 tgctgaattt ggcaaagaaa atgtggaagt cgttaccttt caatatgggc aacgccattc
     2461 gattgaactt gaaaaagcac gctggatttc taacgattta ggcgtcaaac aaacgctgat
     2521 tgatacttcg gtgattaaag cgattactac caatgcgtta atggataata atgctgaaat
     2581 tcaacaacaa ggcagtacac ccaatacctt tgtggatggg cgtaatgcct tatttctgct
     2641 ttataccgcc atttatgcca aagggcaggg cattcaaact atttttaccg gcgtgtgtga
     2701 gaccgatttt agtggttacc ctgattgccg agatgtattt gtaaaatcaa tgaatgtaac
     2761 gctaaattta gcgatggact ataatttcaa cattcgcacg ccattgatgt atttaactaa
     2821 aaaagaaact tgggcattag cagataagct gggtgctttt gattatattc gagaccatac
     2881 ccacacctgt tatttaggcg ttgaaggtgg ttgccacgac tgcccgagtt gcgtattgcg
     2941 agaaaaaggc ttgaatgaat atttagcaga aagggaggta agcaatgttt aaaattgcta
     3001 aagaatttag ttttgatatg gctcatatgc tagatggaca cgatggcaaa tgccaaaact
     3061 tacacggaca cacctacaaa ttgcaagttg aggtggcagg tgagctattt caagagggag
     3121 ctaaaagagg aatggtaatg gactattcgg atctaaaagc agtggtaaaa caacatattt
     3181 tagagccgat ggatcacgct tttatttacg acttacagag tgagcgtgaa agccaagtag
     3241 cacaattatt aattgattta aactccaagg tttatggtat tccaactcgt accacagccg
     3301 aagaaatggc gaaatatatg tttgaaaaat tagagaaagt gggcttaccg gttagcctaa
     3361 ttcgcttgtg ggaaacgcct acttcttatt gtgagtacag caaataatgg cggagttaat
     3421 ttcaaaccct cgttttccga ttgttgagat ctttgaaagc ttacaagggg aaggctttaa
     3481 taccggaatg ccggctattt ttgtacgttt tggcaaatgc aacctcactt gcccgtggtg
     3541 cgataccaat tacaatcaat ttgagcaatg gacgttaagc gatattcttg ccaaagtaag
     3601 aagctattct gcgaaaaatg tgattattac cggaggcgag ccaacgattc agcctaattt
     3661 atcgttatta ttagaacaat ttaaaaaaga gggatatttt ttagcgatcg aaaccaacgg
     3721 cttaaaagag gttccggcac aaattgatta tattgcgacc agcccaaagc gaatgtacca
     3781 agaaaagtat caacgccgtt gcattgaatt tgcgaatgaa gtccgtatcg tcgttgatgg
     3841 agaagtacag ggtttctgtg agcaaataga aagccaaatt aaagctgagc attactatct
     3901 ttcaccttgt gaggtagagg gaaaaatgaa cttgctggaa accatcaccc agattgggtt
     3961 attaaatcag cgtccaaata agcctaaatg gcagctaagt atccaaacgc ataaaatcgt
     4021 ggggattgaa taagtagaag ttagaagatg attttctaca atttaaaaga ctgagccgat
     4081 attatcggac tcagtctttt ttacttcagt ttttagcaga agataacttt attgtttctc
     4141 gctaaccatt atagcttgcc agctctcgct ttttgcttca ccgattaagt atttttggtc
     4201 gccagctcct tttgcagaat caataaattt aatatccatt acagcttttt ccataccttg
     4261 actgtaactc tgtttacgtt catttaatat tacagatata acagggttat ctgctgttgg
     4321 agaaattact aaactttttg tatcaccagt gattgtaaag atcggtttta cggaattttc
     4381 atcacttata ttaccgtttg ccttaccatt tttgaattca atgttaacat ctcccagttt
     4441 atttaaacgt gcttcaaact cttttatacc tgcatttgca ccatatacaa cccctccttt
     4501 tttgtcataa gtggctgtta agtcttgaga tactttactg ttaacacgat tttcatcata
     4561 aataacaaaa tatttgctga atttatccgc ttcttctaca agaatattac tttgtgtatc
     4621 gtgataaacg ccatagaaca gatctccgct acgtcctatt agataatcag cgttttgatc
     4681 agatgttaat gctccggtag aaatatctgc caacggtagt atgatctggt tcttttcttt
     4741 aataattggt ttgccactaa cacgatcata ttttttgggg ctatcaggtt tatcaactag
     4801 aataaattta ttgagtgtaa tagtttgttt ttctgcatca agtattactg tcccaatttt
     4861 atcaaaagat ccatctattt tcttttcagg tattgacgga gcaatttgtg ttgctggagg
     4921 agcaacttct ggctgatcct gtatttggga attattttga ctttgggtgt tattttccat
     4981 ctgaggaaca ttttgtacct gaggaacatt ttgtacctga ggagcatttt gtacctgagg
     5041 agtattttgt gtttttggta ctagtactgg tttgggttgg ttcggtgttg aaggcgaacc
     5101 tccgctacta cttcctccgc aagcacttaa aactaaggtt gcggcaaatg ttaaaattaa
     5161 ttttttattg aatttcattg ggttattcct taaatataaa aacgagacta tgagaagtct
     5221 atcatatttt tttatatgaa ataagaatga atggtctgag gagttttacg taaacgtttc
     5281 cct
//
LOCUS       DANJWC010000155         5224 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.155, whole genome shotgun sequence.
ACCESSION   DANJWC010000155 DANJWC010000000
VERSION     DANJWC010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5224
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.155"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            77..2686
                     /gene="adhE"
                     /locus_tag="PWX02_002060"
                     /gene_synonym="adhC"
     CDS             77..2686
                     /gene="adhE"
                     /locus_tag="PWX02_002060"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDL1730034.1"
                     /translation="MAKNTQPFDAQAEVNELVEKGLKALDEFRKLNQEQVDFIVAKAS
                     VAALDKHGILAMHAYEETGRGVFEDKATKNLFACEYVVNNMRHLKTAGVISEDDVTGI
                     TEIADPVGVVCGITPTTNPTSTTIFKALIALKTRNPIVFAFHPSAQQSSAHAAQIVRD
                     AAVAAGAPENCIQWITQPSMEGTSALMKHPGIATILATGGNAMVEAAYSCGKPALGVG
                     AGNVPAYVEKTAKLKQAVYDIVMSKSFDNGMICASEQAAIVDKEIYAEFIKEMQSYGV
                     YLANKKEKALLEKYIFGIDKAKDENCSGAKLNSAVVGKPAAWIAEQAGFKVPEKTNIL
                     LAECAFVGEAEPLTREKLSPVLALLKSDSTEHGLELSEAMVNFHGLGHSAAIHTENKA
                     LAKTFGERVKAIRVIWNSPSTFGGIGDVYNSFLPSLTLGCGSYGKNSVGNNVSAVNLI
                     NIKRVGRRRNNMQWFKVPSKIYFERDSIQYLKSMHDVHKVMIVTDRSMVDLGFVDRIT
                     EQLRQRRNQVMIQLFTDVEPNPSLQTVQRGTELMRSFQPDTIIALGGGSPMDAAKIMW
                     LFYEQPEVDFRDLVQKFMDIRKRAFKFPQLGRKAKFVGIPTTSGTGSEVTPFAVITDG
                     DIKYPLADYSLTPTIAIVDPALVMSVPSHVAADTGLDVLTHATEAYTSILANDYTDGL
                     ALQAIKLVFENLEKSVKEFDETAREKMHNASTMAGMAFANAFLGICHSMAHKIGGKFH
                     TIHGRTNAILLPHVIRYNGTRPTKVATWPKYTNYVADVRFQDIARMLGLPAETPEQAV
                     EAYAQAVHDLAVRCGVKMSLREQGVDEQAFLNARRELALHAFEDQCTPANPRLAMIED
                     MEAIMTKAYYGKE"
     gene            2833..3729
                     /locus_tag="PWX02_002061"
     CDS             2833..3729
                     /locus_tag="PWX02_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HDL1730035.1"
                     /translation="MQITILDGGISRELMRRNAPFHQPEWSAAALYEGPHFVQAVHED
                     FIAHGAEVITTDSYAVVPFHIGEKRFAADGKMLADLAGRLAKQAVKNSENFATQIAGS
                     LPPMFGSYRADLIQMERFAEVAQPLIDGLSPYVDIWLCETQSAIIEPISIKALLPKDN
                     RPLWVSFTLTDDEPTTEPQLRSGESVKQAVEEMVKLGVQAILFNCCQPEVIGEALSVT
                     QKTLAELKATHIRTGAYANAFAPQPKDATANDGLDEVRQDLTLEAYLGWAKKWTEQGA
                     TIIGGCCGIGVEYIETLAKNLK"
     gene            3739..5148
                     /locus_tag="PWX02_002062"
     CDS             3739..5148
                     /locus_tag="PWX02_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1730036.1"
                     /translation="MSNKKIGLISLTALVLSSMIGSGIFSLPQNMAAVAGAEAITIGW
                     LITGVGIIFLGLSFFFISRLRPDLDGGIYTYAREGFGELMGFMSAWGYWLCATIGIVG
                     YLTVAFEGLGVFTDNPNTVIFGQGNTLASFIGSSIIVWLVHALIAGGIKEAAFVNLIA
                     TFVKVAPLILFIGLGIWYFDVDLFNSDVKATALNNSVGDQVKSTMLITLWVFTGVEGA
                     SVLSAHAKKRSDVGLATVLGIIIALALYVAITILSLGILPRETIANMANPSMGPLLDA
                     MMGPTGKVIITACLIVSVLASYISWTMYSAEVPYRGSKNGAFPKILDKVNKNGTPINS
                     LWFTGFIVQFCLVLVFVFEQSYNTLLLISTSMILIPYFLIGAYLFKLAIQTNATWYIK
                     LTGFMAALYGLWIVYAAGLEYLLLSVVLYVPGILLFLYSNYKFNGRLTLSTAEKVILI
                     VLLVIFGYAVIELPALLAA"
BASE COUNT         1451 a         1183 c         1146 g         1444 t
ORIGIN      
        1 acaaaataac tcttttgggt aataatttca tcagttaaaa aaattatcaa atttaaattt
       61 tatgaggaac caaactatgg ctaaaaatac gcaaccattt gatgcccaag cagaagtgaa
      121 tgagcttgtt gaaaaaggct taaaagccct tgatgagttt agaaaactca accaagaaca
      181 agtggatttt attgtcgcta aagcctctgt tgctgccctt gataaacacg gtattttagc
      241 aatgcacgct tacgaagaaa ccggacgggg tgtgtttgaa gacaaagcca ctaaaaacct
      301 gttcgcctgt gagtatgttg tcaataatat gcgacatttg aaaaccgcag gggtcattag
      361 tgaagatgat gtcaccggta ttaccgaaat tgccgaccct gtcggtgttg tctgcggtat
      421 caccccaacc accaacccaa cctcaaccac cattttcaaa gcgttaatcg cccttaaaac
      481 ccgtaaccct atcgtttttg ctttccaccc gtctgctcaa caatcttctg cccacgctgc
      541 tcaaattgtg cgtgatgccg ccgtggctgc cggtgcaccg gaaaactgta ttcaatggat
      601 tacccagcct tcaatggaag gtacatctgc tttaatgaaa cacccgggca ttgcaactat
      661 tttagccacc ggcggtaatg caatggttga agccgcttac tcttgcggca aaccggcatt
      721 aggagttggt gcaggcaacg taccggctta tgtagaaaaa accgctaaat taaaacaagc
      781 ggtgtatgac attgtaatgt caaaatcttt cgacaacggt atgatttgtg cctctgaaca
      841 agcggcgatt gtagataaag aaatttacgc tgagtttatt aaagagatgc aatcttacgg
      901 cgtctattta gcgaacaaaa aagagaaagc cttattggaa aaatacattt tcggtattga
      961 taaagccaaa gatgaaaact gctcaggtgc aaaactcaat tcagcggtgg ttggcaaacc
     1021 ggctgcgtgg attgcagaac aagccggctt taaagtaccg gaaaaaacca atattctgtt
     1081 agccgaatgt gcctttgtgg gcgaagctga gccacttacc cgtgaaaaac tttcgcctgt
     1141 actggctctg ttaaaatccg actccaccga acacggtttg gaattgtctg aagcgatggt
     1201 gaatttccac ggtttagggc atagtgccgc cattcatacc gaaaacaaag ccttagccaa
     1261 aacctttggc gaacgggtaa aagccattcg ggtgatttgg aactcaccat caacctttgg
     1321 cggtatcggt gatgtatata actccttcct gccatcgctt acgctgggct gtggttccta
     1381 cggtaaaaac tctgtaggta acaacgttag tgcggtaaat ttaatcaata tcaaacgtgt
     1441 gggcagacgg agaaataata tgcaatggtt taaagtgcca tcaaaaatct actttgagcg
     1501 tgactcaatt caatatttga aatcaatgca cgatgtgcat aaagtaatga tcgtaaccga
     1561 ccgttcaatg gttgatctcg gctttgtgga tagaattacc gaacaacttc gccaacgtcg
     1621 caaccaagtg atgattcagc tctttaccga tgttgaacca aacccaagcc tgcaaaccgt
     1681 acaacgtggt acagaattaa tgcgtagctt ccagcctgat actatcatcg cacttggtgg
     1741 tggttcacca atggatgcag ccaaaattat gtggttattc tacgaacaac cggaagtcga
     1801 tttccgtgat ttagtgcaaa aatttatgga tatccgcaaa cgtgcattca aattcccaca
     1861 attaggcaga aaagcgaaat ttgtcggcat tccaaccacc tccggaacag gctctgaagt
     1921 cacaccgttt gcagtaatta ccgatggcga tattaaatat ccgctcgctg actactcgct
     1981 taccccaacc attgcgattg tcgatcctgc gttagtaatg tctgtgccgt ctcacgttgc
     2041 tgccgacacc ggtttagacg tattaactca cgctaccgag gcttacacat cgattttagc
     2101 caacgactat accgacggct tagccttgca agccattaag ttagtgtttg aaaacttgga
     2161 aaaatcggtc aaagagtttg atgaaaccgc ccgtgaaaaa atgcacaatg cttccacaat
     2221 ggcagggatg gcatttgcca atgccttctt aggtatttgc cactcaatgg cacacaaaat
     2281 cgggggtaaa ttccacacca tccacggtcg aaccaatgcg attttactgc cacacgttat
     2341 tcgctataac ggcacacgcc caaccaaagt ggcaacttgg ccaaaataca ccaattatgt
     2401 cgcagatgtg cgtttccaag atattgccag aatgcttggc ttaccggcag aaaccccgga
     2461 acaagcggtg gaagcttacg cccaagcggt acacgattta gccgttcgtt gcggcgtgaa
     2521 aatgtcgctt cgtgagcaag gcgttgatga gcaagccttc ttgaatgccc gccgtgaact
     2581 tgccctacac gcatttgaag accaatgcac accagcaaac ccacgtttgg caatgattga
     2641 agatatggaa gcgattatga ctaaagccta ctatggcaag gaataaagca caatttaatc
     2701 gttaaactta ctaaatggcc gcttgcaagc ggtcattttt cttgaaattt ttacttttcc
     2761 cttcacaaaa tactttcgct cttttacaaa gcatagtatt tttatcgctc cttttatctt
     2821 acaggaaata ctatgcaaat tacgatttta gacggcggaa ttagccgtga attaatgcgt
     2881 cgcaatgctc catttcatca gcctgaatgg tctgctgccg ctttgtatga aggcccacat
     2941 tttgtgcaag cggtacacga agattttatc gctcacgggg cagaagtgat tactaccgac
     3001 agctatgcgg ttgtgccgtt tcacattggc gaaaaacgct ttgcggcaga cggcaaaatg
     3061 cttgccgatt tagccggtcg ccttgccaaa caagcggtca aaaattcgga aaatttcgca
     3121 actcaaattg caggctcact tccaccaatg tttggctctt accgtgccga tttaattcaa
     3181 atggaacgct ttgctgaagt cgctcagcct ttaattgacg ggctttcgcc ttatgtggat
     3241 atttggcttt gcgaaaccca aagtgcgatt attgagccga tttccatcaa agcattattg
     3301 ccgaaagaca accgcccgct ttgggtctct tttaccctca ccgatgatga accaaccacc
     3361 gagccacaac ttcgctcggg cgaaagcgtt aaacaagcag tcgaagaaat ggtgaagtta
     3421 ggcgtgcaag cgattttatt taactgctgt cagcctgaag tgatcggcga ggcattaagt
     3481 gtaacccaaa aaactttagc cgaattaaaa gctacccata ttcgcacagg agcttatgcc
     3541 aacgcctttg ctccacaacc aaaagatgcc accgccaatg acggtttaga tgaagttcgc
     3601 caagatttaa ccctggaggc ttatttaggc tgggcaaaaa aatggacgga acaaggggca
     3661 actattatcg gtggctgctg cggcattggg gttgagtata ttgaaacgct ggcaaaaaat
     3721 ttaaaataag gaaatcgaat gtcaaataaa aaaataggat taatttcact caccgcctta
     3781 gtgctaagct caatgattgg ctcggggatt ttcagcttac cgcaaaatat ggcagccgtt
     3841 gccggagcag aagccattac cattggctgg ctgattaccg gtgtcggcat tatcttttta
     3901 ggtttatcgt ttttctttat ttcccgcttg cgtccggatt tagacggtgg catttacacc
     3961 tacgcacggg agggttttgg cgagctgatg ggctttatgt cagcgtgggg ctattggctc
     4021 tgtgccacca ttgggattgt aggatacctc accgttgctt ttgaaggttt gggtgtattt
     4081 accgacaacc cgaacaccgt gattttcggg caaggtaaca ccctcgcctc ttttattgga
     4141 tcttcaatca ttgtgtggct ggttcacgcc ctcatcgcag gcggtatcaa agaagctgcc
     4201 tttgtaaact taattgccac ttttgtgaaa gtcgctccgc tcattctgtt tatcgggtta
     4261 ggcatttggt atttcgatgt ggatctgttt aatagcgatg ttaaagccac cgcacttaat
     4321 aatagtgtag gcgaccaagt gaaaagcaca atgttaatca ccttatgggt atttaccggt
     4381 gtggaagggg cttctgtgct ttcggctcac gcgaaaaagc gttctgacgt gggcctggca
     4441 accgtattag gtattattat tgcacttgct ctttatgtgg caattaccat tctttcactc
     4501 ggaattctgc cacgggaaac tattgccaat atggcaaatc cgtcaatggg gccgttgctc
     4561 gatgcaatga tgggcccaac cggcaaagtg attattactg cttgtttaat tgtatcggtg
     4621 cttgcctctt acattagctg gacgatgtat tcggcagaag tgccttatcg tggctcaaaa
     4681 aacggggctt tcccgaaaat tttagacaaa gtgaataaaa acggcacgcc gattaactca
     4741 ctctggttta ccggctttat cgtgcaattt tgcttggtgt tggtgtttgt ttttgagcaa
     4801 agctataaca ccttattgct gatttccacc tcgatgattt taattcccta tttcttaatc
     4861 ggggcttatt tattcaaatt agcgattcaa accaacgcca cttggtacat taaactcacc
     4921 ggctttatgg cagcccttta cggattatgg attgtctatg ccgcaggact agaatatttg
     4981 ctgctttcgg tggtgcttta tgtacccgga attttactct tcctctactc caactataaa
     5041 ttcaacggca gattaaccct ttcaaccgct gaaaaggtca tcttaatcgt gctactggta
     5101 atttttggct atgccgtgat tgagctgcca gcactattag cagcataatt cccatcaaaa
     5161 atgaccgctt gcaagcggtt atttttctag gatcttttgc aaagattcat tagaatagaa
     5221 aaaa
//
LOCUS       DANJWC010000156         5199 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.156, whole genome shotgun sequence.
ACCESSION   DANJWC010000156 DANJWC010000000
VERSION     DANJWC010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.156"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..321)
                     /locus_tag="PWX02_002063"
     CDS             complement(<1..321)
                     /locus_tag="PWX02_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1730037.1"
                     /translation="MAQALLYFTTKYLQSIEGYFMSIKFVDLLQQSWNFMRNQQAFSL
                     FAIVTIVVVQLVFILVSPNSPELMVESQPQQLQIDASKMVSILLPTILLGVVNLLINV
                     LMIFN"
     gene            392..829
                     /locus_tag="PWX02_002064"
     CDS             392..829
                     /locus_tag="PWX02_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HDL1730038.1"
                     /translation="MLSLFDAGQRYLNTLPNQKKLANFMPDYHIIRLVKFSSKVMPAF
                     ACFALVWQYCFTDPTQSILANSVLTAVFAISIPFQGLYWLGKRARSPLSLSLLEWYES
                     LRQKLISAQVKLEETQAVPSFQDFANLLKLAEETWGDEYFDEL"
     gene            complement(949..1024)
                     /locus_tag="PWX02_002065"
     tRNA            complement(949..1024)
                     /locus_tag="PWX02_002065"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(989..991),aa:Asn,seq:gtt)
     gene            complement(1140..1889)
                     /gene="moeB"
                     /locus_tag="PWX02_002066"
     CDS             complement(1140..1889)
                     /gene="moeB"
                     /locus_tag="PWX02_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HDL1730039.1"
                     /translation="MELTDHEMLRYNRQIILKSVDFDGQEKLKASRVLIVGLGGLGCA
                     ASQYLASAGVGHLTLVDFDLVSLSNLQRQILHTDATIGQPKVESAKARLAAINPNIQI
                     ETINQRCSDEQLAKLIANVDLVLDCTDNVTVRNQLNLQCFKQKRPLVSGSAIRFEGQV
                     SVFCYQEQEPCYECLSRLFGDATLSCVEAGVIAPIVGIIGSIQALEAIKVLLNIGRTL
                     SGKLLMIDGLHFSVREMALPKQNNCPVCTSY"
     gene            complement(1988..3217)
                     /gene="moeA"
                     /locus_tag="PWX02_002067"
     CDS             complement(1988..3217)
                     /gene="moeA"
                     /locus_tag="PWX02_002067"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDL1730040.1"
                     /translation="MALLALSDALDNMLARLSTPKLVEQLPLNECANRVLAEDIFSPI
                     NVPNFDNSAMDGYAVRISDLAQNLTLNVAGKAFAGNPFNGEIKAGECVRIMTGALVPQ
                     GVDAVVMQEETTLNSDGSVTFNHIPKLGSNIRRIGEDVKQGALVLKKGSLLNVASLPL
                     LASLGIASVPVFVKLKVAILSTGDELTSVGEPLSEGKIYDTNRFAVRLMLEKLNCEIL
                     DFGILPDDPILFEQTFRQAQQQADVLITSGGVSVGEADFTKDVLEKLGEIGFWKIAMK
                     PGKPFAFGKLEQAWFFGLPGNPVSALVTFYQLAQPALAKLSGLSTDKIAKLTQNLTAC
                     SAGKLKKAVGRQDFQRGYYYANEKGELEVRAVGTQGSHIFSAFNESNCFIVLEAERGN
                     VDAGEKVTIQPFNSLMY"
     gene            complement(3239..3856)
                     /locus_tag="PWX02_002068"
     CDS             complement(3239..3856)
                     /locus_tag="PWX02_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006719828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HDL1730041.1"
                     /translation="MFDSLAVQFVVLWAVIDPIGSVPVYLAKTIGLPIEARRKIALNA
                     VLIAAGILIFFLIGGQVLLESMQIPLPAFQAAGGLVLLLFALTMIFGESKPEQEMRIS
                     SNLNELAVYPLAVPSIASPGAMMAIVLLTDNHRFHIGDQIITAGIMLLVLFITYLLLL
                     AANRIQHLIGNAGAAIISRVMGLILAAIAVNNLLIGVRDFFVQIS"
     gene            complement(3924..5135)
                     /locus_tag="PWX02_002069"
     CDS             complement(3924..5135)
                     /locus_tag="PWX02_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL1730042.1"
                     /translation="MISRQMQSLLKRLVAQFPAVLVTGARQVGKSTLLQHIGEGYQYI
                     TFDDPMLLELAKQKSRLFFLNHQGKLILDEVQYVPEIFALLKLEIDKKQQNGLFLLSG
                     SQAFELMQNVSETLAGRIAILKLQGLSLREILGVKFDQPFLPTTDYLLAREQELKSPE
                     NIWQLIHKGDMPRLYAQEVDWEIYYRSYVATYIERDVRQLTNIASTTDFTRFMVSIAS
                     RSGELLNYSNIAQEVGVSNETIKRWVAILQTSGIIYLLQPYHNNHLKRAIKTPKVYFL
                     DTGLMAYLTKWLTPETIAQGAKSWQFFETFVVSQIIKSFHNQGIEPPIYFYRDTNQKE
                     IDLIIEYDRTLYPIEIKVTASPTKKMAKSFDLLNSVSSEWKLGQGAIINQYPTKLYLS
                     ENLVALPVSYL"
BASE COUNT         1623 a         1151 c          946 g         1479 t
ORIGIN      
        1 attgaaaatc attaagacat tgatcaaaag attgacaaca cctaataaaa tagttggcaa
       61 tagtatagac accattttag aggcatcaat ttgaagttgt tgcggttgcg actccaccat
      121 tagctcaggc gaatttggcg acactaaaat aaaaaccaac tgcacaacca caatggtgac
      181 aatggcaaac agtgaaaatg cttgttgatt acgcataaag ttccaacttt gctgcaataa
      241 atcgacaaat ttaattgaca taaaataccc ttcaatagat tgtaaatatt ttgttgtgaa
      301 gtatagcaag gcttgagcca aactgcatta acctattgta gattttaatc agttagctaa
      361 aataaaagaa aaatttgaaa gagataatcc tatgttaagc ttatttgatg caggacagcg
      421 ttacctcaac actctgccga atcagaaaaa attagccaat tttatgccag attaccacat
      481 tattcgcttg gtaaaatttt cctctaaagt aatgcctgct tttgcttgct ttgcattagt
      541 ttggcaatat tgctttaccg atccgaccca atctatttta gccaattcgg tacttactgc
      601 tgttttcgcc atttctattc cgtttcaagg gttatattgg cttggaaaac gggcaagatc
      661 accgttatct ttatcattat tggaatggta tgagtcgctt agacaaaaat taatttcggc
      721 acaagtcaaa ttagaagaaa cacaagcggt accttctttc caagattttg caaatttact
      781 caagctagca gaagagactt ggggagatga atattttgat gagttataaa ataaaaaaag
      841 ccccacttaa aataagtggg gtaaaacttt tgttctaatt ctggaggttt gaattactgt
      901 tttataaaag gagacaaata aaacaagaac aaaagaaaaa taaaatcttg gctcctcctg
      961 cgggactcga acctgcgaca tatggattaa cagtccaccg ttctaccgac tgaactaagg
     1021 aggaattaac ggtgtaaata ataggctaaa acgttttagc cgtcaaataa aaaaaataaa
     1081 ctgtcgttca ttttttacat ttcttgacat tttttcgcca ataagtatga gaaaaatact
     1141 taataagagg tacaaaccgg acagttattt tgcttaggta atgccatttc tctgacagaa
     1201 aaatgcaagc cgtcaatcat taacaatttt ccggaaagag ttctgccgat attaagtagc
     1261 actttaattg cttccaacgc ttgaatactg cctatgatgc caacaatcgg agcaatgacc
     1321 cccgcttcaa cacaacttaa agtcgcatca ccaaacaacc tgcttaaaca ttcatagcac
     1381 ggttcttgtt cttgataaca aaatacactc acctgccctt caaatcgaat tgccgaaccg
     1441 gaaacaagcg gtcgtttttg cttaaaacat tgcaaattta actgattacg cacagtaacg
     1501 ttatcggtac aatccagcac taaatctaca tttgcaatca gcttggctaa ctgctcatcg
     1561 gaacatcgct gattgatagt ttcaatctga atgttcggat taatcgccgc taatcgagct
     1621 ttggcagatt ccacctttgg ctgcccgata gtcgcatcgg tatgtaaaat ttggcgttgc
     1681 agattagaaa gtgagaccag atcaaaatcc accaaagtca aatgcccaac gccggcagaa
     1741 gctaagtatt gcgatgccgc acagcccaaa ccacctaaac cgacaatcaa cactcggctg
     1801 gcttttaact tttcctgccc gtcaaaatcc acgcttttta ggataatctg gcgattataa
     1861 cgtagcattt cgtgatcggt aagttccatt tttacaccta aagagtaaac gatggcgcaa
     1921 gcgtccacgc ttgtgcctat atttttattg ctagcacaag cgtggacgct tgcgctatct
     1981 taattcatta atacatcagc gaattaaacg gctgaatagt gactttttcc ccagcatcaa
     2041 cattgcctcg ttctgcttct agcacaataa aacagttact ttcattaaaa gcgctgaaaa
     2101 tatgtgagcc ttgcgtgcca actgcacgaa cttctaactc acctttttca ttggcatagt
     2161 aatagccccg ttggaaatct tggcgaccga ccgctttttt caattttccg gcactgcaag
     2221 cggttagatt ttgggttaat tttgcaattt tatctgtcga taaacccgaa agtttggcta
     2281 acgccggttg cgctaattgg tagaaagtca ctaatgcaga gacagggttg cccggcagcc
     2341 caaaaaacca agcctgttct aatttaccga atgcaaaagg cttgcccggc ttcatcgcaa
     2401 ttttccagaa accgatttcg cctaattttt ccagtacatc tttggtaaaa tctgcctctc
     2461 ccacagatac gccgccactg gtaatcaaca catcggcttg ctgttgggct tgcctgaaag
     2521 tttgctcaaa cagaattggg tcatcaggca aaatcccaaa atctaaaatc tcgcagttga
     2581 gtttttccag cattaaacgc accgcaaaac ggttggtgtc gtaaattttt ccctcactca
     2641 aaggctcgcc aactgaggtt aattcatcgc cggttgaaag aattgccact tttaatttta
     2701 caaaaacagg cacagaagca ataccgagtg aagctagcaa cggtaaactg gctacattca
     2761 ataatgagcc ttttttcaag accaatgccc cctgtttaac atcttcccca atgcggcgaa
     2821 tgttactgcc taattttgga atatggttaa aggttacaga gccatcggaa tttaacgtag
     2881 tttcctcttg cattacaacg gcatcaacgc cctgtggcac taatgctccc gtcataatcc
     2941 gcacgcattc gcctgccttt atttcgccgt taaacgggtt accggcaaag gctttaccag
     3001 ccacattcaa ggttaaattt tgtgctaaat ctgaaatacg aaccgcgtag ccgtccatcg
     3061 ccgaattatc aaaattcggt acattaatcg gggaaaaaat atcctctgcc aacactcggt
     3121 tagcacactc atttaacgga agttgttcca ccaatttagg tgtcgataaa cgagcaagca
     3181 tattatctaa cgcatcactc aaagccaaaa gtgccataat ttcctaccta ttcaacgttt
     3241 agctaatttg tacaaagaaa tcccgtacgc caatcaacag attattcacc gcaatcgcag
     3301 ccagaattaa ccccatcact cggctgataa tcgcagcccc tgcattacca ataaggtgct
     3361 gaatacggtt tgccgctaat agcaacaaat aggtaataaa gagtaccaaa agcataatgc
     3421 cggcggtgat aatttgatcg ccgatatgaa aacggtgatt gtcggttaat aatacgattg
     3481 ccatcatcgc cccgggagaa gcaatcgacg gtacagctag cggataaacc gccaattcat
     3541 tcagattact gctgatccgc atttcttgct cgggcttgct ttcgccaaaa atcatcgtta
     3601 aagcaaataa caacagcact aagccgcctg ccgcttggaa tgcaggtaac ggaatctgca
     3661 ttgattctaa cagcacttgc ccaccgataa ggaagaaaat caaaataccg gcggcaatca
     3721 agaccgcatt tagtgcaatt ttacgccgtg cttcaatcgg caaaccaatg gtttttgcca
     3781 aataaacagg aacagaacca atcgggtcga tcaccgccca taaaaccaca aattgtaccg
     3841 ctaatgaatc aaacatattc tctcctgtta tcttgataat ttgcaaaatt ttagcataaa
     3901 acaaccgctt gcacggcttt ttcttacaga tagctcaccg gtaacgctac caaattttca
     3961 cttaaataaa gtttggtcgg atattggtta ataatcgctc cctgccctaa tttccattca
     4021 ctagaaactg agtttagcaa gtcaaaactt tttgccattt ttttggttgg gcttgccgtg
     4081 accttaattt ctatcggata aagcgtgcga tcatattcaa taattaagtc gatctctttc
     4141 tggttagtat cacggtaaaa ataaatcggc ggctcaattc cttgattatg aaaggatttt
     4201 ataatttggc ttaccacaaa ggtttcaaag aactgccagc tttttgcccc ttgagcaata
     4261 gtttcggggg taagccattt ggttaaatac gccattaacc cggtgtctag aaaatacact
     4321 tttggggtct taattgcccg tttcaaatga ttgttgtggt atggctggag caaatagata
     4381 atgccggaag tctgcaaaat ggcgacccaa cgtttaatcg tttcattcga tactccaact
     4441 tcttgagcaa tattactgta attgagtagc tcaccactac gagaggcaat agagaccata
     4501 aaacgggtaa aatccgttgt gctggcaata ttggttaatt ggcgaacatc tcgctcaatg
     4561 taggtcgcta cataagagcg ataataaatc tcccaatcca cctcttgtgc atataaacga
     4621 ggcatatcgc ctttatggat aagctgccag atgttctccg gtgattttaa ttcttgctcc
     4681 cgagctaaca aatagtcggt tgtcggtaaa aaaggttgat cgaacttcac ccctaaaatt
     4741 tcacgcaacg aaagcccttg taattttaaa atagcaatac gaccggcaag tgtttcactc
     4801 acattctgca ttaattcaaa ggcttgcgac cccgaaagta aaaacaaacc gttttgctgt
     4861 tttttatcaa tttctaactt taataacgca aaaatttcag gcacatactg tacttcgtct
     4921 aaaataagct tgccttgatg gtttaaaaaa aacaggcgag atttttgttt agccagctcc
     4981 agcaacattg ggtcatcaaa agtaatatat tgatagccct caccaatatg ttgtaacaat
     5041 gtcgatttac caacctgtct tgcccctgtc actaaaacgg caggaaattg cgccacgagt
     5101 cgttttaaca gcgattgcat ttgccgagaa atcatttttc ctccaaaata gcctaaatca
     5161 agtttaacct catatataat gttgaattaa acttaattt
//
LOCUS       DANJWC010000157         5188 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.157, whole genome shotgun sequence.
ACCESSION   DANJWC010000157 DANJWC010000000
VERSION     DANJWC010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5188
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.157"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            115..2943
                     /locus_tag="PWX02_002070"
     CDS             115..2943
                     /locus_tag="PWX02_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDL1730043.1"
                     /translation="MYKIKHSFNKTLIAISISSFLSIAYATESIKNPQPIIQLSESLS
                     SKYSGKGVKLGVMDEGFMVKHPRHSSHLHPLIHQLTTPEGEVRIYDASYPQFEVNPVE
                     KEEEKEEEKEDGIDLIPSLETHGAGVAGIIAAQADKTLGDGYSGGIAKGAELYVATKS
                     YKRTLEKVIQDAKKELENAKDEEDEKTPSLDQMAKNDLLASKEKEMAIERAEWASGLN
                     KLLDNNVFAINNSWNPFSISDDINVVDKFYQSIKQNKHNPLLQAIMRAKNSNTLLVFA
                     AGNESKKQPGVMALLPRYFPELEKNLISAVAVDKEQKIAPYSNHCGASKNWCVAAPGD
                     LHVLIGVADEHKKPQYGLTKEQGTSFSAPAITASLAVLKERFDYLTATQIRDTLLTTA
                     TDLGEKGVDNVYGWGLINLKKAVNGPTQFLNDETITVTRDDHWSNPLASQFKITKKGD
                     KSLHLDGENHLDTVAVEEGRLALNGKTKVKTISNHANLAVNGTEVEQNYSSSGQSQLE
                     VLGKSGLIANAQANIHLAGSLKIDDKLTEKTEAGDVSATIVQLKDKATYQGGFTQLVE
                     NENLAKRGLIQDLYFKESEIIAKVNKPLTDEKADTNGQAGLALLNALRTTPIAYRRSW
                     YNGWLQSALEQRKLDNLHYAVSNNIYADSLELLRSQNAKGLTQAQQHLFTAYHTPLQT
                     TVWAEHLNQKQSASSKHTDVKHHQSQLGVNHKLADKTVLSATLSQQKNRLEKPFAQAT
                     LKQTALNIGLRYHLDNAWFSEATLQFARQKYQQSRRFASHQLGTAETRGSTLGGEMRI
                     GYQFMPNQWIIEPSLGVQWIQTKMNGLNESGELATQTAAMRYRDVNIVPSVKLQRTFQ
                     LEQGSISPYIGLNYLHRLNVKITKITSNIAGKTLHSEATTKRNRQLNGEVGVKLHYKN
                     WFTAMNLDYSRVKSANLFGWKVNVGFSF"
     gene            complement(3084..3521)
                     /locus_tag="PWX02_002071"
     CDS             complement(3084..3521)
                     /locus_tag="PWX02_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDL1730044.1"
                     /translation="MDWFFNWIGWSILGFVLLALELVLPGVFIMWWGFASLILAFIVA
                     LFTLGQALQIALFAIIATIFSLIWWKYQHGKDKKEDQSSSLNSREHAMLGAQGFVVEI
                     LPNGIARGKFGDTTWRVIGNNLQVADKIEVQKVEGITLFVRKQ"
     gene            complement(3557..4477)
                     /locus_tag="PWX02_002072"
     CDS             complement(3557..4477)
                     /locus_tag="PWX02_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDL1730045.1"
                     /translation="MNFDLPIVSIAFVVLVLVALSSTIKIVPQGYHWTVERFGRYTKT
                     LSPGLNIVVPFIDRIGRKMNMMEQVLDIPSQEVISRDNASVAIDAVCFVQTVDARRAA
                     YEVNHLEQAIVNLTMTNMRTVLGSMDLDDMLSQRDLINGRLLSIVDEATNIWGVKVTR
                     IEIRDVRPPKELVAAMNAQMKAERNKRADILEAEGIRQAEILRAEGEKQSRILKAEGE
                     RQEAFLQAEARERAAEAEAKATQMVSEAIAKGDTTAINYFIAQKYTEALKEIGSSDNS
                     KVVLMPLEAGNLIGSVAGIAEILKSNKTSS"
     gene            complement(4493..5182)
                     /gene="ung"
                     /locus_tag="PWX02_002073"
     CDS             complement(4493..5182)
                     /gene="ung"
                     /locus_tag="PWX02_002073"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL1730046.1"
                     /translation="MKTWTEALGEEKQKAYFQQILQYVHNERLAGKVIYPPQNEVFSA
                     FALTEFSDVKVVILGQDPYHGPNQAHGLAFSVKPPVAPPPSLVNMYKELSEDIEGFQI
                     PPHGYLIDWAKQGVFMLNTSLTVEQGRAGSHSQIGWEIFTDKVIAQLNQHRDNLVFLL
                     WGSHAQRKGQFIDRNRHCVLASPHPSPLSAYRGFFGCKHFSKANNYLISQGISPINWQ
                     LSVDVSYSSTH"
BASE COUNT         1753 a         1031 c          950 g         1454 t
ORIGIN      
        1 aaaattagaa aactaaatat atcagaaagt ttgatcttta tcattaacta gattataatt
       61 atttgtaata aaaaattaac tacgtaaaat aagcttattt ttggaagtaa aattatgtat
      121 aaaataaagc attcatttaa caaaacactt attgcaatta gcatttctag ttttttatct
      181 attgcatacg ctacagaatc aataaagaat ccacaaccta tcattcaatt atctgagagc
      241 ctcagctcaa aatatagtgg caagggtgtt aaactaggcg taatggatga gggcttcatg
      301 gttaaacacc ctcgccatag ttcacatctt catcctctta ttcatcaatt aacaacaccc
      361 gaaggcgaag taagaatcta cgatgcaagt tatcctcaat tcgaggtaaa ccccgtagaa
      421 aaagaagaag aaaaagaaga agaaaaagaa gatggaattg atcttattcc ttctcttgaa
      481 acacacggtg caggtgtagc tggtattatt gccgctcaag cagataaaac tcttggtgat
      541 ggctatagtg gtggtattgc aaagggagct gagctttatg ttgcaacgaa gtcatataaa
      601 cgaactttag aaaaagttat tcaagatgct aagaaagaat tagaaaatgc aaaagacgaa
      661 gaggatgaaa aaacgccaag tctggatcaa atggcaaaga atgacttatt agcaagcaaa
      721 gaaaaagaaa tggcaattga acgtgctgaa tgggcatccg ggctgaataa attactagat
      781 aataatgttt ttgcaatcaa taatagttgg aatccttttt ctattagcga tgacatcaat
      841 gttgtagata aattttatca atctatcaag caaaataagc acaatccgct acttcaagct
      901 attatgagag ccaaaaatag caacacctta ttagtctttg ctgctggtaa tgagagcaag
      961 aaacagccag gcgtaatggc tcttctacca cgttactttc ccgagcttga gaaaaattta
     1021 atttctgccg ttgcggtaga taaagaacaa aaaattgctc catactctaa ccactgcgga
     1081 gcaagtaaaa actggtgtgt agcagcaccg ggcgatttac atgttttaat tggcgttgct
     1141 gatgaacata aaaaaccaca atatggctta acaaaagagc aaggtacgtc attttcagca
     1201 cctgctataa ctgcttcatt agcagtatta aaagaacgct ttgattatct gactgcgact
     1261 caaattcgcg atactttatt gactaccgcc actgatttag gtgaaaaagg ggtcgataat
     1321 gtttatggct ggggtctcat taacctgaaa aaagctgtta atggcccaac tcaattctta
     1381 aatgatgaaa cgattaccgt aactcgtgat gaccattggt cgaatccact cgccagccaa
     1441 tttaaaatta caaaaaaagg ggacaaatca ctgcatttag acggcgaaaa tcatttagat
     1501 acggtagcag ttgaagaagg tagattggcc ttaaacggta aaactaaggt gaaaacaatc
     1561 agtaaccatg caaatttagc tgtcaatgga accgaagttg agcaaaatta ttcttcgtct
     1621 ggacaaagcc aattagaagt gttgggcaaa tctgggctaa ttgccaatgc tcaagccaat
     1681 atacatttag ccggctcgtt aaaaattgat gataaattaa cagaaaaaac cgaagcaggt
     1741 gatgtttcag cgaccatagt acagttgaaa gataaagcaa cttatcaagg cggatttaca
     1801 caattagtcg aaaacgagaa tttagccaaa cgagggttaa tccaagatct ctatttcaaa
     1861 gagagcgaaa ttatcgcaaa agtgaataag ccactaactg atgagaaagc cgatacaaac
     1921 gggcaagccg gtttagcatt attaaatgcg ttaagaacta cacctattgc ctatcgccgt
     1981 agttggtata acggttggct gcaatctgca ctcgaacaac gtaaattaga taatctgcac
     2041 tatgctgtat ctaacaatat ttatgcagat agccttgaat tgctacgtag ccaaaatgcc
     2101 aaaggcttaa cccaagcaca gcaacatttg tttacggcat accatacacc actgcaaacg
     2161 actgtttggg ctgaacattt gaatcaaaaa caatcagcct catcgaagca taccgatgtt
     2221 aaacaccacc aatcacaatt gggagtaaac cacaaactgg cagataaaac ggtacttagt
     2281 gcaacacttt cacagcaaaa aaaccgctta gaaaaaccat ttgcacaagc taccttaaaa
     2341 caaacagcat taaatattgg cttacgttat catttagata acgcttggtt tagtgaagct
     2401 acattgcaat ttgctcgcca gaaatatcaa caaagccgtc gatttgctag ccatcaacta
     2461 ggtactgcag aaaccagagg ttcaacgctc ggtggtgaaa tgcggatcgg ttatcaattt
     2521 atgccaaacc aatggataat tgaaccaagc cttggcgtac aatggattca aaccaaaatg
     2581 aatggtttaa atgaaagtgg cgaacttgcg actcaaacgg ctgcaatgcg ttatcgtgat
     2641 gtgaatattg ttccaagcgt gaaattacag cgaactttcc aacttgagca aggctctatt
     2701 tccccttata tcgggctaaa ttacttgcat agattaaatg taaaaataac caaaattacc
     2761 agtaacattg caggaaaaac actgcatagt gaagcaacga cgaaacgtaa tcgccaatta
     2821 aacggcgaag tcggggtgaa actgcattat aaaaattggt ttactgcgat gaatttagat
     2881 tacagtcgag tgaaatctgc aaacctattt ggttggaaag taaatgttgg ctttagtttc
     2941 taacttatta tggtgggaca caagcggtta atttagggtg aaaatttgca aatttcactc
     3001 tattttcaac cgcttgagag aaaaaacctt gaacaactgt tcaaggtttt tgtttagtgt
     3061 tctatgacta aataagaaaa aacttattgc ttacggacaa ataaggtaat tccctccact
     3121 ttctggactt caatcttatc tgctacttgt aaattattac ctataacacg ccaagtcgta
     3181 tcaccaaatt ttcctcgagc gatgccatta ggtaagattt caaccacaaa gccttgagct
     3241 cctagcatag catgctctcg agagttaagg gaactgctct gatcctcttt cttgtctttt
     3301 ccatgctgat atttccacca aataagacta aaaatagtcg caattatcgc aaataacgca
     3361 atttgtaacg cttgccctaa tgtaaaaagt gcaacgatga aagcaagaat aagcgaggca
     3421 aatccccacc acataataaa gacacccggt aatactaatt ctaatgcaag tagtacaaag
     3481 cctaatatgc tccagccaat ccaattaaaa aaccagtcca tactccctcc atatactcaa
     3541 ttaatccggt tttaggctat gagcttgttt tattgctttt taaaatttca gcaataccag
     3601 caacagaacc aatcaaattt ccagcttcta acggcataag tacaacttta ctgttatctg
     3661 aagaacctat ttctttcaaa gcttcggtgt atttttgtgc aataaaatag ttaatcgctg
     3721 tagtatcgcc tttagcaatt gcttcagaaa ccatttgagt ggctttagct tccgcttctg
     3781 cggctcgttc acgagcttct gcctgtaaga atgcttcttg tctttcacct tctgctttta
     3841 aaatacgaga ttgtttttcc ccttcagccc gcagaatttc agcttgtcga atcccttctg
     3901 cttcaagaat atcagcacgt ttattacgct cagctttcat ttgagcgttc attgccgcaa
     3961 ccagctcttt cggtggacga acatccctaa tctcaatacg ggtgactttt actccccaaa
     4021 tattagtcgc ctcatcgaca atggaaagta atctaccgtt aattaaatca cgctgagaga
     4081 gcatatcatc tagatccata gagccgagca cggtacgcat atttgtcatg gttaaattaa
     4141 caattgcttg ctctaaatga ttcacttcat aggcagctcg gcgagcatca actgtttgaa
     4201 caaaacatac agcatcaatc gcaacacttg cattatcacg tgaaataacc tcttgcgatg
     4261 gaatatctaa aacttgctcc atcatattca tttttcgccc aatacgatca ataaaaggta
     4321 cgacaatgtt caaccccggg cttagcgttt tagtataacg accaaaacgt tctactgtcc
     4381 aatgatagcc ctgtggcaca attttaatag tcgaacttaa tgcaactaaa acaagcacaa
     4441 caaatgcaat actaacaatc ggtaaatcaa aattcatcgt ttgctcctta aactaatgtg
     4501 ttgaagaata actaacgtct actgataatt gccaattaat tggggatatt ccttgagaaa
     4561 tcaagtagtt attggcttta gaaaaatgtt tacaaccaaa aaatccacga tatgcggaaa
     4621 gtggtgatgg gtgcggagaa gctaacacac aatggcgatt acgatcgata aattgcccct
     4681 ttctctgagc gtggcttccc caaagtagaa agactaaatt atcacggtgt tgatttaatt
     4741 gtgcgatcac tttatcggta aaaatttccc aaccaatttg tgaatgcgaa ccggctctac
     4801 cttgctcaac cgttaagcta gtgtttaaca taaatacccc ttgttttgcc cagtcaatta
     4861 aatagccatg cggtggaatt tgaaatcctt caatatcttc agataattct ttatacatat
     4921 tgaccagaga aggtggcggg gcaaccggcg gtttaactga aaaggctaat ccgtgggctt
     4981 gatttgggcc gtgataagga tcttgcccta aaattactac tttcacgtca ctaaattcgg
     5041 ttaaggcaaa agcactaaac acctcgtttt gcggaggata gatgactttg ccggctaatc
     5101 gttcgttatg tacatattgt aaaatttgtt gaaaatacgc tttttgtttt tcttcgccta
     5161 aagcttctgt ccaagttttc ataaaacc
//
LOCUS       DANJWC010000158         5184 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.158, whole genome shotgun sequence.
ACCESSION   DANJWC010000158 DANJWC010000000
VERSION     DANJWC010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.158"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..1780
                     /locus_tag="PWX02_002074"
     CDS             <1..1780
                     /locus_tag="PWX02_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDL1730047.1"
                     /translation="TLVLCLPSTLMFLAGFIAITYISFENPQEVWTWGLKMAIYWLYF
                     AFLVAMLRPHGIGHIHFNMPQKSNEIFRNILKRSVWIIALLLNTSIFTNLEMGISYDM
                     LGQTMTIFVLILMIWMIVPAFRQAISTYENVTTEEKKESKSILLTIVKLVLLLAPIIL
                     IILIALGYYYTALVIIEHLVDSYFAIITWIIIRNILYRAFSISSRRLAYRRLQEKREA
                     AEAKARAKAEQNDENPDGVELPFELKEDSIAVSEVKNQMLKLTDFVLWIGLFVLLYWV
                     WSDLITVALYLDGVTLWQQSVTTEAGIVMESITLLNLLTAILIIVVMVVLIRNLSGLL
                     EALIFSHFKLSQGTPYTITTLLTYLIIALGAIFAFGTLGMSWSKLQWLLTALSVGLGF
                     GMQEIFANFVSGIIILFERPMRIGDTITIGTYSGTVSKIRIRATTLVDFDGKEVIVPN
                     KAFVTERLVNWALSSTATRIIVQVGVAYGSDLELVKRLLLQVAEENPKVLKDPQPRAY
                     FLTFGASTLDHELRAYVNDLSDRNPTIDALNTRIHQLFNEHNIDIAFNQLDVYIKNND
                     TQDEIKVDSKRFEFERPNNITAKEKE"
     gene            1783..2865
                     /gene="aroC"
                     /locus_tag="PWX02_002075"
     CDS             1783..2865
                     /gene="aroC"
                     /locus_tag="PWX02_002075"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDL1730048.1"
                     /translation="MAGNSIGQLFKVTTFGESHGIALGCIVDGVPPNMALSEADLQPD
                     LDRRKPGTSRYTTPRREDDEVQILSGVFEGKTTGTSIGLIIKNGDQRSQDYGDIAEKF
                     RPGHADYTYQQKYGIRDYRGGGRSSARETAMRVAAGAIAKKYLREQFGIEVRGYLSQI
                     GNIKIDPKTVENIEQIDWHAVNSNPFFCPDPVAIEGFDALIRELKKDGDSVGAKLTVI
                     AERVPVGLGEPVFDRLDADLAHALMSINAVKAVEIGDGFAVVEQRGSQHRDEMTPQGF
                     LSNHAGGILGGISSGQPIIAHIALKPTSSITIPGRSVNKNNEAVEVITKGRHDPCVGI
                     RAVPIAEAMMAIVLLDHLLRFKAQCR"
     gene            2884..3711
                     /gene="mepA"
                     /locus_tag="PWX02_002076"
     CDS             2884..3711
                     /gene="mepA"
                     /locus_tag="PWX02_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HDL1730049.1"
                     /translation="MKLLKTLLVSSLAFTATALNATQWEKIKTPVQGKAQSIGGYSNG
                     CIIGAQPLALKGEGYQVIRSARNRYYGHPQLLDYLTYLGKKTKAAGVPTILIGDMGMP
                     AGGRFASGHASHQTGLDADIWFKFGPMDDETARNPAGLATIMVNRSTERVDDSLWNQH
                     HTNLVKLAATDPRVDRIFVNPAIKVKLCQTAGNDRAWLRKIRPWYGHDSHFHVRLTCP
                     ADAASCENQAPVPAGDGCGEELYSWFEPKEASGTPKAKVLPTAPVQCQMILSEQGLN"
     gene            3784..>5184
                     /locus_tag="PWX02_002077"
     CDS             3784..>5184
                     /locus_tag="PWX02_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HDL1730050.1"
                     /translation="MRLFVAEKPSLARAIADVLPKPHTKGDGFIQCGEHDTVTWCIGH
                     LLEQAEPDVYDERFKMWRMEHLPIVPEQWKLIPKKETLKQFKVVEKLIKKADVLVNAG
                     DPDREGQLLVDEVFGYLNLSAERKSQIQRCLVSDLNPNVVSRAIEKLQKNSDFIPLAT
                     SALARARADWLYGINMTRAYTLQGRWAGYKGVLSVGRVQTPVLGLIVRRDLEIEHFVP
                     KDYFEVLAQIVVPETQERFKAQWQPSKACEDYQDEDGRVLSRPLAENVVKRITDQPAT
                     VTEYQNKIEKETAPLPYSLSVLQMEAAGRYGLSAQAVLDICQKLYETHKLITYPRSDN
                     RYLPTEHFNDRFKVMAAIAHHLPEYQQKPEVIDATIRNRCWNDKEVEAHHAIIPTARQ
                     GTVHLTENEKRVYQLIARQYLMQFCPDAEYRKSKIGLEIAGGSFVAQARNLVIAGWRA
                     LLGKEDSLDDAEPLLPI"
BASE COUNT         1486 a         1142 c         1176 g         1380 t
ORIGIN      
        1 gacattagtg ctttgcctgc caagtacact aatgttctta gccggattta ttgctatcac
       61 ttatatttca tttgaaaatc ctcaggaagt ctggacttgg ggattaaaaa tggcaattta
      121 ttggctgtat tttgcctttt tagtggcgat gctacgcccg cacggcatag gacatattca
      181 ctttaatatg ccacaaaaaa gtaatgaaat cttccgtaat attttaaaac gttcggtatg
      241 gatcattgct ttattactga acacctctat ttttacaaac ttagagatgg gaatatctta
      301 cgacatgctt ggacaaacaa tgactatttt tgtccttata ttaatgattt ggatgattgt
      361 accggcattt aggcaagcta tttccaccta tgagaatgtc acaacagaag agaaaaaaga
      421 atctaaaagt attttactca ctattgtcaa attagtgcta ctgcttgccc ctatcattct
      481 tatcattctg attgcattgg gctattacta tactgcactg gtgatcattg agcatttagt
      541 agattcttat tttgcgatca ttacttggat tattattcgt aatattctct atcgagcatt
      601 ctctatttca tcacgccgtc tggcttacag acgtttacaa gaaaaaagag aagcagctga
      661 agccaaagca cgagctaaag cagagcaaaa tgatgaaaac ccggacggtg tcgagcttcc
      721 ttttgagcta aaagaagact ctattgccgt aagcgaagtc aagaaccaaa tgttgaaatt
      781 aactgatttt gtgctttgga ttggcttatt tgtgctgctt tactgggttt ggtcggactt
      841 aattactgtt gcactctacc tagacggcgt taccttatgg caacaaagtg ttacaacaga
      901 agccgggatt gtaatggaat ccattaccct gctgaattta ctcactgcta ttctgattat
      961 tgtggtaatg gtggtactca ttcggaactt atccggttta cttgaagccc tgattttctc
     1021 gcacttcaag ttatcgcaag gtacaccata cacaattacc acactcttaa cctatttgat
     1081 tattgcgtta ggggcaatct ttgcgttcgg tacattaggg atgtcttgga gcaagctcca
     1141 atggctacta actgcacttt ctgtcggtct gggttttggt atgcaagaga tctttgcaaa
     1201 cttcgtttca gggattatca tcttgtttga acgtccaatg cggattggcg acaccattac
     1261 catcggcact tacagcggta cggtttccaa aattcgtatt cgtgccacaa ccttagtcga
     1321 ttttgacggc aaagaagtga ttgtaccaaa caaagcattc gtaaccgaac gcttggtgaa
     1381 ctgggcatta agcagcaccg caacccgcat tattgttcaa gtcggcgtgg cttacgggtc
     1441 tgatttagaa ttagtcaaac ggctgttatt acaagtggcg gaagaaaatc cgaaagtctt
     1501 gaaagatccg caaccaaggg cttatttcct tacctttggt gcaagcactt tagatcacga
     1561 attaagagcc tatgtgaacg atttatctga tcgtaaccca actattgatg cactaaatac
     1621 acgcatccac cagctcttta atgaacataa tattgatatt gcatttaacc aattagatgt
     1681 ttatatcaaa aacaacgata cacaagatga aattaaagta gattcaaaac gctttgagtt
     1741 tgaacgacca aataacataa cagcaaaaga aaaggaataa caatggcagg caacagcatc
     1801 ggacaattat ttaaagtgac cactttcggc gaatctcacg gtattgcatt aggctgtatc
     1861 gtagatggcg taccgccgaa tatggcttta agcgaagcag atttacagcc ggatttagac
     1921 cgccgtaaac ccggtacttc acgctacacc acgcctcgcc gtgaagatga tgaagtgcaa
     1981 attttatcgg gcgtctttga aggcaaaacg acgggaacaa gcattggttt aatcattaaa
     2041 aacggtgacc aacgctcaca agactatggc gacattgccg aaaaattccg cccggggcac
     2101 gcagattata cctatcagca aaaatacggc attcgagatt accgtggcgg tgggcgttct
     2161 tctgctcggg aaaccgcaat gcgggttgcc gccggggcga ttgccaaaaa atatttgcgt
     2221 gagcaatttg gcattgaagt gcgaggctac ctctcacaaa tcggcaatat caaaattgac
     2281 ccgaaaaccg ttgaaaatat tgaacaaatt gattggcacg cagtcaatag taatcctttc
     2341 ttctgccccg atcccgttgc gattgaaggc tttgatgcct taattcgtga gctgaaaaaa
     2401 gatggcgatt cggtgggggc aaaattaacc gtgattgcag agcgtgtacc tgtagggtta
     2461 ggcgaacctg tgtttgaccg cctagatgcc gaccttgccc acgccttaat gtcgatcaac
     2521 gccgtgaaag cggtggaaat tggcgatggt tttgcagttg tggaacaacg tggtagccag
     2581 caccgagatg agatgacgcc gcaaggcttt ctctctaatc acgccggcgg aattttaggt
     2641 ggtattagct ccggtcagcc gattatcgct catattgcgt taaagccgac ctcaagcatt
     2701 actattcccg gcagaagcgt gaacaaaaac aacgaagccg ttgaagtgat caccaaaggc
     2761 agacacgacc cctgtgtagg tattcgtgct gtgccgattg cagaagcgat gatggcgatt
     2821 gtactactcg accatctgtt aagatttaag gctcaatgcc gttaaacaca taaaattgga
     2881 aaaatgaaat tattaaaaac acttttagtc tcaagccttg cttttacagc taccgcactg
     2941 aatgcaacgc aatgggagaa aatcaaaact ccggttcaag gaaaagcaca atctatcggc
     3001 ggttatagta acggctgtat tattggggca cagccccttg cattaaaagg cgaaggctac
     3061 caagtgatcc gctcggctcg taaccgctat tacggacacc cacaactgct cgattatctc
     3121 acttacttag gcaaaaaaac caaagctgcc ggtgtgccaa ccattttaat cggcgatatg
     3181 ggaatgcctg ccggtgggcg ttttgcctcc ggtcacgcct cccatcaaac cgggctggat
     3241 gctgatattt ggtttaagtt cggaccgatg gacgatgaaa ccgcccgcaa tccagcaggg
     3301 cttgccacca ttatggtaaa ccgtagtacc gagcgggttg atgattcact ctggaatcaa
     3361 caccatacta atttagtaaa acttgctgca accgatcctc gagtggatcg catttttgtc
     3421 aatcctgcca ttaaagtgaa actctgccaa accgcaggca atgatcgtgc ttggttacgc
     3481 aaaattcgcc cgtggtacgg acacgactcc catttccacg tccgtttaac ctgcccggct
     3541 gatgccgcaa gttgtgaaaa tcaagcccct gtgcctgccg gtgacggctg tggtgaagag
     3601 ctttactcgt ggtttgagcc gaaagaagcc tccggcacac caaaagccaa agtgctaccc
     3661 acagcgcctg ttcaatgtca gatgatttta tctgaacaag gcttgaatta attgcaaaat
     3721 aacaaccttt tttgaccgct tgtcagccca caagcggttc attttcttaa ataatttacg
     3781 attatgcgtt tatttgttgc cgaaaaaccc agccttgccc gagcgattgc cgatgtgtta
     3841 ccaaaacccc ataccaaagg agatggcttt atccaatgcg gcgaacacga cactgttact
     3901 tggtgtatcg gtcatttatt ggaacaggct gaacccgatg tctatgatga gcgtttcaaa
     3961 atgtggcgaa tggaacattt gccgattgtg cctgaacaat ggaaattgat tcccaaaaaa
     4021 gagacgctca agcagtttaa agtggtggaa aagctgatta aaaaagccga tgttttagtc
     4081 aatgccggag acccggatcg agaagggcag ctgttggtgg acgaagtttt tggctatttg
     4141 aatttatctg ctgaacgcaa aagccaaatt cagcgttgtt tggtaagcga tctcaacccg
     4201 aatgtggtga gccgagccat tgaaaaattg caaaaaaaca gcgattttat accgcttgca
     4261 acctctgctc tcgcccgagc cagagcggat tggctgtatg gtatcaatat gacccgagcc
     4321 tataccctac aggggcgttg ggcaggctat aaaggcgtgt tatcagtcgg acgtgtgcaa
     4381 acgccggtat tagggctgat tgttcgccga gatctggaga tcgagcattt cgtgccaaag
     4441 gattattttg aggtgttggc acagattgtc gtgccggaaa cccaagagcg atttaaggcg
     4501 cagtggcaac cgagtaaagc ctgtgaagat tatcaagatg aagatgggcg ggtgctgtct
     4561 cgtccattgg cagaaaatgt ggtaaaacgg attaccgacc agccggcaac cgtcaccgaa
     4621 tatcaaaata aaattgaaaa agaaactgca ccgctgcctt attctctttc ggtattacaa
     4681 atggaagccg ccggcagata tgggctatcc gcccaagcgg tgttggatat ttgccaaaag
     4741 ctgtatgaaa cccataaact aattacctac cctcgttctg ataatcggta tttgccgacc
     4801 gaacatttca acgacagatt taaagtgatg gcggcgattg ctcatcattt acctgaatat
     4861 caacaaaaac cggaagtgat tgatgccact attcgcaatc gttgctggaa tgataaagag
     4921 gtggaagccc accacgccat tattcccact gcacggcaag ggacggtaca tttaaccgaa
     4981 aatgaaaaaa gagtttatca actcattgcc cgccaatatc taatgcaatt ctgccctgat
     5041 gcggaatatc gcaaaagtaa aatcgggctt gaaattgcag gtggtagttt tgtcgctcaa
     5101 gcacgcaacc ttgtaattgc cggctggcga gcgttactgg gtaaagaaga tagccttgac
     5161 gatgcagagc cactactacc gatt
//
LOCUS       DANJWC010000159         5135 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.159, whole genome shotgun sequence.
ACCESSION   DANJWC010000159 DANJWC010000000
VERSION     DANJWC010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5135
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.159"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..194)
                     /locus_tag="PWX02_002078"
     CDS             complement(<1..194)
                     /locus_tag="PWX02_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+/Pb2+ permease"
                     /protein_id="HDL1730051.1"
                     /translation="MTALQHDFEQISQHDSLAGVEVQTALKKAIETPNAENLESLSKK
                     LYAFEKEQNPSGYAEKHQDFV"
     gene            complement(198..341)
                     /locus_tag="PWX02_002079"
     CDS             complement(198..341)
                     /locus_tag="PWX02_002079"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730052.1"
                     /translation="MKLKALVLIPLLFSSTGLLAKVNVSPLFVQLSDAMAESKKGEFA
                     KSS"
     gene            complement(352..1548)
                     /gene="efeB"
                     /locus_tag="PWX02_002080"
     CDS             complement(352..1548)
                     /gene="efeB"
                     /locus_tag="PWX02_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HDL1730053.1"
                     /translation="MMAETTSRRDFLKHTALVGAGLLSAPAFALESRQISEKSQNQYD
                     FYGKHQAGIVSPAQKHIYFLVLDLDTTDIAKVKTLFQNWTEYSRNLTLGKNVKPYSAN
                     AYVPPADTGEADSLSAQNLTLTFGVSANFFTKLGIEKHKPKELNDLPHFPRDQLQAEY
                     TGGDICIQACADDPQVAFHAVRNLVRVARGEIKMKWSQMGFNSFANQDTPRNLFGFKD
                     GTANAESLKDQENTVWVQQEGWLKGGSYLAVRRVKMFLETWDRTHLQSQEETFGRHRD
                     SGAGIGHQHEFDKLDLAQKDEKGNAVIPEVSHAHLANKTGLKILRRSFSYSSGIDDKG
                     QFDSGLLFVSFQQSPEQFIKIQNALGNVDKMNEYITHIGSGLFACFAGVKDENDYLGK
                     ALFDLI"
     gene            complement(1559..2371)
                     /locus_tag="PWX02_002081"
     CDS             complement(1559..2371)
                     /locus_tag="PWX02_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EfeM/EfeO family lipoprotein"
                     /protein_id="HDL1730054.1"
                     /translation="MRLTKLAFALSGFFVATQAAALDLTKETEAYKQFVVEQIDQLVT
                     DTEKFVGYLNKGDVQKAKKIYPLARMYFERSEPIAESFGDLDPRIDARLADLAEEGKT
                     EKDWSGFHKIEKVLWQKNTTKGTKATAEQLLKDVKELRAKIPTAEVTPELMITGAVDL
                     LNEVSTTKVTGEEEIFSKTDLYDFKANIEGAEKIYEIFKTQLEQIDAKLSTEIGSRFA
                     AVNALLAKHNKSKTGYDYVPYNKLSKADIKALAEAVNQLGEPLAQLGVLLNK"
     gene            2559..2801
                     /locus_tag="PWX02_002082"
     CDS             2559..2801
                     /locus_tag="PWX02_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDL1730055.1"
                     /translation="MSRTTSVTLAEPLSQFVNQMVESGRYGSTNEVISAALKILEAQE
                     QQLIQLRALIDEGLESGVSEETVQSIISKAKAKRNV"
     gene            3040..5058
                     /gene="uvrB"
                     /locus_tag="PWX02_002083"
     CDS             3040..5058
                     /gene="uvrB"
                     /locus_tag="PWX02_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HDL1730056.1"
                     /translation="MSKQGKPFILHSPFKPSGDQPSAIAKLTEGLNDGLAHQTLLGVT
                     GSGKTFTIANVIAKLNRPAMVLAPNKTLAAQLYAEMKAFFPENAVEYFVSYYDYYQPE
                     AYVPASDTFIEKDASINEQIEQMRLSATKSFLERRDTIVVASVSAIYGLGDVDAYMQM
                     MLHLQVGAMIGQREILSRLAELQYTRNDQAFQRSTFRVRGDVIDIYPAESDEVALRVE
                     LFDDEIENLSLFDPLTGRSLGKVPRYTIYPKTHYVTPRERILEAIEQIKVELADRRTY
                     FIKENKLLEEQRIAQRTQFDIEMMNELGYCSGIENYSRYLSGRKEGEAPPTLFDYMPA
                     DGILVIDESHVTVPQIGGMYRGDRARKETLVQYGFRLPSALDNRPLRFEEFERLSPQT
                     IYVSATPGAYELEKNPDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIHKRVAVDER
                     VLVTTLTKKMAEDLTDYLDEHGVRVRYLHSDIDTVERVEIIHDLRMGMFDVLVGINLL
                     REGLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNLNGKAILYGDRITNSMQKA
                     ITETERRREKQMKYNEEHGITPQALNKKVGELLDIGQSVKPKRGKQAVKKAENSATDY
                     KPKSRKELEKELKTLEQQMRDFAKDLEFEKAAAVRDKLQQLKAYLLEV"
BASE COUNT         1440 a         1092 c         1126 g         1477 t
ORIGIN      
        1 acaaaatcct gatgtttttc agcataaccg ctagggtttt gttctttttc aaacgcatag
       61 agttttttag aaaggctctc taagttttca gcattgggtg tttcaatcgc ttttttcaga
      121 gcggtctgta cttcaactcc ggcgagggaa tcgtgctggc taatctgctc aaaatcgtgc
      181 tgtaaagcgg tcaaatttta gctggatttt gcaaattccc cttttttact ctctgccatt
      241 gcatcggaaa gctgaacaaa taaggggctg acattcactt tggcaagcaa tcctgtactc
      301 gaaaagagta acggaattag cactaatgct tttaatttca tttccttttc cctaaattaa
      361 atcaaacaac gctttgccaa ggtagtcatt ttcatctttc acaccggcaa aacaggcaaa
      421 taaaccactg ccgatatgag tgatgtattc attcatttta tccacattgc cgagagcgtt
      481 ctggattttg ataaattgct ccggactttg ttggaaagag acaaacaaca accctgaatc
      541 aaattgccct ttatcatcaa taccgcttga ataggagaat gaacggcgta ggattttcag
      601 ccccgtttta ttggcaagat gagcgtgtga aacttccgga attactgcat tgcctttttc
      661 gtctttttgg gctaaatcta atttatcaaa ttcgtgctga tgcccgattc cggctccgga
      721 atcacggtga cgaccgaagg tttcttcttg ggattggaga tgggtgcgat cccaagtttc
      781 caagaacatt ttaacacggc gaacggctaa ataagaaccg cctttgagcc agccctcttg
      841 ttgcacccat accgtatttt cctgatcttt taaactttct gcgttggcag tgccgtcttt
      901 aaaaccgaat aagttacgag gtgtgtcttg attggcaaag gagttaaacc ccatttgcga
      961 ccatttcatt ttgatttcac cacgggcaac acgcaccaaa ttacgcacag cgtggaaagc
     1021 cacttgcgga tcgtctgcac aggcttgaat gcaaatatcg ccaccggtgt attctgcttg
     1081 tagttgatca cgagggaaat gcggtaaatc attcagttct ttcggcttat gtttttcaat
     1141 gccgagcttt gtaaagaaat tggcactgac gccaaaagta agagtgagat tctgtgccga
     1201 aaggctatct gcctcaccgg tatccgccgg tggaacataa gcattagcac tataaggttt
     1261 cacatttttg ccaagcgtga ggttacggct atattccgtc cagttttgaa acaatgtttt
     1321 tactttggca atgtcagtgg tgtctaaatc cagcactaaa aagtagatat gtttttgtgc
     1381 agggctaaca ataccagctt gatgtttgcc gtaaaagtcg tattgatttt gagatttttc
     1441 agatatttgt cgtgattcca aagcgaaagc aggagcagac aataagcctg ctcctactaa
     1501 tgcggtatgt tttaaaaaat cacggcgaga agtggtttct gccatcattt ttgctccact
     1561 atttatttaa aagtacgcca agttgagcta acggctcgcc taattggttt acagcctctg
     1621 ctaacgcttt aatatccgct tttgacagtt tgttataagg cacataatca taaccggttt
     1681 tagatttatt gtgttttgct aacagcgcat tgacagccgc aaaacgagag ccgatttctg
     1741 ttgagagttt ggcatcaatt tgctctaatt gcgttttaaa aatctcatag attttttctg
     1801 caccttcaat attggcttta aaatcgtaaa ggtcggtttt agagaaaatt tcttcttcac
     1861 cggttacttt agtagtagaa acttcgttta ataaatctac cgcaccggta atcatcagct
     1921 caggggtaac ttctgcagtg ggaattttcg cacgcagctc ttttacatct tttaataatt
     1981 gttctgccgt tgcttttgtg cctttagtgg tgtttttctg ccacaatact ttctcgattt
     2041 tatggaaacc tgaccaatct ttctcggttt taccttcttc ggctaaatct gccagacggg
     2101 catcaatacg agggtctaaa tcgccaaaac tttctgcaat cggttcagaa cgttcaaaat
     2161 acattcttgc aagcgggtaa attttcttcg ctttttgcac atcgcctttg tttaagtagc
     2221 cgacaaattt ttccgtatcg gtaaccagtt ggtcaatctg ttctaccaca aattgtttat
     2281 aagcctcggt ttccttagtt aagtctaaag ctgccgcttg agtagccaca aaaaagccag
     2341 aaagtgcaaa agcaagtttg gttaaacgca tagaaatttc cttttaataa aaatattgcg
     2401 acataatatc cttattgcga attattcgca aaaaataatg ctgattttgt gatgtaaatc
     2461 acattttgtt ttgcaaatta ttgctaaaaa tagaccgctt gcttatctaa attagagcca
     2521 ttacaatctg ctcaataatc aataagatag gaatagtaat gagtagaaca accagcgtaa
     2581 ctttagctga gccattaagc caatttgtga accaaatggt tgaaagtggg cgttatggtt
     2641 cgaccaacga ggtcatcagt gccgcattaa aaatattgga agcacaagaa cagcaactaa
     2701 ttcagctaag agccttaatt gatgaaggct tagaaagcgg tgtttctgag gaaaccgttc
     2761 aatctattat cagcaaggca aaggcaaaac gtaatgtata aactttctgt tagggcagag
     2821 cgagatttag cgttacttta ttcgcccttt gttacgctgt cattaccacc gctcacattc
     2881 tatttattat aaaccaatcc caaaaggtat ctttattgtt agggtgttag atcaaagaat
     2941 ggatcaatat aagcagttac aataaatttg caaaaatccc gataaaaacg accgcttgct
     3001 tgtgctaaaa taaaccgatt ttttaagaca ggattttcta tgagcaagca aggcaaaccc
     3061 tttatcctcc attctccttt caagccctcc ggcgaccaac caagtgcgat tgccaaattg
     3121 accgaaggct taaatgacgg cttggcacat caaaccttac ttggcgtaac cggctcgggg
     3181 aaaaccttta ccattgctaa tgtgattgcc aagctcaatc gcccggcgat ggtgcttgcc
     3241 ccgaataaaa cccttgccgc ccagctttat gccgagatga aagcgttctt tccggaaaat
     3301 gcggtggagt atttcgtctc ttactatgat tactaccagc ccgaagccta tgtgccggca
     3361 agcgatacct ttatcgagaa agatgcctct atcaatgagc agatcgagca aatgcgactt
     3421 tccgcaacca aatccttttt ggaacgccga gatacgattg tagtggcgtc cgtttctgcc
     3481 atttacggct tgggtgatgt ggacgcttat atgcagatga tgttgcattt gcaagtgggg
     3541 gcgatgattg ggcagcgtga aattctctct cgccttgccg aattgcaata tactcgcaac
     3601 gatcaggcgt tccaacgttc gactttccga gtgcggggcg atgtgattga tatttaccct
     3661 gccgaaagcg atgaagtagc gttaagggta gagctgtttg acgatgaaat cgaaaatctg
     3721 tcgctgtttg atccgcttac agggcgtagc ttgggcaagg tgcctcgcta taccatttac
     3781 ccgaaaaccc actatgtgac tcctcgggaa cggattttgg aggcaatcga gcaaattaag
     3841 gtcgagcttg ccgaccgtcg cacctatttc atcaaagaga ataaattgct ggaagagcag
     3901 cggatcgccc aacgtacgca gtttgatatt gagatgatga acgagctggg ctactgttca
     3961 ggcattgaaa actactcacg ctacctctcg ggcagaaaag agggcgaagc ccctccgacc
     4021 ctgtttgatt atatgccggc ggacggtatt ttggtgattg atgaaagcca cgttactgtg
     4081 ccacaaattg gcggaatgta tcgaggcgac cgagcgagaa aagaaacatt ggtgcaatac
     4141 ggtttccgtc tgccgtctgc actggataac cgcccgttga gatttgaaga gtttgaacgt
     4201 ctttcaccac agacgattta cgtttctgcc acaccggggg cgtatgaatt ggagaaaaat
     4261 cccgatgtgg tggatcaagt agttcgtcca accggcttgc tcgatccgat tatcgaagtt
     4321 cgccccgttg ctacgcaggt ggacgattta ctgtctgaaa ttcacaaacg agtggcggtg
     4381 gacgaacggg tgctggtgac aacactcacc aaaaaaatgg cggaagatct caccgattat
     4441 ttggacgaac atggcgtacg agttcgttat ctacatagcg acattgacac ggtggaacga
     4501 gtcgaaatta tccacgattt acgaatggga atgtttgatg tgctggtggg gatcaactta
     4561 ttgcgtgagg gcttagatat gcccgaagtg tcgctggttg ccattcttga tgcggataaa
     4621 gagggatttt tacgttccga acgctcgtta attcagacca tcggacgtgc cgcccgaaac
     4681 ctcaacggaa aagcgatttt atacggcgac cgcattacca actcaatgca aaaagcaatt
     4741 actgagaccg aacgccgccg tgaaaagcag atgaaataca acgaagaaca cggcatcacg
     4801 ccacaagcgt tgaacaaaaa agtgggtgaa ttgctcgaca tcggacaatc cgtcaagcca
     4861 aaacggggca aacaagcggt caaaaaagcc gaaaattctg caaccgacta caagccgaaa
     4921 tcccgtaaag aactggaaaa agagcttaaa acgctggaac aacaaatgcg tgattttgcg
     4981 aaagatctgg aatttgaaaa agcggctgcg gtaagggata agttgcagca gttaaaggcg
     5041 tatttgttgg aggtttaaat atgatttctc gttcattatt ttcaaaatta atcttatttg
     5101 ccaaaattgt tattttctct caacttgttt ttatg
//
LOCUS       DANJWC010000160         5104 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.160, whole genome shotgun sequence.
ACCESSION   DANJWC010000160 DANJWC010000000
VERSION     DANJWC010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.160"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..648
                     /locus_tag="PWX02_002084"
     CDS             <1..648
                     /locus_tag="PWX02_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00153 family protein"
                     /protein_id="HDL1730057.1"
                     /translation="QSPLKPLQKHSNKVTECSELLESFFEATFEHDWEKAAEVRGKIV
                     DLERRADALKREIRLKLPRGLFMPVERGDLLELVTQLDKLANYSRDISGRIIGRKLII
                     PTEMQPLFKKFLSRSIDASRQVRKVLDEMDQLLETGFRGRELDFVNKMILELDQIEDD
                     TDQYQITLRHTLLELEKNLNPIDVMFLYKCIERISVLADQAQRIGSRIELMLAKS"
     gene            660..1928
                     /locus_tag="PWX02_002085"
     CDS             660..1928
                     /locus_tag="PWX02_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HDL1730058.1"
                     /translation="MELLNQYGSILVIITAIVGFMMAFGIGANDVSNAMGTSVGSGTI
                     TIKQAILIAMVFEFAGAYLAGGEVSDTIKSGIIDPAQFANSPDILVLGMMASLCAAGM
                     WLIIATKMGWPVSATHTIIGAVIGFALITIGSDAIQWDALGGIVGSWFVTPILAGIVA
                     YLIFINSQKLIFNRSNPFKQAKKYGPMYMGITIFILVLVTVSKGLKHVGLHLDTAETL
                     GISFALALLAFIIGYFYFRSKSFAMKARAEGKGFAGVEKIFSILMLITACAMAFAHGS
                     NDVANAIGPLAAVESIVRSGGMIEGKTTLAPWILPLGALGMMIGLAIMGKNVMATVGT
                     GITDLTPSRGFAAQFACAVTVVIASGTGLPISTTQTLVGAVLGVGFARGIAALNLGVI
                     RNIVISWVITLPVGAVLSIIIYYILSAIFY"
     gene            1983..2594
                     /locus_tag="PWX02_002086"
     CDS             1983..2594
                     /locus_tag="PWX02_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDL1730059.1"
                     /translation="MKKLSSALLSCFLLGLSSPALTADTYITENLSTFMRKGAGDQFR
                     ISGAIQAGEKVTVLDRKERYSLIRDSRNREGWVLNSDLSDTASPKELIPQLKQQVQDL
                     TNRLSKIDTEWQQRTAEMQRRTQDSVQKSSDLVEENAQLKRELEILKNKNRDLETMHD
                     SEKREIVIQWFIYGGAVLGAGLLLGLLVPFLIPRRRRNNSGWA"
     gene            complement(2648..3946)
                     /gene="aroA"
                     /locus_tag="PWX02_002087"
     CDS             complement(2648..3946)
                     /gene="aroA"
                     /locus_tag="PWX02_002087"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDL1730060.1"
                     /translation="MEKLTLTPISRVEGEINLPGSKSLSNRALLLAALATGTTQVTNL
                     LDSDDIRHMLNALKALGVKYELSDDKTVCVVEGIGGAFKVQNGLSLFLGNAGTAMRPL
                     AAALCLKGAETAQIILTGEPRMKERPIKHLVDALRQVGAEVQYLENEGYPPLAISNSG
                     LQGGKVQIDGSISSQFLTALLMSAPLAESDMEIEIIGDLVSKPYIDITLSMMNDFGIT
                     VENRDYKTFLVKGKQGYVAPQGNYLVEGDASSASYFLASGAIKGKVKVTGIGKKSIQG
                     DRLFADVLEKMGAKITWGEDFIQAEQSPLKGVDMDMNHIPDAAMTIATTALFAEGETV
                     IRNIYNWRVKETDRLTAMATELRKVGAEVEEGEDFIRIQPLALENFQHAEIETYNDHR
                     MAMCFSLIALSNTEVTILDPNCTAKTFPTYFRELEKLSVR"
     gene            complement(3992..5086)
                     /locus_tag="PWX02_002088"
     CDS             complement(3992..5086)
                     /locus_tag="PWX02_002088"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDL1730061.1"
                     /translation="MQFINIANEGVKSLSPYQAGKPIEELERELGIQNIIKLASNENP
                     FGFPESAKKAILNQLDDLTRYPDSNGFELKTTIAQKFGVQANQITLGNGSNDLLELFA
                     HTFAGEQDEIIYSQYAFIVYPLVTKAINAVAREILAKNWGHNLDAFLAAITEKTKLIF
                     IANPNNPTGNFLTQAEIEGFLAKVPANVIVVLDEAYTEFTAPQERVDSFGLLAKYPNL
                     IVSRSLSKAYGLAGLRIGYAVSNPEIADLLNRVRQPFNCNSLALASAIAVMNDDEFVK
                     KVAENNRLEMARYEAFCQANGLEYIPSKGNFITIDFKRPAAPIYEALLREGVIVRSIA
                     GYGMPNHLRISIGLPQENERFFTALLKVLA"
BASE COUNT         1449 a         1049 c         1040 g         1566 t
ORIGIN      
        1 cagtccccac ttaaaccgtt acaaaaacac tcaaataaag tgactgaatg tagcgagtta
       61 ttagaatctt tttttgaagc gacttttgaa catgattggg aaaaagcagc tgaagtgcgc
      121 ggtaaaattg ttgatttaga acgccgcgca gatgccctta aacgtgaaat ccgcttaaaa
      181 ctgcctcgtg gtttatttat gccagttgaa cgcggtgatt tattagaact agtcacacaa
      241 ttagataagt tagcaaacta ttcaagagat atttccggtc gcattattgg tcgtaaatta
      301 atcattccta ccgaaatgca accacttttc aaaaaattcc tttctcgtag tattgatgct
      361 agccgtcagg tacgcaaagt tttggatgaa atggatcagt tattagaaac tggttttaga
      421 gggcgagagc ttgatttcgt caataaaatg attcttgaac ttgaccaaat tgaagatgat
      481 accgaccaat atcaaattac attacgtcat acacttcttg agttagaaaa aaacctgaac
      541 cctattgatg tgatgttttt atacaaatgt attgaaagaa tcagcgttct agctgaccaa
      601 gcccagcgaa ttggttctcg tattgaactt atgctggcga agtcatagga gaaaattaaa
      661 tggaattact caatcaatat ggctcaatac tggtgattat tactgctatt gtagggttta
      721 tgatggcttt cggtattggt gctaatgatg tttcaaatgc catgggaaca tccgtcggct
      781 ctggaacaat tacgattaaa caagctattt taattgctat ggtatttgaa tttgctggtg
      841 cttatctggc aggcggtgaa gtctcagata caattaaaag tggcattatt gatccggcac
      901 aattcgccaa tagccctgat atcctggtac ttggaatgat ggcatctctc tgtgctgccg
      961 gaatgtggct aattattgca actaaaatgg gttggccggt ttcagcaact cacacaatca
     1021 ttggtgccgt tattggtttt gcattaatca ctattggttc agatgcaatt caatgggatg
     1081 cattaggtgg aattgttggg agttggtttg tgacccctat tcttgcgggg atcgtagcat
     1141 atttgatctt tattaactca caaaaactga tctttaaccg ctcaaaccca ttcaaacaag
     1201 cgaaaaagta tggcccgatg tacatgggaa ttactatttt tattttagtt cttgtaaccg
     1261 tgtcaaaagg cttaaagcac gtaggattac atctagatac agctgaaaca ttgggaattt
     1321 cattcgcgct tgccttactt gcttttatca ttggatattt ttatttccgc agtaaaagct
     1381 ttgcaatgaa agctcgtgca gaagggaaag gcttcgctgg cgttgagaaa atttttagta
     1441 ttttaatgct tattacggcc tgtgctatgg cttttgctca tggctctaat gatgttgcta
     1501 atgctattgg gcctctagct gctgttgaat ctattgtaag aagtggtgga atgattgaag
     1561 gtaaaacgac tttagcccct tggattctcc cactaggcgc cttaggtatg atgattggtt
     1621 tagctattat gggcaagaat gtaatggcta cagtaggtac tggtattacc gaccttactc
     1681 caagccgtgg ctttgctgct caatttgcct gcgccgtcac ggttgtcatt gcatcaggta
     1741 ctggtttacc aatatcaaca acccaaacgc ttgtaggagc agtattaggt gtgggattcg
     1801 cccgtggtat tgctgcactt aacttaggcg ttattcgtaa tattgttata tcatgggtaa
     1861 ttaccttgcc tgtaggtgct gtgctttcaa ttattatcta ttatatttta agcgctattt
     1921 tctattaatt tttcaaaaag aatacaggca actgtattct tcctagcttt agaggaatat
     1981 atatgaagaa attatcatca gctcttctgt cttgtttttt actcggccta tctagccctg
     2041 ctttaacggc agacacttac attactgaaa atctgagtac atttatgcgc aaaggtgctg
     2101 gtgatcaatt ccgcatttca ggcgctattc aagcaggtga aaaagtcaca gtattagatc
     2161 gtaaagagcg ctacagtctg attcgagata gtcgtaaccg tgaaggttgg gtacttaatt
     2221 cagacttaag cgatactgct agtccaaaag agctgattcc tcaattaaaa caacaggttc
     2281 aagatttaac caatcgcctg agcaaaatcg ataccgaatg gcaacaacgt accgcagaaa
     2341 tgcaacgtag aacgcaagat tcagtgcaga aaagcagtga tttggttgag gaaaatgcac
     2401 aactaaaacg tgaactggaa atcctgaaaa acaaaaatcg tgatttagaa acaatgcacg
     2461 attccgagaa acgtgaaatc gtgattcagt ggttcatcta tggtggtgct gtgttaggcg
     2521 caggtttact acttgggtta ctcgtgcctt tcctgatccc tcgccgcaga cgtaacaaca
     2581 gcggttgggc ttagtctgcc acaataaaac gtaaaaagga ttcagttttc tgaatccttt
     2641 tttactttta tctgaccgat aatttttcca actccctaaa gtaagtcggg aacgttttag
     2701 cggtacaatt tggatctaag atcgtcactt ctgtattcga taacgcaatt aatgaaaaac
     2761 acattgccat acggtgatcg ttataggttt caatttcagc gtgctggaag ttttctaacg
     2821 caagcggttg aatccgaata aaatcttccc cttcttctac ctctgccccg actttacgca
     2881 attcggttgc cattgctgtc aagcggtcgg tttcttttac ccgccagtta taaatattgc
     2941 ggataactgt ttctccttcg gcaaataaag cggttgttgc aatcgtcatt gccgcatcag
     3001 gaatatgatt catatccata tctacgcctt ttagcgggga ttgctcggct tgaataaaat
     3061 cctctcccca agtgattttt gcccccattt tttccaacac atcggcaaac aagcggtcgc
     3121 cttggatcga ttttttacca atgcccgtta cttttacctt gcctttaatc gcaccggagg
     3181 ctaagaaata agaggcagaa gaggcatctc cctccaccaa ataattacct tgtggagcaa
     3241 catagccttg tttaccttta actaaaaagg ttttgtaatc tcgattttca accgtaatac
     3301 caaaatcgtt catcatcgaa agggtaatat caatataagg ttttgatacc agatcaccga
     3361 taatctcaat ttccatatcg ctttccgcta atggggcaga catcagcaat gcggttagaa
     3421 attggctgga aatcgagccg tcaatttgca cttttccacc ttgcaaaccg ctattgctaa
     3481 ttgctaacgg cggatagcct tcattttcta aatactgtac ctctgcccct acttggcgta
     3541 aagcatcgac taaatgttta atcgggcgtt ctttcattct tggctcaccg gtaagaatga
     3601 tttgagctgt ttccgcccct tttaaacaca atgctgctgc aagtggtcgc attgccgtgc
     3661 ctgcattgcc gagaaacagt gataagccgt tttgaacctt aaaagctcca ccaatccctt
     3721 caactacaca gacggtttta tcgtccgata gctcatattt cacgcctaac gcttttaagg
     3781 cattgagcat atgtcgaata tcatcactat ctaataaatt ggtcacttga gtcgtaccgg
     3841 tggctaaggc ggctaataat aaggctcggt tagacaggct tttagaacca ggtaaattga
     3901 tctcgccttc tactcgggaa atcggggtta aagttagttt ttccattttt ctctttaaat
     3961 ttgtaaaatt ttcataaaag gtaaccgctt gttaagctaa cactttcaat aaggcagtaa
     4021 aaaatctctc gttttcttgc ggtaaaccga tactaatgcg taaatgattc ggcattccat
     4081 aacctgctat cgagcgaaca atcacgcctt cacgcagtaa tgcctcataa atcggagcgg
     4141 caggacgttt gaaatcaata gtaataaaat tgcccttaga agggatatat tccaagccgt
     4201 ttgcttggca gaaagcctca tagcgagcca tttccaagcg gttattttct gccacttttt
     4261 tgacaaattc atcatcattc attaccgcaa tcgctgaagc taaggcaaga ctattgcagt
     4321 taaacggctg gcgaacacga tttaataaat ccgcaatttc agggtttgaa accgcataac
     4381 caatccgaag cccggctaag ccgtaagcct tagagagtga acgtgacaca attaaattcg
     4441 ggtattttgc caataagcca aacgaatcca cccgttcttg aggcgcagta aactcggtat
     4501 aagcttcgtc cagcaccacg attacatttg ccggcacttt agctaaaaag ccctcaattt
     4561 ccgcttgggt taaaaaatta ccggtcggat tattcgggtt ggcaataaaa attaatttag
     4621 ttttttcggt aattgcagcc aaaaaggcat ctaaattgtg tccccaattt ttagccagaa
     4681 tttcacgggc aacggcatta atcgctttcg tcaccaacgg atacacaata aacgcatact
     4741 gagaatagat aatttcatct tgctcaccgg cgaaggtgtg ggcaaacagc tctaataaat
     4801 cgtttgagcc attgcctaag gtgatttgat tggcttgtac gccaaatttt tgggcaatag
     4861 tggtttttaa ttcaaagccg tttgaatccg gatagcgggt taaatcgtca agctgattca
     4921 gaatcgcctt ttttgcactt tcaggaaaac caaacgggtt ttcgttcgag gctaatttaa
     4981 tgatattttg aatccctaat tcacgctcta gctcctcaat cggctttcct gcttggtaag
     5041 gcgaaaggga tttcacgccc tcatttgcga tgttgataaa ttgcataata acctctgatt
     5101 attc
//
LOCUS       DANJWC010000161         5049 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.161, whole genome shotgun sequence.
ACCESSION   DANJWC010000161 DANJWC010000000
VERSION     DANJWC010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.161"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            49..345
                     /locus_tag="PWX02_002089"
     CDS             49..345
                     /locus_tag="PWX02_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HDL1730062.1"
                     /translation="MYIDLQYAVPLLFEYDPNKSQINLAKYGIDFEQAKLLWEDERKV
                     VLEACIEPELRYFLIGKIYDKYWTAFFTPRNDVIRLISVRRSRKKEISYYEKYH"
     gene            329..571
                     /locus_tag="PWX02_002090"
     CDS             329..571
                     /locus_tag="PWX02_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HDL1730063.1"
                     /translation="MRNTISAEELERIFDEGKEDILQYCDLSTAHRPGQMKRVNVDFP
                     NWMVEALDKQARHLGVPRQALSKMWIAEKLPVQSPR"
     gene            583..1251
                     /gene="rluA"
                     /locus_tag="PWX02_002091"
     CDS             583..1251
                     /gene="rluA"
                     /locus_tag="PWX02_002091"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HDL1730064.1"
                     /translation="MALIEYNPPLEPFLDEVYRDNHILVINKPSGLLSVPGNRPEYYD
                     SAMTRVQQKYGFTEPAHRLDMATSGILLFALSKAAEKELKRQFREREPKKHYIALLWG
                     KLGEKVGDKGEMNFPLICDWENRPRQKICYERGKKAITHYEVLAHYPNNIARVKLTPI
                     TGRSHQLRVHSLALGHPIIGDKFYANPLAKSLSPRLCLHAESLTITHPITRELMTFTA
                     EPTF"
     gene            complement(1229..1804)
                     /gene="ampD"
                     /locus_tag="PWX02_002092"
     CDS             complement(1229..1804)
                     /gene="ampD"
                     /locus_tag="PWX02_002092"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HDL1730065.1"
                     /translation="MPNLKIEQGCLTGQKQVPSPHFSQRPDQEDISLLVIHYISLPPE
                     EFGGNFIDLFFQGKLEPTAHPYFEEIKDLRVSAHCLINREGEITQYVNFNDMAWHAGV
                     SCFEGREKCNEYSIGIELEGSNNQPFTQAQYQSLANLTKAIQQSYPQITDERITGHCH
                     IAPERKIDPGQYFDWAYYKHLLNEAKKSAQQ"
     gene            1864..2706
                     /locus_tag="PWX02_002093"
     CDS             1864..2706
                     /locus_tag="PWX02_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HDL1730066.1"
                     /translation="MLSYRHSFHAGNHADVLKHIVQLLILRALKEKEKGFLYLDTHSG
                     VGRYSLLSFESEKTGEYLEGVARLWERDDLPEEVAFYIDELKKLNRGGKLKYYAGSPL
                     LAVQQLREQDRAILTELHPNDFPLLRQEFAKAKNVQTKRENGFQQLKSAQPPKERRGF
                     VLIDPPYELKEDYELVVKAIEEGYKRFATGVYAIWYPVVLRQHTKRIVRGLMDTGIRK
                     ILQIELAVRPDSDQRGMTASGMIVINPPWQLETQMKKILPYLTEVLVPEGTGSWKVEW
                     ITPE"
     gene            2829..3470
                     /gene="pyrE"
                     /locus_tag="PWX02_002094"
     CDS             2829..3470
                     /gene="pyrE"
                     /locus_tag="PWX02_002094"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDL1730067.1"
                     /translation="MQQYKHDFIEFALSRNVLKFGEFTLKSGRISPYFFNAGLFNTGR
                     DLAKLGEYYAKAIQASGIEYDVLFGPAYKGIPIATTVAVALANLFDVDKPCCFNRKEA
                     KDHGEGGNLIGSPLTGKILLVDDVITAGTAIRESMEIINANNAQLAGVLIALNRKEKG
                     KGELSAIQEVERDYDCQVFSIVDFDDLVAFIETSPQYAPYLPKMREYREKYGV"
     gene            3644..4721
                     /locus_tag="PWX02_002095"
                     /pseudo
     CDS             3644..4721
                     /locus_tag="PWX02_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607068.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
     gene            complement(4782..>5049)
                     /locus_tag="PWX02_002096"
     CDS             complement(4782..>5049)
                     /locus_tag="PWX02_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HDL1730068.1"
                     /translation="SGLFSFELCEKLSNDKLANFLEHFELFSMAYSWGGFESLILANQ
                     PEEIAKIRPSIERKLTGTLIRIHVGLEAVEDLIADLEQGFNRIR"
BASE COUNT         1522 a          975 c         1146 g         1406 t
ORIGIN      
        1 ttatttatgc tattcctcat attgtaaaag gtataaaaaa ggaatattat gtatatagat
       61 ttgcaatatg ctgtgccgtt attgtttgaa tacgacccga acaaaagcca aattaattta
      121 gccaaatatg gtattgattt tgaacaggca aagttgcttt gggaagatga acgaaaagtt
      181 gtactggagg cttgcattga acccgaatta cgctatttcc tgattggcaa gatttatgat
      241 aaatattgga cagcgttttt tacaccaaga aatgatgtta ttcgtttaat ttcagttaga
      301 cgttcccgta aaaaagagat ctcatactat gagaaatacc attagtgccg aagaattaga
      361 acgtatcttc gatgaaggca aagaagatat tttacaatac tgcgatcttt ctaccgctca
      421 tcgcccgggg caaatgaaaa gggtgaatgt tgatttccca aattggatgg tagaagcgtt
      481 agataagcaa gccagacatt taggtgtgcc tcgccaagcc ttgagtaaaa tgtggattgc
      541 ggaaaaatta ccggttcaat cacctaggta agcgagtggc aaatggcatt aattgaatat
      601 aaccctccat tagaaccatt tttagatgag gtttatcggg ataaccatat tttagtaatt
      661 aataagccta gtggtttatt atcggtgccg ggcaatcgtc ccgaatatta cgacagtgca
      721 atgactcgag tacagcaaaa atatggcttt accgaaccgg ctcatcgttt ggatatggca
      781 acaagcggta ttcttttgtt cgccctaagc aaagcggcgg aaaaagaact aaaacgccaa
      841 tttcgggaac gtgaaccgaa aaaacactat attgcgttac tttggggcaa attaggtgaa
      901 aaagttgggg ataagggcga aatgaatttt ccgctgattt gcgattggga aaatcgcccc
      961 cgccagaaaa tttgctatga acgaggcaaa aaagccatta cccattatga agtgttagct
     1021 cattatccga ataatatagc gagagtaaaa ctcacgccta tcacggggcg ttcgcatcag
     1081 ctacgagtac atagccttgc gttaggtcac ccgattattg gcgataagtt ttatgctaat
     1141 ccgctggcaa aaagtttatc gccccgttta tgtttgcacg cggagtcact aacaattact
     1201 cacccgatta caagggagtt gatgacgttt actgctgagc cgacttttta gcctcgttca
     1261 gtaggtgttt gtaatatgcc caatcaaaat attgccccgg gtcgattttc cgttccgggg
     1321 caatatggca atgcccggtg atgcgttcat cggtaatttg tggatagctt tgctgaatgg
     1381 ctttggtcaa atttgccaaa gattggtatt gagcttgagt aaacggctga ttattactgc
     1441 cttccagttc aatgccaatc gaatattcat tgcatttctc ccgcccttca aaacaagaga
     1501 cgccggcgtg ccacgccata tcgttaaaat tcacatattg cgtgatttcc ccctccctat
     1561 taatcaaaca atgggcggaa acccgtagat ctttgatttc ttcaaaataa gggtgagcgg
     1621 ttggttcgag tttaccttgg aaaaacagat cgataaaatt tccaccaaat tcttccggcg
     1681 gtaagctgat gtagtgaata accaacaagg aaatatcctc ttgatcagga cgttggctaa
     1741 aatgcggaga cggcacttgt ttttgaccgg ttaaacagcc ctgttcgatt tttaaatttg
     1801 gcatagtcta aatagggtga atacgttaaa atatggctca ttttactaga aaaagagaag
     1861 aatatgttaa gttatcgcca cagtttccac gcaggcaacc acgctgatgt gctaaaacac
     1921 attgtccagc ttttgatctt gcgagcatta aaagaaaaag aaaaaggttt tttatatctt
     1981 gatactcact ccggtgtggg gcgttatagt ctgttaagtt ttgaatcgga aaaaacaggc
     2041 gaatatttag agggtgttgc ccgtttatgg gagcgtgatg atttaccgga agaagtggca
     2101 ttctatattg atgagctgaa aaaattaaac cgtggcggca agttaaagta ttacgcaggc
     2161 tccccattat tggcggtaca gcaactgcgt gagcaagacc gagccattct taccgaactt
     2221 caccccaatg attttccgct gttacgacaa gaatttgcca aagcgaaaaa tgtgcaaacc
     2281 aagcgagaaa atggctttca acaattaaaa tccgcccagc cgcctaaaga gcgtcgaggt
     2341 tttgtgttga ttgatccacc ttatgaatta aaagaagatt atgaattggt ggttaaagcg
     2401 atagaagagg gctacaaacg gtttgccact ggtgtgtatg ccatttggta tccggtggtg
     2461 cttcgccaac ataccaaacg tatagtgcga ggtttaatgg ataccggcat tcgcaaaatt
     2521 ctacaaattg agcttgccgt tcgtcctgat agcgaccaac gtggaatgac ggcaagtggt
     2581 atgattgtga tcaacccacc gtggcagctt gaaacccaga tgaaaaaaat cctgccatat
     2641 ttaaccgagg tgcttgtgcc ggaaggaaca ggttcttgga aggtggagtg gattacgcct
     2701 gagtaaatta aatacggata gcacaagcgt ggacgcttgc gccatcataa attctgattt
     2761 gcaatatttt ttagaaaaat aaccgcttgt cttatcaaga agtgggggaa atataaaagg
     2821 aaaaaacgat gcaacaatac aaacacgact ttattgaatt tgccttaagt cgcaatgtgc
     2881 tgaaatttgg cgaatttacc ttaaaatcag gcagaatcag cccctatttt ttcaatgctg
     2941 ggctttttaa caccgggcgc gatttagcca aattaggcga atattacgcc aaagccattc
     3001 aagccagcgg cattgagtat gatgtgttat tcgggccggc ttataaaggc attccgattg
     3061 caaccacggt tgccgtggca ctagccaacc tgttcgatgt ggataaacct tgttgcttta
     3121 accgcaaaga ggcaaaagat cacggtgaag gcggtaattt aatcggttct cccttaacgg
     3181 gcaagatttt gctggtggac gatgtgatta ccgcaggcac ggcaattcgt gagtcgatgg
     3241 aaattatcaa tgccaataat gcccagcttg ccggtgtgct gattgcgtta aaccgtaaag
     3301 agaaaggcaa gggcgagttg tctgcgattc aagaagtcga acgtgattac gattgccaag
     3361 ttttctcgat tgtggatttt gatgatttgg tagcatttat tgaaacgtcg ccacaatacg
     3421 ctccttattt gccgaaaatg cgtgaatatc gtgaaaaata cggtgtataa cttttgcaaa
     3481 aaaacagcaa aaactcaccg cttgtcactc tctaatctca ccattatgaa tcactttcat
     3541 cttttggtga gattttttca attaaaaagc cgataaattg attgtgagct ttagctaatt
     3601 ggcttaggat aaactcctgt aatacaggag ttggagaact aaaatggcac atcaaaaatt
     3661 accttttcac gcagacaccg ttggctcata tttgcgttcc gatgcttgga aaaaagctca
     3721 cgcagactat aaagcaggca acatttccct cgaacaacgt gatgaaattg tagaagcgga
     3781 agtgaaaaaa ttagtacaag ctcaattaga tgcgggcatt caagttgtta cagacggcga
     3841 atatcaccgc tcttggtggc atattgattt cttggaaaat ctgaacggca ttgaaggcta
     3901 tgtgccggaa aaagcctatg cgtttaaagg ggttttataa tacacgttgt tgcggtaaag
     3961 tgtcttggaa tccgaatcac ccttttattg agcatttcaa aaaattcaat gcgattgtag
     4021 gtggtcgtgc ggtggtgaaa tttaccattc caagtccaaa ccaattaatg tacccgattc
     4081 agtgggatca cggcatttac ccaaaccgtg aggaatttgc caaagatgtg caacaggctt
     4141 ataaagaggc gattaaagcg ttctatgatg ccggctgtcg ttatttgcaa attgatgatg
     4201 tctattgggg gtcgttgtgt aataacgttg gtcaaccgac atttgaagcc gataaagctc
     4261 aagcggtggc gaatattcaa gccattcttg ccgataagcc ggaagattta accattacca
     4321 cccacgtttg ccgaggcaac tataaatctt cttatttatt gacaggggct tatgatccga
     4381 ttgcagacgg cttatttgct caaaccaact atgacggcta tttcttagaa tatgatgatg
     4441 agcattcagg cggttttgag ccgttaaaac attttgccaa caacaaaggg cgtgtggtgt
     4501 tgggcttaat tagctcaaaa ttccctgaat tggaagataa agaagcggtt aaagcccgta
     4561 ttcaagaggc ggcaaaatat gtgccgttag agcagctttg tctcagtccg caatgtggtt
     4621 ttgcctcaac cgaagagggc aatataatgt cagaagcaca acagtgggcg aaagtccgct
     4681 ttgtggaaga aattgccaaa gaagtttggg gcgaagatta atgtgagaaa atgcccgcaa
     4741 caagcgggca ttttttgtta attttttgca attcaagcca tttagcgaat acggttaaaa
     4801 ccttgttcta aatcggcaat taaatcttcc accgcttcca agccaacgtg aatgcgaatt
     4861 aatgtacccg ttagttttcg ctcaatagac ggacggattt tggcaatttc ttccggctgg
     4921 ttggcaagaa tcagcgattc aaacccaccc caagaatacg ccattgaaaa cagttcaaaa
     4981 tgctctagga agttggctaa tttgtcattc gagagctttt cgcaaagttc aaatgaaaat
     5041 agaccgctt
//
LOCUS       DANJWC010000162         4988 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.162, whole genome shotgun sequence.
ACCESSION   DANJWC010000162 DANJWC010000000
VERSION     DANJWC010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.162"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(60..136)
                     /locus_tag="PWX02_002097"
     tRNA            complement(60..136)
                     /locus_tag="PWX02_002097"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(100..102),aa:Val,seq:gac)
     gene            complement(200..1012)
                     /gene="apaH"
                     /locus_tag="PWX02_002098"
     CDS             complement(200..1012)
                     /gene="apaH"
                     /locus_tag="PWX02_002098"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HDL1730069.1"
                     /translation="MATYIVGDLHGCFNEFQLLLEKVNYNPKQDELFLTGDLVARGED
                     SLACLRFVKDSANNAKTVLGNHDLHLLATMLGIKRVKPNDKIDAIFEAKDRLDLQNWL
                     RNQPLVIHHPKHQFLLTHAGISPEWDLATTLACAKEAEKVLQSDNYADYIAQMYENKP
                     ERWSEDLQSVERWRYIINVFARMRFCYTDKRLDFDCKLPLEQAPQELLAWFDLDNPLF
                     TQKNIIFGHWASLMGKCTRPNIYALDTGCAWGSHLTMLRWEDKQIFTQKRLK"
     gene            complement(1104..3080)
                     /locus_tag="PWX02_002099"
     CDS             complement(1104..3080)
                     /locus_tag="PWX02_002099"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HDL1730070.1"
                     /translation="MFQNNPLLAQLKQQIEASKEYVAGVVKASDKAFGFLECDKKSYF
                     IPPAEMKKVMHGDSVKAVVKRDGDKEQVEIDSLLEPMLDRFIAQVRLNKEGKLQLAVD
                     HPSIKQTIPANTHKKVTEKLENGDWVVAQLKTHPLRDDRFFFAQVTQFICKESDNFAP
                     WWVTLARHEQPREPVANEKNYELHDELAREDLSHLYFTTIDSPSTQDMDDALFIEPIK
                     QNGKRTGWRLVVAIADPTAYIPENSHIEKAARQRCFTNYLPGFNIPMLPRELSDDLCS
                     LVPNEKRPALVGFIETDLNGNLIKETTFTSAWVESKDRLAYDDVSDYLEGVENAWQPQ
                     SEETKQQIEWLHQFTQARIQWRAENALLFKEQGDYTFELNEDGSVKDIHVEYRRIANQ
                     MIEESMILANICCAKFLSQHAKTGVFNTHSGFDPKNYELVKSFLLQTLASDENRDELA
                     ERYSAERLATLEGYCQMQRDIQDYPEKFLELRLRRYLTFAEFKAEVAPHFGLGITDYA
                     TWTSPIRKYGDMVNHRLIKQVLLGKQSQAVEESVLSRLQEARRQNRLVERDIADWLYA
                     RYLAPMVEQAVEFECEIADVSRGGLRAKVIKNGAQIFVPFSSLHDNKEEMEYRQEELA
                     LYIKGEKAYQIGQAVKVKLTEVRLETRSIVGNII"
     gene            3240..3518
                     /locus_tag="PWX02_002100"
     CDS             3240..3518
                     /locus_tag="PWX02_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bor family protein"
                     /protein_id="HDL1730071.1"
                     /translation="MKKLLAIATVFSLAACSTQTLELSPAKEAQYDRVQHFFVSGFAQ
                     KQEIDAAKICNGADKVGKVETETTFFNGLLGTITYGIYSPRQIRVYCN"
     gene            3543..3839
                     /locus_tag="PWX02_002101"
     CDS             3543..3839
                     /locus_tag="PWX02_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bor family protein"
                     /protein_id="HDL1730072.1"
                     /translation="MKKLLAMSVLTALLTTACTTQTAHIGGKVTDNIKPTKSVTQSFF
                     IGGIGQEETLNVVEVCGSKDKVQQVETVLSGGDILLGLVTIGIYTPRTANVYCK"
     gene            complement(3892..4683)
                     /locus_tag="PWX02_002102"
     CDS             complement(3892..4683)
                     /locus_tag="PWX02_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL1730073.1"
                     /translation="MGILTGKRILVTGLASNRSIAYGIANAMKQQGAELAFTYLNDKL
                     KPRVEEFAKEFGSDIVLPLDVATDESITECFTELSKHWEKFDGFIHAIAFAPGDQLDG
                     DYVNAATREGYRIAHDISAYSFVAMAQAARPFLNENATLLTLSYLGAERAIPNYNVMC
                     LAKASLEAATRVMAADLGKEGIRVNAISAGPIRTLAASGIKNFKKMLSAFEKTAALRR
                     TVTIDDVGNSAAFLCSDLASGVTGEVLHVDAGFSVMAMGELGDEE"
     gene            complement(4827..>4988)
                     /locus_tag="PWX02_002103"
     CDS             complement(4827..>4988)
                     /locus_tag="PWX02_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1730074.1"
                     /translation="LTIETISRLLGRFQKSGMISVQGKYIIINRMEELAKLAGAIKPQ
                     QNIIAQTTA"
BASE COUNT         1418 a          977 c         1051 g         1542 t
ORIGIN      
        1 attgattata agtatggtaa gattaaagcc tgaatcggga tattcaggct taaaaataat
       61 ggtgcgacta gctggactcg aaccagtgac ccccaccatg tcaaggtggt gctctaacca
      121 actgagctat agtcgcgtta acggtgtgca ttctattgga aaaaggaact tagtgcaata
      181 tgcttttggt cgaatgcttt tattttagac gcttttgggt aaaaatttgt ttatcttccc
      241 aacggagcat ggttaaatgg cttccccaag cacagccggt atcaagtgca tagatgtttg
      301 gacgagtgca tttgcccatt aggcttgccc agtgaccaaa gataatgttt ttctgtgtaa
      361 ataacggatt atctaagtca aaccaagcca gcagttcttg aggggcttgt tcaagcggta
      421 atttacagtc aaaatctaac cgcttgtcgg tgtagcaaaa ccgcatacgg gcaaacacat
      481 tgatgatata acgccaacgt tcaacacttt gcaaatcttc actccagcgt tcgggcttat
      541 tctcatacat ttgggcgatg tagtccgcat aattgtcact ttgtagcacc ttttctgcct
      601 ctttggcaca agctagggta gttgctaaat cccactcagg gctgattcct gcgtgagtca
      661 gcaaaaattg atgtttgggg tgatgaatta caagcggttg atttctgagc caattttgca
      721 aatcgagccg atctttagcc tcaaaaatag catcaatctt atcatttggt tttacccgtt
      781 tgatgccaag cattgtggca agcaagtgca aatcgtggtt gcctagcacg gttttggcat
      841 tgtttgcaga gtctttcaca aaacgtaaac aggctaaaga gtcctcacca cgggcgacta
      901 aatcccctgt taaaaagagt tcgtcttgct tggggttata attgactttt tctaataaaa
      961 gctgaaattc gttaaagcaa ccgtgtaggt cgcctacaat ataagttgcc attgtttctc
     1021 ctttgttccc ctaaagttat aaacaagcgg ttatatttta gagaaaattt gcaaaatctc
     1081 atcaaaatat aaccgcttga aatttaaatg atatttccta cgattgagcg ggtttctaag
     1141 cgtacttcgg tgagtttcac tttcaccgct tgcccgatct gataggcttt ttcaccttta
     1201 atataaagtg caagttcttc ttggcggtat tccatttcct ctttgttgtc gtgcaagctg
     1261 gagaatggca caaaaatttg agcaccgttt tttatgactt tcgcacgcaa gccaccacgg
     1321 gaaacatctg caatttcgca ctcaaattcg accgcttgtt ccaccattgg ggcaaggtaa
     1381 cgagcataaa gccagtcagc aatatcacgt tctactaaac ggttttgacg gcgagcttct
     1441 tgtaaacggc ttaaaactga ttcttccacc gcttggcttt gtttgccaag taacacttgc
     1501 ttgattaaac ggtggtttac catatcgcca tatttacgga tgggtgatgt ccaagtggcg
     1561 taatcggtaa tgcctagccc gaaatgcggg gcaacttcgg ctttaaattc agcgaaggtt
     1621 aaataacgtc gtaaacgcaa ctctaagaat ttctctgggt agtcttgaat atcacgttgc
     1681 atttggcaat agccttctaa ggttgctaaa cgctcggcgg agtagcgttc ggcaagctca
     1741 tcacgatttt catcgctggc taaagtttgg agtaaaaatg attttaccaa ttcataattt
     1801 ttcggatcaa aacctgaatg ggtattaaat acaccggttt tggcatgttg gcttaagaat
     1861 tttgcacaac aaatattggc aagaatcatt gattcttcga tcatttgatt cgcaatacgg
     1921 cgatattcaa cgtgaatatc ttttaccgag ccatcttcat ttaactcaaa ggtataatcg
     1981 ccttgttctt tgaatagcaa cgcattctcg gcacgccatt gaatgcgggc ttgagtaaat
     2041 tgatgtagcc actcaatttg ttgttttgtt tcttcagact gaggttgcca cgcattttcc
     2101 acgccttcga gataatcgga aacatcgtcg taagccaagc gatcttttga ttcgacccaa
     2161 gcagaggtaa aggttgtctc tttaatgaga ttgccgttta aatcggtttc aataaagcca
     2221 acgagggcag ggcgtttttc attcgggaca agcgaacaga gatcgtctga gagttctcga
     2281 ggtaacattg gaatgttaaa gcccggcaaa tagttggtga aacaacgctg gcgagcggct
     2341 ttttcaatat gggaattttc tgggatataa gcggttgggt cagcaatcgc taccactaaa
     2401 cgccagccgg ttcgcttgcc attttgctta attggttcga tgaacagagc atcgtccata
     2461 tcttgcgtgc ttgggctgtc aatagtggta aagtaaagat gagataaatc ttcacgagct
     2521 aattcatcgt gtaattcata gtttttttca tttgcgaccg gttcacgagg ctgttcgtgg
     2581 cgggcaagtg tgacccacca aggggcaaaa ttatcagatt ctttacagat aaattgggta
     2641 acttgagcaa agaaaaaacg gtcgtcacgc aatgggtgcg tttttaattg tgcaaccacc
     2701 cagtcgccat tttctaattt ttcagtgact tttttatggg tatttgccgg gatagtttgc
     2761 ttaatgcttg ggtgatcaac cgcaagttgc aatttgccct ctttattcaa gcgaacttgg
     2821 gcaataaatc tatccagcat tggctcaagt agtgaatcaa tttctacctg ttctttgtcg
     2881 ccatcacgtt tgactacggc tttcactgaa tcaccgtgca ttactttttt catttccgca
     2941 ggtggaataa aatagctttt tttatcgcat tctaaaaaac cgaatgcttt gtctgaggct
     3001 ttgacaacgc ctgcgacata ttctttgctt gcttcaattt gttgtttgag ttgggctaaa
     3061 aggggattgt tttgaaacat aatttctcat tttaaaaata aaaaaaggtg ctatcgttgc
     3121 accgaaaaaa acaatggcat tataacggat ttgaaccaag ttcagcaaat aaatgctgtt
     3181 gctataactt ataaaagtta gtagaatata atccatttaa aaataatgga gacttttcaa
     3241 tgaaaaaatt acttgcaatt gcaacagtat tttctttagc agcctgttct actcaaacac
     3301 ttgaactatc tcctgctaag gaagctcaat atgatcgtgt tcaacacttc tttgtttcag
     3361 ggtttgctca aaaacaagag atagatgcag caaaaatttg taatggtgca gataaggtag
     3421 gtaaggtaga aactgaaact actttcttta atggcttgtt aggtacaatt acttatggta
     3481 tctattcacc tcgccaaatc cgtgtatatt gtaactaatc ctagtaattt aaggagttga
     3541 aaatgaaaaa attgcttgct atgtctgtat taacagccct gctgactaca gcatgtacaa
     3601 cacaaactgc ccatattggc ggcaaagtta ctgataatat taaaccaact aagagtgtaa
     3661 ctcagtcgtt ctttataggt gggattgggc aagaagaaac attgaatgtt gtagaggttt
     3721 gtggttccaa agataaagtg cagcaggttg aaacagtact ttcaggtggg gacattctat
     3781 taggtcttgt gactatcggt atttatacac ctcgtactgc aaatgtatat tgtaagtaat
     3841 attttattaa aaaaataacg gactcaattg agtccgttat ttttatagga attactcttc
     3901 gtcgcctaat tcacccattg ccattacgct gaagccggca tcaacgtgta acacttcacc
     3961 tgttacacca gaggctaaat ctgagcataa gaaagctgca gagttaccta catcatcaat
     4021 agttacggtg cggcgtaaag cagcggtttt ctcgaaagca gaaagcattt tcttgaagtt
     4081 tttaatgcct gaagcagcta atgtacggat cgggccggct gaaatcgcat ttacacgaat
     4141 accttcttta cctaaatcgg ctgccattac acgggttgca gcttctaaag aggctttggc
     4201 taagcacatt acattgtagt tagggattgc acgctctgca cctaagtaac ttaaggttaa
     4261 aagggtggca ttttcattta agaaggggcg agcagcttga gccattgcaa caaagctata
     4321 ggcactgatg tcgtgtgcaa tacgataacc ttcacgggtt gcggcattta cataatcgcc
     4381 gtctaattga tcgcccggtg caaatgcgat agcgtgaata aaaccgtcaa atttttccca
     4441 atgtttgctt aattcagtaa agcactcagt aatgctttca tcggttgcta catctaacgg
     4501 aaggacaatg tctgagccaa attctttggc aaattcttca actcgtggtt ttaatttatc
     4561 gtttaagtag gtgaatgcaa gttctgcacc ttgttgcttc attgcgtttg caataccgta
     4621 agcgatcgaa cggttgcttg ctaaacctgt cactaaaata cgtttaccag ttaagatacc
     4681 cataaaaatt cctcttttat attgattggg gcaaaattgt gtcgattata acggatatag
     4741 gaaaaacggg caaatggagt tagcggctca tcggatcaga aatccctttt tgaacgaggt
     4801 ctcaaaaagg gatttggtaa gataaattaa gcggtagttt gagcaataat attttgctgc
     4861 ggtttgattg ccccggctaa tttggctaat tcttccatac ggttaataat aatatatttt
     4921 ccttgtactg aaatcatacc gcttttttgg aaacgcccta ataaacggct gatagtttca
     4981 atggttaa
//
LOCUS       DANJWC010000163         4929 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.163, whole genome shotgun sequence.
ACCESSION   DANJWC010000163 DANJWC010000000
VERSION     DANJWC010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.163"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            69..1295
                     /locus_tag="PWX02_002104"
     CDS             69..1295
                     /locus_tag="PWX02_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDL1730075.1"
                     /translation="MAMPSQRFSLTWVLNLFGTAVGAGVLFLPINAGMGGFYPLIIMT
                     LLVGPMTYLAHRGLTRFVLSSKYKGSDITAVVREHFGEQAGKLITLLYFFAIFPILLI
                     YGVGITNTVSSFMENQLHIAPPSRAVLSFMLIAAMIGVMLLSEQVMLKITTCLVYPLV
                     LILLGLSIYLIPQWNTAALQQMPTTGDFLTTLWLTIPVLVFAFNHSPAISSFALSQQK
                     YYQDDKKAEIESAKVLRSTAFILVLFVMFFVFSCVLTLTPEELAQAKVQNISILSYLA
                     NKFDNPIISYFGPLVAFLAIGSSFFGHYLGAREGLEGLVNQMRKEPIEPSKFRKITAI
                     TFLIILWIVATINPSILGFIESLGGPIIAMILFIMPGYAVYKVPALARFKGEFGHLFV
                     LVMGCIAISAIVYGLL"
     gene            1352..2728
                     /locus_tag="PWX02_002105"
     CDS             1352..2728
                     /locus_tag="PWX02_002105"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDL1730076.1"
                     /translation="MISVFDMFKVGIGPSSSHTVGPMKAAKEFVDELQNRKFLNKIDR
                     LRVDVYGSLSLTGRGHNTDIAIIMGLMGYLPHNVDIERIEQTIENLKQNKQLIIAESS
                     VEPKVIAFDFYEDMPFHRDFLPRHENGVKFSALSGEELLFQNTYYSIGGGFIVDEANF
                     EQVKNSDLSVPFPYQNAADLLAHCKNNGLPLSSLVWKNELALRSRVEISDYLAMIWNT
                     MEACIERGLNTEGLLPGPLKVVRRASGLRRMLEANSKFNTDPMQIIDWVNMFALAVNE
                     ENAAGGRVVTAPTNGACGIVPAVLAYYQKFIAQLNQDTIERYLLTTSVIGSLYKMNAS
                     ISGAEVGCQGEVGVACSMAAAGLTEIMGGSAEQVCMAAEIAMEHNLGLTCDPVGGQVQ
                     VPCIERNAIASVKAINASRMALRRTSSPQVSLDKVIETMYETGKDMNAKYRETSTGGL
                     AIKILPCD"
     gene            complement(2783..4372)
                     /gene="purH"
                     /locus_tag="PWX02_002106"
     CDS             complement(2783..4372)
                     /gene="purH"
                     /locus_tag="PWX02_002106"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDL1730077.1"
                     /translation="MAIQQALLSVSDKKGIVEFARGLSARGVKLLSTGGTAKLLAEAG
                     LLVTDVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGTDDEVMNQHGIERIDMVVVNLY
                     PFAQTVANPNCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVNNNDFDAILAEMDQHQ
                     NSLTLETRFNLAIKAFEHTAQYDSMIANYFGQKVPPYQGAEEEDLTQPSGQFPRTLNL
                     NFVRKQTMRYGENSHQNAAFYVENELKEASVSTAKQLQGKALSYNNIADTDAALECVK
                     EFSEPACVIVKHANPCGVALGKNILDAYNRAYQTDPTSAFGGIIAFNRELDGETAQTI
                     ADRQFVEVIIAPTVSASAKEVLATKKNVRVLECGEFSEAVKRLDFKRVNGGLLVQDAD
                     QGSVSIDDLKVVSERKPTEAELKDLLFCWKVAKYVKSNAIVYAKDGQTIGIGAGQMSR
                     VYSAKIAGIKAEDEGLQVAGCVMASDAFFPFRDGIDAAAKVGITCVIHPGGSMRDQEV
                     IDAANEHGMAVVLTGMRHFRH"
     gene            4544..4813
                     /locus_tag="PWX02_002107"
     CDS             4544..4813
                     /locus_tag="PWX02_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL1730078.1"
                     /translation="MINHNLADFVFEYFTENNLKAGTLRYRYIKENVIDAYSTRVDDA
                     FQGKGIAGELYNALIAFAQEKGLKIKPSCSYIEVKMQRSHRDLIA"
BASE COUNT         1380 a          962 c         1039 g         1548 t
ORIGIN      
        1 tgttgtgtta ctgtttccca tcgctaatat agcatagctt gttagataat tctttaaagg
       61 agattactat ggctatgcca tctcaacgtt tttctcttac ttgggtacta aatttatttg
      121 gtactgcggt aggagccggc gttttatttt tgccaattaa tgcaggtatg ggtgggttct
      181 acccacttat tattatgact ttgttagttg gaccaatgac atatttagct caccgtggct
      241 taacacgttt tgtgctttct tcaaaataca aagggagcga tattacggct gttgttcgtg
      301 aacattttgg tgaacaggcc ggtaaattga tcacgctact gtacttcttt gccatttttc
      361 cgattttact gatttatggt gtaggtatta ccaataccgt gagttctttt atggagaatc
      421 agcttcatat cgcgccacca tctagggctg tattatcgtt tatgctaata gctgctatga
      481 tcggagtaat gttattaagt gagcaagtca tgttaaaaat cacaacttgt cttgtgtatc
      541 cattagtgtt gattttattg ggcttatcta tttatttaat tccacagtgg aatacagctg
      601 ccttacagca aatgccaact acaggagact tccttactac actttggctg actattcctg
      661 ttttagtctt tgcttttaac cattcgccgg ctatttcttc ttttgcactt tctcaacaga
      721 aatattacca agatgacaag aaggctgaaa ttgaatcagc taaagtatta cgttctaccg
      781 cctttatctt ggtgttattt gtgatgttct ttgtatttag ctgtgtatta actttgacac
      841 ctgaagaatt agcacaagct aaagtgcaaa atatttcgat tctttcttac cttgcgaata
      901 aatttgataa tccgattatt tcctattttg gaccgttggt tgcttttttg gcgattggta
      961 gctctttctt tgggcattat ttaggtgcgc gcgaggggtt agaaggattg gttaatcaaa
     1021 tgcgtaaaga gccgattgag ccaagcaaat tccgcaaaat taccgctatt acttttttga
     1081 tcattttatg gattgttgcg actatcaatc caagcatttt aggcttcatt gaaagccttg
     1141 gtgggccgat tattgcgatg atcttgttta ttatgcctgg ctatgccgta tataaagtac
     1201 cggcacttgc acgttttaaa ggtgaatttg gccatttatt tgtgcttgtt atgggttgta
     1261 ttgctatttc agcaatagta tatgggttac tgtaatcttt ctaaaatcaa tgtagaatac
     1321 gcccttttat tttctatgtg aatttgtgtt tatgattagt gtatttgata tgtttaaagt
     1381 ggggattggg ccttccagtt cccatactgt ggggccgatg aaagcggcaa aggaatttgt
     1441 tgatgagcta caaaatcgca aatttttaaa caaaattgac cgcttgcggg tagatgtcta
     1501 tggttcatta tcactcaccg ggcgaggaca taatacggat attgccatca taatgggatt
     1561 aatggggtat ttgcctcata atgtggatat tgaacgtatt gagcagacga ttgaaaatct
     1621 aaaacaaaat aagcaactta tcattgctga aagcagtgtt gagcctaaag tgattgcgtt
     1681 cgatttttat gaggatatgc catttcatcg agatttctta cctcggcacg aaaatggtgt
     1741 gaaatttagt gccttgagcg gtgaagagtt gctatttcaa aacacctatt attctattgg
     1801 tggtggcttt attgttgatg aagctaactt tgagcaagtt aaaaattcag atctttctgt
     1861 tccattccca tatcagaatg cagcagattt acttgcacat tgtaagaata acgggttacc
     1921 gctttcttct ttagtctgga aaaatgagct tgctttacgc agtagagtag aaatttcgga
     1981 ttatttggca atgatttgga atacaatgga agcctgtatt gagcgtggtt tgaatacgga
     2041 agggttacta cccgggcctc tgaaagtggt acgccgtgcc agtgggctac gcagaatgtt
     2101 agaggcaaac agtaaattca ataccgaccc aatgcaaatt atcgattggg tgaatatgtt
     2161 tgcccttgcc gtgaatgaag aaaatgcggc aggtgggcga gtagtcaccg cacctaccaa
     2221 tggagcctgt ggtattgttc cggctgtgtt ggcgtactac caaaaattta ttgcacagct
     2281 taatcaagat actattgagc gttatttatt gactaccagt gtgattggtt cgttatacaa
     2341 aatgaatgcc tctatttccg gtgctgaagt aggttgccaa ggggaagttg gtgttgcttg
     2401 ttcaatggcg gcagcaggtt taacggaaat tatgggtggc agtgctgaac aggtgtgtat
     2461 ggctgcagaa attgcaatgg agcacaattt aggcctaact tgtgaccctg ttggcggaca
     2521 agtgcaagtg ccttgtattg aaagaaatgc aatcgcttca gtcaaagcga ttaatgccag
     2581 cagaatggca ctacgccgta cttcttcgcc tcaggtaagt ttagataaag tcattgaaac
     2641 aatgtacgaa accggcaaag atatgaatgc aaaataccgt gaaacctcaa ccggtggctt
     2701 agcaatcaaa attttacctt gtgattaatt aatgaaaaag gctaccttat caaaataagg
     2761 tagcctttta gtttgtagaa cattaatgac ggaaatgcct cattccggtt agtaccactg
     2821 ccataccgtg ttcgttggcg gcatcaatca cttcttgatc acgcattgaa ccacctgggt
     2881 gaatcacaca agtaatccct acttttgcag cagcgtcaat accatcacgg aacgggaaga
     2941 acgcatcgga agccattaca caaccagcaa cttgtaagcc ctcgtcttcc gctttaatac
     3001 ctgcaatttt tgcggaataa acacggctca tctgccctgc accgataccg atagtttgcc
     3061 cgtctttggc atacacaatc gcattcgatt tcacatattt cgccactttc cagcagaaca
     3121 ataggtcttt tagctcagct tcagttggtt tacgctctga aaccaccttt aaatcgtcaa
     3181 tagatacaga gccttggtcg gcatcttgca ctaataatcc gccgtttacc cgtttgaaat
     3241 ctaagcgttt tacagcttct gagaactcgc cacattcaag cacacgcacg tttttcttag
     3301 tggctaacac ttctttggca gaagcagaaa cggttggtgc aataatcact tcaacaaatt
     3361 gacggtctgc gatggtttga gcagtttcgc cgtctaattc acggttaaag gcaataatgc
     3421 caccgaaagc agaagttgga tcggtttggt aagcacggtt gtaggcatct aaaatatttt
     3481 tacccaaagc aacaccacaa gggttagcgt gttttacaat cacacaagca ggctcgctaa
     3541 attctttcac gcattcaaga gccgcatcgg tatctgcaat attattatac gaaagggctt
     3601 taccttgtaa ctgtttggcg gtggaaacag aggcttcttt taactcattt tcgacataga
     3661 atgcagcgtt ttgatgcgaa ttttcgccat aacgcatcgt ttgtttacgc acaaaattga
     3721 ggtttaaggt gcgagggaat tgtccacttg gttgggttaa atcttcttcc tcagcccctt
     3781 gataaggcgg cactttttgc ccgaaatagt tggcaatcat tgaatcgtat tgtgcggtat
     3841 gctcaaacgc tttaatagca agattaaaac gggtttctaa agttaaactg ttttgatgtt
     3901 gatccatttc ggcaagaata gcatcaaaat cattgttatt gaccacaatc gccacatctt
     3961 tatgattttt cgctgcagaa cgcaccattg tcggaccacc gatatcaata ttttccaccg
     4021 catcttcaag gctacaattt ggattggcaa cggtttgagc aaaagggtag aggtttacta
     4081 ccaccatatc aattcgttca atgccgtgtt ggttcatcac ttcatcatca gtaccacggc
     4141 gacctagaat tccgccgtgc actttcgggt gcagggtttt tacacgcccg tccatcattt
     4201 caggaaagcc ggtgtaatct gatacatctg ttactagcaa accggcttca gccagtaatt
     4261 ttgcggttcc gcccgtagaa agtaatttta ccccacgagc cgatagccca cgagcaaatt
     4321 cgacaatgcc ttttttatca gacacactta ataacgcctg ttgaatcgcc attcttaaat
     4381 tccttaaaat gagtgttaac gtgaaaataa acggcaaaga ttataacgca aacgtttgct
     4441 tttttcatct acaagtggtc gattttgcaa atttttctgc aaaatcgacc gcttgcactc
     4501 gtgattaaag cgggtagaat agaggaaaaa aatcggagga actatgatta accacaattt
     4561 agcagatttc gtatttgagt attttaccga aaacaaccta aaagcaggca cactacgtta
     4621 tcgctacata aaagagaacg tgattgatgc ttacagtacc cgagtagatg atgcctttca
     4681 aggtaaaggt attgcaggag agctttacaa tgctttaatt gcgtttgcgc aagaaaaagg
     4741 gctgaaaatc aagccaagtt gtagttatat tgaggtaaaa atgcaacgaa gtcatcgaga
     4801 tttaattgct tagagaggag caagcggtcg taaaatttca aaaatttacg accgctgtct
     4861 tattcaattc tcaaaaatcc gtcacacaaa atataagccc tattcattaa acaacgccat
     4921 ataaaattt
//
LOCUS       DANJWC010000164         4836 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.164, whole genome shotgun sequence.
ACCESSION   DANJWC010000164 DANJWC010000000
VERSION     DANJWC010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.164"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..1078
                     /gene="serC"
                     /locus_tag="PWX02_002108"
     CDS             <1..1078
                     /gene="serC"
                     /locus_tag="PWX02_002108"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HDL1730079.1"
                     /translation="EIYNFSAGPAMMPKKVLEKAQQELLNWLDQGTSVMEVSHRGKLF
                     MELAAQSEADLRKLYNVPDNYRILFLQGGARGQFAAIPMNLIGKKGKALYLNSGHWSA
                     TAAKEARNFAEIDEINILETEPQLKVSRLDFSDIAEQYDYVHYCTNETISGVEIFDIP
                     NVGNTPLVADMSSNILARGIDINKFGVIYAGAQKNLGPAGITIVIIRDDLIGNARKDT
                     PSIWNYATQRDADSMINTPPTFAWYLCSLVFQHLLAEGGLKATEERNLAKAMLLYEYL
                     DSSKFYYNTVAHENRSLMNVTFTTGNDELNAKFVAEATACGLQALKGHKVLGGMRASI
                     YNAMPIDGVKALIEFMKAFEQCNA"
     gene            complement(1135..2112)
                     /locus_tag="PWX02_002109"
     CDS             complement(1135..2112)
                     /locus_tag="PWX02_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDL1730080.1"
                     /translation="MTLAVAAIIGGLIILVWSADRFVEGAASTARHFGMTPLLIGIVV
                     IGFGTSAPEMLVSASSALSGSPGIALGNAYGSNITNIALILGLTAIIKPLSVNSDVLK
                     KELPILLAVTLLSAYLVYDANVTQLDAIILLAVFFVYMAWTIITAMKSKNDALSLDVD
                     AELTNSAKMGLGKSILWLIIGLVSLVISSQFLVWGAVEVAKFFGVSDLVIGLTIVAVG
                     TSLPELASSIAAARKGEVDLALGNIIGSNLFNTLAVVGIAGAIEPMQASAEVFSRDVL
                     IMSALTFLLFIFGINLYRRTEGGRINRLEGLLLFAAYVGYNFYLFKTAV"
     gene            2224..4791
                     /gene="glnD"
                     /locus_tag="PWX02_002110"
     CDS             2224..4791
                     /gene="glnD"
                     /locus_tag="PWX02_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HDL1730081.1"
                     /translation="MMKNSNELKTLLSAFTEQQRQAFQSENVLSLLAQRSQFYDQLLQ
                     ALWQDFGLAQYEHITLLAVGGYGREEMFPLSDLDILVLTEKPLEEAIQQRLNELFALL
                     WDSKLQLGTSVRTLEECIQIGKAEISVATNMLEGRFLLGNKSLWLRLKEALYQPDFWD
                     IKAFFRAKMAEKNERYARYHNTSYNLEPDLKHSPGGLRDLHLISWIMLRHYGVSSFVD
                     LLNKGLLYPEEYKELIAAQAVLFRMRFALHLQLKRYDNRLRFDRQLQLSEILGYQGEA
                     NQAVELMMRDYFQATKSISQLSQLLLNSFEQAYVNELQKNAQKQPLDQYFYRQEQQLF
                     VVERKIFKTQPTTMLDLFFHLTNDPTLIANADTLRQLRLCLQQQHEPMCFLPEMREKF
                     VQLFAQPKVISRAIVPMHQLGFLSAYLPQWKGIEGLMQFDLFHIYTVDEHTVRVMLNL
                     ENFSEDPSRAFFPLCHQLFATLSEKPLLYLAALFHDIGKGREGEHAQVGALDMREFAE
                     LHGFSQAQINDMVWLVAEHLTMSITAQRRDIHDPQIISEFAKKVQNQTALSSLLCLTV
                     ADICATSESLWNDWKASLFTQLYQFTLQQLAQNLNYQAVAQEHRLQALELMKFVLSAD
                     ERIALNAFWQPCPERYFLHHTPTQLVWHALHYVKTRRLPMVLISNEYARGGTEIFVCC
                     QDQPQLFARIAQLLSQKRISIHDAQIITTENGVVLDSFIVSERDGDDDLTDERCMQIQ
                     QAMMKMLSLTETGIKFTKKPVKHLSFKRKTRLRFLPPSSKQQTEFELFTLDREGLLAQ
                     IGYIFNRLRLNLINAKITTIGERVEDFFVVSNDQGKALSEQEQLELKAILITELDSE"
BASE COUNT         1556 a          948 c         1004 g         1328 t
ORIGIN      
        1 agagatatat aacttcagtg ccggcccggc aatgatgccg aaaaaagtac tcgaaaaagc
       61 gcaacaagaa ttattaaatt ggctagacca aggcacatca gtgatggaag tcagccaccg
      121 tggaaagcta tttatggagc tggcggcaca atctgaagca gatttacgca agctgtataa
      181 tgtgccggac aattatcgta ttctatttct tcaaggtggt gcaagggggc aatttgcagc
      241 cattccaatg aatttaatcg gtaaaaaagg caaagcgctt taccttaatt cagggcattg
      301 gtcggcaaca gcagcaaaag aagcacgtaa ctttgcggaa attgatgaaa tcaatatttt
      361 agagactgaa ccgcaactta aagtgagtcg tttagatttc agcgatattg cagagcaata
      421 tgattatgtc cattattgca ccaatgaaac catcagtggg gtagaaattt tcgatattcc
      481 gaatgtcgga aataccccgt tagtggcaga tatgtcgtct aatatcttag cccgagggat
      541 tgatatcaac aaatttggag taatttatgc cggagcgcaa aaaaatctag ggcctgctgg
      601 cattacgatt gtgattattc gtgatgattt aattggcaat gcacgcaaag ataccccatc
      661 tatttggaat tatgcaactc agcgagatgc tgattcgatg attaataccc cgccaacttt
      721 tgcttggtat ttatgttctt tagtgttcca acatttactg gcagagggtg gacttaaagc
      781 tactgaagaa cgtaatcttg ctaaagctat gttgctttat gaatatttgg atagctctaa
      841 attctattac aatacggtag cacacgaaaa tcgttccttg atgaatgtga cttttaccac
      901 tggtaatgat gagctaaatg caaaatttgt ggcagaagcg accgcttgtg gtttacaggc
      961 attgaaaggc cataaagtat taggtggaat gcgtgcctca atttataacg caatgccgat
     1021 tgatggggtg aaagcactca ttgaatttat gaaggcattt gaacaatgta atgcttaatt
     1081 caagcttaaa catataaatt aaagagccac gtttgtggct ctttgaattt ggtactagac
     1141 cgcagtttta aacaaataga aattatagcc tacataagcg gcaaagagta ataagccctc
     1201 taaccggtta attctgccac cttctgtccg acgatagagg ttgattccga aaatgaatag
     1261 taagaaggta agggcagaca ttatcaaaac atctcgagaa aacacttccg ctgaagcttg
     1321 cattggttct atagcaccgg caatccccac cacggctaac gtattaaata agttagaacc
     1381 gataatgtta cctaaagcta aatccacttc tcctttacga gcagctgcaa tagaagaggc
     1441 taattcaggt agtgaggtgc ctaccgccac aatagtaagc ccgattacca aatcactcac
     1501 accaaaaaat ttagccactt caactgctcc ccagactaag aattgggaac taattactag
     1561 cgatactaaa ccaataatta gccaaagaat ggattttcct aatcccatct tagcactatt
     1621 ggttaattct gcatcaacat ctaagcttaa tgcatcattt ttacttttca ttgcggtaat
     1681 aattgtccac gccatatata caaaaaacac agcaagtaaa ataatcgcat caagttgagt
     1741 gacgtttgca tcataaacca aataagcaga aagcagggtg acagccagta aaatcggcaa
     1801 ctcttttttt aacacatcgg aattaactga tagtggttta attattgcgg ttaaacctaa
     1861 aatcagcgca atattggtaa tgttagaacc gtaggcatta ccgagagcga tacccggcga
     1921 tccgcttaat gcagaagatg cggatacgag catctccggt gcagatgtac caaaaccaat
     1981 gaccacaata ccgattaaca atggtgtcat accaaaatgg cgagccgtag aggctgcccc
     2041 ttctacaaaa cgatccgcac tccaaactaa tataattaag ccaccgataa tagcggcaac
     2101 agcaagtgtc ataaactatc cttttagatg aaaaacataa aggcattata agcattttag
     2161 ccaaaataaa tataggcaaa cgaaaataaa tgacctatta ttttgccagt ttaggaagag
     2221 attatgatga aaaacagcaa cgaattaaaa acgcttttat cagcatttac ggagcaacag
     2281 cggcaagcat ttcaatccga aaatgtactc agcttacttg cacaacgtag ccaattttat
     2341 gaccaactac tgcaagcact ttggcaagat tttggactcg ctcaatatga acatattacc
     2401 ctattagcgg taggggggta cggacgtgag gagatgtttc cgctttcgga tctggatatc
     2461 ttagtgttaa ctgaaaaacc gctagaagag gctattcagc agcgtttaaa cgaactattt
     2521 gccttgcttt gggacagcaa actgcaactt ggcaccagtg tgcgtacgct cgaagaatgt
     2581 attcagattg ggaaagcaga aatttcggtt gccactaata tgttggaagg gcgattttta
     2641 ttgggtaata aatcgctttg gctaagatta aaagaagcac tttatcagcc ggatttctgg
     2701 gatattaaag cgttttttag agcgaaaatg gcggaaaaaa atgagcgtta tgcacgttat
     2761 cataatacta gctataacct tgagccagat ctcaaacata gcccgggagg attgcgagat
     2821 ctgcatttaa tctcttggat tatgctacgg cattatggtg tatcttcttt tgtcgatctc
     2881 ctcaataaag ggctacttta cccggaagaa tataaggagc tgatcgcagc acaagcggta
     2941 ctttttcgga tgcgttttgc acttcatttg caactgaaac gttacgataa tcgccttcgg
     3001 tttgacagac aattacaact gagtgaaata ttaggctacc aaggcgaagc aaatcaagcg
     3061 gtggaattaa tgatgcgaga ttattttcaa gccacaaaat ctatttcgca actgagccaa
     3121 ttattgttaa acagttttga acaagcctat gtaaacgagt tgcaaaaaaa tgcacaaaaa
     3181 caaccgcttg atcagtattt ttatcggcaa gaacagcaat tgtttgtggt cgaacgcaaa
     3241 atatttaaaa cccaacccac aacaatgttg gatctctttt ttcatcttac taatgaccct
     3301 acgttaatag cgaatgccga caccttacgt cagttgagac tgtgtttaca gcaacaacac
     3361 gagcctatgt gctttttgcc tgaaatgcga gagaaattcg tacagctttt tgctcaaccg
     3421 aaagtcataa gccgagcaat agttccaatg caccaactgg gttttttaag tgcttatcta
     3481 ccgcaatgga aggggatcga agggctaatg caattcgacc tattccatat ttatacagtc
     3541 gatgagcata cggtcagagt catgttaaat ttagaaaatt ttagcgaaga tccaagtcgg
     3601 gcgtttttcc cactatgtca tcagctattt gcaacgcttt ctgagaaacc gttgctctat
     3661 cttgcggctc ttttccacga tattggcaag ggtagagagg gcgaacacgc tcaagttggt
     3721 gcgctggata tgcgagaatt tgcggaacta cacggttttt ctcaagcaca aatcaatgat
     3781 atggtttggc tggtggcaga gcatttgaca atgtcgatca ccgcacaaag acgagatatc
     3841 cacgatccgc aaatcatcag cgaatttgca aaaaaagtgc aaaatcagac cgctttatct
     3901 tcactacttt gtttaacggt tgcagatatt tgtgcaacca gtgagagcct ctggaatgat
     3961 tggaaagcct cgctgtttac ccaattatat caatttacgc tacagcaact ggctcaaaat
     4021 ttgaattatc aagcagtggc acaagagcat cgcttgcaag cattagaact gatgaagttc
     4081 gtgctttcag ctgatgagcg aatagcatta aacgcatttt ggcaaccttg cccggagcgt
     4141 tattttcttc accatacacc gactcagttg gtatggcacg ctctgcatta tgtaaaaact
     4201 cgccggttgc cgatggtttt aatcagtaat gaatatgccc gtggtggtac agaaattttt
     4261 gtatgttgcc aagatcaacc tcaactgttt gctcgaatcg cccaattact cagccaaaaa
     4321 cgcattagca ttcacgatgc acaaattatt actactgaaa atggtgtagt gttagacagc
     4381 tttattgtaa gtgagcgtga tggtgatgat gatttaaccg atgagagatg tatgcaaatt
     4441 cagcaggcga tgatgaaaat gttaagctta actgaaacag gcataaaatt taccaaaaaa
     4501 ccggtgaaac atttgtcatt taagcgtaaa actcggctgc gttttcttcc tccgtcatca
     4561 aagcaacaaa ccgaatttga actttttacc ttggatagag aagggttgtt ggcacaaata
     4621 ggctatattt ttaaccggct ccgattaaat ttaattaatg cgaaaattac gactattggt
     4681 gaaagggtag aggatttctt tgtggtcagt aatgatcaag gaaaagcttt atcggaacaa
     4741 gaacagttag aactaaaagc cattctaatt accgaactag atagtgaatg aaaacaaccg
     4801 cttggagtga agactgcaag cggtaaattt ttcaac
//
LOCUS       DANJWC010000165         4631 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.165, whole genome shotgun sequence.
ACCESSION   DANJWC010000165 DANJWC010000000
VERSION     DANJWC010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.165"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..182
                     /locus_tag="PWX02_002111"
     CDS             <1..182
                     /locus_tag="PWX02_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HDL1730082.1"
                     /translation="LGAKSPTPEWGAMIRESMELIYIAPWTVILPGLAIIFSIFTVTI
                     LGTRLSVIFEKYRKE"
     gene            253..1305
                     /locus_tag="PWX02_002112"
     CDS             253..1305
                     /locus_tag="PWX02_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1730083.1"
                     /translation="MALLDIRHLSIEIDTPKGRVKMVDNINLTVDEGEICGLVGESGS
                     GKSLIAKVICGMIKDEWIVSADRFRFDDIELLKLSPAERRKLVGDHISMVFQDPLTSL
                     DPSQQIGKQLMQTIQFRGKWWQRFGWKKKKAIELLHRVGIRDHQDIMKSYPSDITEGE
                     AQKVMIAMAVANQPRLLVADEPTNIMESTTQLQVYRLLSSMNQNLGTSILLVSNDMCS
                     IQNWVDSYNVLYCGQTVEIADKESILESSYHPYTRVLLNSSPDFSQPLAFKSSLNTLR
                     GSVPMLQEMPIGCRLGPRCPFAQKKCINKPPLVKVKQHEFACHFPINFREQNLLKNKT
                     ENTPFVVAEIAEKEAF"
     gene            complement(1314..1544)
                     /locus_tag="PWX02_002113"
     CDS             complement(1314..1544)
                     /locus_tag="PWX02_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDL1730084.1"
                     /translation="MKLTACKLVFLLYLMFNLTACGTVLSLVEQDYRVYAGVIKDFQV
                     MQEGGIFAVLAVIDLPLSFVLDTLMLPVTLSQ"
     gene            1674..2756
                     /gene="leuB"
                     /locus_tag="PWX02_002114"
     CDS             1674..2756
                     /gene="leuB"
                     /locus_tag="PWX02_002114"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDL1730085.1"
                     /translation="MQTYNIAVLAGDGIGPEVMAEAIKVLEATQQKFGFKLNFNQYNI
                     GGAAIDAQGKALPENTLKGCEEADAILFGSVGGPKWTHLPPDEQPERGSLLPLRKHFS
                     LFCNLRPAALYQGLEKFCPLRADIAAKGFDMVVVRELTGGIYFGQPKGRDGEGAQTRA
                     FDTEVYYKYEIERITRVAFESAMKRKKHVTSVDKANVLQSSILWRETVTEVAKEFPEV
                     TLEHMYIDNATMQLIKAPESFDILLCSNIFGDIISDEAAMITGSMGMLPSASLNEQGF
                     GLYEPAGGSAPDIAGKNIANPIAQILSAAMMLRYSFNLGDAADAIETAIQKALADGYR
                     TADLADDSKPLSTSEMGDIIAKNILA"
     gene            complement(2843..3238)
                     /gene="msrB"
                     /locus_tag="PWX02_002115"
     CDS             complement(2843..3238)
                     /gene="msrB"
                     /locus_tag="PWX02_002115"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDL1730086.1"
                     /translation="MIKPIHALTEQQVDILINHGTERPFTGKFLNEHRVGIYRCARCH
                     NDLFHSDTKFDAGCGWPSFYQTISESSLRYLDDYSLGRHRIEIRCGNCDSHMGHVFPD
                     GPPPTGLRFCLNSAALNFKWEETGEEIDG"
     gene            3463..4467
                     /gene="gap"
                     /locus_tag="PWX02_002116"
     CDS             3463..4467
                     /gene="gap"
                     /locus_tag="PWX02_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010129843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL1730087.1"
                     /translation="MAIKIGINGFGRIGRIVFRAAQHRDDIEVVGINDLIDVDYMAYM
                     LKYDSTHGRFDGTVEVKDGQLVVNGKAIRVTAERDPANLKWNEIGVDIAVEATGLFLD
                     DETARKHITAGAKKVVLTGPSKDATPMFVNGVNFDTYAGQDIVSNASCTTNCLAPLAK
                     VIHEKFGIKEGLMTTVHATTATQKTVDGPSAKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPALNGKLTGMAFRVPTTNVSVVDLTVNLEKPATYAEICAEIKRASENEMKGVLGYTE
                     DAVVSTDFNGATETSVFDAAAGIALTDTFVKLVSWYDNEVGYSNKVLDLVAHVYNYKG
                     "
BASE COUNT         1436 a          873 c          999 g         1323 t
ORIGIN      
        1 tgctgggggc taaaagcccg acccctgaat ggggagcgat gattcgtgaa tccatggaac
       61 tgatttatat agctccttgg acggtgatat tacctggctt agcaattatt tttagtattt
      121 ttaccgttac aatacttggt acacgcttaa gcgtgatttt tgaaaaatac cgtaaagaat
      181 agtatttaaa taggatactg acacataaca agcggtcatt tttaagtaaa attttacaga
      241 ttaaggaata gtatggcatt acttgatatt cgccatttaa gtattgaaat tgatacgcca
      301 aaaggcagag taaaaatggt ggataacatc aacctgactg ttgatgaggg cgaaatttgt
      361 ggcttggtgg gagaatctgg ttcgggaaaa agcctcatag ccaaagtgat ttgcggtatg
      421 attaaagatg aatggattgt gagtgcagat cgctttcgct ttgacgatat tgaattactg
      481 aaactttcgc cagcggaacg ccgaaaattg gtaggcgatc atatttcaat ggtattccaa
      541 gatccgctta ctagccttga cccaagccag caaattggta aacaactgat gcaaaccatt
      601 caatttagag ggaagtggtg gcaacgattt gggtggaaaa agaaaaaagc aatcgagcta
      661 ctacaccgtg ttggtattcg tgatcatcaa gatattatga aaagttaccc aagcgatatt
      721 accgaaggcg aagctcagaa agtgatgatt gcaatggcgg tggcaaatca gccccgatta
      781 ttggtggcag atgagccgac taatattatg gaatcaacca cgcagttgca ggtttatcgc
      841 ttgctttcca gtatgaatca gaatttggga acctcaatct tgctggtgag taatgacatg
      901 tgtagcattc aaaattgggt tgattcttac aatgtgcttt attgtggaca aaccgttgaa
      961 attgcggata aagagagcat tcttgaatcg tcttaccacc cctatactcg agtgttactc
     1021 aatagttcgc cggacttttc tcagccttta gcctttaaaa gtagcttaaa tacgctacga
     1081 ggctcagtcc cgatgttaca agaaatgccg attggttgtc gtttaggccc tcgttgccct
     1141 tttgcacaaa aaaaatgtat caacaaaccg ccattagtga aagtaaaaca gcacgagttt
     1201 gcctgccatt ttccgattaa ctttagagaa caaaatttgc ttaaaaataa aacggaaaat
     1261 acgccgtttg tggtggctga aatagcggaa aaagaggcgt tttaatgtgg ttattattgg
     1321 ctaagtgtaa cgggtaacat taaagtatct aatacgaaac tcaagggtaa atcgatcacc
     1381 gcaagcaccg cgaatatacc gccttcttgc attacttgga aatcttttat tacgccggca
     1441 taaacacggt aatcctgttc aaccaagctt agtactgtgc cacaagcggt gaggttaaac
     1501 atcaaataga gtagaaaaac gagtttacaa gcggtcaatt tcatcaaaaa atctccaaat
     1561 acgcttttat tttacgctat ttttatcggg ttggcgtaaa attcgtttta aaaacagcta
     1621 ttttcaggca gaatgtagcc gaatcgacat taaatatact aagaggatct tatatgcaaa
     1681 cttacaacat tgcagtgctt gccggtgacg gcattgggcc ggaagtgatg gcagaagcta
     1741 tcaaagtgct tgaagcgact caacaaaaat ttggctttaa actaaatttt aatcaatata
     1801 acattggtgg agcagcgatt gatgctcaag gtaaagcgtt acccgaaaat accttaaaag
     1861 gctgtgaaga ggcagatgcg attttgtttg gctcggtagg tggcccgaaa tggacgcatt
     1921 taccacctga tgagcaacct gaacgaggtt cgctattacc actgcgtaaa catttctcgc
     1981 tattctgtaa tttacgccct gctgcattgt atcaagggct ggaaaaattc tgcccattac
     2041 gtgccgatat tgctgctaaa ggttttgata tggtggtggt gcgtgaatta accggcggga
     2101 tttattttgg acagccgaaa ggccgtgatg gtgaaggtgc gcaaacgaga gcgtttgaca
     2161 ccgaagtata ctacaaatat gaaatcgagc gtattacccg tgtggcgttt gaatctgcaa
     2221 tgaaacgtaa aaaacacgta acctctgtgg ataaagccaa tgtgttacaa agctcaattt
     2281 tatggcgtga aaccgtcacg gaagtagcta aagagttccc agaagtcacg cttgagcata
     2341 tgtatattga taatgcgaca atgcagctaa tcaaagcacc tgaatcgttc gatattttat
     2401 tatgctcaaa catcttcggc gatattattt ccgatgaggc tgcaatgatt acaggttcta
     2461 tgggcatgtt gccttcagcc agcctcaatg agcaaggttt cggcttatat gaaccggcag
     2521 gcggttcagc accggatatt gccggtaaaa atattgcgaa tccgattgcg caaattttat
     2581 cagccgcaat gatgttgcgt tatagcttta acttaggtga tgctgctgat gcgattgaaa
     2641 cagcgatcca aaaagccctt gctgatggct accgtaccgc tgatttagct gatgacagca
     2701 aaccgctttc tacctcagaa atgggcgata ttattgcgaa aaatattctc gcttaaggct
     2761 agaagttaaa tagcacaagc ggtcattatt cgccaaattt ttgcaaattt tccgctaata
     2821 atgaccgctt gtttattact cgttagccgt caatctcttc gcctgtttct tcccacttaa
     2881 aattcaatgc cgcagagttg aggcaaaaac gcaaccctgt tggtggtggg ccgtccggaa
     2941 aaacgtgtcc catatgcgaa tcacaattcc cacaacgaat ttcgatacga tgacgtccta
     3001 agctgtaatc atctaagtaa cgtagtgacg attctgaaat cgtttggtaa aaacttggcc
     3061 agccacaacc ggcatcaaat ttagtatctg agtgaaataa atcgttatgg catctggcac
     3121 aacgataaat tcctacccga tgttcattca aaaattttcc ggtaaacgga cgttccgtac
     3181 cgtggttgat taaaatatca acttgttgtt ctgtcagggc gtgaataggt ttaatcatca
     3241 gcaattctcc taagatagta gcgaagtcat tgtagctttt tttgacctga aaatcgttat
     3301 aaatttcaaa atttgacata gatcacaaaa aatttatctg ttatattgga agggttcttt
     3361 tggcttgtca ggaaagtgaa aagatgtgct ataattcaag catctttagt gtagtacata
     3421 aagaacaaat tttgtttaac tttaatatag gtagaaaaat ctatggcaat taaaattggt
     3481 attaacggct ttggccgtat cggtcgtatc gttttccgtg cagcacaaca ccgtgatgac
     3541 atcgaagtgg taggtatcaa cgacttaatc gatgttgatt atatggctta tatgctgaaa
     3601 tatgattcaa ctcacggtcg ttttgacggt actgttgaag ttaaagacgg tcaattagta
     3661 gttaacggta aagcgatccg cgtaacagct gagcgtgacc cagctaactt aaaatggaat
     3721 gagatcggtg ttgatatcgc agttgaagca acaggtttat tcttagatga tgaaactgct
     3781 cgcaaacaca tcaccgcagg tgcgaagaaa gttgttttaa ccggtccgtc taaagatgca
     3841 acacctatgt tcgtaaacgg tgtaaacttc gatacttatg caggtcaaga catcgtatcc
     3901 aacgcttctt gtacaacgaa ctgtttagca ccattagcta aagttattca cgaaaaattc
     3961 ggcattaaag aaggtttaat gacaactgtt cacgcaacta ctgcaacgca aaaaacagta
     4021 gatggtccat cagcgaaaga ctggcgtggt ggtcgtggcg catcacaaaa tatcattcct
     4081 tcatcaacag gtgcagcgaa agcagtaggt aaagtattac ctgcattaaa cggcaaatta
     4141 accggtatgg ctttccgtgt tccaaccact aacgtttctg ttgttgattt aactgtaaac
     4201 ttagaaaaac cagcaactta tgcagaaatc tgtgctgaaa tcaaacgtgc ttcagaaaac
     4261 gaaatgaaag gcgtattagg ctacacagaa gatgcagttg tttcaacaga cttcaatggt
     4321 gcaactgaaa cttcagtatt tgatgcagca gcaggtatcg cattaactga tactttcgtt
     4381 aaattagtat cttggtacga taacgaagtt ggctactcaa acaaagtatt agacttagtt
     4441 gctcacgtat ataactacaa aggttaattg ctaagttata ttttaaatat agagccaaat
     4501 tctgaacgga atttggctct tttgctgcga acaataaatt gaatatagtc gcgttttccc
     4561 ataaaatcaa tttcctacaa acaggaacta agcacagtaa ggcagatttt cttgctcttt
     4621 gcagtttatt t
//
LOCUS       DANJWC010000166         4590 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.166, whole genome shotgun sequence.
ACCESSION   DANJWC010000166 DANJWC010000000
VERSION     DANJWC010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.166"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..1739)
                     /gene="selB"
                     /locus_tag="PWX02_002117"
     CDS             complement(<1..1739)
                     /gene="selB"
                     /locus_tag="PWX02_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDL1730088.1"
                     /translation="MIICTAGHVDHGKTSLLQALTGTNTAHLPEEQKRGLTIDLGYAY
                     LPIENDILGFIDVPGHQRFLSNMLAGLGGIQHALLVISAEEGIKPQTEEHLDILHLLN
                     FQHIMIVITKADRAEETQISHLIEQVKTSYPFLAEAKTFVTSAKTQQGIEELKAHLIQ
                     LNQQHTQTHKPFRYAIDRVFNVKGAGLVVTGTTVAGKVSVGSELFLSNGKKVRVKAIH
                     AQNTPSETGVAGQRLALNIANVEKEEIKRGDWITELEPKFATDRITVQLTANQAFKEN
                     SIVHLYHFASHITGKLNLLEGQQAVKNQQFFAEVILDEPLHIAVGDKLIIRSGDDSLT
                     LAGAEVLEIHSPKRHKRTEARLVLVKNLAKTTACNDFATRLDLYLQNRAMDLSLLLWA
                     EQCFAEDIAKLGFDVSSKWLFNSDFKAQAQQRVLEKMAEYHLQHQDQIGVTKARLYRI
                     ALLDLPENLANQFIEDLVEQKALVNSRGWLHLPEHRIELNADELQIWQQIRPLFEATN
                     QALWVRDIASQLSIDETQMRNLLYKVGKLGYLIPIVKDRFLLSEQIGQFATLIKDFIA
                     QNGSISVNQLRDE"
     gene            complement(1749..2045)
                     /locus_tag="PWX02_002118"
     CDS             complement(1749..2045)
                     /locus_tag="PWX02_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL1730089.1"
                     /translation="MMKFDYNIVWLQKAEIKLFYQADYILQQSQNPIIAEQFYDAIKS
                     EVDKLSFTADVYRLRKKKEIPILNGKYQVKFLIGQENVYIVDFKSARQNSYSFL"
     gene            complement(2042..2218)
                     /locus_tag="PWX02_002119"
     CDS             complement(2042..2218)
                     /locus_tag="PWX02_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730090.1"
                     /translation="MELILAQDLAKLRTDKPQSFYTNDELDLLHQLENGLIETKSHAQ
                     VMEDLRKKLGITEK"
     gene            complement(2287..2919)
                     /gene="pdxH"
                     /locus_tag="PWX02_002120"
     CDS             complement(2287..2919)
                     /gene="pdxH"
                     /locus_tag="PWX02_002120"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005575912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HDL1730091.1"
                     /translation="MDLHHIREDYTKKVLSIRDCHSNPITQFEQWLSESINAKVNEPT
                     AMNIATVAANGRPSSRMVLLKEVNSQGFVFFTNYQSRKGREIADNPFGCLTFFWAELQ
                     RQVRIEGKIAKIPEAESDAYFDSRPYTSRLGAWASEQSQPIENYKALLLKSAQFALKY
                     PLSVPRPPHWGGYILIPDSIELWQGRSSRLHDRIHYWLENNEWKKERLSP"
     gene            complement(2977..4359)
                     /locus_tag="PWX02_002121"
     CDS             complement(2977..4359)
                     /locus_tag="PWX02_002121"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDL1730092.1"
                     /translation="MQQLFRQIPSLDLLLKKPQAVELCQKFAYSSVAEQARSLLTQAR
                     QFISQQQALPDFIADENLFFIRLEQKVQALSEVKIRSVFNLTGTVLHTNLGRGLWSEK
                     AIQAATNAMRNNVALEFDIEAGKRSHRDNYISELLQILTGAEAACIVNNNAAAVLLML
                     ATFAQGKEVIVSRGELVEIGGAFRIPDIMAQAGCKLVEVGTTNRTHLKDYRNAITENT
                     AFLMKVHTSNYHIEGFTSSVSEEELVALGKEFNLPVITDLGSGSLTDMQALNLPAEPK
                     VQQKVAAGVDLVSFSGDKLLGGPQAGIIVGSQAMISQLQQHPLKRVLRCDKVILSALE
                     ATLRHYLFPEQLTDNLPTLHLLTQPLETLQHKAERLKQALHKRLNSAYILQIEPSLAQ
                     IGSGALPTETLPSVAVTISAEKQSDLIALEQQFKTYPSPIIGRFAQQKFWLDLRSVVE
                     FDKLIEMLEY"
     gene            4427..4521
                     /locus_tag="PWX02_002122"
     tRNA            4427..4521
                     /locus_tag="PWX02_002122"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4461..4463,aa:Sec,seq:tca)
BASE COUNT         1217 a         1041 c          937 g         1395 t
ORIGIN      
        1 aactcatctc gcaactggtt caccgaaatg ctgccgtttt gggcgataaa atctttaatt
       61 aacgttgcaa attgcccgat ttgttcgctt aataaaaatc tatctttgac gattgggatt
      121 aaatagccca atttcccgac tttatacagc aaattccgca tttgggtttc atcaatcgag
      181 agctggcttg caatatccct cacccaaaga gcttgattgg ttgcctcaaa cagcggtcga
      241 atttgttgcc aaatttgcaa ttcatcggcg ttcaattcga ttcgatgttc cggcaaatgt
      301 aaccaaccac gactattgac caaggctttt tgctccacca aatcctcaat aaattgattt
      361 gccaagtttt caggcaaatc caataacgct atgcgatata aacgagcttt ggtcacgccg
      421 atttgatctt gatgttgcag gtggtattct gccatttttt ccaacacccg ttgctgggct
      481 tgggctttaa aatccgaatt aaataaccat ttagacgaga catcaaaccc cagttttgca
      541 atatcctctg caaaacattg ctccgcccat aacagcaagg ataaatccat cgctctgttt
      601 tgcagataga gatcaaggcg tgtcgcaaag tcattacaag cggtggtttt tgctaaattt
      661 tttaccaaaa ctaaacgggc ttcggtacgt ttatgccgtt ttggtgagtg aatttccagc
      721 acctccgctc cggctaacgt taggctgtca tcaccgctgc ggatgatcaa cttatcacca
      781 acggcaatat gaagtggttc atctaaaatc acttctgcaa aaaattgctg atttttgacc
      841 gcttgttgcc cctcaagcaa attcaatttg ccggtaatgt gcgaagcaaa atggtaaaga
      901 tgcacaatgc tgttttcttt gaaggcttga ttggcagtga gttgaaccgt aatgcgatcg
      961 gtggcaaatt tcggctcaag ctcggtaatc caatcgcctc gcttgatttc ctctttttcc
     1021 acgttagcga tatttaacgc caaacgttgc cctgccactc ccgtttccga aggcgtattt
     1081 tgggcgtgaa tcgctttgac ccgcactttt ttaccattcg acaaaaataa ctcagagcca
     1141 acgctcactt ttcccgccac cgttgtgccg gtaacgacca accctgctcc tttcacatta
     1201 aacacacggt caatcgcata acggaacggt ttatgggttt gggtatgttg ttgatttaat
     1261 tgaattaaat gtgcttttaa ttcttcaatc ccttgctgcg tttttgccga agtaacaaag
     1321 gtttttgctt ccgctaaaaa cggatagctt gttttcactt gctcaattaa gtggctgatt
     1381 tgagtttctt ccgcccgatc tgccttagta atcacaatca taatatgctg gaaattcagc
     1441 aggtggagaa tatccaaatg ctcttcggtt tgtggcttaa tcccttcttc ggcagaaatc
     1501 accaacaggg cgtgttgaat gccgccaagc cctgccagca tattagataa aaaacgctga
     1561 tgacccggca catcaataaa ccctaaaata tcgttctcaa tcggtaaata ggcatagccc
     1621 aaatcaatgg ttagcccacg tttttgttct tcgggcaaat gggcggtgtt agtgccggtt
     1681 aaggcttgta aaagcgatgt tttgccgtgg tcaacgtgac cggcggtgca gatgatcatt
     1741 gatagtcctt ataaaaatga gtaactattc tggcgagcag atttaaaatc tacaatatag
     1801 acattttcct gaccaatcag gaactttact tggtacttac cgtttaaaat gggaatttct
     1861 tttttcttgc gaagtcgata aacatcggca gtaaaggaaa gtttatccac ttcacttttt
     1921 attgcatcat aaaattgttc tgcaataatc gggttttggg attgttgtaa tatataatct
     1981 gcttgataaa acaattttat ttctgctttc tgtagccaaa caatattgta atcaaacttc
     2041 atcatttttc cgtgattccc aattttttgc gtaaatcttc catcacctga gcgtgggatt
     2101 tagtttcaat taagccattt tctaattgat gtaataaatc aagttcatca ttagtataaa
     2161 aactttgtgg cttatctgtg cgtaattttg ctaaatcttg tgcgagtatg agttccatat
     2221 ttccccctta caaacttaga ttattcaata ttgtaacctg ataaaactaa agttcaagga
     2281 gtgtatttaa ggcgataacc gctctttttt ccactcatta ttttccaacc agtaatgaat
     2341 gcggtcgtgt agccggctac ttctgccttg ccagagttca atactgtcgg gaatcagaat
     2401 atagccaccc caatgcggtg ggcgaggcac tgaaagtgga tatttcaatg caaattgagc
     2461 tgattttagc aacaaagcct tgtagttttc gataggctga ctctgctcgc tcgcccaagc
     2521 ccccaaacgg ctggtgtaag gacgagagtc aaaataggca tcggattctg cctctggaat
     2581 ttttgctatt ttcccttcaa tccgcacttg acgttgtaat tctgcccaaa agaaggttaa
     2641 acatccaaaa ggattatcgg caatttcccg ccctttacgg ctttgataat tggtaaagaa
     2701 cacaaagcct tgtgaattta cctctttcaa cagcaccatt cggctacttg gtcgcccgtt
     2761 tgctgcaacg gtagcaatat tcattgctgt cggctcatta actttggcat tgattgactc
     2821 actcaaccat tgttcaaatt gggtgattgg gtttgagtga caatctcgga ttgaaagcac
     2881 ttttttggtg taatcttctc gaatgtggtg cagatccata aggattcctt tatcttattc
     2941 taaataatcg gacaggcaca gtctgcccta ccaaaattaa tattccaaca tctcaatcaa
     3001 cttatcaaac tccacgaccg agcgtaaatc taaccaaaat ttctgctgag caaaacgccc
     3061 gataatcggg cttgggtagg ttttaaattg ttgttccaat gcaattaaat cgctctgttt
     3121 ttcagcggaa atagtcactg ccaccgaagg taaggtttct gtgggtaacg caccgctgcc
     3181 gatttgagcg aggcttggct caatttgcaa aatataagca gaatttaacc gcttgtgtag
     3241 tgcctgtttt aatcgctctg ctttgtgttg caaggtttca agcggttgag tgagtaaatg
     3301 aagagtcggc aagttatcgg ttaattgctc cggaaataag tagtggcgta gcgttgcctc
     3361 aagtgccgat aaaatcactt tatcgcaacg tagcacccgt tttagcgggt gttgttgcag
     3421 ttggctaatc atcgcttggc taccgacaat aatgccggct tgcggcccgc ctaataattt
     3481 atcgcccgag aaggaaacca aatctacccc agccgctact ttttgctgaa cctttggctc
     3541 agcaggcaaa ttaagagctt gcatatcagt tagcgaacca ctgcctaagt cggtaatcac
     3601 aggcaggtta aactctttac cgagagccac caattcctct tccgacactg aactggtgaa
     3661 cccctcaatg tggtagttac tggtgtgaac tttcattaaa aaggcggtat tttcggtgat
     3721 cgcgttgcga taatccttta aatgagtgcg attagtcgta ccaacttcca ccaatttgca
     3781 accggcttga gccataatat ccggaatgcg gaatgccccg ccaatttcca ccagctcgcc
     3841 acgggaaacg atgacttcct tgccttgtgc gaaggttgct aacatcaaca acaccgctgc
     3901 cgcattatta ttcacaatgc aagcggcctc cgctccggta agtatttgca acaactcgga
     3961 aatgtagtta tctcggtgcg agcgcttgcc tgcttcaata tcaaattcca acgccacatt
     4021 gtttcgcatt gcgttggttg ctgcctgtat cgccttttcc gaccacaacc cacgccctaa
     4081 gttggtgtgt aacaccgtac cggttaagtt gaacacggaa cggattttca cctcactcaa
     4141 cgcttgcact ttttgttcca gccttataaa aaacaggttt tcatcggcaa taaaatccgg
     4201 caaggcttgc tgttgggaaa taaattgacg tgcttgagta agcaaactcc tagcttgttc
     4261 agccacgctg gaatatgcaa atttttggca taattcgacc gcttgtggct ttttcaacaa
     4321 gaggtcgagc gagggaattt ggcgaaatag ttgttgcata atgtgaatga ttgagagaat
     4381 attttggaca attctaataa ataaagtaca atattgccaa tttttcggaa gtgcgtcatc
     4441 tccggtgagg tggcaggact tcaaatcctg ttgaggacgc cagcgttctc gggtgggttc
     4501 aactcccatg cacttccgcc acatttcaaa agccgttaat ctttaacggc tttttgcatt
     4561 tctgccacat tctctctttc cgttttgata
//
LOCUS       DANJWC010000167         4566 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.167, whole genome shotgun sequence.
ACCESSION   DANJWC010000167 DANJWC010000000
VERSION     DANJWC010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.167"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            509..1861
                     /gene="lysC"
                     /locus_tag="PWX02_002123"
     CDS             509..1861
                     /gene="lysC"
                     /locus_tag="PWX02_002123"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HDL1730093.1"
                     /translation="MSHLSVAKFGGTSVANFDAMTSSANIVIADANTRVVVLSASAGV
                     TNYLVELANGCEKEHRDEVIAAVRQIQYNIIEKLQHQDAVKAEIDELLERIETLAESA
                     SLATSAALSDELISHGEMMSTKIFTQLLIERGHPAVWVDVRDVVATNSHYGKAAPNDE
                     KTQQQSDNVIKPLIASGKIVITQGFIGRDDEGKTTTLGRGGSDYSAALLAEVLNANDV
                     LIWTDVPGIYTTDPRVVPNAQRIDTMAFNEAAEMATFGAKVLHPATLLPAVRSNIPVY
                     VGSSKAPEQGGTWVTRDPQPRPTFRAIALRRNQILLTLSSLSMLHAQGFLANVFAILA
                     KHKISVDVITTSEVSVALTLDKTGSASSGADMLSAELLDELNAYCNVQVESDLALVAI
                     IGNNLHAQSGVAKKLFHTLENYNIRLISYGASSNNVCTLVKNAEADDVVRSLHKGLFE
                     "
     gene            complement(1932..4178)
                     /gene="parC"
                     /locus_tag="PWX02_002124"
     CDS             complement(1932..4178)
                     /gene="parC"
                     /locus_tag="PWX02_002124"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDL1730094.1"
                     /translation="MTTEINYEGIEQMPIKRFTEDAYLNYSMYVIMDRALPFIGDGLK
                     PVQRRIIYAMSELGLNASAKYKKSARTVGDVLGKFHPHGDSACYEAMVLMAQPFSYRY
                     PLVDGQGNWGAPDDPKSFAAMRYTESKLSKIAEILLGELGQGTVDYQPNFDGSLEEPK
                     YLPARLPHILLNGTMGIAVGMATDIPPHNINELADASVMLLDNPKATLDDVLSVVQGP
                     DYPTEAEIITPKAEIARMYEQGRGSIKMRAVWKKEEGEIVISALPHQASPSKIIEQVA
                     TQMRNKKLPMVDDIRDESDHENPIRIVIVPRSNRIDFDALMDHLFATTDLEKSYRVNM
                     NMIGLDGKPAVKNLLTILNEWLSFRRTTVTRRLNYRLDKILNRLYILDGLMIAFLNID
                     EVIEIIRNEDEPKAELMARFNLTDVQAEAILNLRLRHLAKLEEHQLQAEKSELEKERD
                     ELQLILGSERRLNSLIKKEIQADAKAFASPRRSPLVERAESKAIAESDLTPIEDVTVI
                     LSEKGWVRCAKGHDIDVQALSYRAGDDYLAHARGRSNQPVVFLDSTGRAYALDPTSLP
                     SARSQGEPLTGKITLPEGATVQQVLMANSETKVLMASDSGYGFICTFEDLVSRNKAGK
                     AVISLTENAKVLPPQLLENDENLSLVAMSNVGRMLVFPVSELPALSKGKGNKIINISA
                     AAAKSGDEYLARLLVIKPTNSLVFVSGKRKITLKPSDIDNYRGERARKGSQLVRGLST
                     NSTVEIVE"
     gene            4363..>4566
                     /locus_tag="PWX02_002125"
     CDS             4363..>4566
                     /locus_tag="PWX02_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005756281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL1730095.1"
                     /translation="MAKRFKLLTVLGISFSLSVAANAQTITGAGASFPYPIYAKWAGL
                     YEKETGNKVNYQSIGSGGGQQQII"
BASE COUNT         1277 a         1026 c          912 g         1351 t
ORIGIN      
        1 ttaaagaaca atcaaataaa tttggttttg tgatccattt caaaatattt tttaggagtt
       61 tttattcaac attagaaata tgtttacttt ttatcgctat attttaaata attacctaaa
      121 tagcattgga ttacctaaat attcattgac aaaacattaa taaaacggta tttctatgca
      181 cacttttcaa tactgttgaa aaagagtaat gggcttgcca ttacgggaga ggtgcaacac
      241 tcaggcaact tgattttagg caactagggc cgtttgagaa ttaagtgatg ggagtgattg
      301 ccgaggtgtg aattcactag actggaatta acatcggctg aataggttga atcctatcag
      361 ctgtcattta ataggttatt tcaccatatt aaatggagtg ctctgattaa ttggttcaac
      421 aataaaaccg tataatctac tcaaatttcc tttccgaata tcccctaaca caatatttat
      481 ctatttttgt tttaaggaaa tttttattat gtctcatctt tctgtggcta aatttggtgg
      541 tacaagtgtt gcaaattttg atgcaatgac ctcttctgcc aatattgtaa ttgctgatgc
      601 aaatactcgt gtggtggtac tttctgcctc tgctggcgta accaattact tagtggaact
      661 tgctaacggc tgtgaaaaag aacaccgtga tgaagtcatc gcagcagtcc gccaaattca
      721 atacaatatt attgaaaaat tacaacatca agatgccgtt aaagcggaaa tcgacgaatt
      781 attagaacgt attgaaacat tagcagaatc agccagcctt gctacttcag ctgccttaag
      841 tgatgagtta atcagccatg gggaaatgat gtccaccaaa atcttcacgc aattattaat
      901 tgaacgtgga cacccggcag tatgggtaga tgtgcgtgac gttgtggcaa caaacagcca
      961 ttatggaaaa gcagcaccaa atgacgagaa aacccagcaa caatcagaca atgtgattaa
     1021 gccattaatc gcatcaggca aaattgtgat tacacagggc tttattggtc gtgatgatga
     1081 aggcaaaacc actactttag gacgtggcgg ttcagactac tctgctgcat tgttagcaga
     1141 agtattaaat gctaacgatg tactcatttg gacagacgta ccggggattt ataccaccga
     1201 tcctcgtgtt gtaccgaacg ctcaacgcat tgacacaatg gcatttaacg aagcggcaga
     1261 aatggcaaca tttggagcga aagtattaca cccagcaacc ttattaccgg cagtgcgtag
     1321 caatatccct gtatatgttg gctcaagcaa agcaccggaa caaggcggca cttgggtaac
     1381 ccgtgatcca caaccacgcc cgactttccg tgcgattgca ttacgccgca accaaatttt
     1441 actcactctt tccagccttt caatgctaca cgcacaaggt ttcttagcga atgtatttgc
     1501 gattttagcg aaacacaaaa tttcggttga tgtgattacc acttccgaag tcagcgttgc
     1561 attgacccta gataaaacgg gctcagcctc ttcaggtgca gatatgttat cagcggaatt
     1621 actcgatgag cttaacgcat attgcaatgt gcaagtagaa agcgacctcg ctttagtcgc
     1681 tattatcggc aacaacttac acgctcagtc cggtgtggcg aaaaaattat tccacactct
     1741 tgagaattac aacattcgcc taatcagcta tggagcaagc agcaataatg tttgtacctt
     1801 agtgaaaaat gcagaagcag acgatgtggt tcgttcgctt cacaaaggct tatttgaata
     1861 agcaataact ccattgcaag cggttatttg gggcaaaatt gttggattta ccccctaacc
     1921 gcttcttctc tttactcaac aatctctacc gttgaattgg tactaagccc tctcactagc
     1981 tgtgagcctt ttcttgctcg ttcgccacgg tagttatcaa tatcgctcgg ttttaatgtg
     2041 attttgcgtt taccggacac aaagaccagc gagttggttg gtttgatgac caataaacgg
     2101 gctaaatatt catcaccgga ttttgctgcg gcagcggaaa tattgataat tttattgcct
     2161 ttacctttgg aaagtgccgg tagttcactc accgggaaaa ccaacatcct accgacatta
     2221 ctcattgcca ctaaagataa attttcatca ttttccaata attgcggcgg cagcactttt
     2281 gcgttttcgg ttaaagaaat aaccgctttg ccggctttat tacgagaaac caaatcttca
     2341 aaggtgcaaa taaagccgta gcctgaatcg gatgccatta gcactttggt ttccgaattt
     2401 gccattaata cttgttgaac agttgcacct tcaggtagag tgattttgcc tgtaagtggc
     2461 tcaccttgcg agcgtgcaga aggcagtgaa gttggatcga gtgcataagc tcgcccagtg
     2521 ctgtctaaga acaccacagg ctgattactt ctgccccttg cgtgggcaag ataatcatct
     2581 ccggctcgat agctgagggc ttgaacatca atatcatgtc ctttggcaca acgtacccag
     2641 cctttttcag acaaaatcac tgtcacatct tcgattgggg ttaagtcgct ttccgcaatc
     2701 gctttagact ccgctcgttc caccagaggt gagcggcgtg ggctggcaaa tgctttggca
     2761 tcggcttgaa tctctttctt aatcaggctg ttcaaacggc gttccgagcc taaaatcagt
     2821 tgcaattcat cacgttcttt ttccagttcg gatttttccg cctgtaattg atgctcttcc
     2881 aatttggcta agtggcgtaa acgtaagttc aggatcgcct ctgcttgcac atcggtcaga
     2941 ttaaaacgag ccattaactc ggctttcggc tcatcttcat tgcgaataat ctcaatcact
     3001 tcatcaatat tgagaaatgc aatcatcaaa ccgtctaaaa tatacaagcg gtttaaaatt
     3061 ttatctaatc ggtaattcag gcggcgagtg acagtggtac gtctaaaact cagccactca
     3121 ttgagaatag tcagcaaatt cttcactgcc ggcttgccgt ctaagccaat catattcata
     3181 ttgactcggt agcttttctc caaatcggtc gtcgcaaaca ggtgatccat taacgcatca
     3241 aaatcaatgc gattagagcg tggcacaatc acaatgcgaa tcgggttctc gtggtcagat
     3301 tcatcacgaa tatcgtccac catcggtagt tttttattcc gcatttgggt tgctacttgc
     3361 tcaataattt ttgaaggaga ggcttggtgc ggcaaggctg aaatcacaat ttcaccctct
     3421 tcttttttcc acaccgcacg cattttaata gaaccacgcc cctgctcata cattctggca
     3481 atttccgctt ttggtgtgat gatttcagcc tctgtcgggt aatccggccc ttgtaccacg
     3541 cttagtacat catcaagcgt tgctttcggg ttatccagca acatcacact ggcatcagcc
     3601 aattcattaa tattgtgtgg tggaatatcg gttgccattc ccaccgcaat ccccatcgtg
     3661 ccattcaaca aaatatgcgg taaacgagcc ggcagatatt tcggttcttc caacgagcca
     3721 tcaaaattgg gctgataatc caccgttcct tgccctaact cgcccaataa aatttctgca
     3781 attttagaca gtttcgattc ggtataacgc attgcagcga aggatttcgg atcgtccgga
     3841 gccccccagt tgccttgtcc gtccactaac ggatagcggt aagagaacgg ttgagccatt
     3901 agcaccattg cttcatagca ggcactatca ccgtgcggat ggaatttacc taatacatcc
     3961 cccacggtac gggctgattt tttatacttt gccgaggcgt ttagcccaag ttccgacatc
     4021 gcataaataa tacggcgttg caccggtttc aagccatcgc caataaacgg caatgcccgg
     4081 tccataatta catacatcga ataattgagg taagcatctt cggtaaaacg cttaatcggc
     4141 atctgttcaa tgccttcata attgatttct gtggtcattt tttatcgata tttatattaa
     4201 aaaaacgagg gctattttct acgatttagc ctgtttagtc tagcctgaaa cacaagcggt
     4261 cgttttttct gattttttta ccattgtcat cattttgtca tattcttttt ctacactaag
     4321 cacgtgaaat taactcacgc taactttaag gagaacaata tgattgccaa acgatttaaa
     4381 ctacttaccg ttttaggcat ttctttttca ctttctgtcg cagcaaatgc tcaaaccatc
     4441 acaggagccg gtgcttcttt cccttatcct atctacgcca aatgggcagg tttatatgaa
     4501 aaagagaccg gtaataaagt caattaccaa tctatcggtt ccggtggcgg tcagcaacaa
     4561 attatt
//
LOCUS       DANJWC010000168         4533 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.168, whole genome shotgun sequence.
ACCESSION   DANJWC010000168 DANJWC010000000
VERSION     DANJWC010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.168"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..762)
                     /locus_tag="PWX02_002126"
     CDS             complement(<1..762)
                     /locus_tag="PWX02_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDL1730096.1"
                     /translation="MKYDLHAHSTASDGVLSPTELVQRAVEQGVTMLALTDHDTISGI
                     QEAKNFAQNQPLELISGVEISILWQEKSIHLAALNIDENNEELVKFLDNQAQLREERA
                     VEIGEKLAKVGIANAYEGAKALATGEVTRAHYGRFLYEQGYVRNIEHAFKRYLGMGKP
                     AYVKPRWSSLEDAVNVTHTSGGVISIAHPLRYKMTARWIRRLIADFKQAGGDGIEVSG
                     CGQTPDQRQLLARWAKEFDLYGSVGSDFHYPTGWIE"
     gene            complement(774..1373)
                     /locus_tag="PWX02_002127"
     CDS             complement(774..1373)
                     /locus_tag="PWX02_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HDL1730097.1"
                     /translation="MTMDNLPLEQHPFDPILPEQATVVMVGTFPPTSEKRCMEFHYPN
                     FQNDMWRIMGNIFYADPNYFRVGEEKRFDPIRIEEFLREKGIALCSSAKTAIRLKGNA
                     SDKDLKIVDPIDMDELLLKLPKLKWLFTTGGLATDTLLGLLPEKHKAPKTNEWVVYPY
                     SATRELYLYRLPSTSRAYPLSLANKTEAYRQFFRTAGLI"
     gene            complement(1370..2917)
                     /gene="sbcB"
                     /locus_tag="PWX02_002128"
     CDS             complement(1370..2917)
                     /gene="sbcB"
                     /locus_tag="PWX02_002128"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HDL1730098.1"
                     /translation="MSDKFSFFFYDYESFGVSPALDRPAQFAGIRTDADFNIIGEPVM
                     FFCKQTPDYLPSPEAVMVTGITPQQCNAEGVSEPEFAARIHAEFSRANTCTIGYNNIR
                     YDDEMTRYTFFRNFYDPYEYSWKNGNSRWDLLDLVRACYALRPDGIEWPTDETGMPSF
                     KLENLTKANGIAHENAHDAMSDVYATIAMAKLIKQKQPKLFDFFFNLRSKKEVEKLID
                     TGEMTPLVHVSGMLGNYRGNTAWVVPLAWHSTNQNAVIVCDLSGDMNGLLTENAEVLR
                     QRLYTKRDELAENELSVPLKLVHINKCPILAPAKTLLPENAERLGIDRALCLENLKTL
                     KAQKSLIREKVIEIFNEERTFEPSTNVETTLYDGFFSPADKNNMAILRTLPPEDLANH
                     GLKFEDPRVEALLFHYRARHYPQTLSRAEQIKWQKHCNQQIEAKAEQFAQSIDDLFQQ
                     HHDNPEKVKLLENLTAYAEQISQQQAVIYRQNVAKDEKLLSELNRVAEQPLDKTEKLK
                     MLKELIK"
     gene            3320..>4533
                     /gene="asnS"
                     /locus_tag="PWX02_002129"
     CDS             3320..>4533
                     /gene="asnS"
                     /locus_tag="PWX02_002129"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDL1730099.1"
                     /translation="MSKFPTVSEILSGKIAVGEEATVRGWVRTRRDSKAGLSFLSVYD
                     GSCFDPIQAIINNDLPNYNAEVLRLTAGCSVVVTGKVVESPAEGQAVELQASKVEVLG
                     WVEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHCLAQAIHRFFHEQGFYW
                     VATPVITASDTEGAGEMFRVSTLDLENLPRGENGKVDFNQDFFGKESFLTVSGQLNAE
                     TYACALSKVYTFGPTFRAENSNTTRHLAEFWMMEPEVAFADLNDNAKLAEDMLKYVFK
                     AVLAERKDDMEFFVKHVDKEAISRLENFINSPFAQVDYTDAIEILLKSGKEFEFPVSW
                     GIDLSSEHERFLAEEHFKSPVVVKSYPKDIKAFYMRLNDDGKTVAAMDVLAPGIGEII
                     GGSQREERLEVL"
BASE COUNT         1278 a          975 c          935 g         1345 t
ORIGIN      
        1 ctcaatccag ccagttgggt agtggaaatc agaaccgacc gagccatata aatcaaactc
       61 ttttgcccaa cgggcaagta attgccgttg atcaggtgtt tgtccgcagc ctgatacttc
      121 aatgccatcg cctccggctt gtttgaaatc tgcgattaaa cggcgaatcc aacgagcagt
      181 cattttatag cgtagcgggt gagcaatcga aatcacaccg cctgaggtgt gggtaacatt
      241 caccgcgtct tctaaactac tccatctcgg tttcacatag gcaggtttcc ccattcccag
      301 ataacgctta aaggcgtgtt caatattgcg gacatagcct tgttcataca aaaaacgccc
      361 ataatgtgcc cgagttactt cccctgttgc caacgctttt gccccctcat aggcatttgc
      421 aatcccgact tttgctaatt tttcaccaat ttcgaccgct ctctcttccc ttaattgggc
      481 ttggttatcc aaaaacttca ccagctcctc gttgttttca tcaatattca acgccgcaag
      541 atgaatactt ttttcttgcc ataaaatcga aatttccacc ccagagataa gctcaagcgg
      601 ttgattttgg gcaaaatttt tcgcttcttg aatgcccgaa atcgtatcgt gatcagttaa
      661 agctagcatt gttacccctt gctctaccgc acgttgtacc aattcagtgg gagaaagcac
      721 gccatcagag gcggtactgt gggcgtgtaa atcatatttc atgttattta tccttaaatt
      781 aagccggcag tcctgaaaaa ttggcgatag gcttcggttt tgttggctaa gctgagtgga
      841 taagccctcg acgtcgaagg caggcgatat aaataaagtt ctcttgtggc ggaataagga
      901 tagaccaccc attcgttggt tttcggagct ttgtgttttt ccggcaataa accgagcaac
      961 gtatcggttg ccaaaccacc tgtggtaaat agccatttca gtttgggtaa tttgagcaac
     1021 agttcgtcca tatcgatagg atcaacaatt tttaaatctt tatctgaagc gtttcctttt
     1081 aaacgaatcg ctgtttttgc cgaagaacag agcgcaatgc ccttttctcg caaaaactcc
     1141 tcaatccgaa tcggatcaaa gcgtttttct tcaccgactc gaaaatagtt agggtcagca
     1201 taaaaaatat tgcccataat tcgccacata tcattttgaa aattcgggta atgaaattcc
     1261 atacaacgct tttccgatgt aggtggaaaa gtgccgacca ttaccaccgt tgcttgttca
     1321 ggtaaaatag ggtcaaacgg gtgctgttct agcggtaaat tatccattgt catttaatca
     1381 actcttttaa catcttgagc ttttcggttt tatctagggg ctgttccgct actctgttaa
     1441 gctcgctcag caatttttca tcttttgcaa cattttgacg ataaatgacc gcttgttgtt
     1501 gcgaaatctg ctcggcgtaa gcggtcaaat tttctaataa ttttaccttt tccgggttgt
     1561 cgtgatgttg ttggaacaag tcgtcaatcg attgggcgaa ctgctctgct tttgcctcaa
     1621 tttgctggtt gcaatgtttc tgccatttga tctgctcggc tcgactgagt gtttgtggat
     1681 agtgccttgc tcggtaatgg aatagtaacg cctctactcg agggtcttca aatttcaagc
     1741 cgtggttggc taaatcttca ggcggtagcg tgcgtaaaat cgccatatta tttttatccg
     1801 caggcgagaa aaagccgtca tacaaagtgg tttctacatt ggtcgaaggc tcaaacgtgc
     1861 gttcttcatt aaaaatttca atcacttttt cccgaatcag cgatttctgt gcttttaaag
     1921 ttttcaaatt ttccaaacat aaagctctgt ctatgcctaa acgttctgca ttttcaggca
     1981 gtaaggtttt tgccggtgct aaaatcgggc atttattaat atgcactaat tttaacggca
     2041 cagataactc attttcggct aattcatctc gtttggtgta taaacgctga cgtaaaacct
     2101 ctgcattttc agtcaataag ccattcatat cgcccgataa atcacacaca attacggcat
     2161 tttgattagt tgagtgccaa gccagtggca caacccaagc ggtatttcct cgatagttgc
     2221 ccagcatacc ggaaacgtgc acaagcggtg tcatttcacc agtgtcaatt aacttctcta
     2281 cttctttttt actgcgtaaa ttaaagaaaa aatcgaacaa ctttggctgt ttttgcttaa
     2341 ttaattttgc cattgcgatg gtggcataca catcagacat cgcatcgtgt gcattttcat
     2401 gggcaatgcc attggctttg gtgaggtttt ccaatttaaa acttggcata ccggtttcat
     2461 ctgttggcca ttcaatacca tcaggtctta acgcataaca ggcacggact aaatctaaca
     2521 agtcccaacg agaatttccg tttttccagc tatattcgta aggatcgtaa aaattgcgga
     2581 agaaggtata acgggtcatc tcatcatcat aacgaatatt gttataaccg atagtacaag
     2641 tattcgctcg gctaaattca gcgtgaattc gggctgcaaa ttccggctca gatacacctt
     2701 cagcgttaca ttgttgcggt gtaatcccgg taaccatcac cgcttcgggg ctgggtaaat
     2761 aatccggcgt ttgcttacaa aaaaacatta ccggttcacc gataatatta aaatccgcat
     2821 cggttcgaat accggcaaat tgtgccggac gatctaacgc cggactcacg ccgaaacttt
     2881 cataatcgta aaagaaaaaa gagaatttat cgctcattcg ttcaatttgt tagaaaaaat
     2941 tgggctgatt gtagcacagt aaggcagaaa ggagggaata caagcggtca atttccgtta
     3001 gaaatttgca aatgctaaaa taaaaaaaag cccctaatca aaaaaagcga aaaggggcgg
     3061 aggtcttacc taaggaaata tgaaaaaaac ttgcataaat ctgttacata tactcagact
     3121 gcctaactga acgaaagttc attaaaaaat aaaaaatttt ttaacaccaa gcaaaatcag
     3181 atttaactac ttgcaatgct tatatagact cacaaatttg caaataattc cgtaaaatta
     3241 ttatatttcc ccctattttc ttgtagaata cggaacgttt cttatattaa aactcacaat
     3301 aaagaatttg aggtattcaa tgtctaaatt tccaacagtt tcggaaatcc tatccggcaa
     3361 gatagcggta ggtgaagaag cgacagtgcg tggttgggtg cgtacccgcc gtgattcaaa
     3421 agccggttta tctttcttga gcgtttacga tggttcttgc tttgacccaa ttcaagcgat
     3481 tattaataac gatctaccaa attataatgc cgaagtatta cgtttaaccg caggttgctc
     3541 tgtggtggtg accggtaaag ttgtggaatc ccctgctgaa ggtcaagccg ttgaattaca
     3601 agcaagtaaa gtggaagtgc ttggttgggt ggaagatcct gatacctacc caatggcagc
     3661 gaaacgccac tctatcgaat atctgcgtga agtcgctcac ttacgccctc gtacgaactt
     3721 aatcggtgca gttgctcgtg ttcgtcactg tttagctcaa gctatccacc gtttcttcca
     3781 cgagcaaggt ttctattggg tagcaacacc tgtaattacc gcctcagata ctgaaggtgc
     3841 aggtgaaatg ttccgtgttt ctacgctgga tttagaaaat ctacctcgtg gcgaaaacgg
     3901 caaagtggat tttaaccaag atttcttcgg taaagaatcg ttcttaaccg tatccggcca
     3961 gttaaatgcg gaaacctatg cgtgtgcctt aagtaaagtt tatactttcg gtccgacatt
     4021 ccgtgcagaa aactcaaaca caactcgcca tcttgcagaa ttctggatga tggagccgga
     4081 agttgccttt gccgacttaa acgacaatgc aaaattagcc gaagatatgc tgaaatatgt
     4141 gtttaaagcg gtgcttgcag aacgtaaaga tgatatggaa tttttcgtta aacacgttga
     4201 taaagaagcg attagtcgtt tagaaaactt tatcaattcg ccatttgctc aagtagatta
     4261 caccgatgcg attgagatct tattgaaatc cggcaaagaa tttgaattcc cggtgtcttg
     4321 gggaatcgat ctttcctctg aacacgagcg tttcttagcc gaagaacact ttaaatcgcc
     4381 ggtggtcgtg aaaagctatc caaaagacat taaagccttc tatatgcgtt taaatgatga
     4441 cggcaaaacg gttgccgcaa tggacgtttt agctccggga atcggtgaaa ttatcggtgg
     4501 ttctcaacgt gaagaacgtt tagaagtgtt aga
//
LOCUS       DANJWC010000169         4497 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.169, whole genome shotgun sequence.
ACCESSION   DANJWC010000169 DANJWC010000000
VERSION     DANJWC010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.169"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..500)
                     /gene="mdh"
                     /locus_tag="PWX02_002130"
     CDS             complement(<1..500)
                     /gene="mdh"
                     /locus_tag="PWX02_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDL1730100.1"
                     /translation="MCQSKKVALLGAAGGIGQSLALLLKLNLPAKSELSLYDISPVTP
                     GIAVDLSHIPTDVKVTGFAGEDPTEALKDADVVVISAGVARKPGMTRADLFNTNATIV
                     HNLVEKAAKVCPKACIAIITNPVNTIIPIAAEVLKKAGVYDKNKLFGVTTLDVIRANT
                     FVAEAK"
     gene            696..1157
                     /gene="argR"
                     /locus_tag="PWX02_002131"
     CDS             696..1157
                     /gene="argR"
                     /locus_tag="PWX02_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDL1730101.1"
                     /translation="MDKIDSLSEAFKSLLKEEKFGSQSEIVAALQEMGFDNINQSKVS
                     RMLSKFGAVRTRNPKMEMVYQLPVELSVPTTSSPLKNLVVDIDHNDALIVVKTSPGAA
                     QLIARLLDSMGKAEGILGTIAGDDTIFITPVRNMQIEKLVKNVTQLFETSL"
     gene            1207..2103
                     /locus_tag="PWX02_002132"
     CDS             1207..2103
                     /locus_tag="PWX02_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDL1730102.1"
                     /translation="MHIFITGGTGFIGRELIQQLKSENHHFTLLTRDTEAKSGDLQIN
                     YCYSLDELVDLNDFDAVINLAGEPIFDKRWTDEQKQKLIESRVNLTNKLSQLMNASSN
                     PPHTFLSGSAIGIYGDLPPSANFYTESTACYQDCFTNRLCQQWEAAALKAASDKTRVC
                     LIRTGTVFSPEGGALKKILPLYRCGLGGKLGSGKQYWAWISRRDHVQAVIFLLKNANC
                     SGAYNFVAPNPVRNEEFNRILANAVNRPAFFTVPEFALKLGLGERACLVLDNQQLIPE
                     KLLAASFVFQDPILTENVFLNP"
     gene            2148..4346
                     /gene="uvrD"
                     /locus_tag="PWX02_002133"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             2148..4346
                     /gene="uvrD"
                     /locus_tag="PWX02_002133"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HDL1730103.1"
                     /translation="MDFSLLLDGLNDKQREAVAAPIGNYLVLAGAGSGKTRVLTHRIA
                     WLIGVENVPESNILAVTFTNKAAAEMRHRIEYTLSQSGDNRLFGMWVGTFHSIANRLL
                     RSHYLDADLPQDFQIMDTEDQQRLLKRLLKLHNIDEKHFPPKHVAWYINAQKDKGKRA
                     KDIDHHNDPNEKKLVEIYQIYQDACDRAGLLDFAELLIRAYELFKTKPTILQRYQQRF
                     QQILIDEFQDTNNIQYDLIRLLAGDVGKVMIVGDDDQSIYGWRGAQVENIQRFLNDYD
                     NAQTIRLEQNYRSTGNILSCANELIANNDNRLGKNLWTDSGDGEPVDIYCAFNELDEA
                     RFVASQIKQWKEDEGDLAECAVLYRSNSQSRVIEEALIQANIPYRIYGGMRFFERQEI
                     KDALAYLRLIANRQDDAAFERVINTPTRGIGDRTLDTLRQITRGRQITLWQAIQVAVQ
                     EEQLSGRATSSLLRFVELINALDQETEQMSLAEQTDFVIKKSGLYEMYKQEKGEKGEV
                     RIENLEELVTATKQFVKPDEAEELTDLTAFLTHASLEAGEAQASQHEDYVELMTLHSA
                     KGLEFPRVFIVGVEEGIFPSGMSFDEGRLQEERRLAYVGITRAKKKLTISYAESRRLY
                     GKEERHVPSRFIAELPEEHIREVRLRGSINRAASFNQANSFAKNPTKTTASFLNDDTW
                     KMGQKVNHAKFGQGTIINVEGSGEATRLQVAFVGNGIKWLIAKMANLERI"
BASE COUNT         1375 a          851 c          994 g         1277 t
ORIGIN      
        1 tctttcgctt cagccacaaa ggtattcgct cgaataacat ctaatgttgt aacaccgaaa
       61 agtttatttt tgtcatacac accggctttt ttcaatactt ctgccgcaat cggaataatg
      121 gtgtttaccg ggttagtaat aattgcaatg caagctttag ggcaaacttt cgctgctttt
      181 tcgactaagt tatgcacgat agtggcattc gtattgaata aatcggcacg tgtcatcccc
      241 ggcttacggg caacaccagc agaaataacg accacatcgg catcttttag tgcctcagtc
      301 ggatcttcac ctgcaaaacc ggttactttt acatcagttg gaatatggct taaatccact
      361 gcaattcctg gagtaacagg ggaaatatcg taaagagata attcagattt agccggtaaa
      421 ttgagtttta ataataaggc gagtgattga ccgattccac ctgcagcacc taaaagggca
      481 acttttttac tttgacacat aaaatttctc ctaaatgtta tggaagttta gaccaattta
      541 gtctaagcca attttttggg cgattcaaac ggcaaatctc atttttgaga catagagcaa
      601 atttttagct gatttttatg ggttttattg atttatttgc ataaataagc aagaattatg
      661 aatattttta agtttttgtg aataagagtg tgaaagtgga caagatagat agtttatcag
      721 aagcgtttaa atcgctgtta aaggaagaaa aattcggctc gcaaagtgaa attgttgccg
      781 cactacaaga gatgggcttt gataatatca accaatccaa agtttctcga atgttatcaa
      841 aatttggtgc agtcagaacc cgtaatccta aaatggaaat ggtgtatcag ctaccagtcg
      901 aattaagtgt accgactacc tccagcccac ttaaaaattt ggtggtagat attgatcata
      961 atgatgcgtt aattgtggtc aaaaccagcc ccggtgcggc tcaactgatt gcacgtttgc
     1021 tcgattcaat gggtaaagcc gaaggtattt taggcactat tgctggcgat gatacgattt
     1081 ttataacgcc ggtacgcaat atgcaaattg aaaaattggt gaagaatgtg acacagttat
     1141 ttgaaacatc attatagaat aagtaaagga ggacttaatg ttctccttat tttttaggag
     1201 attattatgc acatttttat aacaggtggt acgggtttta ttgggcgtga attaatccag
     1261 caattaaaat ctgaaaatca ccactttaca cttcttacta gagatacaga agcgaaaagt
     1321 ggtgatttac agattaatta ttgctattca cttgatgaac ttgttgattt aaatgatttt
     1381 gatgcagtaa ttaaccttgc cggtgagccg atttttgata aacgttggac agacgaacaa
     1441 aaacagaaat tgattgaaag ccgggttaac ctaacgaata agctcagtca attaatgaat
     1501 gcaagctcta accctccgca tacttttctc tccggttcgg caatcgggat ttatggcgat
     1561 ttgcctcctt ctgcaaattt ttatacagaa tcgactgctt gttatcaaga ttgctttacc
     1621 aaccggcttt gccaacagtg ggaagccgct gcactaaaag ccgcaagtga taaaacccga
     1681 gtgtgtttaa ttcgtaccgg cacggtattt tcgcccgaag gcggagcatt aaagaaaatt
     1741 ttaccgcttt atcgatgcgg attaggcgga aaacttggtt caggcaaaca atattgggcg
     1801 tggatcagcc gccgggatca cgtacaagcg gtcatttttc tgctcaaaaa tgcaaattgt
     1861 tcgggtgctt ataattttgt tgcaccaaat ccggtgagaa atgaagaatt taatcgtatt
     1921 ttagcgaatg ctgttaatcg cccggcattc tttactgtgc ctgaatttgc cttgaaatta
     1981 ggcttaggcg aacgagcttg tttagtctta gataatcaac agctcattcc ggaaaaacta
     2041 ctcgcagcca gttttgtatt ccaagatccg attttaaccg agaacgtttt cttaaaccca
     2101 taatttaggc tataatgctg tataaaagca cagattaaaa ggtgattatg gatttctcat
     2161 tattattaga cggtttaaat gacaagcaga gggaggcggt tgctgccccg attggtaatt
     2221 atttagtgtt agccggggcg ggttcgggca aaactcgggt tttaacccat cgcattgcgt
     2281 ggctgattgg ggtggaaaat gtgccggaat ccaatatttt ggctgttacc tttaccaata
     2341 aagcggcggc ggaaatgcga catcggattg agtacactct ttcccaatcg ggcgataatc
     2401 gattgttcgg aatgtgggtt ggtactttcc acagcattgc caaccgctta cttcgttccc
     2461 actatttaga tgcggattta ccgcaagatt tccaaattat ggataccgaa gatcagcaac
     2521 gtttactcaa acgtttactg aaactgcata atattgatga gaaacatttt ccgccaaaac
     2581 acgttgcgtg gtatatcaac gcccaaaaag ataaaggcaa acgggcaaaa gatattgatc
     2641 accataacga tccgaatgaa aaaaaattgg tggagattta ccaaatctat caagatgcct
     2701 gcgaccgagc cggtttgtta gattttgccg agctgttgat tcgggcttat gaactattca
     2761 aaaccaaacc aaccattttg caacgttatc aacaacgttt tcagcaaatt ttgattgatg
     2821 agtttcaaga caccaacaat attcagtacg acttaattcg tttgcttgcc ggcgatgtcg
     2881 gcaaagtgat gattgtcggc gatgatgatc agtctattta tggttggcgt ggggcacagg
     2941 ttgagaatat ccaacgtttc ttaaatgatt atgacaacgc tcaaaccatt cgcttagagc
     3001 aaaattaccg ctctaccggc aacattttaa gctgtgccaa tgaattaatt gccaataacg
     3061 ataaccgctt aggcaaaaat ttatggacag atagcggtga tggcgagcct gtggatattt
     3121 actgtgcctt caacgaactt gatgaagctc gctttgttgc ctcgcaaatc aagcaatgga
     3181 aagaagacga gggtgatttg gcggaatgtg cggtgttgta tcgcagcaac agccaatctc
     3241 gtgtgattga ggaagcttta attcaagcga atattcctta tcgcatttac ggtggaatgc
     3301 gattctttga acgccaagaa attaaagatg ctctggctta tttacgctta atcgccaatc
     3361 gtcaagacga tgcggcgttt gaacgggtga tcaacacgcc aaccagaggc attggcgatc
     3421 gtaccttaga cacactccgt caaattaccc gtggtcgcca aattacgctg tggcaagcaa
     3481 ttcaagtggc ggtgcaagaa gaacaacttt cagggcgtgc cacttcttcc ttattgcgtt
     3541 ttgtagagct gattaatgct cttgatcaag aaaccgagca gatgtcgctt gccgaacaaa
     3601 ccgattttgt gattaaaaaa tcgggtttat atgaaatgta taagcaagaa aaaggcgaaa
     3661 agggcgaagt tcggattgaa aacttagaag agttagttac cgcaactaag cagtttgtga
     3721 aacctgacga agcggaagag ctgaccgatc ttaccgcatt tttaacccac gcctcacttg
     3781 aggctggaga agcccaagcc tcgcaacacg aagattatgt cgaactgatg acgctacatt
     3841 ccgccaaagg attggagttt ccccgtgtgt ttattgtggg cgtggaagaa gggattttcc
     3901 caagcggaat gagctttgat gaaggcaggt tgcaagaaga acgccgtttg gcttatgtcg
     3961 gcataacccg agctaagaaa aaactgacga ttagctatgc cgaatcaaga cggctttatg
     4021 gtaaagaaga acgccacgtt ccgtctcgct ttattgctga attacctgaa gagcatattc
     4081 gagaagtgag attgagaggc tcaattaacc gagcggcaag ttttaaccaa gctaattcat
     4141 ttgcaaaaaa tccgacaaaa acgaccgctt cctttttaaa cgatgatact tggaaaatgg
     4201 ggcagaaagt gaaccacgct aaatttggac aaggcacgat tattaatgtg gagggctcag
     4261 gtgaagccac ccgtttgcaa gttgcctttg tcggcaacgg cattaaatgg ctgattgcca
     4321 aaatggcaaa tttagagagg atttaggcga ataaaaagtt ttttgcagga atatagatat
     4381 aaggggcgag atatattgcc ccttaacctt aaactaaggg taaataaaat taaataatgc
     4441 aagctagata taatgtaaag tatcagaaat aagatattaa tttaagataa agaatag
//
LOCUS       DANJWC010000170         4478 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.170, whole genome shotgun sequence.
ACCESSION   DANJWC010000170 DANJWC010000000
VERSION     DANJWC010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.170"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            35..1243
                     /locus_tag="PWX02_002134"
     CDS             35..1243
                     /locus_tag="PWX02_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDL1730104.1"
                     /translation="MSKNLILILNCGSSSLKFAILDPKTGDEKLSGLAEAFHLEDARI
                     KWKLNGEKGNADLGAGATHSEALTFIAEKLLSDELKAGIGAIGHRIVHGGEKFTSSVV
                     ITDEVIKGIEDATQFAPLHNPAHLIGIKEAFRIFPELKDKNVAVFDTAFHQTMPEHAF
                     LYALPYTFYKENGIRRYGFHGTSHFYITSQVAELAGKSVEQTNAIICHLGNGGSVSVV
                     RNGKCIDTSMGLTPLEGLVMGTRSGDLDPAIISFLYNNLGMSMDEIDTTLVKKSGLLG
                     LTGVTSDCRYAEDNYNDVSKPEAKRALDVYSYRLAKYIGSYMAILGDDHLDAIAFTGG
                     IGENSGLVRELTLNHLKLFGIKLDQERNLAARFGKDGVITADESTFKAVVIPTNEELV
                     IAQDTAKLAL"
     gene            1296..3431
                     /gene="pta"
                     /locus_tag="PWX02_002135"
     CDS             1296..3431
                     /gene="pta"
                     /locus_tag="PWX02_002135"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005542370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDL1730105.1"
                     /translation="MSRTIILIPISTGVGLTSVSLGLVHALEQKGSKVGFLKPISQPI
                     SGEDTLDRSTTIIRAEQTTEVGEPFMLSEAETLIGQNQTDVLLEKVVERHQKLSKNNE
                     IVIVEGLIPTRKNSYANSINYEMAQALDAEIVLVAAPASDTPAQLKERVEAAASQFGG
                     RNNPNLLGVIINKFNAPVDDSGRTRPDLTEIFDSFQHSNNNVSEVEHLFAKSPIKLLG
                     CISWKAELIATRAIDVAKHLGASIINEGEIKARRIRGVTFCARNLPNMVDHFKAGSLL
                     VTSADRPEVLVAASLAVMNGIEIGAILLTGGYKIDSSIAKLCQQAFETGVPVFRIEGN
                     TWQTALSLQSFSLEVPVDDKERIAAIKEYMASKFNVGFIDEVSKAATRARRLSPAAFR
                     YQLTEYARQAKKRIVLPEGDEPRTVKAAALCAERGIAECVLLANPTDVQRVAEAQGVV
                     LGKGVTIIDPASIRENYVDRLVELRKAKGMTEVVAREQLLDTVVLGTMMLEANEVDGL
                     VSGAVHTTANTIRPPMQIIKTAPGSSIVSSIFFMLLPDQVLVYGDCAVNPDPTAEQLA
                     EIAIQSAESAKAFGIDPRVAMISYSTGTSGSGADVEKVKEATRIAQEKRPDLIIDGPL
                     QYDAAIMEDVARSKAPNSPVAGKATVFVFPDLNTGNTTYKAVQRSADLVSIGPMLQGM
                     RKPVNDLSRGALVDDIVYTIALTAIQATQ"
     gene            3545..4225
                     /gene="can"
                     /locus_tag="PWX02_002136"
     CDS             3545..4225
                     /gene="can"
                     /locus_tag="PWX02_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HDL1730106.1"
                     /translation="MEKIERLFANNHAWATKMKDEQSDYFKELAEHQKPTYLWIGCSD
                     SRVPAEKLTGLGPGELFVHRNVANMVIHTDLNCLSVVQYAVDVLEIEHIIICGHTNCG
                     GINAAIAQKELGLINNWLLHIRDIWFKHSHLLGKLPYEDRSNVLTRLNVAEQVYNLGQ
                     SSIIKTAWEQGKSLSLHGWVYDVTDGFLIDQGVMATSRETLEITYRNAIAKITTEANE
                     KIVLNDEN"
BASE COUNT         1422 a          818 c          890 g         1348 t
ORIGIN      
        1 tcaaactcat ttcataaaat aaggtaattt gaccatgtct aaaaacttaa tcttaatcct
       61 taactgcggt agttcatctc ttaaatttgc tattttagat cctaaaacag gtgatgaaaa
      121 attatctgga cttgctgaag cttttcattt agaagatgca cgtatcaagt ggaagttaaa
      181 cggggagaaa ggtaatgctg atttaggtgc aggtgccaca catagtgaag cacttacctt
      241 tattgctgaa aaattgttat ctgacgaatt aaaagcaggt atcggcgcga ttggtcatcg
      301 tattgttcat ggcggtgaaa aattcacctc atcagttgta attaccgatg aagtgattaa
      361 aggtattgaa gatgcaacgc aatttgcacc attacacaac cctgcgcatt taattggtat
      421 taaagaagca ttccgcattt tccctgaatt aaaagacaaa aacgtagctg tatttgacac
      481 tgcattccat cagacaatgc cggaacacgc attcttatat gcacttccat atacattcta
      541 taaagaaaat ggtattcgtc gctacggttt ccatggcaca agccacttct atatcacctc
      601 acaagttgca gaattagcag gtaagtctgt tgagcaaacc aatgccatta tttgccactt
      661 aggtaacggt ggttctgttt ctgttgtacg caatggtaaa tgtattgata cctcaatggg
      721 tttaactcca ttagaaggtt tagtaatggg tactcgctct ggtgacttag accctgcgat
      781 tatctctttc ttatacaata acttaggtat gtcaatggac gaaattgata ctactctagt
      841 aaaaaaatct ggtcttttag gtttaactgg cgtaactagt gactgtcgtt atgcggaaga
      901 taattacaat gacgtatcaa aaccggaagc aaaacgtgct ttagatgtat atagctatcg
      961 tttagctaaa tatattggtt catatatggc aattttaggc gatgaccact tagatgctat
     1021 cgcatttaca ggtggtatcg gtgaaaattc aggcttagta cgtgaattaa ccttaaatca
     1081 cttaaaatta tttggcatta agttagatca agagcgtaac cttgctgccc gttttggtaa
     1141 agatggagtc attacagcgg acgagtcaac atttaaagct gttgttatcc caactaatga
     1201 agaactcgtg attgcacaag ataccgctaa attagcatta taatcatagg gggcagatat
     1261 tctgcccttt cactttcttt taatttggaa atattatgtc tagaacgatc attcttatcc
     1321 ctatttcaac tggtgttggt ttaaccagtg ttagcttagg tttagtacat gctttggagc
     1381 aaaaaggttc taaagtagga tttttaaaac cgatttctca acctatcagc ggtgaagata
     1441 cgttagaccg ctcaacaacg attatcagag cagaacaaac aacagaggtt ggtgagccgt
     1501 ttatgttaag cgaagctgaa acattaatcg gtcaaaacca aacggatgta ttgttagaaa
     1561 aagtggtgga acgccaccaa aaattatcaa aaaataatga aattgtcatt gttgaaggtt
     1621 taattccaac gcgtaaaaac tcatacgcta acagcattaa ctatgaaatg gctcaagccc
     1681 ttgatgctga aattgtatta gttgcagcac cggcctcaga tacaccagct caattaaaag
     1741 agcgagttga agctgccgct tcacaatttg gtggacgtaa caacccaaat ttactgggcg
     1801 ttattatcaa taaattcaat gcaccagtag atgattcagg tagaacccgt ccagatttga
     1861 ctgaaatttt tgattcattt cagcactcaa ataataatgt ttcagaagtc gagcatcttt
     1921 ttgctaaaag tccaattaag ctgttaggct gtattagctg gaaagcagaa cttattgcta
     1981 cacgagcaat tgatgttgca aaacatttag gcgcttcaat tattaatgaa ggcgaaataa
     2041 aggctcgtcg gattcgcggt gtgacattct gtgcacgtaa cttaccaaat atggtcgatc
     2101 attttaaagc cggtagttta ttagttacct ctgcggatcg tcctgaagtg ttagttgctg
     2161 catcgttggc ggtaatgaat ggcatcgaaa ttggtgctat tttactaacc ggtggttata
     2221 aaattgatag ctcaattgca aaactttgcc aacaagcatt tgaaaccggt gtgcctgtat
     2281 tccgtattga aggtaacact tggcaaaccg cattaagcct acaaagcttc agcttagaag
     2341 tgcctgtaga tgataaagag cgtattgctg caattaaaga atatatggca agcaaattta
     2401 atgtaggttt cattgatgag gtttctaaag ccgctactcg tgcaagacgt ttatcacctg
     2461 cagcattccg ttaccaatta accgaatatg cccgtcaagc gaaaaaacgc attgttttac
     2521 ctgaaggaga tgagcctcgt actgtgaaag cggctgcatt atgtgctgag cgtggcattg
     2581 cagaatgtgt gttattagct aacccaacag acgtacaacg tgttgctgaa gcacaaggcg
     2641 tggtattagg taagggcgta acgattattg accctgcgag cattcgtgaa aattatgttg
     2701 atcgcttagt tgaattgcgt aaagcaaaag gaatgactga agttgttgct cgtgagcaac
     2761 ttttagatac agttgtattg ggtacaatga tgcttgaagc taatgaagtg gacggtttag
     2821 tctcaggcgc agttcacaca accgctaaca ctattcgccc acccatgcag atcattaaaa
     2881 ctgcaccggg aagctctatt gtatcttcta tcttctttat gctactacct gatcaagtat
     2941 tggtatatgg tgactgtgcg gtaaaccctg acccaactgc agaacagcta gcggaaattg
     3001 cgattcaatc agcagaatct gccaaagcct ttggtattga ccctcgtgtt gcaatgatct
     3061 cctactctac cggtacatca ggttcaggtg cggatgtaga aaaagtgaaa gaagcaactc
     3121 gtattgccca agaaaaacgt ccagatttaa tcattgatgg cccattacaa tatgatgcgg
     3181 cgattatgga agatgttgcc cgctcaaaag caccaaattc acctgtagca ggtaaagcaa
     3241 ctgtatttgt cttcccggat ttaaatactg gtaataccac ctataaagcg gtacaacgtt
     3301 cggcagattt agtctctatc ggcccaatgt tacaaggtat gcgtaaacct gtaaatgact
     3361 tgtcccgtgg ggctttagtt gacgacatcg tttatactat tgcgttaact gcgattcaag
     3421 caacgcaata attccctccc aaatgaaacc aaatgccctt aaaactaagg gcatttttat
     3481 tttttaccta cccgactttt caatttaatc atataatcaa gattagtgtt aaaaaggaga
     3541 atttatggaa aaaattgaac gcttatttgc caataaccac gcctgggcaa ctaagatgaa
     3601 agatgaacaa tcggattatt ttaaagaatt agcggaacat caaaagccaa cctatttatg
     3661 gattggctgt tccgatagtc gtgtgcctgc tgaaaaactc acagggttag gccccggaga
     3721 attatttgtt catcgaaatg tagcaaatat ggtgattcac actgatctta attgtctgtc
     3781 ggttgttcaa tatgcggtag atgtattaga aattgagcat attattatct gtggtcacac
     3841 taattgtggt ggtatcaatg cggcaattgc acaaaaagaa cttggtttaa ttaataactg
     3901 gttgttacat attcgtgata tctggtttaa acacagccat ttactaggca aacttcccta
     3961 tgaagatcga tcaaatgtct tgacccgatt aaatgttgca gaacaagttt ataatttagg
     4021 tcagtcctct attattaaaa ccgcttggga acagggtaaa tccctctcgc tacacggttg
     4081 ggtatacgat gttacagatg gtttcttgat tgaccaaggt gttatggcta ctagccgaga
     4141 aacattagaa attacctatc gtaatgcaat agctaaaata acaaccgaag caaacgaaaa
     4201 aatagtttta aacgatgaaa attagccatt gtaagcggtt aaatttatcg atttttttac
     4261 aatcttttta taaaaaacca ttgtttgatg gttttttatt ttcctgattc ctgttatcgt
     4321 caaaaaagtt ttaaatttca gcaaaagtgt aaaatataga tttaaaaatc aaaaacatac
     4381 aaatatatag atttattttt ataaaatccc aactttttaa gataaaaaag aatataaatc
     4441 tagcaaatct agcatttgat cccataataa gaactaga
//
LOCUS       DANJWC010000171         4422 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.171, whole genome shotgun sequence.
ACCESSION   DANJWC010000171 DANJWC010000000
VERSION     DANJWC010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.171"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            217..1506
                     /locus_tag="PWX02_002137"
     CDS             217..1506
                     /locus_tag="PWX02_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730107.1"
                     /translation="MHPKFKLTLAALAVSVVLTGCKSSQGPSVVAKNSDDRVIKLNAE
                     TEDKKEETPAEQPRPAPQKPQVTPPAPLLPTTPKLEEKPNKEMVETTPKPEEKPNEKM
                     VEEGNNGEDEIKVITSITPVNTDSKGSTWRVVPLSQTGGVVNLSTARGDHTDYRLTDE
                     NSPLGSSQEFGKTEYNYPIGFELGKNEQNSPFIKTLNKRGDKYLGQHSGTYKDDKLVG
                     NSQKINYLYVNQPYSSYGALFTDANNSNLFHVQLKTGRDSNGISAESEGSNANSAEYS
                     VYTLQGKTGKWNNEVLGDAEYKGNLIARIEKQMDGKTVIEAPKVDGEVTLSLHLDNNW
                     ANNTLEGKVTSKTLGEIGLDKTKLAPAVRLPTNEISFYGTASATDHEGLKGEYSATFA
                     GKTLNDAVGSIELENEDANNGDVSKYNAVFGVMKVEK"
     gene            1566..2978
                     /locus_tag="PWX02_002138"
     CDS             1566..2978
                     /locus_tag="PWX02_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL1730108.1"
                     /translation="MKKIALLAFFPTLLFARDPNTTPAHLEHTAQPTTAPQAVAIEQS
                     PPIFNEAELRANQALTEELLTQAIYSRQLANMEKLTAIYQDFTQIDPILLRYAQGKIS
                     LLKGDYAQAIQHFQTILAKQPKLNTVRIELAIALLYDQQLNQAQAQFEKAKSAEHLPP
                     KVHALIEQYQQHLAKKQEWKFSFSANYVRDGNINRTSKQPNIEQTGYVKQPNLLPQTA
                     HGVHYAFGLQRDFNLFDSHYVSFDNQTQGELYWDNHDYDEITNRTLFGYAYKKSDQTT
                     RLLPFYEKRWKHHRSERWTNGIRLEHAQWFSPHWQLATALEYGKKRYFDLPIYNGSYK
                     LASATLLWLRNPQQFFFVGTDFNQDKTQLKQYSSDTKGIRFGWQREWQALELSSRLTL
                     GYTDRRYKDTAILGGKLNLGRIRHDRVQSANLTLWKRDWHWLGLMPKLQFSYAKHRSN
                     LPSLYAYTDKNINLIIESRF"
     gene            complement(3228..3506)
                     /locus_tag="PWX02_002139"
     CDS             complement(3228..3506)
                     /locus_tag="PWX02_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin"
                     /protein_id="HDL1730109.1"
                     /translation="MTEAKLELGKVETRLRNEGKTKEEIAQHPEYLQLKRDLNDKQQT
                     FDNAYGTGSKTKRAVDAVTAALQGLAAKDSGQAAVGLASPYLNEQIKK"
     gene            complement(3666..4262)
                     /locus_tag="PWX02_002140"
     CDS             complement(3666..4262)
                     /locus_tag="PWX02_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HDL1730110.1"
                     /translation="MADSYRNQTKEKNHSASVGVFVSFNGDSYGIGLEASGSVGKGKE
                     NSESETWQNNQLQAGKLVTNSANGKLLLDATTVKANRWEGEVQDFEAKSRQDIIKYSS
                     EQVQAGGSASLALGTSSANVNAAYNSAKLNTAQVEKQTSIDIGKGGMDVKVKKNAHFD
                     GAVMTSQAEKENNRFQAGTLTTGDWRKYRYRPNPRPKC"
     gene            complement(4196..>4422)
                     /locus_tag="PWX02_002141"
     CDS             complement(4196..>4422)
                     /locus_tag="PWX02_002141"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730111.1"
                     /translation="IFETNPPVLYTSIIGHSADFFGGALYNGEIAKAQWVDAQHLQKG
                     YRLLAESGEWQIVTATKPKRKTTVRAWVCL"
BASE COUNT         1315 a         1046 c          878 g         1183 t
ORIGIN      
        1 aagcgggcta aattgggcag aaattagcaa agccagtaat tttttattaa acacaatcat
       61 ctccttaaaa attttaaggt taataaatta ctgtaaaaaa gagtttttta caatagacca
      121 taaaaaattt atgtaatttt taacttacct acttgtcaaa aggtaaattt atccattaaa
      181 attttatggt ttttataagg aaaggagaaa atatctatgc atcctaaatt caaactaacc
      241 cttgccgcat tagcggtttc tgttgtttta accggctgca aatccagcca aggtccttct
      301 gtggtagcga aaaattcaga tgaccgagta attaaactca acgccgaaac ggaagataaa
      361 aaagaagaaa ccccagcaga gcaacctcgc cctgcccccc aaaaaccaca agtcacacca
      421 ccagctccgt tattgcctac aacacctaag ctagaggaaa aaccaaacaa agaaatggta
      481 gaaacaacac ctaagccaga agaaaaacca aacgaaaaaa tggtagaaga agggaataac
      541 ggtgaagatg aaattaaggt cattacttct atcacgccgg tgaataccga cagcaaaggc
      601 tctacgtggc gtgttgtacc gctcagccaa acaggtggcg tggtgaattt aagcaccgca
      661 agaggcgatc acaccgatta tcgcttaacg gacgaaaact cccctctcgg ctcaagccaa
      721 gagtttggga agacagaata taactaccca attggttttg agctgggaaa aaatgagcaa
      781 aacagcccat tcattaaaac actcaacaaa cggggtgata agtatctcgg tcagcattcc
      841 ggcacttata aagacgataa attggtcggc aacagccaaa aaatcaatta tctctacgtc
      901 aatcagccat attccagtta tggtgcactc tttaccgatg caaacaacag caatctgttc
      961 cacgttcagc taaaaaccgg gcgtgacagc aatggtattt cagcggagag tgaaggcagt
     1021 aatgcgaata gtgccgaata tagcgtctat actctgcaag gcaaaaccgg taaatggaat
     1081 aatgaagtgc tgggcgatgc agagtataaa ggtaacctaa ttgctcgtat tgaaaaacag
     1141 atggacggaa aaacggtgat cgaagcacca aaagtggatg gtgaggttac actcagccta
     1201 cacttagata acaattgggc aaataatacg ttagaaggta aggtaaccag taaaacactg
     1261 ggtgaaattg ggctggataa aaccaaactc gcacctgccg taagattacc gactaatgaa
     1321 atcagcttct acggcaccgc aagtgctact gaccacgagg gtttaaaagg cgaatattca
     1381 gcaacctttg ccggtaaaac actcaatgat gccgtaggtt caattgaatt ggaaaatgaa
     1441 gatgccaata acggtgacgt cagcaaatat aacgccgtct tcggtgtaat gaaagttgaa
     1501 aaataacctc atattacgat aatcatagag ggctggcaac agccctttgt ttttaaggac
     1561 gaacaatgaa aaaaattgct ttgctggctt ttttcccgac actactcttt gcccgagatc
     1621 cgaatacgac accagctcat cttgagcata cagcacagcc aacaaccgca ccacaagctg
     1681 ttgcgattga acaatccccc cccattttta atgaagcgga attacgggca aatcaggcat
     1741 taaccgagga gctgctgact caagccattt atagccgtca attagccaat atggaaaaat
     1801 tgaccgctat ttatcaagat tttacgcaga ttgaccctat tttactgcgt tatgcacaag
     1861 gtaaaatttc cctactgaaa ggcgattacg cacaggcaat tcagcacttc caaaccattc
     1921 ttgccaaaca acccaaacta aacactgtaa gaattgagct ggcgattgct ctattgtacg
     1981 atcagcaact taatcaagcc caagcacaat ttgaaaaagc caaaagtgcc gaacatctac
     2041 cgcctaaagt tcacgcatta attgagcaat atcagcaaca tctggcaaaa aaacaggaat
     2101 ggaaatttag tttttcagcc aattatgtgc gagacggcaa cattaaccga acctcgaaac
     2161 agccgaacat tgagcaaacc ggctatgtaa aacaacctaa tttgttgccc caaaccgcac
     2221 atggtgttca ctatgcgttt ggattacaga gagatttcaa cctgttcgac tcacactatg
     2281 tgagttttga caaccaaacc caaggtgaac tttattggga taaccacgat tatgatgaaa
     2341 ttaccaatcg caccttgttt ggatacgctt acaaaaaatc cgaccaaacc acccgcttgt
     2401 taccgtttta tgaaaaacgc tggaaacatc accgttcgga acgctggaca aacggaattc
     2461 gacttgaaca tgcacaatgg ttttcaccgc attggcaact cgccaccgca ttggaatacg
     2521 gcaaaaaacg ttattttgat ttgccgattt ataacggcag ctacaaactt gcctctgcca
     2581 cattgctttg gttacgcaat ccacaacaat ttttctttgt cggcactgat ttcaatcaag
     2641 acaaaactca gttaaaacaa tatagctctg atactaaagg catacgcttt ggctggcaac
     2701 gagaatggca agccctcgag ctttccagtc gcctcacgct tggctatact gacagacgtt
     2761 acaaagacac cgctatattg ggaggaaaac ttaatcttgg cagaatccgc catgaccgtg
     2821 tgcaaagtgc taacctcacg ctttggaaac gagattggca ctggctcggc ctgatgccga
     2881 agctgcaatt tagttacgcc aaacaccgta gcaacctgcc gagcctctat gcctacaccg
     2941 ataaaaacat caatttaatt attgaaagtc ggttctagct ccataaaaac aacaagcggt
     3001 taaaattcct agaaattttg caaattgcaa aaaatgtaga aaatttaacc gcttgtatct
     3061 atactgcagc ttcttttcct tcagtataaa ttaatataca ccgttgcaac aataatggct
     3121 gctccctgtc atcaccttga actgaaaatt gctcaaggtg gatatgacta tcgcagccgc
     3181 ttccaccgca cccagtaatg tatgggcaag caggttggct tgggtatcta ctttttaatt
     3241 tgttcgttga gatatggcga agctaagcct actgctgctt gtcctgaatc tttcgccgct
     3301 aaaccttgta aggctgccgt caccgcatcc accgcacgct tggttttgct gcccgtgccg
     3361 taggcgttat caaaagtctg ctgtttatcg tttaaatccc gttttaattg gagatattca
     3421 ggatgttgag cgatttcttc ttttgttttt ccctcatttc tcaaacgggt ttccactttg
     3481 cccaattcca gcttcgcttc cgtcactttt tcacgctcac tatgcgtgta aatctgataa
     3541 gccttctgca aatctcgcct atgcccttca ccagctcctg tgtttctttc acctcactaa
     3601 tatcgtgttt cgtcactttt tgattggcgt tttgcgtatc tcggttaagc tgattgagcg
     3661 tgctttcagc atttgggtct tggattaggg cgatagcgat attttctcca atcgccggtg
     3721 gtcagcgtgc cggcttggaa gcggttgttt tctttctccg cttggctggt cattaccgca
     3781 ccgtcaaaat gggcattctt tttcacttta acgtccattc ctcccttgcc aatgtcgatt
     3841 gaggtttgtt tttctacctg tgccgtgttc aacttggctg agttgtaagc cgcatttaca
     3901 ttggcactgc ttgtacccaa ggcaagtgaa gcactgccac ccgcttgcac ctgttcgctg
     3961 ctgtatttga tgatgtcttg tcggcttttc gcctcaaaat cttgtacctc gccttcccag
     4021 cgattggctt tgacagtcgt ggcatccagc aagagtttac cgtttgcaga attggtaact
     4081 aatttacccg cttgtaattg gttgttctgc cacgtctcgc tttcgctgtt ctccttacct
     4141 ttgccaacac tgcccgacgc ttccagccct atgccgtagc tgtcgccatt gaagctcaca
     4201 aacacaccca cgctcgcact gtggtttttc tctttggttt ggttgcggta actatctgcc
     4261 actcaccact ttcagcaagt aagcgatagc ctttttgcaa atgttgagca tcaacccact
     4321 gagcttttgc tatctctcca ttgtataatg cccctccgaa gaaatcggca ctatgcccga
     4381 taatacttgt gtaaagaacg gggggatttg tttcaaatat ta
//
LOCUS       DANJWC010000172         4292 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.172, whole genome shotgun sequence.
ACCESSION   DANJWC010000172 DANJWC010000000
VERSION     DANJWC010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.172"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..238
                     /locus_tag="PWX02_002142"
     CDS             <1..238
                     /locus_tag="PWX02_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDL1730112.1"
                     /translation="ILALGLFQIANFGQNPPVCRAKAFNIIGSNWALGILLFSGNLYL
                     LALNVPTIHWLTPIGGLCFMLGWIALIYISVRKA"
     gene            238..1326
                     /gene="rlmM"
                     /locus_tag="PWX02_002143"
     CDS             238..1326
                     /gene="rlmM"
                     /locus_tag="PWX02_002143"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HDL1730113.1"
                     /translation="MFNKLALYCRAGFEKEVAGEITDKAAQQGIFGFANLKENSGYVI
                     FECYQAGEADRLAQALKFEQLIFVRQMLVVGDLLQNLSENDRISPILAQYAELNPRQS
                     SDVFVETPDTNEAKELLTFCRKFTVPLRSALKKQGWLGKSENMQGSVSLHILFTRPGA
                     CYVGYAYNHNKSPFFMGIPRLKFPAEAPSRSTLKLEEAILTFIPQAEEKKRFNDTMVG
                     VDLGACPGGWTYQLVKRGVFVYAVDHGKMAASLHETGRIEHCPEDGFKFQPPKRKQID
                     WLVCDMVEQPIRISKLIAKWLTNGWCRETIFNLKLPMKKRYQEVQLCLNFLEEELTKH
                     GFWFKMQAKHLYHDREEITVHIAVMGQK"
     gene            1394..1867
                     /locus_tag="PWX02_002144"
     CDS             1394..1867
                     /locus_tag="PWX02_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730114.1"
                     /translation="MMKNLPLSMADTLAVIYPAFDFAADDKPATEQQISLLNFRITMA
                     NQLLIACIETIEKNPNFWAEIDLNDFKKYLNQNKVVIFDTQQYFTDGSRTMAILDNMK
                     SNIRKIEQQLSQATAFNFDLERMKERLNDTFHEVPKDITNVNEFRNWLKGVANGH"
     gene            1857..2234
                     /locus_tag="PWX02_002145"
     CDS             1857..2234
                     /locus_tag="PWX02_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730115.1"
                     /translation="MGIKVLFGTKISAELQYLPEKDLVKILQFKQHVEIYGLENLAGR
                     NKSSDDVPTNDPYWAEKVAKAQKYSLWHYHIGIPEYDTSQGFGNYTSEYILHYVKGEN
                     FIQIVDFTSHPPFKLPSESYLQN"
     gene            2285..3097
                     /gene="dapB"
                     /locus_tag="PWX02_002146"
     CDS             2285..3097
                     /gene="dapB"
                     /locus_tag="PWX02_002146"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDL1730116.1"
                     /translation="MTLKIGIVGAGGRMGRQLIQAVHNAQGVELGSAFERKGSSLVGS
                     DTGEVAGVGTLGVVISDDVAQNVAGFDLLIDFTRPEGTLEHLDICVAHHKQMVIGTTG
                     FDDAGKAAIKAASEKIGIVFASNYSVGVNLVFKLLEKAAKVMGDYCDIEVIEAHHRHK
                     VDAPSGTALSMGEHIAKTLGRDLKTHGVFERNGITGERKHDEIGFATIRAGDVVGEHS
                     VWFADEGERVEIAHKASSRMTFANGAVRAAKWLNGKQNGLFDMTDVLDLNNL"
     gene            3114..4247
                     /locus_tag="PWX02_002147"
     CDS             3114..4247
                     /locus_tag="PWX02_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis PLP-dependent protein"
                     /protein_id="HDL1730117.1"
                     /translation="MTKFNNIETTLVQIGNRTDPRTGAVAMPIILSTAYGRDGLGEST
                     GWDYTRTKNPTRAVLEQGIADLEGGDAGFAMASGMAAIQLVMSLFKAPDEWIISSDVY
                     GGSYRLFDFSHKNHSTLKPVYVNTADLAAIEAAITPNTKAIFVETPSNPLMEECDVAV
                     IAKIAKKHNLMLIVDNTFLTPALFRPIEHGADIVIHSATKYLSGHNDVLAGLIVAKDS
                     DATKVNGQNLTERLFYFQNCAGAVLSPFDSYLAVRGMKTLALRMERHQSNATELAKFL
                     AEQPEIDSVLYSGKSGMLSFRLQKEEWVAKFLKAIKLITFAESLGGAESFITYPATQT
                     HMDIPEAERITRGITNNLLRFSVGLEHVEDIKADLLQAFKQLK"
BASE COUNT         1317 a          750 c          971 g         1254 t
ORIGIN      
        1 cattttagct ttaggtttat tccaaattgc aaattttggg caaaatccac ccgtttgtag
       61 agccaaagcc tttaacatca tcggaagcaa ttgggcgtta ggcattctgc tttttagtgg
      121 caacctgtac ttactggcgt taaatgtgcc gactattcat tggcttacgc ctattggtgg
      181 tctctgcttt atgcttggtt ggattgcgtt aatttatatc agtgtgagaa aagcctaatg
      241 ttcaataaat tagccttata ttgccgagca gggtttgaaa aagaggtagc aggcgaaatc
      301 acggataaag ctgcacaaca gggcattttt ggctttgcca accttaaaga aaatagtggc
      361 tatgtgattt ttgagtgcta tcaagcaggg gaagcagatc gattagccca agcgttaaag
      421 tttgaacagc ttatttttgt tcgccaaatg ttggttgttg gggatttgtt gcaaaatctg
      481 tctgaaaatg atcgaatttc gccgatttta gcccaatatg ccgaacttaa tcctcgccaa
      541 agttctgatg tatttgtcga aacaccggat accaacgaag cgaaagaatt attaactttc
      601 tgtcgtaaat ttactgtgcc attacggtcg gcattgaaaa aacaaggttg gttggggaaa
      661 tcggaaaata tgcagggtag tgtgagcttg catattttat ttactcgtcc gggagcttgc
      721 tatgtgggct atgcgtacaa ccataataaa tcgccgtttt ttatggggat tccccgttta
      781 aaattcccgg ctgaggcacc tagtcgctca acgcttaaat tagaagaggc gattttaact
      841 tttattccgc aagcagagga gaaaaaacgc tttaatgata cgatggtagg ggtcgatctt
      901 ggggcttgtc cgggtggttg gacttatcaa ttagtcaaac gtggtgtttt tgtctatgca
      961 gtcgatcacg gcaaaatggc agcaagttta cacgaaaccg gaagaattga gcattgccct
     1021 gaagatgggt ttaaattcca accgcctaag cgtaaacaaa tcgattggct ggtttgtgat
     1081 atggtagagc agccgattcg tatctcgaaa ctcattgcaa aatggctgac taacggttgg
     1141 tgtcgagaaa cgattttcaa cctaaaactg ccgatgaaaa aacgttatca agaagtgcaa
     1201 ctatgcctca atttcttgga agaagagctg acgaaacacg ggttttggtt caaaatgcaa
     1261 gctaaacacc tctaccacga ccgtgaggaa attacggtac atattgcggt gatggggcaa
     1321 aaatagcata aaatgaataa gttgtttttc taagtaatcc agtagataat gacaacttat
     1381 tccgtaagga gagatgatga aaaatttacc cttatctatg gctgacacgc ttgcagtgat
     1441 ttatccggca tttgatttcg cagctgatga taaacctgca acagagcagc aaatctcatt
     1501 gttaaatttt cgtattacaa tggcaaatca attattaatt gcctgtattg aaactatcga
     1561 aaaaaatcca aacttttggg ctgaaattga tttaaacgat ttcaaaaaat acctaaatca
     1621 aaataaggtg gtgattttcg atacccaaca gtattttact gatggctcaa ggacaatggc
     1681 tatattggat aatatgaagt ccaatattcg caaaattgaa cagcaactct ctcaagcaac
     1741 cgcatttaat tttgacttag agcgaatgaa agagcggtta aatgatactt ttcacgaagt
     1801 gcctaaagat ataacgaatg ttaatgaatt tagaaactgg ttgaaaggtg ttgcaaatgg
     1861 gcattaaagt tttatttggt acaaaaattt cagccgaatt gcaatatttg cctgaaaaag
     1921 atctagtcaa aatcctgcag tttaaacaac acgttgaaat ttatggattg gaaaatctag
     1981 ctggtcgaaa taaaagttct gatgatgtgc caacgaatga tccttattgg gcagagaagg
     2041 tggctaaagc acaaaaatat tctttatggc attatcatat cggcattcca gaatatgata
     2101 ccagtcaagg atttggtaat tatacttctg agtatatttt acattatgtg aaaggtgaaa
     2161 actttattca aattgttgat tttacttctc acccaccttt taaattgcca tcagaaagct
     2221 atttgcaaaa ttaattaaaa atgtaaccgc ttgtaaaaaa caaatataaa aaggaagaac
     2281 aaatatgaca ttaaaaattg gaatcgtcgg tgccggtggg cgaatggggc gtcaattaat
     2341 tcaagccgtc cataatgcac aaggcgtaga attagggtct gcttttgagc gtaaaggttc
     2401 atcattagta ggtagtgata ctggtgaagt agccggggtt ggtacattag gtgtggtgat
     2461 ttctgatgat gtcgcccaaa atgtagcagg ttttgattta ctaattgatt ttactcgccc
     2521 tgaaggcacg ctagaacatt tggatatttg tgtggctcat cataaacaaa tggtgattgg
     2581 cacaaccggt tttgatgatg caggtaaagc tgcaatcaaa gcagcgagtg aaaaaatcgg
     2641 tatcgttttt gcctcaaact acagcgttgg ggtgaatctg gtgtttaaac tgctcgaaaa
     2701 agcagcaaaa gtaatgggcg attattgtga tattgaagtg attgaggcac accatcgcca
     2761 taaagttgat gcaccatcag gtacggcttt atcaatggga gaacatattg ccaaaactct
     2821 tgggcgtgac ttgaaaactc atggcgtatt tgaacgtaac ggtattacag gcgaacgcaa
     2881 acatgatgaa atcggctttg ctactattcg tgccggtgat gttgtagggg agcatagtgt
     2941 atggttcgct gatgaaggcg aacgtgtcga aatcgcacat aaagcctcta gccgaatgac
     3001 gtttgccaat ggcgcagtgc gtgctgcaaa atggttaaat ggcaaacaaa atggcttgtt
     3061 tgatatgaca gacgtattgg atctaaataa tctgtaattg aaggataata acaatgacaa
     3121 aattcaataa tattgaaacc acattagtgc aaatcggtaa cagaactgat cctcgtacag
     3181 gggcggtggc aatgccgatt atcttatcaa ctgcttacgg gcgtgatggc ttaggcgaat
     3241 ctaccggctg ggattacacc cgtaccaaaa acccaactcg agctgtatta gagcagggga
     3301 ttgcggattt agaaggcggt gatgccggtt ttgcgatggc atctggaatg gcagcgattc
     3361 agctagtaat gtcattattt aaagcacctg atgaatggat tatttccagc gatgtttatg
     3421 gtggttctta ccgcttgttt gattttagcc acaaaaatca cagcacatta aagccagttt
     3481 atgttaatac agcggattta gcagcgattg aagcagctat tacaccgaat actaaggcga
     3541 ttttcgtgga aacgccgtca aatccattga tggaggagtg cgacgttgct gtcattgcta
     3601 aaattgcgaa aaaacacaat ttaatgctga tcgttgataa tacgttctta accccagcgt
     3661 tattccgtcc gattgagcac ggagctgata ttgtgattca cagtgcgacc aagtatcttt
     3721 cggggcataa tgatgtgtta gccggcttaa ttgttgcgaa agattctgat gctaccaaag
     3781 taaacgggca gaatttgacc gagcgtttat tctatttcca aaactgtgcc ggtgcagtgc
     3841 tgtcgccatt tgattcttac cttgccgtgc gtggtatgaa aaccttggca ttacgaatgg
     3901 aacgccatca gtctaatgcc accgaactgg ctaaattctt agcagagcaa cctgaaattg
     3961 acagcgtgtt atattcaggc aaaagtggta tgttatcttt ccgtttacaa aaagaagagt
     4021 gggtagcgaa atttctcaag gcaattaaat tgattacctt tgcggaaagt ttaggtggtg
     4081 cagaaagttt tattacttat cctgcaacac aaacccatat ggatattccg gaagcagaac
     4141 gtattacacg aggcattacg aataatttac tgcgtttttc cgttggttta gaacacgttg
     4201 aagatattaa agctgattta ctgcaagcct ttaaacagtt gaagtaattt ttttgtattc
     4261 acaagcggtc atattaggta tagattttac ca
//
LOCUS       DANJWC010000173         4159 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.173, whole genome shotgun sequence.
ACCESSION   DANJWC010000173 DANJWC010000000
VERSION     DANJWC010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.173"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..459)
                     /locus_tag="PWX02_002148"
     CDS             complement(<1..459)
                     /locus_tag="PWX02_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDL1730118.1"
                     /translation="MILADLSRDDYLKSLPPVFARLCKKLKSLDLVNLELGWQELENG
                     IRMNVMSFETTPAEGKKAELHRKFIDIQLLIDGEEMIEYGLSQPDLSKYDEYRDEDDY
                     QLTAEIDDKNNLILKPNMFAIFLPYEPHKPGNSVNGNKTLKKLVVKVPVEL"
     gene            complement(706..2208)
                     /gene="ppx"
                     /locus_tag="PWX02_002149"
     CDS             complement(706..2208)
                     /gene="ppx"
                     /locus_tag="PWX02_002149"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HDL1730119.1"
                     /translation="MSSNIAPREFAAIDLGSNSFHMIVARIVNGSIQVLSRLKRRVRL
                     ADGLDENLVLSQDAIDRGIACLALFAKRLEGFSAENVKVVGTYTLRRAINNQAFLEQA
                     KAVFPYPISIISGQQEARLIYSGVSHTQPETGRKLVVDIGGGSTEMTIGDDFTPIRAE
                     SRHMGCVSFAKRFFPQGELTIERFDSAYQLAMEKIEDLMWEYKELGWDYALGSSGTIK
                     TVHQVLIANGYRDGLITESRLQKIIDICLEFKSLNEIKLRGLLDERADVLVPGLAILL
                     ALFHTFRIDVLRYSNGALREGVMYGLEKSFQVGDIRQRTAEALAEQFALDKQQAKRVE
                     HTALILFDQIKTWKNRRQVQDLRSMLKWASLLHEIGIVVNHNNVHRHSAYLIANRDLP
                     GFDFEQQTLLSVLMRYHLKGFKRSDIRSINRYQCRDVLTLVRVFRLAVLLNRSRQATV
                     VPKQLNLVIENNAWQLTFEQGFLANNPLISIDLEEEQKILASTNLQFAVS"
     gene            2404..2733
                     /locus_tag="PWX02_002150"
     CDS             2404..2733
                     /locus_tag="PWX02_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HDL1730120.1"
                     /translation="MSEVNKSFHDMLEYVHLYRLKNKLHRETADNDRKIRDNQKRVLL
                     LDNLTQYITDEMSIEEVRAIISNMRDDYENRVDDYMIRNAELSKQRREIRQKMASHKQ
                     APTNKKA"
     gene            complement(2797..>4159)
                     /locus_tag="PWX02_002151"
     CDS             complement(2797..>4159)
                     /locus_tag="PWX02_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL1730121.1"
                     /translation="AALLMGIGYWIDPDGWGTNSQLAALLIKSGAAIIDNMSILFAVG
                     VAFGMSKEKDGAAALSGLVGYLVITTLLSPGTVAQLKGIAVDQVPAAFGKINNQFIGI
                     LVGVISAEIYNRFYQVELPKALSFFSGKRLVPIVVSFILLYVWPVIFGALVSFGESIK
                     DMGALGAGIYAFFNRLLIPVGLHHALNSVFWFDVAGINDIPNFLGGAKSLAEGKAVIG
                     VTGMYQAGFFPVMMFGLPAAALAIYHSAKPAKKMQVASIMFAAALASFFTGITEPLEF
                     AFMFVAPVLYVVHALLTGLSVYIAASMEWIAGFGFSAGLVDLVLSTRNPLAVQWYMLL
                     AQGIVFAVIYYVLFRVVIQAFNLKTPGREEDSEVQTVNTVSRSEQAAKFVEALGGKEN
                     ITSIDACITRLRLTLNDTAKADETKLKALGSKGNIKLGENGYQVILGPEAEFVADAMK
                     KLN"
BASE COUNT         1340 a          932 c          705 g         1182 t
ORIGIN      
        1 caattcaaca ggtactttca ccaccagttt tttcaatgtt ttgttgccat tcaccgaatt
       61 tccaggctta tgtggttcat aagggaggaa aatagcgaac atatttggct tgaggatcaa
      121 gttattttta tcatcaatct cagctgttaa ttggtagtca tcttcatctc gatattcatc
      181 atacttagat aaatctggct ggcttaaccc gtattcaatc atttcttcgc cgtctatcaa
      241 taattgaata tcgataaatt ttcggtgtaa ctctgctttt ttcccctcag ctggcgtagt
      301 ttcaaatgac attacattca tgcgaatacc attttctaat tcttgccagc ctaattccaa
      361 attaactaaa tctaaacttt ttaatttttt acaaaggcga gcgaatactg gtggaagtga
      421 ttttaaataa tcatctcttg ataaatcggc taatatcata taattctcct aaaaataact
      481 aattctaata atctgctcca tactactaaa atttattaag atacactcca atagaagtag
      541 gataattcgt aaaatatata atctcaataa gcaaacatca aaaatgagaa ggtgatcaaa
      601 ttttaaaaaa tactttttgc aaaaaattag ctaaatttga ccgcttgttc gcaaaaggga
      661 atgcaataaa cgaaaaagga agccttcgct tccttcattt aaatgttaag agaccgcaaa
      721 ctgaagattt gtactcgcca atattttttg ctcttcttct aaatctattg aaatcagtgg
      781 attattcgct aagaagccct gttcaaaggt aagttgccaa gcattatttt caatcaccaa
      841 attcaactgt ttcggcacta cagtcgcttg gcgagaacgg tttaatagca ccgctagccg
      901 gaaaacccgt accaacgtta atacatctct acattggtaa cggttgatac tacgaatatc
      961 cgagcgtttg aatcctttaa ggtggtatcg cattaatacc gatagaaggg tttgctgttc
     1021 aaaatcaaat cccggtaaat ctcggtttgc aatcaaataa gcagaatgac gatgcacatt
     1081 attgtggttt acaacaatac caatctcgtg taataaactt gcccatttca gcattgaacg
     1141 cagatcttgc acttggcggc ggttcttcca agttttgatt tggtcaaata aaatcaacgc
     1201 cgtatgctcc accctttttg cttgctgttt atctaaagca aattgctcag ctaacgcttc
     1261 tgcagtccgt tggcgaatat ccccaacttg gaaacttttc tccaaaccat acattacccc
     1321 ttctcttaaa gccccgttgg aataacgtaa tacatcaatt ctaaaagtgt gaaaaagtgc
     1381 taaaagaata gctaagcccg gaactagcac atctgctcgt tcatcaagta agcctcgtag
     1441 cttaatctca tttaacgatt taaactctag gcaaatatca ataattttct gtaggcggct
     1501 ttctgtaatt aagccatcac gatagccatt ggcaatcagc acttgatgga cggttttaat
     1561 cgtaccggaa gaacctaaag catagtccca acctaactct ttgtactccc acattaaatc
     1621 ttcgattttt tccattgcaa gctgataggc actatcaaaa cgctcaatcg ttaattcccc
     1681 ctgcgggaaa aagcgttttg caaaactcac acaacccata tgacgacttt ccgctcgaat
     1741 cggggtaaag tcatcaccaa tagtcatttc cgttgaacca ccgccaatat ctaccactaa
     1801 ttttctgcca gtttcgggtt gggtatggct aacgcccgaa taaatcagcc tcgcctcttg
     1861 ttgtccggaa ataatggaaa tcgggtaagg aaatactgct ttcgcttgct ccagaaaagc
     1921 ttggttattg attgctctac gtaaggtata agtgccaacc accttcacat tctctgcaga
     1981 gaacccttct aaacgttttg caaataaagc taaacaagca atgcctctat caatcgcatc
     2041 ttggcttaat actaagtttt catcaagccc atctgccagc cgtacacgcc gtttcaaacg
     2101 ggaaagcact tgaatggaac catttacgat acgggctaca atcatatgaa agctgttcga
     2161 gcctaaatca atagctgcaa actctctagg agcaatattt gatgacataa tgaaaccctg
     2221 taccaataac tgaaataagt aagatattac catttttgag ccctagatat cactacaaat
     2281 ttagcgttat tcgcgagaat taatttaaat cataaaatgt gataaatttt tttgacgaat
     2341 tgacggaaaa agaataggat ttacgagctt acttaattca acactacatc aagggagatt
     2401 gatatgtctg aagtgaataa atcattccac gatatgctgg aatatgtgca tctttatcgt
     2461 ctaaaaaaca aactgcaccg tgaaacggcg gataatgatc gcaaaattcg agataatcag
     2521 aagcgtgtac ttttattaga taacctaacg caatatatta ccgacgaaat gtctatcgag
     2581 gaagttcgag cgattatttc caatatgcgt gatgactatg aaaatcgagt agatgattat
     2641 atgatccgta atgcggaact ttccaaacaa cgccgtgaaa tccgccaaaa aatggcctct
     2701 cacaaacaag caccaaccaa taaaaaagca taaaaagcca agcggtctga tttgcaaaaa
     2761 aatatgcaaa tcagaccgct tgtgattaag cagagattaa ttcagctttt tcattgcatc
     2821 tgccacaaat tctgcttccg gtcccaaaat cacttggtag ccattctcgc ctaatttgat
     2881 attacctttt gaccctaacg ctttcaattt ggtttcgtct gctttcgccg tatcatttaa
     2941 ggttaggcgt aagcgggtaa tacaagcatc aatgctggta atattctctt taccacctaa
     3001 tgcttcaaca aatttggcag cttgttcgct acgagaaaca gtattcactg tttgtacttc
     3061 gctatcttcc tcacgccccg gcgtttttaa attaaaggct tggatcacta ctcggaataa
     3121 tacatagtaa attactgcga atacgatacc ttgggccaat aacatatacc actgcactgc
     3181 cagcggattt cgtgtcgata acactaaatc gaccaaccct gcactaaaac cgaaaccggc
     3241 aatccattcc attgaggctg caatataaac agataatccg gttaataatg cgtgaaccac
     3301 ataaagcaca ggtgcaacaa acataaaggc aaattcaagc ggttccgtaa tgccggtgaa
     3361 gaacgaagct aaagcagcag caaacataat agaggctact tgcattttct tagccggttt
     3421 cgcactgtgg taaatagcta atgctgccgc aggtaagcca aacatcatca ccgggaagaa
     3481 accagcttga tacatacctg ttactcctat cactgctttg ccctcagcca atgattttgc
     3541 cccacctaag aagtttggaa tatcattaat gcctgcaaca tcaaaccaga aaaccgagtt
     3601 taatgcgtgg tgtaatccaa ccggaattaa taaacgatta aagaaagcat aaatacctgc
     3661 ccctaacgcc cccatatctt tgatagattc cccaaaagag actaaagcac caaaaataac
     3721 cggccagaca taaagcaaga taaatgacac cacaatcggc accaatcgtt taccgctaaa
     3781 gaaagacaat gctttcggta actctacttg gtagaaacgg ttataaattt cggctgaaat
     3841 aacccctact aagatcccga taaattggtt gttgatttta ccgaatgctg caggcacttg
     3901 atcaactgcg atccctttta gctgagcaac ggtccccgga gacaacaaag tggtaattac
     3961 taaatacccg actaaaccgg ataatgctgc tgccccatct ttttcttttg acataccaaa
     4021 tgccacaccc acggcaaata gaatagacat attatcaata attgccgccc cagatttaat
     4081 cagcaatgcc gctaattggc tatttgttcc ccagccgtcc ggatcaatcc aatagcctat
     4141 acccatcaat aatgctgct
//
LOCUS       DANJWC010000174         4102 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.174, whole genome shotgun sequence.
ACCESSION   DANJWC010000174 DANJWC010000000
VERSION     DANJWC010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.174"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..1141
                     /locus_tag="PWX02_002152"
     CDS             <1..1141
                     /locus_tag="PWX02_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDL1730122.1"
                     /translation="SGIVTIIQCRGIGPIGIRLPSVMGTSFTFVAAALAIGFSESGIA
                     GIMGASLVGSLVMIIGSFFMPYIRKLFPPVVTGTVIMMIGLSLIPVAVDWFAGGQRTD
                     PHYATPENLIMASFVLIIVVALVQWGKGIFSAAAIVIGMMIGYIVSLALGWVSFNGVK
                     EAQLFALPQPLHFGLEFPISGIIGMSIAYLVTIVESSGNFLALGNATKTEITGKHLRG
                     GVLCDGLGSAFAAIMSTTPFSSFSQNIGVISLTGVASRYVVAFTGALLVLAGLFPVFG
                     ALIVSIPLPVLGGAGLMMFAMIIAAGIQMLDNIQRSKRNGLIIAISIGCGLAVTTRPE
                     LLDKLPYFFKEVFGSGITVGSLLALILNLVLPEDKITKKENKSDE"
     gene            complement(1311..3254)
                     /gene="ftsH"
                     /locus_tag="PWX02_002153"
     CDS             complement(1311..3254)
                     /gene="ftsH"
                     /locus_tag="PWX02_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDL1730123.1"
                     /translation="MVKNILLWVVIAIVMMTAYEGFNSNFSGSSNTVPYSTFLSDIKE
                     NRLKGVDFKQNDDTILVTKNDGATYQTVMPMYDEYLMADLAKTNAVITGQPAERRGLL
                     SQILISWFPMLLLIGFYVFYMRQMQGGGGRGAMGFGKNKAKMLTADEVKTRFSDVAGC
                     DEAKEEVGEVVEFLRDPTKFQKLGGRIPKGILMVGPPGTGKTLLAKAIAGEARVPFFT
                     MAGSDFVEMFVGVGASRVRDLFDQAKKNAPCIIFIDEIDAVGRKRGGAGFSGGHDERE
                     QTLNQMLVEMDGFEGSEGVIIIAATNRADVLDDALTRPGRFDRQVTVDLPNVKGREQI
                     LKVHLKKVPLAEGVDPMQIARGTPGYSGAQLANLVNEAALFAARKNKRVVTMEDFEEA
                     RDKINMGPERRSNTMTEKEIINTAYHEAGHVIVGYLMPEHDPLNKVTIVPRGQALGFA
                     QFLPEGDRVSETFTKLESQLSTLFAGRIAEGLIFGEDKITTGASSDIHRATQIARAMV
                     TQWGFSKELGPIFYQNEDGMGAIKGVSEESQKLIDVEMRKIIDRNYQRAKQTLEENMD
                     ILHAMKDALLKYETLDSKQIDDLMARRPVGEPSGWTDTPTKDDNATPPSGEQAADVSS
                     TLEDNPNKDNNIGDSLADTKAEH"
     gene            complement(3375..4001)
                     /gene="rlmE"
                     /locus_tag="PWX02_002154"
     CDS             complement(3375..4001)
                     /gene="rlmE"
                     /locus_tag="PWX02_002154"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HDL1730124.1"
                     /translation="MGRKRSASSSRWLAEHFKDQFVQKAHKQKLRSRAYFKLDEIQQT
                     DRLFKPGMTVVDLGAAPGGWSQYVVTQIGSKGRVIACDILDMNPIVGVDFLQGDFREE
                     SVLNALLERVGEDKVNVVMSDMAPNFSGMPSVDIPRAMYLVELALDMCRQVLATKGSF
                     VVKVFQGEGFDEYLREIRSLFSVVKVRKPEASRDRSREVYIVATGYKG"
BASE COUNT         1100 a          944 c          770 g         1288 t
ORIGIN      
        1 ttcaggcatc gtcactatta ttcaatgtcg aggtattggc ccaatcggga ttagactacc
       61 gagtgtaatg ggaactagct ttacgtttgt tgcagcagca ttagcgattg gtttcagtga
      121 aagtggcatt gccggcatta tgggagcctc tttagttggc tctctagtga tgattatcgg
      181 cagttttttt atgccttata tccgtaagct ttttccaccc gtggtaacag gtactgtcat
      241 aatgatgatt ggcctaagtt taattcctgt tgcagtagac tggtttgctg gtggtcagcg
      301 tactgatcca cactatgcaa ccccagaaaa tcttataatg gctagttttg tattaattat
      361 tgtcgttgct ttagtacaat gggggaaagg tattttttca gctgctgcta ttgtgattgg
      421 tatgatgata ggttatatcg tttcgctagc tctgggttgg gtaagtttta atggcgtgaa
      481 agaagcacaa ctttttgcgt taccacaacc tctacatttt ggcttagaat ttccaatctc
      541 cggtatcatc ggaatgtcaa ttgcttattt agttaccatt gtagaatcta gcggaaattt
      601 cttagcacta ggcaatgcaa ctaaaactga aattaccgga aaacacttac gtggaggtgt
      661 attatgtgat ggtttaggct cagcatttgc ggcaattatg tccacaactc ccttttcttc
      721 tttctcacaa aatattgggg tcatttcatt aaccggtgta gccagtcgct atgttgtagc
      781 atttacaggc gcattacttg tactagccgg tttatttcct gtatttggag cattaatcgt
      841 gtctatcccg ttacctgtgc ttggcggtgc aggcttaatg atgtttgcca tgattatcgc
      901 agccggtatc caaatgttag ataatattca acgtagcaaa cgaaacggtt taattattgc
      961 tatctctatc ggttgtgggc tggccgtaac aactcgacct gagctattgg ataagcttcc
     1021 atacttcttt aaagaagttt ttggctcagg tattacggtc ggctccttat tagcacttat
     1081 tttgaattta gttttacctg aagataaaat aaccaagaaa gaaaataaga gtgatgaata
     1141 aatcacccta aaaaaacaag gacagctcac aatacaactt tgatgctgtc ctttattccc
     1201 atagagagca tatttaaatt taggttaata aaaaaatctg cactctatat tggtgtttac
     1261 ctttagagta cagatttctt cttattctaa ctttctagca aacaattaga ttaatgttcc
     1321 gctttggtat cggctaacga atccccaatg ttattatctt tatttggatt atcctctaaa
     1381 gttgaactta catccgctgc ctgttcacca cttggtggag tggcgttatc gtctttagtt
     1441 ggggtatctg tccaacctga tggttcaccc accggacggc gtgccattaa atcatcaatt
     1501 tgcttgctgt ctaaggtttc gtatttcaat aaggcatctt tcattgcgtg taagatgtcc
     1561 atattttctt ccaaagtttg cttcgctcgt tgatagttgc ggtcaatgat cttacgcatt
     1621 tccacatcaa tcaatttttg cgactcttcc gatacgccct taattgcacc cattccatct
     1681 tcattttgat agaaaatcgg gcctaattct tttgagaagc cccattgagt aaccattgct
     1741 cgtgcaattt gggttgctcg atgaatatca gaggaagccc ctgtggtaat tttatcttca
     1801 ccaaaaatca agccctcagc aattcgccct gcaaataggg tggaaagttg gctttccaat
     1861 ttggtaaatg tttcgcttac acggtcacct tccggtaaga attgagcaaa acctaacgcc
     1921 tgaccacgag gcacaattgt cactttattc agcggatcgt gttccggcat taaatagcca
     1981 acaataacgt gtccagcttc gtggtaagca gtattgatga tctctttttc cgtcattgta
     2041 tttgaacgtc gttccggccc catattgatt ttgtcacggg cttcttcgaa atcttccatc
     2101 gttaccacac gtttattctt acgggcagca aataaggcag cttcgttcac caagtttgct
     2161 aattgagcac cagagtaacc cggcgtacca cgagcaattt gcatcgggtc aactccctct
     2221 gcaagcggta cttttttcaa gtgaactttc aaaatttgct cacgcccttt cacgttcggc
     2281 aaatcgaccg tgacttggcg gtcgaaacgc cccggacgag ttaaggcatc atctaacaca
     2341 tcggcacggt tggttgcagc gatgataatc acaccttcag agccttcaaa gccgtccatc
     2401 tcaactaaca tttggttgag ggtttgttca cgctcatcgt gtccaccgct gaaacctgca
     2461 ccaccacgtt tacgccctac cgcatcaatt tcatcaataa aaataataca aggggcattt
     2521 ttcttggctt ggtcgaataa atcacgcacc cgtgaagcac ccacacctac aaacatttcc
     2581 acgaagtcgg aacctgccat tgtaaagaat ggcactcgtg cttcacctgc aatcgctttc
     2641 gccaataagg ttttacccgt acccggaggg cctaccatca agatcccttt tggaatgcgc
     2701 ccacctaatt tttgaaattt agttgggtca cgcaaaaact caacaacttc gcctacttct
     2761 tctttggctt catcacagcc ggctacatca gaaaatctgg tcttaacttc gtctgcggtc
     2821 aacattttgg ctttattttt cccaaagccc attgcaccac gtcctccgcc accttgcatt
     2881 tgtcgcatat aaaacacata gaaaccgatt aacagcaaca ttgggaacca agaaatcaaa
     2941 atttgcgaga gcaagccacg acgctcagca ggctgaccgg taatcactgc attggttttt
     3001 gctaaatctg ccattaagta ctcatcatac atcggcatta cggtttggta ggttgcacca
     3061 tcatttttcg tcaccaaaat ggtgtcatca ttctgtttga aatcgacccc ttttaaacga
     3121 ttttctttaa tatcgcttaa aaaagtcgaa tatggcactg tatttgagct accactgaaa
     3181 ttagaattaa aaccttcata ggctgtcatc atcacaatgg caatcaccac ccaaagtaaa
     3241 atatttttaa ccatatcgtt caaggttaat cccctatctg ctgaataaaa aactgttcaa
     3301 gattactata aaagcggtcg aattttccaa tttttttgca aaatgttaat aaaaaatgac
     3361 cgcttgcttg ttttctagcc tttataacct gtcgctacaa tataaacttc tcgagaccga
     3421 tctcgagacg cctccggttt tctgactttt accacactaa agagcgagcg gatttctcga
     3481 aggtattcat caaatccttc gccttggaat accttaacca caaaactgcc tttggttgct
     3541 aatacttggc gacacatatc caatgccaat tccactaaat acattgcacg aggaatatca
     3601 accgacggca taccgctgaa attgggagcc atatccgaca tcaccacatt cactttgtct
     3661 tcgcctactc gctcaagtaa cgcattcagc accgattctt cacggaagtc gccttgtaag
     3721 aaatccacgc ctacaatcgg gttcatatct aaaatatcac aagcgattac tctgccctta
     3781 ctgccgattt gcgtcaccac atattgcgac caaccgcccg gtgcggcacc gagatccacc
     3841 accgtcattc ccggtttgaa taaacggtcg gtttgttgaa tttcatctaa cttaaaatac
     3901 gcccgtgaac gcaatttttg tttatgtgct ttttgcacaa attgatcttt aaaatgttct
     3961 gcaagccaac gtgatgaact cgcacttcgt tttctaccca tttgttcttc tatcttaatt
     4021 aattcaacga tttataataa gggttaaatt agcaaattca agagatgttc taaaatttag
     4081 ctcggcttat tatgcttaat ta
//
LOCUS       DANJWC010000175         4085 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.175, whole genome shotgun sequence.
ACCESSION   DANJWC010000175 DANJWC010000000
VERSION     DANJWC010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.175"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            61..372
                     /gene="ftsL"
                     /locus_tag="PWX02_002155"
     CDS             61..372
                     /gene="ftsL"
                     /locus_tag="PWX02_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDL1730125.1"
                     /translation="MSNERYPLHQIIIDDIFSHNKVALLLLIGVVISAVATIWITHQT
                     RLSVSEQGQLIQANQKLESQYTNLQLEENSRSSRARVDAAAKSFGLQPIKKEQEIILV
                     E"
     gene            376..2436
                     /locus_tag="PWX02_002156"
     CDS             376..2436
                     /locus_tag="PWX02_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsI"
                     /protein_id="HDL1730126.1"
                     /translation="MVKSVKLKRKQEAPVAKAEPQQAEGKKTPSFLPTRFRVVVFFML
                     LMVGGLLAQTAYIQLFDSERLIKEANNRSLRTKELQFTRGRILDRSGRFLSISVPMYS
                     LTLDPREYFDTKLRRSNESWRALAIEMETSKGKIEANVNKFIEKKNFSKEKVDFDPRS
                     ILNTKSEGYWTQLAQVTGLDYNSLIEKVRNNPSSVFLRLDNENLVRERQKFQALSKEI
                     GRSYNDIMDELYSKNRQRFLYLSRHQSEAISEYAKELDIDALVIKVESRRFYPLAEES
                     SQLIGFTDKDDMHGLEGLERSFDSLLIGKNGKQIIRKDAKGNIVENIRSEKQYDPQDV
                     MLSIDEELQSMVYGEIKKAVQENNAESGTAVLVDVQTGEILAMANAPSFNPNKRDSFK
                     PELMRNRAITDTFEPGSTVKPFTVLTALQNGVTYRDEVIGTRPFVVNGHTIKDVAPRD
                     SLTLTGILQKSSNIGVSKLALRMPANALVDTYTKVGFGKDTGLGLGEQRGTNGDRKRW
                     ADIERATLSYGYGLNVTPLQLARAYATLGSFGLYRPLSITKVDPPVIGERVLPEKTTR
                     DVVHMMESVAAKGEGGHRAAVDGYRVAIKTGTARKLEKGKYVEKYIAYTAGLAPASDP
                     RFALVVLINEPKAGNYYGGFVSAPLFSRIMGYTLKQRNIKPDNLTENTQSAVREIKLE
                     RPIN"
     gene            2445..3935
                     /gene="murE"
                     /locus_tag="PWX02_002157"
     CDS             2445..3935
                     /gene="murE"
                     /locus_tag="PWX02_002157"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDL1730127.1"
                     /translation="MKRLLPFLTELDAWVEELTELKQMTLDSRQVQAGDLFVALKGHQ
                     VDGRKFIPNAIEQGAAIILAEADEDQPEAELDSKFAKFNLDRTACCKVVSVPNLPKLL
                     SEIAGAFYHNPSHKLTLAGITGTNGKTTTAQLLAQWHNLLGGKSAVMGTIGNGLYGQV
                     QEAVNTTGSAIEIQRNLANFVELGADFCAMEVSSHGLAQFRAEALDFDLAIFTNLSRD
                     HLDYHNTMEEYAQAKFRLFNELSTKAQVINADDEIGREWLTQLPNAVAVSTDPEFDSN
                     HRFVKATAVKFTLQGASIAFKSSWGNGELHSRLIGAFNVNNLLTAFAGLLALGFDIQD
                     LIKTSPNLVGVAGRMECITAPNKPMVIVDYAHTPDALEKALQAARLHCEGELWCIFGC
                     GGDRDAGKRPLMATIAEKLTDKVIATDDNPRTEDNQKIMADIVKGFSKPQQIIHNREE
                     AIKTAIEQAKATDVILIAGKGHENYQIIGTEKLHFSDQETARKYLF"
BASE COUNT         1274 a          829 c          910 g         1072 t
ORIGIN      
        1 cgctcacgca gtgcggtatt gcggattgcc gaaaaacgct aacagtaaat agggaagaat
       61 atgtcgaacg aacgttatcc gttacatcaa atcatcattg atgatatctt tagccataac
      121 aaagtggctc ttttattgtt gattggtgta gtgatttcag cagtggctac gatttggata
      181 acccaccaga ctcgtttatc ggtgagcgaa caagggcaac tgattcaagc aaaccaaaag
      241 ttggaaagcc aatatactaa tctgcaatta gaagaaaaca gcagaagcag tcgggcaaga
      301 gttgatgccg ccgctaaatc gttcggatta cagccgatta aaaaagagca agaaattatt
      361 ttagttgaat aaaaaatggt taaatcagtt aaattaaaac gtaagcaaga agctcctgtc
      421 gcaaaagcag agcctcaaca agcagaaggt aaaaaaacac cgagtttttt acctactcgc
      481 tttagagtgg ttgtgttttt tatgctatta atggttggtg gcttactagc acaaaccgcc
      541 tatattcagc tttttgactc tgaaagatta attaaagagg ctaataaccg ctcacttcgt
      601 actaaagaac tccaatttac ccgtggacgt attttagatc gtagcgggcg ttttctctct
      661 attagtgtgc caatgtactc attaacactt gacccgagag aatactttga tactaagctg
      721 cgtcgttcta acgaaagctg gcgtgcctta gcgattgaaa tggaaacatc aaaaggtaaa
      781 attgaggcaa acgttaataa atttattgaa aagaaaaatt tttcaaaaga aaaagtagat
      841 tttgatccac gttctatttt aaatactaaa agtgaaggtt attggactca gcttgcccaa
      901 gttacaggtt tggattacaa cagtttaata gaaaaagtac ggaataaccc tagctcggtt
      961 tttttacgtt tagataacga aaatttagta agagaacgcc agaaattcca agcgttaagc
     1021 aaagaaatcg gccgttccta taatgacata atggacgagc tttatagcaa aaaccgccag
     1081 cgattccttt acttatcccg tcatcaatct gaagcgatca gcgaatatgc aaaagaacta
     1141 gatattgatg cattagtgat taaagtcgaa tcacgccgtt tttacccgct ggcagaagaa
     1201 tcttcacagt tgatcggttt tactgataaa gatgatatgc acggtttgga agggctggag
     1261 cgtagttttg attccttact tatcggcaaa aacggtaaac aaattatccg aaaagatgcc
     1321 aaaggcaata ttgtggaaaa tatccgttcg gagaaacagt acgatccgca agatgttatg
     1381 ttaagcattg atgaagaatt gcagtctatg gtgtacggtg aaatcaaaaa agcggtacag
     1441 gaaaataatg cggaatcagg cactgcggtt ttggttgatg tgcaaaccgg cgaaattttg
     1501 gcaatggcaa atgccccttc attcaaccca aacaaacggg atagttttaa acctgaatta
     1561 atgcgtaacc gtgctattac cgacactttt gaaccgggtt caacggtgaa accttttacc
     1621 gtattaaccg cattacaaaa tggggtaact tatcgtgatg aagttattgg cactcgccca
     1681 tttgtcgtca atggacacac aattaaagac gttgctccaa gggacagcct tacgcttacc
     1741 ggcatcttgc aaaaatccag taacatcggg gtcagtaaat tagctctcag aatgccggca
     1801 aatgcgttag tggatactta cacgaaagtc ggtttcggta aagacaccgg tttaggcttg
     1861 ggtgaacaac gaggtacgaa cggtgatcgc aaacgctggg cggatattga acgtgctacc
     1921 ctttcctatg gatacggttt aaatgtgacc ccattacaac ttgcccgtgc ttatgcgact
     1981 ttaggaagtt tcgggctgta tcgcccactt tcgattacca aggtcgatcc gccggtgatt
     2041 ggcgaacggg tgctaccgga aaaaaccacc cgagatgtcg tgcatatgat ggaaagcgtt
     2101 gcggctaaag gcgaaggcgg acaccgtgca gcggtggacg gctaccgtgt agccatcaaa
     2161 accggtacag cacgcaaatt agaaaaaggc aaatatgttg aaaaatatat tgcttatacc
     2221 gcagggcttg ccccggcaag cgatcctcgc tttgcattag tggtattaat caacgaacct
     2281 aaagcaggca actattatgg tggctttgtt tctgcaccac tgttctcacg cattatgggt
     2341 tacaccttaa aacaacgtaa tattaagccg gataacttaa cggaaaatac ccaaagtgcc
     2401 gtgcgtgaaa ttaaattaga acgccctatc aattaaggtt agctatgaaa cgtttactcc
     2461 cttttctgac agaacttgat gcttgggtgg aagaactcac cgagctaaaa caaatgacac
     2521 ttgatagtcg ccaagttcaa gccggtgatc tctttgttgc tttaaaagga catcaagtcg
     2581 atggcagaaa atttattccg aatgcgatag aacaaggagc agcaattatt ttggctgagg
     2641 ctgatgaaga tcagccggaa gctgagcttg attcaaaatt tgcaaaattt aatctagatc
     2701 gtaccgcttg ttgcaaggtt gtttcagtac cgaatttgcc aaaattgcta tctgaaattg
     2761 caggggcttt ttaccataac ccgtcacaca agctgacttt agcaggcatt accggtacga
     2821 acggtaaaac gactaccgct caattattgg cacaatggca taatttattg ggtggaaaat
     2881 cggcagttat gggtactatc ggcaacggtt tgtacggaca agtgcaagaa gcggtaaata
     2941 ccaccggctc cgccattgaa attcagcgta atcttgccaa ctttgttgaa cttggcgcgg
     3001 atttttgtgc gatggaagtc agctctcacg gtttggctca atttagagcg gaagccttag
     3061 attttgattt ggcgatcttt acgaatttaa gccgagatca tctcgattac cacaacacga
     3121 tggaagagta tgcccaagcc aaattccgtc tcttcaacga attaagcact aaagctcagg
     3181 tgattaacgc cgatgatgaa atcgggcgtg aatggctgac acaattaccg aatgcggttg
     3241 cggtaagtac tgatccggag tttgatagca atcatcgctt tgtcaaagcc acggcagtga
     3301 aatttaccct gcaaggggca agcattgcgt ttaaatcaag ctggggaaat ggagaattac
     3361 atagccgttt aatcggggct ttcaatgtaa ataatctgct taccgccttt gccggacttt
     3421 tagctcttgg ctttgatatt caagatctga ttaaaacttc accgaattta gttggcgtag
     3481 caggcagaat ggaatgtatc accgctccaa ataaaccaat ggtgattgtg gactatgctc
     3541 acaccccgga tgctctcgaa aaagccctac aagcggctcg tttacattgc gaaggcgaac
     3601 tttggtgcat tttcggctgt ggcggcgacc gtgacgccgg caaacgccca ttaatggcga
     3661 cgattgccga gaaattaacc gataaagtga ttgctacaga cgacaaccct cgcaccgaag
     3721 ataatcaaaa gattatggca gatattgtga aaggttttag taaaccccaa caaattatcc
     3781 acaatcgtga agaggcaatt aaaaccgcca ttgagcaagc taaagccaca gatgtgattc
     3841 taatcgcagg caaaggtcac gaaaattatc agattattgg cacagagaag ttacattttt
     3901 cggatcagga aacggcaagg aaatatctgt tttaatgtca ctttctttgc ttcgccaaag
     3961 aaagtaacca aagaaaggcg accctacttc accgctgtct tcgcttggtt tgaatttgct
     4021 taacgaaaaa attccaaact cgccgcttcg ctgctcaaac aaggaatttt tcctacaaat
     4081 tcaaa
//
LOCUS       DANJWC010000176         4070 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.176, whole genome shotgun sequence.
ACCESSION   DANJWC010000176 DANJWC010000000
VERSION     DANJWC010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.176"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(24..2651)
                     /gene="alaS"
                     /locus_tag="PWX02_002158"
     CDS             complement(24..2651)
                     /gene="alaS"
                     /locus_tag="PWX02_002158"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDL1730128.1"
                     /translation="MKTTSDIRQSFLEFFQTKGHTIVPSSSLVPENDPTLLFTNAGMN
                     QFKDVFLGLEKRPYSRATTAQRCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYF
                     KQDAIKFGWEFLTSPQWLGLPKEKLYVTVYETDDEAYDIWHKEVGVPSEHIIRIGDNK
                     GAPYASDNFWAMGDTGPCGPCTEIFYDHGPEHWGGLPGSPEEDGDRYIEVWNIVFMQF
                     NRLADGTMEKLPKPSVDTGMGLERMTAVMQHVNSNYETDIFQTLIKEVATLLNVKDLD
                     NKSLRVVADHIRACSYLIADGVLPSNEGRGYVLRRIIRRAVRHGNILGTKEAFFYKLV
                     PTLATVMGQAGEVLTQKQAHIQKTLKAEEEQFARTLERGLNLLEEALAKVENNTLSGE
                     VAFKLYDTYGFPLDLTADVCRERDIKIDEDGFNAEMTAQRERAKASSSFGTDYNNVIK
                     VDSKTCFSGYTETSLNDATVIGLFSNGKSVESIQSGENAVVILDKTPFYAEMGGQVGD
                     SGQISAEICNFEVVDTQKYGQVFGHIGQLTSGSLSVGDKVNTIVDRERRHAITLNHSA
                     THLLHSALRQVLGDHVAQKGSLVSENILRFDISQPEAITKSQLEEVERIVNAKVRENI
                     QVIIEEMDIESAKAKGAMALFGEKYGEVVRVVEMSDFSIELCGGTHVKQTGDIGLFKI
                     TSEGAVAAGVRRIEAVTGENAIAWLHTLQQAVQQSAELLKADSHSFVEKIVQLQEKAK
                     RTEKELQQLKDKLAAQAGSDLAKQATQINGVNVVIQQLENVEPKALRTMVDDLKNQLG
                     SAVVVFATTAEDKVNLIVGVTKDLTDKVNAGQLVGAMAEKVGGKGGGRPDMAMAGGSE
                     PQHLLQALSLAQEWITAKL"
     gene            complement(2846..3271)
                     /locus_tag="PWX02_002159"
     CDS             complement(2846..3271)
                     /locus_tag="PWX02_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL1730129.1"
                     /translation="MYKHILVAVDLSEESLVLVRKGAALAEKCGAKLSLIHVDVNFSD
                     LYTGLIDINMSSVQDSVVEETNTALAELASKVNYPVSECLNGTGDFSQVLEEAVEKHN
                     VDLLITGHHQDFWSKFMSSTRQVMNNITVDMLVVPLADE"
     gene            3502..>4070
                     /gene="fnr"
                     /locus_tag="PWX02_002160"
     CDS             3502..>4070
                     /gene="fnr"
                     /locus_tag="PWX02_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HDL1730130.1"
                     /translation="MKIVSEPKTSGRHTCTIHCQNCSISQLCLPFTLNETELTQLDNI
                     IERKKPIQKSQVIFKSGDELRSLYAIRSGTLKAYTLSESGEEQITGFHLPGDLIGFDA
                     ITNMQHESYAQALETSMICEIPFDILDDLAGKMPKIRHQIMRLMSNEIKSDQEMILLL
                     SKMNAEEKLAAFLYNLSQRYSARGFSVKE"
BASE COUNT         1181 a          872 c          690 g         1327 t
ORIGIN      
        1 ttcagctcgc cttgttatta tttttaaagt tttgctgtaa tccattcttg agcaagtgac
       61 aatgcctgca ataaatgttg tggttctgag cctccggcca ttgccatatc aggacgtccg
      121 ccccctttac ctcccacttt ttcagccatt gcaccaacta attgacccgc attgacttta
      181 tcggttaaat ctttggtcac acctacaatc aaattgactt tatcttccgc cgtagtcgca
      241 aatacaacaa ctgcactgcc taattgattt tttaggtcat caaccatcgt tctcaatgct
      301 tttggttcaa cattctctaa ttgttgaatc actacattca cgccattgat ttgggtagct
      361 tgtttggcta aatctgaacc tgcttgtgct gcaagtttat cttttagctg ttgaagctct
      421 ttttctgtac gtttagcctt ctcttgtaat tgaacaattt tctcaacgaa tgaatgactg
      481 tctgctttta acaattcagc actttgttga acggcttgtt gtaaggtatg caaccaagca
      541 atcgcatttt caccagtgac agcctctata cggcgtacac ctgctgctac cgcaccttca
      601 gaggtaattt tgaataagcc aatatcaccg gtttgtttaa cgtgtgtgcc gccacaaagt
      661 tcaatcgaga aatctgacat ttccactaca cgcactactt cgccatattt ttcaccaaat
      721 aacgccattg cacctttcgc ttttgcagat tcaatatcca tttcttcaat gattacctga
      781 atattttcac gcactttagc attcacgata cgttcaactt cttcaagttg tgatttagta
      841 attgcttccg gctgagaaat atcaaaacgt aaaatatttt ctgacactaa tgagcctttt
      901 tgagcaacgt ggtcgcctag cacttggcgt aacgcagaat gcagtaagtg ggtagcactg
      961 tggtttaatg taatagcatg acgacgctct ctatctacga tcgtattaac tttgtcgcca
     1021 acagaaagtg agccggaagt taattgccca atatgcccga atacttgacc atatttttgc
     1081 gtatctacta cctcaaaatt gcaaatttct gcggaaattt gaccgctatc tcccacttgt
     1141 ccgcccattt ctgcatagaa tggggtttta tctaaaatca ctaccgcatt ttcacccgat
     1201 tgaatagact caaccgattt tccattacta aataacccaa ttacagtagc atcatttaag
     1261 ctggtttcag tatatccact aaagcatgtt ttactgtcca ctttaatcac attgttataa
     1321 tctgtgccaa agctactgct ggctttcgca cgctcacgtt gtgcggtcat ttctgcattg
     1381 aagccgtctt cgtcaatttt aatatcacgt tcacggcaaa catctgcggt taaatctaac
     1441 gggaagccat aagtatcata aagtttgaac gcaacttcgc cagataaggt gttattttca
     1501 accttcgcta atgcttcttc taataaattc aatccacgct caagagtacg ggcaaattgc
     1561 tcttcttctg ctttgagtgt cttttgaata tgcgcttgtt tttgggtcag cacctcacca
     1621 gcttgcccca ttaccgttgc aagtgttggc accaatttat agaaaaaggc ttcttttgta
     1681 cccaaaatat taccgtgacg aaccgcacgg cggataatac ggcgtaatac atagcctctt
     1741 ccttcatttg aaggtaaaac accatcggca atcagatagg aacaagcacg aatatggtct
     1801 gccactacac gtaaagattt attatctaaa tctttcacat taagcaaagt agcgacttct
     1861 ttaattaaag tttggaaaat atccgtttcg tagttggagt ttacgtgttg cattaccgca
     1921 gtcatacgtt ctaaccccat ccctgtatct acagaaggct taggcagttt ttccatcgta
     1981 ccgtctgcta aacgattgaa ctgcataaat acaatattcc atacctcaat atagcggtcg
     2041 ccatcttctt ctgggctacc cggtaagcca ccccaatgtt caggcccgtg atcatagaaa
     2101 atctcggtac aagggccaca agggccggta tcgcccatcg cccagaagtt atctgaagca
     2161 taaggtgcgc ctttattatc gccaatgcga ataatatgtt ccgatggtac acctacttct
     2221 ttatgccaaa tatcgtaggc ttcatcatca gtttcataca cagttacata taatttttct
     2281 tttggtaagc caagccattg tggagaagtt aaaaactccc aaccgaattt gattgcatct
     2341 tgcttgaagt aatcaccaaa gctgaaattc cccatcattt caaaaaaagt atgatggcga
     2401 gcggtataac ctacgttttc taaatcattg tgtttaccac ctgcacgtac acaacgttgt
     2461 gccgtagtcg ctctgctata agggcgtttt tctaacccaa gaaaaacatc tttgaattgg
     2521 ttcatgcccg cattggtaaa taataatgtc ggatcattct cgggaacaag agagctgctt
     2581 ggaacaatcg tatgtccttt agtttggaaa aactctagaa aagattgtct gatatctgaa
     2641 gttgttttca ttaaaaatca ctctttttgt aaatagaagt taaacattta gttatcttgc
     2701 tattttacac attttttatg ctattggggg agctatatgc tacttatttt ttcaattaaa
     2761 taatttttgt aaattttccc aaaaaaataa ccgcttgtaa gacagataaa ctgtctcaag
     2821 cggttatttt tcattcattt tcttgctatt catctgctaa tggcacaacc agcatatcaa
     2881 cagtgatatt attcatgact tgacgagttg aagacataaa tttactccag aagtcctgat
     2941 gatggccagt gatgagtaaa tcgacattgt gtttttctac ggcctcttcc aaaacttgac
     3001 taaaatctcc cgttccgttt aaacattcag atacaggata atttactttt gaggcaagtt
     3061 cagccagtgc cgtatttgtt tcttcaacaa cactgtcttg taccgaagac atattaatat
     3121 caattaatcc tgtatagaga tccgaaaaat taacatcaac gtgaatgagg gaaagtttag
     3181 ctccgcattt ttctgctaac gcagccccct tacgcactaa aacaagactt tcttcagaaa
     3241 gatccactgc aactaagata tgtttataca taatatgctc cttgcttgtg agagttgttt
     3301 actttgtgta taaacatact aacacactct ttttgataaa aacttgaagt agatctcaaa
     3361 aataaaaatt tgattatatt tttcatacct ctttgtaagt atttttattt ttccttggta
     3421 tactttgctt catgtaaatt taatggtaaa ctttgttcta aatcaaattt aggaatactt
     3481 ttctaattta aggttgaatg gatgaaaatt gtatcagaac ctaaaacaag cggacgtcat
     3541 acttgcacaa ttcattgtca aaattgcagt attagccaac tatgcttacc ttttactctt
     3601 aatgaaacgg aattaactca actagataat attattgagc gtaaaaaacc gattcaaaaa
     3661 tcgcaagtta tttttaagtc aggtgatgaa ctgcgttcgc tttatgctat ccgttctggg
     3721 acattaaaag cctatacctt aagtgaaagt ggtgaagagc aaattaccgg tttccattta
     3781 cctggcgatc tgattggctt tgatgcaatt accaatatgc agcacgaaag ctatgctcaa
     3841 gcactcgaaa cctcaatgat ttgtgagatt ccattcgata ttttagatga tcttgccggc
     3901 aaaatgccaa aaattcgcca tcaaattatg cgtttgatga gtaatgaaat taaaagcgat
     3961 caagaaatga ttctattgct ctcaaaaatg aatgcggaag aaaaactagc tgcatttttg
     4021 tataatcttt cccaacgtta ctcagcccgt ggtttctctg taaaagagtt
//
LOCUS       DANJWC010000177         4032 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.177, whole genome shotgun sequence.
ACCESSION   DANJWC010000177 DANJWC010000000
VERSION     DANJWC010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.177"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     misc_feature    complement(98..243)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            complement(420..1430)
                     /gene="mglC"
                     /locus_tag="PWX02_002161"
     CDS             complement(420..1430)
                     /gene="mglC"
                     /locus_tag="PWX02_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018355241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HDL1730131.1"
                     /translation="MSALQQNKTLDFFKQNAIYFVLLILLIVIIVQDPSFLSLRNFSN
                     ILSQSSVRLIIALGVAGLIVTQGTDLSAGRQVGLAAVISATLLQSMENMNKVFPNLSE
                     IPIPVVVLTVCAIGAVIGLFNGFVVAYLNVTPFIATMGTMIIVYGINSLYYDAVGSSP
                     IAGFSDDFSTFAQGFFRFGDFRLSYITIYAVIATIIMWTLWNKTRFGKNVFAIGGNPE
                     AARVSGVNVPLNLMGIYMLSGIFYAFGGMLEAGRIGSATNNLGFMYEMDAIAACVVGG
                     VSFAGGVGTIIGVVTGVLIFTVINYGLTYIGVNPYWQYIIKGGIIILAVAIDSLKYAK
                     KK"
     gene            complement(1444..2943)
                     /gene="mglA"
                     /locus_tag="PWX02_002162"
     CDS             complement(1444..2943)
                     /gene="mglA"
                     /locus_tag="PWX02_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005709009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HDL1730132.1"
                     /translation="MTGEVLLTMTNVSKSFPGVKALDNANLTVKSHSVHALMGENGAG
                     KSTLLKCLFGIYAKDEGDILFLGKPVNFKTSKEALENGISMVHQELNLVKQVTVMDNL
                     WLGRYPLKGPFVDHDKMYRDTKAIFDELDIDVDPRDKVAKLSVSQMQMIEIAKAFSYN
                     AKIVIMDEPTSSLSEKEVEHLFKIIQKLKDRGCGIIYISHKMDEIFKICDEITILRDG
                     KWVNTVAIKDTSMDQVVSMMVGRELTQRFPEKTNLPKEVILEVEHLTAKNQPSIQDVS
                     FSLRRGEILGIAGLVGAKRTDIVEIIFGVREKAGGTIRLNDKEINNRNAFDAINNGFA
                     LVTEERRSTGIYANLSIEFNSLISNMKSYLGKFGLLSNKKMKSDTQWVIDAMNVKTPS
                     HQTHIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDIGAKFEIYQLILELANKDK
                     GIIMISSEMPELLGVTDRILVMSNGKVAGIVETAHTSQEEILQLAAKYL"
     gene            complement(3015..4004)
                     /gene="mglB"
                     /locus_tag="PWX02_002163"
     CDS             complement(3015..4004)
                     /gene="mglB"
                     /locus_tag="PWX02_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDL1730133.1"
                     /translation="MKKAVLSAVAMAVALGATISTAQAENRIGVTIYKYDDNFMALMR
                     KEIEKEGKTQKVELLMNDSQNTQSIQNDQVDVLLSKGVKALAINLVDPSAYKTIIQKA
                     KSEGVPVVFFNKDPGAAAIDSYPKAYYVGTEPKESGIIQGELIAKQWKAHPELDLNKD
                     GKIQFALLKGEPGHPDAEARTKYVVESLKAQGIDSEQIFMDAALWDAAQAKDKVDAWL
                     SSGKAKDIEVIISNNDGMALGALEATKAHGRKLPIFGVDALPEALQLIKKGELAGTVL
                     NDGVNQGKAVVQLSANLAGGKDAVAGTQWKLENRVVRIPYVGVDKDNLSEFLK"
BASE COUNT         1158 a          884 c          724 g         1266 t
ORIGIN      
        1 agatttaggt gctgcgaaag cagaacctgt aaatgctaaa gaaataagtg caagttgtgt
       61 cagcttgttc attgtcatat ccttaaatgt taagcaataa aaaacccttt caaaagttgc
      121 cttccgaaag ggttagaatt gggtttgcga ttttggattg cgtttgccat ttcaggtttt
      181 cggaagatat cttccgaaca cgccaaatgc ccgaaagatt aatcggtcaa atggtagtga
      241 tgatgtttaa cgcaatagct cataatattc tccaaaattg aatgtttagg aatttacgct
      301 ttttaaaaat caattgcaaa cgttttcttt aaaattttgt aaaaaattca acatttttaa
      361 ccgcttgtat cgtacctagg agcaaatggc gatttgctcc taggtttcca agcaataggc
      421 tattttttct tcgcatattt aagtgagtca atcgctaccg ccaagataat gatgccacct
      481 ttaataatat attgccagta agggtttact ccaatatagg ttaagccgta gttgataacg
      541 gtgaagatta acacccctgt tactacaccg atgatagtac ccacaccacc ggcaaaggat
      601 accccaccta ccacacaagc agcgattgcg tccatttcat acataaagcc caagttgtta
      661 gtcgcacttc cgatacgtcc tgcttctaac ataccgccga aggcatagaa gatgcctgaa
      721 agcatataaa tacccatcag gttaagcggt acgtttacac cggaaactcg tgctgcttca
      781 gggttgccgc caatagcaaa tacgttttta ccgaagcggg ttttattcca taacgtccac
      841 ataataatgg tagcaatcac ggcataaata gtgatgtagg atagcctaaa gtcgccaaag
      901 cggaagaagc cttgtgcaaa ggttgagaaa tcatcgctaa agcctgcaat tggcgatgag
      961 cctactgcat cgtagtaaag cgagttgata ccatacacaa taatcattgt tcccatcgtg
     1021 gcaataaacg gtgttacgtt gaggtaagcc accacaaagc cgttaaataa accaatgaca
     1081 gcaccgatgg cacacacggt taatacaacc acaggaatcg ggatttccga gaggtttggg
     1141 aataccttgt tcatattttc cattgattgc aacaaggttg ccgagattac cgcagcaagc
     1201 cctacctgac gaccggcaga taagtcggtt ccttgtgtga caatcaagcc tgcaacccct
     1261 aatgcaataa tcagacgcac agaagattgc gataaaatat tactgaagtt acgcaagctc
     1321 aagaaacttg gatcttgcac aataatcaca attaataaga ttaaaagtac aaagtaaatc
     1381 gcattttgtt taaaaaaatc gagcgtttta ttttgttgta aggcagacat aattcactcc
     1441 ttgttataag tattttgctg cgagttgcaa gatttcttct tgcgaggtgt gagcggtttc
     1501 gacaatacct gcaactttcc cgttactcat cactaaaatt cggtctgtga ccccaagcaa
     1561 ttccggcatt tccgatgaaa tcataatgat gcctttatct ttattcgcca gctctaaaat
     1621 caactggtaa atttcaaatt ttgccccgat atcgatacca cgggtcggct cgtcaagcat
     1681 caagatttca ggctgggtta acaaccaacg cccgataatc actttttgtt ggttaccgcc
     1741 ggaaagtgag ccgatatggg tttggtgtga tggcgttttt acattcatcg catcaattac
     1801 ccattgggta tcgcttttca tttttttatt gctcagtaag ccgaacttac ctagataaga
     1861 cttcatattt gaaatcagag agttaaactc aatgcttaag ttggcataaa tccctgttga
     1921 acggcgttct tctgtaacca atgcaaagcc gttattaatc gcatcaaaag cgttgcggtt
     1981 attgatttct ttatcgttta gtcggatagt gccgcctgct ttctcccgta caccgaaaat
     2041 gatttcaaca atatcagtcc gttttgctcc aactaagccg gcgatgccta aaatttcccc
     2101 tctgcgtaag ctaaagctga catcttgaat agagggttga ttttttgccg ttaaatgctc
     2161 aacttcaaga atcacttctt tcggcagatt ggttttttca gggaaacgct gggtcagctc
     2221 acgccctacc atcattgaaa ccacttgatc cattgaggta tctttaattg caacggtgtt
     2281 tacccattta ccgtcacgca aaatggtaat ttcatcacaa attttaaaaa tttcgtccat
     2341 tttgtgtgaa atatagataa tgccacagcc tctgtctttc agtttttgga taattttaaa
     2401 taaatgctcg acttcttttt ctgaaagtga cgaagttggt tcgtccataa tcacaatttt
     2461 ggcattgtaa gagaaagctt ttgcaatctc gatcatctgc atttgtgaaa ccgataattt
     2521 ggctacttta tcacgtggat caacatcaat atctaattca tcaaaaatcg ccttagtatc
     2581 acgatacatt ttatcgtgat ctacaaaagg cccttttagt gggtaacgac ctagccataa
     2641 gttatccatt accgtcactt gttttactag gttcaactct tggtgaacca ttgagatccc
     2701 attttctaac gcctcttttg aggtcttaaa attcaccggc ttgccaagaa agagaatatc
     2761 tccctcatct ttagcataaa tcccaaataa gcatttcagt aacgttgatt tacctgctcc
     2821 attctctccc attaaggcgt gaacactatg ggatttcacc gttaagttag cattatctaa
     2881 ggcttttacc ccgggaaatg acttgctaac atttgtcatt gtgagtaaaa cttcacctgt
     2941 catatctcac ccctgattac atcgaaaaat cgaaaaaaag cacgggtatc catcacccgt
     3001 gctaaaagcg tgaattattt taaaaattca gataagttgt ctttatcgac accaacgtaa
     3061 ggaatacgca ccacacggtt ttccaatttc cattgtgtac cagcaactgc atctttaccg
     3121 cctgctaagt tagcagaaag ctgaacaacc gctttacctt ggtttacacc atcatttaat
     3181 accgtaccgg ctaattcacc ttttttgatt aattgaagtg cttctggcaa tgcatctaca
     3241 ccgaagattg gtaattttct accgtgagct ttggttgctt ctaatgcacc taacgccata
     3301 ccatcattat tagaaataat tacttcgatg tctttcgctt taccacttga taaccaagca
     3361 tccactttat ctttagcttg ggctgcatcc cataatgctg cgtccataaa gatttgctca
     3421 ctgtcgatac cttgtgcttt taagctttct accacatatt tagtacgtgc ttccgcatca
     3481 gggtgacccg gctcaccttt taataaggca aattggattt taccgtcttt attcaagtct
     3541 aattcaggat gggctttcca ctgttttgca attaactcac cttgaataat gcctgactct
     3601 ttcggctcag tacctacata ataagctttc ggatagctat caatcgcagc tgcacccggg
     3661 tctttattga agaacaccac aggcacacct tcactttttg ctttttgaat aattgtttta
     3721 taagctgatg gatccactaa gttaattgct aatgctttaa cccctttaga aagtaataca
     3781 tcaacttggt cgttttgaat agattgcgta ttttgcgagt cattcattaa taactcgact
     3841 ttttgtgttt taccctcttt ttcgatttct ttacgcatta atgccataaa gttatcgtca
     3901 tatttataaa ttgttacacc aatacggttt tcagcttgag ctgtactgat cgttgcacct
     3961 aatgcgactg ccattgccac tgcacttaat actgctttct tcataggaga tcacctcttt
     4021 tatgtgttta ta
//
LOCUS       DANJWC010000178         4031 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.178, whole genome shotgun sequence.
ACCESSION   DANJWC010000178 DANJWC010000000
VERSION     DANJWC010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.178"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..>4031
                     /locus_tag="PWX02_002164"
     CDS             <1..>4031
                     /locus_tag="PWX02_002164"
                     /inference="COORDINATES: protein motif:HMM:NF017476.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730134.1"
                     /translation="ESVTVGDNLAVQQDKNNNTGGKNYHVTLNNTLNLGNDGSVTIGD
                     TVINQNGLTINNGGPSVTTIGINAGNKVITNVADGQNDTDAVNLRQLKAVQTKVEKGT
                     NVASVTANKDATGVTTYTINADGTTASADEASKDFLTVTTGEKNQDNVTDYKVGLTKK
                     ATDALAKAETAIQEFTTSVNGQTVETINKDNKDVGFVNGKGTIARNDNSNITFDVDVD
                     DNTIKVGEDGKLTVNKEAVDTNTYVTGGTVVYADNGDATTTLNKNDGSSVTVTGIKNN
                     FVNKAETSTDGKTATLTRNDGGTVNIDLTNTVNTAVNEATEKGFKISADKGDEDHVKL
                     GETVKYTSTDKNIITTVTNNQIDFALNSTLAIGGKDGKSGIGLDGTNGTIGLTGPAGK
                     DGKSATANITVKNGQPGVDGQDGETKTRIVYDGEEVATLNDGLKFGANTGDVHNAKLN
                     TQVNVKGAEDNTEWNKFDAGQNIMTQISGNNITVALAKNVNLTEDGSVTIGGTNITNE
                     GITINNGPKITNKEGSVSVANQDGSPTKITNVKAGDVNNTSTDAVNGSQLYTVQEIAN
                     AGWNLTINEGQNSSNVKPKETVDLNNKDGNIQITKDAHNVTFALNNELTIGGPGKNGA
                     DGKDGSIGVKGADGSTGVALNGKDGSIGINGKDGANANITVKNGKPGVDGVDGTTKTR
                     IVYETKDGPEEVATLNDGLKFVGDDDQVITKKLNQTLNVTGGADLTKLADNNIGVVNT
                     NNGLVVKLSKDVNLTKDGSVTIGNTTLNDSGLTIANGPSVTNTGISAGNKQITNVSSG
                     LTNANGEKTDLANATTTNAVNVGDLKDTVNNLTNATTGGFGLTGDDKNTEVKQDLGKT
                     IQVKGKDGVTVTANVAEKALEIVLAGDVSVSGKDGKDGSIGVKGTDGKDGVTINGNGT
                     VVVGKDGAPGKIGINGKDGANATMTVEKGAPGLDGAKGKDGKDGTQTTRIVYTNETGG
                     KEEVATLNDGLRFTGNNEVENKHKLNSLVTIIGEGVNKEQSGAFQSAAGNINVVADGN
                     GNLTVKLAKNLNLTDSGSVTIGGTNITNEGITINNGPKITNDGDNVKVGDVNGAPVKI
                     TNVKEGDLSETSKDAVNGSQLYATNQNVTNLSNKVAQGFNITADNGTADNVQLGETIT
                     YTSSDKNIVTTVSDNKIDFKLSAEAVDKFTVKYFSVNSTVAENRLNDGAKGVNSIAIG
                     PNATSVDQGAIALGTNSNANGNAAAALGVNSKAEGIQATAVGSQANATANGTTAIGRE
                     TTASAGEATAVGSNANATSEKATALGVGSKATDKAALAVGTNATASAQSAVAVGTQSV
                     ADKQFAVAVGMGAQATLENSVAL"
BASE COUNT         1453 a          611 c          890 g         1077 t
ORIGIN      
        1 aggaaagcgt aactgtaggt gacaacttag cagttcaaca agataaaaat aataatacgg
       61 gtggtaagaa ctatcatgta acgttgaata acactttaaa cctgggtaat gatggttctg
      121 taacaattgg tgatacagtt attaatcaaa atggtttaac tattaacaat ggtggaccga
      181 gtgttacaac aataggtatt aatgctggta acaaagtaat tactaacgta gcagatggcc
      241 agaatgacac agatgcagta aatctccgcc agttaaaagc tgtgcaaact aaagtagaaa
      301 aaggtacaaa tgttgcatct gtcactgcaa ataaagatgc aacaggtgta acaacttata
      361 ctattaatgc tgatggtaca actgcgagtg cagatgaagc ttctaaagat ttcttaactg
      421 ttactacggg tgagaaaaac caagataatg taacagatta caaagttggc ttaactaaga
      481 aagcaacgga tgctttagcg aaggctgaaa ctgcgattca agagtttact acttcagtaa
      541 atggtcaaac tgttgaaaca attaacaaag acaacaaaga tgttggtttt gtaaatggta
      601 agggcacaat tgctcgtaat gataatagta acattacctt tgatgtagat gttgatgata
      661 atactattaa agtaggtgag gatggtaaat taactgttaa caaagaggca gttgatacta
      721 atacttatgt tactggtggc acagtagtgt atgctgataa tggcgatgca acaacaacgt
      781 taaacaaaaa tgatggttca tctgttactg taacaggcat taaaaataac tttgttaata
      841 aagcagaaac ctctacagat ggtaaaactg caactttaac tcgtaatgat ggtggtacag
      901 ttaatattga cctaactaat acggtaaaca cagctgtaaa tgaagcaaca gagaaaggct
      961 ttaagatttc agctgataaa ggtgatgaag atcacgttaa gctaggcgaa acagttaaat
     1021 atacctctac tgataaaaac attattacga ccgtaacgaa taaccaaatt gattttgcgt
     1081 taaattcaac tttagctatt ggtggcaaag atggtaaatc aggtattggt ttagatggta
     1141 ctaacggtac aatcggttta actggcccag cgggtaaaga cggtaaatct gcaacagcaa
     1201 atattactgt taaaaatggt caaccaggtg ttgatggtca agatggggag accaaaactc
     1261 gtattgttta tgatggtgaa gaagttgcaa cccttaacga tggcttaaaa tttggcgcta
     1321 acacaggtga tgttcacaat gcaaaattaa atacgcaagt gaatgtaaaa ggtgctgaag
     1381 acaataccga gtggaataag tttgatgcag gtcagaacat catgacccaa atcagcggta
     1441 ataatattac tgttgcattg gcgaagaatg tgaacttgac tgaagatggc tctgtaacta
     1501 ttggtggcac aaacattact aatgaaggta ttaccattaa taatggcccg aaaatcacta
     1561 ataaagaagg tagtgtaagt gttgctaatc aagatggaag tccaacaaaa attactaacg
     1621 tgaaagcggg tgatgtaaac aatacttcta cagatgctgt taatggaagc cagttatata
     1681 ctgtacaaga aattgcaaat gcaggttgga atttaactat taatgaagga cagaatagca
     1741 gtaatgttaa gcctaaagag acggttgatc taaataataa agatggcaac attcaaatta
     1801 ctaaagatgc ccataatgta acatttgcat taaataatga gttaactatt ggtggaccag
     1861 gtaaaaatgg tgctgatggt aaagacggct ctatcggtgt taaaggtgct gatggttcaa
     1921 ctggtgttgc tttaaatggt aaagatggtt caattggcat taacggtaaa gatggtgcta
     1981 atgcaaatat tactgttaaa aatggtaaac ctggtgttga tggcgtagat ggtacaacta
     2041 aaacacgtat tgtttatgaa accaaagatg gtccggaaga agttgcaaca cttaacgatg
     2101 gtttaaaatt cgttggtgat gatgatcaag ttattactaa gaaacttaac caaactttaa
     2161 atgtaactgg tggtgctgat ttaaccaaat tggctgataa caatattggt gtagtaaata
     2221 ctaataatgg attagttgtt aaattatcta aagatgtaaa cttaactaaa gatggttcgg
     2281 taactattgg taatacaact ttaaatgatt caggattaac tattgctaat ggtccaagtg
     2341 tgactaacac aggtattagc gctggtaaca aacagatcac caatgtatca agcggtttaa
     2401 ctaatgcaaa tggcgaaaaa actgatcttg ccaatgcaac gacaaccaat gcggttaacg
     2461 taggcgattt aaaagataca gttaataacc taactaatgc aacaacgggt ggctttggtt
     2521 taactggcga tgataagaat actgaagtta agcaagactt aggtaaaacg attcaagtta
     2581 aaggcaaaga tggtgtaacc gtaaccgcaa atgttgctga aaaagcatta gaaattgtat
     2641 tagccggtga tgtatctgta agtggtaaag acggaaaaga cggctctatc ggtgttaaag
     2701 gtacagatgg caaagacggt gtaactatca atggtaacgg cactgttgta gtcggcaaag
     2761 atggtgctcc aggtaaaatt ggcatcaacg gtaaagatgg tgcaaatgca acaatgactg
     2821 ttgagaaagg cgctcctgga ttagatggtg ctaaaggcaa ggatggtaaa gatggtactc
     2881 aaactactcg tatcgtttac acaaacgaaa caggtggtaa agaagaagtt gcaaccttaa
     2941 acgatggttt acgttttacc ggtaataacg aagttgagaa caaacataag ttaaacagct
     3001 tagttactat tattggtgag ggtgtaaata aagaacagtc aggcgctttc caatcagcag
     3061 caggcaatat taatgttgtt gcagatggca atggtaattt aactgttaaa ttagcgaaaa
     3121 acctaaactt aactgattca ggttctgtaa ccattggtgg cacaaacatt actaatgaag
     3181 gtattaccat taacaatggt ccgaaaatca caaatgatgg cgataatgtt aaagttggtg
     3241 atgtaaatgg tgcgccagtt aaaatcacga atgttaaaga aggtgattta agcgaaactt
     3301 ctaaagacgc agttaacggt tcacagcttt atgcaactaa ccaaaatgta accaacttaa
     3361 gcaataaagt agctcaaggc ttcaatatta ctgctgataa tggtacggct gacaatgttc
     3421 aattaggtga aactattacc tatacttcat ctgataaaaa tatcgttaca acggtaagcg
     3481 acaataagat cgattttaaa ctgagtgctg aagctgtaga taaatttacg gttaaatact
     3541 tctctgtaaa ttcaacggtt gcagaaaaca gattaaatga tggtgctaaa ggtgttaact
     3601 ctattgcaat tggtccgaat gcaacctctg tagatcaagg tgctattgca ctgggtacaa
     3661 actcaaatgc aaatggtaat gctgcagcgg ctttaggtgt caactcaaaa gctgaaggta
     3721 ttcaagcaac tgcggtaggt tctcaagcaa atgcaactgc aaatggtact actgcgattg
     3781 gtcgtgaaac aacagcaagt gcaggtgagg caacagctgt gggttctaac gcaaatgcaa
     3841 catcagagaa agcaactgct ttaggtgtag gctcaaaagc gacagataaa gcagcattag
     3901 ctgtaggtac aaatgcaact gcaagcgcac agtctgcagt agctgtaggt actcaatcgg
     3961 tagctgataa acagttcgct gttgcagtag gtatgggggc tcaagcaacc ttagaaaact
     4021 ctgtagcatt a
//
LOCUS       DANJWC010000179         4028 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.179, whole genome shotgun sequence.
ACCESSION   DANJWC010000179 DANJWC010000000
VERSION     DANJWC010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.179"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            130..687
                     /locus_tag="PWX02_002165"
     CDS             130..687
                     /locus_tag="PWX02_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDL1730135.1"
                     /translation="MLNYLNSALATLAVQLGERKPVGYSLKPWAKNIAARIRESSVAD
                     CCQVVDYLVAKWGRDGKMREYLCPKTIFRQSNFADYLPKSTAWASNGKPVCVNGKWVA
                     TAELEKRLIMPTVEEVKVLFQKSLSGNPFKSLDFSNKRNLVMYHATINTKNKRPLERD
                     LPMVISQEIKNAVECIDRLKVPTFS"
     gene            698..1270
                     /locus_tag="PWX02_002166"
     CDS             698..1270
                     /locus_tag="PWX02_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HDL1730136.1"
                     /translation="MTDFDRDSYQTPKYLRNWLNHRYAWFHIDGCSNGKNALYSYWIG
                     KAAEGLDEDTLSGQIAEDFLADNLLDVILECVADWGELLRIFVNPPYSNPLPFVQRAA
                     ELKKAGHIVVMLLPADKSTRWYQVIQENASEVIDIIGGRINFINPLTGEEVKGNNKGS
                     MVAVFDPFVEGIVTRQMPLDKIKELGGYEG"
     gene            1291..1752
                     /locus_tag="PWX02_002167"
     CDS             1291..1752
                     /locus_tag="PWX02_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HDL1730137.1"
                     /translation="MAKYQMMKLPGGVLSPASEADEESLKALKNHELYDVEINLKINP
                     VLHRKMFSFFKFCFEHWCAENGLQKFSNEKTQFDEFRKDLLVQAGHCEMVFSLSNPNE
                     FKLVPKSISYKELEDDVKRRELYVAITNAAMATVFKGCNDENIINQLYSYF"
     gene            1825..2394
                     /locus_tag="PWX02_002168"
     CDS             1825..2394
                     /locus_tag="PWX02_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HDL1730138.1"
                     /translation="MANKPPKQHKCKECGGYYIKFQSTQQVCSVKCAMAMGKRKTETK
                     RKQADKAERKERKQRLEKLKSRSAWLKDLQNIFNKFIRLRDKDLPCISCGRHHQGQWH
                     AGHYKTVGGNPELRFNEDNVHRQCSVCNNHLHGNIVNYRVNLIEKIGLERVEFLERKD
                     HPPLKLTIEQIKDLIKVYKAKCKELERVT"
     gene            2384..2857
                     /locus_tag="PWX02_002169"
     CDS             2384..2857
                     /locus_tag="PWX02_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730139.1"
                     /translation="MLPRKIKNKWLEPEKQKWIEELLRLWGAWEFGGLDLESRVNMIY
                     RLMKSAEGVKSLSSREVCNDSLGTLINEIFTVVTKRDVMLADILKQKYWFGRSERQIA
                     IYYQLRDSEGRKERRWKEIIQEKCKKAERIIADILESKISSSHSANKLKKYQFNP"
     gene            2993..3352
                     /locus_tag="PWX02_002170"
     CDS             2993..3352
                     /locus_tag="PWX02_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HDL1730140.1"
                     /translation="MKMPDKDPNVWLVISAYIQQNYNAITGFVMAFFMSMLRAWFLQQ
                     KSTYRQRFLDGAICGALTLSCMSLLTHFGVGESLSTFAGGMIGFVGAEKIREFLFALI
                     RKKIEVNDVGFNRKSDE"
     gene            3367..3963
                     /locus_tag="PWX02_002171"
     CDS             3367..3963
                     /locus_tag="PWX02_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HDL1730141.1"
                     /translation="MLISERKFKQVFPNAIDGIYAGIAKHIEKAGCKTKEQQAMFLAQ
                     CGVECAGYKTFEENLNYSATALLATFPKYFTQHNVVLYARDKRAIANRVYANRMGNGN
                     EQSGDGWKYRGRGLIQITGKANYIEFAKWEGLPSLVTSPDQITNNLALCVQVAVWYWQ
                     SRKLANYGSDVLKVTKLINGGTHGLSQRTNYYQRLMAA"
BASE COUNT         1270 a          678 c          988 g         1092 t
ORIGIN      
        1 caaactcgga aaaatttccg agaaagctcg gaaaaaaatc cgacagataa taatactaat
       61 tataaaaata ctaatgatca tacttccccc cttaatcccc caacggggga gcctgctcct
      121 gcagaagttg tgttgaatta tctcaattcg gctttggcaa cattggcggt acagcttggc
      181 gagcgtaagc ctgtggggta ttcgctcaag ccgtgggcaa aaaacattgc tgcacggatt
      241 cgggaaagtt cggtggcgga ctgttgccaa gtggtggatt accttgtcgc caaatggggg
      301 cgagacggca aaatgcgtga atacctctgc ccgaaaacga ttttccgcca atcgaatttt
      361 gcggattatt tgccaaaatc gaccgcttgg gcgagcaatg gcaagccggt ttgcgtaaat
      421 ggcaagtggg tagcaacagc agagctagaa aaacgcttga ttatgccaac ggtggaggaa
      481 gttaaagtct tgtttcagaa atcactaagt ggcaatccgt ttaagtcgtt ggatttcagc
      541 aataaacgca atttagtgat gtaccacgcc accataaaca ccaaaaacaa gcgaccactt
      601 gagcgagatt tgccgatggt tatcagccaa gaaattaaaa acgccgttga gtgtatcgac
      661 cggctaaaag taccgacatt ttcgtaagga ttagacaatg acagattttg acagagatag
      721 ttaccaaacg ccaaagtatt tgcgtaattg gctaaatcat cgctatgcgt ggtttcacat
      781 tgacggttgt tctaacggca aaaacgcatt gtatagctac tggattggca aagcggcgga
      841 aggtttagat gaagatacat taagtggtca gattgccgag gatttcttgg cagataactt
      901 gctcgatgtg atcttggaat gtgtggcaga ttggggcgaa ttactgcgta ttttcgtgaa
      961 tccgccatac agcaacccac tgccgtttgt acaacgggcg gcagagctga aaaaagccgg
     1021 tcatatcgta gtaatgcttt tgccggcaga taaatctacc cgttggtatc aggttatcca
     1081 agaaaatgcc agtgaggtga tcgacattat cggtgggcgt attaatttta ttaatccgct
     1141 cacaggcgag gaagtgaaag gcaacaacaa aggctcgatg gtggcggtgt ttgatccgtt
     1201 tgtggaggga atagttaccc gtcaaatgcc gctggataag attaaggaac tgggcggtta
     1261 tgaaggataa cgaaggctgg gatggtttag atggctaaat atcaaatgat gaaattgccg
     1321 ggtggagtgt tatctccggc aagtgaagcg gacgaagaga gcttaaaagc acttaaaaat
     1381 cacgagcttt atgatgtaga aattaactta aaaatcaatc cggtgctaca ccgcaagatg
     1441 ttctcttttt tcaaattttg ttttgaacac tggtgtgcag aaaacggttt gcagaaattc
     1501 tcgaatgaaa agacacagtt tgatgagttt cgtaaggatt tactggttca agctggacat
     1561 tgtgaaatgg tgttcagttt gagtaatcca aatgaattta aactagtacc gaagtcaatt
     1621 tcttataaag aattagaaga tgacgtgaaa cgtcgtgagt tgtacgtggc aattactaat
     1681 gccgcaatgg caacagtgtt taaaggatgt aatgatgaaa acattatcaa tcagctttat
     1741 tcgtattttt aatttggtgt aattacagga aattaaaatg agtttttatt gagtggtttg
     1801 ataaaagaga caaaggaggt tggaatggct aataagccac ctaagcaaca caaatgcaaa
     1861 gagtgcggcg gttattacat caaattccaa agcacacagc aggtctgttc ggtaaagtgt
     1921 gcaatggcaa tgggtaaacg taagacagag actaaacgta aacaggcgga taaggcagag
     1981 cgtaaagagc gcaagcagag attagaaaaa ctgaaaagcc gttctgcttg gttgaaagac
     2041 ttacaaaata tttttaataa gtttatccgt ttaagagata aagatttacc ttgtatttcg
     2101 tgtggtcgcc atcatcaagg gcaatggcac gcagggcatt ataaaacggt cggtggcaat
     2161 ccggaattgc gatttaatga ggataacgta catcgccaat gctctgtgtg taataaccat
     2221 ctacacggca atattgtgaa ttatcgagta aatctgattg agaaaatcgg gcttgagcgt
     2281 gtggaatttt tagagcgtaa agaccaccca ccattaaaac ttactatcga acaaatcaaa
     2341 gatttaatca aagtctataa agcgaagtgc aaggagcttg agcgtgttac ctagaaaaat
     2401 taaaaacaag tggttagagc ctgaaaagca gaaatggatc gaagagttgc taaggttatg
     2461 gggggcgtgg gaatttggcg gattggattt agaaagtcgg gtaaatatga tttaccggct
     2521 gatgaaatct gcggaggggg ttaaaagtct ttcatctaga gaggtttgta acgatagcct
     2581 cggcacgctg atcaatgaga tttttacggt agtcaccaaa agagacgtaa tgttagctga
     2641 tatactcaaa caaaaatact ggttcgggcg ttcggagcgg caaatcgcta tttattatca
     2701 actaagggac agcgagggta gaaaagaaag acgttggaaa gaaattattc aagaaaaatg
     2761 caaaaaagcg gaaagaatta ttgcagatat tcttgaaagc aagatttcat ctagtcattc
     2821 tgcgaataaa ttaaaaaaat atcaatttaa cccttgattt tagtgcccat tagatatata
     2881 ttttgtgcta atggtggacg tagtataaat gatgtttacc aggttagaat tttacagccc
     2941 tgagcagaaa tgtttggggc ttttttatta ccccgagata gtggggtgga gtatgaagat
     3001 gccggataaa gatccgaatg tatggctggt aattagtgca tacattcagc aaaattataa
     3061 tgccattaca ggatttgtaa tggctttttt tatgtctatg ctcagggcgt ggtttttaca
     3121 acaaaaaagc acttatcgcc agcgatttct tgatggtgca atttgtggag cgttaacgct
     3181 ttcttgtatg tcgctattaa cccactttgg cgttggcgaa agtttatcta cctttgcagg
     3241 tggaatgatt gggtttgtgg gagctgagaa aatccgtgaa tttttatttg ccttaattcg
     3301 taaaaaaatc gaagtaaatg atgtcggctt taaccgaaaa agtgatgaat aagtaacggt
     3361 ggatatatgt taattagcga aagaaaattt aaacaagtgt tccccaatgc gattgacgga
     3421 atttatgccg gaattgccaa acacattgaa aaagccggtt gcaagacgaa agagcagcaa
     3481 gcaatgtttt tggctcaatg tggcgtagag tgtgccggtt ataaaacctt tgaagagaat
     3541 ttgaattatt ccgctacggc attattagcc acatttccga aatattttac ccaacacaat
     3601 gtcgtacttt atgctcgaga taagcgtgct attgctaacc gagtatatgc gaaccgtatg
     3661 ggaaacggca atgaacaaag cggagacggt tggaaatatc gtgggcgtgg tttaattcaa
     3721 attaccggta aggcaaatta tatcgagttt gctaaatggg agggattacc aagtttagtt
     3781 accagccctg accaaatcac gaataacctt gctctttgcg tgcaggttgc cgtgtggtat
     3841 tggcaaagcc gcaagttggc aaattacggt agcgatgtgt tgaaagtaac caagttgatt
     3901 aacggcggta ctcacgggct atcacaacgt acgaactact atcaaagatt aatggcggca
     3961 taaatgagca aatatattta tctcgttgcc gtcattttga ttttagggct gtgcggctgg
     4021 gttttgca
//
LOCUS       DANJWC010000180         4004 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.180, whole genome shotgun sequence.
ACCESSION   DANJWC010000180 DANJWC010000000
VERSION     DANJWC010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.180"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..698
                     /locus_tag="PWX02_002172"
     CDS             <1..698
                     /locus_tag="PWX02_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730142.1"
                     /translation="ALVLAEWTKEYHGKYDNFINDIKSDNWTSAAINFHLGMEKVPLN
                     DLIYKEIYNYWDKTSLKDESERIISASIEFSNYFANKYSENIEEGIKNAEKEFKKNYN
                     KSLRYDAKKAQQRVQKYVEKVNQKNPQALKDLDNISNLKKINENLKKAAKFTGIISSI
                     KDYYDLAEGFRNGIKSNDWEPFLKTSAQIGASALMSMLVASMITGPTTIGSIIISAAF
                     ISIGGYIISLASR"
     gene            1174..3213
                     /gene="malQ"
                     /locus_tag="PWX02_002173"
     CDS             1174..3213
                     /gene="malQ"
                     /locus_tag="PWX02_002173"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDL1730143.1"
                     /translation="MSNKQNTAHYFYSPNIKEYASPSAFEKIERLLNTDNDLQKNNQI
                     LPLVKVITQPNAEQQAVEISLNFTNVAKWQIVLENGEVRNGEIKAEQSVLTLPADLPL
                     GYHQLSISTENDEESCSLIVTPPKAFQPKPLEERKKLWGAFLQLYTLRSDNNWGIGDF
                     GDLKQFLANISDYGADFVGLNPIHALFPADPDAASPYSPSSRLWQNIIYIDVCAVEAF
                     QQSEQAQQWFNSEETQRQLAELRAKDYVDYSQVAQLKLKALQFAFEHFSQPSQQQSQT
                     EFNAFIEQAGEPLKIQATFDALHQWISSEFGEKGGWTNWAEEYQSYHSPKVAEFQTSQ
                     ATQIRFYMWLQFVANQQLAECNQFAQSLSMPIGFYRDLAVGVTQSGAETWADKALFVQ
                     EASIGAPPDMLAPNGQSWGLSPMHPHILQSRGYQPFIDLVRANMKHCGALRIDHILGF
                     ARMWWVANGESAKEGIYVRYPLEDLLSILALESQRHQCLVIAEALGIVPEGILDSLEQ
                     KGIFAYNVFYFEKAENAFKPLEDYPYQAMTTLSTHDLPTIQGYWKGYDFELGEKFNTY
                     PSKAVLRKLKEERSYDRLNIRNAVEKVMELEPNEVGVTMKFTHQLQRYVAHTHSALFG
                     TQPEDWLNMLEPVNIPGTSTEYPNWRRKLSATTEQIFANKDIQQLLKDINEIRKG"
     gene            complement(3281..>4004)
                     /locus_tag="PWX02_002174"
     CDS             complement(3281..>4004)
                     /locus_tag="PWX02_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HDL1730144.1"
                     /translation="SSFSTKLKQEFFSGWTTFEASWLIVFLAIQIGIFIYQPDTWIAT
                     IAAITGILCVVFVGKGKISNYLFGLISVSLYAYISYTFQLYGEMMLNLLVYVPVQFIG
                     FYFWRKNMTSENTVNNAGVEEVIAKALTAKQWVIVAITTIIGTFLYIELLKYLGSALA
                     ILDGATVVISIVAQILMVLRYREQWALWIIVNMMTIALWTAMYFQNGETSLPLLVMYV
                     MYLCNSIYGYYNWIKLHRKHQQ"
BASE COUNT         1388 a          762 c          743 g         1111 t
ORIGIN      
        1 gtgctttagt acttgccgaa tggactaaag aatatcatgg caaatatgat aactttatta
       61 atgacatcaa atcagataat tggacaagtg cagcaattaa ctttcattta ggtatggaga
      121 aagtaccatt aaatgatctt atttataaag aaatatataa ctactgggat aaaacatcat
      181 tgaaagatga atcagagagg attatatcag ccagtattga attcagtaac tattttgcta
      241 ataagtactc tgaaaacatt gaagaaggta ttaaaaatgc agaaaaagaa tttaaaaaga
      301 attataataa aagtctaaga tatgatgcta aaaaagcaca gcagagggtg caaaaatatg
      361 tcgaaaaagt caaccaaaag aatcctcagg cactaaaaga tctcgataac atatccaact
      421 taaaaaaaat taatgagaat ttaaaaaaag cagcaaaatt tactggtatt attagcagta
      481 ttaaggatta ttacgatcta gcagaagggt ttagaaatgg cataaagtct aatgattggg
      541 aacctttttt aaagacatct gctcagatag gtgcttcagc tttaatgagt atgttagtag
      601 cctcaatgat aacaggccca acgacaattg ggagtattat tattagtgct gcatttattt
      661 ctataggggg atatattatt tcactagctt cccgttagat agtctttgtt atactcaata
      721 gcctactata ctacctatag gctattgaac tatcatacat cagctctatt aaccaaaaaa
      781 acaaaaaaaa atattgcaaa taaaaataaa attattgaga taggtccaga gtacataatt
      841 aactcatact tttttttaag tttaatccta agagcctgag ctacaaaagg atataaaaat
      901 aaattaacca gcaaatataa tagagttata aaataaatga aaaatgtatt tgaattccag
      961 gcctcaaagc tacgcaaatc agaaaaaagc acagcacagg atattaataa aaacaaaaat
     1021 ggtatattta ataataaaac ataacccttt agagttaaat taaatttttt taaaatcata
     1081 tcacaaagac aaacctgttg cctactcttc ttactcatat taatcttcac aagatcgtta
     1141 ttttaacaaa tacagtaaag gaaaaaagtc ataatgtcaa acaaacaaaa tacagctcac
     1201 tatttttact ccccaaatat taaagagtat gccagcccct ctgcttttga gaaaatagag
     1261 cggcttttaa ataccgataa cgatttgcaa aaaaataatc aaattctacc gcttgttaaa
     1321 gtgattactc agccaaatgc cgagcaacaa gcggtcgaaa tttcgctgaa ttttacaaat
     1381 gtagcaaagt ggcaaattgt gcttgaaaat ggtgaggttc ggaacggtga aattaaggca
     1441 gagcaatcag ttctaaccct gcctgccgat ttgcctttag gctatcatca gctttctatt
     1501 tcaactgaaa atgatgaaga atcttgctcg ctgattgtaa caccgccgaa agctttccag
     1561 cctaagccat tggaagaaag gaaaaagctc tggggtgcat ttttacagct ttatactttg
     1621 cgttccgata acaactgggg aattggcgat tttggcgatc tcaaacaatt ccttgccaac
     1681 atttctgatt atggtgcaga ttttgtgggc ttaaacccta ttcacgccct ttttccggca
     1741 gatcctgatg cggcaagccc ttacagcccg tcctctcgac tttggcaaaa tattatttat
     1801 attgatgttt gtgcagtaga agcctttcaa caatctgaac aggctcaaca atggtttaac
     1861 agtgaagaaa ctcaacggca gcttgctgaa ttgcgagcga aagattatgt cgattacagc
     1921 caagtagcgc aactgaaatt aaaggctctg cagtttgctt ttgagcattt ttcacagcca
     1981 tctcaacaac aatcgcaaac cgagttcaat gcctttattg agcaagcggg cgagccatta
     2041 aaaatccaag cgacgtttga tgccttgcac caatggattt catctgaatt tggcgaaaaa
     2101 ggcggctgga caaactgggc ggaggaatac caaagttacc attcgcctaa agtagcagaa
     2161 tttcaaacct cacaggcaac gcagatacgt ttttatatgt ggttgcaatt tgtggcaaac
     2221 cagcaattag cagaatgcaa ccaatttgct caatcacttt caatgccgat tggtttttac
     2281 cgcgatttag ccgttggtgt tacccaaagc ggagcagaaa cgtgggcgga taaagcctta
     2341 tttgtgcaag aggcgtcaat cggtgcacca cctgatatgc tcgccccgaa cgggcaaagc
     2401 tgggggcttt ctccaatgca cccgcatatt ttacaaagcc gaggttatca gccgtttatt
     2461 gatttagtgc gtgctaatat gaaacattgt ggggcattgc gaatcgacca tattctcggc
     2521 tttgctcgta tgtggtgggt ggcgaacggt gaatcggcaa aagaagggat ttatgtccgt
     2581 tatccgctcg aagatttgct ctctattttg gcattagaaa gccaacgtca ccaatgttta
     2641 gtgattgcag aggctcttgg cattgtgcct gaaggtattt tggattctct cgagcaaaaa
     2701 ggcatttttg cctacaatgt tttttatttt gaaaaggcag aaaacgcgtt taaaccgctg
     2761 gaagattatc cttaccaagc aatgacaacc ctaagcaccc acgacttacc aaccattcaa
     2821 ggctattgga aaggctatga ttttgagcta ggcgaaaaat ttaacactta cccaagcaaa
     2881 gccgtattga gaaagctgaa agaagagcgt tcgtatgacc gcttgaatat ccgtaatgcg
     2941 gtggaaaaag tgatggaact cgagccaaat gaagtggggg tcacaatgaa atttacccat
     3001 cagctacaac gctatgtggc tcacactcac agtgcattat tcggtacaca gcccgaagat
     3061 tggctcaata tgttagagcc ggtgaacatt ccgggaacca gcaccgaata ccctaactgg
     3121 agaagaaaat tatccgcaac caccgagcag atttttgcaa ataaagatat tcaacaactg
     3181 ctaaaagata taaacgaaat tcgtaaagga taataacaag cggtcatttt taggtaaatt
     3241 tttacaaaaa atgaccgctt gtaatttgta tttattcgct ctattgctga tgttttctgt
     3301 gaagtttaat ccagttgtaa taaccgtaaa tagagttaca taaatacatc acatacatca
     3361 ccaacagcgg caagctggtt tcgccgtttt ggaagtacat tgccgtccat aatgcaatgg
     3421 tcatcatatt cacaattatc cataatgccc attgctcacg gtagcgaagt accatcaaaa
     3481 tttgtgccac aatcgaaata accactgttg caccatctaa tatagctaac gcactgccta
     3541 aatatttaag caattcaata taaagaaatg tgccgataat ggtggtaatg gcgacaatca
     3601 cccactgttt tgcggttaaa gctttagcaa ttacctcttc cacaccagca ttatttaccg
     3661 tgttttcgct ggtcatattt ttacgccaga aatagaaacc gataaactgc accggcacat
     3721 atacaagtaa attcagcatc atttcaccat ataactgaaa ggtataagag atataagcat
     3781 aaagcgaaac ggaaattaag ccgaatagat agttactgat ttttccttta ccgacaaata
     3841 ccacgcataa aatacctgtt attgccgcaa tagttgcaat ccaagtatcg ggttgataaa
     3901 taaaaatacc gatttgaatc gctaaaaaaa caatcaacca acttgcctca aaagtggtcc
     3961 aaccactaaa aaactcttgt tttaacttag ttgaaaaaga tgaa
//
LOCUS       DANJWC010000181         3865 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.181, whole genome shotgun sequence.
ACCESSION   DANJWC010000181 DANJWC010000000
VERSION     DANJWC010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.181"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(88..471)
                     /locus_tag="PWX02_002175"
     CDS             complement(88..471)
                     /locus_tag="PWX02_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730145.1"
                     /translation="MKTLKLGCIFSLALMLSSCTLTPEQQAERKAKQIRAEQDLQVQL
                     AKQCDVETAELMYQQFNPPLSQTEQEEKAFKKRYAEKVNAPMFQACYKLAWQNYKAQV
                     ELEEIRWNYDRDYMYRGWRYCYYCW"
     gene            complement(501..1070)
                     /locus_tag="PWX02_002176"
     CDS             complement(501..1070)
                     /locus_tag="PWX02_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDL1730146.1"
                     /translation="MNETTINDFSLLCRLFGNLFYRHPTDPILAGTFNWLAQGGLRQV
                     WALNTDSQSENALTLLQKNANPTQLAASYQALFAENGAISPTISAYGLSVNNFVAFRQ
                     ERAMPELENADHIGLLFLTASWIEDNLDSALAQKILFEQFLLPCAGKFLGKIEAFDNG
                     FYKALAQLSRDALSAMADELEESELEVVE"
     gene            1129..1974
                     /gene="nfo"
                     /locus_tag="PWX02_002177"
     CDS             1129..1974
                     /gene="nfo"
                     /locus_tag="PWX02_002177"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDL1730147.1"
                     /translation="MKYIGAHVSASGGVENAVLRSVEIGANAFALFTKNQRQWQAPAL
                     KAETIEKFKRFCKVHHFSAEQILPHDSYLINLGNPEAEALEKSRVAFIDEMSRANQLG
                     LKLLNFHPGAHLKQISEQDCLARIAESINIAVEKVPNVIAVIENTAGQGSNLGWHFDH
                     LATIIEQVEDKSRVGVCLDTCHLFSAGYDISSYEKSEAVFNDFNQTVGFHYLRGMHLN
                     GSKTPLGSRVDRHHTLREGTIGTEVFAFIMQNMHFNHIPMILETISPEIWADEIKFLR
                     TLELK"
     gene            2102..2674
                     /locus_tag="PWX02_002178"
     CDS             2102..2674
                     /locus_tag="PWX02_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL1730148.1"
                     /translation="MKKNLFATTFKPVFIASILSVFGGYSSIAVASLSSKPATPNQII
                     ASKAERLAKSSSVFTNRANLPTSSAHTANYSGRINSVYHNWVGTRYRLGGITKAGIDC
                     SAFVREVMGKAFSKDMPRSTAEQRHVGHSISKSDLRPGDLVFFRKNNHVGVYIGNGKF
                     VHASSSRGVTTSSLSESYWSRNYTQSRRVI"
     gene            complement(2741..3199)
                     /locus_tag="PWX02_002179"
     CDS             complement(2741..3199)
                     /locus_tag="PWX02_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL1730149.1"
                     /translation="MASIRQISDQTAAYQPIPLYRDFNSSRSVKLDLNSLCIKRPRDT
                     FFIHVKNPNLIAWGIELDDLLIVEQTNQYLVNDLLVLEKDGEYKFYQFFNEIQNGEGI
                     AEKILFSLDVSESNLRITDWAEVKIAGVITNVVHQMRTRGAFTQAKQRVA"
     gene            complement(3373..3756)
                     /gene="grcA"
                     /locus_tag="PWX02_002180"
     CDS             complement(3373..3756)
                     /gene="grcA"
                     /locus_tag="PWX02_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HDL1730150.1"
                     /translation="MIKGVQITESSNSNLLNSFWLLDEAKNEARCLAAKAGFEEDQVV
                     AISELGDIAYREVPVNVAPTIKVEGGQHLNVNVLRRETLEDAIQNPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVITRTFTESL"
BASE COUNT         1178 a          680 c          749 g         1258 t
ORIGIN      
        1 aaaataaacc tatatcaatt aataagcggt catttctcgc caattttttg caaggtgtta
       61 aaaaaatgac cgctcataca atgagattta ccagcaataa tagcagtaac gccagcctct
      121 atacatatag tctctatcgt aattccaacg aatttcttca agttctactt gtgccttgta
      181 attttgccaa gcaagcttat aacaggcttg gaacattggg gcattgactt tttcggcata
      241 gcgtttctta aatgctttct cttcttgttc ggtttggcta agtggcggat taaattgttg
      301 gtacattaac tccgccgttt ctacatcaca ttgtttggcg agctgaactt gcaaatcttg
      361 ttctgcccga atttgcttcg ctttacgttc agcttgttgc tctggagtta aagtacagct
      421 acttaacata agtgcgagtg aaaaaatgca gcctaatttg agtgttttca ttgctgaatc
      481 ctctagttga aattggtgat ttattctacc acttctaact cactttcttc aagttcatca
      541 gccatagcac taagggcatc acggctaagt tgtgcgaggg ctttataaaa accattgtcg
      601 aaagcttcta tttttcctaa gaatttgcct gcacaaggta gtaaaaattg ttcgaataat
      661 attttttgag ctaaggcaga atcgagatta tcttcgatcc aagatgccgt gagaaatagt
      721 aacccaatgt gatcggcatt ttctaattca ggcattgccc gctcttgtct aaaagctaca
      781 aaattattaa ctgataatcc ataggctgaa attgttggag aaatagcccc attttcagca
      841 aaaagcgctt gatagcttgc tgctaattgc gtagggttag cattcttttg taagagagtc
      901 agcgcatttt cactttggct atccgtattt aatgcccaga cttggcgtaa tcctccttgg
      961 gcaagccagt taaatgtgcc agctaaaata gggtcggtag ggtgacgata aaataagttg
     1021 ccaaacaaac ggcaaagcag ggaaaaatcg ttaatggtag tttcgttcat aatttgaaat
     1081 agatagaaaa aagaattgtt agtatagcag aacacaagag aggactgaat gaaatatata
     1141 ggcgcacacg ttagtgcttc aggtggggtg gaaaatgcag ttttgcgttc cgtagaaatt
     1201 ggtgctaatg catttgcgtt atttactaag aatcaacgtc aatggcaggc accggcatta
     1261 aaagccgaaa ctatcgaaaa atttaagcgt ttttgtaaag tgcatcattt ttcagcagag
     1321 caaatcttac cacacgatag ctatttgatt aatttaggaa atccggaagc ggaggcattg
     1381 gagaaatctc gtgtagcatt tattgatgag atgtctcgag caaatcaatt aggtttaaaa
     1441 ttgttgaatt ttcacccggg ggcgcattta aagcaaattt ccgagcaaga ttgtttggct
     1501 agaattgcag aatcgatcaa tattgcagtt gaaaaagtac cgaatgttat tgccgtgatt
     1561 gaaaatacgg caggacaggg ttctaattta ggttggcatt tcgatcattt ggcaactatt
     1621 attgagcaag ttgaggataa aagccgagtg ggggtgtgtt tagatacttg tcacttattt
     1681 tctgccggct atgatattag ttcttatgaa aagagcgagg cagtatttaa tgattttaac
     1741 caaacggttg gttttcatta tttaagggga atgcacttaa atggttcaaa aacaccatta
     1801 ggcagcagag tggatcgtca tcatacctta agagaaggca caataggaac cgaagtgttt
     1861 gcatttatta tgcaaaatat gcattttaac catattccta tgattttaga gacaatttcg
     1921 cctgaaattt gggcagatga aataaaattt ctacgcacat tggaactaaa ataggttata
     1981 ctggtctgag tagtgggtct agcagagtct agaccttatt ttgttatctt ttttaatctt
     2041 ttagaagatt tagttaagga atttaccgct gtaattgttc tggcgagacg agacataaat
     2101 tatgaaaaaa aatctttttg caacaacctt caagcccgta tttattgctt ctattttgtc
     2161 tgtttttgga gggtattctt ccattgctgt agcaagttta agttctaaac cggccacacc
     2221 aaatcaaatc attgcaagta aagcagaaag attagctaag tcttcttcag tatttaccaa
     2281 tcgagcaaat cttccaactt cttccgctca tacagcgaat tatagtggtc gtattaatag
     2341 tgtttatcat aattgggtag ggacacgcta tcgtttaggc ggcataacaa aagcaggaat
     2401 tgattgttcc gcctttgtgc gtgaagtaat gggcaaagca tttagtaaag atatgcctcg
     2461 ttcaactgca gaacaacgcc atgtcgggca ttccatttca aaatctgatt tacgcccagg
     2521 tgatttagtc tttttccgta agaataacca tgtaggggtt tatattggta atggtaaatt
     2581 tgtacacgct agttcaagta gaggggtaac caccagctca ttgtcagaaa gctactggtc
     2641 aagaaactac acacaatctc gccgtgtaat ttaataaatt acaattccaa gatgaaaaga
     2701 acactcactt ttgcgagtgt tcttttttaa ttaaaaaagt ttatgctacg cgttgcttag
     2761 cttgtgtaaa tgctccacga gtacgcattt gatgaaccac attagtaatt acacctgcta
     2821 tttttacttc agcccaatca gtaattctaa gatttgattc gctcacatct aatgaaaata
     2881 aaattttttc agcaatacct tccccattct gaatttcatt aaagaattga tagaatttat
     2941 attccccatc tttttcaagc accaatagat cattaactaa atattggttg gtctgttcta
     3001 caatcaataa atcgtctagt tcaattcccc acgcgattaa atttgggttt ttcacgtgga
     3061 taaaaaaagt gtctcttggg cgtttgatac aaagtgagtt aagatcgagc tttacagagc
     3121 ggctgctatt gaaatcccga taaagcggaa tcggctgata agctgccgtt tgatctgaaa
     3181 tttgtctaat tgaagccata gtaaaatcct ctatctgttt ttttatacag ggtattttac
     3241 tggctaaaat accagtgtca accattattt tggaattttt tgtaaattta accagtgttt
     3301 tgaccgcttg ttatccttta aaaagcaata aaaaagcacc catagggtgc ttcttatgct
     3361 taaaagctgt tattataggc tttcagtgaa tgtacgagta ataacatcgc gttgttgctc
     3421 aggggttaat gagttgaaac gaactgcata acctgaaaca cggatcgtta attgaggata
     3481 tttctcaggg ttttgaatcg catcttctaa tgtttcacgg cgtaaaacgt ttacgtttaa
     3541 gtgctgacca ccttcaactt taatcgttgg tgcaacatta acgggtactt cacggtatgc
     3601 gatatcacct aattcgctga tagcaacaac ttgatcttct tcaaatcctg cttttgcagc
     3661 taaacaacga gcttcatttt tagcttcgtc taataaccag aaagagttta ataagttgct
     3721 gtttgatgat tctgtgattt gtacaccttt aatcattttg atatctcctc taagattgaa
     3781 attataaaaa aattttaaaa attcaaattt atttgaagtt attgtacccc tttttagata
     3841 aaaagcgata aaaatttaac cattt
//
LOCUS       DANJWC010000182         3846 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.182, whole genome shotgun sequence.
ACCESSION   DANJWC010000182 DANJWC010000000
VERSION     DANJWC010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.182"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..1606
                     /locus_tag="PWX02_002181"
     CDS             <1..1606
                     /locus_tag="PWX02_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HDL1730151.1"
                     /translation="VQEKIAIFAQNMPRWTITDIAAMQIRAVAVPIYATSTTKQLEYI
                     LNDGDIKILFVGDQEQYDQAIEIANNCSLLIKIVAMKDSIDLRDFPIAQHWQDFIKVN
                     QDEAELTKRLNDKCLEDLFTLIYTSGTTGEPKGVMLDYANLAHQLQAHNKALPHIDEN
                     DISLSFLPLSHIFERAWVTYVLYRGAVNCYLEDPQQVRQALSTVRPTLMCAVPRFYEK
                     IYTAVWDKVQQAPIHRRALFKWAIRTAEDNLFGKKSAFFHRLQYIMADKLVLGKLRAL
                     LGGRIRMMPCGGAKLDPSIGLFFQSIGINVKLGYGMTETTATISCWQEKGFNINSTGT
                     LMPDVEVKISEENEILVRGGLVMKGYYKKPEATAQAFTAEGFLKTGDAGELDSEGNLY
                     ITDRIKELMKTSNGKYIAPQYIEGKIGKDKFIEQIAVIADAKKYVSALIVPCFDSLEE
                     YAKKLNIRYQDRLELIKHYEVVRMFEQRINELQKDLASFEQIKKFTLLSQAFSTKMEE
                     ITPTLKLRRKVILERYKQQIEKMYES"
     gene            complement(1639..1983)
                     /locus_tag="PWX02_002182"
     CDS             complement(1639..1983)
                     /locus_tag="PWX02_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDL1730152.1"
                     /translation="MIQRFEVGSRFSEMAIHNGVAYLAGQVPLDDTADAYTQTQQVLA
                     EIDKWLAKANSDKSKILMATVYLKEMADYAEMNRAWDEWVAPNNAPPRAAVEAKLANP
                     NWKVEIVVTAAV"
     gene            complement(1992..2540)
                     /locus_tag="PWX02_002183"
     CDS             complement(1992..2540)
                     /locus_tag="PWX02_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730153.1"
                     /translation="MAKLFANSTACLYLENMKKLIFLGLSALALAGCPSTISNPNQLI
                     GQWQCTIEYDDFNIRTVDNLRFSTNGTMTNQGGIYYPIQKSIFEYSLQQNGRWSVKNG
                     AIVYRIVSESVQRSHRSQIWSELQRDAELQQFEENLFSSLSDSDNNKTVELIITDFDE
                     KEMDIKQEIKGHKAYKGQCIKQ"
     gene            complement(2567..3604)
                     /locus_tag="PWX02_002184"
     CDS             complement(2567..3604)
                     /locus_tag="PWX02_002184"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDL1730154.1"
                     /translation="MALKELDWANLGFSYIKTDYRFIAHWKDGKWNEGELTQDNTLHI
                     HEGSTALHYGQQCFEGLKAYRCKDGSINLFRPDQNAERMQRTADRLLMPRVPTELFIR
                     ACKKVVKANQDWLGPYGSGATLYLRPFLIGVGENIGVKAAPEFIFSVFCCPVGAYFKG
                     GLAPSNFITTDYDRAAPMGTGGVKVGGNYAASLLPHELAVEQSSTTRKFADAIYLDPK
                     THTKIEEVGAANFFGITKDNKFITPISESILPSITKYSLLHLAQERLGMEAIEGDVYI
                     DQLDQFAEAGACGTAAVITPVGGIQHKDKFHVFYSETEVGPVTRRLYAELTGIQFGDV
                     EAPQGWIVKVE"
     gene            complement(3746..>3846)
                     /locus_tag="PWX02_002185"
     CDS             complement(3746..>3846)
                     /locus_tag="PWX02_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HDL1730155.1"
                     /translation="GELVGGCDIVLEMLQKGELQTLLKETAAKHAA"
BASE COUNT         1161 a          800 c          735 g         1150 t
ORIGIN      
        1 cgttcaagag aaaatcgcta tttttgcaca aaatatgcct cgttggacaa ttaccgatat
       61 tgctgcaatg caaattcgtg cggtagcagt gccgatttat gcaaccagta ccaccaaaca
      121 acttgaatat atcctgaatg acggggatat aaaaatcctc tttgttggcg atcaggaaca
      181 atatgatcaa gccatagaaa tagcgaacaa ctgttcgcta ttaataaaaa ttgtcgcgat
      241 gaaagactca atagatctgc gtgatttccc tatcgctcaa cattggcaag atttcattaa
      301 ggttaatcaa gatgaagctg aattaaccaa acgtttaaat gataaatgct tagaggattt
      361 attcacttta atttatacct cgggaacaac cggtgagcct aaaggtgtta tgttagatta
      421 tgctaattta gctcatcaac ttcaggcaca taataaagca ctccctcata ttgacgaaaa
      481 tgatatatcc ctttcatttt tacctttatc gcatatcttt gaacgtgctt gggtcactta
      541 tgtgctatac agaggggcag taaattgcta tctggaagat cctcaacagg tacgtcaagc
      601 actctcaacg gtacgaccta cattaatgtg tgccgtacca aggttctatg aaaaaatcta
      661 tactgcggtg tgggataaag tccaacaagc accgatacat cgccgagcgt tatttaaatg
      721 ggcaatccgt accgccgaag acaatctgtt tggtaaaaaa tcagcctttt tccaccgctt
      781 gcagtacatt atggcagata aattggtgct aggaaaatta cgagccttac taggcggtcg
      841 tattcgtatg atgccgtgtg gaggagctaa attagatccg tctatcgggc tatttttcca
      901 aagtataggc atcaatgtaa aacttggata cggaatgacc gaaactactg ccactatttc
      961 atgctggcag gaaaaaggct ttaatatcaa ctcaaccggt accttaatgc ctgatgttga
     1021 ggtcaaaatt agtgaagaaa atgaaatttt agtgcgtgga ggcttggtta tgaaaggtta
     1081 ctacaaaaaa ccggaagcca ctgctcaagc ctttactgct gaaggttttc taaaaacggg
     1141 agatgccggt gaactggatt cggaaggcaa tctttatatt accgatcgta ttaaagaatt
     1201 aatgaaaact tcaaacggta aatatattgc cccacaatac attgaaggaa aaataggtaa
     1261 agataagttt attgagcaaa ttgcggtgat tgctgatgcc aaaaaatatg tttctgcgtt
     1321 gatagttcct tgctttgata gcctcgaaga gtatgcaaaa aagctcaata ttcgctatca
     1381 agacagatta gaactcatta aacattatga ggttgtcaga atgtttgaac aacgcattaa
     1441 cgaattacaa aaagaccttg caagttttga acagatcaaa aagtttacac tactttcgca
     1501 agcatttagc accaaaatgg aagaaattac gcctacatta aaattaagac gaaaggtcat
     1561 tctagaacgc tataagcaac aaatcgaaaa aatgtacgaa tcataatgaa aaacagagat
     1621 cgtctttctg cttttcattt ataccgcagc ggtaaccaca atttcgactt tccaatttgg
     1681 attggctaat ttagcctcca ctgccgctct tggtggagcg ttatttgggg caacccattc
     1741 gtcccacgca cggttcattt ccgcataatc tgccatctct tttaaataaa ccgtagccat
     1801 tagaatttta cttttatcgc tgttggcttt tgccagccat ttatcaattt ctgcaagcac
     1861 ttgctgggtt tgggtgtaag catcggcggt gtcatctaac ggcacttgcc ctgccaagta
     1921 agccacaccg ttatgtattg ccatttccga aaaacggcta cctacttcaa aacgttgaat
     1981 catttttacc tctactgttt tatacattgc cctttataag ctttatgccc ttttatttct
     2041 tgcttgatat ccatttcttt ttcatcaaaa tcagtgatga ttagttccac tgttttatta
     2101 ttatcactat cagaaagtga gctaaataaa ttctcttcaa attgttgtag ctccgcatca
     2161 cgttgtaatt cagaccatat ttgtgaacga tggctacgct gtacagattc tgagacgata
     2221 cgataaacaa tagccccgtt tttcacactc cagcgcccat tctgttgtaa tgaatactca
     2281 aaaatcgact tttgaatagg gtaatatata ccaccttgat tggtcatagt cccatttgtg
     2341 gaaaatcgta aattatccac cgtacgaata ttaaaatcat cgtactcaat agtgcattgc
     2401 cattgcccaa tcagttgatt aggattagaa attgttgagg gacaaccagc taatgcaagt
     2461 gctgataaac ctaaaaaaat taactttttc atattctcta aataaagaca agcggttgaa
     2521 tttgcaaaaa gttttgccaa ttcgaccgct tgttacttgt gtaaaattac tctactttta
     2581 caatccagcc ttgcggtgct tcaacatcgc caaattgaat accggttaat tctgcgtata
     2641 aacggcgggt gacaggcccc acttcggttt ctgaatagaa gacgtggaat ttgtctttat
     2701 gttgaatacc accaaccggc gtaatcaccg cagccgtacc acaagcaccg gcttcggcaa
     2761 attggtctaa ctggtcgata tacacatctc cctcaatcgc ttccatacct aaacgctctt
     2821 gagcaaggtg tagcaatgag tatttggtaa tacttggcaa aatcgactct gaaatcgggg
     2881 tgatgaattt gttgtctttg gtaatcccaa agaagttcgc cgcccccact tcttcaattt
     2941 tggtatgggt tttcggatct aaataaatcg catcggcaaa tttacgggta gttgaacttt
     3001 gctcaaccgc caattcgtgc ggtaataaac tcgcagcgta gttaccgcct actttcacac
     3061 cacctgttcc cattggggct gcacggtcat agtcggttgt aatgaagttt gagggtgcta
     3121 agccaccttt gaaatatgcc cccaccggac agcagaatac ggaaaaaata aattcaggtg
     3181 cagctttcac cccaatattt tcgcccacgc cgattaaaaa cggacgtaaa taaagggttg
     3241 cccccgaacc gtaaggccct aaccaatctt gattggcttt taccactttt ttacaggcac
     3301 gaataaacag ctcggttggc acacgtggca ttaataaacg atctgcggtg cgttgcatac
     3361 gctcggcatt ttgatccggg cggaataagt taattgagcc atctttacag cgataggctt
     3421 ttaacccctc aaaacattgt tgcccatagt gcagggcagt tgagccttcg tgaatatgca
     3481 gtgtattgtc ttgagttaat tcgccttcat tccattttcc gtctttccaa tgtgcaatga
     3541 aacggtaatc ggttttgata taagaaaagc ctaaatttgc ccaatctaac tcttttaatg
     3601 ccatgttgtg tccttttatt tttatgttac gaaacaattt tgacattcta aaacaaaata
     3661 gctcttgagg taagaagttt aagcctaaat cgcaagaaaa aattaaggct cttaaacata
     3721 agagccttaa catactggtt tgaaattatg ctgcgtgttt tgctgcggtt tcttttaata
     3781 aggtttgaag ttcgcctttt tggagcattt ctaatacgat gtcacaaccg ccaactaatt
     3841 cacctt
//
LOCUS       DANJWC010000183         3828 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.183, whole genome shotgun sequence.
ACCESSION   DANJWC010000183 DANJWC010000000
VERSION     DANJWC010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.183"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..417
                     /gene="def"
                     /locus_tag="PWX02_002186"
     CDS             <1..417
                     /gene="def"
                     /locus_tag="PWX02_002186"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005621146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL1730156.1"
                     /translation="DDMFETMYEHEGIGLAAPQVGVLKRVITIDIEGDKTNQVVLINP
                     EILESSGETGIEEGCLSIPGHRALVPRKEKVKVKALNRQGEEIIIDADDLFAICIQHE
                     IDHLNGVLFVDHISNLKRQRIKEKMQKLKKQIARAK"
     gene            511..1437
                     /gene="rfaD"
                     /locus_tag="PWX02_002187"
     CDS             511..1437
                     /gene="rfaD"
                     /locus_tag="PWX02_002187"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HDL1730157.1"
                     /translation="MIIVTGGAGMIGSNIVKALNEIGRKDILVVDNLKNGEKFINLVD
                     LDIADYCDKEDFIASIIAGDDFGEIDAVFHQGACSATTEWDGKYLMQNNYEYSKELLH
                     FCLDRQIPFFYASSAATYGGRSDNFIEKREFEGPLNAYGYSKFLFDEYVRRILPEAES
                     PVCGFKYFNVYGPREQHKGSMASVAFHLNNQMLKGENPKLFAGSEQFLRDFVYVEDVA
                     KVNLWAWQNSISGIFNLGTGKAESFQEVANAVIKYHGKGEIETIPFPDHLKSRYQTFT
                     QADLTNLRAAGYTGTFKTVAEGTAEYMAWLNR"
     gene            complement(1513..2043)
                     /gene="fabA"
                     /locus_tag="PWX02_002188"
     CDS             complement(1513..2043)
                     /gene="fabA"
                     /locus_tag="PWX02_002188"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017807287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HDL1730158.1"
                     /translation="MNSCTPIIKPSYTYEELLLSGKGELFGEKGPQLPAPPMLMMDRI
                     IEMNQEKGNFEKGYIEAEFDIKPDLWFFNCHFINDPVMPGCLGLDAMWQLVGFYLGWI
                     GGEGKGRALGVGEVKFTGQILPTAKKVTYRIHMKRVVNRKLVMGVADGEVEVDGRVIY
                     TATDLKVGLFQDTTAF"
     gene            complement(2156..3793)
                     /locus_tag="PWX02_002189"
     CDS             complement(2156..3793)
                     /locus_tag="PWX02_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL1730159.1"
                     /translation="MTLSLLSSQKLVMQNDFSRLDEKLNFLDFQPQAKQALNQFLQSQ
                     FGALLVLKSEIFPEFINEITEYLKKYSVDLPIISKMDFNKNSLFGYSVFLERELKTEY
                     VKGAIHEADKGILIVNIRSLLLDITQWDKLKQALLFGEYEPNSVNHSPYLSAKEKSTF
                     KLILVGEREDISTLSAYDDGLYQFGQYTEIKSYLSLSDNVEQWGGYVQNYAENLINKR
                     LTSTALNQFLQAYIRESESRELVSISPTLLRKHLLGIQNFYPNVTEIDEVKGYFDYLE
                     QQSSILNQYTVGDILTNQLYIETEGEEIGQINGLSVIEFEGIPHSFGEPLRMSCNVQY
                     GDGEITDIERKVELGGNIHSKGIIIAQSCLANLLELPTQLPFSASIAFEQSYSEIDGD
                     SSSLAIFNVLVSALAKVGLPQSIAVTGAIDQFGNVLSVGGVNQKIEGFFNICHARQLT
                     GKQGVIIPAACVSHLSLSSEVVEAVEQGLFKIWTVENVFDAIAILFERDFYDDEKAPN
                     SAQTALFTLIHQNLDERESEETSGSFLSKICKKIKLD"
BASE COUNT         1211 a          795 c          695 g         1127 t
ORIGIN      
        1 gatgatatgt ttgagacaat gtacgaacac gaaggtattg gcttagctgc accgcaagtg
       61 ggcgtactta agcgtgttat tactattgat attgagggcg acaaaaccaa tcaagtggtg
      121 ttaattaacc ctgaaatttt agaaagcagc ggtgaaaccg gcattgaaga gggctgtctc
      181 tccattcccg ggcatcgtgc cttagtgcca cgcaaagaga aagtgaaagt caaagccctt
      241 aaccgccaag gcgaagaaat tatcatcgat gccgatgatt tatttgcgat ttgtattcaa
      301 cacgaaatcg accatttaaa cggcgtgctt tttgttgacc acatatccaa tttaaaacgc
      361 caacgcatca aagaaaaaat gcaaaaattg aaaaagcaaa ttgcacgagc gaaataacca
      421 ggcacaagcg gttaaaatgg cttaaaaatt tgcaaattaa gtgtgatgac acgataataa
      481 ccacagttca actttagaaa ggataaaaat atgattatcg tcaccggcgg cgcaggtatg
      541 attggcagta acattgttaa agccttaaat gaaatcggtc gcaaagatat tttagtagta
      601 gataacctca aaaacgggga aaaatttatc aacttagtgg acttagatat tgcggattat
      661 tgcgataaag aggatttcat cgcctctatt atcgccggcg atgattttgg tgagattgat
      721 gcggttttcc atcaaggggc ttgctcggca accaccgaat gggacggcaa atacttaatg
      781 caaaataact acgaatattc aaaagagtta ttgcattttt gcttagatcg ccaaattcct
      841 ttcttctatg cctcaagtgc ggcaacctat ggcggacgtt cggataattt tatcgagaaa
      901 cgggagtttg aaggtccatt aaatgcttat ggctactcca aattcctgtt tgatgaatat
      961 gtccgccgta ttttgcctga ggcagaatca ccggtctgtg gtttcaaata tttcaacgta
     1021 tacgggcctc gtgagcaaca caaaggttca atggcaagcg tggcattcca cttaaacaac
     1081 cagatgttaa aaggggaaaa tccaaaacta tttgccggct cagagcagtt cttgcgtgat
     1141 tttgtttatg tggaagatgt ggcaaaagtg aatctttggg cgtggcaaaa cagcatttct
     1201 ggcattttca acctcggcac cggaaaagcg gaatctttcc aagaagttgc gaatgcggtt
     1261 atcaaatacc acggcaaagg cgaaattgaa accatcccat tcccggatca tttaaaatcc
     1321 cgctaccaaa cgttcactca agcggactta accaacctaa gagccgctgg ctacacaggc
     1381 acatttaaaa ccgttgctga aggaacagcg gaatatatgg cgtggttgaa tcgctaatca
     1441 aacgttgtta aaaaagcaga aagagctacg aaatgtagct ctttttgtta taaggttata
     1501 aggttataag gattagaatg ctgtcgtatc ttggaataag cctactttta aatcagtagc
     1561 agtatagatc actcgcccgt ccacttcgac ttcaccatca gcaaccccca tcactaactt
     1621 acgattgacc acacgtttca tatggatacg gtaagtaact tttttcgctg tcggtaaaat
     1681 ttgtccggtg aattttactt cacctacgcc caatgcacgc cctttgcctt cgccaccaat
     1741 ccagccgaga taaaaaccaa ccagttgcca catcgcatct aagcctaagc aacccggcat
     1801 tacaggatcg ttaataaaat ggcagttaaa aaaccataaa tccggtttaa tatcaaactc
     1861 agcttcaata tacccttttt caaaattgcc tttttcttgg ttcatttcaa tgatgcgatc
     1921 catcattaac atcggtggag ccggcaattg aggccctttt tcaccgaata attctccttt
     1981 tcctgatagc aaaagctctt cgtaagtata gcttggtttg ataattggtg tacaactgtt
     2041 cattattttt aaaatcctgt aaagtgaaaa aagcctgtta tatcaataac tgataaatca
     2101 ggctgatagt aacagacttt taattcagtg aacagttgta agctgacttg tcggatcagt
     2161 ctagctttat ctttttgcaa attttgctta aaaatgaacc gcttgtttct tcactttccc
     2221 gctcatctag attctggtga atcaaggtaa ataatgctgt ttgggctgag ttaggggctt
     2281 tctcatcatc ataaaaatcc cgctcaaaca gaatcgctat ggcatcaaac acattttcca
     2341 ccgtccaaat tttaaataga ccttgctcca ctgcctcaac aacttctgaa cttaaactca
     2401 aatggctaac acaggccgcc ggaataatca caccttgttt ccccgttaat tggcgagcgt
     2461 gacaaatatt aaagaagcct tcaattttct gatttacccc gcccacgctt agcacattcc
     2521 caaattgatc gatagcacct gtcaccgcaa tagattgtgg caagccaact ttagctaagg
     2581 cactcactag tacattgaaa atcgcaagag aagaactatc cccatcaatt tcactatagg
     2641 attgttcaaa agcaatcgaa gctgaaaacg gtaactgggt tggtagttct aacagattcg
     2701 ctaaacaaga ttgagcgata ataatacctt tggagtggat atttccccct agctccactt
     2761 tacgctcaat atctgtaatt tcgccatcac catattgtac attacaactc atgcgtaagg
     2821 gctcgccaaa actatgcgga atgccttcaa actcaatgac cgataagcca ttgatctgcc
     2881 caatttcctc tccttcggtt tcaatataaa gttgattagt cagaatatca cccaccgtat
     2941 attgatttaa tatcgatgat tgctgttcta aataatcaaa ataacctttt acctcgtcaa
     3001 tttcggtcac atttgggtaa aaattttgaa ttcctaataa atgttttctc aataatgtag
     3061 gggaaataga caccaactct cggctttcac tttcacgtat ataggcctgt aaaaattgat
     3121 taagcgctgt ggaagttaat cgcttattga tgagattctc agcataattc tgaacataac
     3181 ctccccactg ctccacatta tccgataatg ataagtagga ttttatttcg gtatattgcc
     3241 caaattggta caacccatca tcataagcag ataaagtaga aatatcttct ctttccccta
     3301 ctaaaatcaa tttaaatgtt gatttctctt tagccgataa atagggtgag tgattaacag
     3361 aatttggctc atattcacca aataataatg cctgttttag cttatcccat tgggtaatat
     3421 ccaataagag cgaacggata ttaacaatta aaattccctt atctgcctcg tgaattgcac
     3481 ctttaacata ttctgttttc agctctcttt ccaaaaacac agaataacca aatagactat
     3541 ttttattgaa atccattttg ctaataatcg gcagatctac actatatttt ttcagatatt
     3601 ccgtgatttc attgataaat tccggaaaaa tctcagattt taagactaat aaagcaccaa
     3661 actgagattg taaaaattga tttaacgctt gttttgcttg aggttgaaaa tctaaaaaat
     3721 tcaatttctc atctaaacga gagaaatcat tttgcattac taatttttgg ctactaagta
     3781 aagaaagggt cacaaatctg cctcattatt gaaaatttgg tttattat
//
LOCUS       DANJWC010000184         3828 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.184, whole genome shotgun sequence.
ACCESSION   DANJWC010000184 DANJWC010000000
VERSION     DANJWC010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.184"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..3528)
                     /locus_tag="PWX02_002190"
     CDS             complement(<1..3528)
                     /locus_tag="PWX02_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730160.1"
                     /translation="MVTTVKDNEIQFSLNSTLSVGKDGKDGQPGEAGTIGVKGQDGKD
                     GVSIKGDDSKDGVPGEASVVVGRNGADGIDGKVGVNGKDGASVVLNGKDGSIGLTGPK
                     GADGQPGASTNIAVKDGKQGVDGTGKDGLPGDNGETRIVYKNGDKEEQVANLNDGLIF
                     TGNNADTLNRHKLNTVVNVVGEGVDKAASAVFKSAAGNINVKADGTDKLEIQLNRELK
                     DLTNAKFVEYKQGDDGKEVVDNTKPSTELTNKGITITPANSENGADAKPVSLTDKGLD
                     NGNNQIVNVKSGIDGVNGQDGQDGQPVKSVNDLTEEQLKEIGTNAANINDVRNATDDL
                     VKTGFKITADNSKLADDAKEDTVELGQTVEYRSESGNIVTTVHDNKIDFDLANNLNVG
                     GKDGKDGLTFKSADGAQGVNGEDGENGLPGQNGTTRIVYQPTDKDGNPQGDPETVATL
                     NDGLIFTGNNELLNRHKLNTVVKIVGEGVDKATSEAFKSAAGNINVVANEKDQLEIQL
                     NRDLKDLATANFVEYKEDGSINADAPKTVVNNNGLTVTPKDGKDGKDGNVVSLTKDGL
                     NNGGNQIVNVKSGIDGINGQPGKDGKAVESVNDLTDEQLEKVGTNAANIKDVRNATDS
                     LINKGFNITADNSELEDGAKEDNVKLGETVNYTSKDKNIITTVSNNAIDFALNNNLTI
                     GGPGKDGKDGVDGKVGVKGADGTAGVTLNGKDGSIGLTGPKGQDGKDGASATISVKDG
                     AKGLDGNDGKDGESKTRIVYEKPDGTTEEVATLNDGLRFVGDDGKVINKKLNETLQIS
                     GGDTDLEDLSDDNIGVVNKDGKLMVKLARNIDLTDEGSVTIGDTYIDTNVVGVGDSAL
                     TGDSLTVGGDNSITVDGNKGTIGGLTNKTFDPNNFTSGQAATEDQLKQVYDVANSGWD
                     IAANGNNSTNVGPKGKVSFNNEDGNIIITKETTDNNVTFALNNDLTIGGPGKDGTIGI
                     NGKDGSNGTMTFEQGKPGVDGKDGETKTRIVYETKDKDGNPVKEEVATLNDGLKFVGD
                     DGKVITKKLNETLSITGGEKDATKLSDANNIGVVNNNGTLTVKLAKDIDLTKDGSVTI
                     GDTTVNNDGLTIKDGPSVTKAGINAGDKKITNVQDGEISATSKDAVNGSQLYQVQEVA
                     NAGWNLTANGKDKGNVKPGATVDLNNKDGN"
     gene            3628..>3828
                     /locus_tag="PWX02_002191"
     CDS             3628..>3828
                     /locus_tag="PWX02_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1730161.1"
                     /translation="MRKSRLSQYKQNKLIEQFVAGVTARTAYELVNVNKNTAAYYFHR
                     LRLLIYQHSPHLEMFEGEIEVDE"
BASE COUNT          973 a          896 c          615 g         1344 t
ORIGIN      
        1 gttaccgtct ttgttgttta gatcaactgt tgctcctggt ttaacgttgc ctttgtcttt
       61 accatttgct gttaagttcc aacctgcgtt agcaacttct tgcacttgat ataactggct
      121 accgtttacc gcatctttgc tggttgcaga aatttcaccg tcttgaacgt tagtgatttt
      181 cttatcgcct gcattgatac cggctttagt tacacttggg ccatctttga tagttaaacc
      241 atcattattt accgtagtat caccaatagt cacagaacca tctttcgtta aatcaatgtc
      301 tttcgctaat ttaaccgtta aagtaccgtt gttatttact acaccgatat tattagcatc
      361 tgagagctta gttgcatctt tctcaccacc ggtaatactt aacgtttcgt ttagtttctt
      421 agtaatgact ttaccatcat caccaacgaa ttttaagcca tcgtttaagg ttgctacttc
      481 ttctttaaca gggttgccgt ctttgtcttt ggtttcataa acgatacgag tcttcgtttc
      541 gccatcttta ccatcaacac ccggtttacc ttgctcgaaa gtcatagtgc cattcgaacc
      601 atctttaccg ttgataccga ttgtgccatc tttacccgga ccaccgatag ttaaatcgtt
      661 atttaatgca aaggttacat tgttgtcagt cgtttcttta gtaatgatga tattaccgtc
      721 ttcattatta aatgaaactt tacctttagg accaacattg gtgctgttat tgccatttgc
      781 tgcaatatcc caaccagagt tcgctacatc ataaacctgt tttaactggt cttctgttgc
      841 tgcttgacca ctagtaaagt tatttggatc aaatgtctta ttggttaagc caccgattgt
      901 acctttatta ccgtcaacag taatcgaatt atcgccacct actgttaagc tatcaccggt
      961 taatgccgaa tcacctacac caacaacatt cgtgtcaata taagtatcac cgattgttac
     1021 cgaaccttcg tctgttaagt caatgtttct tgctaatttc accattaact taccatcttt
     1081 attcacaacg ccgatattat catcagataa atcctctaaa tcggtatcgc cgcctgaaat
     1141 ttgtaaggtt tcatttagct tcttattgat aactttaccg tcatcaccta cgaatcttaa
     1201 gccatcgttc agtgttgcaa cttcctcagt tgtaccgtct ggtttctcat aaacgatacg
     1261 ggttttgctt tcgccatctt taccgtcgtt accgtcaagg cctttagcac cgtcttttac
     1321 agagatagtt gcacttgcac catctttacc gtcttgacct ttaggaccag ttaatccgat
     1381 tgagccgtct tttccgttta aggtcacacc agctgtacca tcggcacctt taacaccaac
     1441 cttaccatca acaccatctt taccgtcttt acccggacca ccaatggtta aattgttgtt
     1501 taaggcaaag tcgatcgcat tattacttac ggtagtgatg atgtttttat ctttagaagt
     1561 gtaatttacg gtttccccta atttcacatt atcttcttta gcaccatctt ccagttcaga
     1621 attatccgca gtaatgttaa agcctttgtt gattaagcta tccgttgcat tacgtacatc
     1681 tttaatgttc gctgcattag tacctacttt ttctagctgc tcatctgtta aatcattcac
     1741 agattcaaca gctttaccat ctttaccagg ctgaccatta ataccatcaa taccgctctt
     1801 cacgttaaca atttggttac caccattatt tagaccgtct ttggttaacg atactacatt
     1861 accgtcttta ccatcttttc cgtctttcgg tgttaccgtt aaaccattgt tgtttacaac
     1921 tgttttcggt gcatctgcat ttatagaacc gtcttctttg tattcaacaa agtttgctgt
     1981 agcaagatct tttaagtcac ggtttaactg aatttcaagc tgatctttct cattagcaac
     2041 aacattgata ttacctgctg ctgatttaaa tgcttcagaa gttgccttat caacaccttc
     2101 acccacaatt ttaactactg tgtttagctt gtgacggttt agtaactcat tattacctgt
     2161 aaagattaag ccatcgttta aggttgcaac agtttcagga tctccttgtg ggttaccgtc
     2221 tttatcagtc ggttggtaaa cgatacgtgt tgtgccattt tgacccggta agccgttttc
     2281 accatcttca ccattaacac cttgagcacc atccgctgat ttaaatgtta agccgtcttt
     2341 accgtctttt ccacctacat tgaggttatt cgctaagtcg aagtcgatct tgttatcgtg
     2401 taccgttgta acgatgttac cgctttcact acgatactct actgtttgac caagctctac
     2461 agtatcttct tttgcatcat ctgcaagttt agaattatca gcagtgatct tgaagcccgt
     2521 tttcactaga tcatcagtag cattacgtac atcattgata ttcgctgcat ttgtgccaat
     2581 ttctttaagc tgctcttctg ttaaatcatt tacagattta actggttgac catcttgacc
     2641 atcttgaccg ttaacaccat cgataccgct cttcacgtta acaatttggt tgttaccgtt
     2701 atctaaacct ttatctgtta atgatactgg tttagcatct gcgccatttt cactattagc
     2761 tggtgtaata gtgatacctt tattagtaag ctctgttgat ggtttagtat tatcaacaac
     2821 ctctttacca tcatcacctt gtttgtactc aacaaatttc gcatttgtta aatctttaag
     2881 ctcacggtta agttgaattt caagcttatc tgtaccatca gctttaacat tgatgttacc
     2941 tgctgctgat ttaaatacag ctgatgctgc tttatcaaca ccttcaccca caacattaac
     3001 tactgtgttt agcttatgac gatttaacgt atctgcattg ttaccagtaa agatcaagcc
     3061 atcatttaag ttagcaactt gctcttcttt atcgccattt ttgtaaacga tacgagtttc
     3121 gccgttatca cctggtaagc catctttacc agtgccatca acaccttgtt tgccatcttt
     3181 aacagcaata tttgtgcttg cacctggctg accatcagca cctttcgggc ctgttaagcc
     3241 gatagagcca tctttgccgt ttaatacaac agacgcaccg tctttaccat ttacgccaac
     3301 tttaccgtca ataccatcgg caccattacg acctacaaca actgatgctt cgcctggtac
     3361 gccgtcttta ctgtcatcac ctttaattga aacaccatct ttgccgtctt ggcctttaac
     3421 gccgattgta ccggcttcac ctggctgacc atctttaccg tctttaccaa cagataacgt
     3481 agaatttaag ctaaattgga tttcattatc tttaactgtt gtcacaatat tgctgtctgg
     3541 atcggtaaat ttaactgttt gacctaacta acattagggg ctgtcctaga taacgactaa
     3601 aaactccatt taggttaaga tacccaaatg agaaaaagtc gtctaagtca gtacaaacaa
     3661 aacaaactca ttgagcagtt tgttgcaggg gtaacagccc gaacagcata tgagttggtc
     3721 aatgtaaaca aaaacacggc ggcgtattac ttccatcgct tgcggttgct tatctatcaa
     3781 catagccctc atttggaaat gtttgaggga gaaattgagg ttgatgag
//
LOCUS       DANJWC010000185         3813 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.185, whole genome shotgun sequence.
ACCESSION   DANJWC010000185 DANJWC010000000
VERSION     DANJWC010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.185"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..814
                     /locus_tag="PWX02_002192"
     CDS             <1..814
                     /locus_tag="PWX02_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL1730162.1"
                     /translation="SYRLPRSLSAIATGAMLAVSGSLLQILTRNPMASPEVLGVSSGA
                     TIAAILSFLLFPTLSSIGFLLFGVLGSLAVLALIMWLSRKLQPSALMIAGVAIGALVS
                     GLMAIIQLSGSPRLTAVISWLSGSTYYANPNTVWILLSVAILLISATLLFIKPLRLIS
                     LGETVSLHLGLEVKKVQMIVLLLIALMSAASTLAVGPMSFIGLMTPHLAKNLGAITPE
                     KQIPISALLGAMLMLIADWLGRYLIFPYEIGAGIIASILGGIYFLLLLKQHK"
     gene            842..2974
                     /locus_tag="PWX02_002193"
     CDS             842..2974
                     /locus_tag="PWX02_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HDL1730163.1"
                     /translation="MNTLSKAIKLALYGSSIGSISTAYAETVTHLNTVEVSAETPNAS
                     SEETGRYAQKKAKGSNGMALSAKDTPQSVTAVTHQQIRDQNLNTIAKALEATHGVSVS
                     LVDRGRYSFSARGFGIDKVKVDGMDLKVNNQWTTGENTGNSVLYDRVEVVRGATGLTT
                     GAGDPSASVNLVRKRANSTSRYTILEAGIGRYADFNTMIDHSQALNNSGSVRGRFIAE
                     HKNGNTFIKNEKERYTTLYGNIEADLTDTTKLGLGVSYQKEKRDAALWGGLPAFYSDG
                     GETNWSRSKNASPEWAYWDNESVNYFADITQKLGEKWELALKGNYRVGKHNSELFYFS
                     GSSLNRQDGLGWSPWPGKFKNDNTQSNVQLDLTGSFSAWGLEHDAAIGIQYNRAHRNS
                     YAADNSTFDGAFDFNNWNGIYARPNWGSMSEKYYQNLKEFGVYVSTRLRLTDRLSMLL
                     GSRISSYRTEGTFYGSTQNFHANDVGTPYAGLTFDINPQNTVYVSYTDIFKPQSQVDI
                     NNKLLSPVTGETYEAGWKGSYFNDNLQTQISVFETRQDNLAQSAGINPNTLLAYYTAS
                     KGAKVRGFEVEASGNVTEKLKLNAGYTQWKGDDASGNPINTTQPRKQFKFFATYDMNR
                     LVQGLTVGAGVNWQSRIYTKHATYGEYGQKAYTLVNLMARYQFNDNFSAQLNIDNLFD
                     KKYRNALSFSQYSYGEPFYTRLNLRYEF"
     gene            3085..3729
                     /gene="adk"
                     /locus_tag="PWX02_002194"
     CDS             3085..3729
                     /gene="adk"
                     /locus_tag="PWX02_002194"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDL1730164.1"
                     /translation="MKVILLGAPGAGKGTQAQFIVNKFGIPQISTGDMFRAAIKEGTE
                     LGKQAKALMDEGKLVPDELTVALVKDRIAQPDCANGFLLDGFPRTIPQADALKEAGVK
                     IDLVLEFDVADEVIVERMSGRRVHQPSGRTYHVVYNQPKVEGKDDVTGEDLIIRQDDK
                     PETVLERLAIYHKQTKPLITYYTAEAEAGNTRYERLDGTKPVEEVSAELAKILS"
BASE COUNT         1172 a          751 c          805 g         1085 t
ORIGIN      
        1 ttcctaccgc ttgcctcgta gtctaagtgc gatagcaaca ggggcaatgc ttgcggtaag
       61 tggtagccta ttacaaatct taacgagaaa cccaatggca agccctgaag tattaggtgt
      121 gagttcaggg gcaactattg ctgcaatact gtcattctta cttttcccaa cattatctag
      181 cattggcttc ttgttatttg gtgtattagg gagtttggcc gttctcgctc ttattatgtg
      241 gctttcccgt aaattacaac catcagcgtt aatgattgca ggtgttgcta ttggggcttt
      301 agtcagtggc ttaatggcta ttattcaact aagcggcagc cctcgtttaa cggcagttat
      361 tagttggctg tctggctcaa cctattacgc taatccgaat acagtttgga ttttattatc
      421 tgttgcaatc ctacttatca gtgccacttt actgtttatc aaacctttac gcttgatctc
      481 acttggcgaa accgtttctc tccacttagg gttagaggtt aaaaaggtgc agatgattgt
      541 attactttta atcgccctaa tgagtgccgc ctctacgctt gctgtagggc caatgagctt
      601 cattgggcta atgacccctc atttagctaa aaatttaggt gcaattacac cggagaaaca
      661 gatccctata tcggcattat taggggcaat gctaatgctc attgccgatt ggctcgggcg
      721 ttatctaatt ttcccttatg agattggggc aggcattatt gcctctattc tcgggggtat
      781 ctatttctta ctgttactca aacaacataa ataatttttt attcattact taggagaaat
      841 tatgaacacg ctatctaaag cgataaaact tgccttatac ggttctagta tagggtctat
      901 ctcaacagct tatgctgaaa cagttactca tcttaatacc gttgaagtaa gtgccgaaac
      961 gccaaatgcc tcatcggaag aaactggacg ttatgcacag aaaaaagcca aaggctctaa
     1021 tggaatggca ttgagtgcga aagacacgcc acaaagcgta acggctgtaa cgcatcaaca
     1081 aatcagagat caaaatctta atacgattgc caaagcactt gaagcaactc acggtgtttc
     1141 tgtttcttta gttgacagag gacgctattc tttcagtgct cgtggttttg ggattgataa
     1201 agtcaaggta gatggtatgg atctcaaagt aaataaccaa tggacaacgg gcgaaaatac
     1261 aggcaacagc gtgttatacg accgagtaga agttgtgcgt ggtgctacag gcttaactac
     1321 tggggcgggc gatccctctg caagtgttaa tcttgttcgt aaacgagcca atagcacttc
     1381 ccgttatact attttagaag ctggaatcgg gcgttatgct gatttcaata caatgattga
     1441 ccatagccaa gcattgaata attcaggatc agtaagaggc cgctttattg cagagcataa
     1501 aaacggtaat acgttcatta aaaatgaaaa agaacgttac accacgcttt atggcaatat
     1561 tgaagcagac ttaactgaca ctaccaaact tgggttaggt gtaagttatc aaaaagaaaa
     1621 acgtgatgca gcactttggg gtggtttacc cgcattctat agcgatggtg gtgaaactaa
     1681 ctggagcaga agcaaaaatg ccagcccaga atgggcttac tgggataatg aatccgtaaa
     1741 ctattttgcc gatattacac aaaaactagg cgaaaaatgg gaacttgcat taaaaggcaa
     1801 ttaccgtgta ggcaaacata attcagaact tttctatttc agcggctcat cacttaatcg
     1861 acaagatgga ttaggttggt cgccttggcc gggtaaattt aaaaatgata atacacaatc
     1921 taatgtgcag cttgatctta ccggttcatt ctctgcttgg ggattagaac acgatgcggc
     1981 aatcggtatt caatacaatc gagcacatcg taattcctac gctgccgata acagcacctt
     2041 tgacggagct ttcgacttta ataattggaa tggcatttat gctagaccaa actggggaag
     2101 tatgtctgaa aaatactacc aaaacttaaa agagttcggg gtgtatgttt ctacccgttt
     2161 acgtcttaca gatagacttt ctatgcttct aggttctcgt atttcatcgt accgcacaga
     2221 aggcactttc tatggttcaa cacaaaactt ccacgctaac gatgttggga caccttatgc
     2281 aggattaacc tttgatatta atccacaaaa taccgtttat gtaagttata ccgatatttt
     2341 caaaccacaa agtcaagtgg acatcaataa caaactactc tctcctgtta cgggcgaaac
     2401 ctacgaagca ggttggaaag ggagttattt caatgacaac ttgcaaactc aaattagcgt
     2461 atttgaaaca agacaagata atctagctca atctgccggt attaacccta atacattatt
     2521 agcctactac acagcctcta aaggtgcgaa agttcgaggc tttgaagtag aagcatctgg
     2581 aaatgtaact gaaaaattaa aactcaatgc aggatatact cagtggaaag gcgatgatgc
     2641 ttcaggcaac ccaattaaca caactcaacc acgcaaacaa tttaaattct ttgcaactta
     2701 cgatatgaac cgtttagtgc aaggtttaac ggtaggagca ggtgtaaact ggcaaagcag
     2761 aatttatacc aaacacgcta cttatggcga atatggtcaa aaagcttaca ctttagtgaa
     2821 tttgatggct cgttatcaat ttaatgacaa cttctcagca cagcttaaca ttgataactt
     2881 attcgataaa aaatatcgta acgccctcag ctttagccaa tatagctacg gtgagccatt
     2941 ctatacacgc ttaaatttac gctatgaatt ctaattccgt ataacaagcg gtcaaaaaat
     3001 taaaaaaatt tgcaatttta ggcagtaatc ggtaaggtta ctgcctaagt tatttattca
     3061 tttttctagt tattgaggtt tacaatgaaa gttattttat taggtgcacc gggtgcgggc
     3121 aaaggtacgc aagctcagtt tattgtgaac aaattcggta ttccgcaaat ttcaaccggc
     3181 gatatgttcc gtgcggcgat taaagaaggc actgagcttg gtaaacaagc aaaagcattg
     3241 atggacgaag gtaaattagt gccggatgaa ttaacggttg ccttagtaaa agaccgtatc
     3301 gctcaacctg attgtgcgaa cggtttctta ttagacggtt tcccacgcac cattccgcaa
     3361 gctgatgcgt taaaagaggc tggcgtaaaa attgatttag tacttgagtt tgatgtagcc
     3421 gatgaagtga ttgttgagcg tatgagcggt cgtcgtgttc accaaccatc agggcgtact
     3481 taccacgtgg tgtacaatca accgaaagtg gaaggtaaag atgatgtgac tggtgaggat
     3541 ttaattatcc gccaagacga caaacctgaa accgtgttag agcgtttagc gatttaccac
     3601 aaacaaacca agccgttaat cacctactac accgcagaag cagaagccgg caatactcgt
     3661 tatgagcgtt tagacggtac aaaaccggta gaagaagtaa gcgctgagtt agcgaaaatt
     3721 ttatcttaaa cggattatta aaatgaacaa gcggtaatat ttttcgagaa tattaccgct
     3781 tgttatttgg cttaaacaca atatttggtg agc
//
LOCUS       DANJWC010000186         3802 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.186, whole genome shotgun sequence.
ACCESSION   DANJWC010000186 DANJWC010000000
VERSION     DANJWC010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.186"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..601)
                     /locus_tag="PWX02_002195"
     CDS             complement(<1..601)
                     /locus_tag="PWX02_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HDL1730165.1"
                     /translation="MKLTKIALSLTALVVSSMSVAGTLSTSSMVDILAFDGQRVKSGT
                     TSVQMSENKTHQVVVEVGGTVEGDFFTSDQIILTFQGSAENAKLETPRLNSKLDLRKF
                     QENQTVAIKTDSGKVISHKQDFLKGEGFLAKTRVEDNLTKYNLAKKTASVEQFATAPL
                     EAKGQIVVDTNKVSQEELQLLFRKADKDTQKRFLEWAKKN"
     gene            739..1638
                     /gene="corC"
                     /locus_tag="PWX02_002196"
     CDS             739..1638
                     /gene="corC"
                     /locus_tag="PWX02_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249295.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HDL1730166.1"
                     /translation="MSDEQQSVNTTEKSEKKSILHSLFSGLFQNEPKNREDLVEVIRD
                     SVENELIDSDTKEMIEGVMEISELRVRDIMIPRAQIVFIDANEPLDVCIDLIIESAHS
                     RFPVIRGEKDTIEGILHSKDLLKYLRSDSEPFNLQQVLRPAVIVPESKRLDRMLKEFR
                     SERFHMAVVVDEFGAVSGLVTIEDILEQIVGDIEDEFDEEEVKPIRQLSRHTYAVLPL
                     TDIKKFNEFFATDFNDEEVDTIGGLVMQAFGYFPKRDEQIELNGIDFKVTSANSRQLI
                     QLRVTLSDEQLEKMEKTSPNEEE"
     gene            1697..3205
                     /gene="lnt"
                     /locus_tag="PWX02_002197"
     CDS             1697..3205
                     /gene="lnt"
                     /locus_tag="PWX02_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HDL1730167.1"
                     /translation="MNFAKNPPFLTACLLAIILGAIGTFAYSPFDIWILAFASAAGLV
                     WSATLANRKTALWATFAWSIGYFCTGVNWVSVSMTQFGGVPTIVSYIAVFLLACYLAI
                     YNLLFSVVSHRLKLNNPFALAAIFTFTEYLRGVVFTGFPWLQFGYSLIDSPFAGIAPI
                     LGVEGLTFLVIVASSYLVKIIQQENKTVPSLMVLAVIFGLSFATRFLQFVQIDTEKQP
                     LVVSLVQGNIEQKMKWDPQHFNHSVRTYEQLILPLLGNSELIILPESAIPALENQISP
                     LLGSLDHLARQHGSEIIIGTLYQNEQEELFNSSVLLGEKEREYALHSNPRYNKHHLVP
                     FGEYVPFGSVLDWMREVFILPINLSQGSFVQPSFLAKNNRFNMAICYEIIFGDQLQQN
                     QKSQNSDYLLTITNDAWFGSSIGPWQHFQMARMRALELGKPLLRAANTGITAIVDANG
                     KVIEQLPQFTADALTATIVPTKGDTPFKQLGHWLIYSFSLFCVIASLLLRRK"
     gene            complement(3251..3715)
                     /locus_tag="PWX02_002198"
     CDS             complement(3251..3715)
                     /locus_tag="PWX02_002198"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDL1730168.1"
                     /translation="MATFQGNYIATGLKFGIVATNWHKIFIDQLLNGATDKLLRHGVE
                     EKNIDTVWVPGALEISIAAKKMAQSGRYDAIICLGAVVRGATSHYDVVVNESAKGISS
                     SALETGVPIINGILTVENLEQAIERSGTKAGNKGEECAMVAIEMANLLKALA"
BASE COUNT         1056 a          800 c          747 g         1199 t
ORIGIN      
        1 tatttttctt cgcccattct aagaaacgtt tttgggtgtc tttatctgcc tttctaaata
       61 aaagctgtaa ttcttcttgt gaaactttat ttgtatccac cacaatttgt ccttttgctt
      121 caagaggtgc cgtcgcaaac tgttcaactg aagctgtttt tttcgctaag ttatatttcg
      181 ttaaattatc ttcaactctt gttttcgcaa ggaaaccttc cccttttaag aaatcttgtt
      241 tatgtgaaat aaccttacct gaatcggttt taattgccac agtctgattc tcttggaatt
      301 tacgaagatc taactttgaa tttaatcttg gcgtttctaa ttttgcattt tccgcactgc
      361 cttggaaagt caaaatgatt tgatcagagg tgaaaaaatc cccttcaact gtcccaccga
      421 cttccactac cacttgatgc gttttattct cactcatttg aaccgaagtc gtgccggatt
      481 taacacgttg cccgtcaaag gctaaaatgt ccaccattga tgatgtactt aacgtcccgg
      541 ccacactcat cgaactgaca actaaggctg ttaaacttaa tgctattttg gttaatttca
      601 tttcaaactc ctaaaatatg aagaaataaa aagatttttg cttatcctat gctaatttga
      661 gtgaaaatag caatgtttta tttgctttgt gactgaattt gcttattata agcgtacttt
      721 atttaagtgg aacaaactat gagtgatgaa cagcaaagtg taaacaccac cgaaaaatca
      781 gaaaaaaaat cgattttaca ctctctattt agcgggcttt ttcaaaacga gcctaaaaac
      841 cgtgaagatc tagtcgaagt gattcgagac tctgttgaaa atgaattgat cgacagcgac
      901 accaaagaga tgatcgaagg agtaatggaa atctcggaat tacgagtgcg ggatattatg
      961 attccccgtg cgcaaattgt gtttattgat gccaatgaac ctttagatgt ctgtattgat
     1021 ttaattattg aatccgcaca ctctcgtttt ccggtgattc ggggtgagaa agatactatc
     1081 gaaggtattc ttcactctaa agatttattg aaatatttac gttctgactc tgaacccttt
     1141 aatctgcaac aggtattacg ccctgcggtt attgtaccgg aaagcaaacg cctagaccga
     1201 atgctaaaag agttccgttc agaacgcttc catatggcag tagtggttga tgaatttggt
     1261 gcggtttcag gcttagtcac cattgaagac attcttgaac aaattgtggg tgatattgaa
     1321 gatgagtttg atgaagaaga ggtcaaacca attcgccagc tttcaagaca tacttatgcg
     1381 gtattaccgc tcaccgacat caaaaaattc aatgaatttt ttgctaccga ctttaatgac
     1441 gaagaggtgg ataccattgg tggtttagtg atgcaggctt tcggttattt cccgaaacga
     1501 gatgagcaaa ttgagttaaa cggaattgat tttaaagtca cctcagctaa tagccgccaa
     1561 ttgattcagc tacgagtcac actttccgat gaacagttag agaaaatgga aaaaacctct
     1621 cctaacgaag aagaataata tagtgagttt caatcaaggc acgtttcggc gtgcctcatt
     1681 tatttagtga gttattatga attttgcaaa aaatccgcct tttttaaccg cttgtctcct
     1741 tgcaatcata ctgggtgcaa tcggcacttt cgcctattcg ccttttgata tttggattct
     1801 tgcctttgct tccgcagccg gcttggtttg gtcagccact ctggcaaaca gaaaaaccgc
     1861 attatgggca acctttgctt ggtctatcgg ctacttctgc accggtgtaa actgggtttc
     1921 tgtgagtatg acccaatttg gtggtgttcc taccatagta agctacatag cagtattcct
     1981 gttggcgtgc tatctcgcta tttataatct gctgtttagt gtagtttcac acaggctcaa
     2041 actgaataat ccgtttgcat tggctgcgat ttttaccttt accgaatatt tacgaggcgt
     2101 ggtatttacc ggttttccgt ggctacaatt cggctatagc ttaatagaca gcccctttgc
     2161 tggtattgcg ccgattttgg gcgttgaagg tttgactttc ttggtaatag ttgcaagtag
     2221 ctatctagtg aaaattattc agcaagagaa caaaactgtg ccatcattga tggtactagc
     2281 ggtcattttt ggcttaagtt ttgccactcg ctttctccaa tttgtgcaaa ttgatacaga
     2341 aaaacaaccg cttgtagtga gtttagtaca agggaatatt gagcaaaaaa tgaagtggga
     2401 tccgcaacat ttcaaccaca gcgtacgaac ctacgaacag ctcattctgc ccctattagg
     2461 taacagtgaa ctgattattt tgcctgaatc cgccattccg gcacttgaaa atcaaatcag
     2521 cccgctgctt ggctcgctcg atcacttagc tcgccaacac ggaagtgaaa ttattatcgg
     2581 cacactctat caaaatgagc aagaggaact gtttaacagt agcgtgctat taggcgaaaa
     2641 agagagggaa tatgccctgc acagcaaccc tcgctacaac aaacatcacc ttgtgccgtt
     2701 tggcgaatat gtgccgtttg gctcggtgct ggattggatg cgggaagtgt ttattctacc
     2761 aattaatctt tcacaaggca gtttcgttca accatcattc ttggcgaaaa acaaccgatt
     2821 taatatggcg atttgctatg aaattatttt tggtgatcag ttacaacaaa accaaaaaag
     2881 ccaaaattca gattatttat taactatcac caatgatgcg tggtttggtt cttccatcgg
     2941 gccttggcaa catttccaaa tggcaagaat gcgagcgtta gaacttggca aaccattgct
     3001 tcgagctgcg aataccggta ttaccgccat tgttgatgcc aacggaaaag taattgaaca
     3061 actaccgcaa tttactgctg atgcactaac tgccaccatt gtgccaacca aaggcgatac
     3121 gccgtttaaa caattaggtc actggttaat ttacagtttc agcctatttt gtgtcattgc
     3181 cagcctgtta ctacgcagaa aataaaaaaa tccccttagg ctcttgggtc taaggggatt
     3241 ttatcgctga ttacgctaag gcttttaata ggttcgccat ttcaatcgct accattgcac
     3301 actcttcgcc tttattaccg gctttagtgc ctgaacgctc aatcgcttgc tctaaatttt
     3361 ccaccgttaa aataccgtta ataatcggca cgccggtttc taaagcagaa gagctgatgc
     3421 cttttgctga ctcgttcact accacatcat agtggctggt tgctccacgc acgaccgcac
     3481 ctaagcagat aatcgcatca taacgaccgc tttgtgccat ttttttcgct gcaattgaga
     3541 tttctaatgc acccggcacc catacggtgt cgatattttt ttcttctacg ccgtgacgca
     3601 ataatttatc ggttgcaccg tttaacagtt gatcgataaa aatcttgtgc cagttagttg
     3661 ccacaatacc gaattttaaa ccagttgcga tgtagtttcc ttgaaatgtt gccattgttt
     3721 gttttccttc tttaaaataa atttgatact caataaccct ctccccttgt gggagaggga
     3781 tagatttttc ttcgttcaga ag
//
LOCUS       DANJWC010000187         3800 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.187, whole genome shotgun sequence.
ACCESSION   DANJWC010000187 DANJWC010000000
VERSION     DANJWC010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.187"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            109..540
                     /locus_tag="PWX02_002199"
     CDS             109..540
                     /locus_tag="PWX02_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HDL1730169.1"
                     /translation="MSEFGSVEHYLPESVKEIVDLIGLSATEKLIKAFGGFSFQFSKG
                     KVYFEKLKDVLGEEDAEKLYDYLRSNIAYLPRCQTALRLLRNERIYTDYCRMTEQEGI
                     SGRLAIMQICSKYGVSDRIAWDAVRYLKSKPKQPITQTSLF"
     gene            626..1159
                     /locus_tag="PWX02_002200"
     CDS             626..1159
                     /locus_tag="PWX02_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HDL1730170.1"
                     /translation="MTEPIKNKATIADFNLAFDRLIGHEGGYVNDPKDPGGETNWGIT
                     IHTARANGYTGSMRAMSRADAKTIYLKAFWQRYNCEQFPPELAFQFLDACVNHGSGNA
                     SRMLQRAVGVADDGIIGKVTLAAIERYSVADVGLLFQAERLEFYTKLKHFDRFGRGWI
                     NRMAGNLRYFAKDTVEE"
     gene            1162..1404
                     /locus_tag="PWX02_002201"
     CDS             1162..1404
                     /locus_tag="PWX02_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2644 domain-containing protein"
                     /protein_id="HDL1730171.1"
                     /translation="MKQFLLDLITNKDGTASTTGFIQFISWLVLSGILIHAYLYDKPF
                     ISDWWFAYAGICVLGSPATKGVVSALKKDRTKGDEE"
     gene            1401..1760
                     /locus_tag="PWX02_002202"
     CDS             1401..1760
                     /locus_tag="PWX02_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2681 domain-containing protein"
                     /protein_id="HDL1730172.1"
                     /translation="MITQQIVGFIGVIGTVIAYAFFKGWQLKQERKLTATLKAEKQQQ
                     AVEIQQKNAEVKNAQIQQKHINETRRIQPSAVDERLHQHGYFRDDYGLHGVRSDLSEP
                     CGHNGNETPSACTQSDL"
     gene            1775..1930
                     /locus_tag="PWX02_002203"
     CDS             1775..1930
                     /locus_tag="PWX02_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730173.1"
                     /translation="MKLIKTLRQRWQQWRFYRRRPDLAELHNVVRAAAAQGNQHPVGR
                     AKRGRYD"
     gene            1923..2180
                     /locus_tag="PWX02_002204"
     CDS             1923..2180
                     /locus_tag="PWX02_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730174.1"
                     /translation="MIDEKLFQMLISFVVAPLVAFVGKVIFDRINRNEQTVKELKAEM
                     ESKYQSKELAHEVNRGIKEKLDDILDSLKEVNQKLDKKADK"
     gene            2180..2434
                     /locus_tag="PWX02_002205"
     CDS             2180..2434
                     /locus_tag="PWX02_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730175.1"
                     /translation="MSRKKQKQTDSEKLDLILGGLNELNDKVDKQNEEIERLHREIQA
                     TNRLVNEMARKNRRDAVVAGGLGGGIVAVGFELLRLKFGM"
     gene            2442..2942
                     /locus_tag="PWX02_002206"
     CDS             2442..2942
                     /locus_tag="PWX02_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HDL1730176.1"
                     /translation="MAHDVEVQKAVRQAYVFDRLSLEMAAERAGVSFGTARRWKANAE
                     KNGDNWEKARDVQAMASGGIENIAQGLLAGFLIKYRTLMTELEENTKMTTEAKVEALA
                     ALADSFAKMTASSKKLLPETSAMATAMRAIEMMANIVKTKKPHLLPDFLEMLDDLEVQ
                     FKKEFK"
     gene            2942..3088
                     /locus_tag="PWX02_002207"
     CDS             2942..3088
                     /locus_tag="PWX02_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730177.1"
                     /translation="MAKKKQTVVHKHYSFRWIVQLACILGAIKLMLNGTDGWGWLLLI
                     AVCL"
     gene            3092..>3800
                     /locus_tag="PWX02_002208"
     CDS             3092..>3800
                     /locus_tag="PWX02_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730178.1"
                     /translation="MKIKEFEKELEELRRQLQQNIEASFEGWDDKPQAISERRQKVLD
                     PVHGFEYFVQAYFPHYVRSEHKSQLHHYLFENLPLSVSDLSKSVRQAIAAPRGEAKST
                     ICTQLFPLWCLVRDLKRYIIIAMDTKEQAYSMLEAIKVEIESNPRLRIDFPEVAQGKV
                     WRAGVIMTAKKQKVEAVGAGQKLRGRRHGAYRPDLVVLDDIENDESVEKPAQRNKLHN
                     WVLKAVLKLGAAGEKFDV"
BASE COUNT         1134 a          703 c          935 g         1028 t
ORIGIN      
        1 cggtcttttt ttatgcaaaa tttgcaaaaa aatttttaag taagtattac agatttgaat
       61 aacccccttg gataatggct tttgtggaaa ttattcaagg ggatttttat gtctgaattt
      121 ggaagtgttg agcattattt gcctgaatct gtaaaggaaa ttgttgattt gattggcttg
      181 tctgcaaccg agaaactgat taaagccttt ggaggctttt cgttccagtt ttccaagggg
      241 aaggtttatt ttgaaaaact taaagatgtg ttaggagaag aagatgcaga aaaattatac
      301 gattacttaa gatctaatat agcttatttg cctcgttgcc aaacagcctt gcgattattg
      361 cgtaatgagc gaatttatac cgattattgc cgaatgacag aacaagaggg aataagcggt
      421 cgtttggcaa ttatgcagat ttgctcaaaa tacggtgtgt ccgaccgtat tgcgtgggat
      481 gccgtaaggt atttaaaatc taaacctaag caacctatta cgcaaacatc acttttttaa
      541 tttgtggaac tctttcatct cccctatctt acattaaatc attaaagtct ctttaaatca
      601 tcattgaatt taaagaggct tttttatgac tgaacctatc aaaaacaagg caactattgc
      661 tgactttaac cttgcatttg accgtcttat cggacacgag ggcggttatg tgaatgaccc
      721 gaaagacccc ggtggcgaaa cgaattgggg tatcactatc catactgccc gtgcgaatgg
      781 ctataccggc tctatgcgtg caatgagccg tgcggatgcg aaaactattt acctcaaagc
      841 cttttggcaa cgttataact gtgagcagtt cccgcctgag ttggcttttc agtttttaga
      901 tgcgtgtgta aaccacggtt cgggcaatgc tagccgaatg ttacagcgag cagtaggtgt
      961 ggctgatgac ggcattatcg gcaaagtgac tttggctgcg attgagcgtt attcagtggc
     1021 tgatgtgggc ttgcttttcc aagcggaacg ccttgagttt tacaccaagc tcaaacactt
     1081 tgaccgtttt ggtagaggtt ggattaaccg aatggcgggc aatttgcgtt attttgccaa
     1141 agacacggtg gaggaataat cgtgaaacaa tttttacttg atttaattac caataaagac
     1201 ggcacagcca gcacgactgg ttttattcag tttatcagtt ggctggtgct gtcgggcatt
     1261 ttaattcacg cctaccttta cgataagccg tttatttccg actggtggtt tgcttatgcc
     1321 ggtatttgtg tgctgggcag ccctgccaca aaaggcgtgg taagtgcgtt aaaaaaagac
     1381 cgaacaaagg gagatgaaga atgattactc aacaaattgt cggttttatc ggtgtgattg
     1441 gcacagtaat tgcctatgcg ttttttaaag gctggcaatt aaagcaggag cggaaactta
     1501 ccgctacatt aaaggcggaa aagcagcaac aagcggtcga aattcaacaa aaaaatgcag
     1561 aggttaaaaa tgctcaaatt caacaaaaac acattaacga aactcgccgt attcagccta
     1621 gtgccgttga tgagcggttg caccaacacg gttacttccg tgacgactac gggttgcacg
     1681 gcgttcggtc tgatttatcc gagccgtgcg gacacaatgg aaacgaaacg ccaagtgctt
     1741 gcacacaatc tgacctatga ggaagtttgc cagaatgaag ttaattaaaa cactcagaca
     1801 gcgttggcaa caatggcgtt tttaccgccg ccgacctgac cttgcagaat tgcataacgt
     1861 ggtgcgtgcg gcggcagcac aaggtaatca acacccggtc gggcgagcca aaagggggcg
     1921 ttatgattga tgaaaaactg tttcagatgc tgatctcttt tgtagttgct ccgttagtgg
     1981 cgtttgtagg caaggtcata tttgatcgta tcaaccgcaa tgagcaaacg gtaaaagagc
     2041 taaaagcaga aatggagagc aaatatcaat ccaaagagct tgcccacgaa gtgaatagag
     2101 ggattaagga aaagctagac gatattttag actcgctcaa agaagtaaac caaaaacttg
     2161 ataaaaaggc ggataagtaa tgagccgtaa aaaacaaaag caaaccgatt cggaaaaatt
     2221 agacctgatt ttaggtggat taaatgagct taacgataag gtggataaac aaaacgaaga
     2281 aatcgaacgc ttacaccgtg aaatccaagc gactaatcgt ttagtgaatg aaatggcacg
     2341 caaaaaccgc cgtgatgccg tggttgccgg tggcttgggt ggcggcattg ttgctgtggg
     2401 ttttgagttg ttacgcctga aatttggaat gtagagggcg tatggcacac gatgttgaag
     2461 tacaaaaagc ggttaggcaa gcctatgtgt ttgaccgttt aagcctcgaa atggcagccg
     2521 agcgtgccgg tgtgtcgttt ggcacagctc gccgttggaa agccaacgct gaaaagaacg
     2581 gcgataactg ggaaaaagcc cgtgatgtgc aagcaatggc aagtggtggc attgagaata
     2641 tcgctcaagg cttgttagcc ggttttttaa ttaagtaccg cacgttgatg acggagcttg
     2701 aggaaaacac gaaaatgacc actgaggcga aagtggaagc attagctgct ctggctgatt
     2761 cgtttgcgaa gatgacggca tcgagcaaga aactattgcc tgaaacaagt gcaatggcaa
     2821 ccgctatgcg tgcgattgag atgatggcga atatcgtcaa aaccaagaaa ccgcatttat
     2881 tacctgactt tttagaaatg ctggacgatt tagaagtgca gtttaaaaag gagtttaagt
     2941 aatggctaag aaaaaacaaa ctgtggtgca taagcattac tctttccgtt ggattgtcca
     3001 acttgcttgt attctcggag caataaaact aatgctcaac ggtacagacg gttggggctg
     3061 gttgttgctt atcgcagttt gtttgtaatt tatgaaaatc aaagaatttg aaaaagaact
     3121 ggaagaatta cggcgacagt tacaacaaaa catcgaagcg agttttgagg gttgggacga
     3181 taaaccacaa gccattagcg aacgccgcca aaaggtttta gaccctgttc acggctttga
     3241 gtattttgtg caggcttatt ttccacacta tgtgcggtct gaacataaat cacagttgca
     3301 tcattatctc tttgagaatt taccgctttc ggtaagtgat ctttctaagt cagtccgcca
     3361 agcgattgca gcccctcgtg gcgaggcaaa atcaacgatt tgtacgcaat tattcccgct
     3421 gtggtgttta gtccgtgatt taaaaagata catcattatt gcgatggaca ccaaagaaca
     3481 ggcatacagt atgcttgaag ctattaaagt ggaaattgaa tctaaccctc gtttaagaat
     3541 tgatttcccg gaggttgcac aaggcaaggt ttggcgtgca ggtgtcatta tgacggctaa
     3601 aaaacaaaaa gttgaagctg tgggggctgg gcaaaaattg cgtggtcgtc gccacggagc
     3661 ttatcgtccg gatttagtag tgcttgatga tattgagaat gatgaaagcg tagaaaaacc
     3721 tgcacagcgt aataaattgc ataactgggt tttaaaagca gttttaaaac ttggtgctgc
     3781 aggtgaaaag tttgatgtaa
//
LOCUS       DANJWC010000188         3777 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.188, whole genome shotgun sequence.
ACCESSION   DANJWC010000188 DANJWC010000000
VERSION     DANJWC010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.188"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            154..2130
                     /gene="cpdB"
                     /locus_tag="PWX02_002209"
     CDS             154..2130
                     /gene="cpdB"
                     /locus_tag="PWX02_002209"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HDL1730179.1"
                     /translation="MLNRRKFIQLGATAMLVLGTQPSAFAQTAKNKAGLRVIATTDIH
                     GFLTDFDYYKDAPTEKFGFTRTASLIKQARAEVKNSVLVDNGDLIQGNPISDYQSTKG
                     AKEGKPEPSIMALNAMQYEVGTLGNHEFNYGLSYLDNAIKQAKFPIVNANIVKPGTDE
                     PFFTPYVIQQKEIVDEKGNKHTIKIGYIGFVPPQVMIWDKANLEGKVEARDIVKTAQK
                     YVPIVKKEGADIVVALAHTGPSDEPYKEGAENCAFYLADVKGIDAVIFGHSHRLFPNK
                     EFANSPNADIAKGTMKGVPESMAGYWGNNISVIDLSLAENDGKWGVVDGRAELRPIYN
                     TEEKKATVENHPEIVALLKETHEATRQFVSQPIGKATDNMYSYLALVQDDPTIQIVNQ
                     AQKAYVENVVKGLPELAGLPILSAGAPFKAGGRKNDPTGYTEVDKGELTFRNAADLYL
                     YPNTLVVVKATGAELKEWLECSAGMFKQIDVASDKPQYLLDWTGFRTYNYDVIDGVSY
                     QFDITQPARYDGECKLINPNSHRVVNLTFDGKPVDPKQEFLIATNNYRAYGAKFPGTG
                     DKHIVFAAPDENRQVLANYITTESKAHGQVSPKADNNWRIAPISSNAKLDIRFETSPT
                     DKAKAFIKENAQYPMEYIENDETGFAVYRIDLTK"
     gene            complement(2203..3681)
                     /locus_tag="PWX02_002210"
     CDS             complement(2203..3681)
                     /locus_tag="PWX02_002210"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDL1730180.1"
                     /translation="MDSILGKKINSTAIPSLLCDFYKTSHRIQYPEGSQFLYSTFTPR
                     SNIYAPFVTRVVSFGFQAFILKYLIHYFNDNFFSRSQHDVVSEYTQFISDTLNIEDSG
                     EHIAQLHQLGYLPIRIKAIPEGKSVAVKVPLMTIENTHSSFFWLTNYLETLINVSLWQ
                     PITSASIAFAYRKALAEFSEQTCDDNSHLPFQAHDFSMRGMSSLESAESSGAGHLTSF
                     LGTDTIPAISFVTAYYGSQHLIGTSIPASEHSVMSSHGVDELPTFRYLMAKYPNNMLS
                     IVADTTDFWHNITVNLPLLKQEILARPESAKLVIRPDSGDFFTIICGNSSSPVEHERK
                     GLIECLWDIFGGSINQKGYKVLNPHIGAIYGDGVTYDKMIRILEGLTAKGFASSNIVF
                     GVGAQTYQRNTRDTLGFAIKATSITINGVEKAIFKAPKTDNGLKKSQKGRVKVISYDH
                     YVDELTALDDFSDDLLEVVFENGKLVKSTNFDEIRQNIAQQF"
BASE COUNT         1281 a          696 c          742 g         1058 t
ORIGIN      
        1 ctcttaaaat aaatgcttga actcaaagaa agataaaaat gtgtttaaaa tttgatataa
       61 caagctataa atgtgaaaaa atatggcgaa caagcggtta tttttctaaa atattttaca
      121 atttaccctt tcatttcatt aaggagatat tttatgttaa atagaagaaa atttatccag
      181 cttggtgcaa ctgcaatgct tgtattgggg acacaaccgt ctgcatttgc acaaacagcc
      241 aaaaataaag ctggcttgcg tgtcatcgcc actacggata ttcacggttt cttaactgat
      301 tttgattatt acaaagatgc tccaacagaa aagtttggtt ttacccgtac ggcaagccta
      361 attaaacaag ctagagcaga agttaaaaac agtgtgttag tcgataatgg tgacttaatt
      421 caaggtaatc ctatttctga ctaccagtca accaaaggtg caaaagaggg caaacctgaa
      481 ccttcgatta tggcattaaa tgcgatgcaa tatgaagtcg gaacgctggg aaatcatgaa
      541 tttaactacg gtttaagcta tttagataat gctattaagc aagcaaaatt ccctattgtc
      601 aatgcaaaca ttgtgaagcc ggggacagat gaaccattct ttacacctta tgttattcaa
      661 caaaaagaaa ttgtagatga aaaagggaat aagcatacca ttaagattgg ttatatcggt
      721 tttgtaccac cacaagtgat gatttgggat aaagctaatc ttgaaggaaa agttgaagct
      781 cgagatattg tgaagaccgc acaaaaatat gtgcctatag taaaaaaaga aggggcagat
      841 attgtcgttg cattggcaca taccggccct tcagacgaac cctataaaga aggcgctgag
      901 aactgtgcgt tttatctagc tgatgtaaaa ggtattgatg cagttatttt cgggcattca
      961 catcgcctgt tcccaaataa agaatttgca aattcaccaa atgccgatat tgcaaaagga
     1021 acaatgaaag gcgtaccgga aagtatggct ggctactggg gaaataacat cagtgtgatt
     1081 gatttaagct tggccgaaaa cgatggtaaa tggggcgttg tggacggtag agcagagttg
     1141 cgtccaattt ataatacgga agagaaaaaa gcaaccgttg agaaccaccc tgaaatcgtt
     1201 gcattattaa aagaaaccca tgaagcaacc cgtcaatttg tgtctcaacc tatcggtaaa
     1261 gcaactgata atatgtatag ttaccttgcg ttagttcaag atgaccctac cattcaaatt
     1321 gtgaaccaag ctcaaaaagc gtatgtagaa aatgtggtaa aaggcttgcc ggagcttgca
     1381 ggattaccaa ttctaagtgc cggagcaccg tttaaagccg gtggacgtaa aaatgacccg
     1441 acaggctata ccgaagtcga taaaggcgaa ttaactttcc gcaatgcggc agacttatac
     1501 ctttacccaa ataccttagt ggtggttaaa gcaacaggag cagaattaaa agagtggtta
     1561 gagtgcagtg caggaatgtt caaacaaatt gatgtcgctt cggataaacc gcaatattta
     1621 ctggattgga caggtttccg cacctataac tacgatgtaa ttgacggtgt cagctatcaa
     1681 tttgatataa cccaaccggc tcgttatgac ggcgagtgca aattgatcaa ccctaattcg
     1741 caccgtgtgg tgaacttaac ctttgacggc aaaccggttg atcctaaaca agaattctta
     1801 attgccacca acaattaccg tgcttatgga gcaaaattcc cgggaacagg cgataaacat
     1861 attgtgttcg ccgcaccgga cgaaaaccgc caagtgttag cgaattatat taccaccgaa
     1921 agtaaagctc acgggcaggt aagtccgaaa gcggataaca actggcgtat tgcaccaatt
     1981 agctcaaatg caaaactgga tattcgtttt gaaacctctc caacagataa agctaaagct
     2041 ttcattaagg aaaatgcaca atatccgatg gagtatatcg aaaacgatga aaccggcttc
     2101 gcagtttacc gtattgattt aaccaaataa tcagagctaa atacaagcgg tctaatttgc
     2161 aaaaaaatta acaaatttga ccgcttgttt cattccattt ttctaaaatt gttgtgctat
     2221 attttgccga atttcatcaa aattggtcga tttgaccaac ttaccatttt caaaaaccac
     2281 ctctaaaaga tcatctgaaa aatcatccag tgcggttagc tcgtcaacat aatgatcata
     2341 agagattact tttactcgcc ctttttgcga tttttttaag ccgttatcgg ttttaggagc
     2401 tttgaaaatt gccttttcga caccgttaat tgtaattgaa gtcgctttga ttgcaaaacc
     2461 cagcgtatca cgagtattgc gttgataagt ttgagcccca acgccaaaca caatattgct
     2521 ggaagcaaaa ccttttgccg ttaagccctc taaaatccgt atcattttat cataagtgac
     2581 gccgtcacca taaattgccc caatatgcgg attaagcact ttataacctt tttggttaat
     2641 actaccacca aaaatatccc acaaacactc aatcagacct ttgcgttcgt gttcaacagg
     2701 ggaggaactg ttaccgcaaa taattgtaaa aaaatcaccg ctatcagggc ggataaccaa
     2761 ttttgctgat tccggtcgag ctaaaatttc ttgttttaat aatggcaaat taacggtgat
     2821 gttatgccag aaatcggttg tatctgccac aatagatagc atattatttg gatattttgc
     2881 cattaaataa cggaaagtcg gcagctcatc aacaccgtgt gaactcatca ccgaatgttc
     2941 tgatgccgga atagatgtac cgattaaatg ttgagaacca taataggctg tcacgaatga
     3001 aattgcagga atggtatctg tgcctaaaaa tgaggttaga tgcccggcac cggagctttc
     3061 tgctgattct aaggaactca ttccacgcat agagaaatcg tgtgcctgga agggtagatg
     3121 gctattatca tcacaggttt gctctgaaaa ttcggctaga gccttacgat aagcaaatgc
     3181 aatggaagct gatgtaatag gctgccataa tgacacatta atcagggttt ccaaataatt
     3241 agtcagccaa aaaaagcttg agtgagtgtt ttcaattgtc attaaaggaa ctttcactgc
     3301 aacagatttt ccttctggaa tagctttaat tctgataggt aagtaaccga gctgatgcaa
     3361 ttgtgcaata tgttcgccag agtcctcaat attcaatgtg tcagaaataa attgagtata
     3421 ttcagaaaca acgtcatgtt gtgagcgaga aaaaaaatta tcgttaaaat aatgaatcaa
     3481 gtattttaaa ataaatgcct gaaagccaaa tgatacaaca cgtgtaacaa atggagcata
     3541 aatattacta cgaggtgtga atgtactata taaaaactga gaaccttcag gatattgaat
     3601 tcgatgtgaa gttttataaa aatcacataa tagtgatggt attgcagttg aattaatttt
     3661 tttacccaaa atactatcca ttttcttaaa gtccttattt aacttttaag gctaaaggtt
     3721 atcataactt accataaaat gaaaagtatt tgttacaaaa tgagtgtatt tacataa
//
LOCUS       DANJWC010000189         3712 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.189, whole genome shotgun sequence.
ACCESSION   DANJWC010000189 DANJWC010000000
VERSION     DANJWC010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.189"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..257)
                     /locus_tag="PWX02_002211"
     CDS             complement(<1..257)
                     /locus_tag="PWX02_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1730181.1"
                     /translation="MSFYNDLMESLTAMKEHLEGKRTLRTETLSRPEPLQISADEVKA
                     IRAKLNLSQAVFAKRLRTSVKTYQGWEQGKSTPNPQATILLR"
     gene            complement(424..987)
                     /locus_tag="PWX02_002212"
     CDS             complement(424..987)
                     /locus_tag="PWX02_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL1730182.1"
                     /translation="MNAVFMTTNNTPILFTENSGGTERRRVIFKFDRIVPIEERDFNL
                     IDKIQAETGGIIRLLFDTFPEPLEAKALLEKQRVSQEALAVKMEVNHVLEFAQEFEIL
                     PTVNGLAMGSSLNNGTRDSAIYPAYIYFCQLNDIEPINRRTFTRAFKQALKELDKGEY
                     QTRESNGRTVTNVHYIDKLRTFNKWQG"
     gene            complement(1243..1656)
                     /locus_tag="PWX02_002213"
     CDS             complement(1243..1656)
                     /locus_tag="PWX02_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730183.1"
                     /translation="MKHELNLAGITALMSEPIKATLSFLDFPHIRIERLYHDNGRPFF
                     IGVLSLDLEDHINIEMAGATGFLEALAITAYHQQLTATPLLVNDDLLSEINKLPHPQR
                     TNPFIDRHGNLERAKTILAGLFMVLTNADKWQLFI"
     gene            complement(1663..1914)
                     /locus_tag="PWX02_002214"
     CDS             complement(1663..1914)
                     /locus_tag="PWX02_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730184.1"
                     /translation="MRLAIRHYLDNDKGNQTPLFTFLSLYSETEPYPLPELLIVLGNR
                     IAKLEQQHNAMPSETDSITLGILRKQLSQLLKVAERIKE"
     gene            complement(2007..2618)
                     /locus_tag="PWX02_002215"
     CDS             complement(2007..2618)
                     /locus_tag="PWX02_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HDL1730185.1"
                     /translation="MMKEINQAVFPKVFHKETVAMTNTLKVAEYFEKRHDNILKRLET
                     LECSAEFSLLNFKAAKYIDEQGKSRKMYLMTQDGFTLLVMGFTGKKAIQFKESYITAF
                     NAMRKQIIARAELKSDQLRLNEAIKLNLEKTGKQDIHAYARENNLVYITALGATAKKW
                     LLAQGYSANDEIRHHLNEQQLKLVDDLLAENAVMIKLGLEYDQ"
     gene            complement(2626..2859)
                     /locus_tag="PWX02_002216"
     CDS             complement(2626..2859)
                     /locus_tag="PWX02_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HDL1730186.1"
                     /translation="MTESTITHLADDVRIIRPIHARQKLGVGKTTFQDWQNPKSKRYR
                     PDFPKKIQLGANSVGYLESEINAFIQSLADNRV"
     gene            complement(3048..>3712)
                     /locus_tag="PWX02_002217"
     CDS             complement(3048..>3712)
                     /locus_tag="PWX02_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730187.1"
                     /translation="EVEVLYAPYGEFQISELRYSYASVYFPVFDGLGKQFDIINGMVE
                     SRMLESLSERNFLNSKLFYKMLQKSLNNYYVVNSPFDQFYFKLKEIIFYPIGLRPYCV
                     SSEELDNNNDLKEIYDEEDFSNIFYFQKEELEAYIDKNLKIETKLEQTDITSIFDGDI
                     PPKTDQDKLAHFIELIIQKSEFMKDGKIPTYSELHTMLSHKYPHDAIPSKNTIKKYLG
                     QH"
BASE COUNT          931 a          719 c          767 g         1295 t
ORIGIN      
        1 cgcaaaagaa tagttgcttg tgggttcggt gtgcttttcc cttgttccca cccttgatag
       61 gttttaacgc tggttcgtaa acgcttggca aataccgctt gagagagatt tagctttgct
      121 ctgattgctt ttacttcatc ggctgaaatc tgtagcggtt cagggcgtga tagggtttca
      181 gttcgcagtg ttcgcttgcc ctctaaatgc tctttcattg cggttaggct ttccattaaa
      241 tcattataaa aactcatatc taccccttag agagcagttt caataaaagt taaattcatc
      301 ttatctattc cgttgcggtt ctactgggtg ttatagtatc cgcaaagtga ggataaatca
      361 ataaaacaag taacaaaaaa gcctagcata tttcatctag gctttgcttg ttggcggtag
      421 gggttagcct tgccacttgt taaaggtgcg tagtttgtcg atatagtgaa catttgtaac
      481 cgtccgcccg ttgctttccc ttgtttggta ttcccctttg tctaattcct taagggcttg
      541 tttaaacgct cgggtaaagg ttcggcggtt gattggctct atatcgttga gctggcaaaa
      601 gtagatataa gctggataaa tcgcactgtc tcttgtgccg ttattaagtg aacttcccat
      661 tgccagcccg tttactgtcg gcaatatttc aaactcttga gcaaactcta aaacgtggtt
      721 cacttccatt ttaaccgcta gagcttcttg gctcactcgt tgcttttcta gtagggcttt
      781 tgcttcgagt ggttcgggga aagtgtcaaa cagtaggcga ataatgccgc ccgtttcggc
      841 ttgaatttta tcaattaaat taaagtccct ttcttcaatc ggcacaatgc gatcaaactt
      901 aaaaatcact ctccgccttt ccgtgcctcc gctgttttcg gtaaacagaa tcggggtgtt
      961 attggttgtc ataaagaccg cattcacttt ttggctaaac ggctttttgt gttttgggtt
     1021 gaagaatata tcatcgcccc ctgtgattgc cctaatcgtt gccccgtccc cggtatattt
     1081 cggttgctct ccggagatga taaatgtttt atcaatgata atgcaacgct taacttcatc
     1141 atcaagagct tttagcgtta tgcctacgtg gttattttca cctcccagca gtttcgctat
     1201 ttcggtgtaa gtgcttttcc ctgttccgcc tagccctgtt acctagataa ataattgcca
     1261 tttgtcggcg ttagttagca ccataaacaa gccggctaaa atcgtcttag ctcgctctaa
     1321 gttgccgtgt cggtcaataa atgggtttgt tcgttgtggg tggggtagct tgttgatttc
     1381 actcaataag tcatcattca ctaatagcgg cgtggcggtt agctgttgat gataggcagt
     1441 aatcgctaga gcctctaaaa agcccgtagc ccctgccatt tcaatattga tatgatcttc
     1501 aaggtcaaga gaaagcacac cgataaaaaa cggtctgccg ttatcgtggt acagtcgttc
     1561 aattcggata tgcggaaaat ctaaaaagct caaggttgct ttgataggtt cactcattaa
     1621 agcagtaata cccgctaaat taagctcgtg tttcattttg gtttattcct ttattctttc
     1681 cgccactttt aacagttggc taagttgttt tcttaaaatg ccgagtgtga ttgaatcggt
     1741 ttcgcttggc attgcgttat gttgttgttc cagcttcgct attcggttgc ctagcacaat
     1801 taaaagctcg ggcaatgggt aaggctcggt ttcagaatag aggcttaaaa acgtgaataa
     1861 gggtgtttga ttgcctttgt cgttatcgag atagtgccga atagccagcc gcattaaaac
     1921 ggggttttgc ttggcggtaa tgcacctcat tatttaccct cttgtttgcg gtagtaactt
     1981 aatgcggttt cttgtagcct tgcttttcat tggtcgtatt ccagccctaa cttaatcatc
     2041 acggcatttt ctgccagcaa gtcatcaacc agtttgagct gttgttcgtt taaatggtgt
     2101 cggatttcat cattcgctga ataaccttga gcaagtagcc attttttagc agttgctcct
     2161 agtgccgtga tgtaaacaag gttattttct cgggcgtagg cgtgaatatc ctgcttgcct
     2221 gttttctcaa gattgagttt gattgcctcg tttagtcgga gctgatcgct tttcaattca
     2281 gcccttgcga taatttgctt tctcattgca ttaaacgctg tgatatagct ttctttgaat
     2341 tgtattgctt tcttaccggt aaagcccatc actaaaagcg taaagccgtc ttgggtcatt
     2401 aaatacatct ttcttgattt gccttgctca tcgatatatt tagccgcctt aaaattgagg
     2461 aggctaaatt cagcagaaca ttctaaggtt tcaaggcgtt tcaaaatgtt gtcgtgtcgt
     2521 ttctcgaaat attccgccac tttgagcgtg ttagtcattg ctacggtttc tttgtgaaag
     2581 actttcggga atacggcttg attgatttct ttcatcattt cccccttata ctcggttatc
     2641 tgccagcgat tgaataaagg cgttgatttc gctttcgaga taacccacgg aatttgcccc
     2701 tagctgaatc ttcttcggga agtctgggcg atagcgtttt gatttcgggt tctgccagtc
     2761 ttgaaaagtg gttttgccta cgcctaactt ttggcgtgcg tgaatagggc ggataatgcg
     2821 aacatcatca gctaagtgtg tgatagtgct ttcggtcatt tatattcccc tatgctgtta
     2881 aattgaaatg cttaaacctt gaaagggttt aggcggttgc agtagggatt ttaagaaatg
     2941 aaaagttatt caggggaaga gtgaactatt gaacagtgtt cattgaacaa atgaactgtt
     3001 gttgaacggt ttaaataaaa aaatccgcca taattagcgg attttgatta gtgttgtcct
     3061 aaatatttct tgattgtatt tttagatggg attgcatcgt gtggatattt atgacttagc
     3121 attgtatgaa gttcactata ggttggtatt ttaccgtctt tcataaactc acttttttga
     3181 ataattagct cgataaaatg agccaattta tcttgatctg tttttggtgg gatatctcca
     3241 tcaaatattg atgttatgtc tgtttgttct agttttgttt ctatttttag gtttttatca
     3301 atgtaggctt ctagttcttc tttttgaaaa tagaatatat ttgaaaaatc ctcttcatcg
     3361 taaatttctt ttaaatcatt attattatct agttcctcag atgatacgca ataaggccgt
     3421 aagccaatag ggtaaaatat gatttctttt aacttgaaat aaaactgatc aaatggactg
     3481 tttaccacat aataattatt taaacttttt tggagcattt tataaaatag ttttgaattt
     3541 aaaaaatttc tctcggatag agattctaac attcggcttt caaccatacc attaataata
     3601 tcaaattgct ttcctaaccc gtcgaatact ggaaaataaa cagacgcata agaataacgt
     3661 aattcactaa tctgaaactc gccataagga gcatataaaa cttcgacttc at
//
LOCUS       DANJWC010000190         3633 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.190, whole genome shotgun sequence.
ACCESSION   DANJWC010000190 DANJWC010000000
VERSION     DANJWC010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.190"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            88..669
                     /locus_tag="PWX02_002218"
     CDS             88..669
                     /locus_tag="PWX02_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL1730188.1"
                     /translation="MSQPALRQISPSQLEDIRLQMVKFAFLQLKNNELAEDVVQEAFV
                     SAYKYADSFKGESALKTWIFAILKNKIVDLVKAKGKLVAVSELMEEDDHDLADKLFNE
                     NGGWDKDAYQSNEWKNTESHTYSAQFWQVFEFCLNNLPANQARVFMMRSHLEMETEEI
                     CAECEISPANVHTLLYRARLQLQVCLSQKWFGG"
     gene            670..840
                     /locus_tag="PWX02_002219"
     CDS             670..840
                     /locus_tag="PWX02_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HDL1730189.1"
                     /translation="MLKCKQITELVSLSQEQKLTLKQRVNVQVHLMLCSRCRAFSQNC
                     KTVDKLTKEFNS"
     gene            complement(889..2106)
                     /locus_tag="PWX02_002220"
     CDS             complement(889..2106)
                     /locus_tag="PWX02_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family transporter"
                     /protein_id="HDL1730190.1"
                     /translation="MGASLIGALTITQAIIAMSFGCIVIAIGLVLIGNGGHKYGIPFP
                     IQLRSSFGTTGVKIPGILRGVPAIVWFGFQSWVGAGAINMCLKLLFGFDNLPVVYVLF
                     TVLQVGLSINGFKGIKWLENVSCVFLIGILAYMLYVVNTKFSVEIGERFANIEGTWGM
                     PFWAVTTAFLGIYSTMILNASDYSRHVKKGTTPTATGVIYVLSILPVTLFMGLIGLLV
                     TAATGNSDPIEVFATAMDNKFLTVITLLFIAFAQVTTNVLNNIVPPSYILMDSFKMKW
                     SHATILVGILSICVMPWKLVTAESADGLSLFIKIYSAFLGPIFAVMVVDYYILRKRTL
                     NVNDLYNKEGIFKGINWAAIIAITIGSLCSLIVVDLSWYVSLLPSGLSYYFLMKVLKS
                     SASFRKGTVLEHQ"
     gene            complement(2091..2246)
                     /locus_tag="PWX02_002221"
     CDS             complement(2091..2246)
                     /locus_tag="PWX02_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730191.1"
                     /translation="MQHNTQLNPELAKEVNVDLQPTKERIMDNFSYWASFLVAVFLLV
                     HFLWGQA"
     gene            complement(2249..3628)
                     /gene="allB"
                     /locus_tag="PWX02_002222"
     CDS             complement(2249..3628)
                     /gene="allB"
                     /locus_tag="PWX02_002222"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HDL1730192.1"
                     /translation="MYDLIIKNGKIVLSDTVFNGNIAVNSGKIAAILEEGTEPDAKDV
                     IDVKGNYVFPGAIDTHTHLNDPGYEWREDYAHGTAAAVGGYTTIIDMPVQNEPALTNG
                     KIFDAKMAKVSPNAYVDYCFWGGLVPSNFEHLSELNDKGCVALKSFIGPVSPDYASLN
                     YGQTVEAMEIIQKFGGRAGFHCEDFSTIKWQEQRMKNEGRLDWRAFLDSRPFIAEMLA
                     TINIIEIAKATGCKAHICHVSHPDVAQKIKDAQEEGYDITAETCAHYLCFSEDDVIEK
                     GALFKCAPPLRSKSDVDRLWNYVVDGTFSGISSDHSPCSYDEKYNEILGQKINNVFDV
                     WGGISGIQSCVQVAFYEGCIKRGISPTVLANVMAKLPAKAFGIYGQKGDIQVGFDADF
                     TIIDPNKEWEIKAEDLQYVNKISAYVGKKGQGLPVCTIVRGKVIAQDGKLTDSKGFGK
                     FITKTKQGA"
BASE COUNT         1185 a          752 c          641 g         1055 t
ORIGIN      
        1 taccaatatc ccctaagttg ggtaaaacct tatgaaatag gatacaagcg gttagtttct
       61 ctcaattttt taccaaagga tcacgcaatg agccaaccgg ctttacggca aatatccccc
      121 tcgcagctag aagatattcg tctgcaaatg gtgaagtttg cctttttaca gttaaaaaat
      181 aacgaacttg ccgaagatgt ggtgcaagag gcgtttgtca gcgcttataa atatgccgac
      241 agcttcaaag gtgaaagtgc gttaaaaacg tggatttttg caattttaaa aaacaagatc
      301 gtagatttag tgaaagccaa aggcaaattg gtggcagtat ctgagctgat ggaagaagac
      361 gatcacgatc ttgccgataa attgtttaac gaaaatggcg gttgggataa agatgcttat
      421 caatctaatg agtggaagaa caccgaaagc catacctatt ccgctcaatt ctggcaagta
      481 tttgaatttt gcctaaataa tctgccggca aatcaagcaa gggtgtttat gatgcgttcc
      541 catttggaaa tggaaaccga agaaatttgc gcagaatgtg agatttcacc agcaaatgtg
      601 cataccttac tgtatcgggc aagattgcag ttacaggtct gtttaagtca aaaatggttt
      661 ggaggttaaa tgttaaaatg taagcaaatt accgaattag tttctctgtc ccaagaacaa
      721 aaactgacat taaaacaacg tgttaatgtg caagttcatt taatgctttg ttctcgttgc
      781 agagccttta gccaaaactg caaaaccgta gataagctaa cgaaagaatt taactcttag
      841 cgtaaaagca agcggtaaaa tttttaataa attttaccgc ttgtcatctt attgatgttc
      901 tagcactgtc cctttacgga aagatgctga acttttcaat actttcataa gaaagtagta
      961 gcttaaacca gaaggaagta aactcacata ccaagaaaga tcaaccacaa tcaatgagca
     1021 aagtgaacca attgtaatag ctatgattgc agcccagtta atccctttga aaataccttc
     1081 tttattatat aagtcattta cgtttaaagt acgttttctt aagatgtagt aatcgacgac
     1141 cattaccgca aaaatagggc ctaagaatgc cgagtaaatt ttaataaaca aagataatcc
     1201 atctgctgat tcagcagtta ccaacttcca aggcattaca catatagaaa gaatgcccac
     1261 taaaatggtg gcatgcgacc atttcatttt aaatgaatcc attaaaatgt aagatggcgg
     1321 aacaatatta ttcaacacgt ttgtcgtaac ctgtgcaaaa gcaatgaata ataacgttat
     1381 tactgttaaa aatttattat ccattgcagt tgcaaatact tcaattgggt cactattgcc
     1441 tgtagctgcc gtaactaaca accctattaa acccataaat aaggttacag ggaggattga
     1501 taatacataa atcacacctg ttgctgttgg ggttgttcct tttttaacgt ggcgagaata
     1561 atcggaagca tttaaaatca ttgttgaata aatacccaag aatgctgtag ttacagccca
     1621 gaatggcatt ccccaagtgc cttcaatatt ggcaaagcgc tcgcctattt ctacagagaa
     1681 ttttgtattc acaacataaa gcatataagc aagaataccg atgaggaaca cacaagaaac
     1741 attttccaac catttaatac ctttaaaacc attaatggat aagccaactt gaagaacagt
     1801 gaatagcaca taaacaacag gcaagttatc aaaaccgaac aacagtttta aacacatatt
     1861 gatagcacct gcccccaccc aactttggaa tccaaaccaa acgattgcag ggacaccacg
     1921 taaaatacca ggaattttta cacctgttgt accaaaagag cttcgtagtt ggattgggaa
     1981 tggaatacca tatttatgcc cgccattgcc aattagcaca aggccgatag caatcacaat
     2041 acaaccaaac gacattgcaa taatcgcttg tgttatcgtt aacgctccaa tcaggcttgc
     2101 ccccatagaa aatgtaccaa tagaaacaca gccaccaaaa aacttgccca ataggaaaag
     2161 ttatccataa tgcgttcttt ggttggctgt aagtcaacat ttacttcttt tgctaactca
     2221 gggttaagtt gtgtgttgtg ttgcattgtt atgctccttg ttttgtttta gtaataaatt
     2281 ttccaaaacc tttgctgtcg gtgagtttcc catcttgagc aatcacttta ccacgtacga
     2341 tggtgcaaac tggtaaacct tgaccttttt tgcccacata agcggagatt ttattcacat
     2401 attgcagatc ttccgctttg atttcccact ccttgtttgg gtcaataatc gtaaaatctg
     2461 catcaaatcc aacttgaata tcgcctttct gaccataaat accgaacgct ttagctggta
     2521 attttgccat tacatttgcc aatacagttg gagatattcc acgctttata caaccctcat
     2581 agaaagccac ttgtacacaa ctttgaatgc cactaatccc gccccataca tcaaatacat
     2641 tgttaatttt ttgacctaaa atttcattgt acttttcatc ataagaacaa ggagaatggt
     2701 cgctactaat gccgctaaat gtcccatcta ccacataatt ccatagacga tcaacatcag
     2761 attttgagcg aagtggcgga gcacatttga acaacgcccc tttttcaatc acatcatctt
     2821 cactaaagca gagataatgt gcacaggttt cagcggtaat atcatagccc tcttcttgag
     2881 cgtctttaat tttttgagca acgtctgggt gactaacgtg gcaaatatgc gccttacagc
     2941 ccgtcgcttt agcaatttca ataatattaa tggtggcaag catttctgca ataaacggac
     3001 gggaatctaa aaatgctcgc caatctaaac gcccttcgtt tttcattctt tgctcttgcc
     3061 atttgatggt ggaaaaatct tcgcaatgaa aacctgcacg accaccaaat ttttggataa
     3121 tttccattgc ctcaacagtt tgaccatagt taagtgaagc ataatcagga gaaacaggcc
     3181 caataaagga ctttaacgcg acacagcctt tatcatttaa ttcacttaaa tgctcaaaat
     3241 tacttggtac taaaccaccc cagaaacagt aatcgacata cgcatttggc gatactttag
     3301 ccattttggc atcaaatatt ttgccattag ttaaagcagg ctcattttgc acaggcatat
     3361 caataatggt ggtatatccg ccaactgcgg cggctgtacc gtgtgcataa tcttcacgcc
     3421 attcataacc tgggtcatta agatgggtgt gagtatctat tgcccctgga aaaacataat
     3481 ttcctttaac atcaataacg tcttttgcat ctggctctgt gccttcttct aaaatagcag
     3541 caatcttacc tgaattaacg gcaatattac cgttgaaaac ggtatcggat aagactatct
     3601 tcccattttt tatgatgaga tcatacatag tca
//
LOCUS       DANJWC010000191         3597 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.191, whole genome shotgun sequence.
ACCESSION   DANJWC010000191 DANJWC010000000
VERSION     DANJWC010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.191"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(16..138)
                     /locus_tag="PWX02_002223"
     CDS             complement(16..138)
                     /locus_tag="PWX02_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HDL1730193.1"
                     /translation="MKKLVLTILAAATFLTACNTVDGVGKDVEKAGDKIQQAAQ"
     gene            complement(218..1195)
                     /gene="rluD"
                     /locus_tag="PWX02_002224"
     CDS             complement(218..1195)
                     /gene="rluD"
                     /locus_tag="PWX02_002224"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HDL1730194.1"
                     /translation="MTQQKILTAEITADLLGARLDQALAQLFPDYSRSRIKVWIESDL
                     VKVNGNIVNKAREKVFGGETVEITAEIEEEIRFEPQDIPLNIVYEDDDIIVINKPKDL
                     VVHPGAGNPDGTVLNALLHYYPPIAEVPRAGIVHRLDKDTTGLMVVAKNIPAQTHLVT
                     ALQKRRITREYEAIASGIMTQGGKVDEPMARHPTKRTAMAVHPMGKPAVTHYRIMERF
                     RNYTRLRLRLETGRTHQIRVHMAHIAHPLLGDQLYGGRPRPPKGASDSFLSVLRGFQR
                     QALHATMLRLEHPITGEMMEWHAPLPDDFIELIQALKADYELYKDQLDY"
     gene            1336..2115
                     /locus_tag="PWX02_002225"
     CDS             1336..2115
                     /locus_tag="PWX02_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HDL1730195.1"
                     /translation="MRKFKSLATLVLAGLFVAGCSNSNKELEQSNVQDLYGKGQTYLQ
                     DGDYNSAIRYLEAVGTKGGQYSGLGEQTQLSLIYAQYKVGEYYKALDAAERFARSYPN
                     AANMDYVFYLAGLSNARLSDNFIQDFFGVNRASRAVDNVRNAYGNFQTIVQRYPQSQY
                     AQDAQNWMNYLFNRLAEHELAVVKFYDERNAYVAVVNRIEEMLRFYPNTLATKQALPY
                     LKEAYKQMNIPDAEQKTELLIQEYESKELPNPEKPKYGEQF"
     gene            2188..3219
                     /gene="tsaD"
                     /locus_tag="PWX02_002226"
     CDS             2188..3219
                     /gene="tsaD"
                     /locus_tag="PWX02_002226"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDL1730196.1"
                     /translation="MRILGIETSCDETGVAIYDEDKGLVANQLYSQIDMHADYGGVVP
                     ELASRDHIRKTLPLIQEALKEANLQPSDIDGIAYTAGPGLVGALLVGSTIARSLAYAW
                     NVPALGVHHMEGHLLAPMLEENAPEFPFVALLISGGHTQLVKVDGVGQYELLGESIDD
                     AAGEAFDKTGKLLGLDYPAGVAMSKLAESGTPNRFKFPRPMTDRPGLDFSFSGLKTFA
                     ANTIKANLNENGELDEQTKCDIAHAFQQAVVDTILIKCKRALEQTGYKRLVMAGGVSA
                     NKQLRADLAEMMKKLKGEVFYPRPQFCTDNGAMIAYTGFLRLKNDEQTDLSISVKPRW
                     AMTELPPIN"
     gene            3301..>3597
                     /locus_tag="PWX02_002227"
     CDS             3301..>3597
                     /locus_tag="PWX02_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HDL1730197.1"
                     /translation="MNNYVISLTSAQERRKHIEAEFGKQNIPFQFFDAITPDLIKEKA
                     KAFNIDISNTNLTKGEIACALSHIALWHLAKQQNLDYICIFEDDIYLGNNAFELL"
BASE COUNT         1090 a          751 c          696 g         1060 t
ORIGIN      
        1 attcgaagct ttttgttatt gtgcagcctg ctgaatttta tcgcccgctt tttcaacatc
       61 cttaccaaca ccatcaacgg tattacacgc agtcaagaaa gttgcagctg ctaaaatagt
      121 taatactaat tttttcatag aaagctcctt ttttgttgtt tacttatgaa aacgatttca
      181 atatatcaac tttacattat ttaagcaaca cctaaactta ataatctaat tgatctttat
      241 aaagctcata atccgctttt agtgcctgaa tcaattcaat aaaatcatca ggtaacgggg
      301 cgtgccactc catcatttca ccagtaatcg gatgctctaa tctcaacatt gtggcgtgta
      361 aggcttggcg ttggaagcct cgtaacactg ataaaaacga gtcactcgca cctttaggtg
      421 ggcgaggtct accgccatag agctgatcac ctaataatgg gtgagcaata tgcgccatat
      481 gtacccgaat ttggtgagta cgccctgttt ctaatcttaa acgtaaacgt gtgtaattac
      541 gaaaacgctc cataatacga taatgcgtaa ccgccggttt tcccattggg tgaacagcca
      601 ttgccgtacg tttagttggg tggcgagcca ttggctcatc aacttttccg ccctgtgtca
      661 taatgccgct ggcaatcgct tcatattcac gggtaatacg acgtttttgt aatgcggtta
      721 ctaagtgagt ctgtgccgga atgtttttag ccactaccat taagcctgtc gtgtctttat
      781 ccaatcggtg aacaatccct gctcgaggca cttctgcaat cggtggataa taatgtagca
      841 aagcattcag cactgtaccg tccggattac cagcccctgg gtgaaccact aaatccttcg
      901 gtttattaat gacgataata tcgtcatctt catacacaat atttagagga atatcttgag
      961 gctcaaaacg aatttcctct tctatttcag cggtaatttc aacagtttca ccaccaaata
     1021 ctttttccct tgctttattc acgatattgc cattaacttt cactagatca ctttcaatcc
     1081 acactttaat gcgtgaacga gaatagtccg gaaaaagttg tgccagagcc tgatctaggc
     1141 gagcacctag taaatctgca gtaatttctg ctgttaaaat cttttgttga gtcattaaac
     1201 cataaatcct aattagtcat ttgcttactt gcgtgatgtt atatagaata gcgagatgaa
     1261 cttcataagt tattcttagt cagaggacat tgtacattaa cttttcgtcc tagacacttt
     1321 ttataggtaa aacatatgcg taaatttaaa tctcttgcca cattagtatt agccggactg
     1381 tttgttgcag gttgttcaaa ttcaaataaa gaattagaac aatccaacgt tcaagatctt
     1441 tatggcaaag gtcagaccta tttacaagat ggagattata attctgccat tcgctactta
     1501 gaagcggttg gaaccaaagg aggccaatat tcaggacttg gtgagcaaac ccaactaagt
     1561 ttaatttatg ctcaatataa agttggcgaa tactataaag cacttgatgc cgcagaacgt
     1621 tttgctcgct cttacccgaa tgcagctaat atggattacg tattctatct agcaggatta
     1681 tcaaatgcac gtctaagtga taattttatt caagacttct ttggtgtaaa tcgcgcctct
     1741 cgagccgtag ataatgttcg taatgcttac ggtaacttcc aaactattgt acagcgctac
     1801 cctcaaagcc aatatgcaca agatgctcaa aattggatga attacttatt taaccgttta
     1861 gcggaacacg aattagccgt tgttaaattt tatgatgaac gtaatgccta tgtggctgta
     1921 gtaaaccgta tcgaagaaat gttgcgtttt tacccgaata cactggcaac caaacaagca
     1981 ttgccatact taaaagaagc atataagcaa atgaatatcc cagatgcaga gcaaaaaacg
     2041 gaactcttga tccaagaata tgaaagcaaa gagctaccga atcctgaaaa accgaagtat
     2101 ggcgaacaat tctagtcgta cagagaataa tgtgaggggc gttcttcgcc ccttttggtt
     2161 ttctaactta ttttgacttc tccaactatg cgaattttag gtattgaaac ctcttgtgat
     2221 gaaaccggtg ttgccattta tgatgaagac aaaggcttag tggcaaacca gctttatagc
     2281 caaattgata tgcacgccga ttacggtggc gtagtccctg aactggcttc tcgagaccat
     2341 atccgtaaaa cgttgccact aattcaagaa gccttaaaag aggccaatct gcaaccctcg
     2401 gatattgacg gcattgccta tactgccggc ccaggcttgg tcggggcttt attggtcggc
     2461 tcaaccattg cccgttcgct ggcttatgct tggaatgttc cggcattggg cgttcaccat
     2521 atggaagggc atttacttgc cccaatgttg gaagaaaatg cccctgaatt tccgtttgtg
     2581 gcattattga tttcaggtgg acacacccaa ctggtaaaag ttgacggcgt tgggcaatac
     2641 gaactactcg gggaatcaat tgatgatgct gccggtgaag cctttgacaa aacaggcaaa
     2701 ctactcggtt tggattaccc tgccggtgta gcgatgtcaa aattagccga atccggcacg
     2761 ccaaatcgtt ttaaattccc tcgtccaatg accgacagac cgggactgga tttcagtttc
     2821 tccggtttaa aaacctttgc tgcgaatacg attaaagcca atcttaatga aaatggtgaa
     2881 ctcgatgagc aaaccaaatg cgatattgcc cacgcattcc aacaagccgt ggttgatact
     2941 attttaatta aatgcaagcg agcgttagag caaaccggct ataaacgctt agtaatggca
     3001 ggcggcgtaa gtgccaataa acaattacga gcagaccttg cggaaatgat gaaaaaatta
     3061 aaaggcgaag tattctaccc tcgcccacaa ttttgcactg acaacggcgc aatgattgcc
     3121 tacactggct ttcttcgctt aaaaaacgat gaacaaaccg acttaagcat tagcgtaaaa
     3181 ccccgctggg ctatgaccga attaccaccg attaattaac ctttcaagcg gtgaaatttc
     3241 ttgttaattt tgcaaaaatt taatcaaaaa taaccgcttg ctatatgata gattaaattt
     3301 atgaataatt atgtaattag cctaacctcc gcacaggagc gtagaaaaca tattgaagct
     3361 gaattcggca aacaaaatat tccttttcaa tttttcgatg cgattacgcc ggatttaatt
     3421 aaagaaaaag ccaaagcatt taatattgac atttcaaata caaacttaac aaaaggtgaa
     3481 atagcctgtg ctttgagtca tattgcatta tggcatttag ccaaacaaca gaatttggat
     3541 tatatttgta tttttgaaga tgatatttat ttaggtaata atgcatttga attacta
//
LOCUS       DANJWC010000192         3499 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.192, whole genome shotgun sequence.
ACCESSION   DANJWC010000192 DANJWC010000000
VERSION     DANJWC010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.192"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..513)
                     /locus_tag="PWX02_002228"
     CDS             complement(<1..513)
                     /locus_tag="PWX02_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1730198.1"
                     /translation="MIITMKDIAKEANVSLGTVSNVLNGKSNVSLAKIEKVQAAVEKL
                     GYRKNIQASTLKSGTSNKIAIILPNITELKYACLYENLDLICTQNDLSLILYLTHNRE
                     DREKQIIQNIKQDNYLRIILDSCLNNADYYYDEVKDQDKFIFVYRYLKNAKHFIGFDY
                     LPVIGDLIKHM"
     gene            complement(675..2015)
                     /gene="gdhA"
                     /locus_tag="PWX02_002229"
     CDS             complement(675..2015)
                     /gene="gdhA"
                     /locus_tag="PWX02_002229"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDL1730199.1"
                     /translation="MSDLNALFQKIKQRDPNQAPFHQAVEEVFGSLAPFLAKNPKYTQ
                     QGLLGRIVEPERVISFRVTWVDDKGQVQVNRGYRVQMNSAIGPYKGGIRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMSELYRHIGADTD
                     VPAGDIGVGGREIGYMFGQYKKLRNEFTSVLTGKSLTWGGSLIRPEATGYGAVYFAES
                     MLATKGQQIEGKSVVISGSGNVAQYAAEKVIQKGGKVLTVSDSNGYVLFPESGMTEAQ
                     LQALLELKNERRERLSVYATEQGLKYFDGQKPWGVKCDIALPCATQNELDTEDAKELV
                     KNGCICVAEGANMPTTLGGVEVFIGAKILYAPGKAANAGGVATSGLEMSQNAIRLSWS
                     REEVDQRLFNIMKAIHENCVENGTEADGFVNYVNGANIAGFKKVANAMLEQGIV"
     gene            complement(2208..2549)
                     /gene="bamE"
                     /locus_tag="PWX02_002230"
     CDS             complement(2208..2549)
                     /gene="bamE"
                     /locus_tag="PWX02_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HDL1730200.1"
                     /translation="MKMKSLLAAMLLALGVTACSTVEKVVYRIDVPQGNYLEQDKIAQ
                     LKVGMTAEQVEYLLGTPMLKNIFETHRWDYVFIKREGYKDPEQRNLFIYFDAKGLVSK
                     IDDSNANAEQK"
     gene            complement(2616..>3499)
                     /gene="yejK"
                     /locus_tag="PWX02_002231"
     CDS             complement(2616..>3499)
                     /gene="yejK"
                     /locus_tag="PWX02_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HDL1730201.1"
                     /translation="MMLQLHQAYQSKAKAYGIFKPESVFAQQLNRLLEQETDFLPFSH
                     SCAKMLAAELIKYPFADGGTFILCRYTFLATEYLFIALIDSRNSMLVDEQLEIKRTQY
                     LEIAQYDIACRINLTELKLDATSNRYLTFVKGRVGRKIADFFMDFLSAEEGFNPQLQN
                     QTLLQAVSDYCEQGELSAPQTREVKKQVFEYCKGQINSGEEIALNELSNALPTLNEID
                     FAQFTQEQEYGLEENIPPMRNALKTLTKFSGSGKGVTISFNAELLGERLIWDELNDTL
                     TIKGLPANLRDQLERNK"
BASE COUNT         1048 a          742 c          578 g         1131 t
ORIGIN      
        1 catatgcttt ataagatcac caatcacagg aagataatca aaaccaataa aatgttttgc
       61 atttttaagg taacgataaa caaaaataaa tttatcttgg tcttttactt catcgtaata
      121 atagtctgca ttatttaggc aactatctaa aataatacgc aaatagttat cttgctttat
      181 attttgaata atttgttttt ctctatcttc tcgattatgc gttaaataaa gaatgagaga
      241 taaatcattt tgcgtacaaa ttaaatcaag attttcatat aaacaggcat atttcagctc
      301 tgtaatattg ggcaatataa tagcaatttt attacttgtg ccacttttta acgtagaggc
      361 ttgaatattt ttacgataac ccaacttttc aacggcagct tgtacttttt ctattttagc
      421 taagctaaca ttactttttc cgtttaatac attcgatact gttcctaacg aaacatttgc
      481 ttcctttgcg atatctttca tcgtaatgat catatactgc cctttcacat actgctaaaa
      541 ctgagattgg catagatttt accataattg gcaaatgaaa tacgatttca atttagcttg
      601 tctctagcta caacctaaaa taaaaaaact gcctcgatat atcgaggcag ttcttgaata
      661 atagggataa taaattaaac aataccctgt tctaacatcg catttgccac ttttttgaaa
      721 ccagcaatat tcgcaccgtt tacatagttt acgaaaccat ctgcttcagt accattttct
      781 acgcagtttt cgtgaattgc tttcataatg ttgaataaac gctgatctac ttcttcacga
      841 ctccaagata gacgaattgc attttggctc atttctaaac ctgaggttgc cacaccacct
      901 gcgttagccg ctttacccgg agcataaagg attttcgcac cgataaagac ttcaacacca
      961 cctaatgtgg ttggcatatt tgcaccttct gccacacaaa tacagccgtt tttcactaat
     1021 tctttagcat cttcggtatc taattcgttt tgggttgcac aaggtaaagc gatgtcacat
     1081 ttcacacccc acggtttttg accgtcaaag tattttaagc cttgttctgt tgcatacaca
     1141 gataagcgtt cacggcgttc gtttttcaac tctaataatg cttgtaattg tgcctctgtc
     1201 ataccggatt cagggaataa gacataaccg ttagaatctg aaaccgttaa tactttaccg
     1261 cctttttgaa tcactttttc agctgcatat tgtgccacgt tacccgaacc ggaaattacc
     1321 actgattttc cttcgatctg ctgaccttta gtcgctaaca tagactctgc aaaatacacc
     1381 gcaccataac cagttgcttc cggacgaatt aagctaccac cccaagtcag cgatttaccg
     1441 gttaatactg aagtaaattc attgcgtaat tttttgtatt gaccgaacat atagccgatt
     1501 tcacgacctc ccacgccgat atcgcctgca ggaacgtcag tatcagcacc aatgtggcga
     1561 tataattcgc tcataaacgc ttggcagaaa cgcattactt cggcatcaga tttgcctttc
     1621 gggtcaaagt ctgaaccacc tttaccaccg cccattggca atgtcgttaa cgcatttttg
     1681 aatacttgct caaaggctaa gaattttaat acgcctaaat caacggttgg gtggaaacga
     1741 ataccgcctt tgtatggacc gattgctgag ttcatttgta cacggtaacc acggtttact
     1801 tgcacttgac ctttatcatc aacccaagtg acacggaagc taattacacg ctcaggctca
     1861 acaatacgcc ctaataaacc ttgctgggta tatttcggat ttttagctaa gaaaggggca
     1921 aggctaccga acacttcttc aaccgcttga tggaaagggg cttggttcgg atcacgttgt
     1981 ttgatttttt ggaaaagggc gtttaaatcg ctcatattat actcctgaat aaattgatga
     2041 tgttgtgtat ttaagatgag cgaagtctag caagcaattt tagctttgaa aagatagatt
     2101 agaaaaattt atcattaaaa taagaaaaat taagtttaaa tgcttatttt ttattcaaaa
     2161 aaataagccc acttttaaaa gtgggctttt cgaaccagca aatcgtccta tttttgttct
     2221 gcattcgcat tgctgtcatc aatttttgat actaaccctt tagcatcaaa ataaataaac
     2281 agattgcgtt gttcaggatc tttataacct tcacgtttaa taaacacata atcccaacgg
     2341 tgggtttcaa aaatgttctt taacattggt gtaccaagca aatattcaac ttgttccgct
     2401 gtcataccga ctttaagttg agcaatttta tcttgttcta agtaattgcc ttgcggtaca
     2461 tcaatacggt acaccacttt ctcaacggtt gaacaagccg ttacacccag tgcaagcaac
     2521 attgcagcta aaagagattt cattttcata attgaccttt tgataatcca aaattcagat
     2581 aacgagataa tacctaaact gataaaaatt ttccattatt tgttacgttc cagctggtca
     2641 cgcagatttg ccggtaatcc tttgattgtc agtgtgtcgt ttagttcatc ccaaattaaa
     2701 cgctctccta ataattctgc gttaaagcta atcgttactc ctttgcccga accggaaaat
     2761 ttcgtcaggg ttttaagggc atttcgcata ggcggaatat tctcttccaa gccgtattct
     2821 tgctcttgcg taaattgggc aaaatcgatt tcattcagtg ttggcaatgc gttggaaagc
     2881 tcatttaacg caatttcctc tccactattt atctgccctt tacaatattc aaacacctgc
     2941 tttttcactt ctcgagtttg aggggcagat aactcccctt gctcacaata atcgctcacc
     3001 gcttgcaaca aggtttgatt ttgcaattgt ggattaaacc cttcttccgc acttaaaaaa
     3061 tccataaaga aatcggcaat tttacgccct actctgcctt tcacaaaagt taaatagcga
     3121 tttgacgttg cgtcaagttt cagttcagtt aaattaatgc ggcaggcaat atcatattgt
     3181 gcaatttcga gatattgcgt gcgtttaatc tctaactgct catcaaccag cattgaatta
     3241 cggctatcaa tcaacgcaat aaacaaatat tcggtagcta aaaaagtgta acgacacaaa
     3301 ataaaagtgc caccatcggc aaacggatat ttaatcagct ctgctgcaag cattttggca
     3361 cagctatggc taaaaggtaa aaaatcggtt tcttgctcca acaagcggtt gagctgttga
     3421 gcgaaaaccg actccggttt aaatataccg taagcctttg ctttactttg ataggcttga
     3481 tgtaattgca acatcattt
//
LOCUS       DANJWC010000193         3424 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.193, whole genome shotgun sequence.
ACCESSION   DANJWC010000193 DANJWC010000000
VERSION     DANJWC010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.193"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            53..2143
                     /locus_tag="PWX02_002232"
     CDS             53..2143
                     /locus_tag="PWX02_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HDL1730202.1"
                     /translation="MLISLIFSNGICRIPNLEHFLPDCRIIRIPSWRKKLGNKAKVDQ
                     IIGWGLRPSAVGARAYAQKHQLPYIALEDGFLRSLGLGVKGYPPFSLVYDDVGIYYDT
                     TCPSRLEKFLLEQPLQDQEQIKLAEKALSQILTYQLSKYNHALDFSEIPSIKEKIVLV
                     IDQTFGDMAVKYGQASEVDFNKMLETAISENPEAQIWVKTHPDVLSGKKKGYLTDLPQ
                     YADRVRIITEDVNPASLIKWCEKVYCVTSQMGFEALLLGKKVVTFGVSWFAGWGVTDD
                     RHNSVQLLIQQKRRQARSLLQLFYAAYFDYSRYINPNTGEKGSIFDVIEYLHKATILN
                     RRLSGNVYCIGMSLWKQAVIKPFFNLPQCRLHFVRSLNSLKKRPLAENAKILVWSNGK
                     PEIIQFAKENHIPLLRMEDGFIRSVGLGANLVAPLSLVVDDLGIYFNAENPSRLEQIL
                     LEAEDFSENDLSYAEALRQKLVATHIGKYNVGSGEVQVNAQGKKIILVPGQVEDDASI
                     KSGSPQIKRNLDLLKKVRELNPSAYIIYKPHPDVVSGNRVGQIPTAETRRFADQMVDN
                     ANILDCILIADEVHTMTSLAGFEALLRGKKVHCYGLPFYAGWGLTEDRLKSARRGKSL
                     TLSQLIAATLIYYPLYVDPISRQFIDANQAIHILSQQKRNLTNQRIKRSWVSKQYGKL
                     QHLYRAFKYASHTK"
     gene            2166..3398
                     /locus_tag="PWX02_002233"
     CDS             2166..3398
                     /locus_tag="PWX02_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HDL1730203.1"
                     /translation="MEHYLDELIINSQRILLLQGPIGSFFTEFARWLESEQKEVFKLN
                     FNGGDEYFYPNNSPNTFPYYDSLDNFEAYLIQFCQTHQIDNILCFGDNRYYHKVAKNV
                     CLKLKLVFWAFEEGYFRPEYITLEKWGVNAFSPIPKNHTFFLPYVELPVVSEPEKVAK
                     GFRCMAGKAIPYYWNAYKKRKAYAKYKHHRFLNLKYYLNLWTRSGIKRICYWLHDKGF
                     AKRVEKGEFGEFFIVPLQVYDDTQVRIHSEQKSVERFLREVLNSFAKFAPAHLNLIIK
                     HHPMDRGFIDYKMVIYEFLEQYPQLNGRVFYIHDVPLPIFLRYGKGMVTLNSTSGLSA
                     LLHNMPVKTLGRANYDFEGLTDKQPLDTFWANPQPPNSEVFKGYRQFHLHKTHINGNF
                     YHKSILRYPYNWENEAHR"
BASE COUNT         1076 a          545 c          720 g         1083 t
ORIGIN      
        1 tttttgaaaa tttgcagaga acgtttaata aatttaaaga taattattaa gtatgctcat
       61 ttcccttatt ttttcgaacg gaatttgtcg tattccgaat ttagaacatt ttctacctga
      121 ttgtcgtatt attcgaatac cttcttggcg taagaaatta ggtaataagg ctaaggtaga
      181 tcaaatcatc ggctgggggc ttcgtccttc agcggtggga gcaagagctt atgcacaaaa
      241 acaccaactt ccttatattg cattagaaga cggcttttta cgctcattag gcttgggtgt
      301 aaaaggctat ccgccttttt ctctagtgta cgacgatgtc gggatttatt acgatactac
      361 ttgtccttcc cgtttagaaa aattcctgct tgaacagcct ttgcaggatc aagagcaaat
      421 taagcttgca gagaaagctt tatctcaaat cttaacttat cagctttcta aatataatca
      481 cgcattagat tttagtgaaa tcccctctat caaagaaaaa atcgtattag ttattgacca
      541 aacctttggt gatatggcgg tgaaatatgg ccaagcgagt gaagtggatt tcaataaaat
      601 gttggaaacg gcaatcagtg agaatccaga ggctcagatt tgggtgaaaa ctcaccccga
      661 tgtattaagt ggcaagaaga aaggctattt gaccgatttg cctcaatatg ctgatagagt
      721 tcgtattatt actgaagatg ttaatccggc aagcttgatt aaatggtgtg agaaagtcta
      781 ttgcgtaact tcgcaaatgg gctttgaggc attattgtta ggtaaaaaag tggttacctt
      841 tggtgtgtcg tggtttgccg gttggggggt aacagatgat cggcataact cagttcaatt
      901 attaatccaa cagaagagac ggcaagcacg ctcattgtta caactatttt atgcagcata
      961 ttttgattac agtcgttata ttaatccgaa tacgggtgaa aaaggctcta tttttgatgt
     1021 gattgagtat ctgcacaagg cgacaatact caaccggcgt ttaagcggaa atgtatattg
     1081 tataggtatg tcattatgga aacaggctgt cattaagccg ttttttaatt tgcctcaatg
     1141 ccgtttacat tttgtgcgtt cgctaaattc tttgaaaaaa cgaccgcttg cagagaatgc
     1201 taaaatttta gtgtggagta atggtaagcc tgaaattata caatttgcga aagaaaatca
     1261 tattccactg ttgagaatgg aagatggttt tatccgttcg gttggacttg gggcaaattt
     1321 agttgcgcca ctttctttgg tagtagatga tttaggtatt tattttaatg cagagaatcc
     1381 ttcccgtttg gaacaaattt tattggaagc ggaggatttt tctgaaaatg atttgtctta
     1441 tgctgaggca ttaagacaaa aattagtggc cacccatatt ggtaaatata atgtcggtag
     1501 tggtgaggtg caggttaatg cccaaggcaa aaaaataatt ttagtaccgg ggcaggttga
     1561 agatgacgct tcgataaagt ccggttctcc acaaattaaa agaaatctgg atttactgaa
     1621 aaaagtgagg gaattaaacc ctagtgccta tattatttat aagcctcacc ctgatgtggt
     1681 aagtggtaat cgagtcggtc agattcctac ggcagaaaca cgcaggtttg cggatcagat
     1741 ggtggataat gccaatattt tagattgtat tttaattgca gatgaagtgc atacaatgac
     1801 atctttagcc ggtttcgaag cattattaag aggtaaaaaa gtgcattgct acggattacc
     1861 tttttatgct ggttggggat tgacggaaga tagattgaag tcggcacgca gaggtaaatc
     1921 tttgacatta tctcagttaa ttgccgcaac attgatatat taccctctgt atgttgatcc
     1981 tataagcagg cagtttattg atgccaatca agcgattcat atattgtcac aacagaaacg
     2041 aaacctaacc aatcaaagga ttaaacgttc ttgggtgagt aaacaatatg gaaaattaca
     2101 gcatctttat cgtgcgttta aatatgcttc ccacactaaa taagcgtaga gggtaggaaa
     2161 ttaagatgga acattattta gatgagctaa tcatcaatag ccagagaatt ttattgttac
     2221 aaggcccgat aggctctttt ttcactgagt ttgcccgttg gttagaaagt gagcaaaaag
     2281 aggtcttcaa gttaaatttt aatggcggag atgaatattt ctatccaaat aatagcccga
     2341 atacttttcc ttactatgat agcttagata attttgaagc ctatttaatt cagttttgcc
     2401 aaactcatca aatagataat atcttatgtt ttggcgataa tcgttattat cataaagtgg
     2461 ctaaaaacgt ttgcttgaag ttaaagttgg tcttttgggc gtttgaagaa gggtacttta
     2521 ggccagaata tattacgttg gaaaaatggg gtgtaaatgc tttttctcca atcccgaaaa
     2581 atcacacttt ctttttgcct tatgtcgagt taccagtagt cagtgaacca gaaaaagtag
     2641 ctaaaggctt tagatgtatg gcgggaaaag caatcccgta ctattggaat gcctataaaa
     2701 aacgtaaagc atacgcaaaa tataagcatc atcgttttct gaatctcaaa tactacctta
     2761 atttatggac aaggtcggga attaagcgaa tatgttattg gttacatgat aaaggatttg
     2821 ccaagcgggt agaaaaagga gagtttggtg aatttttcat tgtgcctcta caagtttatg
     2881 atgacactca agtgaggatt catagtgaac aaaaaagtgt agagcgtttt ttaagggaag
     2941 tgctgaattc ctttgccaaa tttgcaccgg cacatctcaa tttaattatt aagcatcacc
     3001 cgatggatag aggatttata gattataaaa tggtgattta tgaattctta gagcaatatc
     3061 cccaattaaa cggtagagtt ttttatattc acgatgtgcc gttacctatc tttttacgtt
     3121 atgggaaagg aatggtgact ttaaattcaa ccagtgggct ttctgcattg ctccataata
     3181 tgccggtcaa aaccttaggt agagccaatt atgattttga gggattaacc gataaacagc
     3241 cattagatac tttttgggct aatcctcagc cacctaattc ggaagtattt aaaggctaca
     3301 gacaatttca tttacataaa acgcatatta acggtaattt ttaccataaa tcgattttgc
     3361 gttatcctta taattgggaa aatgaagcac acaggtgatg gaaaatatta aaataagtaa
     3421 tatc
//
LOCUS       DANJWC010000194         3261 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.195, whole genome shotgun sequence.
ACCESSION   DANJWC010000194 DANJWC010000000
VERSION     DANJWC010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.195"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..571)
                     /locus_tag="PWX02_002234"
     CDS             complement(<1..571)
                     /locus_tag="PWX02_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730204.1"
                     /translation="MKKYLMLCSLWFLSACFNESYDFRGKEFEEPVQAILVESDKIYF
                     IGSTIDAIFDKTLIDNTKASDVIYFLNKLLQSPYGRKISNHLITLAIYDENIQIQYSP
                     QFIAKNLTMSDIQALQKLGFQFSHSKEYLWKSYAGLGGKITQFKNRKEILEKYKFKQP
                     IIAKAKSYQKSIEFSFDDTISVIAAPITIP"
     gene            805..1095
                     /locus_tag="PWX02_002235"
     CDS             805..1095
                     /locus_tag="PWX02_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDL1730205.1"
                     /translation="MALRPLHDKVILKREEVETKSAGGIVLTGSAATKSTRGKVIAVG
                     TGRILDNGSVQPLAVKVGDVVIFNEGYGVKTEKIDGEEVLILAEHDILAIVE"
     gene            1164..2804
                     /gene="groL"
                     /locus_tag="PWX02_002236"
     CDS             1164..2804
                     /gene="groL"
                     /locus_tag="PWX02_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608625.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDL1730206.1"
                     /translation="MAAKDVKFGNDARVKMLKGVNILADAVKVTLGPKGRNVVLDRAY
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIVNE
                     GLKAVAAGMNPMDLKRGIDKAVAAVVEELKAISKPCETSKEIEQVGTISANSDSTVGQ
                     LIAQAMEKVGKEGVITVEDGTGLEDALDVVEGMQFDRGYLSPYFINKPEAGTVELENP
                     YILLVDKKVSNIRELLPVLEGVAKAGKPLLIIAEDIEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIAILTAGTVISEEIGMELEKATLEELGQAKRVVISKDNTTIID
                     GIGDETQIKGRVAQIRQQIEDSTSDYDKEKLQERVAKLAGGVAVIKVGAATEVAMKEK
                     KDRVDDALHATRAAVEEGIVPGGGVALVRAANKVADTLKGDNEEQNVGIKLALRAMEA
                     PLRQIVANAGEEASVVARNVKEGSGNYGYNAGTEQYGDMLEMGILDPTKVTRSALQFA
                     ASIAGLMITTECMITDLPKEEKLDPAAMGGMGGMGGMM"
     gene            complement(2929..3255)
                     /locus_tag="PWX02_002237"
     CDS             complement(2929..3255)
                     /locus_tag="PWX02_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5339 domain-containing protein"
                     /protein_id="HDL1730207.1"
                     /translation="MLKRILGITLCTILVSSNAIASVKTSSLTVAKPTLSKNLPTECE
                     KMFNTANKLVSDAERQPGTHTQVLKMKNKLSLTKQQILKMDSELQQKSCEKGLTALNS
                     LKQKHQ"
BASE COUNT         1030 a          523 c          728 g          980 t
ORIGIN      
        1 cgggaatcgt aattggtgct gcaatgaccg aaatggtgtc atcaaagcta aattctatgc
       61 ttttctgata acttttcgct ttagcgatta taggttgctt aaatttatac ttttctagta
      121 tttccttgcg atttttaaac tgtgtgattt taccaccaag acctgcatag cttttccata
      181 gatattcttt tgaatggcta aattgaaaac ctaacttttg tagagcttga atatcgctca
      241 tggtgagatt ttttgcaata aattgcggac tgtactgaat ttgaatattt tcatcataaa
      301 tggctaaagt aattaggtga ttactgatct tccttccata cggactttga agaagtttat
      361 tcaaaaaata aataacatct gaagcttttg tattatctat cagcgtttta tcaaatatcg
      421 catcaattgt ggaaccaatg aaataaattt tatctgattc aaccaaaatt gcttgtacag
      481 gttcttcaaa ttcttttcct ctaaaatcat aactttcgtt aaagcatgcg gagagaaacc
      541 atagtgagca gagcattaag tattttttca ttttattatc ctatcttgaa attaatagtt
      601 taaatggtat aagagaattg gggtttttct aaacagaaac agatttctta ggtatatagt
      661 tttatcaaaa atggtcaaat ttgtaaaaaa ttttacaaat ttgaccgctt gcacttgaaa
      721 ctctaaaatc tgcctttata ttgttaggcg ttacgcattt tgcgtgaagt tttatttatt
      781 catttttaag aaaaggaaca tattatggca cttcgtccat tacacgataa agttatttta
      841 aaacgtgaag aagttgaaac aaaatcggca ggcggtattg ttttaaccgg ttcggcagca
      901 acaaaatcaa cccgtggcaa agtaattgca gtaggaacag gtcgcatttt agacaatggt
      961 tctgttcagc ctttagcagt taaagtgggc gatgtagtga tttttaacga aggctacggc
     1021 gtaaaaaccg agaaaattga cggcgaagaa gtgctaattt tagcagagca cgatattttg
     1081 gcgattgtag aataatttcg ttctgcacaa gcggttaaaa aagcaaaatt ttttacatat
     1141 ttcaaataca aaggatttag aaaatggcag caaaagacgt gaaatttggt aacgatgcac
     1201 gcgtaaaaat gttaaaaggc gtgaatattt tagccgatgc ggtaaaagta actttaggtc
     1261 cgaaaggtcg taatgtggtg ttagatcgtg cttatggtgc accaacgatt acaaaagacg
     1321 gtgtgtcggt tgcccgtgaa atcgaattag aagataaatt tgaaaatatg ggggctcaaa
     1381 tggtgaaaga ggttgcctct aaagccaatg atgccgccgg tgacggtaca actaccgcaa
     1441 ctgtattagc tcaagcgatt gtaaacgagg gcttaaaagc agtggctgcg ggtatgaacc
     1501 caatggattt aaaacgtggt atcgataaag cggttgcagc agtggttgaa gagttaaaag
     1561 cgatttcaaa accgtgcgaa acctcaaaag aaattgagca agtagggacg atttctgcaa
     1621 actcagattc aacagtcggt caattaattg ctcaagcaat ggaaaaagtg ggcaaagagg
     1681 gcgtgattac tgttgaagac ggtacaggtt tagaagatgc attagacgtg gttgagggta
     1741 tgcaatttga ccgtggttac ttatcgccat actttatcaa taaaccggaa gccggtacgg
     1801 ttgagttaga aaacccatat attcttttag tggataaaaa agtgtctaac attcgtgaat
     1861 tattaccggt gttagaaggt gttgcaaaag caggtaaacc gttattaatt attgccgaag
     1921 atattgaagg cgaggcgtta gctaccttag tggtgaacac tatgcgtggt atcgtgaaag
     1981 tggcggcagt gaaagcacct ggctttggtg atcgccgtaa agcaatgtta caagatattg
     2041 cgatcttaac tgcaggtact gtgatttctg aagagatcgg tatggagctt gaaaaagcaa
     2101 cccttgaaga gcttggtcaa gcaaaacgtg tggttatcag caaagataac accacaatta
     2161 ttgacggtat tggtgatgaa actcaaatca aaggtcgtgt agcacaaatc cgccaacaaa
     2221 tcgaagattc aacttcagat tacgacaaag aaaaactaca agagcgtgta gcaaaattag
     2281 ccggtggtgt tgcggtaatt aaagtaggtg cggcaactga agtggctatg aaagagaaaa
     2341 aagaccgtgt tgatgatgct cttcacgcaa cccgtgctgc ggtggaagaa ggtattgtac
     2401 cggggggcgg agttgcttta gtgcgtgcag ccaacaaagt ggctgataca ctaaaaggcg
     2461 ataacgaaga gcaaaatgta ggtatcaaac ttgccttgcg tgcaatggag gctccattac
     2521 gccaaatcgt tgccaatgca ggtgaagaag cctcagttgt agcccgcaac gtgaaagaag
     2581 gttcaggcaa ctacggctat aacgcaggta cagagcagta cggcgatatg cttgaaatgg
     2641 gaattttaga cccaactaaa gtgactcgtt cagcacttca attcgcggct tctattgcag
     2701 gtttaatgat cactacagaa tgtatgatta ccgatctgcc aaaagaagag aaattagatc
     2761 ctgccgcaat gggcggtatg gggggaatgg gcggaatgat gtaattgccc attgagtgaa
     2821 caaaagctac tgaaattaat tcagtagctt ttttgttggt aacaagtggt taaatttatg
     2881 ctattttttg caaataaaaa accaagcaag taagcttggt tttagcgttt attgatgttt
     2941 ttgtttaagg ctgtttagtg ctgttaaacc tttctcacaa cttttttgct gtaattcaga
     3001 atccattttt aggatttgtt gctttgttaa agaaagttta tttttcattt ttagcacttg
     3061 agtatgagta ccaggctgtc tttctgcatc agagaccaat ttatttgccg tgttaaacat
     3121 tttctcacat tcagttggca aatttttact taaagtaggt tttgcaactg taagtgatga
     3181 ggttttaaca cttgctatag cattactgga aaccaatatg gtacaaagtg ttattcctaa
     3241 aattcttttt agcatgcgtt c
//
LOCUS       DANJWC010000195         3228 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.196, whole genome shotgun sequence.
ACCESSION   DANJWC010000195 DANJWC010000000
VERSION     DANJWC010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.196"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..1329
                     /locus_tag="PWX02_002238"
     CDS             <1..1329
                     /locus_tag="PWX02_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HDL1730208.1"
                     /translation="GLPLLFLDYSIGHKYRGGAPLSFRRFSSKFETFGWWQVLINVII
                     GIYYAVVLGWAASYTYFSFSGAWGDKPVDFFIGEFLKMGDITQGVSFEFVGMVTGPLI
                     AVWLIAIAVLAMGIKKGISAASNLLMPLLIVMFLVLVGYSLTLPGAAKGLDALFTPNW
                     EKLADPSVWIAAYGQIFFSLSICFGIMITYASYLKKETDLTSSGLVVGFANSGFELLA
                     GIGVFAALGFMATAAGQEVNEVAKGGIGLAFFAFPTIINQAPMGALVGVLFFGSLTFA
                     ALTSFISVIEVIISAVQDKLNSGRVKATLLVGIPMMVVSVLLFGTTTGLPMLDVMDKF
                     VNQFGIVAVAFVSLIAIVTSEKLSTLEKHLNEVSSFKVGFIWRLCIVITSGVLAFMLF
                     SEIAKVYREGYEGYPSWFVNTFGWGMAIGLVVVAYLLSKMAWKNEPKGDE"
     gene            1329..1427
                     /locus_tag="PWX02_002239"
     CDS             1329..1427
                     /locus_tag="PWX02_002239"
                     /inference="COORDINATES: protein motif:HMM:NF028261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HDL1730209.1"
                     /translation="MSSAAIITMVVAMTIIWGGLLFAIKRLPKEEQ"
     gene            complement(1475..1738)
                     /locus_tag="PWX02_002240"
     CDS             complement(1475..1738)
                     /locus_tag="PWX02_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxA family glutaredoxin"
                     /protein_id="HDL1730210.1"
                     /translation="MFVEIYGRLSCPYCVRAKTLAEKMKQELSDFDFKFIDMIAENIS
                     KQDLEPRVGKPVATVPQIFVDNNHVGGCTDFQAFVEEKFGITV"
     gene            1898..>3228
                     /gene="srmB"
                     /locus_tag="PWX02_002241"
     CDS             1898..>3228
                     /gene="srmB"
                     /locus_tag="PWX02_002241"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HDL1730211.1"
                     /translation="MTQLMTFEELDLSPQLLKALAHKGYNRPTAIQQATIPAALEGRD
                     LLGSAPTGTGKTAAFLLPAIQHLLDYPRCKPGAPRILILTPTRELAMQVAEQAQALAK
                     FTQLSIATITGGVAYQNHGEVFNKNQDIVVATPGRLMQYIKEENFDCRAVEVLIFDEA
                     DRMLQMGFGQDAEKIAAETRWRKHTWLFSATLEGELLEDFAERLLNDPIKVDAEPSRR
                     ERKKIQQWYYHADNVEHKTKLLARIISEFSVERAIVFVRRREDVRELSETLRKRGIRS
                     TYLEGEMAQTQRNQAINRLKEGVVNVLVATDVAARGIDIDDIDFVLNYDLPYSADTYL
                     HRIGRTARAGKKGSAISLVEAHDYKLLGKIKRYTEEVLKPRIIEGLEPRTKAPKDGEL
                     NVTSKKEKARIKKKKEAKKEAAKPKVKVRHKDTKNIGKRRKPSSESAAVKQA"
BASE COUNT          896 a          655 c          778 g          899 t
ORIGIN      
        1 ggtttaccgc tgctgttctt agattattct atcgggcata aatatcgtgg tggtgcaccg
       61 ctctctttcc gccgtttcag ctcgaaattt gaaaccttcg gttggtggca agtgctgatc
      121 aacgtgatca tcgggattta ttatgcggtg gtactcggct gggcagcaag ttacacctac
      181 ttctcattca gcggagcgtg gggcgataaa ccggtggatt tctttatcgg tgaattctta
      241 aaaatgggcg atattactca aggcgtgagc tttgaatttg tcggtatggt tacagggccg
      301 ttaattgcgg tatggctgat tgctattgct gtgttggcta tggggatcaa aaaagggatt
      361 tctgctgcct caaacctgct aatgccgctg ttgatcgtga tgttcttagt gttggtcggc
      421 tattcattaa ccctaccggg tgctgcgaaa ggcttagatg cgttatttac cccaaactgg
      481 gaaaaattgg ctgatccaag tgtgtggatt gccgcttacg gtcaaatctt cttctcactc
      541 tctatctgct tcgggattat gattacctat gcttcatacc tgaaaaaaga aaccgattta
      601 accagttcag gcttagtggt gggttttgcg aacagtggtt ttgaattatt agcgggtatt
      661 ggcgtatttg cggcactcgg ctttatggca acggctgcag ggcaagaagt gaacgaggta
      721 gcaaaaggcg gtatcggcct ggcattcttc gctttcccaa ccattattaa ccaagcacca
      781 atgggggcat tagttggcgt gctgttcttt ggttcactca cctttgcggc attaacctca
      841 tttatctcgg taattgaagt gattatttca gcggtgcaag acaaactcaa ttcaggtcgt
      901 gtgaaagcca cgctactagt cggcattccg atgatggtgg tatctgtctt gctattcggt
      961 acaaccaccg gtttaccaat gcttgatgtg atggataaat tcgtaaacca attcggtatt
     1021 gttgcggtgg cgtttgtttc cttgattgcg attgtcacca gcgagaaatt aagtaccctt
     1081 gaaaaacacc tgaacgaagt ttcttcattc aaagtcggct ttatctggcg tttatgtatc
     1141 gttattacct ccggcgtact ggcatttatg ttattcagcg aaatcgccaa agtgtatcgt
     1201 gaaggttatg aaggctaccc aagctggttt gtaaacacct tcggctgggg aatggcaatc
     1261 ggcttagtgg tggtggctta tttgctatca aaaatggcgt ggaaaaatga accaaaagga
     1321 gatgagtaat gagttcagca gcaattatta ccatggtggt ggcaatgacg attatctggg
     1381 gcggtttact ttttgcgatt aaacgtctgc cgaaagaaga acaataattt gcaaattttt
     1441 gtataaaaaa gcccgcttgt tagcgggctt ttggctatac ggtaatccca aatttctctt
     1501 ccacaaaggc ttggaaatcg gtgcaaccgc caacgtggtt attatccaca aaaatttgtg
     1561 gcacagttgc aaccggcttg ccaacacgag gctctaggtc ttgttttgaa atattttcag
     1621 cgatcatatc aataaattta aaatcaaaat cgcttaattc ttgtttcatt ttctcggcta
     1681 atgtttttgc acgcacgcag tatgggcaag ataaacgtcc ataaatttca acaaacatat
     1741 aatgttcctt ctataaagta aaagtgagcg aattgtccgt taaacggctc tgtgtaagtg
     1801 taacaatttt ttcagccttt taggataaat ttcttcacca tcacggcaag cagttaatgt
     1861 gatatactgg ggcaaattta caagtagaga aacagagatg acccaattaa tgacgtttga
     1921 ggagcttgat ttaagccctc aattacttaa agccttagca cacaaaggct ataaccgccc
     1981 aaccgctatt caacaagcca cgattccggc tgcgttagaa ggacgtgatc tgctcggttc
     2041 agcccctacc ggtaccggca aaaccgccgc ttttttactg ccggcaattc agcatttatt
     2101 agattatcct cgctgcaaac caggggctcc tcgcatttta attcttaccc caacccgaga
     2161 gctggcaatg caggtggcgg agcaagccca agcactggct aaatttactc agttgagcat
     2221 tgcgactatt accggtgggg tggcgtatca aaatcacggt gaagtcttta ataaaaatca
     2281 agatattgtg gttgctaccc cgggtcgttt aatgcaatac attaaagagg aaaattttga
     2341 ctgccgagcg gtggaagtgc tgatttttga tgaagcagat agaatgttgc aaatgggctt
     2401 cgggcaagat gcggagaaaa ttgcggcaga aacccgttgg cgtaagcata cttggttgtt
     2461 ttcagcgacc ctcgagggcg agttattaga ggattttgcc gagcgattat taaatgatcc
     2521 gattaaagtc gatgccgaac caagtcgccg tgagcgtaaa aaaattcagc aatggtatta
     2581 ccacgccgat aatgtagagc ataaaaccaa attattagct cgtattatca gcgaattttc
     2641 ggtggaaaga gcgattgtgt ttgtccgccg ccgagaagat gtgcgagaac tcagcgaaac
     2701 cttgcgtaag cggggaattc gttctactta tctcgaaggt gaaatggctc agacccaacg
     2761 taaccaagct attaaccgtt taaaagaagg cgtagtgaat gtgttagtgg caaccgatgt
     2821 ggcggctcga ggcattgata tagacgatat tgatttcgtg ctgaactacg atttacctta
     2881 tagtgcggat acttacttgc accgtattgg gcgtactgct cgtgctggca aaaaaggttc
     2941 ggcaatttca ttggtggaag ctcacgatta taaattactc ggcaaaatta aacgttacac
     3001 cgaagaagta ctaaaacctc gcattatcga gggcttagag cctcgcacca aagccccgaa
     3061 agatggcgag ctaaatgtga ccagcaagaa agaaaaagca cgcattaaga agaaaaaaga
     3121 ggcgaaaaaa gaagcggcaa aaccaaaagt aaaagtccgc cacaaagata ccaaaaatat
     3181 cggtaaacgg cgtaaaccaa gtagtgaatc ggctgcggtt aaacaggc
//
LOCUS       DANJWC010000196         3228 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.197, whole genome shotgun sequence.
ACCESSION   DANJWC010000196 DANJWC010000000
VERSION     DANJWC010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.197"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..569)
                     /gene="pdxT"
                     /locus_tag="PWX02_002242"
     CDS             complement(<1..569)
                     /gene="pdxT"
                     /locus_tag="PWX02_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HDL1730212.1"
                     /translation="MKIAILALQGAFSEHTDKLQQLGVESMEIRQLADLNQDFDGLIL
                     PGGESTVQGKLLRELGLLEPLRQKILNGLPTLGTCAGLILLAENLVNDDKKHLATLPV
                     TVKRNAYGRQLGSFLVEEEVKYLGKIPLTFIRAPYIESTGKDVEILAKISGKIVGVKY
                     QNQIGISFHPEVNNDLSFHRYFVEMCRKN"
     gene            complement(569..1441)
                     /gene="pdxS"
                     /locus_tag="PWX02_002243"
     CDS             complement(569..1441)
                     /gene="pdxS"
                     /locus_tag="PWX02_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HDL1730213.1"
                     /translation="MTNRYDLNRQLAQMLKGGVIMDVTTPEQACIAEEAGACAVMALE
                     RIPADIRAAGGVSRMSDPKMIKSIQAAVSIPVMAKVRIGHVTEAQILQAIEIDYIDES
                     EVLSPADDTFHINKRKFEVPFVCGAKNLGEALRRINEGAAMIRSKGEPGTGNVVQAVH
                     HLRKMKQEIARLVSHTTDELYHVAKELQVPFDLVQYVHQYQKLPVVTFAAGGIATPAD
                     AALMMQLGAEGVFVGSGIFKSGDPAKRARAIVQAVTNYNDPKLLAELSEDLGEAMVGI
                     NEQEIELLMAARGV"
     gene            1652..3055
                     /locus_tag="PWX02_002244"
     CDS             1652..3055
                     /locus_tag="PWX02_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL1730214.1"
                     /translation="MLTYTFNTQEGTALYEQLYRFLKNDIESNILIAGDKLPSKRAFA
                     KHLGISVMTVETAYQQLVAEGYVNTQPKKGFFVNALNLPEKKEAISVQLQKISSEQYE
                     KNKKWQADLTNRQTLAENFPFSVWTKLVREVLKTHQSELMERAESGGVWTLKFAIANH
                     LRAFRGMIVNPTQIIIGAGTEYLYGLLIQLLGLDKHYALTEPSYDKLRRIYHSYRLST
                     ATVSMPFSIDELNSKNVDVIHTSPSHHFPTGHVMPIAKRYELLAWVAQKAERYIIEDD
                     YDSEFRFVGQPIPALKSIDMLGRVIYMNTFSKTLSSTVRIAYMVLPPELLNRFQQELG
                     FYASTVSNFEQYILAEFIQQGYFEKHINRMRAYYQKKRDKLLKAFKQGNLAEKIAIKE
                     EHSGLHFIVQLNTTLTERQILQAAEEQGIKFVALSAYYQDKSQIQQNAFIIGYSNLKD
                     DQINYVAESLKKILDNE"
BASE COUNT         1006 a          640 c          592 g          990 t
ORIGIN      
        1 ttattttttc ggcacatttc cacaaaatag cggtgaaaac ttaaatcatt attcacttcg
       61 ggatggaagg atatacctat ctgattttgg tattttactc caacaatctt accgcttatc
      121 ttggctaaaa tttctacatc ttttccagta ctttctatat aaggcgcacg aatgaatgtg
      181 agaggaattt taccgagata tttaacttct tcttctacta aaaacgagcc taattgcctt
      241 ccgtaagcat tacgtttcac tgttacaggc aaagtagcca aatgtttttt atcgtcgttt
      301 accaaatttt ccgcaagcaa aattaaaccc gcacaagttc caagcgttgg caagccattt
      361 aaaatttttt gacgaagcgg ctcaagcaag cctaattcac gtaataattt accttgaaca
      421 gtcgattcgc caccaggtaa aatcagacca tcaaaatctt ggtttaaatc agcaagttga
      481 cgaatttcca tactttcaac ccctagttgc tggagtttat cagtatgctc gctaaatgca
      541 ccttgcagag caagaatagc aattttcatt agacacctct tgctgccatt agcaattcaa
      601 tctcttgctc attaattcct accattgctt cacctaaatc ttcagaaagc tccgcaagta
      661 atttaggatc gttgtagttg gtaacagctt gtacaatggc ccgcgctcgt ttagcaggat
      721 cgcctgactt gaaaatacct gagccaacga agactccttc tgcacctaac tgcatcatca
      781 atgcagcatc ggcaggggtt gcaatacctc cagcagcaaa ggtgacaaca ggcaattttt
      841 ggtactgatg tacatattgt actaaatcaa atggtacttg gagctcttta gctacgtgat
      901 aaagttcatc tgtggtatgt gaaaccaagc gggcaatttc ttgtttcatt ttgcgaagat
      961 gatgtactgc ttgcaccaca ttgcctgttc ctggctcgcc cttactacgg atcatagcag
     1021 cgccttcatt aatacgacgt agggcttcgc ctaaattttt tgctccacat acaaatggca
     1081 cttcaaattt acgcttgtta atgtggaagg tatcatctgc aggagaaagc acctcacttt
     1141 cgtcaatata gtcaatttcg atcgcttgca ggatttgtgc ttctgtaacg tgtccaatac
     1201 gtactttcgc cattacagga atggataccg ctgcttggat actctttatc atcttgggat
     1261 cactcatacg agaaacgcca ccagctgcac gaatatctgc aggaattctc tctagtgcca
     1321 ttactgcaca agctccagct tcttccgcaa tgcaggcttg ctcaggggta gttacatcca
     1381 taattacacc gccttttaac atttgagcaa gttgtctgtt taaatcatag cgattagtca
     1441 ttgtactttt tccttctaat tgttgaataa ttgaactttt cgtagtttcg attagctcgg
     1501 cattaagttt atttttatat tcgctggatg cgagtgttca tctaccaatt taaatacact
     1561 cagccaattt gagtgaggaa acagcatgta ttgtaattta atctgaactg tttaaaatga
     1621 ccattttatg tttttcttat catgtcagat tatgcttacc tacactttta acacacaaga
     1681 aggcaccgct ctctatgagc aactttaccg ttttctaaaa aacgacattg aaagcaacat
     1741 acttatagca ggggataaac tgccgtctaa acgggctttt gcaaagcatt taggcatttc
     1801 agtaatgaca gtggaaacag cttaccaaca gttagtagca gaaggttatg tgaatactca
     1861 gcctaaaaaa ggcttttttg tgaatgcttt aaatttacca gaaaaaaagg aagccatatc
     1921 agttcagcta caaaaaatca gttcagaaca gtatgagaaa aataaaaaat ggcaggcaga
     1981 tttaactaat cgacagactt tagcagaaaa ttttccgttt tcggtttgga caaaattagt
     2041 acgcgaagtg ctgaaaacac accaaagtga gctaatggag cgtgctgaga gtgggggtgt
     2101 atggacatta aaatttgcga tagccaatca tcttcgagcc tttagaggaa tgatcgttaa
     2161 tccaacacag attattattg gagcgggtac ggagtatctt tacggtttgc tcattcagct
     2221 tctaggctta gataaacatt atgcactaac tgagcccagt tacgataaat tgcgcagaat
     2281 ttatcacagt tatcgcttat caacagccac agtatcgatg ccattttcaa tcgatgagtt
     2341 aaattcaaag aatgtggacg tgatacatac ctcgccatca caccattttc cgacaggaca
     2401 cgtaatgccg attgccaaac gttatgagtt gttagcttgg gttgcacaaa aagctgaaag
     2461 atatattata gaagacgact acgacagcga atttcgtttt gtaggacaac caattcctgc
     2521 attgaaatct attgatatgc tcgggcgagt tatctatatg aatacttttt ctaaaacgct
     2581 ctcttcaact gtacgaattg cctatatggt tttgccacca gaattactga atcgttttca
     2641 gcaggaatta gggttttatg cttctaccgt ttccaatttt gagcaatata ttttggcaga
     2701 atttattcaa caaggctatt ttgaaaagca tatcaatcga atgcgagcgt attatcaaaa
     2761 aaagagggat aaattactaa aagctttcaa gcaaggcaat ttagcagaaa agattgcaat
     2821 aaaagaagaa cattcaggct tgcatttcat tgtacagcta aatacaacat taacagaaag
     2881 gcaaattttg caggcagcag aagaacaagg tataaaattt gtcgcattgt ctgcttatta
     2941 tcaagataaa tctcaaattc agcaaaatgc ttttataatt gggtactcta atttaaaaga
     3001 tgatcagatt aattatgtag cagagagcct gaaaaaaatt ttagacaacg aatagcaagt
     3061 atcaaaactg ctttaatttt tagcaaattg ccttaaaaac acgcaagtgg taattatttt
     3121 agtgattttt gcaaaaaatt gccaaattct ccttgcaagg cattttgttt tgcgtataat
     3181 gcatggcaca caacgacgca ctgttgtgaa catttaaaag ttgaattc
//
LOCUS       DANJWC010000197         3215 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.198, whole genome shotgun sequence.
ACCESSION   DANJWC010000197 DANJWC010000000
VERSION     DANJWC010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.198"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            296..3208
                     /gene="rapA"
                     /locus_tag="PWX02_002245"
     CDS             296..3208
                     /gene="rapA"
                     /locus_tag="PWX02_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HDL1730215.1"
                     /translation="MFLVGQRWLSESENNLGLGIVTSVDHRTVTINFPAAEEERVYAI
                     AVAPLTRVQFQAGDRINSIDGWQLEVSEVVENQGAVIYLGKRVDNGEEAVLPEMLLDH
                     KISFSKPQDRLFSAQIDRSDRFALRFNALQHQQAQYQSPLRGLRGIRASLIPHQLHIA
                     KEVGQRLAPRVLLADEVGLGKTIEAGMILQQQLFSGRAERVLVLVPESLQHQWLVEML
                     RRFNLKFSLFDEERCSDFDKQDENGDDVSENPFESEALVITSLNWLETSPQRANQVLA
                     ADWDLLIVDEAHHLEWSETESSIGYQLVERLSQQIPAVLLLTATPEQLGQESHFARLA
                     LLDSDRFYDYAEFVQEQAQYKPVADAVSSLLSDKPLSNDEQNAIAELLSEQDTEPMFR
                     AINSKKSDENDRLQARQELISELIDRHGTSRVLFRNTRQGVKGFPHREYHQISLEMPK
                     QYQNALKVMGMMGSVSQSDLLYPEYLFQRMNPNAKWAEFDPRIDWLITFLKNHRDEKV
                     LVICKQADTAIALEQVLREREAIRSAVFHEKMSIVERDKAAAYFAQQEEGAQVLISSS
                     IGSEGRNFQFAKDLVLFNLPDNPDLLEQSIGRLDRIGQKNDIQIYVPCFNLSAQQMLA
                     EWYHKGLNAFEETCPMGTAIFRELGEELQQFLQKSELSAQFDDFVAKTYKRQQYLKAE
                     LEKGQDRLLELNSNGGEAAQQLANDIAKEDNNPQLVNFALSLFDVIGVEQEDLGEQSI
                     VISPTGHMLVPDFPGLSEDGTTITFNRELALMREEVEFLTWDHPMIRNGIDLITSGDI
                     GKSAISLLVNNKLPAGTLLLEALYVVEAQAPKGLNLTRFLPPTPIRVLVDNKGNNIAP
                     QVSFAGLERQLKPVNKQMANKIAKMARADIEKLIKASEKAVEPQVQQVIETAKFEANS
                     KLKAELHRLESLKMVNKNIRADEVEALEQQLNASLAQLETANYRLDSLRVIVSNKA"
BASE COUNT          965 a          611 c          782 g          857 t
ORIGIN      
        1 gtagaatctg atattcatag agatgatgct gatttaaaat tcaaacgtgg tgaccaagag
       61 ctggatattg aaattgaagt aaaaggtaga aatcggattg aatataaggc agatctagat
      121 ttagataaaa actaaattta ccccttaaaa cgaccgcttg tgtgatttaa ccgcacaagc
      181 ggtcttttta ttgtgctttt ttgcaaaaat actcaagccg accggtcccg gaatttggta
      241 aaatagccgt ttaaatgaca ataatgatgc ttttacttat tacttagagg aaaaaatgtt
      301 tttagtcggt cagcgttggc taagtgaatc ggaaaataat ttaggtttgg gaatcgtgac
      361 ttcggtggat catcgcactg tcactatcaa tttcccggca gcggaagaag agcgggtcta
      421 tgcaattgca gttgcacctt taactcgagt gcagtttcaa gccggagata gaattaacag
      481 tatagatggc tggcagttag aggtttcaga ggttgtggaa aatcaaggtg ccgtaatcta
      541 tttgggcaaa agggtggata acggtgaaga agcggtactg cctgaaatgt tgctcgatca
      601 taaaatcagt ttcagcaaac cgcaagaccg ccttttttcg gctcagattg atcgctcaga
      661 tcgctttgca ttacgcttta acgctttgca gcaccagcaa gcgcaatatc agtcgccact
      721 gcgtggtttg cgaggcattc gagccagcct gattccacat caattacata ttgcaaaaga
      781 agtcgggcaa cgccttgctc ctcgtgtgct attagccgat gaagtgggac tgggtaaaac
      841 tattgaagcc ggtatgattt tgcaacaaca gctattttcg ggcagagcag aacgagtatt
      901 ggtattagtg ccggaaagtt tgcaacacca atggttggtg gaaatgcttc gccgttttaa
      961 tctgaaattc tcgttattcg atgaagaacg ttgtagtgat tttgataaac aagatgaaaa
     1021 tggcgatgat gtaagtgaaa atccatttga gagcgaagcg ttagtcatta cttcactaaa
     1081 ctggctggaa acctcaccgc aacgagccaa tcaagtgctt gctgccgact gggatctatt
     1141 aattgttgat gaagctcacc atttagaatg gtcggaaacc gaatcttcta tcggctatca
     1201 gttggtagag cgtttatctc aacaaattcc tgccgtcttg cttttaacgg caacaccgga
     1261 gcaattaggg caagagagcc attttgcccg cttggcgttg ttagattcgg atcgttttta
     1321 tgattatgcc gagtttgtac aggagcaagc acagtataaa ccggtggcag atgcggtgag
     1381 tagcttatta agcgataaac cgctaagtaa tgacgagcag aatgcgattg cggagctgtt
     1441 aagcgagcaa gataccgagc cgatgttccg tgcaattaat agcaaaaaat cggacgaaaa
     1501 tgaccgcttg caagcccgtc aagagctgat tagtgagcta attgaccgcc atggaaccag
     1561 ccgggtgttg tttagaaata cccgtcaggg agtgaaaggc ttcccacatc gggaatacca
     1621 ccaaatcagc ctagaaatgc cgaaacaata ccaaaatgcg ttaaaagtga tgggaatgat
     1681 ggggagtgtg agccaaagtg atttgctcta tcctgaatat ttgttccaac gaatgaaccc
     1741 gaatgcgaaa tgggctgaat tcgaccctcg gattgattgg ctcatcactt tcttaaaaaa
     1801 tcatcgtgat gaaaaagtgc tggtgatctg caaacaggcg gacactgcga ttgctttgga
     1861 gcaagttttg cgtgagcgtg aggcgattcg ttctgccgtt ttccacgaga aaatgtcgat
     1921 tgttgaacgt gacaaagctg ccgcctattt tgctcaacaa gaagaaggcg ctcaggtgct
     1981 gattagttca agcatcggtt cagaagggcg aaatttccaa tttgctaaag atttagtgct
     2041 gtttaatttg ccggataacc cggatttatt ggagcaaagt attgggcgat tagatcgtat
     2101 cggacagaaa aatgacattc agatttatgt gccttgcttt aatttatcgg ctcaacagat
     2161 gttggcagag tggtatcaca agggcttgaa tgcgtttgaa gaaacctgcc cgatgggaac
     2221 agcgatcttc cgtgaattag gtgaagaatt gcaacaattc ctgcaaaaat ctgaactttc
     2281 agcccaattt gatgattttg ttgcgaaaac ctataaacgc caacagtatt tgaaagccga
     2341 actggagaaa ggacaggaca gattattaga actgaattct aatggcggag aagcagctca
     2401 acagctcgct aatgatattg cgaaagaaga taataatccg cagttggtta attttgcctt
     2461 gagtttgttt gatgtgattg gcgttgaaca ggaagattta ggcgagcaga gcattgtgat
     2521 tagcccaacc ggacatatgc ttgtgcctga tttcccgggg ctatcggaag atggcacgac
     2581 cattaccttt aatcgtgaac tggcgttaat gcgggaagag gtggaatttc tcacttggga
     2641 tcacccgatg atccgcaatg gtattgattt aatcacctcc ggcgatattg gcaaatcagc
     2701 aatctcattg ctggtgaata ataaattgcc tgccggtaca ttattacttg aggcgcttta
     2761 tgtggtggaa gcacaagcac cgaaagggct aaatttaacc cgtttcctac cgccaacccc
     2821 gattcgggtt ttagtggata acaaaggcaa caatattgct ccgcaggtct cttttgccgg
     2881 gcttgaacgc caattaaaac cggtgaataa acagatggca aataaaattg ccaaaatggc
     2941 tcgagcggat attgaaaaat taattaaagc gagtgaaaaa gcggtagaac ctcaggtcca
     3001 acaggtgatt gaaactgcca aatttgaggc aaatagcaaa ttaaaagcag aattacaccg
     3061 cttggaatca ctcaaaatgg tgaataaaaa tattcgagcc gatgaagttg aagccttaga
     3121 acaacagctt aacgcttccc ttgcccaact tgaaacggca aattatcgct tagatagctt
     3181 gcgagtgatt gtaagtaata aagcgtaatt ttttg
//
LOCUS       DANJWC010000198         3197 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.199, whole genome shotgun sequence.
ACCESSION   DANJWC010000198 DANJWC010000000
VERSION     DANJWC010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.199"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            48..1238
                     /locus_tag="PWX02_002246"
     CDS             48..1238
                     /locus_tag="PWX02_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HDL1730216.1"
                     /translation="MSNNNAKNSNLSNAKKAKSDEFYTQYHDIEKEMNAYLDFDPDVF
                     RGKTILLPCDDPEWSNFTLYFAQNFARLGLKKLISTSFAHSSKPLNIPYQPSLFESED
                     SQFNALISPLKGKIFTLDKDLNNDGKIDHHDLAWKYLKSDGDFRSDEIKALRDEADII
                     ITNPPFSLFREFITWIMEADKKFAIIGNMNAITYKEVFPLIKDDKIWLGKGFTRSCAH
                     FFSPYESDSEYLELDRFEKLGIAVPKGLFVTRVASIHWFTNIEHGRRHQPLQLMTMTD
                     NLKFNKKLDGKNAYQQYDNYNAIEVPETKAIPSDFDGVMGVPISFLDKYCPEQFEILG
                     ATQRGCHDEVPDTKKYDDYWEMKQNGEKTGSGGGKTNENANLATNDGKKIISSILYCS
                     FYSL"
     gene            1353..1775
                     /locus_tag="PWX02_002247"
     CDS             1353..1775
                     /locus_tag="PWX02_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005579070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF417 family protein"
                     /protein_id="HDL1730217.1"
                     /translation="MRALIHTFRSTTLDITILRLSVCLIFFTFGMTKWFDFEVEILKP
                     MISPTWLNFLYEWLGYHGTSYFLGVVEGIAYIALAVGYWKPRWGIIGSMTVLLVSSVT
                     LSLLLQLGFNSFIFKDILLIGAGLVLLKYDLNRLYPHE"
     gene            2007..3170
                     /locus_tag="PWX02_002248"
     CDS             2007..3170
                     /locus_tag="PWX02_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDL1730218.1"
                     /translation="MQTKLNTEITIEKLCEGFQYNELEGKGLFGLNGKLTIQPEYQRN
                     YIYADGKKDVAVIHSILKGYPLGLIYFNQTDDDKFEVLDGQQRITSIGRFVTNKFAIL
                     DQNNIPCYFSSLPEDKRQFILNTTLLIYECKGTESEIKEWFKTINIAGVPLNEQELLN
                     AVYSGSFVTLAKAEFSNSQNANVQKWSAFVKGSVIRQDFLACALNWVSKGNISDYMSR
                     HRFDENIAELKTYFTTVIDWVNTTFFDAKKEMCGLEWGRLYENYGKQAYDLSKINAEI
                     DRFYDDPCVKNHKGIWEYVLGGMTDKRLLDIRIFDDKTKKDTYSKQTEKAKVANHSNC
                     PLCAVSDNNNKTKIWTLKEMEADHVSAWSKGGLTGAENCEMLCISHNRAKGNK"
BASE COUNT         1060 a          540 c          620 g          977 t
ORIGIN      
        1 ctctttattc caactcgcca actctttttt taacttggga ggttggaatg tcgaataata
       61 atgctaaaaa tagcaatctt tctaacgcta aaaaagccaa aagtgacgaa ttttacacac
      121 aatatcacga cattgagaaa gagatgaatg cttatcttga ttttgatcct gatgtatttc
      181 gtggcaaaac catcttgttg ccttgcgatg atcctgaatg gagtaatttt acactttatt
      241 ttgcacaaaa ttttgccaga cttggtttaa aaaaattaat tagtaccagc tttgctcata
      301 gtagcaaacc tctcaatatc ccctaccaac caagcttatt tgaaagtgaa gattcacaat
      361 ttaatgccct aatttcacca cttaaaggca agatttttac gcttgacaaa gatctgaaca
      421 atgacggcaa aatcgatcac cacgatttag cgtggaaata tttaaaaagc gatggcgatt
      481 ttcgctctga tgaaattaaa gccctacgag atgaagccga catcattatt actaatccgc
      541 cattttctct atttcgtgaa tttattacgt ggattatgga agcggataag aaatttgcga
      601 ttattggcaa tatgaatgcg attacttata aagaagtttt cccattgatt aaagatgaca
      661 aaatttggtt agggaaagga tttactagaa gttgtgcaca tttttttagt ccgtatgaaa
      721 gtgatagcga atatctagag ttagatagat ttgaaaaatt aggcatagct gttccgaaag
      781 gattatttgt tactcgagta gcaagcattc actggttcac caatatagaa cacggtcgcc
      841 gtcatcaacc attacaatta atgacaatga cggataatct caaattcaat aaaaaactag
      901 acggtaaaaa tgcctatcaa caatacgata attacaatgc aattgaagta cctgaaacca
      961 aagcaatacc gagtgatttt gacggagtta tgggtgtgcc gatctcgttc ttggataagt
     1021 attgcccaga gcagtttgag attttgggag caacgcaacg tggttgccac gatgaagtgc
     1081 ctgacacaaa gaaatacgat gattattggg aaatgaaaca gaatggagaa aaaacaggat
     1141 caggcggagg gaaaacgaat gaaaatgcca atttagcgac aaatgatgga aaaaaaatta
     1201 tttcatcaat tctgtactgt tcattttatt ctctgtgata gctttgatta atggtgctga
     1261 aaataccgat tttcatttta aattgcaaat acttgtcaga aacacgcttg atctatgtct
     1321 aatatacttc attttttaac ttaggtatga cgatgagagc acttatccac acttttcgta
     1381 gcacgacttt agacatcacg attttacgtc ttagcgtatg tctaatcttc tttactttcg
     1441 gtatgacaaa atggtttgat tttgaggtgg aaatccttaa gccgatgatc agtccgactt
     1501 ggttgaattt tctctatgag tggctaggct atcacggcac aagttatttc ttaggtgtgg
     1561 ttgaagggat tgcttatata gcacttgccg ttggctattg gaaacctagg tggggaatta
     1621 tcggttcaat gacagtatta ctggtttcat ctgttacact tagcctgttg ctacagcttg
     1681 gttttaacag ctttattttc aaggatattc tacttatcgg cgcaggatta gtattattaa
     1741 aatacgatct aaaccgcctt tacccccacg aatagctcac gttgtttttt gatctgcccc
     1801 aaaagttgga ctaagccgtg agtaatacag aactcactcc ttgaattaaa tgaaaagatg
     1861 gtcatattgt acaatccact tatcaacgta tttttatccg ccataaaaag taggtcgggc
     1921 atctatgccc gacaattttc cgatatattc aaccattgta ccgataatat aaattttcgg
     1981 gcatttatgc ccgacttgga gacaaaatgc aaacgaaatt aaataccgaa atcaccattg
     2041 aaaaactgtg cgaaggtttt caatataacg aacttgaagg caaaggctta tttggtttaa
     2101 acggtaaatt aactattcag cctgaatatc aacgtaacta tatttatgcg gacggtaaaa
     2161 aagatgtggc ggttattcac tctattttga aaggctatcc acttggtttg atttatttta
     2221 atcaaacaga tgatgataaa tttgaagtgt tagacggtca gcaacgcatt actagtatcg
     2281 ggcgatttgt tacgaataaa tttgccattt tagatcaaaa taatatccct tgctattttt
     2341 ccagtttgcc tgaagataaa cgccaattca ttttaaatac cacgttatta atttatgaat
     2401 gcaaaggcac tgaaagcgaa attaaagagt ggtttaaaac cattaatatc gcaggcgtac
     2461 cactcaatga gcaagaatta ttaaatgctg tttattcagg ctcatttgtc acccttgcta
     2521 aagcagaatt tagcaattct caaaatgcca atgtgcaaaa atggtcggca tttgtgaaag
     2581 gttcagttat tcgccaagat tttttggctt gtgcgttaaa ttgggtaagc aagggaaata
     2641 tcagtgatta tatgagtcgc catcgttttg atgagaatat cgctgaattg aaaacttact
     2701 ttactaccgt gattgattgg gtaaatacca cattctttga tgctaaaaaa gagatgtgtg
     2761 gtttagaatg gggcagactt tatgaaaatt atggcaagca agcttatgat ttaagcaaaa
     2821 ttaatgctga aattgaccgc ttttatgatg acccttgtgt gaaaaaccat aaaggtattt
     2881 gggaatatgt attgggcgga atgacagata aacgtttgct agatattcgg atttttgacg
     2941 acaaaacaaa aaaagatacc tatagcaagc aaaccgaaaa agccaaagta gccaatcact
     3001 ctaactgccc attgtgtgct gtgagcgata ataacaataa aaccaaaatt tggacattaa
     3061 aagagatgga agccgaccac gttagtgcct ggagtaaagg agggttaact ggagcagaaa
     3121 actgcgaaat gttgtgtatc agccataatc gagcaaaagg aaacaaatga taattttaca
     3181 aacggttagt ttcaacg
//
LOCUS       DANJWC010000199         3164 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.200, whole genome shotgun sequence.
ACCESSION   DANJWC010000199 DANJWC010000000
VERSION     DANJWC010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.200"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(70..2968)
                     /locus_tag="PWX02_002249"
     rRNA            complement(70..2968)
                     /locus_tag="PWX02_002249"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          718 a          919 c          611 g          916 t
ORIGIN      
        1 attgatttct tctctagttt cgttcttctt ctcaatacaa aaacaagcct aaaaacattt
       61 gagcgttgta tagttaagcc tctcgggcaa ttagtactgg ttagctcaat gtctcacaac
      121 acttacacac ccagcctatc tacgtcgtag tctccaacaa cccttacaca cttatagtgt
      181 gggagaactc atctcgaggc aagtttcgtg cttagatgct ttcagcactt atctcttccg
      241 catgtagcta cccagcaatg cctctggcga gacaactgga acaccagtga tgcgtccact
      301 ccggtcctct cgtactagga gcagcccctc tcaattctcc aacgcccacg gcagataggg
      361 accgaactgt ctcacgacgt tctaaaccca gctcgcgtac cactttaaat ggcgaacagc
      421 catacccttg ggacctactt cagccccagg atgtgatgag ccgacatcga ggtgccaaac
      481 accgccgtcg atatgaactc ttgggcggta tcagcctgtt atccccggag taccttttat
      541 ccgttgagcg atggcccttc cattcagaac caccggatca ctatgacctg ctttcgcacc
      601 tgctcgactt gtctgtctcg cagttaagct tgcttatacc attgcactaa cctcacgatg
      661 tccgaccgtg attagcaaac cttcgtgctc ctccgttact ctttgggagg agaccgcccc
      721 agtcaaacta cccaccagac actgtccgag accgcgttcc gcaatcttcg ttagaacatc
      781 aaacgttaaa gggtggtatt tcaaggacgc ctccacaatc actggcgtga ctgcttcaaa
      841 ggctcccacc tatcctacac atcaaaattc aatgttcagt gtcaagctat agtaaaggtt
      901 cacggggtct ttccgtctag ccgcgggtac accgcatctt cacggcgatt tcaatttcac
      961 tgagtctcgg gtggagacag cctggccatc attatgccat tcgtgcaggt cggaacttac
     1021 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct
     1081 tcgatcagga gcttctcttg cgataacacc atcaattaac cttccagcac cgggcaggca
     1141 tcacacccta tacgtccact ttcgtgtttg cagagtgctg tgtttttaat aaacagttgc
     1201 agccagctgg tatcttcgac cggttcaacc ttcatccgca agggactaca atctacgccg
     1261 gcgcaccttc tcccgaagtt acggtgctat tttgcctagt tccttcaccc gagttctctc
     1321 aagcgcctga gtattctcta cctgaccacc tgtgtcggtt tatagtacgg tttataataa
     1381 cctgaagctt agtggctttt cctggaagcg tggtatcggt tacttcatct ccgtagagac
     1441 tcgtcatcac ttctcggtgt taaggaagac cggatttgcc taatcttccc acctaccggc
     1501 ttaaacagac atccaacagt ctgctaacct aaccttctcc gtccccacat cgcagttatt
     1561 acaagtacgg gaatattaac ccgtttccca tcgactacgc ttttcagcct cgccttaggg
     1621 gccgactcac cctgccccga ttaacgttgg acaggaaccc ttggtcttcc ggcgaacgag
     1681 tttttcactc gttttatcgt tacttatgtc agcattcgca cttgtgatat ctccagcaga
     1741 cttctcaatc caccttcatc gacttacaca acgctcccct acccaacaga ctttcgtctg
     1801 atgccgcagc ttcggtgcta tatttgagcc ccgttacatc ttccgcgcag gccgactcga
     1861 ctagtgagct attacgcttt ctttaaatgg tggctgcttc taagccaaca tcctagctgt
     1921 ctaagccttc ccacttcgtt tcccacttaa tatagacttt gggaccttag ctggcggtct
     1981 gggttgtttc cctctccacg atggacgtta gcacccaccg tgtgtctcct gagtatcact
     2041 cttcggtatt cgcagtttgc atcgggttgg taatccggga tggaccccta gccgaaacag
     2101 tgctctaccc ccgaaggtgt ccgctcaagg ctctacctaa atagatttcg gggagaacca
     2161 gctatctccc ggtttgattg gcctttcacc cccagccaca agtcatccgc taatttttca
     2221 acattagtcg gttcggtcct ccagttagtg ttacccaacc ttcaacctgc ccatggctag
     2281 atcaccgggt ttcgggtcta taccttgcaa ctacacgccc agttaagact cggtttccct
     2341 acggctccct tattcagtta accttgctac aaaatataag tcgctgaccc attatacaaa
     2401 aggtacgcag tcacaagatt actcttgctc ccactgcttg tacgcacaag gtttcaggtt
     2461 ctatttcact cccctcgccg gggttctttt cgcctttcct tcacagtact ggttcactat
     2521 cggtcaatca ggagtattta gccttggagg atggtccccc catcttcaaa caggatatca
     2581 cgtgtcccgc cctacttgtt gttagcctag taccacaatg gagttttaag gtacgggatt
     2641 atcaccctct acgattgtcc ttcccagaac attcccctaa ctttactgct atcactaact
     2701 ggctctttcg cgttcgctcg ccgctactaa cgaaatctcg gttgatttct tttcctcggg
     2761 gtacttagat gtttcagttc tcccggtttg cctcacacag ctatgtattc actgggtgat
     2821 agtaggttct tcacctactg ggtttcccca ttcggacatc ttggattaaa cgcctcttat
     2881 cgactcatcc aagcttttcg cagattagca cgtccttctt cgcctctgat tgccaaggca
     2941 tccaccttgt gcgcttagtc acttaactat acaacctcaa atgttttcta aaacttctca
     3001 aaaataaaag taaaaaaaca atgaagtcag ttttacaact aaacacttga ctgcttttgt
     3061 tcagtcaaga ttttttctac tcagactttc aatttattcg ttatcactta tgtataatta
     3121 cttatgcgta acgaacttga aatatctctc agtttttcag cttg
//
LOCUS       DANJWC010000200         3129 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.201, whole genome shotgun sequence.
ACCESSION   DANJWC010000200 DANJWC010000000
VERSION     DANJWC010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.201"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            177..455
                     /gene="ftsB"
                     /locus_tag="PWX02_002250"
     CDS             177..455
                     /gene="ftsB"
                     /locus_tag="PWX02_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HDL1730219.1"
                     /translation="MRLLITFFALLLGFFQYSFWFGKNGWSDYQEVQEEIALLKVENT
                     KLTSRNKLIEAEIYDLKNGVDALEERARLKREMVKENEIFYRIVPKNK"
     gene            473..1156
                     /gene="ispD"
                     /locus_tag="PWX02_002251"
     CDS             473..1156
                     /gene="ispD"
                     /locus_tag="PWX02_002251"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDL1730220.1"
                     /translation="MKRKIIAVIPASGVGSRMQAEKPKQYLQLKGKTILEHTVSIFLE
                     HPHVEKVIVAVARKDPFYSTLELLKSPKIQLVFGGETRADSVLNALQEINTENAWALV
                     HDAARPCLKRSDLDKLLQINDPQGGILAIPAVDTMKRANGTKIAHTEDRSTLWHALTP
                     QFFPAQALKQALSSAFANKLQVTDEASAMELAGYQPQLVSGRSDNIKITRPEDLALAE
                     FYLTHNLEN"
     gene            1158..1637
                     /gene="ispF"
                     /locus_tag="PWX02_002252"
     CDS             1158..1637
                     /gene="ispF"
                     /locus_tag="PWX02_002252"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDL1730221.1"
                     /translation="MIRIGHGFDVHAFGEDRPLMIAGVEVPYHTGFIAHSDGDVALHA
                     LTDALLGAAALGDIGKLFPDTDMQYKNADSRGLLIEAYRQVQEQGYQVGNVDVTIIAQ
                     APKMRPHIDKMRQVIADDLQCDISQVNVKATTTEKLGFTGRGEGIACEAVALLLKRA"
     gene            complement(1752..2588)
                     /locus_tag="PWX02_002253"
     CDS             complement(1752..2588)
                     /locus_tag="PWX02_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL1730222.1"
                     /translation="MMKSLCKFPSIFNRLLLVCLALFSLNATAVTYPEPPNPFYYITD
                     YTKNTLSQQEWRTLEDALIANRAKTSSQIAVVIIPSTEGEAVSTYAHTLFNKWGIGRS
                     KNNENNGILLLIAKNDRKLFIATGRGLEGALPDAIASSIIRNDITPFFKQEQYAAGIA
                     RGLSAIMAAINGEYAPYASYGEEMQEFDDIDGLLFFLFAGIVIFLIFRPRGNSTYISP
                     SSMDQLGQILRESQRRGGGFGGGLGGGFGGGFGGGFGGSRGSGDSFGGGSSGGGGAGG
                     SW"
     gene            complement(2585..3043)
                     /locus_tag="PWX02_002254"
     CDS             complement(2585..3043)
                     /locus_tag="PWX02_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL1730223.1"
                     /translation="MGLFSFLSNKLPIDTQRIEQAIADLEQKTSAELRVVVERKAKIG
                     KTDNAAMLRASELFDELKMRETAEHNGVLIYLSFKPHYAAIIGDEGIHQKVDNDFWQK
                     IYATMCLDCQQGDYTQAICDAIKMVEAPLAHHFPYQADDKNELSNEVVVK"
BASE COUNT          945 a          640 c          655 g          889 t
ORIGIN      
        1 tttaaaaaaa ccgctttaaa atacgcattt gtttgtttta tatcaatttt tcgaatacat
       61 ttagcactat ccatcgcctg tttaagtaac aagcggtaag atttttagaa ttttttgcaa
      121 attcaaggta aaatcaaccc gcttagttca ttactatttc atataagaga aaataaatgc
      181 gtttattaat tacttttttt gccctattac tcggcttttt ccaatatagc ttttggtttg
      241 gaaaaaatgg ttggagtgat tatcaagaag tgcaagaaga aattgcttta ttaaaagtag
      301 aaaacacaaa gcttacttca cgcaataaac taattgaagc cgaaatttac gatttaaaga
      361 acggcgtaga tgcacttgaa gagagagcaa gacttaaacg agaaatggta aaagagaacg
      421 agattttcta ccgaattgta ccaaaaaaca aataatacat aattgatata ttatgaagcg
      481 aaaaattatt gcagtaattc cggcgtcagg agttggtagc cggatgcaag cagaaaaacc
      541 aaaacaatat ttacaattaa agggtaagac tatcttagag catacagtgt ctatttttct
      601 tgaacaccct catgttgaaa aagtgattgt agcggtggca agaaaagacc ctttttattc
      661 cacattggaa ctcctaaaat cccctaagat ccaacttgtt tttggaggag aaacgagagc
      721 agattcagtg ctaaatgcct tacaggaaat caacactgaa aatgcttggg cattagttca
      781 cgatgcagct cgcccttgcc tcaagcggtc agatttagat aaacttttgc aaattaacga
      841 tccgcaaggt ggcattctcg ccattccagc ggtagatacg atgaaaagag caaacggcac
      901 gaaaatcgca cacacagaag atcgctcaac cttatggcac gctttaaccc cacaattttt
      961 tcctgctcaa gcattaaaac aagcattatc ctctgctttt gccaataagc ttcaggtaac
     1021 agacgaagca tctgcaatgg agcttgcagg ctaccagcca caattggttt caggtagaag
     1081 tgataatatc aaaatcactc gccctgaaga tttagcctta gcggaatttt accttacaca
     1141 taatttggag aactaaaatg atacgtattg gacacggttt tgatgttcac gcttttggcg
     1201 aagatagacc actgatgatt gctggggttg aagtgcctta tcatacaggc ttcattgcgc
     1261 atagtgacgg agatgtagcg ctgcacgctt taacagatgc gttgcttggc gcagcagctt
     1321 taggtgatat tggcaaatta ttccctgata ctgatatgca atacaaaaat gccgatagtc
     1381 gtggtttgtt gattgaagct taccgacaag tgcaagaaca gggctatcaa gtcggtaacg
     1441 tcgatgtaac cattattgct caagctccga agatgcgacc gcatattgat aaaatgagac
     1501 aggtgatcgc agacgatcta caatgcgata tttcacaagt gaatgtaaaa gccactacaa
     1561 cagaaaaatt agggtttacc gggcgaggtg aaggcatagc ttgtgaagcc gtagcattgt
     1621 tactaaaaag ggcatagctt agagaagtga ggaagcggtc ttattttcat tagcgttttg
     1681 tgcgtttagc gaaaaatacg caattttttt gagaaatatg accgcttgta ttgttttaaa
     1741 ttgggcggaa actaccagct tcctccagcg cctccaccgc ctgatgaacc accaccgaag
     1801 ctgtcgccac tgcctctgct tccgccaaaa ccaccaccga agcctccgcc aaatccacct
     1861 ccgagaccac cgccaaagcc cccacctctg cgttggcttt ctcttagtat ttgtcctagt
     1921 tgatccattg agctgggact aatataggta ctgtttcctc gtgggcggaa aattaggaaa
     1981 atcacaatgc cggcaaacaa gaaaaagagt aagccgtcta tgtcatcaaa ttcttgcatt
     2041 tcttcgccat aacttgcata aggggcatat tccccgttaa tggctgccat aatggcggat
     2101 aagccacggg cgataccggc tgcatattgc tcttgtttga agaatggcgt gatgtcattg
     2161 cggataatgc tcgatgcaat cgcatcaggt aacgctcctt caagcccacg tccggtggca
     2221 ataaagagtt tgcgatcatt tttggcaatt aataacaata taccattgtt ctcgttattt
     2281 ttgctccgtc cgattcccca tttgttaaac agggtatgag cataggttga aactgcctcg
     2341 ccttccgtgc ttggaataat caccacggca atttgcgaac ttgtcttggc acggttagca
     2401 attaaggcat cttctaatgt ccgccattct tgttggctca aggtgttttt ggtgtagtcc
     2461 gtaatataat aaaacggatt aggcggctct gggtaggtca ccgctgtggc atttaggcta
     2521 aacagagcca agcacaccaa taacaagcgg ttgaaaatag atggaaattt gcaaagggat
     2581 ttcatcattt gactaccacc tcgtttgaaa gctcgttttt atcatcagcc tgataaggga
     2641 aatggtgagc gagcggagct tccaccattt tgattgcatc acaaatggct tgggtgtaat
     2701 cgccttgttg gcagtccaaa cacattgtgg catagatttt ttgccaaaaa tcgttatcga
     2761 ctttttgatg aatgccttca tcgccaataa tcgcagcgta gtgaggcttg aatgaaaggt
     2821 agatcaacac accgttatgt tcagccgttt cacgcatttt taattcatcg aaaagctcac
     2881 ttgctcgtaa catagcggcg ttgtcggttt tgccgatttt ggcttttcgt tccaccacca
     2941 cacgcaattc ggcagaggtt ttttgctcta aatcggcaat tgcttgttca atgcgttgcg
     3001 tatctatcgg taatttgttg gagagaaaag aaaataatcc catttgttcc ccttaaaaag
     3061 aaagcaataa gcaccgaatt tgaaagagct tattgctttt aatatgaata aattaaaact
     3121 aaaattagt
//
LOCUS       DANJWC010000201         3119 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.202, whole genome shotgun sequence.
ACCESSION   DANJWC010000201 DANJWC010000000
VERSION     DANJWC010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.202"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..542
                     /locus_tag="PWX02_002255"
     CDS             <1..542
                     /locus_tag="PWX02_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL1730224.1"
                     /translation="IATDGATLDVGQAIYNRLIDFEKGTTNLTPSLAERWEISEDGKI
                     YTFFLCQNVQFQSNKSFTPSRYFNADDVIFSINRQFDKTKPYHKLPGGNYEFFYGMNM
                     QNIIKNVTKVDDYTVKIELSEPNAPFLANLAMDFMAIFSAEYANSGYAGKIRFRTYRH
                     RCIPVLWLPKGRCGKIYSI"
     gene            433..780
                     /locus_tag="PWX02_002256"
     CDS             433..780
                     /locus_tag="PWX02_002256"
                     /inference="COORDINATES: protein motif:HMM:NF012706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730225.1"
                     /translation="MQIAGTPEKLDLEPIGTGAFQFYGYQKDAAVRFTAFEHYWEGKA
                     KIDRLVFSITPDASVRLAKLEKNECQAMPYPNPSDLPALKANKDIQLFEKSGLNIGIL
                     VSPLLITLKFVKH"
     gene            777..1448
                     /locus_tag="PWX02_002257"
     CDS             777..1448
                     /locus_tag="PWX02_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL1730226.1"
                     /translation="MNYAVNKTEILTSVYQGSATPAKNPMPSTIWGYNNEVAGYEFNP
                     EKAKSLLKEAGLENGFETEIWAMPVSRPYNPNARRMAEMIQEDWKAIGVEAKIVSYEW
                     GEYLKRMGNGEHATGMIGWTGDNGDPDNFLNALLSCNAVEQGSNYAGFCNPEFDRLIN
                     EAATISDQNKRSDLYKKAKFIFNEQAPWLPIAHSTVYFPVRKEVKGYVVSPFIAHNFY
                     GVELE"
     gene            1508..1957
                     /locus_tag="PWX02_002258"
     CDS             1508..1957
                     /locus_tag="PWX02_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudoazurin"
                     /protein_id="HDL1730227.1"
                     /translation="MLAKLKWNQLACVTLAFCSASVFSAHHEIKMLDNNAEGSMVFEP
                     GYVKAEVGDTVTFIPSHKGHWVESRSIPEGAEKFLSKEDEEFTITLTHEGVYVYYCPP
                     HRTMNMAGIIQVGQALNLEATQKEVEKIEKRTKENKGRLFEYLEQVK"
     gene            2058..2579
                     /locus_tag="PWX02_002259"
     CDS             2058..2579
                     /locus_tag="PWX02_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Type II secretory pathway, pseudopilin PulG"
                     /protein_id="HDL1730228.1"
                     /translation="MYKAITLIEILTTITIMVIAVYFISPVIFTLQDRIALHSEIENI
                     QSFIQQIQTKARYTKRNYTLTLSQRNNQWCMIAVIKPINTSKQIICDCLNKHFCDLGK
                     EYHLYTPTHQQIVLKNKSLYPKSFINVDGVSGQLESKCIKLSLNKENEILQFEQLGRV
                     YVAAKNKRSTCKD"
     gene            2611..>3119
                     /locus_tag="PWX02_002260"
     CDS             2611..>3119
                     /locus_tag="PWX02_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730229.1"
                     /translation="MASALGAFLLLLVASSYADFYYTQTKQKEQLYLQAEAHQLLNYF
                     QQHFQHIGYQGSHRTDSNFDLFQQAGKSLNIVHKNCIIGFYDLNQDGCLGKRKTKTAA
                     CKLGTVNNTREVLKEIVGFKVENNEIYTFSSQLDNCVKEQCQTLLENCDGNWSKFTEA
                     NNFKTKKLD"
BASE COUNT         1062 a          524 c          625 g          908 t
ORIGIN      
        1 agattgcaac agatggagct actcttgatg tcgggcaagc aatctataat cgcctgattg
       61 attttgaaaa aggtacaact aacctcactc cttctctggc agagcgatgg gaaatcagtg
      121 aagacggaaa aatttatact tttttcttgt gtcagaatgt gcagttccag agtaataaaa
      181 gttttacccc tagtcgttat tttaatgctg atgatgttat tttttcgatt aaccgtcagt
      241 ttgataagac gaagccatat cacaaattgc ccggcggtaa ctatgagttt ttctacggaa
      301 tgaatatgca aaacatcatc aaaaatgtaa caaaagtaga tgattacacg gtaaaaatcg
      361 aattatctga accaaatgcc ccctttcttg ctaatttagc tatggatttt atggcgatct
      421 tttctgcgga atatgcaaat agcgggtacg ccggaaaaat tagatttaga acctataggc
      481 acaggtgcat tccagttcta tggttaccaa aaggacgctg cggtaagatt tacagcattt
      541 gaacactatt gggaaggaaa agcgaagata gatcgccttg tatttagtat tacgcccgat
      601 gcctcagtac gtttagctaa attggagaag aacgaatgcc aagcgatgcc ttatccgaac
      661 ccgtcagatt tgcctgcttt aaaagcgaat aaagatattc aattatttga aaagtcaggc
      721 ctaaatatcg gaatactcgt aagccccctt ttgataaccc taaagttcgt caagcattga
      781 attatgcggt gaataaaact gaaattttaa cttccgttta ccaaggctct gcaacgccag
      841 ctaaaaaccc aatgccttct acgatttggg gatataacaa cgaagtagcc ggttatgaat
      901 ttaatccgga aaaggcgaag agcttgctta aagaggccgg attagaaaat ggctttgaaa
      961 cagaaatttg ggcaatgcct gtttctcgcc cttacaatcc aaatgcccgt agaatggctg
     1021 aaatgattca agaggattgg aaagcaatag gggtagaggc aaagatagtg agctatgaat
     1081 ggggagaata tcttaaacga atgggaaacg gcgagcacgc taccggaatg attggttgga
     1141 ccggagataa cggcgatcca gataatttcc tcaatgccct actcagttgt aatgcggtag
     1201 aacaaggctc aaactatgca gggttttgta atccagagtt tgaccgttta attaatgagg
     1261 ctgcgactat cagtgatcaa aacaagcggt cagatttata taaaaaagcg aaattcatct
     1321 ttaatgagca agcaccttgg ttaccgattg cacattcaac ggtgtatttt ccggtaagga
     1381 aagaggtaaa aggctatgtt gtcagcccgt ttattgctca taatttttat ggtgttgagc
     1441 tggaataacc taaggtataa tgaattgaac tcattttgtg cctcataaat ttaggagcaa
     1501 taaatatatg ttagcgaaac ttaagtggaa tcaattagct tgtgtaacat tggcattttg
     1561 ttcagcctca gtattttcag cccatcacga aatcaaaatg ttagataaca atgcggaagg
     1621 atcaatggta tttgagcccg gttatgtaaa agcagaggtg ggcgatacgg taacatttat
     1681 tccgtctcat aaagggcatt gggttgaaag ccgctcgatt ccggaaggtg cggagaagtt
     1741 cttatctaaa gaagatgagg aatttactat tacccttact cacgaaggtg tttatgttta
     1801 ctattgtccg ccacaccgaa ccatgaatat ggccggaatt atccaagtag gacaagctct
     1861 caatcttgaa gccacacaaa aagaagtgga aaaaattgaa aaacgcacga aagaaaataa
     1921 aggacgttta tttgaatatt tagagcaagt taagtagaag tgatttaatt ttgcgatctg
     1981 catcgaaaaa tcaatattta atagtttaca aatgatgctt aaattctaag ataccctcta
     2041 tcaatgaggg tatttttatg tataaagcca tcacattaat tgaaatacta accactatca
     2101 ctattatggt gatcgctgtt tattttatct cgccggttat ttttacactg caagatcgaa
     2161 ttgcacttca tagtgaaatt gaaaatatcc aatcttttat tcagcaaatc caaactaaag
     2221 cacgttatac caaacgtaat tataccttaa ccctttctca gcggaataat cagtggtgta
     2281 tgattgcggt aattaagcca atcaatacta gcaaacaaat tatttgtgat tgtttgaata
     2341 aacatttttg tgatttagga aaagaatatc atctttatac gccgactcat cagcagattg
     2401 tattaaaaaa taaaagttta tatccgaaat cctttattaa tgttgatggg gtaagtggtc
     2461 aattagaatc aaaatgtatt aagctcagtt taaataaaga aaatgaaatt ttgcaatttg
     2521 aacaattagg acgggtttat gtggcagcaa aaaataaacg tagcacctgc aaagattagt
     2581 ttatctgctt ttagcctaat tgaagtatta atggctagtg cattaggggc gtttttatta
     2641 ttgcttgttg cttcaagtta tgccgatttc tattacacac aaaccaaaca aaaagaacag
     2701 ctctacttgc aagcagaagc tcaccagtta ctaaactact tccaacagca ttttcaacac
     2761 attggctatc aaggctctca cagaacagac agtaattttg atttatttca gcaagcaggg
     2821 aaaagcctga atattgtgca taaaaattgc attattggat tttacgattt aaaccaagac
     2881 ggctgtttgg gaaaacgtaa gacaaaaacg gccgcttgta agttaggaac agtgaataat
     2941 accagagagg tgttaaaaga aattgttggc tttaaagtag aaaataatga aatttatact
     3001 ttttcatcgc aattagataa ttgtgtgaaa gaacaatgcc aaacattatt ggaaaattgc
     3061 gatggaaatt ggagtaaatt taccgaagca aataatttca aaactaagaa actggattt
//
LOCUS       DANJWC010000202         3106 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.203, whole genome shotgun sequence.
ACCESSION   DANJWC010000202 DANJWC010000000
VERSION     DANJWC010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.203"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..1228
                     /gene="purB"
                     /locus_tag="PWX02_002261"
     CDS             <1..1228
                     /gene="purB"
                     /locus_tag="PWX02_002261"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDL1730230.1"
                     /translation="ASHTQINEVPVFSEKANDYLNQIVADFSLEDANRIKTIERTTNH
                     DVKAVEYFLKEKCEALPELQAVNEFIHFACTSEDINNTSHALMLKTAREEVLLPEWKK
                     VIDAVAELAKCYQHIPLLSRTHGQPASPTTMGKEMANVAYRLQRQYKQLENLEILAKI
                     NGAVGNYNAHLSAYPEIDWHTFSQEFVESLGVTWNPYTTQIEPHDYIAEFFDCVARFN
                     TILIDFDRDMWGYIALNHFKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGLANAVMAH
                     LGQKLPISRWQRDLTDSTVLRNLGVGLGYALIAYASTLKGISKLEVNEQHLRDELNQN
                     WEVLAEPIQTVMRRYGIEKPYEKLKELTRGKRVDETAMREFIDKLEIPENEKNRLKEM
                     TPASYIGYAVELVDKL"
     gene            1295..2056
                     /locus_tag="PWX02_002262"
     CDS             1295..2056
                     /locus_tag="PWX02_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HDL1730231.1"
                     /translation="MKQANRHKKIIELVNQLGYVSTEQLVAELNVSPQTIRRDLNEMA
                     ENNLIRRHHGGAAAPSNTENSDYTHRKQFFSHEKNAIAQQVAKLIPNGASLFLDIGTT
                     SEAVALALLSHKNLKVVTNNLNAAHILMQNEDCQITVAGGGLRSDGGLIGEETVRFIN
                     QFRLDFGILGISAVDMDGSMLDYDYHEVQVKRALMECSRQVVLVTDHSKFTRTAIARL
                     GNVKEVNYLFTDQDLPLELQTHLSQSDVIVKICNE"
     gene            2049..2924
                     /gene="ubiA"
                     /locus_tag="PWX02_002263"
     CDS             2049..2924
                     /gene="ubiA"
                     /locus_tag="PWX02_002263"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HDL1730232.1"
                     /translation="MNNETLFSKQKWIAYAQLMRFDKPIGTLLLLHPTLWALFIAAEG
                     MPPVAILAIFTLGVIVMRAAGCVINDYADREIDGHVKRTSQRPLATGRVTTTEAKVIF
                     AILLLFAFLLDLMLNRYAFLLSFVAVFLAVIYPFMKRFTHLPQVVLGMAFGWSIPMAF
                     GAVTETLPLECWLLFFANLAWTVAYDTQYAMVDRDDDLRIGVKSTAILFAQYDNKIIA
                     LLQFITLILLGLLGVVKDYHIGYFVVLALSATLFIYQCWLTKKRERSECFKAFLNNHY
                     FGLGVFVAILVGIYF"
BASE COUNT          923 a          584 c          680 g          919 t
ORIGIN      
        1 ggcatcgcat acacaaatta atgaagttcc agtgttttct gaaaaagcaa acgattacct
       61 aaaccaaatt gtggctgatt tttcgttaga agatgccaat cggattaaaa cgattgaacg
      121 taccaccaac cacgatgtaa aagcggtgga gtatttccta aaagagaaat gtgaagcgtt
      181 gcctgaatta caagcggtga atgaatttat tcattttgct tgtacttctg aagatatcaa
      241 taatacttcg cacgctttaa tgcttaaaac cgctcgtgaa gaagtattat tacctgaatg
      301 gaaaaaagtg attgatgctg tagcagagct ggcgaaatgt taccaacata ttccgttgct
      361 ttctcgcaca cacggtcagc cggcaagccc gaccacaatg ggtaaagaga tggcaaatgt
      421 ggcatatcgt ttacaacgcc aatataaaca gttagaaaat ttagaaattc tcgctaaaat
      481 caatggagca gtgggcaact ataacgcaca tttatcggca tatccggaga ttgattggca
      541 cactttcagc caagaatttg tggaatcgtt aggtgtgacg tggaacccat acaccaccca
      601 aattgaaccg cacgattata ttgcggagtt ctttgattgc gtggctcgtt ttaatacgat
      661 tttaattgat tttgatcgtg atatgtgggg ctacattgcc ttaaatcact tcaaacaacg
      721 cactattgcc ggtgaaatcg gctctagcac aatgccacat aaagtgaacc cgattgattt
      781 tgaaaactcc gaaggcaatc ttggcttggc aaatgcggta atggctcatt tggggcaaaa
      841 attgccgatt tctcgctggc agcgtgactt aactgactcc accgtgttgc gtaatttagg
      901 tgtaggttta ggttatgcgt taattgctta tgcgtccacc ttaaaaggta ttagcaaatt
      961 agaagtgaat gaacaacact tgcgtgatga actcaaccaa aactgggaag tattggcaga
     1021 gccaattcaa actgttatgc gtcgttatgg cattgaaaaa ccgtatgaaa aacttaaaga
     1081 gctgactcga ggcaaacggg ttgatgaaac cgcaatgcgt gagtttatcg ataagttgga
     1141 aattccggaa aatgaaaaaa atcgtttgaa agagatgaca ccggcaagct atatcggcta
     1201 tgcggttgaa cttgttgata aattgtaaaa tttaactgaa aaaagaccgc ttgcaagcgg
     1261 tctttttctg caaattattt gcaaaagagg aaatatgaaa caggcaaaca gacacaaaaa
     1321 gatcatcgag ctggttaatc agcttggcta tgtgagtacc gagcaattag tggctgaatt
     1381 gaatgtgagt ccgcaaacca ttcgccgtga tctgaatgaa atggcagaaa ataatctgat
     1441 tcgccgtcat cacggtgggg cagccgctcc ttccaatacg gagaatagtg attacaccca
     1501 ccgcaagcaa tttttctcac acgagaaaaa tgcgattgcc caacaggtgg caaaactgat
     1561 tccaaatgga gcatcattat ttttagatat tggcacgact tcagaagcag tggcattagc
     1621 cttactttcg cataaaaatt taaaagtagt taccaataat ttaaatgcgg ctcatattct
     1681 gatgcaaaat gaagattgcc aaattacggt agcaggcgga gggctaagaa gtgatggcgg
     1741 gctaatcgga gaagaaacgg ttcgctttat taaccaattc cgtttggact ttgggatttt
     1801 aggcattagt gcggtggata tggacggctc tatgctcgat tacgattacc acgaagtgca
     1861 ggtaaagcgt gcattaatgg aatgttcccg acaagtggtt ttagtgaccg atcattcaaa
     1921 gtttacccgt acagctattg cacgtttagg aaatgttaaa gaagtaaatt atttgtttac
     1981 agatcaagat ttaccgttag aattacaaac tcatttaagc caatcggacg ttatcgtcaa
     2041 aatttgtaat gaataacgaa actctttttt ctaaacaaaa atggattgcc tacgcccagt
     2101 taatgcgttt cgataaaccc atcggcacgt tattattatt acaccccaca ctatgggcgt
     2161 tatttattgc cgcagaggga atgccgcctg ttgcgatttt agccattttt acccttgggg
     2221 taatcgttat gcgtgcggca ggttgtgtga ttaatgatta tgccgataga gaaattgacg
     2281 gacacgttaa acgcacctct caacgcccat tagctaccgg cagagtgacc accactgagg
     2341 caaaagtcat ttttgccatc ttgctgttgt tcgcttttct gctcgattta atgctaaacc
     2401 gctatgcgtt tctgctttcc tttgtggcag tttttctggc ggtgatttat ccctttatga
     2461 agcgttttac ccacttgcca caagtagtgc ttggaatggc attcggttgg tcgattccga
     2521 tggcatttgg tgcggtaacg gaaactcttc cacttgaatg ctggctgctg ttttttgcca
     2581 atctagcgtg gacagtcgcc tacgacaccc aatatgcaat ggttgatcgt gacgatgatt
     2641 tacgaattgg agttaaatcc actgcaattt tattcgctca atacgataat aaaattattg
     2701 cgttattaca gtttatcacc ttgattttat tgggattatt gggcgtggta aaagattatc
     2761 acatcggcta ttttgtcgta ttagccttaa gtgccacgct gtttatctac caatgttggc
     2821 tgaccaaaaa aagagaacgt agcgaatgct ttaaagcgtt tctaaataac cactatttcg
     2881 gcttaggtgt gttcgtggcg attttagtcg ggatttattt ctgatgacgg taggggctaa
     2941 aaatgtttag ctcctatttt ttatctatgg tttttatcta caagcggtga aatttatcgg
     3001 gaaatttgta aaatatatcc agttgtagtt aattatattc ttgcttcatt agtcaatcgg
     3061 tttgtaaaat atcccatttt gtagtagaca ttcaaaaaag ggacat
//
LOCUS       DANJWC010000203         2984 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.204, whole genome shotgun sequence.
ACCESSION   DANJWC010000203 DANJWC010000000
VERSION     DANJWC010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.204"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            1..1000
                     /locus_tag="PWX02_002264"
                     /pseudo
     CDS             1..1000
                     /locus_tag="PWX02_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            1055..2905
                     /locus_tag="PWX02_002265"
     CDS             1055..2905
                     /locus_tag="PWX02_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL1730233.1"
                     /translation="MQVKKFSQKYINWVLKLGRLKASLLGFFMVALSAILIQSSLTYL
                     FIGRVVVVDILRSIAFGLISAPFVLYFFTLLVEKLEISRLKLEKAVRDLEELREQDAY
                     LNAKLEKNSRDKSILMATISHELRTPLNGIIGLSQILLDDKTTEKQQEYLKTINMSAT
                     ALAHIFGDIIDLEKIDSSRIELVRKEVEFEQLIHDIANFGHFMAGNKQINFEVKYPPD
                     LPKYIYIDHARLSQILWNLISNAVKFTPASGRILLEIYQLDKEQFSFAVTDSGIGIPE
                     KDQRKIFDMFYQAENSKEKKAQGSGIGLSVSKRIAKLMGGDLVVASQENQGSTFTLTV
                     QAESTENKPTFAIQQHSLKVLLVEDIEMNVVVARAMLEKIGCEVDVAMSGEETYQHFA
                     QDSYDLILLDIQLPDTTGFEIAQQLRLQYENEEIDYLPLLVALTANVIQSRQEYQAQG
                     MDDVLHKPLSLDELMRCLNQHFGDDFLQKNEEKQPLVERGSENLPYSITILNELIGVM
                     GKENVLANMDLFADLMPTYLANLQRYLSDWQTTNSVESRKLTADEAHKIKGALASVGL
                     HQLQQTAQLAQSDNGEQWEQNIAHWVEMICSKWQMDLQLAKEYVRSKGDI"
BASE COUNT          952 a          506 c          685 g          841 t
ORIGIN      
        1 atgccgggcg atgcaaataa aattatctct aacggcacca gtgccggagg agcattatct
       61 actttgttag gagcttcagc tgatcattac gactatgagc cttatttgaa agaggcgggg
      121 gcattgaatg ccagtgacaa aatttttgca gtttctgcct attgcccaat taccaacctt
      181 gaaaatgcgg atatggcgta tgaatggcaa tttaatggtg tcaatgaata tagccgaatc
      241 gatatgagtc gattaaatgc agcagagttt aacgaccgct caaaaccaaa gccgaaaatt
      301 gaaggctcgt taaatgaggc ggaaattaaa gtttctaatg aattagcaga acgctttccg
      361 acttatttga acagtttgca tttggttgat gagaaaggca atcctttaac acttgatcca
      421 aaaggcaacg gctcgtttaa agattattta tctgaagtgg taaaaacggc tgcaaataaa
      481 gcctatagag gtttagttca agatagcgaa gagcaaaaag cctttcaaca aattagttgg
      541 ctttcatttg agaaaggcaa agtcagcagt gtggactggt ttggttatgt gttttcagat
      601 aaacggatga aatcaccacc tgcatttgat gcgttaaatg gaagttcggg cgagaataat
      661 ttatttggca cagacacgga aaataaccgc cattttacgc tttatagtgc ggaacgttct
      721 gccaataaag atctcaattt agccgatccg caaatcgtga aacggatgaa cccgatgcac
      781 tatttggata accgcaaatg ctgcggaaca ttggcggatt agggtgggaa cggcagatag
      841 agatacctcg ctggcaattt ctgcaatctt agcaataaaa ctgcaaatgg caggcaaaaa
      901 tgttaattat gaaaccccgt ggaatgtacc ccattcaggt gattatgatg taaatgaatt
      961 attcctatgg gctgatgagc tagtcaaagg caaaaagtaa aaaatagagt aaaataaagc
     1021 agatctgatg gtctgttttt tgttttgagg tcgtatgcaa gttaaaaaat ttagccaaaa
     1081 atatattaat tgggtattaa agctagggcg gttaaaagct tctcttttag gcttttttat
     1141 ggtcgctctt tcggcaattt taattcaaag tagcttaacc tatcttttta ttgggcgagt
     1201 agtcgtggtg gatattttac gctccatcgc ttttggttta atttctgcgc cttttgtgct
     1261 ttatttcttc acgttattgg tggaaaaact tgaaatatct cgcttgaaat tagaaaaagc
     1321 agtaagagat ttggaagaac tccgggaaca agatgcctat cttaacgcta aattagaaaa
     1381 aaacagccgt gataaaagca ttttaatggc gaccattagt cacgaacttc gtactcccct
     1441 taatggaatt atcggcttga gccaaatttt gttagatgat aaaaccaccg aaaaacagca
     1501 agaatatcta aaaactatca atatgagtgc cactgcattg gctcatattt ttggcgatat
     1561 tattgattta gaaaaaattg atagtagccg aattgaattg gtaagaaaag aggtggaatt
     1621 tgaacaactc attcacgata ttgctaattt tgggcatttt atggcgggca ataagcagat
     1681 taattttgaa gtgaagtatc cgcctgattt gccgaagtat atctatatcg accacgcacg
     1741 cttgagccaa attttatgga atttgatcag caatgcggtg aaatttaccc ctgcaagcgg
     1801 tcgaattttg ctggaaattt accaattaga taaagaacag tttagctttg cggtgacgga
     1861 ttcaggcatc ggtattccgg aaaaagatca acgcaaaatt tttgatatgt tctaccaagc
     1921 ggaaaactct aaagagaaaa aagcgcaagg tagcgggatt ggcttatcgg tctcgaaacg
     1981 cattgcgaag ttaatgggcg gtgatttggt ggtggcaagc caagaaaatc aaggctcaac
     2041 ttttaccctc accgtgcagg cggaaagcac cgaaaacaag ccgacatttg ctattcagca
     2101 gcattccctt aaggtgttat tggtggaaga tatcgaaatg aatgtggtgg ttgctcgagc
     2161 aatgttggaa aaaatcgggt gtgaggttga tgtggcaatg agtggtgaag aaacgtacca
     2221 acattttgca caggacagct acgatctgat tttacttgat attcaactgc cggataccac
     2281 cggttttgaa attgcacagc aattaagatt gcaatatgaa aacgaggaaa ttgactattt
     2341 accgctgttg gttgctttga cggcaaatgt gatccaaagc cggcaggagt accaagcaca
     2401 aggtatggac gatgtgctac acaaaccgct ctctttagat gaattaatgc gttgtctcaa
     2461 ccaacatttt ggagatgatt ttttgcaaaa aaatgaagaa aaacagccgc ttgtagagag
     2521 aggttctgag aatttacctt atagcattac aattcttaat gaattgattg gggtaatggg
     2581 gaaagagaat gtgcttgcca atatggattt atttgctgat ttaatgccca cttatttggc
     2641 aaatttacag cgttatttaa gtgattggca aaccacaaat tctgtggaaa gccgtaaatt
     2701 aacggcggac gaggctcata agattaaggg agcattagca tcagtcggat tacatcagct
     2761 acaacaaact gctcagcttg ctcaatccga taacggcgag cagtgggagc aaaatattgc
     2821 acactgggtg gaaatgattt gttccaaatg gcagatggat ttgcaattag ctaaggaata
     2881 tgttagaagc aagggggaca tttagtaaat ttgaaatgtt acgatggtgc aagcgtccac
     2941 gcttgtacca attttaatat ctgaagcaca agcgtggacg cttg
//
LOCUS       DANJWC010000204         2935 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.205, whole genome shotgun sequence.
ACCESSION   DANJWC010000204 DANJWC010000000
VERSION     DANJWC010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.205"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            63..1469
                     /gene="allB"
                     /locus_tag="PWX02_002266"
     CDS             63..1469
                     /gene="allB"
                     /locus_tag="PWX02_002266"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HDL1730234.1"
                     /translation="MYDLIVKNGQLVTPDRIYPATIAVKEGKFAGFLAPDIEIQAKEV
                     VDAKGHLVYPGIIDCHAHLNEPGFDYREDFETGSRAAVAAGCTTLIDMPLNNDPSLIN
                     KDVFKLKHDNVSKHSFIDFALWGGIVGDYDDSPNSIKSNSADLVELEACGVAAFKGFT
                     CPNGDLFPTVNLGNVRKALEILKPFNALSGFHCEEYGQVKERVKEAKAKIGLTNQEKI
                     REFLDAHDVWVEFVATKNIIDMSRATGGRVHICHVTHPMVAQLVKDAIYEGLPVSGET
                     CPHYLGFTEDFVFEKGGPAKCTPPLRKKEDMEKLWDYVIDGTLSCVGSDHSPAADEEK
                     NNDTHDIWEAWGGLNGIQFFLPMMFDMVVHQRKLSPSLIAKVMGVNPAKLFGLYGRKG
                     AFEIGFDADIVIVDPEKPWKVEQEKLFTKGHVTCFNGLEGKGAATHTFIRGRLVAENG
                     MYKQDAIGYGEFIRPVKR"
     gene            1565..2893
                     /locus_tag="PWX02_002267"
     CDS             1565..2893
                     /locus_tag="PWX02_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family transporter"
                     /protein_id="HDL1730235.1"
                     /translation="MSENTNLQPIPANKRIMDLLSYLFLWLGGCVSIGTFALGSGQLE
                     KGLNLTQAFVAMLIGSLLLIICLCLNDQMSYKSGVPYAVQLKSAFGTKGGILPAMLRG
                     VPAIVWYGVQSWLGGSAINQISILLVGYDNIVLYFLLFQALQIWISTTGFHGLKWLEN
                     IGAVVIIASLAYMFYVCMTKFGGRIEENLINKEGTWGLPFVSAIVAFFGVNVLVMLNV
                     GDYVRELKAGYSTTKRGVVYFLAMVPTTVFMGIIGLMISSATGIANPINAFAQAADNK
                     FLVVITLVFILFAQMTTNLASNVVPPAYALMDAFKIKHKTSVVLIGLLAIATCPWLIT
                     SDASSKGVDIFVLTYTVFFGPIFAVLLVDYYILRKQKVDIAELYDENGNRRGVNWAAI
                     LAISVGAVIGLFNADISFLTATIPTGLVYYFGMKYMASSARFRKGTILEK"
BASE COUNT          837 a          508 c          665 g          925 t
ORIGIN      
        1 acgattccac attttttatt ttcctttcaa accattaaaa gtcaataaaa agaaggttta
       61 ttatgtatga tttaatcgtt aaaaacggtc aattagttac acctgataga atttatcctg
      121 ccactattgc ggtaaaagag ggtaaatttg ccggtttttt agcgccggac attgaaatcc
      181 aagcgaaaga agtggttgat gccaaggggc atttggttta tccgggtatt atcgattgcc
      241 acgctcactt aaatgaaccg ggttttgatt atcgtgaaga ttttgaaaca ggctctcgtg
      301 cagctgtagc agcaggttgt acaaccttaa ttgatatgcc acttaataat gatccttctt
      361 taattaacaa agacgttttt aaacttaaac acgacaacgt gagtaagcat tcctttattg
      421 attttgccct ttggggcgga attgtggggg attatgatga tagcccgaac tccattaaaa
      481 gtaattccgc tgatcttgtt gagcttgaag cttgcggcgt agctgctttt aaaggattta
      541 cctgcccaaa tggggattta tttccaactg ttaatttagg gaacgtacgc aaagcgttag
      601 agatcttaaa gccgtttaat gccttatcgg gctttcactg tgaagaatat ggtcaagtaa
      661 aagagcgtgt gaaagaggca aaagcgaaaa taggcttaac caatcaggaa aaaatccgtg
      721 aatttttaga tgctcacgat gtgtgggtag agtttgtggc aacaaaaaat atcattgata
      781 tgtcccgtgc cacaggtgga cgagtgcata tttgccacgt tacacaccca atggtggctc
      841 agttggttaa agatgcgatt tacgaaggtt tgccagtgtc tggtgaaact tgcccacatt
      901 atttaggttt taccgaagat tttgtgtttg aaaaaggtgg ccctgcgaaa tgtacgccgc
      961 cgcttcgtaa aaaagaggat atggaaaaac tgtgggatta tgtgattgat ggtacgcttt
     1021 cttgcgtggg tagcgatcac tctccagcag ccgatgaaga aaaaaataat gacactcacg
     1081 atatttggga ggcttggggt ggtttaaatg gtattcagtt tttcctgccg atgatgtttg
     1141 atatggtcgt gcatcaacgt aaactcagtc caagtttaat tgccaaagta atgggtgtga
     1201 atccagcaaa actatttggt ttatatggtc gtaaaggggc ttttgaaatc ggttttgatg
     1261 cggatattgt gattgttgat ccagaaaaac cgtggaaagt agaacaagaa aaactcttta
     1321 ccaaggggca cgttacctgc ttcaacggct tagagggcaa aggtgcggca acgcatactt
     1381 tcattcgtgg tcgtttagtt gcggaaaacg gtatgtacaa acaagatgcg ataggctatg
     1441 gagagtttat tcgtccagtc aaacgctaga atttaagtgc ggtcaatttt tcaaaaatat
     1501 ctgaaaaaat gaccgctctt ttattatcat aaaaataaaa caatgttatt acaggagaga
     1561 cacaatgtct gaaaatacca acttacaacc aattccggca aataagcgga ttatggattt
     1621 actttcttat ctctttcttt ggctaggggg ctgtgtatct attggcacct ttgccctcgg
     1681 ttcaggacaa ttagagaaag ggctgaactt aacccaagct tttgttgcaa tgctaattgg
     1741 ttcattgttg ttgattattt gtttatgttt aaacgaccaa atgagctata aaagtggtgt
     1801 gccttatgcc gttcaattaa aaagtgcatt tgggacaaaa ggcggtattt tacctgctat
     1861 gctgcgtggt gtgccggcta ttgtttggta tggtgtgcaa agttggttag gcggttctgc
     1921 aatcaaccaa atttctattt tattagttgg ttatgacaat atcgtgcttt acttcttgct
     1981 attccaagca ctgcaaattt ggatttctac cactggcttc cacggcttga aatggttgga
     2041 aaacattggt gcggtagtga ttattgcctc tcttgcttat atgttttacg tttgtatgac
     2101 caaattcggt gggcgtattg aagaaaacct tattaataaa gaaggtacat ggggcttacc
     2161 atttgtgagt gcgattgtag cattctttgg cgtaaacgta ttagtaatgc ttaatgttgg
     2221 tgactatgtg cgtgagttaa aagccggtta ttcaactact aaacgtggtg tcgtttattt
     2281 cttagcgatg gtaccaacaa cggtatttat ggggattatc ggtttaatga tctcctcagc
     2341 aacaggtatc gcaaacccaa tcaatgcgtt cgcacaagca gccgataata aattcttagt
     2401 ggttattaca ttagtattta tcttattcgc tcaaatgacg accaaccttg caagtaacgt
     2461 tgtaccacct gcatatgcgt taatggatgc attcaaaatc aagcacaaaa cttcggtagt
     2521 gcttatcggc ttattagcga ttgcgacttg cccttggtta attacctcag atgcttcatc
     2581 aaaaggcgta gatattttcg tattaactta cacggtattc ttcggtccga tctttgcggt
     2641 gttattggtg gattactata ttttacgcaa acagaaagta gatattgctg aactttatga
     2701 tgaaaacggc aatcgcagag gggtaaactg ggctgcgatt ttagctatct ctgttggtgc
     2761 ggttatcggt ttatttaatg ctgatatttc attcctaaca gcgacaatcc caacaggatt
     2821 ggtttattac tttggtatga aatatatggc atcaagtgca cgtttcagaa aaggcacgat
     2881 tttggagaaa taagctcata accccatacg agccgtatgg ggttcaataa gctga
//
LOCUS       DANJWC010000205         2934 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.206, whole genome shotgun sequence.
ACCESSION   DANJWC010000205 DANJWC010000000
VERSION     DANJWC010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.206"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..196
                     /locus_tag="PWX02_002268"
     CDS             <1..196
                     /locus_tag="PWX02_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730236.1"
                     /translation="TPLVMSFIGVKEKIDKNYIINKQYYMTGKILLNQGEKFIFLEKG
                     KLYNESIVIDKENIKLEDSN"
     gene            196..684
                     /locus_tag="PWX02_002269"
     CDS             196..684
                     /locus_tag="PWX02_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HDL1730237.1"
                     /translation="MNIKKHIFPLLLISNTAFAAPVELHQFDSPKQESDYRALIQELR
                     CPQCQNNNIADSNATIATDMRAKTLELLKQGKSKDEVVDYMIERYGNFVTYDPPLTPA
                     TIMLWLLPLILIALGLGVVLRRKKNKPQAVNFEQNTAKPTTHLAQDEQERLNQILNKE
                     EK"
     gene            681..1592
                     /gene="ccmI"
                     /locus_tag="PWX02_002270"
     CDS             681..1592
                     /gene="ccmI"
                     /locus_tag="PWX02_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HDL1730238.1"
                     /translation="MNFWLAIIFVTLIVCGVAFYPLLKKNKQIDNTKRDSLNKAFYFD
                     RLKEVENEANEGVIDDPEQTKLELQKNLLDDIPHSTQSATSEKLTLGKSWFVALILFV
                     GGISFAIYSNVGSWFSNSMLDTTHQKLDSFYERLKTEETNPLSQEEMNQFAMALRVDL
                     QSNPNDDKKWYMLGQIGMALDDGQLAHDAFSKAAQLKPDNLDYKLSHAQLLLFSEAQE
                     DKKQGETLLKEIIRQDHTNFNALSLLAFHSFEKEDFKMAAMTWGMMLKLLPDEDPRKV
                     TVERSFNMALSNLSEAEKQELQKGEKK"
     gene            1589..2194
                     /gene="mog"
                     /locus_tag="PWX02_002271"
     CDS             1589..2194
                     /gene="mog"
                     /locus_tag="PWX02_002271"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HDL1730239.1"
                     /translation="MTACKPLSKEKLKIGLVSVSDRASQGVYSDQGIPELRAWLESAL
                     TEPFELETRLIPDEQAIIEQTLIELVDAHACHLVLTTGGTGPAKRDVTPDATLAVAHR
                     EMPGFGEQMRQVSLHFVPTAILSRQVGVIRHDSLILNLPGQPKAIKETLEGVKDEQGK
                     VLVKGIFSAVPYCLQLLSEIYVETHAAICESFRPKSARREN"
     gene            2204..2542
                     /gene="glnB"
                     /locus_tag="PWX02_002272"
     CDS             2204..2542
                     /gene="glnB"
                     /locus_tag="PWX02_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HDL1730240.1"
                     /translation="MKKIEAIIKPFKLDDVREALTDVGVSGMSITEIKGFGRQKGHTE
                     LYRGAEYAIDFLPKVKVEIVIPDELEEQCIEAIMETAQTGKIGDGKIFVYDVGRVIRI
                     RTGEENEEAI"
     gene            2623..>2934
                     /gene="lspA"
                     /locus_tag="PWX02_002273"
     CDS             2623..>2934
                     /gene="lspA"
                     /locus_tag="PWX02_002273"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDL1730241.1"
                     /translation="MKTGLKFLWVSLIVIIIDLWSKYIVVQHFEFGESINILPIFNFT
                     YARNYGAAFSFLADHSGWQKYFFLGLAVVISSALVVALWKNQAVKKLENIAYALIIGG
                     AI"
BASE COUNT          954 a          583 c          639 g          758 t
ORIGIN      
        1 gacccctctt gtaatgtctt ttataggagt aaaagaaaaa attgataaaa attatataat
       61 taataaacaa tattatatga caggtaagat tttattaaac caaggagaaa aatttatttt
      121 cttagaaaaa gggaaattat ataatgaatc aatagttata gataaagaaa acattaaact
      181 agaggactct aattaatgaa tataaaaaaa cacattttcc ctttactatt aattagcaat
      241 accgcctttg ctgcccctgt tgagcttcac caattcgaca gcccgaaaca agagtcggac
      301 tatcgtgcct taattcaaga actgcgttgc ccgcagtgtc aaaataataa tatcgccgat
      361 tccaatgcga ctattgccac cgatatgcgg gcaaaaacct tagaattgct caaacaaggc
      421 aaaagcaaag acgaagtggt ggattatatg attgaacgct acggcaattt cgtcacttac
      481 gacccgcctc tcacaccggc aaccattatg ttatggctgt tgccgttaat tttaattgcc
      541 ttagggctgg gggtggtgtt gcgtagaaag aaaaacaaac cacaagcggt caattttgag
      601 caaaatactg caaaaccgac cactcatctc gctcaagatg agcaagaacg tcttaaccaa
      661 attctcaata aggaagaaaa atgaatttct ggctggcaat tattttcgtt acactgattg
      721 tatgtggcgt ggcgttttat ccgttactta aaaagaacaa gcaaatcgat aacaccaagc
      781 gagacagctt aaacaaagcc ttctattttg accgcttaaa agaagtggaa aacgaagcga
      841 atgaaggggt gattgatgac cctgaacaaa ccaaattaga gctacaaaaa aacttgcttg
      901 atgatattcc acacagcaca caaagtgcca caagtgaaaa attaacgctc ggcaagtcgt
      961 ggtttgtggc attgatttta tttgtcggcg gtatcagctt tgcgatttac agcaatgtcg
     1021 gttcttggtt ctccaactca atgttagaca ccactcacca aaaattagac agcttctatg
     1081 agcgtttaaa aaccgaagag accaatccgc tctcacaaga ggaaatgaac caatttgcga
     1141 tggcattacg ggtagattta caaagtaatc cgaatgacga caaaaaatgg tatatgctcg
     1201 gtcaaatcgg gatggcgtta gatgatggtc agcttgcaca cgatgccttt agcaaagcgg
     1261 ctcaactcaa gccggataac ttagattaca aactcagcca cgcccaactg ctgctctttt
     1321 ccgaagcaca ggaagacaaa aaacagggtg aaaccttgtt aaaagagatt atccgccaag
     1381 accacacaaa ctttaatgcg ttaagtttat tggcgttcca ctcctttgaa aaagaagatt
     1441 tcaaaatggc ggcaatgacg tggggaatga tgttaaaact actgcctgat gaagatcctc
     1501 gcaaagtaac ggtagaacgc agttttaata tggcgttatc caatttaagt gaggcggaaa
     1561 aacaagaatt gcaaaaaggt gagaaaaaat gaccgcttgt aaaccactta gcaaagagaa
     1621 gttgaaaatc ggcttggtgt cggtttcaga ccgagcctcg cagggggttt attcagacca
     1681 aggcattccc gaattaaggg cgtggcttga atctgcctta accgagccgt ttgagctgga
     1741 aacccgctta attcccgatg agcaagccat tattgagcaa accttaatcg agttagtcga
     1801 tgctcacgct tgccatttgg tattaaccac cggcggcacc ggcccggcaa aacgtgatgt
     1861 caccccagat gccacccttg ctgtcgccca ccgagaaatg cccggctttg gcgaacagat
     1921 gcgacaagtg agccttcact tcgtgccaac cgccattctc tctcgccaag tgggagtaat
     1981 tcgccacgac agtctgattt taaatctgcc ggggcaaccg aaagcaatta aagaaacgct
     2041 ggaaggtgtg aaagatgaac agggcaaagt attggttaaa ggtattttta gtgccgtgcc
     2101 atactgttta caacttttaa gcgaaattta tgtcgaaacc cacgcagcca tttgcgaaag
     2161 tttcagaccc aaatcggcaa gacgagaaaa ttaaggctaa aaaatgaaaa aaatagaagc
     2221 aattattaaa ccttttaaat tagacgatgt gcgtgaagcc ttaaccgatg tgggtgtcag
     2281 cggaatgagt attaccgaaa tcaaagggtt tggcagacaa aaaggacata ccgagcttta
     2341 tcgtggggcg gaatatgcga ttgattttct accgaaagtg aaagtcgaaa tcgtgattcc
     2401 cgacgagttg gaagaacagt gcattgaagc aattatggaa accgcccaaa ccggcaaaat
     2461 cggcgatggc aagatttttg tgtatgatgt cgggcgggtg atccgcattc gtaccggcga
     2521 agaaaatgaa gaggcgattt agttttgcaa aaaatccggc aaaaacgacc gcttgcaggg
     2581 ggatttccat ctactctttt tacattaaat tttggatatt aaatgaaaac aggcttaaaa
     2641 tttctttggg tgagtttaat tgtaatcatc attgaccttt ggtcaaaata cattgtagtg
     2701 caacattttg aatttggtga aagcattaat attttgccga tttttaactt cacttacgcc
     2761 cgaaactacg gggctgcctt tagtttcctt gccgaccatt ccggctggca aaaatatttc
     2821 tttttagggc tggcggtagt gatttccagt gctttagtgg tggctctttg gaagaatcaa
     2881 gcggtcaaaa aattagaaaa tattgcgtat gccttaatta ttggtggagc gatt
//
LOCUS       DANJWC010000206         2885 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.207, whole genome shotgun sequence.
ACCESSION   DANJWC010000206 DANJWC010000000
VERSION     DANJWC010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.207"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            189..1583
                     /gene="murF"
                     /locus_tag="PWX02_002274"
     CDS             189..1583
                     /gene="murF"
                     /locus_tag="PWX02_002274"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDL1730242.1"
                     /translation="MIKLTTQQIAEILNAKLIGNGETVVETTSTDTRQAVENGLFFAL
                     KGENFDAHHYLQNAVAQGCVAVVVERECEIDVPQLVVADTRLALGELAKWLKAKLNPK
                     TVAMTGSSGKTTVKEMTAKILQKMTACEQEVLYTFGNLNNDLGVPMTLLRLTEQHKYA
                     VIELGANHIGEIAYTTAIAQPDACLVNNVAAAHLEGFGSLAGVAQAKGEIYRGLKENG
                     KAIVNLAFYYPQWQKEIDSREMQSFSYIGSDSDSYADYWAEDVELHLSGSRFTLHTPQ
                     GEIAIDLPYLGKHNVSNALAATSLAMSVGATLEAVKAGLEQRSMVKGRLYPVQINDHC
                     LFIDDSYNANVDSMKSAISVLENYPAYRIFAVGDMAELGEESSKCHQEVADFAKNAAL
                     DLIVSFGKESAVISQQAEHHFTDKQQMVDFLLPIILQKIEQKQPLVLLAKGSRSQKME
                     TVISELCKPFNLNF"
     gene            1604..2686
                     /gene="mraY"
                     /locus_tag="PWX02_002275"
     CDS             1604..2686
                     /gene="mraY"
                     /locus_tag="PWX02_002275"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDL1730243.1"
                     /translation="MLVWLAELLVQYNTAFNVVSYITFRAIMALLTAMGIGLWIGPKV
                     IRRLQILKFGQEVRNDGPESHFKKRGTPTMGGVMILAAIGVSTLLWADLRNSYVWFTL
                     FVLFGYGAVGFVDDYRKISRKNTDGLIARWKYFWLSAIALVAVFGMYAVGKDTAATQL
                     VVPFFKDVMPQLGLFYIVLSYFVIVGTSNAVNLTDGLDGLAIVPTIMVASAFALIAWA
                     TGNFNFAQYLHIPFIPNAGELVILCTAIVGAGLGFLWYNTYPAQVFMGDVGSLSLGGA
                     LGVIAVLVRQELLLVVMGGVFVVEALSVILQVGSYKLRQKRIFRMAPIHHHFELKGWP
                     EPRVIVRFWIITLMLVLVGLVTLKLR"
     gene            2750..>2885
                     /gene="murD"
                     /locus_tag="PWX02_002276"
     CDS             2750..>2885
                     /gene="murD"
                     /locus_tag="PWX02_002276"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249732.1"
                     /note="UDP-N-acetylmuramoylalanine--D-glutamate ligase;
                     involved in peptidoglycan biosynthesis; cytoplasmic;
                     catalyzes the addition of glutamate to the nucleotide
                     precursor UDP-N-acetylmuramoyl-L-alanine during cell wall
                     formation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDL1730244.1"
                     /translation="MQKQYENKTVTIIGLGTTGLSCVDFFKKKNANIQVIDTRANPAG
                     A"
BASE COUNT          824 a          531 c          688 g          842 t
ORIGIN      
        1 aaataacccg agcgagagcg aagcgaacat cagggttatt tttcgttaag aaaatttcaa
       61 ccaagtgagg atagcaacga agcagggtgt gctttctttt ggttactttt ctttgcacaa
      121 gcaaagaaaa gtgacagtga caaatacaaa atgctcgtaa ttaattttat aaagaaaaag
      181 ataaatcaat gataaaacta accacccaac aaatcgctga aatcctaaat gccaaattga
      241 tcggcaatgg cgaaactgtg gtagaaacca ccagcaccga cacccgacaa gcggttgaaa
      301 acggcttatt ttttgcatta aagggcgaga actttgatgc tcatcactat cttcaaaatg
      361 cagtcgcaca aggttgtgta gcggttgtgg ttgagcgtga gtgtgaaatt gatgtaccac
      421 aacttgtggt ggcagatacc cgtttggcat tgggggaatt ggcgaaatgg ttaaaagcta
      481 agctgaaccc gaaaacggta gcgatgacag gttcttccgg caaaacaacc gtcaaagaga
      541 tgacggcaaa aattctgcaa aaaatgaccg cttgcgaaca ggaagtgctt tatacttttg
      601 gtaacttaaa caatgactta ggtgtaccga tgaccttgct tcgcttaacc gaacagcata
      661 aatatgcggt cattgagctg ggggcaaatc atatcggtga aatcgcttat accaccgcta
      721 tcgctcagcc tgatgcctgc ttggtgaata atgtggctgc ggcgcattta gaggggtttg
      781 gttcattagc aggcgtggca caagccaagg gcgaaattta tcgtggcttg aaagaaaatg
      841 gcaaagcgat tgtcaattta gctttttatt atccgcaatg gcaaaaagag attgactcac
      901 gggaaatgca atctttctct tatatcgggt cagattcgga ttcttacgca gattactggg
      961 cggaagatgt agaactgcat ttaagcggtt cacgctttac gctacatacc ccacagggcg
     1021 aaattgctat tgatctgcct tatttgggca aacataacgt gagcaatgcc ttagcggcaa
     1081 cttcgctggc gatgtctgtt ggggcaacgc tagaagcagt taaggcaggg cttgagcagc
     1141 gttcaatggt aaaagggcgt ttgtatccgg tgcaaattaa cgaccactgt ttgtttattg
     1201 atgactctta caacgccaat gtggattcaa tgaaatcggc gatttctgtt ttggaaaatt
     1261 atccggctta tcgtattttt gcagtgggtg atatggcgga attgggtgaa gaaagtagca
     1321 aatgccacca agaagtagcg gattttgcca aaaatgcagc attagattta atcgtgagct
     1381 ttggaaaaga aagtgcggta atcagccaac aggcagaaca tcattttacc gataaacagc
     1441 aaatggttga tttcctactg ccgattattt tgcaaaaaat cgaacaaaaa caaccgcttg
     1501 tattattagc aaaaggctct cgtagccaga aaatggaaac cgtgatttcc gaattgtgta
     1561 aaccgtttaa cctaaatttc taattatttt taaggactaa gcaatgttag tttggcttgc
     1621 agaactattg gttcaatata acacagcgtt taacgttgtc tcttatatta ctttccgtgc
     1681 cattatggca ttattaactg caatgggaat cgggctttgg atcgggccga aagtaattcg
     1741 ccgtttgcag atcttaaaat ttggtcagga agtgcgtaat gacggaccgg aaagccattt
     1801 caaaaaacgt ggtacgccaa caatgggcgg agtgatgatt ctcgcagcga ttggggtgag
     1861 tacattgctt tgggcagatc tgcgtaattc ctatgtttgg ttcacgctgt ttgtgctatt
     1921 tggctacggt gcagtgggct ttgttgatga ttatcgtaag atctctcgta aaaataccga
     1981 tggtttgatt gcccgttgga aatatttttg gttatctgct atcgcattag tggcggtgtt
     2041 tggtatgtat gcggtgggta aagataccgc tgcaacacaa ttagtcgtgc cgttttttaa
     2101 agatgtgatg ccgcaactcg gtttatttta tattgtgtta tcctatttcg tgattgtcgg
     2161 caccagtaat gcggtaaacc taacggacgg cttagacggg cttgcgattg tgccaactat
     2221 tatggtcgca tctgcctttg cgttaattgc gtgggcaacc ggtaacttta attttgcaca
     2281 atatttacat attcctttta ttccaaatgc cggtgaattg gttattttat gtactgcgat
     2341 tgtgggagca ggtttagggt tcttatggta taacacctac cctgcacagg tgtttatggg
     2401 cgatgttggc tcactttcgc tcggcggtgc attaggcgtg attgcagtat tagttcgcca
     2461 agaattatta ttggtggtaa tgggcggtgt atttgtggtt gaagccctct cggtgatttt
     2521 acaagtaggt tcatacaaat tacgccaaaa acgtattttc cgaatggctc caattcacca
     2581 ccattttgaa ctcaaaggct ggcctgaacc acgggttatc gtgcgtttct ggattatcac
     2641 gctaatgctg gttttagtcg gcttagtaac attgaaatta cgttaattga ttgaataagg
     2701 tgcattgaga taatgtacct tatgttattt ttaaattaca gaataaatta tgcaaaaaca
     2761 atacgaaaac aaaaccgtta ctattatcgg cttaggtaca acagggttat cttgtgtgga
     2821 tttctttaag aaaaaaaatg ccaatatcca agtgattgac acccgagcaa acccagccgg
     2881 agcgg
//
LOCUS       DANJWC010000207         2865 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.208, whole genome shotgun sequence.
ACCESSION   DANJWC010000207 DANJWC010000000
VERSION     DANJWC010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.208"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(512..1024)
                     /locus_tag="PWX02_002277"
     CDS             complement(512..1024)
                     /locus_tag="PWX02_002277"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730245.1"
                     /translation="MGKINYFDDIKIGNEIFSLSHLNPKYLEFYGQKVQKTLKLKVLY
                     SDHCFTARVNRLQSDSERVFSIERYNLSKLLPNIIEGLNNDKIQVFQTLARRNFAYVV
                     KTTLNDKDYNIFFEVKKSNNSNPDCDLVIRIESAYVFDNDCKLEYSGKIRFLILCQKI
                     YQGEKIQCRK"
     gene            complement(1024..1242)
                     /locus_tag="PWX02_002278"
     CDS             complement(1024..1242)
                     /locus_tag="PWX02_002278"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730246.1"
                     /translation="MATIVQKDVLIEAIAQVQGYLLRSLPSSDSMNDDELFLCELREK
                     IYNTHHDKLDYESLLADIVKIKNKSCYS"
     gene            complement(1253..1450)
                     /locus_tag="PWX02_002279"
     CDS             complement(1253..1450)
                     /locus_tag="PWX02_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="HDL1730247.1"
                     /translation="MSNEELTPEVLARRAYHVRNALASFSLEGEYPSKEAEDLFNKFA
                     SGEIETIDELRVQINLLYSED"
     gene            complement(1458..1664)
                     /locus_tag="PWX02_002280"
     CDS             complement(1458..1664)
                     /locus_tag="PWX02_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730248.1"
                     /translation="MATLMEKDSLINGISQTLGLIVAQIGDRNEDSVMLATLRNKLYG
                     LDAEKIDYQGTAKLVKEKFNLYKL"
     gene            complement(1674..2651)
                     /locus_tag="PWX02_002281"
     CDS             complement(1674..2651)
                     /locus_tag="PWX02_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL1730249.1"
                     /translation="MANDLVVVKANSLIEASYRLSIDEIRILALTIGTMDPKSNQQIF
                     DFTVADFVREFPEISMDNAYKQIQAAIKRIYERSVRTEDSNRVTEFRWVSSRTYFKNE
                     GRFRIAMTNEVMPYLTQLKGQFTQYQLRHISSFNSVHSIRIYELVTQYRSVGYREITI
                     EDLKKWLQVEDKYPRFNNFKQWVLEPSITEINDKSDLLVDVEQIKRGRSIIALKFTIK
                     SKKSAVKAELKRPPFPHKNKYGKFVTLNRQDPRMSNHEYGLWAKDCLKILEGFYQKIE
                     DIPNEDLLFYWIFLTGNASNKSKLGTRKNFVDELKKRGYKIEHCELVKV"
BASE COUNT          931 a          525 c          414 g          995 t
ORIGIN      
        1 aaaaaaaaca aacctaaaac aagtaaaaaa ctaaaaaact aaaatataga gcctatcggc
       61 tcttttcggg ctttcagccc tattttttta tcttttttac actagaaaaa cctttttaaa
      121 ctttggtaat accaaataca acctaataat actaaataaa acctttttta tacctaaata
      181 ctacgttgtg tatattaaaa gtttacttaa atgagtgggc aagatatgta aactagacct
      241 actttttgca aagtatatct agtttactcg gataaattcg ctacagctca aatcctatat
      301 cttgtccttc ggagttttta caccaggagt gatgaatagg catcaatagc aaacttgaca
      361 tattgagaaa agacgccata atactaatac aacctatggc aacccgatga aatcgatcat
      421 aggacggctc agaaatgagt aaggtgcgat gccttggtaa gtgtattcgg ggtctattta
      481 agagaaaagc cacgttttaa cgtggctttt ctcattttcg gcattgtatt ttttcacctt
      541 gataaatttt ttggcaaagt atcaaaaatc gaatctttcc tgaatattcc agtttacaat
      601 cattatcaaa tacataagca ctttctatac gaataactag atcacaatct ggattgctgt
      661 tgttactttt tttgacctca aaaaatatat tgtaatcttt atcgtttagc gtagttttga
      721 ctacgtatgc aaaattacgt ctagctaacg tctgaaaaac ttgaattttg tcgttattta
      781 gcccttctat gatgtttgga agtaattttg ataggttata tctctctata gagaaaacac
      841 gctcagaatc gctctgtaag cgatttacgc gagcagtaaa acagtgatcg gaatacagaa
      901 cctttaattt aagcgttttt tgtacttttt gaccgtaaaa ttcaagatat ttaggattga
      961 gatgagagag tgagaaaatc tcattaccaa tttttatatc atcaaagtaa tttatctttc
     1021 ccattaacta taacaactct tatttttaat ttttacaata tcagcaagta atgattcata
     1081 atctagttta tcgtgatgag tgttataaat tttctcacgt agctcacaca aaaatagttc
     1141 atcatcattc atggagtcag aggagggtaa gctacgtaat aaataacctt gaacctgggc
     1201 aatcgcctca attaaaacat ctttttgaac tatagttgcc atttttacct tcctaatctt
     1261 cagaatacaa taaattaatt tgaactcgca attcatctat tgtttcaatt tcaccagaag
     1321 caaatttatt aaatagatcc tccgcctctt tagagggata ttccccttcc aaagagaaac
     1381 tagccagggc atttcttaca tggtatgccc gtcgagctaa aacttctggt gttaattctt
     1441 cattactcat aaaactccta tagcttatag agattaaatt tttcttttac aagttttgca
     1501 gttccttgat aatcaatttt ctctgcatca agtccatata acttattacg caatgttgct
     1561 aacataacac tatcctcgtt tctgtcgcct atttgagcta cgattaaacc tagtgtttga
     1621 gaaataccat ttatcaagct gtctttttcc ataagtgttg ccatattacc ctcttaaact
     1681 ttaaccaatt cacaatgttc tatcttgtat cctcgttttt taagttcatc aacaaagttt
     1741 tttcttgttc ctaattttga tttattgctt gcattacctg ttaggaaaat ccaataaaac
     1801 agcaaatctt cattcggtat atcttcgatt ttttgataaa aaccttccaa tatttttaaa
     1861 cagtcttttg cccataaacc atactcatga ttgctcattc ttggatcttg tcggtttaac
     1921 gttacaaact taccgtactt attcttatgt gggaatggtg gtcgttttaa ttctgctttg
     1981 accgcacttt tcttactctt aatcgtaaat tttaatgcaa taatactacg accacgttta
     2041 atttgttcta catcaacgag cagatcagat ttatcgttaa tttccgttat agatggctct
     2101 aaaacccatt gtttgaaatt gttaaaccta gggtatttat cttccacctg taaccatttt
     2161 ttcaaatctt caatagtgat ttcacgataa ccgacagagc ggtattgtgt aacaagctca
     2221 taaatacgga tagagtgaac actattaaaa cttgaaatgt gcctaagttg gtactgcgtg
     2281 aattgtcctt ttaactgcgt aaggtatggc attacttcgt tagtcatagc aatacgaaaa
     2341 cgcccttcat ttttgaaata agtgcgagaa gatacccagc gaaattcagt tacacgatta
     2401 ctatcttcgg ttctaacact acgttcataa atccgtttga tagccgcctg aatttgctta
     2461 taagcgttat ccatactgat ctcaggaaat tcacggacaa aatcggctac cgtaaaatca
     2521 aaaatttgct gatttgattt tggatccatt gttccaatcg ttaaagctag aatacggatt
     2581 tcatcaatac ttaatcggta actagcttca ataagactat ttgctttcac tacaactaaa
     2641 tcatttgcca taagactaga tatttcctgt taaaaaataa cctttaaaac tagataaata
     2701 ataacaccta gctaattaaa aagactagat aaattttaac atcaagtcga taacaggaaa
     2761 tttctagtcg tataacagga aatttctagt cgtataacag gaaatttcta gtcgtataac
     2821 aggaaatttc tagtcgtata agtttctaac ccattgtaat aaaag
//
LOCUS       DANJWC010000208         2787 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.209, whole genome shotgun sequence.
ACCESSION   DANJWC010000208 DANJWC010000000
VERSION     DANJWC010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.209"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..2231
                     /locus_tag="PWX02_002282"
     CDS             <1..2231
                     /locus_tag="PWX02_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDL1730250.1"
                     /translation="LMKTSDKIIEGKEFKKRSATLGNALAAELGVHSNPFGGGASKPI
                     IRGQEGARIRILQNGSDVIDMSNLSPDHAVVADSLLADQVEILRGSSTLLYASSSPVG
                     IVNVVDKRIPTAIPEKGYEVELNSRFDTAAKEKVGALGATFGIGNHIAVRAEGLTRHS
                     DNYRVPGINLGERLNYVPDTYNKSKVGTLGLSFVGERGYIGASYSKRRDNYGLPGHNH
                     KFDFCTGHIYGNKRDKYAYTYLYPHLIGEENIGSNPHFHCGTNHAEDGTHSHDNPFGH
                     AHDHTHKGPWVDLESKRIDVKAELRQPFKGVDKIKASYADADYYHDEKDAGVLATRYH
                     KQLKKDQDYGKPVNIFKNRGKNTRLEVYHAPLGGLTGVWGVQYQTQKSSMNAPKDREV
                     KFPLVENTNKQFSLFGVEQYMWDSVAVELAGRMEKQKIEIEYDRNEIKRLQEHYRISG
                     GKQVEPDLSPYDETAYAYSGTLNWFFHPDYQLSFTASHNERLPTPMELYYHGQHIATN
                     SFEYGNKDLKKEQSNNVELGLGYQAERIGYKVSVYYNHFKNYIYNENLFRENQLFMRR
                     YNQAKARFYGVEAEASYRFNDQYQATIFGDMVRGWLTNLPPLKINSDYSVFKDYLPEN
                     VEKGKDYLLYRADQNTPRTPPMRLGFRFNAEFTPNWSGDLELIRTFTQRRTSQLEYIT
                     EGNTMLNVGLSYSNKWKELDYKISLNGTNLLNQPVYIHTSYHQFVPQMGRNFMLGMEM
                     KF"
     gene            2588..>2787
                     /locus_tag="PWX02_002283"
     CDS             2588..>2787
                     /locus_tag="PWX02_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730251.1"
                     /translation="MNKSLMVTKRNGTREQLNLDKIHRVITWAAEGLENVSVSQVELR
                     SHIQFYEGIRTSDIHETIIKAAA"
BASE COUNT          842 a          553 c          642 g          750 t
ORIGIN      
        1 ccctaatgaa aaccagtgat aaaatcattg aaggtaaaga gtttaagaaa cgttcggcaa
       61 cattgggcaa tgcgttagcc gctgagttag gcgtgcatag taacccgttt ggtggcggag
      121 cgagtaagcc gattattcgt gggcaggaag gggcgagaat tcgcattttg caaaacggtt
      181 cagacgtgat tgatatgtcg aacctatctc ctgatcacgc cgttgttgct gattcactgc
      241 ttgccgatca ggtcgaaatc ttgcgtggtt ccagcacctt gctttatgcc agcagttcgc
      301 cggtaggaat tgtaaatgtg gtggataaac gtattccgac cgctattcct gaaaagggat
      361 atgaggttga actcaatagc cgttttgata ccgccgccaa agagaaagtc ggcgcattag
      421 gggcaacctt tggcatcggt aaccacattg cggtgcgagc cgaagggctg acccgccata
      481 gtgataacta ccgtgtgccg gggatcaatt taggcgagcg cttaaactat gtgccggaca
      541 cttacaataa atccaaagtc ggcacgttgg gcttatcctt tgtcggtgaa cgaggctata
      601 ttggtgcctc atacagcaaa cggcgggata attatggctt gccggggcat aaccataaat
      661 ttgatttctg taccggtcat atctacggta ataagcggga taaatacgct tatacctatc
      721 tctatccgca tttgattggt gaggaaaata ttgggagcaa tccgcacttc cactgtggaa
      781 ccaaccacgc cgaggacggc acacacagcc acgataaccc gttcggacac gcccacgatc
      841 acactcacaa aggcccgtgg gtggatttag aatcaaaacg gattgatgtg aaagccgagc
      901 tacgccagcc gtttaagggc gtagataaaa ttaaggcaag ctatgctgat gccgattatt
      961 accacgatga aaaagatgca ggcgtgcttg ccacccgtta ccataaacag ctgaaaaaag
     1021 atcaggatta cggcaagccg gtgaatattt tcaaaaatcg gggcaaaaat acccgcttgg
     1081 aggtctatca cgcaccgcta ggtggcttaa ccggtgtatg gggtgttcag tatcaaacgc
     1141 aaaaaagcag tatgaatgcg ccgaaagatc gcgaagtgaa attcccgctg gttgaaaata
     1201 ccaataaaca gttcagcctg tttggggttg agcagtatat gtgggatagc gttgctgttg
     1261 agcttgccgg acgaatggaa aagcagaaga ttgagattga gtatgatcgc aatgaaatca
     1321 aacggttaca agaacactac cgtatcagtg gcggcaaaca ggttgagcca gatctttcac
     1381 cctatgatga aacggcttat gcctattccg gcacgcttaa ctggttcttc caccctgact
     1441 atcagctttc gtttactgcc tcgcacaatg aacgcttgcc aacgccaatg gagctgtatt
     1501 atcacgggca gcatattgct actaattcgt ttgaatacgg caataaagac ttgaaaaaag
     1561 agcagtcaaa taatgtggag ttgggcttgg gctatcaagc tgaacgtatt ggctataaag
     1621 tgagtgttta ttacaatcac tttaaaaact acatctacaa tgaaaatctt ttccgtgaaa
     1681 atcagctatt tatgcgccgt tataatcagg caaaagcccg tttttacggg gtagaagcag
     1741 aggcaagcta ccgctttaac gaccaatacc aagccacgat tttcggcgat atggtacgag
     1801 gctggctaac aaatttacca ccattgaaga taaatagcga ttatagtgtg ttcaaagatt
     1861 atctgccgga aaatgtggaa aaagggaaag attatcttct atatcgggct gaccaaaaca
     1921 cccctcgtac accgccaatg cgtttaggtt ttcgctttaa tgcggaattt accccaaatt
     1981 ggtcggggga tttagagctg atccgcacct ttacccaacg ccgtacgagc cagttggaat
     2041 atattaccga aggtaatacg atgttgaatg tcgggttgtc gtatagcaat aagtggaaag
     2101 agctggatta caaaatcagt ctaaacggca caaacttact gaatcagccg gtgtatatcc
     2161 acacttcgta tcaccaattt gtaccacaaa tgggacgaaa ttttatgttg ggaatggaga
     2221 tgaaatttta agttctcgac ataacaagac aagcggttat tttttcttca aattttgcaa
     2281 aaaaacggct aaaaataacc gcttgtttaa aactcaggta ttggtaattt ggataaaata
     2341 gtgctgtttt tcctttcttg ttattttcgg agcttaccaa aatttcctgt ttgttgttaa
     2401 ataagacaat taactttatc cctttattaa aaatatggtt atttataaat aacttagcat
     2461 ctgtcaagag aaaaagtaaa aatttattgg ttgatgtttt gctttaagca cactatcttg
     2521 tgtttacttt aatgaaaaac acaagatgta gtgttttact aaggtatttt ataaagggtt
     2581 aataattatg aataaaagtt taatggtaac aaagcgtaat ggcacgcgcg agcaactcaa
     2641 tctagataaa attcatcgtg tgattacttg ggcggcagaa gggttagaga atgtttctgt
     2701 ttcacaagta gaactccgtt ctcacattca attttatgaa ggtattcgca cttcagatat
     2761 tcatgaaact attatcaagg ctgcagc
//
LOCUS       DANJWC010000209         2738 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.210, whole genome shotgun sequence.
ACCESSION   DANJWC010000209 DANJWC010000000
VERSION     DANJWC010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.210"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            246..1610
                     /gene="glmU"
                     /locus_tag="PWX02_002284"
     CDS             246..1610
                     /gene="glmU"
                     /locus_tag="PWX02_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDL1730252.1"
                     /translation="MTQLSVVILAAGKGTRMYSDLPKVLHPVAGKPMVKHVIDTAKQL
                     DAQQIHLIYGHGADLLKAHLSEEPVNWVFQAEQLGTGHAMQQAAPFFADDENIVMLYG
                     DAPLITKATLERLIAAKPENGIALLTVELENPTGYGRIIRENGSVVAIVEQKDANEAQ
                     LKVKEVNTGVMVASGASFKKWLKNLNNNNAQGEYYITDVIAMANQEGFKVQAVQATEF
                     MEVEGANNRLQLAALERFYQKREAEKLLLAGVAIIDPSRFDLRGTVTHGKDVQIDVNV
                     ILEGEIKLGNNVKIGAGCVLKNCEIGDNVEIKPYSVIEDSIVGAKSAIGPFSRLRPGA
                     ELAEETHIGNFVEIKKATIGKGSKVNHLTYVGDAEIGKECNIGAGVITCNYDGANKFK
                     TIIGDNVFVGSDSQLIAPVTIASGSTIGAGATVTKDIAENELVISRVPQRHIQGWQRP
                     TKKK"
     gene            complement(1763..>2738)
                     /locus_tag="PWX02_002285"
     CDS             complement(1763..>2738)
                     /locus_tag="PWX02_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDL1730253.1"
                     /translation="FDGSLRIVLDRTNSKELNNHNVVKTKKDAHTGIRNTGSRFGVRV
                     KHDLAQDFYALGRVEFRFNKNTSDDGFGDLYTKRAYVGLGSKQYGELTFGRQLTIADD
                     LSQSVDYNYGINPKGDYIQTDGNQVIRYNYRGVENLHVSANYNFAQDRKDGEVLANKL
                     QNAYGIGALYTVDNWDLRAAYGHTNYKTATDSTKHRKDGVLGSVGYKFGDLKLSVDSG
                     YRHEKVENEKGNAFYVSPNFEYQVVPASKVYGNYLYEREKVKKVSKEKTHGFLLGVDY
                     QFHKQVVAFVEGSYTRTSTKDYNKVTNGYKYTGKSTNKAIGVGMRVYW"
BASE COUNT          912 a          531 c          501 g          794 t
ORIGIN      
        1 aatttaatat tttagcaaag atatacacta ttgtagagag atagcacaaa taagtgctac
       61 ctatttatac cagttttcca caagataaga atcgacataa tagaaaaaga taacaaacag
      121 acaaatttct tttgcttgtc gatgaaatcc acttataaaa aggttaaaat attacaaaaa
      181 tatcacttat agcaatttac acgttactat aagccttcat ctatttagtc aaataaggat
      241 tctatatgac tcaactgagc gttgttattt tagctgccgg taaaggcact cgtatgtatt
      301 cagatttacc caaagtgtta cacccagttg ccggtaaacc catggtaaaa cacgttattg
      361 atactgccaa acagctagat gcccagcaaa ttcatttaat ttacggacac ggtgccgatc
      421 tgttaaaagc acatttaagc gaagagcctg taaactgggt attccaagcc gaacaattag
      481 gcacaggaca tgcaatgcaa caagcagctc cttttttcgc tgatgatgaa aacattgtaa
      541 tgctttatgg tgatgccccc ctaatcacaa aagccacttt agagcgttta atcgcagcca
      601 aaccggaaaa tggtattgca ttactaaccg ttgaattgga aaacccgaca ggctacggac
      661 gaattattcg tgaaaatggt tctgtggtag cgattgttga gcaaaaagat gccaatgaag
      721 cacagcttaa agtcaaagaa gtgaataccg gcgtgatggt agcaagcggt gcgagcttca
      781 aaaaatggtt gaaaaaccta aataacaaca atgctcaagg tgaatattac atcactgatg
      841 tgatcgcaat ggcaaaccaa gaaggcttta aagtacaagc ggttcaggca actgaattta
      901 tggaagtaga aggggcaaac aaccgtttgc aacttgctgc acttgaacgg ttctaccaaa
      961 aacgtgaggc ggaaaaactg ttattggctg gtgtagctat tatcgaccca agccgttttg
     1021 atcttcgtgg cactgtcacc cacggaaaag atgttcagat cgatgtgaat gtcattctag
     1081 aaggtgaaat caaactcggt aacaatgtaa aaattggtgc aggctgcgta ctgaaaaact
     1141 gcgaaattgg cgataatgtt gaaattaaac cttattctgt gattgaagat tcgattgtcg
     1201 gggcaaaatc cgctatcggt ccattctctc gcttacgccc gggggcagaa ttagcggaag
     1261 aaacccatat cggtaacttt gtggaaatca aaaaagctac catcggcaaa ggttcaaaag
     1321 taaatcatct cacttatgta ggcgatgcgg aaatcggcaa agaatgtaac atcggagcag
     1381 gtgtaatcac ttgtaactat gatggtgcga ataaattcaa aaccattatt ggcgataacg
     1441 tatttgtcgg ctcagacagc caactcattg ccccggtcac gattgccagc ggttcaacca
     1501 ttggtgccgg tgcaacagtc actaaagaca ttgcggaaaa tgaactggtt attagccgtg
     1561 tgccacaacg ccacattcaa ggctggcaac gcccgactaa aaagaaatag tgccagtggt
     1621 caaaagttat tatatttaaa aattggcttt gctaatactt gcaaagccat acgcttaaaa
     1681 aaacataatg aaccatatat ttaaaacaaa acccctaaca ataaatattg ttaggggttt
     1741 atcaatttag gaaatcaatt gattaccagt atacacgcat accaacaccg atagctttgt
     1801 tagttgattt gcctgtgtat ttataaccat tagttacttt attgtaatcc ttagtactag
     1861 tacgagtata agaaccctca acaaaagcaa ctacttgttt atggaattgg tagtcaacac
     1921 ctaataagaa gccatgagtt ttttctttac ttaccttttt cactttttca cgctcatata
     1981 ggtagttacc atatacttta gatgctggta ctacttggta ttcaaagttt ggagatacat
     2041 agaaagcatt tcctttctca ttttctactt tttcatgacg atagccagag tcaacactta
     2101 attttaagtc accaaattta taaccaacag aacctaatac accatcttta cggtgcttag
     2161 tgctatcagt agctgttttg tagtttgtat gaccataagc tgcacgtaaa tcccagttat
     2221 ctacagtgta taatgcacca atgccgtatg cattttgaag tttatttgct aatacttcac
     2281 catctttacg atcttgtgca aagttatagt ttgcacttac atgtaagttt tcaacaccac
     2341 ggtagttata acgaataact tggttaccat cggtttgaat ataatcgcct tttggattaa
     2401 taccgtagtt gtaatctact gattgactta aatcatcagc aatcgttaat tgacgaccaa
     2461 aagttaattc accatattgc ttactaccta aaccaacata agcacgttta gtatataaat
     2521 caccaaaacc atcatcactg gtatttttgt taaaacggaa ctcaacacga cctaatgcat
     2581 agaaatcttg agctaaatca tgttttactc ttacaccaaa acgtgaacca gtgttacgga
     2641 ttcctgtgtg tgcatctttt ttagttttaa caacattatg attgtttagc tctttagaat
     2701 ttgttctatc aagaacaata cgtaaagaac catcaaaa
//
LOCUS       DANJWC010000210         2717 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.211, whole genome shotgun sequence.
ACCESSION   DANJWC010000210 DANJWC010000000
VERSION     DANJWC010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.211"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..547)
                     /locus_tag="PWX02_002286"
     CDS             complement(<1..547)
                     /locus_tag="PWX02_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein Hlp"
                     /protein_id="HDL1730254.1"
                     /translation="MNTLAKISLSVLFTLFLTACEQPNSTKTQSSAESPVQVKEESKE
                     EVKPADTGAQDYKMLREWQDTQEKALNDAIKAATETLTDKQKADSTLMQETVNNALLA
                     QIDHIKVSAETLNIQNNEVKALKDKTLEVLTLGSQMIVEGAKMEKNPTPEAHKAFGEL
                     QTKLNQLAEEGQQLENILRAKY"
     gene            complement(614..1198)
                     /locus_tag="PWX02_002287"
     CDS             complement(614..1198)
                     /locus_tag="PWX02_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein HlpB"
                     /protein_id="HDL1730255.1"
                     /translation="MNKLTKISATALVALFLAACDKPAEKAPATEPTKTTEAAPTTTA
                     PAAEAAKPAETAVAAPSEAAKADYQKLVDWNTAQESTMATAQAELQKKLATQDPKQIQ
                     EGLSAFNKKVEETVKSLDAIEVSDAQIKTFKEKTKSVLTLSSEVISEQVKTISTPNDQ
                     AALQAVQQKAQSLIEAGNELQKLNVELQQRFSAK"
     gene            complement(1330..2514)
                     /locus_tag="PWX02_002288"
     CDS             complement(1330..2514)
                     /locus_tag="PWX02_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HDL1730256.1"
                     /translation="MKSADIVIVGGGMVGLALAGLLKDVDCQIKIIEKNTPKFDADTI
                     YHRVSAINASSQKILEQIGAFQHIPAQRLSPYTEMKVWEKDSFANIHFDNNDNEIKQL
                     GLSQLGFILENAVIQHALWQQVSQQANVEYIIAAPQTLGVSENGAFLTLDNGEMLSAK
                     LVVGADGANSWVRNQMKMPLTSKDYQHTALVCNVKTTEKHGKTARQIFAEDSIFAFLP
                     MADEHLCSIVWSLPPNKANELKNASEAEFNKALTIAFDNQLGLCEVQSLREIYPLAAR
                     FCRDFAQPRIALIGDAAHTIHPLAGLGVNLGFADARTLANEIQKQLVNNQDIGEYRHL
                     RQFERERKAQAMKLLATMEGLKQLFDGNHPLKKLFRGIGLSLTNQLPFVKKQLIQQAI
                     YL"
     gene            complement(2597..>2717)
                     /locus_tag="PWX02_002289"
     CDS             complement(2597..>2717)
                     /locus_tag="PWX02_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNase adaptor protein RapZ"
                     /protein_id="HDL1730257.1"
                     /translation="GGKHRSVFIAEQLAKYFQAKGKNVQVRHKSLEKHHKKTS"
BASE COUNT          693 a          520 c          540 g          964 t
ORIGIN      
        1 catatttagc tcttaatata ttttctaact gttgcccttc ttcagctaat tgattcaatt
       61 ttgtttgtaa ctcgccaaat gctttatgtg cttccggtgt aggatttttc tccattttag
      121 caccttctac gatcatctga gaccctaaag tcaggacttc taaggttttg tcttttagtg
      181 ctttgacttc attattttga atattcaggg tttcagcact taccttaata tgatcaattt
      241 gagctaataa tgcgttatta actgtctctt gcattaaggt agaatcggct ttttgtttgt
      301 cagtaagtgt ttctgttgcc gctttaattg catcatttaa ggctttttct tgagtgtctt
      361 gccattcccg taacatttta taatcttgag cgccggtatc agcaggttta acttcttctt
      421 ttgactcttc tttcacttga actggcgatt ctgctgagct ctgtgttttg gtagaattag
      481 gttgttcgca agcggttaaa aaaagggtaa ataatacaga taggctaatt ttagcgagcg
      541 tgttcatagt aatccttagt aattgcaaaa aaaagctaga gcaaaatact ctagcttttc
      601 attgaatggt aaattattta gctgagaatc tttgctgtaa ttctacgttt aatttttgta
      661 actcattacc agcttcaatt aaagactgag ctttttgttg tacggcttgt aatgctgctt
      721 ggtcatttgg tgtagagatc gtttttactt gttcagaaat cacttcactt gaaagtgtta
      781 aaactgattt tgttttttct ttaaatgttt tgatttgtgc atcagatact tcaatcgcat
      841 ctaagctttt tacagtttct tctacttttt tattaaatgc acttaatcct tcttgaattt
      901 gtttaggatc ttgtgtagct aatttctttt gtagttctgc ttgtgctgtt gccattgtgc
      961 tttcttgtgc cgtattccaa tcaactaatt tttggtaatc tgcttttgca gcttcgcttg
     1021 gagcggctac agctgtttca gcaggtttgg ctgcttctgc agcaggagct gttgtagttg
     1081 gtgcagcttc agttgttttt gtaggttctg tcgccggtgc tttttctgct ggtttatcac
     1141 aagctgctaa gaataatgcg actaaagcgg ttgcactgat ttttgttaat ttgttcatta
     1201 aaaaattcct caagaatata aaaataaaag ccaataaagg ctggttattg taccaataaa
     1261 tagacaagta atctccgtat tagttcataa aatcgttcat ctttacgtta tttcgaaaaa
     1321 atttacattt tataagtaaa tagcttgctg aataagctgc tttttcacaa aaggcagctg
     1381 gttggttagg cttaaaccaa ttcctctgaa taatttttta agcgggtgat tgccgtcaaa
     1441 tagctgtttt aatccttcca ttgtggctaa taatttcatc gcttgggctt tacgctcacg
     1501 ttcaaattgg cgtaaatggc ggtattcacc aatatcttgg ttgtttacca actgtttttg
     1561 gatttcgttt gccagcgttc tggcatcggc aaagcctaaa ttgacaccaa gtcccgccaa
     1621 tgggtgaatg gtatgagcgg catcaccaat caacgcaata cgaggttggg caaagtcacg
     1681 gcagaagcgt gctgcaagcg ggtaaatttc acgcaaactt tgcacttcac ataagccaag
     1741 ctgattatcg aaggcaatcg ttaaggcttt attaaattcg gcttcgcttg cattttttaa
     1801 ttcattggct ttgtttggtg gcagcgacca aacaatcgag cagagatgct catctgccat
     1861 tggcagaaaa gcaaaaatgc tatcttcagc aaagatttga cgagcggttt tgccgtgctt
     1921 ttcggttgtt ttgacattac agactaaggc ggtatgttga taatctttac tggtaagcgg
     1981 cattttcatc tggttacgca cccacgaatt tgcaccgtct gccccgacaa ccaatttagc
     2041 agacagcatt tcgccgttat ctaaggttaa aaatgccccg ttttcgctca cgcccagagt
     2101 ttggggggcg gcgataatat attctacatt agcttgttga gaaacctgtt gccacaatgc
     2161 gtgttgaatc accgcatttt ccaaaataaa gccaagttgt gaaagtccta attgcttgat
     2221 ttcattatca ttattatcaa aatggatatt ggcaaagctg tctttttccc acactttcat
     2281 ttcggtgtaa ggagaaaggc gttgagccgg aatgtgttga aaagcaccaa tctgttccag
     2341 tattttttgg cttgaagcat taatcgcact tacacggtgg taaatcgtat cggcatcaaa
     2401 tttgggcgtg tttttctcaa taattttaat ttggcaatcg acatctttga gcagtccggc
     2461 aagagccagc ccaaccatac cgccgccaac aataacaata tctgcggatt tcataaaatc
     2521 aaatgtaggg gcggtgccct gtgcccgccc taaaatagtt gaaatgaggg ctggcacagg
     2581 accagcccct acttatttaa gatgtttttt tatggtgttt ttccaaactt ttatgccgaa
     2641 cttgcacatt tttgcctttt gcttggaaat attttgccag ttgctcggca ataaatacgg
     2701 aacgatgttt tccacca
//
LOCUS       DANJWC010000211         2640 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.212, whole genome shotgun sequence.
ACCESSION   DANJWC010000211 DANJWC010000000
VERSION     DANJWC010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.212"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(269..838)
                     /locus_tag="PWX02_002290"
     CDS             complement(269..838)
                     /locus_tag="PWX02_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HDL1730258.1"
                     /translation="MNQENHKIEDIIRIFNQCFANEYNTRLERGGNYPIYLPEFMDED
                     GVPSERPYNVILFAHGYYSSALHEIAHWLVAGEARRKLEDFGYWYEPDGRSAERQREF
                     ESAEIKPQAIEWVLATAAGFRYFASADNLSGNPGDNSAFKQAVYDQVKLYAERGYLPK
                     RAETLRKALCAFYGTPDVIDLAQFDIERI"
     gene            complement(848..2611)
                     /locus_tag="PWX02_002291"
     CDS             complement(848..2611)
                     /locus_tag="PWX02_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HDL1730259.1"
                     /translation="MIRYFFSLVLSLLMINSSHANLFSQPKFLKSEEAFVFSIQNENE
                     TLQLNWDIADNYYLYKKEIRVTPQNIEIGDITFPAAEQYNDEFFGQVEIYRNHLGLKV
                     PFKEIKDNASIAVEYQGCTKGFCYPPERVELKLDSILSATPEELAQLEAQAVEKSENF
                     AKTDNMAPPENVAKAEQDQLAENLANNRFSIFWFFVLGVGLAFTPCVLPMLPLLSAIV
                     IGNKQRPSTAKALLLSIAYVQGMAVTYTLLGLIVAAIGLPFQIALQSPPVLIGLSIVF
                     ILLACSMFGLYEIQLPNSWQQKLNAMSQKQQGGALGSVFLMGMIAGLIASPCTSAPLS
                     GALLYVAQSGDLVTGGLALYLLALGMGLPLILITLFGNKILPKSGEWLLKVKAAFGFV
                     MLALPVFLLSRVLPSHYEPFLWSALAVAFLIWLVDAIPSNSITKKIIKVLLLLALAIA
                     AKPWADLAWNGSTTQQQTTPHLALEKIQSLADLETKLTASKGKKVMLDLYADWCVACK
                     EFEKYTFSDQNVQNKLNEMVVLQIDMTKNSAENIELMKHFNVLGLPTILFFDENGNEM
                     SQSRVTGFLDAEQFLAWLNKL"
BASE COUNT          791 a          536 c          554 g          759 t
ORIGIN      
        1 gctggtggaa acggtggata aagtgaaaat ctaaagtgct cataatctta tttttacttt
       61 ctatttcagt gaacagttgt acgctaatat aatagaaaat atgaaaaaaa gaaacagttg
      121 tactctgaaa caaagggttt tggataggaa gtaatcaagg caatagaaat agataagtag
      181 ggtatcgttt agtacaaatg caatattttt caaaaatgag accgcttgta tatgcaacaa
      241 gcggtctgtt ttgttaaaaa ttttgcaact aaattcgttc aatatcaaat tgagctaaat
      301 caatcacatc aggtgtaccg taaaaagcac agagggcttt acgcaaggtt tcagctcgtt
      361 tgggaaggta gccacgttct gcatagagct tgacttggtc gtacactgct tgtttaaagg
      421 ctgaattatc gccgggattg cccgagagat tgtcggcact agcaaaataa cgaaaaccgg
      481 cagcagttgc taacacccat tcaatcgctt gcggtttgat ttccgcactt tcaaattcac
      541 gttggcgttc ggcagagcgt ccgtccggct cgtaccaata gccaaaatct tcaagtttac
      601 gccgagcttc tccagcaacg agccaatggg caatttcgtg caaggcactg ctgtaatagc
      661 cgtgagcaaa taaaatcaca ttgtaagggc gttcgctcgg cacaccatct tcgtccataa
      721 attcgggcaa gtaaatcgga taattacccc cccgctctag gcgagtgtta tattcattag
      781 caaagcattg attaaaaatg cgaataatgt cttcaatttt atgattttct tgattcattt
      841 cttttcttta taacttattc agccaagcta aaaattgctc ggcatccaaa aagccagtaa
      901 cacgagattg gctcatttca ttgccgtttt catcaaaaaa cagaatcgtt ggcaaaccta
      961 atacattaaa gtgtttcatc agctcgatat tttcagccga gtttttcgtc atatcgattt
     1021 gcaacactac catttcattc agtttgttct gaacattttg gtcactaaag gtgtattttt
     1081 caaactcttt gcaggcgaca caccagtcgg catagagatc gagcatcact tttttacctt
     1141 tggaagcggt caattttgtc tctaaatcgg caagtgattg aattttttct aacgctaagt
     1201 gcggtgtagt ttgctgttga gtggttgagc cgttccaagc taaatctgcc caaggtttag
     1261 cggcaatggc taaagcgagc aataataaga ctttgatgat ctttttcgtg attgaattag
     1321 atggaatcgc atccaccaac cagatcaaaa atgcaaccgc aagggccgac cataaaaacg
     1381 gttcatagtg gcttggcagc acacggctta ataagaacac cggtaaggca agcataacaa
     1441 aaccgaatgc agctttcact tttaacagcc attcgccgga ttttggcaga attttgttgc
     1501 cgaataaggt aatcaaaatc agcggtaagc ccattcccag tgcgagtaga tagagggcta
     1561 agccaccggt gactaaatcg ccactttgtg ccacataaag caacgcaccg gaaagtgggg
     1621 cagaagtaca aggggaagca attaagcctg cgatcatccc cattaaaaac acgctaccca
     1681 acgcaccacc ttgctgtttt tggctcattg cattgagttt ttgttgccag ctatttggta
     1741 gctggatttc gtataagcca aacattgagc aggcaagcag aataaagacg atagataaac
     1801 caatcaacac cggtgggctt tgtaaggcga tttggaaagg caagccgata gccgctacaa
     1861 ttaagccaag taaggtgtaa gtaactgcca ttccttgcac ataggcaata cttaacagca
     1921 atgctttagc ggtagaaggg cgttgcttat ttccaattac aatcgcagag agaagtggta
     1981 gcatcggcag tacgcaagga gtgaatgcta atccaacgcc taatacaaaa aaccaaaaaa
     2041 tggaaaaacg attgttagct aaattttcag ccagttgatc ttgctctgct tttgccacat
     2101 tttcaggggg cgccatattg tcggtttttg caaaattttc gctcttttcg accgcttgtg
     2161 cttccagttg cgccagttct tcaggtgtag cagagagaat actgtctaat ttaagctcaa
     2221 cccgttccgg tggatagcaa aagcctttgg tacacccttg atattccact gcgattgagg
     2281 cgttgtcttt aatttcttta aacggtacct ttagccctaa gtggttgcga tagatttcca
     2341 cctgcccgaa aaattcatcg ttatattgct cggcagcggg aaaagtgatg tcgcctatct
     2401 caatattctg tggcgtgaca cggatttctt ttttataaag ataataattg tctgcaatat
     2461 cccaatttaa ctgtaatgtt tcattttcat tttggattga aaaaacaaag gcttcttccg
     2521 attttaggaa tttgggctga gagaaaagat ttgcgtgaga tgagtttatc atcaataaac
     2581 tcaagacgag tgaaaaaaag tatctaatca tattgaaatc ctatttttaa ttgtctcggc
//
LOCUS       DANJWC010000212         2562 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.213, whole genome shotgun sequence.
ACCESSION   DANJWC010000212 DANJWC010000000
VERSION     DANJWC010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.213"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..100)
                     /locus_tag="PWX02_002292"
     CDS             complement(<1..100)
                     /locus_tag="PWX02_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginyl-tRNA synthetase"
                     /protein_id="HDL1730260.1"
                     /translation="MNIQQILSDKIKQAMVASGADSNVEPLVRQSGK"
     gene            134..733
                     /locus_tag="PWX02_002293"
     CDS             134..733
                     /locus_tag="PWX02_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL1730261.1"
                     /translation="MDKIIAENAKFFEKMTACFGDLSQFEQKIRQIAEIAGIDLAAYE
                     IDHLAVRMNQTETAEQWKILLLDGATLLKESEVNGRPIGLFALIQAVEFCGQFVKIVE
                     LPFPKNKVYLEEGWEHIELVFPMLDNESSEQWVSRALSHFRLADNSEIKLKISQPQVE
                     GERLPNPSIAISLRNVTFCNPYCLKLHPYDIKSVIMSEN"
     gene            754..1218
                     /locus_tag="PWX02_002294"
     CDS             754..1218
                     /locus_tag="PWX02_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDL1730262.1"
                     /translation="MKKLALAVALMSSFALVGCANTDVFSGSVYSGEQAKEARSIAYG
                     TIVSVRDVKIQARSEGVIGTVGGGVLGGVAGNAIGGGTGQAIATAVGAVAGAVIGNQV
                     EQKASQVSSLEMVIRKDDGKEIVVVQKKEDGFVPGKRVRIVGSNSDLNVSLL"
     gene            1249..1524
                     /locus_tag="PWX02_002295"
     CDS             1249..1524
                     /locus_tag="PWX02_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDL1730263.1"
                     /translation="MKKLSLAVLASLMLAACTADVYSDKGNATVLSSKAVSNDVVELT
                     VQRDNGETVTLTREYDAHAAVGARVHLADEIKNEDSDLKTIRRYEFK"
     gene            complement(1586..2536)
                     /gene="tal"
                     /locus_tag="PWX02_002296"
     CDS             complement(1586..2536)
                     /gene="tal"
                     /locus_tag="PWX02_002296"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDL1730264.1"
                     /translation="MSQLDTLSKMTVIVADTGDIEAIKLYKPKDATTNPSLILSASAL
                     PQYAPLIEEAINYAKAQSSDKDQQLVDAEDKLAVNIGLEILKLVPGRISTEVDARFSY
                     DTEKTIAKARKLIGLYNDAGISNDRILIKIASTWQGIKAAEQLEKEGINCNLTLLFSQ
                     AQARACAEAGVYLISPFVGRILDWYKAHEGKEYAPAEDPGVISVTNIYNYYKQHGYDT
                     IVMGASFRNVGEIIELAGCDRLTISPPLLKELQESDEKLVCKLDYKGEIKPRPEKMTE
                     AEFYWEHNQEPMAVEKLAEGIRKFAIDQEKLEAMLAEKLQ"
BASE COUNT          746 a          446 c          538 g          832 t
ORIGIN      
        1 gcttgcccga ttggcggact aaaggctcga cattgctgtc tgcaccgctt gctaccattg
       61 cctgtttaat tttatctgat aaaatttgtt gaatattcac tgtttaacct atttaaattc
      121 tatttacaaa aaaatggaca aaattatagc agaaaatgca aaatttttcg agaaaatgac
      181 cgcttgtttt ggcgatttat cgcaatttga acagaaaatc cgacaaattg ctgaaatcgc
      241 cggcattgat ttagcagcgt atgaaatcga tcatcttgcg gtaagaatga accaaaccga
      301 aaccgcagaa cagtggaaga tccttttgct tgatggggca accttactta aagagagcga
      361 ggtaaacggc agaccgatag ggctgtttgc attaatacaa gcggttgaat tttgcggaca
      421 atttgtaaag attgtcgagt tgccttttcc gaaaaataag gtttatttag aagaaggctg
      481 ggaacatatt gagctggttt ttccgatgtt agacaacgaa agttcggaac aatgggtgtc
      541 aagggcatta tcgcacttta gacttgctga taattctgag attaaattaa aaatcagcca
      601 acctcaggta gaaggggaga gattgccaaa tccaagcatt gcgatcagct tgagaaatgt
      661 aactttttgt aatccttatt gtctaaagct ccacccttat gatataaaaa gtgtaattat
      721 gtcagaaaat taacgtttcg gagaacaaac aaaatgaaaa aattagcttt agctgtcgca
      781 ttaatgtcaa gttttgcttt agttggctgt gcaaatacag acgtgtttag cggaagcgtt
      841 tattccggtg agcaggcaaa agaagctcgt tctatcgctt atggcactat tgtttcagtg
      901 cgtgatgtga aaattcaagc acgcagtgaa ggtgtgatcg gtactgtcgg cggtggtgta
      961 ttaggtggcg ttgccggtaa tgcgattggc ggtggtacag ggcaagcgat tgcaaccgca
     1021 gtgggggcag ttgccggtgc ggtaattggt aatcaagtag aacaaaaagc gagtcaagtt
     1081 tcttcattgg aaatggttat tcgcaaagat gatggtaaag aaatcgtggt cgttcaaaag
     1141 aaagaagacg gttttgtgcc tggcaaacgt gtgagaatcg tagggtctaa ttcagacctg
     1201 aatgtttctc tgctttaatt ttagtttttc ctagaggata tattattaat gaaaaaactt
     1261 tctttagctg tattagcttc acttatgtta gcagcttgta ctgcagatgt ttattctgat
     1321 aaaggtaatg caacagtttt atctagcaaa gcagtatcaa acgatgttgt tgaacttaca
     1381 gttcaacgag ataatggtga aactgtgact ctaactcgtg agtatgatgc acatgcagcg
     1441 gttggtgctc gtgttcatct tgccgatgaa attaaaaatg aagatagcga tctaaaaacg
     1501 attagacgtt atgagtttaa ataatcattt atagaaatgt aaaaccggca ttaattatcg
     1561 ttaatgccgg ttttttatgc gttgtctatt gaagtttttc agcaagcatt gcttctaact
     1621 tctcttggtc aatcgcaaat ttacgaatgc cttcagctaa tttttctact gccattggct
     1681 cttggttatg ctcccagtag aattctgctt ctgtcatttt ttctggacga ggcttgattt
     1741 cacctttgta atctagcttg caaactaatt tttcatcgct ctcttgtagc tcttttaata
     1801 acggtggtga tatggttaaa cggtcgcaac cagctaactc aatgatctct cctacattac
     1861 ggaaacttgc acccattaca atagtgtcat aaccgtgttg tttgtagtaa ttgtatatat
     1921 tggttactga aattacacct ggatcttcag ctggagcata ttctttacct tcgtgagctt
     1981 tgtaccagtc taaaatacga ccaacgaatg gagagattaa gtaaacccct gcttctgcac
     2041 acgcacgagc ttgagcttga gagaataata acgttaagtt acagttaatg ccttcttttt
     2101 ctaactgttc tgctgccttt ataccttgcc aagtagaggc aattttaatt aaaatgcgat
     2161 cattactgat gcctgcatcg ttatataatc caattaattt acgtgcttta gcgatagttt
     2221 tttcagtatc ataagaaaaa cgagcatcaa cttctgtaga aatacgtcca ggtacaagtt
     2281 ttaagatttc taaaccaata tttaccgcta atttatcttc agcatcaact aactgttgat
     2341 ctttatcgct actttgtgct tttgcgtaat taatcgcttc ttcaattaat ggagcatatt
     2401 gcggtaatgc cgatgcactt aaaattaaag acgggtttgt tgttgcatct tttggcttat
     2461 aaagtttgat tgcttcaata tcaccagtgt cagcaacgat aacggtcatt ttgcttaagg
     2521 tgtcaagttg gctcatagtc tgattccttt tttgattgtg aa
//
LOCUS       DANJWC010000213         2560 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.214, whole genome shotgun sequence.
ACCESSION   DANJWC010000213 DANJWC010000000
VERSION     DANJWC010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.214"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..428
                     /locus_tag="PWX02_002297"
     CDS             <1..428
                     /locus_tag="PWX02_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730265.1"
                     /translation="FAAAEKIVTKDLPAEGKPAVVLQVFDESEKIAKPIKGNKLFISK
                     KQTRLCWHSINLPVQNKVTIAEAFYAPAAMKLASPGSKINSSADKKNHTIITEINLAN
                     NQVLNRCWGFDKTDPVGKYKMEIQINDHIFKGLEFEIVK"
     gene            complement(465..1316)
                     /locus_tag="PWX02_002298"
     CDS             complement(465..1316)
                     /locus_tag="PWX02_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDL1730266.1"
                     /translation="MENQKQAEIELKIMLEQQNVALVENYLNQTSPFPVLAHQTELLG
                     NTYYDTPEQFFATQKMGLRVRSVNQQYEITLKTQGEIVGGLHIRPEYNLALPNSQPDF
                     QTLVEKYQLPFENSEQIAESLQAIFSTDFTRKTWLVQVGMSDIEVALDQGIIKNHFGE
                     EPICELEFEIKQGNLGDLFTLVEAMPKADGMWLSNLSKAQRGYLLGQAVKWEQKIATA
                     MDGENSEKLTQLLADFICVGNESLEVLVRFNQCTQQQFSNWQQARAFVKSKDYLLHNL
                     TNLKTMI"
     gene            1377..>2560
                     /locus_tag="PWX02_002299"
     CDS             1377..>2560
                     /locus_tag="PWX02_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HDL1730267.1"
                     /translation="MFSKIHHKLNNFVQRGLDNHLRLAVTGLSRSGKTAFITSLVDQL
                     LHINKGGNSHLNLFAPARHGQILSVKRVEQGDLTIPRFEYDKNRQSLENQPPQWPEST
                     TGISEIRLAIRYQRQHSLLRHIKETGTLYLDIFDYPGEWLLDLPLLSLSYKEWSQTQQ
                     TIHKDERAELATPWLQAVKKLDLFAKTDENLLAEFSQQYTDYLLACKAQGMQYIQPGR
                     FVLPTENAKGAPVFQFFPLLDLSEAEWEKLEQSDRNSIFHTLKKRYEHYQKKIVKPFY
                     EDYFSQFDRQVILADCLTPLNYGYNEFMEMKLGLQQLFKHFHYGNRSLFHRLFSSNID
                     KLLFIATKADHITSDQVPNLESLMRQLVQEGGRHAEFDGIETGYLAISSIQATDAVKV
                     TQ"
BASE COUNT          779 a          571 c          462 g          748 t
ORIGIN      
        1 gctttgctgc cgctgaaaaa atagtcacta aagatttacc ggcagaagga aaacctgcag
       61 tcgtgctaca ggtttttgat gaaagtgaaa aaattgcaaa acctattaag ggaaataagc
      121 tctttattag caaaaaacaa acaaggcttt gctggcacag tattaatctt cctgtacaaa
      181 ataaagttac tattgcagaa gctttctatg cccctgctgc aatgaaatta gcttctcccg
      241 gctctaaaat taattcttct gcagacaaga aaaatcatac tatcattact gaaataaacc
      301 ttgcaaataa tcaagtatta aatcgttgct ggggttttga taaaactgac cctgtcggta
      361 aatacaaaat ggaaattcaa attaacgatc acattttcaa aggtttagaa tttgaaattg
      421 taaaataacc taaaagccac agagtaacac cctgtggctt tttgctaaat cattgttttt
      481 aaattggtta aattgtgcag caaataatct ttactcttca caaaggctct cgcctgttgc
      541 cagttgctaa attgttgctg agtgcattga ttaaaacgta ccagcacttc caagctttca
      601 ttgccgacac aaataaaatc agctaataat tgggttaatt tttcactgtt ttcaccatcc
      661 attgcagttg caattttctg ctcccattta accgcttgtc cgagcaaata accccgctgg
      721 gctttgctta aattactcaa ccacatccca tccgcttttg gcattgcctc aaccaaagta
      781 aataagtcgc ctagatttcc ttgcttgatt tcaaactcca attcacaaat cggctcttca
      841 ccaaaatgat ttttaatgat gccctgatct aacgctacct caatgtcaga catccccact
      901 tgcaccaacc aagttttacg agtgaaatcg gtgctgaata tcgcttgcaa gctttctgca
      961 atctgctctg aattttcaaa cggcaactgg tatttttcca ctaatgtttg aaaatccggc
     1021 tggctattgg gtaaagctaa attatattcc ggacgaatat gcaaaccgcc tacaatttcc
     1081 ccttgtgttt ttaacgtgat ttcatattgt tgattcacag aacgcacccg cagccccatt
     1141 ttttgcgtgg caaaaaattg ctcgggcgta tcataatagg tgttgcctaa caattccgtt
     1201 tgatgagcaa gcacagggaa tggcgaggtt tgattcaagt agttttccac taacgccaca
     1261 ttctgctgtt ccaacattat tttcagttcg atttctgctt gtttttggtt ttccatttca
     1321 cacgcccctg caacgttgta taatcaagcg gttattttat caataaattt tgcaaaatgt
     1381 tttcaaaaat tcaccataaa ctgaataact ttgtccagcg tggactcgac aaccatttac
     1441 gcctcgccgt taccggctta agccgtagtg gcaaaacggc atttattacc agcttggttg
     1501 atcagctttt gcatatcaac aaaggcggta attcgcattt aaacctgttt gcgcctgcac
     1561 gccacgggca aattctatcg gtaaaacgcg ttgagcaagg ggatttaact attccccgct
     1621 ttgaatatga taaaaaccgc caaagccttg aaaatcaacc acctcaatgg cctgaatcca
     1681 ccactggcat tagcgaaatt cgccttgcga ttcgctacca gcggcaacat tctctgctac
     1741 gtcatattaa agaaaccggc acgctttatt tggatatttt cgattacccg ggcgaatggc
     1801 tgttagattt acccctgctc tccctttcct ataaagaatg gtcgcaaacg caacaaacca
     1861 tacataaaga tgagcgtgca gagcttgcca caccttggtt acaagcggtt aaaaaactcg
     1921 atctttttgc aaaaaccgat gaaaatttac tggcagaatt cagccaacaa tacaccgatt
     1981 atttgctcgc ctgcaaagct caaggaatgc aatatattca gccggggcga tttgtgttgc
     2041 caactgaaaa tgccaaaggt gcaccggtat tccaattttt tccgttatta gatttaagcg
     2101 aagctgagtg ggaaaagttg gaacaaagcg acagaaacag tattttccat accctcaaaa
     2161 aacgctatga gcattaccag aaaaaaatcg tcaaaccgtt ttatgaagat tacttctctc
     2221 aatttgaccg ccaagtgatt ttggcagact gcctcacacc gttaaattat ggctataacg
     2281 aatttatgga aatgaaatta gggttacagc aactgtttaa acatttccat tatggcaatc
     2341 gttcgctatt tcaccgtctg ttttcgtcca acattgataa attgctattt atcgcaacaa
     2401 aggcagatca cattactagc gaccaagtgc cgaatttgga aagcctaatg cgacaattag
     2461 tgcaagaagg gggaagacac gcagaatttg atggtatcga aaccggctat ttagccattt
     2521 cctcgattca agcaaccgat gcggtgaaag tgactcagca
//
LOCUS       DANJWC010000214         2550 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.215, whole genome shotgun sequence.
ACCESSION   DANJWC010000214 DANJWC010000000
VERSION     DANJWC010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.215"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(160..750)
                     /locus_tag="PWX02_002300"
     CDS             complement(160..750)
                     /locus_tag="PWX02_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL1730268.1"
                     /translation="MPEFCERLQNSNLELLTRYLIQWQLLTMIRANEAVTAEYTDIDE
                     EKRLWVIPPQKMKQKHGGKPHIVPLSKQAYSLLQKIKTLANGNGYLFPSVRTKTGRMN
                     PQTVNKAIRKSLGYSKEALTAHGLRKTTSSYLHEKGILPDVVELCLSHTIGGLRGVYN
                     KAKYLPQRREAMQLWGDYVAQCMAKSGVSHLKLVAW"
     gene            complement(845..1411)
                     /locus_tag="PWX02_002301"
     CDS             complement(845..1411)
                     /locus_tag="PWX02_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HDL1730269.1"
                     /translation="MARSTTPLNSTQIDKAKPKEKEYTLADGKGLYLLVKPNGTKLWR
                     FNYYKPYTNPKKQALISIGSYPDVSLQQARQTKDEYNALLAQNIDPQAHRIETTITEQ
                     NRLVNTFAYVAEQWKASKEDVIKPLTLSKNWRIAEIYLLPHLKDLPIDKIIPAVVKPV
                     LDIPYKANKAKMFRKSMRLLNEILDYRK"
     gene            complement(1613..1698)
                     /locus_tag="PWX02_002302"
     tRNA            complement(1613..1698)
                     /locus_tag="PWX02_002302"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1662..1664),aa:Leu,seq:gag)
     gene            complement(1723..2067)
                     /gene="secG"
                     /locus_tag="PWX02_002303"
     CDS             complement(1723..2067)
                     /gene="secG"
                     /locus_tag="PWX02_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDL1730270.1"
                     /translation="MLINVLTIVYLVVAVLLIAFVLMQQGKGADAGASFGAGASATVF
                     GSAGSANFLSRTTALLATIFFAISLVIGNLSSHNVAPKSQFEDLSQFEDLSQVEQKQV
                     EPVKIENSDIPQ"
     gene            complement(2173..>2550)
                     /locus_tag="PWX02_002304"
     CDS             complement(2173..>2550)
                     /locus_tag="PWX02_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252007.1"
                     /note="decatenates replicating daughter chromosomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL1730271.1"
                     /translation="KILRETDGLGTEATRAGIIELLFKRGFLIRKGKNIHSTEAGRIL
                     IQALPDSATQPDMTAHWEMQLNQISKKETSYQQFMFDLNQQLPHLLQSPNRQVLQNLA
                     KITPLASGNKPRYFKKRTQTAEN"
BASE COUNT          666 a          476 c          522 g          886 t
ORIGIN      
        1 atttttcagt tagttataaa aaaataatag tgaaaagtgg gtaaagtggg taaaaatcga
       61 aaagtttaca gaattttcat caaggggaat tgctggcgat aaaaaagccg tcaggcgata
      121 acctaacggc attacaagcg gtcatttttg gcggaaaatt taccaggcga ctagttttaa
      181 gtgacttact ccgctttttg ccatacattg agcaacataa tctccccata actgcatagc
      241 ttcccgcctt tgtggtaaat atttagcctt gttataaacg ccccgtaatc cgcctatagt
      301 gtgagataaa cacagctcta ccacgtcagg caatatgcct ttttcgtgta gataactgct
      361 tgtagttttg cgtaagccgt gagcggtcag tgcttctttg ctatatccta aagactttct
      421 gatcgcttta ttaacggttt gcgggttcat tctgcctgtt ttcgttctta cacttgggaa
      481 taaatagccg ttgccattgg caagcgtttt gattttctgt agtagcgaat aagcctgttt
      541 tgataatggt actatatgcg gcttgcctcc gtgtttctgt ttcatttttt ggggtgggat
      601 aacccaaagc ctcttttctt cgtcaatatc tgtatattca gctgtaactg cttcatttgc
      661 tctgatcata gtaagaagtt gccattgtat taagtatcga gtaagtaact ctaaattaga
      721 gttttgtaac cgttcgcaaa attcgggcaa ttcctcaaaa ggaatataag caaattcccc
      781 tcttggtttt gcttcaaatg cttttttgat attgctacaa ggatttagcg gaatgatgaa
      841 taagctattt acggtagtct aaaatttcat taagtaacct catcgacttt ctgaacattt
      901 tagctttatt tgctttgtag ggaatatcta aaacaggctt cactactgcc ggtataattt
      961 tatctatcgg caagtctttt aagtggggga gtaagtatat ttcagcaatc cgccagttct
     1021 tagagagtgt tagcggtttt ataacgtcct ctttgcttgc cttccattgc tccgccacat
     1081 aagcgaaggt gtttactaaa cggttttgtt ctgtgatggt ggtttctata cggtgggctt
     1141 gcggatcgat attttgagca agaagagcat tatattcatc tttagtttgc cttgcttgct
     1201 gtaaagaaac atcggggtaa ctgcctatgc tgattaaagc ctgtttttta gggttagtgt
     1261 atggtttgta ataattaaac cgccacaact tagtcccgtt gggtttgaca agcagataaa
     1321 gccctttgcc gtcggctagg gtatattctt tttcttttgg cttggcttta tcaatttgag
     1381 tgctatttaa tggtgtagtt gaacgtgcca tcttttcccc ttgcgttagt tatacgctat
     1441 taagttttag ttatacgact ttttagattt gctataccgt ataactaaac gtataactaa
     1501 aatcttcgga tttgattgta ctcgataggt atgcatagga caataaaaaa gccttgaagt
     1561 attgattttc aaggctttta aggtacctat agaactctat aggtctttaa tttggtgctc
     1621 tgggcgagac ttgaactcgc acggactgtg tccaccaccc cctcaagatg gcgtgtctac
     1681 caattccacc accagagctt tattcttttt aatgttaagc gattattgag ggatatcgct
     1741 attttctatt tttacaggtt ctacttgctt ttgttcaact tggcttaagt cttcaaattg
     1801 gcttaagtct tcaaattggc ttttcggtgc tacattgtgt gagcttaaat taccgataac
     1861 taaactaatt gcgaagaaaa tcgttgcaag tagagcagtt gttcttgata aaaaatttgc
     1921 tgatcctgct gagccgaaaa ctgttgctga agcacccgcc ccgaaagaag cacctgcatc
     1981 agcgccttta ccttgttgca ttaatacaaa tgcgatcagt aatacggcaa ctacaagata
     2041 aacaatagtg agtacattaa ttaacattaa tttattctct tgtattcttt atgcgaaatt
     2101 tgcaatggcg tgcataatat agaatttacg cccgtttgac aagtgctaaa cagtaatttt
     2161 tacctgcttg atttaatttt cagcagtttg tgttcgcttt ttaaaataac gaggtttatt
     2221 gccgcttgca agcggtgtga ttttcgctaa attttgcaac acttggcggt ttgggctttg
     2281 taataaatgt gggagctgtt ggtttagatc aaacataaat tgctgatagc tggtctcttt
     2341 tttgctgatt tgattcagct gcatttccca atgagcggtc atatccggct gggtagcaga
     2401 gtcgggaagt gcttgaatca atattctgcc ggcttcggta ctgtggatat ttttcccttt
     2461 acggattaaa aaaccacgtt taaacagcaa ttcgataata ccggctcggg ttgcctccgt
     2521 gcctaaacca tcggtttcac gcaagatttt
//
LOCUS       DANJWC010000215         2522 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.216, whole genome shotgun sequence.
ACCESSION   DANJWC010000215 DANJWC010000000
VERSION     DANJWC010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.216"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            177..434
                     /locus_tag="PWX02_002305"
     CDS             177..434
                     /locus_tag="PWX02_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR domain-containing protein"
                     /protein_id="HDL1730272.1"
                     /translation="MNKIFKVIFNRTTQKMEVVSELAKSQGKAASSTDDRSSISPLVG
                     GLKGSFLVGGTALLMISAMPAEAVVSQVIMLDILQAILLTK"
     gene            650..2392
                     /locus_tag="PWX02_002306"
     CDS             650..2392
                     /locus_tag="PWX02_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
                     /protein_id="HDL1730273.1"
                     /translation="MSIAIGGLSKATDIGAVALGTSSYASGFNSFAAMRQSAARADYA
                     AAIGSSSWANATASFAIGASATALGERSFAIGSGVPQTLDQNTGEARRSIYDGNNNTL
                     AAGKDSFSIGTKAKTNGDSAFAIGTNAHSGSFKVEASNIGELGDITVADKDKVANNAF
                     ALGTNTRALSNDTVAFSTNATAQGDGSIAFGVNTNTTGTGAIAFGNASKAWKDNAISI
                     GWNATSHAEKVITIGTNSTVTKEGGMAIGDTAKSTGNNSIAVGYTSNVSNSNATAIGT
                     RANVTHEGGTAIGFEAESIANYATAIAYNSTASGESSIAAGVNSTVSGIRAAAVGYNN
                     TVSGNDTFVLGSNVTVNTVGSVILGSDSAQAKATYENTATVGKLTYSGFAGNDNVAEG
                     DYVSVGNATNARQIKFVAPGNISSTSTDAINGSQLYATNKMLDNVTNSVVNNFGGNSQ
                     VDANGNITFTNIGETGKDTIHDAIKAAKTQVKQGSNVNVSKATVDGTDVYTVNAYNTT
                     VSAVTTNGKNYLNVADNFNTGTNTTNYAVSLTDDAIRDFTKDNDTVTGVVDDGKDYKL
                     KKFHLQKMKIALTK"
BASE COUNT          809 a          387 c          545 g          781 t
ORIGIN      
        1 tgaggttttg aagtgaaaat cttactatta tcgagaggac tgtcctctta taaagagcag
       61 taaagttgtt tattcggtgg gttgtaattg ccgaattatt tacgaagtaa gcttatttct
      121 aaggttaatt ctaacgtcag aaataagctt ttttatatat ctaaaggtat aaaattatga
      181 acaaaatatt taaggttatt tttaaccgaa cgacgcaaaa aatggaagtt gtgtctgaac
      241 ttgctaaatc gcaaggaaaa gcggcatcct ccactgatga tcgtagtagt attagccctt
      301 tagtgggtgg gctaaaaggt tcttttttag taggtggaac cgcgttatta atgatttcgg
      361 caatgccggc ggaggcagtt gtttctcagg taataatgct ggatatacta caggcgattc
      421 tattaacaaa ataggggata catacgctta taactattat aatccaggta gtctttctta
      481 taacgatcct ttaaatattg acagaagtac cactgggaat ctttataatg gaacgctaca
      541 tatagaaggt attggcatag gtcgtaatac agttattcag agtagtcaag ttacttctgg
      601 ttacaataat ggtattgcta tcggtgacta ttctaaatcg acaggtggtt tgtctattgc
      661 aattggtggt ttatctaaag cgacagatat cggagcagta gctttaggta catcttctta
      721 tgcttctggg ttcaactcat tcgcagcaat gcgccaatct gctgcaagag ctgattatgc
      781 ggcagcaata ggttcttctt cttgggcaaa tgcaactgca agttttgcaa ttggtgcatc
      841 ggcaactgca ttgggtgagc gttcttttgc gattggttca ggtgtacctc agactcttga
      901 tcaaaatact ggagaagcaa gacgaagtat ttatgatggt aataataata ctttagctgc
      961 tggtaaggat tcattttcta ttggtactaa agcaaaaact aatggtgata gtgcttttgc
     1021 aattggtacc aatgcacatt ctggtagctt taaagttgag gctagtaata ttggtgaatt
     1081 aggtgacatt actgttgcag ataaagataa ggttgcaaat aacgcttttg ctttaggtac
     1141 aaatactcgt gcattatcaa acgatactgt agctttctca actaatgcaa ctgcacaggg
     1201 agatggttct attgcttttg gtgtaaatac taatacaact ggtacgggtg caattgcatt
     1261 tggtaatgca tctaaggctt ggaaagataa tgctattagt attggttgga atgcaacttc
     1321 tcatgcagaa aaagtaatta ctattggtac caattctact gtaactaaag aaggtgggat
     1381 ggcaattggt gatacagcaa aatcgactgg taataattct attgcggtag gttatacttc
     1441 aaatgttagc aactcgaatg caacagcaat tggtactcga gcgaatgtaa cccacgaagg
     1501 tggtactgcg attggttttg aagctgagtc aatagctaac tatgcaacgg cgattgctta
     1561 taactcaaca gcaagtggtg aaagctctat tgcggcaggt gtaaacagca ctgtttccgg
     1621 tattcgtgca gcagcggtag gttataacaa tactgtatcg ggtaatgaca cctttgtatt
     1681 aggttctaat gttacggtta atactgttgg ttctgtaatt ttaggctcag attccgcaca
     1741 agcaaaagcg acttatgaaa atacagcaac tgtaggtaaa ttgacttata gtggtttcgc
     1801 tggtaacgat aatgttgctg agggtgatta cgtaagtgta ggtaatgcaa caaatgctcg
     1861 ccagattaaa tttgtagcac caggtaatat ctcaagcact tcaacagatg caattaacgg
     1921 ctctcaatta tatgcaacga ataaaatgtt agacaatgtg actaattcag ttgtaaataa
     1981 ttttggcggt aattcacaag ttgatgcaaa tggtaatatc acatttacaa atattggtga
     2041 aacaggtaaa gatactattc atgatgcaat caaagcagct aaaacccaag ttaaacaggg
     2101 ttctaatgta aatgtttcta aagcaacagt tgatggtaca gatgtatata ctgtcaatgc
     2161 atataacact actgtaagtg ctgttacaac gaatggtaaa aactacttaa atgtagctga
     2221 taattttaat actggaacaa atactacaaa ttatgcggtg agtttaacag acgatgcgat
     2281 tcgtgatttc actaaagata atgatactgt tactggtgta gttgatgatg gtaaagatta
     2341 taagttgaaa aaattccatc tccagaagat gaaaatcgca cttacaaagt aagcttaagt
     2401 gataaggcaa ttaatgagtt cacaaaagat acttctgcaa ctgtgaaagc tggttctaat
     2461 agaattaatg taaacggggc agctaataca actaatccag atattatgga ttatacggtt
     2521 ga
//
LOCUS       DANJWC010000216         2467 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.217, whole genome shotgun sequence.
ACCESSION   DANJWC010000216 DANJWC010000000
VERSION     DANJWC010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.217"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            57..1124
                     /gene="hisC"
                     /locus_tag="PWX02_002307"
     CDS             57..1124
                     /gene="hisC"
                     /locus_tag="PWX02_002307"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDL1730274.1"
                     /translation="MTISQLSRKNIQALTPYQSARRLGGSGDVWLNANEYALSPNFDL
                     TDRTFNRYPEPQPQAVIEGYARYAGVAPENVLVSRGGDESIELIIRAFCEANDSILYC
                     PPTYGMYAVSADTCGIATKTVPLTADFQLDLAQIKANLEGVKVVFVCSPNNPTGNLIK
                     RSDLFELLQITAGKAIVVVDEAYIEFCPEASLVSELANFPHLAIIRTLSKAFALAGLR
                     CGFTLANAELIGVLQKVIAPYPLPVPVSDIAAQALSPQGIEQMKTRVAEVITLRAELQ
                     KNLENLPLVEQVFDSEANYLLFKCQDGQKVFKALWEQGIILRDQHKALGLQNCIRITV
                     GAREENLRVVEAITALSQFQR"
     gene            1303..2391
                     /gene="hisB"
                     /locus_tag="PWX02_002308"
     CDS             1303..2391
                     /gene="hisB"
                     /locus_tag="PWX02_002308"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018355873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HDL1730275.1"
                     /translation="MQPTLFIDRDGTLIDEPKTDFQIDSLEKLKFEKNVIPALLKLKG
                     KYRFVMVTNQDGLGTSSFPQADFDKPHNAMMALFNSQGIEFDDVLICPHKPEDGCDCR
                     KPHTKLLQKYIDRQLFDPANSFVIGDRATDVQLAENLGIRALQYHPEKLNWDLIAEKL
                     LGEAVTNIGERPKRFAEVIRKTKETDIKVQVWLDETGNNEISTGVGFFDHMLDQIATH
                     GGFRMNITTKGDLWIDEHHTVEDTALALGTALKQAIGDKRGIARFGFVLPMDESRAEC
                     AMDLSGRPFIKFKADFKRDKVGDFSTELTEHFFQSIAFTMLATLHIKAKGDNDHHKIE
                     SLFKVFGRTLRQCIRIEGNELPSSKGVL"
BASE COUNT          697 a          620 c          592 g          558 t
ORIGIN      
        1 attattcaat atcgaaaaaa tttctaaact atgtataaat tatacaggaa cacaatatga
       61 caatttcaca actttcccgt aaaaacattc aagctctcac cccttaccaa tcggcacggc
      121 gtttgggggg tagtggcgat gtgtggctga atgccaatga atatgccctt tcgccaaact
      181 ttgatttaac cgaccgcact tttaaccgct accctgaacc tcaaccgcag gcggtgattg
      241 aaggttacgc ccgatatgca ggcgttgcac ctgaaaacgt gctggtttcc cgtgggggcg
      301 atgagagcat tgagctgatt atccgagcct tttgcgaggc aaacgacagc attctctact
      361 gcccaccaac ctacggaatg tacgccgtga gtgccgacac ctgtggcatt gcgaccaaaa
      421 ctgtgccttt aaccgccgat ttccagttgg atttagccca aatcaaagct aacctcgagg
      481 gcgtgaaagt ggtgtttgtg tgcagcccga acaacccgac aggcaactta atcaagcggt
      541 cggatttgtt cgaacttttg caaatcaccg caggcaaagc gattgtggtg gtggacgaag
      601 cctacatcga attttgccct gaagcgagct tggtaagcga gctggcgaat ttcccacatt
      661 tggcgattat ccgcacccta tccaaagcct ttgccttggc gggtttgcgt tgtggcttta
      721 ccttggcaaa cgctgagctg atcggcgtgt tacaaaaagt gattgccccg tatccgctac
      781 ctgtgcctgt ttccgacatc gccgcccaag ccctctcccc acaaggcatt gagcagatga
      841 aaacccgtgt agcggaagtc atcaccctgc gtgccgaatt gcaaaaaaat ctggaaaact
      901 taccgcttgt agaacaagtt tttgacagcg aagccaacta cctcctattc aaatgccaag
      961 acggacaaaa agtcttcaaa gcactttggg agcaaggcat tattttgcgt gaccaacaca
     1021 aagcgttagg attgcaaaat tgcattcgga ttacggtggg ggcgagagag gagaatttga
     1081 gggtggttga ggcgattaca gcactgagcc aatttcagag atagaattta acgagcagta
     1141 ttttaagaga catttatagc ctagcacggc atagccgtac taggttacgc ataagtcagt
     1201 ccctttggga ctgaatacag agaggcaaag tctctcacta atgcttaacc ccatacgcct
     1261 tggcgtgtgg ggagagtaat gaataagata caggagaaac caatgcaacc cacccttttt
     1321 atcgaccgtg acggcacgtt aattgatgag ccaaaaaccg attttcaaat cgacagcttg
     1381 gaaaagctga aattcgagaa aaacgtgatt cctgccctgc tcaaactcaa gggcaaatac
     1441 cgctttgtga tggttacgaa ccaagacggc ttaggcacga gttcgttccc acaggcagat
     1501 ttcgacaaac cgcacaatgc aatgatggcg ttgttcaact cgcaagggat tgaatttgat
     1561 gatgtgctga tttgtccaca caagcccgaa gacggttgcg attgccgcaa accgcacacc
     1621 aaactgctac aaaaatatat cgaccgccaa ctgttcgacc cagccaacag ctttgtgatt
     1681 ggcgaccgtg ctaccgatgt gcaactggcg gaaaaccttg gcattcgggc gttgcaatat
     1741 caccctgaaa aactgaactg ggatttgatt gctgaaaaac tactgggcga agccgtcacc
     1801 aacattggcg agcgaccaaa gcgctttgcc gaagttatcc gtaaaaccaa agagaccgac
     1861 atcaaagtgc aagtgtggct ggacgaaaca ggcaacaacg aaatcagcac cggcgtgggc
     1921 tttttcgacc atatgcttga ccaaattgcc acccacggcg gttttagaat gaacatcacc
     1981 accaaaggcg acttgtggat tgacgaacat cacacggtgg aagacaccgc cctcgccctc
     2041 ggcaccgcat taaaacaagc gattggcgac aaacgcggca tcgcccgttt cggcttcgtg
     2101 ttgccgatgg acgaaagccg tgcggaatgt gcgatggact tatcaggcag acctttcatc
     2161 aagttcaaag cggatttcaa acgcgacaaa gtgggcgatt tcagcaccga gctcaccgaa
     2221 cacttcttcc aatccatcgc cttcacgatg ctcgccaccc tgcacatcaa ggcaaaaggc
     2281 gacaacgacc accacaaaat cgaaagcctg ttcaaagtct tcggcagaac actcagacag
     2341 tgcattcgta ttgaagggaa tgaactacca agcagtaaag gtgtattata atcaccgtag
     2401 gggcgggttt taacccgccc gaaagtgcgg ttgaaatttg caaagttttt ggggattttg
     2461 accgctt
//
LOCUS       DANJWC010000217         2451 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.218, whole genome shotgun sequence.
ACCESSION   DANJWC010000217 DANJWC010000000
VERSION     DANJWC010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.218"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(232..>2451)
                     /locus_tag="PWX02_002309"
     CDS             complement(232..>2451)
                     /locus_tag="PWX02_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDL1730276.1"
                     /translation="DIVYDKTIAIDSSIKEVIKTIGEATVIVLVVIILFLGSLRAMIV
                     PVITIPISLIGVLFFLQMFGFSINLLTLLALVLAIGLVVDDAIVVLENVERHVKEGKT
                     PFDAAIIGTREIALPVISMTITLGAVYSPMALMTGVTGTLFKEFALTLAGAVFISGIA
                     ALTLSPMMSSRILKEHKEGEESAFARVVHRSLDKMTSCYTNMLSGIMAVRGFVLFFAV
                     LIFASLPMLFGSLSSEVAPTEDRGFVVGISNGPSNTNLDYTEAALAQFNQEVTKLPEV
                     SSLMTVSGFNGANSALSIIALKDWNDRSRERAAISNDVAAIGKKVVGMDVNAIAFPEI
                     STGENGLPFSLVITTADSYEKLAIVASEFLKKAQTSGQFFFANLDLKFDTVTMNMKFD
                     REKMGAYGVTMQQVSGTLGAFLSGAIITRVDIDSRAYPIVSQAVRNDRLNPEDLMNYY
                     VTTATGQSIPLGSFVTMELKTSPSSLPRMSQLNSATIGGVVSGSIGDAVNWAKAELDR
                     TLPSGYQYDFRGESRQFVQEGNAMAMTFALAVIIIYLVLAIQFESWRDPLVILVSVPL
                     AVSGALLVMNIPTAMKGLFMGIAAGTGSKDMMAFANGIWTAGYTLNIYSQVGLITLVG
                     LITKHGILMCEVAKEEQLLHGKSRYEAIFHAATIRLRPIMMTTAAMIAGLIPLLFAVG
                     AGAIARFSMGMVIVAGLAIGTLFTLFILPVIYTFLGQEHKPLREFDEEKYFPTTKEA"
BASE COUNT          770 a          549 c          466 g          666 t
ORIGIN      
        1 gcattgcaat tattccttaa aaaatgatta aattgaaaat ctgtactgga aaattgcgag
       61 cggattatac agaggaacta ggtttctgta aatagggaaa cgtttacgct atctgaaaat
      121 atactttcaa gcggttaaat tttgtgattt tttagcaaac aataaataaa aaacgaccgc
      181 ttgtaagcca acaaaaaaga gcgtatcgct acgctctttt gctatttaaa actatgcttc
      241 ttttgtcgtt gggaaatatt tttcctcgtc aaattcacgt aatggcttgt gttcttgccc
      301 taagaaagtg tagataaccg gcaagataaa cagagtaaac aatgtcccga ttgccaagcc
      361 tgctacgatt accataccca tactgaagcg ggcgattgca ccggcaccca ccgcaaacag
      421 aagcggaatt aagcctgcaa tcattgctgc cgttgtcatc ataattgggc gtaaacgaat
      481 cgtggctgcg tggaaaatag cttcatagcg tgatttgccg tgcagtaact gctcttcttt
      541 cgccacttca cacatcaaga taccgtgttt agtaattaag ccgacaaggg taattagacc
      601 tacttgtgaa tagatattta aggtataacc tgccgtccaa ataccgttgg cgaatgccat
      661 catatctttc gagccagtgc ctgctgcaat ccccataaac aagcctttca ttgcggttgg
      721 aatgttcatt accaacaatg caccactcac agctaacggc acagaaacca aaattactaa
      781 tgggtcacgc caagattcaa attgaattgc aagtacaaga tagataatga taactgctag
      841 agcgaaagtc attgccatcg cattaccttc ttgaacgaac tggcgagatt caccacggaa
      901 gtcatattga taacctgatg gtaaagtgcg atctaattct gctttcgccc aatttaccgc
      961 atcgccgatt gagccagata ctaccccacc aatggtggcc gagtttaatt ggctcatacg
     1021 aggtaaagaa gatggtgatg tttttaattc catcgtcaca aatgaaccta acgggataga
     1081 ttgccctgtt gcagtcgtga catagtaatt cattaaatct tcaggattta aacgatcatt
     1141 acgcaccgct tgcgacacaa tcgggtaagc acgagagtca atatcgacac gagtaataat
     1201 cgcaccggat aagaatgccc ctaatgtgcc actcacctgc tgcattgtca cgccataagc
     1261 acccattttt tcacggtcga atttcatatt catcgtgacc gtatcaaatt tcaaatctaa
     1321 attcgcaaag aagaactgac cggaagtttg ggcttttttc aagaattcag aggcaacgat
     1381 tgccaatttt tcatagctat cagcggtagt aatcactaat gagaatggta gaccattttc
     1441 accggttgaa atttcaggga atgcaattgc attcacgtcc atacccacca cttttttacc
     1501 gattgcagcc acatcattag aaatcgcagc acgttcacgg ctacgatcat tccaatcttt
     1561 taatgcaata atcgataatg cactatttgc accattaaag cctgaaactg tcattaagga
     1621 agatacttca ggtaatttag ttacttcctg attaaattgg gctaacgctg cttcggtata
     1681 atctaaattg gtgtttgaag gtccattaga aataccaacc acaaagccac ggtcttctgt
     1741 cggtgcaact tcactggaaa gtgagccaaa taacatcggt aaactggcga agatcagcac
     1801 cgcaaagaat aagacgaaac cacgaacagc cataataccg cttagcatat tggtatagca
     1861 gctggtcatt ttatctagtg atctatgcac cacgcgggca aatgctgact cttccccttc
     1921 tttatgttct tttaatattc ggctagacat catcggcgaa agggttaatg ccgcaatccc
     1981 cgaaataaat accgcaccgg caagcgttaa agcgaactct ttaaatagtg tccctgttac
     2041 cccggtcatt aatgccattg gtgaatatac tgcacccaga gtaatagtca ttgaaataac
     2101 cggtaaagca atttcacgtg taccgataat cgctgcatca aatggcgttt taccttcttt
     2161 aacgtgacgt tctacgtttt caagtacgac aatcgcatcg tccactacca agccgatcgc
     2221 taataccaac gccagcaaag taagcaagtt gatcgagaaa ccaaacattt gcaggaagaa
     2281 taataccccg attaaggaaa tcggaatggt aatcaccggt acaatcattg ctcgtaatga
     2341 acctaagaac aggataatta ctaccaatac aatgacggtt gcctcaccaa tagttttaat
     2401 tacttctttg atagaactat caatcgcaat ggttttatcg taaacaatat c
//
LOCUS       DANJWC010000218         2446 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.219, whole genome shotgun sequence.
ACCESSION   DANJWC010000218 DANJWC010000000
VERSION     DANJWC010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.219"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..917)
                     /gene="lpxB"
                     /locus_tag="PWX02_002310"
     CDS             complement(<1..917)
                     /gene="lpxB"
                     /locus_tag="PWX02_002310"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HDL1730277.1"
                     /translation="MKQQFPVIGLVAGEVSGDILGAGLIKALKVHYPNARFIGVTGSR
                     MIEAGCQSLFDMEELSVMGLAEVVKHLPRLLKRRKQVIDEMLKLKPDVFIGIDAPDFN
                     LTVEEKLKASGIKTIHYVSPSVWAWRQKRVFKIARAANLVLAFLPFEKAFYDKFNVPC
                     RFIGHTMADAIPLEPNRTEACLALNIDESKRYMAILVGSRGSEIQFLAEPFLKAAQLL
                     KARFPDLQFLVPMVSQKRMEQFNAIKQQVAPELELNIIQGKARQAMIASECTLLASGT
                     AALEAMLCKSPMVVGYRMKPLTYWLAKKLV"
     gene            1071..2144
                     /locus_tag="PWX02_002311"
                     /pseudo
     CDS             1071..2144
                     /locus_tag="PWX02_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011978718.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT          821 a          519 c          432 g          674 t
ORIGIN      
        1 actaattttt ttgctaacca ataagtcagc ggtttcattc tataaccaac taccatcgga
       61 gatttgcaca acattgcctc tagtgctgct gttcccgatg ccaaaagagt acattctgac
      121 gcaatcatag cctgacgagc cttgccttga ataatattca gttccaactc gggagctact
      181 tgctgtttaa ttgcattaaa ttgctccatt cgcttttggc tgaccattgg cactaaaaac
      241 tgtaaatccg gaaaacgggc ttttaacaac tgagcagcct tcaaaaaagg ctcagccaaa
      301 aactgaattt cactgccacg gcttcccacc aaaattgcca tatatcgctt ggattcatca
      361 atatttaacg ctaaacatgc ctctgtacga tttggttcga gtgggatcgc atctgccatc
      421 gtatgcccga taaaacgaca aggcacattg aatttatcat agaacgcttt ctcaaatggc
      481 agaaatgcca acactaaatt agctgctctg gcaattttaa acacccgctt ttgtcgccaa
      541 gcccacaccg aagggctaac ataatggatt gttttaatcc cactcgcttt gagtttttct
      601 tcaacggtta aattaaaatc cggtgcatca ataccgataa atacatctgg tttcaattta
      661 agcatctcat caataacctg tttacgacgc ttaagtaaac gaggtaaatg tttaaccacc
      721 tctgctagcc ccattacaga aagctcttcc atatcaaaca aagactggca gccagcctca
      781 atcattcgag agcctgtaac accgataaat cgagcattcg ggtaatgtac ttttaacgcc
      841 ttaattaacc cggcaccaag aatatcgcca gagacctcac cagcaactaa gccaataacg
      901 gggaattgtt gtttcatatt aaatattatt gaagatctgt aagtgactct attataacca
      961 atcatcatat aaactaacaa aacattattg tctagcaatg aatattcaca tacactacct
     1021 ccctttatct attcatatgg agtgattttt atgaaaaaac aacatgagcg attttagcat
     1081 cagccttaat cgcaaactca gctctagcta ttacgattta cgatcataaa gaaaccggta
     1141 cacaaattga atttattggt tctgctcgta ttaagtggga aagcacctca gataaaacct
     1201 caccggtaaa tggtaaagct acccgcaacc acgttaatca cgcagtggat aacaacggct
     1261 ctcgttttgg tttccgttta acccagcaag tgggtcacgg attctatgct ttaggtcgtg
     1321 tggaatggcg tatgcgtggc gattcaagct cacaacataa cttcgatcat atttatgatc
     1381 atcaactcta tgcaggaatc gggcataaac aatacggtga attaacatac ggcaatatga
     1441 ctaatattgc cgatgaagta aaacaaactg acttaccaaa tacactcagc ttaagtgatg
     1501 gcttacttac cggttcatca cgacgtacgc tttaatatgt ttacaaaggc atcgaaggat
     1561 taaaactcgg agcattctat ggcggaaata gccaacgtgg aaacaatggc ttagatttag
     1621 ccaataaacg taaagatatt tggggtgcag cagcaattta taactataaa attgatgata
     1681 accaaagctt aaaattagca acaggtgcaa cccgtgagcg ttctgagcaa tctatcggca
     1741 attatgaacg cactgctctt gccttcggta cagcttatac ctacggaaaa actacccttg
     1801 gcttagattt agaacgccgt gaaacggaag atcaaaatgc tatcggtcac aaacgtatcg
     1861 aaaaagaagt tcgcactgtg ttactccatc gtttaaccga tcaatggaat gcttacacaa
     1921 tgtatgctta taaagaaaac aaacttaatg ctgtaggctt ggcaaatgat agcaaagtta
     1981 aaagaaacca atttatgttg ggaacggaat attacattgt tccaaaacac ttaaaaggct
     2041 ttgttgaatg gcaagcaact cgtgctaaac actatactaa tgatgtaaaa accgcaaaat
     2101 ctcgcaatta tacgactgtt gtaggcttac gagcatattg gtaataaaac cgaaaagcgt
     2161 actagaatga tctgcaccta aaagttgggc taaccaactg attaaggtgc agatttttta
     2221 tgattaaata caactaatcc ttcaaacaag aagctattgc atttatcttc aaagtggtaa
     2281 aaaccgctta ctcacctatt agcatcttca gctttaagaa acaacactaa aattttagtg
     2341 tgaactaata taatcataat ggcaataact gagcagccaa aacgcctaaa tttaaattat
     2401 ctgttgcaaa agaggttaaa ctgcaaaaag tgatgagggg ttatta
//
LOCUS       DANJWC010000219         2400 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.220, whole genome shotgun sequence.
ACCESSION   DANJWC010000219 DANJWC010000000
VERSION     DANJWC010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.220"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..590)
                     /locus_tag="PWX02_002312"
     CDS             complement(<1..590)
                     /locus_tag="PWX02_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL1730278.1"
                     /translation="MLIEWRNQIVGDKIKAVTWNNYVRHLKALYNFGISQGLLNINRN
                     PFNQLFIRESKRRKKTYSTEQLHKIDHILNNNVVLPDFLKPSWFVLAIIMTLRCTGIR
                     RGQLVKLKISDVDLSNRTIYISPEINKNHDYHIVPISDSLYIHIERLITELKIRKQTD
                     NCQLFNLNLFSRCSRRRGQPMSQNQLTHIFRHISDLV"
     gene            complement(746..1066)
                     /locus_tag="PWX02_002313"
                     /pseudo
     CDS             complement(746..1066)
                     /locus_tag="PWX02_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248924.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
     gene            complement(1182..1970)
                     /locus_tag="PWX02_002314"
     CDS             complement(1182..1970)
                     /locus_tag="PWX02_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730279.1"
                     /translation="MFLSHFKQVLNANIEADGKDWTIIDVFGGSGLLAHTAKREKPLA
                     RVIYNDFDNYAERLNHIKDTNQLRQEIYQIVDGVIPKNKRISNEVKAKIINKINDFEG
                     FKDPKCLSSWLLFSGEQVATLDELFKHDFWNCVRQSNYPEATGYLDDIEVVSESFHQL
                     LPRFHDKEKVLLILDPPYLCTRQESYKQANYFDLIDFLRLIDLTKPPYIFFSSTKSEF
                     IRFIHYMVQNKKENWQAFEGAERIVVNASPSYNGKYEDNMVFKF"
     gene            complement(2091..>2400)
                     /locus_tag="PWX02_002315"
     CDS             complement(2091..>2400)
                     /locus_tag="PWX02_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HDL1730280.1"
                     /translation="VWYECYSSSPRDGGVRQTVINLDDGKWDLIELPNVHEQQIKAYF
                     AGTKGKPYDWRGVFGIVLGIKQKRDKYFCSEWCFNLIKQSDEGWRFSPNQLVAIFKKG
                     "
BASE COUNT          731 a          462 c          398 g          809 t
ORIGIN      
        1 accaagtcag aaatatgccg aaatatatgc gtaagctgat tttggctcat cggctgccct
       61 cttcttcgag agcatcgaga aaataaattg agattgaaca attggcaatt atctgtttgt
      121 tttcttattt ttaattcagt aataagcctt tctatatgaa tataaagtga atctgaaatc
      181 ggtacgatat ggtaatcatg atttttattg atttccggcg aaatataaat cgtcctatta
      241 gaaagatcta catcactaat tttcagtttc actaattgtc ctcgccgaat ccccgtacat
      301 ctcaatgtca taataattgc aaggacaaac cagcttggtt ttagaaagtc aggtaataca
      361 acgttattat tgagtatgtg gtctatttta tgtaattgtt cggttgaata agttttcttt
      421 ctccttttcg attctcgaat aaataactga ttaaatggat ttcgatttat attaagtaat
      481 ccttgcgata taccaaagtt atataaggct tttaaatgtc tgacataatt attccacgtt
      541 actgctttga ttttatcgcc aacaatttga ttacgccatt caatgagcat atcggtggta
      601 atgtcattgg catttaactt tggataacgc tttttgataa ttcggattac gttgttgtag
      661 ctccgtttgg tatccggacg aagatagtga cgatagaaaa aaaaatcggt ctagtagcga
      721 atcaaaagag atagaagtta tcattttaag tttcctcttt ctgtaaagtt aaacagtgat
      781 tatttaatag taccttatcc ccaaaaaaga gacgggtatt ttttatcaaa tatcctgtta
      841 aagaggaagt ctttctcggt ccaatgacat cacatttcca aaaattgatg gtatccggct
      901 taccgttggt cattgtgttt ttaggtgaat gaatccctaa tccttgaaaa tcatactgga
      961 gattattaat tgtttcctta tcataaggct gtccagtctc ttgcaaatac atttcaaaaa
     1021 taacgggggt aactaaaaac aggtggtctt caacaatgta agcggtcgaa ttattttgca
     1081 tagaattaca ctcaattttg catagataaa aattaaaaac tatgcaaaat aaatcgcaaa
     1141 aacaaacgcc ctttaaacca taattaaggg cgtttaaatt tttagaactt aaaaaccata
     1201 ttatcctcat atttgccgtt ataacttggc gaggcattta ctactattct ttcagccccc
     1261 tcaaacgctt gccagttttc ttttttgttt tgaaccatat agtgaataaa gcgaataaac
     1321 tcgctcttgg ttgagctaaa gaagatataa ggcggtttgg taaggtctat caatcgtagg
     1381 aaatcaatta aatcaaagta atttgcctgc ttataactct cctgtcttgt gcaaagataa
     1441 ggcgggtcaa gaatcaataa tactttctct ttgtcgtgaa acctcggcaa taattgatga
     1501 aaggactcac taaccacctc aatatcatca agataacccg ttgcttcagg atagttactt
     1561 tgccgcacac aattccagaa atcgtgctta aataactcat ctaaagtagc aacttgctca
     1621 ccactaaata acaaccaaga acttaagcat ttggggtctt taaacccctc aaaatcattg
     1681 attttattga tgattttagc tttaacttca ttgctaattc tcttattttt tggtataact
     1741 ccatcaacaa tttggtagat ttcttggcgg agttggttag tgtctttgat atggtttagc
     1801 cgttcggcgt aattatcaaa atcattataa atcactcttg ctaacggttt ttcacgcttt
     1861 gcagtatgtg caagcaaccc actcccacca aaaacatcta taatagtcca atctttcccg
     1921 tctgcttcaa tatttgcatt taacacctgt ttgaaatggg ataaaaacat ccgcttttga
     1981 ccgataaaag gtagcggagc ttgtttaaat attacatttg cttgctgttt agtttgtact
     2041 tgcgatgcca tcgcagcaac agctaatgcg gttaaagctt tctgcatttt ttatcctttt
     2101 ttgaaaattg caaccaactg attagggcta aatctccatc cctcatcgct ttgcttaatt
     2161 aagttaaagc accactccga acaaaagtac ttatcccgct tttgtttaat ccccaataca
     2221 atcccaaaca cgcctcgcca gtcataaggc ttgccttttg tgccggcgaa ataggcttta
     2281 atttgttgct cgtgtacgtt tggcagctca attaaatccc atttgccatc gtcaaggtta
     2341 atcacagttt gtcttactcc accgtcacga ggactggagc tgtagcactc ataccacact
//
LOCUS       DANJWC010000220         2237 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.221, whole genome shotgun sequence.
ACCESSION   DANJWC010000220 DANJWC010000000
VERSION     DANJWC010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.221"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(205..1197)
                     /gene="mglB"
                     /locus_tag="PWX02_002316"
     CDS             complement(205..1197)
                     /gene="mglB"
                     /locus_tag="PWX02_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDL1730281.1"
                     /translation="MKRSLLNAFVYTLMLGANASYAQSEPRIGVTIYKYDDNFMMLMR
                     KEMNKEMANFSHLKWFMNDAQNSQVKQLKQIETLLKHQVNVLAVNIVHANESKTIVEK
                     AKLSNVPVVFFNRSSDNQALESYDKAYFVSSDPKEAGRIQGELIAKVWKSNPDFDLNK
                     DGKLQFVLLKGEPSDVSAERSQAVVESLNQQGVQAEEIYSDTARWRRTLARNKMNEWL
                     VENRAAEIEVVISNNDEMAIGALDALNAHEKRLPIFGIDALPETLALMKKGEIAGTVL
                     NDSSAQAKAVVKLVSNLAQGKPALKDTQWESHSSKTIYLPHIGIDKENLGQFLP"
     gene            complement(1302..1705)
                     /locus_tag="PWX02_002317"
                     /pseudo
     CDS             complement(1302..1705)
                     /locus_tag="PWX02_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612952.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PACE efflux transporter"
     gene            1824..2111
                     /locus_tag="PWX02_002318"
     CDS             1824..2111
                     /locus_tag="PWX02_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDL1730282.1"
                     /translation="MKNDKPIECEGCNTFDVGSILDNSETVANITRTYETEAEAQAAL
                     EIFTQKARDVESEPCEIESEIRPVDGGVKLNANFKFSCQAEVVIFQLGTRN"
BASE COUNT          714 a          488 c          362 g          673 t
ORIGIN      
        1 aactaatttt aaaaaaacaa tccgtgatca ttctcacaga attaagacaa agtggagaaa
       61 tttttaattt gaagtataaa atttgaatat tcgctctatt caaatctgtt ggaagaaaag
      121 agaaaggatt tgcaagattt tactgaaaaa tgaccgattg ttatcatcaa taaggggcgt
      181 atttataacg ccccgctcct ccatttaagg taaaaattgc cctaaatttt ccttatcaat
      241 accaatatgt ggaagataaa tcgttttaga ggaatggctc tcccactgcg tatctttcag
      301 agcaggctta ccttgtgcca aattagatac caatttcacc accgctttcg cttgtgcaga
      361 gctatcattt aataccgtgc cggctatttc cccttttttc attaatgcta acgtctcagg
      421 caaggcatca ataccaaaga tcggcaatct tttctcgtgt gcatttaacg catctaaagc
      481 accaattgcc atttcatcat tgtttgaaat cacaacttca atctcagctg cacggttttc
      541 caccagccac tcattcattt tattgcgagc caatgttcta cgccatctcg ccgtgtcact
      601 gtaaatttct tctgcctgaa caccttgttg attaagcgat tcaaccacag cttgagaacg
      661 ctcagcgctc acatcactcg gctcgccctt aagcaataca aattgcaatt tcccgtcttt
      721 atttagatcg aaatcggggt ttgatttcca cactttcgca attaattccc cttggattct
      781 accggcctct tttggatcac tactcacaaa ataggctttg tcataactct ctaatgcttg
      841 gttatcagac gagcggttaa aaaacaccac aggcacatta gacaatttgg ctttctctac
      901 aattgttttc gattcattcg cgtgaacaat attgactgcc aatacattaa cttgatgctt
      961 aagcaacgtt tcaatctgct ttaactgttt tacctgcgaa ttttgagcat cgttcataaa
     1021 ccatttgagg tggctgaaat tcgccatttc cttgttcatt tctttacgca tcaacatcat
     1081 aaagttgtca tcatacttat aaatcgtaac gccaattcta ggttcagatt gagcatagct
     1141 cgcattcgcc ccaagcatta gcgtatagac aaaagcattt aataggctac gtttcatcgt
     1201 tttcatagaa tgtccctctt cctatgttga aatacgttag cgaaagcata gcgaatttag
     1261 caaagcgaaa ccgtgatcgg cttcacagat tagaaagatt ttcattggtt cgatgtaata
     1321 aatttcaggc gtaaatggtc ataaatccaa ttaaaaataa tggaataaac cagcacaaat
     1381 aatgtcatac caatatccat tacaaaggct tctcaccagc caatattaag catatacatt
     1441 accaatggaa tggtaaaaac caacagcccg ccttcaaata agaccgaatg cccaatcttc
     1501 ttgcaattcg ctcgcccttg actactttat caaacagcca gttatagaca aaattccaca
     1561 cccttgcgac aacagagatc ataataatca cactcgctaa cgagcttgtg ctatgttccg
     1621 tgagcagtga tgttaaaatc acggtaaaca caatggcaaa aaactcaaat aaaactgcat
     1681 gtagcacacg ctcccaagcg gtcatttttc ctccaaaatt tgcaaaaggc tatctaaatc
     1741 aaattatcgt gaaatcacga ttgcacccac gcttatttag ttttaaagta gtgatattat
     1801 ttatcaatta tgaggattag ccaatgaaaa acgataaacc cattgaatgc gaaggctgca
     1861 atacctttga tgtcggctca attttagata atagtgaaac tgttgcaaac attactcgca
     1921 cttacgaaac cgaagccgaa gctcaagcag cattagaaat atttactcaa aaagcacgtg
     1981 atgttgaatc cgagccgtgc gaaattgaga gcgaaatccg ccctgtggac ggcggcgtta
     2041 aactcaatgc taattttaaa tttagctgcc aagctgaagt agttatcttc caattaggta
     2101 caagaaatta atagaaaggc tggtttaacc agcctttctt ctctttaaaa gcactccatt
     2161 tcactccaaa gtaacaataa tgtaaaaaat tgagatatat gtcatatttt taaaaatttg
     2221 ttgtttactt ttagtaa
//
LOCUS       DANJWC010000221         2229 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.222, whole genome shotgun sequence.
ACCESSION   DANJWC010000221 DANJWC010000000
VERSION     DANJWC010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.222"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..691)
                     /gene="rapZ"
                     /locus_tag="PWX02_002319"
     CDS             complement(<1..691)
                     /gene="rapZ"
                     /locus_tag="PWX02_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDL1730283.1"
                     /translation="MELIIISGRSGSGKSVALRALEDAGYYCVDNIPLPLIPTLTYYL
                     TQENRSAVISLDIRNLPNTPNAIEELLTQLEPLNAKLIFLDCERNTLIRRYSDSRRIH
                     PLSTQDDLSLEGALDLEQKLLEPLVQNANYIIDTSHLSSHELAENLRQILQGSTEKEL
                     KIIFQSFGFKYGLPADSDYVFDVRFLPNPHWNPELRPMTGLEQPVIDFLERQTEVHNF
                     IYQTRNYLEMWL"
     gene            complement(704..1216)
                     /locus_tag="PWX02_002320"
     CDS             complement(704..1216)
                     /locus_tag="PWX02_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1730284.1"
                     /translation="MTKLTEFLSPENVRQGVLISSKKRALELVGKIVAESINQTEQQS
                     EALCPIECFGNLFKREKLGTTGLNQGIALPHAKLPACGDIELTKPIAVFLQLEEAIDY
                     EAQDNKEVDLIYAMMFPENSCEQYKSCLPAIAQKLTDKSLLKQLRAAESAEDIWQILV
                     YADNHLKDEE"
     gene            complement(1258..1983)
                     /gene="lptB"
                     /locus_tag="PWX02_002321"
     CDS             complement(1258..1983)
                     /gene="lptB"
                     /locus_tag="PWX02_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005727816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HDL1730285.1"
                     /translation="MSVLYAEHLAKSYKQRQVVKDVSLNVKSGEIVGLLGPNGAGKTT
                     TFYMVVGLVRHDHGTIRIDDQDISTLPMHDRAKQGIGYLPQEASIFRRLSVYDNLMSV
                     LQVRKNINDKQRKERAEELITEFHIEHIRNSLGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKKIIVNLKERGLGVLITDHNVRETLDVCERAYIVGSGEMIAS
                     GTPQQVLENPDVKRVYLGDQFKL"
     gene            complement(1995..>2229)
                     /locus_tag="PWX02_002322"
     CDS             complement(1995..>2229)
                     /locus_tag="PWX02_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HDL1730286.1"
                     /translation="LDNGKPVNGKGNSVHYDLNSEFLTLIGNAELKQQGSFIKANKIT
                     YDVKKQQLKATSGGKTRVKTVLIPNELQDNKKK"
BASE COUNT          598 a          448 c          457 g          726 t
ORIGIN      
        1 gcagccacat ttctaaataa ttgcgggttt ggtaaataaa attatgcact tcggtttggc
       61 gttctaagaa atcaatcacc ggttgctcta agcctgtcat tgggcgaagc tccggattcc
      121 aatgtgggtt cggtaaaaag cgaacatcaa aaacgtaatc ggaatcggca ggtaagccgt
      181 atttaaaacc gaatgattgg aaaataattt taagctcttt ttcggttgag ccttgcagta
      241 tttggcgaag attttccgct aattcgtgcg aagaaagatg actggtgtca ataatataat
      301 ttgcattttg caccaaaggc tcaagcaatt tttgttccaa atccaatgca ccttctaacg
      361 agagatcgtc ttgggtggaa agcgggtgga ttcggcggga atcgctataa cggcggatta
      421 aggtattgcg ttcgcaatct aaaaaaatca gcttagcatt taaaggctca agctgggtaa
      481 gcaattcttc aatcgcatta ggcgtattcg gtaaattacg aatatctaag ctgatgactg
      541 ccgaacgatt ttcttgagta aggtagtagg ttaaggttgg aattaaaggt aaagggatat
      601 tatccacgca gtaataacct gcatcttcta atgctcttaa tgctaccgat ttaccagagc
      661 ctgagcgtcc gcttataatg attaattcca tatttcctca gtattattct tcatctttta
      721 ggtgattatc agcatacacc aaaatttgcc aaatatcttc tgcggattca gctgctcgga
      781 gctgttttaa taacgattta tcggtaagtt tctgtgcaat tgcaggtagg caacttttat
      841 actgttcgca gctattttcc ggaaacatca ttgcataaat taaatccact tctttgttgt
      901 cttgggcttc gtagtcaatc gcttcttcca gctgtaaaaa gaccgcaatc ggtttagtca
      961 gctcaatatc accgcaagcg ggcaattttg catgaggaag tgcaatgcct tgatttaagc
     1021 cggttgtgcc taacttttca cgtttaaata aattgccaaa gcattcaata gggcagagtg
     1081 cttcagattg ctgttcggtt tggttgattg attcagccac gattttaccc acgagttcta
     1141 acgctcgttt tttacttgaa atcagtacac cttggcgaac attttcaggg cttagaaatt
     1201 cggttagttt tgtcataatt tttcatctaa agatgaaatc cccaaagagg ggatttttta
     1261 gagtttgaat tgatcgccta aataaacacg ttttacatca gggttttcaa gcacttgctg
     1321 tggtgtgccg ctggcaatca tttcgccgct accgacaatg taggctcttt cacatacatc
     1381 tagagtttca cgtacgttat ggtcggtaat caatacccct aaaccacgct cttttaaatt
     1441 gacgataatt tttttaatat caattaccga gattgggtca accccagcaa aaggttcatc
     1501 taacagaata aatttcgggt tcgctgctaa tgctcgggca atttccacac ggcggcgttc
     1561 accacctgaa agtgattgac ctaaactatt gcgaatatgc tcaatatgga attctgtaat
     1621 cagctcttcg gcacgctctt tacgctgttt atcattgata tttttacgca cttgtaatac
     1681 cgacattaag ttatcgtaca cacttaaacg gcggaaaata gatgcttctt gcggtagata
     1741 gccaatccct tgttttgctc tgtcgtgcat tggtagtgtg ctgatatctt ggtcgtcaat
     1801 acgaattgtg ccgtggtcgt ggcgaaccaa acccacaacc atatagaaag tggtggtttt
     1861 acctgcgccg ttcggaccga gcaagcccac gatttcacct gatttgacat tcaggctgac
     1921 gtcttttacc acttggcgtt gtttatagct ttttgctaaa tgttcggcat aaagcacaga
     1981 catatcattt aaccttattt tttcttgtta tcttgaagtt catttggaat tagcacggtt
     2041 ttcactcggg ttttaccgcc actggtagct ttaagttgtt gttttttcac atcataggta
     2101 attttattgg ctttaataaa gctaccttgt tgtttgagtt ccgcattgcc aatcagcgtt
     2161 aaaaattcag agtttaaatc ataatgaacg ctgttgcctt taccgtttac cggtttaccg
     2221 ttatctaag
//
LOCUS       DANJWC010000222         2073 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.223, whole genome shotgun sequence.
ACCESSION   DANJWC010000222 DANJWC010000000
VERSION     DANJWC010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.223"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            104..1249
                     /gene="lldD"
                     /locus_tag="PWX02_002323"
     CDS             104..1249
                     /gene="lldD"
                     /locus_tag="PWX02_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent L-lactate dehydrogenase LldD"
                     /protein_id="HDL1730287.1"
                     /translation="MIISSANDYRRAAKRRVPPFMFHYADGGSYAEQTLRRNVSDLED
                     IALRQRVLKDMSQLDTGIELFGEKLSMPVTLAPVGALGMYARRGEVQAGKAADNKGIP
                     FTLSTVSICPIEEVAPAIKRPMWFQLYVLKDRGFMKNALERAKAAGCSTLVFTVDMPT
                     PGARYRDMHSGMSGPYKDIRRVLQAMVHPFWAWDVGIHGKPHTLGNVSNYMGKPIDLN
                     NYIGWLTDNFDPSISWKDLEWIREFWDGPMVIKGILDPEDAKDAVRFGADGIIVSNHG
                     GRQLDGVLSSAKALPSIADAVKGEIKILADSGIRNGLDVVRMLALGADCTMIGRSFVY
                     ALSAAGQAGVENLLDIFLKEMKVAMTLTSNAKISDIGRDALVDLSNL"
     gene            complement(1303..2013)
                     /locus_tag="PWX02_002324"
     CDS             complement(1303..2013)
                     /locus_tag="PWX02_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730288.1"
                     /translation="MTNQKFINSSLTLAFFTLISFSTAYCYAWGIAAFHGYPWWHVEV
                     GNAGIARALAYVFGTFLTIFLFYLIGYALVNKVFKLHYFKYLGWLRVSVLVTIFSLPV
                     MISFYLFIGKVPMYLHLLYLGTTTLSVLLFHKHWDHRVFHLDIQKMLSEERFGFFYVF
                     IFIYFSLLSLSIGYIRPELRTTYDYLEIENKRYYVLSIHRNNIYVLGEKTKDNDEFLF
                     FNQDTLKYYRINITKMPN"
BASE COUNT          626 a          421 c          470 g          556 t
ORIGIN      
        1 aaaatgtgat ctatttctca tattttgcaa ataatagtaa ttcttatttt tattttaaag
       61 tagaatagcc acactttttt cttaatgatt tatgaggttt taaatgatca tttcatctgc
      121 aaacgattac cgccgtgcgg caaaacgtcg tgttccccct tttatgttcc attatgcgga
      181 tggcggttct tacgccgagc agaccttacg tcgtaatgtg agcgatttgg aagacatcgc
      241 cctgcgtcaa cgtgtgttaa aagatatgtc gcaactagac accggcatcg agctgttcgg
      301 cgaaaaactg tctatgccgg tgaccctcgc ccctgtcggt gcattaggaa tgtatgcccg
      361 ccgtggcgaa gtgcaagcgg gcaaagccgc agataacaaa ggtattccgt ttaccctttc
      421 cactgtctct atttgcccga ttgaagaggt tgcaccggca atcaaacgcc cgatgtggtt
      481 tcagctctat gtgttaaaag atcggggctt tatgaaaaat gcattggaac gagccaaagc
      541 cgccggttgt tccacgctag tgtttaccgt agatatgccg acccccggcg cacgttatcg
      601 tgatatgcac tccggaatga gcggcccata caaagacatt cgccgtgtgt tgcaggcaat
      661 ggttcacccg ttttgggcgt gggacgtggg cattcacggt aaaccccaca cgttaggcaa
      721 tgtgtcaaac tatatgggca aaccgattga tctgaacaac tacatcggct ggctgaccga
      781 taatttcgac ccatcaattt cgtggaaaga tttagagtgg attcgtgaat tttgggacgg
      841 cccgatggtg atcaaaggca ttctcgaccc agaagatgct aaagatgccg taagatttgg
      901 tgcggacggt atcatcgtct ccaaccacgg tggtcgtcag ttagatgggg tactttcttc
      961 tgccaaagcc ctgccaagca ttgccgatgc agtaaaaggg gagatcaaaa tccttgccga
     1021 ctctggtatc cgcaacggct tagatgtggt gagaatgctg gctctcggcg cagattgtac
     1081 gatgatcggg cgttcctttg tttacgctct tagcgccgcc ggtcaagccg gtgtggaaaa
     1141 cctgctcgac atcttcctta aagaaatgaa agtggcgatg acacttacca gcaatgctaa
     1201 aattagcgac atcggcagag atgctttggt agatttaagt aatctttagc tcaaatagcc
     1261 acaatccgca cgttttaggt gcggattgtt attttagata ggctaatttg gcattttggt
     1321 aatattaatg cggtaatatt tcaatgtatc ttggttgaaa aagagaaatt catcgttatc
     1381 tttagttttt tcgcccagca cataaatgtt attgcgatga atagataaga cataatatcg
     1441 cttattttca atttcaagat aatcatacgt tgttctcagc tcagggcgaa tgtagccaat
     1501 actcaaagaa agtaagctaa aatagatgaa aataaaaaca tagaaaaaac cgaagcgttc
     1561 ttcgcttaac attttttgaa tatctaagtg gaatacgcgg tggtcccaat gtttgtgaaa
     1621 taataatact gatagggtag ttgtccctag atagagtagg tgtaaataca ttggcacttt
     1681 gccaataaac agatagaaac tgatcattac tggcaagcta aaaatagtaa caagaacact
     1741 cacccgtagc cagccaaggt atttaaagta atgcagctta aaaactttat taactaaagc
     1801 gtatcctatc aaataaaaaa gaaagatagt taaaaatgtg ccaaaaacgt aggctagtgc
     1861 gcgagctata ccggcattac caacctcaac atgccaccac gggtagccat gaaaagcagc
     1921 aataccccaa gcataacagt aagcggtaga aaaagagatt aaagtaaaga aagcgagagt
     1981 aagagaagag ttgatgaatt tctggttagt cataacagta aatagattgc acctacttag
     2041 ttaaagatga aacacaagaa agcagactga gtt
//
LOCUS       DANJWC010000223         2063 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.224, whole genome shotgun sequence.
ACCESSION   DANJWC010000223 DANJWC010000000
VERSION     DANJWC010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.224"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(16..1731)
                     /gene="proS"
                     /locus_tag="PWX02_002325"
     CDS             complement(16..1731)
                     /gene="proS"
                     /locus_tag="PWX02_002325"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDL1730289.1"
                     /translation="MRTSQYLFSTLKEMPNDAQVVSHQLMLRAGMIRPMASGLYNWLP
                     TGIKVLKKVENIIREEMNKGGAIEVLMPVVQPAELWVESGRWNDYGPELLRFKDRGER
                     DFVLGPTHEEVITDLVRREVSSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFVMKDA
                     YSFHTTQESLQETYDVMYQVYSNIFTRLGLDFRAVQADTGSIGGSASHEFQVLASSGE
                     DDVVFSTESDFAANIELAEAVAVGERQAPTAEMQLVDTPNAKTINELVENHGLAIEKT
                     VKTLVVKGATEEQPLIALVIRGDHELNEIKAQKHPLVADPLEFADEAEIKAKIGAGVG
                     SLGVVNMPIPVIIDRSVALMSDFGCGANIDGKHYFNVNWERDVAVPEIADLRNVVEGD
                     PSPDGKGTLQIKRGIEVGHIFQLGKKYSEAMKATVQGEDGKPLVMTMGCYGIGVTRVV
                     AAAIEQHYDDRGIIWPCDEMAPFTVAIVPMNMHKSEKVQAFAEELYANLKAQGVEVIF
                     DDRKERPGVMFADMELIGVPHMVVIGEKNLENGEIEYKNRRSGEKQMIAKESLLAFLK
                     ANVKA"
     gene            complement(1895..2041)
                     /locus_tag="PWX02_002326"
     CDS             complement(1895..2041)
                     /locus_tag="PWX02_002326"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730290.1"
                     /translation="MFKEKDYDEYLEAKVKKGLAEIEKGQIFTLEQANAEWQEIIEQI
                     EREN"
BASE COUNT          534 a          524 c          368 g          637 t
ORIGIN      
        1 ttttgttatt tataattaag cctttacatt cgcttttaag aacgcaagta agctctcttt
       61 ggcgatcatc tgtttttcac cgctgcggcg gtttttgtat tcgatttcgc cgttctcgag
      121 gtttttctca ccaataacca ccatatgcgg cacaccgata agctccatat ccgcgaacat
      181 cacgcccggg cgttctttac gatcatcaaa aatcacctca acgccttgag cttttaagtt
      241 agcgtataac tcttcagcga atgcctgcac tttttccgat ttgtgcatat tcatcggcac
      301 aatcgccacc gtaaatggcg ccatttcatc acaaggccag ataataccac gatcatcata
      361 atgttgttca atcgcagctg ccaccacacg ggtaacgccg ataccgtaac aacccattgt
      421 catcactagc ggtttaccgt cttcaccttg cacagtggct ttcattgctt ctgagtattt
      481 tttgcctaat tggaaaatat gccctacttc aatacctcgt ttgatttgaa gtgtgccttt
      541 accgtccggg cttgggtcgc cttccaccac attgcgtaaa tctgcaattt ccggcacagc
      601 aacatcacgc tcccagttca cgttaaagta gtgtttgcca tcaatattcg caccgcagcc
      661 aaaatctgac atcaacgcca ctgagcggtc aataatcacc ggaatcggca tattcaccac
      721 acctaacgag ccaacaccgg caccgatttt ggctttaatt tccgcttcat cggcaaactc
      781 aagcggatca gccaccaacg ggtgtttttg agctttaatt tcgtttaact cgtggtcgcc
      841 acgaatcact aacgcaatta acggttgctc ttcagttgcc cctttcacca ctaaggtttt
      901 caccgttttt tcaatggcta aaccgtgatt ttctactaat tcattgatgg ttttcgcatt
      961 tggcgtatcc actaattgca tttctgccgt tggtgcttgg cgttcaccca ccgcaaccgc
     1021 ttcggctaat tcaatattgg cagcaaaatc tgattcggtt gaaaatacca catcatcttc
     1081 accgcttgag gctaacactt ggaactcgtg agaggctgaa ccgccgatag agccggtatc
     1141 tgcctgcacc gcacggaaat ctaagcccaa acgggtgaaa atattgctgt aaacctgata
     1201 catcacatca taggtttctt gcaggctctc ttgtgtggtg tggaaagaat aggcatcttt
     1261 catcacaaat tcacgcgaac gcattacccc gaaacgaggg cggacttcat cacggaattt
     1321 cgtttgaatt tggtataaat tcagcggaag ttgtttgtac gaagaaactt cacggcgaac
     1381 caagtcggta atcacttctt cgtgagtcgg gccaagcacg aaatcacgct cgccacgatc
     1441 tttaaaacgt aataattccg ggccgtaatc attccaacga ccggattcca cccataattc
     1501 cgccggctga accaccggca ttaatacctc gatagccccg cctttgttca tctcttcacg
     1561 aataatattt tccacttttt tcaacacttt gatcccggtc ggcaaccagt tgtataaacc
     1621 tgacgccatc gggcgaatca ttccggcacg caacattaat tgatggctaa ctacttgtgc
     1681 atcattcggc atttctttaa gggttgaaaa taaatattga cttgtacgca ttgctattcc
     1741 tattcttttt aaactttgaa acctgaaaaa atctgcttat tttaaaacaa agcggttaaa
     1801 ttttcaggaa attttgcaaa atcctataaa aaaataaccg cttgtaagcg gttattttgt
     1861 tttaactaag ggtattttct taaactgtgt atgcttaatt ttctcgttca atttgctcaa
     1921 taatctcttg ccattcggca tttgcttgtt caagagtaaa aatctgacct ttttcgattt
     1981 ctgccagacc tttttttact ttagcctcta aatattcatc ataatctttt tctttaaaca
     2041 ttattccccc gaatacattg ttt
//
LOCUS       DANJWC010000224         1987 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.225, whole genome shotgun sequence.
ACCESSION   DANJWC010000224 DANJWC010000000
VERSION     DANJWC010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.225"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..888)
                     /locus_tag="PWX02_002327"
     CDS             complement(<1..888)
                     /locus_tag="PWX02_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL1730291.1"
                     /translation="MLERIKSEWDKWVRLTPSNSLYDRTLIWLFLGLLIIGFVMVTSA
                     SLPVSTRLNNDPFYFAIRDGLYIIASIIFCYVFVQIPIEKWEKHNLALFFISIGFLIA
                     VLIFGRSINGAVRWIPLGILNFQPAELAKLAVICYFASFYVRKYDEIRKEKASFWRPA
                     VILFLFGFLLILQPDLGSTFVLFVLTFSMLFIVGAKIMQFMFLGVVGTVLFAVLILTS
                     EYRLKRVTSFMDPFADAYGDGFQLSNSQMAFGQGEFWGQGLGNSVQKLEYLPEAHTDF
                     VMAVIGEEFGFFGIACIVLL"
     gene            complement(888..>1987)
                     /gene="murD"
                     /locus_tag="PWX02_002328"
     CDS             complement(888..>1987)
                     /gene="murD"
                     /locus_tag="PWX02_002328"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDL1730292.1"
                     /translation="LIVISPGLAVATPEIQQAIQAGVEIVGDVELFCREAKAPIIAIT
                     GSNGKSTVTTLTTEMAKQAGIKVGMGGNIGVPVLSLLEGDYDLFVLELSSFQLETTYS
                     LKAKAATVLNISEDHMDRYDSIADYRQAKLRIYDNAENIIVNGEDAQTYPIQAVKKVI
                     RFAEQNAEYSLRNQMLYSGDDAIIQTKQMLISGRHNEMNALAAMALAEAANVPREGIV
                     KALQIYGGLPHRFQAVATNDGVRWVNDSKATNVGSTVAALNGLNIAGTLYLLLGGDGK
                     AADFSELKPLINKPNIVCYCFGQDGAALAELSEQSVLVETMQQAIEAIRPQLKKGDMV
                     LLSPACASLDQFKNFEQRGEVFMALAQGQHA"
BASE COUNT          612 a          474 c          363 g          538 t
ORIGIN      
        1 taaaagtacg atgcaagcaa taccaaagaa accaaattct tcaccaataa ccgccattac
       61 aaagtcagta tgtgcctctg gcaaatattc tagcttttgt accgaattac ctaaaccttg
      121 tccccaaaat tcaccttgtc caaatgccat ttgtgagttc gagagctgga aaccgtcgcc
      181 ataagcatca gcaaacggat ccataaagga ggtaacacgt tttaaacggt attccgaggt
      241 taaaatcaac accgcaaata acaccgttcc caccacgcct aaaaacataa attgcataat
      301 ttttgccccc acaatgaaaa gcattgagaa agtgagaaca aataatacaa aggtactacc
      361 taaatccggc tgcaaaatca gcaagaagcc gaataagaat aaaatcactg ccggtcgcca
      421 gaaactggct ttttctttac gaatttcatc atatttacgt acatagaaac tggcaaaata
      481 gcaaatgacc gctaatttgg ctaattctgc cggctgaaag ttgagaatac ccaacggaat
      541 ccaacgcact gcaccattaa ttgagcggcc gaaaatgagt acggcgatta aaaaaccgat
      601 tgaaataaaa aacagtgcca agttatgctt ttcccatttt tcaattggga tttgtacaaa
      661 aacataacaa aagataattg aagcaataat atacagcccg tctcgaatcg caaaataaaa
      721 tggatcgtta ttcaaccttg tgctaactgg gagtgaagca gatgttacca tcacaaaacc
      781 gataattagc aggcctaaaa atagccaaat tagagttctg tcataaagtg aattagaagg
      841 ggttaatctg acccatttat cccattccga cttaattctt tctaacatta tgcgtgctgt
      901 ccttgtgcta atgccataaa tacctcgcca cgttgctcaa agtttttaaa ctgatctaaa
      961 ctggcacaag ccggagaaag cagtaccata tcgccttttt tcagttgtgg gcggattgct
     1021 tcaattgctt gctgcatcgt ttcaaccaac acactttgtt cgcttaattc tgccaatgcc
     1081 gctccatctt gcccgaaaca atagcagaca atatttggct tattaattaa tggtttgagt
     1141 tcggaaaaat ccgcagcctt gccgtcacca cctaaaagta aataaagtgt accggcaata
     1201 ttcaagccgt ttaatgccgc taccgtgcta cccacattgg tcgctttgga atcattgacc
     1261 caacgcacgc catcattggt tgccaccgct tggaaacggt gtggcaaacc accataaatt
     1321 tgcaaagctt tcacaatgcc ttcacgagga acattcgccg cttctgctaa tgccatcgcc
     1381 gctaacgcat tcatctcatt atgacggccg ctaatcagca tctgtttagt ttggataatt
     1441 gcatcatcgc ctgaataaag catctgattg cgtaggctat attccgcatt ttgttctgca
     1501 aaacgaatca cttttttgac cgcttgtatc gggtaagttt gggcatcttc accgtttacg
     1561 atgatatttt ccgcattatc gtaaatccgc aatttcgctt ggcgatagtc ggcaatcgaa
     1621 tcgtagcgat ccatatgatc ttcgctaata tttaaaacag tggcagcttt tgctttcagt
     1681 gaataagttg tctcaagctg gaagctggat agctccagca caaaaaggtc ataatcgccc
     1741 tccagcaaac ttaataccgg cacaccgatg ttgccaccca ttcccacttt aatccccgct
     1801 tgcttcgcca tttcggtagt taaagtcgtt acggtacttt tgccgtttga accggtgatc
     1861 gcaataatcg gagctttcgc ttcccgacaa aacagttcaa catcaccgac tatttcaaca
     1921 ccagcttgaa tcgcttgctg aatttcaggt gtggcaacgg ctaaacctgg gctaattaca
     1981 ataagat
//
LOCUS       DANJWC010000225         1910 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.226, whole genome shotgun sequence.
ACCESSION   DANJWC010000225 DANJWC010000000
VERSION     DANJWC010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.226"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            182..1132
                     /gene="coaA"
                     /locus_tag="PWX02_002329"
     CDS             182..1132
                     /gene="coaA"
                     /locus_tag="PWX02_002329"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDL1730293.1"
                     /translation="MSLEKTSKITPFLTFDRQQWADLRKSVPLKLTEQDLKPLLGFNE
                     DLSLEEVRTIYLPLARLIQYYIEENLKRQTVLHRFLGIEPPKVPYIISIAGSVSVGKS
                     TSARILQALLSHYPKERKVDLITTDGFLYPLATLTEKNLLKKKGFPESYDIHSLIRFV
                     SDIKSGKRNVKAPIYSHLTYDIIPNQFNTVDQPDIVILEGLNVLQSGMNYPSSPHNVF
                     VSDFVDFSIYVDAEESLLKKWYINRFLKFRRSAFADPNSYFHHYASLSEQEAIETAST
                     IWEEINGLNLRQNILPSRERANLILIKGQDHAIETVKLRK"
     gene            1271..1810
                     /locus_tag="PWX02_002330"
     CDS             1271..1810
                     /locus_tag="PWX02_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HDL1730294.1"
                     /translation="MNKKFLLPLILFLALSVAFLVQLARNSQGDDPKKLESALIGKTV
                     PSFNLESVLDEKQTIDQSIVKTGKPRLLNVWATWCPTCYAEHEYLTILAKQGVEIVGI
                     DYKDERPKAIKFLANYGNPYKAIIFDPKGSLGLDLGVYGAPETFIIDGNGVIQYRHAG
                     DVNDQVWNQVLKPIYEKLK"
BASE COUNT          618 a          391 c          328 g          573 t
ORIGIN      
        1 aaaaaaatat taaaaaaagc gttctttcga tttttaaata tacaaaatga ctattttttg
       61 taaaatacaa cggattttct ctgcataact acggcaaaat aagtgcatat attgtatagt
      121 tacgccctta atttggtatt taattacctt aatattccag caaaaaattg agattttggc
      181 tatgagcctt gaaaaaacca gcaaaattac tccttttctc acttttgatc gccaacaatg
      241 ggctgattta cgcaaatccg ttccattgaa gctgaccgag caagacctaa aaccgctttt
      301 aggctttaat gaagatcttt cattagaaga agtacgcact atttatttac cgcttgcccg
      361 tttaattcaa tactatatag aagaaaactt aaaacgccag acagtcttac atcgctttct
      421 cggcattgaa ccgccaaaag tcccttacat tattagtatt gccggcagcg tatcggttgg
      481 gaaaagtacc tctgcccgta ttctgcaagc tctgctctcc cattacccta aagagcgaaa
      541 agtggattta attaccaccg acggtttttt atatccactt gcaacgctaa ccgaaaagaa
      601 cttactcaag aaaaaaggct ttccggaatc ttatgatatt cacagcctga ttcgctttgt
      661 atctgatatt aaatccggca aacgcaatgt gaaagccccg atttattcgc acctaaccta
      721 cgatattatc ccaaatcaat ttaatacggt ggatcaaccc gatattgtga ttttagaagg
      781 gctaaacgtg ttacaaagtg gaatgaacta cccatccagc ccacataatg tatttgtatc
      841 ggactttgtg gatttttcta tttatgtgga tgccgaagaa agtttgctga aaaaatggta
      901 tatcaaccgt ttcttgaaat tccgtcgcag tgcctttgcc gacccaaact cctatttcca
      961 ccattatgcg agtttgtccg agcaagaagc gattgaaacc gcctcaacga tttgggaaga
     1021 gatcaatggc ttaaatttac gccaaaatat tctcccaagc cgagagcgtg ctaaccttat
     1081 tttaatcaaa gggcaagacc acgccattga aacggtaaaa ttacgcaaat aatttaactt
     1141 tttcgaccgc ttgtaatacc ttaaaatccg ttgaatacca ctcaacggat tttttattta
     1201 aatcaatgct tattcaacta ttttttggta agctaacgac tttgaaagtg attgatatta
     1261 ggcatataca atgaacaaaa aatttctact tcctttaatt ctctttttag cgttatctgt
     1321 ggcatttttg gttcaactcg ctcgcaactc gcaaggcgac gatccgaaaa aattagaatc
     1381 agccttaatc ggcaaaacag ttccgagctt taatttagaa tcggtattag atgaaaaaca
     1441 gacaatcgac caaagtatcg tcaaaaccgg taaacctcgc ctgcttaacg tgtgggcaac
     1501 ttggtgtccg acttgttatg ccgaacacga atacttaacc attttggcaa aacaaggggt
     1561 ggaaattgtc ggtattgatt ataaagacga acgcccaaaa gccatcaaat tcttagccaa
     1621 ttacggcaac ccttataaag ccattatttt tgacccgaaa ggttcacttg gtttagattt
     1681 aggtgtttat ggtgcacccg aaacctttat tattgacggt aacggcgtga ttcaataccg
     1741 ccacgccggc gatgtaaacg accaagtctg gaaccaggtg cttaaaccga tttatgagaa
     1801 attaaaataa aaatgaaata ctttttgaaa cagaagaaag taaaatcctc ttttaccagg
     1861 gaatataata tattagcaag catttctttg attgtaactc tattaattat
//
LOCUS       DANJWC010000226         1903 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.227, whole genome shotgun sequence.
ACCESSION   DANJWC010000226 DANJWC010000000
VERSION     DANJWC010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.227"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(52..1743)
                     /gene="dld"
                     /locus_tag="PWX02_002331"
     CDS             complement(52..1743)
                     /gene="dld"
                     /locus_tag="PWX02_002331"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDL1730295.1"
                     /translation="MSNNSLISQLTEIVGQSYIITDPTKTEAYRSGYRFGTGSAIAVV
                     KPTTLLEYWNVLKACVAADVIVISQAANTGLTGGSTPSGNDYDRDIVIINTMRMNGIQ
                     LINNANQVVCLPGSTLNELELLLKTHGREPHSVIGSSCIGASVIGGICNNSGGALVQR
                     GPAYTEMALFAQLNEDGTLELKNHLGIDLGDTPEEILENLQAQRYQRKDIQQACGKGH
                     DHAYCNHVRQIDEDSPARFNADPARHYEAAGSAGKLAVFAVRLDTFPLEKDTAVFYIG
                     TNHTDTLNDIRRYMLANFEVLPISGEYIHRDAFDIAADYGKDTFWVIKKLGTHRLPQL
                     FSLKAKVDRMAKKLTFLPANLSDKILQAISKMLPQHLPESLRDYRNQYEHHLIIKMGG
                     KGVQEARDYLARYFADTQKGGYLECNPTESQAAMLHRFAVASAAIRYRAIHDKEVEDI
                     VALDIALRRNDKAWFEVLPPEIESQITHKLYYGHFMCHVFHQDYIVKKGVDCLALEHQ
                     MLELLDKRGAQYPAEHNVGHLYEAKPTLRKFYRQLDPTNSFNPGIGQTSKKKYWK"
BASE COUNT          531 a          424 c          396 g          552 t
ORIGIN      
        1 tggcatagtt tttgacggat cagcaactat tttgtctact atgccttttt gttacttcca
       61 gtattttttc ttcgaggttt gcccgatccc cggattaaag ctgttggttg gatcaagctg
      121 ccggtagaat ttgcgtaagg tcggttttgc ttcgtaaaga tgaccgacat tatgctccgc
      181 agggtattgt gccccacgtt tatccagtaa ttctaacatc tgatgttcca acgccaaaca
      241 atctacacct tttttcacaa tatagtcttg atggaaaaca tggcacataa aatgcccata
      301 ataaagtttg tgcgtgattt gactctctat ttccggcggt agcacttcaa accacgcttt
      361 atcatttcgg cgaagggcaa tatctaaagc aacaatatct tccacctctt tatcgtgaat
      421 ggctcgatag cgaatcgctg ccgaagcaac ggcaaaacga tgtaacattg ccgcttggct
      481 ttcagtcgga ttacactcca aataaccgcc tttttgtgta tcagcaaaat aacgggcaag
      541 atagtctctt gcttcttgaa cgccttttcc acccattttg ataattaagt gatgttcata
      601 ctggttacgg tagtctcgaa gactttccgg caaatgttgc ggtaacattt ttgagattgc
      661 ctgtaatatt ttatcactga gatttgccgg taaaaaggtg agtttcttcg ccattctgtc
      721 cacttttgct ttaagtgaaa agagttgtgg taagcggtgt gtaccgagtt ttttaatcac
      781 ccaaaaagta tctttaccgt aatcagcggc aatatcaaag gcatcacgat gaatatattc
      841 accggaaatc ggcaatacct caaagttggc taacatatag cgacgaatat cgtttaatgt
      901 gtccgtatga tttgtcccga tataaaaaac agccgtgtct ttttcaagcg gaaaggtatc
      961 taatcgcacc gcaaacaccg ctaactttcc tgcactaccg gcagcctcgt agtggcgggc
     1021 aggatcggca ttaaaacggg caggggagtc ttcatcaatt tggcggacgt gattacaata
     1081 ggcgtgatcg tgtccttttc cgcacgcttg ctgaatatcc ttacgctgat aacgttgggc
     1141 ttgcagattt tctaaaattt cttccggcgt atcacctaaa tcaataccta aatggttttt
     1201 taattccagc gtgccgtctt catttaactg ggcaaacagt gccatttcag tataagccgg
     1261 cccacgttgc actaatgcac cccctgaatt attacaaata ccaccgataa ccgaagcccc
     1321 gatacaggac gatccgatca ccgaatgcgg ttcacgtccg tgcgttttca gcaacaattc
     1381 cagctcattt aatgtcgagc ccggtaagca aacgacttga ttggcattgt taattaactg
     1441 aatgccgttc attcgcattg tgttaatgat cacaatatcc cgatcataat cgttgccgct
     1501 tggtgttgaa ccaccggtta atccggtatt ggccgcttgt gagataacaa tgacatcggc
     1561 ggcgacacag gcttttaata cgttccagta ttctaataac gttgtaggtt ttaccactgc
     1621 aatcgcacta cccgtgccaa agcgataccc gctacgatag gcttccgtct tagtcgggtc
     1681 agtgatgata taagactgcc cgacaatttc agtaagctga gaaatcagtg aattatttga
     1741 cattatgctt ccctcaaagg tagaaataga tagggcagta tagcaataaa caaaagagag
     1801 caaagcagaa tgttacaaaa ttgtgaacta ctgcacagaa taaatgaaga aatgaagtaa
     1861 aagaaagcga gctaaaagag cgtcgcccgc tttatttgta gaa
//
LOCUS       DANJWC010000227         1842 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.228, whole genome shotgun sequence.
ACCESSION   DANJWC010000227 DANJWC010000000
VERSION     DANJWC010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.228"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(328..1569)
                     /locus_tag="PWX02_002332"
     CDS             complement(328..1569)
                     /locus_tag="PWX02_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HDL1730296.1"
                     /translation="MMNKRIYVFADWETFSQPELVGVLSSDVVRGKENFRFSYDNEWL
                     SSPYVQQIDPNLYLYPGEQYSTDTQNFHIFLDSCPDRWGRLLMKRREAALASQENRKP
                     KLLIESDYLLGVHDMNRMGALRFKTELNGPFLDNNTRLAAPPLSSLRELEFAAIQLES
                     SDNVDDPDYLKWLHMLISPGSSLGGARPKASVIDELNQLWIAKFPSRYDDYDIALWEY
                     LAYRLAIQAKIEMATCRIERFNQEHHTFLTQRFDRHCGKRRHFTSALTQLGYYDGDYE
                     ASYLEIAQFLTESGSNTRIDLAQLWRRIVFNIAISNTDDHLRNHGFIYAQEGWILSPA
                     YDLNPVPLSQGLHLNITDSDNRLDYQLAFDVADFFQLSKQQAVQIYDEVLKEVNNWQN
                     VAKEIGIPRQEQEQMKFAFNV"
     gene            complement(1569..1772)
                     /locus_tag="PWX02_002333"
     CDS             complement(1569..1772)
                     /locus_tag="PWX02_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL1730297.1"
                     /translation="MFTQTLLAQRTGLSRVTVRKIMNGDPTVAIGYYLMVLGVLGLEQ
                     DFTDVGKDDELGRKLQDIELLRK"
BASE COUNT          540 a          377 c          322 g          603 t
ORIGIN      
        1 gatgtctgcc atctgctagg cattatatat gcctagatta gtttcctaca tttgcgtttt
       61 taaggctacg cttccttgcg gatatggttg agaaaaaagt caagtgttac taatggtttt
      121 ataagatgtt ggtttgttct ggtgtctgcc accgctaggt ctttataccc tagattagta
      181 tcaggtcttt ctggtgcctg ccaccgctag gcctttacat cctagattaa tatcagattg
      241 acaatgagcc aacaactcat tgcgttatta aggtaacgct acttttttag aacatagact
      301 acgatgattg gttccacaag tcaattacta tacattaaat gcaaatttca tctgctcttg
      361 ctcttggcgt ggaataccaa tttcttttgc gacattttgc caattattaa cttctttcag
      421 tacttcatca taaatctgta cggcttgttg cttagataat tgaaaaaagt cagcaacatc
      481 aaaggcgagc tgataatcca atcggttatc gctgtctgtt atgtttaaat gtaatccttg
      541 agatagtggt actgggttta aatcgtatgc gggagataat atccacccct cttgagcgta
      601 gataaatccg tggttacgca aatgatcatc agtattcgaa atagcaatat tgaatacaat
      661 tcttctccat agttgagcca agtcaattcg agtatttgaa ccactttctg ttaaaaattg
      721 agcaatctca aggtagcttg cctcatagtc tccgtcataa taacccagct gggttaatgc
      781 tgacgtaaaa tggcgacgtt ttccacaatg tcgatcaaat ctttgtgtca aaaaagtatg
      841 gtgttcttgg ttaaagcgtt caattctaca agtagccatt tcaattttgg cttgaatagc
      901 cagtcgataa gctaaatatt cccaaagagc aatatcataa tcatcgtacc gacttggaaa
      961 tttggcaatc catagttgat ttagttcatc tattaccgaa gcttttggtc tagctccgcc
     1021 tagtgacgac cctggtgata tcaacatatg aagccatttt aaataatccg gatcgtccac
     1081 attatcactg ctttcaagtt gtattgctgc aaattctaat tctcttaagg aagacagtgg
     1141 tggtgctgca agacgagtat tattgtccaa aaatggacca ttcaattcag ttttaaaacg
     1201 taaagccccc attctattca tatcgtgtac gcctaaaagg taatcacttt ctattagtaa
     1261 cttaggttta cgattctctt gactagctag agctgcctcc cgacgtttca ttaacaatct
     1321 accccatcta tcagggcaag aatctaaaaa gatatgaaaa ttttgtgtat ctgtactgta
     1381 ttgctctcca gggtaaaggt ataagttagg atcaatctgc tgtacatagg gtgaacttaa
     1441 ccactcgtta tcgtagctaa aacggaaatt ttctttgcct ctcactacat cagaagaaag
     1501 caccccaact aattcaggct gtgaaaaagt ttcccaatct gcaaacacat aaattcgttt
     1561 attcatcatt atttccttaa taattcaata tcttgcaatt tacgtcctaa ctcatcatcc
     1621 tttccaacat cagtgaaatc ttgttctaga ccgagaacac ctaaaaccat taggtagtat
     1681 ccaatcgcaa cagttggatc accattcata atttttctca ctgttacacg gctaagccca
     1741 gttctttgag ccaataatgt ttgagtaaac attctccttt ttgaggctag cctaatattt
     1801 tctccaagtt gaagcaaggc attttggtac ttaggaaaca ga
//
LOCUS       DANJWC010000228         1825 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.229, whole genome shotgun sequence.
ACCESSION   DANJWC010000228 DANJWC010000000
VERSION     DANJWC010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.229"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            99..485
                     /locus_tag="PWX02_002334"
     CDS             99..485
                     /locus_tag="PWX02_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDL1730298.1"
                     /translation="MTVIHTENAPAAIGPYVQAVDLGNMVLTSGQIPVNPETGEIPSD
                     IVQQTRQSLNNVKAIIEQAGLTVADIVKTTVFVKDLNDFAKVNAEYQAFFQENEHPNF
                     PARSCVEVARLPKDVGIEIEAIAVRR"
     gene            517..1254
                     /gene="pgeF"
                     /locus_tag="PWX02_002335"
     CDS             517..1254
                     /gene="pgeF"
                     /locus_tag="PWX02_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HDL1730299.1"
                     /translation="MDKIIPNWNVPKHIHALTTLRTGGVSKPPFDSFNLGDHVNDEPQ
                     DIAQNRALLVEKFQLPQAPLFLTQTHSTRVIELPYSGDNIKADAIYTNQPNQVCLVMT
                     ADCLPVLFASQDGSEIAAAHAGWRGLCDGILEATVEKFNCPPHEISAWLGPAIGPNAF
                     QVGSEVADQFCAFDPRAKEAFIEDSTTSGKFLGNLYQIATQRLNKLGITAISGGEYCT
                     YSQPELFFSYRRDKQTGRMATLIWRTE"
     gene            1348..1423
                     /locus_tag="PWX02_002336"
     tRNA            1348..1423
                     /locus_tag="PWX02_002336"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1381..1383,aa:Ile,seq:cat)
     gene            1626..>1825
                     /locus_tag="PWX02_002337"
     CDS             1626..>1825
                     /locus_tag="PWX02_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HDL1730300.1"
                     /translation="MTATKKTLSDTLLKNLKYSPNAKPIADSNGLFILAQKKAKVWIY
                     SYISPKTGKRSTKNRIGSYPQM"
BASE COUNT          572 a          402 c          355 g          496 t
ORIGIN      
        1 agaaacatta atgctcatta attcacttct ctttattcca aataataaga aaagatatta
       61 tcattaatcc gtttattttt cattaagagg aacacaagat gacagttatc cacacagaaa
      121 atgcaccggc agcgattggg ccttatgtgc aagcagttga tttaggcaat atggttttaa
      181 cttctgggca aattcccgtg aatcctgaaa ccggcgaaat cccgagtgat attgtgcaac
      241 aaacccgcca atctctgaac aacgtgaaag ccattatcga acaagccggc ttaaccgttg
      301 ccgatattgt aaagaccacc gtatttgtca aagatcttaa cgacttcgca aaggtaaatg
      361 cggaatacca agccttcttc caagaaaacg aacaccctaa ttttccggct cgttcttgcg
      421 tagaagtggc tcgtttacca aaagatgttg gcattgagat cgaagcgatt gcagtacgcc
      481 gataacaagc ggtcattttc ttgagatttt ttgcaaatgg ataaaattat tccaaattgg
      541 aatgtgccta aacatattca cgcactgacg accttaagaa caggcggagt aagcaaaccg
      601 ccttttgaca gtttcaactt aggtgatcac gttaatgacg agccacaaga tatcgctcaa
      661 aatcgtgctt tattggttga gaaatttcaa ttaccacaag ctccgctgtt tttaacccaa
      721 actcacagca cccgagtgat tgaacttccc tattcaggtg ataatattaa agccgatgcg
      781 atttacacca atcaaccaaa ccaagtctgc ttagtgatga ctgcagattg tttgccggta
      841 ttatttgcca gccaagacgg gtcggaaatt gcggcggcac acgccggctg gcgtgggtta
      901 tgcgatggca tattagaggc gacggtagag aaattcaact gcccgccaca cgaaatatca
      961 gcttggctag ggccggctat cggaccgaat gcttttcaag tcgggagtga agtggcagac
     1021 caattctgtg cttttgaccc aagagcgaaa gaagccttta ttgaagacag cactacaagc
     1081 ggtaaatttt taggaaatct ttaccaaatt gccacccaac gtctgaataa attaggcatt
     1141 accgccattt caggcggtga atattgcacc tacagccaac cagaactgtt tttttcctac
     1201 cgccgagaca aacaaaccgg gcgaatggca accttaattt ggcgaacaga ataaccctat
     1261 tttctctcgt tcaataatta accgagtaat cttctgcctc tagacgacta ctcggttttt
     1321 ctctataatt ccaaccactt ttcaaatccc cccatagctc agtggttaga gcaggcgact
     1381 cataatcgct tggtcgctgg ttcaagtccg gctgggggga ccatttaagc gttgcatatt
     1441 gtctttcatt gtctcaaatt tactataaaa tcaagaactt agattatttt atagtctctt
     1501 attgtcttct ttagttgcat tgtatcttag cgtttatgat ccctaaaatg atccctattt
     1561 tgaaaacggg aaaatttagg gatcataaac ttcgataaac ccttatatat caaggattag
     1621 aagaaatgac tgcaactaaa aaaacacttt cagacacatt actaaaaaat cttaaatatt
     1681 ccccaaatgc gaagcctatc gcagacagta acgggctatt tattcttgca cagaaaaaag
     1741 cgaaagtatg gatttatagc tatatcagcc ccaaaacagg caagcgaagc acaaaaaata
     1801 gaatcggtag ctatccgcaa atgag
//
LOCUS       DANJWC010000229         1782 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.230, whole genome shotgun sequence.
ACCESSION   DANJWC010000229 DANJWC010000000
VERSION     DANJWC010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.230"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            150..1181
                     /locus_tag="PWX02_002338"
     CDS             150..1181
                     /locus_tag="PWX02_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDL1730301.1"
                     /translation="MNIKATLRKARLAIGKAILDSKNTDLILSEIPNKILFLRHDGKI
                     GDYVVSSFVFREIKKQSPQTQIGVVCSQKNRYLFENNPYIDKLYLVKTKSIPDYIRGG
                     KQLAKEQYDVVIDPTVTLRNRDLLFLRTISAKHYVGYQKQKYRLFDLNVEDRKQHFSE
                     IYQDALALIGFENIETQYDVPLNATSNQSIKHYLYENQIRNYIALNLFGAGSARRFSE
                     QKINELLSCLKQHSDKPIVLLTFPEVTAKLCSFTKKYKNIFVYEKTKSVFDSIELIRF
                     ADLLISPDTATVHIASGLSKKIIAFYSSDEQNFIHWHPNNQAETHILRFRKSVNDLDF
                     AAIKPEWLK"
     gene            1183..>1782
                     /gene="ilvY"
                     /locus_tag="PWX02_002339"
     CDS             1183..>1782
                     /gene="ilvY"
                     /locus_tag="PWX02_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HDL1730302.1"
                     /translation="MDFQSLKLFLDIAQSRSFIRSAEKNYMTASTLTRHIQRIEEELG
                     QPLFLRDNRQVRLTEAGERFLAFAQQGWAEWQQLQNQLSPAQGDLEGELKVFCSVTAS
                     YSHLPPILGKFRQKYPKVDIKLSTGDPALALEQVQSLAADISVAGKPEFLPNNIVFHY
                     IDDIQLSIIAPRVASQATQFLQQSPIDWKNIPFILPVNGP"
BASE COUNT          559 a          301 c          359 g          563 t
ORIGIN      
        1 agcatctccg ataggtttga gtaatacgga ttctattttg ctaaatttga aggttttttt
       61 ctcttttttt gtaccaaaaa gttttataac taaggatttc atctctataa taggttgatt
      121 cttttaatga ttggttatag taagtaaaaa tgaatattaa agcaacgtta agaaaagcaa
      181 gattggcgat aggtaaagcc attttagata gtaaaaatac tgatctgata ttatcggaaa
      241 ttcccaataa aattctattt cttcgtcacg atggtaaaat tggtgattat gtcgttagtt
      301 cctttgtctt ccgtgaaata aaaaaacagt ctccccaaac gcaaataggc gtggtttgct
      361 cacagaaaaa ccgatattta tttgagaata atccttatat tgataagctc tatttggtta
      421 agacaaaaag tattcctgat tatattcgtg gtggcaaaca acttgcaaaa gagcagtatg
      481 atgttgtaat cgatccgact gttactttac ggaatcgtga tctcttattt ttacgcacta
      541 tatcagccaa acattatgta ggttatcaga aacaaaaata tcgattattt gatctaaatg
      601 tagaggatag aaaacagcat ttttcagaga tttatcaaga tgcgttagct ttaatcgggt
      661 ttgaaaatat agaaactcag tatgatgtgc cactaaatgc cacaagcaac caatccataa
      721 agcattattt atatgaaaac caaattagaa attatattgc gttaaatctt tttggtgcag
      781 gttcggcaag acgttttagt gagcagaaaa ttaatgaact gctgagttgc ttaaaacagc
      841 atagtgataa accgatagtc ttacttactt ttcctgaagt aacggcaaaa ttgtgttcct
      901 ttactaagaa gtataaaaat atatttgtct atgagaaaac caaaagcgtt tttgattcaa
      961 tagaactgat tcgttttgcg gacttattaa tttcacctga tactgcaacc gtgcatattg
     1021 cctcaggctt gagtaaaaag ataattgctt tttacagtag cgatgaacag aattttatac
     1081 attggcatcc gaataatcag gctgaaaccc atattctccg ttttagaaaa agcgtgaatg
     1141 atttagattt tgctgcaata aaaccggagt ggctaaaata atatggactt ccaaagcctt
     1201 aaacttttcc tagatatagc ccaaagccgt agttttattc gcagtgcgga gaagaattat
     1261 atgacggctt ccacacttac ccgccatatt cagcgtatag aggaggagct cgggcagccg
     1321 ttgtttctgc gtgataatcg acaagtgcgt ttaactgaag caggggaaag gtttttggcg
     1381 tttgctcaac aaggttgggc agagtggcag cagttgcaaa accagttgtc gcctgctcaa
     1441 ggtgatttag aaggagagtt gaaagtgttc tgctcggtaa cggcttctta cagccattta
     1501 ccgccgattt tgggaaagtt tcgccaaaaa tatccgaaag tggatattaa actgagtacc
     1561 ggtgatccgg ctttggcatt ggaacaggtt cagtcattgg ctgcagatat ttcggttgcg
     1621 ggtaagccgg aatttctgcc gaataatatt gtgtttcact atattgatga tattcaactt
     1681 tcgattattg cccctcgtgt ggcgagccaa gccactcaat ttctgcaaca gtcgccgata
     1741 gattggaaaa atatcccctt tattttgccg gtgaacggac ct
//
LOCUS       DANJWC010000230         1739 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.231, whole genome shotgun sequence.
ACCESSION   DANJWC010000230 DANJWC010000000
VERSION     DANJWC010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.231"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(34..159)
                     /gene="ykgO"
                     /locus_tag="PWX02_002340"
     CDS             complement(34..159)
                     /gene="ykgO"
                     /locus_tag="PWX02_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HDL1730303.1"
                     /translation="MKILNSLKTAKSRHPDCQIVRRKGKLYVICKTNPRFKARQR"
     gene            complement(168..440)
                     /locus_tag="PWX02_002341"
     CDS             complement(168..440)
                     /locus_tag="PWX02_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDL1730304.1"
                     /translation="MKKGIHPENYREVLFYDASVQQGWVIRSCAATNKTMVWEDGKEY
                     PLYTLDTSSASHPVYTGKRREVNTEGRASQFKDRFKGFASTLSNQK"
     gene            634..1311
                     /gene="rpe"
                     /locus_tag="PWX02_002342"
     CDS             634..1311
                     /gene="rpe"
                     /locus_tag="PWX02_002342"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDL1730305.1"
                     /translation="MSKNPYLIAPSILSADLARLGDDVAEVLKAGADLIHFDVMDNHY
                     VPNLTFGPAICKALRDYGIQCPIDVHLMVKPVERLIPDFAKAGADYITFHPEATEHID
                     RNLQLIRDLGCKSGLVFNPATPLSYLDYVMDKVDVVLLMSVNPGFGGQSFIPSTLDKL
                     KEVRRRIDESGYNIRLEVDGGMKINNIADIAAAGADMFVAGSAIFDQPDYQAVIAQMR
                     DELRKVG"
     gene            1498..1728
                     /gene="acpP"
                     /locus_tag="PWX02_002343"
     CDS             1498..1728
                     /gene="acpP"
                     /locus_tag="PWX02_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL1730306.1"
                     /translation="MSIEERVKKIIVDQLGAKAEDVKPEASFIEDLGADSLDTVELVM
                     ALEEEFDIEIPDEEAEKITTVQSAIDYVQNNQ"
BASE COUNT          530 a          284 c          362 g          563 t
ORIGIN      
        1 ccttaataaa ttagcttatt aaggtgctgg tttttaacgc tgacgagctt taaatctcgg
       61 gttggttttg caaatcacat aaagtttgcc tttgcgtctg acaatttggc aatccgggtg
      121 gcgtgatttt gcggttttaa gtgagtttaa gattttcatt ttatcctcta tttttgattt
      181 gaaagcgttg aagcaaagcc tttaaatcta tctttaaatt gacttgcacg accttctgta
      241 ttcacttcac ggcgtttacc tgtgtaaaca gggtgagacg ccgatgaggt gtctaaggtg
      301 taaagtgggt attctttgcc atcttcccat accattgttt tgttggttgc cgcacaagaa
      361 cggataaccc agccttgttg cactgaggca tcatagaaca acacttcacg ataattttca
      421 ggatgaatcc cttttttcat attttctcca gaatttagat aaggtcatta accataatta
      481 aaatatggga tacataatat acgaacttta aatacaattc cataactttt ttatggtgtt
      541 ttgtaaaaaa acggattttt aggaagagag actcttttcg ctgttaaaat tatggtaatc
      601 taacgtagat ttattttttg atagtgagga aatatgagta agaatcctta tttaattgca
      661 ccgtctattt tatctgccga tttagctcgc ttgggtgatg atgtggcaga ggtgttaaaa
      721 gccggtgcag acttaattca ctttgatgta atggacaacc actacgtgcc gaatttgacc
      781 ttcgggccgg cgatctgcaa agccttacgt gattacggca ttcaatgccc gattgatgtg
      841 catttaatgg taaaaccggt tgagcgtttg attccggatt ttgccaaagc cggagcggat
      901 tatattacat tccaccctga agcaacggaa catatagatc gaaatttaca gcttatccgt
      961 gatttaggtt gtaaatcagg cttggtgttt aacccggcaa cgccattgag ctatttagat
     1021 tatgtgatgg ataaagttga cgtggtttta ttgatgtctg tgaatccggg ctttggcgga
     1081 caatcattta ttccgtccac cttagataaa ttgaaagaag tccgtcgtcg tattgatgag
     1141 agcggttata acattcgctt agaggttgat ggtggtatga aaataaataa cattgccgac
     1201 attgcagccg caggagcaga tatgtttgtg gcaggttctg cgatttttga ccagccagat
     1261 tatcaagcag tgattgcaca aatgcgagat gaattacgga aagtagggta atcaaatgaa
     1321 atttgattta tctctataga ttaaacacaa acataatgat agaatggaaa aacagatata
     1381 ttctggtgta taggaatagt ctggtttgac ctttttataa aaggttgtat ttaaacttat
     1441 ttcgattaca ctatttaact ctcgcactta tgcgatattc actataggaa aaacaaaatg
     1501 agcattgaag aacgcgtaaa aaaaatcatt gttgatcaac ttggcgcgaa agcagaagat
     1561 gtaaaaccag aagcttcatt cattgaagat ttaggtgcag attctttaga cactgttgaa
     1621 ttagtaatgg ctttagaaga agaattcgat atcgaaattc cagatgaaga agctgaaaaa
     1681 attactacag ttcaatcagc aattgattac gttcaaaaca atcaataatc tgaattaat
//
LOCUS       DANJWC010000231         1736 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.232, whole genome shotgun sequence.
ACCESSION   DANJWC010000231 DANJWC010000000
VERSION     DANJWC010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.232"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            49..1716
                     /gene="rpsA"
                     /locus_tag="PWX02_002344"
     CDS             49..1716
                     /gene="rpsA"
                     /locus_tag="PWX02_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDL1730307.1"
                     /translation="MSELSFAQLLEESFAATPRLGDVVKGTVVAIQKGLVIVDTGFKS
                     ESAIDASEFTNAQGELEVQVGDEVDVVLKAVEDGFGATVVSRGDAKRNEAWIALEKAF
                     EEQATVIGFVNGKVKGGFTVELNGVRAFLPGSLVDTRPVRDSLNLEGKEYEFKVIKLD
                     QKRNNVVVSRRAVIETESNQDRDAVLANLVEGSEVKGTVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEVVNVGDEVTVKVLKFDKDKTRVSLGLKQLGQDPWAVIAENHPV
                     NSKLTGKVTNLTDYGCFVEILDGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLE
                     VDEERRRISLGLKQCKANPWEQFAATHSKNDKVSGKIKSITDFGIFIGLEGGIDGLVH
                     LSDISWNVSGEEAVRNYKKGDEVEAVVLQVDAVKERISLGIKQLESDPFTNFVDGTKK
                     GAILTGKVVEVDAKGVKVELEGGVEAFIRANEATAERVEDITSVISVGDSIEAKYTGV
                     DRKSRVVNLSVRAKNEAEESAAIAQVNKEEVVVPNAFAEAFKAAKGE"
BASE COUNT          568 a          260 c          390 g          518 t
ORIGIN      
        1 ccgtttaaca ggacgttaga tggatgttta tttttaagaa gatttattat gtcagaatta
       61 tcgtttgctc aactacttga agaatcattt gcagctacac cacgtttagg tgatgttgtt
      121 aaaggtactg ttgttgctat ccaaaaaggc ttagttattg ttgatacagg tttcaaatct
      181 gaatctgcaa tcgatgcctc agaatttact aacgcacaag gtgaattaga agttcaagtg
      241 ggtgatgaag tagatgttgt tcttaaagct gtagaagacg gttttggtgc aacagtagtt
      301 tctcgtggtg atgcaaaacg taacgaagct tggattgctt tagagaaagc atttgaagaa
      361 caagctacag ttatcggttt cgtaaatggt aaagtaaaag gcggtttcac tgttgaatta
      421 aacggtgttc gtgcattctt acctggttca ttagttgata ctcgtccggt tcgtgattct
      481 ttaaacctag aaggtaaaga gtacgaattc aaagtaatca aattagatca aaaacgtaac
      541 aacgtagtag tatctcgtcg tgctgttatc gaaacagaaa gcaaccaaga tcgtgatgcg
      601 gtattagcaa acttagttga aggttctgaa gttaaaggta cagttaaaaa cttaactgac
      661 tacggtgcat tcgtagattt aggtggcgtt gacggtttat tacatatcac agatatggct
      721 tggaaacgtg ttaaacaccc aagtgaagtg gttaatgtag gcgatgaagt tactgttaaa
      781 gtattaaaat ttgacaaaga caaaactcgt gtatcattag gcttaaaaca attaggtcaa
      841 gatccttggg ctgtaattgc tgaaaaccac ccggtaaaca gcaaattaac tggtaaagta
      901 actaacttaa ctgactacgg ttgcttcgtt gagattttag acggtgttga aggtttagtt
      961 cacgtttcag aaatggattg gacaaacaaa aacattcacc catctaaagt ggttaacgta
     1021 ggcgatgtag ttgaagtaat ggtacttgaa gttgatgaag aacgtcgtcg tatctcatta
     1081 ggtttaaaac aatgtaaagc aaacccttgg gaacaatttg cggcaactca cagcaaaaat
     1141 gataaagtat caggtaaaat caaatcaatt actgacttcg gtattttcat cggtttagaa
     1201 ggtggtattg acggtttagt tcacttatct gacatttctt ggaatgtttc aggtgaagaa
     1261 gcagttcgta actacaaaaa aggtgatgaa gtagaagctg ttgttctaca agttgatgca
     1321 gtgaaagagc gtatctcttt aggtatcaaa caattagaat ctgatccttt cacaaacttt
     1381 gtagatggta ctaaaaaagg tgcaatctta acaggtaaag ttgtagaagt tgatgctaaa
     1441 ggcgttaaag ttgaattgga aggtggcgtt gaagcattta ttcgtgcaaa cgaagcaact
     1501 gctgagcgtg tagaagacat cacttctgtt atttctgttg gcgattcaat cgaagcgaaa
     1561 tacaccggtg ttgatcgtaa atctcgcgta gttaacttat ctgttcgtgc aaaaaacgaa
     1621 gctgaagaat cagcagcaat tgcacaagtg aacaaagagg aagttgttgt acctaatgca
     1681 tttgctgaag ctttcaaagc agctaaaggc gaataattag tatagactaa tctaaa
//
LOCUS       DANJWC010000232         1719 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.233, whole genome shotgun sequence.
ACCESSION   DANJWC010000232 DANJWC010000000
VERSION     DANJWC010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.233"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..389
                     /locus_tag="PWX02_002345"
     CDS             <1..389
                     /locus_tag="PWX02_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1730308.1"
                     /translation="GVLDNAMYLNDAGKMVEKQYKELENYFNNVTCLEYIIMPNHIHF
                     IIQILNVNVIQQSLFDIIQRFKSITTVEYIKNVRHNNWKPFHKKLWQRSYYEHIIRNE
                     EDYLSIVKYIENNPTNWQKDKLFIND"
     gene            complement(486..1715)
                     /locus_tag="PWX02_002346"
     CDS             complement(486..1715)
                     /locus_tag="PWX02_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HDL1730309.1"
                     /translation="MILQSCGECGQQVRVPLMLNGNAVCPVCHNILRKNNTWSLRRSA
                     FLALAILILLPFALHFPLMSIDLLGIKINATVWDGIWKMATEGYPYTAFMVLVCAVIM
                     PIAFVLLILTVQLQRIIRQRPRYTLIFLSKIKEWVMLDVYLVALAVAAFKIRDYADLQ
                     FDVNLIAFVLVTILNTLLFIKTDPKQAWERFYPEYHALPFDHPSNPTLCPTCEYCFDE
                     TILDLKGQQRCPRCESNLSISDNVKIQRVWACLIAGTIMMIPANIFPISTTELAGQVS
                     ADSLMSGVLLFMEMGSYTVAAIVFIASIAVPVSKITIIFYLLLAIHYKWKHPIHWQMK
                     LLHYVHFVGRWSMLDLFVLSLMMSLLERGQILSFSVGEAAFYFGAAVFLTMFASANLD
                     ARMLWKIHYDHKRKATH"
BASE COUNT          569 a          315 c          350 g          485 t
ORIGIN      
        1 gcggtgttct tgataatgcg atgtatttga atgatgcagg taaaatggta gaaaaacagt
       61 ataaagaatt agagaattat tttaataatg tcacttgttt agaatatata attatgccaa
      121 accatattca tttcataatc caaattctta atgtgaatgt tattcagcaa tctctatttg
      181 atattattca acgttttaaa tctataacga cagtggaata cattaaaaat gttagacaca
      241 ataattggaa accatttcat aagaagttat ggcaacgcag ttattacgaa catattatta
      301 gaaacgaaga agactattta agcatagtga aatatataga aaataatccg actaattggc
      361 aaaaagataa attatttatt aatgattagg tatagcaata tatgatatct attatgccta
      421 ttcctacttc cagacgggca gaagcccgtc ctaagctatt ttatgttttt aacgataaag
      481 ggaatttagt gggtagcttt gcgtttatga tcgtaatgaa tcttccacag cattctggca
      541 tctaaatttg cagaggcaaa catcgttaag aatacggctg caccaaaata gaaagcggct
      601 tcccctaccg agaaactgag aatctgccct cgttcgagta gcgacatcat caatgataac
      661 acgaataaat ccagcattga ccagcgtccg acaaaatgga cgtagtgcag tagtttcatt
      721 tgccaatgaa tcgggtgctt ccatttgtaa tgaattgcca gcaagagata aaaaataatg
      781 gtaattttac tcaccggcac ggcaatactg gcgataaaca caatcgctgc cacggtgtag
      841 ctacccattt ccataaacag aagcacgccg gacattaatg aatctgccga aacctgccct
      901 gccaattcag tggtcgaaat cgggaaaata tttgccggaa tcatcataat cgttcctgca
      961 attaagcaag cccaaacccg ttgtatttta acgttatcgg aaattgataa attgctttca
     1021 caacgagggc agcgttgttg tccttttaaa tccaagatgg tttcatcaaa gcaatattca
     1081 caggtcgggc aaagtgttgg attgcttggg tgatcaaacg gtaaggcgtg atattctggg
     1141 taaaaacgtt cccacgcttg tttgggatcg gttttaataa aaagtagggt gtttaagatg
     1201 gttaccaaca caaaggcgat taaatttacg tcaaattgta gatcggcata atctcgaatt
     1261 ttaaaagctg caactgcaag ggcaactaaa tagacatcaa gcattaccca ttctttgatt
     1321 ttgcttaaga aaatcagggt ataacggggg cgttggcgaa taatacgttg tagctgaacg
     1381 gttaaaatta gcaatacaaa tgcaatcggc attattaccg cacagactag caccataaaa
     1441 gcggtgtaag ggtagccttc ggttgccatc ttccaaatgc cgtcccatac ggtagcgtta
     1501 attttgatgc cgagtaagtc aatgctcatt agagggaaat gaagtgcaaa ggggagcaaa
     1561 atcaaaattg ctaacgccaa aaaagcactg cggcgtaagc tccaagtgtt gtttttgcgt
     1621 aatatgttat ggcataccgg gcaaacggca tttccattga gcataagcgg cactcgtacc
     1681 tgttgcccac attcgccaca gctttgtaga atgattttg
//
LOCUS       DANJWC010000233         1662 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.234, whole genome shotgun sequence.
ACCESSION   DANJWC010000233 DANJWC010000000
VERSION     DANJWC010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.234"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..>1662)
                     /locus_tag="PWX02_002347"
     CDS             complement(<1..>1662)
                     /locus_tag="PWX02_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730310.1"
                     /translation="VWEALSVLLPERFDFEVFEVIDYSKEWSWFEIIVENLFWRQPEK
                     INMQKLHQFFKNSPYFNQATWINILYQLATEINHPLNIKYLHNKLYSFSLADRDEIWT
                     AFIALESDQCPAIERLLTWCQKSSGQENIEPEALLLGAIAVSWLFTSTDKRSRNGYIN
                     ALAHLLWNRLEIALALFKEFIEVNDPYVMEGILSAIHGAVAYSENSSGLKALALEIDN
                     QIFNQPETEEIYPNVLVRNYARHIIEYSLQKDEYSSDDIEIIRERITPPYRSSFPQNL
                     PTNEEIDKKYHSDGQRIIIRSMTTEYGRGMCGYGDFGRYTFEAKFRNWESYKFGNLIN
                     VDLLSNYACQMIFEQFGYDDKKHLEFDKETRSRYWRRDDTNKIERIGKKYQWLALYEI
                     LARVMDNFPAFSNRYADEIGLEYITNLDNFYLPKTQIKIVPPTKTDVFFQYINQKQCK
                     TKHYFTIQNDELIDDWVKINDFPDVSNLLEVTINDEQWLVLERHDDFRELAKFGQKET
                     YDRQMWLQIRSYLVKEFQFSKAKNWLAQQDFMERWMPEGNEHYGMN"
BASE COUNT          541 a          305 c          225 g          591 t
ORIGIN      
        1 attcattcca tagtgttcat taccttctgg catccatctt tccataaaat cttgttgtgc
       61 caaccaattt tttgcttttg aaaattgaaa ttctttaact aaataacttc taatttgtaa
      121 ccacatttgc ctatcataag tttctttttg cccaaatttt gctaattcac gaaaatcatc
      181 gtgtctttct agaacaagcc actgttcatc attaatcgtt acttccaaaa gattactaac
      241 atcaggaaaa tcatttattt ttacccaatc atcaattaat tcatcatttt gaatggtaaa
      301 atagtgtttt gttttacatt gtttttgatt tatgtattga aaaaatacat ctgttttagt
      361 tggcggtaca attttaattt gggttttggg tagataaaag ttgtccaaat tagtaatata
      421 ttcaagccct atttcatcgg catagcgatt agaaaaagca ggaaaattat ccatcaccct
      481 tgccaagatt tcatacaaag ccaaccattg atatttcttg ccaattcgct caattttatt
      541 agtatcatct cttcgccaat atcttgaacg agtttcttta tcaaactcaa ggtgcttctt
      601 gtcatcatag ccaaattgct caaaaatcat ttggcaagca taattactta ataaatcaac
      661 attgatgaga ttgccgaatt tataactctc ccaatttcgg aatttggctt caaaagtata
      721 tctgccaaaa tcaccatatc cacacatacc acgaccatat tcagttgtca ttgaacggat
      781 aatgattctt tgtccatcag aatgatattt tttatcaatt tcttcattgg ttggtaagtt
      841 ttggggaaat gaacttctat aaggtggcgt aattctttct ctgattatct caatatcatc
      901 tgatgaatat tcatcttttt gcaaagagta ttcaataata tggcgagcat aattacgaac
      961 caaaacattg ggataaattt cttctgtttc aggttgatta aatatttgat tatctatctc
     1021 taatgcaagt gcttttaagc cagatgaatt ttcagaataa gcaacagctc cgtgaatggc
     1081 agataaaata ccttccataa cataagggtc attaacctct ataaattcct tgaataacgc
     1141 caatgcaatt tccaatctat tccataataa atgagccagt gcattaatat agccatttct
     1201 acttcgttta tcggtagaag taaatagcca actaacagca attgcaccca ataatagtgc
     1261 ttcaggttca atattttctt gaccacttga tttctgacac caagttagta atcgttcaat
     1321 tgctgggcat tgatcagact ccaaagcaat aaaagctgtc caaatctcat ctctatctgc
     1381 caaagaaaaa gaataaagtt tgttatgtaa atattttata tttaatgggt ggttaatttc
     1441 ggtagccagt tgatataaaa tattaatcca agttgcttga ttaaaatatg gactattttt
     1501 gaaaaattga tgtagttttt gcatattgat tttttcaggc tgcctccaaa agagattttc
     1561 tacaataatc tcaaaccaag accattcctt tgaataatca atcacttcaa atacttcaaa
     1621 atcaaatcgt tctggcaaca atacacttaa tgcttcccat ac
//
LOCUS       DANJWC010000234         1646 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.235, whole genome shotgun sequence.
ACCESSION   DANJWC010000234 DANJWC010000000
VERSION     DANJWC010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.235"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            5..451
                     /gene="matP"
                     /locus_tag="PWX02_002348"
     CDS             5..451
                     /gene="matP"
                     /locus_tag="PWX02_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HDL1730311.1"
                     /translation="MRYQKLITQETTWKWQYLLKKHREGENITKYEEQSLIDLKVQFL
                     STLQNSPDEVEKWIKSEMTPEQRKKMRQSVRAKRKRFFNAEKQTTKKKSIDLEYSSWL
                     RLSKYAKSQKLTLSEAIVKLTDELENKQLYLEQVAKMKSSLKDLLK"
     gene            complement(517..1635)
                     /gene="ompA"
                     /locus_tag="PWX02_002349"
     CDS             complement(517..1635)
                     /gene="ompA"
                     /locus_tag="PWX02_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_119863613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HDL1730312.1"
                     /translation="MKKTLVALAVLSAAAVAQAAPQANTFYAGAKAGWASFHDGLTQF
                     DHEGDGGGFDLKYGINRNSVTYGVFGGYQILNQNNFGLATELGYDYFGRVRGNTTFAD
                     DKERTAAKHSAHGAHLSLKPSYEVVPNLDVYGKVGVALVRNDYYVQQRVDKDSRIKAH
                     NLKPSLLLGAGLEYAITPELAARVEYQYLNRVGNVDKAVRKTVSIPTGTNFQYSPDIH
                     SVSAGLSYRFGQGAAPVEAPEVVTKNFAFSSDVLFDFGKSSLKPAAATSLDAAHAEIS
                     NLGLANLAIQVNGYTDRIGKEASNLKLSQRRAETVANYIVSKGTNPANVTAVGYGEAN
                     PVTGHTCDAVKGRKALIACLAPDRRVEIQVQGSKEVTM"
BASE COUNT          524 a          319 c          314 g          489 t
ORIGIN      
        1 gattatgcga tatcagaaac tcatcaccca agaaacaact tggaaatggc aatatctcct
       61 aaaaaaacat cgtgaaggag aaaatattac taagtacgaa gaacaaagct tgatcgattt
      121 aaaagttcag ttcctctcta ctttgcaaaa ctctccggac gaagtggaaa aatggattaa
      181 gtcggaaatg acgcctgagc aacgtaagaa aatgcgtcaa tcagtacgcg ccaaacgcaa
      241 acgcttcttt aatgctgaaa agcaaacaac caagaaaaaa tccattgatt tagagtattc
      301 gagctggtta aggctctcta aatatgccaa aagccaaaaa ctaacgcttt ctgaagctat
      361 tgtcaaatta accgatgaac tcgaaaataa acaactttac ttagagcaag ttgctaaaat
      421 gaaatcaagt ttaaaagatt tactgaaata aaaaaagaaa aaagcccact aacaagcggg
      481 ctttttttga ttcaattttt caactgaata actatattac atagttactt cttttgaacc
      541 ttgaacttgg atttcaacac gacgatctgg tgctaagcaa gcgattaatg ctttacgacc
      601 tttaactgca tcacatgtgt gaccagttac tgggtttgct tcaccgtaac ctacagctgt
      661 tacattagct gggttagtac ctttagaaac gatgtaatta gctactgttt cagcacgacg
      721 ttgtgaaagt tttaagttag atgcttcttt accgatacgg tctgtgtaac cattaacttg
      781 gatagctagg tttgctaagc ctaagttgct gatttcagcg tgtgctgcat ctaaagaagt
      841 tgctgctgct ggttttaagc ttgatttacc gaaatcaaat aaaacatcag agctgaatgc
      901 gaagttttta gttacaactt ctggtgcttc aactggtgct gcaccttgac cgaaacggta
      961 tgataaacct gcagatactg agtggatatc tgggctgtat tggaaatttg tacctgttgg
     1021 aatactaact gttttacgaa ctgccttatc tacgttacca acacgattta agtattgata
     1081 ttcaacacga gcagctaatt caggagtaat tgcatactca agaccagcac ctaataatag
     1141 agatggtttt aagttgtgag ctttaatgcg tgaatcttta tctacacgtt gttgtacgta
     1201 gtaatcgttg cgaactaatg caacacctac tttaccgtaa acatctaagt taggaacaac
     1261 ttcgtagctt ggttttaagc ttaagtgcgc accgtgagct gaatgtttag ccgcagttct
     1321 ttctttatca tcagcaaatg tagtgttacc acgaacacga ccaaagtagt cataacctaa
     1381 ttctgtcgct aaaccaaagt tgttttggtt taagatttgg taaccaccga atacaccgta
     1441 agttacagag ttacggttaa taccgtattt aagatcgaat ccaccaccat ctccttcgtg
     1501 gtcaaattga gttaaaccgt cgtggaatga tgcccaacca gctttagcac ctgcgtagaa
     1561 agtgttagct tgtggagctg cttgagctac tgcagctgct gataatactg ctaatgcaac
     1621 taatgttttt ttcattttga tgatcc
//
LOCUS       DANJWC010000235         1623 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.236, whole genome shotgun sequence.
ACCESSION   DANJWC010000235 DANJWC010000000
VERSION     DANJWC010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.236"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            178..>1623
                     /gene="xseA"
                     /locus_tag="PWX02_002350"
     CDS             178..>1623
                     /gene="xseA"
                     /locus_tag="PWX02_002350"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005619079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDL1730313.1"
                     /translation="MSNILTVTQLNYSVRHLLEMEMSHIWLTGEISNFSQPVSGHWYL
                     TLKDDKAQVRCAMFKMKNVRVNFRPQNGMQVLVRASVTLYEPRGDYQLIIESMQPAGE
                     GLLQQQFEQLKAKLSAQGLFAQEHKKPIPTFVKTVGIITSSSGAALQDILHILQRRDP
                     SLSVVIYPALVQGKEATQDIVNMIELANRRNECDVLIVGRGGGSLEDLWCFNEESVAY
                     AIYHSQIPIISAVGHETDVTIADFVADLRAPTPSAAAELVSRDQNELKRQLQHQFDKV
                     NLAFDRVWNHKFNRFQQLPLRLNAQHPSKQIQSQQQRFDYMEYRLEQAIYALVAAKKQ
                     VGKELNVRLIHQHPQKQIERQSQMLKQLELKLHWQMEQQLSKKQQKWKNITQRFERNP
                     LPYQLYQQQTMVAKMSQRLAYSIEKIMLKEQRHFQELCTKLDGLSPLKILTRGYSITQ
                     TKDGKTLTSTENIERGEAIITQLKTGKIISQV"
BASE COUNT          521 a          356 c          325 g          421 t
ORIGIN      
        1 ctggttcaaa tccggccccc gcaaccacat tccaaaccct cgatagtgaa tcacatcgag
       61 ggttttcgtt ttatttgcca aaaaacagca aaaaataacc gcttgttgac tgcaaaagct
      121 tcctctcgct ttcatttctc gttacacttc tactacattt acgctttttt gattattatg
      181 agcaatattt taactgttac ccaactcaac tattccgtcc gccatttgct cgaaatggaa
      241 atgagccata tttggctaac cggcgaaatc tcaaatttca gccaaccagt ttcggggcat
      301 tggtatctca ctttaaaaga tgacaaagca caagtgcgtt gtgcgatgtt taaaatgaaa
      361 aatgtaagag tgaattttcg cccacaaaac ggtatgcagg tgctggtgcg ggcaagtgtt
      421 acactctatg agccacgagg tgactatcag ctcattattg agagtatgca accggcgggc
      481 gaaggcttgt tacagcagca atttgagcaa cttaaagcaa aactttcagc tcaaggcttg
      541 tttgctcaag agcataaaaa gccaattcct acttttgtta aaaccgttgg tattattacc
      601 tcctcaagcg gagccgcatt acaagatatt ttacatattt tgcaacgacg agatccgagc
      661 ttatcagtgg ttatttaccc ggctctcgtg caaggcaaag aagcaacgca agatattgtg
      721 aatatgattg aactggcaaa ccgccgcaat gagtgtgatg tattaattgt cgggcgaggt
      781 ggcggttcat tagaggattt atggtgtttc aacgaagaga gtgtcgctta tgccatttat
      841 cattcacaaa tcccgattat cagtgcggtg ggacacgaaa ccgatgtgac cattgctgat
      901 tttgttgcag atttgcgcgc accaacgcct tcggctgctg cggaattagt cagcagagat
      961 caaaatgaat tgaaacgcca attacaacat cagtttgata aagtgaattt agcctttgac
     1021 cgagtttgga accataaatt taaccgtttc caacaactcc ccttacgcct gaatgctcag
     1081 catccaagca aacaaattca atcgcaacag caacgttttg attatatgga ataccgcctc
     1141 gagcaggcaa tttatgcact tgttgcagcc aaaaagcagg tggggaaaga gctaaatgtc
     1201 cgtttaattc accaacatcc gcaaaagcag attgaacgcc aatcccaaat gcttaagcaa
     1261 ttggagctga aactccattg gcaaatggag caacaactca gcaaaaaaca gcaaaaatgg
     1321 aaaaacatta cgcaacggtt tgaacgcaac ccgctacctt atcaacttta tcagcaacaa
     1381 acgatggtgg caaaaatgtc gcaacgcttg gcttattcta tcgaaaaaat aatgctgaaa
     1441 gagcagcgac attttcaaga gttatgcact aaacttgatg gacttagccc attgaaaatc
     1501 ttgaccagag gctactcaat tacccaaact aaagacggta aaacgcttac ttcaacagaa
     1561 aatattgaac gtggtgaggc tattatcacc caactaaaaa caggaaaaat aataagtcaa
     1621 gta
//
LOCUS       DANJWC010000236         1609 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.237, whole genome shotgun sequence.
ACCESSION   DANJWC010000236 DANJWC010000000
VERSION     DANJWC010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.237"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(25..1564)
                     /locus_tag="PWX02_002351"
     rRNA            complement(25..1564)
                     /locus_tag="PWX02_002351"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          351 a          501 c          333 g          424 t
ORIGIN      
        1 acttgctgtc gttctcgatt ttggtaagga ggtgatccaa ccgcaggttc ccctacggtt
       61 accttgttac gacttcaccc cagtcatgaa tcataccgtg gtaaacgccc ccctttcggt
      121 taagctatct acttctggta caacccactc ccatggtgtg acgggcggtg tgtacaaggc
      181 ccgggaacgt attcaccgca acattctgat ttgcgattac tagcgattcc gacttcatgg
      241 agtcgagttg cagactccaa tccggactta gacgtacttt ctgagattcg ctccccatcg
      301 ctggctcgcc gccctctgta tacgccattg tagcacgtgt gtagccctac tcgtaagggc
      361 catgatgact tgacgtcatc cccaccttcc tccggtttat caccggcagt ctcctttgag
      421 ttcccgacct aatcgctggc aacaaaggat aagggttgcg ctcgttgcgg gacttaaccc
      481 aacatttcac aacacgagct gacgacagcc atgcagcacc tgtctcatgg ttcccgaagg
      541 cactctcata tctctacaag attacatgga tgtcaagagt aggtaaggtt cttcgcgttg
      601 catcgaatta aaccacatgc tccaccgctt gtgcgggccc ccgtcaattc atttgagttt
      661 taaccttgcg gccgtactcc ccaggcggtc gatttatcac gttagctacg ggcaccagag
      721 ttaaacccca atccccaaat cgacagcgtt tacggcgtgg actaccaggg tatctaatcc
      781 tgtttgctcc ccacgctttc gcacatgagc gtcagtacat ttccaagggg ctgccttcgc
      841 cttcggtatt cctccacatc tctacgcatt tcaccgctac acgtggaatt ctacccctcc
      901 ctaaagtact ctagttgttc agtctgaaat gcaattccca ggttaagccc ggggctttca
      961 cacctcactt aaacaaccgc ctgcgtgccc tttacgccca gttattccga ttaacgctcg
     1021 caccccccgt attaccgcgg ctgctggcac ggagttagcc ggtgcttctt ctgtgattaa
     1081 cgtcaatcga ctgttctatt aaaacaatcg ccttcctcgt caccgaaaga actttacaac
     1141 ccgaaggcct tcttcattca cgcggcatgg ctgcatcagg gttcccccca ttgtgcaata
     1201 ttccccactg ctgcctcccg taggagtctg gaccgtgtct cagttccagt gtggctggtc
     1261 atcctctcag accagctaga gatcgcgggc ttggtgagcc tttacctcac caactaccta
     1321 atcccacttg ggctcatctt atggcaggtg gccaaatggt cccacccttt agtcctaaga
     1381 cattacgcgg tattagctac cgtttccagt agttatcccc ctccataagc cagattccca
     1441 agcattactc acccgtccgc cactcgtcac ccaaggagca aactcctatg tgctaccgtt
     1501 cgacttgcat gtgttaagcc tgccgccagc gttcaatctg agccatgatc aaactcttca
     1561 attcaaaaag tttaatcgct caatactgct gacataaaat cgcacttcc
//
LOCUS       DANJWC010000237         1596 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.238, whole genome shotgun sequence.
ACCESSION   DANJWC010000237 DANJWC010000000
VERSION     DANJWC010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.238"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            85..1536
                     /locus_tag="PWX02_002352"
     CDS             85..1536
                     /locus_tag="PWX02_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL1730314.1"
                     /translation="MKKIVSTLLILTSNLAFAETIPAQLQEKMDNQRMQENPTLEKPE
                     PQAKPQAVNAQAHRQSISISSQQLLQQPELLNSAMLTALVTNHTENVQFLLPIYQQLP
                     ASEQNSEVVQWAEAVNAKAKQNYSEAIKGYRQLLAKYPENQPIRMQLAVALFENRENE
                     AAEAQFYKLQAGKLPQEIANAVNSYLEAIGKQDQWSFQGGLTYLNDPNINNAPNAGTT
                     YGNWTAPKKESAQGVGFHFEADKKWSWGNGFFHEFRLNGNGKYYWNNKKYNEYSLRES
                     LGLGYQNAKQKITLLPFFEQMWYASGSSQTESTKRYSNAGGVNARWQYWITPQWQSAI
                     SYEYAEQRYTRRQHLDGNYHFIAPSLYFYPNSRQYWFVGANFNRTSPRDLDDSFIRRG
                     VSIGWGQEWKNGLSTQLSASYARKQYRAPMPIFQITQRNSEYGVQASIWHRALHFKGI
                     TPRLTWQYNKVKSNHSFYSYDKNRVYIEFSHTF"
BASE COUNT          524 a          367 c          320 g          385 t
ORIGIN      
        1 gaaaaatata gcaaaacaac ctcaaaactc ttaactaaag gctattgaaa aatagccttt
       61 tctattcaaa taaaagatag ctttatgaaa aaaatagtct caacgctttt aatccttacc
      121 tcaaacctgg catttgcgga aaccataccg gctcaacttc aagaaaaaat ggataaccag
      181 cgaatgcagg aaaaccctac attagaaaag cctgaacctc aagccaaacc acaagcggtt
      241 aatgcacagg ctcaccggca atcgatctct atttcaagcc aacagctgct tcaacagcct
      301 gagttgctca actcggcaat gctaactgcg ttggtcacta accacactga gaatgttcaa
      361 ttcttactgc ctatttatca acaattaccc gccagtgaac aaaacagcga agttgtacaa
      421 tgggcggagg cagttaatgc caaagcaaaa caaaattatt cagaggcaat aaaaggctac
      481 cgtcagctac tggcaaaata ccctgaaaat cagccaatcc gtatgcaatt agcggttgca
      541 ttgtttgaaa atcgggaaaa tgaagccgca gaggctcaat tttataagtt acaagcgggc
      601 aaattaccgc aagaaattgc aaatgcggtc aatagctatt tggaggcgat tggcaagcaa
      661 gatcaatgga gttttcaagg cggcttaacc tatttgaatg acccgaatat caacaatgct
      721 ccaaatgcag gcacgaccta tggcaactgg actgcaccga aaaaagaatc cgcccaaggg
      781 gtcggctttc actttgaggc ggataaaaag tggtcttggg gcaatggttt cttccacgag
      841 tttcgtttaa acggaaacgg caaatattac tggaataaca aaaaatataa cgaatattcg
      901 ctcagagaaa gcctcggctt gggttatcaa aatgcaaaac aaaaaatcac cttgttgccg
      961 ttttttgagc agatgtggta tgccagtggc tcaagccaaa ccgaaagcac caaacgctac
     1021 tccaacgcag gcggtgtgaa tgcccgctgg caatattgga ttactccaca gtggcaatct
     1081 gcaatcagct acgaatatgc ggaacagcgt tacactcgcc gccaacattt agacggcaat
     1141 tatcatttta ttgccccgag cctctatttt taccctaaca gccggcaata ttggtttgtg
     1201 ggggcgaatt ttaaccgcac cagcccccgt gatttagatg atagctttat tcgccgaggt
     1261 gtttctattg gttggggaca agagtggaaa aacgggcttt caacccaatt aagtgccagc
     1321 tacgcccgca aacaataccg agccccaatg ccgattttcc aaattacaca acgtaacagt
     1381 gaatatggtg tgcaagccag catctggcac agagccttgc attttaaggg tattacccct
     1441 cgcttaacgt ggcaatataa caaggtaaaa agcaatcata gcttctatag ttatgacaaa
     1501 aaccgtgtct atattgagtt ttcacatacg ttctaaacaa aaacgcccct taaacggggc
     1561 gatttcttag ttattttttc aatttacccc aacaat
//
LOCUS       DANJWC010000238         1585 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.239, whole genome shotgun sequence.
ACCESSION   DANJWC010000238 DANJWC010000000
VERSION     DANJWC010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.239"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..404)
                     /locus_tag="PWX02_002353"
     CDS             complement(<1..404)
                     /locus_tag="PWX02_002353"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601538.1"
                     /note="catalyzes the formation of S-adenosylmethionine
                     from methionine and ATP; methionine adenosyltransferase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDL1730315.1"
                     /translation="MAINLFTSESVSEGHPDKIADQISDAVLDEILKQDPKARVACET
                     YVKTGMALVGGEITTSAWVDIENLTRQVICDIGYTHSDMGFDAHSCAVLNAIGKQSPD
                     INQGVDRADPLEQGAGDQGIMFGYATNETEVL"
     gene            complement(587..1429)
                     /locus_tag="PWX02_002354"
     CDS             complement(587..1429)
                     /locus_tag="PWX02_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HDL1730316.1"
                     /translation="MKVGLVLEGGGLRGIFTAGVLDVFLDEHIHIDGAIGVSAGALFG
                     VNLPSKQKGRALRYNKKYAKDKRYMGLHSLLTTGNIVNRDFAYYELPSKLEPFDQQAF
                     EDSGMDFWVTVTNVETGEPEYIKIDNMFEQMEAFRATSSMPIVSKIVEIEGKKYLDGG
                     VSDSIPVQKFIEMGYDKIIVVLTRPLEYRKKPSSMWLFKRFYKKYPKLVQRWENRYAE
                     YNQAVEQIIQLNEKQQIFVIRPSRTVKISRLETDVNKIQAMYDLGVEDAKNALAGLRA
                     YLAK"
     gene            1489..>1585
                     /locus_tag="PWX02_002355"
     CDS             1489..>1585
                     /locus_tag="PWX02_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein"
                     /protein_id="HDL1730317.1"
                     /translation="MADAEFMDFLLDQLSPISGLRSKKMFGEYCLF"
BASE COUNT          433 a          352 c          295 g          505 t
ORIGIN      
        1 attaacacct cggtttcatt ggtagcgtag ccaaacataa tcccttggtc gcccgcacct
       61 tgctctaacg gatcggcacg gtcaacgccc tggttgatgt ccggagattg tttaccaatc
      121 gcatttaata ccgcacaaga gtgagcatca aagcccatat cagagtgagt atagccgatg
      181 tcgcaaatca cttggcgggt taagttttca atatccaccc acgcagaggt agtgatttcg
      241 ccccctacta atgccatacc ggttttcaca taagtttcac aagcaacgcg tgctttaggg
      301 tcttgtttta aaatttcatc taataccgcg tctgaaattt ggtctgcgat tttatccgga
      361 tgcccttctg aaaccgattc agaagtaaat aaattaattg ccatttgtat tcctctctat
      421 cttaaatatg gattattaat agtaggggcg ggtccagtgc ccgcccataa attatataaa
      481 atgtttggac aggcacagga ccggtcccta taaaatttgt ttggaagtgt ttccgtctag
      541 acgtctataa tatatttttt gaacgtttta ggcaaataga atttgtttat ttggctaaat
      601 atgcccttaa accagccaac gcatttttgg catcttctac ccctaaatcg tacattgctt
      661 gtattttatt tacatccgtt tcaaggcgac tgattttgac tgttctgctt ggacgaatca
      721 caaaaatctg ttgtttttca ttgagttgaa taatctgctc aacggcttga ttatattccg
      781 catagcggtt ttcccagcgt tgcactaatt tcggatattt tttataaaaa cgtttaaaca
      841 gccacatcga gcttggtttc ttacggtatt caagtgggcg agtaagcacc acgataattt
      901 tgtcatatcc catttcaata aatttctgca ccggaatact gtctgacaca ccaccgtcta
      961 aatatttttt accctcaatt tcgactattt tggatacaat cggcattgag gaagtagctc
     1021 gaaaggcttc catttgctcg aacatattgt caattttgat gtattcgggc tcaccggttt
     1081 cgacattggt tacagtaacc caaaagtcca taccggaatc ctcaaaggct tgttggtcaa
     1141 acggctccag tttggaaggg agttcatagt aagcaaaatc ccgattaacg atattgccgg
     1201 tggtaagcaa gctgtgtaac cccatatacc gcttatcttt agcgtatttt ttgttgtatc
     1261 gcaacgccct acctttttgt tttgaaggca aattcacgcc aaataacgca ccggctgaaa
     1321 caccgattgc tccatctata tgaatatgtt catctaaaaa tacatccaac acgccggcag
     1381 taaaaatacc tcgcaaacca ccgccttcca gcactaatcc aactttcata tcattctccc
     1441 tgtaatttgt gtattataac ccgaattatc ttgctaggag aaatcacaat ggctgatgca
     1501 gaatttatgg atttcctgtt agatcaacta agcccgattt ccggtttgcg ttcgaaaaaa
     1561 atgtttggtg aatattgctt gtttt
//
LOCUS       DANJWC010000239         1583 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.240, whole genome shotgun sequence.
ACCESSION   DANJWC010000239 DANJWC010000000
VERSION     DANJWC010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.240"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            217..522
                     /gene="cyaY"
                     /locus_tag="PWX02_002356"
     CDS             217..522
                     /gene="cyaY"
                     /locus_tag="PWX02_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HDL1730318.1"
                     /translation="MNTAQFHQAVEQVWQQIEEKIYDEGLSVDTEIQGAVCTLTFDDE
                     SQVIINKQEAMLELWLASKLGGFHFKNVEGEWVTDEGRSFWQHLEEAFARHGESVSF"
     gene            631..>1583
                     /locus_tag="PWX02_002357"
     CDS             631..>1583
                     /locus_tag="PWX02_002357"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HDL1730319.1"
                     /translation="MNTTAISVLNNIFGYQKFRDGQEEVINAVLENRDCLVIMTTGGG
                     KSLCYQVPALCLDGITLVISPLISLMKDQVDQLITNGIEAAYLNSTQTLEEQQLVEQK
                     ALSGQLKLLYLSPEKVMTQGFFHFISLCKISFIAVDEAHCVSQWGHDFRPEYTLLGGL
                     RNTFPNVPLMALTATADLTTRSDILHHLRLNSPHIYLGSFDRPNIRYTVQEKFKPLEQ
                     LIKFISKQQGKSGIVYCNSRKKVEEITEKLSARKISVMGYHAGMSFEQRERVQNAFQR
                     DDIQIVVATIAFGMGINKSNVRFVVHFDLPRSIESYYQETGR"
BASE COUNT          518 a          328 c          303 g          434 t
ORIGIN      
        1 aagattttat tttaaatagt aaaactaatt ttaaaagctc attacaggat gatagagcta
       61 ttcgttgtaa taatgaggat aaaataaaaa atcaagctaa acttaaaaag cctaattatt
      121 tcttaacaaa aatacaaaaa gaactttaca gaccatttaa acagctaaaa caaaaagtat
      181 ttactactta tataacattc aaataaggat acaccaatga acacagcaca attccaccaa
      241 gccgtagagc aagtatggca acaaattgaa gaaaaaattt atgacgaagg attatcagtc
      301 gatactgaaa tccaaggagc ggtctgcacc ttaacctttg atgatgaatc gcaagttatc
      361 attaataaac aagaggcaat gctggaatta tggcttgcca gcaaattggg tggtttccac
      421 tttaaaaatg tagaaggcga atgggtaaca gatgaaggtc gctcgttctg gcaacattta
      481 gaagaagctt ttgctcgaca cggagaaagc gtttctttct aatgcctaat tgttaactaa
      541 gacagcactc gtctttgatg agtgctttat tttcatttta aaagatactc tcagaagacg
      601 agtactatca taagtagcaa ccaactacca atgaacacca ccgcaatttc tgttttaaat
      661 aatatttttg gctatcaaaa attccgagac gggcaagaag aagttatcaa tgccgtgttg
      721 gaaaatcgtg attgcctcgt gattatgaca acaggcggtg gcaaatccct ttgctaccaa
      781 gtgccggctt tgtgcctaga cggcatcact ttggtgattt cgccgcttat ttcgttgatg
      841 aaagaccagg tggatcaact tatcaccaac ggcattgaag cagcttatct taactccact
      901 caaacgctcg aagagcagca acttgtggaa caaaaagcct tatccggtca attaaaatta
      961 ctctatcttt cccctgaaaa ggtaatgact caaggctttt tccactttat ttcgctatgt
     1021 aaaatcagct ttattgcggt tgatgaagcc cattgtgttt cccaatgggg gcacgatttc
     1081 cgccctgaat ataccctgct aggtggctta cgaaacactt tcccgaatgt gccgttgatg
     1141 gcactcaccg ccacggcaga tctaacaacc cgctccgata ttctgcatca tttacgctta
     1201 aatagcccac atatctattt aggcagcttt gatcgcccca atattcgcta caccgtgcag
     1261 gaaaaattta aaccgcttga acagctcatt aaattcatca gcaaacagca aggcaaaagc
     1321 ggtattgttt actgcaacag tcgcaaaaaa gtggaagaaa tcaccgagaa actctcagcc
     1381 cgtaaaatca gcgtgatggg ctatcacgcc ggtatgagtt ttgaacagag agaacgagtg
     1441 cagaatgcct ttcagcgtga tgatattcaa attgtggttg ccactattgc gtttggtatg
     1501 gggatcaaca aatccaacgt acgttttgtg gtacatttcg atttacctcg ctctatcgaa
     1561 tcttactatc aagaaaccgg acg
//
LOCUS       DANJWC010000240         1582 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.241, whole genome shotgun sequence.
ACCESSION   DANJWC010000240 DANJWC010000000
VERSION     DANJWC010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.241"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            3..1283
                     /locus_tag="PWX02_002358"
     CDS             3..1283
                     /locus_tag="PWX02_002358"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005762304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HDL1730320.1"
                     /translation="MSKATLTLETGEKLELNMQKGTLGYQTIDIQPFLKHKLFAYDPG
                     LVSTAVCQSNITYVDGDEGVLLYRGYPIDQLAKHSDYLEVGYMLLFGERPTKEEYKDF
                     VQLIKKHTLVHEQLTKFFSGFRRDSHPMAVMCGVSGALAAFYHDAIDVNNQAHRELTA
                     IRLLAKIPTLAAMCYKYSIGQPFMFPQNHLSYAGNFLYMMFATPCEPYEVNPVLERAL
                     DRIFILHADHEQNASTSTVRTAASSGANPFACIAAGIASLWGPAHGGANEACINMLEE
                     IGTVDRIPEFIARAKDKNDPFRLMGFGHRVYKNYDPRAKVMRETCHEVLKELNIDNPL
                     FEVALELERIALSDPYFIEHKLYPNVDFYSGIVLKAIGIPTSMFTVMFALARTVGWIA
                     HWKEMYLQGNIKIVRPRQIYTGHTKRDFEPMDKS"
     gene            complement(1356..1526)
                     /locus_tag="PWX02_002359"
     CDS             complement(1356..1526)
                     /locus_tag="PWX02_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HDL1730321.1"
                     /translation="MEYKGEQLKSNEDSKEKALIGNLWEKLSQGKGLFIMAVKSDENG
                     QDVRTQLLNKLA"
BASE COUNT          474 a          360 c          290 g          458 t
ORIGIN      
        1 ctatgtcaaa agcaacatta acactcgaaa caggtgaaaa acttgaactc aatatgcaaa
       61 aaggaacctt agggtatcaa acgattgata ttcagccttt cttaaagcat aagttatttg
      121 cttatgatcc cggattggtt tcgaccgctg tatgccaatc caatatcact tatgttgatg
      181 gcgatgaagg ggttttactc tatcgaggct acccgattga ccaattagcc aaacattctg
      241 attacttaga agtcggttat atgctgttat tcggcgaacg ccctaccaaa gaagaataca
      301 aagacttcgt tcaactcatc aaaaaacaca ctcttgtaca cgaacaacta accaaattct
      361 tctccggttt ccgccgagat tcgcacccaa tggcggtgat gtgtggggta agtggagctt
      421 tggctgcctt ctaccacgac gccattgatg taaacaacca agcccaccgt gaattaaccg
      481 ccattcgttt gttagccaaa atcccaaccc ttgcggcgat gtgttataaa tactctatcg
      541 gtcagccgtt tatgttccca caaaaccact tatcttacgc cggaaacttc ttatatatga
      601 tgtttgccac tccttgtgaa ccgtacgaag tgaaccctgt gttagagcgt gcgttagacc
      661 gaattttcat tttacatgca gaccacgaac aaaacgcttc gacctcaacc gtgcgaaccg
      721 ccgcttcatc aggtgcaaac ccatttgctt gcattgctgc cggtattgcc tctctctggg
      781 gacctgcaca cggaggagca aatgaagcct gtatcaatat gttggaagaa atcggcactg
      841 tggatcgtat tcctgaattt attgctcgag ccaaagataa aaatgacccg ttccgcttaa
      901 tgggctttgg gcatcgtgtc tataaaaact acgaccctcg tgcgaaagta atgcgtgaaa
      961 cctgccacga agtgttaaaa gaactgaaca ttgataaccc actatttgaa gtggcattgg
     1021 aattagagcg tattgcgtta agcgaccctt atttcattga gcataaactt tatccaaacg
     1081 tagatttcta ctccggaatc gtgctaaaag ccatcggtat tccaacctca atgtttaccg
     1141 taatgtttgc cctagccaga accgttggct ggattgctca ctggaaagag atgtatctcc
     1201 aaggcaatat caaaatcgtt cgtccacgtc aaatctacac cggacatact aaacgtgatt
     1261 ttgagccaat ggataaatca taagattagc aaaaaatcga aaaattttaa ccgcttgtgg
     1321 aaacaagcgg ttatattttt cataaaattt gcaaatcagg ctaatttatt tagcagttgt
     1381 gttctaacat cttgaccatt ttcatcgctt tttacagcca taataaataa gccttttcct
     1441 tgacttaatt tctcccaaag attgccaatt aaggcttttt ctttagaatc ttcattactt
     1501 tttaattgtt cccctttata ttccacaatt aaaattcgac catcaataag ctctacaata
     1561 aaatcagggt aaaaattctg at
//
LOCUS       DANJWC010000241         1575 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.242, whole genome shotgun sequence.
ACCESSION   DANJWC010000241 DANJWC010000000
VERSION     DANJWC010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.242"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..317
                     /locus_tag="PWX02_002360"
     CDS             <1..317
                     /locus_tag="PWX02_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL1730322.1"
                     /translation="SSELQNQINQLNQLRKQALKQEQDEQKLFEKNKIEDLPHFGTQL
                     NAQRKLLGIELTTLEMQTGISSSTLKRLFKDPSQVKFQTICIVAETLGFNLCAINTHK
                     QE"
     gene            complement(394..1413)
                     /gene="glpX"
                     /locus_tag="PWX02_002361"
     CDS             complement(394..1413)
                     /gene="glpX"
                     /locus_tag="PWX02_002361"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDL1730323.1"
                     /translation="MKRTLSFEFSRVTEAAALAAYSWLGRGDKNAADEAAVKAMRFML
                     NQMEIRGEVVIGEGEIDEAPMLYIGEKIGLSRLEDEEISIAVDPIDGTRMTAMGQANA
                     LSVLAAGGKETFLKAPDMYMEKLVVGQECKGMIDLNLPLEQNLRRVASKKGKLLSQLT
                     IAILAKPRHEKIIADVQKLGVRVIAIPDGDVATAVQCCLPESELDLLYGIGGAPEGVV
                     AGAAVRALGGDMQARLIPRNQVKGNSPENLTATEKELSRCNEMNVPVNTVLKLEDLVR
                     DDNIVFVATGITSGELLKGIKRRGNIASTETLLIRGKSRTIRKIQSDHYVDRKDNELL
                     SLLDL"
BASE COUNT          494 a          363 c          279 g          439 t
ORIGIN      
        1 tatcaagcga actacaaaat cagataaacc aactaaatca gttacgaaaa caggcactca
       61 aacaagagca agacgagcag aaattgtttg aaaaaaataa aattgaagat ttgccacatt
      121 ttggcacgca gcttaatgct cagaggaaac tattaggcat agaattaacc acccttgaaa
      181 tgcaaaccgg catttcaagc tctaccctaa aacgcttatt taaagaccca agccaagtga
      241 aatttcagac aatttgtatt gtggcagaaa cactcggctt taatttatgt gcaattaata
      301 ctcacaaaca agagtaagcg tgacaatgta gtgattttga ttactttggg aagacaaaaa
      361 atcgggctaa aagcccgatt tatcagagaa aagctataaa tccaataaag aaaggagttc
      421 gttatcttta cgatcgacat aatggtcgga ttgaatttta cgaatggttc tagatttgcc
      481 acgaataagt aaggtttcag tgctggcaat attgcctcta cgtttaatcc cttttaacaa
      541 ttcacctgaa gtaatgccgg ttgcaacaaa gacgatattg tcgtcacgca ccaaatcttc
      601 taatttcaac accgtattca ccggcacatt catttcatta cagcgtgaaa gctctttttc
      661 ggtagcagtt aaattttccg ggctattccc cttcacttgg ttgcgaggaa ttaaacgtgc
      721 ttgcatatcg ccacccaatg cacgaaccgc agcccccgcc actacccctt caggtgctcc
      781 gccgatacca taaagcagat ccaattcgct ctctggcaaa cagcattgca ccgcagtcgc
      841 aacatcgcca tcaggaatcg caatcactct tacccctaat ttttgcacat cggcaattat
      901 tttttcgtgg cgaggcttag caagaatagc aatggttaat tgggaaagta atttgccctt
      961 tttcgaggcg acacggcgaa gattttgttc aagcggaaga tttaaatcaa tcattccttt
     1021 gcattcctgc cccaccacta atttttccat atacatatca ggggctttta aaaaggtttc
     1081 tttgccgcct gctgctaata ccgacaacgc atttgcctgc cccattgcag tcatacgagt
     1141 accgtcaatc gggtcaaccg caatactgat ttcctcatct tctaggcggg atagaccgat
     1201 tttctcgcca atataaagca tcggtgcttc gtcaatttcg ccctcaccaa ttaccacttc
     1261 gccacggatt tccatttggt tcagcataaa ccgcattgct ttcaccgcag cttcatcagc
     1321 ggcattttta tccccacggc ctagccaaga gtaagctgct agagccgctg cttccgtaac
     1381 acgagaaaat tcaaaagaaa gagttcgttt catttttgtt ccttagtttc ttgaaagaaa
     1441 aactttaaaa gaagagagat tatattttat cagtaacctg caagctacgt tactaaaaaa
     1501 tcagcgtttg aataaaaatt cacaaatagc caaacttttt tataaaaata aagtcagatt
     1561 tagagtataa tcttt
//
LOCUS       DANJWC010000242         1532 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.243, whole genome shotgun sequence.
ACCESSION   DANJWC010000242 DANJWC010000000
VERSION     DANJWC010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.243"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..>1532
                     /locus_tag="PWX02_002362"
     CDS             <1..>1532
                     /locus_tag="PWX02_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HDL1730324.1"
                     /translation="KWDFIIIGGGASGLGIAVDAASRGYKTLLLESHDFAKGTSSRST
                     KLVHGGVRYLANGDVALVREALRERGRLAKNAAHLFKNQSFIIPNYKFKDCVMYRVGL
                     GLYDMLSGNLSLGKTENISKTEAKRRLPTLNDTGLRTGTVYQDGQFDDSRLAVNMAQT
                     VVEHGGTVLNHAKVTSLHKTPQGKIDGVTFYDELNQQEHTVFGTAVINATGVFMNDIL
                     SMDHGSTKKFVVPSQGIHLVLDKSFLPSNDALMIPKTSDGRVLFAVPWHEVLVVGTTD
                     TLIENPSYEPIPLEQEVNFILETAAQYLTKKPTRDDVKAVFAGQRPLAAPEKEGQSTK
                     EVSRSHKVLTDATSGLITITGGKWTTYRQMAEDTVEEALKVHSELGNKPCVTVNLPIH
                     GNIPAAQVNLQDHLYVYGSDIPQLRALMTETPEYAQKIHPRLDYTVAEVIWAVRHEMA
                     QTVEDVLARRVRLLFTDARAAIDSAAKVAAIMAKELGKDEMWQQQQVIQFLDVAKHYL
                     LV"
BASE COUNT          487 a          298 c          347 g          400 t
ORIGIN      
        1 aaaaatggga ttttattatt atcggtggtg gcgcaagtgg tttggggatt gcggtagatg
       61 cagcttcccg tggctataaa accttattat tagaaagcca tgactttgcg aaaggtacat
      121 cgagccgaag cacaaaatta gtccacggtg gcgttcgcta tcttgcaaat ggcgatgttg
      181 cactcgtgag agaagcattg cgtgaacgag gtcgcttagc taaaaatgca gctcatttat
      241 ttaaaaacca aagctttatt attccaaact acaaattcaa agactgtgta atgtatcgtg
      301 ttggtttagg cttatacgat atgctttcag gcaacctgag tttaggaaag actgaaaaca
      361 tcagtaaaac tgaagcgaaa agacgtttac caaccttaaa tgatactggt ttacgtacag
      421 gcactgttta tcaagatggg caatttgatg actcacgtct tgcagtgaat atggcacaaa
      481 cggtagtaga gcacggcggt acagtactaa atcacgccaa agtgacttca ctgcacaaaa
      541 cgccacaagg caaaattgat ggggtgactt tctacgatga actcaatcaa caagaacata
      601 ctgttttcgg cactgcggtg attaacgcaa ccggcgtatt tatgaatgat attttaagta
      661 tggatcacgg tagcacgaaa aaatttgtcg taccaagcca agggattcac ttagtattag
      721 ataaatcatt cttaccaagt aatgatgcgt taatgattcc aaaaacctct gacggacgag
      781 tgttatttgc tgtaccttgg cacgaagtgt tagtcgtagg gacaacagat actctgattg
      841 aaaatccttc ttatgagcca attccattgg aacaagaggt gaactttatt ttagagacag
      901 ccgcacaata tctgacaaaa aaaccaactc gtgatgatgt aaaagcagtc tttgccggtc
      961 aacgtccgct tgcggcaccg gaaaaagaag gtcaaagcac taaagaagta tctcgtagtc
     1021 acaaagttct cactgatgca accagcggtt tgattacgat tacaggtggt aaatggacaa
     1081 cttatcgtca aatggcagaa gataccgtag aagaagcgtt aaaagtccac tctgaactag
     1141 gcaataaacc ttgtgtgacc gtcaacttac cgattcacgg taatattccg gcagcacaag
     1201 tgaatctaca agatcatcta tatgtatatg gctcagatat tccacagtta cgcgccttaa
     1261 tgactgagac cccggaatat gcacaaaaaa tccatccgag actagattat accgttgcag
     1321 aagtgatctg ggcagttcgc cacgaaatgg cacaaacggt tgaggatgta ttagctcgcc
     1381 gtgttcgttt gctcttcact gatgcgagag cggcgattga tagtgcagca aaagtggctg
     1441 ctattatggc gaaagaatta ggtaaagatg aaatgtggca acaacagcaa gttatccagt
     1501 tccttgatgt tgcgaagcac tatttattgg ta
//
LOCUS       DANJWC010000243         1520 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.244, whole genome shotgun sequence.
ACCESSION   DANJWC010000243 DANJWC010000000
VERSION     DANJWC010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.244"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..728
                     /locus_tag="PWX02_002363"
     CDS             <1..728
                     /locus_tag="PWX02_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase family
                     protein"
                     /protein_id="HDL1730325.1"
                     /translation="DSILKPKFIINFGKKQKSIYNLDTVKNYDFTTVFPKQVHFADYL
                     NYAIFADFLEKNQVNYNLNIPNELSSFANTLWQEKKIRLLLNPQGSMREISTQELSEL
                     LSMIDKRYVQHISCLLTNTTNSAEYLSKIKAPFEISLAPNSDILGYCALVNSADIVIA
                     VDGGGVHISCALGKKLLSFYANHPANIARWYPKPKKDVETFMVIADKWTEDNNDTRGF
                     SLVAPAKWLNHQFAKLIEKYTAC"
     gene            complement(783..995)
                     /gene="rpmE"
                     /locus_tag="PWX02_002364"
     CDS             complement(783..995)
                     /gene="rpmE"
                     /locus_tag="PWX02_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDL1730326.1"
                     /translation="MKQGIHPEYKEITATCSCGNVVKTRSTVGKDLNLDVCGNCHPFY
                     TGKQRVVDTGGRVERFNKRFSIPSSK"
     gene            1358..>1520
                     /locus_tag="PWX02_002365"
     CDS             1358..>1520
                     /locus_tag="PWX02_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730327.1"
                     /translation="MTERKVLANAIRFLSMDAVQKANSGHPGAPMGMADIAEVLWRDF
                     LKHNPTNPKW"
BASE COUNT          515 a          263 c          257 g          485 t
ORIGIN      
        1 tagatagtat tttaaagcct aaatttatta ttaacttcgg taaaaaacaa aaaagtattt
       61 acaatttaga tacagtaaaa aattacgatt tcaccactgt ctttccaaag caggttcatt
      121 ttgctgacta tctcaactat gctatttttg ctgatttttt agaaaaaaat caggtcaatt
      181 ataatttaaa cattcctaat gaactaagca gttttgcaaa tacgctatgg caagagaaga
      241 aaattcgatt actattgaat cctcaaggta gtatgcgtga aatttctact caagaattat
      301 cagagctatt atctatgatt gataaaagat atgttcaaca tattagttgt ttgctgacca
      361 atacaaccaa tagtgccgaa tatttgtcga aaattaaagc cccatttgaa ataagcctag
      421 cacctaatag tgatatttta ggttattgtg cattagtaaa ctctgctgat attgtaattg
      481 cagttgatgg aggtggtgtc catatttctt gtgcattagg caagaaattg ctctcatttt
      541 atgccaatca tccagctaat attgcacgct ggtatccaaa acctaagaaa gatgttgaaa
      601 cttttatggt gatcgcagat aaatggaccg aggataataa tgatactcga ggtttttctc
      661 tggtagctcc tgcaaaatgg ttaaatcacc aatttgcaaa attgatagaa aaatacaccg
      721 cttgttaatt cgatatataa gaaaaacaaa accccgcatt agcggggttt gcaataatca
      781 aattattttg agcttgggat gctgaaacgt ttgttaaagc gttcaacacg accaccggtg
      841 tcaacaacac gttgtttacc agtatagaac gggtggcagt tgccacatac gtctaagttt
      901 aaatctttac ccactgttga gcgagtttta actacattac cgcaagagca agttgccgta
      961 atttctttat attctgggtg aataccttgt ttcattgggg aacctcataa gaagccatat
     1021 cgccgcctat cttgttagaa tctctaccgc tgataggtac catatgtaat taataaaaat
     1081 gttgagcaaa tgctcaacgc caaaaagagt gcgtatttta gataaatttg ttgcctagat
     1141 caactactaa acattcgctt tatcaagaaa atattgtatt aggaaaaaac cgtctattaa
     1201 aatcaaatcc aatcaaaaca aatcacaatt taatcatttt ttgtgataca gatcacattt
     1261 ctcatttcca tttttgagtt tttctagcaa aatagtgtag aatccaattc gatatccttt
     1321 ccattttata taacattctc gattaggagt aacacaaatg acagaacgta aagtgctagc
     1381 gaatgcaatt cgttttttaa gtatggatgc tgtacaaaaa gctaactcag gccacccagg
     1441 cgcaccaatg ggtatggctg atattgcaga agttttatgg cgtgattttt taaaacacaa
     1501 tccaactaat cctaaatggg
//
LOCUS       DANJWC010000244         1486 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.245, whole genome shotgun sequence.
ACCESSION   DANJWC010000244 DANJWC010000000
VERSION     DANJWC010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.245"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..1099
                     /locus_tag="PWX02_002366"
     CDS             <1..1099
                     /locus_tag="PWX02_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005705532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730328.1"
                     /translation="ESVIASLFDFGVIGIHLDTGEIQQGIRTGKYRHKGSYCDEVSIQ
                     VSGSTIKMEGNPSRWGKTENLLGFTSIDACVHCFNNILAKLKLPLFTRCTEIFHGQGK
                     DGEKVHTFSNGAIIKRLDITTNKSVGKGNERTFIKALSQMRYRNSIGRLHTNGCTADW
                     LSAKGNANLIYPSCYIKHEELRIHSYDKIKNKFGEHSSEFQYYKKVYDYCEQNGVVRF
                     EQKLKSRYLKRENLCYWGLSDFSKLEQLQEEFINMHKNLGVSKIELQTIAEQLVNKGV
                     VDTLRKANTTAFYAMKWASGEDMNLLPERTFKRHRSLLRKIGIDIANPCDVEKFQAVQ
                     VISCEQIFVRPFKAPDFYQYPSNTPILRLVA"
     gene            1126..1461
                     /locus_tag="PWX02_002367"
     CDS             1126..1461
                     /locus_tag="PWX02_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005562620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL1730329.1"
                     /translation="MRTGFYIVGVLKGYKASTFTNRDTGEVKDKHNIGIQLQDPDGYG
                     GYNTTTQEIKVDERCINEGFKRTIQQHKDKLVMILVYPREWAMDGGRKGITYNFDENS
                     MIQPIDENK"
BASE COUNT          523 a          276 c          287 g          400 t
ORIGIN      
        1 ggaatccgtg attgcttcgc ttttcgattt cggtgtgatt ggcattcatc ttgataccgg
       61 ggaaattcaa cagggcatta gaacaggcaa gtatcgtcac aaaggcagtt actgtgatga
      121 ggtatccatt caggtatccg gctcaactat aaaaatggaa ggtaacccaa gccgttgggg
      181 taaaactgaa aatttactcg gctttacatc tatagatgct tgcgtacatt gttttaataa
      241 cattcttgct aaacttaaac taccgctttt tactcgctgt acagagattt ttcacgggca
      301 gggtaaagac ggagaaaaag tacatacctt ttcaaacggt gcaatcatta aaagattgga
      361 cattaccact aataaatctg ttggtaaggg taacgaaaga acctttatca aagcattatc
      421 acaaatgcgg tatcgcaatt ctatcggacg tttacacact aatggctgca cagctgattg
      481 gttgagtgca aaaggtaacg caaatcttat ttatccctct tgctacatca aacacgaaga
      541 attacgaatc cactcttacg acaaaatcaa aaataaattt ggcgaacatt catcagaatt
      601 tcagtattac aaaaaagtgt acgattactg cgagcaaaat ggggtagttc gttttgagca
      661 aaaactaaaa tcacgctact taaaaagaga aaatctttgc tattgggggc ttagcgattt
      721 ttctaaatta gagcagttac aagaggagtt catcaatatg cacaaaaact taggcgttag
      781 caaaatagag ctacaaacga tagcagaaca gcttgtaaat aaaggggtag ttgacacact
      841 tcgtaaagcg aatactactg cattttacgc aatgaaatgg gcaagcggcg aagatatgaa
      901 tttattacct gaaagaacat ttaaacggca tcgttcatta ttaagaaaaa tcggcataga
      961 cattgctaac ccttgtgatg tggaaaaatt ccaagctgtt caagttatat cctgcgaaca
     1021 aatctttgtt cgaccgttta aagcacctga tttttatcaa tacccgagca acacgcctat
     1081 tctgcgtttg gttgcttaat catcaactaa caagaggaaa ttactatgcg tacaggcttt
     1141 tacattgttg gtgtacttaa aggctacaaa gccagcacat tcactaatcg agataccggc
     1201 gaagttaaag ataagcacaa tatcggcatt caacttcaag accctgacgg ttacggcggt
     1261 tacaacacca caacacagga aatcaaggtt gatgaacgtt gcattaacga aggatttaaa
     1321 agaaccattc aacaacacaa agacaagctg gtaatgatct tagtttaccc acgtgaatgg
     1381 gcaatggacg gcggacgtaa aggtattaca tataactttg atgaaaattc aatgatacag
     1441 ccaattgatg aaaataaata aataaataaa tccttataaa tcaata
//
LOCUS       DANJWC010000245         1449 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.246, whole genome shotgun sequence.
ACCESSION   DANJWC010000245 DANJWC010000000
VERSION     DANJWC010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.246"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            113..1156
                     /gene="msrB"
                     /locus_tag="PWX02_002368"
     CDS             113..1156
                     /gene="msrB"
                     /locus_tag="PWX02_002368"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005546455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDL1730330.1"
                     /translation="MKYNQLLLCLVSAIFTTSSIANITTPNEMRSENLQDIYLAGGCF
                     WGIEAYMEKIYGVKEATSGYANGKTEQTAYRIIGQTDHAETVHVTYDANKISLEKLLQ
                     YYFKVIDPTSKNKQGNDIGRQYRTGIYYQNEQDKQIILKEIQAQQAKYKQKIQVQVEP
                     LKHYILAEEYHQDYLKKNPNGYCHIDLNQANSIIIDPKDYPKPSDATLKMKLTPLQYS
                     VTQRKNTERSFSNEYWDNTAPGIYVDITTGEPLFSSLDKYDSGCGWPSFTKPIINEVV
                     NYTEDNSFNMTRTEVLSRSGNAHLGHVFTDGPKDKGGLRYCINSASIKFIPLEQMEKE
                     GYGYLINMIKGNK"
     gene            1186..>1449
                     /locus_tag="PWX02_002369"
     CDS             1186..>1449
                     /locus_tag="PWX02_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HDL1730331.1"
                     /translation="MMLSLNSYAQENSHLGHIPLKDLNQNQITLDKYQGKKIYIKAWA
                     SWCSICLAGLAEIEEISQKANNDIEIITIVSPGHKGEKTTNQFI"
BASE COUNT          560 a          243 c          250 g          396 t
ORIGIN      
        1 tagagagtaa aaccaatttt gatcttagca aaaaagtaag aaattatgaa tgtttaaggt
       61 taaaccctcg tccttaaaaa ataaaattac ataagttata aaggagaact gaatgaaata
      121 caatcaactg ttactatgtt tggtgtctgc tatctttacc acctcctcaa tagcaaacat
      181 cacaacccct aacgagatgc gatctgaaaa cttacaggat atttacttgg caggaggatg
      241 tttctggggc attgaagcct atatggaaaa aatatatggc gtaaaagagg ctacatcggg
      301 ctatgccaat ggcaaaactg agcaaactgc ttaccgaatt atcgggcaaa ccgatcatgc
      361 agaaacagtt cacgtgacct atgatgctaa taaaatttct ttagaaaagc tgttgcaata
      421 ttactttaaa gtaattgatc caaccagtaa aaataaacaa ggtaatgata ttggccgcca
      481 atatcggaca ggtatttatt atcagaatga gcaagataaa caaattattt taaaagaaat
      541 acaagctcaa caagcaaaat acaaacaaaa aatacaagtg caagtagaac cattaaaaca
      601 ttatattttg gcagaagagt atcatcagga ttacctaaag aaaaatccaa atggctactg
      661 ccatattgat ttaaaccaag ccaacagcat tattatcgat ccgaaagatt atcctaaacc
      721 tagtgatgca acattaaaaa tgaaattaac gcctttgcaa tatagcgtta cccaacgtaa
      781 aaatactgag cgttcattca gcaatgaata ttgggataat accgcaccgg gcatttatgt
      841 tgatataact acaggagagc ctcttttttc atctctcgat aaatatgact ccggctgtgg
      901 ctggccaagt tttaccaaac caattatcaa tgaggttgtt aactataccg aagataatag
      961 ttttaatatg acaagaacgg aagtactaag tcgaagtggt aacgctcatt taggacacgt
     1021 tttcactgat ggcccgaaag ataaaggcgg attacgttat tgtatcaaca gtgcctcaat
     1081 caaatttatt ccattagagc aaatggaaaa agaaggttac ggctatttaa taaatatgat
     1141 taaaggaaat aaataatgat gaagaaaata attagtttac tcataatgat gttgagtctt
     1201 aacagctatg cacaagaaaa ctctcatctt gggcatattc ctcttaaaga tctcaatcaa
     1261 aatcaaatta ctcttgataa atatcaaggt aaaaaaatct atataaaagc atgggcatct
     1321 tggtgttcta tttgtctagc cggattggcg gaaattgagg aaataagcca aaaagcaaat
     1381 aacgatattg aaattattac tattgtttca ccagggcata aaggcgagaa aacaaccaat
     1441 caatttatt
//
LOCUS       DANJWC010000246         1429 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.247, whole genome shotgun sequence.
ACCESSION   DANJWC010000246 DANJWC010000000
VERSION     DANJWC010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.247"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            79..666
                     /locus_tag="PWX02_002370"
     CDS             79..666
                     /locus_tag="PWX02_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SocA family protein"
                     /protein_id="HDL1730332.1"
                     /translation="MFDELKIAQATAYLLHKAGGTMEHIKVMKLLYLADRLSWQRYGY
                     SISNDEYYSLPYGPVLSNTLNLMRGEEASLYQKTSIWGEWIADKEDHKISLKKSVNTE
                     DEYFFDCLSESDTEILEEVFATFGNYKPFALVEYTHNPKYIPEWEDPQGRSKPISLNT
                     LLTKLGKTPSEIESILEQQTEKGEMDNLFKGLINA"
     gene            659..1107
                     /locus_tag="PWX02_002371"
                     /pseudo
     CDS             659..1107
                     /locus_tag="PWX02_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252950.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
BASE COUNT          515 a          273 c          253 g          388 t
ORIGIN      
        1 gtatttgaac aaatcgcacg tctttaaaat attaaattaa gagaaagcac tatttgcgat
       61 gtagcaggag ataaaacaat gtttgatgaa ttaaaaatag cccaagcaac cgcctatttg
      121 ctacataagg caggcggaac aatggagcat attaaggtaa tgaaattgct ttatcttgcg
      181 gatcgcttat cgtggcaaag atacggctat tctatttcta atgatgaata ttattcattg
      241 ccttatggac cggtattaag caacacactc aacctaatgc gaggagaaga agcgagctta
      301 taccaaaaaa ccagtatttg gggcgagtgg atagcagata aggaagatca caaaatcagc
      361 cttaaaaaat ccgtaaatac cgaagatgaa tacttctttg attgcctttc agaaagcgac
      421 accgaaattt tagaagaggt atttgcgacc tttgggaatt ataagccatt cgccctagtg
      481 gaatataccc ataaccctaa atacattccc gaatgggaag acccacaagg cagaagcaag
      541 ccgatttctt taaacacgct tcttactaag ctagggaaaa cgccaagcga gattgaaagc
      601 attttagagc aacagacaga aaaaggagaa atggataacc ttttcaaggg cttaatcaat
      661 gcttgaagtc aaaagacaag caactttctt agaccgacca actacggcaa ggtagagaac
      721 tcctacaagc atttatatat tctgcttacc aatccttgtt ttgatggaga aaccggtaaa
      781 ggagatatga ttttaagtgt aaactgctca agcattaaaa acgaaaaagc acttgatgaa
      841 acttgtattt taaaggcagg aacacacgaa tttattacac gggacagctt cattttctac
      901 cgccatataa aaattgattc gttagatgag attaaggcaa atatagaagc cggttatttt
      961 attcaaaaag ccctaattaa tgatgaagcc tatcaacaaa tactaaaagg cgtgctatcg
     1021 tcaaaatcgg taacgcctag atataaacgc tttctaaaaa atgcaatccg ccaaagtgct
     1081 tgccctgata ttctagccga accttaagcc gttgatgaaa attagcgact ttttgccctt
     1141 taaccactac caaaaccgct aatatttccc ctctaaaagt ggctaacagt gggtaaaaat
     1201 caaagttacc cacttaaccc actgtaaccc acttgcttcc cactggctat aaagccttat
     1261 atatcaaggc ttccaacaaa tcagtgggta aaatttcaaa aagttttaaa ttttttcttt
     1321 tattcaaaaa taaaatttcc caagaactaa tttttagcta cggcgatagc tcgaaatatc
     1381 tgtttataaa ataacctaat taataaggca ctttggattt tagttatat
//
LOCUS       DANJWC010000247         1370 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.248, whole genome shotgun sequence.
ACCESSION   DANJWC010000247 DANJWC010000000
VERSION     DANJWC010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.248"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..1326)
                     /locus_tag="PWX02_002372"
     CDS             complement(<1..1326)
                     /locus_tag="PWX02_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDL1730333.1"
                     /translation="MKLTKIALSVVAVISAVAVGGSWYTGKQVEEKYQELVVQANNQL
                     KQLSGEFGANIELKDVQIDRHFFSSNAKYRLEVDLGNGKKLDFVGNDKLHHGPLPLNR
                     LAKLNLAPVMMSAESKLQAPEALKKLMDEHIGTGFSNISYSGDVEGHFDISPIKHSDE
                     TGHIETTPIKVQYSYDQKLKGVDSTVELESLKIKAEEFDFQIQGIVYDVQTENNESYP
                     NLTLGKGSGKINAIEFKSKDGKLSQIKDVVVKGDNLLKGDRLISTGDLEAATFNIEGV
                     EMGKLKMDMAYDLDAKLTNDITPLLSNPQTLENEKTGELLLQLLAKSFKFHINNFSLE
                     NSKGKVDLALLLNMAQFDPQNLGNMQAVLQALSTSKFTSNINRQYAEDIIRQVSIVTE
                     KLGEEEAKAFAKQQVDAVFLNAGVEQYGLRKVDDNNVKIELTIDNGKVNL"
BASE COUNT          402 a          276 c          196 g          496 t
ORIGIN      
        1 taaattcact tttccgttat ctatagttaa ttcaatcttc acattgttgt catctacttt
       61 tcttaatccg tactgttcaa ctcccgcatt gagaaaaaca gcatcaactt gttgcttggc
      121 aaatgctttc gcttcttctt cgcctaattt ttctgtaact attgagactt ggcgaataat
      181 atcttccgca tattgacggt tgatatttga agtgaattta cttgttgaaa gggcttgcaa
      241 taccgcttgc atattaccaa gattttgtgg gtcaaattga gccatattca gtaataaagc
      301 caaatcaact ttgcctttgc tgttttcaag tgagaaatta ttaatatgga atttaaatga
      361 ttttgctaat agttgtaata ataactcgcc tgttttttca ttttctaatg tttgaggatt
      421 agataataaa ggcgtaatat cattggttaa tttagcatct aaatcgtatg ccatatccat
      481 ttttaattta cccatttcaa caccctcaat gttgaaagtg gctgcctcta aatctccggt
      541 agaaattaaa cgatcgcctt ttaatagatt atcgcccttc accacaacat ctttaatttg
      601 agataatttg ccatctttag acttaaactc aatagcgtta atttttccac tacctttgcc
      661 taaagttaaa ttaggataac tttcattatt ttctgtttga acatcataaa ctataccttg
      721 aatttgaaaa tcaaattctt ctgcttttat cttaagactt tctagctcaa cagtgctatc
      781 aacccctttt aatttttggt cgtagctata ttgcactttg attggcgtgg tttcaatatg
      841 acctgtttca tcactatgtt taatcgggga aatatcaaaa tgcccttcca catcgcccga
      901 atagctgata ttagaaaaac cggtgccaat atgttcatcc atcaattttt taagggcttc
      961 aggagcttgt aatttgcttt ccgcactcat cattacagga gctaaattaa gtttcgctaa
     1021 acgattaagc ggtagcggac cgtgatgaag tttatcatta ccaacaaagt ccaatttctt
     1081 accattaccc aaatccactt ctaaacgata tttcgcatta gagctaaaga aatggcgatc
     1141 gatctgcaca tctttaagct caatattcgc accaaattcg ccactcagtt gtttaagttg
     1201 gttattagct tgaacaacca attcttgata tttttcttca acctgcttac ctgtgtacca
     1261 acttccgcca actgcaacag cactaattac cgctacaact gaaagagcaa tttttgttaa
     1321 tttcattctg tttcctttta ttttcaaaaa aatttaaaaa atgaccgctt
//
LOCUS       DANJWC010000248         1359 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.249, whole genome shotgun sequence.
ACCESSION   DANJWC010000248 DANJWC010000000
VERSION     DANJWC010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.249"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            29..407
                     /gene="rnpB"
                     /locus_tag="PWX02_002373"
     ncRNA           29..407
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PWX02_002373"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            468..1106
                     /locus_tag="PWX02_002374"
     CDS             468..1106
                     /locus_tag="PWX02_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1007 family protein"
                     /protein_id="HDL1730334.1"
                     /translation="MKFIKTIITLIALYSMPVFSHPHSFVDLKTNVIVEGSMLKSFQM
                     EWMLDEIASSELIYEVKNSQDKEKTQQNITAEMVQTALQNHYFSTLYDSQNQPIKFTT
                     KPTATRFEIKNNRVIFYITFHLAKPHELKQSKVRFYTYEPSYYIAMEYNRPADVNISN
                     ASCKATLVQPQVDSKLRLYASGLDKNQSPDMPENGDYSLGVQFAQKVEIICD"
     gene            1097..>1359
                     /locus_tag="PWX02_002375"
     CDS             1097..>1359
                     /locus_tag="PWX02_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt transporter"
                     /protein_id="HDL1730335.1"
                     /translation="MRLKATYWGMIVIGFLSLFALYQLYPMILFKVMEWQKNFNLQLS
                     ASLNELAENQSKAGITLVFISFLYGVFHAVGPGHGKFILSSYLS"
BASE COUNT          429 a          232 c          298 g          400 t
ORIGIN      
        1 tttttgcgaa agcattatac tctagtgctg agttggttag gcaatcgctg gtttattgaa
       61 gcctctaaat tcgtgaaaac gtaaactggg ggacaagtaa acgagaggaa agtccgagct
      121 acacagggca gagtgccgga taacgtccgg ggggcgtgag cctacgacca gtgcaacaga
      181 gagcagaccg ccggctttgt cggtaagggt gaaagggtgc ggtaagagcg caccgtgtgg
      241 gtggtaacat tccacagcag ggtaaactcc actcgtagca agaccaaata ggaactcaat
      301 cggtggcccg ctgagagttc gggtaggttg cttgagcgta tgtgtaaatg tacgtctaga
      361 ggaatgattg tccacgacag aactcggctt atcgaccaac tcactaaatt cttgaaatgc
      421 gaacaggggg aacttgttcg cttttttatt ttatagtgaa agtaaatatg aaatttatta
      481 agacgattat cacgttaatt gcattatatt caatgccagt tttttctcac ccacattcat
      541 ttgttgattt gaaaacgaat gttattgtgg aaggctcaat gttgaaatct ttccaaatgg
      601 aatggatgct agatgaaatt gcttcttctg agttaattta tgaagtgaaa aatagccaag
      661 ataaagaaaa aactcagcag aatatcactg cagaaatggt gcaaaccgca ttgcaaaatc
      721 attattttag caccttatat gatagtcaaa atcagccgat aaaatttacg acaaagccca
      781 cagcgactcg ctttgaaatc aagaataaca gagtgatttt ttatattact tttcatttag
      841 ctaaacctca cgaattaaag cagagtaaag tgagattcta tacttatgaa ccgagttatt
      901 atattgcgat ggaatataat cgccctgctg atgtcaatat cagtaatgca agttgtaaag
      961 caacattggt gcaacctcaa gtagatagta agttacgctt atatgcttcc ggtttggata
     1021 aaaaccaatc accggatatg cctgaaaatg gagattactc tcttggtgtc caatttgctc
     1081 aaaaagtgga gattatatgc gattaaaagc aacatattgg ggaatgatag tgataggatt
     1141 tttgagccta tttgctcttt atcagcttta tccaatgata ttgtttaaag tgatggaatg
     1201 gcaaaaaaac tttaatttac agctttccgc ttcgctcaat gaattagctg aaaatcaaag
     1261 taaagccggt attaccttag tatttattag ttttttatat ggagtatttc acgcagttgg
     1321 gcctggtcac ggaaaattta ttctgagcag ttatctttc
//
LOCUS       DANJWC010000249         1353 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.250, whole genome shotgun sequence.
ACCESSION   DANJWC010000249 DANJWC010000000
VERSION     DANJWC010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.250"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            2..>1353
                     /gene="dacB"
                     /locus_tag="PWX02_002376"
     CDS             2..>1353
                     /gene="dacB"
                     /locus_tag="PWX02_002376"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDL1730336.1"
                     /translation="MTQAEINTEELLNTLPAGTSVSFIAKNLDTNQIITQYQSDIFML
                     PASTQKVFTALAAKLTLTDDFRFQAALLSNGKVENGVLKGSLIARFSGDPELTSGQIY
                     QLISKLKQQDINKIEGDLILDTSVFASHDKASGWIWNDLTMCFNAPPAAINVDHNCFY
                     VTLNADQPIGEFAKVNVPSAYPVQVFSSAYIVEPKEAPFCQLDVVVHDNNRYQIKGCM
                     ARQSQPFGLSFSVQDPTNYGANIIKAHLKSLKIAFNGQVKESLTAQNGTLLAEHYSEP
                     LPVLLKKMMKKSDNQIADALFRTVANKQHNRPASFQLGSYVIRRLLKTKANIDFKNSV
                     VTDGSGLSRHNQVSSRTMLESLEYIAQNEESLKLFETFPIAGVDGTISGRGSISTEPL
                     AKNLIAKTGSLKGVYNLAGFMKNARGERIAFVQFINGYSTGELESKTKRAPLNTFENK
                     "
BASE COUNT          415 a          306 c          275 g          357 t
ORIGIN      
        1 tattacccag gctgaaatta ataccgaaga attattaaat accttgcctg ccggcacttc
       61 tgttagcttt attgctaaaa atttagacac taaccaaatc atcactcaat atcaaagtga
      121 tatttttatg ctacctgcca gcacccaaaa agtctttacg gcattagcag ccaaattaac
      181 cttaaccgat gatttccgct ttcaagctgc ccttttaagt aacggaaaag tagaaaacgg
      241 ggtgttaaaa ggcagtttaa ttgcccgttt tagtggcgac cctgagctga caagcggtca
      301 aatttatcaa ttaattagca aattgaagca acaagatatc aataaaattg aaggggattt
      361 aattttagac acttcggttt ttgccagcca cgacaaagcc tccggttgga tttggaacga
      421 cttgacgatg tgctttaacg ctccaccagc ggcaattaat gtagatcaca actgttttta
      481 tgtcacttta aatgcagacc agcctattgg cgaatttgct aaagtgaatg tgccgtctgc
      541 ctacccagtg caggtgttta gctctgctta tattgtcgag ccgaaagaag ccccattttg
      601 ccagcttgat gtagtggttc acgataacaa ccgttaccaa atcaaaggct gtatggctcg
      661 ccagtctcag ccgttcggct taagtttttc ggtgcaagat ccaaccaatt acggagcaaa
      721 tatcattaaa gctcatttaa aaagcctgaa aattgcgttt aacgggcaag taaaagagtc
      781 attaaccgcc caaaacggaa cgctccttgc cgagcattat tccgagccgt tgccggtgtt
      841 gctgaagaaa atgatgaaaa aatcggataa ccaaattgcc gatgccctgt ttagaaccgt
      901 tgccaacaag cagcacaacc gccctgcctc attccaatta ggcagttatg tgattcgtcg
      961 actattaaaa accaaagcca atattgactt taaaaacagt gtggtgaccg atggctcagg
     1021 gctttcccgc cataatcaag tgagtagccg aacaatgctg gaaagccttg aatatatcgc
     1081 ccaaaacgaa gaaagcttaa aactgtttga aaccttcccg attgcaggtg tagatggtac
     1141 tatttccggc agaggctcaa tttcaaccga accgcttgcc aaaaacctga ttgctaaaac
     1201 cggctcactg aaaggggttt acaaccttgc cggctttatg aaaaatgccc gtggcgaacg
     1261 catcgctttt gtgcaattta ttaacggcta ttccactggc gagctggaaa gcaaaaccaa
     1321 acgggctccg cttaatacgt ttgaaaataa att
//
LOCUS       DANJWC010000250         1317 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.251, whole genome shotgun sequence.
ACCESSION   DANJWC010000250 DANJWC010000000
VERSION     DANJWC010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.251"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(208..501)
                     /locus_tag="PWX02_002377"
     CDS             complement(208..501)
                     /locus_tag="PWX02_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730337.1"
                     /translation="MTTFNKILNPMYSTIASYSTQDDGSLNAKYVVGTGDDTDGEVTN
                     FVIITSEYKYIDAQSAKAITDAPLTKEDIGKTPTQIMLGRIYKYLKETGQIVV"
     gene            645..1034
                     /locus_tag="PWX02_002378"
     CDS             645..1034
                     /locus_tag="PWX02_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL1730338.1"
                     /translation="MADPIEWSIITTDCFDEWFGQQDESTQERVLACLIYLRKFGYSL
                     GRPYADTVYQSKFPNMKELRIQNKGKPIRAFYAFDPLRQAIVLCAGEKGKDKRFYEKM
                     IALADAEFTSYLNNLEKENENVRSNAQ"
     gene            1006..1305
                     /locus_tag="PWX02_002379"
     CDS             1006..1305
                     /locus_tag="PWX02_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL1730339.1"
                     /translation="MKTLDQMLNELSPERRQKVEDMAQEMIREIQLSKLREELNLSQQ
                     QLATALGISQPAIAKLEQKDNDPRLSTLKRYIEAMGGKLKLAVELPTGDERVIRI"
BASE COUNT          427 a          235 c          239 g          416 t
ORIGIN      
        1 attataaata aaacgttccg aaaaaacatt gaatttcatt aaaaaattcc tttatttatc
       61 aaataaatgc gatttgtttc ggaacgtttt ttaatgctta ttttgcaaca tacggattat
      121 caatggaaag cgttgctgta gacattgtgc ctcctagtac aaaaaaaccg cttggtttcc
      181 ctaagcggtt ctaatctata agaactatta caccacaatc tgccccgtct cttttaagta
      241 cttatagata cgccctaaca taatctgcgt tggcgttttg ccaatatcct cttttgttag
      301 cggtgcatca gtaatcgctt tggctgattg agcatcaata tatttgtact cacttgtgat
      361 gattacaaag tttgttacct cgccatcggt atcatcacct gtgccaacaa catacttagc
      421 attgagtgag ccgtcatctt gagttgagta acttgcgata gttgagtaca ttgggtttaa
      481 gattttgtta aaagttgtca taatttttcc ttttgtggat tgttgataaa aaaagaccgc
      541 ttgtagtcat acaaacggct tagtaggaag agtaaattgc tttacataat ataacctttt
      601 atttatactt catttataaa tagtaagtta taaaaggttc aaaaatggca gaccctatag
      661 aatggtctat tatcacgact gactgctttg atgaatggtt cggtcaacaa gatgaaagta
      721 ctcaagaacg tgtcttggct tgtttaatct atctgcggaa attcggttat agtctaggca
      781 gaccttatgc tgatacagtt tatcagtcta aattccctaa tatgaaagag ttacgcattc
      841 aaaacaaagg taagccaatt cgagcatttt atgcttttga ccctttacgc caagcgatag
      901 tactttgtgc tggcgagaaa ggtaaagaca aaagattcta tgaaaaaatg atcgctcttg
      961 ctgatgcgga atttacctcc tatttaaata atttagagaa agaaaatgaa aacgttagat
     1021 caaatgctca atgaactctc tcctgaacgc cgccaaaaag tggaagatat ggctcaagaa
     1081 atgattagag agatacaact ttcaaaacta agagaagagc taaatctctc tcaacaacaa
     1141 ttagctacag ctctcggtat ttcacagcct gctattgcta aacttgaaca gaaagataac
     1201 gatccaagat tatccacgct caaacgctat attgaagcaa tgggcggtaa gttaaaactt
     1261 gccgttgaat tgcctacagg cgatgagcgt gtaattagga tttaattact ttgttaa
//
LOCUS       DANJWC010000251         1312 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.252, whole genome shotgun sequence.
ACCESSION   DANJWC010000251 DANJWC010000000
VERSION     DANJWC010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.252"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..219
                     /gene="lpxB"
                     /locus_tag="PWX02_002380"
     CDS             <1..219
                     /gene="lpxB"
                     /locus_tag="PWX02_002380"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248473.1"
                     /note="catalyzes the formation of lipid A disaccharide
                     from UDP-2,3-diacylglucosamine and
                     2,3-diacylglucosamine-1-phosphate, lipid A disaccharide is
                     a precursor of lipid A that anchors LPS to the OM; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HDL1730340.1"
                     /translation="PLVPELIQEACNPENLAQELSLFLADDEKNQQHKALLKQQFMQL
                     HQSIQCNADEQAAQAVIDLLNGSTSGQI"
     gene            246..1298
                     /gene="sohB"
                     /locus_tag="PWX02_002381"
     CDS             246..1298
                     /gene="sohB"
                     /locus_tag="PWX02_002381"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HDL1730341.1"
                     /translation="MWKEVLLNYGIFLLELLTVFGIIAVVVMLILESKKQNENGSISI
                     TNLSEKYKEQQKSLSGFFLSEEELKHQEKQEKKVAKEKAKAEKKRLKAGEECVESPSR
                     LFVLNFNGDMMAHEVNSLRREIDAVISLANPEKDEVLLKLESPGGVVHGYGLAASQLQ
                     RLRDRNIYLTAAVDKVAASGGYMMACVANKIVSAPFAVIGSVGVVAQVPNIHRLLKKH
                     DIDVDVMTAGEYKRTVTLVGENTEKGKQKFQQELEETHSLFKQFVARRRPQLDIEQVA
                     TGEHWFGQQAIELNLVDEIATSDDLLLNAIKEKEVIELKYKEKKKLAQKLGSQFEQSA
                     ENLLAKILHKSRSTLM"
BASE COUNT          437 a          219 c          308 g          348 t
ORIGIN      
        1 ccattggtac cagaattgat ccaagaagca tgcaacccgg aaaatttagc tcaagagtta
       61 tctctatttt tagctgatga tgagaaaaac caacaacata aagcattgct aaaacagcag
      121 tttatgcagc tacatcaatc aattcaatgt aatgctgatg agcaagctgc ccaagcggtt
      181 atcgatctcc taaacggctc gacaagcggt caaatttagt tagttttttg taaaaaggaa
      241 aatttatgtg gaaagaagta ttacttaatt atggtatttt tttattagag ttattaactg
      301 tatttggcat tattgcagtg gtggtgatgc tgattttgga atcgaaaaag cagaatgaaa
      361 acggctcaat ttcaattacc aatttatctg agaaatataa agagcagcaa aaatctcttt
      421 ccggcttttt tctaagtgaa gaagaattaa aacatcagga aaagcaggag aaaaaagtag
      481 caaaagagaa agctaaagca gaaaaaaaac gcttaaaagc aggcgaagaa tgtgtagaaa
      541 gcccttcccg tttatttgta ttgaatttta atggcgatat gatggctcac gaagtaaatt
      601 ccctacgccg tgaaattgat gcggtaatta gcctcgcgaa tccggaaaaa gatgaagttt
      661 tacttaaact tgaaagtccg ggcggagtgg tgcacggtta tggattggct gcttcgcagt
      721 tacaacgttt gcgtgatcgc aatatttatt taaccgcagc ggtagataaa gtagctgcca
      781 gcggtggtta tatgatggcg tgtgtggcga ataaaattgt ctctgcccca tttgcggtga
      841 ttggctcagt gggggtggtg gctcaggtgc cgaatatcca ccgcttactg aaaaaacacg
      901 atattgatgt ggatgtgatg actgcaggtg aatataaacg cacggtgacg ctggtgggag
      961 aaaataccga aaaagggaaa cagaaattcc agcaagaact ggaagaaacg cactctctgt
     1021 ttaagcaatt tgtggctcga cgccgcccac aactggatat tgagcaggtt gcaacaggcg
     1081 agcattggtt tggtcagcaa gcgattgagc taaatttggt tgatgagatt gcaaccagtg
     1141 atgatctgct cctcaatgca attaaggaaa aagaagtgat cgagctaaaa tataaagaga
     1201 agaaaaaact tgctcagaaa ttgggttctc aattcgagca atcagcagag aacctgctcg
     1261 ctaagatttt acataaaagt cgttcaacgc tgatgtagcg tatatattat ta
//
LOCUS       DANJWC010000252         1298 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.253, whole genome shotgun sequence.
ACCESSION   DANJWC010000252 DANJWC010000000
VERSION     DANJWC010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.253"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            115..381
                     /locus_tag="PWX02_002382"
     CDS             115..381
                     /locus_tag="PWX02_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL1730342.1"
                     /translation="MNKITFTGKADKQLSKIDSRYILAIIEAIESLALFPNVEKDIKR
                     LQGYISRFRMRVGRYRVIFDWLEGEPRIIEIQTIAKRDERTYTH"
     gene            424..822
                     /locus_tag="PWX02_002383"
     CDS             424..822
                     /locus_tag="PWX02_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1730343.1"
                     /translation="MKPYQTIKDTNGNDLFVLVPVAEFEQLTGDNLYDFPYDDETDDD
                     LVEVEYDKDEFDGVTIPFEVVKIKLEQNINNLGAWRIYRNLSQQEVAEKTGLSQSAIS
                     QAERKGNRPQKRTREKLAKIYGCTPEQLAG"
BASE COUNT          463 a          212 c          260 g          363 t
ORIGIN      
        1 acttatattt tattttaaaa cctgatttat acttataaaa agctaataat ttattgatta
       61 gtgaaatata aaaaagtata atggtgttat tgtgaattta taaggaaatc ataaatgaat
      121 aaaattacat tcacagggaa agcagataaa cagctttcaa agattgatag ccgatatata
      181 ctggcaatta ttgaagcgat tgaaagccta gcattatttc ctaacgtaga aaaagacatt
      241 aaaaggctac aaggttatat cagcaggttt agaatgcgtg tggggcggta tagagtgatt
      301 tttgactggc tggagggcga gccaagaatt attgaaattc agactatcgc caaacgagac
      361 gagagaacat atacacacta aagacaaggg gcagaaatgc cccaaacagc acggagaaac
      421 gagatgaaac catatcaaac cattaaagac actaacggca acgatttatt tgtacttgtg
      481 ccggtggcgg aatttgaaca gctaacaggg gacaatttat atgatttccc ctatgatgat
      541 gaaaccgatg atgatttagt tgaagtggaa tatgacaaag atgaatttga tggtgtaact
      601 attccttttg aggtggttaa aatcaagctg gaacagaata taaacaactt aggagcgtgg
      661 cggatttacc gcaatttatc acaacaagaa gtggcagaaa aaacagggct ttcacagtcc
      721 gctatttcac aagcagaacg caaaggcaac cgcccacaaa aacgtacaag ggaaaaatta
      781 gctaaaattt acggttgcac acctgaacag ttagcaggtt aggacctgtc aaaacctcac
      841 attcaaatag ccgttgattg atattaacgg cttttttatt gcttaattct gtaccaatta
      901 cgcaccgatt gcggatcaat acggggcaag ttttcgcaaa atcttaatga aaaatgaccg
      961 cttgaatgcc cttttttata gcgttcagaa acctaccgtt agcctatttt aaatcactgt
     1021 aataaaacac agtttattat ttgtaattta tctgaataat tggcaaaata cagcctataa
     1081 agttcaattc gtaactatac ggatttattc aaattagtgt ggatagtggc ggagtagcta
     1141 ccaaaacgaa aggagaaagc cctttatttc tctttccacg ctctcacagt aaagggcagc
     1201 gttaaagggg cgttatgtat gcttactcaa gaatttttat cactttcgga agctagtgat
     1261 tttgttaaat ctaaaggatt agatctagaa gaatgtga
//
LOCUS       DANJWC010000253         1258 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.254, whole genome shotgun sequence.
ACCESSION   DANJWC010000253 DANJWC010000000
VERSION     DANJWC010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.254"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..659)
                     /locus_tag="PWX02_002384"
     CDS             complement(<1..659)
                     /locus_tag="PWX02_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL1730344.1"
                     /translation="MAVIKEPEQFRETNAFKAFWFHFRQDKLALGSFYLFLLLLALIF
                     VGSLLAPYNYNEQFVGLELMPPSWDDKGQISHFLGTDDLGRDILSRIMYGFYYTLGSA
                     LMITLLIAIIGGMIGIYAGLKKGRSFWLISHLFDTFLFMPILLIAIIIATLMEASLVN
                     AMLAIFLAMLPHFIHKIYQATEKQLKKEYIITLKLDGASHWYLIREVIIPNLTGVAIK
                     AL"
     gene            complement(646..>1258)
                     /locus_tag="PWX02_002385"
     CDS             complement(646..>1258)
                     /locus_tag="PWX02_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL1730345.1"
                     /translation="LAVPVFWLALVLMYYASLNQWEVSAIGEIHSLYPKQMVTGFLLL
                     DIWLSDVPYKLKMMQSALHHLALPTLVLAVPATLEVMHITQARAGYVMKQNYIKIAKT
                     RGWTPFRVWRTHIICNTLPALLPMIARTFILIFAFGMLIENIFSWGGIGRWLINALAI
                     QDYNAISAGVVAIGVFVLLVDMVTGFVRVMLDPSDKKDWYGSY"
BASE COUNT          434 a          267 c          213 g          344 t
ORIGIN      
        1 agtgctttaa ttgccacgcc ggtaagatta ggaataatca cttctcgaat taaataccaa
       61 tggcttgcac cgtctagctt taaggtaatg atgtactctt ttttgagttg tttttcggtt
      121 gcttgataaa ttttatggat aaaatgcggc aacatcgcta agaaaatcgc caacattgca
      181 ttgaccaaac tcgcttccat taaggttgca atgatgatcg caatcaataa aatcggcata
      241 aataaaaagg tatcgaacaa atggctaatc aaccaaaatg aacgtccttt tttcagcccg
      301 gcataaatcc cgatcatacc gccgataatc gcaattaata aggtaatcat cagtgcggaa
      361 ccaagcgtgt agtaaaaacc atacataata cggcttaaaa tatcccgtcc taaatcgtca
      421 gtgcctaaaa aatggctgat ttgacctttg tcgtcccaag agggaggcat taattctaaa
      481 ccaacaaatt gttcattata attgtaagga gcaagcaaac taccaacaaa aatgagagcc
      541 agtaatagca aaaaaagata aaaactgcca agtgcaagtt tatcttgtcg gaaatgaaac
      601 caaaatgcct taaatgcgtt ggtttctcta aattgctcgg gttctttaat aactgccata
      661 ccaatccttt ttatctgatg ggtctaacat cacccgcaca aagcctgtaa ccatatctac
      721 taataacaca aacaccccga ttgcaactac tcccgctgaa atcgcattat agtcttgaat
      781 agcaagggca tttatcagcc aacgtccaat tcctccccag ctaaatatgt tctcaattaa
      841 cataccgaag gcaaaaatta aaataaaggt acgggcaatc ataggtaata atgcaggtaa
      901 agtattacaa ataatatgcg ttctccaaac tctaaacggt gtccagccac gcgttttcgc
      961 tattttaatg taattttgtt tcattacata acctgctcta gcttgagtaa tatgcataac
     1021 ttctaatgta gctggcacag ccaagactaa tgtcggcaaa gctaagtgat gtaaagcact
     1081 ctgcatcatt tttaatttat aaggtacatc agataaccaa atatctaata gcaaaaagcc
     1141 ggttaccatc tgtttcggat aaagcgaatg gatttctcca attgctgaca cttcccactg
     1201 attgagagaa gcgtaataca ttagcactaa tgccagccaa aataccggca cggctaaa
//
LOCUS       DANJWC010000254         1243 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.255, whole genome shotgun sequence.
ACCESSION   DANJWC010000254 DANJWC010000000
VERSION     DANJWC010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.255"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..>1243
                     /locus_tag="PWX02_002386"
     CDS             <1..>1243
                     /locus_tag="PWX02_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL1730346.1"
                     /translation="KYLNHPSFNFPLSLNEVSLIKSGTTPTDRDDNLKEGVVLLKTND
                     IRNNLLNKYSSVDYFISEDINEKMISSQLKEGDVLVNIVGATLEVVGRVAYVSSTFPK
                     ANITQAMSFVRLKSKYNKELLPTYLFAFLQSSYGKIQINRNARPTGQYNLNNEELGAI
                     KVPLIDLETQKQVDKIIKQSNDFVQKSTQSYQEAETLLLENLGLRAFQADSNPVNVKS
                     LKESFLQTGRLDAEYYQTKYEQYWNLIQSQDYVFIRDEYLHITQKPDWTKPMYQYIEI
                     GDVNIGDGSYQTNWIETQELPANAKTQAQTGDILISTVRPYRGAVTIIGENDQDLVVS
                     GAFTVLRRKENSVFNNEVLKVLLRSELYKDWLLQFNVGTSYPVIKDNDILNLPIPKIS
                     GEIQEKIAEYIRQSNDLRQQAQ"
BASE COUNT          466 a          178 c          219 g          380 t
ORIGIN      
        1 aaaatactta aaccatccga gttttaattt tccattatcg ctgaatgagg tttctctgat
       61 taaaagtggt acaacaccaa cagatcgaga tgataactta aaagaggggg ttgtcttatt
      121 aaaaaccaat gatattagga ataatctatt aaataagtac tcttcggtag attattttat
      181 tagcgaagat atcaatgaga aaatgatttc atcacaactg aaagaagggg atgttttagt
      241 aaatatcgtt ggtgcaactt tggaggttgt tggtcgggta gcttatgtta gtagcacttt
      301 tccaaaagca aatattacac aagcaatgtc ttttgtaaga ttaaaatcaa aatataataa
      361 agaattatta ccaacgtatt tatttgcttt tttacaaagt tcttatggaa aaatccaaat
      421 taaccggaat gcacgtccaa caggacaata caatctgaat aatgaggagc ttggtgcgat
      481 taaagtcccc ttaattgatc tagaaacaca aaagcaggta gataaaatca ttaagcaaag
      541 taatgatttt gtacaaaaat caacacaaag ttaccaagaa gctgaaactt tattattaga
      601 aaatctcggt ttgagagctt ttcaagctga tagcaatcct gtaaatgtta aatccttaaa
      661 agagagtttc ttacaaacag gacgattaga tgcagagtat tatcaaacta aatatgagca
      721 atactggaat ttaattcaat cgcaagatta tgtttttatt cgtgatgaat atttacatat
      781 cacccaaaaa cctgattgga caaaaccgat gtatcaatat attgaaattg gtgatgttaa
      841 tattggtgat ggttcatatc aaacgaattg gatagagaca caagaattac ctgctaatgc
      901 taaaactcaa gcccaaacag gtgatatttt aatttcaacc gttcgccctt atcgtggtgc
      961 agtaacaatt atcggtgaaa atgatcaaga tttagttgtt agtggtgcgt ttacagtatt
     1021 acgcagaaag gaaaactctg tttttaataa tgaagtttta aaggtgctat tacggtcaga
     1081 actatataaa gattggttac tacaatttaa tgtaggtacg agttatcctg taattaaaga
     1141 taacgatatt ttgaatttac caattcctaa aataagtgga gaaatccaag aaaaaattgc
     1201 tgaatatatc cgccaaagca acgatttaag gcagcaagca caa
//
LOCUS       DANJWC010000255         1213 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.256, whole genome shotgun sequence.
ACCESSION   DANJWC010000255 DANJWC010000000
VERSION     DANJWC010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.256"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..275)
                     /gene="raiA"
                     /locus_tag="PWX02_002387"
     CDS             complement(<1..275)
                     /gene="raiA"
                     /locus_tag="PWX02_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDL1730347.1"
                     /translation="MSLNISSKQMDITPAIRTHIEERLAKLEKYHTQLINPHFMLIKQ
                     PNSFQVEATIGTPLGDLVAKAEHEDLYTAIFDVKKKLEGRLIKLKEK"
     gene            455..>1213
                     /gene="lpxL"
                     /locus_tag="PWX02_002388"
     CDS             455..>1213
                     /gene="lpxL"
                     /locus_tag="PWX02_002388"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferase"
                     /protein_id="HDL1730348.1"
                     /translation="MSEKKQLPPFQTQFLHPKYWGLWLGLGVFKLILCLPYPILVKIG
                     LGLGKLFTKLGFGKRRMRIARRNLELCFPHYSAEKVEELVEENMKSVGMAIIETGMAW
                     FWSDKRILKWSKIEGLEHLTQRDQERGILLVGVHFLTLELGARIIGLHHQGVGVYRPN
                     DNPLLDWIQFRGRVRSNKAMLDRKDVRGMIRALKNGEVIWYAPDHDYGRRNSVFVPFF
                     AVEETCTTAGSYMLLRSAPKSLVIPFSPIRNLDYS"
BASE COUNT          353 a          184 c          255 g          421 t
ORIGIN      
        1 tttttctctt ttagtttaat taaccgacct tctaatttct tcttaacatc aaaaattgca
       61 gtatataaat cttcatgttc agctttagcg actaaatcac ctaaaggggt gccaattgtt
      121 gcttcaactt ggaatgagtt aggttgtttt atcagcataa aatgtgggtt tatcaattgg
      181 gtgtggtatt tttctaactt agctaaacgc tcctcaatat gagtacggat tgcaggggta
      241 atgtccattt gtttgcttga aatatttaat gacataacat tttcctcttg tttagttaag
      301 gcttttgccg ttgatattct aataaccaaa atatgctcat cactccaaaa attcaagtga
      361 aaaatgaaat aagtttccaa aaatttgatc tacttaacac ttttagcagt ttttagctat
      421 aatgtaatca taatctagta tgtgagaata attaatgagt gagaaaaaac agttacctcc
      481 attccagaca caatttttac accctaaata ttggggattg tggctcggtt taggtgtttt
      541 taagttaatt ttatgtttgc cttatcctat tttagtaaaa attggtctgg gactaggtaa
      601 attgtttact aaattgggtt ttggtaaacg tcgtatgcgt attgctcgcc gtaatttaga
      661 gctctgtttt ccccattact ctgctgagaa agtagaggaa ttagtcgaag aaaatatgaa
      721 atcggttgga atggcgatta ttgaaaccgg tatggcttgg ttttggtcgg ataaacgtat
      781 tcttaaatgg tctaaaatcg aagggctaga gcatttaaca cagcgtgatc aggaaagggg
      841 tattttactt gtaggggtgc attttctgac tttagaactc ggtgcaagaa ttattgggct
      901 acatcatcaa ggagtagggg tttatcgacc gaatgataat ccattattag attggataca
      961 gtttcgagga cgtgttcgct caaataaagc gatgttagat cgtaaagatg tgcgtggaat
     1021 gattcgagct ttaaaaaatg gcgaggtgat atggtacgcg cctgatcacg attatggaag
     1081 acgaaatagc gtttttgtac cattttttgc agtggaggaa acttgtacta ctgcgggtag
     1141 ttatatgtta cttcgttctg ccccaaagag tttagtgatc ccgtttagcc cgattcgaaa
     1201 tttggactat tca
//
LOCUS       DANJWC010000256         1194 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.257, whole genome shotgun sequence.
ACCESSION   DANJWC010000256 DANJWC010000000
VERSION     DANJWC010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.257"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..628)
                     /locus_tag="PWX02_002389"
     CDS             complement(<1..628)
                     /locus_tag="PWX02_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HDL1730349.1"
                     /translation="MKKYLISLEKDVKRRELFFSQPNTEDFEVFSAINTMSSDWEALN
                     NHFDLALFEQRYGRKTTKGEVGCTLSHLAVYQKIAEDRTIAEDDYCLVCEDDALFADD
                     FQKHLDALLAQNLKVDIILVGQSKIPTFNDSELELNYPSTFGFLQKNIPNSQYKYAYS
                     YLNYFAGTVAYLIKKSTACQFLAQCAGKNPYWLADDFILFEKNFKFDVL"
     gene            complement(630..>1194)
                     /locus_tag="PWX02_002390"
     CDS             complement(630..>1194)
                     /locus_tag="PWX02_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CMP-N-acetylneuraminate-beta-galactosamide-
                     alpha-2, 3-sialyltransferase"
                     /protein_id="HDL1730350.1"
                     /translation="AENIGLNRKLINFFLGNKYSLEKLKQRSKTHYTIYKNFPNIIEN
                     TVYIDLINQVESVGTAPSNDDITHILLGQPIFERDDEKNIALAEKVIQQFGIDLYLPH
                     PREKYELENVEYINTNLIFEDYIFQEFSHKKCRVYTYFSSAVINILNKSNHIEVVALR
                     VNVDNPAYIESYELLEKLGVQIVDIRE"
BASE COUNT          395 a          209 c          161 g          429 t
ORIGIN      
        1 ttaacacatc aaatttgaaa ttcttttcaa ataaaataaa atcatcggca agccagtatg
       61 gattttttcc ggcacattgt gctaaaaact gacaagcggt cgatttttta attaaatatg
      121 caaccgtacc agcaaaataa ttcaggtaag aataagcata tttatattgg ctattcggaa
      181 tatttttttg caaaaagcca aaggttgatg ggtaattaag ttctaattcg ctatcgttaa
      241 aagttggaat tttcgactgc ccaactaaaa taatatcgac ttttaaattt tgtgctaata
      301 aagcatcaag atgtttttgg aaatcatcgg caaataaggc atcatcttca cagactaggc
      361 agtaatcatc ttccgcaata gttctatctt cggcaatttt ttggtaaacg gctaaatggc
      421 ttaaagtaca acctacttcg cccttagtgg ttttacgccc atatcgctgt tcaaacagag
      481 ccaaatcaaa atggttattt aatgcctccc aatcactact cattgtatta atggcactga
      541 acacctcaaa atcttccgta ttaggctggc taaaaaatag ctctcgtctt tttacatctt
      601 tttctaatga aattaaatat ttcttcattt tattctctaa tatcaacaat ctgaacgcct
      661 aatttttcaa gcaattcata gctctcaata taagcaggat tatctacatt cactcttaat
      721 gctaccactt caatatggtt acttttattt aaaatattga tcaccgcact actaaaataa
      781 gtatagactc tacatttttt atggctaaat tcttggaaaa tataatcttc aaaaattaga
      841 ttagtattaa tgtattctac attttctaat tcatattttt ctcttggatg cggtaaatat
      901 agatcaatgc caaattgttg gataactttt tccgccaatg ctatattttt ttcatcatct
      961 cgttcaaaaa taggctgtcc taataaaata tgggtaatat catcgttaga tggtgctgta
     1021 cccactgatt caacctgatt aatgagatca atataaaccg tattttcaat aatattcgga
     1081 aaatttttgt aaattgtata atgagtttta gagcgttgtt tcagcttttc taggctatat
     1141 ttattaccta aaaaaaagtt aatcaatttt cgatttaacc caatattttc agct
//
LOCUS       DANJWC010000257         1171 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.258, whole genome shotgun sequence.
ACCESSION   DANJWC010000257 DANJWC010000000
VERSION     DANJWC010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.258"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            23..1027
                     /locus_tag="PWX02_002391"
     CDS             23..1027
                     /locus_tag="PWX02_002391"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005651072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDL1730351.1"
                     /translation="MAFNLKNRHLLSLVNHTEREIRFLLDLARDLKRVKYAGTEQQKL
                     KGKNIALIFEKTSTRTRCAFEVAAYDQGAHVTYIDPNSSQIGHKESMKDTARVLGRMY
                     DAIQYRGFKQSVVQELADYAGVPVFNGLTDEFHPTQMLADVLTMIENCEKPLSQISYV
                     YIGDARNNMGNSLLLIGAKLGMDVRICAPKALLPEDAFVKMCEKFAAESGARMTVTED
                     IETAIKGVDFVHTDVWVSMGEPLESWTERIDLLMPYRVTPELMQATDNPKVKFMHCLP
                     AFHNSETKVGKEIAEKYPALANGIEVTEEVFESPMNVAFEQAENRMHTIKAVMVASLA
                     "
BASE COUNT          380 a          215 c          277 g          299 t
ORIGIN      
        1 aatgtttaca gggagaaaca aaatggcatt taacttaaaa aacagacatt tactcagttt
       61 agtaaaccac actgagcgtg aaattcgctt tttgttggat ttagcaagag atttaaaacg
      121 ggtgaaatat gctggcactg agcagcaaaa attaaaaggc aaaaatattg ccttaatttt
      181 tgagaaaacc tcgacccgta ctcgttgtgc ctttgaagta gcagcttacg atcaaggggc
      241 tcacgttacc tatattgacc cgaactcctc gcaaatcggg cataaagaat caatgaaaga
      301 taccgcccga gtgctaggca gaatgtatga tgcgattcaa tatcgcggct ttaaacaatc
      361 ggtagtacaa gagcttgccg attatgccgg cgtgccggtg tttaacggct taaccgatga
      421 attccaccca actcaaatgt tggctgacgt gctaacgatg atcgaaaatt gcgaaaaacc
      481 gcttagccaa atcagttatg tctatatcgg cgatgctcgc aataatatgg gtaattcact
      541 cctgttgatt ggggcgaaat tggggatgga cgtgcgaatt tgtgccccga aagccttatt
      601 accggaagat gcattcgtga aaatgtgtga aaaatttgca gctgaatcgg gggcaagaat
      661 gacggtaacc gaagatattg agactgcaat taaaggtgtc gattttgtgc atactgatgt
      721 ttgggtttca atgggtgaac cgctagagag ctggactgaa cgtatagatt tattaatgcc
      781 ttaccgagta acgcctgaat taatgcaagc aaccgacaac ccgaaagtga aatttatgca
      841 ctgcttaccg gcgttccaca acagtgaaac taaagtcggt aaagagatcg ccgaaaaata
      901 tccggcactg gcaaacggca ttgaagtaac cgaagaggtg tttgaatcgc caatgaatgt
      961 ggcattcgag caagcagaaa atcgaatgca cactattaaa gcggtaatgg tagcaagttt
     1021 ggcataatag ctaagtctaa aaagcggatt atcttgggtg gtaatctgct ttttagctta
     1081 aggctagatc atcgtagctg aagaagattt gtggaaaaca ctaacccggg gagtaaatta
     1141 aaaattcaaa taaaaatctt gggtaagtct t
//
LOCUS       DANJWC010000258         1171 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.259, whole genome shotgun sequence.
ACCESSION   DANJWC010000258 DANJWC010000000
VERSION     DANJWC010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.259"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..1034)
                     /locus_tag="PWX02_002392"
     CDS             complement(<1..1034)
                     /locus_tag="PWX02_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL1730352.1"
                     /translation="MLNTQFRTHFPFFEKSDGWTYLDSAATTLKPDVLIEATSEFYAS
                     AGSVHRSQYDLPQTEQYELARDLVAKRFNVETREAVIWTSGTTQSINTVAYGLEHQIA
                     AGDEIVISVAEHHANFIPWQQLAQRTRAKLVVLPLNDKYEIELETLKNALSEKTKIVA
                     FNLVSNVTGVRQPAEQIIPLIRQSCSAKIVLDIAQAVCSEQVDVQKLDADFYAFSAHK
                     MYGPNGVGVLTGKLQSLEQIQPLVFGGKMLKNITESELTVAELPYRLEAGTPNIAGII
                     GFGQVLAWLEKWDFVDLNHQLYDLAEYTCKRLNSYQNLQIIGQQNTPTISFVVKNQHP
                     ADIAAFLTQS"
BASE COUNT          338 a          239 c          241 g          353 t
ORIGIN      
        1 ttactttggg ttaagaaagc ggcaatatcg gcagggtgct gatttttcac cacaaagcta
       61 atagtcggtg tattttgttg cccgataatt tgcaaatttt gataagaatt taaccgcttg
      121 caggtgtatt cagccaagtc ataaagctga tgattaagat cgacaaaatc ccatttttcg
      181 agccaagcta gcacttgccc aaagccgata atgccggcga tattcggcgt gccagcttcc
      241 aagcgataag gtaactctgc cacagttaat tcactttccg taatgttttt cagcattttg
      301 ccgccaaata caagcggttg aatttgctcc aaactttgca atttgccggt aagcacgccc
      361 acaccgtttg gaccatacat tttatgggcg gaaaaagcgt agaaatctgc atctaatttc
      421 tgaacgtcca cttgctctga gcaaactgct tgagcaatat ctaacacaat tttagcagag
      481 caagattggc ggataagcgg gattatttgt tccgctggct ggcgaactcc tgttacgtta
      541 gaaactaaat taaaggcaac aattttggtt ttttccgata acgcattttt aagggtttca
      601 agctcaattt catacttatc attaagtggc aacaccacga gtttcgccct tgtacgctgt
      661 gccaactgct gccaaggaat aaaattagcg tgatgttccg ccactgaaat tacaatttca
      721 tcgcctgctg caatttgatg ctccaagcca taagccacgg tgttaatact ttgggttgta
      781 ccgcttgtcc aaatcaccgc ttcacgagtt tccacattga aacgttttgc gactaaatct
      841 cgagctaatt catattgttc ggtttgtggc aaatcgtact gactgcgatg caccgacccc
      901 gcagaagcgt aaaactcact tgttgcttca atgagtacat caggtttcag cgttgtagcg
      961 gcagaatcta aatacgtcca tccgtcagat ttttcaaaaa aagggaaatg ggtgcgaaat
     1021 tgggtgttaa gcataagtta attcctaaga gttgtggaaa attgagttac caagaaaata
     1081 cctattataa aggaatgttt tgaggtaaga agcatttcaa gaaataaata gatttatttt
     1141 gttatctttt tgttaaattc gtgttgacta a
//
LOCUS       DANJWC010000259         1165 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.260, whole genome shotgun sequence.
ACCESSION   DANJWC010000259 DANJWC010000000
VERSION     DANJWC010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.260"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            714..962
                     /locus_tag="PWX02_002393"
     CDS             714..962
                     /locus_tag="PWX02_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730353.1"
                     /translation="MISGEKMTWESLGASAIGGAIGGGIGATMKAGAVTASTLSALGG
                     GMGEGLYKSTSQGIKNSIGIKASCNQSGSDYGDGCDYQ"
     gene            974..>1165
                     /locus_tag="PWX02_002394"
     CDS             974..>1165
                     /locus_tag="PWX02_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730354.1"
                     /translation="MKIQFPISYQEFRENYFEKKPLLMKGAISQKDLLSWKSINEILP
                     RCDLISEDAIKVMHKGKRAH"
BASE COUNT          397 a          123 c          249 g          396 t
ORIGIN      
        1 tatcaatgaa gtattacctc gttgtgattt actttctgaa gatgcaatta aagtaatgta
       61 taaagtaagt tgaaatactt taatggagaa agtatggtgg atttttctat tttgatatta
      121 gttatagtat tgcctgttat ttttgtgaaa gttatagatg tactgttgct tagtaagtta
      181 gatatgagta aggaaaaaaa tgacaaatgc caaataataa tatatttatt tataataatt
      241 ttacttgggg catataagag ctattttatt caaaaataaa taagaggaga gaaaatgaaa
      301 gatcttagta ttaaggattg tgaaataata tatggaggca tagccccttt gtggtgggat
      361 ggtactggca ttacatcata ctgggaacta aattatacca ccctaccact agtattttag
      421 tttatacggc tagataaata tgatttttga aaaaatatta cttatattga ttatcccatt
      481 catattatca agaattgtgt ctattcttct tgtaaagaag attgtaaaaa ataaaaatgt
      541 acaagatgtt attgaggcta tagttctaat agtatttgtt gtaattataa acatcataga
      601 ggaatatctc ttatgaaaga gttaaaaatt gaggattgcc aatttgtttc ggcaggcttg
      661 cacttgttat tgtaggagct gttgctggag ctgcatatta tgttgggaat agtatgattt
      721 ctggggaaaa aatgacttgg gagagtttgg gagcatcagc tataggtggg gctattggtg
      781 gcggtattgg agcaacaatg aaagctggtg cggtgacagc gtcaacctta tctgctttag
      841 gtggtggtat gggggagggc ttgtataaaa gtacttctca aggtatcaaa aatagtattg
      901 gtataaaggc gagttgcaat caatcaggtt cggattatgg tgatggatgt gattatcaat
      961 aagagagtat attatgaaaa ttcaatttcc aatttcttat caagaatttc gtgaaaacta
     1021 ttttgagaaa aaaccacttt taatgaaagg agcaatatct cagaaagatt tactttcttg
     1081 gaagtcaata aatgaaatat tacctcgctg tgatttaatt tctgaggatg caattaaggt
     1141 aatgcataaa gggaaaaggg ctcat
//
LOCUS       DANJWC010000260         1136 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.261, whole genome shotgun sequence.
ACCESSION   DANJWC010000260 DANJWC010000000
VERSION     DANJWC010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.261"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            225..941
                     /locus_tag="PWX02_002395"
     CDS             225..941
                     /locus_tag="PWX02_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HDL1730355.1"
                     /translation="MYLMYVDESGSTTDPTQKYFVLSGIAVFERNTHWIEKKMNVIAS
                     QFCQYSEGSDPYFYELHGSPMRSGKSEWRGIPMSERINAIQDCLKLVQSNNIRIFAAV
                     IEQGYSSGQDTITECFEQISSRFDMYLSRLHAQGNTQRGISIFDKSSTEKSIQALART
                     FKNSGHTYGKLRNFSEVPLFLDSKSSRLIQLADLVSYAIFRHYEHNDSSFYDIIRNNF
                     DYHNGRIHGLYLRNKEDIIS"
     gene            complement(984..>1136)
                     /locus_tag="PWX02_002396"
     CDS             complement(984..>1136)
                     /locus_tag="PWX02_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL1730356.1"
                     /translation="RFRHTVATQLMKKPENVYVVQKLLGHKDISVTLGYIEHDVEMLR
                     ETVNLL"
BASE COUNT          360 a          187 c          200 g          389 t
ORIGIN      
        1 gatccaggtc ttacacattc tttcaagttt gtaaagcctg aaaattattt tttatacttg
       61 atttttgtca aaaagtctct atactatatg gcagggtcag ttttcgctgt ttaacggtgt
      121 ttaatgagat tttcattact ggctctcctt aatagaaatt tatggaagag ctatctgata
      181 gagtaatcta gcgatagctc tttttcattg aacaaagagg ctatatgtat cttatgtatg
      241 ttgatgagtc tggttctacg acagatccta ctcaaaaata ttttgtatta tcaggtatcg
      301 ctgtatttga acgaaatact cattggattg agaaaaaaat gaatgtgata gcctctcaat
      361 tctgtcaata ttctgaaggg agtgatccat atttctatga gttacatggt tcaccaatga
      421 gatctggaaa gagtgaatgg agaggcattc caatgagtga gcgtattaat gccattcaag
      481 attgcttgaa attagttcaa agtaataata ttcgtatatt tgcagcagtt atagagcaag
      541 ggtattctag tggtcaggat actattacag aatgttttga gcaaatatca tctcgatttg
      601 atatgtatct gagccgttta catgcccaag gtaataccca aagaggcatt tctatctttg
      661 ataaatcatc gactgagaag tcaatccaag cacttgcaag aacattcaaa aatagtgggc
      721 atacttatgg gaaacttaga aatttctcag aagttccatt atttcttgac tcaaagtcat
      781 cgagattaat tcagctagcg gatctagttt cctatgcgat atttagacac tatgaacaca
      841 atgatagctc attttacgat attataagaa ataactttga ttatcataac gggcgtattc
      901 atggtttata tttacggaac aaagaagata ttatttcata aaaattcgcc ccaaaaggga
      961 gaagtatctc ccttttgtat tttttataac aaattaaccg tttctctcaa catttccaca
     1021 tcgtgttcaa tatatcctag tgtcacactg atatctttat gccctaacag tttctgaacc
     1081 acataaacat tttcgggctt cttcattaat tgagtagcaa ctgtatgtcg aaatct
//
LOCUS       DANJWC010000261         1088 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.262, whole genome shotgun sequence.
ACCESSION   DANJWC010000261 DANJWC010000000
VERSION     DANJWC010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.262"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            178..360
                     /locus_tag="PWX02_002397"
     CDS             178..360
                     /locus_tag="PWX02_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDL1730357.1"
                     /translation="MKIMQLTSNQYINDIKHILLSARQKAYQAVNSAMVEAYWKIGER
                     IVQEEKNMVKDQNEKV"
     gene            347..>1088
                     /locus_tag="PWX02_002398"
     CDS             347..>1088
                     /locus_tag="PWX02_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL1730358.1"
                     /translation="MKKYNIVFSADKNYLPYLEIALKSLLAHHNHLSIFILSTGDISH
                     HWIETLRPYLENRYSTLRLGYLDSNNLANFNSNGYITNSTYLRYYIEELFEHSDSPYW
                     IYLDCDLVVNGNITDPFTNLDFSFGSIAAIADPYVNKLKNHPYIYKNYFNAGVFYLNA
                     KHFSKQPFIQLTERFKENLPFGDQDILNYCFQYNWIELDRKYNYQTEHLLDDIAQQKI
                     SPNYIPHIIHFTGGIKPLEGKFNSSYVEN"
BASE COUNT          395 a          175 c          173 g          345 t
ORIGIN      
        1 ctttgtgcca agccatttta ttagcgaaga taaattgata tgcgattata acccaacgga
       61 acttgttgca gcacaaggcg agagagtaga attccttgaa ctacattacc aatgggcaat
      121 tgtcaaaaga gaaaacgaaa ttggctggct accttgtgaa atcttaaggg taataggatg
      181 aaaattatgc aacttacgtc aaatcagtat atcaacgaca ttaaacatat tcttttatct
      241 gctcgccaaa aggcgtatca agccgtgaat tcggcaatgg tcgaagccta ttggaaaatc
      301 ggtgaacgaa ttgtgcaaga agagaaaaat atggtcaaag atcaaaatga aaaagtataa
      361 tattgtattt tcagcagata aaaactacct gccatattta gagattgcct taaaatcact
      421 attagctcat cataatcatt tatctatttt tatattgagt actggcgata tttctcacca
      481 ctggatagaa acattacgcc cctatttaga aaatagatat tccacattaa gacttggcta
      541 tttagattca aataatttag caaattttaa ctccaacggc tatattacta attcaactta
      601 cctacgatat tatattgaag aactatttga acacagcgat agcccatatt ggatttattt
      661 agattgcgat cttgtggtta atggaaatat tacggatcca tttacaaacc tagatttttc
      721 ttttggaagt attgctgcaa ttgctgatcc ttatgtaaac aaactaaaaa atcatcccta
      781 tatttacaaa aactatttca atgctggtgt attttatcta aatgcaaagc attttagtaa
      841 gcaaccattt atacagctta ctgagagatt taaagaaaat ttaccatttg gggatcaaga
      901 tattcttaat tactgctttc aatataattg gattgagtta gatcgaaaat ataattatca
      961 gacagaacat ttattagatg atattgccca gcaaaaaatt tcgccaaatt atattccaca
     1021 tataattcat tttactggag gaataaagcc attagaaggt aaatttaatt caagctacgt
     1081 tgaaaacg
//
LOCUS       DANJWC010000262         1085 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.263, whole genome shotgun sequence.
ACCESSION   DANJWC010000262 DANJWC010000000
VERSION     DANJWC010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.263"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..987)
                     /gene="rfbB"
                     /locus_tag="PWX02_002399"
     CDS             complement(<1..987)
                     /gene="rfbB"
                     /locus_tag="PWX02_002399"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDL1730359.1"
                     /translation="MSLNVLITGGAGFIGSALIRFLAKQTDHHIINVDKLTYAANLAS
                     IKTVQNSERYYFEQVDICASGQISEIFCKYQPDAVMHLAAESHVDRSIKSAGEFIQTN
                     IVGTYSLLEVTRQYWEHLPEPRKSAFRFIHISTDEVYGDLPLTAEPSAENLPYAPSNP
                     YSASKASADHLVRAWYRTYGLPAIITNCSNNFGPYQYPEKLIPLMISNALQGKALPIY
                     GDGLQIRDWIFVEDHIRALYDVLIKGKLGASYNIGSHQEKSNIEVVYSICELLEELVP
                     NKPKNVKKYVDLIRYVQDRPGHDVRYALDSTKIQKELGWKPQETFESGLRKTV"
BASE COUNT          369 a          211 c          182 g          323 t
ORIGIN      
        1 tactgtttta cgaagaccac tttcaaatgt ctcctgaggt ttccagccta actctttttg
       61 aattttagtt gaatctaatg cgtagcggac atcatgcccg ggccggtctt gaacatagcg
      121 aattagatca acatattttt taacattctt aggcttattt ggcactaact cctctaacag
      181 ctcacaaatt gaataaacca cctcaatatt gcttttttct tgatgggagc caatattata
      241 acttgcgcct aatttacctt taattaaaac atcgtataaa gcacgaatgt gatcttcgac
      301 gaaaatccaa tcccgaattt gcaaaccatc accataaata ggcaaagcct ttccttgcaa
      361 tgcatttgaa atcattaatg ggatcaattt ttcaggatat tgataaggac caaaattatt
      421 ggagcaattt gtaataatcg ctggtaaacc ataagttcta taccaagcac gtactaaatg
      481 atcagccgat gctttggaag cagaataagg gttacttggg gcataaggta aattttcagc
      541 agatggttca gctgttaatg gcaaatcacc atacacttca tctgttgaaa tgtggataaa
      601 acgaaacgct gattttcgag gttcgggcaa atgctcccaa tattgtcgag tgacttctaa
      661 cagagaataa gttccaacaa tattagtttg aataaactca ccagcactct tgattgagcg
      721 atcaacatgg ctttcagcag ccaaatgcat caccgcatca ggctgatatt tgcaaaaaat
      781 ctcggaaatt tgaccacttg cacaaatatc aacttgctca aaataataac gctcagaatt
      841 ctgtacggtt ttgattgagg ctaaatttgc agcataagtg agcttatcta cattgattat
      901 atgatggtct gtttgcttag ctaaaaaacg aattaacgcg gagccgataa atccggctcc
      961 acctgtaatt aatacattta aactcatcat ataactaata ggctaaggaa ataaatatct
     1021 taaaaaggaa ttttgcaaaa aaatatcgca atcacacgct tacaatatat aaacatagat
     1081 aataa
//
LOCUS       DANJWC010000263         1065 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.264, whole genome shotgun sequence.
ACCESSION   DANJWC010000263 DANJWC010000000
VERSION     DANJWC010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.264"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..138
                     /locus_tag="PWX02_002400"
     CDS             <1..138
                     /locus_tag="PWX02_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250560.1"
                     /note="Acylates the intermediate (KDO)2-lipid IVA to form
                     (KDO)2-(lauroyl)-lipid IVA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HDL1730360.1"
                     /translation="QNEVETAILMNRVVEREIMAALTQYMWLHRRFKTRPNEGDQSLY
                     D"
     gene            234..>1065
                     /gene="dapA"
                     /locus_tag="PWX02_002401"
     CDS             234..>1065
                     /gene="dapA"
                     /locus_tag="PWX02_002401"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDL1730361.1"
                     /translation="MATPLFHGSIVALVTPMTHGEVDYQDLKKLVEYHIQAGSHAIVS
                     VGTTGESTTLSIDENVKVIKKTVEFADGRIPIIAGTGSNATSEAITMTKLLANSGVAG
                     CLSVVPYYNKPTQEGMYLHYKAIAESTDLPQILYNVPGRTGSDLKPETIGRLAKIENI
                     VAVKEATGDLSRLPLIKQLAGEDFIFLSGDDATGFESMKLGGQGVISVTNNVAAADMA
                     KMCELALAGQFTEAEEINNRLMALHHDLFVESNPIPVKWAAYKLGLISEPNLRLPLTT
                     L"
BASE COUNT          340 a          171 c          232 g          322 t
ORIGIN      
        1 caaaatgaag ttgaaacggc tattttaatg aatcgggtgg tagaacgtga aattatggct
       61 gcgctaactc aatatatgtg gctacatcgc cgttttaaaa ctcgcccaaa tgaaggtgat
      121 caaagcctat atgattaatt ttaaataatt tcaaaaaagt gcttgcatat ttaagctgaa
      181 ttttgcaatc tagcaatgtt atcaagcata ataaaaaaat acaggagatg attatggcga
      241 cccctttatt tcacggtagt atcgtagcat tagttacacc aatgactcat ggtgaggtag
      301 attatcagga tttaaaaaaa ttagttgagt atcatattca agcaggttct cacgctattg
      361 tatctgttgg tacaacaggt gaatctacca cattaagcat tgatgaaaat gttaaggtga
      421 ttaagaaaac ggttgaattt gctgatggac gtattccaat tattgcagga actggttcta
      481 atgcgaccag tgaagctatc actatgacta aattattagc aaacagtggt gtagctggtt
      541 gtttaagtgt agtgccttat tacaacaagc caacgcaaga ggggatgtac ttacactaca
      601 aagcgattgc agaaagtaca gatttaccac aaattctgta taatgtgccg gggcgaacag
      661 ggagtgattt aaaacctgaa acaatcggtc gcttagcgaa aattgagaat attgttgctg
      721 tgaaagaagc aacgggtgat ttaagccgct tacctttaat taagcaatta gccggtgaag
      781 attttatttt cttaagtggt gatgatgcta ccggttttga gtctatgaaa ttaggtggtc
      841 aaggggtgat ctcggtcacc aataatgttg ctgctgctga tatggcaaaa atgtgtgagt
      901 tagctcttgc cggtcaattt actgaagcag aagaaattaa taaccgctta atggcgttac
      961 accatgactt atttgtcgaa tctaacccga ttccggtgaa atgggctgct tataaattag
     1021 gtttaattag tgagcctaat ttacgtttac cgcttaccac actgt
//
LOCUS       DANJWC010000264         1065 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.265, whole genome shotgun sequence.
ACCESSION   DANJWC010000264 DANJWC010000000
VERSION     DANJWC010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.265"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..478
                     /locus_tag="PWX02_002402"
     CDS             <1..478
                     /locus_tag="PWX02_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL1730362.1"
                     /translation="EVGDWSSGATPNRHNPEYYNGTIPWLKTGDLNNGIISSIPEFIT
                     DKALQECSVRLNPVGSVLIAMYGATIGKLGILKIAATTNQACCACIPFNGIYNKFLFY
                     YLMSQKAEFQKKSEGSGQPNISKEKIINYLFPLPPIHEQHRIVQKIEQLFAEIEKL"
     gene            581..>1065
                     /locus_tag="PWX02_002403"
     CDS             581..>1065
                     /locus_tag="PWX02_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDL1730363.1"
                     /translation="MIHLFEKHLNQSNLAENTQSAYLFAVKQYHKKFDQLSKINLQRY
                     KVFLIENYKPQTVNLRLRAINYYLEFIKKEKWKLSFVKVQQKPFLENVISEADYTYFK
                     KCLKKDNELYWYFVIRFMAATGSRVSELIQIKCEHIKIGYLDLYSKGGKLRRIYIPKS
                     L"
BASE COUNT          397 a          164 c          175 g          329 t
ORIGIN      
        1 ggaagtaggt gattggagtt caggtgcaac tccaaaccga cacaatcctg aatactataa
       61 tggtacgatc ccgtggctaa aaacaggaga cttaaacaac ggaataattt catctatacc
      121 tgaatttatt acagataaag cattgcagga atgttctgta agattaaatc ctgttggttc
      181 agttttgata gcaatgtatg gagcgacaat tggtaagctt gggatattaa aaatagcagc
      241 aacaacaaat caggcttgct gtgcgtgtat tccttttaat ggaatttata ataaattctt
      301 attttattat ttgatgtcgc agaaagcaga atttcagaaa aaaagtgagg gaagtgggca
      361 accaaatata tcaaaggaaa aaattatcaa ttatttattt cctttaccac caatccacga
      421 acaacatcgc attgttcaaa aaattgaaca attatttgca gagattgaaa aattgtagga
      481 gtcatcatcg ctttcttggg cgtgttggat acgccctaca gtcactaaat tattgatctt
      541 ttatcatatc ggttttcaac caacctaaaa ggaaacctat atgatccatc tatttgaaaa
      601 gcaccttaat caatctaatc ttgctgaaaa tacccaatca gcttatcttt ttgcggtaaa
      661 acaatatcac aaaaaatttg atcagttatc taaaatcaat ttacagcgat ataaggtatt
      721 cttaattgaa aactataagc ctcagactgt taatttaagg ctaagagcca taaattatta
      781 tctggaattt attaaaaaag aaaaatggaa attatccttt gttaaagttc aacaaaaacc
      841 atttttggaa aatgtaatta gtgaagctga ttatacttat tttaaaaaat gcctaaaaaa
      901 agataacgaa ctatactggt attttgtgat tcgttttatg gcagcaacag gttcgagagt
      961 aagtgaatta atccagataa aatgtgaaca tattaaaata ggctatttag acctctattc
     1021 taaaggtgga aaattaagac gaatttacat cccaaaatct ctgca
//
LOCUS       DANJWC010000265         1047 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.266, whole genome shotgun sequence.
ACCESSION   DANJWC010000265 DANJWC010000000
VERSION     DANJWC010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.266"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(25..>1047)
                     /locus_tag="PWX02_002404"
     CDS             complement(25..>1047)
                     /locus_tag="PWX02_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730364.1"
                     /translation="KEFLDKGFIPNDNYRDIIALLPVALYVFHLRSLKHIKNLESNSY
                     LDEEGRDTVNKYKAFFSIFEAEIRDATCICDNKIVTINGEVCCDIKEVARKIVIEFLN
                     EVDIGNKVKFYYPDERHELFVELLIVLATLRIYRNRNIFFFEKGFFNFFPEFITILGT
                     GAFNGFLDFPSLVGKVFPEIIDTINEGLDLDIAIENFEVLSQEEMTECMGLYEQALIK
                     KAEEGEIGLLNVDEINSFYEDNSELILLAKQPMVEEIIPKPDSKASSAILKFFDSNSN
                     TLIAKLKESGLQLSAVALQNDQNIARVANVVYNLLPGMVRIFVSYDTVENFLLNNRQW
                     LINKLV"
BASE COUNT          348 a          206 c          127 g          366 t
ORIGIN      
        1 tatccatagt tcttcccctc caaattaaac taatttatta atcaaccatt ggcggttatt
       61 taataagaaa ttttctacgg tatcgtaact tacgaaaatt cttaccatgc ccggcaataa
      121 gttataaacg acatttgcta ctcgtgcgat attttggtcg ttttgtaatg ccacagcact
      181 taattgcaaa ccactttctt ttaatttagc aattaaggta tttgagtttg aatcaaagaa
      241 ttttagaatt gcagaggagg ctttgctatc tggtttaggg attatctctt caaccatagg
      301 ttgtttagca agtaagatta gctcactatt atcttcataa aagctattta tttcatcaac
      361 atttaataaa ccaatttcgc cttcttcagc tttcttaatg agagcctgct catataaacc
      421 catacattca gtcatttctt cctgactcaa cacttcaaag ttctcaatcg caatatctaa
      481 atctaaaccc tcatttatcg tatcaataat ttctggaaaa acctttccta ccaaactagg
      541 gaagtctaaa aatccattaa acgcaccagt cccgagaata gtgataaatt cgggaaaaaa
      601 gttaaaaaac cccttttcaa agaagaatat atttctatta cgatatattc ttaatgttgc
      661 tagaacaatg agcagctcta caaaaagctc atgtctttca tctgggtaat aaaacttaac
      721 tttattacca atatccactt catttagaaa ttctataaca attttccttg ctacctcctt
      781 aatatcacag cacacctcac cattaattgt tacaatttta ttgtcacaaa tacatgttgc
      841 atctcgaatc tctgcttcaa aaatagagaa aaatgccttg tatttattta ccgtatctct
      901 tccttcttca tctaaataac tgttagattc tagatttttg atatgtttca aagatcttaa
      961 gtgaaagaca tataacgcta ctgggagtag agcaataata tctctataat tatcattagg
     1021 tataaatcct ttatctaaaa actcttt
//
LOCUS       DANJWC010000266         1033 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.267, whole genome shotgun sequence.
ACCESSION   DANJWC010000266 DANJWC010000000
VERSION     DANJWC010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.267"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..274
                     /locus_tag="PWX02_002405"
     CDS             <1..274
                     /locus_tag="PWX02_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sialyltransferase"
                     /protein_id="HDL1730365.1"
                     /translation="LIIMENISFPAEDFISQLKPRKVLSIASTSLVYTTLISKDIKAI
                     SIYPLFRKEVLKKIEYKEEYFKDIESHYSLLSKFDGIRILNNTNEI"
     gene            264..>1033
                     /locus_tag="PWX02_002406"
     CDS             264..>1033
                     /locus_tag="PWX02_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730366.1"
                     /translation="MKYSNYKILVVSTYDSFLKVGINLARRINHSSIEVAIQYVKKNQ
                     LSIRQLVESGANNYRVFDYKILNNSDFSKYDIVIAALGNIASQKLITRLYSLNKRPLI
                     ITCFSGSIFGNAESISSRINSDILLVNNESDRNIAQSIASEYGLETTILNYGLINLDL
                     SFQRNKKSGKNIFFIDQVKIPQLKTEREYVLEKLLELARSKPDYSVFLKTRLYGNEIT
                     VHKDQYPYAHLLRNKKNIPTNFSLYNDSIEVAFQEMDI"
BASE COUNT          392 a          122 c          150 g          369 t
ORIGIN      
        1 tctgattatt atggaaaata tttcttttcc tgcggaggat tttatatctc aattaaaacc
       61 aagaaaagtt ctatctattg cctctacatc tcttgtttat actactttaa tatcaaaaga
      121 tattaaggca atttctatat atcctctatt tagaaaagaa gttctaaaga aaatagagta
      181 taaagaagaa tattttaaag atatagagag tcattattca ttactaagta agtttgatgg
      241 tattagaata ttaaataata caaatgaaat atagtaatta taaaatatta gtagtttcaa
      301 cctatgattc ttttttaaaa gtaggaatta atcttgccag aaggattaat catagtagta
      361 ttgaagttgc aatacaatat gtaaaaaaga atcaactttc tattcgtcaa ttagttgaaa
      421 gtggagcaaa taactatcgt gtctttgatt ataaaatatt gaataattcg gatttttcaa
      481 agtatgatat agttattgca gcactaggta atatagcctc gcagaaacta attacacgtt
      541 tgtattcctt aaataaaaga cctctgatta ttacctgttt ctcagggagt atttttggta
      601 atgcagaaag tatatcatct cgtattaatt cagatatttt actcgtaaat aatgagagtg
      661 atagaaatat tgctcaatct atagcatctg agtacggttt ggaaactact attttaaatt
      721 atgggctaat taatcttgat ttaagttttc aacgaaataa gaaatctggt aaaaatattt
      781 tttttattga tcaggtaaaa attccacagc taaaaactga aagagaatat gtattagaaa
      841 aacttctaga actagctaga agtaaacctg attatagtgt tttcttaaaa actcgtttat
      901 atgggaatga aataactgtt cataaagatc agtaccctta cgcacattta ctaagaaata
      961 aaaaaaatat tcctacaaat tttagtttat ataatgattc cattgaagtt gcttttcagg
     1021 aaatggatat ttg
//
LOCUS       DANJWC010000267         1028 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.268, whole genome shotgun sequence.
ACCESSION   DANJWC010000267 DANJWC010000000
VERSION     DANJWC010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.268"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            2..313
                     /locus_tag="PWX02_002407"
     CDS             2..313
                     /locus_tag="PWX02_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDL1730367.1"
                     /translation="MAKSLDAKREELTNEIRDILKNAEELFNETSDSSTEEYKKLKDK
                     LAKQFDDVKDRFATLKDDTVDSARTVVKQTDTLVQDNPYKAITVAGVVGLLLGVLISR
                     R"
     gene            393..788
                     /locus_tag="PWX02_002408"
     CDS             393..788
                     /locus_tag="PWX02_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL1730368.1"
                     /translation="MLDNIKSGFRNSLITLLEIAQVRGEMARIEINEQKNQLISVLIF
                     ILLAFVFLLVSFVSLLFGLDNYLLPEQKVKVFFSIAIIMFIAVTICGMMILSALKKQR
                     HFMQSTLTELKLDIAAFKSALTLKQNGEH"
     gene            775..>1028
                     /locus_tag="PWX02_002409"
     CDS             775..>1028
                     /locus_tag="PWX02_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730369.1"
                     /translation="MENIKMEELKLTEKELLVLKGNALRMRFKAETNKTKNEIMQPFK
                     QVKQTGLALVNSPAVKTIALDFLVKRLLSMKGISFSVLGLT"
BASE COUNT          366 a          127 c          193 g          342 t
ORIGIN      
        1 tatggctaaa agcttagatg caaaacgtga agagttaaca aacgagattc gcgatatttt
       61 gaaaaatgca gaggaattat ttaatgaaac atctgattct tctacggagg aatataaaaa
      121 gttaaaagat aaattagcaa aacagtttga tgatgtaaaa gatcgttttg caacattaaa
      181 agatgatacc gttgatagtg caagaactgt agttaaacaa actgatacct tagttcaaga
      241 taatccgtat aaagcaatta ccgttgccgg tgtggtaggg ttattattag gtgttttaat
      301 ttctagaaga taattatctg taattcgtca ttaagtcatt aagccacgcc atatttgggg
      361 tggcttatta tttaatataa ggaatggtta taatgttaga taatattaaa tccggattta
      421 gaaatagtct cattacatta ttggaaatag ctcaagtgcg tggtgagatg gcaagaattg
      481 aaattaatga gcaaaaaaat caattaattt cagtgcttat atttatctta ttggcttttg
      541 tatttttatt agtttctttt gtgagtttat tatttggttt agataattat ttgttacccg
      601 aacagaaagt taaagtattc ttttctattg ccattattat gtttattgct gtgactattt
      661 gcggaatgat gatactttct gccttgaaaa aacaacgcca ttttatgcaa agtacattaa
      721 ccgagctaaa attagatatt gccgcattta agagtgcttt aactttgaaa cagaatggag
      781 aacattaaaa tggaagagct taaattaacc gaaaaggaac ttctagttct aaaaggtaat
      841 gcacttagaa tgcgatttaa agcggaaacc aataaaacca aaaatgagat tatgcagcct
      901 tttaaacaag tgaaacaaac agggcttgca ctggtcaatt caccggcagt taaaaccatt
      961 gcattagatt ttttggtaaa aagattattg agtatgaaag gaattagctt ttctgtattg
     1021 gggttaac
//
LOCUS       DANJWC010000268         1018 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.269, whole genome shotgun sequence.
ACCESSION   DANJWC010000268 DANJWC010000000
VERSION     DANJWC010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.269"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..860)
                     /locus_tag="PWX02_002410"
     CDS             complement(<1..860)
                     /locus_tag="PWX02_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611716.1"
                     /note="has 3'-5' exonuclease, 5'-3' exonuclease and
                     5'-3'polymerase activities, primarily functions to fill
                     gaps during DNA replication and repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDL1730370.1"
                     /translation="MATIAKNPLVLVDGSSYLYRAFHAFPPLTNRLGEPTGAMYGVLN
                     MLKSLISQVNPSHIAVVFDTKGKTFRYELFKQYKSHRPPMPDDLRPQIEPLHRIIKAL
                     GIPLISIEGVEADDVIGTLAVQAAKDGKDVLISTGDKDMAQLVNDHIMLINTMNNTLL
                     DREGVIEKYGIPPELIIDFLALMGDSADNIPGVKGVGEKTALALLQGIGSLEQIYANL
                     DQIATLSFRGAKNFAPKLEAEKANADLSYLLATIKTDVELDVTHDQLLTQPQDRAELA
                     QLFEHYEFKR"
BASE COUNT          302 a          219 c          206 g          291 t
ORIGIN      
        1 cagcgtttaa attcataatg ctcaaataat tgagcaagct cagcacggtc ttgcggctgt
       61 gttaaaagtt gatcgtgagt aacatcaagt tccacatcgg ttttgatggt agcaagcaaa
      121 taggataaat cggcattcgc tttttccgct tccaattttg gggcgaaatt ttttgcccca
      181 cggaaagaaa gggtggcaat ttgatccaaa ttagcataaa tctgttcaag tgaaccaatg
      241 ccttgcaata aagctaatgc ggtcttttcc cccacacctt ttacgcccgg aatgttatcg
      301 gcggaatcgc ccattaatgc cagaaagtcg ataatcaact caggcggaat gccatatttt
      361 tcaatcacgc cttcacgatc taacaaagta ttgttcatcg tgttaatcaa cataatgtga
      421 tcgttcacca attgtgccat atctttatcg ccggtgctga tcaacacatc tttgccgtct
      481 ttagcggctt gcacagcaag cgtgccgatc acatcatcag cttccacacc ttcaatcgaa
      541 atcagtggaa tgcctaaggc tttgataatg cgatgcaaag gctcaatttg agggcgtaaa
      601 tcatcgggca ttggtgggcg gtgcgattta tattgcttaa acaactcata acggaaggtt
      661 ttccctttgg tatcaaacac cacagcaata tggcttggat tcacttgtga aatcaggctt
      721 tttaacatat tcagcacgcc atacattgcc cctgtcggtt cgcctaagcg attagtgagc
      781 ggagggaaag cgtgaaatgc tcgatataaa taggaagaac catccaccaa taccagtgga
      841 ttttttgcga tagttgccat aaaaatctct ttataatcaa ataaatattg gcgttattat
      901 acaagaatag gcgaaaaata ggataaaaaa agcgtatttc tgctattgca aaaatacgct
      961 tcattttact gggaaattat gattattgcc cgagtgagcc tgctcccatc acaatcat
//
LOCUS       DANJWC010000269         1014 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.270, whole genome shotgun sequence.
ACCESSION   DANJWC010000269 DANJWC010000000
VERSION     DANJWC010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.270"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(164..796)
                     /locus_tag="PWX02_002411"
     CDS             complement(164..796)
                     /locus_tag="PWX02_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2625 family protein"
                     /protein_id="HDL1730371.1"
                     /translation="MKNLEQLLDKQSAWLILRDWFTQAKNHYEILASYPEDAGSALVG
                     MQLSTRSPLGAMVYETGGVFIDHGWLRILGSGNEKLPRGLFDWNFGKTFKQSGEQPSH
                     LLVADDAIGGYFAINAGGIGSDLGKVYYYHPKAKKWENTQLGYSEFLGWALAGDLNHF
                     YQDLRWENWQTDVEKLQGHQVLDLTSKIAVPIERHYQGEFAPENMGYSVA"
     gene            complement(850..>1014)
                     /gene="hscB"
                     /locus_tag="PWX02_002412"
     CDS             complement(850..>1014)
                     /gene="hscB"
                     /locus_tag="PWX02_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248386.1"
                     /note="J-type co-chaperone that regulates the ATPase and
                     peptide-binding activity of Hsc66 chaperone; may function
                     in biogenesis of iron-sulfur proteins; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HDL1730372.1"
                     /translation="LKQIKAEQKAVLEQLEQTLNTADWQKANALTDKLRYFKKLIIQI
                     EKVEEKFFDY"
BASE COUNT          295 a          206 c          170 g          343 t
ORIGIN      
        1 ataaaattgc tttcattttt tattcctctt ttttaattta gtataaaagg aataaaaaat
       61 ataatttgag cgatttttgt gatctaaatc gaaaatcaga aaatttcttt cgttctaaaa
      121 aaataagcta aacagcctat atttcagcct ctcttacaaa cacttaagcc accgaatatc
      181 ccatattttc aggggcaaat tcgccttggt agtggcgttc aatcggaacg gcgattttac
      241 ttgtaagatc cagcacttga tgcccttgta atttctctac atcggtttgc cagttttccc
      301 aacgcaaatc ctgataaaaa tggtttaaat ctccggctaa tgcccagcct agaaattctg
      361 aataacctaa ttgggtgttt tcccattttt tagcttttgg atgataataa taaactttgc
      421 caagatctga gccgattccg ccggcattaa tggcaaaata gccaccgatt gcatcatccg
      481 ctaccagcaa gtggcttggt tgttcgccag attgtttaaa agttttgcca aagttccaat
      541 caaataagcc tcgaggcaat ttttcgttgc ctgagcctaa aatgcgtaac caaccgtggt
      601 caataaatac gccaccggtt tcatacacca ttgcccctag cggtgagcga gtagaaagtt
      661 gcataccaac caatgcactg ccggcatctt cagggtaact cgctaaaatt tcgtaatgat
      721 ttttggcttg ggtaaaccag tctcttaaaa ttaaccaagc cgattgttta tctaataatt
      781 gttctaaatt tttcatttgc aaaatttcct tagaatttga ccgcttgcga aaaccataca
      841 aatgtaggat taatagtcaa agaatttttc ttctactttc tcaatctgaa taattaattt
      901 tttaaagtaa cggagcttgt cggttaacgc attggctttt tgccagtcgg ctgtatttaa
      961 cgtttgttca agctgttcta atactgcttt ttgttccgct ttaatctgtt ttaa
//
LOCUS       DANJWC010000270          986 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.271, whole genome shotgun sequence.
ACCESSION   DANJWC010000270 DANJWC010000000
VERSION     DANJWC010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.271"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..644
                     /locus_tag="PWX02_002413"
     CDS             <1..644
                     /locus_tag="PWX02_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL1730373.1"
                     /translation="KSIDEQLKNGDYYIIKSLDFRPRRVFERSAEKKKIHNLISRAHY
                     DMATNEDSASEHLELFGEDIAEDSFDYPKPEKLIQFVLEASTEPYDLVLDSFLGSGTT
                     AAVAHKTNRRYIGIEIGEHAKTHVVPRLKKVIEGEQGGISKAVNWQGGGSFRFCELGE
                     EVFDAFGSLNPNIRFDDLAAHIWYLENHFPLQKNSEKSPLVGTFNGKAYYFRR"
     gene            682..828
                     /locus_tag="PWX02_002414"
     CDS             682..828
                     /locus_tag="PWX02_002414"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730374.1"
                     /translation="MAELPYFAEFIQQGMDIVVYGEACRLGEVQLAEKKITFKQIPYD
                     VMSR"
     gene            844..>986
                     /locus_tag="PWX02_002415"
     CDS             844..>986
                     /locus_tag="PWX02_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction endonuclease subunit R"
                     /protein_id="HDL1730375.1"
                     /translation="MKLKNYQNEALNRVSHYFSACLQSDAKTAFSQIQPEYSYKIPSE
                     HSN"
BASE COUNT          338 a          163 c          205 g          280 t
ORIGIN      
        1 aaaaaagtat tgatgagcaa ttaaaaaatg gtgattacta tattataaaa agtttagact
       61 ttcgaccaag gagagtattt gagcgaagtg cggaaaagaa aaaaattcat aacttaatta
      121 gcagagctca ttatgatatg gcaacaaatg aagactctgc tagtgaacat ctagaattat
      181 ttggggagga tattgcagaa gattcatttg attatcctaa acctgaaaaa ttaatccaat
      241 ttgtattaga agcatcgact gaaccatatg atctcgtcct cgacagcttt cttggctcag
      301 gtacaactgc agcagttgct cataaaacga accgtcgtta tatcggtatt gagattggcg
      361 aacatgctaa aacccatgtt gtcccacggt tgaaaaaagt aattgaaggt gagcaaggcg
      421 gtatttcaaa agcagtaaat tggcaaggtg gtggatcgtt tcgcttttgt gagttaggcg
      481 aggaagtatt tgacgcattc ggttcactta atccgaacat tcgttttgac gatttagccg
      541 cccatatttg gtatttagaa aatcatttcc ctttgcaaaa aaacagcgaa aaatcaccgc
      601 ttgtcggcac tttcaacggc aaagcctact attttaggcg ataaacgtcc gcagagtggt
      661 aatgttctga cacgaaaatt aatggcggag ttaccatact ttgctgaatt tattcaacag
      721 ggaatggata ttgtggtata tggcgaagcg tgccgtttag gagaagtgca gttagctgaa
      781 aagaaaatta catttaaaca gattccatat gatgtaatgt ctcgttaata gtaggagaaa
      841 aaaatgaaat taaaaaatta ccaaaatgag gcattaaacc gtgtcagcca ctatttttcg
      901 gcttgtttgc aatctgatgc aaaaaccgca tttagccaaa ttcaacctga atatagttac
      961 aagatcccaa gtgaacacag taattt
//
LOCUS       DANJWC010000271          950 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.272, whole genome shotgun sequence.
ACCESSION   DANJWC010000271 DANJWC010000000
VERSION     DANJWC010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.272"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            50..>950
                     /locus_tag="PWX02_002416"
     CDS             50..>950
                     /locus_tag="PWX02_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730376.1"
                     /translation="MLSEKIDVLINTAKILYKKSRFSSCVDVCNIFIEEGNSSYYIYE
                     LLGECYYRLNMLDLSLNSFKNALELNNKLVSAKFKSIVLKHRLHRNLTIKEITEIESV
                     AVKRNNMNWLRSVSYIQYSYGMYDECFKNLKRLEESDSIIYSLDYMTMAECCKRDNEY
                     EKYNLKELDNTFYYKYENNKSSTLLVTLSATNNFALQKYEYSSDKLFLTDNSHSYYML
                     SYNKIGKLIEKLCNDNNYRKICIVGLSKGGTGALYLFDYIQNHIPSVEIRCIAFSPQV
                     NIYPFNRNLNIPSYRKLQEYIDCN"
BASE COUNT          373 a           90 c          148 g          339 t
ORIGIN      
        1 gactcagtaa ttatggaaaa atttaaaatt ctatatagga aaacattgta tgctatcaga
       61 aaaaattgac gttttaatta atactgcaaa gattttgtat aaaaagagta ggttctcttc
      121 ttgcgtggat gtatgtaata tatttattga agaaggtaat agtagttatt atatttatga
      181 gctactagga gaatgctatt atagactaaa tatgttagat ttgtctctta attcatttaa
      241 aaacgcttta gaactaaaca acaaattagt tagtgctaaa tttaaaagta ttgtattaaa
      301 acatagatta catagaaatt taaccataaa agaaattact gaaatagaga gtgttgcagt
      361 aaaaagaaat aatatgaact ggcttcgttc agtatcttat attcaatata gttatggaat
      421 gtacgatgaa tgttttaaga atttaaaaag attagaggag tcagatagta ttatttattc
      481 tcttgattat atgacaatgg cagagtgctg taaaagagat aacgaatatg aaaaatataa
      541 tttgaaagaa ttagataata ctttttatta taagtatgaa aataataaat catcaacatt
      601 attagttaca ttatctgcaa caaataattt tgcattgcaa aagtatgagt attcttctga
      661 taagttgttt ttaacagata attctcactc ttactatatg ctatcgtata ataaaattgg
      721 taagcttata gagaaattat gtaatgataa taattataga aaaatatgta ttgtaggttt
      781 atctaagggg ggaacagggg ctttgtatct cttcgattat attcaaaatc atattccaag
      841 tgttgagata agatgtattg ctttctctcc tcaagtaaat atatatccgt ttaatagaaa
      901 tctaaatatt cctagctata ggaaattaca agaatatatt gattgtaata
//
LOCUS       DANJWC010000272          896 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.273, whole genome shotgun sequence.
ACCESSION   DANJWC010000272 DANJWC010000000
VERSION     DANJWC010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.273"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            131..>896
                     /locus_tag="PWX02_002417"
     CDS             131..>896
                     /locus_tag="PWX02_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL1730377.1"
                     /translation="MLNWAGKSTAYQTLQTPTSKTLTPCEEESVDFENTQNIFIEGEN
                     LDVLKALQKSYFNSVKMIYIDPPYNTGNDFIYNDSFADTKADYAEKVGDVDEHGKLKR
                     AFVRNSKENGHYHSNWLNMMLPRLHLAKNLLRDDGVIFISIDDNEQAQLKLLCDEVFG
                     EENFVAILSVENNPKGRKNSKFISVSNDFCIIYAKNKEMGKFVENIPKNAKDMCQDEN
                     GHYVHASGKRVLVGENKFNQPVTDFTSDKHYSVYFNK"
BASE COUNT          313 a          150 c          169 g          264 t
ORIGIN      
        1 ttcgcttaaa gatcaacaac tttctcaatt aaaatccctt ttacctgaaa ttttcagcga
       61 aggtaaaatc gacttcgaca aattccaaca acttttttcg gataattttt ccaccgaacc
      121 tgaccgctat atgctcaact gggcaggaaa atcaacagcc tatcaaacct tacaaacccc
      181 aaccagcaaa acgctcactc cttgcgagga agagagtgtg gattttgaaa atacgcagaa
      241 tatctttatt gagggtgaaa atttagatgt attaaaagcc ctgcaaaaat cctacttcaa
      301 ttcggtcaaa atgatttata tcgacccgcc atacaataca ggcaacgatt ttatttataa
      361 cgacagtttt gccgatacca aagcagatta tgcggagaaa gtgggcgacg ttgatgaaca
      421 cggcaaattg aaacgagcct ttgttcgtaa cagcaaagaa aatgggcatt atcacagtaa
      481 ctggctgaat atgatgttgc ctcgtttgca tctcgcaaaa aatttactgc gtgatgacgg
      541 ggtgattttt atttcaattg atgataacga acaggcacag ctgaaattgc tttgtgatga
      601 agtgtttggg gaggagaatt ttgttgccat attatcagtt gaaaataatc cgaaaggtag
      661 aaagaattct aaatttattt ctgtaagtaa tgatttttgt attatttatg ctaaaaataa
      721 agaaatgggg aaatttgttg aaaatattcc taaaaatgct aaggatatgt gtcaagatga
      781 aaatggacat tatgttcacg caagtggaaa acgagtatta gttggggaaa acaaatttaa
      841 tcagcctgta acagatttta cctcagataa acactattca gtttatttta ataaat
//
LOCUS       DANJWC010000273          889 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.274, whole genome shotgun sequence.
ACCESSION   DANJWC010000273 DANJWC010000000
VERSION     DANJWC010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.274"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..748
                     /locus_tag="PWX02_002418"
     CDS             <1..748
                     /locus_tag="PWX02_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HDL1730378.1"
                     /translation="FSIIDVIAILTESLDPSAYWRKLKQRLKAEGNETVTNCHGLKMP
                     SADGKMRLTDVADTEQLFRLIQSIPSPKAEPFKQWLAKVGYERLQEINDPSQSLDRAR
                     ENWQKIGRSEKWIQQRMMGQETRNKLTDYWKSSGVEQPQDFAFLTNVIHQEWSGLSVQ
                     KHKSLKGLKSQNLRDHMSEAELLFTALAELSSRQIAESMQAVGVDENAQAGKQGGNIA
                     KNARQELENKTGKSVVTGENFLPPAKNEEE"
BASE COUNT          313 a          169 c          194 g          213 t
ORIGIN      
        1 tttttctatt attgatgtga ttgctatttt aaccgaaagc ctagatccct ctgcttattg
       61 gcgtaagcta aaacagaggc taaaggcaga aggcaatgaa accgtgacaa attgtcacgg
      121 gttgaaaatg ccctctgctg acggaaaaat gcgtttgact gatgtagcag ataccgaaca
      181 acttttccga cttattcaat ctatccctag tccgaaagca gagcccttca aacaatggtt
      241 agcaaaagta ggttatgaac gcttacagga aataaacgat ccaagtcaaa gtctagatcg
      301 agcaagggag aattggcaga agataggacg aagtgaaaaa tggattcagc aaagaatgat
      361 gggacaagaa acccgaaata aactgaccga ttattggaaa tcgtcagggg ttgaacagcc
      421 acaagatttt gcatttttaa ccaatgtgat tcatcaagaa tggagcggac tatctgtaca
      481 aaagcataaa tcgcttaaag gtttaaaaag ccaaaatctg cgagatcata tgagcgaagc
      541 tgaattgtta tttacagcac ttgctgaact ttccagtcgg caaattgcag aaagtatgca
      601 ggcagttggg gttgatgaaa atgcccaagc aggaaaacaa ggtggcaata ttgccaaaaa
      661 tgcccgtcag gaattggaga ataaaacagg taaatcagtt gttaccggag agaatttttt
      721 acctccagct aaaaatgaag aagaatagac acaatagatg ttcttaatta gtcaaggtcg
      781 agcctccccc aaccttgacc acaaacttgc aaaaaatagc caaaatcaca ccgcttgcaa
      841 gttaaataga ttgtgccgct accgcatttt ggtagccgaa ttgtcgcca
//
LOCUS       DANJWC010000274          837 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.275, whole genome shotgun sequence.
ACCESSION   DANJWC010000274 DANJWC010000000
VERSION     DANJWC010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.275"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            3..>837
                     /locus_tag="PWX02_002419"
     CDS             3..>837
                     /locus_tag="PWX02_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL1730379.1"
                     /translation="MSSLQHSLTKTEKKIAAAILSQPELLSQCSLSEVAKQLDVGEAT
                     FIRFCRTLGFKGYTDFKLELAIELATNQDNRVLLDTDVSESDTSKDIAEKLKVSLDNV
                     IEETINLLDFNVLEKVVEELRKAKRIFLFGVGSSGLTAEDAKHKLMRIGLQTDAVTNN
                     HFMYMQAALVKEGDLVIGISHSGYSEEIVKSLRFSRANKATTVAITHNLRSPVTEEAN
                     YVLINGNRQGHMQGDSIGTKMSQLFVLDLIYTLLVKAEPENALKQKQKTLNVILEQRV
                     KG"
BASE COUNT          277 a          128 c          177 g          255 t
ORIGIN      
        1 caattagctc gctacaacat agtttaacca aaacagagaa gaaaatagct gctgctattt
       61 tatctcagcc ggaattactt agccaatgtt cattatctga agtggccaaa caattagatg
      121 ttggtgaagc aacttttatt cgtttttgtc gtacattagg ttttaaaggt tataccgact
      181 ttaaattaga gcttgcaata gagttggcta caaatcaaga taatagagta ttgcttgata
      241 cggatgtttc agaatcggat acatcgaaag atattgctga gaagctcaaa gtgagtttag
      301 ataacgttat tgaagagact attaatttgc ttgactttaa cgtgttggaa aaagttgttg
      361 aagaattacg taaggcaaaa cggatctttt tatttggagt gggttcttct gggttaaccg
      421 cagaagatgc aaaacataaa ttaatgcgta ttgggctaca aaccgatgca gtcacaaata
      481 atcattttat gtatatgcag gcggcattgg tgaaagaggg ggatttagtt atagggatta
      541 gtcattcagg ctattctgaa gaaattgtga aatcactacg tttttctcgt gcaaataaag
      601 cgacaacggt tgcgattaca cacaatttac gttcccccgt cacggaagag gctaattatg
      661 tgttaattaa tggtaatcga caggggcata tgcaaggaga ttctatcggt actaaaatgt
      721 cacaactttt cgttcttgat ttaatttata ccttactagt taaagccgaa cccgaaaatg
      781 cgttgaaaca gaaacaaaaa acactgaatg tgattttgga acaacgtgtt aaaggct
//
LOCUS       DANJWC010000275          832 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.276, whole genome shotgun sequence.
ACCESSION   DANJWC010000275 DANJWC010000000
VERSION     DANJWC010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.276"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            31..>832
                     /locus_tag="PWX02_002420"
     CDS             31..>832
                     /locus_tag="PWX02_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL1730380.1"
                     /translation="MWVRLGDICLKITDGTHHSPPNIDKGDFLYITAKNIKKDGLDLS
                     KISYVTKEIHNEIFSRCNPEKGDILYIKDGATTGVSIINTLNEPFSMLSSVALIKTSQ
                     EIDNEYLNYVMNSHYFYNISIGSMSGTGIPRITLTKLESYLVPVPPLLEQQRIVQKIE
                     ELLPLVERYEQTEQQLTKLNNTFPEQLKKSVLHAAIQGKLTEQDPNDELASCLIERIK
                     AEKNRLIAEKKLKKPKSVSEIVMRDNLPYEIKAGQERCIADEVPFEIPQ"
BASE COUNT          302 a          141 c          157 g          232 t
ORIGIN      
        1 gaaccacctt ttgagattcc tgagagttgg gtgtgggtga ggttggggga tatatgccta
       61 aaaattacag atggaactca tcattctccc ccaaatatcg ataagggtga ttttctttat
      121 attacagcta aaaatataaa gaaagatgga ttagatttat caaaaattag ctatgtaaca
      181 aaagaaattc ataatgaaat tttcagccgt tgtaatcctg aaaagggaga tattctatat
      241 attaaagatg gagccacaac tggagtaagt attataaata ctcttaatga gccatttagt
      301 atgttatcca gtgttgcctt aattaagacc tcacaagaaa tagataatga gtacttaaat
      361 tatgtaatga atagtcatta tttttataac atttctattg gaagtatgtc tggaacaggt
      421 attccaagaa taacattaac taaattagaa tcttatttag tcccagttcc gccactccta
      481 gaacaacagc gcattgtgca aaaaatcgag gaattattac cgcttgttga acgctacgag
      541 caaaccgaac agcaactcac taaactcaac aatacattcc ccgaacagct gaaaaaatcg
      601 gtgttacacg cagctattca gggtaaatta accgagcaag atccgaatga tgaactggca
      661 tcttgcctga ttgagcggat aaaagcggag aaaaatcgtc ttattgccga gaaaaaactc
      721 aaaaaaccaa aaagtgtttc agagattgta atgcgtgata atttacctta tgagatcaaa
      781 gcaggtcagg aacggtgtat cgccgatgaa gtaccatttg agattcctca aa
//
LOCUS       DANJWC010000276          831 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.277, whole genome shotgun sequence.
ACCESSION   DANJWC010000276 DANJWC010000000
VERSION     DANJWC010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.277"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            1..831
                     /locus_tag="PWX02_002421"
                     /pseudo
     CDS             1..831
                     /locus_tag="PWX02_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011150324.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT          261 a          143 c          200 g          227 t
ORIGIN      
        1 gatgaatcca cagaaaaagc ggttgtacaa ttcgctttag ataatcctgc ttttggtcaa
       61 gttcgagtga gtaatgaact gcgtaaacag gggatttttg tctctgcagg tggtttttgt
      121 ctctgcaggt ggtgtacgtt ccatttggtt gagacatcac ttagctaatt ttaagcaacg
      181 cttaactgcc ctcgaaaaat tggtggcaga gcaaggcatt attctgagtg agccacagat
      241 acaagccttg gagcggaaga aagaagatga gattgcctgt ggtgaaattg aaacggcaca
      301 ccccggctat ttagggtcac aagatacctt ttatgtcggc aatattaaag gtgtgggtcg
      361 tatttatcag caaacgttta ttgatactta tagtaaagtg gcatttgcaa aactgtatac
      421 aatgaagacg gctatcactg ctgctgatat gctaaatgac aaagtattac cattctttga
      481 aagccagagg ttacctatgt tacgagtgct gacagaccga ggcagtgagt attgtgggaa
      541 agttgagaat cacgattatg agctttattt ggctatcaat gacatagagc acacaaaaac
      601 caaagtgaaa cagccacaaa caaacggcat ctgtgagcgt tttcacaaaa cgattttgca
      661 ggagttctac caagtggcat tcagaaaacg gatttatatg gatttagagg cattgcaagc
      721 tgacttagat gaatggttaa tgtattataa ccatcatcga acccatcaag gaaaaatgtg
      781 ctgtggaaga acaccgatgg agacattagt tgatggaaaa cgcatttggg c
//
LOCUS       DANJWC010000277          829 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.278, whole genome shotgun sequence.
ACCESSION   DANJWC010000277 DANJWC010000000
VERSION     DANJWC010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.278"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..>829
                     /locus_tag="PWX02_002422"
     CDS             <1..>829
                     /locus_tag="PWX02_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HDL1730381.1"
                     /translation="SATNIKNELQTKLEVELELIELKNTYNQILLGGSEDLSALYSAQ
                     LDEKKQEQQNLQAEIANLQTAINNKIVEESQNKLDQAQAVQNQQNNATTNPVILRELD
                     INTKVTQELLKQTKDMTQLSQDNLRIKSVLDNLQQTQRNIEEQISSLQGTLVLSRIIN
                     KQKQSLPQDEMISGLSKQIADLRVRVFDITEFKDSFADINAYISRIEQDEKTTFTSKE
                     KEQLSKILQERSDTLTEMIKSLNNQLNLLINIELNQQQAQTISDALQQKLQQQSFWV"
BASE COUNT          337 a          146 c          139 g          207 t
ORIGIN      
        1 tttcagcgac taacattaaa aatgaactac agacaaagtt agaagtagaa ttagaattaa
       61 tagaattaaa aaacacttac aatcaaatat tacttggtgg cagcgaggat ttatctgcgc
      121 tttactctgc tcaattagat gaaaaaaagc aagagcagca aaatctacaa gcagaaattg
      181 caaacctgca gactgctatc aacaataaaa ttgtagaaga gtctcagaac aagctagatc
      241 aagcacaagc ggttcaaaat cagcaaaata atgcaacgac gaacccagtt atcttgcgtg
      301 aattggatat aaataccaaa gtcacacaag agttgctaaa acagaccaaa gatatgacgc
      361 agttatcaca agataatttg cgaatcaaaa gtgtgctaga taatttacag caaacccaac
      421 gtaatattga agaacaaatc agttctctgc aaggtacgtt ggtgctttct cgtattatta
      481 ataagcaaaa acaatcatta ccacaagatg aaatgatttc cggactatca aaacaaattg
      541 cagatttacg tgtacgtgtt tttgatatca ctgaatttaa agactctttt gcggacataa
      601 atgcttatat cagccgcata gagcaagatg aaaaaacaac ctttacgagt aaagagaaag
      661 aacaactgag taaaatttta caggaacgtt ctgacacttt aactgagatg attaaatcat
      721 taaataatca gctcaattta ttaatcaata ttgaactaaa ccagcaacaa gcgcagacca
      781 ttagtgatgc gttacagcaa aaactgcaac agcaaagctt ctgggttaa
//
LOCUS       DANJWC010000278          759 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.279, whole genome shotgun sequence.
ACCESSION   DANJWC010000278 DANJWC010000000
VERSION     DANJWC010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.279"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            32..655
                     /locus_tag="PWX02_002423"
     CDS             32..655
                     /locus_tag="PWX02_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HDL1730382.1"
                     /translation="MRVYFKKSNLKEYVIWPVPTNTKDYYQVEVDSESDLYGKTAVKT
                     KNGIVLVEPAPTSYHKWSGTEWIISPEQQAIKKNEEIAIMRERINALRDEKSAGGVFV
                     EQLGKWFDSDDKAQKKLLGLKATMDLIGTEMTVDWTCADNTDFEGFGKAHLTAVIAAI
                     LQAENHNHTIARQHKAALEQAENPLEYDYSAGWTKTYADYLEEQANV"
     gene            648..>759
                     /locus_tag="PWX02_002424"
     CDS             648..>759
                     /locus_tag="PWX02_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HDL1730383.1"
                     /translation="MYKVYLALYKGSGGSLYDYFTDWLIRKATKGKYSHCE"
BASE COUNT          263 a          135 c          177 g          184 t
ORIGIN      
        1 aattaaaaaa attatacacc taggagcata aatgagagtt tactttaaaa agtcaaatct
       61 taaagaatat gtgatttggc ctgtgccaac taatactaaa gactattatc aagtcgaggt
      121 ggatagcgag agtgatttat atggcaaaac agcggtaaaa acaaaaaatg ggattgtatt
      181 ggtagagcca gctccaacca gttatcacaa gtggagcggg acagaatgga taatcagccc
      241 agagcaacaa gcgatcaaaa aaaacgaaga aattgcaata atgcgagagc gtatcaacgc
      301 cttgcgtgac gagaaatcgg caggcggcgt gtttgttgag caactaggca aatggtttga
      361 ttcagacgac aaagcccaga aaaagctgtt aggcttaaaa gcgacaatgg atttaattgg
      421 cacagagatg actgtcgatt ggacttgtgc cgataatacc gattttgagg gattcggcaa
      481 agctcattta actgctgtca ttgctgcaat tctgcaagcc gaaaaccaca atcataccat
      541 tgctcgccag cacaaggcgg ctttagagca agctgaaaat ccgcttgaat atgattattc
      601 ggcggggtgg acgaaaactt atgctgatta tttagaggag caagctaatg tataaagtct
      661 atttagcact ttacaaaggc tcgggtggta gcctatatga ctatttcaca gattggttaa
      721 ttcgcaaagc cactaaaggc aaatattcgc attgcgaaa
//
LOCUS       DANJWC010000279          756 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.280, whole genome shotgun sequence.
ACCESSION   DANJWC010000279 DANJWC010000000
VERSION     DANJWC010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.280"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..696
                     /gene="tuf"
                     /locus_tag="PWX02_002425"
     CDS             <1..696
                     /gene="tuf"
                     /locus_tag="PWX02_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199348.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL1730384.1"
                     /translation="PGDDTPIVRGSALQALNGVAEWEEKILELANHLDTYIPEPERAI
                     DKPFLLPIEDVFSISGRGTVVTGRVERGIIRTGDEVEIVGIKDTAKTTVTGVEMFRKL
                     LDEGRAGENVGALLRGTKREEIERGQVLAKPGSITPHTDFESEVYVLSKEEGGRHTPF
                     FKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMTVSLIHPIAMDEGLRFAIREGG
                     RTVGAGVVAKIIK"
BASE COUNT          244 a          143 c          184 g          185 t
ORIGIN      
        1 ccgggtgatg acactccaat cgtacgtggt tcagcgttac aagcgttaaa cggcgttgct
       61 gaatgggaag agaaaatcct tgaattagca aaccacttag acacatacat tccagagcct
      121 gagcgtgcga ttgataaacc attcttatta ccaatcgaag acgtattctc aatttcaggt
      181 cgtggtacag tagtaacagg tcgtgttgag cgtggtatca tccgcactgg tgatgaagtt
      241 gaaatcgtag gtatcaaaga tacagcgaaa acaaccgtaa caggtgttga gatgttccgt
      301 aaattattag acgaaggtcg tgcgggtgag aacgtaggtg cattattacg tggtactaaa
      361 cgtgaagaaa tcgaacgtgg tcaagtatta gcgaaaccgg gttcaatcac tccacacaca
      421 gacttcgaat cagaagttta cgtattatca aaagaagaag gtggtcgtca cactccattc
      481 ttcaaaggtt accgtccaca gttctacttc cgtacaacgg acgtaacagg tacaatcgaa
      541 ttaccggaag gtgtagagat ggtaatgcct ggcgacaaca tcaaaatgac tgttagccta
      601 attcacccaa tcgcaatgga cgaaggttta cgttttgcga ttcgtgaagg tggtcgtaca
      661 gtaggtgcgg gcgttgtagc gaaaatcatc aaataattaa gattgtttga taaagcgaaa
      721 agctaaataa aaactagaaa gaggcatatc gaaagg
//
LOCUS       DANJWC010000280          730 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.281, whole genome shotgun sequence.
ACCESSION   DANJWC010000280 DANJWC010000000
VERSION     DANJWC010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.281"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(32..415)
                     /locus_tag="PWX02_002426"
     CDS             complement(32..415)
                     /locus_tag="PWX02_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730385.1"
                     /translation="MHIEDKIAWWLANGETGVSSKTMAFYLGYGIRPKIEGYPHDVSD
                     FRRCFLLLETVPFLRNRIEKMAELGKVWAALAKEWHTLEALYNEEEGQIRCPKTYAKL
                     REILEANEENVVRIGNVSISIGSTT"
     gene            complement(503..679)
                     /locus_tag="PWX02_002427"
     CDS             complement(503..679)
                     /locus_tag="PWX02_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730386.1"
                     /translation="MTEEDKEYLQTKIENEGFEYAFVSYSDFEEVQDEKFHGLRKAYL
                     KARSELAEYIDIED"
BASE COUNT          188 a          164 c          129 g          249 t
ORIGIN      
        1 cctcatttag agggatttgg ttaaataatc gttaagtcgt tgaacctatc gaaatgctca
       61 cattaccgat gcggacaaca ttttcttcgt tggcttctag gatttctctc aacttagcat
      121 aggttttggg gcatcttatt tgaccttcct cttcgttgta gagagcctct aatgtgtgcc
      181 attcttttgc aagtgcagcc caaactttcc caagttcagc cattttttca atcctatttc
      241 gcaaaaaagg gactgtctcc aacaacagaa agcaacgcct gaaatccgat acatcgtgtg
      301 ggtagccttc tatttttggt cttattccgt aaccaaggta aaaagccatt gttttactgc
      361 ttacccctgt ctctccattg gcaagccacc aagcgatttt gtcttctatg tgcattttct
      421 ctccaaaaaa taaccgcttg cggaacaagt tctacaagcg gttgtcaagt gttcatttgt
      481 tcaatgaaca gttcatcagt ggttaatctt caatatcaat atattccgct aactcacttc
      541 gagcttttaa ataagccttg cgaagcccgt ggaatttttc atcttggact tcttcaaagt
      601 cactataact gacgaaagcg tattcaaaac cttcgttttc aattttagtt tgaagatatt
      661 ctttgtcttc ttctgtcatt tcgtttctcc tattgagagt ggataaaaat aaaccctgca
      721 cgggcagggt
//
LOCUS       DANJWC010000281          713 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.282, whole genome shotgun sequence.
ACCESSION   DANJWC010000281 DANJWC010000000
VERSION     DANJWC010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.282"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..634)
                     /locus_tag="PWX02_002428"
     CDS             complement(<1..634)
                     /locus_tag="PWX02_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDL1730387.1"
                     /translation="MLDVVELSRLQFALTALYHFLFVPLTLGLSFVLVVMETLYVTTG
                     KEVYKDMTKFWGKLFGINFALGVTTGITMEFQFGMNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWERLSKAKHLLATYCVAFGSNFSALWILVANGWMQNPIASEFN
                     FETMRMELSSFSELVLNPVTQSKFLHTVTAGYVCGAIFVLGISSYYILKGR"
BASE COUNT          270 a          145 c          112 g          186 t
ORIGIN      
        1 cacgaccttt taagatatag tatgaactaa tacctaatac aaaaatagca ccacaaacat
       61 agcctgcggt tacagtatgt aagaatttag attgtgtaac tgggtttaat acaagttcag
      121 agaaacttga aagctccatt cgcatggttt caaagttgaa ttcagaagca atcgggtttt
      181 gcatccaacc attagcaact aaaatccata gtgcagagaa gttagaacca aacgcaacac
      241 agtaagttgc taataaatgt ttcgctttcg ataaacgttc ccagccaaag aagaacaagc
      301 ccacaaaggt agattctaag aagaacgcca ttaaaccttc aatcgctaat ggagcgccaa
      361 aaatatcacc tacataatga gaataatatg accagttcat accaaactgg aattccattg
      421 taataccagt tgttacccct agggcaaagt taataccaaa taatttaccc cagaatttag
      481 tcatatcttt atatacttct ttacccgtag ttacatacag ggtttccatc accacaagga
      541 cgaaagaaag acctaatgta agcggtacga ataagaagtg atagagtgca gttagagcaa
      601 actgcaaacg tgagagttca actacatcta acatctcaaa cctcttatat taatatacta
      661 atttgacaat tagattactg taaaagtaat cacccacaaa ttcactcaat tct
//
LOCUS       DANJWC010000282          712 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.283, whole genome shotgun sequence.
ACCESSION   DANJWC010000282 DANJWC010000000
VERSION     DANJWC010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.283"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(117..377)
                     /locus_tag="PWX02_002429"
     CDS             complement(117..377)
                     /locus_tag="PWX02_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730388.1"
                     /translation="MPRNELTKNARAIVDLIHRKSATVTHKKLARAIGLSESQFSRTF
                     ADNVEMVAVIIDYLSIELADKDELLELKEKRYKRINLEEAKT"
     gene            complement(398..598)
                     /locus_tag="PWX02_002430"
     CDS             complement(398..598)
                     /locus_tag="PWX02_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1730389.1"
                     /translation="MNLTSYLSDRPRGFKADFAKKLGISKSYLRQVETGYSPMPAYLA
                     KKIEEVTNGEVAKSELRPDLWD"
BASE COUNT          211 a          136 c          114 g          251 t
ORIGIN      
        1 ttgttaaaaa cccaccacgc aagcagcata aaaattgcca ctctaataaa aattgaattt
       61 ttactgtggt agaatgattt gaatttttct aaaaaatttt tcattgttgg taacttctat
      121 gttttcgctt cttcgagatt tattcgctta tatcgtttct ctttcagctc tagtaactcg
      181 tctttatccg caagctcaat acttaagtaa tcaataatca ccgccaccat ttccacatta
      241 tctgcaaatg tacggctaaa ttgtgattca ctcagtccga ttgctcttgc tagtttctta
      301 tgcgtcaccg ttgcggattt tcgatgaatc aaatccacaa tcgctcttgc attttttgtt
      361 aattcattgc gtggcattgc acattccttt gggtaaatta atcccacaga tcggggcgaa
      421 gctcagattt ggctacttcg ccattcgtga cttcttcaat tttcttagca agataggctg
      481 gcattggtga ataccctgtt tcaacttgtc gtaaataaga tttggaaata ccaagctttt
      541 ttgcaaagtc agctttgaaa ccacgagggc gatctgacaa ataactagtt aagttcatta
      601 acactcccgt taagtaaaaa ttagattgag tttagattta actaaataca aagtcaagta
      661 aagtttagct tttgcttgtt taggaaattc taaataaaat aatttaaaaa tg
//
LOCUS       DANJWC010000283          694 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.284, whole genome shotgun sequence.
ACCESSION   DANJWC010000283 DANJWC010000000
VERSION     DANJWC010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.284"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..445
                     /locus_tag="PWX02_002431"
     CDS             <1..445
                     /locus_tag="PWX02_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL1730390.1"
                     /translation="NYSLNHDRRRKPVSVAQRSQQNKLYDYYGATGAIDKVASYIFDG
                     KFILIGEDGGNFFTKKDVAFIVEGKFWANNHVHVLSVDFNLEKYFCYYLNALNLPSMG
                     LINGIAVPKLNQRNLNSILIAIPPISEQHRIVEKIEKLFSEIEKF"
     gene            complement(491..>694)
                     /locus_tag="PWX02_002432"
     CDS             complement(491..>694)
                     /locus_tag="PWX02_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL1730391.1"
                     /translation="ARRYKLDPKIVYPHSFRHRFAKSFLERFNDIAFLADLMGHESIE
                     TTRIYLRKTSTEQQEIVNKIIDW"
BASE COUNT          251 a           94 c          112 g          237 t
ORIGIN      
        1 aaattattct ttaaatcatg atagaagaag aaaaccagta tcagttgcac agcgtagcca
       61 acaaaataaa ttgtatgact attatggagc gactggagca atagataaag tggcaagcta
      121 tatttttgat ggtaagttta ttttaattgg agaagatgga ggtaatttct tcactaaaaa
      181 agacgttgct tttattgttg aaggtaaatt ttgggcaaat aatcatgttc atgtattatc
      241 agttgatttt aatttggaaa aatatttttg ttattattta aatgcattaa atttacctag
      301 tatgggatta attaacggaa tagctgtacc caaattaaat caaagaaacc taaactctat
      361 tttaattgct attccaccta tttctgaaca acatagaatc gttgaaaaaa ttgaaaaatt
      421 attttcggaa atagagaaat tttagaaaaa cttatatgtc caaaggcgtg tgaaacacgc
      481 cccttaaaaa ttaccaatca ataattttat tcacaatttc ctgctgttct gtgcttgttt
      541 ttcgtaaata gatccttgtg gtttctatgc tttcgtgccc cattaaatca gcaagaaaag
      601 caatatcatt aaatcgttct aaaaaacttt ttgcaaaacg atggcgaaat gaatgtggat
      661 aaacaatttt aggatctaat ttatagcgtc tagc
//
LOCUS       DANJWC010000284          675 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.285, whole genome shotgun sequence.
ACCESSION   DANJWC010000284 DANJWC010000000
VERSION     DANJWC010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.285"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
BASE COUNT          238 a           94 c          128 g          215 t
ORIGIN      
        1 tggagtggta aagttatcga ctccgacacg cttggtaata tttgtgagat taaaattaag
       61 gtcgcttttc caaaaatcag agttagcgga agcagataaa atacttagga aaaagtatgg
      121 ggatatgatt aactgattga tttttctcat cattttctcc attaggggga taaagtgcaa
      181 aaactaattt ttagctgaac agctattata tggttcaatc ttatgtataa aataccagat
      241 agcaagtaat tataacggca ttttatgatt aagatttaag cacaaaaaaa taaaaaaatc
      301 tgagagattt gtcacaatta ttgaaaaaag tgatattttt taaatatcac cgaagcccaa
      361 gctttgtatc aaggtttcat cttaatgtta gtgataaaag tgactttttc tgaactttgg
      421 tgtaaaaaat ataagagctg taacaaaaag gccgccaggc aataacctaa cggcattaca
      481 agcggtcata tttggcggaa aatttgctag gctacttaaa atttgcgata tgaaatgttt
      541 agtgccattg agctaaaaca acaaaaaagc ctgtaaacgt tgattttaca ggctttctaa
      601 ggttttttcg tttagctctg ttgaactaaa tttagtcaaa tggtgcctgg tcgagacttg
      661 aacttaatgg cttca
//
LOCUS       DANJWC010000285          666 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.286, whole genome shotgun sequence.
ACCESSION   DANJWC010000285 DANJWC010000000
VERSION     DANJWC010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.286"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..320
                     /locus_tag="PWX02_002433"
     CDS             <1..320
                     /locus_tag="PWX02_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730392.1"
                     /translation="YLEEYNDLGTIRYKFQEENLYGFLRDGATLVANGIVNEPSVDCF
                     SQEIAQFTGCHIFSSLYIAFNTQRSFKSHWDSRDIFAVQMQGKKRWIIHTPTFKKTIV
                     YAS"
     gene            310..>666
                     /locus_tag="PWX02_002434"
     CDS             310..>666
                     /locus_tag="PWX02_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HDL1730393.1"
                     /translation="MHRSKDMPEYAPNLDDVYMDIVLEAGDVLYLPRGWWHDPIPVGE
                     ETVHLAVGIFPAYTHNYLTWVSQNMVEKEIARASLSHYESDKELIAQLAEQTAEYIKD
                     KENYHKFIENFYDQKRV"
BASE COUNT          218 a           90 c          130 g          228 t
ORIGIN      
        1 attatttaga agaatataat gatctaggta ctattcgtta taaatttcaa gaagaaaatt
       61 tatatggttt tttaagagat ggggcaacat tagtcgcaaa tggtattgta aatgaaccaa
      121 gtgttgattg cttctctcaa gagatagctc aatttacagg ctgtcatatt ttttcaagtt
      181 tatatattgc gtttaatact caaagatctt ttaaaagcca ttgggatagt cgtgatattt
      241 ttgcagttca aatgcagggt aaaaagcgtt ggattattca cactccaaca tttaaaaaaa
      301 ccattgttta tgcatcgtag taaagatatg cctgaatatg ctcctaatct tgatgatgta
      361 tatatggata ttgtattaga agctggagat gttctttatt tacctcgtgg ctggtggcac
      421 gatcctattc ctgttggaga agaaactgtg catttagctg taggcatttt tcctgcttat
      481 actcataact atctgacttg ggtatctcaa aatatggttg agaaagaaat tgcccgagct
      541 tctttatcgc attatgagtc agataaagag ttgatagcac agttggcaga acaaacagca
      601 gaatatatta aagataaaga gaattatcat aaatttatag agaattttta tgatcagaag
      661 cgggtt
//
LOCUS       DANJWC010000286          665 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.287, whole genome shotgun sequence.
ACCESSION   DANJWC010000286 DANJWC010000000
VERSION     DANJWC010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.287"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(1..653)
                     /locus_tag="PWX02_002435"
                     /pseudo
     CDS             complement(1..653)
                     /locus_tag="PWX02_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT          179 a          149 c          115 g          222 t
ORIGIN      
        1 ctaaatctca caccattttc gcaacgtttt gagctgttct tttggtgtac caaagttaaa
       61 ccgaaattca cattccttca agaataaagg gaaatttttt cggttaattc cgttatattt
      121 tcggagtact cgcttcgctt gattccaaaa attttcaatg ccattgatat ggttttgttt
      181 ctccgcaaac agttcggaat ggttgattcg ctcgtgatga aattcgctca catcaagggc
      241 atcatagcta cgataggtat ccgtataaac ccagctgtca ggcttgattt tccttgtaat
      301 gaccggcatt agcgtttctg atttgtattt tctaccacta cggtaaacac ctttccttga
      361 cgcttcaaca aaccgaatac agcgactttc ccagccgcac ctcgccctcg ttttcctttg
      421 cgatgaccac caaaatagct ttcatctacc tcaatttttc cctcaaaaat ttcatgagct
      481 tcaagggcta aatggtgttc aatgaccagc ctaattttgt ggtaaaacag ggctgccgta
      541 ttgggttgaa tacctaataa atcagcagcg gttctagcgg tcacttcagc aacaaaaaat
      601 tcaagaagct ttttctgcaa agatttcttt aatttacaat gggttatctt catttttgta
      661 gtata
//
LOCUS       DANJWC010000287          662 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.288, whole genome shotgun sequence.
ACCESSION   DANJWC010000287 DANJWC010000000
VERSION     DANJWC010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.288"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            30..>662
                     /locus_tag="PWX02_002436"
     CDS             30..>662
                     /locus_tag="PWX02_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730394.1"
                     /translation="MSIRWENIKPLQGSQNSAFEELVCQLARQEFKSQGKFTRISAPD
                     GGIEAMCELPDGSVCGWQAKYFLSSFSSSQWQQIEDSFKESLKNYPKLTKYYVCVATD
                     RANANVSGKKSFLTKWEEYTQRWKDFAQSQGREIEFEFWGSFELSDLLSKPENAGKKF
                     FWFNESELSDKWFEQYNQQAINNLGVRYTPEINVDLPIAMQLESLARTQIF"
BASE COUNT          237 a          104 c          121 g          200 t
ORIGIN      
        1 aattgaatat ttaattatgg taaaagaaaa tgtctattag atgggaaaac atcaagccct
       61 tacaaggttc tcaaaatagt gcttttgaag agctagtatg ccaattggca cgccaagaat
      121 ttaaatctca aggaaaattt actcgaattt ctgcacctga tggtgggatt gaagcaatgt
      181 gtgaattgcc agatggttcc gtatgtggtt ggcaagccaa atatttttta agctcatttt
      241 cttcatcaca atggcaacaa attgaagatt cctttaaaga atctttaaaa aactatccta
      301 aactaaccaa atactatgtt tgtgtggcaa ccgatagagc aaatgccaat gtatctggaa
      361 agaaatcatt tcttaccaaa tgggaggagt atacccaaag atggaaagac tttgctcaat
      421 cacaaggtcg tgaaatagaa tttgaatttt ggggaagttt tgaattatct gacttattat
      481 caaaacctga aaatgcaggc aaaaaattct tttggtttaa cgaaagcgaa ttatctgaca
      541 aatggtttga acaatacaat caacaagcca ttaataatct tggtgtgcgt tatacacctg
      601 aaattaatgt tgatttacct attgcaatgc aattagaaag cttggcaaga acccaaattt
      661 tc
//
LOCUS       DANJWC010000288          637 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.289, whole genome shotgun sequence.
ACCESSION   DANJWC010000288 DANJWC010000000
VERSION     DANJWC010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.289"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            95..>637
                     /locus_tag="PWX02_002437"
     CDS             95..>637
                     /locus_tag="PWX02_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDL1730395.1"
                     /translation="MLASILSGARGGWIAFPFCFLLILIYNLQHINKKLIIFISLLLS
                     VVLAIFIARPEFGIQQRYNEAKSDIIGYFEQGNKNSSLGARFDMWENTIAAITEKPIL
                     GHGGIGYDNFRERQMKANKMAKTTYFNSLHNQYLEASVKRGLIGFLALIAVLIIPFII
                     FTKRLKDNNIAIKCTAILGII"
BASE COUNT          226 a           98 c          102 g          211 t
ORIGIN      
        1 attaggatta ctcagtatat ccattgcatt ttattagttc gcagaaaaga aaaatagaat
       61 agcactctta tatattatct tcgctgcatt tggtttgtta gccagcattc taagcggtgc
      121 aaggggtggc tggattgcct ttcctttttg ttttctactc attctaatat acaatttaca
      181 acatataaat aaaaaactaa tcatattcat ctctttatta ttaagtgttg tattagctat
      241 atttatagct agaccagaat ttggtattca acaaagatat aatgaggcta aatcagatat
      301 aatcggctat tttgagcaag gaaataaaaa ttcctctctc ggtgcccgtt ttgatatgtg
      361 ggaaaacacc atagctgcaa ttacagaaaa accaatatta ggacatggtg gtatcggata
      421 tgataatttc agagaaagac aaatgaaagc aaataaaatg gcaaaaacaa cgtactttaa
      481 cagcttacat aatcaatatc tagaagcttc tgtaaagcgt ggccttattg gatttttagc
      541 attaatagca gttttaatca ttcctttcat catttttaca aaacgcttga aagataataa
      601 tatagcaata aaatgcactg ccattttagg gataata
//
LOCUS       DANJWC010000289          586 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.290, whole genome shotgun sequence.
ACCESSION   DANJWC010000289 DANJWC010000000
VERSION     DANJWC010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.290"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..>586
                     /locus_tag="PWX02_002438"
     CDS             <1..>586
                     /locus_tag="PWX02_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalt transporter"
                     /protein_id="HDL1730396.1"
                     /translation="LVAISLVTVIVVIFTLSRQYFNLALKWVERGSFGLMILFGIYWC
                     YQVYRTVNRARKPIIKSIRISQKKAGKLPLVTQKGHIHTENCSCRHKHLPTSNEMQQV
                     RDWKAQLMLVLSIGSRPCSGAILVLFLSYTLNLYAWGVISALAMAVGTGLTLSLFAYL
                     VIIARNKAVKVSQWYFSAQINRNIVWILKFTLGLA"
BASE COUNT          159 a          100 c          133 g          194 t
ORIGIN      
        1 gcttagtggc aatttcattg gtgacggtga ttgtggtgat ttttactctc tctcgccaat
       61 attttaattt agccctaaaa tgggttgagc gggggagctt tggtttgatg atcttgttcg
      121 gcatttactg gtgttaccag gtgtatcgaa cggtgaacag agcgcgaaag cctatcatta
      181 aatccattcg tatttcgcaa aaaaaagctg gaaaattacc gcttgtcacg cagaaagggc
      241 atattcatac cgaaaattgt agctgtaggc ataaacattt accgacttca aatgaaatgc
      301 aacaagtgag agattggaaa gcacagctga tgttggtatt aagtattggc tcaaggcctt
      361 gctctggcgc tattcttgtt ttatttcttt cttatacgct caatctctat gcttgggggg
      421 ttatttcagc attagctatg gcagtaggaa cagggctgac tttaagctta tttgcttatc
      481 ttgttatcat tgcgagaaat aaggcagtta aggttagcca atggtatttt tcagctcaaa
      541 ttaatcgaaa tatcgtttgg atattaaaat ttacacttgg actcgc
//
LOCUS       DANJWC010000290          576 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.291, whole genome shotgun sequence.
ACCESSION   DANJWC010000290 DANJWC010000000
VERSION     DANJWC010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.291"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..539
                     /locus_tag="PWX02_002439"
     CDS             <1..539
                     /locus_tag="PWX02_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDL1730397.1"
                     /translation="QDTFYVGNLKGVGRIYQQTFIDTYSKVAFAKLYTMKTAISAADM
                     LNDKVLPYFESQGLPMLRILTDRGSEYCGKVENHDYELYLAINDIEHSKTKVKHPQTN
                     GICERFHKTILQEFYQVAFRKKIYTDLTTLQADLDEWLMYYNHHRTHQGKMCCGRTPM
                     ATLLDGKRIWVEKNLSSN"
BASE COUNT          207 a           95 c          122 g          152 t
ORIGIN      
        1 cacaagatac tttctatgtg ggcaacctca aaggagtggg tcgtatttat caacaaacgt
       61 ttattgatac ttacagtaaa gtggcgtttg caaaacttta tacaatgaag accgctatca
      121 gtgctgcgga tatgctgaat gataaagtgt taccatactt tgaaagccaa ggtttaccga
      181 tgttacgcat attaactgac cgaggaagtg aatattgtgg caaggtagaa aatcacgatt
      241 atgagcttta tttagcaata aatgatattg aacacagtaa aaccaaggta aaacatccgc
      301 agactaacgg catctgcgaa cggttccata aaacaatctt acaagaattt taccaagtgg
      361 catttaggaa gaaaatttat acggatttaa cgacattaca agcggattta gatgagtggt
      421 taatgtatta taatcaccat cgaacacatc aaggaaaaat gtgctgtggc agaacaccga
      481 tggcaacctt acttgatgga aaacggattt gggtggaaaa gaatttaagc tcaaattaat
      541 ctgacagaca cggtaattta aaacggggga ctgtca
//
LOCUS       DANJWC010000291          550 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.292, whole genome shotgun sequence.
ACCESSION   DANJWC010000291 DANJWC010000000
VERSION     DANJWC010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.292"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..546
                     /locus_tag="PWX02_002440"
     CDS             <1..546
                     /locus_tag="PWX02_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730398.1"
                     /translation="NMPIIWKNFEENYLYNKKLDGLWSTNTNYIIDYKLLNIPEEQPN
                     VIIEFMVNKDDNSILGEMTSTALCELIPLTWSFRLESPKPTLLNLIFDRELKLSYLVS
                     GGYTELSTFKIKEINSNLGTLTLTTQEDKTGIFPPTIKLINFSSNIEDDIKEIEQSCL
                     NSRKQLWEKRRKMQEEQKIPN"
BASE COUNT          221 a           77 c           90 g          162 t
ORIGIN      
        1 aacatgccta ttatttggaa aaatttcgag gagaattacc tatataataa aaaactggat
       61 ggtttatggt ctacaaatac aaattacatt atcgactata aattattgaa catacctgaa
      121 gagcaaccaa atgttatcat tgaatttatg gttaataaag atgacaattc gattttaggg
      181 gaaatgactt caacagctct gtgtgagtta atacctctaa cttggagttt tagattggaa
      241 agccctaagc caacattatt aaatcttatt ttcgatagag agttgaaatt gagttatctt
      301 gttagtgggg gatataccga gctatcaaca tttaaaatta aagaaattaa cagtaatcta
      361 ggaacactaa cattaactac gcaagaagat aaaaccggta tatttcctcc gacaatcaaa
      421 ttaatcaatt tttcatccaa tatagaagat gatattaaag agattgagca aagctgttta
      481 aatagccgaa agcaattatg ggaaaaacga agaaaaatgc aagaggaaca aaaaattccc
      541 aattaatctt
//
LOCUS       DANJWC010000292          540 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.293, whole genome shotgun sequence.
ACCESSION   DANJWC010000292 DANJWC010000000
VERSION     DANJWC010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.293"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            82..>540
                     /locus_tag="PWX02_002441"
     CDS             82..>540
                     /locus_tag="PWX02_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011150324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1730399.1"
                     /translation="MFYSNNPLIKHKTGLLNLAEELGNISQACKVMGMSRDTFYRYQQ
                     AVEQGGVEALLNQNRRVPNLKNRVDEAIEQAVVKFALDNPAFGQVRVSNELRKQGIFV
                     SAGGVRSIWLRHHLANFKQRLIALEKLVAEQGIILSETQVQALERKKEDEI"
BASE COUNT          185 a           94 c          116 g          145 t
ORIGIN      
        1 tgacaattca ctctaaaaag tgagagtttc ccgtttagaa tatgagtgtc aaaaatcaat
       61 ctaaacaaaa aggaaactct catgttttat tctaacaacc ctctcattaa acacaagacc
      121 ggtttattaa atttagcaga agaactgggt aatatttctc aagcctgcaa agtaatggga
      181 atgagccgag atacattcta tcgttatcaa caagcggttg agcaaggtgg tgttgaagca
      241 ttgctgaatc aaaatagacg cgttcccaac ttaaaaaatc gtgttgatga ggcaatagag
      301 caagctgttg tgaagtttgc tcttgataac ccggcatttg gacaggtaag agtgagtaac
      361 gaactccgta aacaaggcat ctttgtttca gcaggaggtg tacgttcgat ttggttacgt
      421 catcatcttg ccaattttaa gcaaagatta atcgccctgg aaaaactggt tgcagaacaa
      481 ggtattatac tcagtgaaac acaggtacaa gccttagagc gtaagaaaga agatgagatt
//
LOCUS       DANJWC010000293          516 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.294, whole genome shotgun sequence.
ACCESSION   DANJWC010000293 DANJWC010000000
VERSION     DANJWC010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.294"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
BASE COUNT          170 a           67 c          102 g          177 t
ORIGIN      
        1 ctttagtggc attagctgtt gcttttttgg cttcagcaac ccaaactaaa cagcaagtaa
       61 gcatttgagg gagcagagcg aatagttgtt aatacctaca agttatagcg gtcagtatga
      121 ggataatatg gtttataaat tctaaaattt aaacgccctt taatgatgat ttaaagggcg
      181 taaattttat ttatttgttt cggtcatctt acttaaaatc aatgataagt tggtttgctt
      241 aactctaacc aattcagcta ggtttctgcg gcaaccattt gggcacccaa atattttgga
      301 gggtaatttg gaggtctaaa aaataggctg gtcaaaattt gagtttcgga aaaatatagg
      361 tggtttcgga aaaataaaat aaactgatta aataacacat tgattttatt ggaaaatttt
      421 aggtgctttt taattggttc acttagactt caaacgtgcg taatttttac gagtgaatta
      481 aagcgaagag gattaagttt ttaaatgaac ttaatc
//
LOCUS       DANJWC010000294          512 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.295, whole genome shotgun sequence.
ACCESSION   DANJWC010000294 DANJWC010000000
VERSION     DANJWC010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.295"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(<1..436)
                     /locus_tag="PWX02_002442"
     CDS             complement(<1..436)
                     /locus_tag="PWX02_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730400.1"
                     /translation="MSALINLFYLYDPWFFHFLRMSFVVGLVACGYLVFQIIKGKSNG
                     IIIPKDSLIAIIALIGFSIIPLLINSTKEIGVLAMYVKMLILFVFGIAIYNVFYHNQQ
                     GKTLFVRDLKIGIAIQWICGVTALLGLPFMVDFLLSSNAMMPR"
BASE COUNT          195 a           96 c           77 g          144 t
ORIGIN      
        1 aacgtggcat cattgcattg ctggataata aaaaatccac cataaacggc aaacctaata
       61 aggctgtcac accacaaatc cattgaatag caatgccgat ttttaaatca cgaacgaata
      121 aagttttacc ttgttggtta tgataaaaaa cattataaat cgcaatcccg aacacaaaca
      181 aaatcagcat tttgacatac attgccagca caccaatttc ttttgtactg ttaatcaata
      241 atggaataat gctaaagccg attaatgcaa taattgcaat caggctatct ttaggaataa
      301 taatgccatt cgatttgccc ttgattattt ggaaaaccaa atagccacaa gcaacaagcc
      361 cgactacaaa cgacattctt aaaaaatgga aaaaccacgg atcgtaaaga taaaataagt
      421 taatgagtgc tgacatttca gacctattat gaatttcgct gagtaaaatt acgtttaatt
      481 gccagtaatt ttttaagtgg atattttacg gc
//
LOCUS       DANJWC010000295          508 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.296, whole genome shotgun sequence.
ACCESSION   DANJWC010000295 DANJWC010000000
VERSION     DANJWC010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.296"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(71..355)
                     /locus_tag="PWX02_002443"
     CDS             complement(71..355)
                     /locus_tag="PWX02_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018653005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730401.1"
                     /translation="MANITLSHITPKHFFETVKLLKTNNHRIYLSVNTLEVRFQYDLS
                     ESREVSDKRFEQLEHFMYFLQELVNSDIPIELDEFIRTYRKSPKSRFFRR"
BASE COUNT          169 a           90 c           97 g          152 t
ORIGIN      
        1 taaaaaagtg ccaaagttgg caccaaagtt cgggtgttaa agttggcacc aaagttcggg
       61 tgttaccacc tcaacggcga aaaaatcgac ttttaggcga tttacgataa gtcctaataa
      121 actcatcaag ctcaatagga atatctgaat taaccaactc ttgcagaaaa tacataaaat
      181 gctccagttg ctcaaaacgt ttatcactca cttctctact ctcacttaaa tcatattgaa
      241 agcggacttc cagcgtatta actgataaat agatgcggtg gttatttgtt tttaagagct
      301 taacagtttc aaagaagtgt ttaggggtga tatgagaaag ggtaatattt gccataatga
      361 cctcgagagt cattatttcg cataacagca gattatgtgt aaattctcgt gcagtggggc
      421 aagcctatta tactgcactc gaattgtaac ataatctgta agtcattatg cgaaaattaa
      481 taatataagt ttttcatcaa tcggcggt
//
LOCUS       DANJWC010000296          503 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.297, whole genome shotgun sequence.
ACCESSION   DANJWC010000296 DANJWC010000000
VERSION     DANJWC010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.297"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..>503
                     /locus_tag="PWX02_002444"
     CDS             <1..>503
                     /locus_tag="PWX02_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDL1730402.1"
                     /translation="YQRNYAWGRGEIELLIQDIEEAQKKEEIQKESEKHRYYIGSLVV
                     SKRSDGYEVIDGQQRLTTLKLLLSYYNLKYSEQKHINLTFEHREASNYSLSNLTNSSK
                     ESDSIRLGYQIISNILEQKANLSGKENLDIKEFFTFLLNNVVILRTEVPEDTDLNHYF
                     EIMNNRG"
BASE COUNT          197 a           71 c           88 g          147 t
ORIGIN      
        1 tatcagcgta actatgcttg gggaagaggt gaaattgaat tactgattca agatattgaa
       61 gaagctcaga aaaaagaaga aattcagaaa gaatcagaaa agcaccgtta ctatattggt
      121 agtttagtag tatctaaacg atctgatggc tacgaagtta ttgatggaca acaacgatta
      181 acgactttaa aactattgtt aagttattac aatctaaaat attcagaaca aaagcatatt
      241 aatctgactt ttgaacatcg tgaagcatct aactacagct tatcaaacct cacaaatagt
      301 tcaaaagaga gtgatagcat caggttgggt tatcaaatta tctccaatat actggaacag
      361 aaagctaacc tatctggaaa agaaaattta gatattaaag agttcttcac attcttgcta
      421 aataatgttg ttattctcag aacagaagta ccagaagata cggatcttaa tcattatttt
      481 gaaattatga ataatcgtgg tga
//
LOCUS       DANJWC010000297          483 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.298, whole genome shotgun sequence.
ACCESSION   DANJWC010000297 DANJWC010000000
VERSION     DANJWC010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.298"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..>483
                     /locus_tag="PWX02_002445"
     CDS             <1..>483
                     /locus_tag="PWX02_002445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1730403.1"
                     /translation="SEVLERKSDEGNGENTNKDEDDKKKIISLLNRKYVENKNEDNKK
                     GYDEIKDGKFNSIIDFPNFLMIVLKIYKKGSDVKLNDKEMISEFRSLEKDEIIEFSLC
                     LLRCRLLFDRYIIKTKDDEDDWLLLGLKKYDDGNFKEISSFSRAFRKDDGSDNDEPAS
                     D"
BASE COUNT          199 a           32 c          100 g          152 t
ORIGIN      
        1 tcggaagttt tagagaggaa atctgatgaa ggtaatggag aaaatacgaa taaagatgaa
       61 gatgataaga aaaagattat ttcgttatta aatagaaagt atgttgaaaa taaaaatgaa
      121 gataataaaa aaggttatga tgaaattaaa gatggaaaat ttaactctat tattgacttc
      181 cctaattttc taatgattgt tttaaagatt tataaaaaag gaagtgatgt aaagcttaat
      241 gacaaagaga tgataagtga gtttagaagt ctagagaaag atgaaataat tgaattttct
      301 ttatgcttgc taagatgtcg tttgctcttt gatagatata tcatcaagac taaggatgat
      361 gaagatgatt ggctattatt aggtttaaaa aaatatgatg atgggaactt taaagaaata
      421 agttctttca gtagagcatt tagaaaagat gatggttcgg ataatgatga acctgcaagt
      481 gat
//
LOCUS       DANJWC010000298          469 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.299, whole genome shotgun sequence.
ACCESSION   DANJWC010000298 DANJWC010000000
VERSION     DANJWC010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.299"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(116..191)
                     /locus_tag="PWX02_002446"
     tRNA            complement(116..191)
                     /locus_tag="PWX02_002446"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(156..158),aa:Thr,seq:ggt)
     gene            complement(195..269)
                     /locus_tag="PWX02_002447"
     tRNA            complement(195..269)
                     /locus_tag="PWX02_002447"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(234..236),aa:Gly,seq:tcc)
     gene            complement(313..397)
                     /locus_tag="PWX02_002448"
     tRNA            complement(313..397)
                     /locus_tag="PWX02_002448"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(361..363),aa:Tyr,seq:gta)
BASE COUNT          146 a          107 c          107 g          109 t
ORIGIN      
        1 tttctaaaag cctcttaaac aaaaacggtt acaatggaac aaaagaaccc attaaccata
       61 atgtagaaat taataccaat aaaaatttgc gtgagaaaga tagaatttag agaagtggtg
      121 ctgataggcg gatttgaacc gccgacctca cccttaccaa gggtgcactc taccaactga
      181 gctatatcag cgcttggagc gggcagcggg aatcgaaccc gcatcattag cttggaaggc
      241 taaggtaata gccattatac gatgcccgct gttctaaatt cgtctgacct acgcgattca
      301 aaatatcaga gatggtggag ggagaaggat tcgaaccttc gaaggctgag ccaacagatt
      361 tacagtctgc cccctttggc cgctcgggaa cccctccacg ataattgaat acttatttac
      421 gcctgagtta atggtgccga ctaccggaat cgaactggtg acctactga
//
LOCUS       DANJWC010000299          425 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.300, whole genome shotgun sequence.
ACCESSION   DANJWC010000299 DANJWC010000000
VERSION     DANJWC010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.300"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            complement(153..>425)
                     /locus_tag="PWX02_002449"
     CDS             complement(153..>425)
                     /locus_tag="PWX02_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HDL1730404.1"
                     /translation="WQALRAKRFLAIKFKRQQVIGNYIVDFVCFSAKLVIELDGSQHL
                     DQQNYDNLRTHFLNGQGFRVLRFWNNQIHSDLEAVLDTIFYEITRG"
BASE COUNT          150 a           77 c           61 g          137 t
ORIGIN      
        1 tcaaaacaac taaaaaatta gcattctcct tttgtaggag aaaatgttgt tctttcaaaa
       61 ttctaaaata accgcaaaat taactctcgc cccttgtggg agagagacag ataaatcatt
      121 cgttcagaat gatttatcag agagaggggg aattaccctc tcgtaatttc ataaaaaatt
      181 gtatctaaaa cagcctctag atcgctatga atttgattat tccaaaatct taaaaccctg
      241 aacccctgac cgtttaaaaa atgagttcgt agattatcat agttttgttg atctaaatgt
      301 tgtgaaccat ctaattcaat aactaatttc gctgaaaagc aaacaaaatc gacaatatag
      361 ttacctataa cttgttgccg tttaaatttt atcgctaaaa atcgttttgc tcttaatgct
      421 tgcca
//
LOCUS       DANJWC010000300          399 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.301, whole genome shotgun sequence.
ACCESSION   DANJWC010000300 DANJWC010000000
VERSION     DANJWC010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.301"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            <1..>399
                     /locus_tag="PWX02_002450"
     CDS             <1..>399
                     /locus_tag="PWX02_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDL1730405.1"
                     /translation="KLDRLRVCFYAKAVEGEINNNWRSRDLDELVNQWSWSEKQIQLL
                     QNRQHWKIKQVMNHENMLIDHLLQSYRNLIHFARKFHINPSIMTNDTDILMRKLYSVF
                     EALPGKISLINHKISSNLAEKAITFIEAKV"
BASE COUNT          150 a           56 c           68 g          125 t
ORIGIN      
        1 tgaaattaga ccgcttgcga gtctgttttt atgcaaaagc ggttgaggga gaaataaata
       61 acaactggag aagtcgtgat ttagacgagc tggttaacca atggtcttgg tcagaaaaac
      121 aaattcaatt acttcaaaac cgtcaacatt ggaaaattaa gcaagtgatg aatcatgaga
      181 atatgctgat tgatcattta ctacaaagtt atcgaaattt aattcatttt gctcgaaaat
      241 ttcatattaa tccgagcatt atgacaaatg atactgatat tttaatgcga aaactttatt
      301 ctgtatttga agcacttcca ggaaaaatat ctttaattaa tcataaaata tcgtccaatt
      361 tagcagaaaa agcaattaca tttattgaag ctaaagttg
//
LOCUS       DANJWC010000301          384 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.denovo.302, whole genome shotgun sequence.
ACCESSION   DANJWC010000301 DANJWC010000000
VERSION     DANJWC010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.denovo.302"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            113..>384
                     /locus_tag="PWX02_002451"
     CDS             113..>384
                     /locus_tag="PWX02_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HDL1730406.1"
                     /translation="MELSLLNELELEELIVEANSNDNINFLNEVFLDKKVADIKKITD
                     FDKWNYLHRILCYFNPSVELVRFYISNGLDVNAQDKYGMTPLHYAM"
BASE COUNT          145 a           38 c           75 g          126 t
ORIGIN      
        1 agattggaaa atatgtctga aaatcgtagc cataagaatg agatgcctgg aaaaggtgat
       61 attcttgaga tcaggcaaga tatgcagaag tttatgaatg gagataaata gaatggaatt
      121 atcattacta aatgaattag aacttgaaga gcttattgta gaagctaact ctaatgacaa
      181 tattaatttt ttaaatgagg tattcttaga caaaaaagtt gctgacataa aaaaaataac
      241 tgattttgat aagtggaatt atttacatag gattttgtgt tattttaatc caagcgttga
      301 gcttgttagg ttctatatat ctaatggatt agatgttaat gctcaagata aatatggaat
      361 gaccccttta cattatgcaa tgag
//
LOCUS       DANJWC010000302         3311 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate 38523
            SAMN05326345-rid16800653.guided.194, whole genome shotgun sequence.
ACCESSION   DANJWC010000302 DANJWC010000000
VERSION     DANJWC010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN05326345
            Sequence Read Archive: SRR3822815
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001584995.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001584995.1
            Genome Coverage       :: 22x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/21/2023 03:23:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,454
            CDSs (total)                      :: 2,412
            Genes (coding)                    :: 2,344
            CDSs (with protein)               :: 2,344
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 35
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 38 of 68
            Pseudo Genes (incomplete)         :: 13 of 68
            Pseudo Genes (internal stop)      :: 21 of 68
            Pseudo Genes (multiple problems)  :: 4 of 68
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN05326345-rid16800653.guided.194"
                     /isolate="38523"
                     /isolation_source="nasopharynx"
                     /host="Bos taurus"
                     /db_xref="taxon:75985"
                     /country="USA: Kansas"
                     /collection_date="2013"
     gene            62..922
                     /locus_tag="PWX02_002452"
     CDS             62..922
                     /locus_tag="PWX02_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HDL1730407.1"
                     /translation="MSIKKVTLASLILLGLTACSSGGSGSSSNNVAKPNTKPQTTQQQ
                     TDKAAAEKAAKDKAAKEKAEAERLAAEKAAKEKAEAERLAAEKEVDPIAIIKAKGIDD
                     TNYGLKLGNSKETIEGKVTVRTQSYLYNQPYSVVTAQAVQRNGWENGNYIDKVDGTVT
                     VKGLKTEKLPTEGTATYSGKAFNHDGAKGFNGGDLTYNVNFSKRTGSGRVEGEFGNLI
                     VLEEGTINNTQISSTVHGNNPLNLSGKYTVEFFGKNAEEIAGEIQTKANLTGEVSNFG
                     LAGTRGDIQK"
     gene            1118..2527
                     /locus_tag="PWX02_002453"
     CDS             1118..2527
                     /locus_tag="PWX02_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL1730408.1"
                     /translation="MKKYPLLFSLFSPVVWAEKVIEHNTDPISFERQPQAVKFEQNVV
                     KNSQKPTAYQPAYSVNQNVEQQINQAILSQDWATLEPLLSRYPQTENFDPLLWHYAQA
                     ALAYAKKDHQTAVFHYRQLLAKPPSLVYPRFDLALILAEDQQFRAAEQEFKAIQAHLP
                     PELAKIARNTQVQIAENEAWQPDFYLQYTQTDNVNNASSSPIVNVNGRIFRKNADSLP
                     QSAKGVRYGLGLSKLHNVKGNHFVGLTLNYSGIYYWDNRDYREQSISVSPNYSYRTVA
                     YRLTFSPFIEQNWLGSSRYNVQFGATLNGLRYLNSQWLVSGSFTHLQKRYFEELTASR
                     YNSYQHQLSGGVQWQAVKNGRFFANLDGVREIAREKAQSSNKWLARVGAVFQGERWAT
                     QASIGFGKRYFADKHYLFGYKRLDTEYQANLAVWNRTWHWQGIVPKLNFRYQKIDSNI
                     ADFYPRQNKEVFFTLEKLF"
     gene            2672..3145
                     /gene="gpt"
                     /locus_tag="PWX02_002454"
     CDS             2672..3145
                     /gene="gpt"
                     /locus_tag="PWX02_002454"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL1730409.1"
                     /translation="MSEKYTNEKYVVTWDMFHMHARKLAENLLPATQWKGIIAVSRGG
                     LFPAAVLARELGIRHVETVCIASYDHDSQGELNVLHAADTDGEGFIVVDDLVDTGNTA
                     KEIRRMYPKARFVTVFAKPHGAPLVDDYVIDIPQETWIEQPWDLGITFVQPLARR"
BASE COUNT         1017 a          623 c          759 g          912 t
ORIGIN      
        1 gtacaatatg cgtgtgtttt atacacatct atttttgttt atcttacaaa aggaacattt
       61 tatgtctatc aaaaaagtaa ctttagccag tttgatttta ttgggtttaa cggcgtgtag
      121 tagtggtggt tcgggcagta gctcaaacaa tgtggcaaaa ccgaatacta aaccgcaaac
      181 tactcagcaa caaaccgata aagcggcagc agaaaaagca gcaaaagaca aggcagcgaa
      241 agagaaagca gaagcagaac gtttagcggc ggaaaaagcg gcgaaagaga aagcggaagc
      301 ggagcgttta gcagcagaaa aagaagtaga tcctatagct attattaaag caaaaggtat
      361 tgatgataca aattacggct taaaacttgg taatagtaaa gaaactattg agggtaaagt
      421 cacagtcaga acacagagtt atttatataa tcagccatat tctgtagtaa cagcacaagc
      481 agttcaaaga aatggctggg aaaacggtaa ttatattgat aaagttgatg gaacagttac
      541 agttaaagga ttgaaaacag agaaattacc aacagagggt actgcaacat attcaggaaa
      601 agcatttaac cacgatggag ctaaaggttt taatggtggt gatctaacct ataatgttaa
      661 tttctctaag cgtaccggtt caggtagagt tgagggagaa tttggaaact taatcgtatt
      721 agaagagggg acgattaata atactcagat ttcttcaaca gtacatggca ataatccttt
      781 aaacctttct ggaaaatata cagtcgaatt tttcggtaaa aatgcagagg aaattgccgg
      841 tgaaattcag acaaaagcaa atcttactgg ggaagtgagt aattttggac ttgccggcac
      901 tcgtggcgat atccaaaaat aaccttttct aacgttcaat gccgtttaac gaaaaattaa
      961 acggcatttg tcttgatatt gcttgtagat accataagac acaatagttt agatttcaat
     1021 ttacagcttc taataaccct ctcccacaag gggagagagt gataaatccc gtagaggcga
     1081 acatataggt ccgcccctac caattttctt tgtctttatg aaaaaatatc cccttttatt
     1141 ttcgctgttc agccccgttg tatgggcaga aaaggtgatt gaacacaaca ctgacccgat
     1201 ttcctttgag cgtcagccgc aagcggttaa atttgagcaa aatgttgtaa aaaattcaca
     1261 aaaaccgacc gcttatcagc ctgcgtattc agtcaatcag aatgttgaac agcaaattaa
     1321 ccaagcgatt ctcagccaag attgggcaac gcttgaaccg cttttaagcc gttatccgca
     1381 aactgaaaat tttgacccgt tgttgtggca ctatgcgcaa gccgcacttg cctatgcgaa
     1441 aaaagatcat caaaccgcag tttttcacta tcgccaactg ttagctaagc ccccgagttt
     1501 ggtttaccct cgttttgatt tggcgttgat tttggcagaa gatcagcaat ttcgtgcggc
     1561 agagcaggaa tttaaggcta ttcaagccca tttaccgccg gaacttgcta aaattgcccg
     1621 caatactcaa gtccaaattg ctgaaaatga ggcgtggcag cccgattttt atctgcaata
     1681 tacccaaaca gataacgtga ataatgcctc atcttcgccg attgtgaatg taaatgggcg
     1741 gattttccgc aaaaatgcag acagtttgcc acaatcagca aaaggcgtgc gttatggctt
     1801 agggttgagt aaattgcaca acgtgaaagg caatcatttt gtcggtttaa cgctcaatta
     1861 tagcgggatt tactattggg ataaccgaga ttaccgtgag caatccatat ccgtttcgcc
     1921 gaattatagc taccgcaccg tcgcttatcg gctgactttt tcgcctttta ttgagcaaaa
     1981 ttggctgggg agtagccgtt ataatgttca atttggggca acactcaacg gtttgcgtta
     2041 ccttaattcg caatggttgg tgtcggggag ttttacccat ttgcaaaaac ggtattttga
     2101 ggagctaacc gcaagccgtt ataacagcta tcagcaccaa ctcagtggcg gtgtgcagtg
     2161 gcaagcggtc aaaaatgggc ggttttttgc aaatttagac ggcgtgcgtg aaattgcacg
     2221 ggaaaaagca caaagctcga ataagtggct ggctcgagtg ggggcggttt ttcaaggcga
     2281 acgctgggca acgcaggcaa gtatcggctt tggcaagcga tattttgccg acaagcacta
     2341 tttgtttggc tataagcggt tagacacgga atatcaagcc aatttagcgg tttggaaccg
     2401 cacttggcat tggcagggca ttgtgccgaa gctgaatttc cgttatcaaa aaattgacag
     2461 caatattgct gatttttacc ctcgccaaaa caaggaagtc tttttcactt tagaaaagct
     2521 attttagtgt tggttttttg tgctaagcat acgattgcgt agctgccggc acaaaaaata
     2581 aatgaaatga tggcgaaaaa agcattttcg ggttatccta gtgcgatttt ttatatcggg
     2641 gtagccccca attttatatt aggttgtttc tatgagtgaa aaatacacaa atgaaaaata
     2701 tgtcgtgacg tgggatatgt tccatatgca cgctcgcaag ttggcagaaa atttattacc
     2761 tgccacacag tggaaaggga ttattgcggt gagccgtggc ggcttgtttc cggcagccgt
     2821 tttagcacgt gagttgggta ttcgccacgt agaaacagtg tgcattgcca gctacgatca
     2881 cgatagccaa ggtgagttaa atgtgttaca cgcagccgat accgatggcg aaggctttat
     2941 tgtggtagat gatttagtgg atactggcaa cacggcaaaa gaaattcgca gaatgtaccc
     3001 gaaagctcgt tttgtaaccg tgtttgcgaa accgcacggt gcaccgttgg ttgatgatta
     3061 tgtgattgat attccacaag aaacgtggat tgagcagcct tgggatttag gcattacctt
     3121 cgttcagcct ttggcacgta gataattttg tgtaatggac aatgaaagtt gtccattatt
     3181 ttttatttat tcctatgaaa tgtaggggcg aatcatcatt cgccctattc ccaaaatcaa
     3241 ataggtatat acaaataatg agtttaggca atctttatat tatttctgcc ccaagtggag
     3301 caggaaaatc t
//
